<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BMK4</accession>
    <name>A0A3B4BMK4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Geranylgeranyl pyrophosphate synthase</fullName>
            <ecNumber evidence="8">2.5.1.1</ecNumber>
            <ecNumber evidence="7">2.5.1.10</ecNumber>
            <ecNumber evidence="6">2.5.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Farnesyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Geranylgeranyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">GGPS1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>isopentenyl diphosphate + (2E)-geranyl diphosphate = (2E,6E)-farnesyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19361"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58057"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>isopentenyl diphosphate + dimethylallyl diphosphate = (2E)-geranyl diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22408"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:58057"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homohexamer; trimer of homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 22">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="8"/>
    <dbReference type="EC" id="2.5.1.10" evidence="7"/>
    <dbReference type="EC" id="2.5.1.29" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BMK4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000997"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012906.2">
        <property type="protein sequence ID" value="ENSPNAP00000000997.2"/>
        <property type="gene ID" value="ENSPNAG00000007868.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153498"/>
    <dbReference type="OMA" id="ANFAYFW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000009">
        <property type="entry name" value="Geranylgeranyl pyrophosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00062555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00072971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000997.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="303" mass="34676" checksum="E94755CFB318FF07" modified="2024-11-27" version="2">MLPSYFSATFPRARLKLKEDCSCRCKTTEQSTYSKRTVVIEVTEMLHNASLLIDDIEDSSKLRRGFPVAHSIYGVPSVINSANYVYFLGLEKVLMLDHPEAVHVFTRQLLELHRGQGMDIHWRDTYTCPSEAEYRAMVLQKTGGLFGLAVGLMQLFSNWKRDLKPLLDTLGLFFQIRDDYANLSSREYSANKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENVDIKRYCVDYLEKVGSFAYTRQTLRGLEAEAYRLIAELGGNPELEGLVEHLSRMYKEPEVAASTSSSNSRLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNM4</accession>
    <name>A0A3B4BNM4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RNF224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNM4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012294.2">
        <property type="protein sequence ID" value="ENSPNAP00000001357.1"/>
        <property type="gene ID" value="ENSPNAG00000008113.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411341"/>
    <dbReference type="CTD" id="643596"/>
    <dbReference type="GeneTree" id="ENSGT00510000050587"/>
    <dbReference type="OMA" id="WLCCWFC"/>
    <dbReference type="OrthoDB" id="252722at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16565">
        <property type="entry name" value="RING-HC_RNF224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053122">
        <property type="entry name" value="RING_finger_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47454">
        <property type="entry name" value="RING FINGER PROTEIN 224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47454:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="30"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="162" mass="18276" checksum="A3E1174C69E0B9D5" modified="2018-12-05" version="1">MMDDEVAVSEAPAEADEEALPSRDSRKLDCIICYSAYNLAERLPRKLYCGHTFCQACLRRLDTAINEQLWIPCPQCRQNTPLPRGGAIGLDLDLVAFLGVKAEAENQRSSPQKTQLETKSSFVKQPITDQPPSAWANVALAEPRFHRSPCCMRCVLCCWWWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BP48</accession>
    <name>A0A3B4BP48_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein phosphatase Slingshot homolog 1</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">SSH-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BP48"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011989.2">
        <property type="protein sequence ID" value="ENSPNAP00000001532.2"/>
        <property type="gene ID" value="ENSPNAG00000000809.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156133"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000004">
        <property type="entry name" value="Protein phosphatase Slingshot homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000423">
        <property type="entry name" value="Slingshot protein phosphatase 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043587">
        <property type="entry name" value="Phosphatase_SSH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043588">
        <property type="entry name" value="SSH-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864:SF7">
        <property type="entry name" value="PROTEIN-SERINE_THREONINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864">
        <property type="entry name" value="SLINGSHOT PROTEIN PHOSPHATASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23040">
        <property type="entry name" value="PH_SSH1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="20">
        <location>
            <begin position="197"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="256"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="318"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="551"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="557"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="573"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001532.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="606" mass="69320" checksum="5989CABBA3F9B14E" modified="2024-11-27" version="2">MYSIEYRWKRICKSSYSVFIYVLHNIPTSLELRLYIIINSLAVRLESNCLDYVRYMVVVYTNGHQNTEENILLGMDFTNNDSKMCSIGMVLPLWSDTKIHLDGDGGFSISTAERSHVFKPVSVQAMWSALQVLHKACEVSCRYNYIPGGMALTWMGFYESCNFSDQSCINEWNAMTNLETTRPDSPVLFSDQPTERERTECLIKTKLRSVMMIQDLENITSKEIRIELEQHMSLNLQEYKEFIDNEMLLILAQMDKATLVFDHLYLGSEWNASNLEELRECGIGYILNVTQEIDNFFLGAFCYYNVHVYDDEATDLLAHWNETYNFIVRAKKNNSKCLVHCKMGVSRSASTVIAYAMKEYGWSLEKAYHFVKQKRSIAQPNPTLMTQLAEYEGILDSNKLWQPGCDNNSLEDFQAAGACGSQMLPEQELCTWSHKDVSVYPCCGDEPTDHLNYNYYFRRLSDTALDSEPSTPVRGLPLLGMEHVFIEIEDVERDALLDDEAFPIAHLTLPNEGTAAQTCGRLEPLEDMRLRLEFSTVEEEDEEEAQKEEAEMVAMARGEETAPSGREEVSLANANRFNNENTNNSNRLAGKRSCPSGFDVSVKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BP92</accession>
    <name>A0A3B4BP92_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Interleukin-6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IL-6 superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548219.1">
        <property type="nucleotide sequence ID" value="XM_017692730.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BP92"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000296"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014096.2">
        <property type="protein sequence ID" value="ENSPNAP00000000296.1"/>
        <property type="gene ID" value="ENSPNAG00000007421.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424599"/>
    <dbReference type="CTD" id="100270759"/>
    <dbReference type="GeneTree" id="ENSGT00390000017328"/>
    <dbReference type="OMA" id="MGLCYCW"/>
    <dbReference type="OrthoDB" id="8841348at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005138">
        <property type="term" value="F:interleukin-6 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045639">
        <property type="term" value="P:positive regulation of myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040117">
        <property type="entry name" value="GCSF/MGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003574">
        <property type="entry name" value="IL-6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030474">
        <property type="entry name" value="IL-6/GCSF/MGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10511">
        <property type="entry name" value="GRANULOCYTE COLONY-STIMULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10511:SF2">
        <property type="entry name" value="GRANULOCYTE COLONY-STIMULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00489">
        <property type="entry name" value="IL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00433">
        <property type="entry name" value="IL6GCSFMGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00126">
        <property type="entry name" value="IL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0011">Acute phase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017255535" description="Interleukin-6" evidence="5">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="214" mass="23960" checksum="2969FB8B5E73690E" modified="2018-12-05" version="1">MHLFLELHLALCVLSLVSPAPIQEPWKDMQDPEFQEAVESGHSLIRKILDDITELHNLWIPKGLSLDKKDSSKLKFLKTHLNLPEAPAIKPLSDSFTLGACLSQIATGLKLHQTLLTVVSKLSPAKSEMVDGLLCDIRDLLLQVRKMQDLKNLLSKPEPQPVSESQLEETLAPKLSDEYKKHVAAHLTLLQLRDFSQEVSRTFRSMTVPSNSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BR86</accession>
    <name>A0A3B4BR86_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase I subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">POLR1E</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564836.1">
        <property type="nucleotide sequence ID" value="XM_017709347.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BR86"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002148"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010938.2">
        <property type="protein sequence ID" value="ENSPNAP00000002148.1"/>
        <property type="gene ID" value="ENSPNAG00000006987.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434317"/>
    <dbReference type="CTD" id="64425"/>
    <dbReference type="GeneTree" id="ENSGT00390000018004"/>
    <dbReference type="OMA" id="DVYPFDE"/>
    <dbReference type="OrthoDB" id="532500at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="46103" checksum="5935E13388C1CA9B" modified="2018-12-05" version="1">MATCVWTPCEGRAETEEDGAVIVKFSNGHVKNTDQLDFRLFKHIDGVNPRKKSRRIVAAESDRLAYVGSNFGSGSLQCNNLCRYFVGVLDKQTMEMKVQRAQIFNLQPSIPGETVVNDDKNETKSYRQKVDDLIEAFGTTRQKRALSSRRLNEVGNETLQKAVDRAAGNVIDQKGLEVLQQEVADSEAQTEVALFLPPFNKNADKREDVYPFSGLLSPHELDALKQAGEKMAGLTSEDLLKMRKDSSPETVLRLLECLPKDPEARNRQACCAWYLLFLIRLAQQKKLYYKLDEDDCPRLIYNKVRKIFTVDTFAKGRVKSTVSASMQVKLASHCLALLLHMGDQTANLTLLHRDLNISERKMLEVAKAMGLTLSRPQEEHRMAFLELPLVRYDRHPEERKRKKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSK0</accession>
    <name>A0A3B4BSK0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-serine deaminase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-threonine dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SDSL</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_017541127.1">
        <property type="nucleotide sequence ID" value="XM_017685638.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BSK0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001449"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012132.2">
        <property type="protein sequence ID" value="ENSPNAP00000001449.2"/>
        <property type="gene ID" value="ENSPNAG00000007158.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413229"/>
    <dbReference type="CTD" id="113675"/>
    <dbReference type="GeneTree" id="ENSGT00940000160713"/>
    <dbReference type="OMA" id="DGWVNIH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF16">
        <property type="entry name" value="SERINE DEHYDRATASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="6">
        <location>
            <begin position="6"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001449.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="332" mass="34874" checksum="F81793A3E5AC2AB3" modified="2024-11-27" version="2">MPGIQGFHINSPLVESTAMAKRLGTTVYLKMESSQPAGSFKIRGIGHLVCKLAGSNSHGVVCASGGNAGLAAAYAARKLNIPATIILPSPSLQLVTEKLQDQGATVKCVGKVWDDANAEALRLAESENLVYIPPFDHPLLWEGHSSVVHELKASLPCRPGAVVLSVGGGGLFCGVMHGLDEVGWGSVPVLCMETFGADCLNAAIKAGHLVTLPDITSEAKCLGAKTACSAAFEYSKRSNVISELVTDRQALEAVELFLDEERVLVELACGASLAAVYSGVIQRLQDEGRLPKPLGLLVVIVCGGSGVSLAQLQHLRTVCKSENYNSHDHVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVW6</accession>
    <name>A0A3B4BVW6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Adrenomedullin 2b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenomedullin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554454.1">
        <property type="nucleotide sequence ID" value="XM_017698965.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVW6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002645"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010093.2">
        <property type="protein sequence ID" value="ENSPNAP00000002645.1"/>
        <property type="gene ID" value="ENSPNAG00000008980.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428172"/>
    <dbReference type="CTD" id="403114"/>
    <dbReference type="GeneTree" id="ENSGT00940000154380"/>
    <dbReference type="OMA" id="RGGHHYP"/>
    <dbReference type="OrthoDB" id="9907777at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010460">
        <property type="term" value="P:positive regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003073">
        <property type="term" value="P:regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051665">
        <property type="entry name" value="Adrenomedullin-reg_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414">
        <property type="entry name" value="ADRENOMEDULLIN, ADM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414:SF2">
        <property type="entry name" value="PROTEIN ADM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017267792" description="Adrenomedullin 2b" evidence="6">
        <location>
            <begin position="23"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18981" checksum="71912272D11F0C00" modified="2018-12-05" version="1">MKALLLPLTLYCISLFSLQLQALPVGDRLDLMTRLSQLREGSPESDPGSVDATANPVVPLDRTALWRALLFRKPPASFEDPMSIFRVPHTSRPVAGASTEKGEGPNQTGLRSRRHAFIRGHHPQRHGQLMRVGCVLGTCQVQNLSHRLYQLVGQTGREDSSPVNPRSPHSYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXN6</accession>
    <name>A0A3B4BXN6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564734.1">
        <property type="nucleotide sequence ID" value="XM_017709245.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BXN6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009062.2">
        <property type="protein sequence ID" value="ENSPNAP00000003244.2"/>
        <property type="gene ID" value="ENSPNAG00000009660.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434244"/>
    <dbReference type="GeneTree" id="ENSGT00950000183019"/>
    <dbReference type="OMA" id="NQHDQTA"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000003">
        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000004">
        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF122">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="14">
        <location>
            <begin position="262"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="14">
        <location>
            <begin position="344"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="14">
        <location>
            <begin position="417"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="14">
        <location>
            <begin position="486"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003244.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="558" mass="62215" checksum="15FD28518F389280" modified="2024-11-27" version="2">MGSTAMDSKAKDSGKKSTRKRRTLRLNMPDLSTFAMPFLEGDSDHAEKDSVRKGEISCHSASPTKGFFSRGPFSRPSSPMSAPARSRNSPGSPKTIFPYPPQQESSPKSPRRLSFSGIFRSSSKDSSSTSVSTSTSTSPVTIKLFSRARKASSLSSPPTTPTQVTKQPPPFPLESNRSDPEQPEERLRSLSSPLDTGQRFSLPSAKPPMTPTSAPISTSRTVHGLPEGMLEKLDLEDEAVEESESDIYMRFMKSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWETKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASIFDAVYSLIKNKIHRLPVIDPVSGNALYILTHKRILKFLQLFVCEMPKPAFMKQTLEELGIGTYSNIAFIHPDTPIIKALSIFVERRVSALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDISVTQALMHRSQYFEGVMKCNRLETLETIVDRIVKAEVHRLVVVDENACIVGIVSLSDILQALVLTPAGLNRKENEVEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0W2</accession>
    <name>A0A3B4C0W2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0W2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006820.2">
        <property type="protein sequence ID" value="ENSPNAP00000004550.2"/>
        <property type="gene ID" value="ENSPNAG00000010995.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004550.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="118" mass="9565" checksum="4F84D755B830BEC1" modified="2024-11-27" version="2">MAKGSGLGSAAKGSGLGSAAKGSGLGSAAKGSGLGSAAKGSGLGSAAKGSGLGSAAKGSGLGSAAKGSGLGAAAKGSGLGAAAKGSGLGAAAKGSGLGGSGLGAAAKGSGLGAAATCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C153</accession>
    <name>A0A3B4C153_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome activator complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Proteasome activator 28 subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PSME2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of PSME1 and PSME2, which forms a hexameric ring.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560022.1">
        <property type="nucleotide sequence ID" value="XM_017704533.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C153"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005493"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005205.2">
        <property type="protein sequence ID" value="ENSPNAP00000005493.1"/>
        <property type="gene ID" value="ENSPNAG00000006588.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431412"/>
    <dbReference type="CTD" id="5721"/>
    <dbReference type="GeneTree" id="ENSGT00950000183098"/>
    <dbReference type="OMA" id="KKPPKCG"/>
    <dbReference type="OrthoDB" id="6591885at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008537">
        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.180:FF:000002">
        <property type="entry name" value="Proteasome activator complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.120">
        <property type="entry name" value="Proteasome activator pa28, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036996">
        <property type="entry name" value="PA28_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009077">
        <property type="entry name" value="Proteasome_activ_PA28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003185">
        <property type="entry name" value="Proteasome_activ_PA28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660:SF6">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660">
        <property type="entry name" value="PROTEASOME REGULATOR PA28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02251">
        <property type="entry name" value="PA28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="11">
        <location>
            <begin position="94"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="60"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="239" mass="27351" checksum="6AB1E3CDEA08725D" modified="2018-12-05" version="1">MSKFKINRENAVKVDNFRKTLYQQAEDLFSNHIPLKISRLDNLLQSDEFCVSDLTTLHAPLDIPIPDPPPPEDEEMETDKNEEEKKKPPKCGFIKGNEKIVKLLDIVKPEITSLKETIITVACWISHLIPKIEDGNDFGVAVQEKILERIAAVKTKVEGFQTNINKYFSERGDAVAKASKETHVMDYRALVHEKDEATFSEIRVIMLDIRGFYAELYDIISKNLEKVTNPKGEEKPSMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C285</accession>
    <name>A0A3B4C285_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 2 subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551158.1">
        <property type="nucleotide sequence ID" value="XM_017695669.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C285"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005893"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004507.2">
        <property type="protein sequence ID" value="ENSPNAP00000005893.1"/>
        <property type="gene ID" value="ENSPNAG00000012003.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426300"/>
    <dbReference type="CTD" id="321564"/>
    <dbReference type="GeneTree" id="ENSGT00390000007015"/>
    <dbReference type="OMA" id="WDWLHEL"/>
    <dbReference type="OrthoDB" id="1685042at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000795">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="12">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="315" mass="36156" checksum="67ECCA1713830CF4" modified="2018-12-05" version="1">MPGLSCRFYQHRFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLYQRTAWVFDEKFKRPGYGAYDVFKQAVSDPSILDGLDLTEEERAVLIDNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLKCSTDAMPIKINLIAPPRYVMTTTTLERTEGLSMLNQAMAVIKEKIEEKRGVFNIQMEPKVVTDTDETELQRQLERLERENAEVDGDDDAEEMEAKAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3J7</accession>
    <name>A0A3B4C3J7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">MARCKS-like 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MARCKS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576471.1">
        <property type="nucleotide sequence ID" value="XM_017720982.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3J7"/>
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    <dbReference type="Ensembl" id="ENSPNAT00000005762.2">
        <property type="protein sequence ID" value="ENSPNAP00000005169.1"/>
        <property type="gene ID" value="ENSPNAG00000001740.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000165880"/>
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    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:cytoskeleton"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072554">
        <property type="term" value="P:blood vessel lumenization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:central nervous system development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003407">
        <property type="term" value="P:neural retina development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002101">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353:SF8">
        <property type="entry name" value="MARCKS-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353">
        <property type="entry name" value="MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE MARCKS"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MARCKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="102"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="20492" checksum="930D491A63F45AAF" modified="2018-12-05" version="1">MGAQLTKGEAETAAAKANGQENGHVKTNGDVSAKPEGEAVAADGNGTAEVAKETTEAGDAIEAAPAPATEAEAPKADGEASKEATKKKKKFSLKNSFKFKGISLKKSKKGSEDAAEGAASPSVEENGHAAKEAKEEEPAAEPAAETTEAEAEAAVAVEAAPEAEAEAKAEEPAPAAEAAPTEESAKSEETPAEPAPATTEEPAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C519</accession>
    <name>A0A3B4C519_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TMA16</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the biogenesis of the 60S ribosomal subunit in the nucleus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with pre-60S ribosomal particles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMA16 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017717458.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000005674"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004897.2">
        <property type="protein sequence ID" value="ENSPNAP00000005674.2"/>
        <property type="gene ID" value="ENSPNAG00000001948.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000068014.1">
        <property type="protein sequence ID" value="ENSPNAP00000049322.1"/>
        <property type="gene ID" value="ENSPNAG00000001948.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000079004.1">
        <property type="protein sequence ID" value="ENSPNAP00000045929.1"/>
        <property type="gene ID" value="ENSPNAG00000001948.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439196"/>
    <dbReference type="CTD" id="55319"/>
    <dbReference type="GeneTree" id="ENSGT00390000004179"/>
    <dbReference type="OMA" id="SWFLGQI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.170:FF:000001">
        <property type="entry name" value="Translation machinery-associated 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005674.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="207" mass="23996" checksum="C2B2ACBD12C8B2C1" modified="2024-11-27" version="2">MPKAVKAKAKAKAKVENKVIHPYSRKAAYLARDAAKHSRKEKSKSEKAQRLNLIREKLLWFQSQLDAEKLEYTKKDACEIIERYLHRFDDELEQIELANSIKGRQGRLHGSREAAIKQTIERERIQFEGCGFEIPDIINSKHLRTFREWDGDMRKLPNIKMRKVSAKGSESKHNAVQEVHIEEEQLLDSEENEVSQDSDGDELLGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8N0</accession>
    <name>A0A3B4C8N0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017572048.1">
        <property type="nucleotide sequence ID" value="XM_017716559.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017572051.1">
        <property type="nucleotide sequence ID" value="XM_017716562.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8N0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027788"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001665.2">
        <property type="protein sequence ID" value="ENSPNAP00000007545.1"/>
        <property type="gene ID" value="ENSPNAG00000002706.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438627"/>
    <dbReference type="GeneTree" id="ENSGT00390000017874"/>
    <dbReference type="OMA" id="NGMESAG"/>
    <dbReference type="OrthoDB" id="9898538at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026152">
        <property type="entry name" value="SARG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21555">
        <property type="entry name" value="SPECIFICALLY ANDROGEN-REGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21555:SF1">
        <property type="entry name" value="SPECIFICALLY ANDROGEN-REGULATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15385">
        <property type="entry name" value="SARG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="499" mass="53782" checksum="A2CDEF7E230A3A34" modified="2018-12-05" version="1">MPKSDTWPGGIATETVGEMDSAESCDSVVSINSCFSDDSLQYLSAEEKACLMFLEETIESLDTEDDSGLSNDEAESLPVPGNVAIKTAHLSTSMNQNKPKDVPKHYIDDPRMFGSSEHNQILNYLVPTPFVLANNNAHIPPKSADSKEKLPSKEVISHKSDRDGPRVPSEVNVIVIPPPVMVKSSNERDRGLQNDQSPRGPLTYEGLVQLRKKVALRRNLEDEKRPSTHSNNGTKVFSQNIQPRENQGSKNNPPAVAPKPKKIPSHIAVLNHKGTTASTADSTMHCLSTSPGSRGLMKPEKVRLDALSKLGLLKESDSKPSTIGHHSEGLQPSSGSVPGLSQTRPLPPCEAVRNQESRNQPSQARSVLHHRSMTDLATVPPQLHHVKLAGGKAATLERSGMGWGSSMSSYEISECQSSAASQNHLRCSSTVVSTSNLGNIQTGRTTVSTDKAQAASGQSSTVPPVPHKPLHALGLNVLVVPNMGEDRREALRKLGLLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CBG2</accession>
    <name>A0A3B4CBG2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcyclin-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CACYBP</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CBG2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008615"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034979.2">
        <property type="protein sequence ID" value="ENSPNAP00000008615.2"/>
        <property type="gene ID" value="ENSPNAG00000014239.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435255"/>
    <dbReference type="CTD" id="27101"/>
    <dbReference type="GeneTree" id="ENSGT00390000016470"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044548">
        <property type="term" value="F:S100 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06468">
        <property type="entry name" value="p23_CacyBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000006">
        <property type="entry name" value="calcyclin-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037201">
        <property type="entry name" value="CacyBP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052289">
        <property type="entry name" value="Calcyclin-binding_UBL-bridge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037893">
        <property type="entry name" value="CS_CacyBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015120">
        <property type="entry name" value="Siah-Interact_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13164:SF3">
        <property type="entry name" value="CALCYCLIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13164">
        <property type="entry name" value="CALICYLIN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05002">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09032">
        <property type="entry name" value="Siah-Interact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140106">
        <property type="entry name" value="Calcyclin-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CS" evidence="12">
        <location>
            <begin position="72"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="SGS" evidence="11">
        <location>
            <begin position="150"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008615.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="226" mass="26084" checksum="2C567DD74C65B0A8" modified="2024-11-27" version="2">MDLHEQISGLENDLKEVTRLLGLCESKRVQDVLSQEQKKIEKELSQKRLQTEQQAKRDSGDKGDTTVKGYTVKINNYGWDQSDKFVKIYITLKGVHKITAENIEAIFTERSVNVLVKNLDGKNHQMMINNLLCPIVVEESSRKVKTDMVLVMCKKNMAKKWECLTQVDKQTKEKDKPSYDENADPGEGLMSMLKKIYSEGDDEMKRTINKAWAESQEKKARDDFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CKK8</accession>
    <name>A0A3B4CKK8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Selenocysteine-specific elongation factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Elongation factor sec</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Eukaryotic elongation factor, selenocysteine-tRNA-specific</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">EEFSEC</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons. Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017574753.1">
        <property type="nucleotide sequence ID" value="XM_017719264.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CKK8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011064"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017916.2">
        <property type="protein sequence ID" value="ENSPNAP00000011064.1"/>
        <property type="gene ID" value="ENSPNAG00000016724.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440407"/>
    <dbReference type="CTD" id="60678"/>
    <dbReference type="GeneTree" id="ENSGT00940000158170"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04094">
        <property type="entry name" value="eSelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01889">
        <property type="entry name" value="SelB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03696">
        <property type="entry name" value="SelB_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000900">
        <property type="entry name" value="Eukaryotic elongation factor, selenocysteine-tRNA-specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000052">
        <property type="entry name" value="Selenocysteine-specific elongation factor EF-Sec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049394">
        <property type="entry name" value="eEFSec_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049393">
        <property type="entry name" value="eEFSec_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21131">
        <property type="entry name" value="eEFSec_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21208">
        <property type="entry name" value="euk_SelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="19">
        <location>
            <begin position="15"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="531"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="584" mass="63789" checksum="8E24E741A879D472" modified="2018-12-05" version="1">MADPSANVGESLPRILNFNVGVLGHVDSGKTSLARALSSTASTAAFDKNPQSKERGITLDLGFSAFTVPLPEHLREACGERQYDSLQFTLVDCPGHASLIRTIIGGAQIIDLMLLVVDVVKGMQTQTAECLLIGQLTCSRMIVILNKTDLLPSDKKQNAIDKMTKRMHKTLENTRFKGCPVIAVAAKPGGPDAPGTEEAQGVTELIELLKSQAFLPHRDPSGSLLMAVDHCFSIRGQGTVITGTILQGALRVNDSLEIPALKVTRKVRSIQMFRKPVPSVMQGDRVGVCVTQFDPKLLERGVVCTPGSLRTLHAAVISVQKIEYYRGALSSRSKIHITVGHETVMARVTFFSRASHSGQSGASDGSPSEQSLDTFSFDWEFQHQDEFLSGQAEVEGQAPQQWALLEFERPVTCPPLCLVIGSRLDSDIHANTCRLAFHGKLLEGFEDKNYTETTLPRLKISKDKHKEGAVERVTDDYTVIGKNLFKKETNIQLFVGLKVTLSSGETGVIEGGFGQSGKIKIRVPEGLKGETKQLLSSSSKKKGKGVAKNEQTKDEEGKTDSQPIIIHLNFKRYVYDPHKKMVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CKN0</accession>
    <name>A0A3B4CKN0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger protein 276</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017572696.1">
        <property type="nucleotide sequence ID" value="XM_017717207.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CKN0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011913"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019034.2">
        <property type="protein sequence ID" value="ENSPNAP00000011913.1"/>
        <property type="gene ID" value="ENSPNAG00000017603.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439037"/>
    <dbReference type="CTD" id="92822"/>
    <dbReference type="GeneTree" id="ENSGT00940000158841"/>
    <dbReference type="OrthoDB" id="8823111at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000219">
        <property type="entry name" value="Zinc finger protein 276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000400">
        <property type="entry name" value="Zinc finger protein 276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000503">
        <property type="entry name" value="Zinc finger protein 276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF53">
        <property type="entry name" value="CHORION TRANSCRIPTION FACTOR CF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07776">
        <property type="entry name" value="zf-AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="20">
        <location>
            <begin position="52"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="289"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="127"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="444"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="414"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="596"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="638" mass="70114" checksum="54D09DB9AD01F128" modified="2018-12-05" version="1">MQRGQGQAVRRRRAEHSDTLKPPKPKQVRPASGEHRPAGPVKTVKVDKGATGQCRLCHGHFSTRRLRRVFGKPPDSEQFCRDFQRLLGVPLLRDPSLSQFICRTCYGQFYKCLAVLTNFRARVNLTPNSTITDSRPTKWPSSKDDKNRARVQIQGRIPSPVQSDDSDLSDSSLSSDEDDDDDDDDDDDDDEDKRKSSSSDDSSDPYAENRVSSSKKISKETIKKPVKLKILQPKERKKPGPKPGWKKKAGLEGEAVVYTCHHQGCSAVYKTRDGLKKHIREHHEDVRERPCPHPGCNKVFMIDRYLQRHMKLIHTEVRNYVCEQCGQTFKQRKHLQVHQMRHSGAKPLQCEVCGFQCRQRASLKYHMVKHQGEEDLEFACALCRKRFEKAHNLNVHMSMVHPLLQGGGIMLPDDPEHPMHPDPADLDVKEGGIVMPADPADLAAEGVTLPSGPDNHDPGAERGGVTLPSDPDNHEPSEVAAEGGEVTLPSDPDNVATEGGEVTLPSDHDNHDPGDVTVEEGEVTLPSDPDSGDLAAEGSEVTLPSDPDNHDPGDVAVEGGEVTLPSDPDSGDLATEGGEVTLPSDPDPDPSTQEDEIARPSDPDGHDPGELTAQTNADGAEMNCELPAIHSDLIQPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNP4</accession>
    <name>A0A3B4CNP4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNP4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021318.2">
        <property type="protein sequence ID" value="ENSPNAP00000013662.1"/>
        <property type="gene ID" value="ENSPNAG00000019621.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161337"/>
    <dbReference type="OMA" id="INVALMQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF998">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="53"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="229" mass="26442" checksum="FC194244D17B9BAE" modified="2018-12-05" version="1">MNLTQSNITQTKGKDSYMVAVTKNVIVVALYFSINYINGTLLTIAVLVNLISYILYTINVSRYIAICNPLRHMQICTVCRTYVLIGLIWVLDAVTILPDLFILLATEPLSFFHSTIYCSRDTVFQHPYLLEKRKVSYLVYLSFVWFTLFYTYFRIMFTAKATGADAQKAHNTILLHGLQLLLTFISLFPMILNDIRFTSYIFVHILPRFISPIHLLCNKLKEKNKTQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRG1</accession>
    <name>A0A3B4CRG1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Klotho</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KL</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572595.1">
        <property type="nucleotide sequence ID" value="XM_017717106.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CRG1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014682"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022647.2">
        <property type="protein sequence ID" value="ENSPNAP00000014682.1"/>
        <property type="gene ID" value="ENSPNAG00000020632.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438962"/>
    <dbReference type="CTD" id="9365"/>
    <dbReference type="GeneTree" id="ENSGT00940000157614"/>
    <dbReference type="OMA" id="RKPHCVD"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000042">
        <property type="entry name" value="Klotho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000062">
        <property type="entry name" value="Klotho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF10">
        <property type="entry name" value="KLOTHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017370024" description="Klotho" evidence="11">
        <location>
            <begin position="46"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="982"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1009" mass="115039" checksum="6EB25863FC022335" modified="2018-12-05" version="1">MIDRAEEKLRVSCSNGRSDRDQSTTRASMLLLLFALCCALPRAWAEPGAGLRTWTRFARLPYPADKAFLHDTFPSGFAWAAGTAAYQVEGAWQKDGKGKSVWDTFTRGGARVLTGDVGSDSYHNVRADVRALSQLGVTHYRFSLSWPRIFPDGDAEGARNARGVQYYRELLRELRDIGVQPVVTLYHWDLPDSLQRRYGGWNSSELVGLFARYADFCFSTFGDDVKLWITMDNPFVVAWHGYGTGVVAPGVKGDPDMPFRVGHHLLKAHAAAWHLYDEHYRAKQGGKVTIALASHWIKPSSTRLESHQACQCSLDFVLGWFARPLFVDGDYPPCMKRNLTHRLPSFTKEERAYVNGTADFFALSHGPALSFQLINDSLRFGQTEDLDLRMLLYWVHAEYNNPPIFVVESGWFVSGNTKTKDAKHMYYLKRFIMETLKAIRFDRVNVVGYTAWSLLDGFEWYREYGIRRGLFYVDFNSEHLTREPKTSALFYSKLIEKNGFPELPENRPAVGVFPCDFAWGVASNSIQVETTPTQFVDPGVYIWNISDNGKLKRLESVQIPVHRMRHCADYAAIRQQVSEIQAMHVSHFQFSLNWSSVVPTGQISEANNTLLSYYHCFVSELQRANITPVVTLWHHTGKLSSLPAPLEANGGWQSDETVQAFVDYARLCFRRLGAHVKLWITLNEPNEEGLCYAVGHQLLRAHALAWRVYDNEFRKSQGGKASLVLHMDWVEPAFSFSREDVAPANRVLDLRVGWFAEPIFGAGDYPPVMRRWLQQRNTIDLFNYHLPTFSEEDRQLVKGTYDFFAISHFTTSMVYDGVEDKYLYKDSLEVQMISDVTWIMSPRRNSPVVPWGLRKALNWVNSHYKGVPIYVMANGVQEDTARFRDSLRVYYLYNYINEALKAYMLDGVNLKGYFAYAFNDQRDPGFGMYGNVQEEVIVKDSLNHYRNIIDHNGFPAPNSPAQQCLHTPDHCPVCQVLAKQPVVGFISLVGSSVLITLGLVIYYGAKRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSX9</accession>
    <name>A0A3B4CSX9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_017574318.1">
        <property type="nucleotide sequence ID" value="XM_017718829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CSX9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013639"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021295.2">
        <property type="protein sequence ID" value="ENSPNAP00000013639.1"/>
        <property type="gene ID" value="ENSPNAG00000019508.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440103"/>
    <dbReference type="GeneTree" id="ENSGT00390000001775"/>
    <dbReference type="OMA" id="QDIRYWG"/>
    <dbReference type="OrthoDB" id="445007at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883:SF52">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT HYPOPHOSPHITE DIOXYGENASE-LIKE GENE A2 [PROVISIONAL]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="31690" checksum="CC1FABC09A209461" modified="2018-12-05" version="1">MSGKMTDVQAQYEKQGYLTSIPILSAEELQQARDAFALLEKEFGKDYTSYSLHNVHMEYDWVMALTKHPRVLEVITTVLGPDVILLDSRFICKYPVSKRSPAKAGDSNDVTAYNQESEAEKSMSDQNSDTGLPYVAWHQDMKYWGLDGGPVVSVWLALDDSLEDNGALQVIPGTHCSGLLPHRPAKRAGNMLSVNQEIPEELVQKENSLLCPLLAGQMSIHDGLLVHASDPNTSERRRCGFVIRYVPAAAYPIQDPERPRSFPATVVVSGQDKYNHFSKQRPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTG9</accession>
    <name>A0A3B4CTG9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">E3 ubiquitin-protein ligase TRIM39-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTG9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014690"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022761.2">
        <property type="protein sequence ID" value="ENSPNAP00000014690.2"/>
        <property type="gene ID" value="ENSPNAG00000003655.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165127"/>
    <dbReference type="OMA" id="RAELMIR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="67"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="223"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="438"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014690.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="72943" checksum="613B5B72CBF3F00D" modified="2024-11-27" version="2">MRTDNRKGEQRRREQTTGEESREQERKEERTGEENRVELERRGDGRRQYKRVMPFLGSFLSEEQFQCSICLDIFDNPVSTPCGHSFCMTCIGYYWQGVKMCQCPLCKENFKKKPELHINRTLREITEQFKQMKGDAGACGGLEWKGVEGKRIEERPLKQRQLSGDLFLVMKRKLLKPFPSKVSQNDSHTMVIQGTDSDIIQPQELTGGVSLRRYTLSGAASTMKIPRCPKHHRKLDLFCKSDGECICSECGDTEHQSHQIITAEKEWLNNKTQIDITETKIQEMIEQRLRKVEEIKFSLAEIKMAAEREKQDCIRVIGALLSSIERSQAELLEVTEMRRRSVEHQAESMLRELDLEVTELRKRSAALAKLAQTDCCISGLRSYSDINAQLPDKDWTGVSVKCNFETKSLYNSVCQLLIHSCQELQKLPEIYLSPLTEQSLLKAHPIDVKTIQEYAEDVILDPNTAHPRLILSEDRKEAWCGEKQKHVPNNPERFNRVVGVLAQQGFTGGQHYWEVEVSGKTDWDLGVASHSVNRKGKITFSPSNGFWLLCLRDNVDYTLRTEPSTTLPLNLKPQKIGIFLDYDKGQVSFYNVEAKMLIYTFMDTFSDAIYPFFSPCTNKSGKNDVPLTITPVLITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CU72</accession>
    <name>A0A3B4CU72_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL2m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L2, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537923.1">
        <property type="nucleotide sequence ID" value="XM_017682434.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537924.1">
        <property type="nucleotide sequence ID" value="XM_017682435.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CU72"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015647"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023886.2">
        <property type="protein sequence ID" value="ENSPNAP00000015647.1"/>
        <property type="gene ID" value="ENSPNAG00000021714.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411059"/>
    <dbReference type="CTD" id="51069"/>
    <dbReference type="GeneTree" id="ENSGT00940000153244"/>
    <dbReference type="OMA" id="EHNKEHV"/>
    <dbReference type="OrthoDB" id="268576at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000025">
        <property type="entry name" value="39S ribosomal protein L2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000157">
        <property type="entry name" value="39S ribosomal protein L2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF73">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="10">
        <location>
            <begin position="83"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="9">
        <location>
            <begin position="176"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="305" mass="33071" checksum="9BE8D480375CA24D" modified="2018-12-05" version="1">MALSSLCGALRALTVGSISTRTAAPVLSSSVLNSALLSVRPVNSSLPLCRGFLTTAALGQNRTLWKQTDKYTIRPIGVKKTGGRDFTGKIRRHGIGGGHKQRYRMIDFQRLRYEPGKEDQQIVEKVVEVRYDPCRSADIALVAGGNRKRWIIATENMQTGDLIKTSSAIGRMAVLANEGDAHPLGALPVGTLINNLELQPGKGAQYIRAAGTCGILLRKVNGTAIVQLPSKHQIQVLETCMATVGKVSNIDHNKRIIGKAGRNRWLGIRPSSGQWKRKGGWAGRKIRPLPPMKSFVNPPSLTAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CUF4</accession>
    <name>A0A3B4CUF4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574263.1">
        <property type="nucleotide sequence ID" value="XM_017718774.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CUF4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014973"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037535.2">
        <property type="protein sequence ID" value="ENSPNAP00000014973.1"/>
        <property type="gene ID" value="ENSPNAG00000020977.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440060"/>
    <dbReference type="CTD" id="768298"/>
    <dbReference type="GeneTree" id="ENSGT00940000162502"/>
    <dbReference type="OMA" id="LPTTWGM"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF4">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="67"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="2">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="528" mass="57117" checksum="549A3571AC11C1D5" modified="2018-12-05" version="1">MGLTVGERIQKWLLRAVSGFFFAAFRLFSRPEPVRPAKVPPVRNPLLTLPAVQLAEKIRRREVTSVEVVQAYIDRIQEVNTLLNAVIKDRFSAALSEAAHVDRLIDEETGGEEALADRLPLLGVPLSVKESFFLQGMPCSAGLVSRAGVLASGDAPAVALLKRAGAIPVGVTNTPELCMWVESSNYLYGITKNPYDTQRICGGSSGGEGSILGSGGSVIGIGSDIGGSIRMPSFFNGIFGHKGTTGIVSNDGQYPPSSARQQEFLSTGPMCRYAEDLLPMLRIMAGANADKLSLSSDVDLKKLRFFTVVDDGGSPLTSPVDKQLVEAQRKVAQRLEADLGVKVKEISFPQLKYSYQIWDTFLALPDEDGKPPQPFVELMADGGPAVWPVWELIKWLFGKSDHTMAAISLAFLKSIHSATPSQFILQQKETLQRSMEEVLGTDGVLLYPPHPVLAQEHHHPLFTPFNAAYTAIFNILGLPVTQCPLGLSVEGLPLGVQVVAGKLQDRLTLAVALYLQKAFGGWRDPGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWG3</accession>
    <name>A0A3B4CWG3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWG3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037495.2">
        <property type="protein sequence ID" value="ENSPNAP00000014874.2"/>
        <property type="gene ID" value="ENSPNAG00000032625.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165100"/>
    <dbReference type="OMA" id="VEAMAFF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014874.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="104" mass="11445" checksum="A5FDCE030C790F96" modified="2024-11-27" version="2">MNPAVEVMAFFLGFSGWVMSGISIPNKFWRVAEMEGNVITAHTVYENLWMSCMVDSTGVHNCQEFPSMLGLSGTLHSPATLLGTHIRSTTDRSLDVSSFSDRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWU1</accession>
    <name>A0A3B4CWU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hyaluronan synthase 2</fullName>
            <ecNumber evidence="9">2.4.1.212</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Hyaluronate synthase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Hyaluronic acid synthase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">HAS2</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-[hyaluronan](n) + UDP-alpha-D-glucuronate = [hyaluronan](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12528"/>
            <dbReference type="Rhea" id="RHEA-COMP:12585"/>
            <dbReference type="Rhea" id="RHEA-COMP:12587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132153"/>
            <dbReference type="ChEBI" id="CHEBI:132154"/>
            <dbReference type="EC" id="2.4.1.212"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:12529"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>[hyaluronan](n) + UDP-N-acetyl-alpha-D-glucosamine = N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-[hyaluronan](n) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20465"/>
            <dbReference type="Rhea" id="RHEA-COMP:12583"/>
            <dbReference type="Rhea" id="RHEA-COMP:12585"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132153"/>
            <dbReference type="ChEBI" id="CHEBI:132154"/>
            <dbReference type="EC" id="2.4.1.212"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:20466"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycan biosynthesis; hyaluronan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NodC/HAS family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.212" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWU1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015830"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024139.2">
        <property type="protein sequence ID" value="ENSPNAP00000015830.2"/>
        <property type="gene ID" value="ENSPNAG00000021924.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010337"/>
    <dbReference type="OMA" id="KSATYVW"/>
    <dbReference type="UniPathway" id="UPA00341"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
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    <keyword evidence="13" id="KW-0808">Transferase</keyword>
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            <end position="46"/>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="537" mass="61641" checksum="D961DB0C6CA21581" modified="2024-11-27" version="2">LSGQSACFSMRCEKLITYLRIIGTTLFGVSLLVGISTAYIMGYQFFTTTGHYLSFGLYGAILVVHLIIQSLFALLEHRNMRRSLETPIKLNKSLALCIAAYQEDPNYLRKCLISVKRLTYPGIKVIMVATYVWKSNYHQRGPEETEESYGESLQQVSRLVLNNKCVCIMQKWGGKREVMYTAFKALGRSVDYVQVCDSDTMLDPASSVEMVKVLEEDPMVGGVGGDVQILNKYESWISFLSSVRYWMAFNIERACQSYFGCVQCISGPLGMYRNSLLHEFLEDWYNQTFMGSHCSFGDDRHLTNRVLSLGYATKYTARSKCLTETPITYLRWLNQQTRWSKSYFREWLYNSMWFHKHHLWMTYEAVITGFFPFFLIATAIQLFYQGRIWNILLFLLIVQAVALIKSSFASCLRGNVVMVFMSFYSVLYMSSLLPAKMFAIATINKSGWGTSGRKTIVVNFIGLIPISVWFTILFVGILYTIVQDTRKPFGESEKIVLIIGAIVYASYWVMLLTLYVVLIAKCGKRKKEQQYDMVLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CX63</accession>
    <name>A0A3B4CX63_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Metabotropic glutamate receptor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GRM3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with TAMALIN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580757.1">
        <property type="nucleotide sequence ID" value="XM_017725268.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CX63"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015923"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024277.2">
        <property type="protein sequence ID" value="ENSPNAP00000015923.1"/>
        <property type="gene ID" value="ENSPNAG00000022025.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444204"/>
    <dbReference type="CTD" id="2913"/>
    <dbReference type="GeneTree" id="ENSGT01030000234595"/>
    <dbReference type="OrthoDB" id="425344at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008066">
        <property type="term" value="F:glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007216">
        <property type="term" value="P:G protein-coupled glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15284">
        <property type="entry name" value="7tmC_mGluR_group2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06375">
        <property type="entry name" value="PBP1_mGluR_groupII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000001">
        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metabotropic glutamate receptor 3"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001234">
        <property type="entry name" value="GPCR_3_mGluR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000162">
        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
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        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
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        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01053">
        <property type="entry name" value="MTABOTROPC3R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017464713" description="Metabotropic glutamate receptor 3" evidence="17">
        <location>
            <begin position="23"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="695"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="743"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="774"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="802"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="18">
        <location>
            <begin position="581"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="887" mass="98380" checksum="66862A23DE0D0112" modified="2018-12-05" version="1">MSSAVRALLLVLLGAGVLLSSGDSPRREIKIDGDLVLGGLFPIHEKGLGMDECGRINEDRGIQRLEAMLFAIDQINRDVSLLPGVALGVHILDTCSRDTYALEQALEFVRASLTKVDDNEFICPDGSYAFQEDSPLAIAGVIGGSYSSVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFYQAKAMAEILRAFNWTYVSTVASEGDYGETGIEAFEQEARMRNICIATSEKVGRSNAKRSSYEAVVRQLLQKPTARVVVLFLRSDDARELLGAATRLNASFLWVASDGWGAQESIVKGSEFAADGAITLELAAHPILEFNRYFQSLNPLNNHRNPWYKDFWEQKFQCSLGGTSSSAGLGTPKPPCDPGLAVEESNFEPESKIMFVVNAVYAMAHALHRMQRTLCANTTRLCDAMRSLDGRRLYRDFLLHISFRAPFSPPGSENVVKFDQYGDGMGRYNIFNYQRLPGSDRYTYVQVGEWAETLSLNEFLIGWPRGGMMPTSQCSDPCAPNEMKKMQAGEYCCWICTACEAHEYLPDEFTCAPCSLGQWPRADLSGCYDLPEDYIMWEDAWAIGPISIACVGFVCTSLVFIVFIRHNDTPLVKASGRELCYILLLGVLMSYTMTFVFIAKPSPAVCALRRLGLGTSFAVCYSALLTKTNRIARIFSGVKEGGAQRPRFISPSSQVFICLSLISVQLLLVSVWLLLEVPGTRRFTLPEKRQTVILKCNVRDSSMLLSLSYDVVLVVLCTVYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCMFAPKVHIIMFQPQKNVTSHRLNLNRFSVSGPATTYASHASVGAHYVPTVCNGREIVDSTTSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CXX8</accession>
    <name>A0A3B4CXX8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Splicing factor 3A subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SF3A1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins. Part of the SF3A subcomplex of the 17S U2 SnRNP complex which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62 and SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the mature 17S U2 small nuclear ribonucleoprotein complex (17S U2 snRNP). SF3A1 functions as a scaffold that interacts directly with both SF3A2 and SF3A3. Identified in the spliceosome 'E' complex, a precursor of the spliceosome 'A' complex. Identified in the spliceosome 'A' and 'B' complexes. Identified in the spliceosome 'C' complex. Interacts with P2RX6; resulting in a reduction of the splicing activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017539492.1">
        <property type="nucleotide sequence ID" value="XM_017684003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CXX8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016215"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024640.2">
        <property type="protein sequence ID" value="ENSPNAP00000016215.1"/>
        <property type="gene ID" value="ENSPNAG00000003721.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412103"/>
    <dbReference type="CTD" id="10291"/>
    <dbReference type="GeneTree" id="ENSGT00730000111077"/>
    <dbReference type="OrthoDB" id="447637at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01800">
        <property type="entry name" value="Ubl_SF3a120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000002">
        <property type="entry name" value="Splicing factor 3A subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000091">
        <property type="entry name" value="Splicing factor 3A subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000001">
        <property type="entry name" value="Splicing factor 3a, subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045146">
        <property type="entry name" value="SF3A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022030">
        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035563">
        <property type="entry name" value="SF3As1_ubi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SURP motif" evidence="16">
        <location>
            <begin position="40"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="16">
        <location>
            <begin position="155"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="15">
        <location>
            <begin position="699"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="306"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="526"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="649"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="649"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="785" mass="88735" checksum="745E8EB51AC6B2B8" modified="2018-12-05" version="1">MPPVQISQPEPNKADEQKEETPATKPIVGIIYPPPEVRNIVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPTDPYHAYYRHKVNEFKEGKGQEPSAAVPKVMQQQSMQQSQQQLPQKVQAQVIHETIVPKEPPPEFEFIADPPSISAFDLDVVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYFTKLVEQYTKVLIPPKGLLQKLKREAENPREVLDQVRYRVEWAKFQERERKKEEEEREKERVAYAQIDWHDFVVVETVDFQPNEQGNFPPPTTPEELGARILIQERYEKYGESEEVEMEVESEDEEDERENRDEGHAAQPDQDTQLQDMDEGSDEEEDGMKAPLPPDNPMPPPLPPTPDQVIIRKDYDPKASKPHPTIQATEEYLISPITGEKIPASKMQEHMRIGLLDPRWLEQRDRGIRERQIEEEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVQEDDTKEKIGPSKPNELPQPPLPSTVTSIPKPSPPINTMPKPPPAVPPPVPTTLLSAVPVIPRPPMAPVVRLAPGQVLTPMPPMVHAPRINVVPMPPTAPHIMAPRPPPMVVPAAFVPAPPVPQPPSAAPAPMPPAHPPPPHEDEPISKKMKTEDNLIPEEEFLRRNKGPVAVKVQVPNMQDKTEWKLSGQVLSFTLPLTDQVSVIKVKIHEATGMPAGKQKLQFEGIFIKDSNSLAYYNMNNGSVIHLALKERGGRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ16</accession>
    <name>A0A3B4CZ16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554660.1">
        <property type="nucleotide sequence ID" value="XM_017699171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ16"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015794"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024080.2">
        <property type="protein sequence ID" value="ENSPNAP00000015794.1"/>
        <property type="gene ID" value="ENSPNAG00000021846.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428287"/>
    <dbReference type="CTD" id="100192217"/>
    <dbReference type="GeneTree" id="ENSGT00990000204320"/>
    <dbReference type="OrthoDB" id="8870994at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:defense response to bacterium"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000012">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF190">
        <property type="entry name" value="C-C MOTIF CHEMOKINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0145">Chemotaxis</keyword>
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    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="60"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="135" mass="15614" checksum="901B4B065FBD3FC8" modified="2018-12-05" version="1">MRLKKRNQAVHKSLSLNQAQHFRRDGTLQTALSFIQRLSQGCQVPIRRTPPPTLADHSRIKSCCRKYTKGEIPFTRIRGYSIQTVRTFCNIDAIIFHTSIGRNVCADPSQNWVMETIHKLKDKVRAMTKKQAKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ30</accession>
    <name>A0A3B4CZ30_PYGNA</name>
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        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ODF3B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSPNAT00000025102.2">
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        <property type="gene ID" value="ENSPNAG00000003733.2"/>
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    <dbReference type="OMA" id="SKHYLTW"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="entry name" value="Si:dkey-234i14.13"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IGv"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="11"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016573.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="194" mass="21058" checksum="2260CEFDCBA21BEF" modified="2024-11-27" version="2">MDRRHVALSAPCFSALGEFIVTVTQTPAVKSALPGDTVTISCRTSSAVYHHSSNGHYLHWYQQKPGETPKLLIKLANQLQSGFPARFSGSGSGSDFTLTISGVQPEDTGDYYCQSYHYINTIQKPPSVRRYRPCTNAVGPTAGAGVGVATQCRTHSSSISPTDSQKTRLQTVKQCFRGMNSLLSSLPFTLIDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZW6</accession>
    <name>A0A3B4CZW6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000016119"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038004.2">
        <property type="protein sequence ID" value="ENSPNAP00000016119.2"/>
        <property type="gene ID" value="ENSPNAG00000022242.2"/>
    </dbReference>
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    <dbReference type="OMA" id="TECCELF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016119.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="172" mass="19224" checksum="7080BB4EA2D552B6" modified="2024-11-27" version="2">MAEDHGLCDGDCPIDVTECRKLVLFIIAEHIILLNGVQGLLLPLQFDDVGIWNDPLSELPHRVLKGRKLLHMPCSPLNADALVLMALCGYHNVSLVQHEHSNLLGHSARSTNNYLLLDLAASKLLTFIATNSICQFHFRVEPPHLLHHFACLQGELIGWRNAQTLERRNTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2V2</accession>
    <name>A0A3B4D2V2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thrombospondin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2V2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017843"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026793.2">
        <property type="protein sequence ID" value="ENSPNAP00000017843.2"/>
        <property type="gene ID" value="ENSPNAG00000006656.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168119"/>
    <dbReference type="OMA" id="HRGYTSI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF966">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXII) CHAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thrombospondin-like N-terminal" evidence="4">
        <location>
            <begin position="16"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="294"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="512"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="604"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="724"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="642"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="697"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="730"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="741"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="769"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017843.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="780" mass="81637" checksum="E0F1603A9C1DB6FF" modified="2024-11-27" version="2">YRNSMNLLAVLNVLGCYDLLEEFRLPESRGVRRVEGSEPDAVAYRVNPSLHLRKSISDAYPDGLPADYSIIATFKITKDAAKQSWNLWQVSDPEGREQVGLRFHGDSRTLDFFYSGPGGTQMLRTFHGVEKLFDGEWHKLALSVKGDQARLLVDCEEVSAEPLDEPRPLIRRGYTSIAKRAVGDRSVSVDLQKMHVSCDADQAYSEGCCELSNVCGGYAEIGLTAGRGRATCKCQHGQPGPQGRAGQKGHRGLAGNTGDVGRLGNWVRNLIEISHLHVGFHHGEAGIKGEKGMRGLHGQEGDRGPKGLKGLPGAAGHKGVRGPPGERGETGAPGEKGLSGDRGYRGIPGYQGVKGEEGFSGAEGPLGPRGLQGIVGDPGERGKLGEKGKPVHISPGIVGDPGLPGRDGDPGIEAYQGLQGSDGRSGRAGERGEKGDQGPPGNMGPQGRTGLQGYKGSAGKPGRPGFIGPPGRTGHVGSPGRPGAKTNVTFRVDLILYILYNTDICVWKAGDRGKPGPQGSQGKRGPAGEHGRKGRVGTKGVRGDGGPVGFQGPPGPPGPTLAAQHVIEVCKKVVLEQMSTFANSVKRTCAAVCPLYGEVPMGAPGPAGPKGPPGPPGDPGTDGADGELGQQGFYGEAGDPGRQGERGDPGERGDRGPKGYGLPGHIGDQGKKGQSGRPGRAFDGRPGQMGERGHVGRPGLRGHPGLRGVPGVCLTSGCALLNATAKGAPPQAPQWAPEPAPQRSSQRGSQRAPQRAPQRPSQRPSERSFQWTSQRQRTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D579</accession>
    <name>A0A3B4D579_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000027084.2">
        <property type="protein sequence ID" value="ENSPNAP00000018064.1"/>
        <property type="gene ID" value="ENSPNAG00000006475.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157047"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13184">
        <property type="entry name" value="FERM_C_4_1_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000002">
        <property type="entry name" value="Erythrocyte protein band 4.1-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008379">
        <property type="entry name" value="Band_4.1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007477">
        <property type="entry name" value="SAB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF20">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05902">
        <property type="entry name" value="4_1_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04382">
        <property type="entry name" value="SAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="7">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="791"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1229"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1360"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="820"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="840"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="856"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="901"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1253"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1370"/>
            <end position="1391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1532" mass="171514" checksum="FB07C0503455380E" modified="2018-12-05" version="1">MQCRITLLDGSDYTCTVEKKAKGQVLFDKVCDHLNLLEKDYFGLTYRDAENQKNWLDSAKELKKQIRTGPWNFGFNVKFYPPDPAQLCEDITRYYLCLQLRDDVVSGRLPCSFATHSLLGSYIAQSEIGDCDPEELGSDYVSDLRFAPNQTRELEEKVMDLHRSYKGMTPAEAEMLFLENAKKLSMYGVDLHHAKLVGRLFECLAAAKEEDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKILKISYKRNNFYIKIRPGEFEQFDSTIGFKLPNHKAAKRLWKVCVEHHTFFRLVSPEAPPKKVLSLGSKFRYSGRTQAQTRQASCQISRPAPQFQRSSSKRHTTSRSLDGAPAMANNDNLMKDSKPTGTHITTITPEKKIEEEVAVEEETTTTAETSEPAASTPVVKNSFIRRIKGDNVFVKHSNLMLEVTDAPEELLKRQMNLNELKRSFLETRPEWSGDSEWDRRLSSSPARALRRNDTPIIEPVLILQDTAEDVKAEPPQDISEEKPELLEASKTEVSEQNMHIICNMTKVGNAPCDLEAMNETTKLAPLMEVNSAATETDSVTVSNHYHKLCDNPVLEKACSVLSGEANADNTSEALNITMQESNFFEDKLNCMVVKINMPDSGIAKVMSATTRPQQKIAEQRETLTSSIQKMTDSNKDEAAFEGIIEHVDYSVKTLLILNPEKTVVQTAAPQKPMLRGVEWNMPHLEMACRNQLQSRRVSVHYITKTSPTFRVVSKMAYISEFPQQCVEVLPEKDTRSVKYTFSYLLQSAGDQQEDTPIVEEVLEGEEAEETDKKEPLTDEAQEEKSLPCTVQKEKSPPYEPEERSASAPCETQEKSPHCDTEKKSLAPNETQEETSAPNEADEEKSPLYEAQEMTSPLYDSEEDKPHYEAEGESSTLYETQEQTSFTCEVEEENSPPYEPQEELSATTLNIRTPADAINEDSSGGQIASPASLCLTEEKESPVNGLQQAKEEIQLSELGNCAAIVQSAPWCTESYTAEQVPQDVVCFVMAEQLPLDTQFRNCAQVEAQEEVGEQTIASESMPSALVTESAVDWTHTEKETLTPSLTEECRDQEISESAAMVPSSGETQMYMVEVALLQAIPGLADVAPLLSAYNLIEEEGDVLDQTTIENKAEKIEEQAEGERKLSEIGSMKEAEGAVQMEAFVSSRDMDTLEPPAAQLSDQVEKIPKEMQERTTHVKEDSNEREESYLEECVEQVYENPLTPNIDEGHVFPENSQEYEEEKEQTASSGSSTPIRKSVCEDDESFVYEEGENSWEISIDYATSAIEEQEQANVNTHDESYSSRLGNCSASVQFVQVQEPIIEEQLPLEIQIEEDSAEGFGNFTSPETCKLACTNKEGEDNDSERKENNDKEGEVVPQKIEKRKEEMNTFSEAVIPFLEESEEPVQAVMTTEVPVVHTETKTITYESAEVDANGDTDPGVLMSAQTITSENNSTTTTTHITKTVKGGISETRIEKRIVITGDANIDHDQALAQAIKEAKEQHPDMSVTKVVVHKESEISLGEGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAL9</accession>
    <name>A0A3B4DAL9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017546619.2">
        <property type="nucleotide sequence ID" value="XM_017691130.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DAL9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020024"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039783.2">
        <property type="protein sequence ID" value="ENSPNAP00000020024.2"/>
        <property type="gene ID" value="ENSPNAG00000004118.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423669"/>
    <dbReference type="GeneTree" id="ENSGT00940000158953"/>
    <dbReference type="OMA" id="YIGHYLF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000781">
        <property type="term" value="P:positive regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990166">
        <property type="term" value="P:protein localization to site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17738">
        <property type="entry name" value="BRCT_TopBP1_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042479">
        <property type="entry name" value="Slf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057595">
        <property type="entry name" value="TopB1_SLF1_BRCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46677">
        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46677:SF1">
        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23294">
        <property type="entry name" value="BRCT_TopB1_SLF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="11">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="635"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="669"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="595"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="597"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020024.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="891" mass="99181" checksum="7A672ED963F7445A" modified="2024-11-27" version="2">MVGNKYVFQMTGVRNLQQKRELLQGIRRLRGKYIGGSEYQETMTHLIVTRLVASEKFIAACAGGKWIVTPEFVLDSVKQQAWLSEASYELNLSANLKALAITNPLQKWREKVARGFVSGAFQGWVVRLDIEDTGKRGMFKRILKAGKAVVCTDKSATQAVTHVFTKDGSKMSQNVRAPYHTISFIAHHLFGSLCSSLNWSINLDKPKFQENFVQEEATEDSSLKMEEESMDVMYNLLLCELEEKLKDYIMKMELRKRKLLKVPELFNYYTPVFPTQVATVDFSNVCSLVECGLFPQAMEDVQCSLQPGVLPPASALHACMQHALHGEAQSYFLSMFSTVLNDILRNNPTWRSPGSVKYFLQILQCPQCKNGVWPLLQTSVRFCLSSAATCHSLPSPAPSELLRFHGNLQAFFLRLFQLELRAAISCGGGSRTSVLYSMFWNVWEKSILSSRALQQLAELLVEATLWGLHSTQEWKGRLLATLQEILAVVVEYWAQEHSKLNSRLVDKGFQDLAEYIAILCQDLHPDTLKELVPALPSPRLRMLTVEAIYRNICSRNGLFIHPEPLSLLKIVSSYLKALGQLCGCSLMHAHNRRAERSISTGSDSQVSNTGEKDRVGGSGSGKENIPRGFHRVNPAGETLLHRACKRNQVETVLHILSLPGTDVNVKDHAGWTPLHEACNHGSSECVEVLLQNCPGLQLDSQVKGVSPLHDALLNQHTDIAKMLLRHAGSSLLQLRDDFGCTPLDLVSSALREELQRCAQEADSALEVQCSEVRDLAFIEGCSCLLSCLLLSYLLEHHIPSYEAPDPPLELSPSIAQALITTSPETVASSWGDSRAVSLAEDLRTLMSMEQYVLQVNPALMHCQGPHTSVFVQLLKDLQADGVALLNGQPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAY9</accession>
    <name>A0A3B4DAY9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NSL1 component of MIS12 kinetochore complex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">NSL1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017556480.2">
        <property type="nucleotide sequence ID" value="XM_017700991.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DAY9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021527"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040618.2">
        <property type="protein sequence ID" value="ENSPNAP00000021527.2"/>
        <property type="gene ID" value="ENSPNAG00000004391.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429321"/>
    <dbReference type="GeneTree" id="ENSGT00390000001374"/>
    <dbReference type="OMA" id="AWQETSD"/>
    <dbReference type="OrthoDB" id="5973266at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013950">
        <property type="entry name" value="Mis14/Nsl1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31749">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN NSL1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31749:SF3">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN NSL1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08641">
        <property type="entry name" value="Mis14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021527.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="30367" checksum="7D3A8EF323E70F7F" modified="2024-11-27" version="2">MQRRSFRVYFGGTHRFEGLAVEMEDAEKEPDYRVNVKSKKVVLQQIARYKSLLKKLLDGQSQLCEDDKEKLCQEMLTNFEIVVQENIIVDGLPWEEAAEEDGDDCEFGALDDMLDEKIVQTTRKRSIYPKKILPYVVRSLKAERKLMGLFENTVKPQEVKRDPAQDTFMSKVSAAAPEMFSQASLVIKSLKTLKQTAEGLHQVLNMQPSAETVEVYRDVFGSSNGEGCPGLCNKAPVRRQTIRRAASETEYSMDYIPLAKIPATSAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBJ1</accession>
    <name>A0A3B4DBJ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hyaluronidase</fullName>
            <ecNumber evidence="10">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SPAM1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 10">Belongs to the glycosyl hydrolase 56 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBJ1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031689.2">
        <property type="protein sequence ID" value="ENSPNAP00000020840.2"/>
        <property type="gene ID" value="ENSPNAG00000006331.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230364"/>
    <dbReference type="OMA" id="NRRWGFY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030214">
        <property type="term" value="P:hyaluronan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000065">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018155">
        <property type="entry name" value="Hyaluronidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769">
        <property type="entry name" value="HYALURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769:SF20">
        <property type="entry name" value="HYALURONIDASE PH-20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01630">
        <property type="entry name" value="Glyco_hydro_56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038193">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00846">
        <property type="entry name" value="GLHYDRLASE56"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="8">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="45"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="365"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="420"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU610713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020840.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="443" mass="49812" checksum="6EBC23DDCC95977E" modified="2024-11-27" version="2">LPRGRPRTCWRDYFSNTASSLPFTAAPLFTDRPFAVIWNAPTHVCKNLNISLDFSVFQAVTTTTGEPDQFLFLFYTNKLGLYPYIDPSTKKEYNGGIPQKGNLTAHLEKAWRDITQYLPEFNPGLSVIDWEAWRPLWARNWDSKDIYRKQSIQYAQAKDPSLSAANAVTVAKEEFEKAAKSYMMKTMNLGIRQRPKYLWGYYLFPNCYNYGWNETGYTGECPALEIARNNELLWLWESSTAFFPSAYLPLSLKESHKGALFIRGVVKEAVRVSALPNLVYTSPIYVYLRPLFRDQNKEYLSEADLVRTIGESAALGASGAVLWGATADYNNKPSCEALAAYLPSTLNPYVVNVTAAAKLCSDTLCQSNGRCVRKNPNSDDYLHLHPDHFQIQAGNGKRLAVGVPSLSDLKFFADKFTCQCYVGKDCSASLSFSPPQTAVIIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DG48</accession>
    <name>A0A3B4DG48_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Interleukin-1 receptor accessory protein-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">May regulate secretion and presynaptic differentiation through inhibition of the activity of N-type voltage-gated calcium channel. During presynaptic differentiation may regulate both synaptic vesicle accumulation in axon terminals and subsequent axon terminal remodeling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017562061.1">
        <property type="nucleotide sequence ID" value="XM_017706572.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DG48"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023382"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012785.2">
        <property type="protein sequence ID" value="ENSPNAP00000023382.1"/>
        <property type="gene ID" value="ENSPNAG00000007939.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432614"/>
    <dbReference type="CTD" id="568868"/>
    <dbReference type="GeneTree" id="ENSGT01150000286976"/>
    <dbReference type="OMA" id="WQWRETI"/>
    <dbReference type="OrthoDB" id="9925886at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045920">
        <property type="term" value="P:negative regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000188">
        <property type="entry name" value="Interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000284">
        <property type="entry name" value="interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000220">
        <property type="entry name" value="X-linked interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000009">
        <property type="entry name" value="X-linked interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF22">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017391374" description="Interleukin-1 receptor accessory protein-like 1" evidence="20">
        <location>
            <begin position="20"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="160"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="245"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="21">
        <location>
            <begin position="407"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="681"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="25">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="721" mass="81248" checksum="B97CC56FC5621302" modified="2018-12-05" version="1">MTALIPMLLLLTGLSVSFTLKVVSKRGSVDGCTDWSVDYLKYRVLHGEPVRIKCALFYGYIRANYSQAQSGGLSLMWYRSSGLGHGDFEEPITFDGLRMSKEEDAIWFRPAELQDAGLYSCVLRNSTFCMKVSMTLLVADNDTAACYNTKLRYSEKAELGKSKDITCPDIQDYIQPGQTPQITWYKECGVWQWRESILQSADTLSIQEVTEDDIGNYTCELPFGGFVVRRTTYLSVTAPLTEEPPKILFPSENKLLTLDLQIGSAVNLTCRAFFGYSGDVSPLVYWMKGEKFIEDMDQSRIRESDIRTIREHLGEQEMSITLTIDSLEEGDLGNYSCYAENGNGRRQASIQISKRVELMYTVELAGGLGAILLLLVLLLCVYKCYRIELLLCYRHHFGGEDTDGDHKEYDAYLSYSKVELDQWGQELQEEERFALEILPDVLEKHYGYKLFIPDRDLIPTSSLSSEHYQDDTQLLRAYIEDVARCVEMSKRLIIVLTPGYVLRRGWSIFEMESRLRGSLASGEVKVILIECAELRGLINYQEVEELKQSIKALTVVRWGGPPSNKPGSHFWKRLRYAMPCRRPPLHRTLANPAADGGEPGPFADLQAVSAVSVATAAVAPARPELRTALRSSYRSHSLARQKHAHFRSYDYELPLAAGATLPTPSQQHTYCNLPLTLLNGQRPTAAGPKGLRQHSLDEPHANNNAMLPLLPRETSISSVIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJQ7</accession>
    <name>A0A3B4DJQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560254.1">
        <property type="nucleotide sequence ID" value="XM_017704765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJQ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023169"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013332.2">
        <property type="protein sequence ID" value="ENSPNAP00000023169.1"/>
        <property type="gene ID" value="ENSPNAG00000007701.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431537"/>
    <dbReference type="CTD" id="30072"/>
    <dbReference type="GeneTree" id="ENSGT00940000157116"/>
    <dbReference type="OMA" id="CCKKHFY"/>
    <dbReference type="OrthoDB" id="6516235at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031100">
        <property type="term" value="P:animal organ regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110021">
        <property type="term" value="P:cardiac muscle myoblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19404">
        <property type="entry name" value="TGF_beta_INHBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000005">
        <property type="entry name" value="Inhibin beta A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF133">
        <property type="entry name" value="INHIBIN BETA A CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00669">
        <property type="entry name" value="INHIBINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017301869" description="TGF-beta family profile domain-containing protein" evidence="9">
        <location>
            <begin position="41"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="10">
        <location>
            <begin position="293"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="414" mass="45585" checksum="04D076300B1C904D" modified="2018-12-05" version="1">MLVSQTVRLVCITPPESAAHSPRMTLLPALSGALLLLVHCCSISAMATSGSLPSDCPSCALSRLRRGGPDALVEEAEAQQDVVEAVKRHILSMLHLQARPNITHPVPRAALLNAIRKVHVGRVAQDGSVLIDDEPHSRQEAESAEQTEIITFAESGEAPGLINFLISKEGDEMSLVEQANVWLFLRLPKGNRTRTNVTIRLLLQLPGSGEKLVAEKAVDTRRSGWHTFPVAASVQSLLEQGGSSALKLRVSCPLCADARATPILVNPGGGEREQSHRPFLMAVVRKVEEQGPRRRRKRGLECDGKVRVCCKRQFYVNFKDIGWNDWIIAPSGYHANYCEGECPSHIASITGTSLSFHSTVINHYRMRGYSPFTSIKSCCVPTRLRAMSMLYYNEEQKIVKKDIQNMIVEECGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DK55</accession>
    <name>A0A3B4DK55_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 710</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DK55"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023314"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012957.2">
        <property type="protein sequence ID" value="ENSPNAP00000023314.1"/>
        <property type="gene ID" value="ENSPNAG00000000514.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444484"/>
    <dbReference type="GeneTree" id="ENSGT00940000161170"/>
    <dbReference type="OrthoDB" id="7295497at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000161">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000182">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000186">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000191">
        <property type="entry name" value="zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000203">
        <property type="entry name" value="Zinc finger protein 366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000451">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000502">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF243">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="465"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="559"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="587"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="615"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="124"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="198"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="706" mass="79513" checksum="E47392990B0B3AEF" modified="2018-12-05" version="1">MRSLKHLKPQTKKNVEEKTQRLVRCYSEAMHQQMDVGTQTDPVVVLSLAQAAVLGLISQNEVFGATIAPNGFYTGDPKESPAPPSERMDYEYAEQLIGANGDYLGVNLGEDNHMHPSCAERRWQGPSENGKTPTGHLDAGSHVKGESVTPGLPSCNHMMNSMIPRESMQMMDPSGYRVAPKPHANNCCATCVREIKSTHPPSDHHTHSHPHSHPHAAHEVPPRDRNHTQEKGGVDEVPEEGDDGRMGKAGGGGEEISSYFQASEVGYEGGDMDGVGDFDESSQGMFWGEHVDGEAPRGRRIDRLDINIQINESYCVDVGEGLKRWKCRMCEKSYTSKYNLVTHILGHNGIKPHACPHCGKLFKQPSHLQTHLLTHQGTRPHKCTVCKKGFTQTSHLKRHMLQHSDVKPYSCRFCRRGFAYPSELRAHEVKHERGRCHVCSHCAMEFPTYAHLKRHQVSHQGPATFQCGQCSKAFAYRSQLQNHMLKHQTQRTFSCSQCGLQFLQLHQLRQHSLTHKVLKPQARASKGMKGFKCDVCAREFTLSANLKRHMLIHASVRPFQCHVCFKSFVQKQTLKTHMIVHLPVKPFKCKVCGKSFNRMYNLLGHMHLHAGSKPFKCAYCSSRFNLKGNLSRHMKVKHGMDVSPDGQDAPPDLEPHEDYEEENFNFATPEHMDNNDAPNLTKLSEVAIQELDYYNFGKDVSSYNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKT7</accession>
    <name>A0A3B4DKT7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017564007.1">
        <property type="nucleotide sequence ID" value="XM_017708518.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKT7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008730.2">
        <property type="protein sequence ID" value="ENSPNAP00000024992.1"/>
        <property type="gene ID" value="ENSPNAG00000009848.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433751"/>
    <dbReference type="GeneTree" id="ENSGT01100000263479"/>
    <dbReference type="OMA" id="LTCQTAH"/>
    <dbReference type="OrthoDB" id="6353782at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052598">
        <property type="entry name" value="IgSF_CEA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337:SF17">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="127"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="222"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="326"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="404"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="532" mass="57441" checksum="F75A3156E80A1B4C" modified="2018-12-05" version="1">MDLCSLRAPLLILAAIICCSYSLLVPLKISGVKGKYAIFPLKITDGFQPNAVKITFTNSAGEFTTVYNRVGEKVTTSSNGRAVYNQTTYEFQLGPLTPGDAGQYSFILVDTEYNMNEGQTTLEVLEPVADVVIVSSLPEAVEFNSTVVLTCSAKGSFLSYKWLNGSTTPVVVDGKHLVLNGSQLIITEVLRTDLRGSIYCVAENQLESVRSTAFNMSVSYGPESISMKKVPDEIDLKKGSNLTMSCSAVSSPAAELKWLFNGAELPDKSATLAYTNLEENQSGNYSCVAYNSKTKRYAASQVITVSIIEAISGTNVTGPISLLIAGNSTANLTCKSSMGKADSVTWFKDEKPLAASDRIIISTDKKTISIPIVQKEDAGQYKCKLSNKLSTDSSIYNMQIYYGPEKVSIEGPKKAEVVDTVVMTCKVTSIPPATYIWKFNGSLLEITTAEFKIEKPSFENSGIYTCEARNHITGLSQTATHNLLVKGEGELNDQLSSGAIAAIVIAVLVIVAIIIAVIIRKRRKPGDIASPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLJ0</accession>
    <name>A0A3B4DLJ0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine rich repeat containing 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LRRC31</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574722.1">
        <property type="nucleotide sequence ID" value="XM_017719233.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLJ0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023981"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011302.2">
        <property type="protein sequence ID" value="ENSPNAP00000023981.1"/>
        <property type="gene ID" value="ENSPNAG00000008448.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440393"/>
    <dbReference type="CTD" id="79782"/>
    <dbReference type="GeneTree" id="ENSGT00730000111293"/>
    <dbReference type="OMA" id="CVTEEDM"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042419">
        <property type="entry name" value="LRC31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24109">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24109:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="61990" checksum="408DD438F17270CF" modified="2018-12-05" version="1">MESDQQRGREASQKRSPLDLIMNQIRRKTSFTERKKPSMGRLFRPSVSSDKNGEIPETKEAESSEGKGSTGPGTESSDTDSEISSVVGWGRVKQFVQKLGKTPDSQSLSLAHCDLTATDVVELGTLLPFLAQLEVMDLSWNDLLGGSLKALTCHLQHVGKLKVLKVSSCRLTAQDLTALGEALDCVPLMEMLDLSWNAGVGGGNLHCLTKHLHSASSLRELHLVDCHLSEADAVALGEVLLLLTCLEVLDLSNNKLMVKGVEHVASSLNSTPQLKTLKLSMCGLNQDSLAILGEKLRFLPGLEHLDLSCNKQSGGGFSTMTTSLCLLTHLKCLDMHLCCLTEDDILELVQAVPSLGELTELDLGSNKSVGGVIRSLLPVLPLSKMKKLYLNNCGLSPETCQALATTMQSLRQLECLNLSWNKNVGEILQQLLEPLQADCKLEELRLSSCDLTTEDMLHLESACERGALSKLKLLDLSYNGSVGDGGWVSLFGAVAGLKELQELDVSLRPSASLSASPWLPALLDALPQLPSLRRLSLQRWALGAGEREKLEKSLTKRNVVLECDKAIEKAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DN56</accession>
    <name>A0A3B4DN56_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thromboxane A2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Prostanoid TP receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PTGIR</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552326.1">
        <property type="nucleotide sequence ID" value="XM_017696837.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DN56"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024903"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008956.2">
        <property type="protein sequence ID" value="ENSPNAP00000024903.1"/>
        <property type="gene ID" value="ENSPNAG00000009713.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426977"/>
    <dbReference type="CTD" id="5739"/>
    <dbReference type="GeneTree" id="ENSGT01050000244902"/>
    <dbReference type="OMA" id="IHPFCGD"/>
    <dbReference type="OrthoDB" id="5959154at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016501">
        <property type="term" value="F:prostacyclin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004960">
        <property type="term" value="F:thromboxane receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048662">
        <property type="term" value="P:negative regulation of smooth muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000175">
        <property type="entry name" value="Prostaglandin D2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001105">
        <property type="entry name" value="Thbox_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF7">
        <property type="entry name" value="PROSTACYCLIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00429">
        <property type="entry name" value="THROMBOXANER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="35"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="370" mass="41002" checksum="DCAA024D027FF169" modified="2018-12-05" version="1">MLHNNTCENITQILVEKNPGNLVVSAIMFLAGVVGNLLALAILGFRQKERRAKSSVFCILVTGLALTDLAGTCLLSPPVFVCYAHSTSLVGLTGDMRLCSLFAFAMTFFGLASMLILCAMAVERCLAISHPYFYSKHVRRRFAKVVLVLIYLFTCAFCLLPFFGFGRHKQYCPGTWCFIKMEAEGEGKEEEIQTLSFSLSYAVLMALLIAVVFMCNGSVIVSLCRMHRSQMTRRGSVVSAGRKRRVSSWFSQGEEEMDHLVLLACMTIIFVICSLPLTIFGFVNAVSPVGNDQQNLLAFRFSALNPVVDPWVFIIFRKSVFQSVRAFFCCHFRRAAVKATPHSGLSFPLQSTGPAKSAALQSKTYLNIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DND2</accession>
    <name>A0A3B4DND2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peripherin 2-like a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRPH2/ROM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555246.1">
        <property type="nucleotide sequence ID" value="XM_017699757.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DND2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024616"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009669.2">
        <property type="protein sequence ID" value="ENSPNAP00000024616.1"/>
        <property type="gene ID" value="ENSPNAG00000009244.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428636"/>
    <dbReference type="CTD" id="100331800"/>
    <dbReference type="GeneTree" id="ENSGT00940000163655"/>
    <dbReference type="OMA" id="LQIGFRC"/>
    <dbReference type="OrthoDB" id="9836210at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03162">
        <property type="entry name" value="peripherin_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000002">
        <property type="entry name" value="Retinal outer segment membrane protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000830">
        <property type="entry name" value="Peripherin/rom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042026">
        <property type="entry name" value="Peripherin_LEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF184">
        <property type="entry name" value="PERIPHERIN 2 LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00218">
        <property type="entry name" value="PERIPHERNRDS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41615" checksum="392754AC78A176FC" modified="2018-12-05" version="1">MAVLRVKFTKSQRDKLAQVLWLLNWVSVVTGIILFSLGLFLKVEINKRRELMAERAIHSVPNMLIAVGLIACIINFVGGKICYDCADTNKFLRWKLVMLPYIICTFFFTLCVLVGALMCYSMHGELEESLALGLSDAMRYYKDTDTPGRCFLKRTIDLLQIQFQCCGNKGHRDWFQIQWISNRYLDMSKQEVLDRLRSNVEGKYLMDGVPFSCCNVNSPRHCIQYQLTNNSAHFNYDYKTEDLNLWKSGCHQALVEYYTNIMHSIGLTVLLIWLFELSVLTGVRYLQTALENVLRQGDPESESDGWLLENSFVETARSNFNIIKNLGKCNQVDTTVNGDPNIDKPCTAHYGPDNVPPKQTPVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DR06</accession>
    <name>A0A3B4DR06_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Fused homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">STK36</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017557261.1">
        <property type="nucleotide sequence ID" value="XM_017701772.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000026812"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004176.2">
        <property type="protein sequence ID" value="ENSPNAP00000026812.1"/>
        <property type="gene ID" value="ENSPNAG00000012151.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429765"/>
    <dbReference type="CTD" id="27148"/>
    <dbReference type="GeneTree" id="ENSGT00940000158375"/>
    <dbReference type="OMA" id="ADIFRQM"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14002">
        <property type="entry name" value="STKc_STK36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000292">
        <property type="entry name" value="Serine/threonine-protein kinase 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22983">
        <property type="entry name" value="PROTEIN KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22983:SF6">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="4"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="282"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="399"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="850"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="858"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1293" mass="142528" checksum="167133DA12B810A0" modified="2018-12-05" version="1">MEQYHVLEMIGEGSFGRVYKGRRKFSGQVVALKFIPKVGRSEKELRSLKREIEIMRGLRHPNIVLLLDSFETEREVVVVTEYAEGELFQILEDDGSLPEGQVREIACQLVSALYYLHSNRILHRDMKPQNILLGKGGVVKLCDFGFARAMSVSTLVLTSIKGTPLYMSPELVEEKPYDHTADLWSLGCILYELHTGAPPFYTNSIFHLVQLIVKDPVKWPENMSEGCMSFLKGLLTKDPQKRLAWPDLLKHPYVADGVIVLSDEGSSSPLTVIPSPDVQALKQQQAATKSVPRSGEGKLLRRAKEQRDKRKNRQDLTENGKAVCSSQPRSKTAPAGEAPTSGHSLGSTFTVCRNSAHPAANQHQANDIGVARVSSASCKGPISMDYEREFPSVEVGPRQVLRRSGPGHSGLASVRMDSDEQDPDSDEEWQRLAEVTDWANHVSVPVDLPFHQKLKSKLLRSKDQILRETLEEPSSIVPILKLLSNITLTSDPADARRVCEGLELPRFLFGLIVEMHSSPEVMKKPWSERIVGELMAVLLTSWEKNPEWIIKEKRAEDLCQLFVAAFLCPDPNCLAPLAAAILSLFAHRGVCVKVRLDSLTALLGCVLSGPEESYHPLPAGWGMCDGVLALILYCLTESESCALRDFLNSELWCHLWTKVGTTLQNMTSKTLFLSTNGLYVFLSLALFAFSNEPYDCVALFFDKDKNLMRSLSLLLTTDSSATELKRGLLWGSSDMSSLSVMSCHLLCFPFALELPQEKMMEILHSYQSQNVVTGLVQVIQTQPPALLELPLSLLCRLCLSSPQGAVPSFTKAARASGFLPQSAETEQILNHLNQSGSTAEQPRNNGIQHKNVFNLSRGDGDCHPKRGIDMIRGSTDKSRGMRDPPKVQSQLSKSKCAFGKLRERAEQPKTKQDHKILSMEHLQNSMDQHSHSVDEFVDSLELHEGFSHLKHFPTQHKSSLSWLIDSLEQPRRSLDHGRGDGDCLRVSEVKGQSPEPAVRTASSLLSMLLQSESLCGCAMQLLTLLSQTACYPAALPVDPALLRIALSHCDDGVRAASCSLLAQLEQHWDHSTNGMKASPNWTIEPALFQDLLCRLTDPAPSVRRIACKAAERWLSLMGQTVFKSLPKRDQHLQEGPGTKKKTVCVPAGKGRGKRSVEVEGIRQGGTGNSGSSLKPVGPYVEGEEWVKVAMGAACPTVSLLLDSDAVIRRRACVILGNMAVVGGGDRALISADAPRQVLRVARGDSHHAVRRQALATLCAFGHLDTLQQALKSIDAGLKLHHVSQGSSLPSNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DSS7</accession>
    <name>A0A3B4DSS7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550256.1">
        <property type="nucleotide sequence ID" value="XM_017694767.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017550257.1">
        <property type="nucleotide sequence ID" value="XM_017694768.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017550258.1">
        <property type="nucleotide sequence ID" value="XM_017694769.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037402621.1">
        <property type="nucleotide sequence ID" value="XM_037546724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DSS7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026161"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005931.2">
        <property type="protein sequence ID" value="ENSPNAP00000026161.1"/>
        <property type="gene ID" value="ENSPNAG00000001723.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425808"/>
    <dbReference type="CTD" id="393726"/>
    <dbReference type="GeneTree" id="ENSGT00530000063405"/>
    <dbReference type="OMA" id="MRFPNKQ"/>
    <dbReference type="OrthoDB" id="5855206at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17045">
        <property type="entry name" value="Ubl_TBCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000837">
        <property type="entry name" value="Tubulin folding cofactor E-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000115">
        <property type="entry name" value="tubulin-specific chaperone cofactor E-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000145">
        <property type="entry name" value="Tubulin-specific chaperone cofactor E-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047991">
        <property type="entry name" value="TBCEL_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="363"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="445" mass="50359" checksum="6293D33BD85B2D9E" modified="2018-12-05" version="1">MSSTCVVSDVSPVLDRAMEAPEGEGRTFMQVISEKYSPENFPYRRGPGMGVVVPSGHQGSPMKDRLNLPSVLVLNGCGITSAGDEGEIAAFCAHVVELDLSHNKLMDWHEISKIVANIPNLDFLNLSSNPLSEAVLEPDYAEAFSRVRRFVLNNTQVSWDVVHTFTREMPELEELFLCLNEYNTVTASATPCTSLRLLHITDNSLHDWSEVRKFGSMFPSLDTLVLANNNLSSILESGDNLCRLFPNLRSINLHNSGLNRWEDIEKLNFLPKLEEVRLQGIPLLQTYTNTERRSLMIAQLASVTCLNGSVVSKGEREDAERFFIRYHLDHPEEELPHRYHSLVTKYGKLAPLAEIDLRPRCHAKVEVHCEDRVEQVSIRLDQTVAELKKQLRTVVQLPTSNMRLYYIDKDMCSAFGPEEMKYSTRALHSYSIRDGDEILVVPKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTT5</accession>
    <name>A0A3B4DTT5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580895.1">
        <property type="nucleotide sequence ID" value="XM_017725406.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTT5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026501"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004942.2">
        <property type="protein sequence ID" value="ENSPNAP00000026501.1"/>
        <property type="gene ID" value="ENSPNAG00000005980.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444303"/>
    <dbReference type="GeneTree" id="ENSGT00940000156538"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016312">
        <property type="term" value="F:inositol bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09094">
        <property type="entry name" value="INPP5c_INPP5J-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2840:FF:000005">
        <property type="entry name" value="inositol polyphosphate 5-phosphatase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000066">
        <property type="entry name" value="Inositol polyphosphate-5-phosphatase Kb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041611">
        <property type="entry name" value="SKICH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF117">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 5-PHOSPHATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17751">
        <property type="entry name" value="SKICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="78"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="548" mass="62961" checksum="5D6554B9B5823967" modified="2018-12-05" version="1">MEEITTHFSTKMRPRSDSVASTGTQSSGSRMLLRQRLTMLMTCVDDLDPKDKIHVQMAKTLDGAFQICGQRAEQHKKDQFRLYIVTWNVATAEPPDDVASLLQLNSSKKADLYVIGLQEVRAAPLKFMTDLAFDDSWSHLFMNTLAPLGYIKVSSIRMQGLLLLFFSKIEHVPFIRDIQVTYTRTGIYGYWGNKGGVTIRLSFYGHMLCFLNCHLAAHMKYASERVDEFEYILDSQTFQGKKASSILDHKVVFWFGDLNFRIEDHGMHFIRNCITSKNFNLMWSKDQLTVMKKNVPKLQQFEEGPLDFQPTYKFDLNSDNYDSRVQKAWFGLTGKKRKPAWCDRILWRVKPRPPPPEDIFEEGDDDAAKEKKKNLLEDLMEKYPLKVVQDSYTSSMEYGVSDHKPVIGMFRLELRKMYDIPLVRLHAEGDWSADLDAMVTYSPLQPFPSSAWDWIGLYKVGFRSISDYITYTWVKDDQVAFNDEVVQVYVSKEEIPVVGGDCVLCYYSSNLQCIVGISEPFKVHESRAAIEEGLLPENINGLDQAIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXD0</accession>
    <name>A0A3B4DXD0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565770.1">
        <property type="nucleotide sequence ID" value="XM_017710281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXD0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027649"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002024.2">
        <property type="protein sequence ID" value="ENSPNAP00000027649.1"/>
        <property type="gene ID" value="ENSPNAG00000002609.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434858"/>
    <dbReference type="CTD" id="557584"/>
    <dbReference type="GeneTree" id="ENSGT00940000153763"/>
    <dbReference type="OMA" id="WIHDFAK"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1280.20:FF:000001">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1280.20:FF:000002">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF7">
        <property type="entry name" value="H(+)_CL(-) EXCHANGE TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0129">CBS domain</keyword>
    <keyword evidence="13 15" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="383"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="529"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="575"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="17">
        <location>
            <begin position="661"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="17">
        <location>
            <begin position="755"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="822" mass="90745" checksum="FF98B8CB1E1A5D9C" modified="2018-12-05" version="1">MKAPGMAQPERVENGSFCKQGSDSLDDELVDLVSAEQDLPEDVPYLDRDHTAGYSNGFRGQVASAGLLDLLDEGVPGVGTYEDFNTIDWVREKSKDRDRHREINRQKKQSDLALLLSLIDAFSGWLLMLLIGLLSGALAGGIDIAAHWLTDLKEGFCMKGFWFTHEHCCWKSNETTFQRRNKCPQWKSWAELMVGVPEGASAYVVNYLMYVSWALIFSYLAVILVRAFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTITLVLAVSSGLSLGKEGPLVHVACCCGNILCHLFTKYRKNEAKRREVLSAASAVGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWHFLELFPFVLLGIFGGLWGALFIRANIAWCRRRKTTRLGHYPVLEVVVVALATAVLAFPNEYTRMSSSELISELFNDCSLLDSSKLCDYISPGKGGERAANFSMTTNGLADRAAGPGVYTALWQLVLALVFKMFITVVTFGMKVPSGLFIPSMAVGAIAGRLLGIGMEQLAYYHHEWPMFKGWCSLGAKCITPGLYAMLGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAATMTSKWVADALGREGIYEAHIRLNGYPFLEPKEEFEQKTLAMDVMRPRRNDPPLSVLTQDGMTVEEVEGMISDTSYSGFPVVVSHQSQRLVGFVLRRDLVISIDNARCCQDGIVGASRVLFTEHTPSHPYQGGPPPLSLRPILDLSPLAITDHTPMEITVDIFRKLGLRQCLVTQNGCLLGIITKKDILKHMAQMANRDPDSILFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DYB9</accession>
    <name>A0A3B4DYB9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">VPS35</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DYB9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028453"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014634.2">
        <property type="protein sequence ID" value="ENSPNAP00000028453.1"/>
        <property type="gene ID" value="ENSPNAG00000014204.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007315"/>
    <dbReference type="OMA" id="YIRSREY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031748">
        <property type="term" value="F:D1 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099639">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903829">
        <property type="term" value="P:positive regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099175">
        <property type="term" value="P:regulation of postsynapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="825" mass="94953" checksum="98AF9987CC569B3C" modified="2018-12-05" version="1">MPTTQQSPQDEQEKLLDEAVQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVRSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDDGEQAEGEMTGDINDSIDFVLLNFAEMNKLWVRMQHQGHSRDREKREKERQELRILVGTNLVRLSQLEGVNVEKYKQIVLPGVLEQVVNCRDSLAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPERVDYVDKVLESTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLQLKHFPPLFEYFDYESRKSMSCYVLSNTLDYNTTIVAQEQVDAILNLVSTLIQDQPDQPAEDPDPEDFAEEQSLVGRFIHLLHSEDPDQQYLILNTARKHFGAGGNQRIRYTLPPLVFAAYQLAFRYKENSSSDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMRCFSEENHEPLRTQCALAASKLLKKPDQCRAVSICAHLFWSGRNTDKNGEEIRDGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYVCFYERENDAVTVQVLNQLIQKIREDLPNLEASEETEQINKHFHNTLEHLRLQRESPESEGPAYEGLVLCVFPLQVHTWPHRITSEKEKGQSRQSRCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZM9</accession>
    <name>A0A3B4DZM9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Alpha-amylase</fullName>
            <ecNumber evidence="5 14">3.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="5 14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZM9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028913"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015520.2">
        <property type="protein sequence ID" value="ENSPNAP00000028913.2"/>
        <property type="gene ID" value="ENSPNAG00000003252.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154802"/>
    <dbReference type="OMA" id="GTHGVQS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11317">
        <property type="entry name" value="AmyAc_bac_euk_AmyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000020">
        <property type="entry name" value="Pancreatic alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000056">
        <property type="entry name" value="Pancreatic alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031319">
        <property type="entry name" value="A-amylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43447">
        <property type="entry name" value="ALPHA-AMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00632">
        <property type="entry name" value="Aamy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0868">Chloride</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044005761" description="Alpha-amylase" evidence="16">
        <location>
            <begin position="22"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="18">
        <location>
            <begin position="32"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-amylase C-terminal" evidence="17">
        <location>
            <begin position="429"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="517" mass="58119" checksum="B094FE9C55370F20" modified="2024-11-27" version="2">PLKGMEMKLLVLAGLFGLSLGQHDPHMKHGRTSIVHLFEWRWADIATECERYLAPKGFGGVQISPPSESIVVTNPWHPWWQRYQPISYNLCSRSGTEEELRDMITRCNNVGVNIYVDAIINHMCGTGAGEGHHSSCGTYFDANKKNFPSVPFSSLDFNDGKCKTANGGIENYHNIFQVRDCQLVGLLDLAMEKDSVRGKVAEYMNKLIDMGVAGFRMDACKHMWPGDLSAVYDRLHNLSTKWFSSGLKPFIYQEVIDLGGEPIKASEYFGLGRVTEFKYSAKLGTVVRKWENEKLCYLKSWGEGWAFMPSDKAVVFVDNHDNQRGHGAGGASILTFWDARLYKMAMGIMLAHPYGVTRVMSSYRWDRNFENGKDKNDWMGPPSHGNGSTKAVPINPDTTCGDGWVCEHRWRQIRNMVNFRNVVDGQPISNWWDNGHQIAFGRGSRGFIVINNDDWNLRETLSTGLPGGIYCDIISGDKLGGGCTGKQIRVDKDGRAHFSISNWEDDPFIAIHVEAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1N4</accession>
    <name>A0A3B4E1N4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch domain containing 7A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KLHDC7A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1N4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029241"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016165.2">
        <property type="protein sequence ID" value="ENSPNAP00000029241.2"/>
        <property type="gene ID" value="ENSPNAG00000015196.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162724"/>
    <dbReference type="OMA" id="PLTSIWK"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052310">
        <property type="entry name" value="Kelch/BTB_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45972">
        <property type="entry name" value="BTB_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45972:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="30"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="326"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029241.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="84444" checksum="11FD4FE6A741C23E" modified="2024-11-27" version="2">MQLLGKLTLSVAAVLLISWAYRFYSSRAQGQTQTDSRIPLPRGTRTGPETCSNCKTVLRPQNKTAKEETNGESQSRQSSVDSEQNIFKSEKSNALLKREPGFDTAEQSTTAGQSNAEDFYQNKEKTKGETKDCKSGLTLLLPDEADKTVGSPTGRRSPCLLRKLDGGVEVGRELRQDLGHLGSFSSFKSKAEIKVENADLVREGPGDQTTGVRGRIYDYYVETSSHSVSDLSPPHSLSPTFGPYETGTLFDAQPSDLSSIVDKPRTPSPLPSSLIIRDLEVSHESDVEPISPIKSESVKRLRHGLIRKDSYLAAAVDSEVQIPIPPPLSSTPCGHSAALSSEVSQSSSGTNKETQDLRRDATEEPQVETVAGAKFLNFPQENMGSTELESLTGKLDLGNCLETLMLAKKHRHSALQEAALRVMSDNYFQVLRDPALFGRLKAGERDQVQRQRMRGRRCLMVADMESPEWSRLSSQSTVSEANKVSSGLYYYDDYKDIWHLQCQIPQEVLSRGCAMCTMDNYLFVAVGFQGTDRVAVPSRKVFCYNPMTSIWSEICPMNEARPHCKLVTLQDHVYAIGGECLSTVERYDPRTNRWSFVAPLPNDTFAVAHRATACNGELFVSGGTLRYTLLRYNPKTDIWRQSLIVGSKERTTEMVAVRNFLYRFDVSPSMGISVYRYHAVARLWYECCSKRMPHCPAFQCVAMDDIIYCVSRQFTMRFLADEISPGFVAEDLKVLSAAKGILFPFVLALPDKSTIQTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E202</accession>
    <name>A0A3B4E202_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NEBL</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E202"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035500.2">
        <property type="protein sequence ID" value="ENSPNAP00000029356.2"/>
        <property type="gene ID" value="ENSPNAG00000015409.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154775"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000087">
        <property type="entry name" value="LIM zinc-binding domain-containing Nebulette"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051759">
        <property type="entry name" value="LIM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000900">
        <property type="entry name" value="Nebulin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218">
        <property type="entry name" value="LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218:SF3">
        <property type="entry name" value="NEBULETTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00880">
        <property type="entry name" value="Nebulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00227">
        <property type="entry name" value="NEBU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51216">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029356.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="285" mass="32849" checksum="A9089ADDF5EF1B91" modified="2024-11-27" version="2">MNPHCARCGKIVYATEKVNCLDKYWHKACFHCEVCKMTLNMNNYKGYEKKPYCNSHYPKQTFTIVADTPENLRLRQQSELQSQVKYRKNFEESKGRGLRYLVDTPEVQRLKEAQDQISNVKYHKDFEVPGLHGVTQGMRGAYLVRAQGRPNHQTMGSGAAQQDVHQYIMEMDRRPGVIVAPVLPGAYYPSGHSQGHSYMHQTSMHSLRSMQLRSHPATMVSSCPTDHFLFVFIYFNLHVFSPIIHLQLYSVIKSNQIYLCSAFYNGCCHKAASKNSRKDKVLTRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E283</accession>
    <name>A0A3B4E283_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FOS like 1, AP-1 transcription factor subunit b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E283"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029855"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017353.2">
        <property type="protein sequence ID" value="ENSPNAP00000029855.2"/>
        <property type="gene ID" value="ENSPNAG00000016223.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160034"/>
    <dbReference type="OMA" id="WMEHENS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
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        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
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        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF25">
        <property type="entry name" value="FOS-RELATED ANTIGEN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="107"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="128"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029855.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="181" mass="20436" checksum="C698C61923025A67" modified="2024-11-27" version="2">MYQSKAGAAFSYGPYTEMSARLDLNVHKTLTDLATSSPNLDTVTSSPNLQWLLESSVVSEAEAVWETLASFLPSTLPNCPCVSQCSSPNLLHSGTARTPESKAVEECERRRARRERNRIAAARCRDRRRMLTDTLQNETERLERVKSQLEEEIAGLERERERLELVLEAHRPACKMNGSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E6R7</accession>
    <name>A0A3B4E6R7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATPase family AAA domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ATAD2B</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000031450"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020445.2">
        <property type="protein sequence ID" value="ENSPNAP00000031450.2"/>
        <property type="gene ID" value="ENSPNAG00000018946.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074694"/>
    <dbReference type="OMA" id="DKQCTTN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05528">
        <property type="entry name" value="Bromo_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19517">
        <property type="entry name" value="RecA-like_Yta7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000021">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000699">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF5">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 17" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Bromo" evidence="19">
        <location>
            <begin position="953"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="199"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1059"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1284"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="218"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1132"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1146"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1242"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1290"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1307"/>
            <end position="1318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031450.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1394" mass="157244" checksum="594DF7D4C864ABB0" modified="2024-11-27" version="2">MVNTRKSSQEKPGSNFISCRTRSSPRNNPKLEEQIHVRKDTSSDVRSGSPRLSPPSKRARRQTPHCSSDTSPSPQSKGQRVTWNITKHCTRLSTGALQNGHRRMTLRGDDSTDGDDGHVNGHVELKRSCRSRKSRFEHLNQSLLFDQLVNSTAEAVLQEMDNISSIRQNREVERLRMWTDTELENMDMYSRVKRRKAMRRNTFGMQTHHKVISKSEAPEEEEGTEESHEEGDEDDDGEDDEDDEGDEAEEAGEENDRPYNLRQRKTVQRYEAPPIEPVNRKQSKNTLFDTHRSPARRSHIRIKKHAIHSSDTTSSSDEERFERRKSKSMTRARNRCLPMNLRAEDLASGVLRDRVKVGASLADVDPMNLDTSVKFDSVGGLADHIKSLKEMVVFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDRKVSFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDSRGEIVVIGATNRLDSIDPALRRPGRFDREFLFSLPDKKARKHILKIHTRDWSPRLTEPFVDELAEKCVGYCGADIKALCTEAALAALRRRYPQIYHSSQRYKLDVGSIELGPQDFSRALRSIVPAAQRALAPPGQALSCTLQPLLERSLAHALDCLLRVFPHAELPHREHTYDTDNQLLEEDSYSEGEDGAGAQSIYDAMPGSPKKMDSSTTHKPFLHFTTSAYQQPTSYRPRLLLTGPQGSGQSTHLAPALLHHLDKFTVHRLDLPTLYSVSAKTPEESCAQVFREARRSVPSVIYMPHISDWWEAISETVKSTFLTLLLDVPSFTPILILATAESIYQQLPEEVKCIFSRSYGEVVCLRTPGEEDRTKFFSDLILVQAARPPPRQRKKVCDYEVLPLADDPGPRQLSPEEQRRLEEQEENTLRELRLFLRDVTKRLATDKRFQIFSKPVDIEEVSDYLEVIREPMDLSTIMMKIDTHKYTTAKDFLMDVDLICSNALEYNPDKDPGDKIIRHRACSLKDTAHAMIASELDPEFDRMCEEIKESRRKRGIQTPLQQAPAPAAAAGKRPTGEEATGNTSQVDGVEKHCSNSVKRKNRRRPIWERGIIRKKKSSKKEEAEEEEEPDEEEGAEDSAGAGQGEASLIQDESSSDTMEPQPDKQLPQPNGYALSTEEESSNEPIVTQDPGDFDKVKTATSNEAPHGEEKEASVVSAPQDCVNGDESLDSIDSEAHEKELEKGGKEVISPLKGNAQDGIGDAVQECKTNECMSAAEGAASDGDHDREGTSKGKKCRKQQKEVVGERPEEPPQPPPPPALVVDHQRLKALLDQIVMKSEGYSVDQLERLYSLLSQCIYQHRRDYDKTHLIEVNASCDFRCDVQKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E896</accession>
    <name>A0A3B4E896_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">JunB proto-oncogene, AP-1 transcription factor subunit a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Jun subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E896"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032822"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023131.2">
        <property type="protein sequence ID" value="ENSPNAP00000032822.2"/>
        <property type="gene ID" value="ENSPNAG00000021060.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161195"/>
    <dbReference type="OMA" id="HFQHAAR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14696">
        <property type="entry name" value="bZIP_Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000012">
        <property type="entry name" value="Putative transcription factor AP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005643">
        <property type="entry name" value="JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF58">
        <property type="entry name" value="JUN B PROTO-ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03957">
        <property type="entry name" value="Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="208"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="206"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032822.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="287" mass="30618" checksum="3C888DA52C56D230" modified="2024-11-27" version="2">APSDLSRFRAVVCDFYAAGAGGAGDPASLKLASPELERLIGGGGGGGVITSPSTGTCTGAGAAPVQQHVYARTIAEDQECFADGFVKALDELHKMNHVPPPNVALGGAGGGSSCAGASVFPDAPVYATLNSCHPNTTLPPAASYPTATISYLPQYHHHHQHQHHHHQPHHFQHAARLAGLKEEPQTVPDLQGDSSPPTSPVDMEDQERMKAERKRLRNRLAATKCRRRKLERISRLEDKVKVLKSDNAGLSSAASLLREQVAQLKQKVMTHVSSGCQLMLAPKIKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EA80</accession>
    <name>A0A3B4EA80_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EA80"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032608"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037432.2">
        <property type="protein sequence ID" value="ENSPNAP00000032608.2"/>
        <property type="gene ID" value="ENSPNAG00000020662.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160115"/>
    <dbReference type="OMA" id="AMDYSHL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000972">
        <property type="entry name" value="TF_octamer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF46">
        <property type="entry name" value="POU DOMAIN, CLASS 2, TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00029">
        <property type="entry name" value="OCTAMER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="POU-specific" evidence="14">
        <location>
            <begin position="167"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="267"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="269"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="326"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032608.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="438" mass="47395" checksum="56669B23F10B62A9" modified="2024-11-27" version="2">MTKTAPVAAMDYSHLWLPDIRMSKPVEVENPAADSPMENTGGTAKVEECGEMSPASVQTTPTQTTLPHTQLMLTGGQLAGDIQQLLQLQQLVLVPGHPLSSPAQFLLPQAQQGQQGLLSTPNLIPLPQQNQGALLAAPPTLGLQAQVREKVMESSASTVTPVTSHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLTDAETMSMDSTLPSPSSLSSPSLGFDGLPARRRKKRTSIETSVRVALERSFCSNQKPTSEEILLIADQLNMEKEVIRVWFCNRRQKEKRINPSNATPPLPSQPQPASHKPPCYSPHMMPSQGPSPAVNSLSAAATTMSSVCPLTPTGPSSSSTPSPVAPSPRSDASPVPASHSTINLNTGSWRNKNGDVSNYITDFVANLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFQ2</accession>
    <name>A0A3B4EFQ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Regucalcin</fullName>
            <ecNumber evidence="7">3.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Gluconolactonase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RGN</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucono-1,5-lactone + H2O = D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="EC" id="3.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.17" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFQ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034041"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038307.2">
        <property type="protein sequence ID" value="ENSPNAP00000034041.1"/>
        <property type="gene ID" value="ENSPNAG00000023080.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014995"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000027">
        <property type="entry name" value="Regucalcin homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008367">
        <property type="entry name" value="Regucalcin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01791">
        <property type="entry name" value="REGUCALCIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="16">
        <location>
            <begin position="16"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="325" mass="36189" checksum="3BE8DD307E9BA963" modified="2018-12-05" version="1">MSSVKVECVVKEPNEIGEGPVWEEKDSTLLYVDITGQKISRWSSLTNQIESMSTAHSNWPETLPAHQEFSRLYQSPTLALETFVGCVVPRRSGGYVIGEGTHFAAVDWQKRSITTIAHVDTDKSKTRFNDGKVDPAGRFFAGTMGLEVRPAEVEKKQGSLYSLQADHSVIKHFDQVDLSNGLDWSLDHRYFYYIDSLKFMLEVFDYDMQTGAISNRRTVYQLEKDEGIPDGMCIDAEGKLWMACYSGGRVLRIDPQTGTRLQTVKLPVARTTSCCFGGKDYTDLYVTSAYKGMDEEARAQQPQAGCIFKVSGLGVKGIPPNSFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHJ6</accession>
    <name>A0A3B4EHJ6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHJ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026920.2">
        <property type="protein sequence ID" value="ENSPNAP00000034774.2"/>
        <property type="gene ID" value="ENSPNAG00000024246.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169325"/>
    <dbReference type="OMA" id="NPKSNMD"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38706:SF3">
        <property type="entry name" value="SI:CH211-198C19.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38706">
        <property type="entry name" value="SI:CH211-198C19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034774.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="158" mass="18527" checksum="8B1CF45C2E1B2A40" modified="2024-11-27" version="2">MIVEELEKTHFGTPPPRHGLVLLVWYVNNCIDNNMVALCDPTEGAYGFHEFKNRGPSTLLPKLKDKMYGYFTLGNLHYKHATDLPYEVRKYYNPHDLKSNMDRVIVKYNKNKNTIEEVYISEHYKKSKTYLLGPKLIEELRQQAAFRIVPEAFSEMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EIX3</accession>
    <name>A0A3B4EIX3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">C14orf132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EIX3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029379.2">
        <property type="protein sequence ID" value="ENSPNAP00000035830.2"/>
        <property type="gene ID" value="ENSPNAG00000026111.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00700000106116"/>
    <dbReference type="OMA" id="DECNTDF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043725040" evidence="2">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035830.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="90" mass="9976" checksum="AB194EE1EC9E2052" modified="2024-11-27" version="2">MFLTEVLLLLLFSQIPVMTGAFMDSSPNDDYSTDHSLFNSSASVHTAAMVAHHQQEEQQPMSRDAIWLWIAITATIGNIVVVGVVYAFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJM1</accession>
    <name>A0A3B4EJM1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Solute carrier family 39 member 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLC39A5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561490.1">
        <property type="nucleotide sequence ID" value="XM_017706001.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017561514.1">
        <property type="nucleotide sequence ID" value="XM_017706025.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJM1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036652"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031557.2">
        <property type="protein sequence ID" value="ENSPNAP00000036652.1"/>
        <property type="gene ID" value="ENSPNAG00000027714.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432268"/>
    <dbReference type="CTD" id="283375"/>
    <dbReference type="GeneTree" id="ENSGT00940000161155"/>
    <dbReference type="OMA" id="HPETHSA"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF17">
        <property type="entry name" value="ZINC TRANSPORTER ZIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017200489" description="Solute carrier family 39 member 5" evidence="8">
        <location>
            <begin position="29"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="363"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="59894" checksum="08C70753EF235817" modified="2018-12-05" version="1">MMEEPNHRTYLSFKIVLHLCLSFVVVLSKHSSVSRSYDQDASTQEHLDEAFEEQGYYLQRLFLEYGSNGTLTYEGLQKLLGSLGLGEVSVLNIRHGVVKNSSPNPSHSHPHSYVDHHPHQASSTAPLNKLSQQETFRPKETMSSAETTVVSRAADHSHPTASPGTEERLQDGLSLLSSHITKKHIHDNCLNVTQLLWNFGLGQASHITPAHFTFLCPALLYQIDSGVCLRHTESDLEEVKNSVAFLKALGWASLALFVISLPSLLALGLAPLIPPSNLHTFLCPMAAMAVGTLCGDALLHLLPHASSRPHSDNQDAVLKGLTVLGGVYLLFVFESVVGLRQHYKKSRKRKQDSKVVQELEALEGSSAPDHTVSSESGHGHSHAPPAHESSGLSSVAWMVVMGDGIHNLTDGLAIGVAFSQSLTGGLSTTIAVFCHELPHELGDLAVLLAAGWPVRRLAVFSVLSALLGYVGVLGGTVLGHQWAQHSPWILTITAGVFLYVALADVMPEMLHGERGSMGPLRRFLLQCAGLLVGGIIMLCIALFEEDIALSIGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKD4</accession>
    <name>A0A3B4EKD4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037398620.1">
        <property type="nucleotide sequence ID" value="XM_037542723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EKD4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029009.2">
        <property type="protein sequence ID" value="ENSPNAP00000035671.2"/>
        <property type="gene ID" value="ENSPNAG00000010606.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415660"/>
    <dbReference type="GeneTree" id="ENSGT01150000287002"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000005">
        <property type="entry name" value="MHC class I antigen ZKA transcript variant 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF193">
        <property type="entry name" value="LOC571647 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043769685" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="211"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035671.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="410" mass="45712" checksum="44FCCA600E5CBD6B" modified="2024-11-27" version="2">MGVSAFICAALLCGEVVFTLGDKHSLYYIYTALSKDVALPGIYEFTALGLLDDRAIDYYNSQEQVKVPKQDWMREKLQPDYWDKGTQSRRSKEQWFKVNVEILMQRMGHNQTNLHVLQWRHGCEIEEANGKPKFLRGIDEYSYDGDEFLSFDDDNMRWIAPVAQALPTKRKWDDVPILNQYTKGYLEKECVDWLTKFMQYGKEALRKHSPPDVHVFAKKCTTDSTKLTLTCLATGFYPKDMVVSLRKYRTALPEHLVKSSGVRPNEDGTHQLRKSVDIQEDEKAKYDCFVNHITLKTPVTKQWVPSSGMVGAVIGGVIVLLLLTSGGIIFALIKKGIIAVPCIGNPVTMATAATALQAPPNGVPEKLMQAGNGAVMTNGTANGHPEEVPLCSNGNEHMHEKGMNGAPASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKF8</accession>
    <name>A0A3B4EKF8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein mago nashi homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD).</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the EJC core complex that contains casc3, eif4a3, magoh and rbm8a.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mago nashi family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576805.1">
        <property type="nucleotide sequence ID" value="XM_017721316.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EKF8"/>
    <dbReference type="SMR" id="A0A3B4EKF8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016605"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039961.2">
        <property type="protein sequence ID" value="ENSPNAP00000036375.1"/>
        <property type="gene ID" value="ENSPNAG00000027135.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441668"/>
    <dbReference type="CTD" id="4116"/>
    <dbReference type="GeneTree" id="ENSGT00390000003156"/>
    <dbReference type="OMA" id="NQTDEFY"/>
    <dbReference type="OrthoDB" id="6495301at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11295">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1560.10:FF:000001">
        <property type="entry name" value="Protein mago nashi homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1560.10">
        <property type="entry name" value="Mago nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004023">
        <property type="entry name" value="Mago_nashi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036605">
        <property type="entry name" value="Mago_nashi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638:SF0">
        <property type="entry name" value="MAGO HOMOLOG, EXON JUNCTION COMPLEX SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638">
        <property type="entry name" value="PROTEIN MAGO NASHI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02792">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89817">
        <property type="entry name" value="Mago nashi protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="17223" checksum="0AF29A474A15740E" modified="2018-12-05" version="1">MSTSDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLRVFYYLVQDLKCLVFSLIGLHFKIKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELB8</accession>
    <name>A0A3B4ELB8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017548494.1">
        <property type="nucleotide sequence ID" value="XM_017693005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELB8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036059"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039688.2">
        <property type="protein sequence ID" value="ENSPNAP00000036059.1"/>
        <property type="gene ID" value="ENSPNAG00000026558.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424776"/>
    <dbReference type="GeneTree" id="ENSGT00650000093445"/>
    <dbReference type="OMA" id="EYPPRPM"/>
    <dbReference type="OrthoDB" id="1867783at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000679">
        <property type="entry name" value="Homeobox protein aristaless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042988">
        <property type="entry name" value="NOBOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47060">
        <property type="entry name" value="HOMEOBOX PROTEIN NOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47060:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN NOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="237"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="239"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="655" mass="71478" checksum="EF6F427821024959" modified="2018-12-05" version="1">MTEDSLFASDYDGPCPLCEEEGERPSLGLRVSERERKVDKEREEAELKREKIKGDKSVGEKGNAKEEVVEQKQELKVGGKTDDEHGEEETDEESIAEKLKVKGDKSDGGKDTYASPPLDGRSAPQLDSGCPVNLVMMCEESVERCPIAQTKQTQATAQMPGPEALCLGNRNNSPSAEPKTLVPPGQLEVTLRQVYTTRRYTRFTSRPTPLLPLPSVCPDSSSQPLPYITSTSLLPPAPKKKTRTSYSSDQLEELERVFRDDHYPDAEKRKEIAMSVGVTPQRVMVWFQNRRAKWRKTSKTTAKKPSASRAPAPALSRAPAPVHRAPVFSAPTIAPIPSQTANALPSYSALFTSCTSPPDPGCVDGGNVCISQGGPVDYKLPLMQSPPPLRRASLPFFAAYNPPTHTLSVLLDTPEHNEPQPLSLHTETGFDYDGIGCSAKLESVISGPGNRAQAYQISTFPQQPSALFPQQPNSLLAQQGNIIPQQPSTLLPQYSHLSYLTPSPYLTPNPSESTTSSCLPQIPGTTSSLMTCTNGGHSYFKCQNGNQILLQPGVQALQTYPWASDVYGHTGHVPHAVLRPQFASQNCEGHYPPLLQPHRYITPQRASCPSLSKPASPDPLMPSVKTELEDAGQGQPARVNEAETVFHCDFSPIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EP70</accession>
    <name>A0A3B4EP70_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sal-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Sal-like protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable transcription factor.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Transcriptional repressor involved in organogenesis. Plays an essential role in ureteric bud invasion during kidney development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">May associate with NuRD histone deacetylase complex (HDAC). Interacts with components of HDAC complex including HDAC1, HDAC2, RBBP4, RBPP7, MTA1 and MTA2. Interacts with CCNQ. Interacts with NSD2 (via PHD-type zinc fingers 1, 2 and 3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566856.1">
        <property type="nucleotide sequence ID" value="XM_017711367.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037386804.1">
        <property type="nucleotide sequence ID" value="XM_037530907.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000036981"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032361.2">
        <property type="protein sequence ID" value="ENSPNAP00000036981.1"/>
        <property type="gene ID" value="ENSPNAG00000028248.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435487"/>
    <dbReference type="CTD" id="560775"/>
    <dbReference type="GeneTree" id="ENSGT00940000155938"/>
    <dbReference type="OMA" id="LPCANAR"/>
    <dbReference type="OrthoDB" id="8749569at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003337">
        <property type="term" value="P:mesenchymal to epithelial transition involved in metanephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021772">
        <property type="term" value="P:olfactory bulb development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000689">
        <property type="entry name" value="Spalt like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000961">
        <property type="entry name" value="Spalt like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000025">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000260">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000079">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000215">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF46">
        <property type="entry name" value="SAL-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="501"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="752"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="780"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="812"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1143"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="1171"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="84"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="307"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="589"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="966"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1286"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="339"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="625"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="977"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1004"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1330" mass="142030" checksum="FABF2B180B3354F1" modified="2018-12-05" version="1">MSRRKQAKPQHLKSEDEASDLGGLSQNVSGEVLDDADSGNESRSGSEETHVCEKCCAEFFKWSDFCEHLKSCTKNPLVLIVNENEGTPDPAREYPAEPSPVPSCPSEPALSEEAAEGRHTPVEADEGTEMTLERAATLEKEDEPMEVELSPEKPMDPEDPEASPDPDVSLPQLEDTAHPSGAGYSMPNTNVTLETLHGTRVAVAQFSQSFRGAVASGVSTMAIPMILDQLMALQQQQIHQLQLIEQIRSQVALMNRQAPSQSALNHHAHHNTNSASQAASSTGLPTISSQLQLHGFITPPVHQLPIRVPPTLNGQGPASLSSALEGSHSHVSQTGGQLSSSVMNSNTSTNSSYPPSSCSVVPSLLPPSSVTSSSNNSSSSAGAGISSSVSLPRNTSSPPTLTHGSLLSSPSNLPLIPHSSSSSVIFPNPLASIAATANALDPLSALMKHRKGKPPNVSVFDTKPSSDDPFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIPMNPYPVPEYLDNVPTSSGIPYGMSLPPEKPVTTWLDSKPVLSTVPTSVGLQLPPTLPTMIGGYGDSPSLTPINRSPQRPSPPSSECASLSPNVLSTEASTTLTSASPQPNIGGDVPPVLKPEGIHLPPNCTTRPGETSLSSTTVTQVVLSATVTTTTTTTTQITDSVTPPSSVTHPSLPMISDQFKAKFPFGGLLDSMQTSETSKLQQLVENIDKKMTDPNQCVICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHFGVHRSKPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPLPEGFQEMDTDFSFDEKSLDNMSNYDDELIDEMEQAMEDEPDMKDGDMEPSKLTLSYGEMSPSSSPPTSVISSIAALENQMKMIDSTANMTRAFNLKPSENGSGVGGESGDMLAIDSSSAVGDLENHSVGSPALSDSSGSMQALSPAHSQSESHRSRSPGLANNSSSNAAEDGQECSTATATVKSEKSETPSPGSVPAEHIGALDLTAAQPTRPYVKEENQFSMLFLGRDRGLNAPSLMSSAPSMVKLEMNGHSKPLSMSESSHLHMSLQVPAATPQTTMSPNLTPMLAPPPPRRTPKQHNCQSCGKNFSSASALQIHERTHTGEKPFACSICGRAFTTKGNLKVHMGTHMWNNAPARRGRRLSVENPMALLGGDAMKFSEMFQKDLAARAMNVDPGFWNQYAAAITNGLAMKNNEISVIQNGGIPPLPVSLGGSGIPSLGSMSGGMERARTGSSPPMTGLEKTSLEVGTGRPFSRFLEDSKEIGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IRE1</accession>
    <name>A0AAR2IRE1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Formin homology 2 domain containing 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037398756.1">
        <property type="nucleotide sequence ID" value="XM_037542859.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000066283.1">
        <property type="protein sequence ID" value="ENSPNAP00000040216.1"/>
        <property type="gene ID" value="ENSPNAG00000008160.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413230"/>
    <dbReference type="CTD" id="564742"/>
    <dbReference type="GeneTree" id="ENSGT00940000154807"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055003">
        <property type="term" value="P:cardiac myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000056">
        <property type="entry name" value="FH1/FH2 domain-containing protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000004">
        <property type="entry name" value="Formin homology 2 domain-containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056771">
        <property type="entry name" value="FH3_FHOD1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041387">
        <property type="entry name" value="FHOD1_GBD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF3">
        <property type="entry name" value="FH1_FH2 DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24959">
        <property type="entry name" value="FH3_FHOD1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18382">
        <property type="entry name" value="Formin_GBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51231">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="9">
        <location>
            <begin position="18"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="10">
        <location>
            <begin position="991"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="domain" description="DAD" evidence="8">
        <location>
            <begin position="1494"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="411"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="513"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="614"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="810"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="927"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1370"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="558"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="614"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="631"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="678"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="700"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="715"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="738"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="754"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="776"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="819"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="831"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="873"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="944"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1372"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1405"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1436"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1452"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1520"/>
            <end position="1535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1555" mass="174238" checksum="5D249BA577B51FC3" modified="2024-11-27" version="1">MASFVCRVQFLDDTDPFSSTSFPEPARPPRFSFREDLPLSSQLPALHRLLRAPHRLDDCALQLSHNGTYLDLEASLAEQRDEVEGFQQDDECGGGRGKKHSVILRTQLSVRVHACIEKLYNSAGRDLRRALFSLKQIFQDDKDLVHAFVMAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVIDHPETIQWLYALIGSKFRLVVKTALKLLLVFVEYSESNAALLIKAINTVDFKRGRKQWSNAMEILSEKDGVDTELLVYTTSLINKTLAALPDQDSFYDLVDVLEEQGMQEVTRRYIGRKGTDLDLLEQLNLYEATLRHEDGDDEGQAPPTGGRDRRRSSVGGVEGRGLERRRSRRHSLGRMGSSTPLSPTSPHRTNFHPFNGEVSEREAVCESKPLMVIEEECEDEEETSVTGSDDDDGDDDEDGSSKPVSQSVISTVSKEVEERRKSAEVSSGGAAEQPSLLASLLARRKSCVSVPSCSEVSPSRPLQRLPYLPHSPFHLFSYDMQEEEEPSQRSGGRSFSAAPSTPTKQQSDRPPLGGLLSSSYRQHQESLAAERERRRVEREERLQRIEREERNRHSREYVVNMEEARLAREERFKTVERLAAEEYERDHVRLAPRSLTLSPTDICQSSDGDTPTSVTSQDDQDTSSHLPCEPEPDSQASPEPEQKEEELTEQEDEAEEEEEVEESSVDKEEGGPDERAGLVEEEEEELEQEAPDAEAEDSGILSDKERQNEEVNEKDNCSASSISSSSSTLEREERAHGNVAETSETGPSGQEGSASEQCSEILNSKLFMLDMLYSQGSKQRSQEEEEEAEEEAETEKEKEGERTVQEAGGEEEEKKKLEGGMVRAFAERFGDLVKGLSSPPMMEQESPEKQQQPPQAPPPPKKESDYIWDQLMASPRELRIGDIDFTDLVDEDDQDVLDSGDLIGNSNLPPPPPPPPCPFYLPPPPPPLAPGVRPPPPPPCFSAPTPPSPQLGRPEPPLFQKKKKTIRLFWSEVRPAEWQFLGHKRGHLSLWSKLEPVKLDTPKLEHLFESKSKEVPAAKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKSAILNFDEYALNKEGIEKILTMIPTEEEKQKIQEAQLANPDIPLGSAEQFLLTLSSISELSARLQLWAFKLDYEATEKEVAEPLQDLKEGMEQLEKNKTLRYVLSTLLAIGNFLNGSNAKGFDLSYLEKVPEVKDTVHKQSLLHHVCSAVVENFPESTDLYSEIGAVTRSAKVDFDQLQENLTQMERRCKASWENLKVIAKHEMKPVLKQKMSDFLKECAERIIILKIVHRRIINRFHSFLLYLGHPPYGVREISVHRFSKIMSEFALEYRTTRERILQQKQKRADHRERNKTRGKMIMDVAAPTDDEEENETGQTVGGSSSSSVGGSSTSAPSGGQSEVQGLSHAEDAAEHEHMKAVLKSSLQGGSTESSVLPGLRTRTRVRPSRGRTAWAAANEEGPGVTDDAADEIMERIVRSATQGPRERTQPRERRRSRANRKSLRRTLKNGLTAEEAQALGLEASEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWE6</accession>
    <name>A0AAR2IWE6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017578599.1">
        <property type="nucleotide sequence ID" value="XM_017723110.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWE6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054241.1">
        <property type="protein sequence ID" value="ENSPNAP00000044048.1"/>
        <property type="gene ID" value="ENSPNAG00000036705.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108442862"/>
    <dbReference type="GeneTree" id="ENSGT00940000156265"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF5">
        <property type="entry name" value="KELCH-LIKE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="68"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="646"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="670" mass="74298" checksum="E549BA2C02F334BD" modified="2024-11-27" version="1">MPCEQMNAVKGGTVTWRPQPWHDADGGGGEPLSDSDSEEEDFPDDTTTPLGEYVTQGLKQLLDAQQLCDVTLLVEGKRFMCHRVLLAAVSPYFRAMFTSPLVESRLAEIRLEEVTPSVMETVIHFIYTGEAGLSLDTAEDLFVAANRLQVMPLQDLCSRFLFEHLSVENCLGMYSLARSHHDQLLLRASLRLVAQHFPRVARQRDFLLLDPGTLGSLLESDRLGVESETEVYNAARRWAEHQPAERYIHMPNLLRHLRPGLLAPEESRRLNKELGPRASTEGMGGPLRPREGMFEKKIVCVDLKPREDEALRESDYTVDCFDPRTGKWEKLSALGSLLCPGCTAVGGRLFVAGGILRTGQVSSSVHEYDVVLDQWISRPPMVQPRAMFGLLGSGDSLYALGGCNRSALLDTCEIFDLSKLTWVPGPRLPLPLRCFACAALRGRLYLLGGATLEQNRAVVHSGVLIYHTVTRTWSRVSLDSGATCLAGGVAVRGGVCAVGGYMRDAAKFLDGNYTQLEPLDATGRVLFFREGRGTGAEREILTVTERTGGGGGSDRAPSPVVFPGLPRRIAAGGVARWKRRIYVLGGENGSRFYDSVYCWKPGWRSWVQRREKLPGDTGGVSQFGCTTLKFPKKHILARLRSALEKQGKEKRLNRKTDDRERRTRRANQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYK5</accession>
    <name>A0AAR2IYK5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ALK and LTK ligand 2b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ALKAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017549904.1">
        <property type="nucleotide sequence ID" value="XM_017694415.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017549905.1">
        <property type="nucleotide sequence ID" value="XM_017694416.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017549906.1">
        <property type="nucleotide sequence ID" value="XM_017694417.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYK5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050900.1">
        <property type="protein sequence ID" value="ENSPNAP00000042686.1"/>
        <property type="gene ID" value="ENSPNAG00000032267.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108425626"/>
    <dbReference type="CTD" id="100329254"/>
    <dbReference type="GeneTree" id="ENSGT00940000162505"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030298">
        <property type="term" value="F:receptor signaling protein tyrosine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070378">
        <property type="term" value="P:positive regulation of ERK5 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029364">
        <property type="entry name" value="ALKL1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28676:SF2">
        <property type="entry name" value="ALK AND LTK LIGAND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28676">
        <property type="entry name" value="ALK AND LTK LIGAND 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15129">
        <property type="entry name" value="ALKL1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043322139" description="ALK and LTK ligand 2b" evidence="10">
        <location>
            <begin position="23"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17066" checksum="7A2B1857658EF20C" modified="2024-11-27" version="1">MRALRSPVCVGLLLVILTAGSCRKGSREGSSLLELLMDTGRATRQQYADQSQASGQHPPRRNYSIETGEVNEVNKSYQHDQILEVFPRDLRQKEKFLKHLTGPLYFSPKCSKHFYRLYHNTRDCTIPAYYKRCARLLTRLAGSQRCSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYW7</accession>
    <name>A0AAR2IYW7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fructose-2,6-bisphosphate 2-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.46</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 2,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <dbReference type="EC" id="3.1.3.46"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.46" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017541439.1">
        <property type="nucleotide sequence ID" value="XM_017685950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYW7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069751.1">
        <property type="protein sequence ID" value="ENSPNAP00000043239.1"/>
        <property type="gene ID" value="ENSPNAG00000034490.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413438"/>
    <dbReference type="CTD" id="393160"/>
    <dbReference type="GeneTree" id="ENSGT00940000163946"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF2">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="5">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="295" mass="32267" checksum="EC87D906E33223C6" modified="2024-11-27" version="1">MTSLSCGTLPYEPGRAGRSFSSAAAVASVFIMIAFGLTLVRHGETQYNKDKLLQGQGIDTPLSEVGVRQAEAAGHYLRDIRFTNAFVSNLQRAVQTAEIILRNNVHSSSVEMVLDPLLRERGFGVAEGRPKEDLKNMANAAGQSCRDFTPPGGETLDQVRTRFQKFLKYLFQRILDNHCHAAQHVCPSSIPTLPESLPPVVAGLADDGVADLLFHTLVVSHGAYIRVAVHHLVEDLHCSLPVGLKMNQVYSPCPNTGICRFTFTLDADVSGPQPTAVHCVFINRRDHLAILKDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0G5</accession>
    <name>A0AAR2J0G5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ral guanine nucleotide dissociation stimulator-like 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RGL2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037390017.1">
        <property type="nucleotide sequence ID" value="XM_037534120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0G5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082013.1">
        <property type="protein sequence ID" value="ENSPNAP00000045440.1"/>
        <property type="gene ID" value="ENSPNAG00000005615.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433895"/>
    <dbReference type="GeneTree" id="ENSGT00940000167370"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17211">
        <property type="entry name" value="RA_RGL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF350">
        <property type="entry name" value="RAL GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR-LIKE 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="103"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="261"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="5">
        <location>
            <begin position="624"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="405"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="82248" checksum="673113B0C237AC9B" modified="2024-11-27" version="1">MLHRHMRTAGMDLPGVGSKDVPLIGYRPLQPGTDSSQPADQPGKEAGAKQHAASQVEASPMKTTWYCPLNLNTVCEKEEDGIIFTVVVKQQHGSSNPQTAGQRSQCVKAGTEEKLVLHLLHSFSMEDSSFISIFLSTYRSFTTTQRVLDILMDRLGNPPGESSSPTRQAFHKAVCTVFSTWLSEYPEDFRSLGDPSRLLRLAPLLPPDVAGADLRGRLLRIAEELSEKALIPDQTTDPASSLLQTPPDPARFDATSILGFPSSVIAEQLTKIETELFLKLVPYHCLGSLWSQRDKKGREGVCWSVRATVRQFNRLANAVTASCLWQTELKSQQRSRLLEKWISVAEACRARKNFSSLYAIVSALQSNPIHRLRKTWQETDREAVKRYEELAYIFSEKDNYSQSRELLKEEGTSKSANVDTKTNNRRHTSSAQGTVPYLGIFLTDLTMLDAAVKDRLENGFINFDKRRREFEVLAKIRLLQSSCRNSTFRADESFLHWYHSVPTLTEEESYRLSNQIEAHGEPSPGRALHPTVVITECPDALAVTSSGVDPDGIFDFPSPVNHLLSKLAKHMKSPSVSCLDVDSSPPPTDPTPSTLITPTVVKSHRRSVSCGNAPSNTSPGAGPDMRIVKIRMDLQDGNLYRSILVTSNDKTPTVISSALEKHNQDPREASKYELIQLLPDGKELTIPATGNVFYAMTSASTDFLLRRRGGNTPLGSPSLNETSATFPRIKAKGRRLVRTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J230</accession>
    <name>A0AAR2J230_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J230"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050583.1">
        <property type="protein sequence ID" value="ENSPNAP00000046068.1"/>
        <property type="gene ID" value="ENSPNAG00000031432.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165120"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF17">
        <property type="entry name" value="SUPPRESSOR OF TUMORIGENICITY 14 PROTEIN HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043972372" description="Peptidase S1 domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="31"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="81" mass="8726" checksum="41B5F00588009813" modified="2024-11-27" version="1">MSYCAIVTLSILFAVLSGAGCGTRPRKRAKIVGGRDAQAGSWPWQVSLQMERYGHVCGASLVGNRWLLSAAHCFQDSDAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J6M7</accession>
    <name>A0AAR2J6M7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ELAV-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ELAVL1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the RRM elav family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J6M7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087011.1">
        <property type="protein sequence ID" value="ENSPNAP00000045889.1"/>
        <property type="gene ID" value="ENSPNAG00000010909.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155528"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12769">
        <property type="entry name" value="RRM1_HuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12773">
        <property type="entry name" value="RRM2_HuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12653">
        <property type="entry name" value="RRM3_HuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000006">
        <property type="entry name" value="ELAV-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000005">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000215">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006548">
        <property type="entry name" value="ELAD_HU_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034996">
        <property type="entry name" value="HuR_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01661">
        <property type="entry name" value="ELAV_HUD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043680916" description="ELAV-like protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="51"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="137"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="275"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="357" mass="39662" checksum="20884698D28ED633" modified="2024-11-27" version="1">MDYVLVRCCFLFIICVAQNGILSSKILNLEVYDMSNGYEDHMVDEPKDSKTNLIVNYLPQNMSQDELHSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYLNPSDAERAISTLNGLRLQSKTIKVSYARPSSDSIKDANLYISGLPKTMTQKDVEDMFTRYGRIINSRVLVDKATGLSRGVAFIRFDKRAEAEDAIKDLNGQKPPGATEPITVKFAANPNQVKGTQIISQIYHAQSRRFGGPVHHQAQRFRFSPMGVDHMSSVSGVNVASSSTSGWCIFIYNLSQDTDEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTSKTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J773</accession>
    <name>A0AAR2J773_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">14-3-3 protein epsilon</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J773"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086852.1">
        <property type="protein sequence ID" value="ENSPNAP00000046182.1"/>
        <property type="gene ID" value="ENSPNAG00000009581.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282547"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000002">
        <property type="entry name" value="14-3-3 protein epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="4">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="4">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="267" mass="30276" checksum="1A52379AF25271E1" modified="2024-11-27" version="1">RQIVFHLLLNFTEMVDSMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQTVETELKSICNDILDVLDKHLIPAANSGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTDLQPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGKASCVCCISELNLRCHHLSFSSVAVLNAVYDDCVRYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JBZ2</accession>
    <name>A0AAR2JBZ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JBZ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061674.1">
        <property type="protein sequence ID" value="ENSPNAP00000047852.1"/>
        <property type="gene ID" value="ENSPNAG00000020351.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005508"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000658">
        <property type="entry name" value="PRK00029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12153:SF15">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12153">
        <property type="entry name" value="SELENOPROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="623"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="636"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="675" mass="75388" checksum="F0C85D9A1E526A31" modified="2024-11-27" version="1">MAPIRNYSRGMRTYLSRSGSLALTMMAQAGQTPLQRLRFNNVALRKLPVDASLERGSRTVRGACFSLVEPQPLLRPRFVAVSPRALALLGLSAEDVLTDPHGPEYLSGSALLPGSEPAAHCYCGHQFGVFAGQLGDGAVCYLGEVAAGADRTDPCDRWEIQVKGAGLTPYSRQSDGRKVLRSSIREFLCSEAMFALGIPTTRAGSLVTSDLYVQRDAFYSGNPRPERCSVVLRIAPTFIRFGSFEIFNPADDFTGRQGPSAGLHDIRAQLLDYVIETFYPEIKTEHTDRKARNAAFFREVTVQTARLVALWQSVGFCHGVLNTDNMSILGLTIDYGPFGFMDRYDPEFVSNASDKKRRYTFEAQPYVCRWNLARLAEALGSELQASTAGSILDEFMPLYRNFYLGNMRRKLGLLRREEPEDEELVEDLLKVMHNTGADFTNTFRLLSSVCCPLVGESGQGDVGTVVDLILEQCASLEELKVANHPTMEPRELEMILSMAETSPGLFNMTANRPEVAKQLEKIGRLKELLETNQEDLKVKQRDDWLIWMNRYRKRLTQECDGMSDPAGVDKERVRIMDSTNPAVVLRNYIAQNAIEWAENGDFSEVQRLLKVLENPYSVPPDLKQPIWPGKKGSCETKGSTRQSVMDSGEKKGTADTVCLPYHTKPPEWAREICVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JI95</accession>
    <name>A0AAR2JI95_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JI95"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049370.1">
        <property type="protein sequence ID" value="ENSPNAP00000049924.1"/>
        <property type="gene ID" value="ENSPNAG00000019696.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157462"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14340">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000064">
        <property type="entry name" value="BR serine/threonine-protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048622">
        <property type="entry name" value="BRSK1_2-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF108">
        <property type="entry name" value="BR SERINE_THREONINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21122">
        <property type="entry name" value="KA1_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21115">
        <property type="entry name" value="UBA_BRSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="19"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="345"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="670"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="850"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="529"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="612"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="678"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="898" mass="99319" checksum="63A784C130B1FFC5" modified="2024-11-27" version="1">MSSTGKDNSAQHANYVGPYRLEKTLGKGQTGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRNLLEKVKLGVFHMPHFIPPDCQNLLRGMIEVDAAKRLTLEQIQKHTWYIGGKNEPEPEQPVPRKVAIRTLPSAEDIDPDVLESMHSLGCFRDKNKLMKDLLSDDDNQEKMIYFLLLDRKERYPSHEDQNLPPRNEIDPPRKRVDSPLLTRHNKRRPERKSMEVLSVTEGGSPVPVRRAIDMTQHGQSKSVFSKSLDISDANPKVQSKEERSRSISGASSGLSTSPLSSPRPVRRFFIPPQSSELTQSPNHSPTHSPRANGTGPHPPNAFQPKTGALQPNNKTQTLPAKPKVSEKPLQSTRSNPLPNTTPKPSTAKSEPSTPSTPTHPLPPPIPNSPKVRRPPLTVPPKLSVPLSQAPPLSPIRLHHHHLHPLAGTDHNGKSIPTVQVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPSPTPPPSPSIGGMPWRTRLNSIKNSFLGSPRFHRRKLQVPTQEEMSSLTPESSPELAKKSWFGNFINLEKEEQIFVVIQDKPLSSIKADIVHAFLSIPSLSHSVVSQTSFRAEYKSTAGPTVFQKPVKFQVDITYTESTSATKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHEQPAVQQLSGKHSKGGSPLSSFFDVIKQLFSDEKNGQVPHAAGTPKHGANSKKHESDSNTSKCPSGKDKAKVAPSVGTQEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JTD1</accession>
    <name>A0AAR2JTD1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JTD1"/>
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        <property type="protein sequence ID" value="ENSPNAP00000055265.1"/>
        <property type="gene ID" value="ENSPNAG00000032563.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000245238"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="74" mass="8365" checksum="92990CD0656C2459" modified="2024-11-27" version="1">RKVQHCCTAHQHQNLIPTVKYGGGGMMVWGCFAASRPGRIAVINRKMNSQVYQDILQGNLRPSVFELKQFCMVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KAH5</accession>
    <name>A0AAR2KAH5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD repeat and SOCS box-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">WSB1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KAH5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065429.1">
        <property type="protein sequence ID" value="ENSPNAP00000061280.1"/>
        <property type="gene ID" value="ENSPNAG00000000691.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00890000139406"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03733">
        <property type="entry name" value="SOCS_WSB_SWIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051983">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15622:SF12">
        <property type="entry name" value="WD REPEAT AND SOCS BOX-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15622">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50225">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="191"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
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            <begin position="277"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="339"/>
            <end position="373"/>
        </location>
    </feature>
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        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
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        <location>
            <begin position="406"/>
            <end position="447"/>
        </location>
    </feature>
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        <location>
            <begin position="103"/>
            <end position="122"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040055"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="447" mass="50128" checksum="4BCA0257D3712BBC" modified="2024-11-27" version="1">MLLIHVLKCHFSRVPFLFQKGKRSYIRIIISENFVEYCKAKFIGELLAPVAPFDQKSGRETWTVAFAPDGSYFAWSQGHRIVRLVPWSKCITNFSMRKEERINGAGPRRLSRQGSEGTLVPGEPREHTIDCGDIVWGLAFGSSVPEKQSRCVNIEWHRFKFGQDQLLLATGLNNGRIKIWDAYTGKLLLNLMDHTDIVRDLTFAPDGSLVLVSASRDKTLRVWDLKDDGNMVKVLRGHQNWVYCSAFSPDSSVLCSVGAGKAVFLWNMDKYTMIRKLEGHHNDVVSCEFSPDGALLATASYDTRIIVWDPHTGSVLLELGHLFPPPTPIFAGGANDRWVRSVAFCHDGQHIASITDDRLVRFWSIDEKSPQAIASLTNGLCCAFSTDGSVLAAGSRDGSVHFWSCPRSVASLQHLCRMALRRVLTTQQVYTLSIPQCMQDFLAYRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KBZ4</accession>
    <name>A0AAR2KBZ4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sc:d156</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KBZ4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048068.1">
        <property type="protein sequence ID" value="ENSPNAP00000061800.1"/>
        <property type="gene ID" value="ENSPNAG00000030116.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 24"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
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    </dbReference>
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        <property type="entry name" value="ig"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043815146" evidence="3">
        <location>
            <begin position="31"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="139"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="263" mass="29299" checksum="79B47752440361D9" modified="2024-11-27" version="1">MLKSGMRSRGEMKMLHLLVLTLLTLTGVLSDDGWGVNYSEPICAVRGSDVISYTYFYPKSVKQVKNVLWCSMNSNSGRCYDGSYVYNSDSTAASGDFKYVGDKTSNCTLLISNVQFSHSGEYRFRFITDVKDRRWTGVPGVTLKVAVVQVSMIRLSGNGTLKEGDSVNLTCAVNCTLSSPQFVWFQNKERLTESGSVLHLPALTVRNSGNYFCALRNYEKFMSEIISLDVKGPFPVLGIVWIVLGVILFMLLVAILAVVYKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHL9</accession>
    <name>A0AAR2KHL9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574540.1">
        <property type="nucleotide sequence ID" value="XM_017719051.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHL9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044668.1">
        <property type="protein sequence ID" value="ENSPNAP00000061954.1"/>
        <property type="gene ID" value="ENSPNAG00000005870.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440265"/>
    <dbReference type="CTD" id="101882011"/>
    <dbReference type="GeneTree" id="ENSGT00940000165459"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF127">
        <property type="entry name" value="RAS AND RAB INTERACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23268">
        <property type="entry name" value="RIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="22"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="VPS9" evidence="5">
        <location>
            <begin position="448"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="203"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="586"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="596"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="729" mass="81423" checksum="A25A1A687E8E6D4E" modified="2024-11-27" version="1">MDDSLQKSLSVLDRLLLTHSIWLQLSINPDSALCILQREIIGTFLVCKCASTQRKVLCVRVQESSVSVTQYPIREEDSTFSLESSALSFPDLCRLVAFYCISRDVLPFPLELPEAIAQATNHTQLETISHLGLDFWSSQTSPGPPNGPPAVSVRRTSRAGSPCFINPLFLQPPQPSRGASHKRHRFKRSLRVRVSTESSFCLSSAEPEWSDSDRLRADPGGSRRAGGLSMLRRTPALVPTSEEEDEHMLGEMPPDTSMPQQEEMQEMVQEEGVCLALERRRAPSLAELDSSSSFSSLEEEDSQLDASLQWPPAARGSRFPVANPASTLRRMSAAFVCVFAPERRVARLVDELSRDRRSVFGNLVQEFLEKQKEEMKAPGWSSAMQLLQGIRRFLSQAKSLLLEAGELEPPIETLVPENEKDLALEKALFGCVLKPLKLQLGQTLLSLHTQDGSLQRFANSLQACQQGTLERLGVRAGVLDAQGIERAKKKLTLMQRSHSPIDKVLLLLQVCKSVYKAMGTQPGQDYSSEEFLPVLSYVVVQCNIPQLLLETEYMMELLEPSWLTGEGGYYLTSIYASVCLIQSQPGTPSTSGLTNEARESLREWSRRRGQEAKIQRESQQTQRFVRVLFQNTEQSAARTLHWKAGESVEMLTQTCAGAFGVSDPQHYCLFWRNGGEMQPLPLHTQPHELGTHTEGAATLSYLRKDHDFSKMRRLTRGGAVDIEESVCEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KTQ2</accession>
    <name>A0AAR2KTQ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">RXFP1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KTQ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062775.1">
        <property type="protein sequence ID" value="ENSPNAP00000065869.1"/>
        <property type="gene ID" value="ENSPNAG00000031444.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286925"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="761" mass="88576" checksum="BD034C62284CF85C" modified="2024-11-27" version="1">MPALHHIYEHNVIPNSWKTASISLILKKDKNPSDCSSYRPISLLNVDFKIVAKVLARRLDALLPRLIHPDQTGFVRSRHGSDNVRRALNIVDHLSRNPDPAFIVSLDAEKAFDRVHWDFLFAVLKKINLGSSFINMIKSLYSYPNAVVNTNNFHSDVFPVSRGCRQGCPLSPLLFCLVIEPLAQSIRNSTRLTGIKIGGEDHRISLYADDVLLYLSNPNASVNIVLDLISDYSSVSGYKINLDKSLALPINISQSDNIRQITPFHISDTGFKYLGIVITPNLNNLFTTNYTPLWDKIEQDLRVWTALPNSFFGRINVIKMNVLPRLNYFFRTLPCYIPASFFKNINSRITEYIWNKKKPRIRFSILTKTKNQGGVSLPNFQLYYWSAQIIQMLSWFLDRHDSKWVEIESLSCIPLELKSLPFINNLEKCHSLKDKYTVYNTLLAWKDVRQYLKIPKNISLYSPICHNPDFPSQLRSIGLHTWVKSGIRDLSCIYGQSLMSFEQITEQFKVPKSDFYKYLQVRHFISSKETKLRTELNDMENVLIKPNPRLCKISYVYSTFFDVNEPCYNPLRQAWEKDLSIKFDDDSWNNMHKKIFPSCTSINIQEQNFKFFFRSYLTPSRLYKMCKNPSPLCPKCNSDTGTFMHMFWECTKIQVFWKDVHQVIQKMLEKHFHMSPCLYLLAHDPNGVLNGNQSYLMGSLSYLAKKCILLLWITPTVPTVKMWLTQLLNVFPLEKLTFEMHNRQQKFSEIWSPVWKVLEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KYB5</accession>
    <name>A0AAR2KYB5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucose(out) = D-glucose(in)</text>
            <dbReference type="Rhea" id="RHEA:60376"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KYB5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073275.1">
        <property type="protein sequence ID" value="ENSPNAP00000069343.1"/>
        <property type="gene ID" value="ENSPNAG00000027725.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160313"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032868">
        <property type="term" value="P:response to insulin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17431">
        <property type="entry name" value="MFS_GLUT_Class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000040">
        <property type="entry name" value="Solute carrier family 2, facilitated glucose transporter member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF91">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="370"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="22"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="477"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="555" mass="60157" checksum="03E11327E53D9EDD" modified="2024-11-27" version="1">CKGETNSILMPLKQVTCYLLFSLSTAVIGSLQFGYNTGVINAPEQKLQAFFNETWVKRYGKPIEPGVSIFVWSLAVAIFSVGGMVGSFSVGVVADKFGRRKSMFLVNILALIGGGLMGLCTLCSSFEMVIAGRLVIGLFCGLFTGLTPMYVGEVSPTPLRGAFGTLHQLGVVLGILISQIFGLEFLLGSDKLWPLLLALTVVPAVIQCVLLPFCPESPRYLLINLNQEEEARKALVRLRGFEDVSKDMQEMKEESVKMNMEKKVTIPELFRTATYRQPLLIAVVLQLSQQLSGINAVFYYSTGIFESAGVTQPIYATIGAGAVNVVFTVVSLFLVERAGRRSLHLIGLGGMAVSALLMTIALLLKHIESLSYLSIGAVFAFVAMFEMGPGPIPWFIVAELFSQGPRPAAMAIAGFSNWTANFFVGISFPKLEKLCGPYVFIIFMIFLIFFFIFTFFRVPETKGRTFEDIAREFRGASPPAATSVQEAGGDMASTSPVKEKVPLVAANSGAEEKPSSSVKATSEATKGEEKPESATEPLVKSTTEEKSNSTVQESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KZT5</accession>
    <name>A0AAR2KZT5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Protein inhibitor of activated STAT protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KZT5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084347.1">
        <property type="protein sequence ID" value="ENSPNAP00000067892.1"/>
        <property type="gene ID" value="ENSPNAG00000017645.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234539"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16821">
        <property type="entry name" value="SP-RING_PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000003">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000009">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.780:FF:000002">
        <property type="entry name" value="E3 SUMO-protein ligase PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.780">
        <property type="entry name" value="PINIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023321">
        <property type="entry name" value="PINIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038654">
        <property type="entry name" value="PINIT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF9">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51466">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="20">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="PINIT" evidence="22">
        <location>
            <begin position="106"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="21">
        <location>
            <begin position="298"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="396"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="396"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="444"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="494"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="506" mass="55959" checksum="8F418C80618FFA17" modified="2024-11-27" version="1">EDLSFVSAMRNMVKSFRVSDLQTLLASMGRSKSGLKQDLVGRALRLVQTEYSPELLKNVRQLYETRFPKASGWLAARRPEGVPAVSYATLNTSPRGTTQGTDYLNGMPKLAAPPAAEVKLVPLPFYHNLETLLPPTELIAQNSEKLQDSQCVFELTQNQVDQIRNSSELRPGVKSVQVVLRICYTDSIGVQEDQYPPNIAVKVNQSYCHVPGYYPSNKPGVEPRRPCRPINITPWLHLSTATNRVTITWGNFGKRYSVAVYLVRVFTSAELFNQLKHCSVESAERCRERIQDKLRFDPESEIATTGLRVSLICPLVKMRLGVPCRALTCAHLQCFDAVFFLQMNEKKPTWTCPVCDKPAPFELLTIDGLLSEILKETEDVEEIEYLTDGSWRPIRDDKEKERERENNHTPDYPVVDICVPEANGHSPAHSSTSQSGKSAPGIQAAATGGAGATASSGAVVDLTLDDSSDEEGGGGAGDSEDTEDSQDSPAPKRGRYDYDKDLVTAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L0M1</accession>
    <name>A0AAR2L0M1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like DNA-binding domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MSANTD4</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017538734.1">
        <property type="nucleotide sequence ID" value="XM_017683245.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017538736.1">
        <property type="nucleotide sequence ID" value="XM_017683247.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017538737.1">
        <property type="nucleotide sequence ID" value="XM_017683248.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017538738.1">
        <property type="nucleotide sequence ID" value="XM_017683249.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017538739.1">
        <property type="nucleotide sequence ID" value="XM_017683250.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L0M1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060433.1">
        <property type="protein sequence ID" value="ENSPNAP00000070055.1"/>
        <property type="gene ID" value="ENSPNAG00000031068.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411603"/>
    <dbReference type="CTD" id="84437"/>
    <dbReference type="GeneTree" id="ENSGT00440000039469"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026162">
        <property type="entry name" value="MSANTD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21732">
        <property type="entry name" value="MYB/SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21732:SF0">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="5">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="217"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="315"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="250"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="417" mass="47299" checksum="89693D73EE6E3026" modified="2024-11-27" version="1">MDFLQMKHLKRKRKSNYSVRETQTLIREIQRRRDVLFSRQQNTAVNELKRRAWEEVAECVNALGEGEVRTAGEVKRRYLDWRTLMKRKQLRAQLAAGLQLDYDPPCSPDNEGSLGGELPQDPAGFQKDQGYWQDLPDLGENSVHCGVKLEDTEGSGYNLDGGVCDSVPGEVEGEGDEDDEDCFPSILQDVDREGTIPEDFAHIDEFGVLSCSKAPTGPSLARDLGPDTPGAPEVGGATPGPSAGDSTGLLVALERQRLELEKQRLQVESERLQVEKERLQVEKKRLCQAEMERERLQLEKERLDLERERLRLLVHQSALPQQSPASSTTTATPTTSSAPSSSSSMDVEKDRKPSWPVWTDLEAEKLKLEKERLLLEKERLQFFRFESGRLQIERERLQVEKERLQLHKDGQQMALHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L1V8</accession>
    <name>A0AAR2L1V8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ferroxidase</fullName>
            <ecNumber evidence="4">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L1V8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066764.1">
        <property type="protein sequence ID" value="ENSPNAP00000068371.1"/>
        <property type="gene ID" value="ENSPNAG00000025533.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158517"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000028">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000002">
        <property type="entry name" value="Hephaestin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF233">
        <property type="entry name" value="FERROXIDASE HEPHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="17">
        <location>
            <begin position="59"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="224"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="894"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1017" mass="114735" checksum="75E116A67B9A7353" modified="2024-11-27" version="1">MVEYTWNHCISGEQDLLHASVFLLQGLHRIGHVYKKAMFRQYTDGSYTKEIPKPAWLGYLGPVLRAEVGDVIEIHLKNFASRSYSMHPHGVFYKKNSEGALYPDGTSGALKQDDGVPPGGTHTYTWTVKPEYTPTEGDSNCLTWAYHSHVVASRDISSGLIGALLTCKKGANYSEVRRTDVDQDFLLMFSVVDENLSWYLEENIQTFCSDPTGVDPNDPDFQRSNKMNAINGYVYGNLPGIELCQNRTVSWHLLGMGNEVDVHSAYFHGHTLLMRGHRVDAVSLFPATFLTAEMTPVETGTWLLNCQVNSHIQDGMQALFRVSPCGLQDDSQAPLNGTVREYFIAAELVQWNYAPSGMDTLTNVSLTESQSPSEVFFGTRGGRLGGKYRKAVFRAYTDHTFTSMKEQTSMETHLGILGPVLRAEVGDILLVTFLNNADRNYSMQPHGLQYGKEFEGAEYADVTDTAFLLCCSSGSPFPLFKYYPGTVKNGSHVQPGDSFTYRWQVREGPSESDPPCISYLYYSSSDPIRDTNTGLIGPLLVCKRGALSSNMTQSFLFHSKISVNGYMYGNLPQLEMCEGARVKWHLLGLGTEVDVHTVHFQGNTFHREGTTRDTLTLFPHTSLSVFMQPDTAGLFEVSCRVSEHYMGGMRLQYRVKRCRESLSAPITAPTGHYFIAAEEVEWDYSPERTWELLAHLFTLNPGSIFVGKGENRLGSKYKKVVYREYTDATFRTRKQQQPAEKHLEILGPIIRAEVGEVLQITFLNKATRPYSVQPHGVKTIPTHPQPVLPGNMSRYQWIVPERSGPGISDPNCISFAYYSAVDFIKDTVSGLIGPLVICRKGTLNWDRQRLDIDREFALLFFIFDENKSWYLEQNIKTYYNNSLPLLRDGDFEESNKMHAINGKLYGNLHGLDMQKGERVNWYLLGMGSEVDMHTVHLHGQTFIYRTDLAHRADVFDLFPSSFQTLEIMADSEGTWLLHCHVADHILAGMETTYTIQPRADPCSSVPKFIHWFIVQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L281</accession>
    <name>A0AAR2L281_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dipeptidyl-peptidase 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Invadopodium membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L281"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054615.1">
        <property type="protein sequence ID" value="ENSPNAP00000068481.1"/>
        <property type="gene ID" value="ENSPNAG00000029479.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163944"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040522">
        <property type="entry name" value="DPPIV_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF205">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18811">
        <property type="entry name" value="DPPIV_rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidyl peptidase 4 low complexity region" evidence="9">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="8">
        <location>
            <begin position="124"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="7">
        <location>
            <begin position="551"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="751" mass="85792" checksum="1485B76D0D09D072" modified="2024-11-27" version="1">MFTRVSVHFNAHSNAFPPLTLQNVWKIVLAVLAVAVVVILIAVPTALFLNEKKEDPRRTFTLNDYFNSTIRARSFNLRWLSDNEYLHKTQEGSVLLFTVPSENGQEFLSRQPFERVEAFDYMVSPDRKFVCFLSNYTKLWRHSYMASYSIYDRENGTFITADIPQDVQYLAWSPTGHKLAYVWMYNVYVKDSPTTPSQQVTFNGAHNRILNGIPDWVYEEEMFSTNHALWWSPTGRYLAFAEFNDTNVHTIEYSWYGEGQYPDMVVIPYPKAGTPNPTVRLFVADATKVSRIMEVVVPSAVGEGEHYLSTVTWATDDRIAVQWQNRTQSYLTLQLYELNGDTWRETTRRSVSSSTGWVGRFSPDDPVFHPSGSTFYYIMSDSNGFKHIHHVSEVSSFFTAAALTYYVSNEHNGSPGQRNVYRVSIEGSTHSQPVCLTCTVNEDRCQYNSAYFSTEGTYYRMDCSGPGLPLYTLRDSRGAEIRVLEDNKNLDSVLQDIAMPTVTHGTMRIGEFELWYQMTLPPNFDQSKKYPLLIDVYAGPCSQKSDFRFRINWSTYLASTENVIVASFDGRGSGYQGDKIMHSIYKRLGTYEVEDQITAARHFINLGYVDKEKMAIWGWSYGGYVTSMVMGAGSGVFKCGMAVAPVSKWEYYDSIYTERYMLTPAENQAFYDNSTVTGRAKNFKTVQYLLVHGTADDNVHFQQAAQISKALVDEQVDFDSMWYTDEDHSLGGSANQHVYTHLSHFLKSCFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L3X9</accession>
    <name>A0AAR2L3X9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L3X9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081319.1">
        <property type="protein sequence ID" value="ENSPNAP00000069081.1"/>
        <property type="gene ID" value="ENSPNAG00000030940.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286930"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000002">
        <property type="entry name" value="Gap junction protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF49">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="161"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="309" mass="35477" checksum="3CC27DA0ABD8516F" modified="2024-11-27" version="1">MGDWSSLGKLLEKAQEHSTVVGKVWLTVLFIFRILVLGAAAEKVWGDEQSGFTCDTKQPGCQNVCYDKTFPISHIRFWVMQIIFVSLPTLIYLGHILHLVRMEEKQKQKRERENSEFSEKQVLIQAGKAKKPPVLDEKGRIHLQGALLRTYVLNVIFKTLMEVGFLVAQYFLYGFQLKPMYTCSSWPCPNTVNCYISRPTEKTIFILFMLAVACVSLLLNLVEMYYLGFTKCRQGLRYRCSHARKHTELPSDNTVVPFVASFPYFPSHPDLSRQPHSNTASKQNKQLGRTILTEPHHNHNNFAADRLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L829</accession>
    <name>A0AAR2L829_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L829"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047245.1">
        <property type="protein sequence ID" value="ENSPNAP00000070526.1"/>
        <property type="gene ID" value="ENSPNAG00000024333.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175037"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF11">
        <property type="entry name" value="GALECTIN 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043669609" description="Ig-like domain-containing protein" evidence="12">
        <location>
            <begin position="24"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="28"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="196" mass="22102" checksum="312A3FD593219956" modified="2024-11-27" version="1">MTLNTRTSLLYTTVILCIATCAGSLPAPSRIFMSARVGSSAVLPCDWRNVSTQSPHVEWRTYTETVFERRGEELYQGEGYKERVDVPEDHLLKGDCSLELKNVRPEDAGVYESFLLVRRTKRSLRTKRVFLQSVELSVDDAPEESHSENKAAACSAGTIQTSSWITAIFLLFFLLFTSFHLSVDHHLDVVERLAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LEA2</accession>
    <name>A0AAR2LEA2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 423</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ZNF423</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000044666.1">
        <property type="protein sequence ID" value="ENSPNAP00000073034.1"/>
        <property type="gene ID" value="ENSPNAG00000015599.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158492"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000468">
        <property type="entry name" value="B-cell lymphoma 6 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001049">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000244">
        <property type="entry name" value="zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000483">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000548">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000760">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004063">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000107">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000143">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000167">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000261">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000326">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF128">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="358"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="595"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="715"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="776"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="804"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="834"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="871"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="897"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="994"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1040"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1069"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1241"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1289"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1319"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1350"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="1380"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="64"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="406"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="929"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1219"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="426"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1219"/>
            <end position="1228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1405" mass="155432" checksum="B9B7283D1E36C453" modified="2024-11-27" version="1">MDGGREGWRELPLWEISIVHHFTSGRPIPIAPPLSVCVCALNCPKIANQNTAGFSVDEAESAEFTSSWDSSAPADVAVPERDSEGKDRAGGEDGEHSVTSHDERAGEEDLDDESIFTCDNCQQDFECLADLTEHRTNHCPADGDDDPGLSWVASSPSSKDVASPSQMLGDGCCDLGMGTGEEEGGSGLPYPCQFCDKSFSRLSYLKRHEQIHSDKLPFKCTFCSRLFKHKRSRDRHVKLHTGDKKYSCQECEAAFSRSDHLKIHLKTHSSSKPFKCSVCKRGFSSTSSLQSHMQAHRKNKEHMAKKDQGKRDGSTGDAADQDQDLYMCDYCEETFSQTDELEKHVLTRHPQLSDRAELQCIHCPEIFSDEGTLLTHIDRTHANKKHKCPMCAEQFSSVEDVYCHLDSHRQPDSSNHSASPDPVLGSVASMSSATPDSSASLERGSTPDSTLKPAQGRRKLAPSSDHDEGGWSGKVTYSCPYCSKRDFNSLAVLEIHLKTIHADKPQQSHTCQLCLDTLPTLYNLNEHVRKAHRSSGGSSSNFPILQFSNVSAFHCNYCPDMFADINSLQEHIRVSHCLPGGVVAGSTTLEGNHAFFCNQCSMGFLTESSLTEHIQQTHCNTGGGVTKMESPVLQPSQSFMEVYSCPYCTNSPIFGSLLKLTKHIKENHKNIPLANNKRKVKVADLSPASSDVEISSPKRHRVASDATQAGANGDYPCNQCDLRFTSFEGFQTHLKSHLELLLRRQSCPQCNKEDFDSQESLLQHLTIHYTTTSTQYVCESCDKQFSSVDDLQKHLLDMHTFVLYHCTLCQEVFDSKVSIQVHLAVKHSNEKKMYRCTACAWDFRKESDLQTHVKHTHLNQPTGPGVAGKARKCIFCGESFGTEVELQCHITTHSKKFNCRFCGKAFHAIVLLERHLREKHCMFDGGNGNGNGGSQNGTPNGVTQSSKRGGGGSTAAAEQADLQNMLLKGGTQETANSHEASGGEEEMDASEPMYACDICGAAYTMESLLQNHRLRDHNIRPGEDDAGSRKKKADFIKGNHKCNVCSRTFFSENGLREHAQTHRGPAKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGTCRICKMPLQSEEEFIEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKLSSSGGSGGGGGSASSSPNGQLQAHKLYKCALCLKEFKNKQELVKLDVNGLPFGLCASCMSRGTNGQSSSGVVTPQEPGEKGTSGLRCPECAVKFETLEDLESHVQVDHPEISPETSAAKKVTDASPVPKKKTYQCIKCQMTFETEREIQIHVANHMIEEGINHECKLCNQMFDSPAKLLCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFAVHGQEDKIYDCSQCPQKFFFQTELQNHTLSQHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LFL1</accession>
    <name>A0AAR2LFL1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ran-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with CHC1 in a Ran-stimulated manner. Interacts with XPO1. Interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 and MH2 domains); the interaction results in the nuclear export of SMAD2 and termination of the TGF-beta signaling. Interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain); the interaction results in the nuclear export of SMAD3 and termination of the TGF-beta signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017574007.1">
        <property type="nucleotide sequence ID" value="XM_017718518.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LFL1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043209.1">
        <property type="protein sequence ID" value="ENSPNAP00000073494.1"/>
        <property type="gene ID" value="ENSPNAG00000027857.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439867"/>
    <dbReference type="CTD" id="393663"/>
    <dbReference type="GeneTree" id="ENSGT00940000158588"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13180">
        <property type="entry name" value="RanBD_RanBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000106">
        <property type="entry name" value="ran-binding protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF142">
        <property type="entry name" value="RAN-BINDING PROTEIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RanBD1" evidence="13">
        <location>
            <begin position="335"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="275"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="462"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="57"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="482"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="492"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="529" mass="56653" checksum="0EBCD21E94341BD6" modified="2024-11-27" version="1">MADLANEEKPAIAPPVFVFQKDKAQKRSAEGSSAEDGEDSDREDGEYCPPVKRERTSSLTQFPPSHSVSKNNVFMPSSFCQSPTGNSDSEPEEKPVGFRLKPPTLIHGQAPSAGVPSQKPKEQQRSVLRPAVLQAPPTKVLTEPSKSNASCGTNGVGKFSDAATEGRSIFLNNTDSSDKSPKKDEGGENGGGKTEESAQRKDAVVDSGFVFGQNIKERAKLDESSTVSESKDSTQDSQSGSTNYFLQYMGTPSSLNATNNTEKGAKFVFGQNMSERVLSPPKGGESTPEGGKDTASVSEPSSQETTPEKNHSVTESLEESAAAYTKATAKKCILEKVEVRTGEEAESNVLQMQCKLFVFDKPAQSWIERGRGLLRLNDMASTDDGTLQSRLVMRTQGSLRLILNTKLWAQMQVDKASEKSVRITAMDTEDQGVKVFLISASSKDAGQLAAALHHRILALRSRAEQEPESTPVPPQTEIPQSNEEDSDDDELANNNNNNSSSSSSITTAGNTSASGTSETSGEPPAAEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LGL0</accession>
    <name>A0AAR2LGL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LGL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064013.1">
        <property type="protein sequence ID" value="ENSPNAP00000073511.1"/>
        <property type="gene ID" value="ENSPNAG00000037924.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230358"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002397">
        <property type="entry name" value="Immunoglobulin heavy variable 4-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="105" mass="11766" checksum="7297A5CFCB22793B" modified="2024-11-27" version="1">QSMESLETAVQKKPGEALSLSCRGSGFNFGGYGMHWVRQQAGKPLEWIGVIWYDASKTIYAKPVEGRIEITRQNSQNMVYLKLTGLTEQDSAVYYCARNHSGTNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIS9</accession>
    <name>A0AAR2LIS9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000062598.1">
        <property type="protein sequence ID" value="ENSPNAP00000074246.1"/>
        <property type="gene ID" value="ENSPNAG00000005433.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286928"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12792">
        <property type="entry name" value="RasGAP_plexin_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000203">
        <property type="entry name" value="Plexin B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000798">
        <property type="entry name" value="Plexin B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000102">
        <property type="entry name" value="Plexin B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000035">
        <property type="entry name" value="Plexin b2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000042">
        <property type="entry name" value="Plexin b2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000812">
        <property type="entry name" value="Plexin B2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057533">
        <property type="entry name" value="PSI_Plexin-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF9">
        <property type="entry name" value="PLEXIN-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24317">
        <property type="entry name" value="PSI_Plexin-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24479">
        <property type="entry name" value="PSI_PlexinA-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Sema" evidence="15">
        <location>
            <begin position="31"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1261"/>
            <end position="1288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1881" mass="212371" checksum="0026F4650CD67C6E" modified="2024-11-27" version="1">MSSCGLQMSHQRRTNKEMAGWVQMVRWVQMALFLVWSVSAESVSGEFLEFSTDMPINNVVQDPATGLLYLGAVNAIYQLDASLKLEARAVTGPKKDARSCTPPVSTCVTTELKDTNNINKLLLVHPTNRSLIVCGSVYRGICSLLNLNNVNEQIYYSDSKGERTYAASIDENVSVVGVMATFQLKDDPDKKPFDVFLIGKGYGGHEDNPKLITTRILENYKEWVVFESIVEASPVQAIPYPPLYQHQFRFAFKDTGFVYFLFSRTLGGAENFTFVSRLCEKDEGYYSYTELQLSCRKNGVQYNKVQAAYITEPGKELARAMQDSGQYGTVTVWDKVLFIVASRDGEDATDSALCMYPLKRINERVEDILNACYTENGKIGGQQAVYSPYKSITDNPCENRHSKDIVEQNRCGAEFLRYPLASRPQFALQAEAIFTRKGHGHLTAVAIAVENDHTIAFLGNSLGEVVKVHLSGNSEVSIRVPGDTMGSRVNKNLFFDKTQSHLYITTEKKVTKVPVQLCHLKTDCMSCMAQKDPYCGWCVLEGRCTRRSECSRAEEKNGWLWSSRKECVKIVSFHPPNLSCKKSQQVEINIPSLPSIRDFDRIQCRINSFQSKAVMVDSMVRCLLPPPYELPATPPHQDFVAVPLKVLVNDSVEVASRNFEFYNCAAAIRKAENTPCTACVSRLWACQWNTADHTCSDLNDAETGPNIIKHKQAKNCPRFENPDPILIPVGHKTRISFEGVNLEAYKDREFTIGTELMKQFEDDVKAEDGPFYSFSGYMFSYDKTQEASILFHIKEKNTDKKIDSTLNVTLYNCSLGREDCSLCKNADLKYQCVWCPQTRSCVFEKLCSTVELQECPDPGISAIIPRYGPLNGGISVTIKGSNLGIQKEDIKHITVAGVPCIQQEEKYSVSTSVVCEIGEVDPKGPLEGAVEVEVKGGKKGTSSVFFTYRDPKPLAVYPDKGPKEGGTLITITGNQLHTASKEDIAITVGGVPCQVEGFGEQITCKVGKYPSEKVPSDVLPVKVHYGKRTETQVSAAFQFSNNPVITDYQPRDSFDCGGRRIMVMGSGFDLIQKATMRVMVTADEGPVQEFVEEAVSKNDSMLLFLSPAVNRTSDSQMITSIHLDSYTKELKPFEYHTNPIFYKLSKDVIIENSVFIVTGRGFSKAMTAKEAQAFLGDVSCNVNTLQDDKLFLDPPTTTPRSRSKRQRRDVTSELLDLLIKFGNGEWVVGSVRYERKYEVKLDKIIIPAVVVPMLLIIAISIVCYRRKSQQAEREYEKVKHQLENLEESVRDRCKKEFTDLMIEMEDHTNDLSEGRIPFLDYKTYTDRVFFLPSKDGANDVMITGKLDIPEARKATVAQALNQFSNLLNSKTFLITFIRTLEARSDFNARAKVYFASLLTVALHGKLEYYTDIMRTLLLGLMEEYVQSKNPKLMLRRSETVVERMLCNWMSICLYQFLKDSAGEPLYKLFRAIKHQIEKGPVDSQVKKAKYTLNDTGLLGDDVEYSMLTLQVLVQGEGPDITPVKVLDCDTISQVKEKIIEQVYKNLPYSQRPKVESVTLEWRPGSTGQILSDLDVTSQKEGRWRHLNTLAHYNVRDNATLVLSRVLHTQQSYDQNHDNHEERNALLEDDKVFHLVRPADELDEMKSKRGSIKDKSMTKAITEIYLTRLLSVKGTLQQFVDDFFRSVLCSGTVVPPAVKYFFDFLDEQALKHENVDEETIHIWKTNSLPLRFWVNILKNPHFIFDVHVTEVVDASLSVIAQTFMDACTKSEHKLSRDSPSNKLLYAKEISTYKKMVDEYYKGIRQMVPVSDQDMNTHLAEVSRAHTDKLNTQVALHQLYQYASKYYKEIVLSLDEDPAAQSKQLTLRLQQIAAALENKVTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJ11</accession>
    <name>A0AAR2LJ11_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJ11"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079825.1">
        <property type="protein sequence ID" value="ENSPNAP00000076535.1"/>
        <property type="gene ID" value="ENSPNAG00000035058.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="63" mass="6528" checksum="2F0DC4476D70097E" modified="2024-11-27" version="1">MNLSWHLEAGDTIRGLDPSSAVSLSALIGSANTQSAAPDTLPPLPKTQPSPCRPLGHVLPNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LMG2</accession>
    <name>A0AAR2LMG2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Delta(14)-sterol reductase TM7SF2</fullName>
            <shortName evidence="16">Delta-14-SR</shortName>
            <ecNumber evidence="16">1.3.1.70</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">3-beta-hydroxysterol Delta (14)-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Sterol C14-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Transmembrane 7 superfamily member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TM7SF2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP(+) = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:18364"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Steroid biosynthesis; cholesterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Endoplasmic reticulum membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Microsome membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.70" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LMG2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070357.1">
        <property type="protein sequence ID" value="ENSPNAP00000077758.1"/>
        <property type="gene ID" value="ENSPNAG00000028182.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000417"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="5 16" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="11 16" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="14 16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="469" mass="51727" checksum="81E7D39BAE23AB63" modified="2024-11-27" version="1">MKLNKHKPAQHTEMEFGGALGAACIPVFLPLTVLYLLSVCRTPGGALLQWPPPLPHSTFLWDPVAAAIMVGWISLQSALYLLPMGKVSEGLVLRDGSKLKYPINGFHALCMTAVLLAVCVCLGMPLGYLFDLVLPLAVCAIGVSYLLALYLYIRSFWAPAHQLALGGNTGNPLYDFFIGRELNPRIGSFDLKFFCELRPGLIGWVVLNAAMLMKEVELRGSSSLAMLLVNGFQLLYVTDALWNEEAVLTTMDIVHDGFGFMLAFGDLAWVPFTYGLQAMFLVIHPQTLSPLSALGIILLNGVGYSIFRKSNSQKNQFRRDPAHKSIAHLETIATATGKRLLVSGWWGFVRHPNYLGDILMALAWSLPCGEDTRKEGLAKDLIILFLLCFSRISRLHQHHLCVSVLQVFLTFCHISMSFIFPSCWSIGRRETSGNAEPNTAWPGTPTADGFPTGSSPTSTDHTWTIIYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LNN8</accession>
    <name>A0AAR2LNN8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homogentisate 1,2-dioxygenase</fullName>
            <ecNumber evidence="4">1.13.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Homogentisate oxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Homogentisic acid oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Homogentisicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">HGD</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the conversion of homogentisate to maleylacetoacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>homogentisate + O2 = 4-maleylacetoacetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15449"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:17105"/>
            <dbReference type="EC" id="1.13.11.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:15450"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homohexamer arranged as a dimer of trimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homogentisate dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.5" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LNN8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070213.1">
        <property type="protein sequence ID" value="ENSPNAP00000078175.1"/>
        <property type="gene ID" value="ENSPNAG00000033617.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004601"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004411">
        <property type="term" value="F:homogentisate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07000">
        <property type="entry name" value="cupin_HGO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000026">
        <property type="entry name" value="Homogentisate 1,2-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046451">
        <property type="entry name" value="HgmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046452">
        <property type="entry name" value="HgmA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01015">
        <property type="entry name" value="hmgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056:SF0">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20510">
        <property type="entry name" value="HgmA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10 19" id="KW-0408">Iron</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase N-terminal" evidence="21">
        <location>
            <begin position="39"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase C-terminal" evidence="20">
        <location>
            <begin position="289"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF20510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="460" mass="52726" checksum="FAA749EF7549207E" modified="2024-11-27" version="1">MQDAVLGLHCRMLDVRLYYYYYYYYYHHSFLLLVPDWLRPQVCPYGLYAEQLSGSAFTCPRATNKRSWLYRILPSVRHKPFTPINCGNLSENWNEVEPDPNQLRWMPFTVTKASEKKVDFVSGLHTICGAGDSKSRSGIAVHIYTCNTSMINRCFQNSDGDFLIVPQKGEILITTEFGKMMVEPNEICVIQQGMRFSVDVFGETRGYILEVYGAHFELPDLGPIGANGLANPRDFQIPVAWFEERTVASGYTIINKYQGKLFSCQQDFSPFNVVAWHGNYTPYKYNLENFMVINCVAFDHADPSIFTVLTAKSTRPGVAIADFVIFPPRWGVADRTFRPPYYHRNCMSEFMGLIKGHYEAKEEGFQPGGASLHSIMTPHGPDFDCFEKNSTAELKPERVAEGTMAFMFESSFSMAVTKWGLETCQRLDKSYYKCWEPLKSHFNPNWRPSSNHSLEPRNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LNY8</accession>
    <name>A0AAR2LNY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LNY8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045892.1">
        <property type="protein sequence ID" value="ENSPNAP00000076454.1"/>
        <property type="gene ID" value="ENSPNAG00000031992.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="108" mass="11928" checksum="130A8DCFD4F3CB50" modified="2024-11-27" version="1">MCVAAPTLTVLPPSSVELQQGKATLVCLANKGFPSDWRLSWKVDGRSWSSGVSQSPELLQKDDGLYSWSSTLTLQEDQWLKKTVTCEATKDSQPPVSQTLRREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LQB2</accession>
    <name>A0AAR2LQB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SYPL1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017542056.1">
        <property type="nucleotide sequence ID" value="XM_017686567.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LQB2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047093.1">
        <property type="protein sequence ID" value="ENSPNAP00000076496.1"/>
        <property type="gene ID" value="ENSPNAG00000036833.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413868"/>
    <dbReference type="CTD" id="6856"/>
    <dbReference type="GeneTree" id="ENSGT01030000234637"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF33">
        <property type="entry name" value="SYNAPTOPHYSIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00220">
        <property type="entry name" value="SYNAPTOPHYSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="11"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="239" mass="26334" checksum="6FA2B86371FCA968" modified="2024-11-27" version="1">MQTGFRFNLSPVKEPLGFIKVVEWLTAVFAFGSCGGYSGRNVISLFCSSGSNETLEAKFSYPFRLNQVRLVAENVTLCNHTVSETHLVGDSSSSAEFFVAVAVLAFLYCMAALLVYVGYMHVYRDSDFGPMFDFVVTAAFAFLWLVCSSAWARGLQLVKYATGNEGIASTLLPCREDTVTCDVTEFASMRTLNISVVFGFLNLIVWAGNAWFVYKETRWHSQKVNSQQAASRGPGPAPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LTP1</accession>
    <name>A0AAR2LTP1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LTP1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053251.1">
        <property type="protein sequence ID" value="ENSPNAP00000077696.1"/>
        <property type="gene ID" value="ENSPNAG00000002114.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162556"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001809">
        <property type="entry name" value="Si:ch1073-365p7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF107">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="171"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="495"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="524" mass="60226" checksum="698691C819D9419D" modified="2024-11-27" version="1">MSSEILKNIVFSLHFCNRGPHAVLLVIRLDILFKEKQLKAIEGLLKHLGERAWNRTVVLFTHGDWLGDTPIEQHIESEGKELQQLVEKCGNRYHVLNNENWSDDTQVTELLEKIEEMVAANSGRLFQMDRKILQEAEEKRRAEEERVKERMMKVQKNRRCNQLQMGSTKHLSKLRIVLLGYRTAGKSSAGNTILNREEFELKTAAQCVKRQREVAGRHITVVEAPGWKFNESEFSSEVLKNIMFSLHVCIPGPHALLLVICLKFGFKGKQLKTIKGYLKHLSERVWSHTIVLFTHGDCLGDTPIEQHIESEGEDLQWLVEKCGNRYHVLNNKNRSDDTQVTELLEKIEEMVAANSGQKVQMEDERQIIEEGMLEGKVQDQRKKELIRSDIEPQMMWLSEETWEKSVGGSTLDTSVKRFDIDRAPSPAPSDLSLKTDWSMDANLNFSDNQRFKLENRPSSVLSYLSVETEYSAEHSFSLSETELFQNQRFGLLRGSSPVPNEASMKTDSSMNKLQWITSTSETEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LX30</accession>
    <name>A0AAR2LX30_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552729.1">
        <property type="nucleotide sequence ID" value="XM_017697240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LX30"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045297.1">
        <property type="protein sequence ID" value="ENSPNAP00000078886.1"/>
        <property type="gene ID" value="ENSPNAG00000030411.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108427240"/>
    <dbReference type="GeneTree" id="ENSGT00940000163149"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF62">
        <property type="entry name" value="CRYGM2B PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="3"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="132"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="180" mass="22109" checksum="556FF0D56E0346AD" modified="2024-11-27" version="1">MMGKVIFYEDRNFMGRSYECSGDCSDMSSYMSRCHSCRVESGCWMVYDRPNYMGNQYFMRRGEYADYMSMWGFNNWIRSCRMIPMHRGSYRMRIYERENFMGQMYEMSDDCESFMDRYQWSSGCMSCHVMDGHWLMYEQPNYRGKMWYFRPGEYRSFREMMSGMSGMGFMSMRRIMDSWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LYA5</accession>
    <name>A0AAR2LYA5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LYA5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070801.1">
        <property type="protein sequence ID" value="ENSPNAP00000079729.1"/>
        <property type="gene ID" value="ENSPNAG00000033713.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193390"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12893">
        <property type="entry name" value="SPRY_PRY_TRIM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="86"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="266"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="193"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="470" mass="53846" checksum="02ABC57D8909A2EA" modified="2024-11-27" version="1">MTARNPLSEEELSCPVCCDIFRDPVLISCSHSVCKTCLQQFWVAKGSRECPVCRRRSSRELPPSNLVLKNVCESFLESRSQRSSAGSEGLCVLHNEKLKLFCLDDQQPVCLVCQTSKKHKSHDFCPVDEAVTEFKDKLKSALEPLHENLRFVFPHRLSFQTQAQHTERQIKEGFEKLHQFLRDEEAARIAALKEEEEQKGQMMRRKIEEMNGEISALSDTIRNIEKEMEAEDVLFLQFESDLCKRGLTPKDPEKTSGALINVGKHLSNLKFRVWENMQEVLQYTPVTLDPNTADPVLHLSDDLTAVENTKQRSSLPDNPERFDDGWCVLGSEGFNSGIHCWDVQVGDSDYWSLGVITESGTRKGDSFWGSVWRLNYDKSNNTIWIFCPGQSDHSFTPKEKLQRVRVQLDWDRGKVTFTDLLTSTHLHTITHTFTQRVLPLFYNYSVHPLRILPVKPSVTVEHTLPTPPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LYE0</accession>
    <name>A0AAR2LYE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transposase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LYE0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042614.1">
        <property type="protein sequence ID" value="ENSPNAP00000079682.1"/>
        <property type="gene ID" value="ENSPNAG00000033197.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286914"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057667">
        <property type="entry name" value="HTH_SB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25787">
        <property type="entry name" value="HTH_SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sleeping Beauty transposase HTH" evidence="3">
        <location>
            <begin position="37"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="104"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="184"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="43143" checksum="614C4D56B2AD6E1A" modified="2024-11-27" version="1">MTQKGPPVCNLISVQIQLLRDGLRCWLREYWGANSIMKSKEHTRQVRNMVLEKFKAGLGYKKISQALNISRSTVQSIIRKWKEYGTTVNLPRQGRPPKLTGRTRRSLIREAAKRPMVTLDEMQRATAQVGETVHRTTISRALHKCGLYGRVARRKPLLKENHKKSRLQFARSHVEVTANMWKKVLWSDETKIELFGHNAKRYAWRRNNTAHHSENTIPTVKYGGGSIMLWGCFSSAGTGKLVKVDGKMDGAKYRAILEENLLESAKDLRLGRRFTFQQDNDPKHKARATMEWFKKKNIHVLEWPSQSPDLNPIENLWQDLKTAVHKRSPSNLTELELFCKEEWARISVTRCAKLVETYPKRLAAVIAAKGGSTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZC2</accession>
    <name>A0AAR2LZC2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SH3TC2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSPNAT00000060743.1">
        <property type="protein sequence ID" value="ENSPNAP00000080002.1"/>
        <property type="gene ID" value="ENSPNAG00000006468.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063812"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901184">
        <property type="term" value="P:regulation of ERBB signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033157">
        <property type="term" value="P:regulation of intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042772">
        <property type="entry name" value="SH3TC1/SH3TC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22647:SF2">
        <property type="entry name" value="SH3 DOMAIN AND TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22647">
        <property type="entry name" value="SH3 DOMAIN AND TETRATRICOPEPTIDE REPEATS CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="225"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="315"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="1223"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="436"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1339"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1339"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1377"/>
            <end position="1392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1456" mass="165241" checksum="59CC03BF24E1D48B" modified="2024-11-27" version="1">MHDKITLSTNSILFKTVSQSCLNISTAELDALWNEPPYTLTGANELFPGNDVMATGEEEVETEAEVEGEGITGESYWKRKEAFNRASTVSLAVGERFSSDVILLFSGRRRSSAEPDSKLQEALRTRLRVVESNSQDVIQLFKDLSARLVSVHAEKDHFVITFKTVEEIWKFSTYLALGYVARCLENFLCDQSFWLDPVLLSDVEICVNVDEEHLATLYLGLLLQEGSFFAKALLNSECTEEEEELTYKKNDLVMVKDIGQEGTWEGTLLSTGQHGLVPVHSMQPLPYPFYQWFLRKYPGSTGGISSEKGLFDQPMVTGTCVAVVDHYPMVKDELHFSQGDLIEVDGLFISSLNMFIGRHLSSGHIGFVHKAHVRPENVKALSRQLVFLSEEERVALSQFNPCHEQCHTGLLDRLFSTDISTVYRLDRLDDSDFTYIRNQPKQEHKNPVDARKSTISEKSETPPYHSSPRPSFYASQSHLEREHEAFSFSLEDTFREMDEYEEDPPNYIDDNIWETEEAEFVDPILTLLNLDHFQDTFQPLYDISYSFLDTVFGGIPEDEVLLHLENVREGAKKGRMLWAHRRACFLLGRLCAKKLKYSQARVYFEEALKVPVDGFDDKLLLIALYTNLTAVYLKQKMTDKLPYTLEKASALILCLPCHNFCSLDEFELLKPIVRKAIVDNDKHLEARTCYLILCLFLQQRKIEEALPFVERLQFLTVTLSAEEGRPMGPVDLNWILCRLYHKKYLPYLALASLSLDSAQDHSLEDAFQKIELFIKNSVRLNPRWRDGTSLLPAQVVVYLQQALSIASHGEDLKTQRDLCLCLASVYQQYGALDKAVPFAHQAVQTRSHINEEESFEASVLLAWLLVLTGEPETAQSTLQPLLKSLNETDSPTQRGVVYNLLALCLRKQCRVTEATKNFYCALKISQENGNKHNEALALANMGCLALSVGAPCLAESFLLRSLHLFQFLSESPFDEEHVQALLWLGRSYKDRGGSQEVRLCYEIGLLIAMSAKNLHSQMVVAKVLSRLYADLLLYGQCIIYYEHCVGLSRELKDKRLEGEYLEILSSLYLSLNTEKSSRKSLDYTKQSLRISIDLGKRQEESETWLQVGRIYYLIHEDELADMYLQAAVKTALRMNDPRFAMSIYEEAGDVFFKGHRNRLAAMPFFRDGSLPFARSIKDVHSEFRLLSKLSELLLKENQYEEALQYATLAVQSSTDTGVRLNERVAYHRLASVYFCLEQYEMAENYFLKALSLSPTVLENTAEAHYYVKVYCRLAELTLHKLKDAFDAVGYFHLALAAALEDRGNPQALYIIYMKLAEIHANHLPDAELSQRYMDSAQSLKRELTGHTDSSEAEHENMDHGNPECADAKSVGYSNADSGSSGSSTLVGSTTTDTSHTITAQRLADHNEAAVADYSHQEDTDTCSEEMYSGPAHLTDAETSYFNQTNHRDASELNTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M2Q3</accession>
    <name>A0AAR2M2Q3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CKK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CAMSAP3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037393640.1">
        <property type="nucleotide sequence ID" value="XM_037537743.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000077222.1">
        <property type="protein sequence ID" value="ENSPNAP00000081289.1"/>
        <property type="gene ID" value="ENSPNAG00000016367.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429611"/>
    <dbReference type="GeneTree" id="ENSGT00950000182975"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000001">
        <property type="entry name" value="Calmodulin-regulated spectrin-associated protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031372">
        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF2">
        <property type="entry name" value="CALMODULIN-REGULATED SPECTRIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="202"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="9">
        <location>
            <begin position="1290"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="516"/>
            <end position="682"/>
        </location>
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        <location>
            <begin position="715"/>
            <end position="799"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1231"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="738"/>
            <end position="748"/>
        </location>
    </feature>
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        <location>
            <begin position="816"/>
            <end position="839"/>
        </location>
    </feature>
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        <location>
            <begin position="865"/>
            <end position="876"/>
        </location>
    </feature>
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        <location>
            <begin position="915"/>
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        </location>
    </feature>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00841"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51508"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1429" mass="157486" checksum="59769CAE8C6C06D6" modified="2024-11-27" version="1">MVDSSAMRKTFVVPDIKPLDQYDFSRARICCSVGWLLAKSYGNAENVPVELRDPFYCDQYEQEHLKPPVTRLLLSPDLYCRTYSLLLAGGSGTEAPPRDYAALLQSLSKKGLAPKDQNIPVTEADLSHKPIKMTAHLALIDVLMAVGAMETLSTVRASGGADLIKGEADWESALLHWVNTLNQKLKERTENDQTQPSTEPQPVQPSCPTRWYWKLVPLRYRKDKMLSKLKPCFPVVKEVTDLSNGCAIAAVIHYYCPDLLHLEDVCMKDSMSLADSLYNLQLIREFSDSCLKSCCHLVLEDMLYSPPELQVNILSFLAELLYWFEVSKPEFVQPLQAPELTEPCGRTDGGNSGTSNSSSPSIFKKPFLPISSPVTPAPGSLTQSTSMSHVEAAGQTWTKKPPSRSLSSAVSFSIPFGLDSDVDIVMGNPVITRSVSSDNLNPLGQPMTRVPYTPPEDLTHLLSKPSGPNGPHRASWATQSPKVPLLAEENGLDEGETGELPTIEEALQIIHNEGKMEPRLHPDGAPDGFYLHSPDDPSNRRHNGGPATLSCSAPSNSGMQHRPSGVTREPGRTRHTSEGSRDDDSVLRDGSVDSDASEELPKMHSTPAAPAGGARIVRPQGAETPDSGIKMTSFAERKKKVVPEQPTPSEEAAPQMTTWAAKKAEESPGKSPTLSNEMSELGARLEEKRKAIEAQKKRIEAIFAKHRQRLGKNAFLQLKKEESEEGGQGEIGTSSAELDERLARLESEERQDEEEEQKKQRSLLEDEGNASKQASIQQAPKEKPVAGTLGEKSTVPLGDYNNAVSKLNAALSSLQSDMQRLSEQQSQLMKKKGQPSNQAWVIPPSTKTSTPAPPRISRESTREMPPTSASSSPSPSRRITAQAMPPKSPGSHRRAQSAPPKSPKHHQPHSRSSDLKTPTLTRVITAPQSVDSIPHRRRLSPWQYRDQTSSSFSIGSPASQSESRPASQLFRPEDDNHSEAGSSEDRTIFSLDLEGGSTGQPMTRKEYLSGGGSSGAPSECSFESDVQPPSISGKRGSLIEISLSSLRGLEGEDTDHGPDAISDSMSDLTDPEMRPGVGFFFKDEARPEDEMAQRRAALLEKQQKRAEELKRKRQEQEREREASRSGSVDELRTADRVDQPQTPCTPPPARTPPPEGTPQRRGDFTRQEYERRQQLKIMEDLDKVLRQKPTTVRGVKKQRPKTVFHDDSALSRSPAKGFMGSKLNRVYSHSTNNLSSMANDGTLTVRKSPSRSHSPSRLMSPGRQAMHNGERDWEVASTISSPASIPEYTGPKLYKEPSFKSNKFIIHNAISRCCLAGKVNEPQKNKIIEEMEKSTANHFLILFRDTSCQFRAVYTMNPETEELVRLTGIGPRIISPSVIESIYKYSSDRKQFTAIPSKTMSMSVDAFTIPGHLWQSKRPGTPKKLGTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M2X3</accession>
    <name>A0AAR2M2X3_PYGNA</name>
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        <recommendedName>
            <fullName evidence="4">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M2X3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070166.1">
        <property type="protein sequence ID" value="ENSPNAP00000080986.1"/>
        <property type="gene ID" value="ENSPNAG00000031640.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164735"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="48901" checksum="C37A207BA65ED6E4" modified="2024-11-27" version="1">MSDDAFALWAKRGVVSLSDLYIDGKFASFEQLTQRYNIPRIHFYRYLQLRNFVSSNLDSFPLSPSISLLESIYKHKAINTQAISIIYGLLDSHNYTSLELLKSKWEMDLGGTILEETWCKIIQGIFSSSICAKHAVIQFKIVHRLHWSKVRLSKIKAGLDPTCDRCGLAPASLLHMFWSCSKLYTFWNSIFETFSKVSGKAVEPSPFIALFGVVPIDSAPNRRDINMFAFCSLLARRLILFKWKDANPPTYNHWVREVLYHLKLEKIRYTLRGSTGKFFDIWQPFLSFVEDFEADSVLTSNGLAESSVKVVKGLMKKAHDGKEDFHHSPMIYRSAPLQNSLSPAQILMGRRLRTNLPMHENLLTPKGAHKIKIDKEKDKEKQKTATRQKSKETARIKAWRTSQDQRSCDRHLESAGFSAKRGGTTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BKG1</accession>
    <name>A0A3B4BKG1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ski oncogene</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Proto-oncogene c-Ski</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548357.1">
        <property type="nucleotide sequence ID" value="XM_017692868.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BKG1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000297"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014089.2">
        <property type="protein sequence ID" value="ENSPNAP00000000297.1"/>
        <property type="gene ID" value="ENSPNAG00000000179.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424681"/>
    <dbReference type="CTD" id="30113"/>
    <dbReference type="GeneTree" id="ENSGT00940000166894"/>
    <dbReference type="OMA" id="SIERCET"/>
    <dbReference type="OrthoDB" id="3938623at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21083">
        <property type="entry name" value="DHD_Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000002">
        <property type="entry name" value="Putative ski oncogene"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.20:FF:000002">
        <property type="entry name" value="SKI-like oncogene a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.20">
        <property type="entry name" value="Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014890">
        <property type="entry name" value="c-SKI_SMAD4-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047315">
        <property type="entry name" value="DHD_Ski"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003380">
        <property type="entry name" value="SKI/SNO/DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023216">
        <property type="entry name" value="Tscrpt_reg_SKI_SnoN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005:SF24">
        <property type="entry name" value="SKI ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005">
        <property type="entry name" value="SKI ONCOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08782">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01046">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="c-SKI SMAD4-binding" evidence="5">
        <location>
            <begin position="192"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="647"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="655"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="678" mass="76063" checksum="74E4E292ED3F0C1C" modified="2018-12-05" version="1">MDSAPSFQPPAGLQQTLRQFHLSSMRSLGGPAAFSARWQREAPFGKTGGNEPLPPGPTRSPPPPLLPGPLLVPPDRSTARCETVLERETISCFVVGGEKRLCLPQILNTVLRDFSLQQINAVCDELHVYCSRCTAEQLEILKVVGVLPFSAPSCGLITQTDAERLCNALLHGGSLPPRGDPGAVELASTEKSIPVYHECFGRCDGVFVPELYSNPGAACVQCADCGFMFPARRFVVHSHRRRENRTCHWGFDSANWRAYVLLDRDRAQPAEHSELQRRLDELKEKFQRAGKHTAPEITAYSPVPSKKSKQERSRSPSEDKEKQADWLQYLTASTNKDLKQLQFKQRPSAFRPWSPRVPSLERAVPSHKHERSPPKRPESLAPSVTVTSAGLSKDETPQATGYQPAAPLTLSSRPTKHWPSGESRSLSAARLHQPAKNDSDTDAEIEVDTCDEIPHAHSLSNGVAGSSPAEVCDVRLKVTGPSELEALRQTLYSDLNSREARERFLQEIIRMRVKQEEKLTAALQAKRSLQQELEFVRATKKGRLREAIEAKRNLRKEIERLRVECEKKVRDANDLCGRLKREVERERQLRVCDKGCESGRLRAKYSTQIEDLQVKLQQAEADREQLREELLKEREARQSLERAVLQLQQQLNQNTKQAHTQESQQPNGRKNGEAVGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BS21</accession>
    <name>A0A3B4BS21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FAM20 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">FAM20B</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017544080.1">
        <property type="nucleotide sequence ID" value="XM_017688591.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017544088.1">
        <property type="nucleotide sequence ID" value="XM_017688599.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BS21"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001259"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012469.2">
        <property type="protein sequence ID" value="ENSPNAP00000001259.1"/>
        <property type="gene ID" value="ENSPNAG00000000627.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415546"/>
    <dbReference type="CTD" id="9917"/>
    <dbReference type="GeneTree" id="ENSGT00950000182951"/>
    <dbReference type="OMA" id="AVWEDDM"/>
    <dbReference type="OrthoDB" id="8583677at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10470">
        <property type="entry name" value="FAM20B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF14">
        <property type="entry name" value="GLYCOSAMINOGLYCAN XYLOSYLKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017375161" description="FAM20 C-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="FAM20 C-terminal" evidence="10">
        <location>
            <begin position="190"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="222"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="409" mass="45800" checksum="4C4FBEB66C1994DA" modified="2018-12-05" version="1">MKLKQRVVVLCGVLLLLGLAKVFLLDGGEGSAASRRDLRAFRKMEASLLLAKGARLTHTLQSPWEVAAQWVGPREVYPDDTPELAAVLNALASARVERADVGYKGTQLKALLVLDGGQKVVFKPKRYNRDYVVEGEPYAGYDRHNAEIAAFHLDRILGFRRAPLVVGRFVNLRTEIKPVATDQLLSTFLMHGNNTCFYGKCYYCRESEPACAEGDIMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTAVFDYLIGNADRHHYESFQDDGGASMLILLDNAKSFGNPALDERSILAPLYQCCMVRVSTWNRLNLLKGGALSSAMRQATAHDPAYPVLTEPHLAALDRRLNGVLATVRQCMELQGAESTLIEDRMNLPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTI5</accession>
    <name>A0A3B4BTI5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Laminin G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CSPG4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570460.2">
        <property type="nucleotide sequence ID" value="XM_017714971.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000001856"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011439.2">
        <property type="protein sequence ID" value="ENSPNAP00000001856.2"/>
        <property type="gene ID" value="ENSPNAG00000008405.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437699"/>
    <dbReference type="GeneTree" id="ENSGT00940000154091"/>
    <dbReference type="OMA" id="PWPQGTT"/>
    <dbReference type="OrthoDB" id="9026019at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039005">
        <property type="entry name" value="CSPG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051561">
        <property type="entry name" value="FRAS1_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739:SF13">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16184">
        <property type="entry name" value="Cadherin_3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51854">
        <property type="entry name" value="CSPG"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043400035" description="Laminin G domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="2308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="2214"/>
            <end position="2240"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="9">
        <location>
            <begin position="24"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="9">
        <location>
            <begin position="205"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="433"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="554"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="663"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="776"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="892"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1014"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1122"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1235"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1349"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1466"/>
            <end position="1556"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1573"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1694"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="5">
        <location>
            <begin position="1825"/>
            <end position="1917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2248"/>
            <end position="2275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001856.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2308" mass="256336" checksum="A027F83FD791E8FC" modified="2024-11-27" version="2">MDNMRALRLLVCALLPLLCLPQTSHGDSFFGDSYCRIDAVQDLLSFHLSLQFKTSRRSGLLLLAAGNKDYLSLELYNGKLQARINMGSGEKVLTSSTGVQLNNLLDHQVSLTLKGNKLSMTVDNLFTTFTEMQDGEDLNIDTGVFLGGVRDLDVEYLSTAVPFFRGCMSNITFESRQFDILSSESRACYDTKEACSSEFEARDGEATSFISPDSFVSFPTWTRASTGSRNIEVLMKTTIEDALLLFHPGHQSDFIAMGITGGHLKGVVDVGSGMVVLNNLEVQLDDDQWHRIKMQIEPNTFAITVDSESVSLPLNGSENLDLVGNLYFGGIQGKMKEVFRDNYLSRMEEEITSESFIGCLGEIKVNQKDRSLQDALITKDVHVKCEGEDYDYSSYYDFETTTTTAPLRIRYVGVTEERHCFPTPDMPEIFRNVSKLLDVSPLLVPEGGEAIVDVTNLNPTFNLNAVGMRQSQIIFTLQADPWYGLVDMNINTRRTKKFTLLDVVNKKIKYLHDGNEKYNDQIQLEVVAFGSSLPECLKTPHQYMLPVEILPVNDIPQLSGGDISVTENGRTRLSSNLIKIVDSDNQCDELTVTVTSNPNPNGYLENTEYPGRSLSKFTCRQLKDGLIYYVHRGGSVAGLTLQVSDGHQISQSATFKLAVTAPQMSLVTNTGLLLTQGSDTSIGIQNLAVSATPRNGDVIFNVTQPLKLGELQIIADNGVPQRVSLFHQSDLEQNKLRYISTISSDLDNTETEYIHFNAHLGQFTLPDNVFIIKIMPDQVRMNKIVPLEITEGPKVITHENLEAVVKGKNINSASIKYVLWKHPTLGALMLQGRDLSEGNSFTQQDVENGHVSYRPTIRRAVDAEDHFQFKVSADDQYSRVYTYPIRIKADSNAPSLTNEKLVVLEGNENILNKEYLWVQTPRSTDFVYRIVQDPTHGRLIRDSPPGVPRFEGAVRVFSNEDLLLNRLIYQHDGSEINRDQFIFLVFEEAEGSTEGQEVLKGIFNIAVQSKNDHVPQRVINKPFNVVRNGQRLLTTDDIMFRDDDSDFNDTQLVYARGGILSGSIVSSADPSQPLYRFTQADLRDGKVLFVHKGADRERFQLQVSDGLHKTTALLQVQASEPYLHVVNNTMVVIDHGSTKTLDSSLLGTEFNTDIRNAAEIVYDITSPPSDGRIIVSGIEASRFTQKDLTKGVVSYEHSDQSLKSKDSFTFTVHTKGLSEEGIFRIKIFKQGYLSEPQVTANQGIIAYEREHSIIDSDHLRVEQADIVPTEMVFSIKEPPRLGHVVKMTSDSDSTATPVLDYIHSFTQADVNKGTILYVSASLQGTDWFTMDVSNGFTTVEDLRVQVDIVPRFIPIEVVNFTLREGGAVALSEEVLNVTHPFYRSVNIEFIVEEAPQHGEIKYLDKDEEGIVSFTWKDVQQGLIYYLHDSTETTEDSLTLFASAYEINRRSQTVTISITVIPVNDEPPMLQRNTGLEVLVGEESEITNRLLNTEDVDTPPEELVYSIEAPTNGMVALKESPDDSIDAFTQAQINNGEVIFIHEGSKSGGFTFTVTDGDHTSPLYHFTVTARQLTISVEAQGELLVFPGSRQVINRDILRAVTSEDGDEISYSLVRPPRLGRLIKPNQSGQFEEITKFTQTELDAGAVYYEHQMPSEPFWVVKDSVELELSAPPAARLNHTLPVTVSYYAANRNVSSQLWKNKGMSVVQGQSKVIDSSILDASNLLASLPKEKQAGQDIVFEVRKFPVQGRLTLRGANLPRDTPFFLQDDINKGELEYSHDDSGASSDSFSFRVRLNPHGRTPQAAPGAVVLEEIFFISITRRDSSPPKLERLDLLLEALQGSTTVLSKQYLNTIDQDSTPDEVRFTILKSPANGHIIYKDTGDRIRQFTQEEVNMGRVAFVSDGSLSDGFMEFTVSDGKHQTEPHTLHIGILAKKLTLSKVQEIQVKQGDDETPITEAMLKVSTGGAREEDILYKITNVPKYAAVMVDRQPTSAFTQKQIKEGRVSVRFVKSTSPSDSFAFVARSRAANVSSALNITVRPLANVAKDPVLPRGMTVLVDRKLLDATPLANKTRTSPTFSVTQQPKGARFVRRGGSADGQPVESFTQRDLDEGRVALEVQNTTGVPKGKGQSQSQGEDEARFLLKAHGVPPAECILSFKTAPYDPAGVYGATVLKVPPSVPTTDKSKSGDSDWTIIVGPTTASTGSHKAPVSRRNILWAILIPILVILILLLLAALLAYYLVRRNKTGKHNVQTATAKPKNGEVSQETFRKTDPANNISMTTLESKEADPELLQHCRTTNPALKKNQYWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTK1</accession>
    <name>A0A3B4BTK1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KLF4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BTK1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002977"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009516.2">
        <property type="protein sequence ID" value="ENSPNAP00000002977.2"/>
        <property type="gene ID" value="ENSPNAG00000009332.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156229"/>
    <dbReference type="OMA" id="LSTRDCH"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002977.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="288" mass="31190" checksum="671DAE3C21233521" modified="2024-11-27" version="2">MRQPPSDVERVALGGALLPSISTFSSGGARTRWKEEMSHLKHPCLLSGGSDHNPLLTMTKKEPEELDLLDYDFILSNSILQQQQQQQQQSHASQHQSDEDPGMVSHAPPTSPGPYSAYQGEALYPIPDLNDVSPSGGFVAELMRPELDPAYLQPPASLHGKFVVKTTMHMDYSQGVTVSKAAASSSDPSVLFACHRIKQECPSKCAVSRAMDPCGFGHAPSSSSSSLSSPDEHHAPSQALGHPLGFHPTQGYSAFHSQPPAPSMQYQGRSDLSRSEPEKSRLWPLAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BV21</accession>
    <name>A0A3B4BV21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription elongation factor Eaf N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">EAF2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560189.1">
        <property type="nucleotide sequence ID" value="XM_017704700.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BV21"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002426"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010448.2">
        <property type="protein sequence ID" value="ENSPNAP00000002426.1"/>
        <property type="gene ID" value="ENSPNAG00000008836.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431497"/>
    <dbReference type="CTD" id="55840"/>
    <dbReference type="GeneTree" id="ENSGT00390000017724"/>
    <dbReference type="OMA" id="TVMLPNI"/>
    <dbReference type="OrthoDB" id="125903at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032783">
        <property type="term" value="C:super elongation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003711">
        <property type="term" value="F:transcription elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027093">
        <property type="entry name" value="EAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019194">
        <property type="entry name" value="Tscrpt_elong_fac_Eaf_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15970:SF7">
        <property type="entry name" value="ELL-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15970">
        <property type="entry name" value="ELL-ASSOCIATED FACTOR EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09816">
        <property type="entry name" value="EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription elongation factor Eaf N-terminal" evidence="8">
        <location>
            <begin position="15"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="261" mass="28376" checksum="FAED1F6321B27C21" modified="2018-12-05" version="1">MNGTAYSNFDNQEHVLKLGETFEKQPKSAFHTVRYDFKPASIDTTCEGELEVGKGEQVTITLPNLEGSTAPVTVFKGSKRPYMKECILIVNHDTGEYRLEKLSSNIAVKKTRAEGSSKIQSRLEQQTNRLSQQMKSGSGGKAPANSKSSPLKEKMSPASPMDDIERELMAEARVMDQMSSGDSSSDSHSSSSSSSEDSSSSSDSEDETRPSLGDPQTKPAPTRSMPILTSQEHVEEEGGHLMNTLKSDLQLSESGSESDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVP4</accession>
    <name>A0A3B4BVP4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Apelin receptor b</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566733.1">
        <property type="nucleotide sequence ID" value="XM_017711244.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVP4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002574"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010206.2">
        <property type="protein sequence ID" value="ENSPNAP00000002574.2"/>
        <property type="gene ID" value="ENSPNAG00000001257.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435413"/>
    <dbReference type="CTD" id="565492"/>
    <dbReference type="GeneTree" id="ENSGT01130000278303"/>
    <dbReference type="OMA" id="LCYCCIG"/>
    <dbReference type="Proteomes" id="UP001501920">
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    <sequence length="354" mass="39569" checksum="1DE1406E78EC7F25" modified="2024-11-27" version="2">MDNSDDYYPDYEDLPNNSMCDYEEWAPSYSLIPVLYMLIFILGLSGNGIVIFTVWKAQGKRRAADVYIGNLALADLTFVVTLPLWAVYTALEYHWPFGAALCKISSYIVLLNMYASVFCLTCMSLDRYLAIVHSLTSSQLRTQGHMRASLAAIWIISGVLASPTLLFRNTMYDKDTNRTSCAMDFSLVITKPGQENLWIAGLSISSTALGFVLPLLAMMVCYGFIGSTITRHFNSLRKEDQRKRRLLKIITTLVVVFAACWMPFHVVKSMDALSYLGLAPDSCSLLTFLLLAHPYATCLAYVNSCLNPFLYAFFDLRFRSQCLCLLNLKKALHASPASSLSSQKTEAQSLATKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXP7</accession>
    <name>A0A3B4BXP7_PYGNA</name>
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        <recommendedName>
            <fullName evidence="13">Seizure related 6 homolog b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="entry name" value="Sperma_CUB_dom_sf"/>
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        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
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        <property type="entry name" value="SEIZURE PROTEIN 6 HOMOLOG"/>
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    </feature>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1034" mass="113610" checksum="3917257442ADE033" modified="2018-12-05" version="1">MITVALAVLAVVVAARGQGSFMEGKMAVTQQGGSPEAENVQNAFHSTQDMDRELPLVTTAPPFAGLNHHPPFKAFPRPDSHHQGPQEFEDRLRVEPHLFKGPSTQLVPHEDAAPFFDVAATSPTTASLSMPPATSTAVQQQVGSRQLSGTPGEKIQRRSFEEPSAALITESLTTTVMPTSAPPPCHTAREKQPGHVDMATAQEPQAVTTLVTREEEEAPKTRAQHDTDTTAVSRDPDDETTTTTIITTTVITTMQTPVPCSTNFTATGGYIETPPPSSWYYSNVDCTYTVTVYMGYGVEIQVMNVSLAEGDSVRFEDLGGRQPSVLANESILMKGLVVRSYSNQISIHFHSQQLRPGSFLLRYQAFVLSCNFPERPAYGDVSVSSLHARGEAYFYCFTGYQLQGLSTLTCRNASTPFWSGKEPQCVAACGGMVKNATLGRIVSPGFPSNYSNNLTCHWVLEAAEGQRLHVHFEKVALAEDDDRLLIKNGNNIDSAMLYDSYEVEYLPNEGIVSSSRHLFIEFTTDGSGTNTGAAIRYEAFAPGHCYEPFVKYGNFTSSDSSYAVSTVVEFSCDPGYTLEQGSVIIECMDANNPQWNETEPACRAVCSGEITDSAGVVLSPNWPEAYDKGQDCIWGIHVEEDKRIMLDIQVLHMGKNDLLTFYDGDDLTAKILGQYSGSRPRFKLYTSTADVTIQFQSDPATNIYGYNNGFVVHFFEVPRNDTCPELPEITNGWKTTSHPELVHGTVVTYQCYPGFEVVGTEMLMCQWDLTWSGDLPTCERVLSCPDPGTVEHSRRVMSGPRLIVGSTVQYICNKGYSLSGNSLLSCYHHDSTGPKWSEKLPKCIPDTYEPCRNPGTPAFGVQVSEKQFYQAGETLRFSCLSGYKLFGEATIRCVPSHPSKWSHPPPICKAAALEYVNERRLDVARTDFSMEGVNMAIAIFIPVAIIMLIVMSIYFYFSRVQGKSLRLPMSASPQYDQIRGESAFENPIYETANNEVSMSDGSVSVTLACVRGCLSVPVCQSGVNSHLLFVPFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BY16</accession>
    <name>A0A3B4BY16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small integral membrane protein 11A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SMIM11A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BY16"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008848.2">
        <property type="protein sequence ID" value="ENSPNAP00000003369.1"/>
        <property type="gene ID" value="ENSPNAG00000009797.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234765"/>
    <dbReference type="OMA" id="TKMINWK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042125">
        <property type="entry name" value="SMIM11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35975:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35975">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14981">
        <property type="entry name" value="FAM165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="8046" checksum="7A4E606A33001B7F" modified="2018-12-05" version="1">RANMINWRALDNVPLLLYILAAKTLLLCLAFAGAKLYQQKKAEAALKQQMEMKRKLAQQTQELLDNKKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYB2</accession>
    <name>A0A3B4BYB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Adhesion G protein-coupled receptor B3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Brain-specific angiogenesis inhibitor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ADGRB3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Receptor that plays a role in the regulation of synaptogenesis and dendritic spine formation at least partly via interaction with ELMO1 and RAC1 activity. Promotes myoblast fusion through ELMO/DOCK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556420.1">
        <property type="nucleotide sequence ID" value="XM_017700931.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037388535.1">
        <property type="nucleotide sequence ID" value="XM_037532638.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000003551"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008526.2">
        <property type="protein sequence ID" value="ENSPNAP00000003551.1"/>
        <property type="gene ID" value="ENSPNAG00000010187.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429282"/>
    <dbReference type="CTD" id="577"/>
    <dbReference type="GeneTree" id="ENSGT00940000155081"/>
    <dbReference type="OrthoDB" id="5989160at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043083">
        <property type="term" value="C:synaptic cleft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15989">
        <property type="entry name" value="7tmB2_BAI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000002">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000003">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000004">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000012">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000002">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000004">
        <property type="entry name" value="Adhesion G protein-coupled receptor B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043838">
        <property type="entry name" value="AGRB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008077">
        <property type="entry name" value="GPCR_2_brain_angio_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF40">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19188">
        <property type="entry name" value="AGRB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01694">
        <property type="entry name" value="BAIPRECURSOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="878"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="912"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="942"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="981"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1020"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1098"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1125"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="21">
        <location>
            <begin position="501"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="20">
        <location>
            <begin position="693"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="22">
        <location>
            <begin position="876"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1407"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1412"/>
            <end position="1425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1536" mass="171138" checksum="08ACCEB40BD35F99" modified="2018-12-05" version="1">MKAIRNLLIYIFSTYLLVMFGYTGAQDFWCSTLVKGVIYGSYSVNEMFPKNFTNCTWTLENPDPTKYSIYLKLYKRDLSCSQFSLLVYQFDHFSHEKINELLKNNESIVYLCEAPNVYVFLHYDKNFVQLRRVFPYEDNGLVAKKAAEEEEKSVVEFVVLNKASPSQFGCQVLCTWLENCLKGEKGTVESCGVVYSKCTCPQHLGDGESESVLMLNNVVLPLNPQTEGCLTPQLRAAHACNLSAEVKRPSKEEFGMIGEHTVKSQRPRSVHDTKGQQERDESAKFMAQTGDPGAEEWSQWSSCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTAPCPVHGVWEEWSPWSLCSFTCGRGHRTRTRVCTPPQHGGRACDGPETQSKLCNIALCPVDGQWQEWSAWSDCSVTCANGTQQRKRQCSAAAHGGSECRGHWAESRECHNPECTANGQWNPWGPWSGCSKSCDGGWQRRVRVCQGLAVTGQQCEGNGEEVRKCSEQRCPAPYEICPEDYAVSMLWRRTPSGELAFNRCPPNATGTTSRRCSLDHRGVAYWEQPSYARCIANEYRYLQQSVQGHLAKGQRNLAGDGMSQVTKVLLDLTQRRNFYAGDLLSSVEILRNVTDTFKRASYEPSSDDVQNFFQIISNLLEEENREKWEDAQQIYPGSVELMQVIEEFIHIVGLGMKDFHNAYLMTGNLVASIQRLPAVSVMTDINFPMKGRKGMVDWARNSEDKVVVPKGIFLPQTADMDGSPVFILGTVLYKTLGLMLPAPRNNTAVNSKVIAVTVRPEPRVTEAQLEIELAHLANGTMNPFCVLWDSSIMNDSWGTWSSKGCKTVLTDASHTKCLCDRVSTFAILAQQPREITMEYSGVPSVTLIVGCGLSCLALITLAVVYAILWRYIRSERSIILINFCLSIICSNILILVGQTQTHNTGVCTMTTAFLHFFFLASFCWVLTEAWQSYMAVTGKVRTRLIRKRFLCLGWGLPALVVAISMGFTKAKGYGTPQYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHTGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPISADATGTFPNGHAQIMTDFEKDVDIACRSALHKDIGSCRAATITGTLSRISLNDEEEEKGPEGLNYSTLPGNIISKVIIQQPSGLHMPKELSEQCLGDATGDMRRTVYLCTDDTMRQSEQELTGHSQQGPMETDYIVMPRASASAAAASGIGGSGGVPTLLKEDSKMNITMDTLPHERLMHYKVSPEFNISPSGMEHMGMGMGMGMGMEQQFQGGAPEHMQNLPFEPRTAVKNFLAEMEESGGLSRSETGSTTSMSSLERRKSRYQDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNMGSMDKAMPNKNPWESYNPACEYQNYATMNVLESDTKDSLEMTPAEWEKCVNLPLDVQEGDFQTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYS5</accession>
    <name>A0A3B4BYS5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYS5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004847"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006324.2">
        <property type="protein sequence ID" value="ENSPNAP00000004847.2"/>
        <property type="gene ID" value="ENSPNAG00000001605.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159732"/>
    <dbReference type="OMA" id="ESVILWF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388:SF7">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388">
        <property type="entry name" value="T CELL-SPECIFIC ADAPTER PROTEIN TSAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="310"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="132"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004847.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="406" mass="48416" checksum="B210EA9FBB690AC7" modified="2024-11-27" version="2">MMQQILRDMYVEPELLAELNEEQKQILFYKIRQEQVRRWKERENQEDGWERCPKRVQWLHGNDGEVWVWVLGDAPGDKSYEQIIRELIEERAWRQAQLEAQELWRKKEAEIKQKFRDAVAKEKARLVAGKWKEEAEDRKAAKREEERIQEELKKREEEERQKGEEEVRRVEEKRARELYISLEQERKKSEQDNKAWEEQLRRSKEADEEMKKKARWARDEYKRQSIRAIEQGHVAGLTLCCSIMFTMFFCQCSLSLFLSLHLPMSLSLPSPVWVRCPRPSSRESVISWFSEDQKPKRAGYERNSDTIAPWFHGIITREESEKLLKDEAEGSFLVRVSERIWGYTLSYRTATGFKHFLIDASGDYYSFLGVDQNRHATLTDLINFHKVASLREYGLNTRESPSDDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C123</accession>
    <name>A0A3B4C123_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome c oxidase copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">COX17</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Copper metallochaperone essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. Binds two copper ions and delivers them to the metallochaperone SCO1 which transports the copper ions to the Cu(A) site on the cytochrome c oxidase subunit II (MT-CO2/COX2).</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with COA1. Interacts with the chaperone CHCHD4; this is important for correct folding and the formation of disulfide bonds that stabilize the structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C123"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004364"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007152.2">
        <property type="protein sequence ID" value="ENSPNAP00000004364.1"/>
        <property type="gene ID" value="ENSPNAG00000010805.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002329"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000001">
        <property type="entry name" value="cytochrome c oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 14" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="141" mass="15065" checksum="25DFBD67E0CF4B84" modified="2018-12-05" version="1">ADRDSENSSTYHLSTYDPRQSHQAQETGQQGGDREFDSGDVSAVHGRESSTTGLALWVGGTQSVGNPSSCSCKIMSTISAASVEATPAIEGAQEKKPLKPCCACPETKKARDACIIEKGEERCTNLIEAHKECMRALGFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2S8</accession>
    <name>A0A3B4C2S8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69). Interacts with CCR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2S8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005146"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005793.2">
        <property type="protein sequence ID" value="ENSPNAP00000005146.2"/>
        <property type="gene ID" value="ENSPNAG00000037023.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272426"/>
    <dbReference type="OMA" id="ENCCIAF"/>
    <dbReference type="OrthoDB" id="8934837at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000002">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000827">
        <property type="entry name" value="Chemokine_CC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF183">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00472">
        <property type="entry name" value="SMALL_CYTOKINES_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3 9" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043105079" description="C-C motif chemokine" evidence="9">
        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="10">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005146.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="99" mass="10815" checksum="EAC9F23AAE85BED5" modified="2024-11-27" version="2">MRTLSALLVLLLLCSLQLTSSAPLATEQPSCCLYLTPMKIPLAQVVSYTWTSSDCHLKAIVFLTKKGKQICVDPDTVWVSSHVAAVDKLPTMLSLKKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C508</accession>
    <name>A0A3B4C508_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CCAAT/enhancer-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription activator that recognizes two different DNA motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Transcriptional activator that enhances IL6 transcription alone and as heterodimer with CEBPB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the bZIP family. C/EBP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C508"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006155"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004039.2">
        <property type="protein sequence ID" value="ENSPNAP00000006155.1"/>
        <property type="gene ID" value="ENSPNAG00000012227.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163032"/>
    <dbReference type="OMA" id="RRNMEMQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000028">
        <property type="entry name" value="CCAAT/enhancer-binding protein beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031106">
        <property type="entry name" value="C/EBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016468">
        <property type="entry name" value="C/EBP_chordates"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334">
        <property type="entry name" value="CCAAT/ENHANCER BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334:SF3">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005879">
        <property type="entry name" value="CCAAT/enhancer-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0010">Activator</keyword>
    <keyword evidence="6 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="13">
        <location>
            <begin position="199"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="166"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="270" mass="30040" checksum="CDC50E42B6719213" modified="2018-12-05" version="1">MRLFTAASMCDTYSPDSQCVSPPCNMSWALEPANFYESKQIGASADAKAARPDEDGGVVELSNAPAIYDDESAIDFSAYIDSVSSAPSFELCHDELFADLFNSAVKQEKGDFYQLGSAHADFERAEYGSKSAFPPPPIKQEAEWSDGDAPSSLPSQIEACAQTSVSLHTGQPPTPPATPEPAHRRGSRERGKKSVDRLSPEYRQRRERNNIAVRKSRDKAKRRNLEMQQKLVEMSAENERLRKNVEQLTRELSSLRSFFKQLPESIADSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C599</accession>
    <name>A0A3B4C599_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566211.1">
        <property type="nucleotide sequence ID" value="XM_017710722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C599"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006250"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003884.2">
        <property type="protein sequence ID" value="ENSPNAP00000006250.1"/>
        <property type="gene ID" value="ENSPNAG00000012303.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435121"/>
    <dbReference type="CTD" id="553677"/>
    <dbReference type="GeneTree" id="ENSGT00530000063868"/>
    <dbReference type="OMA" id="DYHIEIN"/>
    <dbReference type="OrthoDB" id="10006997at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010591">
        <property type="term" value="P:regulation of lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11284">
        <property type="entry name" value="ADF_Twf-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11285">
        <property type="entry name" value="ADF_Twf-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000007">
        <property type="entry name" value="Twinfilin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000012">
        <property type="entry name" value="Twinfilin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028458">
        <property type="entry name" value="Twinfilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759">
        <property type="entry name" value="TWINFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13759:SF9">
        <property type="entry name" value="TWINFILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ADF-H" evidence="10">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="ADF-H" evidence="10">
        <location>
            <begin position="191"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="332"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="363" mass="41677" checksum="EB7ED52AAB2F96D7" modified="2018-12-05" version="1">MSHQTGIHATADLREFLVKARRGAVRVMKIVIRSEQLVLGAYREPSQSWDRDYDPCILPLLDSMEPCYILFRLDTQNQQGYEWLFISWSPDQCPVRLKMVYAATRATLKKEFGGSHIKDELFGTTQEDVCFQGYLRHLSSCSSPAPLTSAEQELHQIKATEEERRRVAAPSGRGKGKTEISVESKHSTLQGLAFPLQEEAKRALEQLKQRRINYIQLRLDTERETIELVHTNPTDIRELPARIPADVPRYHFFIYKHSHQGQSMEAVVFIYSMPGYSCSIKERMLYSSCKNRLLEEVERDYHIEITKKMEIDSGEGLTEEYLYEEVHPKPQALKQAFTKPKGPSGKRGNKRLIKGAGENGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7C4</accession>
    <name>A0A3B4C7C4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lens intrinsic membrane protein 2.3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PMP-22/EMP/MP20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566545.1">
        <property type="nucleotide sequence ID" value="XM_017711056.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C7C4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006796"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002927.2">
        <property type="protein sequence ID" value="ENSPNAP00000006796.1"/>
        <property type="gene ID" value="ENSPNAG00000004756.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435296"/>
    <dbReference type="CTD" id="436860"/>
    <dbReference type="GeneTree" id="ENSGT01050000244814"/>
    <dbReference type="OMA" id="CMPGKCM"/>
    <dbReference type="OrthoDB" id="6137544at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000011">
        <property type="entry name" value="lens fiber membrane intrinsic protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003935">
        <property type="entry name" value="LMIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004032">
        <property type="entry name" value="PMP22_EMP_MP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF25">
        <property type="entry name" value="LENS INTRINSIC MEMBRANE PROTEIN 2.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01457">
        <property type="entry name" value="LENSMEMPROT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01221">
        <property type="entry name" value="PMP22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01222">
        <property type="entry name" value="PMP22_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19544" checksum="2E21559F604E00E0" modified="2018-12-05" version="1">MYSFMGGGLFCAGVGNILLVVSTATDYWMQYRQSSGFMHQGLWRYCMPGKCMTHTDSIAYWDATRAFMILAILACFFGLIIGVMAFINYSSFGGFDKTFAAGILYFISCFFVLLAMAVYTGVTVNYYGKRYGSWRFSWSYIMGWVSVVLTFFSGIFYMCAYRMHGPRGPMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAJ7</accession>
    <name>A0A3B4CAJ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CCR10</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556196.1">
        <property type="nucleotide sequence ID" value="XM_017700707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAJ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008633"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014762.2">
        <property type="protein sequence ID" value="ENSPNAP00000008633.1"/>
        <property type="gene ID" value="ENSPNAG00000014227.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429159"/>
    <dbReference type="CTD" id="2826"/>
    <dbReference type="GeneTree" id="ENSGT01030000234667"/>
    <dbReference type="OMA" id="CYAINTY"/>
    <dbReference type="OrthoDB" id="8957211at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF735">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="75"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="364"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="431" mass="47634" checksum="627F47DBC9991DA7" modified="2018-12-05" version="1">MADYNSSAGSSENFTATDVVLTDFPTATDDYGDYTFVSTNTALLNPCEGNALQETYSKVVQVTVFLIVLVLGVIGNVLVIATFARYRCLRVRCMTDVFLFYLALSDLLLLLTLPLQTVETIRGDWVFGDVLCKLNHAMHAINTYGGLLLLACISVDRYLVVVRARAAHRLHPSMLCHSKLSVIAVALISVILSLPELLFSSVVKVKLDDMETCGMNIWVKDSRRVKKWARVAKITVFCIPCVVMLVCYMAIGYKLVQGGRKCWRRQRTLLLMVALVVLFLLFQLPYNIVLTLRETTDLNSCELWGSAHLSEDITRSLAYVRCCLNPVLYALVGVRFRNDVMRLLQDCGCVCACLKHMKPLPTGCSSMTPSSPPPTTLSPLPSASGPPKNLLACSRPSQPQADAEESRAPDTLLSSAQQSHKDPAYVSWGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CB65</accession>
    <name>A0A3B4CB65_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CB65"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001450.2">
        <property type="protein sequence ID" value="ENSPNAP00000007674.2"/>
        <property type="gene ID" value="ENSPNAG00000002741.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177011"/>
    <dbReference type="OMA" id="ICELPRH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007674.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13297" checksum="8C20BA3A22BDC460" modified="2025-10-08" version="3">MWSVPFLLGSPSAPITFPSARMLPVLRMRSDPARSTKWNLEERICRLPLVAREPPRRCSMRFIWVAPVCRAVFPESKRLKTSSSPSTSHSLTPHRTMGKHGKTLSYEHHSVPFLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEM3</accession>
    <name>A0A3B4CEM3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Spastin</fullName>
            <ecNumber evidence="10">5.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 16" type="primary">SPAST</name>
        <name evidence="10" type="synonym">SPG4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.</text>
            <dbReference type="EC" id="5.6.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Cytoskeleton</location>
            <location evidence="10">Microtubule organizing center</location>
            <location evidence="10">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
            <location evidence="10">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <text evidence="10">Forms an intramembrane hairpin-like structure in the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the AAA ATPase family. Spastin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.1" evidence="10"/>
    <dbReference type="RefSeq" id="XP_017571990.1">
        <property type="nucleotide sequence ID" value="XM_017716501.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEM3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008929"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015145.2">
        <property type="protein sequence ID" value="ENSPNAP00000008929.1"/>
        <property type="gene ID" value="ENSPNAG00000014543.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438595"/>
    <dbReference type="CTD" id="6683"/>
    <dbReference type="GeneTree" id="ENSGT00940000156258"/>
    <dbReference type="OMA" id="KSREPML"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021955">
        <property type="term" value="P:central nervous system neuron axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032506">
        <property type="term" value="P:cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045773">
        <property type="term" value="P:positive regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031117">
        <property type="term" value="P:positive regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02679">
        <property type="entry name" value="MIT_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19524">
        <property type="entry name" value="RecA-like_spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000036">
        <property type="entry name" value="Spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000006">
        <property type="entry name" value="Spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03021">
        <property type="entry name" value="Spastin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017179">
        <property type="entry name" value="Spastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF86">
        <property type="entry name" value="SPASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="10">
        <location>
            <begin position="72"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="MIT" evidence="15">
        <location>
            <begin position="103"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="346"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="189"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="354"/>
            <end position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="589" mass="65196" checksum="3255CE5CB54AF16D" modified="2018-12-05" version="1">MNSAPGGRARSLPGSAPPSCRDRASPVRGSSLPARRLFSWIHPLFSWIYPLFSWIYPLFSWIYPLFSWIYPLFSWTCRLFRRSMAARPTELGAAEPDAAGERIRNLHKQAFEYISVALRIDEDEKGHKDQAVEWYRKGITELEKGIAIQVTGTGEKSDRARRLQAKMTTNLTMAKDRLELLAKLRPDSHRSVYTDGSDQALSNGSLRRVSGAVPKKRDSNTSQAKVKQPTRPPSSTPQRTHSFTSAPTGATGRTGQHKAAAGRGGGRGNVKAGGAPSSAPQPKRDMKNFKNVDSKLANLILNEIVDSGSAVRFEDVAGQDLAKQALQEIVILPALRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAMESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIQFDGVQSGGDDRVLVMGATNRPQELDEAVLRRFPKRVYVSLPSEETRLKLLKNLLAKHGNPLTQKELSQLARMTDGYSGSDLTSLAKDAALGPIRELRPEQVRNLAANEMRNIRFSDFVDSLKKIKKSVSPQTLDQYVRWNREYGDTTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFY1</accession>
    <name>A0A3B4CFY1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TBC1D22B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571950.1">
        <property type="nucleotide sequence ID" value="XM_017716461.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFY1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009781"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016234.2">
        <property type="protein sequence ID" value="ENSPNAP00000009781.1"/>
        <property type="gene ID" value="ENSPNAG00000015255.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438566"/>
    <dbReference type="CTD" id="55633"/>
    <dbReference type="GeneTree" id="ENSGT00940000157472"/>
    <dbReference type="OMA" id="SCYNIFN"/>
    <dbReference type="OrthoDB" id="26371at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071889">
        <property type="term" value="F:14-3-3 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000009">
        <property type="entry name" value="TBC1 domain family member 22A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000001">
        <property type="entry name" value="TBC1 domain family member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000004">
        <property type="entry name" value="TBC1 domain family, member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF462">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="204"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="499" mass="58304" checksum="9833B5901CC5BD79" modified="2018-12-05" version="1">MATENKLHFWRRNAKVPGRFLKDTPKTNIANIKSKKPSTFHEFARSTNDAWDIDDEEDNDFFTPSHPSSIPAQSHSKPQTPRLKVSKHFTDTEPQPQPLNRLEPVSTEICPDQLRIDGEPTNGRVVKSSSDAQLNSNTARGTLQKQQSLPVRPVIPLVARISDQNASGAPPMTVREKTRLDKFRQLLASPNTDLEDLRKHSWSGIPREVRPITWRLLSGYLPANMERREQVLRRKREEYFGFIEQYYHSRTDEHHRDTYRQIHIDIPRTNPLIPLFQQPLVQEVFERILFIWAIRHPASGYVQGINDLVTPFFVVFLSEFVEEDVENFEMAALPLETQRNIEADSFWCMSKLLDGIQDNYTFAQPGIQTKVKALEELVSRIDEDVHLHFKKCEVEYLQFAFRWMNNLLMRELPLRCTIRLWDTYQAESEGFSHFHLYVCAAFLIKWRKEILSMSDFQGLLMLLQNLPTIHWGNEEVGLLLAEAYRLKYMFADAPSHYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CG80</accession>
    <name>A0A3B4CG80_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">6-phosphofructo-2-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CG80"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009459"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035322.2">
        <property type="protein sequence ID" value="ENSPNAP00000009459.2"/>
        <property type="gene ID" value="ENSPNAG00000014977.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182835"/>
    <dbReference type="OMA" id="CIESDRY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
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        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF14">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="12">
        <location>
            <begin position="54"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="9">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="270"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009459.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="483" mass="56290" checksum="A072CF282658EF79" modified="2024-11-27" version="2">MNKEGIHFQFAFHFGSFHMSVWRADSPSYLHFFTLVSPFCLFIFFISCFLSVCMTNCPTLIVTVGLPARGKTYISKKLTRYLNWIGVPTKEFNVGQYRRECLKIYKNFEFFRPDNEEGLKIRKQCALTALNDVRQYLSVEGGQVAVFDATNTTRERRSMILRFSEQNGYKVLTSSSGYYCVCKQQVKLGSPDYIDCNTEEAIQDFMKRIKCYESSYQPLDEILDRELSYIKVMDVGRQYLVNRVLDHIQSRIVYYLMNIHITPRSIYLCRHGESELNIRGRIGGDSGLSTRGKEFALCLGKFIQEQNIKDLKVWTSQMKRTIQTAEALGVQYEQWKALNEIDAGVCEEMMYEEIQEHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENILVICHQAVMRCLLAYFLDKSADELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHRDKPENVDICRLAEEALLTVPAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJ97</accession>
    <name>A0A3B4CJ97_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539741.1">
        <property type="nucleotide sequence ID" value="XM_017684252.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJ97"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010584"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017304.2">
        <property type="protein sequence ID" value="ENSPNAP00000010584.1"/>
        <property type="gene ID" value="ENSPNAG00000016164.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412279"/>
    <dbReference type="CTD" id="335655"/>
    <dbReference type="GeneTree" id="ENSGT01100000263525"/>
    <dbReference type="OMA" id="SWLEIAW"/>
    <dbReference type="OrthoDB" id="9933679at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="69" mass="7809" checksum="1141A327447747C2" modified="2018-12-05" version="1">MARDMSDKDILKMEIEQLQKEVKNTREPVSKTAKELCEFVEAQSAEDPLIKGVPEDKNPFKEKGGCIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPA8</accession>
    <name>A0A3B4CPA8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPA8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019955.2">
        <property type="protein sequence ID" value="ENSPNAP00000012631.2"/>
        <property type="gene ID" value="ENSPNAG00000036701.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="49"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012631.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="18713" checksum="8AFDCABCF1CDEC67" modified="2024-11-27" version="2">MELEFFPLFPFIILGHFFWSIHHKMNTQCKGQDYLKKKLKNGKNGDTRGESVLLPCPSPFEYGDTVSVKWEKDGESGVCMYRMSENKTSGHVCSPRFKVNADPIGLIITDVTGSDAGNYNCSMIRMIPPPAVDDFSILILQVNGDYTLLYCMHIKRLSPFIFSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQI5</accession>
    <name>A0A3B4CQI5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">26S proteasome regulatory subunit p27</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with PSMC3. Part of a transient complex (modulator) containing PSMD9, PSMC6 and PSMC3 formed during the assembly of the 26S proteasome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571824.1">
        <property type="nucleotide sequence ID" value="XM_017716335.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQI5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014332"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037276.2">
        <property type="protein sequence ID" value="ENSPNAP00000014332.1"/>
        <property type="gene ID" value="ENSPNAG00000020236.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438493"/>
    <dbReference type="CTD" id="5715"/>
    <dbReference type="GeneTree" id="ENSGT00390000004147"/>
    <dbReference type="OMA" id="DWGGRGM"/>
    <dbReference type="OrthoDB" id="72325at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000107">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040815">
        <property type="entry name" value="Nas2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18265">
        <property type="entry name" value="Nas2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="83"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="213" mass="23825" checksum="07750B49F0515ED7" modified="2018-12-05" version="1">MKMTEENNNNNPTITVEDVQRLIKKKDEMEEQIKAYYDVLEDQGDVGMDAPLVDVEGYPRADVDLFQIRTARHSISCLQNDHKAIMVEIEEALHKLHARERAKREQDQAEAQVERMEQEASLPAPFARVDAVTQGSPAYQAGLHIGDEIIAFGSVNTSNFQNLQNIASVVQHSEGKSVSIALVRNGQKVNLSLTPQRWSGRGLLGCNIVPLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQY5</accession>
    <name>A0A3B4CQY5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">C2orf68</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0561 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQY5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012954"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020405.2">
        <property type="protein sequence ID" value="ENSPNAP00000012954.2"/>
        <property type="gene ID" value="ENSPNAG00000018774.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001901"/>
    <dbReference type="OMA" id="FCLEYEG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018888">
        <property type="entry name" value="UPF0561"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34256">
        <property type="entry name" value="UPF0561 PROTEIN C2ORF68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34256:SF1">
        <property type="entry name" value="UPF0561 PROTEIN C2ORF68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10573">
        <property type="entry name" value="UPF0561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012954.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="164" mass="19386" checksum="D74B6C33FF094AAA" modified="2024-11-27" version="2">MEIWQNAEGETLKYKPGGRLDMTHGFLHHIRRNQIARDDYDKEVKQAKERQKRRHTATPRRPRRPDREVYHPRHRNGSEPGTGVEIEEWNESSSSTEQESTGSKLFWLDYQADNGHVTSFIVYKEDEPESVVERVEEKNVLDSAMRTVLLARIGQEMDKRRDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRQ7</accession>
    <name>A0A3B4CRQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">EBF2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the COE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037387521.1">
        <property type="nucleotide sequence ID" value="XM_037531624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CRQ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013436"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036940.2">
        <property type="protein sequence ID" value="ENSPNAP00000013436.2"/>
        <property type="gene ID" value="ENSPNAG00000019339.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438472"/>
    <dbReference type="GeneTree" id="ENSGT00950000182859"/>
    <dbReference type="OMA" id="QGYMRNS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11606">
        <property type="entry name" value="COE_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01175">
        <property type="entry name" value="IPT_COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4280:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000021">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3180:FF:000002">
        <property type="entry name" value="transcription factor COE2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3180">
        <property type="entry name" value="Transcription factor COE1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032200">
        <property type="entry name" value="COE_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038173">
        <property type="entry name" value="COE_DBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032201">
        <property type="entry name" value="COE_HLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038006">
        <property type="entry name" value="COE_IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003523">
        <property type="entry name" value="Transcription_factor_COE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018350">
        <property type="entry name" value="Transcription_factor_COE_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10747">
        <property type="entry name" value="TRANSCRIPTION FACTOR COE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16422">
        <property type="entry name" value="COE1_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16423">
        <property type="entry name" value="COE1_HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01345">
        <property type="entry name" value="COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="IPT/TIG" evidence="13">
        <location>
            <begin position="261"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="444"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="502"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="451"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="582" mass="63279" checksum="F17AB0CDC1499475" modified="2024-11-27" version="2">MFGAQEHLAGEVASVKERSFEEEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFVLALYDRHGQPVEIERTAFVDFVEHEKEQTGEKTNNGTHYKLQLLYSNGVRTEQDLYARLIDSVTKQPISYEGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRFQVVLSSTVSVDGHVLAVSDNMFVHNNSKHGRRARRLEPGETLENPMEYATPCIKAISPSEGWTTGGAMVIIIGENFFDGLQVVFGSMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPEKLAKEMLLKRAADLVEAVYGNTHSNQDMLLKRAADIAEALYSVPRPHSQLQALPSSPVHGSVMGLSSYPSQLGVSIGEPGQTSGQGYMRNSSLSPRGYPSASTPQQSSYGSGGGMSGGYGAVPMSSLGVPGSPGFSSASPNSSPYVMPSSPTIPGSSSTSSLLPFSSFPSTAKQKSAFAPVLRPQGSPSPACPTTGGNGFRAMAGLVVPPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTI9</accession>
    <name>A0A3B4CTI9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">IL6ST</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552780.1">
        <property type="nucleotide sequence ID" value="XM_017697291.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTI9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015402"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023591.2">
        <property type="protein sequence ID" value="ENSPNAP00000015402.1"/>
        <property type="gene ID" value="ENSPNAG00000021433.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427275"/>
    <dbReference type="CTD" id="3572"/>
    <dbReference type="GeneTree" id="ENSGT00940000155603"/>
    <dbReference type="OMA" id="RYILEWC"/>
    <dbReference type="OrthoDB" id="9934532at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048680">
        <property type="term" value="P:positive regulation of axon regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003529">
        <property type="entry name" value="Hematopoietin_rcpt_Gp130_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010457">
        <property type="entry name" value="IgC2-like_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06328">
        <property type="entry name" value="Lep_receptor_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01353">
        <property type="entry name" value="HEMATOPO_REC_L_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017316424" description="Fibronectin type-III domain-containing protein" evidence="13">
        <location>
            <begin position="19"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="623"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="232"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="523"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="723"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="795"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="862" mass="96938" checksum="CD5A186E4B597755" modified="2018-12-05" version="1">MSTSLHLICVMFFGCAFGLIQQCAKILPDSPYQISVEVGTEFTAMCILTHSQYTSEDIEWIMGNVTLPRESYKKINESAVSVTMKISTSMNNPLKCSASARTYSLNTYSSFKNPCTYGIYLQKGYPPLQPKNLTCVALQVGKLISPDLTCSWDQETRDTLIPTTYTLFARILGGQQYDSFSKDPHTRSLTVNLVTFPNHMMLEVWVEVNNTLGQVTLKEGEKESEEFVKPNPPANVKVIPEDKFPSSLMVNWTHPIHSTTFKLKYNIRYRKAGSGIWTEVPQDFTKAYTESFRLQFLEPYTDYEVQMRCINQKGLGYWSDWSSSVTVRTPEAIPGSRPDLWRVYGDDDSVVKLIWKDPVKSYGKILSYNLTIGEGNSPERLVVYSKEYQFHLKRESVSVKITASNSVGVSPPSALVISRHEASRGVDQVIYSVLDGQLQVDWLPPTQIGKNVSEYLLEWVSVPDKQRDWQRVSGELNNTILKGNFQPFIRYNISIYPIYKEKKHYNRPGKGFTVEAYLQQGPPLKGPSVDVSNTQKNSAFLKWYEIPIDSQRGFLTNYTIFYKTGDTDQSVVVEPHVSSYKLTGLASKSQYVVHIMASNVEGSVNGSDSSFYTKQYDDGEIEVIVVLVCLGFLFFIAFIMMLSLKNREVIKKHLWPQVPDPYHSTIATWSPDCPIKTDTPKEATLPDVSVVEVDVFDGKSLCEEDKTMLPLKKDKYFSEEHSSGIGGSSCMSSPRQSVSDSDEGDSGQTTASTVQYSSVVASGYKGQTPSLQAPVFARSESTQPLLDCEEHQEHLLDGGSQQRNSYFKRGRGLEQLQPQDTEEPGCFSPTEEEDTPTLTEDPPGPAPGYMPQQSGYRPQRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CUT0</accession>
    <name>A0A3B4CUT0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CUT0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014279"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022141.2">
        <property type="protein sequence ID" value="ENSPNAP00000014279.2"/>
        <property type="gene ID" value="ENSPNAG00000020224.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160790"/>
    <dbReference type="OMA" id="MDIPRPE"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0346">Stress response</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043433664" description="mitogen-activated protein kinase" evidence="19">
        <location>
            <begin position="17"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="265"/>
            <end position="543"/>
        </location>
    </feature>
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        <location>
            <begin position="545"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
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        <location>
            <position position="295"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50011"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014279.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="581" mass="64700" checksum="A677CE5DB879E99C" modified="2024-11-27" version="2">MALGLSLSLFLSQSLSLSLSLSPSLSRSLSVSLSLSVSLFFCLSLSFSLCLSLSISVSLSQSLSLSFSVSLFFSLSLSFFSVSLSFSLSLSLSLFLCVSLSFSLSLSLSLSLSLFLSLSLSFSVSLFFSVSLPLSISVSLSLSLSLCLSLSLFLCLSLSFSLSLSFSLSLSLSLSLSLSLSLSLSLSSPTDGQTAPPILVNCEEEGRLRGWGANRLGGSDISLEHPEKWTLPPVSLPPLVERMTARPGFYRQELNKTVWEVPERYQNLTPVGSGAYGSVCSAYDVCLRQKVAVKKLSRPFQSLIHSRRSYRELRLLKHMKHENVIGLLDVFTPAASLEEFNEVYLVTNLMGADLNNIVKFQRLSDEHVQFLIYQLLRGLKVIQKDLKPSNVAVNEDCELRILDFGLARQTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMGELLKGKVLFPGNDYIDQLKRIMEVVGTPTADLLKKISSEHAQKYIQSLPYMPQQDLGKIFRGANPLAVDLLKRMLVLDCDRRISASDALCHPYFSQYHDPDDEPEAPLYDQTPESKDRTLEEWKGQRGRSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CUX6</accession>
    <name>A0A3B4CUX6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000015200"/>
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        <property type="gene ID" value="ENSPNAG00000021216.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0391">Immunity</keyword>
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    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="22"/>
            <end position="115"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015200.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="120" mass="13633" checksum="D00B360AA6395879" modified="2024-11-27" version="2">MNRISLLISLCHTEGTDVFRKEGEPVTLKCSYDSTSSLVLLYWYRQHPNRALQFILYKAAPSSSAEHIPDSRFTSTTTSSSTELRVRELRLADTALYYCALQVGAQWQNNTINITCRPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CXI9</accession>
    <name>A0A3B4CXI9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CXI9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015254"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023438.2">
        <property type="protein sequence ID" value="ENSPNAP00000015254.2"/>
        <property type="gene ID" value="ENSPNAG00000021314.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164248"/>
    <dbReference type="OMA" id="YIIRAMG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070052">
        <property type="term" value="F:collagen V binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010811">
        <property type="term" value="P:positive regulation of cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000772">
        <property type="entry name" value="Extracellular matrix protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001007">
        <property type="entry name" value="Si:dkey-32e6.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043184">
        <property type="entry name" value="ECM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46544">
        <property type="entry name" value="EXTRACELLULAR MATRIX PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46544:SF2">
        <property type="entry name" value="EXTRACELLULAR MATRIX PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VWFC" evidence="4">
        <location>
            <begin position="43"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015254.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="614" mass="68882" checksum="C62D46738E875B57" modified="2024-11-27" version="2">MILSSCPRSVVHGCLALIAFSKGASFLGGRITVAHYNELRSQCLVNGIFLFEGAVWSPDSCSVCQCRSGAVRCHAVPCLDAGTQFTALLLESPYPCSSLSIYPPPPPIKRLEIVKPQTQPQWWDRKLVLGPSVVERRRFDHDDHDDDDYMEDDSDSNDDDDDDRTFLISTFPAQKPALPPTLRPFALPTKQSVVLPAGRPLPVPGKHSAGPRGPGRPFIAPYVYMESLPAGCLLYESLIACGSTGLTHLPLIKDTGLRILYLSDNKISKIPTRAFAGLPNLEWLDLSKNKLDDFSLGQDVFKNLTKLRRLNLDGNNLTKVPPLPPSLLELKINDNKLSGLTPHSFRGVSQLLTLELEDNEFHDGNVSPLAFRNLGKLIYLRLDDNDFRAIPSGLPTSLQELRLSDNQIEVVHAGLLNKTVNLRVLNLSHNKIREDRIAPRAWIHLRKLESLDLSYNKLVHVPSFLPVGLRQLMLHHNQIERIPGYVFAHLKPGLDSLDLSYNRLREDGVSEVSFMGLYHSLTELLLDHNQLRAIPRGIIQLRALQLLHLNHNYISSVPMNSLCDSTAREDSPLLSVHLQNNLIERRLIPPTALSCIRTYHSVLLRPQRHEYIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYG1</accession>
    <name>A0A3B4CYG1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26 27">Fatty acid 2-hydroxylase</fullName>
            <ecNumber evidence="27">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">FA2H</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Catalyzes stereospecific hydroxylation of free fatty acids at the C-2 position to produce (R)-2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis. Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath. Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier. Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis. Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="24">Catalyzes the hydroxylation of free fatty acids at the C-2 position to produce 2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis. FA2H is stereospecific for the production of (R)-2-hydroxy fatty acids. Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath. Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier. Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis. Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-saturated fatty acid + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = a (R)-2-hydroxy fatty acid + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:38855"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:76177"/>
            <dbReference type="ChEBI" id="CHEBI:83955"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:38856"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>docosanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = 2-hydroxydocosanoate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:39819"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:23858"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:76722"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:39820"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hexadecanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (R)-2-hydroxyhexadecanoate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:38551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:75927"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:38552"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>octadecanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (R)-2-hydroxyoctadecanoate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:39815"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57562"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:39816"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>tetracosanoate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (R)-2-hydroxytetracosanoate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:38559"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:75935"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:38560"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="29">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="27 28">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="27 28">Binds 2 Zn(2+) ions per subunit that likely form a catalytic dimetal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="25">Sphingolipid metabolism; galactosylceramide biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the sterol desaturase family. SCS7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="27"/>
    <dbReference type="RefSeq" id="XP_037396672.1">
        <property type="nucleotide sequence ID" value="XM_037540775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYG1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025828.2">
        <property type="protein sequence ID" value="ENSPNAP00000017132.2"/>
        <property type="gene ID" value="ENSPNAG00000023385.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413383"/>
    <dbReference type="GeneTree" id="ENSGT00390000002142"/>
    <dbReference type="OMA" id="WTIIEYV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000011">
        <property type="entry name" value="Fatty acid 2-hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005149">
        <property type="entry name" value="IPC-B_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 27" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18 27" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10 27" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 29" id="KW-0349">Heme</keyword>
    <keyword evidence="15 27 29" id="KW-0408">Iron</keyword>
    <keyword evidence="5 27" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16 27" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 27 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 27 28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0492">Microsome</keyword>
    <keyword evidence="14 27" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 31" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 28" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="32">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="29">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="29">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005747"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050698"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051519"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051625"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052862"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00053017"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00054885"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060649"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRNR" id="PIRNR005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017132.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="369" mass="43161" checksum="917EDFB0CF52011B" modified="2024-11-27" version="2">MSPSVSPRFFSAKEVAKHCSKESCWVLKGTRVYDVTEFIRMHPGGEALILRRCGKDISRELEGPPHRHSENARRWMEQYYIGELDRNSTEHYTETLRQRGSRQEPNETEEDSGISKCSKVNDETDLVDWRKPLAWQVGHLGEKYDAWVHQPVDRPIRLFESDFFEANTKTAWYMVPAVWMPLVIYLSWYCYSLLAQGSTRLFIASDYSILIHKYSFPFIFLVGVFMWSFVEYCIHRFLFHMRPPAHNYYLITLHFLLHGQHHKSPFDGSRLVFPPGIASIVIGAFYLILKQLLPEGLGLSLFVGGLCGYVIYDMIHYYLHYGSPRKGSYLYGLKAYHVKHHFEHQRAGFGITTTFWDYPFNTVIPKQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYZ7</accession>
    <name>A0A3B4CYZ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytosolic fatty-acid binding proteins domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537061.1">
        <property type="nucleotide sequence ID" value="XM_017681572.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYZ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017312"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038473.2">
        <property type="protein sequence ID" value="ENSPNAP00000017312.1"/>
        <property type="gene ID" value="ENSPNAG00000023543.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410483"/>
    <dbReference type="CTD" id="568032"/>
    <dbReference type="GeneTree" id="ENSGT00940000160165"/>
    <dbReference type="OMA" id="EFEECTK"/>
    <dbReference type="OrthoDB" id="354351at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="3">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="135" mass="15793" checksum="3141340F29512610" modified="2018-12-05" version="1">MPADYNGRWEMVSNENFEELMKSLDIDFATRKIAAHLSQTKVIVQNGDKFETKTLSTFRNYEVNFTVGEEFEEHTKGLDNRVVKTLVQWDGDKLVCVQTGEKKNRGWKHWIEGDLLHLEVTCEDNVCHQVFKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ90</accession>
    <name>A0A3B4CZ90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CDH18</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ90"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016633"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025163.2">
        <property type="protein sequence ID" value="ENSPNAP00000016633.1"/>
        <property type="gene ID" value="ENSPNAG00000022800.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432246"/>
    <dbReference type="CTD" id="767768"/>
    <dbReference type="GeneTree" id="ENSGT00940000157512"/>
    <dbReference type="OMA" id="EAVAWHN"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000265">
        <property type="entry name" value="Cadherin 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000017">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
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        <property type="entry name" value="Cadherin-like_sf"/>
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        <property type="entry name" value="Cadherin_CS"/>
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        <property type="entry name" value="Cadherin_Y-type_LIR"/>
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        <property type="entry name" value="Catenin-bd_sf"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="8 13" id="KW-0130">Cell adhesion</keyword>
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            <begin position="1"/>
            <end position="27"/>
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            <begin position="28"/>
            <end position="819"/>
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            <end position="182"/>
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            <begin position="706"/>
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            <begin position="768"/>
            <end position="802"/>
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            <begin position="44"/>
            <end position="53"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
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    <sequence length="819" mass="89077" checksum="7821E65C187842AD" modified="2018-12-05" version="1">MRAAGGGAACLSSLLLLCLFALRRGLGTPSPAGPILSPRIHNQTKTAEGSVRTTRTHNLTKHLPEGEKDPHHRPKRGWVWNQFFVLEEHIGPEAQYVGKLHSNSDKGDGSVRYLLSGEGAGTIFTINEVTGDIHATKSLDRETKNHYVLHARAIDRFTNRAVEPESEFIIKVQDVNDNAPTFPDGPFAASVPEMSDIGTSVFQITATDADDPTYGNSARIVYSILQGQPYFTVDPKTGIIRTALANMDRETREMYSIIIQAKDMAGSVGGLSGSTTVNITLTDVNDNPPRFPQKNYQLYVPESAQVGKAVGKIKANDEDLGINAEMAYRITNEEGAAMFSIYADSDKREGVISLKKPLDYEKKKAYSLSIEGVNTHLDPQFSHLGPFKDTTTLKIIVGDVDEAPVFTMDYYIMDVYENAPAGADVGMVTAQDPDNARGKVRYSIDSSVNGESLFSIDANSGLIKTTASLDREAVAWHNITVKASEVDNPKLVSYVPVTVQVLDINDNTPYIPTDQDIIVCESSKPGQVIQTIRATDNDNFANGRFSFALPTDLPTNPNFTLKDNEDSSASIIARRWGFSQSEQELHQLAVVVWDGGEPQLSSTSTLTLRVCPCRRGLRVPVCQPQAFFSSAGLSTGALIAILLCILILLAIVVLFVTLRSKKIKKEALIISEEDVRENVVTYDDEGGGEEDTEAFDIVALRNPSAAGEVRRRRGEPQTEVWGGAVGGDSLSPSLTLPSQDIHDVIQLKVMEADQDTSGPPYDSLQTYAYEGQGSPSGSISSLDLPGPPSEPEPPEPDDWGPEVNTLARLFQERGGQCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D144</accession>
    <name>A0A3B4D144_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Synaptotagmin-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Exophilin-7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017724057.2"/>
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    <dbReference type="STRING" id="42514.ENSPNAP00000016574"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025120.2">
        <property type="protein sequence ID" value="ENSPNAP00000016574.2"/>
        <property type="gene ID" value="ENSPNAG00000022777.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443410"/>
    <dbReference type="GeneTree" id="ENSGT00940000155843"/>
    <dbReference type="OrthoDB" id="195679at2759"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08393">
        <property type="entry name" value="C2A_SLP-1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04020">
        <property type="entry name" value="C2B_SLP_1-2-3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Synaptotagmin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="Rab_BD"/>
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        <property type="entry name" value="SYTL1-5_C2B"/>
    </dbReference>
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        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SYNAPTOTAGMIN-LIKE PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RabBD" evidence="12">
        <location>
            <begin position="10"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="710"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="844"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="96"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="302"/>
            <end position="401"/>
        </location>
    </feature>
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            <begin position="545"/>
            <end position="573"/>
        </location>
    </feature>
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        <location>
            <begin position="592"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="330"/>
            <end position="346"/>
        </location>
    </feature>
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            <begin position="545"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="610"/>
            <end position="621"/>
        </location>
    </feature>
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            <begin position="634"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="653"/>
            <end position="665"/>
        </location>
    </feature>
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        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004184"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022483"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00072163"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00075525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016574.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1008" mass="111119" checksum="3BCCFF811B47D135" modified="2024-11-27" version="2">MNSFKDATLFIDLSFLSTEEEAVIRQVLLRDEDLKRLEIGRVRRLRNSVPDHKQLKVLSGEWFEELRSKRYGRQPAISAVVRSSMRWKKSAGHKPLPFLNAVPEEKDGEKEEKKTHPEVSADPRSIHPALSVEVSREYKDEDITLEDETQASQTPVKNVPPEEPSTAKLNNPVTGTTVRGSLSAGKPKEVSQEKTILIQSSKLEPGLQPKSTGAVAVSTTASATTANAVLNAATLGAPTSGKTNAPTSAATRVTSANAASIATASAEPRRKVSLPPALQKDASFEENWVSLEANTVESTAVEIKPKATDGNMPVETLAITSFSETRDASSQDQSRQPGSSADSISTGPKVEAGEIISSRKVTKIEVKQKQARTVTEPVMNKDGSGLGEEVAQSQSLREDPKEAHELLIGSLQGQESSPKLFTLSSVEMMKKQAPASNTAPQMQEEIIISDFKRTSPTRMDELDVNVDESLEYKNTGPAVDLNVGKDTRLDQNRSAETSRDRLAGAEVVIKNAVPERKGGFDVELRDSERADKKTEELELVTKIDKTDELPNADEGRAFSDMADESNATKDECRSENDILASVYARALRAKSPLSKNTSIQDPKQEKAEDVIPSIVIVPSESSEPKDMESLESGTSTPVAPAETPATPSFVADTWEDEGVDSEDDSSSVSSYGSDLSGRRGFSASTLSVTERTGSLLSVYSDAGDFGNVVVQGSVEFALMYSPLGELVIMVEQCQDLAIANPRKQRTDPYVKTYLYPDKRSKRKTSIKKRTLNPVFVESLRYKVKREELPGKTLNLSVWHNDSLGRNVFLGQVELSLKNWDWGHQALTWYNLQPKNADNQESPEYCGLLSVSLKYIPPEPRGTSKLGTGEIHVWLREARQLRRLKAQAVDSFVKCYMLPDTSKKSRQKTRVMKKSLNPVYNHTMVYDGFRPDEVREACCELTVWDNNKLSNQFLGGLRLSLGTGQSYGKKVDWMDSANEEVEVWQKMMAKPNSWVEADLPLRASMTPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D1I3</accession>
    <name>A0A3B4D1I3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PDP2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547086.1">
        <property type="nucleotide sequence ID" value="XM_017691597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D1I3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018192"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027277.2">
        <property type="protein sequence ID" value="ENSPNAP00000018192.1"/>
        <property type="gene ID" value="ENSPNAG00000024528.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423909"/>
    <dbReference type="CTD" id="57546"/>
    <dbReference type="GeneTree" id="ENSGT00940000160687"/>
    <dbReference type="OMA" id="DHNAWNP"/>
    <dbReference type="OrthoDB" id="420076at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF343">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE [ACETYL-TRANSFERRING]]-PHOSPHATASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="5">
        <location>
            <begin position="106"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="531" mass="59215" checksum="7B2ECFF508B61F89" modified="2018-12-05" version="1">MSSNMCTRLMCHVRSGTLSVCFSAGWYARCSSSCHYKVLHSQARAYSSHGRRSDGDSSQGRMQGLSTPVPELDFQLSRLQIDGVLRANEQSVRIPEFDRRGGPSPVLKFESNQLAANSPLEDRRSTATCLQTRGMLFGVFDGHGGHACAQVVSERLHYYIAVAMMSESGLEDLEAAMETLRPVPPILQWYKHHNDYNYRESASLYVNHLRVYWQELLASEEHGDGMSTSEALSYAFQRLDTDLSLEAQVPLANDLMHNTAVQAAFAGCTACVAHVGPDSIYVANAGDCRAVLGVQESDGSWSALPLSQDHNAANTAELQRVHSQHPSSEHQTVIVDDRLLGVLMPLRAFGDVRFKWSRELQQSVLESGGCDLEALNLYQYTPPNYLTPPYLEATPEVTYHRLRPQDRFMILASDGLWDELDNEEAVRLVAEHLTGIHLQAPVSASQRQISLGQMHQLLLRRRARATPALDLNAATHLIRHALGTNEYGEMDQERLAAMLALPDDLARMYRDDITVTVVYFNSSLNKQSRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5K3</accession>
    <name>A0A3B4D5K3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CFAP57</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldolase class II family. Adducin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5K3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018805"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028219.2">
        <property type="protein sequence ID" value="ENSPNAP00000018805.2"/>
        <property type="gene ID" value="ENSPNAG00000003897.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155257"/>
    <dbReference type="OMA" id="EIQVCAV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF5">
        <property type="entry name" value="GAMMA-ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="4">
        <location>
            <begin position="206"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="677"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="728"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018805.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="903" mass="102933" checksum="04CFE99F11E64FBD" modified="2024-11-27" version="2">MKSLVHRFYKIILCLGAEAVSHMTILQITHIHTLLSHAESPPPHRAPVRRSLSECSRVCAVERRRERAQCVCVRMSVSRADSRVSPPHSLELKQNYNSYSEKTHHPQSPTTASPDFRQDISMVEQRKRVTNTLQSPDFKDELECLVKEQSTKGGGIFTLNHIADLIINNTLTHSSTYSSNGVGSVSPVNDVGGLEWVSKAERQSRCKLASLYRLLDLYGCTQFTNAYITVRVCREQDHVLILPQGLSFSEATAASLMKVNLIGQVVEHGSTGLGVDDTAFKLHAAVYSARPDAKCVIHTHTPATAAISSMKSGILPISHEALLLGDVSYFSYHGELDDGQEMVELQKALGPTAKVLVLRNHGLLAFGETIEEAFHYTYHGQQACEIQVCAVLCAGSVENLCVVDRECVKVVPSAGGEAEDGEIRWKVGEAEFESLMRMMDNLGYRTGYAYRHPIVKVKPQNRNDVEIPATVTGVGQDEQDTFPWSSITLQGVRRDRGKMLWLNSPNSYTKIQDNTWTGSCKGTPIRIEDPNQFVPLNTDPTEVLEKRNKIREQNRVDLMTAGPKSQLLANIVIDTPPGPAFIYEDEEETAALPPNPFSQSDTELEEYTQSIENKLNQSEADDITDVEETTTTTYGSTVSLSFSPLKSPERGDALSALLLNEQDDDDVTLQVTEVSFSKEMEVSITTTVADNIPETEPTDGQSKSPKKKKKTFRTPSFLKMSKKKPREKGKDKGREKEKEKDREKSKEREKEKEKDKEKEKEKEKVKEKEREKSKEREKEREKEKEKEKEKEKVKEKEREKSKEREKEKEKEKEKEKEKEKVKEKEREKSKEREKEREKDKEKEKEKEKVKEKEREKSKEREKEKEKEKEKVKEKEREKSKEREKEKEKTSEKQKEKEDEKAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5Z4</accession>
    <name>A0A3B4D5Z4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">REEP2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547599.1">
        <property type="nucleotide sequence ID" value="XM_017692110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5Z4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018858"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028309.2">
        <property type="protein sequence ID" value="ENSPNAP00000018858.1"/>
        <property type="gene ID" value="ENSPNAG00000005024.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424222"/>
    <dbReference type="CTD" id="51308"/>
    <dbReference type="GeneTree" id="ENSGT00940000160001"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031883">
        <property type="term" value="F:taste receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF29">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="270" mass="30315" checksum="0695A5B165D69B8C" modified="2018-12-05" version="1">MVSWIISRLVVLAFGTLYPAYSSYKAVKTKNVKEYVKWMMYWIVFALFTTAETITDMVFSWFPFYFELKIAFVIWLLSPYTKGSSVLYRKFVHPTLSNKEKEIDEYITQAKDRSYETMMRFGKRGLNIAATAAVTAATKGQGVLSEKLRSFSMQDLTLIQNEEELQLDPTDDTHTAATLPRTKTVTRSVRAPPIHPEGESLPSSRSDADQSDSKTEHSDEDAADKVPKRTASVRAAKKPVAAKTETTKTVKKQPKKKSTTATSSNAAETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7X1</accession>
    <name>A0A3B4D7X1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tumor necrosis factor ligand superfamily member 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">TNF-related apoptosis-inducing ligand</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homotrimer. One TNFSF10 homotrimer interacts with three TNFSF10A mononers. One TNFSF10 homotrimer interacts with three TNFSF10B mononers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017536955.1">
        <property type="nucleotide sequence ID" value="XM_017681466.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7X1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019216"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028897.2">
        <property type="protein sequence ID" value="ENSPNAP00000019216.1"/>
        <property type="gene ID" value="ENSPNAG00000025766.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410411"/>
    <dbReference type="CTD" id="114466"/>
    <dbReference type="GeneTree" id="ENSGT01130000278318"/>
    <dbReference type="OMA" id="QSCQHPI"/>
    <dbReference type="OrthoDB" id="5980568at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00184">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000014">
        <property type="entry name" value="Tumor necrosis factor ligand superfamily member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017355">
        <property type="entry name" value="TNF_ligand_10/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471:SF28">
        <property type="entry name" value="DEATH LIGAND 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471">
        <property type="entry name" value="TUMOR NECROSIS FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038013">
        <property type="entry name" value="TNF10_TNF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0202">Cytokine</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 19" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="THD" evidence="21">
        <location>
            <begin position="148"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between all trimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038013-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="310" mass="34786" checksum="4FE78A6F840DABE4" modified="2018-12-05" version="1">MATQNNQDYFRSVSSESTTYMMVPANTRRESPSKLWIAMVVIVVVVLQIASTTGLFVYLNMSLSHVKSQGVTEELRCLGLLNALDKNRDIPEDLAQLFGEPCIKLAEGIKAYISKVTENIISKHTLQEARTMPRTYNTTGSKFVTQRPSAHLTLSSLQGITPLYPNPQPDLHQSCRHPVRSWANQSFGAHLHNMTLANGRLRVPQDGRYYLYAQVYFRYPSPSEGEQHSTSHQLVQCIYKKTSYLRPIQLLKGVGTKCWAPDAEYALHSVYQGGLFELRAGDEVFVSVSSPSMVHGEDSSSYFGAFRLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D897</accession>
    <name>A0A3B4D897_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Hexosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">B3GNT3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="11">Protein modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4D897"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019735"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039624.2">
        <property type="protein sequence ID" value="ENSPNAP00000019735.2"/>
        <property type="gene ID" value="ENSPNAG00000026410.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159134"/>
    <dbReference type="OMA" id="VSYLQGH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030311">
        <property type="term" value="P:poly-N-acetyllactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000009">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF23">
        <property type="entry name" value="N-ACETYLLACTOSAMINIDE BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019735.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="383" mass="44560" checksum="E1C40ADF27D0DCBA" modified="2024-11-27" version="2">MFRMFRRIRVRYWKLDMVALFIVGALIILLRTNIHDKNQEEPGYKWNLETDAGTPQAFSSLPPKCEQNSSVSNFSGFSSLPQHIQDFLYYRHCRHFPMLLNIPDKCGGPERSSDVFLLLVIKSSPPNYDRREVLRKTWAAERLQNGVWIRIVFISGTDGQSFEKQRLNKLLEAENRKHKDILQWDFHDSFFNLTLKQILFLEWMEKFCPNAHFLLNGDDDIFANTDNMVVYLQGLGDDGANKHSFVGHLIQYVGPIRNTASKYYIPVQVYESDRYPPYCGGGGFLLSRFTARTIYNMSHSINILPIDDVYMGMCLEKAGLKPSSHIGIRTAGLHIPSKALDPYDPCYYREMILVHRFLPHQIYIMWHEVHQQDLKCGKTLRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCP3</accession>
    <name>A0A3B4DCP3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">U6 snRNA-associated Sm-like protein LSm2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553513.1">
        <property type="nucleotide sequence ID" value="XM_017698024.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390663.1">
        <property type="nucleotide sequence ID" value="XM_037534766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCP3"/>
    <dbReference type="SMR" id="A0A3B4DCP3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020841"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031687.2">
        <property type="protein sequence ID" value="ENSPNAP00000020841.1"/>
        <property type="gene ID" value="ENSPNAG00000027769.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427683"/>
    <dbReference type="CTD" id="57924"/>
    <dbReference type="GeneTree" id="ENSGT00390000016597"/>
    <dbReference type="OMA" id="DNISCTD"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000009">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016394">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="95" mass="10853" checksum="623591A09BDABACE" modified="2018-12-05" version="1">MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVTDPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDTQLLQDAARKEAMQQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFU1</accession>
    <name>A0A3B4DFU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Eukaryotic translation initiation factor 4E family member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EIF4E3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571398.1">
        <property type="nucleotide sequence ID" value="XM_017715909.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFU1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021946"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033730.2">
        <property type="protein sequence ID" value="ENSPNAP00000021946.1"/>
        <property type="gene ID" value="ENSPNAG00000029186.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438222"/>
    <dbReference type="CTD" id="317649"/>
    <dbReference type="GeneTree" id="ENSGT00940000155865"/>
    <dbReference type="OMA" id="LPLQYHW"/>
    <dbReference type="OrthoDB" id="17977at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF66">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25345" checksum="336B3ED057582E37" modified="2018-12-05" version="1">MAAPAAPNLQLNAGRQSGSPVNSSENNIHIDEKELENITNHVEDGTSLPLHSPWTFWLDRSLPGTTAAECESNLKKIYTVQTVQSFWSVYNNIPAVSYLPLRCSYHLMRGERRPLWEEESNAKGGVWKMKVPKESTSAVWKELLLATIGEQFADYCASEDEVVGVSVSMRDREDVVQVWNGNASFANEANILGRIYELLPQISFKAVFYKPHEEHHAFEGGRSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DG71</accession>
    <name>A0A3B4DG71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anticodon-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">POLG2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017548504.1">
        <property type="nucleotide sequence ID" value="XM_017693015.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DG71"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022096"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040982.2">
        <property type="protein sequence ID" value="ENSPNAP00000022096.1"/>
        <property type="gene ID" value="ENSPNAG00000029339.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424784"/>
    <dbReference type="CTD" id="11232"/>
    <dbReference type="GeneTree" id="ENSGT00940000153759"/>
    <dbReference type="OMA" id="WGQEVLE"/>
    <dbReference type="OrthoDB" id="57698at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF8">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anticodon-binding" evidence="1">
        <location>
            <begin position="327"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="420" mass="47340" checksum="922B843BE6576DA2" modified="2018-12-05" version="1">MLTYCMRKRLFLLSREPLKAAVSPQQRRCESGGSVLDLQHFVSSGGTGRRSTYGPLGTELKRNVLQQWWTAVVSLQPQVFGISTPCSSTERRTLKTEDGEALGEQLKLFQHDQSLRTSLLQGALEQFVPSLELLNKKLPFGLAEIGLCYQPEPERAGTSGCFSEVTKASLVWFCSFRTSSQWLDYWARQRLQWWRKFALGPSDFSVCDVLSEELEEGASRGVKVLYQFPSGSETLETLWSFGDTKLLKTHHGSRAKLQCREGRTSVVPHVLSVSANMDKGVLAFLYNSLQPVQKVDSKQRLHQRKVLKLHPTLAPVKVALDMGRGSTSELRQVCEGLLQEFLEAGIYSWPGYLDSTYSSMQSLHAKYDEMGVLFTVLITDITLENGLLMVRNRNTTVKETMHISEIKQFLLKYISAAENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DH48</accession>
    <name>A0A3B4DH48_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DH48"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014180.2">
        <property type="protein sequence ID" value="ENSPNAP00000022825.2"/>
        <property type="gene ID" value="ENSPNAG00000007389.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287108"/>
    <dbReference type="OMA" id="SEPYTQK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47485">
        <property type="entry name" value="THYLAKOID LUMENAL 17.4 KDA PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47485:SF1">
        <property type="entry name" value="THYLAKOID LUMENAL 17.4 KDA PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022825.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="265" mass="26379" checksum="74704E5597C95CE8" modified="2024-11-27" version="2">LASACLGSVCFDSICFNSVCFDSVCFSIVPVSVVACLGSACFGSACLSSVFLAGACLGSACFGRICFNSACLGSACLGSACLGSACLGSACFSSDCFVNVCFNIVCFGTDCFNGACFGSACLSSVCLASVSFNSACFSGVCFNSVCFGSACFGSACFGSACLSSVCLASVFFNSACFRGVCLASACFASACFASACLVSACLVSACLGSAYFRSACFGGVCLGSVCLSSAWFGSVCFDCVCFSTDCFNSVCFGTNCFNGACFGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHS3</accession>
    <name>A0A3B4DHS3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nck-associated protein 5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHS3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013763.2">
        <property type="protein sequence ID" value="ENSPNAP00000022993.2"/>
        <property type="gene ID" value="ENSPNAG00000000289.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063607"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032769">
        <property type="entry name" value="NCKAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026163">
        <property type="entry name" value="Nckap5l"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21740">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21740:SF3">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15246">
        <property type="entry name" value="NCKAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nck-associated protein 5 C-terminal" evidence="3">
        <location>
            <begin position="798"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="538"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="611"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="722"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1067"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="884"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="547"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="636"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="666"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="733"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="791"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022993.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1165" mass="127075" checksum="754BEA83D1448AC7" modified="2024-11-27" version="2">IHTAENTALAQANENQREAYERCLDEVANHVVQALLNQKDLREECIKLKMRVFDLERQNKTLTELFTQKLSLLLLLFPISPPTTSVPPSSPVSSVVELFNDECTQNGTGGSPQASASSMEAMSPFLKKKAHILEVLRKLEESDPLKFHPSACYYYHGQALLPGETTVVTPSTRQPHCRHSSSDSDIHEYANGEGAQQDSRASLRYGGCPSCRILSQRSSLDSLLKCNQVTMGSHSGRDESQTSSEEHSASAQSTHPQTKMPDKANQPSTHNRANVTHFSQPKATVPYLSSLTADSSEIGEEPKHDRDKISPTKSKGANSGSQDVRQCDSIPTRADECRYLEVATDSVKHEHDSSVCNGVFSSNEASVSKKASVESYSPTTSAIVQQQATGKGKFLHSPTSPPSSFSESKPSPVSSPSKLLKFLKIPSMAENTPTANALRLSPQLTRSSKIPCRNNYEVYQSPVMSRKATTTERDKPSSASKTDTYPGTSQSSSQPHKGTQVPHYENILDPSASHSINGFPQITPEEMDLSDKQKISLRDERLLSPLSLKTSDSSATTGESPSDPSDQDTDTESPVWQRPQHHFSLDKQPSLPKRTVTKRLAALGKLKSTEDLQQAVEKREVQSNMGKTGASPGNNERSKTAERIAMEQRHSKYTDSLDGKPYPKTSFGNYSKASGSSSHEQSTKPVTAEKNYIAKVEGQKIKMGMSSTSSCETPVVMRSYGKCPNPLTHHSKTVPSPQSSPTRVQSKSPSKAGNASSYPRGMKPVQDERSHPQRHTSQSANIKTVEEKRPSAAAPAPQSAIEQKVMRGIEENMLKLQEQDRGQAAETKQKTSNGIASWFGLKKSKLPALNRKPEMSKLKINVSSSSGAKDASGAKGGPRKVVESLNISKLMEKAEDLRKALEEERAYVNRVGIALDRPGRGHSCEVVMDQAQGQLSLMYRGVTADSFMQQLLNSYTSFQTYNAELSSHSGRMDRNTCGCFQILSSLIFLSVKDESFKCCLSANGGRFGELQGCLTFLKEPAVEMFSNRSDPQLFCVCCPPGSGSSMRTLDSGIGTFPLPDYASSAAGKGIPKPQGDQGFSTSQGKQGALLKAPRKARTLERELSSLDEVNPCVLYTSPLEAKGPAMHLSSTIHEGVGQNWLYQNVFNTLSNSATLFSLCKRKSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKC2</accession>
    <name>A0A3B4DKC2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017570461.1">
        <property type="nucleotide sequence ID" value="XM_017714972.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570462.1">
        <property type="nucleotide sequence ID" value="XM_017714973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKC2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023908"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011470.2">
        <property type="protein sequence ID" value="ENSPNAP00000023908.1"/>
        <property type="gene ID" value="ENSPNAG00000008394.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437700"/>
    <dbReference type="CTD" id="564943"/>
    <dbReference type="GeneTree" id="ENSGT00940000154996"/>
    <dbReference type="OMA" id="MVAREAT"/>
    <dbReference type="OrthoDB" id="10061535at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000014">
        <property type="entry name" value="Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF178">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IMMUNOGLOBULIN-LIKE DOMAIN-CONTAINING NOGO RECEPTOR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017413324" description="Ig-like domain-containing protein" evidence="13">
        <location>
            <begin position="30"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="550"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="404"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="615" mass="69585" checksum="489C743A8CB10242" modified="2018-12-05" version="1">MVAGEASGHSYLVACWQPILILMLGTVLSGSTTGCPSRCECNAQERSVVCHRKKLMSVPEGIPSETRFLDLSKNRIKTINPEEFSGFPNLEELELNENTISTIEPGAFNNLYGLRTLGLRSNKLKLIQLGVFTGLSNLTRLDISENKIVILLDYMFQDLYNLRSLEVGDNDLVFISHRAFHGLSSLEHLTLEKCNLTSVPTEAFTHLHSLNTLRLRNLNINSIRDYSFKRLYRLKVLEIANWPYLDTMTTNCLYGLNLTSLTIASSNLTSIPYVALRHLVYLRFLNMSYNPIIIIEGNKLHDLLRLQELHLVGGRLAMIEPYSFRGLNYLKVLNVSGNSLTTLEESVFHSVGNLETLVLYDNPLACDCRLLWIFRRRWRLNFNRQQPTCASPEFVQGKEFKDFPDILQPNYFTCRKSRIRERKAQQRFVDEGTTVQFVCQADGDPAPVIMWLSPQKQFITTKTIGRLTVFPDGTLEVRYAQIQDNGTYVCIASNAGGNDTALAHLHVHSYSPDWPNQPNKTFAFISNQPNDSGANGTKETVPFPFDIKTLIIATTMGFISFLGVVLFCLVLLFLWSRGKGNTKHNIEIEYVPRKSDAGISSSTADAPRKFNMKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQ47</accession>
    <name>A0A3B4DQ47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQ47"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007204.2">
        <property type="protein sequence ID" value="ENSPNAP00000025603.2"/>
        <property type="gene ID" value="ENSPNAG00000010789.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156523"/>
    <dbReference type="OMA" id="WESFNVF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF8">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="73"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50042"/>
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            <dbReference type="Ensembl" id="ENSPNAP00000025603.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="402" mass="46623" checksum="F33270AE4E0F267B" modified="2024-11-27" version="2">MERPAPGGRERFSWDGWKNLLLPQLNRQSLYVYGSEVAVERDSNSCLFRSYYTMCMVAITFLNLIGIPIEIAFLDGQTGVGWEGFNVFSDTLFLIDVGLNFRMGIIAEDSEAAILDLKCIRQNYLKSWFIPDVIAAFPVGYIILIDLQYHSDSPSSKASKMVRILMFVRILSLVRLLRVSRLVRFFNEVSNANLEVVRVFFRILSLFMMIFLLCHWNGCVQYFVPMLEEFPSDCWVRRENLMVLMGEKYSFGIFRALSHMTAISYGSSETPTISYVFFFLFLLKVPMFKNCDVNFISSIVSELQYEVFQEGDIIMCQNTPGDRMFFIEHGQVVVETDSFQQELCDGDYFGETCLLIRGRRLSTVQAQTICQLFSLCVDSFYRVLRDYPEVMTDIYREHLSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQV3</accession>
    <name>A0A3B4DQV3_PYGNA</name>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Characoidei</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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        <property type="gene ID" value="ENSPNAG00000006414.2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CD34/Podocalyxin"/>
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    </dbReference>
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    </dbReference>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="328" mass="35597" checksum="13F061CF52F20A42" modified="2024-11-27" version="2">METLMSLKMRNGYRVMALASVIGLVFFQGVTCDTALEPHTMRSKSAPSLQAAHVRGDNLHPTQAESKSHDQVQVLPTIQDQTTVQSATGPEVKQDGTTQSRISTTVAGPAAQQDTQAPQHITTNQPDATPSIKHMDQVVCVEEAAVRGKEAVRLKLKASSSCEENKAKIQSILEHLCHDDSKLEIYQRDHTDEMIISGQCIKDDAKGMTKKFNNDNIKDKVGVVEAVPILVKHSRTVLITVLLAGLLLAALLIVAYILKTHRTQAKGVRLAEESFQVDEQNQGNTLLSVAPLPPQEPLDKPTSNGESPELPPTNGHSATQTPVADTQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DWZ8</accession>
    <name>A0A3B4DWZ8_PYGNA</name>
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        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Characiformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
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                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017539793.1">
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    </dbReference>
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        <property type="gene ID" value="ENSPNAG00000033843.1"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORY RECEPTOR 530-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
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        <location>
            <begin position="48"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027514.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="328" mass="37162" checksum="67C83EA2A37374B4" modified="2024-11-27" version="2">MSTLTSSLLQNISVVRPEYFYISGFSGIPFVKYYFIFLFVTYFVAVFGNSFVLFMIFTDRSLHIPRYMGIFNLALADFGETNALIPNLMKTFLFDSQYISYNACLANMFFVFFFLSVQSLTLVVLAYDRYIAIRLPLRYHSIVSNSLMTAALVSVWSICTVVVGTMVGGISRLSLCKTNEIKSYFCDHGPIYNNACNDNTMSSVMAKLWTALFLYFPLVAVSLSYIGIFLELTKITTWEGRLKALKTCFTHMLLVGIFIVPIISTYIAALTFTLHPNARIINTSLASAIPPMVNPIIYVLNTKEFKDFIVKMFKIKIKSKLKVQALPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DY87</accession>
    <name>A0A3B4DY87_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Interphotoreceptor matrix proteoglycan 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Sialoprotein associated with cones and rods</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Chondroitin sulfate-, heparin- and hyaluronan-binding protein. May serve to form a basic macromolecular scaffold comprising the insoluble interphotoreceptor matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Photoreceptor inner segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
            <location evidence="3">Extracellular matrix</location>
            <location evidence="3">Interphotoreceptor matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017553197.2">
        <property type="nucleotide sequence ID" value="XM_017697708.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000028390"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000100.2">
        <property type="protein sequence ID" value="ENSPNAP00000028390.2"/>
        <property type="gene ID" value="ENSPNAG00000014058.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427501"/>
    <dbReference type="GeneTree" id="ENSGT00530000063503"/>
    <dbReference type="OMA" id="HHNETGE"/>
    <dbReference type="OrthoDB" id="8960773at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033165">
        <property type="term" value="C:interphotoreceptor matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039861">
        <property type="entry name" value="IMPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12199">
        <property type="entry name" value="INTERPHOTORECEPTOR MATRIX PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12199:SF3">
        <property type="entry name" value="INTERPHOTORECEPTOR MATRIX PROTEOGLYCAN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="13" id="KW-0373">Hyaluronic acid</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0730">Sialic acid</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043994404" description="Interphotoreceptor matrix proteoglycan 1" evidence="18">
        <location>
            <begin position="21"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="19">
        <location>
            <begin position="326"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="19">
        <location>
            <begin position="1476"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="164"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="551"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="659"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="887"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="566"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="688"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="768"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="801"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="820"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
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            <begin position="910"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="964"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
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            <end position="995"/>
        </location>
    </feature>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1017"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
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            <begin position="1065"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1112"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1122"/>
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        </location>
    </feature>
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        <location>
            <begin position="1146"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1367"/>
            <end position="1376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004504"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004593"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022981"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028390.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DYJ8</accession>
    <name>A0A3B4DYJ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exosome complex component RRP4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Exosome component 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EXOSC2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC4 and EXOSC7. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6. Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. Exo-9 is formed by a hexameric base ring consisting of the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and a cap ring consisting of EXOSC1, EXOSC2 and EXOSC3. The RNA exosome complex associates with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with GTPBP1. Interacts with ZFP36L1 (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRP4 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017701643.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DYJ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028533"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014804.2">
        <property type="protein sequence ID" value="ENSPNAP00000028533.1"/>
        <property type="gene ID" value="ENSPNAG00000014281.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429699"/>
    <dbReference type="CTD" id="23404"/>
    <dbReference type="GeneTree" id="ENSGT00940000153596"/>
    <dbReference type="OMA" id="GVNGFIW"/>
    <dbReference type="OrthoDB" id="1650at2759"/>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003407">
        <property type="term" value="P:neural retina development"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0009259">
        <property type="term" value="P:ribonucleotide metabolic process"/>
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        <property type="term" value="P:spinal cord development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22525">
        <property type="entry name" value="KH-I_Rrp4_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000022">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000038">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
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        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21266">
        <property type="entry name" value="S1_RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="14">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="RRP4 S1" evidence="16">
        <location>
            <begin position="76"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="15">
        <location>
            <begin position="170"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="294" mass="32727" checksum="6FBF26B069601871" modified="2018-12-05" version="1">MAVDMRLPTVRKQISLSGSQSTDGKHLVVPGDVITSDTGFMRGHGTYMDDEKLTASVAGEVERVNKLICVRPLKTRFNGEVGDVVVGRITEVQQKRWKVETNSRLDSVLLLSSVNLPGGELRRRSAEDELAMRDYLQEGDLISAEVQSVFSDGALSLHTRSLKYGKLGQGVLVQVSPSLVKRQKTHFHNLPCGASIILGNNGYIWLYPTPGQQDEEAGGYYTSLEPVPLPDREVISRLRNCLLALAAHKVLLYDTSVLYCYESSLSHQMKDILKPEIMDEIVMETRQRLLEQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DYZ7</accession>
    <name>A0A3B4DYZ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DYZ7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015040.2">
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        <property type="gene ID" value="ENSPNAG00000014429.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="9"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028645.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="166" mass="19044" checksum="BD4C4317851E849B" modified="2024-11-27" version="2">MLKVNLCKPTMNAEFFNFISAGCDLVKTAGIEEVKFSGESVVLLCSCTDLQTKPNTVKWEFSSETYDFEEIYSTQTGLYRCSVQHDFKNIRLHVKGKLFLFSVMIVGMYFVYKGYIAYIELFVFMCLVEFISAVRETPSHLWTTDPTTTPSEDPLTTAMNENERIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZW1</accession>
    <name>A0A3B4DZW1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017565571.1">
        <property type="nucleotide sequence ID" value="XM_017710082.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZW1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029942"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035849.2">
        <property type="protein sequence ID" value="ENSPNAP00000029942.1"/>
        <property type="gene ID" value="ENSPNAG00000016362.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434737"/>
    <dbReference type="GeneTree" id="ENSGT00390000006290"/>
    <dbReference type="OMA" id="CKLQFTF"/>
    <dbReference type="OrthoDB" id="9934809at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027897">
        <property type="entry name" value="DUF4559"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35083">
        <property type="entry name" value="RGD1565685 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35083:SF3">
        <property type="entry name" value="SI:CH211-91P5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15112">
        <property type="entry name" value="DUF4559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="316"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="451"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="677"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="544"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="769"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1145" mass="129780" checksum="0AD3A142C84F4DF9" modified="2018-12-05" version="1">MDSYQRFSDENYKNWLKAAESLYILRNYIRDFVEKETETYHSSLRETLKGGTCAKKCKKSPLCEKCNLWKEEILRRRVGKGALVHWENVEPHRWPTEKWEVAKVYMPRGQSGHRRFEEFDIAAILNFMAQCKHFKKFHQGQLMTKVINLRNTVMHSPEFRLNKEKMSNGRNNVLLLAELLGTCIPKLHETISEEIKQFDTVLENYPGQVSQVNFKTENLKLLEREQQALKEKIEFLAQRYDADQQIEIKEELQGMKNFLDQNKDLLESLRPQVNRLNEIQETVEKHEFQLDNLNNRVGNLEKVTHDPMFSDDPLKFKNHLIEQARKRKWPEPLFTEELDANGYRGRVVVNGRTFTGLQVCNNKRRAHQEVAKIALENLKSEFEDTEEASTSASLISSTSSTSSLFYGTVAVVLSKEVCSDGFVKEDEARESAYTNLARQFGLNDPGTDSTFRTVVLEHLAKCGIQPPLEITSQQDDKNFCKLQFTFCDKDGSTTKRKAEQQAAKVALQQLSGFFNCSSDDVPDENFKGFLKERLDALGMVNPVYKAEQNKPPEDAGTSSTSRDAEHISSTQPTHDQENTSLQPKDSLAVVQPPPTTPSGCAAVGLKMEACDSCPAPIPREVIMEDHTGNLFYGTVAVVLSKEVCSDGFVKEDEARESSYTNLARQFGLNDPGTDSTFRTVVLEHLAKCGIQPPLEITSQQDDKNFCKLQFTFCDKDGSTTKRKAEQQAAKVALQQLSGFFNCSSDDVPDENFKGFLKERLDALGMANPVYKAEQNKPPEDAGTSSTSRDAEHISGTQTQKHGKEKDSVAVVQPPPTTPLDCVIGLTTETRDSNPSLIPREVDLQDHTEIKSLLAVYHLKPPSVTVERVCTNQNFSLTMNINLDKFTFKNSNVYTSKKDAIRKTYYSLGCALGVFQKDTDESKATMLVKQDFSQKSLTHPKEDIKDDKNFCCSLTEITYSLVCDGQGSSEAKAKQDALQKALCTLPLLFGHEALPASSSTEETEFQINTLLRNACQKDLAFSLKPNQHNISVDLRFKDFIMASKSQRTKKENRNLLSKRILGLLGEETEPHCPSLRNCLDDWFKQRNLQQPVFEDTDEAQGSKATFSVELSFRHPDWEDSMENAKKNLVQELEKRFKYLIDQSKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0W0</accession>
    <name>A0A3B4E0W0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559862.1">
        <property type="nucleotide sequence ID" value="XM_017704373.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000028946"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015575.2">
        <property type="protein sequence ID" value="ENSPNAP00000028946.1"/>
        <property type="gene ID" value="ENSPNAG00000014823.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431311"/>
    <dbReference type="CTD" id="555848"/>
    <dbReference type="GeneTree" id="ENSGT00940000156919"/>
    <dbReference type="OrthoDB" id="10249045at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03868">
        <property type="entry name" value="M14_CPD_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000026">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000043">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF57">
        <property type="entry name" value="CARBOXYPEPTIDASE D, B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017475744" description="Peptidase M14 domain-containing protein" evidence="12">
        <location>
            <begin position="25"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1228"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="36"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="457"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="13">
        <location>
            <begin position="861"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1298" mass="144479" checksum="5281EC5EF49B9FED" modified="2018-12-05" version="1">MMSNVWFTLSRLFAILHVVHYVTCTPESDSEETYNKYYNYAGLTRRLWSFVEKYPHICSLESVGKSAEGRELWVMRVTAHSERNEPGKPRFRYVGNMHGDEAVSRQVLVYLTEYLLTRYGTDPRVTELVNSTDIYIMPSMNPDGFEKATEGDCTGSSAARQNAKHVDLNRGFPDQFDQLPRVLEGDAPEVTAVMSWILHKKFVLSGNLHGGSVVASYPFDDSSSHIASGVYSHSPDDALFRYLAQTYAENHPIMKEGRPRCPYDPDEGFKDGITNGAAWYDVPGGMQDFSYLQGNCFEVTFELSCCKYPFASELHTEWKNNREALLTYMEKVHMGVNGYVMTSAGIGLPGANISVAGNNHNITTWIFGDYFRLLLPGTYNITASSSGYLPKTVNNVTVTEDKATLLNFILNTSSEKMQMPGFLASPVPSTAFTPTVRIFDNQGASTPEPLVHPQDIRHHSYLDMELFLQQINTVHSSITHLYSIGQSAQGRELYVMEISTNAGIDQPGKPEIVFVGNLHGSDAVGKEMLLILVEYLCSNYGSEALVTRLVNSTRVHILPSMNPDGYEMVFEAQKYFGTNSDFSGGVRYSSNQNTDLSKNFPDQSYGEDFVQPEKAAVMSWIKTHSFVLSASVLGGMIGVKYPNSVDSLDEDVFKLIAEACFVKYPFLQLSQACDLPRPKDRNYIKPSAPGIDMQTWAYRNTGTLGFTIGLSCDLYPQAKDLLTYWKRYHVVLLQFIQQVHFSVRGRVTDVQSGQGIVNSTIVVEGSRHHVHTSKSGQYWRPLAPGAYKIQAFAPGYSAVVVPVRVLETQVEQVDIGLSPEPLAHSEGQLEGEEFQKLLEDLSSTSGLEQLVQSLLPGGMLHYRTYRERSDFLQELTLTFPQITRLYSLGHSWEFRTIWALEITGNLESPQPTEPKMRYVAGVHGNAAVGPELLLEFAFVLCINYGGNPTITKLVDRSRIVIVPCVNPDGRELAQEGKCFSTAGLTNAHGVDLDTDFLSGSLSVQPETRAMMDLIREGGFSLSVNLEGGSLLVTYPYDRTTQPAHNEETLKYLAVVYAGNHLGMHFGYPGCHIGPESVPGGILRGAEFSNHSGSMKDYSMDVSLCPEITVYTGCCLYPPAEQLLSLWVEHRTSLFAMLLEVNKGLSGVVRDKEGQPVSDAVVRVNGSVLVHSDTQGYFHTLLPPGTHQLEIHAQGFQQQLWKVNVSSHQMASPLVIEFTENSTHFSQGVVLAAATSIITILLFSLLIWHFRSAKFVNGVRWLRQENLQMDPIVSEKSPLRGVFLEESESEDDAFYLEQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4Y7</accession>
    <name>A0A3B4E4Y7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">IL12B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4Y7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018222.2">
        <property type="protein sequence ID" value="ENSPNAP00000030294.2"/>
        <property type="gene ID" value="ENSPNAG00000017202.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271828"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07698">
        <property type="entry name" value="IgC1_MHC_I_alpha3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000001">
        <property type="entry name" value="H-2 class I histocompatibility antigen, alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="191"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030294.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="299" mass="34335" checksum="DC4B2A6D98215BE5" modified="2024-11-27" version="2">TEMLTVFVSTAFHSLQYIITATTPGIDFPEYIVVGLVDEEQFVYYDSNIKKMIPKTEWMQKNVREDYWTSETQKQDRVQEIFKANLAILMKRFDQTDGVHTLLRRSGCNLDKNGSVTGYFQFNYDGEEFLILDLKTLTWTAAHQKALGTKLKWEAAGDPHFRKHYLDNDCVDYLRKYVEYGNSTLGRKVSPEMSLSQKDSSSPVVCHATGFFPKAVMISWQKDGEDLHKDVELRETLPNQDGTFQKRSVLTVSPEELNRHNYTCIIQHSSLEEQVVLLVSDLSGGTNLLSLSYCYSLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E5A1</accession>
    <name>A0A3B4E5A1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L14, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRPL14</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550903.1">
        <property type="nucleotide sequence ID" value="XM_017695414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5A1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030409"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018442.2">
        <property type="protein sequence ID" value="ENSPNAP00000030409.1"/>
        <property type="gene ID" value="ENSPNAG00000017105.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426131"/>
    <dbReference type="CTD" id="64928"/>
    <dbReference type="GeneTree" id="ENSGT00390000001121"/>
    <dbReference type="OMA" id="RCIHVYT"/>
    <dbReference type="OrthoDB" id="274765at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000004">
        <property type="entry name" value="39S ribosomal protein L14, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L14, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="145" mass="15875" checksum="30215F42A4C564C4" modified="2018-12-05" version="1">MAFSQMMLCKVPAAAAAAHYRSFSVSAAVAAIQKLTRVRVVDNSPLGNTPYHRPPKVIHVYTKNGVGKVGDMVLLAIKGQKKKALIVGHKMPGERMTPRFDSNNVVLIEENGNPTGTRIKAPIPTHLRRMEGDYSKLLAIAQRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E5S1</accession>
    <name>A0A3B4E5S1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein tweety homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TTYH1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Probable chloride channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamate(out) = L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:66336"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:66338"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homotetramer; disulfide-linked. Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cell membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the tweety family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570074.2">
        <property type="nucleotide sequence ID" value="XM_017714585.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570075.2">
        <property type="nucleotide sequence ID" value="XM_017714586.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5S1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019788.2">
        <property type="protein sequence ID" value="ENSPNAP00000031110.2"/>
        <property type="gene ID" value="ENSPNAG00000018263.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437476"/>
    <dbReference type="GeneTree" id="ENSGT00950000183060"/>
    <dbReference type="OMA" id="DFCSEPN"/>
    <dbReference type="OrthoDB" id="187568at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030868">
        <property type="term" value="C:smooth endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072320">
        <property type="term" value="F:volume-sensitive chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF5">
        <property type="entry name" value="PROTEIN TWEETY HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04906">
        <property type="entry name" value="Tweety"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 17" id="KW-0868">Chloride</keyword>
    <keyword evidence="10 17" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="481"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031110.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="513" mass="56220" checksum="AFC39941A2D2DCC2" modified="2024-11-27" version="2">MATVPSYSPSIWVRMCHALPRFDLTMQMRDNVFTPDSWEYQQTLLVLSSLSAIALVLSLLVVLSFLIHYCCCHRGDAGRDAEEEEEDDDASGGHGYSGKKGRSICCVTWVSVAAVTLCCVAIGVGFYGNSEANDGMYQLTSSLLTANYTLASIDLLVWDSVAVLQRSVSGPLTSLEDVFGGNKPALVSTRSCRRLSERVISLLSSLSLGRGVSSVSPGSLGANGTDSMVGVLTPAPPSIAPTLTPTSTSNSIPAPFSPGWAANTLMVNEDYRWLSYVLLLLLDLVVCLFILLGLAKQARWLLILMTVLAWLALFLSWGSLGLETATVVALSDFCFDPNSFVINSTHFNTGTSTEILDYYLTCNRKMTSPFQQLLTQSQRALSSIHSHLSGLERDALPRFPKAEKSLREVQQILNVTEGNFHQLVALLNCRGLNKDYIDSLKGLCYDGMEGLLYLSLYSFLSALAFTAILCSLPGAWRSFSSDSEEYEDSDSESEDPFTSHQDSKRFLQWQPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E603</accession>
    <name>A0A3B4E603_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E603"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030751"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036317.2">
        <property type="protein sequence ID" value="ENSPNAP00000030751.2"/>
        <property type="gene ID" value="ENSPNAG00000017646.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204738"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007281">
        <property type="term" value="P:germ cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF76">
        <property type="entry name" value="DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="8">
        <location>
            <begin position="16"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="6">
        <location>
            <begin position="16"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030751.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="145" mass="15050" checksum="46872DF40C3A1D2D" modified="2024-11-27" version="2">MNSKDNTLKKARSPKCARCRNHGFAVELKGHAGKCPFLACACWKCSLITKRTKIMASHRRIRGGLPSDAGKTAAGGANGDVPGSGSRAAGDASKRVADTGSSAVPQHSVRGTRGLDGAVTRGKVTVNGRVVGDLHDIILNPCRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7C3</accession>
    <name>A0A3B4E7C3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LSM11</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017553901.1">
        <property type="nucleotide sequence ID" value="XM_017698412.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017553902.1">
        <property type="nucleotide sequence ID" value="XM_017698413.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7C3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031670"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020853.2">
        <property type="protein sequence ID" value="ENSPNAP00000031670.1"/>
        <property type="gene ID" value="ENSPNAG00000019128.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427864"/>
    <dbReference type="CTD" id="134353"/>
    <dbReference type="GeneTree" id="ENSGT00390000012944"/>
    <dbReference type="OMA" id="QETSECA"/>
    <dbReference type="OrthoDB" id="10002367at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005683">
        <property type="term" value="C:U7 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071209">
        <property type="term" value="F:U7 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01739">
        <property type="entry name" value="LSm11_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039267">
        <property type="entry name" value="Lsm11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034109">
        <property type="entry name" value="Lsm11_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="161"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="343" mass="38189" checksum="3366C28913C16470" modified="2018-12-05" version="1">MEENEREAVSESSSAVCGQTAALNAGGQQVEQDRGGNTEADEDDVDKKLDISSELFDPLLALYSPQVPLPFPNIRCFNNVAEYESFIKGGRGRAKPENVEKKLRKSRRGVADPERIERLKKLMVNNPVKDGEQTGGKQSRRKKARKNVLTRMPLHEGSPLGELNRCVQERIRVKVHIRSFKGLRGVCTGFVVAFDKFWNLAMVDVDETYREPLLGEAFYHEKALTVTRLFQKLQVQEGQDLSTGQKDSGKAAATNTSTPADHSTEPHYTTRSSIKSNRGQRQQLCGKGPADSATAAIHVPDAGSKQRTKVPKPNVVYGRVRTRHVNQLFIRGENVLLVNVQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7K9</accession>
    <name>A0A3B4E7K9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ZDHHC24</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017553839.1">
        <property type="nucleotide sequence ID" value="XM_017698350.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7K9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032602"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022660.2">
        <property type="protein sequence ID" value="ENSPNAP00000032602.1"/>
        <property type="gene ID" value="ENSPNAG00000006687.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427839"/>
    <dbReference type="CTD" id="254359"/>
    <dbReference type="GeneTree" id="ENSGT00940000164006"/>
    <dbReference type="OMA" id="RMHLTWL"/>
    <dbReference type="OrthoDB" id="302728at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="101"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="291" mass="33309" checksum="9DCE0C8C836B8D62" modified="2018-12-05" version="1">MVTLGSRVWCRVEKTCRHLPIVLNTVLVFSITAEVSYLVVVEAPLEPELKKSDWSSTWKSIHLFAQYFMLVNITWNALLFLKTNPSIRGVFLGGDMVGQGWRYCYTCETHTPPRCSHCYDCNVCVLRRDHHCVFFGQCVGFRNYRYFLSCLFFMWAGLLYAVVMNAEVFIVILKEGVTFHSFMLLLVPWIMLVTGQVTAQAFAFAFIADTCVIGFLLVSAFLFFHVGLMVRGQTTREWYSSRRPYSLGILANIRECLGECWFLCWICPLIPSVLPGDGIHFKVTGSLEPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ED80</accession>
    <name>A0A3B4ED80_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dehydrogenase/reductase (SDR family) member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">DHRS1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ED80"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033191"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037778.2">
        <property type="protein sequence ID" value="ENSPNAP00000033191.1"/>
        <property type="gene ID" value="ENSPNAG00000021625.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157797"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd09763">
        <property type="entry name" value="DHRS1-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44147">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44147:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="34357" checksum="739E7A23A701E897" modified="2018-12-05" version="1">MALSGWVCVVTGASRGIGRGIALQLSEAGATVYITGRQMKTLQQTAAEVNERGGQCLAVVCDSSKEDDIKELFERVQREQNGRLDILVNNAYAGVQAIFDNMEKKFWEMDPKIWDDINNTGLRGHYFCSVYAARMMVAQGNGLIVTISSMGGLRYLFNVPYGVGKAACDRLAADTAVEMKSRGVASVSLWPGAVQTELVSQHIIEKEKPPGFDQKLKDVYNTGETTELSGKCIVELAKDKNLMSLTGKVLFTCDLARRYGLKDVDGRRVVDYTSWKFLLSQSPYMSWLSIITPSFIRVPRFIFSLAISKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EDF8</accession>
    <name>A0A3B4EDF8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gfo/Idh/MocA-like oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017565415.1">
        <property type="nucleotide sequence ID" value="XM_017709926.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EDF8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033865"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038203.2">
        <property type="protein sequence ID" value="ENSPNAP00000033865.2"/>
        <property type="gene ID" value="ENSPNAG00000018250.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434639"/>
    <dbReference type="GeneTree" id="ENSGT00390000004367"/>
    <dbReference type="OMA" id="LICERQT"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF2">
        <property type="entry name" value="BINDING ROSSMANN FOLD OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G00560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="140"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033865.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="47381" checksum="00276BA1A97422D9" modified="2024-11-27" version="2">MPSPVRVIVVGAGSRGETYSDYASIHPERMQVVGIADPRSFARSKLQKRHKVADENTFDDWSCVAEREKFADAVFICTPDRLHKDPAVALAKKGYHILLEKPMAVTLEDCREIAEACIQSGVILTVCHVLRYDPVISKIKTLIDCGVVGDVIHIQHLEPVGFYHFAHSFVRGNWRNEAESSFALLAKSCHDLDLIHHWAGGRRCIKVSSFGSLSHFRKENKPRGAANRCLDCSVESNCPYSAKKIYLDRVKEGWVRWPVSVICSNSVPDIESVTEALKTGPYGRCVYECDNDVCSNQVVNMEFEGGLTASFTMVAFTEEICERKTSICGSRGELSYNGKEISVFDFLTQTSTKHTVHKNVPGAFNSGGHGVADYHLVDAFISAVASEDPLLIQLGPKETLASHRLVFEAERARLENRVVFCETDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EE04</accession>
    <name>A0A3B4EE04_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor, arginine/serine-rich 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May play a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017543693.1">
        <property type="nucleotide sequence ID" value="XM_017688204.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EE04"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033529"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037990.2">
        <property type="protein sequence ID" value="ENSPNAP00000033529.1"/>
        <property type="gene ID" value="ENSPNAG00000022241.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415197"/>
    <dbReference type="CTD" id="406288"/>
    <dbReference type="GeneTree" id="ENSGT00940000155585"/>
    <dbReference type="OMA" id="KMDGNRS"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12597">
        <property type="entry name" value="RRM1_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12767">
        <property type="entry name" value="RRM2_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000053">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000170">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034520">
        <property type="entry name" value="SRSF1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029538">
        <property type="entry name" value="SRSF1_RRM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF66">
        <property type="entry name" value="SERINE_ARGININE-RICH SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="117"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="187"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="244" mass="27658" checksum="ABB50581664C09BE" modified="2018-12-05" version="1">MSGGVIRGPAGSNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYARDGYDYDGYRLRVEFPRSGRGMGRGGFGAGAPRGRYGPPSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKMDGPRSPSYGRSRSRSRSRSRSRSRSNNRSRSYSPRRGRGSPQYSPRHSRSRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFQ5</accession>
    <name>A0A3B4EFQ5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Secretin receptor</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFQ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034568"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026496.2">
        <property type="protein sequence ID" value="ENSPNAP00000034568.2"/>
        <property type="gene ID" value="ENSPNAG00000023907.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156402"/>
    <dbReference type="OMA" id="SHEYFMW"/>
    <dbReference type="OrthoDB" id="5967113at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015055">
        <property type="term" value="F:secretin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15275">
        <property type="entry name" value="7tmB1_secretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047037">
        <property type="entry name" value="Secretin_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF13">
        <property type="entry name" value="SECRETIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="304"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="12">
        <location>
            <begin position="6"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="13">
        <location>
            <begin position="102"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034568.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="407" mass="47471" checksum="EC4488A3336273F7" modified="2024-11-27" version="2">PSFSPSCLQDVDMIYLKSLPPAGCSVLWDHLNCWPHAQLGETVSQPCPTFLRVKGDVMRNCTEAGWSEPFPPYELACGYTLNNTLHFPPDIMLMSHEYFMWVRIMYSVGYSISLISLIFALILLYMFRKLHCTRNFIHMQLFLSFILRAAFIFIRDAVLHNSQDYYHCDSHPPGCKVALLLSNYCILANYSWLLVEGHYLHSLIHLSLYSQRKRLHWYISLGWGTPVLIIIVWSVAKYLQENEGCWETRGNSWIWWILRVPVFLVIIVNLVFFMRIIRTLVRKMRAPDMIGNEFKQHRKLAKSTLLLVSLFGVQYALFTFFPDEVSILTFQIWNAIELAMASTQGFIVALLYCFLNGEVQHEIKRRWRRWQPKKRFHGDPRMHNGSLSHSGLPLTQISLLARANNMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGJ9</accession>
    <name>A0A3B4EGJ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Substance-K receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">NK-2 receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Neurokinin A receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Tachykinin receptor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TACR2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037394315.1">
        <property type="nucleotide sequence ID" value="XM_037538418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGJ9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034863"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027115.2">
        <property type="protein sequence ID" value="ENSPNAP00000034863.2"/>
        <property type="gene ID" value="ENSPNAG00000024465.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415119"/>
    <dbReference type="GeneTree" id="ENSGT00940000155512"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016497">
        <property type="term" value="F:substance K receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902093">
        <property type="term" value="P:positive regulation of flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000291">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001681">
        <property type="entry name" value="Neurokn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000913">
        <property type="entry name" value="NK2_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR TKR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925:SF3">
        <property type="entry name" value="SUBSTANCE-K RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01025">
        <property type="entry name" value="NEUROKININ2R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00244">
        <property type="entry name" value="NEUROKININR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 18" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 18" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 18 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="21">
        <location>
            <begin position="65"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="121"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601681-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034863.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="390" mass="45184" checksum="B29300DB6EC0B725" modified="2024-11-27" version="2">MSSKRCWDPPADMEATPEPLWSSTPALYDEDDGNDTSSNMFEQPDWQVALWAVAYSLVVLVSVVGNLTVIWIIVAHKRMRTVTNYFIVNLAFSDASMATFNTVFNFVYAVHNDWYFGLGYCKFQNFFPITAVFSSIYSMAAIAVDRYMAIIHPLKSRLSSTTTKVVIAVIWAVAFSLAFPQCFYSSTKFYSHRTVCMVEWPDDYGGKHQLSYQIAVIILIYLLPLLVMLVTYSLVGQRLWGSKIPGEASDHYQHQIQAKRKVVKMMIVVVMTFAICWLPYHIYFILGSFKRDIYNQHYIQQVYLAIFWLAMSSTMYNPIIYCCLNQRFRSGFRQAFRWCPLVKVSEEDNMELQRMRTFRTTRSYRTETTSMMSRNHANALEDATPKLIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJM2</accession>
    <name>A0A3B4EJM2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Netrin 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NTN5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJM2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035963"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039609.2">
        <property type="protein sequence ID" value="ENSPNAP00000035963.1"/>
        <property type="gene ID" value="ENSPNAG00000006479.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166316"/>
    <dbReference type="OMA" id="NGYTRNH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd03579">
        <property type="entry name" value="NTR_netrin-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000081">
        <property type="entry name" value="Netrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000048">
        <property type="entry name" value="Netrin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000217">
        <property type="entry name" value="Netrin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF383">
        <property type="entry name" value="NETRIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 8" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017477970" description="Netrin 5" evidence="10">
        <location>
            <begin position="29"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="13">
        <location>
            <begin position="23"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="11">
        <location>
            <begin position="534"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="12">
        <location>
            <begin position="604"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="274"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="274"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="534"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="536"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="567"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="785" mass="88764" checksum="B4BFE83D728A6B78" modified="2018-12-05" version="1">MSQYYSLPFPFLSLLFPLLLCFCPYSLLSPSLSQSSLRWTSPHDPCYHLDGRPRHCLSDFINAAYGVSVRAEDLLQGTERNITSLTDLHNPHNLTCWTASEDSAQGEWTFTVPLGRRFEVTYVSLQFCQLAEPTEPFSISILKSMDYGRSWRPLQFYSSDCKGKFSLPAQSVALTRHQETESLCSDPRPLQKHRGGVVLAFSTLDGRPSSADFEYSPGLQDWVTATDIRIVFHQSQGKTVQQGKKPGEVDGSLIWSQGSKSKIVTQAEKGRAENVLRVEKKDTMTKSTETKKRRKEGKTEKSLSRNHNKSSLQESGHNVTRKQSESQEGRGRGRGRKKDVHHWKPCQDGGCDWTVEGRGRGSKGRELRRRRNNAGSERSRSRFKNKATHHLSPSSLSAPSRLPLALSDIQVGGRCKCNGHASRCRRDQQGQAICQCEHHTAGPDCDVCESFYYDRPWQRATPSQAHPCVPCECNGHSTKCRFSMEVFQQSGRVSGGVCLKCRHHTAGRHCQFCQNGYMRDHTKSLSHRKACQPCQCHPLGAVGHWCNQATGQCLCREGVMGQRCNRCAPGYKQGRSPLRPCIRIPEVTPPTPVYQPQYSRGEECLSYCQPSQAKVRMNLETFCLKDYVLKVQIRGKERSGPWWQFSAWVQSVFRVGSSQVKRGLRAVWVPDRDLGCGCPALQVGRVFLLIGAEENRRSWAPGEKRLVADRSTMALQWREHWGPKLRGFRGQDNRGLHLVPLWCQTVRGKTCLCPSVKNLKAINFNTHIDTCNHRKTQRERERAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELZ3</accession>
    <name>A0A3B4ELZ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HSac2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TPRG1L</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPRG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561304.2">
        <property type="nucleotide sequence ID" value="XM_017705815.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELZ3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036289"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030625.2">
        <property type="protein sequence ID" value="ENSPNAP00000036289.1"/>
        <property type="gene ID" value="ENSPNAG00000005607.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432155"/>
    <dbReference type="GeneTree" id="ENSGT00390000001652"/>
    <dbReference type="OrthoDB" id="10012704at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034753">
        <property type="entry name" value="hSac2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022158">
        <property type="entry name" value="Inositol_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040242">
        <property type="entry name" value="TPRG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31108">
        <property type="entry name" value="TUMOR PROTEIN P63-REGULATED GENE 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31108:SF7">
        <property type="entry name" value="TUMOR PROTEIN P63-REGULATED GENE 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12456">
        <property type="entry name" value="hSac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51791">
        <property type="entry name" value="HSAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSac2" evidence="2">
        <location>
            <begin position="142"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="349" mass="39524" checksum="EF5959EE0B48AD70" modified="2018-12-05" version="1">MCVFKEMSIASEEWSRWVFLSLSKTSFGRLARRFPAGSGSVQLTRARLFECVLTLCYCRLLRFQPDTMLQLNDSMEPESPGAAVLHAAEVEDGVEVAFTPPAARHSRGFEAAAVACCSPLQKLTPFRVHNPTMQAKVKDYFVFRPGTIEQAVNDIRTVALPSEDGEVLSVWLLAEIDHWNNEKERLVLITERSLLVCKYDFINLQCQQVIRISLNAVDTISVGEFEFPPKSLNKREGTGVRVQWDKRPRPSFLNRWNPWSTDMPYATFTEHPMAHADEKVASLCQLENFKTQLIQAVKKAYKECPIPGRANGVLLLERPLLIETYLGIMSFINNEAKLGYSMTRGKIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ILX8</accession>
    <name>A0AAR2ILX8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">YWHAH</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552752.1">
        <property type="nucleotide sequence ID" value="XM_017697263.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ILX8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049749.1">
        <property type="protein sequence ID" value="ENSPNAP00000040688.1"/>
        <property type="gene ID" value="ENSPNAG00000030997.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108427260"/>
    <dbReference type="CTD" id="7533"/>
    <dbReference type="GeneTree" id="ENSGT01140000282547"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000001">
        <property type="entry name" value="14-3-3 gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="4"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="246" mass="27921" checksum="213F553C6242CE7D" modified="2024-11-27" version="1">MADREQLIQRARLAEQAERYDDMASAMKLVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVTSSIEQKTAADGSEKKLELVRAYRETIEKELESVCQDVLTLLDQYLIKNCDESQLESKVFYLKMKGDYYRYLAEVATGEKRASAVEASEAAYKEAFDISKSMAATHPIRLGLALNFSVFYYEIQNAPEQACQLAKEAFDEAIGHLDNLNEDSYKDSTLIMQLLRDNLTLWTSDQQDSEGGDPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IM90</accession>
    <name>A0AAR2IM90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017567230.1">
        <property type="nucleotide sequence ID" value="XM_017711741.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IM90"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044823.1">
        <property type="protein sequence ID" value="ENSPNAP00000040815.1"/>
        <property type="gene ID" value="ENSPNAG00000034781.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108435727"/>
    <dbReference type="CTD" id="556853"/>
    <dbReference type="GeneTree" id="ENSGT00940000159988"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099558">
        <property type="term" value="P:maintenance of synapse structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000002">
        <property type="entry name" value="Cerebellin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF50">
        <property type="entry name" value="CEREBELLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043490496" description="C1q domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="7">
        <location>
            <begin position="59"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="195" mass="21192" checksum="D7FA30D97B2B6593" modified="2024-11-27" version="1">MLRLLALALLLLLAAYGSGSVGLNDTEPVVLEGKCLVVCDSNPSGDGAGAASFGISVRAAGAKVAFSAVRGTNHEPSEMSNKSMTIYFDQVLVNIGNHFDLKASVFTAPRRGIYSFSFHVVKVYNRQTIQVNLMHNEYPIISAFAGDQDVTREAASNGVLLHLEREDRLYLKLERGNLMGGWKYSTFSGFLVFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IQI1</accession>
    <name>A0AAR2IQI1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IQI1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083261.1">
        <property type="protein sequence ID" value="ENSPNAP00000052543.1"/>
        <property type="gene ID" value="ENSPNAG00000033002.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000083529.1">
        <property type="protein sequence ID" value="ENSPNAP00000040302.1"/>
        <property type="gene ID" value="ENSPNAG00000031258.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="119" mass="12959" checksum="0DF380608E6E1D58" modified="2024-11-27" version="1">YTFGGGTKLLFGSLAAPTLTVLPPSSMELQQGKATLVCLANKGFPSDWRLSWKVDGSSWCSGISQSPGLLQKDDGLYSWSSTLTLQEDQWLKKTVTCEATKDSQPPVSQTLRREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IVB6</accession>
    <name>A0AAR2IVB6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">HNRNPH3</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IVB6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057106.1">
        <property type="protein sequence ID" value="ENSPNAP00000041977.1"/>
        <property type="gene ID" value="ENSPNAG00000031385.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000289222"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="454" mass="48377" checksum="1388ECCF62A7022F" modified="2024-11-27" version="1">MSEPHSGQIQAFHTRFGHLGSGFGRRGLGFGRSVSGLGWRGLGFGHLGSGYGRPGLGFGHLGSGFGRRGLGFGRSGSGLAWRGLGFGHLGSGSEGFGRLGSRLGRRGLGFGRLGSGLERRGLGFGRFGLGLGKRGSGFGRLGSGFRRQGLGFGYLVSGFRRRGLGFGRLGSGFRRRGLGFGRLGKRGSGFGRLGSGFGRRRSGFGRLGSGFGRRRLGFGRLGSRFGRRGSGFGRLGSGFGRRRLGFGRLGSGFRRRGLGFGCLGSGFRRRGLGFGRLGSGFRRRGLGFGRLGSGFRRRGLGFGRLGSGFRRRGFGRRSLGFGRLVSRFGRRRSGFGRLGSGFRRRGLGFGRLGSGFRRRGLGFGRLGSGFRRRGLGFGRLASGFRRRGLGFGRLGSGFRRRGLGFGRLVSGFRRRGLGFGRLGSGFRRRGLGFGRLGSGFRRRGLGFGRLGSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWG3</accession>
    <name>A0AAR2IWG3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWG3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065579.1">
        <property type="protein sequence ID" value="ENSPNAP00000042389.1"/>
        <property type="gene ID" value="ENSPNAG00000037384.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286952"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000839">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="140"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="187" mass="21670" checksum="FA393C51A411F603" modified="2024-11-27" version="1">MAFIRSFSTQQPFADPLLAAQTQTKPGKERTHRCFECGKSFTRQSTLQRHQLIHTGEKPFYCSQCGMSFNVQSNLQTHQRIHTGEKPYCCAECGKSFTRQNVLQRHQRTHTGEKPYHCSDFTRLSHLQRHQRIHTGEKPYYCSECGKSFRHSHTLKIHKCFEHPGSNDACKFTHGSLCRNVHFSVCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IXL5</accession>
    <name>A0AAR2IXL5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insulin-like growth factor-binding protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547081.1">
        <property type="nucleotide sequence ID" value="XM_017691592.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IXL5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053868.1">
        <property type="protein sequence ID" value="ENSPNAP00000042789.1"/>
        <property type="gene ID" value="ENSPNAG00000031524.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108423924"/>
    <dbReference type="CTD" id="403039"/>
    <dbReference type="GeneTree" id="ENSGT00940000155890"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031994">
        <property type="term" value="F:insulin-like growth factor I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031995">
        <property type="term" value="F:insulin-like growth factor II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043567">
        <property type="term" value="P:regulation of insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.20:FF:000001">
        <property type="entry name" value="Insulin-like growth factor binding protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000009">
        <property type="entry name" value="Insulin-like growth factor binding protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012213">
        <property type="entry name" value="IGFBP-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022321">
        <property type="entry name" value="IGFBP_1-6_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017891">
        <property type="entry name" value="Insulin_GF-bd_Cys-rich_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551:SF4">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01976">
        <property type="entry name" value="IGFBPFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01981">
        <property type="entry name" value="IGFBPFAMILY5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00222">
        <property type="entry name" value="IGFBP_N_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043916298" description="Insulin-like growth factor-binding protein 5" evidence="10">
        <location>
            <begin position="21"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="12">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="11">
        <location>
            <begin position="184"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="268" mass="29465" checksum="2B3BDD15D761CBA5" modified="2024-11-27" version="1">MLLGVCPLLPLVLAVARGLASYVPCEPCDQKALSMCPPVPIGCQLVKEPGCGCCLTCALAEGHACGVYTGTCAHGLRCLPRDGEEKPLHALLHGRGVCTNEKGYKAPHAPADRESKEHDDTMKTDATEEQLPQAKVPFNPKQDMINSKKQAAMRKEKKKQQEKLRSVGSPDYPALPIDKHEPEFGPCRRKLDGIIKGMKDTSRIMALSLYLPNCDRKGFFKRKQCKPSRGRKRGICWCVDKYGVQLPGTDYSGGNVQCKDLENSNNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J4Z0</accession>
    <name>A0AAR2J4Z0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Parvin, alpha b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the parvin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J4Z0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052624.1">
        <property type="protein sequence ID" value="ENSPNAP00000047078.1"/>
        <property type="gene ID" value="ENSPNAG00000003340.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183194"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071963">
        <property type="term" value="P:establishment or maintenance of cell polarity regulating cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21306">
        <property type="entry name" value="CH_PARVA_B_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21335">
        <property type="entry name" value="CH_PARVA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000015">
        <property type="entry name" value="Parvin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000011">
        <property type="entry name" value="Parvin, beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028433">
        <property type="entry name" value="Parvin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12114:SF6">
        <property type="entry name" value="ALPHA-PARVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12114">
        <property type="entry name" value="PARVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039131">
        <property type="entry name" value="Parvin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="118"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="10">
        <location>
            <begin position="285"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="13"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="395" mass="44681" checksum="A68D0A68A73BC58E" modified="2024-11-27" version="1">ILGIPLPFFFLTQPRREPLAAPTMASSPQKSPSSPKPPTPKSPPSRKKDDSFLGKLGGTLARRKKAKEVSELQEEGMNAINLPLSPTPFELDPEDTMLEENEVRTMVDPNSRNDPKLQELMKVLIDWINDVLVGERIIVKDLAEDLYDGQVLQKLFEKLEGEKLNVAEVTQSEIAQKQKLQTVLEKINDTLKVSSRNIKWNIDSVHAKSIVAILHLLVALSQHFRAPIRLPDHVSIQVVVVQKREGILQSRQVQEEITGNTEALSGRHERDAFDTLFDHAPDKLNVVKKTLITFVNKHLNKLNLEVSELDTQFADGVYLVLLMGLLEGYFVPLYSFFLTPENFEHKVHNVSFSFELMQDGGLEKPKPRPEGGCHFVPMITYICSYLSRKTHIQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J5F9</accession>
    <name>A0AAR2J5F9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J5F9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085548.1">
        <property type="protein sequence ID" value="ENSPNAP00000045474.1"/>
        <property type="gene ID" value="ENSPNAG00000035892.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165756"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21728" checksum="77497B9E42FCABC7" modified="2024-11-27" version="1">MAGIYGHMDVFDESGEQWATYIERFEHYVVANDIPESKKVSILFSVMGPKTYGLLRSLVAPVAPGTKTYEQVVTVLKDHFTPKPLVIAERFRFHKRNQGEGETVAQYVAVLKRLSEHCKFGAYLEDALRDRFVCGLMCEAVQKRLLTEDKLTFKKAVEHAVSAEIAARDVQQLSTPRAATHSLLPGRHTCQCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J8S6</accession>
    <name>A0AAR2J8S6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017549330.1">
        <property type="nucleotide sequence ID" value="XM_017693841.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J8S6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082565.1">
        <property type="protein sequence ID" value="ENSPNAP00000046251.1"/>
        <property type="gene ID" value="ENSPNAG00000033395.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108425274"/>
    <dbReference type="CTD" id="58061"/>
    <dbReference type="GeneTree" id="ENSGT00940000158311"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000166">
        <property type="entry name" value="homeobox protein Hox-C11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021918">
        <property type="entry name" value="DUF3528"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12045">
        <property type="entry name" value="DUF3528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="223"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="225"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="158"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="297" mass="33823" checksum="21FF3ED92BC8193F" modified="2024-11-27" version="1">MMDFDERVPVSSNMYLPSCTYYVPGADFPTLPSFLSQSPSSRPVPYSYSSNLPQVQPVREVTFRDYAAFDATGKWPHRAPLAQCYPGEDVVHRECLPASAGVGEMLAKNTSAVYHHHPHHHSTSGSSASSSTFYGSIGRNGVLPQAFDQFFDTAYGNLENAPSDEFTTAEKQQLQQQQQSSKQQTAAPAPDHDTEGKERGETSSPESSSGNNEEKRGEASNAPRVRKKRCPYTKYQIRELEREFFFSVYINKEKRLQLSRMLNLTDRQVKIWFQNRRMKEKKLNRDRLQYYTTNPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JGQ9</accession>
    <name>A0AAR2JGQ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JGQ9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046990.1">
        <property type="protein sequence ID" value="ENSPNAP00000049036.1"/>
        <property type="gene ID" value="ENSPNAG00000007806.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000209990"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00436">
        <property type="entry name" value="INTERLEUKIN8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043344464" description="Chemokine interleukin-8-like domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="3">
        <location>
            <begin position="36"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="127" mass="14863" checksum="BFBAA1C2EB4C8310" modified="2024-11-27" version="1">MSCCLRLCPQCICIPLLMSKTLSLSTFQDSQHVPTRCECQKSMRAVRGPFSDIKVSLKGHGCDKDEIIVTLKSNNKQMCLSPKERQGKRLLKCWLKMQKKGEDVKKCIQRLRPQKRRRQKKPWRNRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JIS1</accession>
    <name>A0AAR2JIS1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570479.1">
        <property type="nucleotide sequence ID" value="XM_017714990.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JIS1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081735.1">
        <property type="protein sequence ID" value="ENSPNAP00000050207.1"/>
        <property type="gene ID" value="ENSPNAG00000029265.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437709"/>
    <dbReference type="GeneTree" id="ENSGT00950000182857"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051517">
        <property type="entry name" value="IFITM_antiviral_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13999:SF31">
        <property type="entry name" value="IFITM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13999">
        <property type="entry name" value="INTERFERON INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="18318" checksum="F0052DF9A693AC80" modified="2024-11-27" version="1">MSQDRFPESVWVWICCNLRSCNKGRQIQDIQQHRTRTATEMQSSYRPMENLPLQGVTGDGTVVVPMPEHPKDYIVWSMSTLYFGNPFCLGLLAFYFSVKSRDRKMVGDLSGAKSYGSTACCINGWALGLIILFIIAAIVIIAATVPAVVNRIQSMSSSDSYSYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JMA2</accession>
    <name>A0AAR2JMA2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">COG1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570978.1">
        <property type="nucleotide sequence ID" value="XM_017715489.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JMA2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050949.1">
        <property type="protein sequence ID" value="ENSPNAP00000053145.1"/>
        <property type="gene ID" value="ENSPNAG00000002435.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438011"/>
    <dbReference type="CTD" id="9382"/>
    <dbReference type="GeneTree" id="ENSGT00390000017136"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033370">
        <property type="entry name" value="COG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31658">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31658:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="983" mass="108358" checksum="2F0548472AC12138" modified="2024-11-27" version="1">MASVPAHSLRVSDIKDPNVLFERYSTEEIRAVERKIRGEIEQKKEELRQMVGERYRDLIDAADTIGEMRQCSESVVRSIQDMDRYCQQLKQSRNPLQSLSKLEDQKQSKEKFYSIASQIKLLLEIPERIWSAMECSQYLQATQLYLLCCHLHRQLHLDGGSPHYSPVLSRFPILVRQVAAAGHFRSTILLDSKSVLRGRAVSDQAIAEALVSTMLLEGSSPRQALADFLLARKASIQQLLNQPQHGAGIKAQVCSLVELLVTTLYQAYAVFYLPPEGVRGVDPGLSCGLLFTTLENVTSTTSTTKGRKVLHGEGSAGSWFKYLPPSVTDFQPVLRTLAQPIQSDQLRDTLQQWIDTCKEDIRGGVSSLLVYVKSLKALAAIRDAVWELLSSESISLHWSAVCLRLLEKPLCLWDDLLQQLFLQRLQAITQEGMEGISSSSRQLLSSALRDLEAQSQSGVFGRGAQYESDVAAFLWTESQGDLPSDAAWVSVSQRGPQLYPQVKSSLSMKTQALTPCIQSFCSALDTKLKVKLEDLQHYLPPEPAVKDQPESTPAPATPSFNRFTDSSAVEEALREHCLACVRDILSSVRSQLTGLQADPVGSGRMSSTLFMARLCQSLGELCPSLRQCVLGQQGGAVDAAGRGTPRQGKKLGKGGAVKGVELSPAQAKWAGLKEELLACSMEAYRIWSSAVSKALLASFTGSLHAGSAGSILATATHWEEQEIQEEAESGNTIMSKIHLPVQPSWYVQSLLFQLCLEVNRVGGHALPKVTLLELLRGCMDQVLTEYEKLTQRSERKDAAPPITQNRALQLLFDLRFLSATLGSRLEEGKSSRSQQDPRIQEICDLLEGHIDPFDLDVFTPHLNSNLNRLTQRTAVLLGLLTGTEKQFVPRSSSVGSQEPYNILPLASSQIRFGLLPLSMTNSRKAKSTSRATDITKPLMAPSSTPAPEDTFRPGNLFRQLANQDEEPAGPSLFKLGWLSGMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JNI6</accession>
    <name>A0AAR2JNI6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">General vesicular transport factor p115</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein USO1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transcytosis-associated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Vesicle-docking protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">USO1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VDP/USO1/EDE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037394595.1">
        <property type="nucleotide sequence ID" value="XM_037538698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JNI6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050656.1">
        <property type="protein sequence ID" value="ENSPNAP00000053575.1"/>
        <property type="gene ID" value="ENSPNAG00000004919.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439957"/>
    <dbReference type="GeneTree" id="ENSGT00390000017018"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:transcytosis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="General vesicular transport factor p115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
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    <dbReference type="InterPro" id="IPR041209">
        <property type="entry name" value="P115_Arm_rpt"/>
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        <property type="entry name" value="Uso1_p115_C"/>
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    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18770">
        <property type="entry name" value="Arm_vescicular"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uso1_p115_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uso1_p115_head"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="ARM" evidence="19">
        <location>
            <begin position="60"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Vesicle tethering protein Uso1/P115-like head" evidence="22">
        <location>
            <begin position="363"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Uso1/p115-like vesicle tethering protein C-terminal" evidence="23">
        <location>
            <begin position="848"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="938"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="674"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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            <dbReference type="ARBA" id="ARBA00006960"/>
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            <dbReference type="ARBA" id="ARBA00018243"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022892"/>
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            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023034"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF04869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF04871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="964" mass="107763" checksum="CE02095D6CD54972" modified="2024-11-27" version="1">MNFFRGVIGGQQAGAQPSGVETIQKLCDRVASSTLLEDRRDAVRALKSLSKKYRMEVGTQAMDHLIRILQTDSSDSEILGYALDTLYNIICSDEEEEQDESEDAAPVKSKNVAAPEDTQRRQEDDIGAQFSERFIQEPENVTLLLTMLEEFDFHVRWPGVKLLTALLKCQCAQVQGIILVSPMGVSRLMDLLADSREVIRNDGLLLLQQLTKGNAAIQKIVAFENAFERLLDIITEEGTSDGGIVVEDCLLLLLNLLKNNSSNQNFFKEGSYIQRMKPWFEVGDDNSGWSAQKVTNLHLMLQLVRVMVSPVNSPGATASCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGSQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVATLLPSTIDANSISAGQLLCGGLFSADSLSNWCAAVALGHALQDNLTQKEQLLRVQLATSLGKPPVSLLQQCTNILSQGDKISRRGSKVQTRVGLLMLLCTWMSNCPIAVTHFLHNQDNVPFLTAQISENLGEDERLVQGLCALLLGICIYYNDNSLENYTKEKLKQLIEKRIGKENFVEKLGFITKHELYSRAAQKPQPAFSAPEHMLFDHEFTKLVKELEGVITKAVHKSSEEEKKEEEVKKTLEQHDSIVTQYKELIREQDAQINELKEQMCTLTSQKDQMQNTITQQLSQIQQHKDQYNVLKLKLGKDSGQSDSGQVNGLQLEELTLLREELEELRKQHALLNTQLAERDTLIANLKAEGVQSPDGLTAAPENTELLKELESLRGQVQTQAAEISQLQRERQEVQTRTEALAAVSGEAADLTKVTELENRLSAQTAETNRLQEEVRSLTESRAAMEKERAAATSTATILQTEKSKLQQEVAESKKEQDDLLMLLADQDQKILSLKQRLKDLGETIEDEDDLDARDQFDDDDDEEEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JTY1</accession>
    <name>A0AAR2JTY1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="12">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NME6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="12"/>
    <dbReference type="RefSeq" id="XP_017565986.1">
        <property type="nucleotide sequence ID" value="XM_017710497.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JTY1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060203.1">
        <property type="protein sequence ID" value="ENSPNAP00000055503.1"/>
        <property type="gene ID" value="ENSPNAG00000030182.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108434999"/>
    <dbReference type="CTD" id="10201"/>
    <dbReference type="GeneTree" id="ENSGT00940000160284"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045839">
        <property type="term" value="P:negative regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04414">
        <property type="entry name" value="NDPk6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000006">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037994">
        <property type="entry name" value="NDPk6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46956">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46956:SF1">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="13">
        <location>
            <begin position="12"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="10">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="182" mass="21560" checksum="F377705475BD02F1" modified="2024-11-27" version="1">MILSTRRCARVLQLTLAVIKPDAVAHPLILEALHQKILENNFVIVRKKDLIWRRSDSERFYAEHEGRFFYQRLVEFMASGPMRAYVLAREDAITRWRELMGPTKVYRARYTSPSTIRAQYGLTDTRNTTHGSDSEESAQREIAFFFPEFSIDQWLEKEEPCLRVGHMDYDQQRQIHNPFKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JUD2</accession>
    <name>A0AAR2JUD2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JUD2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070540.1">
        <property type="protein sequence ID" value="ENSPNAP00000055605.1"/>
        <property type="gene ID" value="ENSPNAG00000036132.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183155"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052749">
        <property type="entry name" value="Alpha-tectorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160">
        <property type="entry name" value="ALPHA-TECTORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160:SF9">
        <property type="entry name" value="PROTEIN PRY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="7">
        <location>
            <begin position="176"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="6">
        <location>
            <begin position="429"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="73872" checksum="77429AF85F633139" modified="2024-11-27" version="1">MLFFASKIVLLCKNGEISLIVVSAEKKPKETIFYICFYIAFCGKFTTVNWSFSFPFKEYSCPSNSHYELCGTSCPAACPSLSFPFLCTQPCQEGCQCNDGLLLSGDSCVPPTGCGCLHEGRYRQGGESFWYGEGCQSLCICNGTTGFVRCSTSSCSEQETCRIVEGEYGCHPRPHATCSASGDPHYTSFDGRTFDFQGTCRYVLTTVCNRTRGLPYFQVIARNEAWQGLPVSITVEVYVNVSGHMIDSQTRNLPVLLDSGRVSIYSSGQNTFITTDFGLIVSYDGSWVVRVTVPANYSGATCGLCGNFNGQRGDDFRKRSENDTLCSDGCGDSCSSCQNPVEPRAQCQILRDSQGPLSFCHATVDPQAYFDDCVFDLCISENRHDVLCRSIQTYVSACQTANVVIYPWRQNTTCSEYFKYNRHLNSTETCQSSSATMSLSRCQLFEAGFPANVLHLSDPSCRGTVENGRLVFHFDNDDHICGTNLTVLNNSKRCRGIDLILFFCILLCYALTSLFFFFKHSIVSKILPDGQGMYQVRMIPYEDAAFSHPYNGTVDIVMDQKIYVEVNVQGVDSRQISTVIDSCWATPVNDRNYAVRWDLIINKCPNPNDISMEVLQNGVSTTARFSLKMFAFRRENSRVYLHCAIHLCLLRDNNCAVVSVLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JXR3</accession>
    <name>A0AAR2JXR3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MYH8</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000050447.1">
        <property type="protein sequence ID" value="ENSPNAP00000055037.1"/>
        <property type="gene ID" value="ENSPNAG00000034166.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162888"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000004">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000006">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000013">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000007">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF44">
        <property type="entry name" value="MYOSIN HEAVY CHAIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9 13" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="17">
        <location>
            <begin position="36"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="16">
        <location>
            <begin position="89"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="13">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1903"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="843"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1903"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="182"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1935" mass="222142" checksum="19A2A1ADADCFD9B1" modified="2024-11-27" version="1">MSTIMGDKDMECFGAAAIYLRKPERERIEAQNKPFDAKTAYFVAEPKEMYLKGTLKSKEGGKVTVETLCGKTITVKEDDIFPMNPPKFDKIEDMAMMTHLNEPTVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYDPVVVVGYRGKKRVEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVSGQKKAETVSGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGSSGKLASADVETYLLEKSRVTFQLSAERSYHIFYQLMTGHKPELLETLLITTNPYDYPLISQGEIVVKSINDVEEFIATDTAINILGFTADEKINIYKLTGAVMHHGNMKFKQKQREEQAEPDGTEVADKIAYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVNNAVMALCKSIYEKMFLWMVVRINEMLDTKQPRQFFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIDWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLHDQHLGKSKAFEKPKPTKGKAEAHFSLVHYAGTVDYNIVGWLDKNKDPLNDSVVQLYQKAANKLLSFLYASKTSSAGKKGGKKKGGSFQTVSALFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPDGQFIDNKKAAEKLLGSIDVDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLANLVTMTQAVCRGHLMRREFIKMMERRESILTIQYNIRSFMNVKHWPWMKLYFKIKPLLKSAETEKELAELKDNYEKMKENLAQALAKKRELEEKMVTLLQDKNDLQLQVASEVENLSDAEEKCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMASQDESIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERDRRKLEGDLKLAQENIMDLENDMQQSEEKLKKKDFETSQLLSKIEDEQSLSAQLQKKIKELLARIEELEEEIEADRAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEEATLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLCSNMEAVAKSKANLEKMCRTLEDRLSELKTQYDENVRQLTDLSTQKSRLATEAGELGRQLEEKETLVSQLTRGKLAYTQQIEELKRHIEEEMKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEETTVAINSKCTSLEKTKQRLQGEVEDLMTDIERANALAANLDKKQRNFDKLLGEWKQKYEESQAELEGSQKEARCLSTELFKMSNSYEETLEQLETLKRENKNLQQEICDLTEQLGESGKTIHELEKAKKTVESEKSEIQTALEEAEGTLEHEESKILRVQLEFNQLKSEIDRKLAEKDEEMEQIKRNSQRVIESMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKHLRNVQGQLKDAQLQLDEVVRGQEDMKEQVAMVERRNNLMLAEIEELRAALEQTERGRKVAEQELVDASERVALLHSQNTSLLNTKKKMEADLIQIQNEVDDTIQDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDETESLALKGGKKQLQKLEARVHELEAEVEAEQRRGVEAIKGVRKYERRMKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQAEEAEEQANTHLSRFRKVQHELEEAQERADIAESQVSKLRAKSRDAGLVGSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1X7</accession>
    <name>A0AAR2K1X7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Syntaxin-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1X7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045472.1">
        <property type="protein sequence ID" value="ENSPNAP00000056444.1"/>
        <property type="gene ID" value="ENSPNAG00000030390.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13786" checksum="80EB1FE5C1D280C6" modified="2024-11-27" version="1">MHLRTRVCVRARVCICVRGCARVCICVRGCARVCICVRGCARVCICVRGCGCVCICVRVWEWVCMYLHTCVGVGVYVFAYVCGSGCVCICIRVWECVCVCICIRVWECVCVCICIRVWECVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KB13</accession>
    <name>A0AAR2KB13_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar fusion protein MON1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an important role in membrane trafficking through the secretory apparatus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the MON1/SAND family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580657.1">
        <property type="nucleotide sequence ID" value="XM_017725168.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KB13"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056977.1">
        <property type="protein sequence ID" value="ENSPNAP00000059674.1"/>
        <property type="gene ID" value="ENSPNAG00000018371.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444170"/>
    <dbReference type="CTD" id="325065"/>
    <dbReference type="GeneTree" id="ENSGT00390000006665"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF13">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="4">
        <location>
            <begin position="182"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="5">
        <location>
            <begin position="343"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="6">
        <location>
            <begin position="473"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="606" mass="67820" checksum="D9C9EA7A6FE01EC3" modified="2024-11-27" version="1">MHNMDKGDQERQTMDEKLDSFISTQPAQTDLQVSSDVIPSENDADPIVEDIFPDEGGDRTVQNLNGHTEVHLGDITSEDATQLQAVPLDIQKEEITDFDKSVSAGMSETDSGKVSEDSALVDNGHDDSGEFVVAMLARGKLEEQGMGLKGTSSPLSETGTALGASSHRDEDVTTENWRQHRKHVFVLSEAGKPIYSRYGSEEALSSTMGVMMALVSFVQSGDNVIRSVYSDEHTVVFMQQGPLVLVSVSSSRQSEQQLRDELLYVYNQIVSMLTQASITRIFEHKKNYDLRRLLAGSEKILDGLLNLVDSDPSFLLSAIHCLPISSSFRDSLSQILQKAITPNLVFSILIAKNQLLTIVQEKMVIEDARLDPADLHLLLNLIGASSAFQAGEIWTPICLPSFNPDCYFYAYISYLDPPDCTVCLLLLSTDKEAFYAVAECKRKIEEAMLAQNALSSIAKAQSYSVSQVGVSDLRHFMYKPFDVPDNHRQLTQFTSPEMEAPYSSDEEKLRLLDLYRDLHSRIHSTSRPLKLIYHVAERETLLAWVTSKFELYTCFSPLVTKACAINAITKLLRWVKKEEDRLFIRYPPKYSTTPNPKSSRDTQRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KBX5</accession>
    <name>A0AAR2KBX5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FAAP100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KBX5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068590.1">
        <property type="protein sequence ID" value="ENSPNAP00000059596.1"/>
        <property type="gene ID" value="ENSPNAG00000036792.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000284491"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45598:SF1">
        <property type="entry name" value="4FE-4S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45598">
        <property type="entry name" value="PROTEIN CBG11839-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="568"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="609" mass="61241" checksum="008C1B8CA63FD659" modified="2024-11-27" version="1">TANLISCPHSSGSTASLSLSLSVCVSLSLCLCVSLSLSLSLSLSLSVCVSLSLCLCLSLSLSLSLSLSVCVSLSLCLCVSLSLSVCVSLSLSLSLSLSVCLSLSLSVCLSLSLSVCVSLSLCLCVSLSLSLCLCVSLSLSVCVCVSLSLSLCLCVCLSLSLSVCVSLSLSVCVCVSLSLSLSVCVSLSLSLCVCVCLSLSLCVSLSLSVCVSLSLCVCVCLSLSLCVCVSLSLCLCVCVSLCLCVCVSLSLSVHLSLSVCVSLSVCVCLSLSVCVCLSLSLSLSVCVSLSLSLSVCVCLSLSLSVCLSLSLCLCVCLSLSLCLCVCLSLSLSVCVCLSLSLSLSVCVSLSLSLSLSVCVSLSLSVCLSLSLSLSVCVSLSLSVCVSLSLSLSVCVSLSLCLCVCLSLSLSLSVCVCVSLSLCLCVCVSLSLCASLSLCVSLSLSASLSLRLALSLSLSLSLCVSLSASLSLSLCVSLSLCVSRSLSLCVSLSLSASLSLCVSLSLSLRLSLSLSASLSLSASLSLSLHLSLSLSASLSLSLSLCVSLSLSLCLALSVCLALSLSASLSLSLCVSLSLCVSLSLCVSLSLSLALSRYSKCDTSPHQTIHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KF31</accession>
    <name>A0AAR2KF31_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FERMT1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KF31"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050715.1">
        <property type="protein sequence ID" value="ENSPNAP00000060716.1"/>
        <property type="gene ID" value="ENSPNAG00000019340.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013444"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13205">
        <property type="entry name" value="FERM_C_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000037">
        <property type="entry name" value="Fermitin family homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF12">
        <property type="entry name" value="FERMITIN FAMILY HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18124">
        <property type="entry name" value="Kindlin_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043613745" description="PH domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="383"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="695" mass="80009" checksum="880E76A44CFCC93A" modified="2024-11-27" version="1">MFALHSFQLPLALCHCSSTIVLFVTTRSEVHLMSQPERTAHTHTHTFAHTHGLRGVRTAQRCKNQFPNITQKLKLKLLFAVTEAPQNWSDHALWWEQKNCWLLKNHWTLDKYGVQADADLRFTPQHKPLCLQLPNMKTIKLTITFSSVVFKAVEEICKALNIRRSEELSLLRPPDDPKKKKKKDKGKGQAEDDIIDMDTISGVAGNSLYSKTMTATYDPENGTPASATSLWFGDNPLTASQPNIPAEELAKMYLPLSMEDKAVINAGWLDSSRSLMEQGVQENDKLLLRFKYHCFFDLNPKYDAVRITQLYEQARWAILLEEIECTEEEMLMFASLQYHICKLTMSTKPVDLSDEPEIDEVEAALSHLETTLEGRSTDRILVCISTQPYLFVPSVLCRPKRLLRTFKEYWFVFRDTSISYYKNKESANGEPIEQLHLRGCEVVPDVNVTEKKFGIKLLLPVADGMNEVYIRCDHETQYAKWKAACILASKGKTMAYSSYNTEVKNIQSFLQMKSLAPPPGQAAPDVESMEMNAECFVSPRYAKRYKNKQLSTRILEAHHNISQLTLMEAKLRFIQAWQSLPEFGIKYYIVRFKGCKKDEILGISYNRLIRIDMSTRLPLTTWRFSNMKQWNVNWEIRQVTIEFDQNVSVAFSCQSCDCRVVHEYIGGYIFLSTRSKDQNETLDEELFRKLTGGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJL3</accession>
    <name>A0AAR2KJL3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vitellogenin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000065580.1">
        <property type="protein sequence ID" value="ENSPNAP00000064470.1"/>
        <property type="gene ID" value="ENSPNAG00000033813.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064273"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.20:FF:000002">
        <property type="entry name" value="Vitellogenin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.80.10:FF:000001">
        <property type="entry name" value="Vitellogenin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.230.10:FF:000002">
        <property type="entry name" value="Vitellogenin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.80.10">
        <property type="entry name" value="Lipovitellin-phosvitin complex, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.50.20">
        <property type="entry name" value="Lipovitellin. Chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.90.10">
        <property type="entry name" value="Vitellinogen, beta-sheet shell domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015258">
        <property type="entry name" value="Vitellinogen_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037088">
        <property type="entry name" value="Vitellinogen_b-sht_shell_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015817">
        <property type="entry name" value="Vitellinogen_open_b-sht_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050733">
        <property type="entry name" value="Vitellogenin/Apolipophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345:SF9">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09175">
        <property type="entry name" value="Vit_b-sht_shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01170">
        <property type="entry name" value="DUF1944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043994984" description="Vitellogenin domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="domain" description="Vitellogenin" evidence="9">
        <location>
            <begin position="17"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1044"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1045"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1319" mass="142812" checksum="E1DFFE03997CB772" modified="2024-11-27" version="1">MRAIVLALTVAFVVPDFGAGKTYVYNYEGQLLGGLPQEGLGKAGIKVSSKVLLRAEAQNTFVLKLADPQIYEYAGIWPQDRFAPAAKLTEALTADLATPIKFEYANGAVGKVFAPARVSAAALNVHRGILTILQLNLKNTQNVYEMHEAGPHGVCKTHYMITEDEKTHEITVLKSKDLTNCHERVIKDTGFAHAEKCVECQQRLQSLTGTATYNYTMKPTDAGVQLSEATVDEIHQYSLFNTRTGAVQMRVRQTLQLMEVQNTPIAPDTAQYSARGSLKYEFGAELLQTPIQLLEIKNAVAQTAEVLQHLAANNVDMVHEDAPLKFLELIQLLRAATFENIEAIWNQHKTKPLHRQWILDAAPLAGTPAALRFINEKFQADEFSAPEFTRALLLGLHMATADPEAIQQAASLAQSPKISAMPALREVIMLGYGSMVAKYCAEVHTCSADHLKPVHDNAAEAIAKGEISEISLALKVLGNAGHPASLKPIMKLLPGFGSAAAAMPTRVHVDAILALRNIAKKEPKLVQPVALQLFMDKNLHPEARMVACIVLFETKPSVALMSTVAGEIEKEPRNEVASFAYSYIKSLTRSTAPDMVKVAAAANVAVRILSHKLDRQSYFSSTAYHSDLYVSPLMAGAAGTAFIINDAATIVPRAVVAKARAYLAGAASDVVEVGVRTEGLQEALQRTPAADKNADRITKIKHTLQALMNWKAMPASEPLGSIYFKAFGQEIAFTNIDKTFIDNAIEQAAKPRAHQLLKETVKALQAGISYQFAKPMLGTEVRRIIPTAVGVPMELGIYSAAVVAAAVNVKATVTPPLPENLETGILDQLRKTDIELQAEARPGVATQTLAVIGVNWPLIQAVVMARGKIHTVAPFKVAARADIPRGNIKVQILPAAAPDHVAAVSFDTVAVVRNLEDHPADREISLAPAAPSGAQSLIPASIQKSLCGKVPYFQVKGCIEVASRNAGFMGYNPIYYVVGQHSARISVERGDGPALERVEFEVYIAPEKAPEKSVRETNPALGETQEENIILLKLREYLEAGLKKSSSSSSSSNSTSSSRSSSRSSSRSSIIRFLFLLQYLATQITSKASSSSSIEALQRQAKLLEDSIVPAFVIIARAVRSDRKLGYQLAAYLDKPNLRAQLVLSSISNDQWKLCADGVLSSKHKASARVGWGSECQDSSATVKAETGLHESSPAARFQLDWDKAPRLVTTAAMNAIRICEYLPTQIAAHLAGIAVNRAENKANSVGIIIALPTKRSADVIVKTPRMTAAKMAMPLPFALPVEKDGSIPALQNLDIQDAIQKLDIRHSKTEYCVFIVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJM9</accession>
    <name>A0AAR2KJM9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KJM9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072727.1">
        <property type="protein sequence ID" value="ENSPNAP00000064523.1"/>
        <property type="gene ID" value="ENSPNAG00000035040.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF966">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="43"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="320" mass="36320" checksum="B763071C59E25703" modified="2024-11-27" version="1">MVPFENTTFVRPPTFYINGLYNMPHSKYYYMFLCFVYAVTVLGNSFIMGTICLARTLHTAKYIAVFNLALSDLFGSSALIPKYADMFLLENQYISYEDCMTNLFFVFVFMIMQSFTLLALAFDRVIAICFPLRYHAIVTKTTMSLIIGVMWLVSVAIMVVTLALLTQLSFCRSTTVGSYFCDNGPIFRLACNDNSPNFIMAYVYVAIVLCLPLTLIAISYVCIGFALLKISQGADRIKAMKTCTSHIILVAIFYLPILSVYISALTTYIHPNTRIMNSVLTQTIPPMLNPIIYSLKTEEVLQSIKVLYKRIKTLLRYKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKI1</accession>
    <name>A0AAR2KKI1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKI1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063348.1">
        <property type="protein sequence ID" value="ENSPNAP00000062927.1"/>
        <property type="gene ID" value="ENSPNAG00000016593.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234648"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06376">
        <property type="entry name" value="PBP1_mGluR_groupIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000196">
        <property type="entry name" value="Glutamate metabotropic receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutamate receptor, metabotropic 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001786">
        <property type="entry name" value="GPCR_3_mGluR4"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01054">
        <property type="entry name" value="MTABOTROPC4R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="572"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="678"/>
            <end position="705"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="736"/>
            <end position="757"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="769"/>
            <end position="791"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="811"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="572"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50259"/>
        </source>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="933" mass="103961" checksum="BD814B109F3A1859" modified="2024-11-27" version="1">YCVKGGLQWIWSHLLSNNNFKPCYAHSIRIDGDIILGGLFPVHARGERGAPCGELKKEKGIHRLEAMLFAIDLINKDPDLLPNVTLGARILDTCSRDTYALEQSLTFVQALIERDPSDVRCASGEPPIFAKPDKIVGVIGASASSVSIMVANILRLFKIPQISYASTAPELSDNTRYDFFSRVVPPDSYQAQAMLDIVTAMGWNYVSTLASEGNYGESGVEAFVQISRETGGVCIAQSLKVPREPRAGEFDKIILRLLETPNARAIIMFANEDDIRRILNAAKRNNLTDHFLWVGSDSWGSKISPVVQQEKVAEGAITILPKRASVDAFDRYFRSRSLSNNRRNVWFAEFWEENFGCKLGMHGKRPGSPKKCTGLEKVGRDSSYEQEGKVQFVMDAVYAMAHALHRMHRDLCSGYPGLCPRMSNIDGKELLGYIRNVSFNGSAKTPVTFNENGDAPGRYDIFQYQITNGSTAEYRVIGHWTDHLHLNMDAMQWVSGDSSVPASVCSLPCQTGERKKVVKGVPCCWHCERCEGYHYQASEFTCQLCPYEQRPDHNRTGCQPIPIIKLEWHSPWAVFPVFIAILGIIATTFVIVTFIRYNDTPIVRASGREMSYVLLTGIFLCYIITFPMIATPGVAVCSFRRIFLGLGMCFSYAALLTKTNRIHRIFEQGKRSVAAPRFISPASQLVITFSLISVQLLGVLVWFIADPPHTIVDYGEQRTQDPENARGVLKCDISDLSLICSLGYSILLMVTCTVYAIKTRGVPETFNEAKPIGFTMYTTCIIWLAFIPIFFGTAQSAERMYIQTTTLTVSLSLSASVSLGMLYMPKVYIIIFHPEQNVPKRKRSFKAIVTAATMSSKLSQLASESAVSLILNTLNVLGIIRFVCSGKESEAHTFNVRKSYQILVHLVCNYIISPERSPDPQHVNSVALNSVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KM81</accession>
    <name>A0AAR2KM81_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KM81"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076562.1">
        <property type="protein sequence ID" value="ENSPNAP00000065438.1"/>
        <property type="gene ID" value="ENSPNAG00000035537.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282498"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
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    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18720" checksum="7560A676AA83EE2B" modified="2024-11-27" version="1">MRLDVRILQCQLKTYRNLWHIKSLNKNDVHGRTPQRKPPLSTQNNAAHLKFAKEHLDVPQHYWQNILWTDETKIELIGRNTQRYVWRKKGTAHQHQNLIPTVKYGGGGIIFWGCFASSGPGRIAVIPSLSRPFAGKLKTVNRTTTQNIEVNHQQNGCGTETFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KXG5</accession>
    <name>A0AAR2KXG5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">COPS3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KXG5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080808.1">
        <property type="protein sequence ID" value="ENSPNAP00000067072.1"/>
        <property type="gene ID" value="ENSPNAG00000020244.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153653"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000354">
        <property type="entry name" value="COP9 signalosome complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000008">
        <property type="entry name" value="COP9 signalosome complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055089">
        <property type="entry name" value="COP9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048621">
        <property type="entry name" value="CSN3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF1">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22788">
        <property type="entry name" value="COP9_hel_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21215">
        <property type="entry name" value="CSN3-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="192"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="425" mass="48392" checksum="F09FB2951B19F94A" modified="2024-11-27" version="1">MLGTGRGDLRIMSSQMTQLCELINKSGELLAKNLSHLDTVLGALDIQEHSLGVLAVLFVKFSMPNIPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGISILKQAIEKMQMNTNQLTSIHADLCQLCLLAKCFKPAVPFLELDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFFEQAITTPAMAVSHIMLEAYKKYILVSLILHGKVQQLPKYTSQIVGRFIKPLSNVYHELAQVYTTNNPAELRSLVNKHSETFTRDNNTGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIYASINQKDGMVCFHDNPEKYNNPAMLHKIDQEMLKCIELDEKLKAMDQEITVNPQFVQKVRRFLTVLNILVISFREASSDPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L058</accession>
    <name>A0AAR2L058_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SLC4A7</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L058"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046396.1">
        <property type="protein sequence ID" value="ENSPNAP00000068007.1"/>
        <property type="gene ID" value="ENSPNAG00000023141.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157045"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF105">
        <property type="entry name" value="SODIUM BICARBONATE COTRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="604"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="634"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="677"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="815"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="853"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="901"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="941"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1001"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1027"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1070"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1097"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="19">
        <location>
            <begin position="127"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="19">
        <location>
            <begin position="387"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="18">
        <location>
            <begin position="571"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="524"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="1134"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1242" mass="137947" checksum="52D14A569B2F12B0" modified="2024-11-27" version="1">MEAAAASATAILTDMDERHEEGEHVLRSHGEEAVVDHGKTSSATHTNFEKEELESHRAVYVGVHVPLGKESRRRHRHRGHRHHRKRNRERDSDQEAGERESPTYDTPSQRVQCILGTEDGDVEHVPHELFTELDELSARHGNTEWRETARWLKFEEDVEDGGERWSKPYVATLSLHSLFELRSCILNGTVLLDMRAGSIEDIADNVIDSMVASGQLDESLRLKVREAVLKRHHHQNERKLSNRFPLAVRSLADIGKKHSDPLLLERSGDGLSASRNSLLKLGGSISNLSQRRESRVSMLLNHLLPGTAHGPSPSPSPQSTPPPSRRFANRPPVHLDIPAVVVSPPVEDEEAEGISAEEDALDFTACEGPLVSATSLSGSLDAGRPSENRSSKSNSKDSSTVDLSKVDMNFMKKIPPGAEASNVLVGEVDFLDRPIIAFVRLSPAVLLTGLTEVPVPTRFLFLLLGPYGKGPQYHEIGRSMATLMTDEIFHDVAYKARDRVDLLSGIDEFLDQVTVLPPGEWDPTIRIEPPKNVPSQMKRKRPSQANGMATPAEPPQEEEHHIGPELQRTGRVFGGLVRDVKRKAPFYWSDVVDALSLQCVASVLFLYCACMSPVITFGGLLGEATKSNISAIESLFGASLTGVAFSLFAGQPLTILGSTGPVLVFEKILFKFCEDYGMVYLSLRCSIGLWTAFLCVVLVATDASSLVCYITRFTEEAFAALICIIFIYEALEKLLHLGENYPVNTHNDLDNLTLYSCQCSAPDQVSNITLQLWNNTGHTGDSIPWNQLNVSMCKLLHGEFVGSACGHHGPFIPDVLFWSIILFFTTFFLSSFLKQFKTERYFPTKVRSTISDFAVFLTIMVMVLVDYLMGIPSPKLNVPDRFEPTSKHRGWLISPLGVNPWWTLLGSVIPALLCTILIFMDQQITAVIINRKEHKLKKGCGYHLDLLVVAVMLGVCSVMGLPWFVAATVLSISHVNSLKLESSCSAPGEQPKFLGIREQRLTGVLIFTLMGCSVFMTSALKFIPMPVLYGVFLYMGVSSLKGIQFFDRIKLFGMPAKHQPDLIYLRYVPLWKVHVFTLVQLTCLVLLWVIKVSSARVVFPMMVLALVFIRKLLDFFFTKRELSWLDDLMPESKKKKEDDKKKKAKQEAEECLEEEDEARLSYAGGGGGGGGNVLKIPVHSHSGSADQLFFNITDEMNRSSAWKAVASNTMKKSESQEKVACVRVNMEAAHNGCDKTDVETAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L1V3</accession>
    <name>A0AAR2L1V3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L1V3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041573.1">
        <property type="protein sequence ID" value="ENSPNAP00000068366.1"/>
        <property type="gene ID" value="ENSPNAG00000035738.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156785"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.460.10:FF:000001">
        <property type="entry name" value="Inositol polyphosphate 1-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.460.10">
        <property type="entry name" value="Inositol Polyphosphate 1-phosphatase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050725">
        <property type="entry name" value="CysQ/Inositol_MonoPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044897">
        <property type="entry name" value="INPP1_dom_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43028:SF3">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 1-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="372" mass="40693" checksum="BEE425932451FFE6" modified="2024-11-27" version="1">MAELLRLLLSVSEKAANVARVCRQEAALFELLVQEKTGVDKNKKFVQDFKTLADVVIQEMIRHDVCTQFPELTGFIHGEESNKFENGLGESVTITVCAKEEDTCDLLAKVLDGDQTAAVLLTKAIHQNLQIRDEAAESLQLALSPADVGIWIDPIDATSQYIEGKEQEVSDGRFCPSGLPCALVLIGVYLRDTGEPVMGVINQPFSHKDSTGKGWKGQHFWGVSYGEVNRCSFTYPKQSLKKKNDSLSVVLSSSERREVKDALAPLCNGCLKYASGAGYKILCVIQGLVDVYVLSEGSTFKWDSCAPHALLRALGGGVSDLGECLSAVSNGNKGFKAELTYHQPQAGCQGVDSRLQLHLLLRTPRLTKYYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4R9</accession>
    <name>A0AAR2L4R9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribonucleoside-diphosphate reductase subunit M2</fullName>
            <ecNumber evidence="4">1.17.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Ribonucleotide reductase small chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ribonucleotide reductase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RRM2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L4R9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051600.1">
        <property type="protein sequence ID" value="ENSPNAP00000069647.1"/>
        <property type="gene ID" value="ENSPNAG00000023397.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013305"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.620.20:FF:000004">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase subunit M2 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030475">
        <property type="entry name" value="RNR_small_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF20">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SUBUNIT M2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00368">
        <property type="entry name" value="RIBORED_SMALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="402" mass="46359" checksum="CA9EF2BE29969D27" modified="2024-11-27" version="1">MQFSGWLVSLKTASDTMLSTRSPLKAKNENGVSTKMDSLSLVDKENTPPSMSTSRILASKTARKIFADSEEQSKPKNESVGEEPLLKENPRRFVIFPIQYHDIWQMYKKAEASFWTAEEVDLSKDLQHWESLKDEERYFISHVLAFFAASDGIVNENLVERFMQEVQVTEARCFYGFQIAMENIHSEMYSLLIDTYIKDPKEREYLFNAIETLPCVKKKADWALNWIGNKDANYGERVVAFAAVEGIFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFKHLVNKPSERTVKKIIMNAVQIEQEFLTQALPVKLIGMNCDLMKQYIEFVADRLMLELGFNKIYKVENPFDFMENISLEGKTNFFEKRVGEYQRMGVMSGPTDNTFRLDADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4Z8</accession>
    <name>A0AAR2L4Z8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CTCK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sclerostin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L4Z8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050375.1">
        <property type="protein sequence ID" value="ENSPNAP00000069441.1"/>
        <property type="gene ID" value="ENSPNAG00000032700.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014900"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008835">
        <property type="entry name" value="Sclerostin/SOSTDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14903:SF7">
        <property type="entry name" value="NOVEL PROTEIN SIMILAR TO VERTEBRATE SCLEROSTIN DOMAIN CONTAINING 1 (SOSTDC1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14903">
        <property type="entry name" value="SCLEROSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05463">
        <property type="entry name" value="Sclerostin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043759076" description="CTCK domain-containing protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="10">
        <location>
            <begin position="69"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="180" mass="19662" checksum="AACC5C3C656029B4" modified="2024-11-27" version="1">MAVDTGGCQLLLFLCVVLKTCGSLHNDATELLSAHVASTLQETPSNMSLNGARDGGRSTDDQVEIGLGCRELRSTKYISDGQCTSVQAVKELVCAGECLPAHLLPNWIGGSYWARRDARSWRCVNDATRMKRVKLRCRDGSTRSYRIAAVTSCTCKRLARRHNESGPLQDSKGEKNEGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBR0</accession>
    <name>A0AAR2LBR0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBR0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065360.1">
        <property type="protein sequence ID" value="ENSPNAP00000073965.1"/>
        <property type="gene ID" value="ENSPNAG00000031664.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183016"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="11">
        <location>
            <begin position="10"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="192" mass="22123" checksum="5B44A39469103C10" modified="2024-11-27" version="1">MVMHRHLNIISLNVNGLNSPIKRAKILSKMRKEKIDVVMLQETHLGNDEHEKLKKRGVAILFSNSVYFECTSEIKDKEGRYIFVKGKIDNNEVTFCNVYAPPGERWTLFDKIFNLVATETSGILVCAGDFNLILNLDSTNQQRRSPFNMKIRKVMQELGLIDTLRNFNPSQRDYTFYSTGTPQNGIKYLKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LCP0</accession>
    <name>A0AAR2LCP0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LCP0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061908.1">
        <property type="protein sequence ID" value="ENSPNAP00000074300.1"/>
        <property type="gene ID" value="ENSPNAG00000037698.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156213"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12594">
        <property type="entry name" value="RRM1_SRSF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000690">
        <property type="entry name" value="Serine/arginine-rich splicing factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038:SF3">
        <property type="entry name" value="SPLICING FACTOR, ARGININE_SERINE-RICH 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="104" mass="11759" checksum="3CF13C4027F8EB1A" modified="2024-11-27" version="1">MSRVYIGKLSYRAREKDVEKFFKGYGKILEVDLKNGYGFVEFDDPRDADDAVYDLNGKDLCGERVIVEHTKGPRRDGGYSSGRSKLLFTLNCELKVLLNGVCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LD16</accession>
    <name>A0AAR2LD16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LD16"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078211.1">
        <property type="protein sequence ID" value="ENSPNAP00000074473.1"/>
        <property type="gene ID" value="ENSPNAG00000031342.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230338"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="182"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="306" mass="34412" checksum="7B1061D8B7A17B7C" modified="2024-11-27" version="1">MEVIGTPEFNDLDGRVNSFGNIVCIAALCSSTVTDCSPSVSLHTLLAPFYPVLQGSGPPCTKHVLFKWWIILRTAITLMWWCCVSVCCAGLSGSDTAVVLEINTTMRRYYRLTAVCLGLLCVILLAAITVLWIKFNNLTTEKDQIQTSYTNLTIERDQLQTSYTSLTIERDHCLNTCIIFCFRDSFYYISTEKKSWTESRKDCKERGRGADLVIINSRKEQEFISEAFGSSEAWIGLTDVDTEGVWKWVDKSTLTAKFWWTGEPNDYGGNEDCAVTGYEGAGSERVSTWADYPCERPAVGICEKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LHY6</accession>
    <name>A0AAR2LHY6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-J</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CCNJL</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554018.1">
        <property type="nucleotide sequence ID" value="XM_017698529.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LHY6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075757.1">
        <property type="protein sequence ID" value="ENSPNAP00000074272.1"/>
        <property type="gene ID" value="ENSPNAG00000037273.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108427939"/>
    <dbReference type="CTD" id="79616"/>
    <dbReference type="GeneTree" id="ENSGT00940000159349"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="CDD" id="cd20528">
        <property type="entry name" value="CYCLIN_CCNJ-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20529">
        <property type="entry name" value="CYCLIN_CCNJ-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000022">
        <property type="entry name" value="cyclin-J isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000036">
        <property type="entry name" value="cyclin-J isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="48"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="6">
        <location>
            <begin position="148"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="156"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="406" mass="44974" checksum="31A160699996DEBE" modified="2024-11-27" version="1">MGKMEPEGQWWKSQLAADIHQALRIKELKLPTYHAHSPQIGMRRYFADLLAVLSNRYQLCPTARHLAVYLLDLFMDHYDVAVRQLYVIALSCLLLASKFEEKEDRVPKLEQLNTLGFMCSLNLALNKRDLIKMELLLLETFGWNLCMPTPAHFIDYYLHAAVQEGDLHNGWPLTSLSKTKAFMDKYTHYFLEVSLQDHAFLSFRPSQVAAACIAASRICLQISPSWTTVLHLLTGYSWDHLTQCIQLMLLAHDNDVKEANKSKSSPSHGQVSLQAQAHASPSPAASSLQRQPASSSQQLLLQAGAFSQHSPALSQLHMLAECQALGPVGSRDYLQPHHSSLLSASLPASSFPSFPSLASGLCGLPLQGPLSMQVGVGAEPHHCVGLAYSNSYLGSHHTFTASCFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LQL4</accession>
    <name>A0AAR2LQL4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LQL4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044960.1">
        <property type="protein sequence ID" value="ENSPNAP00000077019.1"/>
        <property type="gene ID" value="ENSPNAG00000035379.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00830000128446"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="12"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="113" mass="12816" checksum="E4239EEE33A1D785" modified="2024-11-27" version="1">AGTRLACVFGADQIELSKDQKTDVFKKEGESVTLKCSYDSSSEVVFLYWYRQYPNRALQYLLYRGARSRSGNSNTADSRFESTATRISTELRTTELRLSDTALYYCALRVGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LYF5</accession>
    <name>A0AAR2LYF5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LYF5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086658.1">
        <property type="protein sequence ID" value="ENSPNAP00000079697.1"/>
        <property type="gene ID" value="ENSPNAG00000031802.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154356"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF23">
        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="2">
        <location>
            <begin position="79"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="5"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="712" mass="80474" checksum="FB4EA10A5DEA8EF1" modified="2024-11-27" version="1">MYLCRPSQVQSRNASPGRLPQTSSRKTMKGKLQVTITDQAKSILVSRGETAFRNRSAKYPASVRDRGIGGTSRSFTNALLSCSLPNGQTVTRQWLLYSPSTGCVYCFACKLFSSKHNTFVHGFSDWKHSERTGEHEGSVEHRACMLALYNRSRGTATIDSDIVKHIDAERQYWREVLKRVVAVIQFLGERGLAFRGDDELLGSAHNGNFLAPPKVSYLSSTVCEEFIHLMGQRTKQAIVNEIKESKYFSVIVDSTPDLAHVDQLTFIFRFVNNDGHVVEGFLAFEPIENHSGDSLAECVVAMVENLGLDLSNCRGQSYDNASNMSGKYNGVQAHLKQRNPLICYVPCAAHSLNLVGVNAVDSSPEAGRFFDFVQAMYTFCASSTHRWGKSTKALWKYYAQLREALNDMSKDMEEKCVTRSEASALCDKLDTLEMAFMAYFWDTILQRFQATSLQLQKHDIDICTATQLLLSLRDFVAAQRDNFEVFEGAALNVTTAVSQEYRHDLQRTKKRKIMSDDSAEPGVAFNGKDKFRIETFNVVIDQLVSCLNHRLNAYTHLTELFDVLFMPDNMSNSKLTLKANSVAAAYPSDLNMSLADELIQYKSFITEKEKHPSKMLKTMINLNLQSTFPNVYIALRLFLTVPITNCEGERSFSKLARIKNELRIRMRGFAVQLFAQGPRLSLIRPWFYVTFSVLFSKRCIQHINLKNHFSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQ41</accession>
    <name>A0A3B4BQ41_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Prominin 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PROM2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578561.2">
        <property type="nucleotide sequence ID" value="XM_017723072.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQ41"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011485.2">
        <property type="protein sequence ID" value="ENSPNAP00000001827.2"/>
        <property type="gene ID" value="ENSPNAG00000000955.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442832"/>
    <dbReference type="GeneTree" id="ENSGT00530000063586"/>
    <dbReference type="OMA" id="SLWNTLH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071914">
        <property type="term" value="C:prominosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF4">
        <property type="entry name" value="PROMININ-1-A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043354997" description="Prominin 2" evidence="8">
        <location>
            <begin position="25"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="768"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001827.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="820" mass="90910" checksum="50B10221A5261ABF" modified="2024-11-27" version="2">MGAGVRRHTLGILSLLSLWPCMTTQPSCPAGATPPELEMPGKWSVQLDSVDGGFMSLIVHSFLHSVQSNPFPKDLIIKINSGTATTETIKEVLRYQTGFLVCVAIGILYIVLMPLIGFCFACCRCCGNCGGRMYQKQTKSINCKRRVFYWATLLITLCILAGNICMFLSNTYTGDGVSTGVGEVNNTLKNLQSYIDTVPKQIDQVLNESSVTVEEVTCHINESGHFLGKQIQNGLEGPLKPALDSVRLMEQVVNRTSLLLLELNRTQLQLQTELGVLQNNLTSVRDRINDTLHKTNCLGCDLFQSNLDRLSLDTNLNTTNLTELQSAVDQAVNADLNAQINKGEELLDSIPQRVTNETRGSVQGIQQQLQNLKRQVSQVATDLPHETLNDISSQLSNVQKSFSNNVPQVKSAEQIRWIVALILCCLILLVVVCNLLGLLLGPAGLKPNDDPTDRSSTANCGGLFLMAGVGFSFLFSWIFMIVVLILFFVGGNIYTLVCVPWRSGELFQFLDTPGVIPGFQLSQNLGLKTNLTITGVYNDCHKDESLWNTLHLAEVINLNDFLNVSKYTAQVQQDFEKSQISLPNITLLSSGIQSQLRNFSTTANSVNFSSIIQKINTFSEADLKSTADELDRLTNNQANTTIKTELQNEANDLRFIQTQINITINPLVGQLKSTVERLSVLSSQVKGTVENVLQKVSYAQDVLNNNKTQIVKSKTREFIDSQMGILQKFAEWANDTITERVGRCGSVAAAVDTAENLVCTKLVDSVNAFWFSLGWCMVFLIPSIIFSVKLAKFYRQMKHKDVYENQIMMHPIPRANMKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRD9</accession>
    <name>A0A3B4BRD9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Peroxin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PEX1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550283.1">
        <property type="nucleotide sequence ID" value="XM_017694794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRD9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001004"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012902.2">
        <property type="protein sequence ID" value="ENSPNAP00000001004.1"/>
        <property type="gene ID" value="ENSPNAG00000000530.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425814"/>
    <dbReference type="CTD" id="5189"/>
    <dbReference type="GeneTree" id="ENSGT00550000075032"/>
    <dbReference type="OrthoDB" id="8173462at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19526">
        <property type="entry name" value="RecA-like_PEX1_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000966">
        <property type="entry name" value="Peroxisomal biogenesis factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001852">
        <property type="entry name" value="Peroxisomal biogenesis factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000060">
        <property type="entry name" value="peroxisome biogenesis factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000004">
        <property type="entry name" value="Peroxisome biogenesis factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015343">
        <property type="entry name" value="PEX1-N-lobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015342">
        <property type="entry name" value="PEX1-N_C-lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF12">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09262">
        <property type="entry name" value="PEX-1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09263">
        <property type="entry name" value="PEX-2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="570"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="853"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1186"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1242"/>
            <end position="1263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1263" mass="138772" checksum="659AE6224AFBBA1C" modified="2018-12-05" version="1">MLGSCGIQPVTLVFNNSKNCFLHLPSKLVTQLSLQESQALELSWGCAAPVFLSWIRSHTSLQENRVELSRQLGEKLGLQEGEQGFLRPCGQVQSVQQVFVEPLSSDDWEILELHSTALEQQLLDQIRVVFSSGVFPVWVDHHTIIYIKIVSISPSVSYGRLEQFTELVVSPKLRCGERQLPPVAPDPKSQVLQRQQNFDLTSSSTTSSLGTDSVHDPFIDQLEFSQSHYGGHWGGIADLRSLVRYVFTGKFEPAKEIPEVPTIPVIFPDCVLRVSRSPPSAIRHLDSSHRDVHLLPWDYLQYEYRSSQQSVLTYARLSKVHSPKEAREQVKLTMEKKKSGNAPQTENDVQEHMKESVVVRMLCHNVEKLQADQKSSRMGEIYCGRVWIPNILRSRLNIDPHSAVKVCPIKCIPKVALAVSLQPLQPLAESEREEDVQSAFLGWLHALSHQPLACLTGRTNIILLRCTEGNYEFVLTVLKPEPETPKQVDEIFFLSTSVLCKPDIQVVKEHHTNHTSSVQTADESPCVGFPSLSSLGGVDDISASAFEHIFHSLMGGPLSWELVSSGCGLRSGALLITGAKGSGKSALSRALCRKAAEELDAHVEVVDCIKLKGKRADTVRQRLEEVFEQAVWRQPSVVLLDDLDHVVGAATSPEQEQGTEAVLRQHIALSLMDLVDEVVVRSSLVALLVTAHSEHSLHPMLTQVQGTHFFQSFCKIQTPDQIKRVDILRSLMDSKNVISENSVMNLDLDTIAMKTEGYLPRDLNLLLDRAIHASIAHNRGSDTVQGLCLSSKDFQQALQGFTPPSLWGVQLQTPSGMGMEWVGGLHQARQLLIDTILLPAKYPVLFSSLPIRQRSGVLLYGAPGTGKTLLAGAVAKDSGMNFISIKGPELLSKYIGASEQAVRSVFQRAQAAKPCILFFDEFDSLAPRRGHDSTGVTDRVVNQLLTQLDGVEGLQGVYVLAATSRPDLIDPALLRPGRLDKSVYCPPPDRESRVEILKALTHSVPLATDVDLEQIAVATELFTGADLKALLYNAQLEAIHSSLGPSLLPDLGSGSDSDVSLSSLIFLNHSSGSDDSAGEGEAGLESSAAVLLEHSELAADDPRHNIWRLYFGSSFELELGNHSPSELNSQCLSVQNSTTHDLTGASVRDTGCLYGPVIMTSLQEGFQEQSHEQAERLRADLNTIKSSYRRSPEDSSSVSTGPSKPGLLICQTHMTSALASTRASISRQDWRRYSELYESFGASREGKPQNTGLFTPGQRVTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BU40</accession>
    <name>A0A3B4BU40_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017568539.1">
        <property type="nucleotide sequence ID" value="XM_017713050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BU40"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002010"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011177.2">
        <property type="protein sequence ID" value="ENSPNAP00000002010.1"/>
        <property type="gene ID" value="ENSPNAG00000001060.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436519"/>
    <dbReference type="CTD" id="567919"/>
    <dbReference type="GeneTree" id="ENSGT00940000156445"/>
    <dbReference type="OMA" id="YLGHYPH"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14071">
        <property type="entry name" value="STKc_SIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14409">
        <property type="entry name" value="UBA_SIK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000154">
        <property type="entry name" value="Serine/threonine-protein kinase SIK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034672">
        <property type="entry name" value="SIK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057380">
        <property type="entry name" value="UBA_SIK1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF38">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23312">
        <property type="entry name" value="UBA_SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="21"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="18">
        <location>
            <begin position="298"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="551"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="742"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="839"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="890" mass="98999" checksum="9CB2A6F648588A4E" modified="2018-12-05" version="1">MVMADCHQKAALRAPVRVGFYDIERTLGKGNFAVVKLARHRITKTEVAIKIIDKTQLDSVNLEKIYREVKIMKMLDHPHIIKLYQVMETKNMLYLVTEYAKNGEIFDYLAKHGRLSEPEARRKFWQILSAVEYCHNCSVVHRDLKAENLLLDGHMNIKIADFGFANFFQTGEPLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPSLPVLRQRVLEGRFRIPYFMTEDCEHLIRRMLVLDPSKRLSVAQIKEHKWMVLEVPVQRPLLYQQTGQAEGEPGLGEYSEQVLRLMHSLGIDQHKTVESLQNKSYNHFAAIYYLLVERLKAHRCSFPVEPRLDARQRRPSTIAEQTVVKSSSGAPQVSLLTQNVRLLRSPAVAQASADSYSFPQSPCAPEHSLMEEEVGTPKVDGCMLDPLPPLTVRKSSTSSPSNMMETSIDEGIETEDPDADEDPAHIFSAYQTARFGQRRHTLSEVTNQPGVLTSAGKMFSLGHNPSLGSVDSEYDMGSMHSDLSLLEDTSSLNEVVLATTPISRVTPPFARPPNPAMQALSSQRREAHNRSPVSFREGRRASDTSLTQGLVAFRQHLQNLARTKGILELNKVYEQMGSGENPSLGPLSPQTHPHDLLDPLIQGEGGHQHSMALFSGGPQPLLSRRQSLEPQYPSHRIPKPSLMASPNSCQMFCKETPRSLEQQLAEHRLQQKRLYLQKQSQMQAYFNQMHIVEGPYAPCTPMSSQDSAAPPQVQGSIAPLHQQQSPQASPPYTPHPQTFSPLMEPYLGHYPHLQPSALPPGLQGQQPEALSYGYPVHEPGAQCSAGDVLYQEQYSHPLDPGQPPPPGAAAGAPASGYEGLAIADPFLDSEMMETVDSQTGFVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BV40</accession>
    <name>A0A3B4BV40_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Exosome RNA helicase MTR4</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Superkiller viralicidic activity 2-like 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">TRAMP-like complex helicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">MTREX</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BV40"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002369"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010557.2">
        <property type="protein sequence ID" value="ENSPNAP00000002369.2"/>
        <property type="gene ID" value="ENSPNAG00000008794.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156183"/>
    <dbReference type="OMA" id="IMLKNYN"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18024">
        <property type="entry name" value="DEXHc_Mtr4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13154">
        <property type="entry name" value="KOW_Mtr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000141">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.300:FF:000001">
        <property type="entry name" value="Mtr4 exosome RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000002">
        <property type="entry name" value="superkiller viralicidic activity 2-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000004">
        <property type="entry name" value="Superkiller viralicidic activity 2-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0347">Helicase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="18" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="10" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="25">
        <location>
            <begin position="138"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="26">
        <location>
            <begin position="400"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00069430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00077324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002369.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1041" mass="117559" checksum="A7F2BF4DA16694DE" modified="2024-11-27" version="2">MADAFGADLFSVFEEEQSSSSKKTSTKTAAQPGKTASTHGKEKTESPSLSRAKREADNDGTEEVVFGKKAKLETVSSEEINLADLMPKVKVEQVETVEGCTHEVALPADEEYKQLKPRVGKAAKEYPFILDPFQREAILCIDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVGLMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYVFLSATIPNARQFAEWICHLHKQPCHVVYTDYRPTPLQHYIFPAGGDGLHLVVDENGEFREDNFNTAMQVLRDAGDQGGSSGGKWDPKGRRGGTKGPSNVFKIVKMIMERNFQPVIIFSFSKKECEAYALQVSKLDFNTDEEKKLVEEVFSNAIDCLSDEDKKLPQVEHVLPLLKRGIGIHHGGLLPILKETIEILFSEGLLKALFATETFAMGINMPARTVLFTSARKFDGKDFRWISSGEYIQMSGRAGRRGMDERGIVIFMVDEKMSPAVGKQLLKGSADPLNSAFHLTYNMVLNLLRVEEINPEYMLEKSFYQFQHYRAVPGVLDKMKNLEEMYNAIEIPNEDSVVTYYKIRQQLAKLGKEIEEYIRKPKYCLPFLQPGRLVKVKKEELDFGWGVVVNFSKKSNVKASTGDLDPLYVVEVLLHCSKDSVKNAATEAAKPTAAGEKGEMQVVPVLLNLLTSFSSVRLYIPKDLRPYDNRQSMLKSIQEVQKRFPDGLPLLDPIDDMGIKDPGLKKVIQKVEAFEHRMYTHPLHSDPNLEAVYTLCEKKALVAGDIKSAKRELKKARTVLQMDELKCRKRVLRRLGFATSSDVIEMKGRVACEISSADELLLTEMIFNGLFNDLTAEQATALLSCFVFQENANEMPKLTEQLAGPLRQMQECAKRIAKVSAEAKLEVDEETYMNQFRPHLMDVVYTWANGASFSQICKMTDVFEGSIIRCMRRLEELLRQMCQAAKAIGNTELENKFALGMFHRQNLDADCILAVRLHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWH0</accession>
    <name>A0A3B4BWH0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleosome assembly protein 1-like 4a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573021.1">
        <property type="nucleotide sequence ID" value="XM_017717532.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWH0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003958"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007834.2">
        <property type="protein sequence ID" value="ENSPNAP00000003958.1"/>
        <property type="gene ID" value="ENSPNAG00000010389.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439256"/>
    <dbReference type="CTD" id="337155"/>
    <dbReference type="GeneTree" id="ENSGT00940000169159"/>
    <dbReference type="OMA" id="HELEWSY"/>
    <dbReference type="OrthoDB" id="27325at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="317"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="317"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="41747" checksum="AE9C17A0DD9C2D9E" modified="2018-12-05" version="1">MEARKNEKNEARGKGGNKASQGDRTEIISEAERIPNVSALPKAVKRRVYALKRLQVQSAHIEAKFYEEVHELERKYAGLYQPIFDKRREVVSGAVEPTDEECEWHSEGEEEELAEDLKKKAALEEKKEESAPADDPKGIPEFWLTIFKHVDMLGEMLQEHDEPILKHLQDIKVKFSEPGQPMSFTLEFHFEPNSYFSNAVLTKVYKMKSEPDPVDPFSFEGPEIVDCEGCKIDWHKGKDVTVKTIKKKQKHKGRGTVRTITKEVPQDSFFNFFNPVKASPDGMDEDLDFTLATDFEIGHFFRERIIPRAVLYFTGEALEDDESFEEEDLEEGEEEDLDEEGEEEDEGDLDPTKEPQPAECKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BY91</accession>
    <name>A0A3B4BY91_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">TGF-beta activated kinase 1 (MAP3K7) binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TAB3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017580940.1">
        <property type="nucleotide sequence ID" value="XM_017725451.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017580941.1">
        <property type="nucleotide sequence ID" value="XM_017725452.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017580942.1">
        <property type="nucleotide sequence ID" value="XM_017725453.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017580943.1">
        <property type="nucleotide sequence ID" value="XM_017725454.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BY91"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004548"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006818.2">
        <property type="protein sequence ID" value="ENSPNAP00000004548.1"/>
        <property type="gene ID" value="ENSPNAG00000001480.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444322"/>
    <dbReference type="CTD" id="257397"/>
    <dbReference type="GeneTree" id="ENSGT00940000159499"/>
    <dbReference type="OMA" id="WACNLCT"/>
    <dbReference type="OrthoDB" id="6288762at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000006">
        <property type="entry name" value="TGF-beta activated kinase 1 (MAP3K7) binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46253:SF3">
        <property type="entry name" value="TGF-BETA-ACTIVATED KINASE 1 AND MAP3K7-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46253">
        <property type="entry name" value="TGF-BETA-ACTIVATED KINASE 1 AND MAP3K7-BINDING PROTEIN TAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CUE" evidence="9">
        <location>
            <begin position="8"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="8">
        <location>
            <begin position="554"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="83"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="520"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="429"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="64493" checksum="6A2C0389E6A43405" modified="2018-12-05" version="1">MAQAGPQLDYHILQDLKQRFPEIPENVVSQCLLQNNNNLDLCCHLLAQESNRYLYEEYHSPEEVHLSRNHMLHISVGYPPPEGAKASTAGGRQLVHSSSDGHIEPQRGAFPEPHSAPAAMAPSPGYNPFFMGDQGRSASTPTPPPTMQGMSPTYTPVPQRYMTPITVTLSQNIPNVPQALQIPPGAYGNSGNALYMRPSPSQSPQPTPWATSAAPVYQPSPYTTPTYQSPYSSPQHQVQQPPHVFLPISPPTLSNMPYQQAPPVSYRPYMTSKGSMKNQIEITLEGRPRSNSPVHAPQGALYMATSTSPSSPSRAISMTGPPAFHPGVYLHQGAARPRPASSPQAASSAYIKIKVSPGQAQRSLESPPVETESLLNIVDQGENHGTPAPILPISALPGNVASQISHMPRRSSSGSDDYAYTQALLLHQRARMERLMKELLVEKQKLELLKAEVNDMEYDALQRRFRRVNSTSFIPRPEEMTRMRSQNRQLQIDIDCTLKETDLLQSRGKFDPKAMNNFYDNIQPGPVVPPKPGKRDGGGRSVKPSVEAQRDEDFEGAQWNCDSCTFLNHPALNRCEQCEMPRYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYL0</accession>
    <name>A0A3B4BYL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539278.1">
        <property type="nucleotide sequence ID" value="XM_017683789.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYL0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004673"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006610.2">
        <property type="protein sequence ID" value="ENSPNAP00000004673.2"/>
        <property type="gene ID" value="ENSPNAG00000001524.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411966"/>
    <dbReference type="CTD" id="337243"/>
    <dbReference type="GeneTree" id="ENSGT00940000157195"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000143">
        <property type="entry name" value="septin-9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="10">
        <location>
            <begin position="318"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="245"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004673.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="608" mass="68248" checksum="7A21C5F86236D29A" modified="2024-11-27" version="2">MRKSSTSGIARSTNSRVRRLERADSAGAAGAALKRSFEVEEVDSPTNPSPLSRRTANPLRSSAGSASSRFHDLASRNSDYASRHGSETLSSGQRSPKSSLRRAELTGARLPEAASRRTEISIDISSKQVDSSPSPGIARFGLKRPEVSLQSRTPELAAHRRTEITLGRSQDAPASLRRADGPAPTSRIPEPAQRKTEPPSPVDTSPMTRRPENLAVRQTETPFPARESENSIISAASVTPSFTEYKAPLPTHENTSRAPERSEFISSYSSNSSSSSSMAEPVISEMPPTACPEKPNVDFSYVGIDAILEQMRRKAMKQGFELNIMVVGQSGLGKSTLMNTLFKSKVSRKSVMGTAEERIPKTIEIKSISHDIEEKGVRMKLTVIDTPGFGDQINNENCWQPIMKFINDQYEQYLQEEINIERKKRIPDSRVHCCIYFIPPTGHCLRPLDVEFMRRLSKVVNIVPVIAKADTLTLEERDFFKKKIREELRANSIDVYPQKEFDEDAEDRLINEKIREMIPFAVVGSDQEYQVNGRRLLGRKTKWGTIEVENIAHCEFAYLRDLLIRTHMQNIKDITSSIHYEMYRVRRLNENNLQANGLAEHHPASHEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZ15</accession>
    <name>A0A3B4BZ15_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sodium-dependent phosphate transport protein 2A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Na(+)-dependent phosphate cotransporter 2A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sodium/phosphate cotransporter 2A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Solute carrier family 34 member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Involved in actively transporting phosphate into cells via Na(+) cotransport in the renal brush border membrane. The cotransport has a Na(+):Pi stoichiometry of 3:1 and is electrogenic.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3 Na(+)(out) + phosphate(out) = 3 Na(+)(in) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71255"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:71256"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Interacts via its C-terminal region with NHERF4. Interacts with NHERF1. Interacts with TMEM174; regulates SLC34A1 internalization by PTH and FGF23.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC34A transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559326.2">
        <property type="nucleotide sequence ID" value="XM_017703837.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559327.2">
        <property type="nucleotide sequence ID" value="XM_017703838.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZ15"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003724"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008237.2">
        <property type="protein sequence ID" value="ENSPNAP00000003724.2"/>
        <property type="gene ID" value="ENSPNAG00000010150.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430989"/>
    <dbReference type="CTD" id="100329340"/>
    <dbReference type="GeneTree" id="ENSGT00950000183177"/>
    <dbReference type="OrthoDB" id="76259at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005903">
        <property type="term" value="C:brush border"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005436">
        <property type="term" value="F:sodium:phosphate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044341">
        <property type="term" value="P:sodium-dependent phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003841">
        <property type="entry name" value="Na/Pi_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01013">
        <property type="entry name" value="2a58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037997">
        <property type="entry name" value="Na_Pi_symport"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010:SF21">
        <property type="entry name" value="SODIUM-DEPENDENT PHOSPHATE TRANSPORT PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 34 SODIUM PHOSPHATE , MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02690">
        <property type="entry name" value="Na_Pi_cotrans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="462"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003724.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="666" mass="71073" checksum="555CB7F10FB70B8A" modified="2024-11-27" version="2">MSSRPIRIISTGTGKMSSFNGMNLHSSRASECAECDMGSTVDLISSCSHLHSSGPGSPSHETWDGGRLPKIQVLPEAPHGEKTKMKSLFISVMKIPLLFFLLYCFVCSLDTLSSAFQLAGGKVAGNIFQDNAILSNPVAGLVVGILVTVLVQSSSTSTSIIVSLVSSGLLEVKSAVPIIMGSNIGTSVTNTIVALMQAGERSEFKRAFAGATIHDCFNWLSVLVLLPLEVATGLLSFLAQAVVSSFQLQSGEEAPELLKVITDPVTKLIIQLDKSVITGIALGAEHMRNRSLVKVWCGSSIYTPSANDSLNCSSVFDCKTALTSQGTAERCHHLFANTNLSDLSVGLILLAVSLLVLCSCLILIVKVLNSVLKGQVAKIIQVIINTDFPYPFSWLAGYVAMLVGAGMTFLVQSSSVFTSAMTPLIGLGVITLERAYPLTLGSNIGTTTTAILAALASPGDKLPAACQIALCHFFFNAFGILLWYPLPFSRLPIRMAAALGERTAKYRWFAVLYLLLCFLLLPSLVFGLSLAGWQAMVGVGAPFAGVATFIVLVNLLQSHSPAHLPKKLRSWDFLPHWMHSLKPLDKLITNATAMCCSPARSTTAATVDPEAPGSPDTTNVKSASVMFQGKVQLAFDNPALSYKDESPPQPRVFKLKGLERCNSTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0Z9</accession>
    <name>A0A3B4C0Z9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphatidylcholine-sterol acyltransferase</fullName>
            <ecNumber evidence="13">2.3.1.43</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Lecithin-cholesterol acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Phospholipid-cholesterol acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LCAT</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a sterol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a sterol ester + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:21204"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:35915"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="2.3.1.43"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.43" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017554326.2">
        <property type="nucleotide sequence ID" value="XM_017698837.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0Z9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005607"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005024.2">
        <property type="protein sequence ID" value="ENSPNAP00000005607.2"/>
        <property type="gene ID" value="ENSPNAG00000001919.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428118"/>
    <dbReference type="GeneTree" id="ENSGT00940000160052"/>
    <dbReference type="OMA" id="VVNWLCY"/>
    <dbReference type="OrthoDB" id="190846at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004607">
        <property type="term" value="F:phosphatidylcholine-sterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000090">
        <property type="entry name" value="Phosphatidylcholine-sterol acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000183">
        <property type="entry name" value="Phosphatidylcholine-sterol acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="9" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043377483" description="Phosphatidylcholine-sterol acyltransferase" evidence="18">
        <location>
            <begin position="23"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005607.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="445" mass="51054" checksum="353C59D5FCDEAE4D" modified="2024-11-27" version="2">MQACRALSSVIVVLVALHECGAFWLLNVIFPPPAKPRVPSNSTPPVIIVPGKLGNRLEAKIDKPTLVHWLCYKKTEDWFPLWIDLNMFMPIGIDCWIDNIRIVYNRTTHKTSNAPGVEVRAPGFGQTYTVEFLDSNKLAGYFYTMVQHLVNIGYVRNETVRAAPYDWRIAPNEQEEYFSRLKGLIEEMHDEYKQPVHLLGHSMGSNYILYFLNQQSQAWKDRYIKSFISLGAPWGGAVKPLRVLASGDNDGIPLVSNIKIREEQRMTTTNPWMIPSDDAWPKDHVFISTPFFNYTNQDYQRFFTDINFEDGWYMWEDTRNLTAGLPTPGVEVYCFYGVGLPTPVTYIYDEEFPNSNPIDFLYDDGDDTVDSRSMGMCKRWIGQQEQPVYVTEFKGMPHLDMVFNHNVLTAIQEILEGTFHQEESVHTVTVKASTNLPQEPLTPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C750</accession>
    <name>A0A3B4C750_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Synaptotagmin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="13">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Chromaffin granule membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Cytoplasmic vesicle</location>
            <location evidence="1 13">Secretory vesicle</location>
            <location evidence="1 13">Synaptic vesicle membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C750"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006721"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003071.2">
        <property type="protein sequence ID" value="ENSPNAP00000006721.2"/>
        <property type="gene ID" value="ENSPNAG00000012718.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155394"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042584">
        <property type="term" value="C:chromaffin granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08402">
        <property type="entry name" value="C2B_Synaptotagmin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21963">
        <property type="entry name" value="Syt1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000007">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000016">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF239">
        <property type="entry name" value="SYNAPTOTAGMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="154"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="285"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006721.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="434" mass="48892" checksum="AEECD18B094855DE" modified="2024-11-27" version="1">AIMLTNWHSVAVMLAFYSSKTRREALVGNPAPSAGPETLSNSTEPAAPGGGGPKDEAFSKLKDKFMNELNKIPLPSWAIVAIAFVAVVLVVSCCFCICKKWIFKKKNKKKGKDKGKNAINMKDVIDGTKTEDEDEVETGLTENEKEAEPKEQEKLGKLQYSLDYNFTENTLIVGIIQAAELPAMDMGGTSDPYVKVYLLPDKKKKFETKVHRKTLNPTFNEQFTFKVPYTELGGKTLVMTVYDFDRFSKHDAIGDVKVPMNKVDFSHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQIVITVLDYDKIGKNDAIGKVFVGLNSTGTELRHWSDMLANPRRPIAQWHVLKPEEEVDAQLAVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CB58</accession>
    <name>A0A3B4CB58_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Insulin-like growth factor 2 mRNA binding protein 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CB58"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000707.2">
        <property type="protein sequence ID" value="ENSPNAP00000008116.2"/>
        <property type="gene ID" value="ENSPNAG00000013765.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154913"/>
    <dbReference type="OMA" id="TSGPHRT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22497">
        <property type="entry name" value="KH-I_IGF2BP2_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22500">
        <property type="entry name" value="KH-I_IGF2BP2_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.210:FF:000001">
        <property type="entry name" value="insulin-like growth factor 2 mRNA-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="65"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="146"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="297"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="379"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008116.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="471" mass="51097" checksum="54D64DE70E7954EF" modified="2024-11-27" version="2">MPKLYVGILSSSVTVEDLQRLFPRKLPKAHQAAPSPPSCRQRRLGLQDPQPGPSVNPLGSRHSLPNLPLRILVPTQFIGAIIGKEGLTIKNLTRQTQSRVDIHRKENAGAAETPITIRSTPEGCSAACRMILNIMQKEAYDTKVSAEIPLKILTHNGLVGRLIGKEGRNLKRIEEESGTKITISSLQDLTLYNPDRTITVRGSVDVCCRAEVEIMKKLREAFENDASVNQSSGLIPGLSLSTQGIFSSGLSMLPSTSGPHRTLQTAYGPFTGQSACLGGLYGVSSAAPHAHLHTPPEQEVVYLFIPTQAVGALIGKKGQHIKQLTQFAGASIKIAPAESPDVTERMVIITGPPEAQFKAQGRIFGKLKEENFFSSKEEVKLETHIKVPSSAAGRVIGKGGKTVNELQNLTSAEVVVPRNQTPDENDEIFVKISGHFFASQTAQRKIREIIQQAKLHKQKHQLGASGSHQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CC50</accession>
    <name>A0A3B4CC50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="4">2.7.7.49</ecNumber>
            <ecNumber evidence="3">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="4"/>
    <dbReference type="EC" id="3.1.26.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CC50"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008456"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000112.2">
        <property type="protein sequence ID" value="ENSPNAP00000008456.2"/>
        <property type="gene ID" value="ENSPNAG00000014076.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240511"/>
    <dbReference type="OMA" id="CAANTSW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08284">
        <property type="entry name" value="RVP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="14" id="KW-0229">DNA integration</keyword>
    <keyword evidence="18" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="22">
        <location>
            <begin position="575"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="21">
        <location>
            <begin position="1280"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1327"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1329"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1343"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1393"/>
            <end position="1407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008456.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1448" mass="160543" checksum="243A683597AB5F69" modified="2024-11-27" version="2">MAIFQSVSSDAVSLPALDSVTSMDSNSQHALPMDYSNSAEHVTLRRSGSPFVTEYSAIMETADTEALLSGLTNHDQVLGQHQQTLASLNQSVGELTNRQNNQSIQLTQIFAGLQSLTTNLQSHLHDQTVGSVSVASGHFFPICKPEPYDGNPNKCSGFILQCSVYFSNSPPSTDQSKIAFIISCLTGKALDWATASWSFLQTLSYSDFLCQLRLVFDHAHEGQSCGEQLLSLQQGSSSVSEYALRFRTLAAGSGWNEPALLTVFRDGLNSRVQSELACKDDGLKLDQLISLSLMSCDRTRLTPEERQRRFLNHLCLYCGGSGHVKASCELRPPLSSASSWGQRVGFTPRANAVSVPTKGLLSRALVLDMLITYQTLSLPVPALIDSGAEGNFICADLIQRLGVPTEELRTPLRALALDGKPLGCVPITLQTLPVTLQASALHSEEISFFVLPSTDFPLVLGFPWLERHDPVISWHSRELVSWSPYCFENCLFPGHITLSSTTIESPDSLNPVVIPPEYSDFLEVFSPVKATKLPPHRPYDCAIDLVEGAVLPKSRVYPLTLEEEKAMDVYVTEALAQGYIRPSKSPVGSGFFFIKKKDGGLRPCIDYRGLNDITKKYAYPLPLIPVALEQLRGASYFTKLDLRSAYNLVRIREGDEWKTAFTTTRGHYEYLVMSYGLSNAPSVFQAFMNDVFREFLNKFVIVFIDDILIFSPDFSSHVNHVRQVLLRLQENNLYRVSFLGYVISSSGVLMSDDKVSAVTNWPKPGSVKELQRFLGFANFYRRFIRNFSSTAAPLTALLKGSPKKLSWSDSAQGAFDALKLAFSTAPILKHPDPEKPFVVEVDASNTGVGAVLSQRSGIPLKLHPVAFFSRKLSPAERNYGIGDRELLAVKLALEEWRHWLEGAAHPFTVLTDHKNLEYLKSAKRINSRQARWSLFFSRFDFSISFRPGQRNTKADALSRIFAVEEEEESSDPERILPPTVQIAVIQWEFDEQIQQVNAHRTPPEGTPPGKLFVPPQFRNRLITWAHSSLTSGHPGETRTLQLLSARYWWDSLRSDVHSFVSSCPVCAQCKIPRTLPAGKLVPLPVPERPWSHIAIDFVTDLPPSNGFTSILTVVDGFSRGVKFIPFPSLPSALQTAQALFEHVFRSSGIPAVDDWMRRSEGVWEDTHRRISDILQRYKAQADRHRGDTPVYCPGDRVWLSTRDFRFEGACRKLLPKFIGPFKILSQVNDVTYKVGLPPQYRVNNSFHVSLLKPVVSGPLAEDDPIDVPPVSERMEDSSTYSVREILDSRRRGGNLQYLIDWEGYGPEERCWVAARDVLDPGLVADYHARRPNRPAPRPRGRPRGSLSGSSQAPRGSARARARQSSSSAGPVVAPHSSSGVSVGRRRGRPRIRPAPVSSGGGSVSSDAVSLPALDSVTSMDSNSQHALPMDYSDSAEHVTLRRSGSPCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFM7</accession>
    <name>A0A3B4CFM7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">LIM homeobox transcription factor 1-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">LIM/homeobox protein LMX1A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFM7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016684.2">
        <property type="protein sequence ID" value="ENSPNAP00000010110.2"/>
        <property type="gene ID" value="ENSPNAG00000015633.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157774"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071542">
        <property type="term" value="P:dopaminergic neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09371">
        <property type="entry name" value="LIM1_Lmx1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000095">
        <property type="entry name" value="LIM homeobox transcription factor 1 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000040">
        <property type="entry name" value="LIM homeobox transcription factor 1 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000006">
        <property type="entry name" value="LIM homeobox transcription factor 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF118">
        <property type="entry name" value="LIM HOMEOBOX TRANSCRIPTION FACTOR 1-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 16 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 16 18" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 17" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 16 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 17" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043915735" description="LIM homeobox transcription factor 1-alpha" evidence="20">
        <location>
            <begin position="26"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="21">
        <location>
            <begin position="55"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="21">
        <location>
            <begin position="114"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="22">
        <location>
            <begin position="212"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="16">
        <location>
            <begin position="214"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="181"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="271"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="276"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00084001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010110.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="411" mass="45728" checksum="C87479BF0E7E1473" modified="2024-11-27" version="2">GKKALNTALLNVLCCFVLFSQRSDRGRNGMKTEENQSCLQQAPSTTPFEHVGGGEVCAGCESPIADRFLLRVNDQSWHETCVKCAVCLSTLSGTCYCRDRLLYCKHDYEKLFVRKCSACLQVIGRSELIMRVLGQVYHLGCFSCCECERRLQRGDEFVLKEGQLLCRGDYEKEREMLAAISPAPTESVKSEDEEGGNGSVGGKAGDDGKEHKRSKRPRTILTTQQRRAFKASFEVSSKPCRKVRETLAAETGLTVRVVQVWFQNQRAKMKKIARRQQQQQQQQEQEQIGGSRRGPSRGGRQSNDDSEDGSSSHGLDSMLSYSSMSRQQLLAIDPNIYGAEQFRHGLTPPQLGPEQMHPYDSETVFHDLDSDGSLSHLGDCLLATAEGGVVAGRVGNPIDRLYSMQNSYFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFP4</accession>
    <name>A0A3B4CFP4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U5 small nuclear ribonucleoprotein TSSC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TSSC4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Protein associated with the U5 snRNP, during its maturation and its post-splicing recycling and which is required for spliceosomal tri-snRNP complex assembly in the nucleus. Has a molecular sequestering activity and transiently hinders SNRNP200 binding sites for constitutive splicing factors that intervene later during the assembly of the spliceosome and splicing. Together with its molecular sequestering activity, may also function as a molecular adapter and placeholder, coordinating the assembly of the U5 snRNP and its association with the U4/U6 di-snRNP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TSSC4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559022.1">
        <property type="nucleotide sequence ID" value="XM_017703533.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559023.1">
        <property type="nucleotide sequence ID" value="XM_017703534.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559025.1">
        <property type="nucleotide sequence ID" value="XM_017703536.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559026.1">
        <property type="nucleotide sequence ID" value="XM_017703537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFP4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009691"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016124.2">
        <property type="protein sequence ID" value="ENSPNAP00000009691.1"/>
        <property type="gene ID" value="ENSPNAG00000015157.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430813"/>
    <dbReference type="CTD" id="10078"/>
    <dbReference type="GeneTree" id="ENSGT00390000011846"/>
    <dbReference type="OMA" id="RMAAMEF"/>
    <dbReference type="OrthoDB" id="1906282at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029338">
        <property type="entry name" value="TSSC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13445">
        <property type="entry name" value="TUMOR SUPPRESSING SUBTRANSFERABLE CANDIDATE 4 TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13445:SF3">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15264">
        <property type="entry name" value="TSSC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="179"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="306" mass="34031" checksum="253A861EA51BA2BD" modified="2018-12-05" version="1">MSKSKSQDDTVNSLSNRDAVDVPDDLSLSDSDPEESLETFGRKIEDLSSSSEEEESTKHGHTVPQEQPTFRLSGGSSGFTDRSRSIFDQLDSAAKLTSNQLADDNVLDGTFARPAPPSPPQSAQRKFGGTKKVSSAKRIPDYLAHPERWTCYSLEDVPETSDKQNSQVAHEFIRGLQERRRSLEATEEPFTPAFNQDHSSSSDHKIIFTKPSQASKDEGKTANKVERAKKSEVGLGHLDAGQEDEEGGKVASLHHNSKFVEEEKRKRKRAPEEEGEQGKARNVAFNSGKKVNRKNFRKVVEEEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGE4</accession>
    <name>A0A3B4CGE4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PSI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PLXDC2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037390823.1">
        <property type="nucleotide sequence ID" value="XM_037534926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGE4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011057"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017978.2">
        <property type="protein sequence ID" value="ENSPNAP00000011057.2"/>
        <property type="gene ID" value="ENSPNAG00000016682.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442949"/>
    <dbReference type="GeneTree" id="ENSGT00440000033408"/>
    <dbReference type="OMA" id="LKAQPTC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031152">
        <property type="entry name" value="PLXDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055:SF11">
        <property type="entry name" value="PLEXIN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055">
        <property type="entry name" value="TUMOR ENDOTHELIAL MARKER 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043713978" description="PSI domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="11">
        <location>
            <begin position="309"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="64"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="375"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="390"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011057.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="512" mass="57229" checksum="B9E67ABC02D4294B" modified="2024-11-27" version="2">MARGTELLLLGLAVVVGSVEVQREREHGVSYTTLQGAAQADDVTVEVLRRKRWLVSEPRPMTNNRASLESAEDLPGVLDDSEADNSSQIEEDTDHNYYTSKTYGPNDPMSKELWVNIDQMDKEKVKIHGILSNTHRQAARVNLSFDFPFYGHFLREITVATGGFIYTGDVVHRMLTATQYIAPLMANFDPSLSRNSTVIYFDNGTALVVQWDHVHLQDSYSLGSFTFQATLHNDGRIIFAYKEVPIQITKISSVNHPVKVGLSDAFVVVHRIQQIPNVRRKTIYEYHRVELLKNKITNSTAVEMMPLPTCLQFTSCSSCIAAEINFNCSWCHRLNRCSSGFDRHRQDWVDNSCPDETKDNTCDISTTQFQSSTAASVTKATTRNRAATEADSTQTTSHGPTSAPTEDDTKIALHLKDNGAAAAESTAERDTHPLHTGLILGMLLLMLVMMAVVLVTVYIYHHPSSAAGIFFIERRPSRWPAMKFRRGSGYPAYAEVEPVGQEKEGFIESEQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNX8</accession>
    <name>A0A3B4CNX8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017568037.1">
        <property type="nucleotide sequence ID" value="XM_017712548.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNX8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012244"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019484.2">
        <property type="protein sequence ID" value="ENSPNAP00000012244.1"/>
        <property type="gene ID" value="ENSPNAG00000018020.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000073197.1">
        <property type="protein sequence ID" value="ENSPNAP00000081728.1"/>
        <property type="gene ID" value="ENSPNAG00000018020.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436204"/>
    <dbReference type="CTD" id="654827"/>
    <dbReference type="GeneTree" id="ENSGT00940000157294"/>
    <dbReference type="OrthoDB" id="6066926at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904862">
        <property type="term" value="P:inhibitory synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000017">
        <property type="entry name" value="leucine-rich repeat LGI family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009039">
        <property type="entry name" value="EAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005492">
        <property type="entry name" value="EPTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051295">
        <property type="entry name" value="LGI_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24367:SF21">
        <property type="entry name" value="LEUCINE-RICH REPEAT LGI FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24367">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03736">
        <property type="entry name" value="EPTP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50912">
        <property type="entry name" value="EAR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044589703" description="LRRCT domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="8">
        <location>
            <begin position="162"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="540" mass="61518" checksum="5BDDA2122DD20473" modified="2018-12-05" version="1">MVSTVKQCAVMSAMLLWLMPSVHSGKKVFKCLSSCSCSKESIICVGSSYVPRVTPNDVSSLSIVNGTFSEVKEAMFAHMPSLQLLLLNSNALTTIRDDAFAGLPHLEYLFIENNKIETTSKYSFRGLRDLTHLSLANNNIKALPRDLFIDLDSLIELDLRGNVFECDCRAKWLMMWLKNTNATVSDVLCAGPEEMKGKRLNDMASLHNECISTDFIPLHSVTTESLSVDTFSHKNDVYVAIAAPNIESCMVLQWDHIEMNFRTYDNITGQSIVGCKSVIIQNQVFVIVAQLFGGSHIYKFDEDQSKFTKFQDIEVSKISKPNDIEAFQIGDDWFFVIADSSKAGLSTLYKWNDKGFYSYQSLHEWYRDTDAEFVDLDGKAHLILASRSQVPVIYQWSKSVQKFVLQGEIPNMEDVVAVKAFWIKEEIYLAMTRYIGDSKVLHWTAKHFSEVQALPSRGSMILQPFTFKDRYYLALGSDYTFSQIYLWDAEKKTFERFKEVYIQAPRSFTVVSTDRRDFIFASSFKGSTQIFEHIIIDLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CUM5</accession>
    <name>A0A3B4CUM5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TMED2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CUM5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014224"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022092.2">
        <property type="protein sequence ID" value="ENSPNAP00000014224.1"/>
        <property type="gene ID" value="ENSPNAG00000003636.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155143"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017334827" description="GOLD domain-containing protein" evidence="12">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="13">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="208" mass="23442" checksum="4BA8C2DBC3E34CE9" modified="2018-12-05" version="1">MFTFSELMVLLATLSATASGYFVSVDAHAEECFYERVNSGTKMSLMFEVAEGGFLDIDVEITGPDGKQIYKGDRESSGKYSVAAHMDGTYKFCFSNKMSTMTPKIVMFTIDIGEAPKGDGMETEVGGGTWDAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D098</accession>
    <name>A0A3B4D098_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BEND7</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017576433.1">
        <property type="nucleotide sequence ID" value="XM_017720944.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D098"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016983"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025654.2">
        <property type="protein sequence ID" value="ENSPNAP00000016983.1"/>
        <property type="gene ID" value="ENSPNAG00000023186.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441405"/>
    <dbReference type="CTD" id="222389"/>
    <dbReference type="GeneTree" id="ENSGT00940000163804"/>
    <dbReference type="OrthoDB" id="9944532at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053072">
        <property type="entry name" value="BEN_domain_protein_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35068">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35068:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEN" evidence="2">
        <location>
            <begin position="290"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="447" mass="50800" checksum="9D34FCE821334E85" modified="2018-12-05" version="1">MEFRERRRSRKSQSFKLISEQDYGPVFDSPEHNDTDSEPQDTSENEAWLGEEGMEIKRQITGMMRLLNDKAGRAYQRVEREGEAVKEEPQEEALSFVLPQNQTLEEPQHCWSPGAPMSQSQYDTRSKTQKMLNNSKSKNLARPPVPAAMPNESSCCMCNCKSTLQAILLELRTMRKLMQTQRGNHEKQCTVGNTCSTYFSLPRKRPRKRRAVLRVTSLTPTTKTATPIYLPHVAEPLEETCQKEKPLSEMAMPSSPTTPNISFPNLKPSAQYSLNRGQNCTEPEVQLAEDYEVFIPKAQLDSILLNYTRSGSLLFRKLVCAFFDDATLANSLPNGKRKRGLNDHRKGLDQNIVGAIKVFTEKYCTANRIEKLPGPRDWVQILQDQIKLARRRLKRAETSTDCDEILNRTRTDTTEELQLTPENGENGMSLNGAVYVQKSCETAEENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0C0</accession>
    <name>A0A3B4D0C0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Thrombospondin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Glycoprotein G</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the thrombospondin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0C0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017767"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026678.2">
        <property type="protein sequence ID" value="ENSPNAP00000017767.2"/>
        <property type="gene ID" value="ENSPNAG00000024159.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155832"/>
    <dbReference type="OrthoDB" id="14563at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000004">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000004">
        <property type="entry name" value="Cartilage oligomeric matrix protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000007">
        <property type="entry name" value="Thrombospondin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000009">
        <property type="entry name" value="Thrombospondin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000070">
        <property type="entry name" value="Thrombospondin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000025">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000027">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000001">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000002">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000041">
        <property type="entry name" value="thrombospondin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017897">
        <property type="entry name" value="Thrombospondin_3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008859">
        <property type="entry name" value="Thrombospondin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028974">
        <property type="entry name" value="TSP_type-3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199">
        <property type="entry name" value="THROMBOSPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199:SF78">
        <property type="entry name" value="THROMBOSPONDIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02412">
        <property type="entry name" value="TSP_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05735">
        <property type="entry name" value="TSP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103647">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51234">
        <property type="entry name" value="TSP3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51236">
        <property type="entry name" value="TSP_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 21" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043725027" description="Thrombospondin-1" evidence="24">
        <location>
            <begin position="19"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="26">
        <location>
            <begin position="312"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="25">
        <location>
            <begin position="544"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="25">
        <location>
            <begin position="643"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="repeat" description="TSP type-3" evidence="22">
        <location>
            <begin position="724"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="repeat" description="TSP type-3" evidence="22">
        <location>
            <begin position="783"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="repeat" description="TSP type-3" evidence="22">
        <location>
            <begin position="880"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="repeat" description="TSP type-3" evidence="22">
        <location>
            <begin position="916"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="TSP C-terminal" evidence="27">
        <location>
            <begin position="955"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="721"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="773"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="837"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="735"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="837"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="880"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="891"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00077057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017767.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1172" mass="129930" checksum="99CA28B8B4F8815D" modified="2024-11-27" version="2">EMKLTGIFLLVMLWSCEGARVAESRDDNSVYDLFELVQVPKKNHGVTMVKGDDPYSPAYKILNPDLIPPVPESAFRDLMYSVQAERGFLLLVNFKQFRRTRGALLTVEKLDGSGPVFEIVSNGKANTLDVVFSTENKQQVVSIEDADLATGHWKNITLFVQDDRVQFYVGCEEINIAELDASIQNILTPDTPGVANLRIGKGAVNDKFMGVLQNVRFVFGTTLEAILRNKGSIMTDMITLDNPVNGSRPAIRTDYTGHKTKDLQMICGFSCDDLASMFKELKGLGVVVQELSNELRKVMNLSLAYSMGIYGGVCLHNGIVHKDKEEWTVDDCTECTCQNSATVCRKISCPLIPCANATVPDGECCPRCGTPSDSAEDGWSPWSEWTHCSVSCGRGIQQRGRSCDRINNNCEGTSVQTRDCYLQECDKRFKQDGNWSHWSPWSSCSVTCGSGIITRIRLCNSPTPQLGGKDCQGQGRQTEKCEKSPCPINGGWGPWSPWDACSTTCGGGVQNRKRLCNNPPPKHGGKDCVGDATATQLCNKQACPVDGCLSNPCFAGTQCTSFPDGSWKCGKCPAGYTGNGINCKDINECKEVPDACFQFNGVHRCENTEPGYNCLPCPSRYSGPQPFGQGVEDAAAKKQVCTPRNPCLDGTHDCNKNARCNYLGHFTDPMYRCECKPGYAGNGHICGEDTDLDGWPNADLVCVENATYHCKKDNCPNLPNSGQEDYDKDGIGDACDNDDDNDDIPDDRDNCPFIYNPRQYDYDRDEVGDRCDNCPYNSNPDQTDTDSNGEGDACAVDIDGDGILNEKDNCPYLYNVDQRDTDLDGVGDQCDNCPLEHNPDQLDTDSDRVGDKCDSNQDIDEDGHQNNLDNCPYIPNANQADHDKDGKGDACDYDDDNDGIPDDKDNCRLAFNPDQLDSDGDGRGDACKDDFDQDNVPDIYDVCPENFDISETDFRKFQMVPLDPKGTSQIDPNWVVRHQGKELVQTVNCDPGIAVGFDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQTYWSSTPTKAQGYSGLSIKVVNSTTGPGEHLRNALWHTGNTPGQVHTLWHDPKNVGWKDFTAYRWHLTHRPRTGHIRVVMYEGKKIMADSGRIYDKTYAGGRLGLFVFSQEMVYFSDLKYECRGMVHYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0G3</accession>
    <name>A0A3B4D0G3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KRTAP4-2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0G3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025767.2">
        <property type="protein sequence ID" value="ENSPNAP00000017085.2"/>
        <property type="gene ID" value="ENSPNAG00000023292.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000241985"/>
    <dbReference type="OMA" id="SMGCRSM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017085.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="157" mass="17832" checksum="2B57637150F6783A" modified="2024-11-27" version="2">MCSCCVYFPVLSMCSCCVYFPVLSMPTYRRGRCCRPTCPRGRCCRPTYRRGRCCRPTCRRGRCCRPTCPHGRCCGPTCPRGRCCRPTCPRGRCCRPACLRGRCCRPTCPRGRCCRPACLRGRCCRPTCPRGRCCRPACLRGCCCRPACLRGRCCLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2A2</accession>
    <name>A0A3B4D2A2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spermatogenesis-associated protein 2 PUB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SPATA2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553720.1">
        <property type="nucleotide sequence ID" value="XM_017698231.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017553721.1">
        <property type="nucleotide sequence ID" value="XM_017698232.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397227.1">
        <property type="nucleotide sequence ID" value="XM_037541330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2A2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017206"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025927.2">
        <property type="protein sequence ID" value="ENSPNAP00000017206.1"/>
        <property type="gene ID" value="ENSPNAG00000023425.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427767"/>
    <dbReference type="CTD" id="9825"/>
    <dbReference type="GeneTree" id="ENSGT00530000063956"/>
    <dbReference type="OMA" id="NKQRPIN"/>
    <dbReference type="OrthoDB" id="9989817at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990108">
        <property type="term" value="P:protein linear deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060544">
        <property type="term" value="P:regulation of necroptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010803">
        <property type="term" value="P:regulation of tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048839">
        <property type="entry name" value="SPATA2_PUB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15326:SF8">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15326">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 2/TAMOZHENNIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21388">
        <property type="entry name" value="SPATA2_PUB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 2 PUB-like" evidence="3">
        <location>
            <begin position="9"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="545" mass="60132" checksum="83CFDD23BCD0328F" modified="2018-12-05" version="1">MDAKLREDLFRRYVASLEKRLEEGAGGEIVGGGGQGTTSEEALISTATALLGAYQPDPGHRFRMIRFYDIVENSLRTLKGANLRTLETAFATLETVCTNLLLFPWKKEFRCIKTFTGPYVYHLQSAICDADLRALLRSMGYSRDQELQYIVREHPGGSAHLRQLAFELLLAQAECRLLGEVVALSRGTASELEAVEVRRNTREDAAGCADALRRRDSLTGDMTRLTVRPVDIERAHLRRSGRPSKSVDVTDSAGHWHQASKPILKASLSLRKEPLFVDTEEDLKDEIIRPGTSLHPAAALPSHSPVADFFPIQSPPSEPYPYHLSSLDEIDLYTERGLGGRQTPSRPPSREARDGWAIKSHGVKCQGCGLGCPTLSSCQRCDMILCPSCHAVEPTPCCGFQDYPKTSRPLDGYMPVKEKLSVYSSSAHSHTHPHPHPHPLPHSHSHSPLLDKPMISTKLFPSKPLTLSAPSPTGGERLNSSGSRCGFCNKPGASHTCINCSKVSCDSCMSLYASDMCTRKSLHHSFVPNHQLNYKSSTISHLVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2D9</accession>
    <name>A0A3B4D2D9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="20">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">FAR2</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of saturated and unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols. It plays an essential role in the production of ether lipids/plasmalogens which synthesis requires fatty alcohols. In parallel, it is also required for wax monoesters production since fatty alcohols also constitute a substrate for their synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z)-octadecenoyl-CoA + 2 NADPH + 2 H(+) = (9Z)-octadecen-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36323"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:73504"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(9Z,12Z)-octadecadienoyl-CoA + 2 NADPH + 2 H(+) = (9Z,12Z)-octadecadien-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36363"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:73534"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:36364"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>16-methylheptadecanoyl-CoA + 2 NADPH + 2 H(+) = 16-methylheptadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:81763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84911"/>
            <dbReference type="ChEBI" id="CHEBI:231998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:81764"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>18-methylnonadecanoyl-CoA + 2 NADPH + 2 H(+) = 18-methylnonadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:81767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84914"/>
            <dbReference type="ChEBI" id="CHEBI:231999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:81768"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:52717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>eicosanoyl-CoA + 2 NADPH + 2 H(+) = eicosan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:81727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:75627"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:81728"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hexadecanoyl-CoA + 2 NADPH + 2 H(+) = hexadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36315"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16125"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:36316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>octadecanoyl-CoA + 2 NADPH + 2 H(+) = octadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36319"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32154"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:36320"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2D9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017725"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026627.2">
        <property type="protein sequence ID" value="ENSPNAP00000017725.2"/>
        <property type="gene ID" value="ENSPNAG00000024058.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006367"/>
    <dbReference type="OMA" id="YFTTNGW"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000123">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF120">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 20" id="KW-0521">NADP</keyword>
    <keyword evidence="7 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="476"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="500"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="22">
        <location>
            <begin position="15"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="21">
        <location>
            <begin position="365"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017725.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="523" mass="59916" checksum="24D5DE98FB4297F8" modified="2024-11-27" version="2">MACISEWYAGKNVLITGATGFMGKVLVEKLLRSCPEVKALYLLVRPKAGQSMQERVQDMLKCKLFDRVREDNPEFHQKIVPISSELTQPGLALSSDDVKKLTDCINIVFHCAATIRFDEPLKHALQLNVIATQQLLTLALQMQHLHAFIHISTAYANCNRRHIDEVIYPPPVEPKKLIDSLEWMDDGIVRDITPRLIGDRPNTYTYTKALAECVVQQESGKLNVGIIRPSIVGASWQEPFPGWIDNFNGPSGVFIAAGKGILRTMRASNDAVADLIPVDVVINLTLAAGWYTAVHSPKSALVYNCTTGGINPFHWGEIGTLFLSSSEHHVMSTFKRNPLEQAFRRPNANITSNYLINQYWILVSHKFPALLYDLFLRLSGQKPQMMRIFNRLHKAIGLLEYFSSQDWDWNSDNMNMLLNQLSPEDRKTFNFDVRQLNWPEYIENYCIGTKKYVLNEDMSDIPAARQHLRKLRNIRYTFNTLLLVFIWRVFIARSQMARNIWYFVVSLCFKFLSYFRASSTLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D378</accession>
    <name>A0A3B4D378_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KPNA3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D378"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028204.2">
        <property type="protein sequence ID" value="ENSPNAP00000018797.2"/>
        <property type="gene ID" value="ENSPNAG00000025361.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244891"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000004">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000009">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="14">
        <location>
            <begin position="29"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="138"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="308"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018797.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="545" mass="60093" checksum="9BFC29AF6E8EC922" modified="2024-11-27" version="2">PHHVLSRLAAAPPPPLLASFPVLLSTSPPQTRTAMAENAGLENHRIKSFKNKGRDVETMRRHRNEVTVELRKNKRDEHLLKKRNVPQDESLEDSDVDADFKGNATSDNAVVQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVKCLERDDNPSLQFEAAWALTNIASGTSAQTQAVVKSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFINPSIPITFLRNVTWVIVNLCRNKDPPPPMETVQEILPALCVLIYHTDINILVDTVWALSYLTDGGNEQIQMVIDSGVVPFLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDVLSHFPNLLTHPKEKINKEAVWFLSNITAGNQQQVQAVIDAGLIPMIIHQLAKGDFGTQKEAAWAISNLTISGRKDQVSYLVQQNVIPPFCNLLSVKDSQVVQVVLDGLKNILIMAGEEASTIAEIIEECGGLEKIENLQQHENEDIYKLAFEIIDQYFSGDDIDEDPSLIPDSTQGGTFNFDPASNLQTKEFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8R9</accession>
    <name>A0A3B4D8R9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">HtrA serine peptidase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Serine protease with a variety of targets, including extracellular matrix proteins and proteoglycans. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. Consequently, may regulate many physiological processes. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms homotrimers. In the presence of substrate, may form higher-order multimers in a PDZ-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566042.1">
        <property type="nucleotide sequence ID" value="XM_017710553.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8R9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019828"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029917.2">
        <property type="protein sequence ID" value="ENSPNAP00000019828.2"/>
        <property type="gene ID" value="ENSPNAG00000026598.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435038"/>
    <dbReference type="CTD" id="565082"/>
    <dbReference type="GeneTree" id="ENSGT00940000156955"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="cpPDZ_HtrA-like"/>
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        <property type="entry name" value="Kazal_dom"/>
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        <property type="entry name" value="Peptidase_S1C"/>
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    <keyword evidence="5" id="KW-0964">Secreted</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
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            <begin position="21"/>
            <end position="471"/>
        </location>
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            <end position="103"/>
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            <begin position="402"/>
            <end position="468"/>
        </location>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="471" mass="50171" checksum="83AFAB184577FA4C" modified="2024-11-27" version="2">MMMLVLATCALLCLSPALSAVRLCPDRCDKSACPPVPAECLAGDTLDRCSCCPVCASGEGEPCANARDCADGLSCSLPEGARPTTATVRRRARPGGVGVCTCPSGEPVCGSDGVSYRNICELKSVSARATELQQPPIIFIQRGACGTGHEENPDSPRHKYNFIADVVEKVAPAVVHIELYRKMVFTRREVAVASGSGFVVSEDGLIVTNAHVVANKHRVKVELKTGATYDAKIKDVDEKADIALIKIDAPMKLPVLLLGRSADLRPGEFVVAIGSPFSLQNTVTTGIVSTAQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIRQFLTESHDRQAKGKLPTKKKYIGVRMMTLTSTLAKELKERQSDFPDVTSGAYVIEVIPKTPAEVGGLQESDVIISINGQRIGSASDVSAAIKKDETLRAVVRRGNEDVILTIIPEEIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAS6</accession>
    <name>A0A3B4DAS6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Chromosome 16"/>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020548.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8787" checksum="E4E44278B18FBDDC" modified="2024-11-27" version="2">MVLSLKIISWNIRGIVSPAKRTKVLNHLNTLKSDICFIQETHLTEAASRNLTINNGLARLITLAYCFKLSILLSLHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFM4</accession>
    <name>A0A3B4DFM4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BNC1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFM4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040842.2">
        <property type="protein sequence ID" value="ENSPNAP00000021876.1"/>
        <property type="gene ID" value="ENSPNAG00000029116.2"/>
    </dbReference>
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    <dbReference type="OMA" id="MEPRVPF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040436">
        <property type="entry name" value="Disconnected-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15021">
        <property type="entry name" value="DISCONNECTED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15021:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN BASONUCLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="315"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="657"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="838"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="360"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="487"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="787"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="879"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="788"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="801"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="902" mass="99899" checksum="146923DE04722F11" modified="2018-12-05" version="1">MLNCSCDSFSPGKLRRRQCENCRHGWVAHALSKLKVHHMYQGSQVEIVHSNVVFDICSLMLYGTQAIPVRLKILLDRLFSVLKQDEVIQILSALDWTLQDYIRGYVLQDVAGKVLDRWAIMTFEEEIATLQQFLRFGETKSIVELMALQDKEGQAVIVPTARANSDIRSFIESSMQRPTAPPPKSEPLSRSNGHHFESLVNSMAFMLPLQLLNSAPAPLLSSRADSSLQEAPVRHETLELSPDNSTHFISDPEREEVHLDRVSTKVESDDFTISDNYSSPSTPCTPSISSDITQMSPESKIRPGEKSTVMKKGRVICSACEKTFYDKGTLKIHYNAVHLKIKHKCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMNRNNRDKDLRGGLSEGEANSPQAEKREFVAATGSADSSRSVSGYLSSEPKLHSSFPSVSHSGILFPNLKTVQPVLPFYRSLVTPAELANTPGNLPSLPLLSSSVSVSVGELGNNTECVPKKKSRKSSMPIKIEKDELAEGSGSDEDESLPASTDAGMEYVIQTECDEHSIVPQTIQTQRSHTEGNQSEAKYHQNESCIMGCPSPFSGSQRELHCPSDWNRLLQQPEDQTCSEAGCGENELKKTPCKTEDAEISRNDLRAVCADGGEDLQIVTCEDNVEDDLHQCESCNKTFKNPYSVKMHYRSVHLKEMHMCTVAGCNAAFPSRRSRDRHSANLNLHHKLLTKDHYSTSGAAFTMSKDTSSDITHKETLSQASVIFKGSNRMGLVFPMSKAVENVEGPTKDMLEDETVLDLSTRGSGQSPSWDSDAGSEEGLPLEDSDESCDGLTAGQAASPAAQQLHSSSPITCHVCQKVYSNKGTFRAHYKTVHLRLLHKCKIPGCDTTFSSVRSRNRHSQNPNLHRNLPVLNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFQ8</accession>
    <name>A0A3B4DFQ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LARP4B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017565933.1">
        <property type="nucleotide sequence ID" value="XM_017710444.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFQ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021744"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033317.2">
        <property type="protein sequence ID" value="ENSPNAP00000021744.2"/>
        <property type="gene ID" value="ENSPNAG00000028956.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434967"/>
    <dbReference type="CTD" id="23185"/>
    <dbReference type="GeneTree" id="ENSGT00940000157755"/>
    <dbReference type="OMA" id="HRCRETR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08036">
        <property type="entry name" value="LARP_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058699">
        <property type="entry name" value="RRM_LARP4/4B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF43">
        <property type="entry name" value="LA-RELATED PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26088">
        <property type="entry name" value="RRM_LARP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="5">
        <location>
            <begin position="138"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="393"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="610"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="393"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="481"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="642"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="670"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="709"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021744.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="722" mass="79610" checksum="9584FA3B5D4BA98D" modified="2024-11-27" version="2">MTSDQEGKDIAEPQVQPSQEVRKKEIPLLDATKVSDLNPNAKAWGSYMTNQEASGLAYTRSLPSWVKDTSDPTNTVSGGYVTSNDKGKWNEEQQVSVSVELPSAVPPEAVDMGSTVNQLPPGLQQETSMKESDSSDSSRQLEGLREQLKATLEFCLSRENLANDMYLISQMDSDQYVPIVTVANLDQIKKLSTDVDLIADILKTLPLVQVDKCGEKVRPNQNRCIVILREVPEATPVEEVESLFKSEKLPKFVNCEFAYNDNWFITFQSEADAQLAYQYLREEVKTFQGKPIKARIKAKAIAVNTFVPKNGYRPVDMSPNIQQRYTSYYIPPVYGTQQQFPPIYRLVTPQGWSTTQSYLDPLVTPFPNPGFINGFAGSPTFKSATAPLTVRHYQPRNRNHSKSHVRPSAPTAERGTALLENPGTFTNLSSERIPNGTRSLHTHQISSRPRLPSAPSYPRRDQVGSGRMEVNGADYSPVIGRGRRNGFGYKKRRDDKFTRASTQSPPPAQERTPSPNFELGLSSFPPLPGAAGNLKTEVKMENNLENRLSDIVTGVAKDKALNKDAVTSSVTSGIPKESAQTVIAPTAQPPVEQQNPSSPVHKRCSLKIHEEKSKEVTAPAEKPSVPHAAKSPPVQLNNPVTEPRKPSYAEICQRIREAPMQQPAVEPKPTGSSAEDANSLDSVEHRCRETRLPSAKAALPRVRDAWKSHQGTGETSASSSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGM5</accession>
    <name>A0A3B4DGM5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nuclear receptor subfamily 2, group C, member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NR2C1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552432.2">
        <property type="nucleotide sequence ID" value="XM_017696943.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552433.2">
        <property type="nucleotide sequence ID" value="XM_017696944.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552434.2">
        <property type="nucleotide sequence ID" value="XM_017696945.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552435.2">
        <property type="nucleotide sequence ID" value="XM_017696946.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGM5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022593"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034805.2">
        <property type="protein sequence ID" value="ENSPNAP00000022593.2"/>
        <property type="gene ID" value="ENSPNAG00000029993.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427041"/>
    <dbReference type="GeneTree" id="ENSGT00940000158165"/>
    <dbReference type="OMA" id="EMDRDHR"/>
    <dbReference type="OrthoDB" id="10024684at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06967">
        <property type="entry name" value="NR_DBD_TR2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06952">
        <property type="entry name" value="NR_LBD_TR2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000012">
        <property type="entry name" value="Nuclear receptor subfamily 2 group C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000015">
        <property type="entry name" value="Nuclear receptor subfamily 2, group C, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048245">
        <property type="entry name" value="NR2C1/2-like_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048246">
        <property type="entry name" value="NR2C1/2-like_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="110"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="348"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="287"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022593.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="66273" checksum="3BA05AABFFDBC0FD" modified="2024-11-27" version="2">MAGESPRIQLVSADGGLQIVTDQQLGQKVQIVTAYDQAGSGKQHFILANVDYPSQDKLLLKHESSPGKVILTSADGSAVNQLLFTSPELAGQQIQFVTESTEQSPIKPVVEYCVVCGDKASGRHYGAVSCEGCKGFFKRSIRKNLVYTCRGTGECVINKHHRNRCQYCRLQRCMALGMKQDSVQCERKPVEVSREKPANCAPSIEKIYIRKNLCSPLAAMPTFVNDKETARSANLLDTNMLLNIQQSLSKLDNTIFIPSSPDQNDACQGDLSTLANVVTSLAHMNKSREVTDSSTDLSGMETMSNEDNSMTEVPPDEQNSSEISQAFDTLTKVLQPEEAGGEAVEGSVAEEHSAALLELEGPLLSEMHVPFKLMMPLPMPEFLNLNYICESASRLLFLSMHWARSIPAFQALGPENGISLIKACWNELFALGLAQCAQVMNVETILTAIVSHLQSSLEEEKLSAERVKQVMEHIWRMQEFCNSMSRVNPDAYEYAYLKATVLFSPDYSGVDSTLQIERFQEKAYMELQDYVSRVYPEDTYRLSKLLLRLPALRLMSAAVTEELFFAGLIGNVQIDSIIPYILKMDSTDYNSQSVSAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHJ1</accession>
    <name>A0A3B4DHJ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552393.1">
        <property type="nucleotide sequence ID" value="XM_017696904.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552394.1">
        <property type="nucleotide sequence ID" value="XM_017696905.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397104.1">
        <property type="nucleotide sequence ID" value="XM_037541207.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397108.1">
        <property type="nucleotide sequence ID" value="XM_037541211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHJ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022389"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034560.2">
        <property type="protein sequence ID" value="ENSPNAP00000022389.1"/>
        <property type="gene ID" value="ENSPNAG00000029754.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427023"/>
    <dbReference type="GeneTree" id="ENSGT01150000286901"/>
    <dbReference type="OMA" id="SKITWFF"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015125">
        <property type="term" value="F:bile acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF89">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 1B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="632"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="462"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="748"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="763"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="775" mass="83969" checksum="3CB45045976EDEB5" modified="2018-12-05" version="1">MVDPSEEDNAKMTSQQALCTPDKDTDTLKDPCCSTLKMFIMSLSFAYFSKALCGTYMKSSITQIERRFDLSSSLVGLIDGSFEMGNLLFLAVVSHFGAKLHRPRLIGSGCFLMAIGSAITGLPHFFMGRYKYDTVIQGSLNETNSVAACQEATVQSNYPNIHQESSVNFNTGSDCEKEPGSYMWVYVFLGNALRGIGETPVTPLGISYIDDFAKAENSVFYIACLQTISLLGPMFGFLMGSMCAKLYVDIGFVNVDSVTITPKDARWVGAWWLGFFVSSSILLLAGIPFWFFPRSLPKQAEEQREDPSSPHGYQEHSAHPATPTMKLVDIAKGFLPSLKKLLGTPVYLLLLCGSILNFNSFIGLITFKAKYMEQQFGQSASRANFLIGVLNLPVVAVGIFLGGLVMKRYKLSVVSGAQLSFLTSFSAYLLMLLQFGTKCDNLRMAGLTVSYNGTPQISYDAQTLFSQCNQQCSCSAAEWDPVCSDDGITYISPCLAGCMSSSGHGKNTAFHNCSCVLSPSPVEGSTSVSLGQCPRGPACSRSFTSYMAVSVLSSFINSLGTTPAYMVIIRCISPELKSLALGIQTLVIRTLAGIPAPVYFGALIDSTCLKWGMKKCGGRGACRLYNSDKYRVIFLGLISCLSGSSYFFVIAVIILLRKQFSKEALRALQEANQGRLELHSPQLHCDEVSEHCCSPSQPGGEALVPSERTVETTESYAPHECPASPSTVTPEKPGVIVTERVNGLTGVDGNLESAYGGQQDLETESRDPAKELAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DI93</accession>
    <name>A0A3B4DI93_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DI93"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013343.2">
        <property type="protein sequence ID" value="ENSPNAP00000023163.2"/>
        <property type="gene ID" value="ENSPNAG00000000416.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162978"/>
    <dbReference type="OMA" id="SCIYSIQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023163.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="73" mass="8406" checksum="68E63D73CDC97899" modified="2024-11-27" version="2">MKHSELSASLLTEDHFRCPVCTDVLKDPVSIPCGHSYCKTCIQTYWTKPTLENSYCCPQCRKSFRTRPNSMQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DK62</accession>
    <name>A0A3B4DK62_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-binding region-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RNPC3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037392429.1">
        <property type="nucleotide sequence ID" value="XM_037536532.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392437.1">
        <property type="nucleotide sequence ID" value="XM_037536540.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392443.1">
        <property type="nucleotide sequence ID" value="XM_037536546.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392454.1">
        <property type="nucleotide sequence ID" value="XM_037536557.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392459.1">
        <property type="nucleotide sequence ID" value="XM_037536562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DK62"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023324"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012921.2">
        <property type="protein sequence ID" value="ENSPNAP00000023324.2"/>
        <property type="gene ID" value="ENSPNAG00000000524.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441598"/>
    <dbReference type="GeneTree" id="ENSGT00530000063786"/>
    <dbReference type="OMA" id="AINIRHE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030626">
        <property type="term" value="F:U12 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12238">
        <property type="entry name" value="RRM1_RBM40_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12239">
        <property type="entry name" value="RRM2_RBM40_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000948">
        <property type="entry name" value="RNA-binding region (RNP1, RRM) containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000207">
        <property type="entry name" value="RNA-binding region (RNP1, RRM)-containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034147">
        <property type="entry name" value="RBM40_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045164">
        <property type="entry name" value="RBM41/RNPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16105">
        <property type="entry name" value="RNA-BINDING REGION-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16105:SF0">
        <property type="entry name" value="RNA-BINDING REGION-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="409"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="209"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023324.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="509" mass="56741" checksum="EAD4721B4E047A1D" modified="2024-11-27" version="2">MAEQDEDVVNTKRGKTLIVRHLPAELSREEKEDLLKYFGASSVRALSDKGALKHTAFASFSSETSASRALKRLHQLRILGHTLVVEFAKDQDNVAVLSSPAVTDGLRVADQQDGKQKKENQQPNVPLIDTSIAPTLGLKFQTNPTLKYLYPPPSSAIVTNITHTLLSVPKFYVQVLHLMNKMNLPCPFGPITNPPPVFEMAHGLLPPLMPLPPPFPPENPPLPEGAGESSSEESEYESGDDEDKERMIRLMGLVNQPCKRPLRSKAASKRKKPKLKDLLFTPKPDSHSTPGPALQPSDVFEQPQSLGHKKIEFHISADVSTALEVSVQPREQNTEEEAADAAEQEAGETGFGKIFPSTQANRQEESSEGEEDMPSEFISRRELEKGRLSRDEMKRMSVFKSYEAGEPTCRLYVKNIAKQVEEKDLKYIYGRYINTSSEEERNMFDIVLMKEGRMKGQAFIGLPTEKSAERALRDTNGYVLHDKPLVVQFARSARPKQDAGQSKTGAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQ00</accession>
    <name>A0A3B4DQ00_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein phosphatase Slingshot homolog 1</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">SSH-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 23">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017542396.1">
        <property type="nucleotide sequence ID" value="XM_017686907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQ00"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026447"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005093.2">
        <property type="protein sequence ID" value="ENSPNAP00000026447.1"/>
        <property type="gene ID" value="ENSPNAG00000011753.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414114"/>
    <dbReference type="CTD" id="101885659"/>
    <dbReference type="GeneTree" id="ENSGT00940000156133"/>
    <dbReference type="OMA" id="EVPPCAN"/>
    <dbReference type="OrthoDB" id="5779068at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11652">
        <property type="entry name" value="SSH-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000004">
        <property type="entry name" value="Protein phosphatase Slingshot homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000423">
        <property type="entry name" value="Slingshot protein phosphatase 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043587">
        <property type="entry name" value="Phosphatase_SSH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043588">
        <property type="entry name" value="SSH-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864:SF5">
        <property type="entry name" value="PROTEIN PHOSPHATASE SLINGSHOT HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864">
        <property type="entry name" value="SLINGSHOT PROTEIN PHOSPHATASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23040">
        <property type="entry name" value="PH_SSH1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="20">
        <location>
            <begin position="253"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="312"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="374"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="605"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="682"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="788"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="903"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="606"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="687"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="790"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="906"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1016" mass="111885" checksum="354B3319EF3C6D8D" modified="2018-12-05" version="1">MALVTLQRSPTPSAASTASTSTTNAGDATDFGSDDERRLNQSLSESFLMVKGAALFLQQGSSAQGQKPHPHHKHAGDLPQHLQVMINILRSEDRIKLAVRLESAWSDRVRYMVVVYTSGRQDTEENILLGMDFSNKDSKSCSVGMVLPLWSDTKIHLDGDGGFSVNTAGRTHIFKPVSVQAMWSALQVLHKACEVSRRYNYFPGGMSLTWTGYYESCISSEQSCINEWNAMQDLETMRPDSPTVFNDKPTERERTECLIKTKLRSIMMFQDLENVTSKEIRNELEQHMNCNLKEYKEFIDNEMLLILGQMDKATLIFDHLYLGSEWNASNLEELQETGVGYILNVTREIDNFFPGTFCYHNVRVYDDETTDLLAHWNDTYNFIVKAKKNQSKCLVHCKMGVSRSASTVIAYAMKEYGWSLEKAYNFVKQKRSITRPNDNFMRQLAEYEGILDASKQRHNRLWRPDAECDHPDCQQAAASCCSDPEGMTPGTTPCCEAAARSSPLALELDPANPQNYNYYFRRLSDSALDSDPSTPVRAPPLLDVDRVFIEIEDVERDALLDDETFEGRDSLPLPRFAVPGEGTAAQTCSRGAEPLEELRLRLEFSTVEEEDEEEAQKEQEEMEALAEGGRRGGVGAKEKEEECEESGIDLGTMNHLCNENSNNNNHLGTQNSLAESILKKQVDKPSSLPTPKPSQSTLSDSSGQVLAPSPLRNVPSISVEVPACANVPVIPVVPSLLQACGPCCDCAKCSKAASNPCLDSAILVIQESQSQKLHQGGEDRALRQAAEGNEGLKVRDRAGERSTGIARDGDSTLVSERVTVPSHPPHQEALAHLQRSGLVRRRAERLEKLSGLFQDRSRDHPLKRPDHPYAEPFGAQVDLSTDGTAVPLTNEALLAALGSGGLTPVSSPHGSTLTRSSSSDSIRSVRGKPGLVRQRAQEIEARIRLAGLTVPSRLKRSNSLAKLGSLTFSSEDLCSSACSSDAGTLLLLSLSPEPGHAMSPDWLFSPGGTGLAEPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQ52</accession>
    <name>A0A3B4DQ52_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RTN1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQ52"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025608"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007195.2">
        <property type="protein sequence ID" value="ENSPNAP00000025608.1"/>
        <property type="gene ID" value="ENSPNAG00000010801.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440908"/>
    <dbReference type="CTD" id="323706"/>
    <dbReference type="GeneTree" id="ENSGT00940000155077"/>
    <dbReference type="OrthoDB" id="567788at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071787">
        <property type="term" value="P:endoplasmic reticulum tubular network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2480:FF:000001">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF6">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="737"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="608"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="311"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="434"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="525"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU210713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="795" mass="85775" checksum="9ACBB4CF1E82C904" modified="2018-12-05" version="1">MAGQRAVADSWLGEEEELDGLGSSGPPLGSRNWEDVANSYSAEDGRRGTGVAMETASTGDSMSDLFQKSSPGQDDLYTSLLSTHSSSASNPFGGGAPLFSSEGNRSPPAPAGTDSGIGMTPGDPCDLQTHRTDPYNYMDMSDDLCDLGNLGNLGNLGNLGASRSKQQPTRGYDDEDEDDLDGLQDFKLKKHEKEPEPDPFDLERYLEKNAPLSGGLEGKEDLGSAGGQHAFPYVEDPSDEETAEYRSYRMMGTPQTASPVKITVTTDSRNLATQPQRVSPQGGAMERDSVLSFGQQGLPTVTLSEPEDDSAASSANHSPNHSPTGRESPSDILFQPAGIKSASSVRNTSAPYQDSKGPAKPSSPWDVPGFGGQDRDGSGAESGDSEIEQVAEEPDSPRLDKIQSSKGGFSKSGNPFEQSRDANKATNPFEMPQANKTNASNPFGPSSTAKGAFGQSGNPPLYSLLREEREAELDSDLLIESASEESPKREHGYCPPKPSSAPAPNISSSPISAPRPTESAPSNQKPKEKEGEKEKVSVEKPVEKAKPQPQQHEDKPRPSAMGTSAASPVQRTLEDGGSKSTSSGEMKAAGAKQAELPPFLQNFNKQKVVDLLHWRDVRQSGLVFSSVLLLLFSLTQFSVVSVVAYLALATLSATISFRVYKSVLQAVQKTDEGHPFKAYLEVEIALSPDQISKYVEKVQLYVNHMMKELRRLFLVQDLVDSIKFAVLMWLLTYVGALFNGLTLLILAVVSMFSMPVVYEKYQTQIDQYLGLVRTQVNTIMAKIREKVPGAKRKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQB7</accession>
    <name>A0A3B4DQB7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 18">P2X purinoceptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Receptor for ATP that acts as a ligand-gated ion channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 18">Belongs to the P2X receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570386.1">
        <property type="nucleotide sequence ID" value="XM_017714897.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQB7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025144"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008375.2">
        <property type="protein sequence ID" value="ENSPNAP00000025144.1"/>
        <property type="gene ID" value="ENSPNAG00000010056.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437659"/>
    <dbReference type="CTD" id="5026"/>
    <dbReference type="GeneTree" id="ENSGT01020000230351"/>
    <dbReference type="OMA" id="GIHIEWN"/>
    <dbReference type="OrthoDB" id="494673at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004931">
        <property type="term" value="F:extracellularly ATP-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.490.10:FF:000001">
        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.490.10">
        <property type="entry name" value="atp-gated p2x4 ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027309">
        <property type="entry name" value="P2X_extracellular_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001429">
        <property type="entry name" value="P2X_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053792">
        <property type="entry name" value="P2X_RECEPTOR_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00863">
        <property type="entry name" value="P2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF12">
        <property type="entry name" value="P2X PURINOCEPTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005713">
        <property type="entry name" value="P2X_purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01307">
        <property type="entry name" value="P2XRECEPTOR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01212">
        <property type="entry name" value="P2X_RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="373"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="292"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="17">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="117"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR005713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="472" mass="53660" checksum="EFCEDCB6830257BE" modified="2018-12-05" version="1">MAQSWGSFFYSLLDYKTEKFMIAKNKKVGVIFRLFQLGVIGYLIGWVFLWKKGYQEREEAIQSSVFTKLKGVAFTNSTDLGIQVWGAEDYVIPSQGDRVFFIVTNYLETPNQRLGFCAESQKVPDGLCSSSADCVEGETVIAGHGVKTGRCMNETGTCEIHGWCPVEHSHTPSKPLLGKSENFTIYIKNFIRFPKFDFSKSNVLSTTNKTYLKTCYYNKDHNPYCPIFRIGDVVHWTGHNFQEMAVKGGSIGVVINWSCDLDKDYSKCNPEYSFTRLDASKDSTNSVTSGYNFRFARYYRDADERTYRSLYKVYGIRFDIMVNGQAGKFNIIPTVVNIGSGLALLGAGVFICDMILLYMTSTSSFYRERKFEPIGRNREQRPRDMKHGRHHRHTQDGHHRKEDKSAAEEQPLTPVSSPSAHSPVRQPVAVDSSLKPHSLETREQPTVKLHRNPERKAMSPVWSSTTPRSPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DR31</accession>
    <name>A0A3B4DR31_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">MICOS complex subunit MIC13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MICOS13</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MICOS complex subunit Mic13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567542.1">
        <property type="nucleotide sequence ID" value="XM_017712053.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DR31"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026837"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004116.2">
        <property type="protein sequence ID" value="ENSPNAP00000026837.1"/>
        <property type="gene ID" value="ENSPNAG00000012187.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435915"/>
    <dbReference type="CTD" id="125988"/>
    <dbReference type="GeneTree" id="ENSGT00390000002629"/>
    <dbReference type="OMA" id="GWKYMKD"/>
    <dbReference type="OrthoDB" id="5948578at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026769">
        <property type="entry name" value="Mic13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31816">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31816:SF3">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15884">
        <property type="entry name" value="QIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="111" mass="11503" checksum="3FB0DC5036FA1FD5" modified="2018-12-05" version="1">MAAKILPVVKLATKVGIAGGAVYVAYDSGLLGGSDEGSVALSKAKAAIPPAVEEWTKYFGFELPAAPKIEFSPVDAWNSGVRKSIHALSVAPTAACDYTSQGFEYLKGLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DV27</accession>
    <name>A0A3B4DV27_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Colony stimulating factor 1b (macrophage)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017550201.1">
        <property type="nucleotide sequence ID" value="XM_017694712.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017550202.1">
        <property type="nucleotide sequence ID" value="XM_017694713.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DV27"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026931"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003886.2">
        <property type="protein sequence ID" value="ENSPNAP00000026931.1"/>
        <property type="gene ID" value="ENSPNAG00000012294.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425768"/>
    <dbReference type="CTD" id="100004617"/>
    <dbReference type="GeneTree" id="ENSGT00390000015805"/>
    <dbReference type="OMA" id="VEWNCED"/>
    <dbReference type="OrthoDB" id="8702024at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000056">
        <property type="entry name" value="Colony-stimulating factor 1b (macrophage)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008001">
        <property type="entry name" value="MCSF-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10058">
        <property type="entry name" value="MACROPHAGE COLONY STIMULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10058:SF0">
        <property type="entry name" value="MACROPHAGE COLONY-STIMULATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05337">
        <property type="entry name" value="CSF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017449220" description="Colony stimulating factor 1b (macrophage)" evidence="2">
        <location>
            <begin position="29"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="61176" checksum="8EA8E9E981286879" modified="2018-12-05" version="1">MNTNTPAHKAKIRHLCSFLVLCLHLANGAVPGPCKHSVTKDHLLNLRRLIGNQLQNGCSISYNFTERQSLSDICYIKAAFPHMLDLLNTHFRYERDSDNYNYVSSLKNLIYNIYSQRCIPPINEETEDNPVKFAKLYKTSPRVGLEKAEEVIQMYQNLVTKNDKPVEWNCEDEYVEDSQESTTAHLTQMSGTPECKCSCTKMSSTLLKALPLTRFSLSAPTTISPTRPYQEPVFSYKNVGSRASEPGVKQKATKIIQAQSSPVSPDSESYGTTAGNFPNLIFSTTLDLTDGKVESSPSSVLSSSITSSTKLARIPQKKTLLVLHSATSQPSQRSITPLNSHRSTALLLAKRSLDTKDQGILSDFFSKLLQSWITTATSDSAGIHKKGVQITPRATVYNPFQTLHAEVTAAPSLKIMEDISEYISTPAEQSVDVSTPTHIQTHLHMAASLPENVIKAFSEATDCKDSKCQKDTEDMKAQISRDHVERPLEKDFQLTSSSINTALIITSACLALMLIAALLFCKQQRNIRRLFQRPRGTSTQRSSIYDKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVS3</accession>
    <name>A0A3B4DVS3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ATP10A</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="RefSeq" id="XP_017574280.1">
        <property type="nucleotide sequence ID" value="XM_017718791.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000027540"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002337.2">
        <property type="protein sequence ID" value="ENSPNAP00000027540.1"/>
        <property type="gene ID" value="ENSPNAG00000013054.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440074"/>
    <dbReference type="CTD" id="57194"/>
    <dbReference type="GeneTree" id="ENSGT00940000157895"/>
    <dbReference type="OMA" id="QALRCGR"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000022">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000109">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000023">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF81">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE VA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 17 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1093"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1175"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1204"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1235"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1277"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="47"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="1061"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="609"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1365"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1517"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="609"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1461"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="15">
        <location>
            <position position="420"/>
        </location>
    </feature>
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        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        <location>
            <position position="421"/>
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            <position position="758"/>
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            <name>ATP</name>
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            <name>ATP</name>
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            <name>ATP</name>
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            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
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            <dbReference type="Pfam" id="PF16209"/>
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    <sequence length="1546" mass="174367" checksum="DC7CDAEB22420AA4" modified="2018-12-05" version="1">MAREIGEAGDAKSSTIKKHKRKKTKKESKTRIVHANILYDHAKGGDNPNRHYANNKIKTTKYTLLSFIPKNLFEQFHRFANVYFVFIALLNFVPVVNAFQPELALAPVVFILSVTAIKDLWEDYRRHRSDKEINHLDCLVYSRSERCYVEQYWKEVRVGDFIRLRCNEILPADVLLLSSSDPDRLCHIETATLDGETNLKQRQVVRSFIDLDAEFDPLKYNSVIECEKPNNDLNRFRGYIIHRNGRRDGLYKDNLLLRGCTIRNTEEAVGIVIYAGHETKAMLNNNGPRYKRSKLERQMNVDVFWCVIILLVMCLFSAIGHGLWMYQYGDKRPVFDVLSPEGTDLSPVVSAIYLFLTMIIVFQVLIPISLFVSIEIVKICQVFFIHQDMELYDEETDSHLQCRALNITEDLGQMQYIFSDKTGTLTENKMVFRRCTVAGVEYSHDANARRLAMYQEVDSEEEECLSHGGTLPRRESLCSQHSGKVVLRSQSTKSHRRTGSRAEAKRASILSKHTAFSSPMEKDITPDPQLLDKVNDCGSQMEFMRFHSQLLNQIPPDLCDVFDFFIALTICNTVVVSSPNQPRQKVRRFELKSPVKTIEDFIKRFTPSRLTSGSNSSSSSSLTTNRSSSQRACSSMLSSPSAESTLTKLNEEHFPGQGLQQAFSPVPPNYDQAKASAPDKCELRYEAESPDEAALVYAARAYKCSLVGRLPDQVTVELPHLGKLTFELLHTLGFDSTRKRMSVVVRHPLTEQITVYTKGADSVIMDLVRPPSDDSKGKRQRKILFKTQNYLNLYAADGLRTLCIAKKVLSKEEYAGWLRCHLEAETAIQGREELLFESALRLETDLHLLGATGIEDRLQDGVPETIAALRRAGLQIWVLTGDKQETAINIAYACKLLDPEEEIITLNAESQEACAVLLDESLHYIQAKFLCGSSADPHAAKAFQQDYTALRFTGPPVCSPAFSGPSSPFLVHRLGLVIDGRTLAYALDKSLEDKFLAVARSCRSVLCCRSTPLQKSMVVKLVRNKLKVMTLAIGDGANDVSMIQVADVGVGISGQEGMQAVMASDFALPRFQYLQKLLLVHGHWCYSRLANMILYFFYKNAMFVALIFWYQFYCGFSGSAMIDQWYLIFFNLMFSAFPQLITGTLDKDVSAETLQELPQLYMSGQNSEEYKPYMFWMNMIDAFYQSLICFFIPYFAYADSNVDLFTWGTPITTLALFTILLHLGIETKTWTWMNWTSIAFSIVLFFMVALCYNASCPTCYSPSNPYWTMERLLGDPLFYLMCIITPIAALLPRYFYRACQGSLFPSPLQVGRQLDKLPLEMRRHFLSQSKMKFGSAGSVRPNRPPLFPFCRPFFKDCSLRVQHTHNTTTKRKDKAGQANPVHSKGMEMKDTPLSSKNPENHLMCAQKVESRGGKRGSASPNSETSKSRDHPAGPDGLTESCTLPYPKEPPQAGEISLPSDLGTSLSPGQSSELGWLTSTPLLPQTESVQLISPPSGGPPGVTYKKNQEVLDCSNPSLLIQKGGENPSDSPDYLQEHSKPPFRANIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DWK4</accession>
    <name>A0A3B4DWK4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tripartite motif containing 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TRIM9</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017552099.1">
        <property type="nucleotide sequence ID" value="XM_017696610.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000027873"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001426.2">
        <property type="protein sequence ID" value="ENSPNAP00000027873.1"/>
        <property type="gene ID" value="ENSPNAG00000013466.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426839"/>
    <dbReference type="CTD" id="114088"/>
    <dbReference type="GeneTree" id="ENSGT00940000154071"/>
    <dbReference type="OrthoDB" id="295536at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.40:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000017">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase TRIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B-box zinc-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="185"/>
            <end position="234"/>
        </location>
    </feature>
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        <location>
            <begin position="246"/>
            <end position="288"/>
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        <location>
            <begin position="396"/>
            <end position="454"/>
        </location>
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        <location>
            <begin position="458"/>
            <end position="553"/>
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            <begin position="548"/>
            <end position="733"/>
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            <begin position="96"/>
            <end position="135"/>
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        <location>
            <begin position="120"/>
            <end position="135"/>
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            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSPNAP00000027873.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="746" mass="82746" checksum="5A9EB6ED0C8B2E09" modified="2018-12-05" version="1">MDEMEEELKCPVCGSFFREPIILPCSHNICLACARNILVQTPDAESPQSARASGSAASDYDYLDLDKMSLYSEADSGYGSYGGFASAPTTPCPKSPNGVRVFPPAAPPPQQQQHHLLPPTAAPGAPPPHPHPHPPAMMMMMIPRNACLTCPQCHRSLTLDERGLRGFPKNRVLEAVVERYQQSKAAALRCQLCEKSPREATVLCEQCDVFYCDACRVRCHPPRGPLAKHRLVPPAQGRISRRASPRKTSTCTEHELENLSMYCVQCKTPVCYQCLEEGRHSTHEVKALGAMWKLHKEQLSQALNVLSDRATEAKEFLVQLRNMVQHIQENGVEFEACLVAQCDALIEALNRRKAQLLTRVNKEHEHKLKVVRDQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHMTEGQWGKGTLTPRMTSDFDLTLDSGPLLQTIQQLDFVQMKVPAAPILQLEECCSQTNSATLSWKQPPLSTIAVDGYILELDDGSGGQFREVYVGTETICTVDGLHFNSTYKARVKAFNSSGVGQYSKILVMQTSETGHPPCDIKSLATVAWFTFDPASAHPDIILSNDNLTITCNSYDDRVVMGNTAFSRGLHYWEITIDRYDNHPDPAFGIARGDVLKDVMLGKDDKAWAMYVDNNRSWFMHNNSHTNRTDGGIGKGATVGVLLDFARGILLFLINDEQQGPIAFDSLEGLYYPAISLNRNVQVTLHTGLPIPDFYTPGESDPSGSVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DY34</accession>
    <name>A0A3B4DY34_PYGNA</name>
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        <recommendedName>
            <fullName evidence="4">Cytosolic iron-sulfur assembly component 2B</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">CIAO2B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DY34"/>
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        <property type="gene ID" value="ENSPNAG00000003013.2"/>
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        <property type="component" value="Chromosome 25"/>
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        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
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        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
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        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028378.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7664" checksum="B151540D34377AE3" modified="2024-11-27" version="2">MATLIGLSIKVKLLRSLPDRFKIDVHITPGTHASEDAVNKQLADKERVAAALENSQLLEVVNQCLSTRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZY3</accession>
    <name>A0A3B4DZY3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger protein 750</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ZNF750</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZY3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014826.2">
        <property type="protein sequence ID" value="ENSPNAP00000028569.2"/>
        <property type="gene ID" value="ENSPNAG00000003077.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063870"/>
    <dbReference type="OMA" id="YNPVPIR"/>
    <dbReference type="OrthoDB" id="8933073at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008544">
        <property type="term" value="P:epidermis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039363">
        <property type="entry name" value="ZNF750"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039064">
        <property type="entry name" value="ZNF750_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14678">
        <property type="entry name" value="PROLINE-RICH PROTEIN 35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14678:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 750"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15269">
        <property type="entry name" value="zf-C2H2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger protein 750-like zinc finger" evidence="12">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="100"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="356"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="561"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028569.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="652" mass="72439" checksum="4FACEAE6B388E2CE" modified="2024-11-27" version="2">MSVPTKERKPKKPHYIPRPPGKPFKYHCFQCPFTCNEKSHLFNHMKYNLCRNSLSLLSKKAKSPIPQTAMEVSTETATVETPLGVSLQATGDMKDISKVQQKDVYVTTPKNLQRSNTPEKETKSPTEQTSPDESETEGQHSSATLSSAFSPISAFQEEATSSTYKPERSLPDPYPHVYNPVPIRKPLQSFLADPEIINLECPPYTLSPHLYPVHPSYSPYLLPGNCYNHFPSPAQIPPYILDAQRFHQLLPGVLPVHTHTLPTYPTVDQYYRFCHSTPSLSYSTYHPPDQTHLTSLHYPQMSHSILDVHRGCLGNTRPDGLYNSNPYFDQYMMTQRAMLHQAHMLESHVGVGQESRFGCSAAGSPDRPNAKNHTLKDQHTQNGSGMAVLCSQTGDRNAAAPPEEDGIVQDNSTHHLESSTSRPTEQETTPLNLSKKELIRLTTSAHPKTRESFLDIPLNLSLKPSSSTPHSQTQSQDPLHMEKVFQGAVDTMAPQDQDDTTDEQKHTAAFALCQLAQSSLPAQMQSSETSAQFSKYATTNTDEGLNNDKIDCAQALNNYMDSGATDTSQNKTSTHETAETASDSSDTTKTNIPELPNPESAVDVTCPTTCTENVTVFPSKSKAKTEKGGTQNAKRRRHLARSNHILRKRLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1Z9</accession>
    <name>A0A3B4E1Z9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ZNF664</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1Z9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030642"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018898.2">
        <property type="protein sequence ID" value="ENSPNAP00000030642.2"/>
        <property type="gene ID" value="ENSPNAG00000003478.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163453"/>
    <dbReference type="OMA" id="HEEMDDM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001155">
        <property type="entry name" value="Zinc finger 30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000139">
        <property type="entry name" value="zinc finger protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000189">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000180">
        <property type="entry name" value="Zinc finger protein 689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000286">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF158">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="11"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030642.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="349" mass="40641" checksum="987974ABB69CA3AB" modified="2024-11-27" version="2">MDKATAKEKTHHCPECGKSFRNGATLKTHQRIHKGEKLYDCSECGKRFIQRGHLETHQRIHTGEKPFHCSECGKRFTVKSSLQTHQHIHTGVKPYQCSYCGKSFTKGSSLKIHERVHTGEQLYDCSECGRRFTRQSLLQQHQWVHTGQKPYHCSDCGERFTRQSSLKKHKRSHQGVKPYHCSECGNSFTAQSSLQSHQRIHTGEKPYHCSDCGKSFITQSVFQKHKRIHTGEKPYHCSECGRCFRDRGNLVRHQHLHVGEKPFQCAECWRSFNRQSHLQEHQRVHTGKKPYYCSACGKSFNQHSYFQIHQRVHTGEKPYRCSDCGKNFIYLNTLKKHKCTNRKVTANDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2G3</accession>
    <name>A0A3B4E2G3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAM69 protein-kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570159.1">
        <property type="nucleotide sequence ID" value="XM_017714670.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2G3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029935"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017513.2">
        <property type="protein sequence ID" value="ENSPNAP00000029935.1"/>
        <property type="gene ID" value="ENSPNAG00000016349.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437525"/>
    <dbReference type="CTD" id="492795"/>
    <dbReference type="GeneTree" id="ENSGT00520000055625"/>
    <dbReference type="OMA" id="QVTFETW"/>
    <dbReference type="OrthoDB" id="10035316at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020519">
        <property type="entry name" value="DIPK2A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073:SF6">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073">
        <property type="entry name" value="GH11358P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAM69 protein-kinase" evidence="5">
        <location>
            <begin position="207"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="432" mass="49977" checksum="1138173049D634F0" modified="2018-12-05" version="1">MLRFVSLKFARVYRCGKFLFIVALFVILLMNTHNLFASFQRNELTDRRFIGLNKCPACFGTSWCRKFMNSQVTFETWGRLRFLDFFNVKNVYFGQYGEPREGTRRVVLKRLGSNQELAEIDQKICKRATGRPRCDLIQGMYKTEFARLNGNVRLLTPEVVEGWSDLVHCPSQRLLDRVVRRYAETKDSGSFLLKNLKDTERMQLLMTMAFNPEPLVLQSFPSDEGWPFAKYLGACGRMVAVNYVGEELWSFYNAPWEKRVDLAKQLMDIAEQLTNNDFDFALYLLDVSFDNFAVGPRDGKVIVVDAENVIVADKRLIKQNKPENYDVWYESRSEECDKEACLSFSKDILCSRVTVDHNYYAICQNLLSRAATWRGTTGGLLHDPPADMARDGRLASLLDECTKPQKRFGRFQAAKELREYLTQLTEQANAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2K6</accession>
    <name>A0A3B4E2K6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450, family 24, subfamily A, polypeptide 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CYP24A1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2K6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029566"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016799.2">
        <property type="protein sequence ID" value="ENSPNAP00000029566.2"/>
        <property type="gene ID" value="ENSPNAG00000015742.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182905"/>
    <dbReference type="OMA" id="VLMINTH"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042359">
        <property type="term" value="P:vitamin D metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20645">
        <property type="entry name" value="CYP24A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF125">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 24 SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029566.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="517" mass="58492" checksum="1ECDB50CF8F160E9" modified="2024-11-27" version="2">MRAHIRAAPRIAGLLKQQELAGGLQHSKPTSSACVLDAKDAAAPPAAAAARGCPHSLSSIPGPTNWPLLGSLVDLLRRGGLKRQHEALIDYHKKFGKIFRMKLGSFESVHIGAPCLLEALYRKESSYPQRLEIKPWKAYRDLRDEAYGLLLLEGKDWQRVRSAFQQKLMKPSEVVKLDGKINEVLADFIDRIGKVNVNGKIDDLYFELNKWSFETICFVLYDKRFGLLQETVDEEAMDFITAIKTVRMMSTFGMMMVTPVELHKSLNTRTWQEHTAAWDRIFSTAKLYIDKKLKRHSSGQSEDFLCDIYHNSPLTKKELYAATTELQVGGVETTANSTLWVIFNLSRNPQAQDKLLKEIREVVPSGETPCAEHIKSMPYLKACLKESMRVSPSVPFTSRTLDKDTVLGDYTLPKGTVLMINSQALGSNEEYFDNGKQFRPERWLQDKSSINPFAYVPFGIGKRMCIGRRLAELQIQLALCWLIRDYKIVATDQEPVDAIHSGTLVPSRELPVAFVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2L2</accession>
    <name>A0A3B4E2L2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575272.1">
        <property type="nucleotide sequence ID" value="XM_017719783.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2L2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029928"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017557.2">
        <property type="protein sequence ID" value="ENSPNAP00000029928.1"/>
        <property type="gene ID" value="ENSPNAG00000016334.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440734"/>
    <dbReference type="GeneTree" id="ENSGT00940000160325"/>
    <dbReference type="OMA" id="AHGFQKT"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="102" mass="11490" checksum="AF15976ECE1C894B" modified="2018-12-05" version="1">MADNSTQPQAPSKLNQVQEQVNDVKVILKDNINKVLERGERLDDLIGKTDDLQASADSFQRTSTRVSRKFWWKNMKMMIIIGVIVLIIVVLIILLATGVIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2R0</accession>
    <name>A0A3B4E2R0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Interferon regulatory factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the IRF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560764.1">
        <property type="nucleotide sequence ID" value="XM_017705275.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2R0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029519"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016686.2">
        <property type="protein sequence ID" value="ENSPNAP00000029519.1"/>
        <property type="gene ID" value="ENSPNAG00000015637.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431853"/>
    <dbReference type="CTD" id="792160"/>
    <dbReference type="GeneTree" id="ENSGT00940000156288"/>
    <dbReference type="OMA" id="ANMPVSR"/>
    <dbReference type="OrthoDB" id="6538197at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:immune system process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interferon regulatory factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Interferon_reg_fac_CS"/>
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        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
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        <property type="entry name" value="IRF1/IRF2"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF3">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038196">
        <property type="entry name" value="IFN_RF1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="15">
        <location>
            <begin position="5"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="12">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="12">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="13">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PIRNR" id="PIRNR038196"/>
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        <source>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038196-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="309" mass="35336" checksum="AF7BA5A4707CEB47" modified="2018-12-05" version="1">MPVSRMRMRPWLEDKIDSNSIAGLVWVDKEQKMFSIPWKHAARHGWEMDKDACLFKQWAIHTGKFKPGVTEPDPKTWKANFRCAMNSLPDIEEVKDKSINKGCEAVRVYRMLQVVKNKSKRSPSTADGRRKRKVAKVKTEKMDVEETSTDGPTYKQKEMRTQENTVDSTENLNTATSTYDVPDCSGYEVEIGPDSTNDFTSRFQVSPAHSTDYEEKQAIVEITRQLELDSSQWLQSTSSGKGFLCNEVGMTESFHSPESQWSDNSGEELELRFYTELSSGLPSEDVLSYTDMWNSNQYTNSFPQISCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4Y6</accession>
    <name>A0A3B4E4Y6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L1 transposable element RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4Y6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019092.2">
        <property type="protein sequence ID" value="ENSPNAP00000030758.2"/>
        <property type="gene ID" value="ENSPNAG00000017685.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287143"/>
    <dbReference type="OMA" id="MALENSC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.250.20">
        <property type="entry name" value="L1 transposable element, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030758.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22990" checksum="786BD3B4D4E9305F" modified="2024-11-27" version="2">MLQIRRIWKMLTLKVRLVQIREKIDDLENRSRRNLRIIGIPEGAEGSQPTLFMGTFFAELLGASNFPSSPEIERAHRAPAPKPQPGAQPRPMLVHFLSFQVKEEYLRLSRQQGQLLYKNSRVHIFPDLSADLRNRRNEFKSVKLKLHEAGLKFRMQYPAWLIFSVDGTAHKFDSAAEASSFLNTTVLPALNAVPGTASNLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7G5</accession>
    <name>A0A3B4E7G5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7G5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031189"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020044.2">
        <property type="protein sequence ID" value="ENSPNAP00000031189.2"/>
        <property type="gene ID" value="ENSPNAG00000018416.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234621"/>
    <dbReference type="OMA" id="YRSFRHT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15968">
        <property type="entry name" value="7tmA_P2Y6_P2Y3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF36">
        <property type="entry name" value="P2Y PURINOCEPTOR 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="57"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="330"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031189.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="360" mass="41546" checksum="D270A5B980AB1896" modified="2024-11-27" version="2">MVTDVPTALSQRGQGGDDLLLSSSNSSITTSCNIDESYKYIFLPICYSFTFVFSITLNSVVLYRSFRHTKHWNASLIYMVNLATTDFMYGLSLPFLVASYVMRDNWVFGDFMCRLVRFLFYFNLYCSIFFLTCISVHRYLGICHPMRTITLETKRAVKGTCVLVWVAVFALTCPIFRFAVEGFQNCWDDAIDKEFPDYVPYGITLHLLGFFLPFSIIAWCYSRVVMTIFRTLRRKSIKTIITITLLFALCFFPFHVTRTIFLVLKVTSRVQCHTMQMVSICYKITRPLASFNAWLNALLYFLTKEKSAPCCHKPQPSQHGLLWPLRMLGKGGNGEDEAEEAGNHKDNEFKEENNIFKDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8I5</accession>
    <name>A0A3B4E8I5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="16">H6 family homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HMX2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcription factor involved in specification of neuronal cell types and which is required for inner ear and hypothalamus development. Binds to the 5'-CAAGTG-3' core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HMX homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8I5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031591"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020718.2">
        <property type="protein sequence ID" value="ENSPNAP00000031591.2"/>
        <property type="gene ID" value="ENSPNAG00000019047.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160392"/>
    <dbReference type="OMA" id="CWATSAM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000053">
        <property type="entry name" value="H6 family homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051300">
        <property type="entry name" value="HMX_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110:SF4">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="52"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="54"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031591.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="17074" checksum="B556A45E71F1244F" modified="2024-11-27" version="2">MNQQSTHVYFIDSIQPGVEEKQRMPLGTSDQEGPAISPVHKRSGAEAERCSPAKKKTRTVFSRSQVFQLESAFDTKRYLSSSERACLASSLHLTETQVKTWFQNRRNKWKRQLTGNFSLILFFSLSRHKMMQYPSIFMCSQFLWSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E925</accession>
    <name>A0A3B4E925_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major intrinsically disordered Notch2-binding receptor 1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MINAR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E925"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021874.2">
        <property type="protein sequence ID" value="ENSPNAP00000032193.2"/>
        <property type="gene ID" value="ENSPNAG00000020000.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063851"/>
    <dbReference type="OMA" id="EAMEDRK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039706">
        <property type="entry name" value="MINAR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009626">
        <property type="entry name" value="MINAR1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31530">
        <property type="entry name" value="MAJOR INTRINSICALLY DISORDERED NOTCH2-BINDING RECEPTOR 1 MINAR1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31530:SF4">
        <property type="entry name" value="MAJOR INTRINSICALLY DISORDERED NOTCH2-BINDING RECEPTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06789">
        <property type="entry name" value="MINAR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Major intrinsically disordered Notch2-binding receptor 1-like C-terminal" evidence="7">
        <location>
            <begin position="61"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032193.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="188" mass="21748" checksum="6FD3253CC6519F8C" modified="2024-11-27" version="2">MKGHHIWAKNMDITVLPNNNHPEKFLQLDVGMLPATHGMFQIGAALSGQKQWQNRVYSQVSVQGFDHELEQHITPLTLKPKIKKNPLYMDIRTAEAEENHRTKPSWTIEDYDRHAMHGNLAEYLQEDPRKLNFWLEDLYTPGFDSLLKKKEAELKRNKICKILTSVILSVCVVVIIITVPIVVTQRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E9C6</accession>
    <name>A0A3B4E9C6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017580635.2">
        <property type="nucleotide sequence ID" value="XM_017725146.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E9C6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021275.2">
        <property type="protein sequence ID" value="ENSPNAP00000031889.2"/>
        <property type="gene ID" value="ENSPNAG00000019503.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444152"/>
    <dbReference type="GeneTree" id="ENSGT01030000235294"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="263"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031889.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="439" mass="50061" checksum="2DAAEB67F3B1937B" modified="2024-11-27" version="2">MAHQYDYTTTERFEKKNHKEIEELRQENGILKLEINKLRTQLQDRQNELHNFKDRMGKVVDLKLGSSGVLSEDLTNPCRESELKHMYEKLSKNQWAKVLRQLKTGGPEMTKEKIKEIRKKAEGVIQAVLHQSQHDIQKITGTMLCLTPSSQDASHNSKTTRYFDLAIQNIQMAIYQEKKELYNRDKLQDVPEALYPMIDDCYKTGCLMALHNPPLLLDWGSCGQGLFPPIKTGKFSPVVERDEETNALRKSCAAQAGAQISHGNREIVKLDPIKEREEETDFLQREKPEPDKEREEDVNGALTETSLPVPAAQPQTPTHQRQRSVPEKPDFFSKKEEDNNNPSVVSASQPQPQTTQKHKSDSVPAPQQQPQPPQKHNSDSVKPTPQKQRQQNKNADQVNFSASPSRRETPYRATGAQSSSALARMLSWFTPDSFTSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAS4</accession>
    <name>A0A3B4EAS4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017542852.1">
        <property type="nucleotide sequence ID" value="XM_017687363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EAS4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032336"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022176.2">
        <property type="protein sequence ID" value="ENSPNAP00000032336.1"/>
        <property type="gene ID" value="ENSPNAG00000020212.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414465"/>
    <dbReference type="CTD" id="567512"/>
    <dbReference type="GeneTree" id="ENSGT00940000166236"/>
    <dbReference type="OMA" id="INPWIRY"/>
    <dbReference type="OrthoDB" id="2014092at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046931">
        <property type="term" value="P:pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03158">
        <property type="entry name" value="penumbra_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000007">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF397">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="270" mass="30667" checksum="3C659B02CCB61528" modified="2018-12-05" version="1">MDRDLNLNRAETFSFINPWIRYFLFFFSFLFWVFSLLIVAIGVYAKAQKATDTVRDSFLIDPAIILIVVGVVMFFITFCGCIGALRENIHLLKTFSFSLTLVFLTQLAIAILGFFYSDQTRDALGRFVKKAIVHYRDDLDLQNLMDYIQKEFKCCGWNNYTNWSWNLYFNCSQLNPSTERCAVPFSCCTPVPGESVINTMCGFGVQTQKYLEAAKSIYPVGCADRAVMWIESHLLLVGALALGLALPQIAGIVLSQILIYQIQNEINSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECQ5</accession>
    <name>A0A3B4ECQ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECQ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033620"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038054.2">
        <property type="protein sequence ID" value="ENSPNAP00000033620.2"/>
        <property type="gene ID" value="ENSPNAG00000037696.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163991"/>
    <dbReference type="OMA" id="ISEGWIT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="535"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="567"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="611"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="656"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="691"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="723"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="497"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033620.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="773" mass="86287" checksum="981084CD32D3F2B0" modified="2024-11-27" version="2">DWGDFRNSLLHEIREINNRTDLLPGITLGYHIYDACSEVPMAVKVAFQFANGMEPFFNDTDSCSKSAAAAVPALVGDSTSTTSISMTRILGLFGIPQVSHFASCTCLSDKQQFPAFFRTIPSDHHQAAAIAKVVKYFGWTWIGAVHSDTDYGNFGMASFLKAAQEEGICVEYSESYYRTQPRSKLERVANVIRRSTARVIVTFLHEGEFRFLLDELTRQPPPPIQWIGNDAWLIDPEFLRFNMCAGAIGFGFPRSVIPGLREFLMDLSPAQALKSPLLTEFWESSFSCSLKGSSEGARECDGSEDIRALQNPYTDTSQLRATNLVYKATYAIAHAIHGVICNDTQCDKSAKFTPWQILNQLKRVNFTTKNGFQVSFDSSGDPMAVYDLINWQFKENGGLGFMTVGQYDPSKPRAFLSNIFQVPRSVCSESCPPGTRKAVQKGKPVCCYDCIPCAEGEISNKTDSLNCLRCPPEFWPNIHRDGCLPKPVEFLSWDDTLSIILTVFSIAGAFIAVCVAAVFYKHRTSPIVRANNSELSFLLLFSLTLCFLCSLTFIGQPSEWSCMLRHTVFGITFVLCISCVLMKTVVVLMAFRATLPGSNAMKWFGAPQQRLSVLTFTLIQVLICVIWLTTSPPLPFKNSKHYKERIILECGLGSAIGFWAVLGYIGFLALLCFIFAFLARKLPDNFNEAKFITFSMLIFCAVWVTFIPAYVSSPGKFTVAVEIFAILASSFGLFTCIFAPKCFIIMFRPEQNTKKHIMGKLPASFWIAYPYPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECR9</accession>
    <name>A0A3B4ECR9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">AK8</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546690.1">
        <property type="nucleotide sequence ID" value="XM_017691201.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037387573.1">
        <property type="nucleotide sequence ID" value="XM_037531676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECR9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033493"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024458.2">
        <property type="protein sequence ID" value="ENSPNAP00000033493.1"/>
        <property type="gene ID" value="ENSPNAG00000022159.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423706"/>
    <dbReference type="CTD" id="158067"/>
    <dbReference type="GeneTree" id="ENSGT00940000164784"/>
    <dbReference type="OMA" id="DCIRRGW"/>
    <dbReference type="OrthoDB" id="522106at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd22979">
        <property type="entry name" value="DD_AK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036193">
        <property type="entry name" value="ADK_active_lid_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57774">
        <property type="entry name" value="Microbial and mitochondrial ADK, insert 'zinc finger' domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="482" mass="54474" checksum="156D6EA311F77992" modified="2018-12-05" version="1">MDATVKHLRIPPEMSVYAEKHEIFDLFQTLVRNLIIDKPADPIQYLIGMLKRESIEVPRIMLLGPPASGKRTIAKKLCECIGAIQVTASDILKDDTDLTRAAQEYTKKNKEIPRELWIKLILERLSKVDCVRRGWVLEAIPKTREEALCLQEAGIAPEHVVILQAPDAVLIERSLGKRIDPVTGDVYHVTFMWPESAEVAQRLERPQGLNSEDVISRQLQAYHSEAHALHRTYQSCLKIIDADQPHVDVFAQVLTYVMSRHRSAAPHTPRIILLGPPGSGKSLQARLIAKKYDIVSICCGELLKAVAADESSMGELIKPFLESGRRVPDSMVLQILTERLSRLDCVTRGWVLHGFPQDLEQAEALQESKFSPSRVFFLEMTDDVAVERLTLRAIDPVTGERYHSLYKPAPSPEVHARLRYNPCDSEAEVQRRLKEYCIHASALQAFYPQAVRVNADQDAHTVFECLESRLVNRLPKVLPDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECV7</accession>
    <name>A0A3B4ECV7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECV7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034367"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038496.2">
        <property type="protein sequence ID" value="ENSPNAP00000034367.2"/>
        <property type="gene ID" value="ENSPNAG00000023596.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164983"/>
    <dbReference type="OMA" id="FLLDVYW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF236">
        <property type="entry name" value="PROTOCADHERIN GAMMA-C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="690"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="26"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="133"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="242"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="358"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="455"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="579"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034367.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="865" mass="95203" checksum="AFD9EFE302FDC55A" modified="2024-11-27" version="2">FIMMRKMRRKWWIAGLLQALAVWNIIEAQIRYTIPEELNEGTVVGNIAKDLDLKLSEVFERNLRIASESGKQYFGVDSAKGELVVSERIDRETLCSQSATCLLPLEAIVENPLQLYRVEIEIQDINDNSPVFQNKENILNIAESTAPGARFRLEAAQDPDVGSNSLRTYTLSKNEHFVLDIKTETDGMKVPELVLESSLDREKQPVHKLILTALDGGDPVRSGTTLIKIIVEDINDNEPQFELQTYKASVQESAAAGVTVVTIKATDLDEGINGEIQYYFGVHTPDQVKEVFSVNPDSGEITVIGKLDHEATSSYTFDVCAKDKGQPKLEGHSSVQVLVIDENDNPPEIILTSLPNPVPENASVGTVVALINVLDLDSGENGKVDLKIPPKFPFKLKPSFDNHYSLVTESVLDRESNPEYNIDITATDSGSPTLKTVKTINVKVLDVNDNAPVFSQPSYNVYIKENNVAGASLFSVAALDSDVDKNAMLTYSLVDSKVNQVPASSYFYINSENGTIYSMSSFDYEKLKILDVIVQAKDHGSPSLSSNATVHVFILDQNDNAPAVIYPSAVMGSASHQRMPRSAKAGHLVTKVTAVDADSGHNAWISYRLAEAVDASLFSVNLHTGEVRTKRSVSEQDDSSQRLLIEIKDNGEPVQSTTVTVDVLIEDGFHEPVSDYRKKSAEVSKKSGKITLYLIISLASVSLLCLMTFFILLVKCARGSRGSSSCCIRRSESGYKNPNRNLQIQLNTDGPIKYVEVLGGDMMSQSQSFGSYLSPMSEFSDFTLVKPSSTTDFTDTLNVLDASLPDSTWKFESQHRVPHWRRTGIYSLPLSQQYSDEGCREGRCSLCTGYSAVSSTGIRGFTVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFT1</accession>
    <name>A0A3B4EFT1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:41793"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000034705"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038701.2">
        <property type="protein sequence ID" value="ENSPNAP00000034705.2"/>
        <property type="gene ID" value="ENSPNAG00000003808.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164060"/>
    <dbReference type="OMA" id="IINHCTG"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05274">
        <property type="entry name" value="KR_FAS_SDR_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF8">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="8">
        <location>
            <begin position="2"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="9">
        <location>
            <begin position="885"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="7">
        <location>
            <begin position="1980"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="6">
        <location>
            <begin position="885"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="6">
        <location>
            <begin position="1025"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="6">
        <location>
            <position position="918"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="6">
        <location>
            <position position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034705.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2080" mass="229772" checksum="6C7898DF9FD3C09E" modified="2024-11-27" version="2">FENDIAVVGIGCNFPGGEGLDNFWKVLLEGKNCVVEIPGERFDCAEWYDQDDSKPGKSRTQKAALADGFNEFDDKFFGITDTEVEQMDPQHKILLQCTYRALEDAGIPMEKASGSRTGVFVGLMNRDYELTMANVNPVIINHCTGTGLAMSIAANRISYTFNFTGPSLSIDCACSSSLVALHLACQAMREGDCEMAVFGGVSCIFQPRVFVALSKAKMISPGGTSKPFSNKADGYGRGEGCGVILLKPLKKALEDHDNIWGIICKTAVNQDGHTVTPMTKPSMVQQEELLSRIYSADTYLSDVQYVEAHGTGTPVGDPIEAGSISKVIAKGRPAGLGPLCIGSVKSNIGHTESAAGVAGLIKVLLMMKHETIVPSVFYSEKSSSIDAQALNLKIPTKAEKWVTRASSVRVAGINNFGFGGTNAHAVVRQYMPTKQSKEPVKNLHSFFVASAATVKSLTMTIEDTKQNIEAGNITDLQSLLYTSACRRSHLKHKYRKVFHASSLTDLKEKLMRALNKTSVASKQDPRVVFVFCGNGVTYRGMCRQLLKEEPVFREKMREIEDIFQDYRKISLIEKLESESEQDDDFTVPEIIQPLLFAIQVAIANLLMQWGIRPDAVLGHSVGEVAAAHCSGLLSLEDAVKVIYYRSSLQSRATGGKMLVVSNMSLPNMLKILPSYSGRLCLAAQNSPQSCTISGDADAIDSLHKSLCNSGDGKNLFLRVLDVPVAYHSHMMDSIVADVEKSIGSLQAQNMVTELFSTVSGEKACPTDFVNGKYWARNIREPVAFEQAVKSAKQTKNAIFVEIGPRRALQRNIMETVGNDAVVLSSVQPDKDLETMLTVVSKLFETGVNVDWDHFYKGCEMDPISFPRYQFDNVRRDVLIEATFRDTSSGHPVITQKSKDGYVFSCDLSSGSVSYLHDHKHQGIALIPGAFYVELGLATFMTSVKPRVPLSSLQLSVRFHTPCVLSHNTPDMKVQLNPTENTEEMSFQFKVHSLSANYASGKIESTPRNLPEEQYISLDSVYKRCQSVIHSEEFYKYLSLGGFQYGPVFKNKGDVFYGEELRETYSHVTVPDELLPQLHDYYLHPVILDYLMQLLPVTGTYLYLGRPGFPSQIGSLTVFEPLQKEMVVYLRATCLGTEELNVCGCFADKEGRVLVELKHVTITYLGSGPHVVEEYFYHNSYSIAFEHASFLPRPKSLVFADNVGISRGLQQYLGPSSKYIPSTYTKVLLEHGFEGLLSELKIPNVETNFQEVLFMWGDADVTSQKAENVLDCMATCCEILRTIVAGLKTMGFQNSIRVVTYRSSEDSVNHICPGFVLSGMTRACAAELPDLLFQLIDISSVSPEDIKALAQVLGSYPCSKYPELVVKNGQILKPQISHTPIMAKDSSYQSIHSTPCESFVLQTSDPYRLTGLSAVASDRSVETIQSKNVEVQLSKICVHSSDYFAVSVTDLNFGQTLYWNKHTSQNHKCLALDFSGTVTAVGKNVRELKIGDQVVCCYPIAASSKVVIPATACYKMKRVPFLKDSPCVSYFNLAWEILHCALPKVKQQLKLGIFSTTSNSVLVKVLHLTAVKSGWSVTVGTNANRLLHDFSEMVGIVLLPPYDESVIAEASSVMGVRHIVLVSDNQMKTSVSQLAFRGQNDGVHVKILFTSIVMQKLSNENIDCLPLEESESYFICRIMPVVVLSLDSTGELSDIPLILKPQKLFQNNSVYIVTGGLSGLGFETVKFIAQRGGGNIVILSRSSPSHLMEQEINNIKNQCQTAITWIHCDVSESEHVYQAVAQIEKLLPSNPIKGVFHSAVVLHDGLIESLNKALYEKVMRPKVNGVINLHHATKHCDLDYFVCYSSVSAFLGNASQTNYASANTFMDTFCQYRRNIGLPGQSINWGALNLGLLLNKEHFQRFLEAKGMMVLEVTEIHESLEQCLLINKAQQVVCRFNFKNIRYNVLSQNKSLTMRLSALVEEGLKKANMIDSRLEQSSTMSSPKEYIKSVISETTGVEQDELTDDTPLSALGLDSMLAMTLQNLIFQEKGVNIPLVTLLNPESTLSDIVKICIFSHIIYICFSYSVLRLGMLNTSVTVSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EG27</accession>
    <name>A0A3B4EG27_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017558638.1">
        <property type="nucleotide sequence ID" value="XM_017703149.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000034249"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025876.2">
        <property type="protein sequence ID" value="ENSPNAP00000034249.1"/>
        <property type="gene ID" value="ENSPNAG00000023474.2"/>
    </dbReference>
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    <dbReference type="CTD" id="560875"/>
    <dbReference type="GeneTree" id="ENSGT00940000165827"/>
    <dbReference type="OMA" id="SEWCIIL"/>
    <dbReference type="OrthoDB" id="416585at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000054">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000136">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000007">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000008">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000012">
        <property type="entry name" value="Voltage-dependent T-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005445">
        <property type="entry name" value="VDCC_T_a1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF37">
        <property type="entry name" value="VOLTAGE-DEPENDENT T-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01629">
        <property type="entry name" value="TVDCCALPHA1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="387"/>
            <end position="412"/>
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    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="754"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="792"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="875"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="952"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1256"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1294"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1388"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1489"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
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            <begin position="1569"/>
            <end position="1586"/>
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    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1606"/>
            <end position="1624"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1701"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1790"/>
            <end position="1811"/>
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    </feature>
    <feature type="domain" description="Ion transport" evidence="18">
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            <begin position="94"/>
            <end position="422"/>
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    <feature type="domain" description="Ion transport" evidence="18">
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            <begin position="753"/>
            <end position="981"/>
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            <begin position="1254"/>
            <end position="1523"/>
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            <begin position="1569"/>
            <end position="1822"/>
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            <begin position="473"/>
            <end position="513"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="16">
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            <begin position="2167"/>
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            <begin position="2298"/>
            <end position="2319"/>
        </location>
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        <location>
            <begin position="1516"/>
            <end position="1543"/>
        </location>
    </feature>
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        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
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        <location>
            <begin position="491"/>
            <end position="503"/>
        </location>
    </feature>
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            <begin position="535"/>
            <end position="545"/>
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            <begin position="1078"/>
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            <begin position="2310"/>
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            <dbReference type="ARBA" id="ARBA00004141"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00036634"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Proteomes" id="UP001501920"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGH2</accession>
    <name>A0A3B4EGH2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017551707.1">
        <property type="nucleotide sequence ID" value="XM_017696218.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGH2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034316"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026008.2">
        <property type="protein sequence ID" value="ENSPNAP00000034316.1"/>
        <property type="gene ID" value="ENSPNAG00000023526.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426598"/>
    <dbReference type="CTD" id="8106"/>
    <dbReference type="GeneTree" id="ENSGT00940000154606"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12550">
        <property type="entry name" value="RRM_II_PABPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000311">
        <property type="entry name" value="polyadenylate-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF16">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="285" mass="30957" checksum="B3B71D864A40D3F4" modified="2018-12-05" version="1">MAEFGNGLAEESLLDSDPGHPELEDPGVGDEEPGLEEGEAAIEDPELEAIKARVREMEEEAEKLKELQNEVEKQMNLSPPPAGPVIMSIEEKMEADGRSIYVGNVDYGATAEELEAHFHGCGSVNRVTILCDKFTGHPKGFAYIEFADKESVRTAMALDESLFRGRQIKVGAKRTNRPGISTTDRGFPRARFRSRGGNFSSRARYYSGYTPPRGRGRAFRFQDQWRLTTPPPVAAAPPTVSAASLSLSAPAIHTHPILSVWGGGGGGQGDHRPTAGGIYYSSNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHE5</accession>
    <name>A0A3B4EHE5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">BMP5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017549626.1">
        <property type="nucleotide sequence ID" value="XM_017694137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHE5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038659.2">
        <property type="protein sequence ID" value="ENSPNAP00000034631.1"/>
        <property type="gene ID" value="ENSPNAG00000024063.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425467"/>
    <dbReference type="CTD" id="653"/>
    <dbReference type="GeneTree" id="ENSGT00940000158644"/>
    <dbReference type="OMA" id="NYWLLMD"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19395">
        <property type="entry name" value="TGF_beta_BMP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000003">
        <property type="entry name" value="Bone morphogenetic protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033679">
        <property type="entry name" value="DNRLRE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF139">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017408679" description="TGF-beta family profile domain-containing protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="323"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="96"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="302"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="440" mass="49566" checksum="1797E3CDCD2387A9" modified="2018-12-05" version="1">MKLLGLYGLVWSCLGLVQGARAALADHHVHSSFIYRRLRNHERREIQREILSILGLPHRPRPFSPGKHASSAPLFMLDLYNAMTTEDEEAVVVGEEGKGARGKAAEGARKGAHGPPRQPSPSHSPRLATPHDTNFLNDADMVMSFVNMVERDRDFSHQRRHYKEFRFDLTQIPDGEAVTAAEFRIYKDHSHGKYENATLKVSIYQVIKEYQNRDAETFLLDSKKVRASDGGWLVFDITATSNHWVLNPQQNMGLQLCVETMDGRSINMKSAGIIGRSGPQSKQPFLVAFFKASEVLLRSVRATGGKRKNHSRNKSRSQQKTSQAPRSGDQNTSEQKQACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAHMNATNHAIVQTLVHLMFPDNVPKPCCAPTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELL5</accession>
    <name>A0A3B4ELL5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U5 small nuclear ribonucleoprotein 40 kDa protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">WD repeat-containing protein 57</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SNRNP40</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing as component of the activated spliceosome. Component of the U5 small nuclear ribonucleoprotein (snRNP) complex and the U4/U6-U5 tri-snRNP complex, building blocks of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the pre-catalytic and catalytic spliceosome complexes. Component of the postcatalytic spliceosome P complex. Part of the U5 snRNP complex. Interacts with PRPF8. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of the minor spliceosome, which splices U12-type introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017548479.1">
        <property type="nucleotide sequence ID" value="XM_017692990.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELL5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036159"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030246.2">
        <property type="protein sequence ID" value="ENSPNAP00000036159.1"/>
        <property type="gene ID" value="ENSPNAG00000026769.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424769"/>
    <dbReference type="CTD" id="9410"/>
    <dbReference type="GeneTree" id="ENSGT00940000155058"/>
    <dbReference type="OMA" id="IWDIRPY"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000375">
        <property type="term" value="P:RNA splicing, via transesterification reactions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000229">
        <property type="entry name" value="Small nuclear ribonucleoprotein U5 subunit 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="137"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="179"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="221"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="282"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="347" mass="38201" checksum="E7BD96C0F4BC1F56" modified="2018-12-05" version="1">MIEPKKRGADMALVPAAVKRPRNELVAAAQSQQIATMGPPRSSSLQAPIMLLSGHEGEVYCCKFHPSGATLASAGYDRLILLWSVYGDCENFATLKGHSGAVMELHYNTDGSLLFSASTDKTVCVWDSETGERVKRLKGHTSFVNSCFPARRGPQLACTGSDDGTVKLWDIRKKASVHTFQNTYQVLSVTFNDTSDQIISGGIDNDIKVWDLRQNKLIYSMQGHGDSVTGLSLSSEGSYLLSNSMDNSVRVWDIRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKIAAGSADRFVYVWDTTSRRILYKLPGHAGSVNEVAFHPEEPVVLSGSSDKRLYMGEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELP7</accession>
    <name>A0A3B4ELP7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dehydrogenase/reductase (SDR family) member 7C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548617.1">
        <property type="nucleotide sequence ID" value="XM_017693128.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037400339.1">
        <property type="nucleotide sequence ID" value="XM_037544442.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037400340.1">
        <property type="nucleotide sequence ID" value="XM_037544443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELP7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036106"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030060.2">
        <property type="protein sequence ID" value="ENSPNAP00000036106.1"/>
        <property type="gene ID" value="ENSPNAG00000026628.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424862"/>
    <dbReference type="CTD" id="553684"/>
    <dbReference type="GeneTree" id="ENSGT00940000157100"/>
    <dbReference type="OMA" id="NIMDVNY"/>
    <dbReference type="OrthoDB" id="5307821at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05332">
        <property type="entry name" value="11beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052148">
        <property type="entry name" value="SDR_family_member_7C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44668">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44668:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34419" checksum="FB554C1B0813336E" modified="2018-12-05" version="1">MGVPSVMILPLLVVVLAGFYYIYNEVIQFMSKSIVRNKVVVITDAVSGMGSECARLLHEGGARLVLCGPTWDKLESLSDSLCSAADPSKTFTPKLVLLDFSDIDSMEEVIAEIGECYGCVDVLICNSSMKVKAPVQNLSLEMDKTVMDVNYFGPITLAKGILPMMVARRTGHFVLVNSIQGRLAVPFRSSYAPSKHAVQAFFDCLRAEMEEYGISVSTISHTFINATTQNPTPSNSQPTNALWAYILRKLKTHGVNPNDLASEIVKVVNGRRREVLLTHPIPWAALYIRSLLPSFFFAVVAAGVRDGPMADQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EMQ1</accession>
    <name>A0A3B4EMQ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017555583.1">
        <property type="nucleotide sequence ID" value="XM_017700094.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037398989.1">
        <property type="nucleotide sequence ID" value="XM_037543092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EMQ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037747"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034570.2">
        <property type="protein sequence ID" value="ENSPNAP00000037747.1"/>
        <property type="gene ID" value="ENSPNAG00000029771.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428800"/>
    <dbReference type="CTD" id="553785"/>
    <dbReference type="GeneTree" id="ENSGT00940000157405"/>
    <dbReference type="OMA" id="NVDEDCG"/>
    <dbReference type="OrthoDB" id="28057at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050770">
        <property type="term" value="P:regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:synaptic membrane adhesion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Leucine rich repeat containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF8">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 4C"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
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    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017267798" description="Ig-like domain-containing protein" evidence="15">
        <location>
            <begin position="46"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="355"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="464"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="471"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="488"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="647" mass="72415" checksum="8629A919906B2F8C" modified="2018-12-05" version="1">MLNKMTFSQQQQMMRGPRWNRALSDPLFVLLLALQLLVVAGLVRAQTCPSVCSCSNQFSKVICTRRGLRDVPDGISTNTRYLNLQENFIQVIKVDSFKHLRHLEILQLSKNHIRSIEIGAFNGLASLNTLELFDNRLTTIPNGAFEYLSKLKELWLRNNPIESIPSYAFNRVPSLRRLDLGELKRLSYISEGAFEGLSNLRYLNLGMCNLKEIPNLIPLVRLDELEMSGNQLSVIRPGSFKGLVHLQKLWMMHAQIQTIERNAFDDLQSLVELNLAHNNLTLLPHDLFTPLHHLERVHLHHNPWNCNCDILWLSWWLKEMVPANTSCCARCSTPLSHKGRYIGELDQNYFHCYAPVIVEPPADLNVTEGMAAELKCRASSLTSVSWITPNGSIMTHGAYKVRISVLNDGTLNFTNVTMQDTGTYTCMVSNSAGNTTASATLNVSSTENSSFSYFTTVTVETIEPSHDEGHTTVQQKVSPTPSSGVWETPSAPSSTTTTTAQTPLSTKATDKTYTIPVTALGDGSLNTLDEVMKTTKIIIGCFVAITLMAAVMLIIFYKMRKQHHQQNHHAPTRTIEIINVDEDCVTGGPTMEGHLTLPPLEHEHLNHYNTYKTAYNHASTINSIHSSAHEPLLIRASSKDNVQETQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQA4</accession>
    <name>A0A3B4EQA4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RGS2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_017716819.2"/>
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    <dbReference type="Ensembl" id="ENSPNAT00000033757.2">
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        <property type="gene ID" value="ENSPNAG00000005969.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000157937"/>
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    <dbReference type="OrthoDB" id="196547at2759"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
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        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00615">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0734">Signal transduction inhibitor</keyword>
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        <location>
            <begin position="70"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="198" mass="22990" checksum="DAB8F99ECCCF5B0D" modified="2018-12-05" version="1">MRDALYEKDMSCSACLRNSGGAVEPQRSTSKNWRSRLGIFLKTSPHAPPAKTQRKQYRPTTDEVNQWAQSLDKLLSCKYGTIAFRLFMKSEFCEENIEFWLACEEFRHIKSPTKRAARAKRIYKKFIKSEAPKEINVDFNTKDGIFKSLHCSTQSSFIAAQNKVYGLMENNSYPRFLQSQLYTQLCQLAQGRECKSWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQQ6</accession>
    <name>A0A3B4EQQ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LRCH3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037395875.1">
        <property type="nucleotide sequence ID" value="XM_037539978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EQQ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037476"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040927.2">
        <property type="protein sequence ID" value="ENSPNAP00000037476.2"/>
        <property type="gene ID" value="ENSPNAG00000029583.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433438"/>
    <dbReference type="GeneTree" id="ENSGT00940000158330"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000021">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000007">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF44">
        <property type="entry name" value="LEUCINE RICH REPEATS AND CALPONIN HOMOLOGY DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="655"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="413"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="548"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="769"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="501"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037476.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="791" mass="86792" checksum="B832C4BAF9C0B08B" modified="2024-11-27" version="2">MAASVLLSAESTVPTFAVGNPGAAGAAANGLSAPGPASWNRSLDRALDEAAATGCLNLSGRKLKEFPRSAANHDLTDTTRADLSRNRLSELPVEVCIFVSLENLNLYQNCLRSLPESLINLQALTYLNISRNQLSTLPAHVCSLPLKVLIACNNKLLSLPEELGQLRQLTELDVSCNEIQTLPSQVGQLEALRDLNIRRNHLVRLPPELAELPLVRLDFSCNKVTSIPVCYRNLRHLQSIVLDNNPLQSPPAQICIKGKIHIFKYLNMEACKIAPDLPDYDRRPLGFVSCVDELYPGRPYGALDSGFNSVDSGDKRWSGNEPSDELSDLPLRVAELTKEQRQRRERERERDEHRTGSLLANGTLESELEQIDFIDSCTGEEDEEEVRSRGGEPVSLSSQFMAYIERRITREGSPVKANYPRDARIDDSRRHGSSHNRSVMDTVSTLSSPTHSQRAVSGSGVGLERMRREAQLAAQRYEEERQKARTAQRDAVRSYVKHKSTQSPTKLSPTDSETLYPSRRSTHTDDSALFMSDFEPLMVFEIDIDTNTIKKRPGPSKQSVSGKPADGCVSPSVDNAPSCLLQQGEDRASLSPTALQSPTYPGPAAPPSCRGATQRPESFLFRLSQREEKKRGESRAGRPEPEDLTPTQSPASGPTGEEAELVEQLRKNIESRLKVSLPSDLGAALTDGVVLCHLANHVRPRSVPSIHVPSPAVPKLTMAKCRRNVENFLEACRRIGVPQSQLCLPLHILEEKGLPQVAGTVRALLDLAPPKNSASPSTAPANPSTTSTLTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ER96</accession>
    <name>A0A3B4ER96_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ER96"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041252.2">
        <property type="protein sequence ID" value="ENSPNAP00000037819.2"/>
        <property type="gene ID" value="ENSPNAG00000029869.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037819.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="66" mass="7341" checksum="10D408EC0E8930C1" modified="2024-11-27" version="2">MICGRAKKHFSTPSKSLLLASQIIPNTFTKVWALGCSVHCSEHHQFSTFLFHPSFLASSSVQSYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKT2</accession>
    <name>A0AAR2IKT2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580558.1">
        <property type="nucleotide sequence ID" value="XM_017725069.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKT2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060365.1">
        <property type="protein sequence ID" value="ENSPNAP00000038632.1"/>
        <property type="gene ID" value="ENSPNAG00000033868.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108444098"/>
    <dbReference type="GeneTree" id="ENSGT00950000182945"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000095">
        <property type="entry name" value="Rho-related GTP-binding protein RhoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="193" mass="21860" checksum="C5C7BA1992BC43FC" modified="2024-11-27" version="1">MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIYAFGYMECSAKTKDGVRDVFEMATRAALQARKGKKGTKCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKV5</accession>
    <name>A0AAR2IKV5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKV5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084268.1">
        <property type="protein sequence ID" value="ENSPNAP00000038624.1"/>
        <property type="gene ID" value="ENSPNAG00000035998.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286916"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="300" mass="34289" checksum="934B3F0E9EA8C054" modified="2024-11-27" version="1">MAGGLEPPQIKSTSFRLQRGTCQGCPLSPLLFAICIEPLAIRIRQHETLNPIHLGGVDHHLSLYADDVVIFMSQPELSTPILLDLIKSFGYISGYTINWQKSEFMPLGKNPKPEFLHRLPFKVTDKADNSFKYLGIIITKTLADTFNKNFVPLLVKVEDDFARWSTLPLSLAGRVNLVKMAILPKFLYLFQHIPVLISKKFFNKLNKLVSKFLWANKPVRIRSKILQLPKRVGGLALPNYWAANIHKLLYWTDTPMTNQPAWVKIEMSSSQSSLRAWLLFLFFHLLSSFPFPFLFLSSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IVB4</accession>
    <name>A0AAR2IVB4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037388697.1">
        <property type="nucleotide sequence ID" value="XM_037532800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IVB4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047407.1">
        <property type="protein sequence ID" value="ENSPNAP00000043635.1"/>
        <property type="gene ID" value="ENSPNAG00000037453.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411278"/>
    <dbReference type="GeneTree" id="ENSGT00940000162287"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000096">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF127">
        <property type="entry name" value="BLOODY FINGERS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="16">
        <location>
            <begin position="17"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="504"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="561"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="617"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="243"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="317"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="661" mass="75385" checksum="E0ACD3599E20FD28" modified="2024-11-27" version="1">MNQPLSKSLPACSSSIMVRECFVKACFNKQKTWSATMFHRLPLRDEERLKLWLIALGVDTSTPVEKLKGKVVCEEHFQPCDYYEKMELATRTMRRFLRDSAVPSVGVVQPPGVQDCSPKDEPSPALRLADQQHSTSTTSSCTVLAPRTRSEGRRQKVFSEKENTPTPLSTCSEVEEKSWRKAVKSLQTCHGTNIFKCNEVLVSTQCLLELFQYCWLCQIESCITIEGHERQFSITQDCQSCGHHRQWRSHPTPPDEPVEALNTQHKHPEGAASCPEVDRTLQPGDGDSKEVVVEIVSSDNEGTEQNSEQMLIEKDGSYLSSTEDESSEQEEVEKKRKRKSKKQDSSDEWEPHLDEVAADSDVSVGEDGSLIEDGQSKVVMWCTVCRTDATLSCSLHRHKKVFACAQCGAGDGVETHNFEKLLVHFDDFPSFQKHAEKEHGAKPFRKLCQECGKLILAEPKSSGCKDHVCEHKTKPIVCPECGKRFLTEVGLKRHFSKLHKDADHPCKYCLKLFTNRPSKLEHEQTHPKEKQPYSCPDCPERFDNIYKRNTHLKFHRGPYKYACDTCGKRFRDIIKLKRHKLIHSGEKPFRCQVCDRSFNQIGNLSSHMRLHTGEKPFMCEQCGECFNHNVSLKNHLMRYHYSGDRQCQSDAENRTLNRGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWE7</accession>
    <name>A0AAR2IWE7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZFY</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWE7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043227.1">
        <property type="protein sequence ID" value="ENSPNAP00000042374.1"/>
        <property type="gene ID" value="ENSPNAG00000005981.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154150"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="281"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="480"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="51"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="53"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="672" mass="75040" checksum="16B99B2764AA5EF1" modified="2024-11-27" version="1">MIDTDTDIDTDTDTGIDTDTDTGIDTDTGIDIDIDTDADFDIDTDIDTHIDTDTDIDTDTDTDIDTDTDTDTDIDTDTDIDTDIDTDTDIDTDTDIDTDTDIDTDTDFDTDTYIDTHIDTDTDTDIDTDTDIDTDIDTNADTDVDTDIDIDTDIDTDTDTDIDTDIDIDTDTAIDIDTDTDIDTDIDIDTDIDTDIDIDTDIDTDTDIDTDIDTDIDIDTDIDIDTDIDTDTDIDTDIDTDTDIDTDIDTDVQFWEVISDEHGIDATGSYQGESELQLERINVYYNEATGSKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATEDNECYRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWG0</accession>
    <name>A0AAR2IWG0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC35C1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Putative transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TPT transporter family. SLC35E subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577559.1">
        <property type="nucleotide sequence ID" value="XM_017722070.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWG0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046040.1">
        <property type="protein sequence ID" value="ENSPNAP00000044063.1"/>
        <property type="gene ID" value="ENSPNAG00000030152.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108442165"/>
    <dbReference type="CTD" id="55343"/>
    <dbReference type="GeneTree" id="ENSGT00390000013315"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="9">
        <location>
            <begin position="38"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="364" mass="40177" checksum="BB2332549203ACB0" modified="2024-11-27" version="1">MNRAQLKRSRILRMAMSDRGLLEEEEGRGEPFVFRAVKIAGVVALYWFVSITMVFLNNYLLDSKELDAPLFVTFFQCVVSVCLCWVMYALSTFWPACVDFPSIKVDLKVCREVLPLSVVFISMITFNNLCLKNVGVAFYTVGRSLSTVFNVLFSYVILKQTTSFYALLCCGVILGGFWLGVDQEGAAGSLSWSGVFFGVLASACVSLNAIYTKKVMPVVDGNIWKLSYYNNLNACFLFLPLIFVFGELGSLASFSRLGDLSFWGMMVVGGVFGFAIGYVTGLQIKFTSPLTHNVSGTAKACAQTVLAVAYYATSKSVLWWTSNMMVLGGSFAYTWVKGLEMKKAPPPRDSSETREKLLKGESGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IX18</accession>
    <name>A0AAR2IX18_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hypoxia up-regulated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HYOU1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IX18"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069062.1">
        <property type="protein sequence ID" value="ENSPNAP00000044278.1"/>
        <property type="gene ID" value="ENSPNAG00000000759.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157686"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903298">
        <property type="term" value="P:negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000013">
        <property type="entry name" value="Hypoxia up-regulated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000009">
        <property type="entry name" value="Hypoxia up-regulated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000004">
        <property type="entry name" value="hypoxia up-regulated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000012">
        <property type="entry name" value="Hypoxia up-regulated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="578"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="918"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="617"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="713"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="726"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="934"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="946"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1002" mass="112627" checksum="1005A1608F38E8D0" modified="2024-11-27" version="1">QRAGQWTFLFYSLVRPVTMREKLTLWALFCLALVLLPSPTASVAVMSVDLGSEWMKIAIVKPGVPMEIVLNKESRRKTPVAVCLKENERLFGDSALGVSVKNPKVVYRYLQNLLGKTAENPQVAQYQKHFPEHQIQSDEKRGTVFFKNSEEMQYSPEELLGMVLNYSSGLAQDFAEQPIKDAVITIPAFFNQAERRAVLQAAQMAGLKVLQLINDNTAVALNYGVFRRKDINSTAQNVMFYDMGSGSTTATIVTYQTVKTKEAGTQPQLQIRGVGFDRTLGGFEMELRLRDYLAKIFNEQKKSKKDVRENMRAMAKLLKEAQRVKTVLSANAEHMAQIEGLMDDIDFKAKVNRAEFEALCTDLFDRVPGPVEQALAASEMSLDEIEQVILVGGATRVPRVQEVLLKAVRKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFIVRDAAVFPIQVEFSRETEEEDGVKGIKHNKRILFQRMAPYPQRKVITFNRYTDDFIFHINYGDLSFLSQDDLKMFGSQNLTTVKLSGVGNSFKKHSDAESKGIKAHFNMDESGVLLLDRVESVFETAVEEKEEESTLTKLGNTISSLFGGGSSEPSANVTEPVQDEEEVPPEAGKDQDEAVEKPEESAPEKPETEEKSQGKNSKAESQTEEKKPDKEGTDKDEPQVEKEAEKSDEGKTNGVKEDEKEADKKPKPQKKSKISVDIGVELEVNDVPDPSPEDIEASKKKLQDLTDRDLEKQEREKTLNNLEAFLFETQDKLYQDEYLAVVTEEEKEEISGKLSAASTWMDDEGYTAGTKELKEKLSELKKLCKAMFFRVEERKKWPDRLAALDSMLNHSTIFLKSAKMLPEDNQIFTDVELKTLERVINETMDWKNETVAEQEKLSPTEKPVLLSKDIEAKLSLLDREVNYLLNKAKFAKPKPKPKPKDKNSTSTESNKTSSTDSEKVVPPKDETGVEEAKPAEEPPTEENKKETETEQKVEEETEGTGNYTPVMHLDTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IXI8</accession>
    <name>A0AAR2IXI8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IXI8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086287.1">
        <property type="protein sequence ID" value="ENSPNAP00000042767.1"/>
        <property type="gene ID" value="ENSPNAG00000032136.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163991"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000007">
        <property type="entry name" value="Vomeronasal 2, receptor 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="575"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="607"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="651"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="696"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="731"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="757"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="12">
        <location>
            <begin position="543"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="801" mass="89639" checksum="03AA58960386E241" modified="2024-11-27" version="1">MLCLTGLRPTACKTDTVSCRLWADPHYALLLVLVRDTNTNEKNGKYCYVYFLCPVYLNSMDFRELRYARAMEFAIQEINNRTDLLPGITLGYQIHDSCAAVPMAVKVAFQFANGMEPFYNDTDSCSKSAAAAIPLKSVRFQTAITNNVYFLSYTELQVSHYATCACLSDKRQYPTFFRTIPSDHHQAAALARMVKHFGWTWIGAVRSDTDYGNYGMASFLKAAQEEGICVEYSESYYRTQPRSKLERVANVIRRSTARVIVAFINSSEMRVLLEELGRQPPPPLQWIGSETWITHPEFRKYNMCAGAIGFGVPRSVIPGLRQFLLDLSPEQALKSPLLTEFWESSFSCSLKGSSGGARECDGSEDIRALQNPYTDTSQLRVTNMVYKATYAIAHAIHGVICNDTQCDKIPFTVFQIMQLQILDQLNKVNFTTKNGYQVSFDSNGDPVAVYELINWQIEKDGSVDVVTVGRYDASKPRGQEFSMSRAISWLGGQSEVPRSVCSESCPRGTRKAVQKGKPVCCYDCIPCAEGEISNKTGCISQSMFSIAGAFIAVCVAVVFYKHRTSPIVRANNSELSFLLLFSLTLCFLCSLTFIVQPSEWSCMLRHTAFGITFVLCISCVLGKTMVVLMAFRATLPGSNAMKWFGPPQQRLSVLVFTLIQVLICVLWLTISPPFPFKNLKHYKERIIFECGLGSAIGFWAVLGYIGLLALLCFILAFLARKLPDNFNEAKFITFSMLIFCAVWVTFIPAYVSSPGKFTVAVEIFAILASSFGLIFCIFAPKCFIIMFRPDTYTNCIHSTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZX8</accession>
    <name>A0AAR2IZX8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAM21/CAPZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZX8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044061.1">
        <property type="protein sequence ID" value="ENSPNAP00000045255.1"/>
        <property type="gene ID" value="ENSPNAG00000010675.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153997"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029341">
        <property type="entry name" value="FAM21/CAPZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15255">
        <property type="entry name" value="CAP-ZIP_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM21/CAPZIP" evidence="2">
        <location>
            <begin position="69"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="305" mass="33104" checksum="48A8DE6831C570D2" modified="2024-11-27" version="1">CRRISSLDNEASPVKPSVAELAGKFKGHAHPIPVTEERKHVRSPPCFLQLHNQTDGESEQGKPAVPPHPHKGKLKSSPLIEKLQANLSLTPTTLAPSPKSPETKLQPAPFCPISPCGSLSPTLRPPQQPGEDEVPVSFEQPAEGTPLPNINKSRVRLSFKRRPPTRQHRKSAGEEILVDTHERSTSPCAPHSPDKNGEVFSAPQEMKEEDLQDATVVTDQDTDPQEGHSGGVSQDNGGTKVTQGKEEGEVTVAEGSDKPQPTNPAEIQHEEKPSEEQSPEEMEPVEEGGELLPTEENGELKEKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J3M2</accession>
    <name>A0AAR2J3M2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J3M2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068207.1">
        <property type="protein sequence ID" value="ENSPNAP00000046560.1"/>
        <property type="gene ID" value="ENSPNAG00000014866.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144179"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000207">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000005">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="11">
        <location>
            <begin position="314"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="567"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="572"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="592" mass="66515" checksum="5E7AF423F065738F" modified="2024-11-27" version="1">MVSCSYILVTGGVISGIGKGIIASSVGTILKSCGLHVTAIKIDPYINIDAGTFSPYEHGEVFVLDDGGEVDLDLGNYERFLDIRLTKDNNLTTGKIYQSVITKERRGDYLGKTVQVVPHITDAIQEWVMRQAKVSVDDDGVEPQVCVIELGGTVGDIESMPFIEAFRQFQFKVKRENFCNIHVSLVPQPSTTGEQKTKPTQNSVRELRGLGLSPDLIVCRCSTPLDTAVKEKISMFCHVEPEQVICIHDVSSIYRVPLLLEDQGMVEYFCRRLDLPIEMRPRRMLTKWKEMSDRSDRLLEHTSIALVGKYTKFSDSYASVIKALEHSALAINHKLEVKYIDSADLEQSTQQEEPVKYHEAWQKLCSADGVLVPGGFGVRGTEGKIQAINWARKQKKPFLGVCLGMQLAVCEFARNMFGWQDANSTEFDPETKHPVVIDMPEHNPGQMGGTMRLGKRRTIFKSNSSILRKLYGDADHVDERHRHRFEVNPELKHHFEKKGLHFVGQDTEGQRMEVIELEDHAYFVGVQYHPEFTSRPIKPSPPYFGLLLAAAGKLQSYLLKGCRLSPRDTYSDRSGSSSPDSELSELKFPSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J7S9</accession>
    <name>A0AAR2J7S9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Asparagine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J7S9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072357.1">
        <property type="protein sequence ID" value="ENSPNAP00000048083.1"/>
        <property type="gene ID" value="ENSPNAG00000018432.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234618"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000151">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
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        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
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    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="328"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="74"/>
            <end position="108"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
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            <dbReference type="ARBA" id="ARBA00012816"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50862"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048083.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="630" mass="72116" checksum="A8C925B9EA7BCD50" modified="2024-11-27" version="1">MSAYNEVASEWKHKAGVSNKVASEWKHKAGVSNKVASEWKHKVGVSNKVASEGKHKAGVSNKVASEWKHKVGVSNKVASEGKHKAEELYVSDKQGSDQDGDGTEQNPFKTPLRALLFAGKEPFPTIYVDSQKEDERWAVISKTQMKNTKKLFHKEHVKTDVKDKKEAEDAERREKNLEEAKKIVIENDPSLPEPKTAKIFGLESLREQRVKVFGWVHRLRRQGKNLLFIVLRDGTGFLQCVLTDKLCQCYNGLVLSTESTVALYGTVKPVPEGKQAPGGHELHCDFWELIGLAPAGGADNLLNEESDVDVQLNNRHMMIRGENVSKILRVRSTVTQCFRDHFFSRGYYEITPPTLVQTQVEGGSTLFKMDYFGEYAYLTQSSQLYLETCIPALGDTFCIAQSYRAEQSRTRRHLSEYTHIEAECPFMSYDDLLCRLEDLVCDVVDRILKSPAAALLYDINPDFKPPKRPFKRMNYTDAIVWLKEHDIKKDDGTYYEFGEDIPEAPERLMTDSINEVILLCRFPAEIKSFYMQRCPEDRRLTESVDVLMPNVGEIVGGSMRIWDADELLEGYKREGIDPTPYYWYTDQRKFGTCPHGGYGLGLERFLTWLLNRHHIRDVCLYPRFIQRCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDS5</accession>
    <name>A0AAR2JDS5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16 21">Postsynaptic cell membrane</location>
            <topology evidence="16 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSPNAP00000048379.1"/>
        <property type="gene ID" value="ENSPNAG00000006962.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156253"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06382">
        <property type="entry name" value="PBP1_iGluR_Kainate"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutamate ionotropic receptor kainate type subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000210">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutamate receptor ionotropic, kainate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative glutamate receptor ionotropic kainate 1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="Glu/Gly-bd"/>
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        <property type="entry name" value="Iono_Glu_rcpt_met"/>
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        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
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        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
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    </dbReference>
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        <property type="entry name" value="ANF_receptor"/>
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        <property type="entry name" value="Lig_chan"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBPe"/>
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    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like I"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 21" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 21" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 21" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7 21" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="643"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="824"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="22">
        <location>
            <begin position="437"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="23">
        <location>
            <begin position="447"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="693"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="694"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="742"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="19">
        <location>
            <position position="672"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="19">
        <location>
            <position position="699"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="101"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="754"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="877" mass="98960" checksum="7B8EDB1FE6758E3F" modified="2024-11-27" version="1">MPTQFSDWLISSFFKPHFIIFLPFCSNFAGYTFTVILLFCLLVGGIFETLENEPVSVEELAFKFAVTNINRNRTLMPNTTLTYDIQRINLFDSFEASRRACDQLGLGVAAVFGPSHSSSVSAVQSICNALEVPHIQTRWKHPSVDNKDSFYINLHPDYAPISRAVLEIVQFYKWKTVTVVYEDATGLIRLQELIKAPSRYNIKIKIRQLPVGSKDARPLLKEMKKGKEFCVIFDCSYQTSVEVLKQLLFMGMMTEYYHFFFTTLDLYSLDLEPYRYSGVNMTGFRLLNIDNPEVASVLEKWSMERMQAPQKPESGLSEGMMTTEAALMYDAVYMVAAASQRASQITVSSLQCHRHKPWRFGSRFISMVKEAQWNGLTGQIILNKTDGLRKDFDLDVVSLKEDGMEKIGVWNSNLGLNLTDSVRDKSTNVTDSMANRTLIVTTILENPYVMYKKSDKPLYGNDRFEGYCLDLLKELSNILGFSYEVKLVSDGKYGAQNDKGEWNGMVRELIDHVADLAVAPLTITYVREKVIDFSKPFMTLGISILYHKPNGTNPGVFSFLNPLSPDIWMYVLLACLGVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLINSIWFGVGALMQQGSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMDSPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRKNTALVKNNREGIQRVLTTDYALLMESTSIEYISQRNCNLTQIGGLIDSKGYGVGTPIGSPYRDKVTIAILQLQEEGKLHMMKEKWWRGNGCPEEDSKEASALGVENIGGIFIVLAAGLVLSVFVAIGEFIYKSRKNLDVEEVSLNMRTSPQGGMPGRQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDZ6</accession>
    <name>A0AAR2JDZ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Gamma-aminobutyric acid receptor subunit gamma-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">GABA(A) receptor subunit gamma-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">GABRG3</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Gamma subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride across the cell membrane down their electrochemical gradient.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Heteropentamer, formed by a combination of alpha (GABRA1-6), beta (GABRB1-3), gamma (GABRG1-3), delta (GABRD), epsilon (GABRE), rho (GABRR1-3), pi (GABRP) and theta (GABRQ) chains, each subunit exhibiting distinct physiological and pharmacological properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG3 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JDZ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000088181.1">
        <property type="protein sequence ID" value="ENSPNAP00000048552.1"/>
        <property type="gene ID" value="ENSPNAG00000012942.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441187"/>
    <dbReference type="GeneTree" id="ENSGT00940000155607"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19000">
        <property type="entry name" value="LGIC_ECD_GABAAR_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19054">
        <property type="entry name" value="LGIC_TM_GABAAR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000006">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit gamma-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000003">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit gamma-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005437">
        <property type="entry name" value="GABRG-1/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01620">
        <property type="entry name" value="GABAARGAMMA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 24" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 24" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 24" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="26">
        <location>
            <begin position="114"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="27">
        <location>
            <begin position="326"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00064898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR605437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="513" mass="59249" checksum="57ACB4C52FB21CF9" modified="2024-11-27" version="1">MKRASLHFSTQTWTAFDAGLFWSMGSSREEAFTRVSVATLPQQSTGSPRCPRSMSHSERKHPPHLNMPTKLLVYFLLLSVFRACSQTIASEEEEYDDVTVNQMLAPRTHETDATQILNNLLKEYDKKLRPDIGVKPTVIDVDIFVNSIGPVSSINMEYQIDIIFAQTWTDSRLRYNSTMKILTLNSNMVGLIWLPDTIFRNSKNADSHWITTPNQLLRIWNDGKILYTLRLTINAECQLQLHNFPMDEHSCPLIFSSYGYPRDEMIYKWRKNSVEAADQKSWRLYQFDFMGLRNTTDIIKTTAGDYVVMTVYFDLSRRMGYFTIQTYIPCILTVVLSWVSFWIKKDATPARTALGITTVLTMTTLSTVARTSLPRVSYVTAMDLFVTVCFLFVFAALMEYATLNYYSYSARRPTCNKTKRSNYSVLDVGPPPTVIALNNSMYWQEFDDACIYECLDGKDCQSFFCCYEECKGGAWRRGRVHIDILELDAYSRVFFPTSFLLFNVVYWVGYLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JEZ0</accession>
    <name>A0AAR2JEZ0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JEZ0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076295.1">
        <property type="protein sequence ID" value="ENSPNAP00000048789.1"/>
        <property type="gene ID" value="ENSPNAG00000037123.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000279289"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="445" mass="51813" checksum="CF8E5951270CFC02" modified="2024-11-27" version="1">EELLNLSLNSPTNHHTLAVKPDFCCPKHRLQQTDRVKYSLQQTGRVKHPLQQADRVKHPLQQADRVKHSLQQTGRVKHSLQQTGRVKHSLQQTDRVKHLLQQTGRVKHSLLQTGRVKHPLQQADRHSLQQTGRVKHSLQQTDRIKHSLQQTDRVKHPLQQTGRVKHSLQQADRVRHSLQQTGRVKHSLQQTGRVKHSLQQTDRIKHSLQQTDRVKHLLQQTGRVKHSLQQTGRVKHSLQQHPLQQTDRVKHSLQQTGRVKHSLQQTGRVKHSLLQTGRVKHLLQQTGRVKHSLQQTGRVKHSLQQADRVKHPLQQTDRHLLQQTGRVKHSLQQTGRVKHSLQQTDRIKHSLQQTGRVKHLLQQTGRVKHSLQQTGRVKHSLQQADRVKHSLQQTVRVRHWLQQTGRVKHSLQQTDRVKYSVQQTGRVKHSLQQADRVKHSLQQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JMI9</accession>
    <name>A0AAR2JMI9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sclerostin domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sclerostin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552217.1">
        <property type="nucleotide sequence ID" value="XM_017696728.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JMI9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080434.1">
        <property type="protein sequence ID" value="ENSPNAP00000051066.1"/>
        <property type="gene ID" value="ENSPNAG00000037322.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426914"/>
    <dbReference type="CTD" id="550261"/>
    <dbReference type="GeneTree" id="ENSGT00390000014900"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008835">
        <property type="entry name" value="Sclerostin/SOSTDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14903:SF5">
        <property type="entry name" value="SCLEROSTIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14903">
        <property type="entry name" value="SCLEROSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05463">
        <property type="entry name" value="Sclerostin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043501743" description="Sclerostin domain-containing protein 1" evidence="11">
        <location>
            <begin position="24"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="12">
        <location>
            <begin position="86"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="185"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="223" mass="24726" checksum="B7C61BA2D0692A8F" modified="2024-11-27" version="1">MRVRSEPCSFVLLLCVLMRSAAPLSNHNTELRHTTQTTHTSRSEHSEHSTEPDTVGAAALNRARSAGRGLENAGARGSGDRGPVGCRELRSTKYISDGQCTSISPIKELVCAGECLPAQMLPNWIGGHGRKFWNRRGSSNQDWRCVNDKSRTQRIQLQCQDGSTRTYKITVVTGCKCKRYLRQHNESGNKSESISQPQPIHKPKTKQKNGNSKAIGNWPELES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JPY3</accession>
    <name>A0AAR2JPY3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dedicator of cytokinesis 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DOCK9</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000046814.1">
        <property type="protein sequence ID" value="ENSPNAP00000054078.1"/>
        <property type="gene ID" value="ENSPNAG00000022518.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155972"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08697">
        <property type="entry name" value="C2_Dock-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11698">
        <property type="entry name" value="DHR2_DOCK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13267">
        <property type="entry name" value="PH_DOCK-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000016">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 9 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037809">
        <property type="entry name" value="C2_Dock-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF77">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="185"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="6">
        <location>
            <begin position="647"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="7">
        <location>
            <begin position="1610"/>
            <end position="2063"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="2033"/>
            <end position="2060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2102" mass="239158" checksum="EAEC2B4E4773520C" modified="2024-11-27" version="1">MFHFQDLGLCSQSASAGGGACRKTGGEKHRRAGQRCGGTMSLPPQTETRKFTRGLSKPGTAAELRQSVSEVVRTSVLVVKPRVIEPLDYESVLVQRKTQILSDVLRDMLQFPLEDFQVCILDACIKTYKSDWHVANYKYEDYSGDFRQLPNKVSRPDKLAVHVFEVDEDVDKDEDTSSLGSQKGGISKQGWLHKGNMNSAISVTMRSFKRRYFHLTQLGDGSYNLNFYKDEKISKEPKGTIFLDSCMGVIQNYKVRRFAFELKMQDKSSYLLAADSETEMEDWINTLNKILHSSFELAMQEKRNGDLHDGLFLCASNLSCLTKCASLQSARDIESKIRNETRLKLFTLDPDTQKLVFSGIEPEVKPFEEKFGKRILVKCNDLSFNLQSCVADNEEGPTTNVEPFFVTLSLFDTQNSRKISADFHVDLNHQSVRAMVPGSGAQIINGASDAAQRTTNGLPESALQYPRQGVFSVMCPHPDIFLVARIEKILQGGITHCAEPYMKNSDSAKTAQKVLRNAKLACSRLGQYRMPFAWAARPLFKDASGTLDKSVRFSAIYRQDSNKLSNEDMFKLLADFRKQEKMAKLPIILGNLDVTIDSVAPDLPNCITSSYLPVRQFESSDWNGLFFEVEEFVPCLAKCSQPFTTYNNHLYVYPRHLKYDSQKTFAKARNIAVCVEFRDSDEEDALPLKCIYGRPGGHHFTKNAFAAVLHHQQNPEFYDEFKIELPTQLNEKHHLLFTFYHVSCDSNSKASTKKREQVETQVGYAWLPLLKDGRVIMNEQHIPVAANLPAGYLSYHDSNGKHAGPEVKFVDGGKPLFKVSTHLVSTVYTQDQHLHNFFLHCESSVSTPQPKGGELVKYLKSLHAMEAHVMINFLPTILNQLFRVLTSAAHEDVAVNVTRSYVFYHSQCHEEGLEHHLRSYVKYVFKTDANTATGKTVHEELAKAMTTILKPSTDFLTSNKLLKYSWYFFEALVKSMAQYLIESGKVRLSRNQRFSASFHHTVETLVNMLMPHIVQKYKDNLDAACNANHSLAFFIKRCFTFMDRGFVFKQINNYINCFMPGDAKTLYEFKFEFLKVVCNHEHYIPLNLPMPFGKGRIQRFQDLQLDYSLTDDFCKNHFLVGLLLREVSGALQEFRDIRQIAVQVLKNLMIKHTFDDRYTSKSQQARLATLYLPLFGILQENVHRLNIKDTSPFTNSLSNSHSRDEPLLTSSLMTPPKAGGHIESSLHKDVFGVISGTASPHTTSTPNVSSVRHADSRGSLISTDSGNSLSERHIDKHHPLDKCHVAAALGGTLLRCDKLEQSEVKSLLMCFLHVLKSMSEDALFTYWNKASSGDLMDFFTLLELCLHQFRYMGKRYIASRAGMMHARLQQLSSLDNSYTFNHSYSHSDADVLNQSLLEANIATEVCLTVLDTLSIFIMGFKTQLSLDHGHNPLMKKVFAVHLSFLQINQSETALKQVFTFLRTFIYKFPCTFFEGRADMCASFCYEILKCCNSKLGSIRSDAAHLLYFLMKSNFDYTGRKSFVRTHLQVVIAVSQLIADVIGIGGKRFQQSLSIINNCANSDKTIKNTAFPSDVKDLTKRIRTVLMATAQMKEHERDPEMLVDLQYSLAKSYASTPELRKTWLDSMARIHVKNGDLSEAAMCYVHVAALVAEYLRRKGECQSTKTCLWPNLPHYFCTGAKRLIWMFKQGCSAFRVVTPNIEEEASMMEDVGMQDVHFNEDVLMELLEECADGLWKAERYELISDIYKLIIPIYEKRRDFEKLSHLYETLHRAYSKVTEVMHTGKRLLGTYFRVAFFGQGFFEDEDGKEYIYKEPKFTPLSEISQRLLKLYSEKFGAENVKMIQDSGRINPKDLDPKYAYIQVTHVTPFLEEKEIVERKTEFERSHNICRFVFETPFTVSGKKQGGIEEQCKRRTILTTTHFFPYVKKRIAVMYQHHTDLNPIEVAIDEMSKKVAELRQLCASSEVDMIKLQLKLQGSISVQVNAGPLAYARAFLDDTTSKRYPDNKVKQLKEVFRQFVEACGNGLGINERLIKEDQQEYHDEMKANYRDLTRELSAIMHEPICPVEDAMKSTLPDSLHIFNAISGTPTAASIQGLPSSSSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JWJ8</accession>
    <name>A0AAR2JWJ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Desmoplakin a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the organization of desmosome cell-cell junctions. Of particular importance in cell adhesion in the skin and during cardiac development. May also play a role in the regulation of Wnt, TGF-beta and Hippo signaling pathways.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the plakin or cytolinker family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000043668.1">
        <property type="protein sequence ID" value="ENSPNAP00000054236.1"/>
        <property type="gene ID" value="ENSPNAG00000019131.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154843"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002934">
        <property type="term" value="P:desmosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061436">
        <property type="term" value="P:establishment of skin barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.780:FF:000001">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000001">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000002">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000010">
        <property type="entry name" value="plectin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041573">
        <property type="entry name" value="Desmoplakin_Spectrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF26">
        <property type="entry name" value="DESMOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00681">
        <property type="entry name" value="Plectin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18373">
        <property type="entry name" value="Spectrin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21019">
        <property type="entry name" value="Spectrin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21097">
        <property type="entry name" value="SR_plectin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Desmoplakin SH3" evidence="13">
        <location>
            <begin position="320"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Desmoplakin spectrin-like" evidence="14">
        <location>
            <begin position="420"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1122"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2513"/>
            <end position="2550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="181"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="254"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="849"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1246"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1467"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1124"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="2513"/>
            <end position="2522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="2523"/>
            <end position="2546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2569" mass="294965" checksum="8943F3936A284C3B" modified="2024-11-27" version="1">SQHKQSQHKYSQHKHNTVNKHSQHKQSQHKYSQHKHNTVNKHSQHKHILMCCDCFGDVLCSVVFCAQRLIETSPFGDDAASIEQQIMNHNKFHSSIQRSTEFQRARDELMQKGDKSSLHNVEQEWDSLQKLSYARMGQLRDLQSIIEEISTAIMWVNDREEEELVFDWGDKNIDDYIPKKQESYSRLMRDLEEKEKDLNKLKTKVDQLLKNQHPASDKIEAYMDTLQTQWSWLLQITKCIQVHLKENAAYSQFFKEANETYTKLQKDHENIRKKFTCDKETPLENLTDLLKNLEKEKERLMENKRQVQTLVNKSKSIVRLKPRYPEEKSSSSVIVKALCDFKQDQKGILKDNEAILKDNSQRSKWHVTGPGGLDMLVPSVCLIVPPPNPLSINLATKNEQYYEAIMSIWNQLFINIKSLISWQYCIKDIKYIKSLTLSMLSQMKPEEYRNIIKNLELHYQEFLRLSHGSDMFGVEDKAKIEAEYSGAQSHYEVLLTKLPNLNVTPGAGHSSSSGQTSSTLSLNLLTQIHTLRRRLEAAETGLSQHLHIPLKDNSVQECSQRLQLLQGVHRDLDSIRDEYLRVREMVVRQLEGAGDPEQAKFLRSELDLINQKLGSLQGFSAAYLQRLTALRALLQSLTRAEDIIKVHEARLTEKETSSVDPAQLQEYCSTLKVMKGDLEQKEDVLTSLESELAKAIHWNSQIDQSFHQCDVDLAKYAELVGQMSDRWRRILTQINSRMWDLEKQEKQLTHYQQTSTQLSQWIDNTRQRLDGLQTAKFTSVQSLMDQLNQQKALHSEIKVKKEKVEDVQKDASTCAASIKDYELQLASYSAGLETLLNIPIKRTMLQSPATELSQEAADLQSRYIELLTRSSDYYKFLGEMLKNMEELKMRNTRIELLEEELRRLKEDLQERSQKNKSLEDTLARYQLELSHSKEQLITMEEVKSTEAIRHHATKENLDTTQNQLRDLNEQVARLTLLIDEEKRKRRLAEERYTSQQTEYETAIRKRQKELDELNWSKIDFEKAIKDKEREIERLKMQLEDEASRRRAAESETSKVRNQYNQEISSLKQTYESEIHITKTSVLKATQQKEDDTAALKLQLERLLDEKRDVEEELRRLRVSISQTEEARQRAENDAHQQKSSVTEETRRRKELDVQVQTITRQRTELEARYKEELAQANAGAQEKIRQIALLKQNLEDETRQRKALEVENQNLRQSKAELHAKHTSSLDVINKLKLTEQETTLVRVELEKLTSEKGKMEQNASRLQSRIRELQAMVDSLEAELEKEKKSTQDELTRRKRIEAELERVNQTCKEYTTTINTLRIHKEEESATGRRYEQDLRRVQEELDRSLREQKATSENLAKLTAELKALQQQLLQEQARVREVNLRNETLYKTIEEKSRLLNDSTSEVEKLQSLTQNLTKERLKLEEELRGVRQERDDLKSSWNSTESEHTSQLSTIQIQLQDASRRGLEYQAQINVLTKERENLKAEIAKTQKQYAETSLMMHESQTRYNEILQERDGLLAKLKLLEMDKGKQQRYEEELSRIKVSLESELRQKQRLQDEMDKIRKDFNYWKSQYEIKDGQVRQCEADKDKAERDRASLRSEIARLTAELRSVEERYRTRLQSSESEVSELTRKREALERELMKLRQRPDAYSRQTQTDEKVTTIDPSKLLFDGVRRKVTAHQLCDCGIIDKATLDKLLNGQKTVEEVAVDIQLNLKGTGIIAGVAQGSQNRLPLTEARSKKLLSPESAIMLLEAQAATGHIIDPKFNEKMSVDAACSRGVVDTQDRDALITAEAACIGFKDPFTGKLLSVGQACKKGRLDKETALRLLQAQEAVGGIIDPVLSVFLPKDTALDRSLIDEDLYRALNKKPTCYIDPANGEKITYIELRKKCITEPAAGLLLLRANDKSTTVQGIRGQVPLSELVKSKLITESDLEKVNQGLLSTKDIENRLKSYLGGSSCIAGIYDEAHDRVLPFYQAMKEGLLRPGTTLELLEAQAASGFMIDPVNNVCMTVEEAWKRSLVGKEFKDKLMSAERAVTGYKDPHTGKLISLFQAIEKELIEKGHGIRLLEAQIASGGIIDPIESHRIDVEMAYKRGYFDHEMNDILTYEGDDTKGFFDPNTHENLTYLQLKKRCITDPKTGLILLPLHDKKKAKQQTTQKNTLRKRRVVIVDPDTGKEMSVREAYHRELIDYDTFLDLSEQECEWEEITITDSDGKTRLVIVDRKTGNQYDIQDSLDRGVIPKSALDQYRSGSLTLTQFANLITTKSNSSELAICTSSVENIATYSSPTEASPSSPTVRKRLASVSITLSPPSDILDDQSPVAAIFDTETLEKITIAEAQRRGLVDSISAQRLLEAQACTGGIINPATGKRLSLQDAVHQGVIDENMAAKLKAAQKAYLGFEDVKTKRKMSAAEAMKEKWLPYEAGQRFLEFQYLTGGLMEPTTGKRVNIEEAIRRGWVDGKEAQKLQDTRSYVKNLTCPKTKLRISYKEAMDNCMVEEENGMKMLQASSMSTKGISSPYNVSSGPGSRTGSRGSSRTGSRSGSRRGSVDYSSTYSYRFSSKLLVFVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K2H5</accession>
    <name>A0AAR2K2H5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">EPGN</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K2H5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079872.1">
        <property type="protein sequence ID" value="ENSPNAP00000056697.1"/>
        <property type="gene ID" value="ENSPNAG00000033301.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000113053"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005154">
        <property type="term" value="F:epidermal growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045840">
        <property type="term" value="P:positive regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10740:SF10">
        <property type="entry name" value="EPIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10740">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00009">
        <property type="entry name" value="EGFTGF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="142" mass="15131" checksum="3390B6F0FFED612C" modified="2024-11-27" version="1">QNLTSPIASPPAVTAVATVLLLYSKTAEPAAEPNTTHSVELLTHGEEPQALAMQRPCGSEHNSYCIHGTCSYAPDLNEPSCVCAPTFSGPRCEHVLFVILSPTQPEEIIGITCGVLLLLGCIAVISYICPTYLNNHSSGQYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K6X7</accession>
    <name>A0AAR2K6X7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Telomere length regulation protein TEL2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TELO2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TEL2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037392234.1">
        <property type="nucleotide sequence ID" value="XM_037536337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K6X7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070355.1">
        <property type="protein sequence ID" value="ENSPNAP00000058207.1"/>
        <property type="gene ID" value="ENSPNAG00000028302.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415756"/>
    <dbReference type="GeneTree" id="ENSGT00390000006698"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.720:FF:000001">
        <property type="entry name" value="Telomere length regulation protein TEL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.720:FF:000003">
        <property type="entry name" value="Telomere length regulation protein TEL2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.720">
        <property type="entry name" value="Telomere length regulation protein 2, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038528">
        <property type="entry name" value="TEL2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051970">
        <property type="entry name" value="TEL2_Regulation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057348">
        <property type="entry name" value="TELO2_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019337">
        <property type="entry name" value="Telomere_length_regulation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15830:SF10">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25320">
        <property type="entry name" value="TELO2_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10193">
        <property type="entry name" value="Telomere_reg-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TELO2 ARM repeat" evidence="11">
        <location>
            <begin position="288"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Telomere length regulation protein conserved" evidence="10">
        <location>
            <begin position="524"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="429"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="841" mass="94008" checksum="29AE81FEBB0C5F3C" modified="2024-11-27" version="1">MDRCSGAEVEVRQAVAPCLRIVSSSRDSAEVTEALRTIIHYLEGGNASASEKAEFNRANYTRVLQVLVSSLSSDWFHSLPAQERRELWDGFFLRGPPDQALLVLLDTISSTSDGSGLDWTVSVLESFLSAGRVGMLLWSRCLETNGRPDSVQLRETILTRLSSLPDLTANRLRTNTPARLLPQQYYTDLARDVLNTLENTCRALRAGQDCSLGFVAQLLGKVCMQGHGELVCRTLAPRLSSLTRSDALWRRVSCKLLENVPDRWVESVITALIHHLHGASALSRVMGNLVLKNKKVQFVLTHKVLLLQYQHPTQLLRTVLGYLALDHDRRPLLKQVLRSVCQVWSNSSAVRHTSLDQQLYVSKALLLCVNLLNQTEIQELREELLQCMLGGVQCRLDSSTEHVRRMGMVVGECLSQKLDTPTSQLQFQYEANEESRELLSLMEPQSEEEDEDEQQQQPDDAPRPQPESSGQSAEGQSMMSQPPAASQTSAKPAGHSSDSELDSDDELTPYDMSADQVKQKATPPRYVRDCLEALMSSDDPERVELSLQTAEELLRKNVSTAREVSVQFSKVLLHLEDRYSTAGFHTLRQSAMVALAVIHTQPVVEYLTTEFYSLNYSLRQRLDILEVLALAAQELSEPITEKKAVSQPVARAVTLEQCGEVEHWRQVVEKRIQSKTRRISKGASQPPKAAPNRYAPVAAYFFFPLLRNYDRPQVTFDLLGSDHLVLGQLVHTLGLLMHLAVNAPVATQMGKALLDFVWAIRFHADQMVRRGVLFAVCAVFLSMPGENLLLELSDDLVETRAWLADVAESDPDADCRSLAVQSLVLLEKNLKTHLQSPSMET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K8I3</accession>
    <name>A0AAR2K8I3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Kinesin family member 13A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KIF13A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037390061.1">
        <property type="nucleotide sequence ID" value="XM_037534164.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000047687.1">
        <property type="protein sequence ID" value="ENSPNAP00000058799.1"/>
        <property type="gene ID" value="ENSPNAG00000009018.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426383"/>
    <dbReference type="GeneTree" id="ENSGT00940000157508"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22729">
        <property type="entry name" value="FHA_KIF13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000015">
        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000002">
        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000010">
        <property type="entry name" value="Kinesin family member 13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="5"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="14">
        <location>
            <begin position="1815"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1507"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1624"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1740"/>
            <end position="1768"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="364"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="610"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1625"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1746"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="102"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1879" mass="209611" checksum="9FDAFD16C06665DD" modified="2024-11-27" version="1">MSDTKVKVAVRVRPMNRREIELSTKCVVEMEENQTILHPPPSNTKGDSRKQPKVFAFDHCFWSMDEANVPKYAGQEVVFKCLGEGILQNAFQGYNACIFAYGQTGSGKSYSMMGSVDQPGLIPRLCCSLFERVGREENETHSFKVEVSYMEIYNEKVRDLLDPKGSRQSLKVREHKVLGPYVDGLSQLAVTNFEDIESLMSEGNKSRTVAATNMNEESSRSHAVFSIIITQTLYDLQSGNSGEKVSKVSLVDLAGSERVSKTGAAGERLKEGSNINKSLTTLGCVISALADQSAGKGKNKFVPYRDSVLTWLLKDNLGGNSKTAMIATVSPAADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELREEVEKLREQLSQAESLKAPELQEKLQESEKLIQEMTVTWEEKLRKTEEIAQERQRQLESMGISLESSGIKVGEDKCFLVNLNADPALNELLVYYLKVHTRVGADTSQDIQLFGIGIQPEHCVIDVAPDGDVTLSPVENARTCVNGTIVYSLVHLWHGDRILWGNNHFFRVNLPKRKRRDRLKDLDRESPRESFVEVDVETASEASSEQDYSYEFAQMEVMMKTLGSNDPMQNVVQVLEKQYLEDKRTALEEQKLRYEHELDLLRQQLSPERVSQHQRSNSLAFPTHTTHNKLRLWTEERDELFRQSLSRLREQVVRANTLVREANFLSEEMGKQTDYQVTLQIPAANLSANRKRGAIVSEPAIQVRRKDKGTQVWTIEKLENKLVDMRDHYREWKEGAQDGATKLQNKQGDPFYEAQENHNLIGVANVFLECLFHDVKLQYAVPIISQQGEVAGRLHVELMRVSGAVPERLVGGDDSSENSSESSSYEVMDTNGEIVHMAKKLTCRVRIREATGLPLNLSNFVFCQYTFWEQAEPAVAPPMVSPDKPSTRTTDAQFTVLFNHCKDFVVQVTEEFLEFISDGALAIEVWGHRCAGNGHSRWELDTLQAKTRTLRDRWSEVSRRIELWVSIQELNEQGVYSPVELHPSRDTSTGGVFQLRQGHSRRLQVCVKPAQQSGTLPLLVEAILSVSIGCVSARSTKLQRPLDSYQREEDDDMDSYQEEDLNCLRERWSEALIKRREYLDEQIKKIIHKSEKSEEDVEREARLVEQWVGLTEERNAVLVPAPGSSIPGAPAQWAPCPGMEAHIPVLFLDLNADNLTVSEQLTGPHAAGVNSILPKEHGSQFFYLPIIRHSDDEVCATCSWDSSIHDSIYLNRVTAPTERIYLIIKATVQLSHPASMELVLRKRIAVNIYNKQSFTQSLKRRMSLKNVLHSCGVTYEIVSNIPKASEEPEERETLALMAARGEAEETQDGETYIEKYTRGVLEVANILCLDRLRQAVTVKEALSVKSRHIRRSLSTPNVQHMSCCKSETLGCEDEDPKGHCEVQQDVSDCPFQEILSAAPAKDTPVKNKENHGCVPESPMFFSSSPFKALSPQPPKFLKSLMPVREESKAKRSLEARPLLGQESMRWLVEGSVLPRPRARSEGHSSTTNHSPLRVTHTADSEEEEPELDPAAGLSSLPNFQPYVPEDFANFEVYNASLETQDATLCGGMASYPGNKVAEKEVSRSPTSSSCTSGYFSHSASNATLSDALFTGSESSDQLSSGSRDPADSLLNKNSFGEAGHCHPGPVHSSMNCTGQSCSPKANQCILSVSQEFTDFKGAGDGFSFESECQTEMPESLRQAKCDQTPPRNTEGISQSLCTNVQATPCHLHNGKDSPSSSSSSTTPTQPEGSSLEDHMFSKTTVETGSSAPEEDEGAPVAQLPDWMAPGEQVWVGTRSGTVHYVGGVEFAQGIWVGVELDRAVGKHNGTVQGRVYFRCASGHGVFVRPALLSRVPPAMDMDPPPQPVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K9U2</accession>
    <name>A0AAR2K9U2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MXD4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K9U2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067908.1">
        <property type="protein sequence ID" value="ENSPNAP00000061040.1"/>
        <property type="gene ID" value="ENSPNAG00000001830.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160120"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000014">
        <property type="entry name" value="Max dimerization protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969:SF4">
        <property type="entry name" value="MAX DIMERIZATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11969">
        <property type="entry name" value="MAX DIMERIZATION, MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="53"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="246" mass="28423" checksum="EFA9D3694CB23221" modified="2024-11-27" version="1">MELNSLLILLEAAEYLERRDREAEHGYASVLPFNSDFSRKKTKATPISRKAQSNRSSHNELEKHRRAKLRLYLEQLKQLVPLGPDSTRHTTLSLLKRARMHIKKLEEQDRKALNVKEQLQREHRYLKRRLEQLGVERIRTDSMGSTISTDSEQGKHTHTHTHTHTHTHTHSTGSTISTDSEQGKHTHTHTHSTGSTISTDSEQGKHTHTHTHTHTHTHTAQAPPSLLIQNKVNTHTHTQHRLHHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KDW8</accession>
    <name>A0AAR2KDW8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphofurin acidic cluster sorting protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PACS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037394699.1">
        <property type="nucleotide sequence ID" value="XM_037538802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KDW8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056544.1">
        <property type="protein sequence ID" value="ENSPNAP00000060674.1"/>
        <property type="gene ID" value="ENSPNAG00000032457.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411050"/>
    <dbReference type="CTD" id="557305"/>
    <dbReference type="GeneTree" id="ENSGT00950000183209"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019381">
        <property type="entry name" value="PACS1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057541">
        <property type="entry name" value="PACS1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280:SF14">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25332">
        <property type="entry name" value="C2_PACS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10254">
        <property type="entry name" value="Pacs-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 N-terminal C2" evidence="5">
        <location>
            <begin position="53"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 C-terminal" evidence="4">
        <location>
            <begin position="500"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="359"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="436"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="734"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="913" mass="100588" checksum="CC16F93CFC28EC75" modified="2024-11-27" version="1">MMAAAADRGALRAAVLLSPPCGGAVSACAGASGAGVLSPGSVVVGPVPAPLTVPMNLFATWEIDRSSPSCVPRLCSLTLKRLIILRELDKELNSVVIAVKIQGSKRVLRSNEYVLPPSGLMETDLELTFSLQYPHFLKRDANRLQIMLQRRKRYKHRTILGYKTLALGVINMAEVMQHPTDGGQILGLHSNMKDVAARVAEISVFSLSSQPIDHEDGGQSRNKNKNNDRSPDMDNYSEEDDDSYSSEQEASDDAVHGQDLDDEDEDVQKPKKSRRIVIRTTSMTRQPNFKQKFVALLKRFKVTDEVLDSDPVDQTQEVEDLDLLYDSLEEHNHSDSGPDVEDNESVLSTPKPTLRPFFEGMSHSSSQTEIGSVHSQRSQQQDTGCGLLPAAPEERQQEETSTNTNSGEAEGEDGGLVMGDETCTDRLTGSTARLCKTEAQNQVSPSKVDSRLAMRGRSLKDRQNSRNQTDRTISVESENSPDSRQPAQILDLLQVPRKSVYDQLNQILISDERLPETIILINTVDWQGQYVAEVLRKQAQPIVSTCSSADVQAAFNTIVTRIQRFCNCNAQTPPTVKVAVAGDQSYLSTILRFFVEQLANKTPDWLSYIRFLLIPIGSHPVAKCVSAFDSRFGGMFMDTAWRELFCRTEPPAADSVDVAGRILQYLAGANVSHQFPISEAMLTYRHKSPDEDSCQKFVPFIGVVKVGIVEHSLSTSVDSDDAMGGGIGILTTPPSQPATTHGREMCVTPPPSPSTCTALTGVGSPGAGGELMGLQVDYWTMQGGEKKRDGEKREAGLKNTLKSNFRFLQVSRLPCRGELTPPPSMAMTVVTKEKNKKVIFLSKKVKEKELDSKSQVIDGISRLICTAKHQHTMLRVSIDGVEWNDVKFFQLAAQWPTHVKHFPVGIFSYSKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHN1</accession>
    <name>A0AAR2KHN1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cullin-5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 11">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHN1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048690.1">
        <property type="protein sequence ID" value="ENSPNAP00000061942.1"/>
        <property type="gene ID" value="ENSPNAG00000024709.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156648"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000142">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000009">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000014">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000017">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000004">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="12">
        <location>
            <begin position="403"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="780" mass="91128" checksum="A04530760DA84FFB" modified="2024-11-27" version="1">PIKRQNHQNKGSLQFEDKWDQMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILDFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPDDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLREEEKRALRYLETRRECNSVQALMECCVNALVTSFKETILAECPGMIKRNETDKLHLMFSLMDKVPSGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPMKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIELKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQVFKEMHKHNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEDFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLSYEPQVSSPKDFTDSTLFFVNQEFSLIKNSKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKITNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIKRDESDINTFIYMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KP71</accession>
    <name>A0AAR2KP71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Stabilin 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">STAB2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000042193.1">
        <property type="protein sequence ID" value="ENSPNAP00000066075.1"/>
        <property type="gene ID" value="ENSPNAG00000029851.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156566"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000032">
        <property type="entry name" value="Fasciclin domain-containing protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000001">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000040">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000005">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000017">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000018">
        <property type="entry name" value="Stabilin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056806">
        <property type="entry name" value="EGF_STAB1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24038">
        <property type="entry name" value="STABILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24038:SF0">
        <property type="entry name" value="STABILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24887">
        <property type="entry name" value="EGF_STAB1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="5 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043479217" description="Stabilin 2" evidence="14">
        <location>
            <begin position="17"/>
            <end position="2496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="2404"/>
            <end position="2428"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="93"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="140"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="179"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="220"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="305"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="16">
        <location>
            <begin position="347"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="16">
        <location>
            <begin position="497"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="717"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="807"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="845"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="888"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="931"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="16">
        <location>
            <begin position="952"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="16">
        <location>
            <begin position="1095"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1302"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1390"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1430"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1507"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="16">
        <location>
            <begin position="1548"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="16">
        <location>
            <begin position="1680"/>
            <end position="1821"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1897"/>
            <end position="1937"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1944"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="2021"/>
            <end position="2061"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="2062"/>
            <end position="2104"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="17">
        <location>
            <begin position="2137"/>
            <end position="2230"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="16">
        <location>
            <begin position="2250"/>
            <end position="2388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2445"/>
            <end position="2496"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1332"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1394"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1398"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1927"/>
            <end position="1936"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1971"/>
            <end position="1980"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="2159"/>
            <end position="2228"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="2183"/>
            <end position="2204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2496" mass="270776" checksum="5B673836256E5665" modified="2024-11-27" version="1">MWLLCVCRHDLFSVCAHVYVCVCVTTKTDCHSCSISSVVNCPEGYQKKTPGRGKQDCRYNLKTISNLSIPGCSHECYKEEVEQRCCPGYWGIDCIECPGSAATPCNRKGVCSDGMGGNGTCTCKAGFVGTACEECDENLYGPMCSTVCTCKHGLCNSGLKGNGQCTCFSGYTGPDCDQELPGCAALRCGPDALCFEDVSSGKLACKCKSGYAGDGVQCTSINPCVRSVCHQHAVCVHTGPNQHTCTCTEGYQGDGLVCLPIDPCQTNFGSCQSHSTRCVYDGPGKAHCECLPGFEKLVMGVGCSLRDACKPDSCHKNAYCTNVAPGTVECTCRQGFLGNGKICFGNIMQRLQDLNSEPGSGWTGQLSNGIRLFEAAMSWPLTSLGPFTVFVPSNKALKSTSVKTLVADEAKARYLVKMHMVAGEVTSDFLKKGILYYTLTGKLAESITESQQVKIRIHGSRRKGTLVESDVIASNGIIHIIDKMMDTVPSTVISDNQENLMKILSDNSKFSQFKSLFEKTTMADTLDQAGPYTVFAPTNTAFSLLKDGYLDYLTSEEGKPKLLELLRNHIVASAQLRASYIVSNPRTESMANQVLEFNVTTAGQILINGIAVVEADVEAKNGRLYSLDGVLIPQSIEPILPHRCDTTDKYIYKAPCVSCRFVSLSTCPTGASLNTFSRGCIYTQKTLGMKVPVLGCSLFCNETRVTPKCCEGFFGQDCSPCPGGFSTPCSSHGKCFDGINGNGTCQCEPNFKGSRCQYCADPNKYGPSCDQTCGCVQGVCDNRPEAHGTCKPGSCEPGFAGQLCDKHAQPCGPNQPCHAHANCVYSEGACVCKHGFQGDGFLCAEVDACASPPNGGCSKNANCIKTGPGTHRCECVKGWREDGDECQPVNNCLEPSRGGCHPNASCIHVGPGQNYCVCKQNYRGNGRECEAVNECVEQIGGCHPRALCLSVSQRRFQCVCQPGYLGDGTSLLFWNNSQQADLTQLLSETHNLTLFVPSAQAISNLSKEDQDFWLSSSNLPSLVKSHIVSGLFGLSDLRSSPSPQITSLLKRTLPVSWTNETTTVAGAAVLSGDMLAKNGLIHLINTVLVSDRKLSEGLLEVLNQRPEFSLFMSSLTNYNLTETIEEASGFTVFAPTNTAITEYLQSTGQDSLDVNVTKYHIVLGETLKQGDLQDGLYKDTMLGFSYQLGIFRNDSNWCVNKAQLSATDIESTKGVVHGLSAVLKIPNNRCDTASSKIIRVWSGQCMDCFYPGERRCPPGTEEVKTRKCTYSRRKHGMFIGCQASCRKLTIKRKCCEGFYGTNCENCPGPEGQPCFSNGVCMDGINGTGVCQCNPGFNGTACETCQIGKYGIHCDQDCKCVNGWCNDGLEGDGTCQCEVGWRGVSCSVAITTDSCGGKCHTSANCLLKVENQSYYCSCAAGYQGNGTYCTAVNACEKDNGGCSYNAVCKRTLPGRRQCVCNPGFAGDGKVCVCKCYFIFSNDFCVFVFLKTSCVCKPDYSGDGKTCSPVNLCSKKNGGCHKHATCTMTGPGERNCSCPRDFIGDGITCKDNVMQELRNRGLTDFFYNLLRADISFLQGRGPYTMFAPNKLAFNKPEVNTKEGRAALMMYHIVSCQSLLPEDLTQPRNLTTLTGEVLTISYSKGTVYINNKVKVVYSDQESSNGIFHEIDTVLTPPSFQKLSPPTLKDIANTHGFKTFIKLLEDTEVMKKVMDPLHLPVTLFLPTDLTMAALPQQQKDFLYGLHNRAQLIEYLEYHILRDMKVQSAEVIHLNSLKTLQGSEISVSCVGEKQIGELYINDKSCRIVKRSLNFPGGTVFGIDCLLTPPSLGGRCDHKDVVDITVSSAFLSLSSDCPAVSKPKTVEKCDLPRRLLAVNTGCQSVCSMVIWKPKCCSGYYGRDCLACPGGPGSPCSNHGQCDEDHLGNGTCTCNTGFQGVACELCTEGHFGPDCKACNCTEHGMCDEGPEGTGSCFCNEGWTGPRCEHKLADGPLCVPACHKKAVCAENNTCVCKPFYEGDGITCKVTDLCQFWNGGCAKNAECSQKEEKVNCTCLKGYSGDGLVCSPVDPCVVEDNGGCHEHAICTMTGAGKRKCECKPNYVGDGLMCELKVLPVNRCMQDNGQCHSDAQCTDMHYQDKTVGVFHYRSPLGTYKLNYTQAEEACRHEGGTMATYIQLSYAQQAGYSMCSAGWLAEKRVGYPMSFSNAKCGFGHVGIVDYGTQKDLSKTWDTFCYRIKDMKCECKVGYIGDGYSCTGNLYQVLSTKPDFSNFLSQILNYSKASAAGQEFVKRLTNISIQSTLFVPDNNGLYENETLSYRDIEHHLLDGRALMLQGLINVTHVRTRLGHTLNVKGVASLQDPQTQASSGYINDRYIIEPDILTSNGIIHVLQGPLKAPPPPPPSLPQAHKAGMGIGILLLVILIVAAVFVGYHFYNQQSKPFQFHYFKEDEREDTSPSDSTPYICNPTYEATPAPSSTEPPMENKEDKHQVVSGGSYDLLQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KXQ8</accession>
    <name>A0AAR2KXQ8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RASD family member 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539104.2">
        <property type="nucleotide sequence ID" value="XM_017683615.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KXQ8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057867.1">
        <property type="protein sequence ID" value="ENSPNAP00000066926.1"/>
        <property type="gene ID" value="ENSPNAG00000037061.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411848"/>
    <dbReference type="CTD" id="100006464"/>
    <dbReference type="GeneTree" id="ENSGT00940000165021"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF2">
        <property type="entry name" value="GTP-BINDING PROTEIN RHES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="330" mass="36409" checksum="D2D6B4A1B1CA0509" modified="2024-11-27" version="1">MESPAPARSSRLLLAYKSAAQHLLSKAGMRALQLQRSASAAAAKDKQRSSPIAQLAALVLQRRRRRRRLALESTALSLAPAPKPRNSRRVVVLGAPRVGKTSIVRRFLREDFEERYEPTREDFHRKLYHIRGEAYQIDILDPAGEREFPAKRRLTILTGDIFLLVLSLDDRASFDEARSLRSEISQAKSALLRCPAEGARVPVVVCANKADLPAEERAVSRAELLRAFGGKDTACTLFETSAKEGDETLERAFEALAARAGLPVETGPSHHRRVSLRSYRRLGGGGRKAEAHASPCGALHPLARRPSMNADLRLVLATHGKKRTSKPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KYY5</accession>
    <name>A0AAR2KYY5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bardet-Biedl syndrome 5 protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell projection</location>
            <location evidence="1 10">Cilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the BBS5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KYY5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065656.1">
        <property type="protein sequence ID" value="ENSPNAP00000069558.1"/>
        <property type="gene ID" value="ENSPNAG00000012464.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002753"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000232">
        <property type="entry name" value="Bardet-Biedl syndrome 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006606">
        <property type="entry name" value="BBL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030804">
        <property type="entry name" value="BBS5/fem-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014003">
        <property type="entry name" value="BBS5_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21351:SF0">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21351">
        <property type="entry name" value="BARDET-BIEDL SYNDROME PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07289">
        <property type="entry name" value="BBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010072">
        <property type="entry name" value="DUF1448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00683">
        <property type="entry name" value="DM16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 10" id="KW-0969">Cilium</keyword>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="354"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="BBSome complex member BBS5 PH" evidence="12">
        <location>
            <begin position="29"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="BBSome complex member BBS5 PH" evidence="12">
        <location>
            <begin position="159"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR010072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="383" mass="43691" checksum="D90E3A12A9F61E8C" modified="2024-11-27" version="1">MAGVLDALWEDRDVRFDITPQQMKTRPGEVLIDCLDSIEDTKGNNGDRGRLLVTNLRIIWHSIALPRVNLSVGYNSVINITTRTANSKLRGQTEALYILTKSNNTRFEFIFTNVVPGSPRLFTSVIAVHRAYETSKMYRDLKLRGALIQNKQLRLLPREQVYDKINGVWNLSSDQGNLGTFFITNVRIVWHANMNESFNVSIPYLQIRSIRIRDSKFGLALVIESSQQTGGYVLGFKIDPMDKLQDAVKEINSLHKVYSANPIFGVEYEMEEKPQPLEELTVEQPPDDVEIEPDEQTDAFTVSTLHSGMNDTLSRKLSGSLSCAENSNVLKFVPPTLTSVAWFQTCLHRKAFFIYFWLRLATILVIIYFFVIYAFFLFIGLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4M8</accession>
    <name>A0AAR2L4M8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L4M8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062533.1">
        <property type="protein sequence ID" value="ENSPNAP00000071558.1"/>
        <property type="gene ID" value="ENSPNAG00000031115.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164961"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="34"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="348" mass="40476" checksum="6B480A4F93003902" modified="2024-11-27" version="1">MKNGKAVGPDDMPVEVWRCLEEKAVDFLTRLFNRILESERMPDEWRSSVLVPIFKNKGDVQSCSNYRGIKLMSHTMKVWERVVEARLRREVQISEQQFGFMPRKSITDAIFALRVLVEKNREGQKELHCVFVDLEKADDRVPKQELWYCMRKSGVAEKYDRVVQDMYEDSETVVRCAVGVTNGFKVKVGLHQGSALSPFLFAMVMDRLTDEVRQEAPWTMMFADDIVICGESREQVEENLERWRFALERRGMKISRDKTEYMCVNEREAGGKLKMQGVEVVKMDDFKYLGSTIQSNGQCKKEVRKRVQAGWSGWRRMSGMMCDRRIAARVKGKVYKTALRPAMMFGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L781</accession>
    <name>A0AAR2L781_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L781"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065733.1">
        <property type="protein sequence ID" value="ENSPNAP00000070502.1"/>
        <property type="gene ID" value="ENSPNAG00000032882.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000245198"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="1">
        <location>
            <begin position="28"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="92" mass="10411" checksum="EE1621E7C2F22B7C" modified="2024-11-27" version="1">MWAFLVTHTLSLCLQVRRRLEMFTTLGRRVAANSTFPVSRPDKFSGDPDKCHGFLLQCSVYFENSPVNSDSAKLSFIVSRLADRALEWATAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LE46</accession>
    <name>A0AAR2LE46_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Activating signal cointegrator 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Thyroid receptor-interacting protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TRIP4</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription. May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a role in thyroid hormone receptor and estrogen receptor transactivation. Also involved in androgen receptor transactivation. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. May play a role in the development of neuromuscular junction. May play a role in late myogenic differentiation. Also functions as part of the RQC trigger (RQT) complex that activates the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with the thyroid hormone receptor/TR (via the ligand-binding domain); this interaction requires the presence of thyroid hormone. Interacts with the androgen receptor/AR; in an androgen, testosterone and dihydrotestosterone-dependent manner. Interacts with ESR1 (estrogen ligand-bound); competes with UFSP2. Interacts with UFSP2; competes with ligand-bound ESR1. Interacts with DDRGK1 and UFL1; the interaction with DDRGK1 is direct. Interacts with NCOA1. Interacts with EP300. Part of the ASC-1 complex, that contains TRIP4, ASCC1, ASCC2 and ASCC3. Identified in the RQT (ribosome quality control trigger) complex, that contains ASCC2, ASCC3 and TRIP4. Interacts with NEK6. Interacts with CSRP1. Interacts with ZCCHC4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LE46"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061185.1">
        <property type="protein sequence ID" value="ENSPNAP00000074868.1"/>
        <property type="gene ID" value="ENSPNAG00000020884.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005300"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06554">
        <property type="entry name" value="ASCH_ASC-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.30:FF:000004">
        <property type="entry name" value="Activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.30">
        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056994">
        <property type="entry name" value="TRI4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056993">
        <property type="entry name" value="TRIP4_3rd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23135">
        <property type="entry name" value="TRI4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23134">
        <property type="entry name" value="TRIP4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ASCH" evidence="21">
        <location>
            <begin position="428"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="385"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="573" mass="65265" checksum="9487EBDBC9587292" modified="2024-11-27" version="1">MMSDSLLRWCVDQLNNKFGLEASDDIVQYILSIDNADEIAEYVGDLLQGTGGKKKQFIDELLERWQWCRKQADSGPELIPMRETVTESPDVTKDTQKKAKRKGRNKQEVFSFSQAEPEPEAVKTPIDLLKAQENTSSSSTKKKNKFVNLYAKEGQDRLSVLLPGHHSCDCLAQNHKLINNCMTCGRIVCEQEGSGPCFFCGSLVCTKEEQEILQRDSNKSQKLRKKLMGDRVASEGLDILPHQEARIKAGLDKAIQHKDKLLEFDKNSVRRTQVLDDESDYFATDSNQWLSPAEREVLRKKEEALRELRHASRKDRKITLDFAGRRVLEEGENLTQYYNKFDETVKAINTGTLVQTPKSSGPTGRQHLRELVNPNIVQTAPQWVDVGGGEAPRRSQVKAAGSEQKAERSRLRLQDRELQEMADGGYCLSMHQPWASLLACGIKRVEGRTWYTSHRGRLWIAAAGKKPSPQEIAQVEAMYRQIYKQDVQFPKEYPTGCLLGCVNVTDCLSQEQFREQYPQISEESASPFVFICTNPQELLVKFPVKGKHKIYVSEETIYKGSWRVRFTKVPRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LEB2</accession>
    <name>A0AAR2LEB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Envelope protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LEB2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081254.1">
        <property type="protein sequence ID" value="ENSPNAP00000072977.1"/>
        <property type="gene ID" value="ENSPNAG00000024151.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064449"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018154">
        <property type="entry name" value="TLV/ENV_coat_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424:SF80">
        <property type="entry name" value="ENVELOPE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424">
        <property type="entry name" value="VIRAL ENVELOPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00429">
        <property type="entry name" value="TLV_coat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58069">
        <property type="entry name" value="Virus ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="651" mass="71961" checksum="3286B96416622F05" modified="2024-11-27" version="1">MNQTLKSKLNKVVYSTGQNWLDALPIVLINHCISKYGGIELDYTLGSSTAWTFDLCDVINCAGQNSTYRGYDVYLCWDLQVTTKCGFGWGWDDRWCRYWHSVIKYTGRWQPTLAARDAYTHKTDWEHMSFQRDFSRTQNPLTLSIKWEKSIMDAHRGRTGRSPYLILGVDVSSKDPLGLIKINMHNKVVSASVSTMTNTFTPATQEVTDPGLKGSDYTKWSPGDIVKLTTGYVGDNLWLKWLAAQAGEAGMEDCVACAQARAPLSLAPAPLFPDTDMTGFKCMIELTKQANPINCTTLADIFPVIANTTGTGPFRAPLSGNFTCFNHTYVDGTGRGKMIGQINPDWCHITYSGNASVGPWAGAGLYYYCGGTTLLVRMPIHTFGLCAMVRLVSPILLVGQRQAKHITMHRRKRETTEDHFDLTKNSLTYIDAIGLVDQIAAGFENIPIISALFPVTPNKNVDRINYVNYQIMRLANLTKVAVEGLSAQLSATSLMAIQNRMALDMLLAETGGVCHMFGDMCCTFIPNNTAPDGSVTKALEGLKALSKEMHDNSGLDNAITGWLDKVFGKWKAVFISLCMSIAVFLAILIICGCCCVPCIRTLAERFITSMFHKQEPKGAIMLPLLDSNGRSGDQDSDLENNMELNRGTIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLB7</accession>
    <name>A0AAR2LLB7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DMAP1-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DIP2A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIP2 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000046243.1">
        <property type="protein sequence ID" value="ENSPNAP00000075131.1"/>
        <property type="gene ID" value="ENSPNAG00000010962.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182997"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05905">
        <property type="entry name" value="Dip2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000003">
        <property type="entry name" value="DIP2 disco-interacting protein 2 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000001">
        <property type="entry name" value="DIP2 disco-interacting protein 2 homolog C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000004">
        <property type="entry name" value="Disco interacting protein 2 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000002">
        <property type="entry name" value="Disco interacting protein 2 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037337">
        <property type="entry name" value="Dip2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF34">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23024">
        <property type="entry name" value="AMP-dom_DIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01137">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51912">
        <property type="entry name" value="DMAP1_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DMAP1-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="49"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1573" mass="171801" checksum="6ACCD3CC620E1B9C" modified="2024-11-27" version="1">AAPRGSPVLYVVIKQLLKGYEKKRGKLLAPYIPQIQGVDPSLQVDHRIQASSQAAPPTSKHNKPRAANSRDERFRSDLHTEAVQAALAKYKERKMPMPSKRRSVLVQSSVEACTPPDTSSASEDEGSLRRQGRMATSSPYQPHPTVEHWFNRVIQGSSTSSSASSTSSHPGGRSHSANATSGSAATVLADLMAHTQLDNHCAPPDVTGLSERSLHAERPQVASVRGLPRGYTHTSSTMETADGVPVNSRVSSKIQQLLNTLKRPKRPPLREFFVDDFEELLDVQQPDPNQPKPEGVQMSPLHGDPLGVVTNWPPSLLASLQRWGTAQPKSPCLTALDNAGKPVYTLTYGTFTLQKLAYTLLNKLSSRNEPLLRPGDRVALVFPNNDPVMFMVAFYGCLLAELVPVPIEVPLTRKDAGSQQIGFLLGSCGVTLALTTDACQKGLPKAQTGEVATFKGWPRLLWFVTDGKHVVKPPKDWSPPIRDASNETAYIEYKTSKEGSTMGITVSHAAMLAHCHALTQACGYTEGETITNVLDFKRDAGLWHGVLMSVMNRMHVISIPYSLMKVNPLSWIQKVHSYKARVAVVKSRDMHWSLLSQKDQRDISLSSLRMLIVADGANPWSISSCDAFLNVFQARGLRPEVICPCASSAEAMTVAIRRPPEMGVPPPGKAVLSMSCLSYGVIRVDTEEKLSVLTLQDVGQVMPGALVCVVRPEGTPYLCRTDEVGEICVSSCTTGISYYGLPGMTKNVFETIPVTASGSPVSDRPFTRSSLLGFIGPDNLLFVVGKMDGLMVVSGRRHNADDVVATALAVEPMKFVYRGRIAVFSVCVLHDERIVVVAEQRPDASEEDSFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKAPLGGIHISETKQRFLEGALHPCNVLMCPHTCVTNLPKPRQKQPGTPVGPASMIVGNLVAGKRIAQACGRDVAQLEDNDQFLYMQDVLQWRAQATPDHPLFLLLNAKGTVASTASCVQLHKRAERVAVALMERGRLNTGDHVALVYPPGTDLIATFYGCLYAGCVPVTVRPPHPQNLATTLPTVKMIVEVSKSVCILTTQAIMKLLRSKEAAAAVDVKTWPMVLDTDDLPRKKLPQIYKPPTPEMLAYLDFSVSTTGILAGVKMSHAATSALCRSIKLQCELYPSRQIAICLDPYCGLGFALWCLCSVYSGHQSILVPPLELESNVSLWLSAVSQFKVRVTFCSYSVMEMCTKGLGSQTEALRVSTCHSLSLLLFGFLPLSSLLCDQLRNVNLSCVRTCMVVAEERPRIALTQSFSKIFKDLGLSPRAVSTTFGCRVNVAICLQGTAGPDPTTVYVDMRALRHDRVRLVERGSPHSLPLMESGKILPGVKVIIANPETKGPLGDSHLGEIWVSSPHNATGYYTVYGEEALHADHFCTKLSFGDTQTVWARTGYLGFLRRTELTDASGERHDALYVVGSLDETLELRGMRYHPIDIETSVIRAHKSIAECAVFTWTNLLVVVVELEGSEQEALDLVALVTNVVLEEHFLIVGVVVVVDPGVIPINSRGEKQRMHLRDGFLADQLDPIYVAYNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLD5</accession>
    <name>A0AAR2LLD5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Nuclease EXOG, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Endonuclease G-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">EXOG</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLD5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041872.1">
        <property type="protein sequence ID" value="ENSPNAP00000077365.1"/>
        <property type="gene ID" value="ENSPNAG00000036224.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108436083"/>
    <dbReference type="GeneTree" id="ENSGT00940000160677"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000003">
        <property type="entry name" value="Nuclease EXOG, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041003">
        <property type="entry name" value="Exog_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF19">
        <property type="entry name" value="NUCLEASE EXOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18026">
        <property type="entry name" value="Exog_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="20">
        <location>
            <begin position="68"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="19">
        <location>
            <begin position="69"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="355" mass="40158" checksum="8B6B544E3256E679" modified="2024-11-27" version="1">MALPRLKFVRFFGGFVCGVTLSTGACVAALRLYLPEQQRDDSGEERESPVQSLIKRYGLPLTGAETRSYTNHILSYDQGRRTPKWVAEHLSTEKLLGRADRRHCKFKPDPGVPELFTAHNEDYLGSGWSRGHMAPAADNKFSQQSMVETFYLSNIVPQNYENNAGFWNRLEMYCRELTERFSDVWVISGPLTLPQVGEDGKKTVSYQLIGKDDVAVPTHLYKVILAQKDPSAKVLALGAFVVPNAPIGFDHHLREYQVRLTELERISGLTFFPKVETQSLKNLCTVDSCELMDFKRFTLYITGRKVGGAKTLAKLDKIMVELKEAGITPDDYLVKLYLEKKQELLQKENPENKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LND5</accession>
    <name>A0AAR2LND5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pleckstrin</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LND5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072889.1">
        <property type="protein sequence ID" value="ENSPNAP00000078070.1"/>
        <property type="gene ID" value="ENSPNAG00000004768.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157885"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13302">
        <property type="entry name" value="PH2_Pleckstrin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000269">
        <property type="entry name" value="Pleckstrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037370">
        <property type="entry name" value="Pleckstrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092">
        <property type="entry name" value="PLECKSTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12092:SF1">
        <property type="entry name" value="PLECKSTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="72"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="5">
        <location>
            <begin position="206"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="314"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="422" mass="47471" checksum="59ADF614ABFEB616" modified="2024-11-27" version="1">MFVIATGQLQSSNSTNSLLNYSPANVSYTTCCFAVCSIVTSWYLSVQQLQRVLWPSPQQCTSTNLGGGVSEEALEQEYLLAISDGTVLNSWKVMWVVLADDGIEFYKKKTDSNPKGMIPLNGATLTSPCQDFNKRTFVFKLSTTKKQDHFFQATHLEERELWVKDIKRAITCLQGGKRFARKSTRKSIRLPESVNLSELYILMKDQDSGIKEMKVEKDKKLFSHCFTGNTVIDWLISQGKVRNRAEGVMLATGLLNEGFLQPAGEISKTAAEGGAESVLLDQHDALYYFADSGFYCEGYSSDEDVIVKEEFRGAIVKQGCLLKQGHRRKNWKVRKFILRDDPAYIHYYDPAKGEDDPLGSIPLRGCVVTAVDYVPDAKRHDVEGNLFEIITSDEVHYYLQAATSEERNEWIKVVQVVAKSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LRS2</accession>
    <name>A0AAR2LRS2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">HIKESHI</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LRS2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064615.1">
        <property type="protein sequence ID" value="ENSPNAP00000077006.1"/>
        <property type="gene ID" value="ENSPNAG00000034733.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000245198"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="1">
        <location>
            <begin position="22"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="119" mass="13299" checksum="458E988D1D0353A3" modified="2024-11-27" version="1">NFMCVQLFKTAVLAPTSPADATPQTTFPVSKFSGDPDQCRGFLLQCSVFFDNSPVATDQAKISFVVSRLTGKALDWATASWSSFSNWTYALYTQRGWSPNLFYYRCGCAFTIMILSFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LVH9</accession>
    <name>A0AAR2LVH9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">ITGB1</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="26">Cell membrane</location>
            <topology evidence="26">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Invadopodium membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Ruffle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 26">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LVH9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071932.1">
        <property type="protein sequence ID" value="ENSPNAP00000078662.1"/>
        <property type="gene ID" value="ENSPNAG00000008014.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286983"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019960">
        <property type="term" value="F:C-X3-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098639">
        <property type="term" value="F:collagen binding involved in cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000043">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000075">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000155">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000003">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.30:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040622">
        <property type="entry name" value="EGF_integrin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033760">
        <property type="entry name" value="Integrin_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF28">
        <property type="entry name" value="INTEGRIN BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18372">
        <property type="entry name" value="I-EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07965">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17205">
        <property type="entry name" value="PSI_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002512">
        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
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        <property type="entry name" value="Integrin_B_tail"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Integrin beta tail domain"/>
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        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="16 26" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="17" id="KW-0965">Cell junction</keyword>
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    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
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    <keyword evidence="8" id="KW-0517">Myogenesis</keyword>
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    <keyword evidence="22" id="KW-0675">Receptor</keyword>
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    <keyword evidence="13" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
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    <feature type="chain" id="PRO_5043714569" description="Integrin beta" evidence="28">
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            <begin position="28"/>
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        <location>
            <begin position="730"/>
            <end position="752"/>
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    </feature>
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            <begin position="29"/>
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            <begin position="753"/>
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        </location>
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    <sequence length="799" mass="88621" checksum="D060CF0246913A9E" modified="2024-11-27" version="1">SNCSSDCQLVFFVLHDEFLLLLLSTEGNECIKANAESCGDCIQVGAKCGWCTDTKFLKQGEPTSARCDDMESLRKRGCVAAKIENPRGSQKTLRNKPVTNRKKGGEKLKPEDITQIQPQKLSLSLRSGEPQTIDLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKNLGTSLMLEMSKITSDFRIGFGSFVEKTVMPYISTTPAKLLNPCTGDQNCTSPFSYKNVLKLTSNGQRFNTLVGQQQISGNLDSPEGGFDAIMQVAVCGEHIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGKCHLENDMYTMSHHYDYPSIAHLVQKLSDNNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVINLIIDAYNSLSSEVILENSKLPEGVTITYQSHCKNGVVSVGEDGRKCSNISIGDEVYFSINITALGCPKQGKTETLKIRPLGFTEEVEIRLSFICDCECQKEGIVRKDLCHNGNGTFECGACRCNKGRIGRECECRKDEVSSEDLDRNCRKDNGTDLCSNNGECVCGKCECKKRDNPEEYYSGKYCECDNFNCDRSNNKLCGGHGRCECRVCICDANYTGSACDCSLDTSTCLASNNQICNGRGLCECGTCRCTDPKFQGPTCEICPTCPGVCTEHKACVQCRAFGTGEKKDTCEQDCNYFTLVKVKDRDKLPQPVQAFPLMHCKERDANDCWFYYTYAVNNYTEVHVVETLECPAGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M079</accession>
    <name>A0AAR2M079_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">WNT3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557919.1">
        <property type="nucleotide sequence ID" value="XM_017702430.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M079"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072825.1">
        <property type="protein sequence ID" value="ENSPNAP00000080394.1"/>
        <property type="gene ID" value="ENSPNAG00000033988.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430137"/>
    <dbReference type="CTD" id="7473"/>
    <dbReference type="GeneTree" id="ENSGT00940000157854"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19335">
        <property type="entry name" value="Wnt_Wnt3_Wnt3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009141">
        <property type="entry name" value="Wnt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF82">
        <property type="entry name" value="PROTO-ONCOGENE WNT-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01843">
        <property type="entry name" value="WNT3PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="355" mass="40075" checksum="ED69C347D87ED245" modified="2024-11-27" version="1">MDLYLVGYIMCMWLSSSRVLGGYPIWWSLALGQQYTSLGSQPILCGSIPGLVPKQLRFCRNYIEIMPSVAEGVKLGIQECQHQFRGRRWNCTTIKDNLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTSTMCGCDSHHKGPPGEGWKWGGCSEDAEFGVLVSREFADARENRPDARSAMNRHNNEAGRMTILENMHLRCKCHGLSGSCEVKTCWWAQPDFRLLGDYLKDKYDSASEMVVEKHRESRGWVETLRAKYAFFKHPTERDLVYYEGSPNFCEPNPETGSFGTRDRVCNVSSHGIEGCDLLCCGRGHNTRTEKRKEKCHCIFHWCCYVSCQECVRVYDVHTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M136</accession>
    <name>A0AAR2M136_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PRELID3B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M136"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059808.1">
        <property type="protein sequence ID" value="ENSPNAP00000080326.1"/>
        <property type="gene ID" value="ENSPNAG00000036327.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182810"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="197" mass="22138" checksum="2736FA3529B03536" modified="2024-11-27" version="1">MLELIFQLWTHIYSTHPWETVTKAAMQKYPNPMNPSVFGVDVLDRTVDQQGRLHSKRLLSTEWGLPSIVKSIIGNARTCTYVQEHSVVDPKEKTLELKSTNITFTNMVSVDERLIYKPHPEEPEKTVLTQEAIISVKGVSLSSYLEGLMANTISTNAGKGREAMEWVIRRLNAEIEELAITARGSIRTPMAAAVTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPB7</accession>
    <name>A0A3B4BPB7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Septin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPB7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001423"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012186.2">
        <property type="protein sequence ID" value="ENSPNAP00000001423.2"/>
        <property type="gene ID" value="ENSPNAG00000007157.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157152"/>
    <dbReference type="OMA" id="IENKEHC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000064">
        <property type="entry name" value="Septin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="10">
        <location>
            <begin position="5"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="8">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR006698-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001423.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="359" mass="41483" checksum="CC4567C458B6628E" modified="2024-11-27" version="2">KSVKKGFDFTLMVAGESGLGKSTLVNSLFLTDLYKDRKLLNAEERITQTVEITKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKSVADYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHEKVNIVPVLAKADTLTPSEVKKKKIKIREEIEQYGIKIYQFPDCDSDEDEDFKQQDQELKDSIPFAVIGSNTVVEAKGKRVRGRLYPWGIVEVENSAHCDFVKLRNMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRMVVKERNRNKLTRESGTDFPIPMVPGVADTETEKLIREKDEEVQHPKTLNLTYNRIQWAAVDACVIFHSIIFFTKYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQA2</accession>
    <name>A0A3B4BQA2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STRA8 bHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037395820.1">
        <property type="nucleotide sequence ID" value="XM_037539923.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037395823.1">
        <property type="nucleotide sequence ID" value="XM_037539926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQA2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000604"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013567.2">
        <property type="protein sequence ID" value="ENSPNAP00000000604.1"/>
        <property type="gene ID" value="ENSPNAG00000007617.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425354"/>
    <dbReference type="GeneTree" id="ENSGT00390000017181"/>
    <dbReference type="OMA" id="YMQIIEF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090427">
        <property type="term" value="P:activation of meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071300">
        <property type="term" value="P:cellular response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057021">
        <property type="entry name" value="bHLH_STRA8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033537">
        <property type="entry name" value="Stra8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35254">
        <property type="entry name" value="STIMULATED BY RETINOIC ACID GENE 8 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35254:SF1">
        <property type="entry name" value="STIMULATED BY RETINOIC ACID GENE 8 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23175">
        <property type="entry name" value="bHLH_STRA8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STRA8 bHLH" evidence="1">
        <location>
            <begin position="22"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="333" mass="37272" checksum="519A2265618981E2" modified="2018-12-05" version="1">MSCRGVVHCNEQGKKREAASGHRSVQGTALQACQRVTLADLFESLRNVVCPSTQKTPAKWKILDHAKGFLLEKEAYLSKLLALKAVFLQDDGGPKSLEEVREQYKRLYSKCFLHTCRCRHRIHVPDVDQGPCSNEEDSADEEVDVALQSQSSGLSAYSIQEFEGYLFLYRQTLELLLRSGILSPDQTGLSMVSEAISGLWNSLPPEQRAAVQQCTLKESAVTWIEPANNTPCSSLDSSQLTNLNASGTAFEEDLLQDAYDVVQRDMDDTTANSPSLVPPLNGDNEKLSEIYKDVMGFVRSHMSEDQKLTQDMYLTADYEELFLRCSESFDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRU1</accession>
    <name>A0A3B4BRU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-proliferative protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BTG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560205.1">
        <property type="nucleotide sequence ID" value="XM_017704716.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRU1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001300"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012417.2">
        <property type="protein sequence ID" value="ENSPNAP00000001300.1"/>
        <property type="gene ID" value="ENSPNAG00000008076.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431510"/>
    <dbReference type="GeneTree" id="ENSGT00950000182952"/>
    <dbReference type="OMA" id="WFPLMPS"/>
    <dbReference type="OrthoDB" id="19928at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.90:FF:000003">
        <property type="entry name" value="BTG1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.90">
        <property type="entry name" value="Anti-proliferative protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002087">
        <property type="entry name" value="Anti_prolifrtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033332">
        <property type="entry name" value="BTG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036054">
        <property type="entry name" value="BTG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22978:SF40">
        <property type="entry name" value="ANTI-PROLIFERATIVE PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22978">
        <property type="entry name" value="B-CELL TRANSLOCATION GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07742">
        <property type="entry name" value="BTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00310">
        <property type="entry name" value="ANTIPRLFBTG1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00099">
        <property type="entry name" value="btg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160696">
        <property type="entry name" value="BTG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01203">
        <property type="entry name" value="BTG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anti-proliferative protein" evidence="2">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="153" mass="17212" checksum="2BD5E27D7C11026E" modified="2018-12-05" version="1">MKTEVSTAVNFISKLLRGTGLLSEEQLHHFSLSLEEALGEHYQHHWFPDAPCRGSGYRCIRINHKMDPLIGKAALTIGLTREQLFSLLPSELTMWVDPYEVSYRIGEDGSICVLYESTPPPKMDPSPADSCKEELRIVRSSPSKPFNMMTVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BS82</accession>
    <name>A0A3B4BS82_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Myocyte enhancer factor 2ca</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BS82"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002490"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010331.2">
        <property type="protein sequence ID" value="ENSPNAP00000002503.2"/>
        <property type="gene ID" value="ENSPNAG00000008875.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157492"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022102">
        <property type="entry name" value="HJURP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF23">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="377"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002503.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="484" mass="52360" checksum="1FF2297188F1CAC6" modified="2024-11-27" version="1">MGRKKIQIARIMDERNRHVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVETLRKKGLNGCDSPDIDADDSGHSPESEDKYRKINEDIDLMISRQRLCTIPPSNYDMPVSIPISNPNSLIYSHPGGSLGNPNLLPLAHPSLPRNTLSPGVTHRPPSVGNTGTVSTAYLFSPISITPIGNGYGNHRNSPGLLVSPGSMSKGMQAKTPPPMSINMSNRKPDLRVLIPPGAKNTMPSINQRINNSQSAQSLTTPVVSVATPTLPGQGMGGYPSAISASYGTEYSLSSADLTSLSGFNSSSSLHMGSMTGWQQQHLQNMQHPALGHLGNCISTQLCQSSNLSLPSTQSMTIKSEPVSPPRDRTGSGCGGYAQPPNPPSQQQVPPPAQQSLLRQDSGRSPVDSLSSCGSSYEGSDRDEHRADFHSPLGLLRPPSHEESESPSVKRVRLAEGWAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTF2</accession>
    <name>A0A3B4BTF2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Urotensin-2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Urotensin II receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">UTS2R</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">High affinity receptor for urotensin-2 and urotensin-2B. The activity of this receptor is mediated by a G-protein that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BTF2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001799"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011531.2">
        <property type="protein sequence ID" value="ENSPNAP00000001799.1"/>
        <property type="gene ID" value="ENSPNAG00000000942.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278323"/>
    <dbReference type="OMA" id="PMMLAIR"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001604">
        <property type="term" value="F:urotensin II receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097746">
        <property type="term" value="P:blood vessel diameter maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14999">
        <property type="entry name" value="7tmA_UII-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000183">
        <property type="entry name" value="Urotensin-2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000670">
        <property type="entry name" value="Urot_II_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230:SF60">
        <property type="entry name" value="UROTENSIN-2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00647">
        <property type="entry name" value="UROTENSIN2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="380" mass="43275" checksum="8B317FBC507E7533" modified="2018-12-05" version="1">MLNHSLLGATQSVGIKQEMTTVSVESLVAMVDRIPNATETPVEASAEDMAATFTIGTILSLVCLVGVSGNIYTLVVMCHSMRSAASMYIYIVNLAMADLLYLLTIPFVVCTHFLKGWYFGDVGCRTLISMDFLTMHASIFTLTVMSTERYFAVLKPLDTVKRSKSYRKAIALLVWTASLILTLPMIIDVELKTENSKLMCQTTLSPLSYKIYISSLFCTSIVAPGVIIGYLYICLARTYWISQTETFKQTQKLPNHKVLYLIFTIVLLFWACFLPFWIWQLLGQFKPEMVLSTKARRNINYLTTCLTYSNSCINPFLYTLLTKNYKEYLRKRQRTWTAGSYFNRRNRFQRSPRRSLSSSSQQCTESFVLTHTSRSNNSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUH5</accession>
    <name>A0A3B4BUH5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TOPBP1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the TOPBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564732.2">
        <property type="nucleotide sequence ID" value="XM_017709243.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000003292"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008981.2">
        <property type="protein sequence ID" value="ENSPNAP00000003292.2"/>
        <property type="gene ID" value="ENSPNAG00000009700.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434243"/>
    <dbReference type="GeneTree" id="ENSGT00940000157001"/>
    <dbReference type="OMA" id="NVHCLKT"/>
    <dbReference type="OrthoDB" id="251770at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902165">
        <property type="term" value="P:regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17737">
        <property type="entry name" value="BRCT_TopBP1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17731">
        <property type="entry name" value="BRCT_TopBP1_rpt2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17718">
        <property type="entry name" value="BRCT_TopBP1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17749">
        <property type="entry name" value="BRCT_TopBP1_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18434">
        <property type="entry name" value="BRCT_TopBP1_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17727">
        <property type="entry name" value="BRCT_TopBP1_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17738">
        <property type="entry name" value="BRCT_TopBP1_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17728">
        <property type="entry name" value="BRCT_TopBP1_rpt8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000018">
        <property type="entry name" value="DNA topoisomerase 2-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000028">
        <property type="entry name" value="DNA topoisomerase 2-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000010">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000020">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000021">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000023">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000029">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059215">
        <property type="entry name" value="BRCT2_TopBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049542">
        <property type="entry name" value="TopBP1-like_BRCT0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044737">
        <property type="entry name" value="TopBP1_BRCT_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049936">
        <property type="entry name" value="TopBP1_BRCT_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561">
        <property type="entry name" value="DNA REPLICATION REGULATOR DPB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 2-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21298">
        <property type="entry name" value="TopBP1_BRCT0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="102"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="195"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="357"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="631"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="909"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="1282"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="1395"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="450"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="508"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="791"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1049"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1097"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1176"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1225"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1490"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003292.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1530" mass="168750" checksum="E453DF3BCCFFED3C" modified="2024-11-27" version="2">MSKGSQEAYLVKFVEHGGQKNQLALKAYEAIVGLQSEKYVQTVDEEAALDLEHNDKSLYVFSDFTSSAFEHCRKLGCRIVSPLVVLFCLQQQRCVPKAEQPVYNMAMADVTVSCTSLDKEARTEVMDLVQLMGGRVYRDLNVSVTHLVAGEVGSKKYVVAANLGKPVVLPSWVKACWEKSQDSLFRYTDLRLEDYLCPVLKGCTVCVTGLSTVERKEVQRLCEQHGGRYTGQLKMNECTHLIVSEPSGQKYEFARKWNVYCISVHWLFDSVEKGFCQDESRYTVERGDKKRGEKPDRPNTSTPTGSSKSKEDGPSLLGLSHISNISLNVNETALTTTTMSRIEPPDPIDTFDITVCPADDLLDGCKLYLCGVVGKRLEKLRRLVNSAGGLRFNQLGEELTHIVMGEPDQDVKNFLAKATHRPHVVTVQWLLDSFSRGSLLPAEGYFHPSFPPPAPAPEDMPALRPVAPRSSRPSTALPPAPSPNLQAKAEEDLLSQYMDNDQTAVELPAAPNNNYTTHSSVLPDPGPPGKDSTLAEASEGGLFRGKRFLLVGFGQEAEDQLSVLVMENAGKVLVGRFRGVADYAIVPLLGCEVEATVDEVATDTWLSMCVEHQCLLPLASHPLFTPVAVKEGYSPLRDCVLSVSQFTGAERESLIQLAKHLGASVQDYFVRAANQRKGMLASTHLVLQTPEGTKYQAAQKWGLPAVTLRWVLESARTGKRPDEARYLVELPPSPERTEGSFIGASQKIPAAPVSLRLSPELPLLPPQSSAAVTPLDTGRLQSRAVHSVLRKLNKGKEEEAEATTQAQDKGKGSNQKEGCLQLDTPSRFLSRDRLFGPSFNTTDVCDHLKTPGNKPQQGQRMETPLSEVIERNLKAAVANSNRSHIMSLAALTASPQLDKELEPKTEEKVPAAPLTGVVVCVSRKLNKKQSELNVMAASLGADFRWACDESVTHYIYQGKVGDNTKEYRAVKERGLHIVSQHWLQACAEEQKRVSESLYPFTFNPKMSLTLSQVTDSTQKSPPLSTLRTKLRTLTEQEDTWKGPVDSMTEIATPQRGSLVEPEGEQQPSSTENRDLTETLEMRENLQRQLQEIMSATKLTSGRRASSRLARVGSGGMDSPCTPESLGRVGRRSRTLEALRMSRQVAMDINTEASQSEQIVWDDPTAREERAKLADNLQWPGSPSQHSEPLAILPPPHTGEPQHNRDSMTDSELVEMAACEVIEEQMKQKVTPPRSNVDQRVPKTPEAPSIAFPISKPAVPPEPQIEEEETEKRKQVPPRFQLSSLSPQERIDYSHLIEVLGGIVLEKQCFDPSCSHIIVGFPLRNEKYLAAMAAGKWILHRSYLEACRAEGHFLPEENFEWGSRSILDAVPSISSQQRRLAQAALRWRKALQGRAEKEGAFGGWTVMLNIDHARDAGFRRLLQSGGATVLPAPSPSLYKDTTHLFADFSRLGPGDVRVDVAEATACGVSCLRPEYIADYLMQDPSPPMDAYYLPGAAPSGLPEGLDKGQPTPSRKRKASGDASSLKKSRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVP5</accession>
    <name>A0A3B4BVP5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVP5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010099.2">
        <property type="protein sequence ID" value="ENSPNAP00000002651.2"/>
        <property type="gene ID" value="ENSPNAG00000008983.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182912"/>
    <dbReference type="OMA" id="CKWESIF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF25">
        <property type="entry name" value="ZINC FINGER (C2H2)-60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002651.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="91" mass="10092" checksum="DAD15437B1834EC3" modified="2025-10-08" version="3">MAAETMAVFLVRDESGQVGNVGIVIEGSIVLDHLGDLSRGCCYLLGLTYALDLKYPKTLKYIFEVFQKILLEVDPGKLSTKVLRLKNNLMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVV8</accession>
    <name>A0A3B4BVV8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Interleukin-12 subunit alpha</fullName>
            <shortName evidence="1">IL-12A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">IL12A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IL-6 superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVV8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009959.2">
        <property type="protein sequence ID" value="ENSPNAP00000002716.1"/>
        <property type="gene ID" value="ENSPNAG00000009080.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016906"/>
    <dbReference type="OMA" id="RIDLEHY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005143">
        <property type="term" value="F:interleukin-12 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043330">
        <property type="term" value="P:response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032496">
        <property type="term" value="P:response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004281">
        <property type="entry name" value="IL-12_alpha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03039">
        <property type="entry name" value="IL12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0202">Cytokine</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0339">Growth factor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0964">Secreted</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017102438" description="Interleukin-12 subunit alpha" evidence="1">
        <location>
            <begin position="17"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="186" mass="20693" checksum="2EC2BF22932AEEBD" modified="2018-12-05" version="1">ALCLVTVALLCGGALANPVPSSAPPGPDAALCTRHARSLLFGLRRALEERRGSGALFHGFNCTELRAELRPDSQTVAVCQPDQYVCIVLTLVSPQSECLENIRGDLQHYKLMLRDYVKTLDQKQKSQSITELTSITSLIQTLQETWVATDPFYDRMDLCKWLKGLHIRTVTINRVLGYISAGEYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXC2</accession>
    <name>A0A3B4BXC2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Metastasis associated 1 family, member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MTA3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the metastasis-associated protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558538.1">
        <property type="nucleotide sequence ID" value="XM_017703049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BXC2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003201"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009129.2">
        <property type="protein sequence ID" value="ENSPNAP00000003201.1"/>
        <property type="gene ID" value="ENSPNAG00000009661.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430512"/>
    <dbReference type="CTD" id="57504"/>
    <dbReference type="GeneTree" id="ENSGT01030000234573"/>
    <dbReference type="OrthoDB" id="2193595at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04709">
        <property type="entry name" value="BAH_MTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11661">
        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000012">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000001">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000001">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040138">
        <property type="entry name" value="MIER/MTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035170">
        <property type="entry name" value="MTA1_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
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        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN AND MESODERM INDUCTION EARLY RESPONSE PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN MTA3"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="4"/>
            <end position="147"/>
        </location>
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        </source>
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    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="708" mass="80252" checksum="FBAB1AA785502321" modified="2018-12-05" version="1">MAANMYRVGDYVFFENSSSNPYLIRRIEELNKTASGNVEAKVVCFYRRRDISQSLIQLADKHAKDLEEEKESPSEPELSEKQKHQLRHRELFLSRQYESLPATHIRGKCSVALLNETESVLSYLDKEDTFFYSLVYDPTQKTLLADKGEIRVGPRFQADVPDMLPEGEADDRDQSKLEVKMWDPECPLTNKQIDQFLVVARAVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLHRHGYDLSSALSVLVPQGGPVLCRDEMEEWSSSEANLFEEALEKYGKDFNDIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESKLKQVYIPTYNKPNPNQISVSNGKMATMNGAAGPGGFHTAGGGRACESCFAMQSPQWYSWGPPNMQCRLCVSCWMYWKKYGGLKMPSRAEGAEEKTPPSPASNELRSHGHTLRQTSHMVPVRNSGSPKSSMKTKQAFLLQATRLTKLARHMCRDLIRLRRAARRPFVPINCGAIKAEYMIRAAEGMTGRSTKPKPSQRSTLTSILQYLESRPVPHVQRSHRTPGLQVQPPRRLLSSLPSHGPLSMLGKRSYHHHSRVESTERRGNAPGQENQGHIVAPILQHNGRSASGPSLRPSNLMLRKRRPNWIDAPDDSFFLVSRETRKARRLLSRSQLRRACRQPCEQINLRRVPQGPAQGPVLVPPHPSLRMRGPIVIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7B2</accession>
    <name>A0A3B4C7B2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Contactin 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CNTN2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. Contactin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550164.1">
        <property type="nucleotide sequence ID" value="XM_017694675.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000007015"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002546.2">
        <property type="protein sequence ID" value="ENSPNAP00000007015.1"/>
        <property type="gene ID" value="ENSPNAG00000002475.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425759"/>
    <dbReference type="CTD" id="6900"/>
    <dbReference type="GeneTree" id="ENSGT00940000164703"/>
    <dbReference type="OMA" id="MPDANPR"/>
    <dbReference type="OrthoDB" id="6418794at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
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        <property type="match status" value="4"/>
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        <property type="entry name" value="Ig5_Contactin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="contactin-3 isoform X1"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF28">
        <property type="entry name" value="CONTACTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017294621" description="Contactin 2" evidence="14">
        <location>
            <begin position="21"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="32"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
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            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
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            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="326"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="416"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="508"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="609"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="712"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="814"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="695"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="895"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="697"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009812"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1041" mass="114096" checksum="50378F468CCD2F75" modified="2018-12-05" version="1">MRILLFLLAFSSLAPRHTAGGEVCVSGHDSGPVFEEQPSSLIYPEGLPEGKVTLNCQARASPAASYRWRVNGSDVVIREDSHYTLVAGNLVISNPQHGRDSGSYQCFAINRCGTIVSRAANLKFGYLRDFPSESRSPQTAYEGAGTFLACQAPAHYPALSYRWILNEFPSFVQPDGGRWFVSQVTGNLYLANARANDTGSYFCLTTINMDISTKSTFSKAIQLTVQPDANPRKSAPNIRVRFPSETYALAGHTTQLECFAYGNPTPKLRWRKVDGVLPSKVGASADGPILTIPDLSFDDEGMYECEAYSSEGRDTHQGRVSVQAQPEWLQVMSDSEVEISSELHWSCVAAGKPRLIIRWLRNGMPLGTQDRVEVNNGLLKISNLALEDSGMYQCVAENKHGTIYSNAELRVQVQAPDFRLNPVRKLVPAARGGQVKMECKPRAAPKPTLFWSRGTELLTNSSRVTVTPDGVLWIYNISRADEGKYTCFAENYLGKANSTGHLSVRDATKIKLAPSNADINQGENVTLQCHASHDPTMDLTFTWSLNGVLLDLEEPNGHYHRVEGKETIGDLAIVNAQLSQAGIYTCSAQTVVDSAVASARLVVRGPPGPPGGLVVKSVNETAVELRWSRGYDNHSPIGKYVIMGRSEHSHDWKRMRTDPVNIEGNAESARVIDLRPWMDYEFQVIASNILGSGEPSMPSQSIRTKQSAPTVAPSGLGGGGGDRNELIITWTPMAREYQNGDGFGYILWFRKLETPSWTVVRVPNAESSRYVYSNQSLSPYCPFEVKIKAYNSKGEGPFSQIAVVHSAEEEPTVSPRKINATAVTAFEIQVSWEPVQHLSTNGILRGYEIRYWRQHEREAAADRVRTAGLENTARVSGLRPNTLYHVTVLAYNSAGTGPPSPRTTVITKKPPPNRPPGNVSWKTDGSWVTVRWDHVKSMDNESTVQGYKVLYKHEGQSALKVLDKGKTSVNLPLPKDNGYVVLEIRSWGEGGDGAAHEIIVSRDSGTGMMVQNNSAGISKSSCTSVLVSTTAVLTLIGLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7S2</accession>
    <name>A0A3B4C7S2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger protein 521</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ZNF423</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C7S2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006966"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002629.2">
        <property type="protein sequence ID" value="ENSPNAP00000006966.2"/>
        <property type="gene ID" value="ENSPNAG00000002445.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159287"/>
    <dbReference type="OMA" id="CDRTFPR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000244">
        <property type="entry name" value="zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000483">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000548">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000107">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000143">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000167">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000261">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000326">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000906">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000998">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF128">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="270"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="308"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="466"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="542"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="661"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="691"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="721"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="749"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="779"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="810"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="836"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="923"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="967"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="996"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="1206"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="1293"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="1324"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="1354"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="386"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="892"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1148"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1227"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1151"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1185"/>
            <end position="1203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006966.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1379" mass="153768" checksum="631C54B2023B5A70" modified="2024-11-27" version="2">MSRRKQAKPRSLKVEDNVTEDQQTPGQAAILSDPECAPERVSEDAEDGRLRRRRCSPEEGDEAEEEDEEHSCRACRQVFDSLSDLTQHKINQCQLTDGADLEDDPSCSWPASSPSSKDQTSPAHGDDYDFGEDEGGPGLPYPCQFCDKSFSRLSFLKRHEQSHSDKLPFSCTFCSRLFKHKRSRDRHVKLHTGDKKYHCGECDSAFSRSDHLKIHMKTHAANKPHKCPVCRRGFLSSSSLHGHMQVHERGKDAQGTGLSRMEEWKLKETRKCSRCEEGFDVPEELQKHIAECHPECSPPEEGSLGPGLQCIYCHEPFSDEGTLLSHIDQAHGRDRKGNTCTVCSEHFATVEELYAHMDVHQLPESSNHSNSPSLLTVGYTSVSSTTPDSNLSVDSSTMVDSAPTVPKARGRRKRAAQHATDICGRPAKQPKVAYSCIYCNKQVFSSLAVLQIHLKTMHVDKPEQAHTCQFCLEVLPSLFNLNEHLKQVHNAEDPSALLASLSDALLQCNFCPEMLGDLNALQEHIRCSHGFPSPVAKESNAFFCPQCFMGFLTEATLEEHVRQTHCDSGSLRFDSPLAVTPKDPIVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYINNGRRTHRALSPSSPISVEQATLLKQGSTSSRSASEFICNQCGAKYTSLDLFQTHLKTHLDCGLPQLSCPQCNKDFPNQEALLKHVTVHFTITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSTQLHLAVKHSNEKKIYRCTSCNWDFRHETDLQVHVKHSHLENQGRVHRCIFCGESFGTEVELQCHITTHSKKYNCRFCNKAFHAIILLERHLREKHCVFEGKAQNCGTNGSTSGGGDSVAKDETDLQGILTNSHGGAGTGESHNSHDGSEEDVDSSEVMYSCDICGASYTMESLLTNHQLRDHNIRPGESAIHKKKAEMIKGSHKCNVCSRTFFSEAGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGSCRICKLPLQSEEDFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGAMSTNHPSGRSAITHHQHHQSQKLFKCASCLKEFRSKQDLVKLDINGLPYGLCASCVSAAGSKSSSPTINGGKQQQHGVQTPTVSAVGWSHGESLSPGGVKAKSTSYSTSSSSSTSSTAAKTRCSSCNVKFESEAELQTHLSTVHRDQTGEGSGGQLRTPQGSPMPRVSPTQSEEKKTYQCIKCQMVFHSEWDIQVHVANHMIEEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQASKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTLSQHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C866</accession>
    <name>A0A3B4C866_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567456.1">
        <property type="nucleotide sequence ID" value="XM_017711967.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C866"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007370"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001948.2">
        <property type="protein sequence ID" value="ENSPNAP00000007370.1"/>
        <property type="gene ID" value="ENSPNAG00000013210.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435855"/>
    <dbReference type="CTD" id="553052"/>
    <dbReference type="GeneTree" id="ENSGT00940000163566"/>
    <dbReference type="OMA" id="VHIMDTS"/>
    <dbReference type="OrthoDB" id="265044at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04147">
        <property type="entry name" value="Ras_dva"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000475">
        <property type="entry name" value="GTP-binding protein Rhes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF9">
        <property type="entry name" value="SMALL GTPASE RAS-DVA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="208" mass="23411" checksum="A28CF55831FFDDA1" modified="2018-12-05" version="1">MSLDVKEKTEVRLVFMGAAGVGKTALIQRFLQDSFEPKHRRTVEELHSKEYEVGGIKVTIHIMDTSGSYSFPAMRKLSIQNGDAFALVYSVDDPESLDAVKSLREEILEVKEDKFTPIVVVGNKTDRQGERRVSAEDVLATVELDWNHCFLEASAKENNNVLEVFRELLQQANLPSRLSPALCRRRETFPKNIGARPPMNKTNSCTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8E8</accession>
    <name>A0A3B4C8E8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">C1orf53</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8E8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001010.2">
        <property type="protein sequence ID" value="ENSPNAP00000007933.1"/>
        <property type="gene ID" value="ENSPNAG00000013602.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016548"/>
    <dbReference type="OMA" id="IIMSARA"/>
    <dbReference type="OrthoDB" id="274765at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040807">
        <property type="entry name" value="DUF5522"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L14, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037:SF2">
        <property type="entry name" value="SIMILAR TO NOVEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17653">
        <property type="entry name" value="DUF5522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="152" mass="16924" checksum="C2CBDBA78076B740" modified="2018-12-05" version="1">MLLLSGLCRSSWTLRSSPKLGRAPIIMSARARSACSSPGEEGDTLTCSRHNTLEDEARTEERGVGKAAGTLQLLEENRLSEQERVIHRLHQEACKAGERTYIDPLSGYKVFTEFAHKQRGRCCGSACRHCPYGQINVEDSSKKKTFNSAFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8X3</accession>
    <name>A0A3B4C8X3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plasma retinol-binding protein II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RBP4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">RBP delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8X3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007351"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001986.2">
        <property type="protein sequence ID" value="ENSPNAP00000007351.1"/>
        <property type="gene ID" value="ENSPNAG00000002608.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047107"/>
    <dbReference type="OMA" id="KYWGMAS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060417">
        <property type="term" value="C:yolk"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034632">
        <property type="term" value="F:retinol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032526">
        <property type="term" value="P:response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000004">
        <property type="entry name" value="Retinol-binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022271">
        <property type="entry name" value="Lipocalin_ApoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002449">
        <property type="entry name" value="Retinol-bd/Purpurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873:SF2">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036893">
        <property type="entry name" value="Lipocalin_ApoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500204">
        <property type="entry name" value="RBP_purpurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01174">
        <property type="entry name" value="RETINOLBNDNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="14">
        <location>
            <begin position="58"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="41"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="107"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036893-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036893-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="212" mass="24030" checksum="F9E9F8CE8398E37C" modified="2018-12-05" version="1">MQRTRTQELANLVGGEGTVDSNMLRFFMAFCVLATGWAQECVVDQIPVKEGFDKYRFQGTWYAVAKKDPVGLFLLDNVVASYKVEDDGKMTATAVGRVIILNNWETCANMFGSLEDTSDPAKFKLRYWGAAAYLQSGYDDHWIIDTDYDNYAIHYSCRALDADGTCLDGYSFIFSRHPTGLRPEDQKIVTQKKQEMCFLGKYRRVAHNGFCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CBH3</accession>
    <name>A0A3B4CBH3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CBH3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008630"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014759.2">
        <property type="protein sequence ID" value="ENSPNAP00000008630.1"/>
        <property type="gene ID" value="ENSPNAG00000014232.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234921"/>
    <dbReference type="OMA" id="MESTWPR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="902" mass="99241" checksum="09BA1D74D1E211EA" modified="2018-12-05" version="1">MGKPLSRPGCLRQSPCCSKKNDREGYNEDGYIPQRSIYDTMCINEQIDHSSAHSTLGSRRGRETDFSSNGSLGGDMFDTRSSVLLSSGRKLDERFIFDSLKLANDELRSPGGFVRSTSPSVSCSSAPGGSMNKRHPHQDSGKKDNLCRHSWKVLTPPKFPDALELSPGEKNYLNPGVAFFPNSMTSPQISPFSGSPFSTGTHTPSVFFSPSPLPYHQQFHRQSLPTQPSSPRPPLSELSVSALMCGQDMQGERLEQENRGYGNVVKERVFETFKPRPIEPIESVESVNEPQPTTPEPENDTTPLLHSQISKAPKICTTSMESTWPRRLIGRRRTVRQGGAIHNLPILPPLPSLLIRSVSDKKPMPRLSPFPEHQGKVEGQLGKSMLKSELKDCSLQDWKILRQIEEKKAGSSKIEEEKEEVMEGSSKMAPVQEKAEETPEENTASECEVENWEAVLEMVNSLWDETWKDSDSQNLGSLRRWPLLHPPTGFGGSQAPSGTSSELGAEDLLELERISRGDSLDSELNDLLLSQSDFQSKENNVIVMQPTGENLKKAMVPTLLDLGAAEAQLGNKYAHTGSPDSNLTLDSDSSGIYMSNPSQTSKEDVTSDGDRSMFELDLGNATSLNKSENNVTRTRPPEVNDSKVSTSDEKLRAESKEVKTKGTVMQGSKSMDEGSRDQKIRNTVNTSKTDVPMPLSPSKYEVKRALLDPGDMDPFVATDSFVYLAVSVLPPPLQEVVPTPPREEPTPLPSFTKPCPYPDECDFLSTDSFVYLAAPDCHLLGTEGHSVYGDSVDSEDSGSRADFVLASTVGDSDWDSDLSDSDAEPGSSKPAWDHWEELEQDVLQGLFCEDQSERETECHDNQAQQISGQRGELAAIVVQERVEVCSQHTQGGELLAEVKSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CC27</accession>
    <name>A0A3B4CC27_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RS1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CC27"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008004"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000901.2">
        <property type="protein sequence ID" value="ENSPNAP00000008004.1"/>
        <property type="gene ID" value="ENSPNAG00000013654.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157334"/>
    <dbReference type="OMA" id="VCKCDCQ"/>
    <dbReference type="OrthoDB" id="9973968at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF5">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017362237" description="F5/8 type C domain-containing protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="8">
        <location>
            <begin position="71"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="232" mass="26371" checksum="9A266886BD1BA82C" modified="2018-12-05" version="1">MEHSSLHALLLTLLLIGEARIGVHSQQGGEDNESWTKACKCDCDGGESPTEFTSITTGTSWSRGLECMPECPYHRPLGFEAGAVTPDQISCSNEDQYTGWFSSWTPNKARLNNQGFGCAWLSKYQDTNQWLQIDLKEARVVSGILTQGRCDADEWITKYSVQYRTNENLNWIYYKDQTGNNRVFYGNSDRSSSVQNLLRPPIVARYIRIIPLGWHTRIAIRMELLLCMNKCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEN4</accession>
    <name>A0A3B4CEN4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GPN3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Small GTPase required for proper localization of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017543093.1">
        <property type="nucleotide sequence ID" value="XM_017687604.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEN4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010412"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035710.2">
        <property type="protein sequence ID" value="ENSPNAP00000010412.1"/>
        <property type="gene ID" value="ENSPNAG00000015979.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414710"/>
    <dbReference type="CTD" id="51184"/>
    <dbReference type="GeneTree" id="ENSGT00950000183172"/>
    <dbReference type="OMA" id="LYTHMTV"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000616">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="285" mass="32816" checksum="874D533EF82A4E93" modified="2018-12-05" version="1">MPRYAQLVMGPAGSGKSTYCSTMIQHAEAINRSVQVVNLDPAAEHFNYPVMADIRELIQVDDVMEDDTLRFGPNGGLVFCMEYFASNFDWLEESLGHVEDDYILFDCPGQIELYTHLPVMKQLVEQLQQWEFRVCGVFLVDSQFMVESFKFISGVMAALSAMVSLEIPQVNIMTKMDLLNPKAKKEIEKYLDPDMYSMMEDSSISLRSQKFKKLTKAICGLIDDYSMVRFLPFDRTDEEGINIVLQHIDFSIQYGEDLEFKEPKEVEEEPSNTNYDEFFQDRVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CHP1</accession>
    <name>A0A3B4CHP1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bardet-Biedl syndrome 2 protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Cilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytoskeleton</location>
            <location evidence="6">Microtubule organizing center</location>
            <location evidence="6">Centrosome</location>
            <location evidence="6">Centriolar satellite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017546575.1">
        <property type="nucleotide sequence ID" value="XM_017691086.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017546584.1">
        <property type="nucleotide sequence ID" value="XM_017691095.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017546593.1">
        <property type="nucleotide sequence ID" value="XM_017691104.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CHP1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010958"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017771.2">
        <property type="protein sequence ID" value="ENSPNAP00000010958.1"/>
        <property type="gene ID" value="ENSPNAG00000005140.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423647"/>
    <dbReference type="CTD" id="583"/>
    <dbReference type="GeneTree" id="ENSGT00390000017113"/>
    <dbReference type="OMA" id="MSDGANC"/>
    <dbReference type="OrthoDB" id="2120021at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070121">
        <property type="term" value="P:Kupffer's vesicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032402">
        <property type="term" value="P:melanosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051877">
        <property type="term" value="P:pigment granule aggregation in cell center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042478">
        <property type="term" value="P:regulation of eye photoreceptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000967">
        <property type="entry name" value="Bardet-Biedl syndrome 2 protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016616">
        <property type="entry name" value="Bardet-Biedl_syndrome_2_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055381">
        <property type="entry name" value="BBS2_CtH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029333">
        <property type="entry name" value="BBS2_GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055380">
        <property type="entry name" value="BBS2_hp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029429">
        <property type="entry name" value="BBS2_Mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029430">
        <property type="entry name" value="BBS2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055379">
        <property type="entry name" value="BBS2_pf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32465">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32465:SF0">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23351">
        <property type="entry name" value="BBS2_CtH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14782">
        <property type="entry name" value="BBS2_GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23353">
        <property type="entry name" value="BBS2_hp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14783">
        <property type="entry name" value="BBS2_Mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14781">
        <property type="entry name" value="BBS2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23350">
        <property type="entry name" value="BBS2_pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013684">
        <property type="entry name" value="BBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3 6" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ciliary BBSome complex subunit 2 N-terminal" evidence="8">
        <location>
            <begin position="20"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Ciliary BBSome complex subunit 2 middle region" evidence="10">
        <location>
            <begin position="165"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="BBS2 GAE" evidence="9">
        <location>
            <begin position="394"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="BBS2 platform" evidence="11">
        <location>
            <begin position="485"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="BBS2 hairpin" evidence="13">
        <location>
            <begin position="581"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="BBS2 C-terminal helix bundle" evidence="12">
        <location>
            <begin position="683"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR013684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="715" mass="78930" checksum="5290A57898470C15" modified="2018-12-05" version="1">MLVPIFTLKLNHKINPRMVTVGKFDGIHPCLTAATQAGKVFIHNPHSRGQRQTAHRLSQSTQDSDISLLNINQAVSCLTAGTLGPNTTGDTLLVGSQTNLLAYDVHDNADIFYREVTDGANAIVLGKLGDIQSPLAIIGGNCALQGFDYEGNDQFWTVTGDNVRSLVLCDFTGDGKNELLVGSEDFDIRVFREDELVSEMAENETVTSLCHMHGSRFGYALANGTVGVYDRTARYWRIKSKNHAMSIHAFDLNADGVVELITGWSNGKIDARSDRTGEVIFKDNFSSSVAGVVEGDYRMDGQIQLICTSVEGEVRGYLPASKEMKGNLMDASVEQDLIRELSQRKQNLLLELRNYEENAKAVSGASEGENQMGVIPANTQLQTALSVRAATESQRAHIELSISTPNETIIRAVLIFAEGIFEGESHVVHPNAQNLSGCIYVPIIPPKDIPVDLHIKAFVGGRISTQFHVFEITRQLPRFSMYDLHVDPSAPRPTGRVTFSVNDRPQRVVMWLNQNFLLPEDINSPDVTFTSLRGGGLLTISMRSSGEITLSTDDIDLAGDLVQSLASFLAIEDLQAEADFPTYFEELRVTLTEVDEFHSVHQKLTAAMADHSNHIRNMLVQAEDARLMGDLKTMKKRYIELYDLNRDLINEYKIRSNNHNALLACLKAVNQAIQRAGRLRVGKPKNQVITACRDAIKNNNINALFKIMRAGTASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CI48</accession>
    <name>A0A3B4CI48_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CI48"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011677"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036215.2">
        <property type="protein sequence ID" value="ENSPNAP00000011677.2"/>
        <property type="gene ID" value="ENSPNAG00000017350.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286997"/>
    <dbReference type="OMA" id="CCFHCVS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF519">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043433663" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="21"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="504"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="619"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="730"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="504"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011677.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="773" mass="86391" checksum="DBF05B2FA5A4D283" modified="2024-11-27" version="2">MGKLSVMHLCFLLCNQTCSACELLGKFEMHDMFKEGDIMIGGIFPIFNKQENVLTSFEIQPPKADCKGFDLRAFRWTRTMIFAIDEINSDGHLLPNISLGYKILDSCASPSNVLRCRPPPIAALVSYFSTCACLSDKSKYPTFFRTIPSDYHQAKALAFLVKRYGWTWIGALQSDNDYGRNGISAFTKEVEQHGVCIAFVGTILRTYPQSKILEVVEMIKQSTVKVILAFVPEGDLYPLMKEVVNQNIMGIQWIASEAWITADRPSTTEMFQSFGGTIGFVVRKMPMPKLGPVLKDIRTEKIDYTNKFFDVTQLRVAYNVYQAVYAIAHALHQIMFCEMPESNFSRLCLNTSQITPKLQLKKVNFVDAYGEKVFFDDKGDPPASYEVINWQLREGQVQHIAVGHFSTSTNGRYELEINEDLIVWNTGNLVTPKAVCSEICPHGTRKAQIKGLPVCCFDCIPCADGTISNTTADCTTCPEEFWSNDRKDSCIMKVTEFLSYAETMGIVLIAVSLFGACLTFSTMVVFICFRETPIVKANNSELSLLLLLSLIFCFLCPLTFIGEPTVWSCMLRHTAFGITFALCISCVLGKTIVVVTAFRATLPGNSVAGKFGPAQQRGIVYSCTAAQIVICVLWLKITPPFPDKVFEQRNKKIILECNTGSDAAFYSVLGYIGLLATICFALAFLARKLPDNFNEAKFITFSMLIFCAVWITFIPAYVSSPGKYTVAVEIFAILSSAFGLLLCIFMPKCYIILFKPETNTKKHVMGKNLKHTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CL32</accession>
    <name>A0A3B4CL32_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-associating domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574364.1">
        <property type="nucleotide sequence ID" value="XM_017718875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CL32"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012063"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019202.2">
        <property type="protein sequence ID" value="ENSPNAP00000012063.1"/>
        <property type="gene ID" value="ENSPNAG00000017770.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440141"/>
    <dbReference type="CTD" id="100006341"/>
    <dbReference type="GeneTree" id="ENSGT00940000158546"/>
    <dbReference type="OMA" id="MDGTQDG"/>
    <dbReference type="OrthoDB" id="9976881at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033614">
        <property type="entry name" value="RASSF1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011524">
        <property type="entry name" value="SARAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22738:SF14">
        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22738">
        <property type="entry name" value="RASSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16517">
        <property type="entry name" value="Nore1-SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50951">
        <property type="entry name" value="SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="3">
        <location>
            <begin position="161"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="SARAH" evidence="4">
        <location>
            <begin position="257"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="35863" checksum="8ACDB8B85502D0A5" modified="2018-12-05" version="1">MMASVNLDQVQIGENKYISKKALLSNLKTYNLYYRGEALQLRHREEEGELIIEGLLNIFWGLRRPIRLQMQDGKEPIKPAPFLSGRYANTAESEKQRKKLVPPTIEVTEPADQSEQGEEEESAHLQRTRSDVSGRRLGGRTALGNQRRIRRHRCSFNGHYYNHKTAVFTPAFGSVTNVRINSSMTTAQVLRVLLNKFKIENSPEDFSLFLLHTSGERVQLKRGDHPLSIRVLQGPCEQVSKIFLMEVDQVEEVTYDVAQYIKLEIPVLQSFIDKLMEEELREMEKLKQRYSDLRSMIQMHMKRLADKAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMB2</accession>
    <name>A0A3B4CMB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plakophilin 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PKP2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571883.1">
        <property type="nucleotide sequence ID" value="XM_017716394.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMB2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011694"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018847.2">
        <property type="protein sequence ID" value="ENSPNAP00000011694.1"/>
        <property type="gene ID" value="ENSPNAG00000017362.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438525"/>
    <dbReference type="CTD" id="5318"/>
    <dbReference type="GeneTree" id="ENSGT00940000158677"/>
    <dbReference type="OMA" id="VCHILNN"/>
    <dbReference type="OrthoDB" id="3245100at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002934">
        <property type="term" value="P:desmosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045110">
        <property type="term" value="P:intermediate filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF25">
        <property type="entry name" value="PLAKOPHILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="341"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="786" mass="87199" checksum="D19BBA862AAA407A" modified="2018-12-05" version="1">MAAEESLYMRTVLSLQGSVKPDDSSLALPSEDRLARSDSHTVSHRRCRVEQQVQQLTLSRRGKGSALKGSLSIGHLATSTPDRNGQVKASSFASESIKPVRRVDVSHWPSLELPQFNAGYGTLRYGSSGKSFCWRSSAFTLPAMRSVFSADHLQATVPSQHYAHSDTLKSTRPFVSGRSATLQLPSKPIYEDDPLDDVFLSEGPHITPFRSEDQMFLERQQVQRSFSQPREELHGSQDLSWLLREGWELQETASRRPSQTASQLNAEVYANGVAVAEESEAKQQARVNTVKRGKRGAEMTMRTAVSDLSQENTERQLTAAKFIQNQCYSSADAKKTLLHRRGIPKLLKLLDSEDEQLQQAAAGALRNAVFESTENKMEVKDCDGVAVILRLLSVNRNIEVRRQLTGLLWNLSSHDMLKEQLCRDAVKPLTDTVLVPCSGISEGEHPKLELLADPEVFLNATGCLRNISSAGPNGRKTMRDCNGLIDALVYYVRGTIADYKPDDKALENCVCILHNLTYQFESELPEALRPVLQESRQSLTLETHKPGCFSIRSAKIAETDNHEESDCPLLEEKGNPRGAEWLWSAITIRMYLSLVAISNRQLTQEASIGAMQNLTASTGGVSQAVAHIIVLRENGLQQVKKMLQEGEEEVRRVSLSLLKNLSRYKELYSDIIKQVLPELVAILPTTYTNTGQLVMATSICQILNNLSQASVQNASAALNQGVLPKIIKISTRDHRNGPTRAGQAAGVLLQTLWKHSELHGSYRKAGYRKTDFINSRTVRAVSSARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMI9</accession>
    <name>A0A3B4CMI9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MGAT4B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMI9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013252"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020767.2">
        <property type="protein sequence ID" value="ENSPNAP00000013252.2"/>
        <property type="gene ID" value="ENSPNAG00000003582.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156526"/>
    <dbReference type="OMA" id="FLMFHND"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056576">
        <property type="entry name" value="MGAT4_A/B/C_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23524">
        <property type="entry name" value="MGAT4A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGAT4 conserved region" evidence="4">
        <location>
            <begin position="112"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 A/B/C C-terminal" evidence="5">
        <location>
            <begin position="402"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013252.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="62992" checksum="8E1DE93394404EA5" modified="2024-11-27" version="2">MRLRNGTFLTVLVFGLCGLFSLSWYTAFSNPKGNVVDIYQREFLALRDRLHSAEQENLKRSKELNLVLDEIKRAIAEKQALRDINRTWSSLSGERERQLKLWNVTSSKNVLQLPSIFHHLPHLLAKETSLQPAVHIGQGRTGVSVVMGVPSVKREVHSYLTDTLSSLMSELSQAEKEDCVIVVFIAETDQQYANSVADNLKRLFPGEIQSGLLEIISPSVHFYPDFSNLKESFGDPKERVRWRTKQNLDYCFLMMYAQSKGTYYVQLEDDIVARPNYFTTMKNFALQQPSEEWMILEFSQLGFIGKMFKALDLSLIVEFMLMFYKDKPIDWLLDHIMWVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQTLHKGHANPLAEVTTSLKTYQHFTLEKAYLGEDFFWAFTPVAGDFIRIRFFTPVRIERYFFRSGNIEHPGDKLFNTTVEVLPFDVSVCVSPSALTHSTHTLQYKWPRGNLGLLQGFQNGIAEGEVDPSFGPLEAMRLSVTTDSPVWVILSEVMVNLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CST2</accession>
    <name>A0A3B4CST2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">KCNG1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017549662.1">
        <property type="nucleotide sequence ID" value="XM_017694173.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CST2"/>
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        <property type="protein sequence ID" value="ENSPNAP00000015157.1"/>
        <property type="gene ID" value="ENSPNAG00000021172.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159686"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:delayed rectifier potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein homooligomerization"/>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01492">
        <property type="entry name" value="KV6CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
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        <property type="entry name" value="POZ domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="289"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="301"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="378"/>
            <end position="399"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="411"/>
            <end position="428"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="440"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="16">
        <location>
            <begin position="66"/>
            <end position="165"/>
        </location>
    </feature>
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        <location>
            <begin position="228"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022538"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF00520"/>
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        <source>
            <dbReference type="Pfam" id="PF02214"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015157.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="58373" checksum="55DB5BEE69D6D9B3" modified="2018-12-05" version="1">MTLLAGDGSDYDYSALSCASDTSLNPPPVQEREALKGVYYKRAQCLFSSDTADAAACPGDGQRLHVIINVGGLRYQLPWTTLEDFPLSRLGQLRLCSSFDEIMRVCDDYDVTRNEFFFDRSPCAFRTILTFLRAGKLRSLREMCALSFREELLYWGVPEESLEWCCRRRLLQRAEECDELERAAEEEEEEDEDDEESEGGLAGPSRLGRCMGKLRDMVEKPHSGLPGKIFACLSVLFVTITAINLSISTMPAMREEEEDGKCSQMCYNIFIVETVCVAWFSLEFTLRFIQDRSKLAFLRRPLNLIDVVAILPYYITLVVDSTSAGEKRLGSGSTYLDKVGLVLRVLRALRILYVMRLARHSLGLQTLGLTARRCTREFGLLLLFLCVAIALFSPLLYLIENEVAATQEFSSIPATYWWAVITMTTVGYGDMVPRSIPGQVVALSSILSGILLMAFPVTSIFHTFSRSYVELKQEQQRLLQRRTHFLLRSRMAGLGSNMSLESDVLFPSASSDPRDTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTD8</accession>
    <name>A0A3B4CTD8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017556884.1">
        <property type="nucleotide sequence ID" value="XM_017701395.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTD8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013794"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021493.2">
        <property type="protein sequence ID" value="ENSPNAP00000013794.1"/>
        <property type="gene ID" value="ENSPNAG00000019675.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429561"/>
    <dbReference type="GeneTree" id="ENSGT00910000144271"/>
    <dbReference type="OMA" id="HYTNWYV"/>
    <dbReference type="OrthoDB" id="5597713at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF87">
        <property type="entry name" value="ARGININE ESTERASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017275546" description="Peptidase S1 domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="26"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="251" mass="27826" checksum="035B21DB68AB4A01" modified="2018-12-05" version="1">MTLISLFLLAALLPYLSHSASVEVGIVNGTEVKPHSRPYMVSIQKKRKHVCGGFLVSEKFVMTAAHCRKKIKQLTVVLGAHDLSNKKDGSIRMKVKKYYRHPDYNIDTHDNDIMLLKLRGRAKKSKTVNWISIPKRNEDIKTNTVCSVAGWGRTGSFKSGSNRLLETKITIVAKKQCKKLWENYLTRRMVCAVHPGGSCKGDSGGPLVCGDIAVGIVSFGQKNMCDAPTKPKIFAKISAFLPWIKSIIGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVM4</accession>
    <name>A0A3B4CVM4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ALMS motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVM4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024563.2">
        <property type="protein sequence ID" value="ENSPNAP00000016152.2"/>
        <property type="gene ID" value="ENSPNAG00000006246.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153123"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046599">
        <property type="term" value="P:regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029299">
        <property type="entry name" value="ALMS_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553:SF24">
        <property type="entry name" value="(E2-INDEPENDENT) E3 UBIQUITIN-CONJUGATING ENZYME FATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553">
        <property type="entry name" value="ALMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15309">
        <property type="entry name" value="ALMS_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ALMS motif" evidence="5">
        <location>
            <begin position="119"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016152.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="253" mass="28472" checksum="A0D10DAE463EEEE1" modified="2024-11-27" version="2">MVTQQSSLTPVFFSLLSEPAAINKRLDSLAVIRNSCAVVSVKCHGLEEKTQNSSHSEDAKGFSPSKRLLVNSGGSGRGSYDGRVPCGHAETVTKVQEGLVFPHMLNCTCGSNSSNQPHILSLKEALELFRPDFISRSQRRIRHLEQRARERRSLQGADLMKEIESTNKARSCTKPHPLSDNLFKPKDRVISGKEMQLRSRRIYNKLPEVTKKKEEEKRRLVLQTNRLRAEVFKKLYIKIWVQAPMSGTNGDSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVT3</accession>
    <name>A0A3B4CVT3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Cyclin-dependent kinase inhibitor 3</fullName>
            <ecNumber evidence="4 11">3.1.3.16</ecNumber>
            <ecNumber evidence="3 11">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CDKN3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with cyclin-dependent kinases such as CDK1, CDK2 and CDK3. Does not interact with CDK4. Interacts (via C-terminus) with phosphorylated CDK2 (via C-terminal helix). Interacts with MS4A3 (via C-terminus); the interaction enhances CDKN3 enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4 11"/>
    <dbReference type="EC" id="3.1.3.48" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_017537913.1">
        <property type="nucleotide sequence ID" value="XM_017682424.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537914.1">
        <property type="nucleotide sequence ID" value="XM_017682425.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVT3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015448"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023711.2">
        <property type="protein sequence ID" value="ENSPNAP00000015448.1"/>
        <property type="gene ID" value="ENSPNAG00000021480.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411052"/>
    <dbReference type="CTD" id="1033"/>
    <dbReference type="GeneTree" id="ENSGT00390000004717"/>
    <dbReference type="OMA" id="CRYKDIR"/>
    <dbReference type="OrthoDB" id="19045at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14505">
        <property type="entry name" value="CDKN3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000046">
        <property type="entry name" value="Cyclin-dependent kinase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008425">
        <property type="entry name" value="CDK_inhib_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022778">
        <property type="entry name" value="CDKN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05706">
        <property type="entry name" value="CDKN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037322">
        <property type="entry name" value="CDKN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="116"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="12">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037322-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="209" mass="23560" checksum="7165582065A02E49" modified="2018-12-05" version="1">MKISEFDSSDEEEEAGDEEHTPLQISWLSLSVVECSQFLGICSLPGCRYKDIRRNLEKDVGDLCAEGVEDVFVFCTRAELVRYRVPCLLERYTERGLVVHHLPFPDGGAPEMPQCCHILEELQLSLQNQRRTVIHCYGGLGRSGLIAACLLLHLSVSMTPSKAIDILRELRGSGAIQTVKQYNFLHEFREKLAAYQETKGTVSERSVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYJ6</accession>
    <name>A0A3B4CYJ6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">COPS7B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYJ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015619"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023868.2">
        <property type="protein sequence ID" value="ENSPNAP00000015619.1"/>
        <property type="gene ID" value="ENSPNAG00000021663.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157155"/>
    <dbReference type="OrthoDB" id="10265275at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="240" mass="26662" checksum="2B2338EACB6DF72E" modified="2018-12-05" version="1">MASEQKCGHGSPLQQFVLLAKNLRGAALVSLINRMLETPGVYTFGEFLDLPQVKELSKGPDDGYSRLLELFAYGVYHDYQANESTLPPLSDAQRNKLRHLTIATLAANTQCIPYSVLQTALEVASVRQLEDVLIEAVYSDVINGKLDQCKQQLEVDACIGRDVCSKDTGELAQTLKSWCTRCDSMCCAIESQVHKVRQSRDCLLLIQQQVHTEEGLPGETEQRVSYKSTSKTKNISSPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0H3</accession>
    <name>A0A3B4D0H3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0H3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017817"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026743.2">
        <property type="protein sequence ID" value="ENSPNAP00000017817.1"/>
        <property type="gene ID" value="ENSPNAG00000024107.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158347"/>
    <dbReference type="OMA" id="NQTETRF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02122">
        <property type="entry name" value="PA_GRAIL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16802">
        <property type="entry name" value="RING-H2_RNF128-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051073">
        <property type="entry name" value="ZNRF3_Arkadia_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16200">
        <property type="entry name" value="RING ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="287"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="434" mass="49229" checksum="80B134E8B81C02C5" modified="2018-12-05" version="1">MEKDERKSQRFSWIWLALLISQFPAHSRAFMVFWSAYVELRYFSQVTNQSTTSVCECGVYGLDSPLYAESGYVALPNSDSVACSPNITFAVNHKPWIALIKRGNCSHADKIRAAEQEGASAVVIYNIDGTGNSTNPMSHPGTGSTVAIMIGNHMGRQITSLVESGTEVYMSLLVGNPYKTWDNPLWVYVMSFTFFGITAIILGYFVFVVIKRLYRNRQLRIQQRELKKVAERAIAKLQVRTLRRTDPVQRSNYIVTKLLIFKNNNQCNLYNYNCISLQEVEAEENNCAVCIDPFKRGDIVTTLPCSHLFHKTCIEPWLLEHHTCPMCKYDILKGEVGVEPTQESSMPPADVRFYPSSVSGSLTETPDVVRSLEQQAEARPGLGLQTAECAIQLSNHIYEEPYAMREGHIYENPTFEEEQQITEYQDSNQQTHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0U2</accession>
    <name>A0A3B4D0U2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 51</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCDC51</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017580509.1">
        <property type="nucleotide sequence ID" value="XM_017725020.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0U2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017168"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025853.2">
        <property type="protein sequence ID" value="ENSPNAP00000017168.1"/>
        <property type="gene ID" value="ENSPNAG00000023375.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444052"/>
    <dbReference type="CTD" id="79714"/>
    <dbReference type="GeneTree" id="ENSGT00940000164287"/>
    <dbReference type="OMA" id="STATTWW"/>
    <dbReference type="OrthoDB" id="6243211at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037660">
        <property type="entry name" value="CCDC51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28624">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28624:SF1">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="48162" checksum="B298D2AA9AC16AD0" modified="2018-12-05" version="1">MRYRGSCILPRSYGTCFYLLQVPVRAGRVRYNVSINRTYCSSKESPQNNVVSTQEKSLATLKNVAELGKQWGRNSVRTMTTTVNYWWERYEEFVGLNEVREAQSKVTEAEKAFMIARGMVREAHSSLEALQIRLKEVRDRLDRVSREEAHYLELATLEHKLLQEERRLKTAYENAEDGEREKFALFSAGVRESHEKERSRAERTKNWSIIGSVLGAIIGVMGSTYLNRVRLQELKGLLLEAQKGPASLQEAIKIQASLHKNQQEELRGLIDTLRAAVKNGPDELQTVKRVSVPVPLPVPTAVPPSTPSASEEAVKEILLRSEKAQALMEDLRPQLEQLERSMGKVATEIQAVKKSVLTRPAEKPVIQTKDTQLLVCDTESIIQGLDQTEKRLEEQINKTTLYSTVLTYTAFALTMPVLYIIFKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D374</accession>
    <name>A0A3B4D374_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IRF tryptophan pentad repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017537547.1">
        <property type="nucleotide sequence ID" value="XM_017682058.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D374"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017958"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026918.2">
        <property type="protein sequence ID" value="ENSPNAP00000017958.1"/>
        <property type="gene ID" value="ENSPNAG00000007121.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410787"/>
    <dbReference type="CTD" id="569263"/>
    <dbReference type="GeneTree" id="ENSGT00940000166222"/>
    <dbReference type="OMA" id="GYQMHPS"/>
    <dbReference type="OrthoDB" id="8537190at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000041">
        <property type="entry name" value="Interferon regulatory factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019817">
        <property type="entry name" value="Interferon_reg_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001346">
        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019471">
        <property type="entry name" value="Interferon_reg_factor-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
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        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="INTERFERON REGULATORY FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
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        <property type="entry name" value="IRF"/>
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        <property type="entry name" value="SMAD/FHA domain"/>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IRF_1"/>
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    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="7">
        <location>
            <begin position="15"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PROSITE" id="PS51507"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017958.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="433" mass="49050" checksum="858BCE0A0544BB27" modified="2018-12-05" version="1">MCSDGERATAGSSGNGKLRQWLIEQVDTGKYPGLVWENDEKSVFRIPWKHAGKQDYNRDEDAALFKAWAVFKGKYREGIDKPDPPTWKTRLRCALNKSNDFEELLERSQLDISDPYKVYRIVPEGAKKGPRSLEDTPPNTSPPSYPLHPSYAPIQSQVCNFISPVERSWRDYLSDKPPISDIPYNQNPFSPRWDTGYQFSGSFYSCSASEPLPSAFTLDSSMKSAEAMALSDYRLQVAVFFRDALVREMTVSSPEGCVLGVSRDGLPYGASEGPELVELPQVERGTLEHGVLLWMAPDGLYARRRCPCRVYWEGGHTAASNKPGKLEREQTCKLLDTQLFITELQSYALHGRPPPFSQVLLFFEDESAEPQRPRRTITVQVEPLFARQLLYLTHPSNMNYIRSHNLSPLPPEHAIASSQDFHRVITHHHSNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4X5</accession>
    <name>A0A3B4D4X5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">GRIK4</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15 20">Postsynaptic cell membrane</location>
            <topology evidence="15 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Presynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037402661.1">
        <property type="nucleotide sequence ID" value="XM_037546764.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037402662.1">
        <property type="nucleotide sequence ID" value="XM_037546765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D4X5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018513"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038996.2">
        <property type="protein sequence ID" value="ENSPNAP00000018513.1"/>
        <property type="gene ID" value="ENSPNAG00000024965.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439963"/>
    <dbReference type="GeneTree" id="ENSGT00940000159111"/>
    <dbReference type="OMA" id="LVWLMKI"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13724">
        <property type="entry name" value="PBP2_iGluR_kainate_KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000060">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="glutamate receptor ionotropic, kainate 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutamate receptor, ionotropic, kainate 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000010">
        <property type="entry name" value="Putative glutamate receptor ionotropic kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="Glu/Gly-bd"/>
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        <property type="entry name" value="Iono_Glu_rcpt_met"/>
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        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
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        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 20" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6 20" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 20" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027153457" description="Glutamate receptor" evidence="20">
        <location>
            <begin position="21"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="547"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="586"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="805"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="22">
        <location>
            <begin position="417"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="23">
        <location>
            <begin position="427"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="936"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="949"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="675"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="723"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="18">
        <location>
            <position position="653"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="18">
        <location>
            <position position="769"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="735"/>
            <end position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="966" mass="108152" checksum="CFF2F27194A420E4" modified="2018-12-05" version="1">MLAAWAPLLFLAMWVRSTASGPLSFRIAAILDDPMECSRGERLAITLAKESINRSSNRSTTGKLEVDIFELLRDSEYETGETMCQIMSKGVVAVLGPSASPASNSIISNICGEKEVPYVKVAPEDILKVQFPRFTTLDLRPTNTDVSLAVAGLLTFFNSTTSCLICAQANCLLNLEVLLRQFLISKETLSVRMLDDSQDPTPLLKEIRDDKTATIIVDANATMSHIILERASELGMLSVYYTYIFTSLEFSLLRLDDVADQRVNIVGFSVFNKTHPFFQDFLLSLNRSWQENCDHAPFAGTPLSSALLFDAVHTVVAAVQELNRSQNVGATQLSCKSSKIWEHGTSLMNYLRMVELEGLTGHIEFNSRGQRSNYALRIMQNSRDGLRQIGQWHSEQGLSMERKLPSLNVTDTLFNTTLIITTILENPYVMLKSNYQDLQGNDRYEGFCVDMLKELADILKFKYRIKLVSDGVYGVSGANGTWTGMVGELIARKADLAVAGLTITAEREKVIDFSKPFMTLGISIMYRVHLGRRPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYNPHPCLKGRCSLLINQYSLGNSFWFPVGGFMQQGSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTMHGGSTMTFFQNSRYQTYQRMWNFMHSKQPSVFVKSTEEGIARVLNSNYAYLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPLGSVYRDEFDLAILKLQEENRLEILKRKWWDGGKCPKEEDHRAKGLGMENIGGIFVVLVCGLLVAIFMAVLEFVWMLRQTPGTELSVCEEMLRELQGIVLCRDSLQLRRRRGGSSALRPRPLALEERRQRATAASLSNGKLCTGSGLPEPLPHRLAQEAALAARGCSHIRICPECRRFQGLRVRPAGPPGASPTHSEDSMEWEKTTNSSEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5K0</accession>
    <name>A0A3B4D5K0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Integrin alpha 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5K0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018391"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027600.2">
        <property type="protein sequence ID" value="ENSPNAP00000018391.2"/>
        <property type="gene ID" value="ENSPNAG00000024765.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158443"/>
    <dbReference type="OMA" id="AGHHNTI"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001568">
        <property type="term" value="P:blood vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060956">
        <property type="term" value="P:endocardial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043114">
        <property type="term" value="P:regulation of vascular permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF78">
        <property type="entry name" value="INTEGRIN ALPHA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1032"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="98"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="345"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="407"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="468"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="15">
        <location>
            <begin position="515"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="16">
        <location>
            <begin position="675"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="17">
        <location>
            <begin position="829"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018391.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1084" mass="120012" checksum="304EB4F373CABBD2" modified="2024-11-27" version="2">MLSSVWGPTQREGSPVRADAPQSATGARDAYTHALALTFSCHELGVRVRRKVRALRLQSRAALEFVPFTTSGAMRRALACVCAWCCVLILSHGYNLDTRSPESFRGQNGSMFGYSVLLHRHQQNPWLLVGAPTDDSSFNASIKKPGAVYKCNISNKKCEQLRTGVEHCGKTCWPESDNQWLGVSLSRRPSNGDILACGHRWKNVFFSRKDNQNKLPHGVCFKFNSDFSRTNNYIPCYRDHQRKFGENYGSCQAGISNFLTEDLMIMGAPGSWYWTGSVLVHNMSSNMLAAYVDEDNTVQFGSYLGYSVGAGHFLHPQTTEIIGGAPQNGRIGKAYIFEVEGNKLRIVNETSGAQLGSYYGASICAVDLNADGLSDLLVGAPMYSTVREEGRVYVYINQGRANMKEAEFKLVGSESYAARFGETITDLGDIDNDGYPDVAIGAPQEDDLRGAIYIYNGRKSGIASRFSQRITGSMLGGSFSMFGQSVSGGVDVDGNGYPDVAVGAFLSDSAVVLRTRPVVMVEASMMLPVSVNRSVASCAENGLPAVCVNVTVCFKVQGKRIPGQIELKYNLTADVQHKETFPPRFYFHGNGTSNVTAGRVKAKHGELTCSQHQAFMRKDVRDIFTSIHFKVQYELGEHSVVRGGSKSFTSLKPILQQREGHTNLVSNQTQFARYCVWENCSTNLQISARLVLPQSYNNVPYFALGNGKTIMLNATLVNAGDDAFLPKLHVRFPSSLHFIRVLDAEEKYVSCEIAEDNKTAIGLDCSVGNLYIPSLSKLNISFLMDVNQNSSAGDIGISINATRENYENTDLLHDNLASLTLPLRYGVGLNVHGFVSPTTFIFGNPELEPVDCYSEKFNYTFKVMNVGPSKALDTRVKIQIPQVLSPYPDRLIRITDLQTTVGQCHLKNSSLWLENDCDVKKASFIQELVFFLSKSSKRIMVKVSDKSCMNLECRFGNLDIGKEVTINLEVELNPAVLQISPGRHAIMQMYSVIEVTSPVRDANTILIKDDNVALVTLEAYYSQKPHPLVGNAIIVFSLLLGLIILAMLVVCLWKAGFFKRRIKEEQDKFRRQSWDYVPKYESVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5L5</accession>
    <name>A0A3B4D5L5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein arginine N-methyltransferase 3</fullName>
            <ecNumber evidence="3">2.1.1.319</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">PRMT3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017574086.1">
        <property type="nucleotide sequence ID" value="XM_017718597.1"/>
    </dbReference>
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        <location>
            <begin position="72"/>
            <end position="117"/>
        </location>
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        <location>
            <begin position="250"/>
            <end position="322"/>
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            <begin position="355"/>
            <end position="511"/>
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            <begin position="1"/>
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        </source>
    </evidence>
    <sequence length="522" mass="58308" checksum="D507CF2F3C13953D" modified="2018-12-05" version="1">MAEPTDTRHDEVPDLSLSEGEEEEDDEEQWQWMEEEADGVSVSCLFCQRSLNSVPETFQHCQADHGVSILQLVKAHRLDDYGYIKMINYIRTVKCSGETLAVFDGAVPWDSDEFMKPALPDDPLLQIDLEELVGAELVVDAAASGQDEALLQRARQAEERAARSEEALARAMEDLQNLKVLAQGLVLNAGRPGPSCSGAIAELREDEDEAYFSSYGHYGIHEEMLKDKVRTESYRDFICGNPDVFKDKVVLDVGCGTGILSMFAARAGARKVIAVDQSEIIYQAMDIVRSNQLENTITLIKGRIEDVNLPVEKVDIIISEWMGYFLLFESMLDSVIFARDMYLAEGGSVYPNSCNISLAAVGDTEKHCDRIAFWDDVYGFKMECMKKAVVPEAVVEVLKPETVISEPAVIQTLDCNAVTTSELEFTADFTLKMTASTHCTAVVGYFDIFFDRGSANKVMFSTSPHCTKTHWKQTVFLLENPIPVQSGDELPGRITVRKNRKDPRALLVTLNLADWRQTYSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D609</accession>
    <name>A0A3B4D609_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">trypsin</fullName>
            <ecNumber evidence="10">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="10"/>
    <dbReference type="RefSeq" id="XP_017557493.1">
        <property type="nucleotide sequence ID" value="XM_017702004.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D609"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018359"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038939.2">
        <property type="protein sequence ID" value="ENSPNAP00000018359.1"/>
        <property type="gene ID" value="ENSPNAG00000024733.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429926"/>
    <dbReference type="GeneTree" id="ENSGT01050000244883"/>
    <dbReference type="OMA" id="IARSKCE"/>
    <dbReference type="OrthoDB" id="10012881at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000008">
        <property type="entry name" value="Cationic trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000122">
        <property type="entry name" value="Chymotrypsin-like elastase family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF7">
        <property type="entry name" value="TRYPSIN-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017177884" description="trypsin" evidence="12">
        <location>
            <begin position="19"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="23"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="249" mass="27404" checksum="7107C70F84761CC8" modified="2018-12-05" version="1">MRSAVAVFLLVAVAFSRADDDKIIGGYECPAHSQPWQVFLTYDDGERWCGASLINERWAVSAAHCYIPPPRLAVHLGEHNLFNEEGTEQRIWAEEVIPHPDYNDVTYDNDFMLIKLSEPAVFNEYVQPIPLATTCAQEGEQCLVSGWGNQINTGVNYATVLQCLDVPVIARSKCEASYPNKITKNMFCAGFLEGGKDSCQGDSGGPVVCNGKLQGVVSWGYGCAYPGYPGVYAEVCQYVDWVRDVIASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8S9</accession>
    <name>A0A3B4D8S9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histamine H2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Gastric receptor I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">HRH2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8S9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019910"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030044.2">
        <property type="protein sequence ID" value="ENSPNAP00000019910.2"/>
        <property type="gene ID" value="ENSPNAG00000026629.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158761"/>
    <dbReference type="OMA" id="CIFRMAR"/>
    <dbReference type="OrthoDB" id="5951059at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000121">
        <property type="entry name" value="Histamine H2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF278">
        <property type="entry name" value="HISTAMINE H2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="21"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019910.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="360" mass="40210" checksum="5CEACC86CF3E7C2E" modified="2024-11-27" version="2">MMSTAFRWVVLVVFIVLTIGGNVLVILAVGTSRRLRRISNCFVVLLAITDLLLGLLVLPLSATLELGSGRWTLGGTFCNIYLSVDVLLCTTSIFSILAISIDRYLAISGPLSYSKRVTPLRVAAAIGAIWAVSMTVAFVSIHLGWNTVDFSVQNADWEMGDEGEEGRTCRYEWNNNYVLLDAFGTFFLPLLVMCGLYHRIFLVARAQARRIRAATPSFLRSASLVATAREHKATVTLAAVLGAFIICWLPYFIYFICMGLRREMDPPHLTHSVVLWLGYFNSALNPILYPALNRDFRQAYGQLLRCSTAQKWTPIPIRTLQQERSVSNGLQSEPHWNKQCGETRLNLREANDDASLEHGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D938</accession>
    <name>A0A3B4D938_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">High affinity choline transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Hemicholinium-3-sensitive choline transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 5 member 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SLC5A7</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">High-affinity Na(+)-coupled choline transmembrane symporter. Functions as an electrogenic, voltage-dependent transporter with variable charge/choline stoichiometry. Choline uptake and choline-induced current is also Cl(-)-dependent where Cl(-) is likely a regulatory ion rather than cotransported ion. Plays a critical role in acetylcholine (ACh) synthesis by taking up the substrate choline from the synaptic cleft into the presynaptic nerve terminals after neurotransmitter release. SLC5A7/CHT1-mediated choline high-affinity transport in cholinergic neurons is the rate-limiting step for production of ACh, thereby facilitating communication by subsequent action potentials. Localized predominantly in presynaptic terminal intracellular organelles, and translocated to the plasma membrane in active form in response to neuronal activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>choline(out) + n Na(+)(out) = choline(in) + n Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:76443"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homooligomerizes at cell surface. Interacts with SEC14L1; may regulate SLC5A7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Presynaptic cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 19">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D938"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019626"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030199.2">
        <property type="protein sequence ID" value="ENSPNAP00000019626.1"/>
        <property type="gene ID" value="ENSPNAG00000026299.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00690000101915"/>
    <dbReference type="OMA" id="QSESIMV"/>
    <dbReference type="OrthoDB" id="546820at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005307">
        <property type="term" value="F:choline:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008292">
        <property type="term" value="P:acetylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11474">
        <property type="entry name" value="SLC5sbd_CHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000008">
        <property type="entry name" value="High affinity choline transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052244">
        <property type="entry name" value="Choline_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45897:SF2">
        <property type="entry name" value="HIGH AFFINITY CHOLINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45897">
        <property type="entry name" value="HIGH-AFFINITY CHOLINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="271"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="589" mass="63915" checksum="51D4DC547FF7E39D" modified="2018-12-05" version="1">MALHLEGLLAIVIFYVLILFVGIWAAWKNKNSGVEGGSDQSESIMVGGRDIGLFVGGFTMTATWVGGGYINGTAEYVYLPSHGLAWAQAPFGYALSLVVGGLFFAKPMRSRGYVTMLDPFQQLYGKRMGGLLFIPALMGEIFWSAAILSALGATLSVIVDININISVMISALIAIFYTLVGGLYSVAYTDVVQLFCIFVGLWISVPFALANPAVSDIGVTAVKRVFQTPWLGTIQPSDGWMWADNFCLLMLGGIPWQVYFQRVLSASSATYAQVLSFLAAFGCLVMAIPSVLIGAIGASTDWNQTSYGPVPPMEKDESDMILPIVLQHLCPPFVSFFGLGAVSAAVMSSADSSILSASSMFARNIYQLAFRQSASDREIVWVMRVTIFVFGALATAMALLTGTVYGLWYLSSDLVYVIIFPQLISVLFIKGTNTYGSVAGYVFGLLLRIGGGEPYLRLPPFIYYPGWTQEEKVHHLTKEVEHIVMQRFPFKTVAMLASLLGNAVFSYAAKYLFESGTLSPKYDFLDAVVSKHSKEIMDKTTLVGSDNIILSEMAPVKPRIGSSLAGTFMNAEALSDEGETSPDLNNEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBF4</accession>
    <name>A0A3B4DBF4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017555686.1">
        <property type="nucleotide sequence ID" value="XM_017700197.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBF4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020274"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030713.2">
        <property type="protein sequence ID" value="ENSPNAP00000020274.1"/>
        <property type="gene ID" value="ENSPNAG00000027063.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428852"/>
    <dbReference type="GeneTree" id="ENSGT00940000167241"/>
    <dbReference type="OMA" id="DPSYRVC"/>
    <dbReference type="OrthoDB" id="289416at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038800">
        <property type="entry name" value="CCDC17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33820">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33820:SF5">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 17-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="70141" checksum="C2E65FB5606706D4" modified="2018-12-05" version="1">MDSLVCSTCNMSFRSSHFLAKHKERFCIGDADHQTQESDFTQTMGERIRELRRKHQQERNNKGTQRSQQNLDTTTQHELRKRNSEPTADRTHPQIQDLFEIPKRQAPQSSDSKKWNKQRREINQQELIVQNRKVAQLEKMLLELKEQEKRNTTLLESLIDHLQHDPTEPTAVTSKGRDTLQSSNPHLLIPETREEVTQTYVPVYGGGVLSSEISTLRLSYLQNGGKDPHILAQLQDLLNEALNVEILPFPKTYHTERTKHGPKPKDNKKDFFRELISTEIKNQHLEEEIRRLQLRKRRSAMTRKTPDYQPSEQEMRSMKMDIDLLKNEIEINHLRKMIRSRKVEPTLTTFPPLEEPRLQTPPYTKYSMDVSEGLSPAPYDPMAGFVVFYDFLLGLCPSYRVCRLMVGLYSGEQCLGSPSVLPPVYCEPFILSTHPSRYNQGQKAVLASKQAVPEVQPAPSASLVMQVQASGGYDIYGQEVTHLAPRGWVKLDIFDHHNRIISGRWKIPVRILPAKPSMTTAEVNTVPQLDNADLYLRIVNARDAEIQSAVPISIYTAGIYRYFPVTTARHHFSRQDSHEPVQTPAVQSAQVYIPPCTQSLDPPSTGLFDNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGD3</accession>
    <name>A0A3B4DGD3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGD3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034765.2">
        <property type="protein sequence ID" value="ENSPNAP00000022565.2"/>
        <property type="gene ID" value="ENSPNAG00000004688.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022565.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="113" mass="12404" checksum="22C5AEEC6E246F95" modified="2024-11-27" version="2">SIFLGMTSNFSLQVLDELFGLSEEVQPLVQANGREGSAEAMTSLLRDQRDVSKEGDPMHAMERGAEPGHVWPRLLLDIMGHQNRFRGRTKKNMAQGCMGHELHPNGAGEVGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMD1</accession>
    <name>A0A3B4DMD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMD1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024094"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011009.2">
        <property type="protein sequence ID" value="ENSPNAP00000024094.2"/>
        <property type="gene ID" value="ENSPNAG00000037161.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286924"/>
    <dbReference type="OMA" id="PVQRILM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484:SF7">
        <property type="entry name" value="MYELIN-ASSOCIATED GLYCOPROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484">
        <property type="entry name" value="SI:CH211-171H4.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043904638" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="121"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024094.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="302" mass="34834" checksum="57FBDEEF2C5955FE" modified="2024-11-27" version="2">NMRTQGRLLFCWLLIFATVLSDVWKAEVVSELKTLVSSCVVLPCKFQYPGKQLPDSRIRGIWHQQAKHDQRIYHEDSTEIADSFKGRTRLLGHLGEKNCTLEIDEVKNHDNGPFCFRAEIPSIDKYSFVESCVALNMMCKYDCKELKLEQMKTLEDGVPATFKCSIRHTCPSHPPTITWSRSNSEATWSNRDILHGNWEVESLLIFIPTEKDDFTHLNCTVSFHGGLQSSVSSLIHVKRKSCSLSTIVAHITFTKMFYFLVHQSQRSRNIGTNMLQFQVRNYVHSYLLSNCKRLNLIISQHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPK2</accession>
    <name>A0A3B4DPK2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SCML4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568456.1">
        <property type="nucleotide sequence ID" value="XM_017712967.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393904.1">
        <property type="nucleotide sequence ID" value="XM_037538007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPK2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024869"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009023.2">
        <property type="protein sequence ID" value="ENSPNAP00000024869.2"/>
        <property type="gene ID" value="ENSPNAG00000009671.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436462"/>
    <dbReference type="CTD" id="256380"/>
    <dbReference type="GeneTree" id="ENSGT00940000157463"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09578">
        <property type="entry name" value="SAM_Scm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000018">
        <property type="entry name" value="Polycomb protein scmh1 isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.190:FF:000001">
        <property type="entry name" value="Polycomb protein scmh1 isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.190">
        <property type="entry name" value="SLED domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047531">
        <property type="entry name" value="SAM_Scm-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033763">
        <property type="entry name" value="SCML2_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021987">
        <property type="entry name" value="SLED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038348">
        <property type="entry name" value="SLED_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF85">
        <property type="entry name" value="SEX COMB ON MIDLEG-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17208">
        <property type="entry name" value="RBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12140">
        <property type="entry name" value="SLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="408"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="374"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024869.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="479" mass="52644" checksum="82939A2729046738" modified="2024-11-27" version="2">MRLRGRDLEDSAISWIPNVLGVQSFRLRLRLDRRDDRGRSLWQLVDLDEEAATNGQRLRDRTPPTCSQSLMSIPATSGQTGAGEMQSPAAVSPSFLPPPTGKVPGRKRGRPPLKRHPEYQNHYPESLPPIKVPKKRGRKPGFKLKSRLAMTPLAISPPSSTPEPDMSSIPQDAATVPHSATPEVLTVCIYVNKHVNTGPNLDRQKVLQLPDHLGPARPSVVLQQAVQGCIDSAYQQKAVFTLLTQGYGGEKISATFDGKQHLLSLPVVNSVGYVLRFLKKLCRSLLCENLFSDQPIAPSSSPSSSSSFHTDKHSEGQAKSTDSMVDDYHTEEMQSKRYSSDPTDSTFGALSSPYTAPKSSYGFRSGPTYSGGVCRQAASPSTFQEGNRSAYSPSPEAGESKPPSSKDPSRWSVEEVVWFIKDADPQALGPHVELFRKHEIDGDALLLLKSDMIMKYLGLKLGPALKLCYHIDKLKQNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DRM2</accession>
    <name>A0A3B4DRM2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium voltage-gated channel subfamily A member 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Voltage-gated potassium channel subunit Kv1.2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">KCNA3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.2/KCNA2 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DRM2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025614"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007182.2">
        <property type="protein sequence ID" value="ENSPNAP00000025614.1"/>
        <property type="gene ID" value="ENSPNAG00000010782.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160210"/>
    <dbReference type="OMA" id="IRECNCE"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000025">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000007">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF28">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="22">
        <location>
            <begin position="73"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="534" mass="59856" checksum="85ACAE9AB4216225" modified="2018-12-05" version="1">MDEHLHLLHSNNTINITNSINVNTHNHLLSLGHAPGLTHPPEAAMTVVSCDGAPEEPNSNAAERYEPEHECCERVVINISGLRFETQLKTLAQFPETLLGDPRKRTAYFDPLRNEYFFDRNRPSFDAILYYYQSGGRVRRPVNVPIDVFTEEIRFYELGEEAMEKFREDEGFIKEEERALPAGDFQRQVWLLFEYPESSGPARGIAIVSVLVILVSIVIFCLETLPEFRDDDDSDARDARISTTPLHLLHHYPDNVQANATGTLSLSLVTAPFSDPFFVVETLCIVWFSFELLVRFFACPSKATFSKNIMNIIDIVAIIPYFITLGTELAERQQGNGGGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADDPDSGFNSIPDAFWWAVVTMTTVGYGDMHPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQAQYLHIASCQPMPASPELRGARSSSSISRSEYVAMEEALQAKHNCVNKIFTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUE8</accession>
    <name>A0A3B4DUE8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LITAF</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUE8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027085"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003500.2">
        <property type="protein sequence ID" value="ENSPNAP00000027085.1"/>
        <property type="gene ID" value="ENSPNAG00000012487.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155366"/>
    <dbReference type="OMA" id="CWCVILA"/>
    <dbReference type="OrthoDB" id="4713066at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098560">
        <property type="term" value="C:cytoplasmic side of late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098574">
        <property type="term" value="C:cytoplasmic side of lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF45">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="10">
        <location>
            <begin position="96"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="180" mass="19797" checksum="785285865CFC857E" modified="2018-12-05" version="1">MDVPRCSSAHSMPPPPSYTEASQCPAYVTGPATPSTPPPSYGEAVGTSNEPVLSPFPVLTVPTVPTEITTVHQTQQVFVQQTIHSSPPQVFVVSHQETRRRQSRRRLLDRPTVINCPYCHHHITTTVEYKPGAAAWGMCCLFTLLGFICGCCLIPFCVTAFQDVHHSCPHCSKHIGIYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVG8</accession>
    <name>A0A3B4DVG8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SYNPR</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567219.1">
        <property type="nucleotide sequence ID" value="XM_017711730.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DVG8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028397"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014573.2">
        <property type="protein sequence ID" value="ENSPNAP00000028397.2"/>
        <property type="gene ID" value="ENSPNAG00000033303.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108435718"/>
    <dbReference type="CTD" id="558290"/>
    <dbReference type="GeneTree" id="ENSGT01030000234637"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF16">
        <property type="entry name" value="SYNAPTOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00220">
        <property type="entry name" value="SYNAPTOPHYSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="10">
        <location>
            <begin position="15"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="234"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028397.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="285" mass="31415" checksum="99E734F549497EE3" modified="2024-11-27" version="2">METANQIVSLGTFQVLKVPLGFIRLLEWLFAIFAFATCGGYSGQLRVSVDCVNKTDSNLSININFAYPFRLNQVYFDVPTCEGRSQERLFLVGDFSSSAEFFVTIAVFAFLYSLLATVVYIFFQNTYRENNRGPFIDFIVTVVFSFMWLVSSSAWAKGLSDVKVATDPDEVVLLMSACKVQTNKCGSAYGPIWSGLNTSVVFGYLNFVLWAGNIWFVFKETGWHKGGAARYPSSAPEKQAAPFNQPSYNQGTFDQSGGGFSGTGDLGQSEYSQVGTYTSSGPTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DW53</accession>
    <name>A0A3B4DW53_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BBSome complex member BBS1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Part of BBSome complex, that contains BBS1, BBS2, BBS4, BBS5, BBS7, BBS8/TTC8, BBS9 and BBIP10. Interacts with the C-terminus of RAB3IP. Interacts with CCDC28B and ALDOB. Interacts with PKD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017554235.2">
        <property type="nucleotide sequence ID" value="XM_017698746.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DW53"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027204"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003192.2">
        <property type="protein sequence ID" value="ENSPNAP00000027204.2"/>
        <property type="gene ID" value="ENSPNAG00000012672.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428043"/>
    <dbReference type="CTD" id="582"/>
    <dbReference type="GeneTree" id="ENSGT00390000005232"/>
    <dbReference type="OrthoDB" id="10259809at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005113">
        <property type="term" value="F:patched binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005119">
        <property type="term" value="F:smoothened binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001895">
        <property type="term" value="P:retina homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002248">
        <property type="entry name" value="Bardet-Biedl syndrome 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028784">
        <property type="entry name" value="BBS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032728">
        <property type="entry name" value="BBS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056419">
        <property type="entry name" value="GAE_BBS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20870">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20870:SF0">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14779">
        <property type="entry name" value="BBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23304">
        <property type="entry name" value="GAE_BBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0844">Vision</keyword>
    <feature type="domain" description="Bardet-Biedl syndrome 1 N-terminal" evidence="18">
        <location>
            <begin position="18"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Bardet-Biedl syndrome 1 protein GAE" evidence="19">
        <location>
            <begin position="481"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF23304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027204.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="588" mass="65126" checksum="F6971F37A4A72FDE" modified="2024-11-27" version="2">MASVHMEMKDSAVSSSKWLDAHYDPVANLYTFSSCIALADLHGDGENKLVVGDLGTGASNMKLKVYRGTGLLSENTLLDLPTGLVSFLMDQHEPCTPAIAVASGPFIYIYKNLRPYFKFTLPTLEVNPLEQDVWSQAREDMIDPMTLKEMLEGIRDKADIPLSVRSLRFLMLEPQDMEAYVNLHKQQPIRRQTVITCIGTLKKNMADDDAVSCLVIGTESQEVYILDPEAFTILAKMSLPSVPTLMDVTGQFDVEFRITIACRNGNIYILRRDSPRPKYCIELSSHPVGLVRMGKNVVVGCAQETLHGYTQKGKKLWTTYLPAPVTTMAVLDLPTRGFQAVLVGLANCEVHVYRDKNLISTIKTPDLVTSICFGRYGREDGTLIMTTKGGGLIVKILKRTAVFDDKDSAPGPPIAQSIRLNVPKKTKLYVDQTMRERENAVAMHRAFQMDLSRMRLAAARAYVKALESSLTPMSASLTEPLKMNAVVQGLGPSFKLTLNIQNTAACRPVMNLAISFLYDENLYSMRTAFFKIPLLVPGLNYPIDTFVECLSDKGISDIIKVFVLREGKSAPLLTAHINMPVSEGLALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DY48</accession>
    <name>A0A3B4DY48_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(4) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.67</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 2-oxoglutarate + 3 O2 = L-lysyl(4)-[histone H3] + 3 formaldehyde + 3 succinate + 3 CO2</text>
            <dbReference type="Rhea" id="RHEA:60208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.67" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017537407.1">
        <property type="nucleotide sequence ID" value="XM_017681918.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000029332"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016333.2">
        <property type="protein sequence ID" value="ENSPNAP00000029332.1"/>
        <property type="gene ID" value="ENSPNAG00000015348.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410704"/>
    <dbReference type="CTD" id="327168"/>
    <dbReference type="GeneTree" id="ENSGT00940000157076"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15603">
        <property type="entry name" value="PHD1_KDM5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15687">
        <property type="entry name" value="PHD3_KDM5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000023">
        <property type="entry name" value="Lysine (K)-specific demethylase 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000035">
        <property type="entry name" value="PHD transcription factor Rum1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048615">
        <property type="entry name" value="KDM5_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047978">
        <property type="entry name" value="KDM5B_PHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047979">
        <property type="entry name" value="KDM5B_PHD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF136">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(4) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21323">
        <property type="entry name" value="KDM5_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="22">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="21">
        <location>
            <begin position="80"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="278"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="23">
        <location>
            <begin position="422"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="1142"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="1429"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="178"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1329"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1368"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="1337"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="1388"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1398"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1410"/>
            <end position="1420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1486" mass="168659" checksum="6BB5FB750B48233F" modified="2024-11-27" version="1">MTQPRPSEFIPPPECPVFEPSWEEFADPFGFINKIRSIAENTGICKIRPPPGWQPPFACDVDRLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWELQGCTLKIPHVERKSLDLYQLYKLVTEEGGFDTVCRERKWTQISLKMGFAPGKAVGSHLRAHYERILYPYYLFQTGASLLSSQKPTLTNDTKDKEYKSHDLPQRQSMQPSEISTIARRAKRTEGRCFKSEQGTHCENRPNLRRRMGSYVAKPEPVKNIPVQVKQEPVDPAESTPEGQKSKVEQYICLVCGGGGDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECGKPQVAFGFEQAPRDYTLRSFGDMADSFKSDYFNMPVHMVPTELVEKEFWRMVSTIEEDVTVEYGADIASKDFGSGFPVKNGTFTVTPDEEHYLTSGWNLNNMPVLDASVLTHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGYAAEQLEMVMKKLAPELFESQPDLLHQLVTIMNPNLLMEHGVPIYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWIPLGRQCVEHYRSLNRYCVFSHDEMVCNIAAKADSLELELACAIQKDMSAMIQEEKELREKAYKLGVWHSQQVDYEEQPDEERQCVKCRTTCYLSAITCPCRPDQLVCLHHLQGLCSCDPSNYTLNYKYTLGALNQMFKAVGTRAESYDDWASKVNKILKTDQNKSDLEDLRALVVEAEKKTYPEMDLLRHLRQIIQDAEKSASMAQQLLNGKRQTRYRTGGGKSQNQLTVEELKSFIQQLYDLPCTVRQAPFLKALLSRIEQFQQRSADLLAEDMPGSVALQGLLDEGTGLDVDLPELAVLRQRLEQARWVEAVQQASEQPASLSLDCMRRLIDQGVGLAPHACVERTMAWLQELLTVCEHWEEKAHNMLTARPRHNLEMLEAALLEINSIHAYLPNCLQLKDAVSKAREWIREADALQLGGRVPVLATLSDLVSKAKGIPVLLESLARLESLVSEVQAWKESAVKTFLLKNSPLSLLEVLCPKCETECPKSKSKRVKDVSPSGKKTGVKLDCLSDVEKALSESKDTASAMATLGEVRLKELESLSVLRTSNEAKFQPTPNSSASTVCLCHTVPAGPMLQCELCRDAYHSGCVPGFQITQVGQLWLCPRCSRSKKPPLDKVLPLLASLQRIRVRLPEGDALRYLIERTVRWQHRVQQVSPFTQQFSGKIKPISGMASTLTLEGSNGSFYMLHPCIPLSVLSPDQEELLAEGLLLQVTLPELQQLYQGLLSWFSPSHHSFQSSEHDHQYYIAQDRSPPHRSPPHKSPLHKSPPHSKGQDAVLELQKEREKLEKNHKRRFENENPDVQDRDKIKKPKKKKPKMNKERRREEKRNLSPSNSLSDPSCSEDSEEDWSVCSAKQCLQPEGNEVNWVQCDGSCNQWFHQICVGVSAEQAENEDYICVNCSLNDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DYX1</accession>
    <name>A0A3B4DYX1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">B3GALT2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DYX1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029607"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016852.2">
        <property type="protein sequence ID" value="ENSPNAP00000029607.2"/>
        <property type="gene ID" value="ENSPNAG00000035670.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155117"/>
    <dbReference type="OMA" id="TGNNMRG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045821">
        <property type="entry name" value="B3GT2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF19">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19341">
        <property type="entry name" value="B3GALT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Beta-1,3-galactosyltransferase 2 N-terminal" evidence="15">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029607.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="328" mass="37596" checksum="E2D3EFBB18382FEE" modified="2024-11-27" version="2">MWTEEATSPFPATSEGNLSSILEDSSQQGLKNSVESNVSFSEEIIAESVLKVEPYNYIINEEDKCQEKDPFLVLLITVEPQNNQSRDAIRQTWGNESVAEGVGFVRLFLLGIREGPTSQLQSSIEAESQQYHDIIQQNYIDSYYNLTIKTLMGMHWIATYCPGASYVMKTDSDMFINTEYLIQKLLNPSLPPKLKYFTGRLLKDEPPIRKNDSKWYISEEVYPDDMYPPFCSGTGYVFSGDLAELIYRTSLSIRRLHLEDVYVAICLAKLKIEPVPPPNKSLFNFNRVPYSSCKYSNLITSHGLLPTEIIKYWQDLQTNKHNEHYFGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3R2</accession>
    <name>A0A3B4E3R2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3R2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018284.2">
        <property type="protein sequence ID" value="ENSPNAP00000030333.2"/>
        <property type="gene ID" value="ENSPNAG00000033581.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="OMA" id="ANRTHSY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5046101204" description="Ig-like domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="21"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030333.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="178" mass="19948" checksum="3C63C880DBC4B6D0" modified="2025-10-08" version="3">MLKPIKHYFCLLFLLIHFKGEKIHHPVTEMVIKTGDSATLQCSVQSLNSNCSGDHSVYWFRHGSGESHPGIIYTHGNRSDECEKSSETDSPTQSCVYKLPKRNLSLSDAGTYYCAVAACGRIVFGNQTKVNVQGETLDRIELLFCNGIFLACNRVHHIHSFTSHEYRSVLESAVNILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E638</accession>
    <name>A0A3B4E638_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KCTD13</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BACURD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537257.1">
        <property type="nucleotide sequence ID" value="XM_017681768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E638"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031225"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020133.2">
        <property type="protein sequence ID" value="ENSPNAP00000031225.1"/>
        <property type="gene ID" value="ENSPNAG00000005017.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410609"/>
    <dbReference type="CTD" id="253980"/>
    <dbReference type="GeneTree" id="ENSGT00950000183143"/>
    <dbReference type="OMA" id="GCRIPMI"/>
    <dbReference type="OrthoDB" id="2333377at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021952">
        <property type="term" value="P:central nervous system projection neuron axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061351">
        <property type="term" value="P:neural precursor cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000046">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF26">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="43"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="336" mass="37174" checksum="DEA1F749B9BDE3E1" modified="2018-12-05" version="1">MSAEASSGRVVSEPSPVPPSPNPSPSPSHGPEQKIGGGLAGSKYVKLNVGGSLHYTTVQTLSKEDNLLRSMCDGSTEVTIDSEGWVVLDRCGRHFPLVLNFLRDGTVPLPETQRELEEVLNEAQYYRLQGLIQHCLSTLQKRSEVYEGGCHIPMITSAKEEQRMISTCRKPVVKLQNNRGNNKYSYTSNSDDNLLKNIELFDKLGLRFNGRVLFIKDVLGDEICCWSFYGDGRKIAEVCCTSIVYATEKKQTKVEFPEARIFEETLNILIYENGRSSGHGGMALLESGSVSSPGVGQSEEDGGGVGGGDRRVRRIHVRRHIMHDERGHGQQTVYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAE4</accession>
    <name>A0A3B4EAE4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EAE4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032673"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037478.2">
        <property type="protein sequence ID" value="ENSPNAP00000032673.2"/>
        <property type="gene ID" value="ENSPNAG00000020781.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163991"/>
    <dbReference type="OMA" id="RTRDRCF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="673"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="447"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032673.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="717" mass="80046" checksum="E6DD9C8C3115AB1A" modified="2024-11-27" version="2">MNLRWLRIARAMEFAIHEINNRTDVLPGITLGYQIHDSCSSVPMTVEVAFQFANGVEPFFSDTDSCSKSAAAAVPALHFGWTWIGAVRSDSDYGNYGMASFLKAAQEEGICVEYSESYYRTEPRSKLERVANVIRRSTARVIVAFLHSGDMKFLLEELARQAPPPLQWIGSEAWIIDPDLLRFNMCAGAIGFGIPRSVIPGFREFLQDLSPGQALKSPLLTEFWESSFSCSLKGSTGGARECDGSEDIRALQNPTDTSQLRESNLVYKATYAIAHAIHGVICNDTQCDQRANLFNIKLDVPLQVLDQLKRVNFTTKNGFQVSFDSNGDPVAVYELINWQFKKDVLTKKNILFAKNGDYEALNNLQLSNVFEVPRSVCSESCPLGTRKAVQKGKPVCCYDCIPCAEGEISNKTDSLNCVHCPPEFWPNTQRDNCLPKPVEFLSWDDTLSIILTAFSISGAFIAVCVAVVFYKHRTSPIVRANNSELSFLLLFSLTLCFLCSLTFIGRPSEWSCMLRHTAFGITFVLCISCVLGKTIVVLMAFRATLPGSDAMKWFGPPQQRLSVLTFTLIQVLICTLWLTISPPLPFKNLKQYKEKIILECGLGSNTGFWAVLGYIGFLALLCFILAFLARKLPDNFNEAKFITFSMLIFCAVWLTFIPAYVNSPGKFTVAVEIFAMLASSFGLIFCIFASKCFIIMFRPEQNTKKHLMESADIQKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EBW8</accession>
    <name>A0A3B4EBW8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017557672.2">
        <property type="nucleotide sequence ID" value="XM_017702183.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EBW8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034032"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025531.2">
        <property type="protein sequence ID" value="ENSPNAP00000034032.2"/>
        <property type="gene ID" value="ENSPNAG00000023044.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430037"/>
    <dbReference type="GeneTree" id="ENSGT00940000161625"/>
    <dbReference type="OMA" id="QHPELWT"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08616">
        <property type="entry name" value="PI-PLCXD1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042158">
        <property type="entry name" value="PLCXD1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF24">
        <property type="entry name" value="PI-PLC X DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26146">
        <property type="entry name" value="PI-PLC_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="23"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034032.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="332" mass="37808" checksum="5A02A36F158B5B29" modified="2024-11-27" version="2">MADMERRWDASSCTDWMSQLPPQLLDVPLWDLAIPGSHDTMTYCLDQRSSVLRSQPGILRVLDHVAPCIIRPCVNKWGTTQELVISTQLDSGIRFLDLRIAHKTNDSDQTFYFAHGIYSLVTVKEALTAIAYWLEQHAKEVVIIALSAFDDVNPAQHSKLIDFLKSLFDKKFCPQTEVPTLKACWTRGYQVILSYDDSAGSGHMELWPGWDYWWANESDPNLVISYLEKKKDTVGRPAIFFVAGLNLTEDTKYVLRHLCQSMKSMTLRAYGQLLDWVKQQRPGRQKTCLNVICADFVGISRNEFTQLVIALNTKLLKEKSSGEPTEKNTVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EH17</accession>
    <name>A0A3B4EH17_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reelin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">REELD1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EH17"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026388.2">
        <property type="protein sequence ID" value="ENSPNAP00000034501.2"/>
        <property type="gene ID" value="ENSPNAG00000023829.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163277"/>
    <dbReference type="OMA" id="DMQPKHI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08544">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4060">
        <property type="entry name" value="Reeler domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051237">
        <property type="entry name" value="Ferric-chelate_Red/DefProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002861">
        <property type="entry name" value="Reeler_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042307">
        <property type="entry name" value="Reeler_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45828">
        <property type="entry name" value="CYTOCHROME B561/FERRIC REDUCTASE TRANSMEMBRANE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45828:SF51">
        <property type="entry name" value="REELIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02014">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51019">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043994408" description="Reelin domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Reelin" evidence="4">
        <location>
            <begin position="19"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034501.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="408" mass="45106" checksum="7E2FB3A48140463C" modified="2024-11-27" version="2">MKMVMKAVLLGCVWVSVWPTWCVCFSRGANSASCVDMKPGHISAQSQHTHIQSAVTVHTSRTVYLPQQTLMVSVRSSRAFMGLLLQARSVPDDRVVGGEFTLNPPGTHTLSCISTADTITHSDKLLKRNLSFTWRAPAQPSGDMRFYITVVHSYFVYWSRIRSAVVHDGTRRSPGYDAGLHTRIISGNDTPPIHTHTHTHTHTNVLKSGLFIFFFFTASLLKPVDTQTHTSSDLHSLNSAHSSAIYTNKSSPNTLSPQHTHTTLLTLQRSLSNPFPPRESQDESDSHTQREEGSSEPKPPQRPRGQPRAPDGKVEKPELVPRQNASDLGLLLVGLSAGLGMAVAVGLRYLQRKHCRRHTAMSLGDQSHGNRGVLHVQECGDLVQVRRIRENSFLLLQAEYNLIPAPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHK3</accession>
    <name>A0A3B4EHK3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHK3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035340"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028168.2">
        <property type="protein sequence ID" value="ENSPNAP00000035340.2"/>
        <property type="gene ID" value="ENSPNAG00000025227.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182982"/>
    <dbReference type="OMA" id="FFEEDTC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049885">
        <property type="entry name" value="MTCL1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742">
        <property type="entry name" value="GIRDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742:SF2">
        <property type="entry name" value="PROTEIN SOGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035340.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="160" mass="19996" checksum="981110621DBBBCD6" modified="2024-11-27" version="2">MQEEAAEARSSASRQDEALLREEVDKLLEENEELKAEIEEMRAEMDEMRDSFFEEDTCQLEEVRRELERANKNCRILQYRLRKAERRRMRYAQTGEVDEGALRSLEQDLKVRERDRERERQRVRERERDRERERERETERETERERETETERERDRERDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKC6</accession>
    <name>A0A3B4EKC6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Family with sequence similarity 163 member B, genome duplicate a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FAM163B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM163 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EKC6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030439.2">
        <property type="protein sequence ID" value="ENSPNAP00000036228.1"/>
        <property type="gene ID" value="ENSPNAG00000004137.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412648"/>
    <dbReference type="GeneTree" id="ENSGT00940000159387"/>
    <dbReference type="OMA" id="TRSHRFC"/>
    <dbReference type="OrthoDB" id="9887724at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029379">
        <property type="entry name" value="FAM163"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040280">
        <property type="entry name" value="FAM163B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31396:SF2">
        <property type="entry name" value="PROTEIN FAM163B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31396">
        <property type="entry name" value="PROTEIN FAM163B MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15069">
        <property type="entry name" value="FAM163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="42"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="21036" checksum="FEEDF4B693E0B441" modified="2018-12-05" version="1">MTAGTVVITGGIIAAVILLTIVTVLCYCRLQYYCCKREGSEGEEEEPDFTKSSPSPPQELNPSPPSSPSPPSPLSFHPPPEYSSSNQLLMTAEPYEPNGPAICSRYSPTVPRRPIRSYTFCPSCSGYLPFYLNPQEGLRNGGGRISYRTVQQQELDLPVVPVDMPNFNKLNLIRSVTMREVVTHHSVSTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IHJ6</accession>
    <name>A0AAR2IHJ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="6">M1Pi</shortName>
            <shortName evidence="6">MTR-1-P isomerase</shortName>
            <ecNumber evidence="6">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">MRI1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IHJ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055917.1">
        <property type="protein sequence ID" value="ENSPNAP00000038765.1"/>
        <property type="gene ID" value="ENSPNAG00000012506.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013732"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000004">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="355" mass="37753" checksum="6FF47BEC48370989" modified="2024-11-27" version="1">MTLEAIRYRAGSLQILNQLLLPHETVYEEIRLVQDGYEAIKSMKVRGAPAIAIVGCLSLAVELRAGAGGEDPVGFIRESLCHLTSARPTAVNMGRAARELMEFTENESMEKSGDQLRESVIRCIEEMLERDVNDNKKIGNYGAQHILSGVPRDSVTILTHCNTGSLATAGYGTALGVVRSLHTLGRLKRVYCTETRPYNQGARLTAYEAVAEGFPATLITDSMASLTMREKNITAVVVGADRVVANGDTANKVGTYQLAIAAKHHGIPFYVAAPSTSCDLSLESGRDIVIEERPAEELTSINGVPVAAPGESSINSGYYLSAVNLVVVVVGGGGGGVVMPSLIPLTFTCAVITNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IK88</accession>
    <name>A0AAR2IK88_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Intersectin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="36" type="primary">ITSN1</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="25">Synapse</location>
            <location evidence="25">Synaptosome</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:neuron projection"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
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            <end position="923"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="995"/>
            <end position="1012"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00074966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1742" mass="196148" checksum="60A80D475927AB08" modified="2024-11-27" version="1">MAQFPTPFGGGLDTWVISVDERAKHDQQFHSLTPTPAGFITGDQAKNFFLQSGLPAPVLAQIWALADLNNDGKMDMHEFSIAMKLIKLKLQGHPLPPTLPPSMKQPPLSIPPPSAFGMPSMGTMTGIPAVPPMPLPPLPPGVPGVGMSPPLVSSVTPPVPPMANGAPSMIQPISGFSHPATALNKSSSFNRSSAKLPKGQSFETPSAPAAPVPSDWAVPQSSRLKYRQLFNSHDKMMSGHLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPELLPPTFRRVRSGSGVSMTSMHPIDQRVQEESAEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAALEREEQERKERERQEQERRRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLTQRNREQENIVLLKARKKTLEFELEALNDKKSQLEGKLQDIRFRLSTQRQEIESTNKTRELRIAEITTLQQQLQESQQWLGRLIPDKQCLNDQLKQVQQNSLHRDSLSGLQRAVEMKEATRQQLCEQLDAVEKETRSKLLEIDAFNTQLKELREIHNKQQRQKQKELEGDIIQPPLDSKPADLQESRVLGADDGMSPAWRDSALGKAPTPPASQPWMSHVSEEESQSSRGDMQDKLSKLFMQQPEPGKLLGQSPWPALDKVPISGLNQEKVRVVYYRTLYPFEARSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGEIKGKTGWFPANYAEKIPESEVPLNLRASATSSSASKLGTRITPTSSTTPTPLQPVTTETSAVAPPTTATAAPCPTSSSSSASSNWADFSTTWPTNSAVEKQDADGWEAWPTQPTQPTQPSLSVPSGGQGRQRSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQRGWFPKSYVKLISGPVRKSMSIESGSSDSPPSMKRPSPSPNKPMELGEEYIAMYTYESSEQGDLTFQQGDVITVLKKEGDWWTGTVAGKTGVFPSNYVKPKDSEGLGPAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTSKTTPTEPTPPKLPTPNAVCQVIGMYDYVAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGLFPSNYVKLTTDTDPSQQWCADLHLLDMLTPMERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPDFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQVIKPLGSSASDKVFSAKSHLQYRMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQGSKGPITKRLLLHEVPTGEIVVRLDLQLFDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IT81</accession>
    <name>A0AAR2IT81_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RAB1A, member RAS oncogene family b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IT81"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078474.1">
        <property type="protein sequence ID" value="ENSPNAP00000040826.1"/>
        <property type="gene ID" value="ENSPNAG00000012540.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155078"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000069">
        <property type="entry name" value="Ras GTP-binding protein YPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22691" checksum="2F79533FAB67323A" modified="2024-11-27" version="1">MPPIYLSFDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLEASAKNATNVEQAFMTMAAEIKKRMGTGANAGGSEKSNVKIQSTPVKTSTGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IU31</accession>
    <name>A0AAR2IU31_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aquaporin 8b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IU31"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053312.1">
        <property type="protein sequence ID" value="ENSPNAP00000043253.1"/>
        <property type="gene ID" value="ENSPNAG00000010954.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165638"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000019">
        <property type="entry name" value="AQuaPorin or aquaglyceroporin related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023277">
        <property type="entry name" value="Aquaporin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF11">
        <property type="entry name" value="AQUAPORIN 8B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02020">
        <property type="entry name" value="AQUAPORIN8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="27479" checksum="74A81EAA445CD563" modified="2024-11-27" version="1">CPMEEEKMELKEVDRSSQERAKKAAKPPSMFERMIQPCIAELVGTMFFVFIGCVSVIENVEAAGRLQPALVHGLAVAVLVACMAEISGSHFNPPFTIGIFLCGGIQMSLAVLYLVCQLIGGVLGAAMSKVMTSKENYMNATGAAFTILKSDDQLGAAIFAEMAMTCLVTMVVLLGAVNGKSKSPLVPFMVGCTVIINILAGGDVSGTCLNPARALGPALMAGYWTYHWVYWVGPIGGGLIAAALVRLLLGDEKIRVIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IY01</accession>
    <name>A0AAR2IY01_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Krueppel-like factor 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Interacts with FBXO38.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558516.1">
        <property type="nucleotide sequence ID" value="XM_017703027.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IY01"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058932.1">
        <property type="protein sequence ID" value="ENSPNAP00000044618.1"/>
        <property type="gene ID" value="ENSPNAG00000030122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430501"/>
    <dbReference type="CTD" id="553082"/>
    <dbReference type="GeneTree" id="ENSGT00940000155235"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21585">
        <property type="entry name" value="KLF7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000021">
        <property type="entry name" value="Basic krueppel-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001395">
        <property type="entry name" value="Krueppel-like factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF77">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="211"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="241"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="81"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="294" mass="32427" checksum="8FDBCF7377DD598A" modified="2024-11-27" version="1">MDVLANYSIFQELQLVHDTGYFSALPSLEENWQQTCLELERYLQTEPKRFSDLFDEELDCLLTPAFMKRDSDEDLMDPLLPGLADEPSPPPPLLVSLPKSLHAPASGKPTDAGAKEGGVNPAQLSAVTSLTPPSSPELGRHLVKPTQTLSATADGTLTLKLVAKKVGLGAAKLVATHSLPSPPSRGAQSDGEGGVGSVGVSDIPENKKRVHRCQFNGCRKVYTKSSHLKAHQRTHTGEKPYKCSWEGCEWRFARSDELTRHYRKHTGAKPFKCNHCDRCFSRSDHLALHMKRHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0N7</accession>
    <name>A0AAR2J0N7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease 3</fullName>
            <ecNumber evidence="7">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Ribonuclease III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24 25">protein Drosha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">DROSHA</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="23">Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region). Interacts with SP1 and SNIP1. Interacts with SRRT/ARS2. Interacts with CPSF3 and ISY1; this interaction is in an RNA dependent manner. Interacts with PUS10; interaction promotes pri-miRNAs processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="7"/>
    <dbReference type="RefSeq" id="XP_037400369.1">
        <property type="nucleotide sequence ID" value="XM_037544472.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037400370.1">
        <property type="nucleotide sequence ID" value="XM_037544473.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000042329.1">
        <property type="protein sequence ID" value="ENSPNAP00000045563.1"/>
        <property type="gene ID" value="ENSPNAG00000011876.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431366"/>
    <dbReference type="GeneTree" id="ENSGT00730000111052"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070877">
        <property type="term" value="C:microprocessor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070878">
        <property type="term" value="F:primary miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19877">
        <property type="entry name" value="DSRM_RNAse_III_meta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000002">
        <property type="entry name" value="Drosha ribonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000003">
        <property type="entry name" value="Drosha ribonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000012">
        <property type="entry name" value="Drosha ribonuclease III"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
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    <dbReference type="InterPro" id="IPR058938">
        <property type="entry name" value="Helical_CED_Drosha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044442">
        <property type="entry name" value="RNAse_III_DSRM__animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26050">
        <property type="entry name" value="Helical_CED_Drosha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="18 26" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="29">
        <location>
            <begin position="818"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="29">
        <location>
            <begin position="1049"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="28">
        <location>
            <begin position="1202"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="392"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1296"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="221"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="304"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="27">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1296"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="27">
        <location>
            <begin position="1307"/>
            <end position="1316"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00017706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00055411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00063453"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00078955"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1316" mass="152393" checksum="F3DFC6AE19C463C1" modified="2024-11-27" version="1">MSFHSGRGCPRGRPPPGQHGSPQNYRPTGLRLPTPQPPMPPYPYDPPAPHCSGYSVQGANSYMPPRPEFMPYPIPALSQGPQSQCPIRPPFPNPPLRQGFPEPPNFPPPPLPGSSTNVAQSQGSNPYMISSVPPPPLPPVPPSMPPSMPYQPPYSMSYPPQPHFPPPPTFNPGYPQPEGSSFKPEHFPRHPPQYKGEKSRSPERPRRHEEHRHKSYGYSGHGERHKAEYGGERKDRGRSPEVQRRRTERYRSEHERGRTPPRHRSRERSRERYRHRDSRSSPSSDRHRKRPRSRSASRERKRGRMEEERERRSEGGNRERSYTSTRSSRESEEISSDQRHDEEKEEEELLKPAWIRCTHAENYYSNDPMDQVGDSTVVGTSKLRDLYERFEEELGRRQERAKSSRPKWEPPKTKLDLDQDESSSESECDSDGDSSCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEPVKQCRPMNNNAGKLFHYRITVSPPTNFLTDRPTVIEYDDHEYLFEGFSLFSHTPLTNIPLCRVIRFNIDYTIHFIEEMSPENYCVKGLELFASYLFQDILELYDWNLTGPEHSDPPGCQRFHFMPRFVRFLPDGGKEVLSMHQVLLYLLRSNKPLVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGMKPSSVRIDQLDREQFNPNVITFPIIVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQIDKQKLTQREEALQKIRQKNSMRREVTVELSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFKERCLLQLGMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDRKVHHMYMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTSVHLFYLFPNLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAVYLEGGLEEAKQLFGRLLFNSEGLREVWLNYPPHPLQVQEPVTDRQLIESSPVLQKLTDFEKAIGVLFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAISNDKTKRPVALRTKTLADLLESFIAALYIDKDLEFVHTFMNVCFFPRLKEFILNQDWNDPKSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEKYNFPQMAHQKRFIERKYRQELKEMRRERERQERESDEGEDGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J6P8</accession>
    <name>A0AAR2J6P8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 4E family member 1B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">EIF4E1B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J6P8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059362.1">
        <property type="protein sequence ID" value="ENSPNAP00000046017.1"/>
        <property type="gene ID" value="ENSPNAG00000000195.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160838"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF75">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26578" checksum="6290D57381BE9D05" modified="2024-11-27" version="1">MAACAVQLIDKQPRKKFEKKEAPMVPVVKEPQARHPLQNRWALWFYKNDKSKMWQDNLRLITKFDTAEDFWALYNNIQPASKLSSGCDYSVFKDGIEPMWEDRSNKCGGRWLITLSKQQRHSELDRFWLETLLCLIGEGFGSYSRDVCGAVINVRAKGDKIAIWTTNTENAEAVTYIGCAHVVGCTNMTVDFATSVNRKYKETLGLPAKLVIGYQAHADTATKSNSITKNKFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J8E1</accession>
    <name>A0AAR2J8E1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J8E1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054819.1">
        <property type="protein sequence ID" value="ENSPNAP00000048298.1"/>
        <property type="gene ID" value="ENSPNAG00000030186.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000283118"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="155" mass="16957" checksum="517513BB27869CCE" modified="2024-11-27" version="1">YSNTSSKHTNTSSKHTNTSSKYSNTSSKYSNTSSKYSNTSSKHTNTSSKHTNTSSKYSNTSSKHSNTSSKHTNTSSKHTNTSSKYTNTSSKYSNTSSKHSNTSSKHINTSSKHTNTSSKHTNTSSKHTNTMSVLLCVFRVRSVSVLLCVCLEYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JBY1</accession>
    <name>A0AAR2JBY1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017579868.2">
        <property type="nucleotide sequence ID" value="XM_017724379.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037387981.1">
        <property type="nucleotide sequence ID" value="XM_037532084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JBY1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086342.1">
        <property type="protein sequence ID" value="ENSPNAP00000047346.1"/>
        <property type="gene ID" value="ENSPNAG00000036109.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108443621"/>
    <dbReference type="GeneTree" id="ENSGT01150000286911"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000538">
        <property type="entry name" value="Si:ch211-127b6.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000037">
        <property type="entry name" value="Si:dkey-191j3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="9">
        <location>
            <begin position="289"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="1046"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1239" mass="139375" checksum="1C396395D2783936" modified="2024-11-27" version="1">MMELQLSSSGGGTLDPKFSDVGYPEPSCMSMKSDGSMRNSPVFSSGGVEPSCASMKSDGSMRNPPVFSSGGVEPSCASMKSDGSMRNPPVFSSGGVEPSCASMKSDGSMRNPPVFSSGGLEPSCASMKSDGSMRNPPVFNSGAIPSDPDQRSTEATLKINTQLEGSGDLHQDSYQPMDDDLLKVLERHKTNMKNKYESLFEGIKTEKNKTLLNRIYTQLYIIEGESEGVNEEHEVLQMEKTSRKHQQDTPINCLDIFKPQYLKDGQIVVARAEYLRQKEGQELNVQKLKAVLTKGIAGIGKTVSVQKFILDWAEGKANQDVDVMFVLPFRELNLIKDDQYSLHGLLCDFHPELKDLDPEIYDQIKAVFIFDGLDESRIPMNFSRSKKVSDITMTSSVGVLMTNLIKGELLPSALIWITSRPAAANQIPPKYINRLTEIQGFSDPQKEEYFRKRISDEDQAKKIISHIKTVRSLHIMCHIPVFCWISATVLQRIMKQPHTEIPKTLTEMYSHFLITQTNMKNEKYEEKDESDPKNLLESNRTILLKLAELAFKQLMKGNVMFYEEDLRECGIDVTEASVYSGIFTEIFREECVVFQRKVYSFVHLSFQEFLAAVYVFHCYESKNMKALQIFKPRYSEWSDNVLLGFFNPRYRDWSDDVPLVEVLNGAVNKAVKCQNGQLDLFLRFLLGISLETNQRLLQGLPTPIKSSSEKIIEYIKYLIKGDDYQQDHISTERSINLFLCLSEMNDQSLSRELHEYLKSERHSEEKLSPGQCSALAFMLLSSEEVLDELDLKKYSKSVEGCRRLIPAVSICRKARIAGCNFTKDSCEMLCSALQSVKYLKELDLSNNTFQDSGVELLSAGLKSTHCKLETLRLASCNLTKVSCESICSVLQSTNCLLKELDLNNNKLLDLGVELLTAGLKRSHCKLETLRLTLCNLGEKACENLGSVLQSSNSSLKELDLSNNDLQDSGVQLFSTGLQSVNCKLEKLRLSGCMFKDEGCSSLVSALKLNPSHLRELDLTYNHPGKSGVKLLSDLLEDVHCALDTLRVDHGGVIRMKPGLKKYAVGLTLDPNTVHTRLSLSEGNRKVERAREELPYPDHPDRFEYRDQVLSVESLTGRCYWEVQWSREAVIAVSYRGIKRKGHSDDCMFGNNNQSWCLICYNNSYSVRYNKQISPVPSPPSPSNRVGVYLDWGSGTLSFYSISSDTHTLTHLHTLTTTFTEPLYAGFCVYDDSVQLPEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JE96</accession>
    <name>A0AAR2JE96_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DDB1- and CUL4-associated factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DCAF11</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 7">May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with DDB1 and CUL4A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat LEC14B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551689.1">
        <property type="nucleotide sequence ID" value="XM_017696200.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JE96"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077055.1">
        <property type="protein sequence ID" value="ENSPNAP00000048181.1"/>
        <property type="gene ID" value="ENSPNAG00000024812.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426587"/>
    <dbReference type="CTD" id="80344"/>
    <dbReference type="GeneTree" id="ENSGT00720000108873"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000115">
        <property type="entry name" value="DDB1- and CUL4-associated factor 11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000492">
        <property type="entry name" value="LEC14B homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051859">
        <property type="entry name" value="DCAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017399">
        <property type="entry name" value="DCAF11/LEC14B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19847">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19847:SF7">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038135">
        <property type="entry name" value="WD_repeat_p23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="311"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="359"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="487"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="545" mass="61300" checksum="287017B7208334F5" modified="2024-11-27" version="1">MGSQSSSGMSGRGSSGNPDQSEPESNQNSTQRGRRQSDRQTERVTASEEDVDLAQVLAYLLRRGQVRLVHGSGATGLQLVQSVSDSDEDSDGAWEGRLGHRYDPPVDSHPDTQDVDRSEIRTQILLATASSNLKGRHSFTHMLAQREQGRCRGSSFSHGECSRIRSHFLPNFVAHKDTYQQKAFCGVYSDDGNLFLSACQDQNIRLYDTSRGGFMLKRTVKARDVGWSVLDVCFTPDSRSVLYSSWSDYIHVCSIDGESETHTALDLNPEERRFCVFSLAASTDGKEILGGANDGCLYVFDREQNKRTLKIDAHEDDVNAVAFADSSSQLLFSGSDDALCKVWDRRTLREDRPQPVGHLAGHRDGITFIHSKGDARYLISNSKDQTIKLWDVRKFSPKEGLAASRLAVTQQNWDYRWQQVPQRALKRHKLTGDTSVMTYRGHGVLHTLIRCRFSPEFSTGQRFIYTGCSTGKIIIYDVLTGCVVSKLAGHDACVRDVSWHPFQDNIISSSWDGAVRLWEHRQSHLLDEESDRPREWIRGIEEKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JFW1</accession>
    <name>A0AAR2JFW1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein spinster homolog 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Spns1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(out) + H(+)(out) = a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74447"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(out) + H(+)(out) = a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74455"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine(out) + H(+)(out) = a 1-acyl-sn-glycero-3-phosphocholine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74435"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphoethanolamine(out) + H(+)(out) = a 1-acyl-sn-glycero-3-phosphoethanolamine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:74439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64381"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JFW1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074756.1">
        <property type="protein sequence ID" value="ENSPNAP00000049212.1"/>
        <property type="gene ID" value="ENSPNAG00000003183.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005976"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17328">
        <property type="entry name" value="MFS_spinster_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF13">
        <property type="entry name" value="PROTEIN SPINSTER HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="425"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="17">
        <location>
            <begin position="105"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="561" mass="62481" checksum="E6C40E9D31635197" modified="2024-11-27" version="1">MFLLSLLRYIYMYFHYVASRVRCPYLLPLAMYTHRHFPFSSVLHLLNRSDHAHFRIIGSSETFLNEEHTTVPFPPQWSAEKDDRPSPEQEEDSPSGLSHIRAHLTVVVLCYINLLNYMDRFTVAGVLPDIEHFFNISDGKSGLLQTVFICSYMILAPLFGYLGDRYNRKMIMCVGIGFWSVVTLASSFVGKDYFWALLLTRGLVGVGEASYSTIAPTIIADLFIKEKRTNMLSIFYFAIPVGSGLGYIVGSAVDNRAKDWHWALRVTPALGLVAVLLLVCVVKEPKRGAIEARPEHTLQRTGWLADMKALCKNNSFVLSTFGFTAVAFVTGSLALWAPAFLFRAAVFTGEKQPCVVEPCDTSDSLYFGIITCVTGVLGVAMGVGVSQWLRKRTPRADPLVCAAGLLLAAPFLYLSIIFAQKSTVATYFFIFFGEMFLCMNWAIVADILLYVVVPTRRSTAEAFQIVISHLLGDAGSPYLIGVVSDSLKQADSYMWQFRSLQLSLLLCSFVAVVGGAFFLATALFIEKDRQIAENYVQAGDCDRGVTPKACMLVVFPIFYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JKF1</accession>
    <name>A0AAR2JKF1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27429"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27430"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017553396.1">
        <property type="nucleotide sequence ID" value="XM_017697907.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391043.1">
        <property type="nucleotide sequence ID" value="XM_037535146.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391044.1">
        <property type="nucleotide sequence ID" value="XM_037535147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JKF1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071784.1">
        <property type="protein sequence ID" value="ENSPNAP00000050679.1"/>
        <property type="gene ID" value="ENSPNAG00000029841.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427627"/>
    <dbReference type="GeneTree" id="ENSGT00940000160046"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031703">
        <property type="entry name" value="Lipin_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF62">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE LPIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16876">
        <property type="entry name" value="Lipin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="7">
        <location>
            <begin position="844"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="114"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="490"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="581"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="729"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="731"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1054" mass="116089" checksum="5718B39B90E2748E" modified="2024-11-27" version="1">MNYVGQLAETVFVTVKELYRGLNPATLTGGIDVIVVRQPDGSLQCSPFHVRFGKLGVLRSKEKVVDIEINGDPIDLHMKLGDNGEAFFVEENENLEAAVPAHLCTSPIPTEIPEISEVAESPSSTTLFSSNRRKKRRRRRARSDAHLHEDGSSSSEERETPEQDILNEESLSPLLASKSVYYSLSEEQSEELTGGQTRVSHPHSDGECSPNESVFFSRPSSPKSDSELLVKPQETSGPQMQWNWGGFPKVCQAEQPRTPSSPSIPSDHSYFRTIQRQASFDTEPCLETETVTVVRPEPWTFVTEPCPSDNLPSVSHSTPNNTSGTSSPTHQISPLMLPLVETLQPVDCSDQLPVSGLGRQDTNDAMTNYTANPVSTCNTDSDDRIANPGNSANTTSYANAVGLSSITHTISTNVNTNAVVSDCPGSQLNRIVKISLAATAGSVAGVSVGASETIATSVSTINAFSEAAHQSTTERAVFVERDSGIEPFAEGLAEEDGEVSMESPPTRQDAESAEVACPVESVIQLEEQPGFSQPPSKAVELQDKKKGKRSQHLGPADIYLDDLSNLDPEMVALYFPRSDSESASRSGAELSSVHPPRVSPQSGQQSPQSLGSGNVDSGTDYLSDATTDSLDVTMSLCGTEGHSRQINKEKFMAHIVKYQDLVSNPGIIDDPNLVFCINSNYYNWAVAAPMILSMQAFQKNLPKSTVEQLIKDKMPKKSGRWWFSWRRRDMNSNQPSSKPDSNGNQLETSSARPVPPLLKDHSGQLSSDDDGTDSIRQKTIAETMTTAQCLSQMYRKSLRLTSQQIERLNLREGANKIVFSVTTQYQGTCRCEAAIYLWNWDDKVIISDIDGTITKSDALGHILPQFGKDWTHQGIAKLYHKIHQNGYKFLYCSARAIGMADITKGYLQWVNDKGIVLPKGPVLLAPSSLFSALHREVIEKKPEVFKITCLADIRDLFSPQRYPFYAAFGNRINDVFAYKKVGVPETRIFTVNPKGELIQENSKGYKSSYSHLSELVEHVFPLLCKGRPTSFDYPEYSHFSYWREPLPELDFSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLS7</accession>
    <name>A0AAR2JLS7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Acid sensing ion channel subunit family member 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homotrimer. Heterotrimer; with other ASIC proteins producing functional channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037394990.1">
        <property type="nucleotide sequence ID" value="XM_037539093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLS7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062031.1">
        <property type="protein sequence ID" value="ENSPNAP00000051252.1"/>
        <property type="gene ID" value="ENSPNAG00000023562.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443384"/>
    <dbReference type="CTD" id="407668"/>
    <dbReference type="GeneTree" id="ENSGT00940000159052"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.470.10:FF:000001">
        <property type="entry name" value="Acid-sensing (proton-gated) ion channel family member 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000001">
        <property type="entry name" value="Acid-sensing ion channel subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.470.10">
        <property type="entry name" value="Acid-sensing ion channels like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF13">
        <property type="entry name" value="ACID-SENSING ION CHANNEL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 17" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="12 17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="61291" checksum="1D00DCCC7CC7DB85" modified="2024-11-27" version="1">MPIEFVCKIKFAEEDEKQKKGKKQQDGDRESLIEESCSPPAKDLAGFANSCSLHGINHIFVSGRLGVRQTLWALAFLASLALFLYQAAKCAISYLEHPHVTALNEEASPEMVFPAVTICNINRFRFSALTDADIYHLANLTGLPPKNKDGHKPTDLEYPAPDMQDIFNRTGHQLDEMLKSCNFSGQNCSAEDFTVVYTRYGKCYTFNGNKTTSRKTKQGGMGNGLEIMLDIQQDEYLPIWRETNETSLEAGIRVQIHSQDEPPYIHQLGFGVSPGFQTFVSCQEQRLTYLPQPWGNCRSSTEQIIPGYDTYSISACRLHCETRVVLQECHCRMVHMPGNADICTPNKIKCVDRALALLQKSSGDSCPCETPCNLTRYGKELSMVKIPSKGSARYLSRKYHKSEDYIRDNFLVLDIFFEALNYETIEQKKAYDVAGLLGDIGGQMGLFIGASILTVLEILDYIYEVIKHRLQRLLKPQKEQKPQPQQKQPPASTVATVNLEDMKAKDGSERGNSEGAYANTILPNHHHHLHLHHSRHTPFEDFAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JMM0</accession>
    <name>A0AAR2JMM0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KLC2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JMM0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087921.1">
        <property type="protein sequence ID" value="ENSPNAP00000053265.1"/>
        <property type="gene ID" value="ENSPNAG00000001947.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166677"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF2">
        <property type="entry name" value="KINESIN LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
        <property type="entry name" value="KINESIN_LIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="239"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="527"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="587"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="85"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="634"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="648" mass="72510" checksum="A73E2A02A58CE28D" modified="2024-11-27" version="1">MSTMVYPREEALERLTQDEIVLNTKAVMQGLETLRGEHAQLLNSLLDCAQPPAAQEKSGLLRKSLEAIELGLGEAQVIIALSGHLSAVESEKQKLRAQVRRLCQENQWLRDELANTQHKLQKSEQNVAQLEEEKKHLEFMNQIRKFDDDGSPSEEKPGEKEKDNLDDLFPNDDDQGPAQPSGEVAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKYHPDVAKQLNNLALLCQNQGKYEEVEYYYRRALEIYESKLGADDPNVAKTKNNLVRAEPQLLTARLEDLISLEIHNRFFFPWLYQATCYLKQGKFKDAEALYKEILTRAHEKQFGSVNNDNKPIWMHAEEREESKGKKKDSGPYGEYGSWYKACKVDSPTVTNTLKSLGALYRRQGKLEAAETLEECATKNRKQGIDAINQSKVVELLKDGVAGGDRRQNRDGMNGPGGPRGDSEADEAEWNGDGNGSLRRSGSFGKIRDALRRSSEMLVKKLQGSGPQEPRNPGMKRASSLNFLNKSAEETSQEANSGLSECRGLSASNVDLSRRSSLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JPD3</accession>
    <name>A0AAR2JPD3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">IDH3A</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JPD3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066157.1">
        <property type="protein sequence ID" value="ENSPNAP00000052147.1"/>
        <property type="gene ID" value="ENSPNAG00000031894.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064449"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018154">
        <property type="entry name" value="TLV/ENV_coat_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424:SF80">
        <property type="entry name" value="ENVELOPE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10424">
        <property type="entry name" value="VIRAL ENVELOPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00429">
        <property type="entry name" value="TLV_coat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58069">
        <property type="entry name" value="Virus ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="377" mass="41206" checksum="C9EE44A38A457370" modified="2024-11-27" version="1">MTGFKCMIELTKQANPINCTTLADIFPVIANTTGTGPFRAPLSGNFTCFNHTYVDGTGRGKMIGQINPDWCHITYSGNASVGPWAGAGLYYYCGGTTLLVRMPIHTFGLCAMVRLVSPILLVGQRQAKHITMHRRKRETTEDHFDLTKNSLTYIDAIGVPRGVPDEYKLVDQIAAGFENIPIISALFPVTPNKNVDRINYVNYQVMRLANLTKVAVEGLSAQLSATSLMAIQNRMALDMLLAETGGVCHMFGDMCCTFIPNNTAPDGSVTKALEGLKALSKEMHDNSGLDNAITGWLDKVFGKWKAVFISLCMSIAVFLAILIICGCCCVPCIRTLAERFITSMFHKQEPKGAIMLPLLDSNGRSGDQDSDLENNMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JVL3</accession>
    <name>A0AAR2JVL3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="21">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">NME7</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Possesses an intrinsic kinase activity. Displays 3'-5' exonuclease activity with a preference for single-stranded DNA. Does not seem to have nucleoside diphosphate kinase activity. Functional component of the gamma-tubulin ring complex, implicated in the regulation of the microtubule-nucleating activity of the gamma-tubulin ring complex in centrosomes, in a kinase activity-dependent manner. Part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of sperm flagellar doublet microtubules. Component of the gamma-tubulin ring complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19 20">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JVL3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042948.1">
        <property type="protein sequence ID" value="ENSPNAP00000056093.1"/>
        <property type="gene ID" value="ENSPNAG00000005268.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158946"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04415">
        <property type="entry name" value="NDPk7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04412">
        <property type="entry name" value="NDPk7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.170:FF:000005">
        <property type="entry name" value="Nucleoside diphosphate kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000004">
        <property type="entry name" value="Nucleoside diphosphate kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000010">
        <property type="entry name" value="Nucleoside diphosphate kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006602">
        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057579">
        <property type="entry name" value="DM10_NDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011410">
        <property type="entry name" value="NDPK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035525">
        <property type="entry name" value="NDPk7A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037993">
        <property type="entry name" value="NDPk7B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43109">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43109:SF2">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25364">
        <property type="entry name" value="PH_NDK7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036503">
        <property type="entry name" value="NDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="18 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DM10" evidence="23">
        <location>
            <begin position="25"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="17 19">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="19">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" description="ATP" evidence="18">
        <location>
            <position position="121"/>
        </location>
        <ligand/>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" description="ATP" evidence="18">
        <location>
            <position position="167"/>
        </location>
        <ligand/>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" description="ATP" evidence="18">
        <location>
            <position position="215"/>
        </location>
        <ligand/>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" description="ATP" evidence="18">
        <location>
            <position position="225"/>
        </location>
        <ligand/>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR036503-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR036503-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="401" mass="44964" checksum="FDE9BF602F84E1E5" modified="2024-11-27" version="1">ECWCESSSSSSDQRVHSVFLLCCSQEERFAFLAEWYDPSAALLRRYQLLFYPKDGSVEMFDVKNQRTFLRRTKYDDLHPEDLFVGNRVNVFSRQLNLIDYGDQYTANKLGSKKERTLAMIKPDAVTKVGDIIQMIYDAGLIVTKAKMTKLTWKQAADFYMEHQSKSFFNNLMQFVTSGPVIVMELMGDEAVSVWRRVLGPTDSSVARKDAPNSLRAQFGTDGTKNAGHGSDSLASAARELEFFFPSTAGHGPSNTAKYSDCTCCIVKPHAISEGKTLNTQITHTLIGLCFNGEKAICMFNMDRANAEEFLEVYKGVVTEYTSMVAELCSGPCMALEIHGTDAPREFRAFCGPADPEIARHLRPTTLRALYGRNKVQNAVHCTDLPEDGLLEVQYFFKILDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KA87</accession>
    <name>A0AAR2KA87_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonuclease H</fullName>
            <ecNumber evidence="3">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the taxilin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KA87"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080123.1">
        <property type="protein sequence ID" value="ENSPNAP00000059394.1"/>
        <property type="gene ID" value="ENSPNAG00000029794.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158303"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026183">
        <property type="entry name" value="Taxilin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127:SF12">
        <property type="entry name" value="ALPHA-TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127">
        <property type="entry name" value="TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09728">
        <property type="entry name" value="Taxilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="550"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="257"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="626" mass="73144" checksum="2F60333D4A565773" modified="2024-11-27" version="1">MKVWERVVEARRRREVQISEQQFGFMPRKRTTDTIFALRVLVEKYREGQKELHCVFVDLEKAYDRVPREELWYCMRKSGVAEKNVRVVQDMYEDSETVVRCAVGVTNGFKVKVGLHQGSALSPFLFAMVMERLTDEVRQEAPWTMMFADDTVICGESREQVEENLERWRFALERRGMKVSRDKTEYMCVNEREAGGKVKMQGVEVVKVDDFKYLGSTIQSNGQCRKEVKKRVQAGWSKEITLLMQTLNTLSTPEEKLAGLCKKYAELLEENRNTQKQMRALQKKQTQLIQEKDHLRNEHSKAILARSKLESLCRELQRHNRTLKEDGVQRARQEEEKRKEVTSHFQVTLNDIQAQMEQHNERNASLRQENMELAEKLKKLIEQYELREEHIDKVFKHKDLQQQLVDAKLHQAQALLKDSEDRHQKEKEFLLKEAAESQRMYELMKQQEVHLKQQLSLYTEKFEEFQNTLSKSNEVFTTFKQEMEKMTKKIKKLEKETTMYRSRWESSNKALLEMAEEKTLRDKELESLQVKVQRLEKLCRALQIERNELSKKVQGLSKPEDDGQGVDSGGATEAESQQPAIDSEEGSPAHRNSPEPETAPTTCAQACHCGPELEAEAQREVCSGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJV1</accession>
    <name>A0AAR2KJV1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTPase Era, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ERA-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ERAL1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570388.1">
        <property type="nucleotide sequence ID" value="XM_017714899.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KJV1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065160.1">
        <property type="protein sequence ID" value="ENSPNAP00000064603.1"/>
        <property type="gene ID" value="ENSPNAG00000009960.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437662"/>
    <dbReference type="CTD" id="26284"/>
    <dbReference type="GeneTree" id="ENSGT00390000013800"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22534">
        <property type="entry name" value="KH-II_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002220">
        <property type="entry name" value="GTPase Era, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000016">
        <property type="entry name" value="GTPase Era, mitochondrial isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTPase_Era-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="GTPASE ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698:SF1">
        <property type="entry name" value="GTPASE ERA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Era-type G" evidence="18">
        <location>
            <begin position="100"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="16">
        <location>
            <begin position="108"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="16">
        <location>
            <begin position="134"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="16">
        <location>
            <begin position="155"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="16">
        <location>
            <begin position="224"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="16">
        <location>
            <begin position="319"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="448" mass="49803" checksum="5CE8338769F35BE5" modified="2024-11-27" version="1">MAIRVGESFVRRCLRAPARPLLLATVSLRPRAAFRPGALSGHGFPFTPACSVSSSAFLDRLLKGKMPGVDQGPCQQPVPPDRAGQLSLLLRPPDQPENPKTLRVAVIGAPNAGKSTLTNQLLGRKLFAVSQKVHTTRSRALGVITMDNTQIILLDTPGLTTPFKVKRHQLENSLLVDPLNSLREADLVVVLVDISDKWTRDRLDFEVLKCLTLNPHIPAVLVLNKVDLLKNKALLLDITAKLTEGVVNGQRLRVRDVVKILQKERDEMQMKAKTALEAQTGEEPCPNSDTQDQTGLSREQLRTLKSRKGWSHFKDVFMLCAADGEDVETLKRYLMVAAKPGPWHYHSDVLTDQSPEDICVNAVREKLLEYLPQEVPYTISQHIELWRETEDGKLDISVKLHVKKNSHMTMVIGPGGQVMARIAREAGEDLTNAFMCEVRLKISATLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKG2</accession>
    <name>A0AAR2KKG2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">adenylate cyclase</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 23">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKG2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086286.1">
        <property type="protein sequence ID" value="ENSPNAP00000064828.1"/>
        <property type="gene ID" value="ENSPNAG00000032809.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159445"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000003">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000014">
        <property type="entry name" value="adenylate cyclase type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030672">
        <property type="entry name" value="Adcy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009398">
        <property type="entry name" value="Adcy_conserved_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF6">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06327">
        <property type="entry name" value="Adcy_cons_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039050">
        <property type="entry name" value="Ade_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="801"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="827"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="881"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="927"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="952"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="995"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="20">
        <location>
            <begin position="497"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="20">
        <location>
            <begin position="1078"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="73"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="745"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="95"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1275" mass="141539" checksum="C080F3344E5FD206" modified="2024-11-27" version="1">MQDDSHNCLEMVGVSNSEEPMEPRKQWHGDGYFNEGFTESLELTNTKNSEDFTSKEEDVGEIIKCSNHKDTQIDKDSIKTENNQTQRDAQIMHSDGQSTLKDADTTPSTDNSTCNVKDSLPSSNKTEHSSTVSQTCSRGMHDAKHLNIALGTAKQESIPEPLQVCTSFKPSNTTQNSSVNTLSQSTETHNLGESTLNGLGIPYSARSESNGECRTTSLEKAEGSRTQRSVAEGMCCCYQAVRQAFLRCLEETSIVVPGLVLTILFCVTIIIIIPTTGRSISVHVGALSVVCIVLCLCVPVLVSLPCLPALRRCEKLLALSIWIALFITAIVFIFTGGAITAWEQVAFFLFLSLSVYTILPLSLSWALIFGIGASVCHIVIISVYVSVTSPGTRDLAVQLVANAVLFVCVNCVGFFHLWLTEHARRKSNKSRQNFSSNCSKRAKLKRDQEHLLLSVLPRYIAMELKGEVVKGLSETFTGAENTPNFHSLYIKQHKDVSILYADIVGFTSLASTCTPEELVAVLNKLFGRFDDIAKKNECLRIKILGDCYYCVSGLPDSIPHHARNCVQMGLDMCNAINKLREATGVNISMRVGVHSGNVLCGVIGSLKWQYDVWSHDVTLANRMESGGLPGRVHITEETLGHLGGAYQVEEGNGASRDSALQGRRTFLVIDPHNRADKPKSNFLSSGREKLRASVRMSQYMQSWQSVKPFAELNQPEDCIPAMPHQSNGISQSHVFMELEIHQSHPGNLESRPTRVSENGSVDTLDPLQVTGQKPKKLNWLTLLFHDIKMEREYRLSEVHDIHQSVACLALIFTSVFVVQVLTSQKNLALAVSYGSTFPVQLLILLVVSTGFLKQWNSKIPTSVHWVSNLSQGVATRAALRLLLVFTCLLITLLMAILNIVFIPGDNCLNFSYNATALDSLNLFTVPYYLYCCLLAMMGVVVFVRVCFSVKLFLLTLAVVVYLALFLCVYAPRSDCLVQQLYPNYSKPGVLKEPKIMAGIWLIIFYLTSLILARQDELACRFEFLLKKYFQKEREDMETTDNVNRLLLQNLLPIHVTNFFIGKDVRNQDLYSQSCQSVCVMFASVPEFKDFYTESSVNGDGLECLRFLNEIITDFDELLSKPKFNVIEKIKTIGTTYMAAAGLTNPNTGEERKDSDACKSHVRSMVDFAIALMGRLDNINRHSFNNFKLRIGINHGPVIAGVIGAHKPQYDIWGNAVNVASRMDSTGVLDKIQVTEETAQVAQSVGYDVTKRGVINVKGKGELTTYFINIELSQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KN64</accession>
    <name>A0AAR2KN64_PYGNA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KN64"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079283.1">
        <property type="protein sequence ID" value="ENSPNAP00000063541.1"/>
        <property type="gene ID" value="ENSPNAG00000030939.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000268666"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="76" mass="8734" checksum="E04CB94EC470D6A0" modified="2024-11-27" version="1">FRKVLKKIRYIKYANLHSSARKLCMERTWTFQHDNDPKHKAKSTCHCGHLCLLTSVSLSHSGEISSTQFMQDSPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNB2</accession>
    <name>A0AAR2KNB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FXYD domain-containing ion transport regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FXYD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNB2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042837.1">
        <property type="protein sequence ID" value="ENSPNAP00000065823.1"/>
        <property type="gene ID" value="ENSPNAG00000030693.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287541"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099106">
        <property type="term" value="F:ion channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043269">
        <property type="term" value="P:regulation of monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.780">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047297">
        <property type="entry name" value="FXYD_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000272">
        <property type="entry name" value="Ion-transport_regulator_FXYD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02038">
        <property type="entry name" value="ATP1G1_PLM_MAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01310">
        <property type="entry name" value="FXYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043098675" description="FXYD domain-containing ion transport regulator" evidence="7">
        <location>
            <begin position="23"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="69" mass="7711" checksum="0176BB5C75C6D824" modified="2024-11-27" version="1">MKGFTALVLLTVFSAFLSEAEANPFVYNYDRLRIGGIIFTILLVIGAIFVLFHDKCGKKKKDEEEAGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNF4</accession>
    <name>A0AAR2KNF4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNF4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059772.1">
        <property type="protein sequence ID" value="ENSPNAP00000065868.1"/>
        <property type="gene ID" value="ENSPNAG00000031404.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162953"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001438">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF219">
        <property type="entry name" value="CARBOXYPEPTIDASE N SUBUNIT 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="624"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="11">
        <location>
            <begin position="412"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="481"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="550"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="689" mass="76284" checksum="55FEF87D89A31A6B" modified="2024-11-27" version="1">MILTSEVLYVISPFPVMGGNMLMGAGLYFTVLLHLLCCCAAEEPVVEPYQDVYDKDVTTIPAILKDGSKEVFFINSQIGVIPKGAFSKNPQLEKVEFIGTPTFSIEEGAFEGLEKLAIIEISGTNLTSLPIGVFKDLNNLNTLILKGNKIQNLERGLFDGISTLEKLQLHMNEISSIEEGTFDHLENLQELHLAKNNLAAVSTSLFSNLKKLQVFRIYDNKVTSMPDGIFDNLHNLKEISLYGNRITHIQPNLIPHKSKLIKLWLDNNLLTELPPNLFVDFTALKTLTLHHNQLTSLPSDLFGEMPKITELGLNNNNLTSLPARVFSPLKKLKKLDLSSNHFSHLSGEFFEGLDKLSELNLQKNTIEALKSDDFIQLKSLSTLNLAKNNLRSLPEDVFDSLPKLSKVYLKDNPWHCDCNLLSFYTWMKDNKDKLRPPSPDDARCQYPVDLRGQTILSLTTDQLICPTTLLTTTTLLTTTTPTTTVPTSLPTTTTSLPTTTTTLPTTTTLPTTTTEATTITITLQITTQPPTTSLPETTLLKTTATPSMSTPPILTTATTTTVPTPTATTTLTNTTVMTTIPISVEMSTVPSPTDLTTALTTPVTTTVQLTTPMNTAQTKTHRILLYKILMISCITMLLLVVCYALLLIKFMYSSPYNSPEEKERVILTHFSYKKSVSLRPVQDIESVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KRF7</accession>
    <name>A0AAR2KRF7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KRF7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043768.1">
        <property type="protein sequence ID" value="ENSPNAP00000065079.1"/>
        <property type="gene ID" value="ENSPNAG00000030767.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164735"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="287" mass="32578" checksum="8DEA372C0D5E6FBD" modified="2024-11-27" version="1">SFLRSLTTNLEFGSYSGYKLNFSKSTFYPINAAACTITQTEIPFRLAPDGFKYLGVNITRKISLIQDANFSPLLTKMCSDLQRWSSLPLSLAGRVQCIKMNVLPKFSFLFQCLPIFLPKSFFVSIDKAVTGFIWGGRVPRIRKGILQCPRSSGGLALPNFMYYYFAANIQKMAFWFKNPEAAWCILESLSCHSSSIRALVYSSLPILSSHFSSNPLVVSTLKIWTQFRRMFHFTEASVLSPICKNHLFEPATLDSRFISWERSGITTFKDLFDETFCSFSSYTCPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KXE1</accession>
    <name>A0AAR2KXE1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KXE1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042700.1">
        <property type="protein sequence ID" value="ENSPNAP00000068935.1"/>
        <property type="gene ID" value="ENSPNAG00000034856.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286900"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055247">
        <property type="entry name" value="InsJ-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13518">
        <property type="entry name" value="HTH_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Insertion element IS150 protein InsJ-like helix-turn-helix" evidence="2">
        <location>
            <begin position="32"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="88"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="32438" checksum="52CFBA06EA028DDE" modified="2024-11-27" version="1">MIGYKKSFSECQCLSEAKMVRGSPIPPLLRSNIVQQYQNGVTQRKIAKTFKLSSSTVHNIIKRFRESGTIAVRKGQGRKTLLDAPDLRALKRHCTSNRNATVKEITEWAQEYFQKALSVNTIHRAIHHCQLKLYSAKRKPFLSKLHKLRRLHWARGHLKWSVAKWKTVLWSDESRFKVLYGTLGRHVIRTTEDKDNPSYQRSVQNPASLMVWGCMSACGMGSLHVWKGTINAENYVQVLEQHMLPLDVISFREDPAFFNKIKPDHILQQSQHHGYVGEGSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KZ47</accession>
    <name>A0AAR2KZ47_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Apolipoprotein A-Ib</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein A1/A4/E family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KZ47"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041427.1">
        <property type="protein sequence ID" value="ENSPNAP00000069623.1"/>
        <property type="gene ID" value="ENSPNAG00000000734.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182929"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042627">
        <property type="term" value="C:chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034362">
        <property type="term" value="C:low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060228">
        <property type="term" value="F:phosphatidylcholine-sterol O-acyltransferase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055090">
        <property type="term" value="P:acylglycerol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033700">
        <property type="term" value="P:phospholipid efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000074">
        <property type="entry name" value="ApoA_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050163">
        <property type="entry name" value="Apolipoprotein_A1/A4/E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18976">
        <property type="entry name" value="APOLIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18976:SF11">
        <property type="entry name" value="APOLIPOPROTEIN A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01442">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0345">HDL</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="156"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="313" mass="35661" checksum="283147E0B4483C78" modified="2024-11-27" version="1">MPLIEKSDADLLSLHRSTMKFVALALAILLAAGKQCFSASTSSIQSQQLKPGSLVCTASKFKPPICPGCQARSMQADAPTQYEQIRSAVVTYLGQVKETAQKAIDRLDDAEYKEVKDRLTLSLTRIETFLESASESLKPVRETFGPQLLEVFAGVREKVEKDVEELRTELAPKREALREVVVKHIDEYREKLEPLLKEYTEKNREQMEYFRGKFEPVLKELRELIKTNVEETKSKLTPIVEVLVKKGRERLEEVKQALDPYIQEYREQAGSTLTSLREQYDSGELHKKISGLIEEVKPQLEKIAESVQKAFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KZM2</accession>
    <name>A0AAR2KZM2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KZM2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075278.1">
        <property type="protein sequence ID" value="ENSPNAP00000069803.1"/>
        <property type="gene ID" value="ENSPNAG00000032254.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214408"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="466" mass="52320" checksum="7060B85CA1E662D8" modified="2024-11-27" version="1">MELVCIDFWTAELSDKKTIDVLVVTDHFSKMSHAFPCRNQSAKQVARRLWNDFFCVYGFPKRIHSDQGANFESRLIKDLLEMAGVHKSHTTPYHPMGNGITERFNRTLGSMIRALPAKCKSKWPQMLQTLTFCYNCTVHETTGFAPFFLMFGRVPRLPIDVMFQHVLENDRVVSHHEFVQHLRRDLSEAAQIARKHALGEQDRHAKLYNRKIKGSPLAVGDRVLLANRGEKGKKKISDKWDSTPFDVVSVRSKINVYRIKDIYTGREKVVHRNLLLPVDFLIPDEQNGSQFSSVVSQSDDGVSDTVLVRSKEDSHARTMVWLMQSPENDQLDNDNTPDGCVDGSFDDLGHDLLPGEVSTDSHKDSANQNGTQSDNGTSQGPVHSTHLGVTHSVACSEPMGTVRDRSGGVVCTQPQSSRAISVSSRFGRTIRPTQRLICEMSDQRIVDNASPVMGSVLGFFRRALVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L176</accession>
    <name>A0AAR2L176_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PIK3C2G</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L176"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071990.1">
        <property type="protein sequence ID" value="ENSPNAP00000068382.1"/>
        <property type="gene ID" value="ENSPNAG00000031479.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163630"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="163"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="915" mass="105154" checksum="22DF54BDC69AA65F" modified="2024-11-27" version="1">MSKLRKLKLELNEIIDKKTQFLLQRLRWQNFEHSNKSGKFLANQLKLNKEKTTISSIKVSTGNITHDPQQINKAFKEFYKALYSSQINPSNAAIEEFLNNINLPKLKNEQAVALDSPLALPELHEALQHLPNNKAPGPDGFPVEFYKEFWSVLEPTFFRMVTQIQNENVLSPDMNSANISLLLKPGKDPTLPSSYRPISLINVDLKIICKVLARRLEEITPLIVHPDQTGFIKGRQSANNTRRLVNIIDYCTINKLETTIVSLDAEKAFDRVNWKYLLAVLHKFGFGSSFINWIKTLHSSPSARVRTNDITSQSFTLQRGTRQGCPLSPSLFVIFIEPLAAAIRQNTNIKGINTETTNHKISLYADDVLLFLENSQASLPNTIRLIDSFSSISDYSINWGKSTVLPINCSFQNTTAIPLQSGNIRYLGIHVSARLSDLVQLNHIPLLKTIEDDLKRWNALPISLMGRVATIKMMVLPKINYLFSLIPNKPPAAWFKSLDSNISQFLWKNKPSRISLKTLQKPKNNGGLELPNFYNYFLANRLQYISKWIKPDLLDSPWLDLEQAFCRKTKLSDLPYISSNIKRHDAFKSLSINSSLTAWWEFLKLTQSPLIPCKLTPIWNNPDICQNKKVLNFTSWREKGIKNLEHVIKEGNFITFQELISEYGINNSKFLEYQQLKSIIQERFNPNQLNLEISTWVTEFLNLCTPKLLSKLYKLLIKFDDSISLPIAKWERDLSINQDQSFWIEICSNIFKISKSPNLQLIQYKVLHRIHYTGQRMFQMGLAQSNICTHCTANVTDNYLHAIWDCRPVQMFWHRICTDLSTWLKCCIPTSPRLCILGDVSELVMDANTSHIVLTALCIAKKTILINWKQKKDLHITLYKNLLFDHISLERMSASSKNQLEEFNSLWLPLINSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L1Q7</accession>
    <name>A0AAR2L1Q7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L1Q7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044377.1">
        <property type="protein sequence ID" value="ENSPNAP00000068321.1"/>
        <property type="gene ID" value="ENSPNAG00000013832.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286919"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070051">
        <property type="term" value="F:fibrinogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000036">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000075">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040622">
        <property type="entry name" value="EGF_integrin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF25">
        <property type="entry name" value="INTEGRIN BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18372">
        <property type="entry name" value="I-EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002512">
        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 18" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0401">Integrin</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043400616" description="Integrin beta" evidence="20">
        <location>
            <begin position="19"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="739"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="21">
        <location>
            <begin position="42"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="23">
        <location>
            <begin position="644"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="22">
        <location>
            <begin position="762"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="35"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="46"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="210"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="266"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="408"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="467"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="490"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="534"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="536"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="541"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="556"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="578"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="580"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="596"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="617"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="619"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="624"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="637"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="644"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="671"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR002512-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="816" mass="89263" checksum="C715EFBB39D7066D" modified="2024-11-27" version="1">MCVCVCVCVCVCVCVCVCVCVCVCVCVFLGSSNICTSRGVRTCKECLSIHPTCAWCSKQDIGKGGPGLSRCNLKSILVQSGCGAEFIESPVSSMTVKEDRPLSDKAQGSTRDITQIQPQRIHLTLRPGDSKRFTVTVRQVADYPVDLYYLMDLSYSMKDDLVSLRSLGNELATAMGQATSNLRMGFGAFVDKTLSPYMYMFPDEAVKNPCVGLPATVCRPQFGYKNVLSLTEQVSRFTEEVKNQEVSRNRDAPEGGFDAVLQAVVCKDKIGWRSDASHLLVFTTDANTHTALDGRVAGIVQPSDGQCYVNNENNYDKTTLLDYPSIAMLADKLSENNINLIFAVTDNVMPLYLNYSKLIPGTTVGRLSYDSKNIIQLIQDAYTNIRSKAELEVLGVPDELTLSFNATCLNGEVVPGVRSCLGLKIGDTVSFNVEATLRACPKEKNRTFNIKPMGFKDTLQVTVDYACNCACHDSAQPASPACYGNGTLECGVCLCDAGRLGPHCECTEEDHKPTEADSCSPEPQAPICSGRGDCLCGQCSCHSNNFGKVWGKYCECDDFNCIRSKGQLCSGEACHGDCVCGFCQCNPDWLGESCNCSTRTDNCMGSIGLLCSGRGHCTCGVCECTQPGAYGATCEKCPTCPDSCTIKKDCVECKHYKRGTLFEKNSCNTICRDDITLVDELGNTYFHTSLFSHVSDDCTVYIGTPCKHEQTSLSLSLSRSLSLSRTLCVCVECPMGPDILVVMAYVAGSILLLGLAALLIWKLLVTIHDRREYAKFEEERAKAKMLFTYKRSVHKCFNIKGIIWQKKYIVFPLVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L2P2</accession>
    <name>A0AAR2L2P2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nucleoporin 160</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NUP160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000081336.1">
        <property type="protein sequence ID" value="ENSPNAP00000068641.1"/>
        <property type="gene ID" value="ENSPNAG00000017050.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000972"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021717">
        <property type="entry name" value="Nucleoporin_Nup160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056547">
        <property type="entry name" value="NUP160_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056536">
        <property type="entry name" value="TPR_NUP160_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056535">
        <property type="entry name" value="TPR_NUP160_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23345">
        <property type="entry name" value="NUP160_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23354">
        <property type="entry name" value="TPR_NUP160_120_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23347">
        <property type="entry name" value="TPR_Nup160_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="4">
        <location>
            <begin position="81"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 helical" evidence="5">
        <location>
            <begin position="571"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 middle TPR" evidence="7">
        <location>
            <begin position="846"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 C-terminal TPR" evidence="6">
        <location>
            <begin position="1154"/>
            <end position="1399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1403" mass="156501" checksum="3D72069A860127BE" modified="2024-11-27" version="1">MSSVLIGCASFSNINTPYNFSISRRGSNFSLMNIHIFEPVDQVLCLCAGVSAPPGGVKLPDSAGGFSYEESGTLLSITSNRFIHWSTSGDTVQLVEQSLDTNLLNNAIRLKFLHCSVLPGGVHIHETLNNVIILIVTNLSVHRLVLPHPTRMYRSELVTELQMQSVFTDVGKLSIQDEAHSFILPQSPAASAAWISPNGHAHFALAPPTGGVTVVTLPPHDQQGSVSLLELKQSSVMQRLAGWMPSAIRGEQSPCDLAVSLAAWSLEDDTFIFALCQDHKLRVWSFKELQCVLVADLLDYVPVGRAELRCVPGQAHRLRVCFSGSTGLCVCVYLAIPKRGQFCVLQLMASDNGRYSLENISTQFSTQETLVDFALTSTDIWGLWVDDDNQTVVKYINFEHNTAGMWNQVFVQPPPEEEIHIGLDQDPRETYLDVLFSPLRFTAEAIVKALQIYKRGTERFVDVPWDALKKEVTLAVENELQSSVTEFEFSQEDYRMLQVEFWSKFYACCLQYQEALSTPLALHINQATAMVCLLKKGFVSFLLPCFAVDHLYLSADEYLSSEEETPVSEGDSDLSRDVLQMVQCLRMVSDCVQGEMACEMEKALELLLSPERAAEQVLENMLANDSENVIEDITNKLQDVRNPLAAMGALLRELDLETDMETGPETTAPPAGQSLNVRISLSQLYGSTVAVSVVCQAVCQVALTRALLCRDLLILQHLYLRLGDNVSGAQSLQLQQDLIPRCSHLLCCYYLLKQLSQTLASPVPLDTLDANLQHLSVLELSDSAALAPNRSVLNPQTVVELFYQNVGRKVIVSQLFGQRDLQAAQSPLHWPQLSSSVVTLLGQLLWPSNPGFLFPECMMGNCQYTQLQEYVRLIGPWCQANVGSCRFILGQAYLASGEGQKALQCFQEAAPEVEKEEFLNRLTGSEEEAGTTAPRLLYYNKVLRLLEDVGLPELVIQLATLAISEAVNDERSLAALWTRIFKHHLDLGHNREAYEALTQNPDASRQLDCLRQLVVVLCERAQLQDLVQFPYVNLHEEVVSIIESRARAVDLMTHNYYELLYAFHINRHNYRKAGTVMFEYGMRLGREVRTLRGLQKQVNCFLAALNCLRLIRPEYAWIVQPSPGPAVITPPVHAVNGVCDAVVSAVSRQIEILELQDLEKEYILSRCRLTLAQQHPPSAAIAGSASAAEMVALLVQAGLFDTALSLCQTFKLPLTPVFEGLTFKCIKLQYGGEQSQNEAWNWLAANQLPSLITTKECSATDEAWRLLASYLEKHQSQNAQYYRCVINRLLSHGVPVPDWLVNAYKKVDAAALLRLYLNYDQLESAAELVLEYVDALLGKGHQYFGIEMPLSAMAPLVWLPYTSIDQLLHSLRDSQANTQKLSVKLAEYHRVVEQSTRHRLHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L489</accession>
    <name>A0AAR2L489_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="8">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RING-type E3 ubiquitin transferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017561004.1">
        <property type="nucleotide sequence ID" value="XM_017705515.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L489"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050918.1">
        <property type="protein sequence ID" value="ENSPNAP00000071418.1"/>
        <property type="gene ID" value="ENSPNAG00000001718.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431992"/>
    <dbReference type="CTD" id="793939"/>
    <dbReference type="GeneTree" id="ENSGT00390000009925"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043951">
        <property type="term" value="P:negative regulation of cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045744">
        <property type="term" value="P:negative regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000013">
        <property type="entry name" value="E3 ubiquitin-protein ligase MGRN1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045194">
        <property type="entry name" value="MGRN1/RNF157-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058981">
        <property type="entry name" value="MGRN1/RNF157-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MGRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22996">
        <property type="entry name" value="MAHOGUNIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26192">
        <property type="entry name" value="RNF157-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="410"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="557" mass="61549" checksum="52B1085EF126084A" modified="2024-11-27" version="1">MGSILSRRIAGVEDIDIQANSAYRYPPKSGNYFTSHFFMGGEKFDTPHPEGYLFGENMDLNFLGNRPVQFPYVTPAPHEPVKTLRSLVNIRKDSLRLVRYKDDAEGMTEETENPRVLYGVEFCFDTDARVAITLYCQAFEEFSNGMAVYTPKTPALVSETVHYKREINQQFSLPSFKIDFTKWKPEELNFDLDRGVFPLVIQAVVDEGDDVIGHAHILLAAFEKHVDGSFSVKPLKQKQIVDRVSYLLQEIYGIENKNNQKTKSPEDENSDNSSECVVCLSDLRDTLILPCRHLCLCNACADTLRYQANNCPICRLPFRALLQIRAVRKKPASMLSSVSFSPVLSQSSDHTENTNSENVPPGYEPVSLLEALNGVQALPPAIPSAPLYEEIQFTGEVGDPLNLSRRQQSMLEHSPDPAALKGKGSKSPDGSIRSPSSPIQEEEDEERLSELSDAQPRTVLPYSPAPTEGTIDGEISEPASPNTESRTLGVSVSEILQDCHSARSSLSRTESDPSADLALPGSSGSIESLKSQSTSSSSQPLLCLPSSLHMEDERLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L9Y1</accession>
    <name>A0AAR2L9Y1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L9Y1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053980.1">
        <property type="protein sequence ID" value="ENSPNAP00000071457.1"/>
        <property type="gene ID" value="ENSPNAG00000033356.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235350"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="102" mass="11590" checksum="3ADB8787E69168B8" modified="2024-11-27" version="1">MTAGAQRRMLNEVKKNPRVSAKDLQKSLAHGTFFDDKSTISKTLNKNGVHGRTPRRKPLLSKKNIDARLKFAKEHLDVPQCYSCQIFTYSSTLHCECSHCVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBC8</accession>
    <name>A0AAR2LBC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBC8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048622.1">
        <property type="protein sequence ID" value="ENSPNAP00000073898.1"/>
        <property type="gene ID" value="ENSPNAG00000033499.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178018"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="2">
        <location>
            <begin position="67"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="218" mass="25434" checksum="037C5C9E7B238F96" modified="2024-11-27" version="1">QLSEEIFSRVLYYVQRRRKPTARERGTESACVLRLLRHWKKLKIRNGILYRVKRHHRLNRKVFQFVIPGSLKKQVLHGVHDAAGHQGCARTLSLVSERFFWTGMNTDVMYYVKHCKRCILGKTPEPDARAPLENIHTSEPMELVCIDFWTAELSDKKTIDVLVVTDHFSKMSHAFPCRNQSAKQVARRLWNDFFLEWLHAAVVSTALYVQLSKTAKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LDI9</accession>
    <name>A0AAR2LDI9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Microtubule associated tumor suppressor 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MTUS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037388727.1">
        <property type="nucleotide sequence ID" value="XM_037532830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LDI9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070074.1">
        <property type="protein sequence ID" value="ENSPNAP00000072774.1"/>
        <property type="gene ID" value="ENSPNAG00000032493.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411403"/>
    <dbReference type="CTD" id="557250"/>
    <dbReference type="GeneTree" id="ENSGT00950000183026"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051293">
        <property type="entry name" value="MTUS1/CCDC69"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24200:SF7">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED TUMOR SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24200">
        <property type="entry name" value="TOUCAN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="412"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="586"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1142"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="849"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="936"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1070"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="479"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="586"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="724"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="750"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="786"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1146"/>
            <end position="1161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1174" mass="129617" checksum="3FC7A5A637633E53" modified="2024-11-27" version="1">MSGDMSEPQPFALTMKPSEGVHHGMCLPLHSGAVDANAFAESSSMSSASPDSMRSLSSLSGARTDSPLDVEMPEGRMGRKVTTGEDDSGIQSPDCRAENDNDNSVSVYLDANEEGWCEIHDGQDNVTLVLTLKQGQCDDRSDGHSNGDRGRRSSDDSSATEAVLCCSGYEEEDDEEEDSFLSLSSVDVVMRCQNEEGHIQISSMGPNVDPVSELHKEALQESVEKDQMQSSSRRPSGDSLSEFHQAGLQELTESHTEEGQMHNSSRRPDVGLVNELHQKTLHESMEIDSVECEVHHSIRKPHMGHESELDQEDLQESMEIHTEEFQMHSSTRMPDIGHVNELHQEALQESTQLDTEEGQMQSSSKRPNVGPNCEFHQEALQDFVGPHVELNLSHLPSALPVVSEDNFVASAPTQEPKKTVSSITHHKNDKTKLTLQNTKDRPGQGHTRPTTKPSMTKSVKTEIKRFPRPDLKNVKPKINSRAASAPRPANPSKSTAGTEKKASPPRGRTANSKDQDADGVIKKRRSSSNQARVTMPAPITDPKPKATHLQTERAPPCVSDASVSPEIVVNGDQEQNKGAEVMDIVNEERECDKEKVPLETQSAEETPEDLDQTGSELKTPEAEGGNLPTGVRAPGPAGDPKTCSPASSCRAPQKPASSKLPIKSRGLTASLSSSSLGSAASESNTPGGRGVTQVVKSEEKSVRSSSTSQVKPANNRPAVGPRNRANSTPAKPTTAGQKAPALFTIAAVKPTQNALQRSGSARPNRPVGTTVEKSSRGVRSVPGRPHGSGGGGGGGGGVVTHTEDEEEKQLRLQNERKNQCILQLRKLVAHGNRRLEALTLVIQHIFNEREEALKQKKDISFQLKNLREELANSVSCCERLQKEKEEVCVALEAAVLKLQQQHQDELTQLEETLRDFYSAEWDKTHQAYQEEADKCRAFMQQQVEEVRSKQEALRQEQEAAHAQEMEALKEEYETTLTDFRKAHKNNMQELESMREQSEASMNERIASLMEENEALSAKLKEEEERRKALADKSQKDPHMVYLEQELESLKVVLDIKTSQLHQQDKKLMQMDKLMESNVKLEECLKKLQQENEDYKARMDKHAALSRQLSTEQAALQQTLQMESKVNKRLSMENEELLWKLHNGDLSSPRRLSPSSPFHSPRNSASFPSAPISPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LEY1</accession>
    <name>A0AAR2LEY1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552324.1">
        <property type="nucleotide sequence ID" value="XM_017696835.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LEY1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079653.1">
        <property type="protein sequence ID" value="ENSPNAP00000075075.1"/>
        <property type="gene ID" value="ENSPNAG00000037649.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426975"/>
    <dbReference type="CTD" id="406465"/>
    <dbReference type="GeneTree" id="ENSGT00730000111086"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061513">
        <property type="term" value="F:glucose 6-phosphate:phosphate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17343">
        <property type="entry name" value="MFS_SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000112">
        <property type="entry name" value="glucose-6-phosphate exchanger SLC37A4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000111">
        <property type="entry name" value="Solute carrier family 37 member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051337">
        <property type="entry name" value="OPA_Antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021159">
        <property type="entry name" value="Sugar-P_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00881">
        <property type="entry name" value="2A0104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826:SF11">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE TRANSLOCASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00942">
        <property type="entry name" value="GLPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="13"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="429" mass="46661" checksum="01400E43340F3075" modified="2024-11-27" version="1">MVKSGYGYYRTTIFLAMFVGYTLYYFNRKTFSFVMPSLMQEIKLDKDDLGLITSSQSLAYAISKFISGVLSDQISARWLFSIGLFMVGGINVLFSWSSTVAVFSALWFLNGLGQGLGWPPCGKVLRKWFEPSQFGTWWSVLSCSMNLAGGLGPIIATLLAQSYSWRSTLSTSGLICVVMSIFCLLVIHNEPSDVGLPNIEASAKKGKAGSSKDESTLKEFLLSPYLWLLSLGYLVVFGVKTTCTDWGQLFLIQDKGQSALMGSSYMSALEIGGLVGSLAAGYLSDRAVARQGLHVYGNPRHGLLLSMMAGMCISMYLFRTTITQESSNVWILSLGAAFGFSSYGPIALFGVIANESAPSNYCGTSHAIVALMANVGGFLSGLPFSTIAKHHGWDTAFWVAEVTCVVTTIGFFLFRNIQTKMGRAIKKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LG78</accession>
    <name>A0AAR2LG78_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LG78"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086819.1">
        <property type="protein sequence ID" value="ENSPNAP00000073729.1"/>
        <property type="gene ID" value="ENSPNAG00000036911.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="56" mass="6226" checksum="C471678C739240EA" modified="2024-11-27" version="1">MKFSKISWSAEALRVPFTGTKGPVDCGIFGSEEISRLDLLHRSCPVTTPESDPFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LHK9</accession>
    <name>A0AAR2LHK9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="18">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">B4GALT5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17950"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:31496"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Golgi apparatus membrane</location>
            <topology evidence="18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LHK9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042512.1">
        <property type="protein sequence ID" value="ENSPNAP00000076010.1"/>
        <property type="gene ID" value="ENSPNAG00000023303.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158138"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008489">
        <property type="term" value="F:UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009101">
        <property type="term" value="P:glycoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000037">
        <property type="entry name" value="Beta-1,4-galactosyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF45">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="116"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="256"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="391" mass="45374" checksum="43F7467C639C7F6B" modified="2024-11-27" version="1">MYFKATLLISSQLGILESLKSQVWNVIVHLNPLLLFSLFYKLAVLNESYLFMVQARGMRIRQNMRNIGAQVLEQVVRSAYNINGTDYPYDFNLSETAAPPTPYLPAGFTYLPTDICPERLPTMKGRLEVNMSEIALEEVERGLRQVDPSLGLGGHWKPQDCIPRWKVAILVPFRNRHEHLPILFRHLIPALQRQRLEFGFYVVEQASDEPFNRAMLFNVGFKEAMKDLHWDCVIFHDVDHILENDRNYYGCSEMPRHFAVKLNKYSYMLPYDEFFGGVSGLTSEQFQKINGFPNAFWGWGGEDDDLWNRVQYAGYTVSRPPGDIGRYMSIPHHHRGEVQFLGRYTLLRHSKERQRLDGLNNLRYSPQVSRRSLYTNISVALSRDLAPIADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIW4</accession>
    <name>A0AAR2LIW4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LIW4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041980.1">
        <property type="protein sequence ID" value="ENSPNAP00000076488.1"/>
        <property type="gene ID" value="ENSPNAG00000037109.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271821"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015095">
        <property type="entry name" value="AlkB_hom8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09004">
        <property type="entry name" value="ALKBH8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="290" mass="33293" checksum="3C0B68417D6A918D" modified="2024-11-27" version="1">MCNWVLEFLTDRPQSVRLGSHMSTTITLNTGVPQGCVLSPLLFSLFTHDCVPLHTSNILIKFADDTTVVGHIRNNDESAYREEIQHLTSWCTTNNLILNTGKTKELIVDYRKTKGCTHSPVYINGAEVERVSSFRFLGIHISADLSWSFNTSHLIKKAQQRLYFLRRLKQDRLSPQILTNFYRCTIESILTNCVTVWYSSSTVAERKSLQRVVRTAQRITGMQLPAIKDIHHQRCVRRAHSIIKDPSHPNHKLFNLLPSGRRYKSIRARTSRLRSSFFPSTITLLNSTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJ99</accession>
    <name>A0AAR2LJ99_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calpain-1 catalytic subunit</fullName>
            <ecNumber evidence="6">3.4.22.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Calpain-1 large subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Broad endopeptidase specificity.</text>
            <dbReference type="EC" id="3.4.22.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.52" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017550901.1">
        <property type="nucleotide sequence ID" value="XM_017695412.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJ99"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071868.1">
        <property type="protein sequence ID" value="ENSPNAP00000074747.1"/>
        <property type="gene ID" value="ENSPNAG00000017097.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426129"/>
    <dbReference type="CTD" id="393417"/>
    <dbReference type="GeneTree" id="ENSGT00940000158672"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000124">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF322">
        <property type="entry name" value="CALPAIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="14 19" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="20">
        <location>
            <begin position="48"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="606"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="active site" evidence="18 19">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" evidence="18 19">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="active site" evidence="18 19">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="704" mass="80564" checksum="87C65BE8AA85071A" modified="2024-11-27" version="1">MFSYGGISAHISASRLKAEGIGSCEQAVHFQNQAFEDLKRECLEGGFLFEDPCFPAEPPSLGFKELGPYSSKTHGVEWMRPTELCDDPQFIMGGATRTDICQGALGDCWLLAAIASLTLNEKILHRVVPHGQSFQEDYAGIFHFQFWQFGEWVDIVIDDRLPVKDGELMFVHSAEGNEFWSALIEKAYAKLNGSYEALSGGSTTEGFEDFTGGVSEMYELNKAPKDLYRIISKALERGSLLGCSIDITSRFDMEAVTFKKLVKGHAYSVTGLSQVNFRGHTERLIRIRNPWGQVEWTGAWSDNSPEWDEIDPSEREDMCLQMEDGEFWMSFSEFLRQFSRLEICNLTPDALSEDAMSHWNTTAFHGSWRRGSTAGGCRNHPNTFWINPQYKITLLEEDDDPEDDEVACSFLVALMQKDRRRHRRHGQDMHTIGFAIYEIPDEFRGCQNVHLKKNFFLTHSSCARSETFINLREVSTRLRLPPGEYLIVPSTFEPSKEADFVLRVFTEKQSETEELDDDISADLEEEEEISEDDIDDSFKSMFAQLAGEDMEISVHELRTILNRVVLRHKDLKTDGFSMESCRTMVNLMDKDGSAHLGLVEFQILWNKIRKWLVIYRQFDLDKSGTMSSYEMRLAVESAGFKLNNRLNQILVARYAENESIDFDNFICCLVKLEAMFRSFQQLDKEGTGDVEMNITEWLYITMCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLW2</accession>
    <name>A0AAR2LLW2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TLX1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017578327.1">
        <property type="nucleotide sequence ID" value="XM_017722838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLW2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057189.1">
        <property type="protein sequence ID" value="ENSPNAP00000077530.1"/>
        <property type="gene ID" value="ENSPNAG00000033485.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108442670"/>
    <dbReference type="CTD" id="3195"/>
    <dbReference type="GeneTree" id="ENSGT00940000158698"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000040">
        <property type="entry name" value="T-cell leukemia homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042247">
        <property type="entry name" value="TLX1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921">
        <property type="entry name" value="IP01054P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921:SF2">
        <property type="entry name" value="T-CELL LEUKEMIA HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="173"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="175"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="300" mass="33080" checksum="F5B2349D2C7DB9D8" modified="2024-11-27" version="1">MENIAAHLQHTHVDAISFGIDHILSNADPSSCMISNPRMQDLDCGLGGIVSTAYNTVTGQYGSGNGGYGGGGACGGSYAMNAGMNVNGNVSGLSSSGVIRVPAHRPLNGVHASIQTNGVTAPSSMSSMNNLTGLTFPWMESNRRYTKDRFTVALSPFTVTRRIGHPYQNRTPPKKKKPRTSFTRLQICELEKRFHRQKYLASAERAALAKALKMTDAQVKTWFQNRRTKWRRQTAEEREAERQQANRLLMQLQQEAFQKTLNQPVSPDPICLHNSSLFALHNLQPWTENTGKISSVSTCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LP64</accession>
    <name>A0AAR2LP64_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Pyruvate carboxylase</fullName>
            <ecNumber evidence="3 11">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="3 11"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LP64"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067974.1">
        <property type="protein sequence ID" value="ENSPNAP00000076457.1"/>
        <property type="gene ID" value="ENSPNAG00000011172.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000141164"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009374">
        <property type="term" value="F:biotin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07937">
        <property type="entry name" value="DRE_TIM_PC_TC_5S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000018">
        <property type="entry name" value="Biotin carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.600.10:FF:000001">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000033">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000012">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006761">
        <property type="entry name" value="PRK09282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009554">
        <property type="entry name" value="PRK12999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001594">
        <property type="entry name" value="Pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="18">
        <location>
            <begin position="44"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="164"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="19">
        <location>
            <begin position="571"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="16">
        <location>
            <begin position="1117"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="580"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="14">
        <location>
            <position position="749"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="779"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="781"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="916"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="749"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-biotinyllysine" evidence="15">
        <location>
            <position position="1152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1187" mass="131134" checksum="BC67243CB2A30B73" modified="2024-11-27" version="1">MSSKKRMLLYFGGVKNGLGLLALRRVYLLSRSAHTSPQTLEYRPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGKGLPPVTAYLHIPDIIKVAKENNVDAIHPGYGFLSERSDFAQACEEAGVRFIGPSPEVVRKMGDKVEARALAIQAGVPVVPGTDAPISSLQEAQEFAKTYGFPIIFKAAYGGGGRGMRVVRECEELEENYQRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDKYGNVIHLYERDCSIQRRHQKVVEIAPAAQLDPQLRDKLTSDSVNLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQLRVCEGRSLPELGLKQDKIKINGCAIQCRVTTEDPARGFQPDTGRIEVFRSAEGMGIRLDSASAFQGALISPHYDSLLVKVIASGKDLPTAASKMLRALAEFRIRGVKTNIPFLQNVLSNHQFLYSTVDTQFIDENQDLFNLKRAQNRAQKLLHYLGHVMVNGPTTPIPVKAKPSSTDPVIPSLPMGEPPAGFREVLLKEGPEGFARAVRQHQGLLLMDTTLRDAHQSLLATRVRTHDLKKIAPYVAHNFSNLFSLENWGGATFDVAMRFLYECPWRRLQELRALIPNVPFQMLLRGANAVGYTNYPDNAVFKFCEVAKENGMDIFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVSDPMRQKYSLEYYIQLAEELVKAGTHILSIKDMAGLLKPEPSRLLITALRDRFPDMPIHVHTHDTAGAGVAAMLACAEAGADIVDVAVDSMAGMTSQPSMGALVACTKGTKHDTGISLEKVFDYSEYWEVTRGLYAPFDCTATMKSGNADVYENEIPGGQYTNLHFQAHSMGLGNKFKEVKKAYAEANRLLGDLIKVTPSSKIVGDLAQFMVQNSLSRADVEERADELSFPLSVVEFLQGHIGIPHGGFPEPFRSKVLKSLPRVEGRPGASLPAMDFKKLEADLRMAHGSEITPEDVMSAAMYPKVFNEFKEFRRNFGPVDRLNTRLFLDGPKIAEEFEVELERGKTLHIKALALGDLNKAGQREVFFELNGQLRSVLVKDTVAMKEMHFHPKALKDVRGQVGAPMPGKVVELKVKQGQKVEKGQPLCVLSAMKMETVVNSPLSGTVAKVHVKADSSLEGDDLILEITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LTA7</accession>
    <name>A0AAR2LTA7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Paired domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MRPL47</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LTA7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076470.1">
        <property type="protein sequence ID" value="ENSPNAP00000077887.1"/>
        <property type="gene ID" value="ENSPNAG00000037872.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287007"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057667">
        <property type="entry name" value="HTH_SB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25787">
        <property type="entry name" value="HTH_SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sleeping Beauty transposase HTH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="70"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="146"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="39521" checksum="59DA1AF3D0DB17A3" modified="2024-11-27" version="1">MGKTKEHSIQVRQMCVDLHRSGNGYKKIATHLHLSVSTVRGIIKKFKTTGTVVNKSGRGRKFILPPRTVRRMIREIKSSPKLTVTELQQMVASWGHEVSKTTIRRYLHANKLFGRHARKKPFLTHNHKRKRLEFAKQYWDFNWDRVLWSDETKIELFGNKCCKWVWRNTRAEYAEKHLMPTVKYGGGSVMLWACFSSKGPGNLVRVHGIMNALKYQDILKQNLVSSARKLKMGRHWVFQQDNDPKHMARSTQKWLTTHRIKLLPWPSQSPDLNPIENLWGELKRRVQRRGPRSLDDLERLCKEEWSKIPLSVFSHLVKHYRRRLGAVLLAKGGCTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LWP4</accession>
    <name>A0AAR2LWP4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) kinase</fullName>
            <ecNumber evidence="2">2.7.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NADK</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LWP4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065470.1">
        <property type="protein sequence ID" value="ENSPNAP00000079164.1"/>
        <property type="gene ID" value="ENSPNAG00000019184.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013792"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000003">
        <property type="entry name" value="NAD kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF30">
        <property type="entry name" value="NAD(+) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="520" mass="57830" checksum="F7E06B2645CF77F9" modified="2024-11-27" version="1">MMVVDPHRLQPASQEVQDPITERGVQSKCLEFVSQGLGNHGVKGRCEIHKEHTDVRVLTLQVGEGSVDHRGDDILPGSFFPMENSEEDSWTPKSETCVESLPHCCSACDNRESLIPGKTFRKPKARSMSTSAVPSCAEFRRTQSLHGPSPVTTFGPKACMLKNPKAVMHIQDPASQRLTWNKPPKSVLVIKKIQDVSLLQPFKELCIFLTEQKNMIVYVERKVLEDPAIANDESFVAVKKNFCTFREDFDDLSNRVDFIICLGGDGTLLYASSLFQESVPPVMAFHLGSLGFLTPFNFDTYQSQVTQVIEGNAAIILRSRLKVKVVKESREKSKTKDKNLIITNGDDEPSRKTMQYQVLNEVVVDRGPSSYLSNVDLFLDGHLITTVQGDGVIVSTPTGSTAYAVAAGASMIHPNVPAILITPICPHSLSFRPIVVPAGVELKIMLSREARNNAWVSFDGRKRQEIGHGDSITITTSCFPVPSICFRDPVNDWFESLAQCLHWNVRKKQSHLSSEEEEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZW1</accession>
    <name>A0AAR2LZW1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">M-phase phosphoprotein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MPHOSPH6</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017555529.1">
        <property type="nucleotide sequence ID" value="XM_017700040.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LZW1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068456.1">
        <property type="protein sequence ID" value="ENSPNAP00000080202.1"/>
        <property type="gene ID" value="ENSPNAG00000032623.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108428769"/>
    <dbReference type="CTD" id="10200"/>
    <dbReference type="GeneTree" id="ENSGT00390000009212"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019324">
        <property type="entry name" value="MPP6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13582">
        <property type="entry name" value="M-PHASE PHOSPHOPROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13582:SF0">
        <property type="entry name" value="M-PHASE PHOSPHOPROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10175">
        <property type="entry name" value="MPP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19822" checksum="D513E397446EE303" modified="2024-11-27" version="1">MANDGSTKLSKNLLRMKFMQRGLDAEVKKQLEEEEKRIISDEHWYLDLPELKAKECYIVEERSYIPCEDLVYGRMSFKGFNPDVEKLMVLMNVPKEREKDEEEDDMTRMDTDITDEDMARRYGSLVESMKRRFAKKRERSSLQDSLEDVNRNVVPSQPKKTFLKPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M1B6</accession>
    <name>A0AAR2M1B6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17 25">Postsynaptic cell membrane</location>
            <topology evidence="17 25">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000066226.1">
        <property type="protein sequence ID" value="ENSPNAP00000080702.1"/>
        <property type="gene ID" value="ENSPNAG00000006351.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155964"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017146">
        <property type="term" value="C:NMDA selective glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004972">
        <property type="term" value="F:NMDA glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06378">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13718">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000199">
        <property type="entry name" value="Glutamate receptor ionotropic, NMDA 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000020">
        <property type="entry name" value="Glutamate receptor ionotropic, NMDA 2B, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000007">
        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000038">
        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018884">
        <property type="entry name" value="NMDAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10565">
        <property type="entry name" value="NMDAR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 25" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 25" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 25" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 25" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 25" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 25" id="KW-0732">Signal</keyword>
    <keyword evidence="8 25" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 25" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043099585" description="Glutamate receptor" evidence="25">
        <location>
            <begin position="25"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="582"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="654"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="845"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="28">
        <location>
            <begin position="466"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="27">
        <location>
            <begin position="470"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1194"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1299"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1375"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1645"/>
            <end position="1676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="1194"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="26">
        <location>
            <begin position="1303"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="538"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="714"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="715"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="756"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="23">
        <location>
            <position position="687"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="23">
        <location>
            <position position="720"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="770"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00038189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1676" mass="181337" checksum="6884AA39D6F40496" modified="2024-11-27" version="1">MLVSLRLSLLVLPSLLLFLPACESRRGGGGGSTAGGVGAGGVSSSTRGHSIIGPPHYPPAAVNPVAAPLPPKFAQGLSIAVILVGNSSEVTLAEGLEKEDFLHVPLPPKVDLVTMNETDPKSIINRICALMSRNWLQGVVFGDDTDQEAIAQILDFISAQTHIPILGIRGGSSMIMAAKDDHSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYYPGYQDFVNRVRKITIENSFVGWELEEVLLLDMSVDDGDSKIQNQMKKLQSPVILLYCTKEEATTIFEVAHSVGLTGYGYTWIVPALVAGDADNVPPVFPTGLISVSYDEWDYGLEARVRDAVAIIAMATSTMMLDRGPHTLYLMNVTFEGRNLSFSEDGYQMHPKLVIILLDKERQWDRVGKWENGSLTMKYHVWPRFELYSDAEEREDDHLSIVTLEEAPFVIVEDVDPLSGTCMRNTVPCRKQLKAQNQTGDSGIYIKRCCKGFCIDILKKIAKTVKFTYDLYLVTNGKHGKKVNGTWNGMVGEVVAKNAHMAVGSLTINEERSEVIDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYKEMHSYMTSFHQKNVDEALHSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGYIFATTGYGIAIQKDSGWKRPVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDVDNMAGVFYMLGAAMALSLITFIAEHLFYWQLRFCFMGVCSGKPGITFSISRGIYSCIHGVQIEENKSGLNSPSATMNMNMNNTHSNILRLLRTAKNMTSVPGVNGSPHSALDYGHRESAVYDISEHRRSLVGHSDCKPPPPYLPEDNMFSDYVSDVERTFGNLHLKDSNLYQDHYLHHHGGTGSGLALGMSGPLPNRPRSLGSASSLEGGMYDCDSLGGGVAPIFTTQPRQSLTHRNREKFDLIAGHPTQSSFKSGLPDLYGKFSFKGGASSSGFIAGHDRYCGGGGAGSGGDDGNIRSDVSDISTHTVTYGNLEGNAKRRKQYWDSLKKRPASAKSRREQDEIELGFRRRTHHTIHHHHHHHPVSQGHRSATPPVERKSQRGGNCSSYLFRDKENLRDFYKEGASPWEHLDLSDAPGIGGGGGLGGGGCGGIVGSGGGGGACTSLVPVDDFLKGKSKKTEVKGGVGGGGGGGGSPGQQGHACWEKNIGGVGGLTGGDWECRNCHSGGGGGGKPVCMHGGGGGGGYAAAGGVGGSSGGGGGGSGQVSSRPSSATCKRCDSCKKPGNLYDISEDNHLLLDQIGGKFLLEGGKGGGGAQTQAQRRKFGPGGRVLRRQHSYDTFVDLQKEGAASMLPPPRSVSLKDKDRYMEGASPYAQMFERYAGGEREPSFFAGDRGKGGGSSFSLFRVGTYSLSKSLYPDKVNQNPFIPTFGDDQCLLHGAKSYYIKKQQTQPQQQQTQQQQQQLLNNNRADFRGSMGVTSYLPVSASAGVMSNVGPRFPKELCLGGPLSNHHGGAAGNNKLLSARDGLGMGQGQRPFNGSSNGHVYEKLSSIESDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M5V3</accession>
    <name>A0AAR2M5V3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M5V3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083746.1">
        <property type="protein sequence ID" value="ENSPNAP00000082702.1"/>
        <property type="gene ID" value="ENSPNAG00000033670.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00740000115534"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000012">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043669629" description="UBC core domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="22"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="157" mass="17923" checksum="4E9F56BFB5403154" modified="2024-11-27" version="1">MQVFCLLLGVLCGFVCPGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWRGMIIGPPRTIYENRMYSLRVECGPRYPETPPFVRFVTKINLNGVHNSNGVVDTRAVSSLAKWQNSYSIRVVLQELRRLMMCKENMKLPQPPEGQIYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M5X3</accession>
    <name>A0AAR2M5X3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA helicase</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">DEAH box protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Nuclear DNA helicase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">DHX9</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017572568.1">
        <property type="nucleotide sequence ID" value="XM_017717079.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M5X3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073402.1">
        <property type="protein sequence ID" value="ENSPNAP00000082441.1"/>
        <property type="gene ID" value="ENSPNAG00000021343.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438944"/>
    <dbReference type="CTD" id="1660"/>
    <dbReference type="GeneTree" id="ENSGT00940000155924"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033679">
        <property type="term" value="F:3'-5' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="14" id="KW-0347">Helicase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="25" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="23" id="KW-0804">Transcription</keyword>
    <keyword evidence="19" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0806">Transcription termination</keyword>
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    <keyword evidence="6" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
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            <begin position="186"/>
            <end position="258"/>
        </location>
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            <begin position="402"/>
            <end position="568"/>
        </location>
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            <end position="812"/>
        </location>
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            <begin position="88"/>
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        <location>
            <begin position="1153"/>
            <end position="1195"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="32">
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            <begin position="1177"/>
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        </location>
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        </source>
    </evidence>
    <sequence length="1264" mass="139912" checksum="70D514AD1D25A664" modified="2024-11-27" version="1">MGDIKNFLYAWCGKKKLTPNYDIRAAGNKNRQKFMCEVRVDGYSYIGMGNSTSKKDAQSNAARDFVNYLVRIGEISASEVPALGASVPDVPDGSGQEGGGFGNLPANGPLPPHLVVKNESVADKASAPVPGVTGLGYSGFGNSQWERGANLQDYYSKRDEQDAKATLESEEVDLNAGLHGNWTLENAKARLNQFFQKEKIQTDYKYSQVGPDHNRSFIAEMTIFVRQLGRKIFAREHGSNKKLAAQSCALSLVRQLYHLSVIEAFTGQTKKKEGETVEAYDVNVSEDLKQQLQSVINELCVHIPPPDPSGPVSLVQGKMAHFEPSQRQSMAGVVPWSPPQVNWNPWTSSNIDDGPLAFCTPEQISSDLQQELSYQLEHDKDLQAMMHEREQLPVKKFEEEIMNAVHRNPVVIIRGATGCGKTTQVPQYILDYYIQGGRASECNIVVTQPRRISAVSVAERVAFERGEEVGKSCGYSVRFESILPRPHASVLFCTVGVLLRKLEAGIRGISHVIVDEIHERDLNTDFLMVVLRDVVQAFPDVRIILMSATIDTTMFREYFFNSPVIEVFGRTYPVQEYFLEDCIQMTQFVPPPMDRKKKDKDEEGGEEEVNCNLVCDPEYGPETKRSMAQLNEKETPFELIEALLKYIETLQVAGAVLVFLPGWNLIYSMQKHLEMSPHFGSQRYRILPLHSQIPREEQRRVFETAPEGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTSHNNMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFEKLETHMTPEIFRTPLHEVALSIKLLRLGGIGQFLSKAIEPPPLDAVIEAEHTLRELDALDCNDELTPLGRILAKLPIEPRLGKMMIIGCILNVGDAVCTISAASCFPEPFINEGKRLGFVHRNFAGTRFSDHVALLSVFQAWDEVRMGGEDAENRFCEHKRLHMSTLRMTWEAKVQLKDILINAGFPEECLMNQIFNNVGPDNNLDVVITLLTFGFYPNVCYHKEKRKILTTEGRNALIHKSSVNCPFSSHDMTYPSPFFVFGEKIRTRAISAKGMTMVSPLQLILFASKKITSDGETVELDDWIKLRIPHEVAGGVAALRAAVEALVVEVSKDPEYIRNMDPTNERLLNVIRLLSRPSSAGLNLMANNQRFGDGPRPPKMSRMDGDGGFRGRGGYQGNRGFRGGDGGGYGGGRGGGYGGGRGGGYRGGGGYGGGGFRGGYRGSGGYGGGGYRGSGGYRGGGGYGGGRGFNRGGYGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M6Q9</accession>
    <name>A0AAR2M6Q9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M6Q9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075069.1">
        <property type="protein sequence ID" value="ENSPNAP00000082896.1"/>
        <property type="gene ID" value="ENSPNAG00000037010.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214408"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000269">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="128"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="423"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="456" mass="52094" checksum="FD7929AE5189237C" modified="2024-11-27" version="1">MLSLLIRKFVHSGEWPADVTKLHPDVALMKRQSSKLMEKGGLVYSVTQKNSDGEIHQLLLPEKFREQVIKSVHDDMGHLGVERTLELVRNRFYWPRMSLTVELYVKNCGSCVAWKSPCIRAAPLHQIVTSGPMELVCIDFLCLEPDSKGFSNILVVTDHFSRYSQAYPTKDQRAVTVAKTLVEKFFVHYGLPSRIHSDQGRDFESRLIHELLRVLGIRKSRTTPYHPQGDPQPERFNRTLLSMLGTLGSTQKRQWSQHVSQLVHAYNSTKNDSTGYSPYFLMFGREARLPVDVCFGAEEREGVSHHRYVEELRQDLQNAYELAAKAASRVHLRNKRGYEKLLRPQVLDTGDRVLLKNLGLKGKHKLQSRWSSLPYVIVGKIPDLPVYRIKPESGMGRVRTIHRDHLLPIGFRRSSVSSILCQPSHTERRGSEPFVNPGRLNLQQPKQLPAGSEPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQS0</accession>
    <name>A0A3B4BQS0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription initiation factor TFIID subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">TBP-associated factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TAF3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TAF3 forms the TFIID-A module together with TAF5 and TBP. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The TAF3-TBPL2 complex replaces TFIID at specific promoters at an early stage in the differentiation process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TAF3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037398265.1">
        <property type="nucleotide sequence ID" value="XM_037542368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQS0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002052"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011091.2">
        <property type="protein sequence ID" value="ENSPNAP00000002052.2"/>
        <property type="gene ID" value="ENSPNAG00000001072.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427025"/>
    <dbReference type="GeneTree" id="ENSGT00710000106806"/>
    <dbReference type="OMA" id="ENIHMRQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901797">
        <property type="term" value="P:negative regulation of signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048634">
        <property type="term" value="P:regulation of muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22916">
        <property type="entry name" value="HFD_TAF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15522">
        <property type="entry name" value="PHD_TAF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000056">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000317">
        <property type="entry name" value="transcription initiation factor TFIID subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46452">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46452:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="836"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="134"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="368"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="422"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="755"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="500"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="554"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="577"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="604"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="643"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="670"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="770"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="784"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002052.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="900" mass="99560" checksum="41FA75F3D6E0A141" modified="2024-11-27" version="2">MCESYARSLLRVSVAQVCQALGWDAVQLSACDLLSDVLERYLQQLARGCHRYSELYGRTDPGLSDIDQAFGLMGVSIAELEDYINNLEPVAFSQSVPQFPISKSSVLQFPAAGFETDARNALRGERREYIPEYFPPLVSLQEDEEEEEQVSADMGTSAEAMQVSLGEEEDGEDEENDENWLGQRRSDSPRPEGLLPAAKRPRLGTKPGSSPEWSFEPREPLSSLNAQHMPRSPSPPLTIEPARPTVAPPTPSHKPKIPSLSPARQKNKSPKRGGGSASSGVSARPLALTPPKSAQKAGKRSPGRPRSPRSPRPGPANLVPAQFGRGWAGLHAEVVANDGQFKGHDAKEEAIEDSIAAVIERVRKDEAGPVDPFAYDSGSDSDGLPSPPKIGPKHMQGLNMPKQPLVPSKHAHILGAKQLATPLAPSLPAPSRWTMDDSIDEVIRKANQSQPVASQSPSEPAYFSSPSESPPTPQPPLKPLQERKDPTATPTLLKKKLKKDVKTKLKKKEKERLKEKGKDKGKNKEKNKEKKREKERTGQMEENKMPWPELSLGMDERRDNAGGGSKKEKDKHKDKKKDKEKSKKDKEKRDKGKERGKEERKLSLGAKEAAVAAPAASLPLFSPSSCLRIPSMLPPLQPILPEKLFHPKETKSKEKDKKKDKKEKKKKKDKEKEREKEREKDREKERAREKERDKEEKKKEKEKEKKEKAKEKEKEKPKLEKPAVMVEPPAVPQSPVIPRLTLRVGAGQDKIVISKVSSDLEPTPPLPRTPVARAGPGARVRALPPLPPPAPAPVASPPPPPPVLQPQPSQAKARGCSVVTETVSAYVIRDEWGNQIWICPGCNKPDDGSPMIGCDECDDWYHWPCVGIIAAPPEDQSWFCVKCAGKKKDKKTKKRKRKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUV4</accession>
    <name>A0A3B4BUV4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">COMMD10</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUV4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002361"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010567.2">
        <property type="protein sequence ID" value="ENSPNAP00000002361.2"/>
        <property type="gene ID" value="ENSPNAG00000008776.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001500"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="CDD" id="cd04758">
        <property type="entry name" value="Commd10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037361">
        <property type="entry name" value="COMMD10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12333">
        <property type="entry name" value="COMM DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12333:SF0">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="129"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002361.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="267" mass="30353" checksum="4297A12A9ED85D73" modified="2024-11-27" version="2">MAAIAKETQSIKEAVGHINQVDANKFGRLLSRILQKLHLKGERSFSEDEEQKLQTALAMEKQALHLVLETTSFLLEQAVYHNIKPASFKQQLENIHIAPDKAEAFCQAWATAGPDVVEKIRQRIFAPKKLDHIGWQLNLQMAQSSQAKLKAPHAVLDLGVKEEDESEHLQHVFVEFSHQELLEFYNKVRLDSFSFPHDCLANTVRHDTHLQTVKHSFFSLFSSPFAARDDSSTVGFPYLMLEPTFMSKSAVYSPHFHCNGVFKSDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVI0</accession>
    <name>A0A3B4BVI0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TCTN1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the tectonic-like complex (also named B9 complex).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tectonic family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566735.1">
        <property type="nucleotide sequence ID" value="XM_017711246.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVI0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002500"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010328.2">
        <property type="protein sequence ID" value="ENSPNAP00000002500.1"/>
        <property type="gene ID" value="ENSPNAG00000008877.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435415"/>
    <dbReference type="CTD" id="79600"/>
    <dbReference type="GeneTree" id="ENSGT00570000079101"/>
    <dbReference type="OMA" id="AGDVKIC"/>
    <dbReference type="OrthoDB" id="2104337at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904491">
        <property type="term" value="P:protein localization to ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040354">
        <property type="entry name" value="TCTN1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011677">
        <property type="entry name" value="TCTN1-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057724">
        <property type="entry name" value="TCTN1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611">
        <property type="entry name" value="TECTONIC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611:SF1">
        <property type="entry name" value="TECTONIC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25752">
        <property type="entry name" value="DUF1619_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07773">
        <property type="entry name" value="TCTN_DUF1619"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017338752" evidence="7">
        <location>
            <begin position="20"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3 N-terminal" evidence="9">
        <location>
            <begin position="110"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3" evidence="8">
        <location>
            <begin position="245"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3" evidence="8">
        <location>
            <begin position="427"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="634" mass="68435" checksum="A60E1ABB11829BCC" modified="2018-12-05" version="1">MAAVFCAFLLFTCLVKTLCSSDADSVRFNATFSDTNRTWFDWEGSLNVTERPEGENGTLAPFSMPAAAPAEPSPAFPEEEAPEPTARPDLARVATAALPLPLSGALPLPVTRASDLCPCNVQSEQCDVNCCCDPDCSQEQALFTHCSVKKVTGDYKLCSQDAALYTLSTTPEGLSQVRTSFQQEFNPDVFCIQSANYEQGMSFVTPSTPTEQNFDYLFGHFVGFFFGSSSVTGSALETPVQGNSPGYLYGDIIQTEEEAGQRGFFRLPASAGTANCLDTNPAAFLKDQTSRCMRSFALAEDCTTLEALKLRTYTSFRIFSGKDTEANLVAMWVEAVTLQSLEGTQTLLETAADSAFEPEFLQPADVCNQVVLQVKYTVRYGEAGQIVRVAVSFVLGAVNSAMLPIQQEFQIAFVQEAKSDTMLRSSGNPGYVVGLPLVAGWRTAEGIIQSSNPEGSLTVLQSSADQDCLFSFPQRSPVLFGKDVVSGCTFRLEDGANCSLVSEAILGRLKGQSFPDYVASFGNSLPQNTMDWVPIGNQTTITGTQGCNIPLSYHLEVRWTKYGTLVNPQAQIVSVVEIIRFNTSTLSSLSTGGTIVPVTSSVSFIDVSASASPGFRVPPTINAKLPFDFFFPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVT9</accession>
    <name>A0A3B4BVT9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="14">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DDX24</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase that plays a role in various aspects of RNA metabolism including pre-mRNA splicing and is thereby involved in different biological processes such as cell cycle regulation or innate immunity. Plays an inhibitory role in TP53 transcriptional activity and subsequently in TP53 controlled cell growth arrest and senescence by inhibiting its EP300 mediated acetylation. Negatively regulates cytosolic RNA-mediated innate immune signaling at least in part by affecting RIPK1/IRF7 interactions. Alternatively, possesses antiviral activity by recognizing gammaherpesvirus transcripts in the context of lytic reactivation. Plays an essential role in cell cycle regulation in vascular smooth muscle cells by interacting with and regulating FANCA (Fanconi anemia complementation group A) mRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="14">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with FADD. Interacts with RIPK1; this interaction disrupts RLR signaling activation of IFN-dependent transcription factor IRF7. Interacts with NIP7. Interacts with EP300; this interaction prevents TP53 acetylation mediated by EP300.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX24/MAK5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017546374.1">
        <property type="nucleotide sequence ID" value="XM_017690885.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVT9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002619"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010133.2">
        <property type="protein sequence ID" value="ENSPNAP00000002619.2"/>
        <property type="gene ID" value="ENSPNAG00000008956.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423520"/>
    <dbReference type="CTD" id="57062"/>
    <dbReference type="GeneTree" id="ENSGT00550000074847"/>
    <dbReference type="OMA" id="QMIQKAR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17946">
        <property type="entry name" value="DEADc_DDX24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001059">
        <property type="entry name" value="ATP-dependent RNA helicase DDX24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="264"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="594"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="89"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="358"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="358"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002619.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="880" mass="99305" checksum="E0ACA3F6031375A9" modified="2024-11-27" version="2">MKTKNSQSKKRFRSAKKRGILVKGKWQTVKIDPSVFADEGMSEIVCFEELTDYSLIESEKTATVSQLLQEPVGKKKRKRKACEIENYDEEETFEEAQEEFAELSSDDNIEQVDIVEKPDKKRKKKKKKSKSDAADFEMKHQDNNKNMESSSEGLSNLADKGEEQITAQTTLKSKNKKKKKKQDKSDAEQTRPSSQVKETDGQIGPVNKTKRKTKNWADAALSHSAGQDTDVSAWKDLFVPAPVLKALSKLGFSAPTPIQALALPPAIRDHMDILGAAETGSGKTLSFGIPMIHTILEWKKTINCKNAGADGKSNTEEDTEPGFNKAAGIAMNADNSQENSSAQEEGSLNDEDEGIILESAEDGDVGGQTVEEDDDDSGDDDDCGSVDQSQDEDGLTNDDESIGLDIKTMGDVKGDGEKKPLLGLVLTPTRELAVQVKHHIDAVAQFTGIRTALLVGGMAPQKQDRMLKRKPEIVIATPGRLWEMVQDKHPHLRNLRQLRCLVIDEADRMVEKGHFAELEKLLEILNTSQFNPKRQTFVFSATLTMVHSLPTRLLQKKGKKLEERSKLELLIEKVGIKGKPKVIDLTRKEATVETLTETRIHCEKEEKDFYLYYFLMQYPGRTMVFANSIDCIKRLTSLLTILDCTPLPLHANMHQKQRLKNLERFAERESCVLLTTDVAARGLDIPDVQHVIHYQVPRTSETYVHRSGRTARAAKEGLSLLLIGPDDMMNFKKIYRTLGKDEDLPLFPVQNKCMTAIKERVDVARKIEKIEYHNSREKQHNSWFRQAAEEMDIDLDDDLLLGAGRDENEEREQQKLVKGLKKHLKHLITQPVFKPHMKTKYPTQMGKLQLPELPLANETALISVTNQRRKQNQQKRKGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXT9</accession>
    <name>A0A3B4BXT9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="12">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CCT7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BXT9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004507"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006898.2">
        <property type="protein sequence ID" value="ENSPNAP00000004507.1"/>
        <property type="gene ID" value="ENSPNAG00000001475.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428579"/>
    <dbReference type="CTD" id="10574"/>
    <dbReference type="GeneTree" id="ENSGT00550000074832"/>
    <dbReference type="OMA" id="HRKGNTW"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000045">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000049">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="593"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="616" mass="67438" checksum="58F15AD03B1D7293" modified="2018-12-05" version="1">MKKVTSYNRTDCSLKTLHHCFTSVPRCRAAVFGAAAAPPAVHWENRHGPCSREFPSALWEPAGLPLAAKMMPTPVILLKEGTDTSQGVPQLVSNINACQVIAEAVRTTLGPRGMDKLIVDNRGKATISNDGATILKLLDVVHPAAKTLVDIARSQDAEVGDGTTSVTLLAAEFLKQLKPYVEEGLHPQTIIRAFRIATQLAVKKIKEIAVTIKKDDKQEQRQLLEKCAATALNSKLIAGQKDFFSKMVVDAVMMLDDLLQLKMIGMKKVQGGALEDSQLVAGVAFKKTFSYAGFEMQPKRYENPKIALLNIELELKAEKDNAEVRVNNVEDYQAIVDAEWNILYDKLEKIYKSGAKVVLSKLPIGDVATQYFADRDLFCAGRVQEEDLKRTMMACGGSIQTSVGSLTDDVLGLCELFEEVQVGGERYNFFKGCPKAKTCTIILRGGAEQFMEETERSLHDAIMIVRRAIKNDSIVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEVIPRQLCDNAGFDATNILNKLRAKHAQGGMWYGVDVNNEDIADNFQACVWEPAIVRINALTAASEAACLILSVDETIKNPRSSVDAPPVPAGRGRGRGRPPHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYX9</accession>
    <name>A0A3B4BYX9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Neurotrophin receptor associated death domain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="nucleotide sequence ID" value="XM_037536800.1"/>
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</entry>
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                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
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    </evidence>
    <sequence length="296" mass="33079" checksum="EE65DE4774A8CD17" modified="2018-12-05" version="1">MSVANARQTIFNPVVPFTGTIAGGLHPGEMVVIQGCVLNEADRFQFDLSCGSSTKPRADIAFHFNPRFRSPPSIVCNSLQQGSWGREEKLDQMPFRQGTSFETIILVHENVFKVAVNGAHVLEYKHRIPLERVDTFSVSGKVKIQAIGYVPNSAIFSESGDLSIPYKGSILKGLRPGQHITIKGHVSLFPHSFTVNLRCSQSENIALHLNARIKSGMFVRNSFLSQSWGPEELELLCFPFSAGKYFEIIILCQSHQFKIAVNGAHLLDYRHRVQDLSCINQLEVLGDLELQDVKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6T8</accession>
    <name>A0A3B4C6T8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase kinase kinase kinase</fullName>
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        </recommendedName>
    </protein>
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        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017560834.1">
        <property type="nucleotide sequence ID" value="XM_017705345.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C6T8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007582"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001582.2">
        <property type="protein sequence ID" value="ENSPNAP00000007582.2"/>
        <property type="gene ID" value="ENSPNAG00000002774.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431883"/>
    <dbReference type="CTD" id="692287"/>
    <dbReference type="GeneTree" id="ENSGT00940000166658"/>
    <dbReference type="OMA" id="ALIHANC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06613">
        <property type="entry name" value="STKc_MAP4K3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000031">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021160">
        <property type="entry name" value="MAPKKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF30">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038172">
        <property type="entry name" value="MAPKKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="15"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="15">
        <location>
            <begin position="558"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="409"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="498"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="21"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007582.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="898" mass="99853" checksum="87010CA4B9C184A1" modified="2024-11-27" version="2">MDAIGVLHTNPLDDYELLHRIGSGTYGDVFKARNNKTSVISAIKVVKLDPGDDISSIQQEITMMKDCTHKNIVAYFGSYLRNNKLWICMEFCGGGSLQDIYHVTGPLKERQIAYVCRETLQGLHHLHETGKMHRDIKGANILLTERGDVKLADFGVAAEINASVAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDIWAVGITAIELAELQPPMFDLHPMRALMLMSKSSFQPPKLKDKTKWTTEFHNFIKMALTKSPRKRPTAEKLLQHAFVTQLLTRNLVIELLDSANNPDLQHSQTMDDSDLETCSAFPDKITSLGKHLSAQRTHSEEQFDQVKFGPPMRKETDPCPDSPGSCDDWSSISDEKESPSLLECVEEALLERSLTIKRVPSAESPTEDDKYGTVKRVVSPPQPAILQCPTNQDKKNSTPSSDSSPCTGITPHTDTNSSLLNESALLIGSTLSDLSLLTNSSFFANSSLFTDSSSLGSLCTTPTDGLISRGSVAVEPGGNRSHSPPRQPLSPEWSTLRRKTEDSRGVHGLPPTPQVHMGACFSKVFNGCPLKIQCAVTWVLPKTRDQHLILGSEEGIYTLNLNELHEDTIEKLLPQRCSWLYVMNNVLMSVSGKSSQLISHSLPALFEHRRNLQRRQGHLSINAHRLSTRINSRKYAMSVKIPDTKGCRRCSVVRNPYTDSVFLCAAVPSGLVLLMWYEPLQKFMQLKHIALSLPESLPIFELLVQESEELPQVCVGVHSSHKKSVETVEQLHFDIIHLDDTPREQPAGESLEVKQVTQLDRDTVLIALENTVRAVNLQGCPSKGMASELTFSFSIETLVCLQDSVLAFWKHGLKGRSIQSHEVTQEITDESRVFRVLGTNRDIILQSTPTDDPSALSNLYILTGHESSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9G7</accession>
    <name>A0A3B4C9G7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554155.1">
        <property type="nucleotide sequence ID" value="XM_017698666.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017554156.1">
        <property type="nucleotide sequence ID" value="XM_017698667.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037401724.1">
        <property type="nucleotide sequence ID" value="XM_037545827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9G7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008273"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000421.2">
        <property type="protein sequence ID" value="ENSPNAP00000008273.1"/>
        <property type="gene ID" value="ENSPNAG00000013892.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428008"/>
    <dbReference type="CTD" id="492363"/>
    <dbReference type="GeneTree" id="ENSGT00940000154651"/>
    <dbReference type="OMA" id="WQLTMIS"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000009">
        <property type="entry name" value="Low affinity cationic amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004755">
        <property type="entry name" value="Cat_AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00906">
        <property type="entry name" value="2A0303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF20">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="496"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="561"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="590"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="15">
        <location>
            <begin position="559"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="623"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="644" mass="69841" checksum="C63A166E2C51880B" modified="2018-12-05" version="1">MVDKMASVGRMLLRRRVLDCSGEETRFARCLSTLDLVALGVGSTLGAGVYVLAGEVAREKAGPAIVLCFLVAALSSMLAGLCYAEFGARVPKTGSAYLYSYVTVGEIWAFITGWNLILSYVIGTASVARAWSSTFDNLIEQKISSFFKSFMAMKVPGNVLAEYPDLFALVLILLLTGLLAFGVSESALVNKIFTGINLVVLGFVIMSGFVKGNTANWNLTLDNFINDTNITSPEKVENSFGSGGFAPFGFSGILSGAATCFYAFVGFDCIATTSEEAKNPMRSIPVGIVASLLICFFAYFGVSAALTLMMPYYKLNTQSPLPEAFSYVGWAPARYIVAAGSLCALSTSLLGSMFPMPRVIYAMAEDGLLFRALSSMNKRTKTPLMATVVSGFVAALMAFLFDLAALVDLMSIGTLLAYSLVAVCVLILRYQPGTLNSSSQSEKLVELVGGEKVAVSGDSGDEYSPDSEDHIPREKFFAKLLLFPSRDVPTKMSGTIVYATTAIISVLITLLCVVLAVNLDELLVGHPIWVTVCIVLALLCFFCVIIIWRQPESKEALTFKVPLLPWLPLFSVFVNIYLMMQLDLATWCRFAVWMGIGFLIYFLYGIWNSTEALNSKQRKYVPSTKNKSPIYLGGDESEVEGISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CA47</accession>
    <name>A0A3B4CA47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Lipoxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CA47"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007776"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001281.2">
        <property type="protein sequence ID" value="ENSPNAP00000007776.2"/>
        <property type="gene ID" value="ENSPNAG00000002827.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156796"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000001">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="15">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="16">
        <location>
            <begin position="171"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="12">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007776.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="70342" checksum="8B26F6EA97394A75" modified="2024-11-27" version="2">NWHNDVIIYTENDALTADSISTAPSYYLHVQNFTVECKVNIGKLLLVELEAKSPSFMTFIDDDWFCSKITVTTPQGACMLFPCHSWMSCEKKLILRDSVGLVKTCNNTMYCQASDSFMCYVFTFYRWSIFSPGLPHVVLAESPNELPPEVRFSFTKEKELLFTKEYVQKNWRKDEFFGYQFLNGLNPMIIKRCSKLPEKFPVTNEMVRGFLPDTSTLESEMAKGNIFICDYERLRDLEGNEINKKKQYLAAPLCLLFSSQGKLMPIAIQLNQQPGRGNPIFLPSDSETDWLLAKIFVRNAEFCEHELNFHLLRTHLIGEVFTVATMRNLPSCHPLFKLLLPHTRYTLQINVMARNRLISVDGYFAKFTAIGNESMTILMKRAASSLTYSSLCLPDDIKERGLEKIPNYYYRDDGLELWNIMYKFVFGFVSHYYSDQDIQEDKELHAWLTEMRENTLLDNSEAGFPESFYTVKELAKFVTMMIFSVSAQHAAVNNGQFDFGGWMPNFPSSLRCPPPSRKGSTTKDSLLATLPDIRTTVHALAVGYMLSQGSSDRYPLGHYPEELFSEEVPLKLIAQFKNDLQDLEKKIDARNKKLDLPYTYLNPRNVENSVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFU3</accession>
    <name>A0A3B4CFU3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein phosphatase 1E</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Ca(2+)/calmodulin-dependent protein kinase phosphatase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">CaMKP-nucleus</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Partner of PIX 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Partner of PIX-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">PPM1E</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="RefSeq" id="XP_037403107.1">
        <property type="nucleotide sequence ID" value="XM_037547210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFU3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009741"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016211.2">
        <property type="protein sequence ID" value="ENSPNAP00000009741.2"/>
        <property type="gene ID" value="ENSPNAG00000015287.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411118"/>
    <dbReference type="GeneTree" id="ENSGT00940000157884"/>
    <dbReference type="OMA" id="SHLRYHY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000021">
        <property type="entry name" value="Protein phosphatase, Mg2+/Mn2+-dependent, 1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF535">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 23" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="25">
        <location>
            <begin position="138"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="398"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="405"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009741.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="623" mass="69873" checksum="72901CB44E931BBB" modified="2024-11-27" version="2">MAACGSEEKTYRRFLELFLREMRPPLPEDGPLPTRPLSDSVAEDEVEGETLDLCLQHLCKYNCPFTLASALARGTADNILQSDLSVYYLNKNVEDGTETLPQIEAVKLARLVFNKLCETCCLWLKGIPTRRHVQPYYETSIHAIKNMRRKMEDRHVVIPDFNALFNLQDQEEQAFFAVFDGHGGVDAATFAANHLHVNLVRQEMFSQDPGEALCRAFKLTDERFVQKASRENLRCGTTGVVTFLRGRTLYVAWLGDSQAMLVKKGQPVELMKPHKPDREDEKQRIEALGGCVIWFGTWRVNGSLSVSRAIGDSEHKPYICGDADRITFPLDGTEDYLILACDGFYDTVNPDEAARVVSDHLQENNGDTTMVAHKLVASARDAGSSDNITVIVVFLRDPRAPPPSEEPEEEGEEPGEEPVEEAEPEQEEEEEEETSQSELVCQDGGGENGGKSRAGWPLQQCSAPADLAYEDRMDSFTDRTSLSITGPEPFSEKGCFRMPPEAQGPPVRTFWPDLGAGGGRVQRPHLETEGYCGLRHQKWPKSQVPSFTNPMTGQWLEAAALFPQQGRRKRRMEGVVLRREMKKRRILMARELCCPSSPHPNVPYPLRCIESRPGVALPRVTHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CHS7</accession>
    <name>A0A3B4CHS7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CHS7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016593.2">
        <property type="protein sequence ID" value="ENSPNAP00000010044.2"/>
        <property type="gene ID" value="ENSPNAG00000003396.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234960"/>
    <dbReference type="OMA" id="THPDPLF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010044.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="106" mass="11807" checksum="625653D790701E02" modified="2024-11-27" version="2">MEPSLGLLLLVPGIITPLEPLELSQRRRRRLVQQQRHGLGLLRLGHQHGMAAQHDGLVLQLVTIDPGKYLGQAWIGHTVGDPVQQRCPDTAPCTWGHSLGTTWQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CLC0</accession>
    <name>A0A3B4CLC0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SPACA4/bouncer family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017562660.1">
        <property type="nucleotide sequence ID" value="XM_017707171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CLC0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012145"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036399.2">
        <property type="protein sequence ID" value="ENSPNAP00000012145.1"/>
        <property type="gene ID" value="ENSPNAG00000017869.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432975"/>
    <dbReference type="CTD" id="100538040"/>
    <dbReference type="GeneTree" id="ENSGT00390000002843"/>
    <dbReference type="OMA" id="IRFPLRC"/>
    <dbReference type="OrthoDB" id="8919691at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035036">
        <property type="term" value="P:sperm-egg recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046354">
        <property type="entry name" value="SPACA4/Bouncer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47613">
        <property type="entry name" value="SPERM ACROSOME MEMBRANE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47613:SF1">
        <property type="entry name" value="SPERM ACROSOME MEMBRANE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017462043" description="UPAR/Ly6 domain-containing protein" evidence="10">
        <location>
            <begin position="20"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="11">
        <location>
            <begin position="21"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="126" mass="13618" checksum="10093FE8C31F2367" modified="2018-12-05" version="1">MSSRLSSLLLLCMLPSVVPLFCYTCVFPAISPLDCIRYPTRCPPGQRCLSSKAIGQRGDFQVVLYEKSCVLPSLCGIAGEKFAMGLNFTFTNECCDTHLCNGATTSARGVYTGTLLSLSLSLLFSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CLI8</accession>
    <name>A0A3B4CLI8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">STE20-related kinase adapter protein alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">STE20-related adapter protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">STRADA</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552975.1">
        <property type="nucleotide sequence ID" value="XM_017697486.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552976.1">
        <property type="nucleotide sequence ID" value="XM_017697487.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CLI8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011701"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018755.2">
        <property type="protein sequence ID" value="ENSPNAP00000011701.1"/>
        <property type="gene ID" value="ENSPNAG00000017397.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427392"/>
    <dbReference type="CTD" id="92335"/>
    <dbReference type="GeneTree" id="ENSGT00940000158827"/>
    <dbReference type="OMA" id="INGVMPF"/>
    <dbReference type="OrthoDB" id="840771at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000130">
        <property type="entry name" value="STE20-related kinase adapter protein alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000213">
        <property type="entry name" value="STE20-related kinase adapter protein alpha isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
        <property type="entry name" value="STRAD_A/B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE FRAY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF20">
        <property type="entry name" value="STE20-RELATED KINASE ADAPTER PROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="61"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="299"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="428" mass="47747" checksum="777BCBAA98679864" modified="2018-12-05" version="1">MSFLRWVSEKLSVESLRDLELFGEQAQASPLRKAHEDSRESLSPLPHRDTMGTFLPDSSAYELLTIIGRGLEDLMTVNLARYRPTGEHVAIRRIDLESCTNDMVNYLQGELHVSKLFHHPSILPYKSIFIAENELWVITPFMAYGSARDLISTHFTDGMSELTIAYILHGVLRALEYIHHMGYVHRSVKASHILISADGQVYLSGLRSIFSLIRHGQRARVVHDFPQYSVKVLPWFSPEVLQQNLQGYDFRSDIYSLGITACELANGHVPFKDMPATQMLLEKLNGTVPCLLDTTTIPPEELSMKPSRSGADSGICEGPGAGGARHTNGEPSSSSGANPYSRTFSSHFHAFVELCLQRDPEKRPSASTLIGHSFFKQIKRRPCEALPDLLRPVSPLSTFECVQPQDSPSGLASLESGLSHLDVDDWDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CML8</accession>
    <name>A0A3B4CML8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Period circadian protein homolog 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Circadian clock protein PERIOD 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PER2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000012598"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036586.2">
        <property type="protein sequence ID" value="ENSPNAP00000012598.1"/>
        <property type="gene ID" value="ENSPNAG00000018376.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156342"/>
    <dbReference type="OMA" id="FHGGKMM"/>
    <dbReference type="OrthoDB" id="7788983at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000013">
        <property type="entry name" value="Period circadian protein homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000004">
        <property type="entry name" value="Period circadian protein homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057310">
        <property type="entry name" value="PER1-3_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048814">
        <property type="entry name" value="Per1-3_PAS-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022728">
        <property type="entry name" value="Period_circadian-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050760">
        <property type="entry name" value="Period_circadian_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269:SF9">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bHLH_PER"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="387"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="1240"/>
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        <location>
            <begin position="42"/>
            <end position="57"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00039684"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042893"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012598.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
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    <sequence length="1413" mass="154774" checksum="74CB3D715F03E8BA" modified="2018-12-05" version="1">MSSESEPKPYLFRAGSEEEETEFTCGSMPHLHRMGGASDGSDSSHDPGARPPLHDDIEMGGNGSSGSSTESHGNELHGNESHCNESVGSSNGNGKDSAFLESSGSNKSSNSQSPSPPSSSNAFSMVSSEQDHPSTSGCSSEQSAKEKTQKELFKTLKELKLHFPADKRSKGRASTLHTLRYALRCVKQVKANEEYYQMLMINDSQPPGLDVSSYTIEEINRITSEYTLKNTDIFAVAVSLITGKIVYISEQAAGILHYRKDEFKDRKFVEFLTPQDVSVFYSFTTPYRLPAWSMCTGAESSSTEFMREKSFFCRISGGKEKEGGLQYYPFRMTPYLMKVQDAELSEEHFCCLILAERVHSGYEAPRIPPDKRIFTTTHTPNCVFQDVDERAVPLLGYLPQDLIGTPVLLHLHPNDRPLMLAVHRKILQYMGQPFDHSSIRFCARNGEYVTIDTSWSSFVNPWSRKVSFVIGRHKVRMGPVNEDVFSAPAFTEGKIMDSDIQEITEQIHKLLLQPVHSLGSSGYGSHGSNGSHDHLLSVASSSDSNSNGNMATGNSNTGQEEGSKTKPRTFKEICKGVQMLKTQDQQVSTAKSDQKKSAETGLKAPALKQKDAALVVSPGGTAAVGELRDQTLYSYQQISCLDSVIRYLESCNVPITVKRKYQFSSSNTTSSNSDEEKKGSDGALQSLQDPRSSKKPLAGPAAVVSGTLAPLPLPTKPESVVSITSQCSYSSTIVHVGDKKQPESEIIEDVAESPAPPAPVSVVSPPTVEQGAYRKLGLTKQVLAAHTQKEEQAFLSRCKELCQSWTLQKDGPANAARNRGQANDGLPGPHASKPGISAAEPTTKRSGRTRKSKKPRINKPDSSDSAVSQRMRRPPLQSLNQTSWSPSEASQSAFSVPYPTLVPGYPLYTAAPPPATTLPPSGERNPAGSFAEAPNAQVAPTAPPYPAPLVTPVVAFVLPNYVFPQLSTAPRPPFYAEPQQAYTNQQPFQHQPTVNPQQALQPQQNYTTQQPFQPPQPQQAYSTQQPFQPQQAYATQQLFQPQPAYNTQQPFQPQQTYTVQQAFQPQTAYSSQQPFQPQQGYTAQQPFQPQQPFDNQQQTFQPQTPFNPPQPFQMQTQFTAQTPFPPYRITSDPKPPANEAPDGPSLPPSLHSPPLFGSHCSSPLQLDLLQLEDKPDSHSTAAPSGSQGICSSALEKSNVVSSSATAAAGEAQQSCRLGDGSRSDGHSSSSDLMELLMQEDSGSATSASTGSASNGCNTSNSGTGSSNTSKYFASIDSSENDHKDKRKKDGQNFRYVLQDPLWLLMANADDKVMMTYQVPARDMEKVLRDDRERLRRMQKSQPRFTSDQRRELIEQHPWMRRGGLPKAIDVKECLYCEDDAPLEEDLPDMDLGILGAETVQEEGVAQIQEEAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPE5</accession>
    <name>A0A3B4CPE5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diamine acetyltransferase 1</fullName>
            <ecNumber evidence="5">2.3.1.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Polyamine N-acetyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Putrescine acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Spermidine/spermine N(1)-acetyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an alkane-alpha,omega-diamine + acetyl-CoA = an N-acetylalkane-alpha,omega-diamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11116"/>
            <dbReference type="Rhea" id="RHEA-COMP:9766"/>
            <dbReference type="Rhea" id="RHEA-COMP:9767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:70977"/>
            <dbReference type="ChEBI" id="CHEBI:70988"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:11117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>spermidine + acetyl-CoA = N(1)-acetylspermidine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28150"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:58324"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:28151"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>spermine + acetyl-CoA = N(1)-acetylspermine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33099"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45725"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58101"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33100"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.57" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017540561.1">
        <property type="nucleotide sequence ID" value="XM_017685072.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPE5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012414"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019656.2">
        <property type="protein sequence ID" value="ENSPNAP00000012414.1"/>
        <property type="gene ID" value="ENSPNAG00000018180.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412845"/>
    <dbReference type="CTD" id="431716"/>
    <dbReference type="GeneTree" id="ENSGT00950000183121"/>
    <dbReference type="OMA" id="NIQWQTP"/>
    <dbReference type="OrthoDB" id="7305308at2759"/>
    <dbReference type="UniPathway" id="UPA00188">
        <property type="reaction ID" value="UER00363"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004145">
        <property type="term" value="F:diamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019809">
        <property type="term" value="F:spermidine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009447">
        <property type="term" value="P:putrescine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032918">
        <property type="term" value="P:spermidine acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000011">
        <property type="entry name" value="Diamine acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051016">
        <property type="entry name" value="Diverse_Substrate_AcTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545:SF36">
        <property type="entry name" value="DIAMINE ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="170" mass="20028" checksum="9B7A7140D01BDF0D" modified="2018-12-05" version="1">MEQFILRNAEPKDVSDILRLIKELAKFEEMEEQVVLTEKELLEDGFGEHPFYHCVVAEVPEEKQTPEGYRVVGFAMYYFTYDPWIGKLLYLEDFYVMKEFRGYGIGSEILKRLSETAVKCRCSSMHFIVAEWNKQSIEFYKRRGAADLSHEEGWRLFEIGKQSLIKMSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPW4</accession>
    <name>A0A3B4CPW4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 180</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017556039.1">
        <property type="nucleotide sequence ID" value="XM_017700550.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPW4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012821"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036677.2">
        <property type="protein sequence ID" value="ENSPNAP00000012821.1"/>
        <property type="gene ID" value="ENSPNAG00000003540.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429071"/>
    <dbReference type="CTD" id="100142644"/>
    <dbReference type="GeneTree" id="ENSGT00940000164377"/>
    <dbReference type="OMA" id="TACMCCT"/>
    <dbReference type="OrthoDB" id="62987at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000380">
        <property type="entry name" value="Transmembrane protein 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040035">
        <property type="entry name" value="TMEM180"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28658:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN CONTAINING 13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28658">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="54027" checksum="452C4B4AA24ED25A" modified="2018-12-05" version="1">MLQILRKLFSAHKNIMKHLRISAHPAAVAYSMTTLGSSMMNSIFSFYYVKLFLNKYRISEAAFHQSQVVYMIWNALNDPLFGYLQDNSNIQCCSLRRLSILYGAPFYSLAFLLTWFPWRDYAPEDWLSGVHLVVTLCAFDGMLTFVLLAQCALFAEISEHHHNRVRLIKYNQVASLLGSSSVLLCGLVSNNMDNFAAFQGFCFLVAVLGCICMVYTGLHSEGRFDRKYSDTDSQQPALSLSSVITMTWQIISNRDFQLFVVMNFFQVFMLAFCNNFAMIFVDHLIPADVLPSLAKSFMYGAGFICPQLLVLSSQNLLHVFGYYRLILMTFYVEAAAAALMLLLGPNHHYCLALFITANMILVQSAFSLFGLPLADIIDADLEKYKRSSPLSSMVFGTNALFTKPGQSLAPMLVVAILNQYGYEDIKDGKVTEPSAVNALHNIMFYLICIVPMCVTAVQVIAWKPFSIRNSHTTDSKYTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CS49</accession>
    <name>A0A3B4CS49_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SDCBP</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CS49"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014927"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022981.2">
        <property type="protein sequence ID" value="ENSPNAP00000014927.1"/>
        <property type="gene ID" value="ENSPNAG00000020907.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436145"/>
    <dbReference type="GeneTree" id="ENSGT00940000164997"/>
    <dbReference type="OMA" id="GIHEYQD"/>
    <dbReference type="OrthoDB" id="10059177at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045545">
        <property type="term" value="F:syndecan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06721">
        <property type="entry name" value="PDZ1_syntenin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000043">
        <property type="entry name" value="Syntenin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000065">
        <property type="entry name" value="syntenin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051230">
        <property type="entry name" value="APP-Binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345:SF18">
        <property type="entry name" value="SYNDECAN-BINDING PROTEIN (SYNTENIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345">
        <property type="entry name" value="SYNTENIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="124"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="208"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="308" mass="33109" checksum="0FB200003C9BB32A" modified="2018-12-05" version="1">MSLYPSLEDMKVDKFIQAQNTPTASPSKALALSDPAHCLSTPAHHESKVEEIGTGTSGGLYPELSEFMGLNLSTDALQTLSTTVPEQSSGALSVHSSGMNWAAAPITGGDLGVKRAEIRQGIREVVLCKDMDGKIGLRLKAIDNGVFVQLVQANTAASLGGLRFGDQILQINGESCAGWTSDRAHKALKNANPERITIAVRDRPLERTVTLHKDMSGQLGFIFKKGRITSIVKDSSAARNGLLTDQQICEINGQNVIGLKDSQVLDILNCSGRVVTVTVMPVVIFEHMMKRMCSSIVKSLMDHSIPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTQ2</accession>
    <name>A0A3B4CTQ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZDHHC2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017562526.1">
        <property type="nucleotide sequence ID" value="XM_017707037.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTQ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014775"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022772.2">
        <property type="protein sequence ID" value="ENSPNAP00000014775.1"/>
        <property type="gene ID" value="ENSPNAG00000020766.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432903"/>
    <dbReference type="CTD" id="51201"/>
    <dbReference type="GeneTree" id="ENSGT00940000153716"/>
    <dbReference type="OMA" id="CFVVMHI"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="125"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="379" mass="44009" checksum="1F836BBC5887592F" modified="2018-12-05" version="1">MAPSGSRNIKRDCRRVLYWIPVLFICLIVAWSYYAYVVQLCIETIENLGEKIVYLVAYHVFFIMFVWAYWQTIFTKPMNPLKEFHLSHLDKELLEREDRGESQQEILRRIAKDLPIYTRTTSGAIRYCERCHLVKPDRCHHCSVCDKCILKMDHHCPWVNNCVGFSNYKFFTLFLAYSLLYCLFVTATDLQYFIKFWTVDGKTHERLKEYWNGLPDTQAKFHIMFLFFASSMFSVSLASLFSYHCWLVCKNRSTLEVFRAPAFRHGTDKNGFSLGVSKNLRQVFGDQKKYWLLPIFSSQGDGCSFPTCLVNADPEQPASPSGHAAINSDEDTHQFPAKPLRESQSRLLSNGQTWTDSEGTEDKDREGTSNPAMTIEKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTS1</accession>
    <name>A0A3B4CTS1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EFEMP1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570734.1">
        <property type="nucleotide sequence ID" value="XM_017715245.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037388985.1">
        <property type="nucleotide sequence ID" value="XM_037533088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTS1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014146"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021965.2">
        <property type="protein sequence ID" value="ENSPNAP00000014146.1"/>
        <property type="gene ID" value="ENSPNAG00000020082.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437849"/>
    <dbReference type="CTD" id="2202"/>
    <dbReference type="GeneTree" id="ENSGT00940000157837"/>
    <dbReference type="OMA" id="CETRNEC"/>
    <dbReference type="OrthoDB" id="10022113at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055088">
        <property type="entry name" value="Fibulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF28">
        <property type="entry name" value="UROMODULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017238736" description="EGF-like domain-containing protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="142"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="183"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="223"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="263"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="79"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="461" mass="51449" checksum="8699B2D910D4754D" modified="2018-12-05" version="1">MLGLCVILAVLARTVAQDAEEPVSYTCTDGYEYDPVRQVCKDIDECAIVADACKGGMKCVNHFGGYLCLPQNAQIFVSSGDEPSQPAEPSAPANPPQSPGQGQSEVQTHVQPGRGPGRRVSSSRILRCTPGFILDEQNYCRDIDECTRGRHTCSADQICYNTRGSFLCQCQPGYQKNGDQCVDRDECLLSHYCMHRCVNTPGSYYCECNSGYRLASNNHSCVDVNECQVSQPCEHQCHNLIGSFICQCNRGFELAPDSVSCIDIDECAYSPYMCQYACINTPGEYSCTCPDGYQLQGTRMCQDINECETSHECTEEEMCWNYYGGYRCYPRNPCEDPYVRTSENRCVCQPTTACRGGPQSIVYKYMSIHSDRTVPADIFQIQATNIYPNTHNTFRIRAGNEKGEFFLRRSSNVSAMLVLTKSLEGPLELIVDLEMITHHTVANYRTSSLLRLTIIVGPYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWA1</accession>
    <name>A0A3B4CWA1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Myosin, heavy chain 6, cardiac muscle, alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MYH6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000014814"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022805.2">
        <property type="protein sequence ID" value="ENSPNAP00000014814.2"/>
        <property type="gene ID" value="ENSPNAG00000020778.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167286"/>
    <dbReference type="OMA" id="IQWNLRS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003012">
        <property type="term" value="P:muscle system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000004">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000006">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000013">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000007">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000115">
        <property type="entry name" value="Myosin heavy polypeptide 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF69">
        <property type="entry name" value="MYOSIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 17" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 17" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11 17" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="20">
        <location>
            <begin position="32"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="19">
        <location>
            <begin position="85"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="17">
        <location>
            <begin position="653"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1905"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1921"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="178"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014814.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1935" mass="222477" checksum="EF2A56082220ED4B" modified="2024-11-27" version="2">MGDVIMAEFGKAAPYLRKSEKERLEAQTRPFDIKSECFVVDEKVEYVKGQITSKEGGKVTVKTEDGRTVTVKETDVHPQNPPKFDKIEDMAMFTFLHEPAVLFNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYDAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSVLITGESGAGKTVNTKRVIQYFASIAAAGGAAKKDSNKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGTSGKLSSADIETYLLEKSRVTFQLKAERNYHIFFQILSSAKPELLDMLLITNNPYDYSYISQGEVTVASINDSEELLATDNAFDVLGFTPDEKMGVYKLIGAIMHYGNMKFKQKQREEQAEPDGTEAADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQGVDQVYYSLGALAKAVYEKMFNWMVARINQSLDTKQHRQYFIGVLDIAGFEIFDFNTFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIDWEFIDFGMDLQACIDLIEKPLGIMSILEEECMFPKASDQTFKAKLYDNHQGKSNIFQKPRAVKGKAEAHFALGHYAGTVDYNITGWLVKNKDPLNETVVGLYQKSSLKLLTHLFSSYAGAEAAEKSGKGAKKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCLIPNETKTPGAMDNCLVMHQLRCNGVLEGIRICRKGFPNRVLYGDFKQRYRILNASAIPEGQFIENKKSAEKLLGSLDIDHTQYKFGHTKVFFKAGLLGTLEEMRDEQLARIITRLQANARALLMRAEYQKLVERRDALMVIQWNLRSFLSVKNWPWMKMFFKIKPLLKSAEAEKEMANMKDEFNKLKEALDKSDGRRKELEEKMVTLLQEKNDLLLQLQSEQDTLTDAEERCEQLIKSKIQLEAKVKELTERIEDEEEINADLTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMASQDEMIMKLTKEKKALQEAHQQTLDDLQSEEDKANTLAKAKAKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKMAQENAMDLENDKQQQEEKLKKKDFEICQLNGKIEDEQMAVVQLQKKLKENQARIEELEEELDADRAARAKVEKQRSDLSRELEDISERLEEAGGATSAQVELNKKREAEFQKLRRDLEESTLQHEATSAALRKKHADSVAELGEQIDNLQRVKQKLEKDKTELKLELDDLASNMESIVKAKVNLEKICRSLEDQMNEYRTKAEEAQRALTDFSTQRAKLLTENGELGRQLEEKECLISQLTRGKSSYTQQLEDMRRQLEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRALSKANSEVATWRAKYETDGIQRTEELEEAKKKLVQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMLDLERSNAASAALDKKQRAFDKVIAEWKQKYEESQCELEASQKEARNLSTELFRLKNAYEECLDQLETIKRENKNLQEEISDMTDQLGEGGKNVHELEKLRKQLEQEKAELQSALEEAEASVEHEEGKILRAQLEFNQVKADIERKLAEKDEETEQAKRNYQRMVESLQSSLEAETRSRNEALRVKKKMEGDLNEMEIQLSQANRQAADAQKQLKMVQSYLKDTQLQLDDSVHMNDDLKENAALLERRNNLLQAELEELRAVVEQTERGRKLAEQELTDATERTQLLHSQNTSLLNQKKKQESDLLQLQTEMEEVIQEKRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQVAMKGGKKQLHKLEARIRDLENELEAEQKRGTESIKGVRKYERRIKELTYQTEEDRKNMARIQDLVDKLQLKVKSYKRSAEEAEEQANVNLAKLRKLQHELEEAEERADIAESQVNKLRAKTRDGVPGKKSQDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2I8</accession>
    <name>A0A3B4D2I8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2I8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039017.2">
        <property type="protein sequence ID" value="ENSPNAP00000018552.2"/>
        <property type="gene ID" value="ENSPNAG00000024970.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018552.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9591" checksum="DE56EA510B127F4D" modified="2024-11-27" version="2">YCIKIFLLTETRKQHVPAFPLVLKEGVFFVINSVFKVMLRDVMALQAEKMNHHPECFNVYNKVITSTELPLHGPGAVYKASRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3B0</accession>
    <name>A0A3B4D3B0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MSC</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3B0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018030"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027060.2">
        <property type="protein sequence ID" value="ENSPNAP00000018030.2"/>
        <property type="gene ID" value="ENSPNAG00000005944.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426547"/>
    <dbReference type="CTD" id="9242"/>
    <dbReference type="GeneTree" id="ENSGT00940000160438"/>
    <dbReference type="OMA" id="MISESYW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF67">
        <property type="entry name" value="TRANSCRIPTION FACTOR 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="55"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018030.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="152" mass="16522" checksum="38BF2809D4F654C6" modified="2024-11-27" version="2">MSTGSVSDPEDEVCCGRVESSGATTPPLCRSVDSEDDDVGSAACEGVKSQPSRRVQRSAANARERARMRVLGRAFTRLRTTLPWVPPDTKLSKLDTLRLAASYITHLRQLLQEDTHTSTLHPPALSWPFMVSGRPDETKEISAALRLCGATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4P8</accession>
    <name>A0A3B4D4P8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SH3 and multiple ankyrin repeat domains protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Proline-rich synapse-associated protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">May homomultimerize via its SAM domain. Interacts with BAIAP2, DBNL and SLC17A7/VGLUT1. Interacts with DLGAP1/GKAP, GRM1/MGLUR1, GRM5/MGLUR5 and LZTS3 C-termini via its PDZ domain. Interacts with ABI1, HOMER1, HOMER2, HOMER3 and CTTN/cortactin SH3 domain. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts (via PDZ domain) with the GRIA1 subunit of the AMPA receptor (via PDZ-binding motif). Interacts with WASF1 and CYFIP2; the interactions mediate the association of SHANK3 with the WAVE1 complex. Interacts with ARPC2; the interaction probably mediates the association of SHANK3 with the Arp2/3 complex. Interacts (via ANK repeats) with SHARPIN and SPTAN1. Interacts (via PDZ domain) with ARHGAP44 (probably via PDZ-binding motif); the interaction takes place in dendritic spines and promotes GRIA1 exocytosis. Interacts with CAMK2A. Interacts with DIP2A. Interacts with ADGRL3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017574377.2">
        <property type="nucleotide sequence ID" value="XM_017718888.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000017879"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038724.2">
        <property type="protein sequence ID" value="ENSPNAP00000017879.2"/>
        <property type="gene ID" value="ENSPNAG00000024195.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440064"/>
    <dbReference type="CTD" id="557701"/>
    <dbReference type="GeneTree" id="ENSGT00940000153561"/>
    <dbReference type="OrthoDB" id="445896at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030160">
        <property type="term" value="F:synaptic receptor adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17177">
        <property type="entry name" value="FERM_F0_SHANK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06746">
        <property type="entry name" value="PDZ_SHANK1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09506">
        <property type="entry name" value="SAM_Shank1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000029">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000006">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000025">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000018">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000048">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000064">
        <property type="entry name" value="SH3 and multiple ankyrin repeat domains protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051569">
        <property type="entry name" value="SHANK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135:SF4">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9 17" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 18" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <feature type="repeat" description="ANK" evidence="17">
        <location>
            <begin position="277"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="17">
        <location>
            <begin position="344"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="17">
        <location>
            <begin position="377"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="584"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="22">
        <location>
            <begin position="692"/>
            <end position="786"/>
        </location>
    </feature>
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        <location>
            <begin position="1865"/>
            <end position="1928"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="56"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="469"/>
            <end position="579"/>
        </location>
    </feature>
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        <location>
            <begin position="790"/>
            <end position="810"/>
        </location>
    </feature>
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        <location>
            <begin position="922"/>
            <end position="994"/>
        </location>
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        <location>
            <begin position="1034"/>
            <end position="1053"/>
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        <location>
            <begin position="1070"/>
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            <end position="1345"/>
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        <location>
            <begin position="1373"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1760"/>
            <end position="1847"/>
        </location>
    </feature>
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        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="485"/>
            <end position="524"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="525"/>
            <end position="534"/>
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        <location>
            <begin position="535"/>
            <end position="548"/>
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        <location>
            <begin position="943"/>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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            <dbReference type="ARBA" id="ARBA00004552"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="ARBA" id="ARBA00023273"/>
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        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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            <dbReference type="PROSITE" id="PS50105"/>
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            <dbReference type="PROSITE" id="PS50106"/>
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            <dbReference type="Ensembl" id="ENSPNAP00000017879.2"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1928" mass="209737" checksum="53702DFF2B6CC530" modified="2024-11-27" version="2">MPVSAGAGKHEPPQPRKQRPEQQRERQPEPQQPESTAPFLPLSLPLSLPHLSLPRLIHSRPAPPQPELHRLSSTASQGDSSRRSHGDGCRAPAGPMEEPQGNTLVVRIGIPDLQQTKCMKFNVEAPIWLSKQRILCTLNQSLKDVLNYGLFQPAYNGKAGKFLDEQRTLKEYPLPAITPIPYLEFRYKRRVYTQAHLDDKQLSKLHTKANLKKFMEYVQQRNIEKVSKFLEKGLDPNFHDPETGECPLTMAAQLEGCAELIKVLKGGGAHLDFRTRDGITALHKAVRTKNHTALITLLDLGASPDYKDSRGLTPLYHSSMVGGDPYCCELLLHDHAQVGCVDENGWQEIHQACRHGHVQHLEHLLFYGADMSAQNASGNTALHICALYNQDSCARVLLFRGANKEIKNYNSQTAFQVAIIAGNFDLAEIIKVHKASDVVPFRETPSYTNRRRVTGGGTLTSSRSLLRSASDNNLNGADVGPTHSPVPSLRSLPPLAPPGGEAADGSLQSTGSSRSSHSRSPSLHRVSEEPDIHTLRRHPQHPHGHSRGRLSPGTVQRDPSPPPSAPTHVLSGSRGPKRKLYSAVPGRTFIVVKPYTPQGEGEIQLNRGERVKDYHSNAVEVLSIGEGGFWEGTVKGRTGWFPAECVEEVQMRQYDPRLETREDRTKRLFRHYTVGSYDNFTSYSDYIIEEKDAVLQKKENEGFGFVLRGAKAETPIEEFTPTPAFPALQYLESVDLEGVAWRAGLRTGDFLIEVNGVNVVKVGHKQVVSLIRQGGNSLLMKVVSVTRKPESEDVVRKKAPPPPKRAPSTTLTLRSKSMTAELEELASARRRRGERLDEILASQESVLKPQVEADYRAATVKQRPTSRRITPAEISSLFERQGLALHGALHPALEKAHMQLPKGMSRTKSFGATEEDRLSALAAEHRFPRSSSMTDSLRDSHSIPPPPQTAPPPPPSLYYLDTGPPPAFCPPPPPPSRLHDPSRSSFKPGSEPRLHALPQTMSAEMYEPPRHAAHIERQKKARSMIILQDSTHLPVEPTDIPRPGPASTPPERIKRKGRVIDNPYANVGQFSMGLYTPPPSKPQRRKSPLVKQLQVEDAQERASLALASVHAREHSPTGRLVAHHTSRAEYYQQQLLQERTRAQAEGLLQGKGPFAAAIAGAVKDRERRLEERRKSTVFLSVGTMEGSSATPVEAPSLTQSHSVDERLLTSELGHLPPPALALRPSPGGTTFIHPLTGKPLDPNSPLALALAARERALTSQSQSPASSPEPRTKHEAAGGVLLMDTQTKEAERSEGEGELASPPFSPGGKRSYTPAPSSKIQWGTPTTARKEMEIGADKKDERKVEDKKSMIISIMDTSQQKTAGLIMVHATSNGQAVGLGPDRVSPPPKGMRTESPKPMLAEIKRAKSPSPEAASTATPTPTTPSTPQGLPGPSSDKPLQPSSSPSSDKTLAQGSSEEDVEPYTVTLPPAMLSSSDEETREELRKIGVVPPPDEFANGILAKAPEAAVPPSLPSAGKQTVPSDSAQAPAPSTAAAATAAQAPAAIGPPAASGKPSEPHLGPESAADSGVEEVDTRSSSDHHLETTSTISTVSSMSTLSSESGEPTDTYTSHADGQTFILDKPPVPPKPKLKSQLSKGPVTFRDPLLKQSSDSELLSQQHAAALAAATGASGPPRPRYLFQRRSKLWGDPIEPRPLAGAEEGKPTVISELSSRLQQLNKDTRSLGEEPLGGSLDPGRKSPVAGARLFSSLGELHTISQRSYGTTYTIRPGSRYPVTRRTPSPGSGSPDRSDPLGPIRGFGLATSPTTPPTILKSSSLSLPHEPKEVRFVMRSSSARSRSPSPAHSPGLPAPLLTLRPFHQKPLHLWNKYDVGDWLVSINLGEHRERFQEHEIEGSHLPALTKDDFAELGVTRVGHRMNIERALKQLLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5A8</accession>
    <name>A0A3B4D5A8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5A8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038817.2">
        <property type="protein sequence ID" value="ENSPNAP00000018094.1"/>
        <property type="gene ID" value="ENSPNAG00000024453.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163304"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="CD59"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050918">
        <property type="entry name" value="CNF-like_PLA2_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914:SF9">
        <property type="entry name" value="COILED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="UPAR/Ly6" evidence="3">
        <location>
            <begin position="56"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="149" mass="15421" checksum="B11114C6E3D1C27C" modified="2018-12-05" version="1">MGAGAEHTIRTKDCARAAECVSGSLNLGVMKMSLNTQCCTSDRCNTLPFGSPNGKKCFMCTDKDCSGTVSCEGDQDRCVSATVTANGVQVAMKGCVSRNFCTTDASSMQAAGITGSVSCCEGNLCNGAEGVKLSLLIMLVPLIFSTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5N1</accession>
    <name>A0A3B4D5N1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">BCAP31</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575917.1">
        <property type="nucleotide sequence ID" value="XM_017720428.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5N1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018421"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027615.2">
        <property type="protein sequence ID" value="ENSPNAP00000018421.1"/>
        <property type="gene ID" value="ENSPNAG00000024799.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441095"/>
    <dbReference type="CTD" id="10134"/>
    <dbReference type="GeneTree" id="ENSGT00390000011863"/>
    <dbReference type="OMA" id="CEGQKDK"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000011">
        <property type="entry name" value="B-cell receptor-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041672">
        <property type="entry name" value="Bap31/Bap29_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF15">
        <property type="entry name" value="B-CELL RECEPTOR-ASSOCIATED PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18035">
        <property type="entry name" value="Bap31_Bap29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="14">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Bap31/Bap29 cytoplasmic coiled-coil" evidence="15">
        <location>
            <begin position="188"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="247" mass="27943" checksum="9FF1972486882EB7" modified="2018-12-05" version="1">MSLQWTAVATFLYAEVFAVLLLCVPFVSPKRWSKIFKSRLVVAVTTYGNTAFIVVICILVFLLVDAFREVRKYSVTDKVDLTNNPVAIEHIHMKLFRAQRNEYIAGFALLLCLLLRRLASLLSQQATLMASNEAFQKQAEGASDAARKYMEENEKLQEKLREAGLEVPEVGGKTKASAEEENKTLKGEVRQLKDELDATRKALQKSDSDVKAMKKQAENLTVEYDRLLEEHAKLQAKLDSQQDKKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6N2</accession>
    <name>A0A3B4D6N2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAR14-like first RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6N2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020022"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030254.2">
        <property type="protein sequence ID" value="ENSPNAP00000020022.2"/>
        <property type="gene ID" value="ENSPNAG00000036739.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175544"/>
    <dbReference type="OMA" id="RIKSHEP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057051">
        <property type="entry name" value="PARP14_RPM_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23222">
        <property type="entry name" value="RRM_PARP14_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23085">
        <property type="entry name" value="RRM_PARP14_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAR14-like first RRM" evidence="2">
        <location>
            <begin position="13"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020022.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="208" mass="23510" checksum="4049669E7885FD29" modified="2024-11-27" version="2">MDEYLHPLSVEAVEGRWPSASAKTLTAKLQIYFQSRRKSQGGDCVVRFSEQSRARGDWYGSQKRKRDIHPSSLDPAEESLKDCLEEPPQSNIVALEKLPDSFSKDFLVLLVEKISGVPEKDHLFFLFLFAVAEKFLVESRSHKKFQQYGVRGRALEKSGSVKVESLHGEVSADLLEVYFEKCTGLVENVTMIPEEQAAIVTFHSEEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9V2</accession>
    <name>A0A3B4D9V2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574060.1">
        <property type="nucleotide sequence ID" value="XM_017718571.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9V2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020280"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039934.2">
        <property type="protein sequence ID" value="ENSPNAP00000020280.1"/>
        <property type="gene ID" value="ENSPNAG00000004178.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439903"/>
    <dbReference type="CTD" id="792915"/>
    <dbReference type="GeneTree" id="ENSGT00940000160411"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16990">
        <property type="entry name" value="ENTH_Epsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF103">
        <property type="entry name" value="EPSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="2">
        <location>
            <begin position="12"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="490" mass="52675" checksum="F99866831A5085AA" modified="2018-12-05" version="1">MTQSMLRRTLKNLVQNFSEAEVKVREATSNDPWGPSSSQMSDISDLTYNVVACGEIMGMLWKRLNDDKNWRHVYKSLTLLEYLLKTGSDRIPKQCQENIHTVKALTEYRFMDKDGKDQGVNVREKAKIVLVLIENEEKRKEERDFAMKTKDKLATASVDPNEKAKAEIPPYTGLPSLDNIPSVADLTASMAKKKEEQRRLEEKRKEEERRAKEGDRDPDLWEQAGTTGPPSGSDPWGAPSDSLKDTGPATNDPWGAPSDNSKDSDLANNDPWGGSADGKKDSAAASSDPWGGPADAEKQSSPAKDDPWGESSEPTEDKGQASNDPWGASDDLPVESQPQKSDPWGSPTDFPEESAPGSEPSGSEPSAAKPDPWSSDVGTSAVSPTDPFGDGAKADNDPWGTTASPLPANDPWGAPAVPPHGTAVDDPFGDGGNSSAWGTSTERDGKKPGSFLGEGASLVDLDSLMSVKPKPKQPSLNSILSSGEKAQGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBK7</accession>
    <name>A0A3B4DBK7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037398784.1">
        <property type="nucleotide sequence ID" value="XM_037542887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBK7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020833"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040221.2">
        <property type="protein sequence ID" value="ENSPNAP00000020833.2"/>
        <property type="gene ID" value="ENSPNAG00000004258.2"/>
    </dbReference>
    <dbReference type="GeneID" id="119264354"/>
    <dbReference type="CTD" id="50616"/>
    <dbReference type="GeneTree" id="ENSGT00980000199676"/>
    <dbReference type="OMA" id="QEDSCCI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020453">
        <property type="entry name" value="IL-22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48488">
        <property type="entry name" value="INTERLEUKIN-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48488:SF1">
        <property type="entry name" value="INTERLEUKIN-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14565">
        <property type="entry name" value="IL22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043501173" evidence="1">
        <location>
            <begin position="22"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020833.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="173" mass="19805" checksum="A4E71E7BC92E2E5D" modified="2024-11-27" version="2">MMMKNLLALLSLLSCLALSAASPRPLRHPPRSPPLDSSETWNNLFTMCKDARAQDKDHETRLIPRIPSEKMKDGTMCCGNVKILEYYLNHILHQVTGSYSRMDLVRHELRRVARDLEPHCRSNLAGHEHFQSFTENLQLAGQMYKSNATAHNKAIGETDILFHYLYETCKPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DC04</accession>
    <name>A0A3B4DC04_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho GTPase-activating protein 42</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rho GTPase-activating protein 10-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Rho-type GTPase-activating protein 42</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May influence blood pressure by functioning as a GTPase-activating protein for RHOA in vascular smooth muscle.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557446.1">
        <property type="nucleotide sequence ID" value="XM_017701957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DC04"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021942"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033720.2">
        <property type="protein sequence ID" value="ENSPNAP00000021942.1"/>
        <property type="gene ID" value="ENSPNAG00000029320.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429891"/>
    <dbReference type="CTD" id="569534"/>
    <dbReference type="GeneTree" id="ENSGT00940000155492"/>
    <dbReference type="OMA" id="CIRMVEM"/>
    <dbReference type="OrthoDB" id="3183924at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01249">
        <property type="entry name" value="BAR-PH_GRAF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000161">
        <property type="entry name" value="Rho GTPase activating protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000114">
        <property type="entry name" value="Rho GTPase activating protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000001">
        <property type="entry name" value="Rho GTPase-activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000008">
        <property type="entry name" value="Rho GTPase-activating protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047225">
        <property type="entry name" value="PH_GRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF3">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="265"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="14">
        <location>
            <begin position="395"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="844"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="598"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="790"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="641"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="689"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="711"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00081471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="902" mass="101678" checksum="4FBFC44F072F51DF" modified="2018-12-05" version="1">MGLPTLEFSDAFLDSPDFRDRLKCHEIELERTNKFIKELIKDGNSLIGALKNLSVAVQKFSQSLQEFQFECIGDAETDDEVNIAQSFKELSQLLNSVEEERRRLIQNADDVLITPLEKFRKEQIGAAKEGKKKFDKETEKYYSTLEKHLNLSSKKKESLLQEADTQIDKERQLFYDASLEYVFKIQEVQEKKKFEFVEPLLAFLQGLFNFYHEGYELAHEFEPYKQQLQFNLQNARNNFLSTKQEVEKLMKRVRSADQDQKPPGQWTMEGYLYVQEKRPLGCTWTRHYCTYEKGSKTFVMTNTEAKATAKQNGVVLNPTEMFKLKSCIRRKTDSIDKRFCFDIEVVERNAICRGTLFRPLHYFCKVRHGIVTLQALSESNRRLWLEAMDGKEPIYNLPAILSKKEETHLNEAGFNFVKKCIGMVEMRGINTMGLYRIGGVNSKVQKLMTTVFSPKAPADMDLDPDTWDNKTITSGLKNYLRCLSEPLMTYRLHEDFIQAVKSDDQNYRVCAVHALVHKLPDRNKEMLEILIKHLLIVSTHSKSNMMTVANLGVVFGPTLMRSQEETVAAMMNIKFQNVVVEIMIENFEKIFHQAPDPNVALPQAQSRPESRRSRAICLSTGPRKPKSPYTPTLCLADADSDTFSSSSGSTPMGSMESLSSHSSEQNSSSKTGSSSQLKEKASLMVLPRSPSSLSSNGPQGLACTSVSSRESSSREDTPRTDSEDSRSLSSVPTASRSSLAPKKAPHSSTIAQRSAQFTCLPAPSLRSLHNLEVSARRSCAGSVQSLLAMDHRDSSRTAPHPDLPPKRLLRHRPDSSNGYQRPGSVVAARALLFENPSSAATASPVGRDAKAMYSCEAEHSNELSFPQGAHFSNVYPSVEPGWLQATYEGRTGLVPENYVVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCR0</accession>
    <name>A0A3B4DCR0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase RING2</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RING finger protein 1B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">RING finger protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">RING-type E3 ubiquitin transferase RING2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">RNF2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
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        <subcellularLocation>
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        <subcellularLocation>
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    </comment>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-C3HC4_2"/>
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        <property type="entry name" value="RING"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0678">Repressor</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="51"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="158"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="176"/>
            <end position="190"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="336" mass="37466" checksum="EFE5958E4FF72477" modified="2018-12-05" version="1">MTQTVQTNGVQPLSKTWELSLYELQRTPQEAITDGLEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLARISKHNNQQALSHSIEEGLKIQAMNRLQRGKKHQIENGSGAEDNGDSSHCSNASVHSNQEAGPSIKRTKTSDDSGLDMDNATENGGGDMALDGASEIELVFRPHPTLMEKEDAAQTRYIKTSGNATVDHLSKYLAVRLALEELRKNGGASPINVEAASEKQYTIYIPTANNQFTVLNGSFSLELVSEKYWKVNKPMELYFAPTKEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDG5</accession>
    <name>A0A3B4DDG5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017560314.1">
        <property type="nucleotide sequence ID" value="XM_017704825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDG5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022462"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041236.2">
        <property type="protein sequence ID" value="ENSPNAP00000022462.1"/>
        <property type="gene ID" value="ENSPNAG00000029837.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431573"/>
    <dbReference type="CTD" id="556445"/>
    <dbReference type="GeneTree" id="ENSGT00390000010463"/>
    <dbReference type="OMA" id="EYQQDWK"/>
    <dbReference type="OrthoDB" id="9995210at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000002">
        <property type="entry name" value="glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000019">
        <property type="entry name" value="Glutaminase liver isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041541">
        <property type="entry name" value="Glutaminase_EF-hand"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF33">
        <property type="entry name" value="GLUTAMINASE LIVER ISOFORM, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17959">
        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Glutaminase EF-hand" evidence="8">
        <location>
            <begin position="80"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="610" mass="67571" checksum="07B89866A4B50637" modified="2018-12-05" version="1">MNSLKTLVRTTSVGVFELFAKDALKSTVRGRRPGVWSQKRRFSHGSSAGIHLSTDRAAGGGEPLPAPCGGRGEGLFSGLEDLLFYTITEGEERVPISHFIAALKSTGLLTSDPRLRDCMEKLRQAVRECTGEVMMDRELFRKFVGGNIVLLSLAFRKKFIIPEFEEFVAIINQIYNRAKEEDDGQVADYIPHLAKFSPSLWGMSLCTVDGQRHSAGDTKVPFCLQSCVKPLEYAIAVHEQGTEHVHRFVGKEPSGLKFNMLSLDEDDKPHNPMVNAGAIVISSLIKPGSNKAEKFDYVMDFVKKMAGMEYVGFSNATFQSEKETGDRNFAIGYYLKEKKCFPCGADMIDALDFYFQLCSIEVTCESGSVMAATLANGGICPITGERVLTAEAVRNTLSLMHSCGMYDYSGQFAFHVGLPAKSGVSGAVLLVVPNVMGVMCWSPPLDKVGNSVRGIRFCQELVSLFNFHNYDNLRHFVKKQDPRRWSGEDRNKSVVNVMFAAHSGDVSALRRFALSSMDLEQKDYDCRTALHVAAAEGHVDVVQFLTQTCKVNPFVKDRWGNIPLDDAIQFAHTKVVKILQEYQQTYSVQSSPDVPDHIKQSKLGSVEGIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DK30</accession>
    <name>A0A3B4DK30_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DK30"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023289"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013006.2">
        <property type="protein sequence ID" value="ENSPNAP00000023289.1"/>
        <property type="gene ID" value="ENSPNAG00000007833.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158274"/>
    <dbReference type="OMA" id="GTHSMDE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046203">
        <property type="term" value="P:spermidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF405">
        <property type="entry name" value="PEROXISOMAL N(1)-ACETYL-SPERMINE_SPERMIDINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="507" mass="56709" checksum="19A9EF7199901BF4" modified="2018-12-05" version="1">MALRPAVDAKILIVGCGISGIGAAQRLIQHGFSNVRIIEATSRSGGRIKTGRLGSNIVEVGANWIHGPSQENPVFQLACHYQLLSKESMSEENQSADIGGHPPFVPNWFSSSGRKLDPDVMGPAVELFMTLFEKCQEFHASGEEPMPTVGEFLKREALRIAHEEWEEDQATKDLKLALLSTMLKLECCVSGTHTMDDVGLGAFGMYKTLPGLDCTFPGGYDGLIKHMMNELPADIVLYNKPVKCVHWNNTYKQSSSKGRTYPVTVECVNGETFEADHVIVTIPLGYLKKHQDTLLHPPLPLHKLHSIQRMGFGTNNKIFLEFEKPFWSEDCEVIYLVWDDEPDLVDVVSDVKTAWIKKLFGFTVLKPMERFGHVLCGWIAGHESEYMETLTEFEVLQAVTQLIRRFTGNPTIAPKKLLRSQWFHEPYTYGSYTYVAKGCSGYDIDNLAEPLPLPGSNSQPLQVMFAGEATHRSFFSTVHGALITGWREADRLHTHYTTLSGQMISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLB6</accession>
    <name>A0A3B4DLB6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HAUS augmin-like complex subunit 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLB6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023724"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011911.2">
        <property type="protein sequence ID" value="ENSPNAP00000023724.2"/>
        <property type="gene ID" value="ENSPNAG00000000824.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010974"/>
    <dbReference type="OMA" id="TAKMEHN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023724.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="41633" checksum="7439915BE8650E5D" modified="2024-11-27" version="2">MASKKSLNVRKVQKAAAEDSQNSASGNDTSCGNNSSGARRKTKSAGTVVKSRYMQTEKKASVAKNNMQNESILVPPRPASPKASGAPRPRVGTPPRRTLTRLSGQPDQNSTLMPSILETSTFGGNVLQSTVLDGHCLRPDFDLSVIKENTAPPSAVDPKSQKRNLELETFLLAFLTAKIEHNTHKLKEEAERNLLIMMEEEEKLRLKVMSKKRQYLLLEKHKQLNDLLDLQITALTPAAATAKQFTEEYKTFATAVDTTRHELPVKNLHIEEDRGKFLVVRTPMDPHRADKAVVCLNQSRRALEQYVKDASSGSESSVECLKEMRNTAREIDQQLLSASSELLELSSLVSRETVLAQQSLEEDKIGLTAAQALFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DML2</accession>
    <name>A0A3B4DML2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATG10 autophagy related 10 homolog (S. cerevisiae)</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DML2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024174"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010789.2">
        <property type="protein sequence ID" value="ENSPNAP00000024174.2"/>
        <property type="gene ID" value="ENSPNAG00000008675.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000924"/>
    <dbReference type="OMA" id="CGEMGQL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019787">
        <property type="term" value="F:ubiquitin-like protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000091">
        <property type="entry name" value="Zinc finger CCHC-type-containing 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042246">
        <property type="entry name" value="ZCCHC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46242:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46242">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 9 ZCCHC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024174.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="491" mass="55456" checksum="057D3E0D07208297" modified="2024-11-27" version="2">MTRWARANNVHKHKPTEATPWSQLRAGKPGRIKEIDTRTRRGSSSGPLAWDCASRSPRHIQPCPGRKRANNEREHDVNGFMDYLKQTQQTQQSLTLGPRKGASLGGDVATAIKKDKRSGIRRVKRQNAKKKNMVCFYCRKPGHGLANCPEADNDEELGRGICYRCGSTEHEIQTCRAKVDPALGDYANAKCFICGQTGHLSKSCPDNPKGLYAAGGCCRVCGSVEHFQKDCPEHQTSANSITLSRLSNQMSADQEDICVSVKKLPPKKTKMSDKEGSSAASCYLDEKSFHLSCQLFLQYSSVLKDGWSWTEFKGVKEGYMKKTILIPGRLCSLLNQFWKHKTEDVDALVEDQVDDDTENQAVCENQAVMCYEYHVLYSCSYQVPVLYFRASTLDGRLLSLEEVWNNIRPNYRKHLLQGPWDTLTQQEHPLLGQPFFVLHPCRTEEFMRPVLQMAHVEKRKVNYIVTWLSVMGPMVRLEVPLSYCTAVAAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DSC4</accession>
    <name>A0A3B4DSC4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ATP4B</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle.</text>
    </comment>
    <comment type="function">
        <text evidence="18">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">The ATPase pump is composed of two subunits: alpha (catalytic) and beta (regulatory). Interacts with alpha subunit ATP12A; this interaction is required for the formation of a functionally active pump and targeting at the plasma membrane. Interacts (via N-terminus) with alpha subunit ATP4A (via the P-domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DSC4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025859"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006539.2">
        <property type="protein sequence ID" value="ENSPNAP00000025859.1"/>
        <property type="gene ID" value="ENSPNAG00000001555.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429832"/>
    <dbReference type="GeneTree" id="ENSGT01030000234579"/>
    <dbReference type="OMA" id="APRVNCT"/>
    <dbReference type="OrthoDB" id="5912413at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000061">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01107">
        <property type="entry name" value="Na_K_ATPase_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF11">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00390">
        <property type="entry name" value="ATPASE_NA_K_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="12 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="291" mass="33786" checksum="1017E18FFC294ECF" modified="2018-12-05" version="1">MATLKEKRTCGQRCQDFGHFVWNSDDGTFMGRTPEKWVYISSYYVAFYVVMTGLFALSIYVLMYTVDPYYPVYQDRLKSPGVMVWPDMYNDEVLEITYNMSDKHSWMKMSSCLQHFLEPYNDTKQSHCNYYNCTSGKYFFQPKFDAPKHTKWSCPFYQSTLGNCSGIEDSTFGYTTAQPCVIIKMNRIINFLPNNGTGMAPYVNCTVLEGQENVRGHVYYPENGTMDLSYFPYYGNLAQPTYVNPLVAVKFDLINQRHAKIQCRVMANNIAYQNIYEPYEGKVDFHLTALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUS0</accession>
    <name>A0A3B4DUS0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">KIF22</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559975.1">
        <property type="nucleotide sequence ID" value="XM_017704486.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559976.1">
        <property type="nucleotide sequence ID" value="XM_017704487.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUS0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027200"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003204.2">
        <property type="protein sequence ID" value="ENSPNAP00000027200.1"/>
        <property type="gene ID" value="ENSPNAG00000002331.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431391"/>
    <dbReference type="CTD" id="3835"/>
    <dbReference type="GeneTree" id="ENSGT00940000159632"/>
    <dbReference type="OMA" id="VIREDRW"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01376">
        <property type="entry name" value="KISc_KID_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.280:FF:000002">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000043">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12836">
        <property type="entry name" value="HHH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10 13 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Kinesin motor" evidence="17">
        <location>
            <begin position="18"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="423"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="102"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="635" mass="70544" checksum="65F2636AECCC55E4" modified="2018-12-05" version="1">MAQRVAVNDAAGVKRTSRVRVAVRLRPYIGKEDEKGEGPCVRGLGPQTLEIINWRNATETLQYQFDVFHGEQTTQQEVFLSSVKPITPHILNGQNASVFAYGPTGAGKTHTMLGSQEHPGVIPRAVREVFQLVRIQERDQDEWQFSVGMSYLEIYNEKVLDLLSPSSQDLPIREDKDKNIVIPGLTHTPLSSFSDFDAHFVPASLNRTTASTKLNQRSSRSHAILLIKVVKSQRIPPHRQQTGKLYLVDLAGSEDNRRTGNQGIRLKESGAINLSLFTLSKVVDSLNAGAAGRVPYRDSKLTRLLQDSLGGSAHSVMITNIAPEYKFYFDTFTALNFAAKSKHIVNRPFVRETVMAPVNGKRAREEQEAGGSGEPQNKRLNVGKKAELPSPLHTHPHSPADSSVLDRLLALEKMMLGSPERERLNLLKTVAQSRREIQKLKEKQKELEEKASMLNKVAGGQSAVMGKKDSEAPLFRTDLNLLHRKESTAARPKKQQAVVTPLQVSQVQPLQQCAVVCKPSQALGKKKKHIQKISDGKENSSKEPSSGEEVNWESRLDSSLLEQSKQKILQTLNSGSLKELKSLQLIGDKKAKLILGWREINGDFTQMEDLKKIEGITAKRFSSFIKANILSAMGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DWA0</accession>
    <name>A0A3B4DWA0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DWA0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027753"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001750.2">
        <property type="protein sequence ID" value="ENSPNAP00000027753.2"/>
        <property type="gene ID" value="ENSPNAG00000013290.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00600000084617"/>
    <dbReference type="OMA" id="RWRMDYL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040647">
        <property type="entry name" value="SPIN-DOC_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052675">
        <property type="entry name" value="ZnF_transloc-Spindlin_int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34589">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 2700081O15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34589:SF2">
        <property type="entry name" value="ZINC FINGER TRANSLOCATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18658">
        <property type="entry name" value="zf-C2H2_12"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="378"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="506"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027753.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="586" mass="65842" checksum="B101D4CBD6B5BD13" modified="2024-11-27" version="2">MTCKKNEKIRLGFDADLLLYFSGEYDMTNGHIGDDGLVTPVRSPGTSYWSISESPNSPFLLSPIPGPSGQKATPAKPVRASRPGLSRIPGRDHRRYYHEYWRSEYLMDFDPRRHGMICMVCGSSLATLKLSTIKRHIRQKHPDSLLWSASDKEVIRSGWESHLNLENSQKPFCSDPAIATEPEETLDCAKRSTGCVRVGGCRKRQPQPRVTKASASPSASSVPEPQGPSAQMLERYLNDSLHAWFRQEFLMEYQAEAGRLVCMVCSCPLPSLHLDHIKSHVLELHPDSLVYSAEEKHCILQTWAQTHADSSVQFFPDVDPLQDSSEPHAGGKDEPPGAAQNPSTLPHQFPNRRLKNGCPWRLRLDYLVALGPPGSPGCYCMVCSEQLPVARVSGFRRHIQERHPETINLSRRERQDIAKELCLSLSFGKKVIPGDTTAEPAHVSTQTLSVKPAKQSAIKTEDGDRKTTPSTPHRHTHYPGKDQRRNYQVRWRMEYLMDYDCRRHGLICMVCGATLATLKVSTIKRHIQQVHPRSLDYSPEEKQQALQSYNQTALHFVHSDDCFSASDHGHGAHAKAKVEQTGCYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXJ9</accession>
    <name>A0A3B4DXJ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PP1-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ASXL1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXJ9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028155"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000757.2">
        <property type="protein sequence ID" value="ENSPNAP00000028155.1"/>
        <property type="gene ID" value="ENSPNAG00000013723.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000274174"/>
    <dbReference type="OMA" id="DSHRSEM"/>
    <dbReference type="OrthoDB" id="9947694at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051983">
        <property type="term" value="P:regulation of chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029334">
        <property type="entry name" value="PP1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21603">
        <property type="entry name" value="ANTIGEN KI-67-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21603:SF16">
        <property type="entry name" value="CELL DIVISION CYCLE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15276">
        <property type="entry name" value="PP1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PP1-binding" evidence="8">
        <location>
            <begin position="261"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="270"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="879"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="481"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="583"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="600"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="639"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="651"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="730"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="784"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="807"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="906" mass="99236" checksum="AAE2460CE654CF2A" modified="2018-12-05" version="1">MDGVEAVGSRTPLGTLTPSQQNSDGRETDFSKLTPSHFGISTDSFLTFSKEKDKSRVAQLKARRRSTIGLRGSPETNSLICFRAKAAMKTPPRTPQNILASPLFTGCDSIKQKMAAFQRLMGEDEENSEQSAPLMMKEEQGEGGMGGSLSKDVQTGNEVCSEERQDRITPSKRRPTATPPPSKKRRRVPLGLCEEEILEEPFSELQNTPTQEPGVRSSDFRSPELKLGLGSDSQSQLMSFPMLSKPEVIRTDDFEVSSVSKKKRVRFGAPLSPEFFDKTLPPSTPLQKGATPVRPPSSTGRKSLLKTPQRFDPPLPQPDFNSPGHNGASPVLSVSRRCRSREVESDEVFLDNEKITFPIMEDEFDNPPDDSKDADLAEGPELTLHLEPPAAGTELMNTGFREEEDLFPPFIADHSVALPAEVEAEPQPDLKQPESQQEQQPASPASTTEAPRSRGRKRKQPAQRENQTETKRSSRTAAASDKGKMKLPVEDVTEIRRSSRTAAESAKGKMKTTAAKKRFGSKEVDRSLYGKRNYASKNPLLSPIPESAASSLTNTPTHPHPDAKSDSSQTPAPNSHQRETDSTDATADLVAAAARWRQRFLQQPGKSGSATEVGHAIVEDDSASELEADPASADASVNTAAHTAGASSGTKARRTRRSSSRPAGRRRSGNTVRMKRLSGAHESETEGAVDEEQTRDTAGPLTESAIGDQMRQESTQSETLVRCESTQEEDNSHSALHQDKDEEPEPTAQRKQARKSPRVKSTAVTDVMEEEEPCQHPLETNQSGDERAEGEGGRELTESSLAVGDSEFSSVETLGGQNEPVLEPWQQANFNIEDILRPVVTRQRSVRRSLRNRRSMDVQASGLAWVDHTSPELITANRRRTRSRLSAVSQPPTFLDTEGAQANQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXK3</accession>
    <name>A0A3B4DXK3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SNX14</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037393350.1">
        <property type="nucleotide sequence ID" value="XM_037537453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXK3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028208"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000567.2">
        <property type="protein sequence ID" value="ENSPNAP00000028208.2"/>
        <property type="gene ID" value="ENSPNAG00000013925.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427503"/>
    <dbReference type="GeneTree" id="ENSGT00950000182856"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021680">
        <property type="term" value="P:cerebellar Purkinje cell layer development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06877">
        <property type="entry name" value="PX_SNX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08722">
        <property type="entry name" value="RGS_SNX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037436">
        <property type="entry name" value="SNX14_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037892">
        <property type="entry name" value="SNX14_RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF44">
        <property type="entry name" value="SORTING NEXIN-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="5">
        <location>
            <begin position="134"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="340"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="622"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028208.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="996" mass="114740" checksum="C6CAD70F31C24D74" modified="2024-11-27" version="2">MLRSQMADVRTLLRSLRRGFKIDMLREVVRQYPVVCFFLMSMLSSTIILNRYLHILMIFWSFLAGAITFYCSLSPDHLVPNVLFSVKTRKKRPEQQELFPLGHSCAVCGKNKCTRHRPTLLLEKYQPWLGLKVHSKVDASIGEVLELVLENFVYPWYRDITDDEACVDEVRQTFRFFASVLVRRAQKVDVPSVATGKMMKAAMKHIEIVAKAQQKVRNSEGLQQAALEEYGADLHVALRSRRDELNYLRKLTEMLFPYVMPPKATDCRSLALLIREVMTGSVFLPTMDFMADPDTVNHMVLIFIDDTPPEPATEPPSTLVPFLQKYADSRNKKPSVLKLDLKEIREQQDLLFRFMSFLKQEGALHVLQFCLTVEEFNDKILCPELSDSEMEQLHDEVQTIYKTYCLEESIDKISFDPFIIEEIRSIAEGPYTGVVKLQQMCCLFEAYEHVLSLLENVFTPLFCHSDEYFRYLLCGAESPARNAKLNRNLSLDDLSSDSTPPGWADNIISHRPLSINTKPPPPSGSPSIKSILTLRPRILKSLPTSTKNTSKRGESFGISRIGSKLKGVFRSTTMEGAMLPAYTMVDGDEDMVEEATVVVEDESPVPLDVVSNLGTQRNLSAWTIAIPFVDFYEDEAKKERVPVFCIDVERHDRQHASHELETWSIYRRYLEFYVLESKLTEFHGSFQDAQLPSKRIIGPKNYEFLNSKRGEFEEYLQNLLRHPELSNSQLLADFLSPYGMETQFLDKMLPDVNLGRIFKSVPGKLIKEKGQNLEPFLQSFFGSCESPKPKPSRPELTVLSPTAGNKKLLNELYKNNANQPDSMDRRQNQNYFMEVMDLEGVYDYMMYVGRMVFRMPDWLHHVLCGGRILFKRTLEAYMDNYLQSKLDKILQEHRVVSLITLLRDAVFCENNEERSPEDKQRRAKQTFEEMMNYLPDIVGKCIGEENKSEGIQLLFSCIQQPLLNKQMTYVLLDIAVQELFPELHSSTKEDPSSRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DY20</accession>
    <name>A0A3B4DY20_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tripartite motif containing 65</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRIM65</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017556588.1">
        <property type="nucleotide sequence ID" value="XM_017701099.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017556596.1">
        <property type="nucleotide sequence ID" value="XM_017701107.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DY20"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027899"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001354.2">
        <property type="protein sequence ID" value="ENSPNAP00000027899.1"/>
        <property type="gene ID" value="ENSPNAG00000013469.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429392"/>
    <dbReference type="CTD" id="201292"/>
    <dbReference type="GeneTree" id="ENSGT00940000154294"/>
    <dbReference type="OMA" id="ACTVNEC"/>
    <dbReference type="OrthoDB" id="5951542at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19835">
        <property type="entry name" value="Bbox2_TRIM65_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="326"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="211"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="58230" checksum="28A935E11986D02E" modified="2018-12-05" version="1">MEAHNLSCAICLERFKFPVTIPCGHTFCKDCISRHWDQREKVGPQPAEFNCPVCNETFSSRPTLKRNVSLSLLTEVTVNSSAGKDNSASCRGDAANAGPEQFCGRHQKPLVLYCRNDGMCVCCACAVNECKSHDKVLIEEERNSREAGLKKKGLEVKKCREDTERNILELSENIAKAKVSLQQTSQWVNVKFTQLLKVLVEKQELTQSFVEQQQESALSQAQARLSELQEKAQQLLELEEQIDSLCALPDCLLIQDSRLVEVPQMGEVPVEVSVNVQDKLNPVTDLLSRVSKLVCEDLDKAVYAVVGHDKEGSPQDKRPVLAVVPSPATPSYPAGKEGLSAYRCSLTFDPRTANAHLLLSQSNRRAEHLTSGPRPVPAHEARFDHTWQVLCFENFTHGRHYWELEVSKPWAYVGVTYQAIPRKEKGKTCMLGMNELSWSLQLDERQLSAWHAGRRESVAGQLQLHTQPLRIGMLLDYEAGTLTYYGEGQVRLHAFHCVFSQELFPACWIGEGVSVTLCPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0Y5</accession>
    <name>A0A3B4E0Y5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Beta-galactoside alpha-2,6-sialyltransferase 1</fullName>
            <ecNumber evidence="16">2.4.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ST6Gal I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sialyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ST6GAL1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a beta-D-galactoside + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;6)-beta-D-galactosyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28034"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:136398"/>
            <dbReference type="EC" id="2.4.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.1" evidence="16"/>
    <dbReference type="RefSeq" id="XP_017554127.1">
        <property type="nucleotide sequence ID" value="XM_017698638.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0Y5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028909"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015516.2">
        <property type="protein sequence ID" value="ENSPNAP00000028909.1"/>
        <property type="gene ID" value="ENSPNAG00000003244.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427995"/>
    <dbReference type="CTD" id="6480"/>
    <dbReference type="GeneTree" id="ENSGT00940000157053"/>
    <dbReference type="OMA" id="PFSALPW"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003835">
        <property type="term" value="F:beta-galactoside alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097503">
        <property type="term" value="P:sialylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23985">
        <property type="entry name" value="GT29_ST6GAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000012">
        <property type="entry name" value="ST6 beta-galactoside alpha-2,6-sialyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46059">
        <property type="entry name" value="BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46059:SF2">
        <property type="entry name" value="BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="502" mass="56425" checksum="DD1438BCE961CEA1" modified="2018-12-05" version="1">MGYKIPGAAMDRVSLIWRLRRRARRGALCMALFCMTMAVLYTLCAENSVPVTDAIFGVRARTRAQPRTHSIVKVLRGVGAKTIYVDPQKLPGVIPGDPKKPIPVLSSSTYAMEGSHKDHPFRRRDREPHGLLSWLLGAPLRRALETILGGRRRADAGGLKGDAEFFGPNGALGEIWDDEMSSTMLGKRLRKVVQNYQAMNKYGVQLSGSERATRRHKLSGPELLCQLKEMVQITTLTPEMKPFAGFSWASLLPQRPLTSDLGPFKTCAVVSSAGSLRNSGLGKEIDSHDTVVRFNAAPTAGFEKDVGSKTTVRLINSQVMASEDHHFLSSSLYSTGILVAWDPAPYSSDLNEWYNKTDYPIFKQYQRYRRLHPQQPFYILHPSVEWQLWRRVQDNMGEPIQKNPPSSGLLGTVLMMSLCDVVHVYEFLPSRRKTELCHYYQRFSDAACTLGAYHPLLYEKNLVKRMNQGSDRDIYTHGRVTLPGFSTFNCTHTFTSSTFSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1V3</accession>
    <name>A0A3B4E1V3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit A</fullName>
            <shortName evidence="8">eIF3a</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">eIF-3-theta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EIF3A</name>
        <name evidence="8" type="synonym">EIF3S10</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="7">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eIF-3 subunit A family.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000029214"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016106.2">
        <property type="protein sequence ID" value="ENSPNAP00000029214.2"/>
        <property type="gene ID" value="ENSPNAG00000015241.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111063"/>
    <dbReference type="OrthoDB" id="18884at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.860:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.860:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.860">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03000">
        <property type="entry name" value="eIF3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027512">
        <property type="entry name" value="EIF3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054711">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22591">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="initiator methionine" description="Removed" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="10">
        <location>
            <begin position="315"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="794"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="558"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="667"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="794"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="844"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1135"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1153"/>
            <end position="1285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029214.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1296" mass="154453" checksum="CCA134F502F9EC32" modified="2024-11-27" version="2">MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVIKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKLAEEKTETAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSKVERLYHDIAQQAFRFCLQYTRKAEFRKLCDNLRMHLGQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFALSKKPPKPQLMANYYNKVSTVFWKSGNALFHACTLHRLYHLSREMRKNLTQEEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKHRRLATLLGLQSPPTRQSLINDMVRFNLLQYVVPEVKELYSWLEVDFHPLKLCGRVTKVLSWVREQAEKEADLQQYVPHLQNNTILRLLQQVAQIYQSIEFSRLASLVPFVDAFQLERSIVDAARHCDLQVRIDHTSRTLSFGSDLNYSTKEDSPVGPFLQNMPSEQIRNQLTAMSSSLAKAIQVIKPASILQEREEHHQQAIAAYLKNARKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERIMQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKKAYEEQRVKDMELWELQEEERISNMKVEREKALEHKKRLSRMVEDKENFVSKITAARSFIYEEKLKQFQERLVEERKKRLEERKKQRKEEARNAFYRQKEEEAQRIREEQLKKEREERERQEQELREEEEREYQERLRKLEEQERKQRARQQEIEERERRKEEERKAPEEKGSKDWGEKDEGGWRRRGEGESDWRRPVADNREWRQEGREDNDREEGEPPFRRGGDGPRRGGADDRGLRRGFEDERGPRRSGDDDRGPRRGFDDDRGPRRGLDEDRGPRRGLDEDRGPRRGLDEDRGPRRGLDEDRGPRRGLDEDRGPRRGLDEDRGPRRGLDEDRGPRRGLDDDRGPRRGLDDDRGPRRGFDDDRAPRRGMDEPRGPRRGADDDWGPRRGGDDERGGRRGMDDSASRRSDDPKPWKPSGRPGGWREREKAREESWGPPRESQGHDDEGDHEEDRNEGDRYRDRRPPREEGGWRRGGTDEPSSWRDSRREDFDRDERRDRRDMKDRRDDRDRDRDRGPPRDHDDGGSWRRGGDDRREERKDDRDAPPRPRERDGERSSWRTDKEKDNPRRTKNETDDDGWTTVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1Y5</accession>
    <name>A0A3B4E1Y5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1Y5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017068.2">
        <property type="protein sequence ID" value="ENSPNAP00000029703.2"/>
        <property type="gene ID" value="ENSPNAG00000016001.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154967"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051503">
        <property type="entry name" value="ComplSys_Reg/VirEntry_Med"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785:SF2">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="82"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="227"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="146"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029703.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="309" mass="34910" checksum="5955E064BF5EEF45" modified="2024-11-27" version="2">ELFSNLVAVIIFQDQPVLPVSYCKRPPLRNGFLVPWQHIYDHGTKVSYGCNKGLKPVVETWWGELLCGNGEWSHTPHCVPESNCIAPVVPHATPKPSQVSYPNNSIVTFVCDKGYEFELQLQEKQAACENGEWGPLPKCQRKRNACDAPSHVPNALIKHPYRDTFDEGETITYLCKEGYEFEGKDKSTCAKSTWDHTPKCGKTVLLLLSSLYGTSKVNANVSPFSVTECGAYPSVKNGKLDISDFALTFRCYPPYELRGPQQVMCLRGQWSEVPVCVGQRHLFLTQMNVPNEFIFTSYNRHACCSFFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E755</accession>
    <name>A0A3B4E755_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMTC family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017542295.1">
        <property type="nucleotide sequence ID" value="XM_017686806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E755"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032447"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022623.2">
        <property type="protein sequence ID" value="ENSPNAP00000032447.1"/>
        <property type="gene ID" value="ENSPNAG00000020375.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414015"/>
    <dbReference type="CTD" id="555381"/>
    <dbReference type="GeneTree" id="ENSGT00940000156144"/>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000130">
        <property type="entry name" value="Transmembrane and tetratricopeptide repeat containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013618">
        <property type="entry name" value="TMTC_DUF1736"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052384">
        <property type="entry name" value="TMTC_O-mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44216">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44216:SF3">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08409">
        <property type="entry name" value="TMTC_DUF1736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="9 13" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017376800" description="dolichyl-phosphate-mannose--protein mannosyltransferase" evidence="16">
        <location>
            <begin position="21"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="396"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1736" evidence="17">
        <location>
            <begin position="250"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="496"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="530"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="609"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="646"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="680"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="782"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="336"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="839" mass="94146" checksum="236728427C9DB3A2" modified="2018-12-05" version="1">MIADLVCSAVALVLYVNTLGADFCYDDSRAIKTNQDLLPETPWINIFYDDFWGTLLTHSGSHKSYRPLCTLSFRLNYALGSMEPWGYHLVNVGLHCIVTALFSSLAGLLLGRGCWRLLAGLLFASHPVHTEAVAGIVGRADVGAALFFLLALRCYIGHCTLRAEGAYGQSWAWFLGSLACSACSMLWKEQGVTVLAVAAVYDVFVFHRMTLRQLPGIFCKKKNLHLLLSVFLLGSWGVFLLACRLYWMGNKPPNFSNSDNPAADCPCFLTRVLTFFFLPAMNLWLLLCPDMLSFDWSMDALPLLRSLTDCRNLHTVVFYAGLLALAWFGLCSRPSKEKETNGKTHHVTNGKSSTNGHSYHSDYEHSIPDSNSVQTNGNGTKKHYLTRTSLPTTENVVVFSLGLLAIPFLPATNLFFYVGFVVAERVLYIPSMGFCLLVAVGVRALWVRLRRSSSRTLVLACSGALVLLFGVKTVLRNQDWHNEEMLYKSGITVNPAKAWGNLGNVLKNQGKMAEAERAYRNALYYRRNMADMLYNLGLLLQENNKFSEALHYYKLAIGSRPTLASAYLNTGIILMNQGRLDEAKRTFLTCADIPDENLKDPHAHKSSVTSCLYNLGKLLHEQGHQEEALSVYKEAIQKMPRQFAPQSLYNMMGEAYMRLNKLSEAEHWYRESLRAKPDHIPAHLTYGKLLAMTGQKTEAEKYFLKAIQLDPTKGNCYMHYGQFLLEESRLLEAAEMAEKAAQLDSEEFDVVFSAAHMLRQASLNDAAEKYYEQAASLRPNYPAALMNLGAILHLNGKLQEAEANYLRALQLKPDDTITQSNLRKLWNIMEKQGLRTRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7J5</accession>
    <name>A0A3B4E7J5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc phosphodiesterase ELAC protein 2</fullName>
            <ecNumber evidence="6">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">ElaC homolog protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Ribonuclease Z 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">tRNA 3 endonuclease 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">tRNase Z 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">ELAC2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Zinc phosphodiesterase, which displays mitochondrial tRNA 3'-processing endonuclease activity. Involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="24">Homodimer. Interacts with PTCD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion matrix</location>
            <location evidence="4">Mitochondrion nucleoid</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017563592.1">
        <property type="nucleotide sequence ID" value="XM_017708103.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7J5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031271"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020102.2">
        <property type="protein sequence ID" value="ENSPNAP00000031271.1"/>
        <property type="gene ID" value="ENSPNAG00000018524.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433494"/>
    <dbReference type="CTD" id="60528"/>
    <dbReference type="GeneTree" id="ENSGT00730000111191"/>
    <dbReference type="OMA" id="INYICQL"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000014">
        <property type="entry name" value="Zinc phosphodiesterase ELAC protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNase Z endonuclease" evidence="26">
        <location>
            <begin position="87"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="215"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="800"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="800"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00046098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF13691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="845" mass="94722" checksum="20CBADF42C49F091" modified="2018-12-05" version="1">MLAALRRSRATAWQFLKRATPLPDLLGHGDVNAGVSRAACLRPWSSSMLPRAGKKPARDAGLRHVRSKEKRAHRELPGPSTVHAQAVSAGSRESGSALYVFSEYNRYLFNCGEGTQRLMQEHKLKAARLDNIFLTRMSWETVGGLSGMILTLKDTGVPECVLSGPPQLEKYLNAIKVFSGPLEDIKLSVRPYTDSVYKDDTMTVTQVPIFAEQKAVGMNSSPTSSQRRELWRPDDCEESQTNNRATSPEGKERTTRDPSLVVAYVCKLHPKKGNFLVQKAKELGLPVGTAAIGPIIAALKDGKSVTYEGKEIQPEEVCTPTDPGPVFLVIDCPSVQFIQPLCSNEVLQRYQSGEDGATLVVHMTPESVLKTEEYQCWIERFPSSTEHMIMNEQACCIHNVRSQKIQTQLNLIHTDIFPQLQHYKSTESQAALHVPNVRAECLLKFQLRPKAEWQRDAIPPCDEEEFVKEAAEVPNFLQEVEKFKQFQMTDSVALSGKVDRYPEVVFLGTGSALPMKIRNVSGTLVNISSSQSLLMDCGEGTFGQMCRHYGNHVDEMLTKLSTVFISHIHADHHTGLVNLLLQRERALNSLGKAFTPVYLVAPTQIMTWLNQYHDHCQEILSHVNFIPAKCLCEGAEMPKFKTKSFIQAVLKRNDLTKFQTCLVRHCKNAFACSLTHQSGWRIVFSGDTMPCDALVHMGKNATLLIHEATLEDGMEEEAMDKRHSTTSQAIDIGMKMNAEFIMLNHFSQRYAKIPLFSDDFNQRVGISFDHMRIRPGDFQILPRLLAPLKALFAEEIEEMEERRERRELRAGKTLGVESNGEAGGGAKREQEEPNQDMASKRLKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7N2</accession>
    <name>A0A3B4E7N2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sphingomyelin synthase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = an N-acylsphing-4-enine 1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:36079"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:73203"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:36080"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539614.1">
        <property type="nucleotide sequence ID" value="XM_017684125.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017539616.1">
        <property type="nucleotide sequence ID" value="XM_017684127.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7N2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031780"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021017.2">
        <property type="protein sequence ID" value="ENSPNAP00000031780.1"/>
        <property type="gene ID" value="ENSPNAG00000019300.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412193"/>
    <dbReference type="CTD" id="445247"/>
    <dbReference type="GeneTree" id="ENSGT00940000157370"/>
    <dbReference type="OMA" id="CERSDYG"/>
    <dbReference type="OrthoDB" id="422827at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006686">
        <property type="term" value="P:sphingomyelin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF24">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE:CERAMIDE CHOLINEPHOSPHOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin synthase-like" evidence="12">
        <location>
            <begin position="234"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="406" mass="47191" checksum="B062867EB6450C5D" modified="2018-12-05" version="1">MAVSQLPDSGEHSNNSSYQHVEVTVTDSPHMQTTSSRLTHPSQDMQDNGQQGNGRSSRLSRGFAKVLRKNQDYIRIRVPESRLDQLPPEWWKTGLSFMWAGFNLFCTTVMITVVNDRVPDKSVNPPLPDKFFDYVPRVEWAFTITEVNGMILVMAWFVQWLLLKHKSIIGRRFFFLMGTLYLYRIITMYITTLPVPSMHMNCAPKLYDDPIGKVHRVLQLLSGGGLSITGSHMMCGDFLYSGHTVMLTLTFLFIREYSPRSLWWYHLICWFLSAVGVVCILLAHEHYSVDVIVAYFITTRLFWWYHTLANNQSLRDSPHNYFNRVWWRFFFRFMERNVTTTVPCTFSWPLPLPATCSKNPCRRYSIVQSVREEADGEKNRFLLCERSDYGCSQISDSAVEAEVCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7Z5</accession>
    <name>A0A3B4E7Z5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Nitric oxide synthase-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NOSIP</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Negatively regulates nitric oxide production by inducing nitric oxide synthase translocation to actin cytoskeleton and inhibiting its enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the NOSIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7Z5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031416"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020367.2">
        <property type="protein sequence ID" value="ENSPNAP00000031416.1"/>
        <property type="gene ID" value="ENSPNAG00000018761.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427363"/>
    <dbReference type="GeneTree" id="ENSGT00390000015505"/>
    <dbReference type="OMA" id="PCVTKFM"/>
    <dbReference type="OrthoDB" id="116827at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16661">
        <property type="entry name" value="RING-Ubox1_NOSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16662">
        <property type="entry name" value="RING-Ubox2_NOSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000251">
        <property type="entry name" value="Nitric oxide synthase-interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001144">
        <property type="entry name" value="Nitric oxide synthase-interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016818">
        <property type="entry name" value="NOSIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031790">
        <property type="entry name" value="Znf-NOSIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13063">
        <property type="entry name" value="ENOS INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13063:SF10">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15906">
        <property type="entry name" value="zf-NOSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023577">
        <property type="entry name" value="ENOS_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Nitric oxide synthase-interacting protein zinc-finger" evidence="13">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="125"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR023577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="308" mass="33439" checksum="BBDAF715CC73BD9A" modified="2018-12-05" version="1">MTRHGKNCTAGAVYTYHEKKKDTAASGYGTQRVRLGKDAVKDFDCCCLSLQPCKDPVVTKDGYLYEREAILEYILHQKTEIAKKMKAFEKQKQALKSEGQLESCSEEREKVEKFKKREGSIVSKPINPFISGHSKEMASPNGSVSSSVSSSSSASGTSVSSSSSSLPSFWIPSLTPEAKPTLLKKPSKTVKCPMSGQSLKMSDLITVRFTPLDASLDRVALLTRQDRYVCGVTKDTLGNSVPCAVLRPSGSVVTLECVEKLIRKDMIDPVSGDKLKENDIIVLQRGGTGFAASGVDLCAKEARPVMQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8D0</accession>
    <name>A0A3B4E8D0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein 63, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MRPL57</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8D0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031514"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020554.2">
        <property type="protein sequence ID" value="ENSPNAP00000031514.1"/>
        <property type="gene ID" value="ENSPNAG00000018898.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008171"/>
    <dbReference type="OMA" id="QEFGHAA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016576">
        <property type="entry name" value="Ribosomal_mL63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14520:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14520">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14978">
        <property type="entry name" value="MRP-63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="12849" checksum="5D96242784BD7A30" modified="2018-12-05" version="1">MMFLTLALFRKGIPGRQWIGKHRRPRPITWQMKRNVLKHLEREAENEFWISRPYMSMEQERGHAAARREHIWTNIREERRAKFPQHKYVTEHLDQLNVTRTWNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8U9</accession>
    <name>A0A3B4E8U9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HSD3B7</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552896.1">
        <property type="nucleotide sequence ID" value="XM_017697407.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8U9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031699"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036882.2">
        <property type="protein sequence ID" value="ENSPNAP00000031699.1"/>
        <property type="gene ID" value="ENSPNAG00000019186.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427348"/>
    <dbReference type="CTD" id="80270"/>
    <dbReference type="GeneTree" id="ENSGT00940000160236"/>
    <dbReference type="OMA" id="GDHFKRG"/>
    <dbReference type="OrthoDB" id="10262413at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000220">
        <property type="entry name" value="3 beta-hydroxysteroid dehydrogenase/Delta 5--&gt;4-isomerase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF847">
        <property type="entry name" value="3 BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="365" mass="41643" checksum="BEA0DA90246B101F" modified="2018-12-05" version="1">MATEKLVYVITGGCGFLGQHLLNVLLEKEENIKEIRLFDKLADPSLQTLSTERVKVVVIQGDITDYQNVMEACKGADLIIHAASLVDVWHRIPEKTIYAVNVGGTKNVINACVELGIQYLIYTSSMEVVGPNVKGDTFIRGDEDTPYKVYHDMPYPKSKAEAETLVLEANGKKVNGGESLYTCALRPTGIYGENHQLMKDFYQMGVRTGGWLIRGVPRDTEHGRVYAGNVAWMHVLAARAIKEHPETIGGEVYFCYDDSPYKSYEDFNMQFLSAFNFRSLRVPLWLLWFQACMNDMLHWVLRPIYNFTPLLNRYTLAVACTSFTVSSDKALRHFQYRPLYDWEQCRARTQKWVDTFPLETSSKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFL3</accession>
    <name>A0A3B4EFL3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Sterol regulatory element-binding protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Sterol regulatory element-binding transcription factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">SREBF2</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">COPII-coated vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the SREBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538682.1">
        <property type="nucleotide sequence ID" value="XM_017683193.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFL3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034084"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038333.2">
        <property type="protein sequence ID" value="ENSPNAP00000034084.1"/>
        <property type="gene ID" value="ENSPNAG00000023124.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411562"/>
    <dbReference type="CTD" id="6721"/>
    <dbReference type="GeneTree" id="ENSGT00940000157339"/>
    <dbReference type="OMA" id="QQAVMIT"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010886">
        <property type="term" value="P:positive regulation of cholesterol storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18922">
        <property type="entry name" value="bHLHzip_SREBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000016">
        <property type="entry name" value="Sterol regulatory element-binding transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062:SF3">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="19" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BHLH" evidence="25">
        <location>
            <begin position="357"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="443"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00039750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00042214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1144" mass="125266" checksum="E10664939BD10C9A" modified="2018-12-05" version="1">MDAGEFLMDNMDPTLSELGDEFTLGDIDEMLQFVSNQADFPDLFEDQVGSVPLQAGVGSNIRPSAAGLQSNPVTLMPATPTPALPLTPPHTPVQTITGQQTRVPPLLQPRPQPQQHIQVAAAPVPLQTQTVTVQSQSFPVQTLAVQAHTQTLPVQTQAQTVMIAPTAAQSRFIQNPVICHQNPNTSFQVLQPQMQSIVTSPQVQPMTIQHQRLLTPPCQTIQTLSTAPTAVHTVSSQVQQVQQVQQVPVLVHQPQILKTDSLVLTTLKHDGTQVLSTMQNPTGITTLTTPLQTALQVPTLVGSNILTTVPVVMGGAEKLPIKQLQSGPTHSGGITRTVVEQSTAVTTATGGVVKEGERRTTHNIIEKRYRSSINDKILELRDLVMGSDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENLALKMASQKHRVICVADDVEMKPDVCVMTPPPSDSGSSSPPQSSPFCLDSEPGSPLLEHEQVKSEPASPSSSVGVMDRSRVLLCALTFLCLSLNPLPSLLGSDDNGAPSWPGGEGHGPSRSLVWLPAQTQSFTAWLWCVLPWVTVWVLSGVGVVWGCVRILYLWEPVTPLHSPTSVLFWRHRKQADLQLFRGDYSAAVVNLETCLSVLSRALPSTNFDLACSLSWNLIRYCLHRPAPFGWLVRLVGGKHEGEESQTSSRDAALVYHRLGQLQLTGKMSERSALWGVCVCLNAVNLSESARGKMNSTEHAQIYISAAISLRTALGNHLTCLPGYLLSCAESLIGHSDSKPLPDSLRWLFTPLGRQFFLSCDWSVKPESSGGVYTSEREQADPIAQLHRCFCEKLLERAVHTLIQPHTDTETSKHREENGEFSGVLEFLQLLNSCTEDSASPTPPFPALANHNMTPVGDPVCRWWASILKASVHWLQGDDVSVRLMLAEVERMPRALHSIDHPLSKAVLALCKAVQMSVCPQKGEGTLSCITHCDRASAYLHSSISHTHTYTWLHKGMELLMCDLLLTLRTSLWQRGGGANGEPGPAPSSQLAGFQRDLSSLRKLAQGFPQAQHKVFLHETTVRLMAGASPTRTHQLLEHSLRRRTHTYTEGVCVAGERERAHAILLAFSHLPVPLLTPPGHRARMLAEAKRTLERAGDRRTLTDCQHILLRGTTITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EI02</accession>
    <name>A0A3B4EI02_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Autophagy related 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATG2A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Preautophagosomal structure membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATG2 family.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000036127"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039744.2">
        <property type="protein sequence ID" value="ENSPNAP00000036127.2"/>
        <property type="gene ID" value="ENSPNAG00000026827.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00620000087966"/>
    <dbReference type="OMA" id="RPCAQIH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026849">
        <property type="entry name" value="ATG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190">
        <property type="entry name" value="AUTOPHAGY-RELATED 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13190:SF21">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 2 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13329">
        <property type="entry name" value="ATG2_CAD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="677"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1000"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1197"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1208"/>
            <end position="1225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036127.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1659" mass="184526" checksum="66094AC739BFA0C4" modified="2024-11-27" version="2">MSRWFFPWSGSIKKRACRYLLQHYLGHFLEERLSLEQLSLDLYNGSGVIKEINLDVWAVNELLESLGAPLEIVEGFVQSIAVTIPWAALVTDHCTLEVTGLQITCRPKSWDSQGWSSCMTSSMQLAQECLKDPPEASEDPPAPLEGLEMFAQTIETVLRRIKVTFLDTIVRIEHQPFDSETGVALEMRITRLDYCDEAVRDSNQTVPVDIHQPPAFLHKILQLNSVKLLYETLGEPQPLLIGSCSGFMETTVKIKQNDMLPGPKVCGLTYAWRSSHGGQAESMKPDSLFRLSLGGVTLTLLEQEPPLGPYGQSSLAEVSRVFFHELAFFKDSMFSERNFQNLRGNFAKACPHSHLRLTGAAVQVACEMRSSGRHARSVMSDLSFSRLELLECLWEPGNPQYIECFPTDLQSGLKQRVVTIYLLVLVVFINDLCTFQLLYLHLFSFICPPSISPQTQSLEVHSCLTLLSPHAMLRLRFPIPDLRPLDQRRPISKRAVRKEILSLEMSNLEMKSQQGPDIQETPSTPSAKLIQLFEATFSDMHGVYEGWEGGSFPCIRVQKSTDWQNPDEAGTDSRGVVGVGLNRDSGLGSVSMENPCELREKNSSPFSSNRTMFETEEMVIPADPEEMKEFQSQCVAQCQYVVDITLPVAYILFPSKEAFQSIYNRINNDLLMWEPPLSPPSPPKSFHSPSQQHRSEDDEFQLCKSAFRLGQVEQCHGEMLLDFEGGKIFSVTQHQNNPNLNFLTIESKKVELYHKAEVQDSPIPVRLEMPRFSPPNNLDPTIYPTEVGVSSVGGREMGGQMLSTAIKITLDLQRNVKEFLVALRLQGATLQHHMALTNHSWHEQLVDFLDVIDDDILGYTPPAIITVLHTHLATCAVDYRPLYLPLRVLFTAESFSLSSNIIVDTATFHLRFILDDSALYLTDKCESETVDLRRDYVCVLDIDLLELAITTWKGNDTGRLTQPLFELRCSNNVVHVRTCADSCAALVNMLQYLVSQGDLHPPPRHASPTEIAGQKLPPSEAAAPLPPCPPAETAEINQCDLTDALIDTERTMREEDQDTGEILIRGSPVSVYLFPGEAPKPRSAMLEGDVSELDRLVVTATEAHADMMSEEGSEGSTDNDDFCILEAPGMGIPPRDGEPVVTVLCQGPIKVRDGYFSRPRGSSDLLRAPSRFPVPQSRVVLREVSIVWHLYGGRDFGGKPTSAHTQHTPTGARSSPSRSAGSSRPQNSWRWVGGSGRQHSLLMEIQLTKVEYDSEGAGVGEQPLSRQVFIVQELEVRDRLASSQINKFLYLYTSESMPRRAHSNMLTVKALQVLPEAGLGGPECCLRVSLLPLRLNIDQDALFFLKDFFSNLAAGVNPYLPVDPSEEAESVSGLGPDLTASVETTYSEQSSSSAGSSSSSDQPIYFREFRFTSEVPIWLDYHGKNLNTSHSHLSSSVRLLGVDKVIQYAVNEWLTDIRKNQLPGILGGVGPMHSVVQLFHGVRDLFWMPIEQYRRDGRIIRGLQRGAASFGTSTASAALELSNRLVQAIQATAETVYDILSPTPPLTRYITEGRPTSRPRRTPQPADLREGVAKAYDTVREGVIDTAQTLCDVASRGHEQKGLPGAVGGVLRQIPPTVVRPLIVASEATSNLLGGMRNQIKPDARKEDFLKWRTEDGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EI18</accession>
    <name>A0A3B4EI18_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EI18"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034856"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027106.2">
        <property type="protein sequence ID" value="ENSPNAP00000034856.2"/>
        <property type="gene ID" value="ENSPNAG00000024399.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271828"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07698">
        <property type="entry name" value="IgC1_MHC_I_alpha3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043792234" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="195"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034856.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="338" mass="38387" checksum="24CDBFA1D1055676" modified="2024-11-27" version="2">MRGTAFLLLLLSAVSSAVTAGTHTLHIAFSIQTGISSLPEGMSSMLFDKEYIAHYNSDKMLIPIQNWVKEALGDVLWNNFTSFLSSQIEHLLHGVREAEQLVWDKGVHTLQRRLSCQLNISGVPEVSDEYGYDGETFISLDQEHKYVADMPDAETIVRRWSRHGKEKHIARHCIDWLKRIQNHGKNRTRRPVSPPEVSFLQKTPSSPVVCFATGFYPRAVNMSWQRNGEDVSQNVRVGETLPNDDGTFQKRVSLNVTPEEWNENQYSCVVQHNETIKNLTEEIKTNYGPQPRNQAIVCIPVVLVASVLFILRIALSKTLQDECECLVRCPVDRKLMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJT9</accession>
    <name>A0A3B4EJT9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017559370.1">
        <property type="nucleotide sequence ID" value="XM_017703881.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559372.1">
        <property type="nucleotide sequence ID" value="XM_017703883.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJT9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035554"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028742.2">
        <property type="protein sequence ID" value="ENSPNAP00000035554.1"/>
        <property type="gene ID" value="ENSPNAG00000025628.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431018"/>
    <dbReference type="CTD" id="777631"/>
    <dbReference type="GeneTree" id="ENSGT00940000158322"/>
    <dbReference type="OMA" id="GLNHGCL"/>
    <dbReference type="OrthoDB" id="2588702at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="456"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="544" mass="61283" checksum="DD357252B38FAA43" modified="2018-12-05" version="1">MVTVRRLLGLNAEAGSEDIRAFFHGLHIPEGGVHIIGGRRGEAFIIFTTEKDGQLAMERNGELLKGSSITLHVSSLAEFKRKIESRLRKQKRSVVEKKAAQMERKKPDVKKVVEKADLQVAALPELGTALLLGIMAAFQGIQSNNQESQSQSLPASTHQLPASPRPHQSGNQMIQGMDQTHHINKGKESIAQPMRVHTLEHESSSREIKAHEPGYLRLYGLPNHVTKTEVGRVLEGLSVLEVIPSVFLGRDYCCLVKVASLEEAEEGLKYSHRTLEDSFVEIRMAHERMWTYATELYECGQCSTSSEPDRISPDGHLFQTSLYKKVSEDRSLSGSPKRYRFDSPPPTTEYCVMVKNLSSKTTKTEIKELFSCFHLPNNKIIHLLNKWGERTSTAFIIFTHAEDYASAMNMDGSPNGSKTLVVSSITMDKMRELMYKERHKETKRSYFPEKDWTAPSCIYARNFPADVRKIEVKDFFCLYRISEDDITLLKDGNGNGTGEALIEFNCEQAAKEAHSLHGDVFLGQKILLTCISPQQMRSILQETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK12</accession>
    <name>A0A3B4EK12_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK12"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036235"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039861.2">
        <property type="protein sequence ID" value="ENSPNAP00000036235.2"/>
        <property type="gene ID" value="ENSPNAG00000026913.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009571"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF61">
        <property type="entry name" value="INACTIVE SERINE PROTEASE 37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="20"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036235.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="245" mass="27965" checksum="149027952D02D67B" modified="2024-11-27" version="2">LVPFFKKMLTMILLIKKRVYVGHNAPGGNASNNEGQHHVILELHGCPGEVLCGGSLIHQDWVITAAHCDCPGLKVFVNAHPNRTKEEERGIDDRQILRDNNTYHDLMLLKLKKGNRENLTTIHLPPEKCNAPPANTEVRFYGWKDAKLNNQITNISSSTHSILHCNTASFWRQRSFMCFDFNMVVIQKLLLLLYLQGDSGGSLVWNKTLYGVLRGGSPYLNRSPRYFMDICYPLYRNWIKQNTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKI0</accession>
    <name>A0A3B4EKI0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ZNF341</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017578509.1">
        <property type="nucleotide sequence ID" value="XM_017723020.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EKI0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036400"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030879.2">
        <property type="protein sequence ID" value="ENSPNAP00000036400.2"/>
        <property type="gene ID" value="ENSPNAG00000027229.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442806"/>
    <dbReference type="CTD" id="84905"/>
    <dbReference type="GeneTree" id="ENSGT00390000000546"/>
    <dbReference type="OMA" id="DGPCAML"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000679">
        <property type="entry name" value="Zinc finger protein 341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000867">
        <property type="entry name" value="Zinc finger protein 341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001132">
        <property type="entry name" value="Zinc finger protein 341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003238">
        <property type="entry name" value="Zinc finger protein 341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000611">
        <property type="entry name" value="zinc finger protein 341 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000618">
        <property type="entry name" value="zinc finger protein 341 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001031">
        <property type="entry name" value="zinc finger protein 341 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF139">
        <property type="entry name" value="PAIR-RULE PROTEIN ODD-PAIRED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="455"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="485"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="522"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="604"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="632"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="153"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="374"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="714"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="722"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="751"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036400.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="826" mass="91492" checksum="7A82483C3D991C08" modified="2024-11-27" version="2">MSQAIFEVLEGMDNQTVLAVQSLLDGQGGVPDPGTQTVSGTTIQSMDDDDVFLCGKCKKQFNSLPAFMTHKREQCQPNTPSLATVSLASSNAYTSISAVPQPPVNRQVSTYITVPPSPLTHTLVQGNVLVSDEVLMSAISAFTSMDQPMAAMQASTQSNLSMPTGASYLQHHPQPPHPLPPSQTQSLSSQIPSSNNSSVVQVYSALPPMAGGGNAEVHALGLQSFQPVQGPSQCVESQSQAFSSAPAYSLAKPTTKAKTCANTAALPELGEYDKVIIPKKARNTKKGQDVGQTKAKGQKLKCNFCDKVFTKNFDLQQHIRSHTGEKPFQCIVCGRAFAQKSNVKKHMQTHKVWPAGVHSTVSRLPITVKVVPVNTDENTPQPAEAQEAQTAVEQSEEELQAEAARASEAKGPSQGHSKQIILIDSSYQCQFCAAKFSTYFQLKSHMTQHKDEQVYRCVVKTCSQTFQKLDLFLEHIRTHQEHLTYRCHLCCKVFPSLFELGLHQYSHSFCPQQNPRKEANYYRCNKCQSKYSTQEALEQHLLTASHNYPCPHCQKVFPCERYFRRHLSTHGAGGKFKCQICKKFFKTEHYLKLHTQIHSGEKPYKCSVCEATFNRKDKVKRHMLIHEPFKKYKCPFRTHVGCTKEFNRPDKLKAHILSHSGIKPFKCQLCQKSFSRRAHMLEHQRSHTNNYRFRCSTCSKGFSRHKYYRDHKCPLAGAPAEGAERRSRGRSAEGSKGTDPEPAAEGPDTPETPEEEEDEEEEEDEDDGNDEGETSVSGVLETEMGERGEEEERDEGLVAGASLEQMQDGGGADGLLAAPVFGAAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKV4</accession>
    <name>A0A3B4EKV4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-type tyrosine-protein phosphatase kappa</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">PTPRM</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035894"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039524.2">
        <property type="protein sequence ID" value="ENSPNAP00000035894.1"/>
        <property type="gene ID" value="ENSPNAG00000026591.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155020"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14633">
        <property type="entry name" value="R-PTPc-M-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14635">
        <property type="entry name" value="R-PTPc-M-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000003">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000005">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000019">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000006">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase T isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000009">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000048">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045911">
        <property type="entry name" value="R-PTP-mu_cat_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF11">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE, RECEPTOR TYPE, M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017442319" description="Receptor-type tyrosine-protein phosphatase kappa" evidence="20">
        <location>
            <begin position="21"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="23">
        <location>
            <begin position="25"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="187"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="287"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="385"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="483"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="21">
        <location>
            <begin position="953"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="1127"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="21">
        <location>
            <begin position="1239"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="1415"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1505" mass="170185" checksum="80EEE17378693AE8" modified="2018-12-05" version="1">MDGVLPGLLALVTFLASVRGQVFRGDCNFERPYTECGYSQGKDDDFDWEQVNTKQRPSTDPWIPAGSAFMMVNTSGKFLQQKAQLFVPQLKENDTHCLTFQYYGASGEGSSPGHLNIYVKENNSPLGLPVWNSSGPAFHTWRQVELAVSTFWPNFYQLIFEAVSSGQPGLLAIKDISLQGHQCMSTPHFMHIKGVEVNARQTATFHCTINGQPKDHLNYKLWLQGIGGREAPMKDTKPVNLRRYMATFDVENTTKGDSGRYRCIVQSDKGVGVSSYAELIVKQPPVPIAPPQLTAVGATYLWIQLNANSINGDGPIIEKEVEYRTMSGSLIDSQPVDKPNHKIGHLDPDTEYEISVLLTRPNEGGTGAPGPPLKAKTKCADPMHGPRRLEVVDIKSRQITVRWEPFGYNVTRCHRYNLTVEYRCKVNRKDETKEEEIDSHNGVSQYTIRNLSPYTNVSVKLVLRNPEGVKESQELAVLTDEDVPGAVPLESIQGSTYEEKIALKWREPIQTYGIIKQYEIVYKAVSSFDPEFNLTNQSGKVLKPSNETSHTFVGLYPGSTYSFTIRASTVKGYGSQAITQFTTKISPPHMPGYDQETPLNQTDSTVTVLLKPAQSRGAPVSVYQVVVEEERPRRSQRTAEILRCYPIPIHFQNATVLSSRYYFTAEFPASRIQSSLPFTIGDNKTYEGYWNAPLLPHKSYSIYYQAVSKANGETKIDCVRVATKAAIIVTQLTTPYIRIVPAAGDSQLTGAATRKPDAVEPEKQTDHTVKIAGGIAGILLFVIIFLGVVLVMKKRRTYHSYTYYLKLAKKRKETLSSTRQEMTVMVNSMDKSYTEQGTNCDEAISFMDTHNLNGRTVSSPSSFTMKTNTLSTSVPNTYYPDPFVPTAILVPINDENHTVTSDTSSLVQSHTHSYKKREAVDVPYQTGQLHPAIRVADLLQHITQMKCAEGYGFKEEYESFFEGQAAPWDSAKKDENRMKNRYGNIIAYDHSRVRLQALDGEQSSDYINANYIDGYHRPNHYIATQGPMQETVYDFWRMVWQENTATIVMVTNLVEVGRVKCCKYWPDDTEIYRDMKVTLIETQLLSEYVIRTFAVEKRGAHEIREIRQFHFTGWPDHGVPYHATGLLGFIRRVKSKSPANAGPMVVHCSAGAGRTGCFIVIDIMLDMAEREGVVDIYNCVRELRSRRVNMVQTEEQYVFIHDAILEACLCGDTTIPANQLRSVYYDMNRLDPQTNSSQIKEEFRTLNMVTPTLRVEDCSIALLPRNHEKNRCMDVLPPDRCLPFLITIDGESSNYINAALMDSYKQPSAFIVTQHPLPNTVKDFWRLVLDYHCTSIVMLNDVDPAQLCPQYWPENGVHRHGPIQVEFVSADLEEDIISRIFRIYNAARPQDGYRMVQQFQFLGWPMYRDTPVSKRSFLKLIRQVDKWQEEYDGGEGRTVVHCLNGGGRSGTFCAISIVCEMLRHQRSVDVFHAVKTLRNNKPNMVDLLDQYKFCYEVALEYLNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELF4</accession>
    <name>A0A3B4ELF4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017562497.1">
        <property type="nucleotide sequence ID" value="XM_017707008.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELF4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036613"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031429.2">
        <property type="protein sequence ID" value="ENSPNAP00000036613.1"/>
        <property type="gene ID" value="ENSPNAG00000027581.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432878"/>
    <dbReference type="CTD" id="160622"/>
    <dbReference type="GeneTree" id="ENSGT00530000063734"/>
    <dbReference type="OMA" id="YQTCIYQ"/>
    <dbReference type="OrthoDB" id="10041077at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043523">
        <property type="term" value="P:regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900141">
        <property type="term" value="P:regulation of oligodendrocyte apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021510">
        <property type="term" value="P:spinal cord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06713">
        <property type="entry name" value="PDZ_tamalin_CYTIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000157">
        <property type="entry name" value="General receptor for phosphoinositides 1-associated scaffold protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052122">
        <property type="entry name" value="Intracell_Traff_Signaling_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15963">
        <property type="entry name" value="GENERAL RECEPTOR FOR PHOSPHOINOSITIDES 1-ASSOCIATED SCAFFOLD PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15963:SF3">
        <property type="entry name" value="PROTEIN TAMALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="91"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="329"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="390" mass="43021" checksum="3D736D8A8301D0C8" modified="2018-12-05" version="1">MKNMTFRRIKKLNSNEPDGGSGGSCPGSGDIYFSSSKSDSCKTMDPSGSSDIYNYKTLAYSGGTLPRNFKKNGGLQKWKPLTQPPEPERKVVVLEKKEDETFGFEIQTYGLHHQDENSVEMCTFVCKVHEDSPAKLAGLKVGDTIACVNEISVEGFRHKDIVQLVRSSGNTIRLETVYSDTIRKAELEARLQYLKQTLHEKWDEYRSLMVQEQRLVHGIVMSDVAVYESLESAGIYGSLGAPSPVVMRALRCTGTGSANSSISHLSSATGDDDPLYQTCFYQAENGSSNDTSSNPGEACMERPQLGRQRLLRPASELFASAKTSLTRSASTRSYLKGSSSSSSSAPSGEKQQCGAFSSLQRKPKQKSFRRRLLKYIPGLNRPLEEEESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ENQ9</accession>
    <name>A0A3B4ENQ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017559628.1">
        <property type="nucleotide sequence ID" value="XM_017704139.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ENQ9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037570"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034058.2">
        <property type="protein sequence ID" value="ENSPNAP00000037570.1"/>
        <property type="gene ID" value="ENSPNAG00000004585.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431156"/>
    <dbReference type="CTD" id="100334391"/>
    <dbReference type="GeneTree" id="ENSGT00940000164792"/>
    <dbReference type="OMA" id="DSNIMVL"/>
    <dbReference type="OrthoDB" id="9449045at2759"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006178">
        <property type="term" value="P:guanine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000038">
        <property type="entry name" value="Hypoxanthine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050408">
        <property type="entry name" value="HGPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="6">
        <location>
            <begin position="51"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="225" mass="25281" checksum="12633F21CD86E066" modified="2018-12-05" version="1">MTHSLVGSGRKEQGVVIEEGWPGYDLELFNYPEHYRGDIDSVLIPHGVIMNRIECLARDILEEIGHHTLMVLCVLKGGYKFCADLVDSIKAQSHSTNSKLTTRVEFIRLKSYLNDHSTEDLHIIGPEDLSMLKGKNVLIVEAIVDTGKTMKALLQHVEMFQPKAVKVAGLLVKRVPNGPGTLPDYVGFVIPNRFVVGYALDYNEYFRDLNHICVISETGKLKYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EPJ6</accession>
    <name>A0A3B4EPJ6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PTPRO</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017578472.1">
        <property type="nucleotide sequence ID" value="XM_017722983.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017578473.1">
        <property type="nucleotide sequence ID" value="XM_017722984.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EPJ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037106"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032666.2">
        <property type="protein sequence ID" value="ENSPNAP00000037106.1"/>
        <property type="gene ID" value="ENSPNAG00000006837.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442786"/>
    <dbReference type="CTD" id="5800"/>
    <dbReference type="GeneTree" id="ENSGT00940000154814"/>
    <dbReference type="OrthoDB" id="8609993at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072112">
        <property type="term" value="P:podocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003093">
        <property type="term" value="P:regulation of glomerular filtration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd14614">
        <property type="entry name" value="R-PTPc-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000039">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase O isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058859">
        <property type="entry name" value="Fn3_R-PTP-O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042996">
        <property type="entry name" value="PTPRO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47028">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47028:SF1">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26586">
        <property type="entry name" value="Fn3_R-PTP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017208039" evidence="9">
        <location>
            <begin position="21"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="843"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="903"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="348"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="454"/>
            <end position="550"/>
        </location>
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        <location>
            <begin position="551"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="703"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="748"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="10">
        <location>
            <begin position="961"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="11">
        <location>
            <begin position="1135"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
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    <sequence length="1239" mass="139299" checksum="918118BE57947A90" modified="2018-12-05" version="1">MTAALPCVLSLLLSIQTVAPFQATLRDDNFILITLEPSDMRENVTAYAAKISGEPHSHILPFQSANRSQVASATLLYNASYHGLCYTISLLTGNASTWSRPDKSVALLTKPLPLDSVQILDYRAAPETGVVFELSSPERNTFSRVNISYFEGREQRSMLYKDFLKGKTVFKHWLPGTCYSNITFQLISEATVNKSTLVKKSEVTHEPRHHRTVPNPPLNVSLKIVHLSGRAHESPLSRRIRDIPLIEEQEEMDIREREQEPDPEGTNAVTQQPHSINSSLAPVTSTTAHTTTEEDVSFNFSNTSADENATDASWPEPTGSVSPTDDEFVNAVVPEYEDSNEPGSAMDLTTEPTIMPTRLPPILLELRWLPPRPPTSFDGFNIYIYRDGNSTETATVDENTHEFFAELSEPGTYRVLVTTISSSGDCEPRESAPHTGFTFYLSPTGEWLQQPTERPQAVSVKMLGTSTASVSWAPATQSHNGSLVSVLSLTCLKPSPSQRMENTYCSEENTTSDIITNLTPGAQYRVVVYHTNGPLVSPPSEPVIIDIEPTGVRDLVVYPLSPTAVILSWQRPYNVAFRKYVLQTFFFNSATQTSQWTTYYEIAATASVIASVRITDLLPAWYYNFRVTMVTWGDPPLSCCDGSTLSFITAPEAPHISSVDYSHGVLYVRWTYGDLFTDLSHSRMLHWQVVAEGKKGSKRRFSTDVTRNMMKATLALPPGDIYNLTVTACTERSHNTSTPHIMKLEPAPPKSLYAVNATHSAVTLLWTEEGVVDFCQISCKQLGPNKQLKAVEPQTALSHVLTVSNLQPSTSYNCSVTSYSYSTPSEPAYITISTLAQEMNPSVAAISALAVLSVLLISLLVLFLLVLRKKHMQMTRECGAETFVNFASFERDGKLPYNWRRSLFAFLTLLPSCLWTDYLLAFYINPWSKTALKKRKLTSPVQLDDFEAYVKDMSKDSAYKFSLQFEELKSVGLDLSHDAADLPVNRPKNRYTNILPYDFSRVKLISMHNDEGSDYINANYIPGYKSPREYIATQGPLPETRNDFWKMVLQQNSHVIVMLTQCNERRRVKCDHYWPFTDEPVSYGEITVEMLSESESPEWTIRNFRLGYADETQDVLHFNYTSWPDHGVPTVNAIESILQFVQIVRQQVNRTKGPVIVHCSAGVGRTGTFIALDRLMQHIREHEYADILGMVSEMRSHRLSMVQTEEQYVFIHQCVLLMWKKKKQQSLSSDVIYENVTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQ81</accession>
    <name>A0A3B4EQ81_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase C and casein kinase substrate in neurons 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PACSIN2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017549447.1">
        <property type="nucleotide sequence ID" value="XM_017693958.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017549448.1">
        <property type="nucleotide sequence ID" value="XM_017693959.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037394125.1">
        <property type="nucleotide sequence ID" value="XM_037538228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EQ81"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034860.2">
        <property type="protein sequence ID" value="ENSPNAP00000037933.1"/>
        <property type="gene ID" value="ENSPNAG00000030040.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425364"/>
    <dbReference type="CTD" id="11252"/>
    <dbReference type="GeneTree" id="ENSGT00950000182973"/>
    <dbReference type="OMA" id="AMAHVFK"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11843">
        <property type="entry name" value="SH3_PACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000014">
        <property type="entry name" value="Kinase C and casein kinase substrate in neurons protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000205">
        <property type="entry name" value="Protein kinase C and casein kinase substrate in neurons protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF14">
        <property type="entry name" value="PROTEIN KINASE C AND CASEIN KINASE SUBSTRATE IN NEURONS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="10"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="424"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="312"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="55743" checksum="9F78783A4D97FC51" modified="2018-12-05" version="1">MSGTYNDSLIDVSSDSFWEVGNYKRTVKRVDDGNRLCNDLMNCIHERARIEKGYALQLTEWAKRWRQLIEKGPQYGTVERAWCAMMTEAEKVSELHMEVKAALMGEDFEKVKNWQKDAYHKQMIGGFKETKEAEDGFRKAQKPWAKKLKEVDTMKKAYHAACKEEKLATSRENNSKLESNANPEAQKKLQDKVEKCQQEVQKSKERYEKSLEELDKVTPQYMENMEQVFEQWQQFEDKRLRFFKELILDIKQHLDLSTNHKFQTVYQNLEDTIQAADAEEDLKWFRSNHGPGMPMNWPQFEDWSVDLNRTLSRREKKKPSDEVTLTGINQTVEQTTKTNNSLTVSSSTEPVGSNPFDDDDDEEETPAEQPNSSPVNAKKEEVISKTASTVEKTQDWSDEETGNPFAADANGNGNPFDDEPASPAVSVPVRALYDYEGQEQDELSFKAGDEFTKIGEEDEQGWCKGRLKDGHVGLYPANYVEDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IK40</accession>
    <name>A0AAR2IK40_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017567161.2">
        <property type="nucleotide sequence ID" value="XM_017711672.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IK40"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077782.1">
        <property type="protein sequence ID" value="ENSPNAP00000038369.1"/>
        <property type="gene ID" value="ENSPNAG00000032920.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108435675"/>
    <dbReference type="GeneTree" id="ENSGT00940000161337"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000096">
        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF998">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="28"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="307" mass="35130" checksum="29FD9FC5B976B2DD" modified="2024-11-27" version="1">MNSSRDPFEEAFMKNLITVGLGVIITYINGIFVIAFLKNPVFYTDTRYILYIHLVINDIIMLFLSVTLHVLSYALPFLNVTVCSFLLLFATTTAGNTPLNLAGMALERYIAICKPLHHPQLCTVKRTYILICLIWILTFIPALTDIIIVLVNEPISIFSTSIICYSKSLFSTNQHFVRAVFIDALYLSLVWITLIFTYLKILQAAKAATSDQVSARKARNTILLHGLQLLLCMLAYVTPLLNYFFFALFPKHRTIFMYLGYLVSNVIPRLLSPLIYGARDQKMFRYVRMYFTCNLFNTKVKPKANEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ILA3</accession>
    <name>A0AAR2ILA3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="9">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2ILA3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053656.1">
        <property type="protein sequence ID" value="ENSPNAP00000038789.1"/>
        <property type="gene ID" value="ENSPNAG00000002776.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234646"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.20:FF:000001">
        <property type="entry name" value="[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000007">
        <property type="entry name" value="Mitochondrial pyruvate dehydrogenase kinase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF21">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE ISOZYME 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="241"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="410" mass="46817" checksum="FF1BA56F87B20DE9" modified="2024-11-27" version="1">MKCSYMLLNLLLKDITPKIEYYSRFSPSPMSIKQFLDFGEENACEKTAYMFLKKELAVRLANTMREVTLLPGNLLSQPSVQLVQSWYSQSFEELLECENRSPEDPRTLNDFLDILIQIRNRHNDVVPTMAQGVIEYKEKFGFDPFISSNVQYFLDRFYTSRISFRMLINQHTLLFGDGINPAPPKHIGGIDPNCNVVEVVRDAYETAKMLCEQYYTRAPELQIKEFNSKKPNKPIQVVYVPSHLFHMLFELFKNSMRATVELHENSLEGLPPVKALVTLGKEDLSIKISDQGGGVPLRKIDRLFNYMYSTAPTPRLNSSQTAAPLAGFGYGLPISRLYARYFQGDLNLYSMEGVGTHAVIYLKALSSESFERLPVFNKSAWRHYQLGPEADDWSNPSSDPCDASTFKVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IMS8</accession>
    <name>A0AAR2IMS8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="10">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">G6PD</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:15842"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IMS8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048006.1">
        <property type="protein sequence ID" value="ENSPNAP00000039314.1"/>
        <property type="gene ID" value="ENSPNAG00000020429.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063435"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000013">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 10" id="KW-0521">NADP</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="95"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="268"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="571" mass="65368" checksum="B27B8328C8EFD65B" modified="2024-11-27" version="1">MVQRSPFKVERGNSKKKAGDQVNLLAEYTWARCTYDVVFVSILRLAKNTIKYPFLTPLTETMNTLPLSRSEVFGELRKELYEDEEFHQTDVHIFIIMGASGDLAKKKIYPTLWWLFRDGLLPEQTYFVGFARSDLTVDAIRTACLPHMKVSEPPLSFFSRNSYISGKYGDEAAFSNLHSHLLSLPGGADANRLFYLALPPTVYHDVTKNIRHHCMSTKGWNRVIVEKPFGHDLQSSEELSNHLSSLFTEDQIYRIDHYLGKEMVQNLMVLRFGNRIFGPIWNRDSVACVVLTFKEPFGTQGRGGYFDDFGIIRDVMQNHLLQMLCLVAMEKPASTSSDDVRDEKVKVLKCIAPVSPSDVVLGQYVGDPEGEGEAKLGYLDDPTVPKASTQATFATAVLYVRNERWDGVPFILRCGKALNERKAEVRLQFTDVPGDIFSGQCRRNELVVRVQPNEAIYAKMMSKKPGVYFSPEETELDLTYRSRYKDVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIEKEKIPPIKYKYGSRGPSEADELVQKVGFRYEGTYKWVNPHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IS18</accession>
    <name>A0AAR2IS18_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RNASEH2B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IS18"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057206.1">
        <property type="protein sequence ID" value="ENSPNAP00000040834.1"/>
        <property type="gene ID" value="ENSPNAG00000035453.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263500"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000003">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051320">
        <property type="entry name" value="Viral_Replic_Matur_Polypro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064">
        <property type="entry name" value="POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064:SF37">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="312" mass="35408" checksum="F239D0FDA13CD8C5" modified="2024-11-27" version="1">MAQGITNAPSTFQRLMEKCVGDMNLREVVVFLDDVIVFSKTLEEHEIRLMRVLSRLKEYGLKLSPAKCRFFQTSVRYLGHIVSQSGVETDPDKVEALKTWPRPQNLKQLRSFLGFSGYYRRFIKDYSSIVKPLTDLTSGYPPLRKSFKHKAKDSQYHDPKESFGGRWTSACETAFNTIITKLTTAPVLGFADSKIPYVLHTDASTTGLGAALYQEQEGIMRVIAYASRGLSRSESKYPAHKLEFLALKWAVTEKFNDYLYGSNFTVITDSNPLTYILTTAKMDATSYRWLAALSTFTFKLKYRAGKQNSDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IVS5</accession>
    <name>A0AAR2IVS5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538541.1">
        <property type="nucleotide sequence ID" value="XM_017683052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IVS5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077202.1">
        <property type="protein sequence ID" value="ENSPNAP00000041706.1"/>
        <property type="gene ID" value="ENSPNAG00000035326.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411476"/>
    <dbReference type="GeneTree" id="ENSGT01030000234555"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001609">
        <property type="term" value="F:G protein-coupled adenosine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001634">
        <property type="entry name" value="Adenosn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF54">
        <property type="entry name" value="ADENOSINE RECEPTOR A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00424">
        <property type="entry name" value="ADENOSINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="23"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU201114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="327" mass="36229" checksum="415CC05F729623CF" modified="2024-11-27" version="1">MADVGEVIYTALEVFIAVACCLGNVLVIWAVWVSGALRQPTFCFIASLAVADFLVGALAVPLAVLVDGWVEMSFQGCLFISCVVIVLTQASVYSLLAIAVDRYLRVYIPLKYKGLAKRTHSWAIVAVCWMIASVLGFIPMFGWNNYNHLQPSNSTTIVCQFLTVIPMSYLVNFIFLSCLLPPMIIMTVLYGFIFCTISQQLRGGVIRASKSGAYYVKEKKLASSLALVLALFAACWLPIHIMNTMEFYNQSSLVPHIAFYVGILLSHANSAVNPVVYAFKIPKIKRAYKMAWRKFLPCRREQQTDQMAENYASSITYSTAKSAAGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IW51</accession>
    <name>A0AAR2IW51_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">GRIK5</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15 20">Postsynaptic cell membrane</location>
            <topology evidence="15 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Presynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037393069.1">
        <property type="nucleotide sequence ID" value="XM_037537172.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393070.1">
        <property type="nucleotide sequence ID" value="XM_037537173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IW51"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083271.1">
        <property type="protein sequence ID" value="ENSPNAP00000041836.1"/>
        <property type="gene ID" value="ENSPNAG00000020554.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432328"/>
    <dbReference type="GeneTree" id="ENSGT00940000158852"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06394">
        <property type="entry name" value="PBP1_iGluR_Kainate_KA1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000060">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000072">
        <property type="entry name" value="glutamate receptor ionotropic, kainate 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000059">
        <property type="entry name" value="Glutamate receptor, ionotropic, kainate 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000010">
        <property type="entry name" value="Putative glutamate receptor ionotropic kainate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 20" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6 20" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 20" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043112511" description="Glutamate receptor" evidence="20">
        <location>
            <begin position="31"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="638"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="818"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="22">
        <location>
            <begin position="430"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="23">
        <location>
            <begin position="440"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="915"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="988"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="920"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="992"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="688"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="736"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="18">
        <location>
            <position position="666"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="748"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1046" mass="116055" checksum="9C208B6CF8744351" modified="2024-11-27" version="1">MPELPALLLLSIHFHILLVTVVTMPPSALSQPSVLSSVRMAAILDDRSACGRGERLALALARENINSVMEGPARARVDVDVFELQKDSQYETTDTMCQILPKGVVSVIGPASSPASGSTVSHICGEKEIPHVKIGPEETPRLPYLRFASVTLYPSNEDLSLAVGAILRSFSYPSASLVCAKAECLLRLEELVRRFLISRETLSVRMLDDDLDPTSLLKEIRDDKIATIIIDANASVSYLILKKASELGMTSAFYKYILTTMDFPLLWLDDIVDEHSNIVGFSMFNTTHPFYLEFIRSLNLSWREGCDINPYPGPALSSALLFDAVHVVASAVQELNRSQEIGVKPLSCTSPQIWQHGTSLMNYLRMVEYDGLTGHVEFNSKGQRTNYTLHILEKHKTGHKQIGIWFSNNTLVMNSTSLDINVSETLANKTLIVTTILENPYVMRKVNYQELEGNEQYEGFCVDMLRELSDILKFTYRIKLVDDGLYGAPEPNGSWTGMVGELINRKADLAVAAFTITSEREKVIDFSKPFMTLGISILYRVHISRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRERRDMLENQYTLGNSLWFPVGGFMQQGSEIMPKALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEAPIESPDDLADQTNIQYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSKYAFLLESTMNEYHRSLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEISLAVLQLQESNRLEILKRRWWEGGQCPKEEDHRAKGLGMENIGGIFVVLICGLIIAVFVAVMEFVWSTRRTAETDEVSVCQEMLTEFRNAVSCKKSSRLRRRRPLSSSLALRHPARVALGAPRPLRLVREMRLSNGKLYSGAGPLTGGASGAGAGPSELGPGPQRLLEDPLGASSTPTVPLGPPAVTVPLPRGCTHVRICQECRRIQSLRTTSCPRVPPSSAPLPRLAPPPPHSSSNTDSEGGGSHSPRRIGHSPPPRGGTSTDLLGEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZK0</accession>
    <name>A0AAR2IZK0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hypermethylated in cancer 2 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CCDC85A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional repressor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZK0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042491.1">
        <property type="protein sequence ID" value="ENSPNAP00000043482.1"/>
        <property type="gene ID" value="ENSPNAG00000037891.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159978"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000195">
        <property type="entry name" value="Hypermethylated in cancer 1 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000536">
        <property type="entry name" value="hypermethylated in cancer 2 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000746">
        <property type="entry name" value="hypermethylated in cancer 2 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000032">
        <property type="entry name" value="hypermethylated in cancer 2 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF22">
        <property type="entry name" value="HYPERMETHYLATED IN CANCER 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="16">
        <location>
            <begin position="73"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="503"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="255"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="330"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="529" mass="59413" checksum="941C028921ED51B6" modified="2024-11-27" version="1">MCCGGLTFSNKIMSFCLFRQTVRMHHLFLETDTFPAHESHLNEKHQGQEMELSNYASQLLLQLNQQRSKGFLCDVVIMVENTLFRAHKSVLAATSSYFKSLVLHDNLIHLDSELVDPDVFQTILDFIYTGRLSDESQGTERVWNLSTLLTVANYLQLHDLASLCSSKLEPSGSATGKSSLRTHQPFSPTSKTLPKREAEDTSSDIEAYASMVPPKKRNNGENKCISNNKQSFGLDLTKKSTPFCSVVSLSNEVSKGYSQSSVSRATANSLPSGVEEKRSPEKASGLVSFNGAQEWRRTPPRERSRRKGNVNGYFPVIKSGSHISQNLVFKSESPDDKESHCEENGRDQPENGESSDTSPNFVYRQESYLKQPRGDYPYVCIPCGKGFPSSESLNSHVESHIDVDMEGTKEAEEGDEDPAVTVLQEAPGTTKKKPLKSHKELDALRPFPCNICGKMFTQRGTMTRHMRSHLGLKPFACEECGMRFTRQYRLTEHMRVHSGEKPYECQICGGKFTQQRNLISHMRMHTSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J6L8</accession>
    <name>A0AAR2J6L8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR2</fullName>
            <ecNumber evidence="4">3.1.3.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Myotubularin-related protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphatidylinositol-3-phosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">MTMR2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Lipid phosphatase that specifically dephosphorylates the D-3 position of phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate, generating phosphatidylinositol and phosphatidylinositol 5-phosphate. Regulates the level of these phosphoinositides critical for various biological processes including autophagy initiation and autophagosome maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1-D-myo-inositol-3-phosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42328"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65221"/>
            <dbReference type="ChEBI" id="CHEBI:78934"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:42329"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:45632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78911"/>
            <dbReference type="ChEBI" id="CHEBI:85342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:45633"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:39019"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="EC" id="3.1.3.95"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:39020"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:12317"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.95" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J6L8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048412.1">
        <property type="protein sequence ID" value="ENSPNAP00000047580.1"/>
        <property type="gene ID" value="ENSPNAG00000003095.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153669"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000038">
        <property type="entry name" value="Myotubularin 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF42">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="23">
        <location>
            <begin position="200"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="381"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="613"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="617"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="19">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="325"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="350"/>
            <end position="351"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="412"/>
            <end position="418"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="638" mass="72467" checksum="9A7549C104D17DB1" modified="2024-11-27" version="1">MISRLVIVVIEGLVIDRLVISGLVIKGLVIEGLVISGLVIDGFGDQQVGDRWVEDQQYQCSCSCQWNPDLEPGALIFISCLSVGKDVTYICPFVGALRGTLTVTNFRLFFRGISRDPAFVLDLPLGVVSRVERISGASSRGDVSYVLVCKDVRTLRFVHKQPDDSLQKSVFEVLTKFAFPVSNSLPLFALEYNQVFPENGWKVYDALAEYKRQGLPNESWRISKVNDHYELCDSYPAALVVPVTITDDELRRVAAFRAKGRIPVLSWLHPESQAAVVRCSQPMSGVGGKRCKDDEKLLQAIMDANAQSHKLFIFDARPSVNAAANKMKGGGCESEDAYQNAELVFLDIHNIHVMRESLRKLKEVVYPNIEESHWLSNLESTHWLEHIKLILAGALRIADKVESGKTSVVVHCSDGWDRTAQLTSLSLIMLDSHYRTISGFQKLIEKEWISFGHRFQQRVGHGDQNHTDADRSPIFLQFIDCVWQMTQQFPAAFEFNESFLVTVLDHLYSCLFGTFLYNSEQQRVKEELAKRSVSLWSFVNSQVDEFINPLYVHYPAHVLLPSVSIRHLQLWVSYYIRWNPRVRPQQPVHQRYKELLAKRAELQKKVEELQREVHNRASASSERAGSPTRSITPVQTFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J747</accession>
    <name>A0AAR2J747_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">LIM homeobox transcription factor 1, alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LMX1A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J747"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067136.1">
        <property type="protein sequence ID" value="ENSPNAP00000046054.1"/>
        <property type="gene ID" value="ENSPNAG00000008180.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166054"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000095">
        <property type="entry name" value="LIM homeobox transcription factor 1 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000006">
        <property type="entry name" value="LIM homeobox transcription factor 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF88">
        <property type="entry name" value="LIM HOMEOBOX TRANSCRIPTION FACTOR 1-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 12 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 13" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="38"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="98"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="17">
        <location>
            <begin position="195"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="197"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="156"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="41423" checksum="A7DB0647BF247032" modified="2024-11-27" version="1">ERREAQKKTDRRFISLQSSEHHGGGQLMCVDPSLQAVCVCAGCARPIRERFLLRVHCGVWHERCARCCECGELLEKSCFSRDNKLYCRTDYQRLFLVRCRACAEVISPAELVMRAGSAVFHLRCFCCSVCSCRLQKGDRCVLVGDRLLCTRDYQHSLSSPATSDSGRSEDEDDDDEESSLKAAGERNAVGDGELKRPKRPRTILTTQQRRAFKASFEVSSKPCRKVRETLAAETGLSVRVVQVWFQNQRAKMKKLARRQQQQQQQSPSCGLPVELKSMDLYVSVAHQQQQAVSLESHSFKLDPFRQGLTPPQMPGDHMNPYGCEAFYNDVDDSPLCHLGDCMSSEETSLLTPIDRLYSMHDSYFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J8D3</accession>
    <name>A0AAR2J8D3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Integrator complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">INTS3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Integrator subunit 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566891.1">
        <property type="nucleotide sequence ID" value="XM_017711402.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J8D3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061971.1">
        <property type="protein sequence ID" value="ENSPNAP00000048200.1"/>
        <property type="gene ID" value="ENSPNAG00000027555.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435513"/>
    <dbReference type="CTD" id="65123"/>
    <dbReference type="GeneTree" id="ENSGT00390000014184"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056518">
        <property type="entry name" value="HEAT_Ints3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045334">
        <property type="entry name" value="INTS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019333">
        <property type="entry name" value="INTS3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587:SF7">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24566">
        <property type="entry name" value="HEAT_Ints3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10189">
        <property type="entry name" value="Ints3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 3 N-terminal" evidence="7">
        <location>
            <begin position="63"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Ints3-like C-terminal" evidence="8">
        <location>
            <begin position="560"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="953"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="985"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1020" mass="116549" checksum="817EDBA167EDEB65" modified="2024-11-27" version="1">MEPSAAKGSRTQGRLLAATSLDAKDELEERLERCVAITTSITNGLSEREANDALTAHVCKGPQQHEEVCLGLFTLLLTEPPQAQRCYRDLTLVNRDGMNVVLIKINQILMEKFLKLQDVCRTQLVWLVRELVKSGVIGADGVLMTLMKQIAGGDITSKNLWLAENVLDILVEQREWVLKSGMLVAMSVYTYLRLIVDHGTPSLLPLRQREVDFCVGLLRERFMECFIIGRDLVRLLQNVARIPEMELLWRDLLHNPQALSPQFTGLLQLLTSRTSRKFLACRLTPDMETKLLFMTSRVRFGQQKRYQDWFQRQYLSTAESQSLRCDLIRYICGVVHPSNEVLSSDILPRWAIIGWLLTTCTSNVAASNAKLALFYDWLFFNPEKDSIMNIEPAILVMHHSMKPHPAITATLLDFMCRIIPHFFPPLEVQVRQGVFNSLNFIMEKRVLAHLAPLFDNPKLDRELRSMLRERFPEFCSSPSPPTEVKMEESVPMEMDNHVLDKEDGCYDNTDATFSDDEEELNNKGKKREFRFHQIKETFIDEPADITPYVDQLDETLKEKVLQLQKSSDTETQCEVMQEIVDLILEEDFESEQMSALASCLSELFKSHFRGDVLPEEISEESLEESVCKPVCLIFRNLCQMQEDNSGFSVLLDMLAELYQKQPKIGYHLLYYLKASKAAAGKMSLYESFAQATALGDLHTCLMMDMKACQEDDVRLLCYLTPSIYSEFPDETLRSGELLNMIVAVIDSTQLQELMCHVMMGNLVMFRKDSVLNILIQSLDWETFEQYSTWQLFLAHSIPLETIIPILQHLKYKEHPEALSCLLLQLRRENPSEEMVKMVLSRPCHQEDQFTTSILRHWAAKHDDLLAEHIKALLIKNNNMPRKRQSLRSSSSKLAQLTLEQMLEHLDCLRLSLNNTKNNFFSQTPILQALQHVQASCDEAHKMRFSDLFSLAEEYEDSSKPPKSRRKAPASSPRSRKGAAAPPANNEDDSASSSASEEEDSKPKPPKRKRKGSSAVGSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JBS7</accession>
    <name>A0AAR2JBS7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Interacts with ALKBH4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JBS7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054820.1">
        <property type="protein sequence ID" value="ENSPNAP00000049473.1"/>
        <property type="gene ID" value="ENSPNAG00000007814.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074807"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000020">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="11">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="259" mass="29054" checksum="AD9D816ABB364E69" modified="2024-11-27" version="1">MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVVVTQKKVPDKLLDASTVTHLFRITENIGCVMSGMTADSRSQVQRARYEAANWTYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMIVIGIDEENGPQVYKCDPAGYYCGFKATAAGVKQTEATSFLEKKVKKKHDWTFEETIETAISCLSTVLSIDFKPSELEVGIITAEDPRFRTDQYLLNMRHSGYNFPQLCSSIWTHLDISFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JF90</accession>
    <name>A0AAR2JF90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4E family member 1c</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JF90"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061058.1">
        <property type="protein sequence ID" value="ENSPNAP00000048992.1"/>
        <property type="gene ID" value="ENSPNAG00000027050.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166192"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000006">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E family member 1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF29">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E FAMILY MEMBER 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24794" checksum="8BBB2F9663174301" modified="2024-11-27" version="1">GATEEFEQQRGTESEEASNDSPTPAVASPEQYIKHPLQNRWALWYFKNDKSKSWTENLRLISKFDTVEDFWALYNHIQQPSKLGFGCDYCLFKDGIKPMWEDDRNKLGGRWLMTLSKQQRHNDLDRYWMETLLCLIGESFDEASDDVCGAVVNVRPKGDKIAIWTGNCQNREAIMTIGQQYKERLSLPIKMVIGYQSHDDTSSKSGSTTKNMYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JG42</accession>
    <name>A0AAR2JG42_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Creatine kinase S-type, mitochondrial</fullName>
            <ecNumber evidence="3">2.7.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Sarcomeric mitochondrial creatine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CKMT2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15 17">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JG42"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052679.1">
        <property type="protein sequence ID" value="ENSPNAP00000051003.1"/>
        <property type="gene ID" value="ENSPNAG00000006567.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182772"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000002">
        <property type="entry name" value="creatine kinase S-type, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000002">
        <property type="entry name" value="Creatine kinase S-type, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF19">
        <property type="entry name" value="CREATINE KINASE S-TYPE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="18">
        <location>
            <begin position="66"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="19">
        <location>
            <begin position="179"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="182"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="346"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="374"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="440" mass="49457" checksum="90CA85BB35A46419" modified="2024-11-27" version="1">GLECHCGSCGWWSLLLPAKTWLMASTFTRMLSGRNAAVLMASLGAGTLGAGYLMSETGVSAAERKKLYPPSADYPDLRKHNNCMASSLTPAIYGRLRDKETPNGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEESYEMFAELFDPVIKDRHNGYDPKIMKHPTDLDSSKITSGMFDDKYVLSSRVRTGRSIRGLSLPPACTRSERREVERVVVQALSGLKGDLSGKYYSLTDMTEREQQQLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNNEKTFLIWINEEDHTRVISMEKGGNMKRVFERFCRGLKDVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVRLPLLSKDPRFSKILDNLRLQKRGTGGVDTAAVGDTFDISNLDRLGKSEVELVQCVIDGVNFLIECEKRLEKGQDIKHILKNPLRTHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JK16</accession>
    <name>A0AAR2JK16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Connector enhancer of kinase suppressor of Ras 2b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNKSR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JK16"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062711.1">
        <property type="protein sequence ID" value="ENSPNAP00000050534.1"/>
        <property type="gene ID" value="ENSPNAG00000001903.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156709"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06748">
        <property type="entry name" value="PDZ_CNK1_2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01260">
        <property type="entry name" value="PH_CNK_mammalian-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09511">
        <property type="entry name" value="SAM_CNK1_2_3-suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000019">
        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000092">
        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000060">
        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049628">
        <property type="entry name" value="CNK1-3_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010599">
        <property type="entry name" value="CNK2/3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051566">
        <property type="entry name" value="CNKSR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017874">
        <property type="entry name" value="CRIC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844">
        <property type="entry name" value="CONNECTOR ENCHANCER OF KINASE SUPPRESSOR OF RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844:SF21">
        <property type="entry name" value="CONNECTOR ENHANCER OF KINASE SUPPRESSOR OF RAS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06663">
        <property type="entry name" value="CNK2_3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10534">
        <property type="entry name" value="CRIC_ras_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51290">
        <property type="entry name" value="CRIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="11"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CRIC" evidence="7">
        <location>
            <begin position="84"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="215"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="569"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="319"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="477"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="680"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="831"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="995"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="701"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="750"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="870"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1034" mass="116686" checksum="B690F2B67B5AAA2B" modified="2024-11-27" version="1">MALVMEPVSKWSPSQVVDWMKGLDDCLQQYIKNFEREKVGGDQLLRITHQELEDLGVSRIGHQELILEAVDLLCALNYGLETENLKTLSHKLNASAKNLQNFITGRRRSGHYDGRTTRKLPNDFLTSVVDLIAAAKSLLAWLDRSPFAAVTDYSVTRNNVIQLCLELTTIVQQDCTVYETENKILHVCKTLSGVCDHIISLSSDPLVSQSAHLEVVQLANIKPTEGLGMYIKSTYDGLHVITGTTEGSPADRCKKIHAGDEVIQVNHQTVVGWQLKNLVNSLRGDPGGVTLTLKKRPQSTLTSAPALLKNMRWKPLALQPVAPKSPTSSSATPTSTISTPSKRDSSALQDLYIPPPPSEPYTPRDEQGNLPGDDRGQGGIPAAKGSESPNSFLDQEYRRRFPLMEEEAVLYCYEYDPNRGGPPSRRESTPTYGRLRPISMPVEYNWVPDYEDPARLKREGRRENSMLRYVSEEKVPEEYLTSRSAKRDTGKRTKKKSEKGGSPAHYSLLPALQMEVLRQESMSTPSSDSTSLYHTFQGSSLLSKSKKKSKGGPLSSISKRRISCKDLGRGDCEGWLWKKKDAKSYFSQKWKKYWFVLKDACLYWYMNEEDEKAEGFVSLPEFKIDRASECRKKYAFKACHPKIKSFYFAADGVDDMNRWLSRLNMAAAGYAEREKIRQEQADYWSESDHEDNETPSTPKQDSPPPPYDTYPRPPSMTCASPYLEPKHGRLSSTETSQSRSSHEEYRSEPPGGGSSSGGGSPARKSASQRRSWQDLIETPLTSSGLHYLQTLPLEDAVFCEPGVGLSPEYRRQSTLPAQRTLLQEHYGPFPMAEGERLRDPVTAGGKPRSFTLPRDSGLHTLLTPSSSMAEQRDPQRREERERERERERDSFLHTYIHKQHQDSLGDLYRALERASLSPLGERRLSTRLEYKHSFVRRCNDPLLNDKVHRLRILQSTLKDREGEIAVIDKLLAKPKLSSSEFQEWKRTYLELFSADAKQERQSSPVSEPSPGSTPEPTPRAPSLSHTHSYIETHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JMS3</accession>
    <name>A0AAR2JMS3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JMS3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072160.1">
        <property type="protein sequence ID" value="ENSPNAP00000051479.1"/>
        <property type="gene ID" value="ENSPNAG00000004674.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157730"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09386">
        <property type="entry name" value="LIM1_LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09387">
        <property type="entry name" value="LIM2_LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000015">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000051">
        <property type="entry name" value="LIM domain transcription factor LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF5">
        <property type="entry name" value="LIM DOMAIN TRANSCRIPTION FACTOR LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="85"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="221" mass="24147" checksum="D04DF7D944413796" modified="2024-11-27" version="1">MVNPGGSAQAAPVGAGSMSWKRCAGCGGKIADRFLLYAMDSYWHSRCLKCSCCQAQLGEIGTSCYTKSGMILCRNDYIRLFGNSGACSACGQSIPASELVMRAQGNVYHLKCFTCSTCRNRLVPGDRFHYINGSLFCEHDRPTALINGHLSSLQTNPLLPDQKVRTAPAPPARILHDFIKYTVIYQSSTADKHQMSGYTFLLYMSPALPLIILIRCLSVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JQC8</accession>
    <name>A0AAR2JQC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GNPAT</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="6">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JQC8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062062.1">
        <property type="protein sequence ID" value="ENSPNAP00000052056.1"/>
        <property type="gene ID" value="ENSPNAG00000023426.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055570"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008611">
        <property type="term" value="P:ether lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF20">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000437">
        <property type="entry name" value="GPAT_DHAPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="146"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="679" mass="76226" checksum="987D644FAE452436" modified="2024-11-27" version="1">MSAWVSSGFSGFLPQDPVLKKRDDFEDVLEERRNSSDLRYALRCYTPALYKGLVPCKASMLKSIVLQSDSLQYVVGQVSAECGESREAVQEEAALLLEEMAHRLQLGTIRFFAFTLSKAFKALFRGVCVNEEGIQRLQQAIQEHPVVLLPSHRSYMDFLMMSYILYTYDLALPVIAAGMDFISMKFVGEMLRMSGAFFIRRSFGGDKLYWAVFSEYVKTMLRNGYAPIEFFLEGTRSRTGKSLTPKTGLLNIVMEPFFKGEVFDVNLVPVSISYERILEESLYARELLGVPKPKESTSGLLKARRILSEDYGSMHVYFGHPVSIRSLAQGHISRSQHNLLPRHIPQKPTADTQAFVNDTAFRLVRLQEENMVLKPWVLLATLLLQNPDGMDLSTLTEQADWLRRLALAFGAFLDWPDHEPCSKVISSSLALHKNLVCVSGGRVRLVVEELPGAESATEEAGFNRAVSVLSCASYRNQILHLFVRPALIAVAMQTANCSRKDEVFNCFNFLRNVFSNEFILCPGAAVQDFEEACYLLGKCGAVQELQQDLVMSQSGQTTLAFLCSVLDPFLQGYQVVCRYLCEELEENLLENKFVPAVRSFTLQLILSGSLKCYEALSSDLQKNALAALIRLKAVRRVKAGDQVTLKVNKIAVNSLEDTLGKTNVQHINIYLYSSLLNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JW21</accession>
    <name>A0AAR2JW21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">sphingomyelin phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JW21"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086182.1">
        <property type="protein sequence ID" value="ENSPNAP00000056200.1"/>
        <property type="gene ID" value="ENSPNAG00000007986.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009166"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF24">
        <property type="entry name" value="PHOSPHODIESTERASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="16">
        <location>
            <begin position="31"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="447" mass="49732" checksum="98614F74D018EC0E" modified="2024-11-27" version="1">MCVVPSYLLPPPASLSLFGENCCGGIRYLSKHCAQRYTMIGDLLSKEQHDVALLQEVWSEKDFHVLKKKLGGTHPYSHYFKSGFFGSGLAVFSKHRIHDAFLYKYTLNGYPYMAHHGDWFGGKAVGKVLLNISGLAIHVFVTHLHAEYCREPDSYLPHRVVQAWELQNFIRHTSAGADVVILGGDLNMHPTDLGIRLLQSYTGLKDCYNETASFDGFEQGFTHITDNPFTHKQELIPFGGGIRIDYVFFKGSKSVDVSCESLSTTKGPVPGQPFPYSDHEALSTELELHPSEDGKGYSNQEKDCTTDKLSELVNIVTEARTEVKVGLHCAERMRYTAARTGIMGLALLLLELAIAAVPCFALGTEQPFPKASFYLLGALCFAILFCTTLLYIFYGMEVKALQGAEDQMRLALGSLQERLKAFPKGKYEGRSQTELDSRDPSTLEPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JZT3</accession>
    <name>A0AAR2JZT3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JZT3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085726.1">
        <property type="protein sequence ID" value="ENSPNAP00000057563.1"/>
        <property type="gene ID" value="ENSPNAG00000033022.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108438435"/>
    <dbReference type="GeneTree" id="ENSGT01030000234640"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF860">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="312" mass="36009" checksum="5E291DC4C54CF70A" modified="2024-11-27" version="1">MDYSTNITYLFLEGHVELEKYRYFYFVITFTVYTLILCCNAVVISVIYTNKPLHEPMYIFIAALLCNSLFGTAAFYPKVLSDFLSEKQIISHGACLFQAFCLYTYAAAEFALLSAMAYDRYVSICKPLQYATLVKMSTVKKILFFCWFVPSCEIGISIILTYRQLCKFKLNRVYCSNYTIAKLSCGDTTVMNTYGLFALAIAVVPPMTFIFYSYVRIIYVCLKNSKDFRRKALQTCFPHLCILTTFSCTACFEVINSRFEGNVPHIFAMIMSVENMVISPLLNPIVYGLKLQEISKRIKRLIWKRKMHIFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K116</accession>
    <name>A0AAR2K116_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import receptor subunit TOM70</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial precursor proteins import receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Translocase of outer membrane 70 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Translocase of outer mitochondrial membrane protein 70</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553538.1">
        <property type="nucleotide sequence ID" value="XM_017698049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K116"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068491.1">
        <property type="protein sequence ID" value="ENSPNAP00000057940.1"/>
        <property type="gene ID" value="ENSPNAG00000027449.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427689"/>
    <dbReference type="CTD" id="28185"/>
    <dbReference type="GeneTree" id="ENSGT00940000157095"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000141">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000071">
        <property type="entry name" value="Putative mitochondrial import receptor subunit TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 19" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="334"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="368"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="443"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="512"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="575" mass="64753" checksum="2640EAFB789755F9" modified="2024-11-27" version="1">MAASKPVEPQSGGGLPRWQLALLLGAPVALGLGAAYLWQRSRAKEKRAGERKTPEGSASPVQGQRAATQPEHEDMSPLDRAQGAKNKGNKYFKAGKYEQAIQCYTEAISLCPKEQKSDLSTFYQNRAAAYEQQAKWTEVVQDCSQAVEMNPRYVKALFRRAKALEKLDKKKECLEDVTAVCILEAFQNQQSMLLADKVLKQLGKEKAKEKYKNREPLMPSPQFIKSYFSSFTDDIISQPLQKGEKKDEDKDKEGEAAEVTESSGYLKAKQYMEEENYDKIISECTKEIESKGRHTAEALLLRATFYLLIGNATAAQPDLDKVINMQDANVKLRANALIKRGSMYMQQQQPQLSTQDFNMAAEIDPRNADVYHHRGQLKILLDQVEEAVADFDECILLRPDSALAQAQKCFALYRQAYTGNNPSQVQTAMDGFEDVIRRFPKCAEGYALYAQALTDQQQFGKADEMYDKCIELEPDNATTYVHKGLLQLQWKQDLDMGLDLISKAIEIDNKCDFAYETMGTIEVQRGNLDKAIDMFNKAINLAKSEMEMAHLYSLCDAAYAQTEVARKYGLKPPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K214</accession>
    <name>A0AAR2K214_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein kinase Sgk1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Serine/threonine-protein kinase Sgk3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Serum/glucocorticoid-regulated kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Serum/glucocorticoid-regulated kinase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">SGK3</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Protein kinase that may play an important role in cellular stress response. May be involved in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2K214"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067938.1">
        <property type="protein sequence ID" value="ENSPNAP00000056474.1"/>
        <property type="gene ID" value="ENSPNAG00000003699.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153776"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06870">
        <property type="entry name" value="PX_CISK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05604">
        <property type="entry name" value="STKc_SGK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000065">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000018">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000030">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037900">
        <property type="entry name" value="CISK_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037709">
        <property type="entry name" value="SGK3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 22 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 22 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 23" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PX" evidence="25">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="154"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="26">
        <location>
            <begin position="412"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00074735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00077830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="488" mass="56685" checksum="9621E999CCD5735F" modified="2024-11-27" version="1">MDEHSSCPNVSIPCYNEQRDKKKRYTVYKVMVSVGRHEWFVFRRYTEFDKLYNTLKKQYPALNLKIPAKRIFGDNFDPEFIKQRRTGLHEFIQKIVSHPQLSNHPDVRDFLQMDKSKNFSDASEDEDEKVCGVSHLLWLLFGSLFLSRAKPTDFDFLKVIGKGSFGKVLLAKRKRDGKCYAIKVLQKKVILNRREQKHIMAERNVLLKNVKHPFLVGLHYSFQTTDKLYFVLDFVNGGELFFHLQKERTFPEPRAKFYIAEMASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKEGISQADTTTTFCGTPEYLAPEVLRKQPYDNTVDWWCLGSVLYEMLYGLPPFYSRDTHEMYENILHKELPMRPGASTTAWSILQALLEKDNTRRLGYRDDFNEVKSHDFFSSINWDDLEQKKIPPPFNPSVESQYDISNFDPEFTEETVPNSVCISTEHSIVNASVMEADDAFLGFSYAPPSDDSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K2T7</accession>
    <name>A0AAR2K2T7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 24 25">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="14 24 25">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CDS2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>1,2-di-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77126"/>
            <dbReference type="ChEBI" id="CHEBI:85351"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:45657"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45672"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77128"/>
            <dbReference type="ChEBI" id="CHEBI:85355"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:45673"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45676"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:85356"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:45677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45652"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:72864"/>
            <dbReference type="ChEBI" id="CHEBI:85350"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:45653"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45668"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77130"/>
            <dbReference type="ChEBI" id="CHEBI:85354"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:45669"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45648"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:85349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:45649"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45660"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77098"/>
            <dbReference type="ChEBI" id="CHEBI:85352"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:45661"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45664"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74560"/>
            <dbReference type="ChEBI" id="CHEBI:85353"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:45665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16230"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11 24 25">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 24 25">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="14 24 25"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2K2T7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048825.1">
        <property type="protein sequence ID" value="ENSPNAP00000056807.1"/>
        <property type="gene ID" value="ENSPNAG00000007488.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158877"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF4">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018269">
        <property type="entry name" value="PC_trans_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="20 24" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="18 24 25" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="22 24" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="23 24" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16 24 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="17 24 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="230"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00000859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00001056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00001617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00001729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00001902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRNR" id="PIRNR018269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="454" mass="51996" checksum="5EFCAECDC1F36796" modified="2024-11-27" version="1">YMFSCLLTQIANQPITRLQLNAFRPGSEAEDKIEKDGGASDSECKADTGVPEVPPPADDTPEVLNKALSGLSSRWKNWWVRGILTLAMIAFFFLIIYLGPMVLMMIVLCVQIKCFHEIITIGYSVYHSYHLPWFRTLSWYFLLCVNYFFYGETVTDYFFTLVQREEPLRILSKYHRFISFALYLSGFCMFVLSLVKKHYRLQFYMFGWTHVTLLIVVTQSHLIIHNLFEGMIWFIVPISCVICNDIMAYMFGFFFGRTPLIKLSPKKTWEGFIGGFFSTVVFGILFSYLMAGYRYFVCPVEFSNDSNSFTVDCEPSELFQLQDYTLPSVLESITGWTTVKLYPFQIHSIALSAFASIVGPFGGFFASGFKRAFKIKDFANTIPGHGGIMDRFDCQYLMATFVNVYIASFIRGPNPTKVIQQLLALRPDQQLHIYNSLKAHLTERGLLAALEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KAR7</accession>
    <name>A0AAR2KAR7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KAR7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068282.1">
        <property type="protein sequence ID" value="ENSPNAP00000059191.1"/>
        <property type="gene ID" value="ENSPNAG00000033212.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163793"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="119"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="261" mass="30230" checksum="DD9B60C1C37942E4" modified="2024-11-27" version="1">MDRSLFCSLWDYMRLHHSDTLRSPLFPVILSVSSYFVLCLPYLLCDLVGEKWPAVHRYKIQPCKLPTASMIRRCIGITLRNHIFLVFPAAVAQWAWRPPVPLPEKAPMLLELVGGVVGNLLLFDLQYFIWHLLHHKVRWLYVTFHAIHHEFSAPFVLATQCIGGWELVTVGFWTTLNPILLRCHILTAWAFMVAHVYVSVEDHCGYDFPWSSSRLIPFAIYGGPSKHDVHHQKPNTNFAPHFSHWDKMFGTHADFSFVKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KF30</accession>
    <name>A0AAR2KF30_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KF30"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048576.1">
        <property type="protein sequence ID" value="ENSPNAP00000061079.1"/>
        <property type="gene ID" value="ENSPNAG00000036783.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182912"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF25">
        <property type="entry name" value="ZINC FINGER (C2H2)-60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="178" mass="20465" checksum="CCF8A4BE18B60B35" modified="2024-11-27" version="1">MARHDRLKKKKKKKFMFMLDHYTPKLLAIFQEKKGAVGERHRTEMNILLQSGTTEKTREVVIRCLIDHLGEDNFEDTNHRAINVEEELAAETMAVFLVRDESGQVENVGIVIEGSIVLDHLGDLSRGCCYLLGLTYALDLKYPKTLKYIFEVFQKILLEVDPGKLSTKVLRLKNNLMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KPY3</accession>
    <name>A0AAR2KPY3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">RAR-related orphan receptor B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RORB</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE). Required for proper cerebellum development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KPY3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046623.1">
        <property type="protein sequence ID" value="ENSPNAP00000064186.1"/>
        <property type="gene ID" value="ENSPNAG00000009139.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157708"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06968">
        <property type="entry name" value="NR_DBD_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06939">
        <property type="entry name" value="NR_LBD_ROR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000005">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000003">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044101">
        <property type="entry name" value="NR_DBD_ROR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003079">
        <property type="entry name" value="ROR_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805:SF6">
        <property type="entry name" value="NUCLEAR RECEPTOR ROR-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01293">
        <property type="entry name" value="RORNUCRECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="14">
        <location>
            <begin position="17"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="15">
        <location>
            <begin position="228"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="57973" checksum="17C024352EB5BE91" modified="2024-11-27" version="1">LIYSVFDHFTQVAQIEVIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQNNASYSCPRQRNCLIDRTNRNRCQHCRLQKCLALGMSRDAVKFGRMSKKQRDSLYAEVQKHQQRLQEQRQQQQTGEAEALARVYSSGLSSGLGSLGHDIGGAYSNGHLIDMPKGQANGAPAGYYGMDSTQPSPDQSGLDMAGMKQIKQEPIYDLTPVPNLFPYSSYQDSQLAPGVSMGELDRIAQNIIKSHLETCQYTAEELQQLAWQTHSYEEVKMYQSKSREVLWQQCAIQITHAIQYVVEFAKRITGFMELCQNDQILLLKSGCLEVVLVRMCRAFNPLNNTVLFEGKYGGMQMFKALGCDDLVSAVFDFAKSLCSLQLTEEEIALFSAAVLISTDRPWLMEPRKVQKLQEKIYFALQHIMQKNHMDEDALAKLIGRIPTLAALCTLHTEELQAFQQLHPETVNVLFPPLYKELFNPDAQFKLSFNWVSSSGSLTFSFFIRHCNIEKGMRVGFEDDNIAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KT10</accession>
    <name>A0AAR2KT10_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KT10"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059709.1">
        <property type="protein sequence ID" value="ENSPNAP00000065266.1"/>
        <property type="gene ID" value="ENSPNAG00000031694.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182961"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15278">
        <property type="entry name" value="7tmC_RAIG2_GPRC5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051753">
        <property type="entry name" value="RA-inducible_GPCR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511">
        <property type="entry name" value="G PROTEIN COUPLED RECEPTOR, CLASS C, GROUP 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511:SF9">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY C GROUP 5 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="8">
        <location>
            <begin position="110"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="410" mass="44545" checksum="58F05480C927FA9D" modified="2024-11-27" version="1">MEVWVARAIMCLFFHFLCQVAYFPVLIFLLPIMAMSFAHKEEPVVPWGCGFGLTRPYTLLCDLETIWGVVVEGLAAGGTVAAILLALVLLCRLRSVTEAEKRSGVGPILLLLVGIVGLFGLSFAYLIEHNESLCVIRRALWGALFALCFSCMLAQGVRLRQLVREKRCPGGCALTGLALGLAAVQGIVAAEWLLLTVLRQGQPACQYEPLDFALACSYVLALLLAALVAAVLALCGRAQPWRCNAVWLLVSCLLSILLWAAWMGFYLYGNAALGKAPEWDEPALAVALVAQGWLLLLCHAAPEAHGCLQPPPQPSAPDYFDTSQNSTRLRETSFDEDIPLSHRQFVENQGYAFDENAAGLRSGGHHNGNTGTRPSAPFRSNVYQPTEMTMILNGGTVPSAPPTYTGRQLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KX24</accession>
    <name>A0AAR2KX24_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017572161.1">
        <property type="nucleotide sequence ID" value="XM_017716672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KX24"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060380.1">
        <property type="protein sequence ID" value="ENSPNAP00000066696.1"/>
        <property type="gene ID" value="ENSPNAG00000036505.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108438690"/>
    <dbReference type="CTD" id="100124615"/>
    <dbReference type="GeneTree" id="ENSGT00950000182969"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF367">
        <property type="entry name" value="KERATIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="4">
        <location>
            <begin position="58"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="297"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="382" mass="44384" checksum="2A19C8EE285C7B94" modified="2024-11-27" version="1">MSFSSRSYGTYGYRALSMYGGAGGQGARISTSSRGSLYGSVWGLNLADGLDLHISTNEKVTMQNLNNRLASYMQEVHSLEKKNAKLEKNIAEWYESHNIIFCDHTDFLEKVKDLREQIEYITKCNAKTVLDIDNFRLASKDFQMMYETERNMHVVLEEDTTCIRKVLDEMTFFRSDLELQYESLKEEFIMLKMNHEQEMAEVREQATQVNVSVDAAPSTDLNKALSEIRQHYEALTAKNRSDLEMWFQSKISTVEQDVVTHSEELHYSKTELKELKSTFQTLQVELQTHHSMRMSNEESLAETEAQYSEQLAELQRTINNLELQLYHIHSDMTSNKREYDILLGLKNHLEREITEYRLLLEGEKSEHQIAHKTTTVVETLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L0X4</accession>
    <name>A0AAR2L0X4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SPRY domain-containing SOCS box protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPSB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565051.1">
        <property type="nucleotide sequence ID" value="XM_017709562.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L0X4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068358.1">
        <property type="protein sequence ID" value="ENSPNAP00000070278.1"/>
        <property type="gene ID" value="ENSPNAG00000031487.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108434445"/>
    <dbReference type="GeneTree" id="ENSGT01030000234629"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12876">
        <property type="entry name" value="SPRY_SOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000079">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035754">
        <property type="entry name" value="SPRY_SPSB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF16">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="6">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="7">
        <location>
            <begin position="176"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="227" mass="25587" checksum="5D9B61CF8912824B" modified="2024-11-27" version="1">MSVLRDHTCESWEWDTEGKSPDAYLSPCRQAVYFHISPLLESQGTAGVRGTKGFSYGEHYWEIEFLEPPYGSSVMVGVGTVNARLHAGEQFINLIGMDAESWGLSYKGFLWHNGQSRKYTEPFYDQNTVIGVLLDRTTGTLTFYRNGENLGLAFSNLDQVSNALFPMVSSTAPETELQLGLRTFRLSSLQEHCIHTITHCLGRLGHTHLLPLPRVLRCQIHTYSIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L3M5</accession>
    <name>A0AAR2L3M5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">EIF3A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L3M5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065570.1">
        <property type="protein sequence ID" value="ENSPNAP00000068971.1"/>
        <property type="gene ID" value="ENSPNAG00000034747.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271858"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF170">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="334"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="597"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="782"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="702"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="782"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="958" mass="113791" checksum="7EA9DA84B186ED23" modified="2024-11-27" version="1">MMEKIVEENRRSLNSEEFEETSLALEQKQSEQKTEPQYKETTTVEVHDQTEGQEHEETEELQEYLQTEMEEHEEMTELCEYDQTKQQEFEIELQKKKKKKKRLSNILSASKQRLNLVLLGLDKEAKTSISEILLGKKFSSSHQRVKPKSSPVCVRKKGKVCGCLVTLVEMPALYNTQLSEEKVMQETLHCVSVCDPGVHAFLLLIPEGCLTDEDKGVIEKIQKVFGSGINNHTIVLISQQSQNKELNASVTKVIKEFGVQYKFVDSKTDATQLVTFFKSFHSKSKDGCYTMASYANAQVETQLQYKRQIQNLQQKINKLTLTNWSQTEGSSKGPEALRIVLLGKTGVGKSATGNTILGKEDVFTEDIGGSVTVVCQKESADINGRQITVVDTPGLFDTNVPNVEITKEITKCISMAAPGPHVFLLVLSIGQRFTQEEQDTVNMIKDTFGEKYKMYTIVVFSKGDLLKENTIEQYIEKPGPTMKRFLFDFGNRYHVLNNSNKSSSTQVTDLLEKINSMVTVNGGSCYTNDMFQQVEKALQEEQERILKEREGEIEREKERLKAKYEAEMERMRIEVQKEKEKQEAEGRRREKEFKEKELDIKREMTERERIQREDVTKRREEDEKKMQRWMAEIHREKEENSQRWEKQREEDQRRRDQEEEERRKKEEEWRKKQKEERENFEKESEDMEKKVREELMNLQQDYKQKAEEEEKRRKELEEKIQHAEESKKKELQELQLTQQREWIKRMEEEEKRREKQQKYWEKTIASMEEAWTLQQIRKQKQYEWEKQKEKEERDLKEKERKEKEEQERKRIENEANEKIRQMKEQLEAEREKDEKERNEKEEQLRKEMEEQLQKQLKSFRREKEEEEIIRNEAEKRNVNFIVETHTREMENLKRQTEQTARKQAEEEFHAKLDEKVKEARDKGFVEGCAEIEAARTALGRRVDRFVHHVCHTKKKKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L5P7</accession>
    <name>A0AAR2L5P7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial 2-oxoglutarate/malate carrier protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Solute carrier family 25 member 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SLC25A11</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the transport of 2-oxoglutarate (alpha-oxoglutarate) across the inner mitochondrial membrane in an electroneutral exchange for malate. Can also exchange 2-oxoglutarate for other dicarboxylic acids such as malonate, succinate, maleate and oxaloacetate, although with lower affinity. Contributes to several metabolic processes, including the malate-aspartate shuttle, the oxoglutarate/isocitrate shuttle, in gluconeogenesis from lactate, and in nitrogen metabolism. Maintains mitochondrial fusion and fission events, and the organization and morphology of cristae. Involved in the regulation of apoptosis. Helps protect from cytotoxic-induced apoptosis by modulating glutathione levels in mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(S)-malate(in) + 2-oxoglutarate(out) = (S)-malate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71587"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>maleate(in) + 2-oxoglutarate(out) = maleate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71599"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30780"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>malonate(in) + 2-oxoglutarate(out) = malonate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71591"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>oxaloacetate(in) + 2-oxoglutarate(out) = oxaloacetate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71603"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>succinate(in) + 2-oxoglutarate(out) = succinate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71595"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with SMIM26.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037389621.1">
        <property type="nucleotide sequence ID" value="XM_037533724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L5P7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068977.1">
        <property type="protein sequence ID" value="ENSPNAP00000069967.1"/>
        <property type="gene ID" value="ENSPNAG00000037178.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430393"/>
    <dbReference type="GeneTree" id="ENSGT00940000158465"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015139">
        <property type="term" value="F:alpha-ketoglutarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000013">
        <property type="entry name" value="Mitochondrial 2-oxoglutarate/malate carrier protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 19 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="19">
        <location>
            <begin position="17"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="19">
        <location>
            <begin position="111"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="19">
        <location>
            <begin position="211"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="308" mass="33992" checksum="51C4227892DAA4E1" modified="2024-11-27" version="1">MAAPANTVKPKTSPKSIKFLFGGLAGMGATVFVQPLDLVKNRMQLSGQGSRAREYKTSLHAVVSILRNEGIRGIYTGLSAGLLRQATYTTTRLGIYTVLFEKMTKADGTPPNFFMKAMIGMTAGATGAFVGTPAEVALIRMTSDGRLPADQRRGYTNVFNALFRITKEEGVTTLWRGCIPTMARAVVVNAAQLASYSQSKQALMETGYFSDNIFLHFCASMISGLVTTAASMPVDIAKTRIQNMRMIDGKPEYKNGLDVLLKVVRNEGFFSLWKGFTPYYARLGPHTVLTFIFLEQMNKFYRIYFLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L6Z8</accession>
    <name>A0AAR2L6Z8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L6Z8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069332.1">
        <property type="protein sequence ID" value="ENSPNAP00000072373.1"/>
        <property type="gene ID" value="ENSPNAG00000036723.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164961"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="261"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="434" mass="48808" checksum="A4AEEC093AD24411" modified="2024-11-27" version="1">MPVRPGKPKRIVRVCWERLAEEPVRLIFNSHIRQNFDQISGEVGDIDSEWAMFRSSIAEAADCSCGRKVVGACRGGNPRTRWWTPQVRDAVKLKKESYRAWLACGTPEAAGRYRQAKRSVASVVAKAKTRVWEEFGEALETDFKSAPKRFWQTVRRIRRGKQCATSTVYSGDGVLLTSTEDVIGRWKEYFEDLLNPTDTFSSEEAESGDMGIGLSITEAEVAKVVKKLLGGRAPGVDEIRPEFLKALDVVGLSWLTRLFNIAWTSGAVPLDWQTGVVVPLFKKGDRRVCSNYRGITLLSLPGKVYAGVLEKRVRLIVEPRIQEEQCGFRPGRGTLDQLFTLSRILEGSWEFAQPVHMCFVDLEKAFDCVPQGILWEVLREYGVHGSLLRAIQALYKQSRSLVRIAGSKSDSFPVRVGLRQGCPMSPILFIIFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L7J1</accession>
    <name>A0AAR2L7J1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Neuromedin-K receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">NK-3 receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Neurokinin B receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Tachykinin receptor 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L7J1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059845.1">
        <property type="protein sequence ID" value="ENSPNAP00000072510.1"/>
        <property type="gene ID" value="ENSPNAG00000003226.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155512"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004995">
        <property type="term" value="F:tachykinin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902093">
        <property type="term" value="P:positive regulation of flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000291">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001681">
        <property type="entry name" value="Neurokn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001013">
        <property type="entry name" value="NK3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR TKR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925:SF1">
        <property type="entry name" value="NEUROMEDIN-K RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01026">
        <property type="entry name" value="NEUROKININ3R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00244">
        <property type="entry name" value="NEUROKININR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 18" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 18" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 18 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="21">
        <location>
            <begin position="67"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="123"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601681-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="422" mass="47632" checksum="CA5637B2DAB471A2" modified="2024-11-27" version="1">TTCCRGPAALSVELVLFVCLFVCTMSLSNSSNSTNSSQFAHRFVQAPWRVALWAMAYVAVMLVALTGNALTAWVILAHRRMRTVTNYFLLNLAVADASMAALNTPVNFIYAARGEWYFGLGYCRFHNFYPVAALFASIYTMTAIAVDRYMAIIHPLKPRFSATVTKVVIVCVWGAAVVLAFPLCFYSNTRNVPHRTLCYVAWPRRADDAFMYHIIVAVLMYLLPLVVMAITYSKVGVTLWGSSVPGYSSENYLEQLQAKRKVVKMMVVVVVTFAVCWLPYHVYFIVTGLNRQLSKWKSIQQVYLAVVWLAMSSTMYNPIIYCCLNSRFRAGFKRAFRWCPFIHMSQSDEMELRITRFHPTRQSSMFTLTRLESSTDPLTQSSRRKSSSTSRHSSLCGQTRPNAARLSLNSFHNGTAVHSAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LG96</accession>
    <name>A0AAR2LG96_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">RFFL</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017552242.1">
        <property type="nucleotide sequence ID" value="XM_017696753.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552243.1">
        <property type="nucleotide sequence ID" value="XM_017696754.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552244.1">
        <property type="nucleotide sequence ID" value="XM_017696755.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552245.1">
        <property type="nucleotide sequence ID" value="XM_017696756.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LG96"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064956.1">
        <property type="protein sequence ID" value="ENSPNAP00000075628.1"/>
        <property type="gene ID" value="ENSPNAG00000032536.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426933"/>
    <dbReference type="CTD" id="117584"/>
    <dbReference type="GeneTree" id="ENSGT00390000012719"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000110">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049320">
        <property type="entry name" value="CARP1_2_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055111">
        <property type="entry name" value="RNF34_RFFL_HeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057299">
        <property type="entry name" value="RNF34_RFFL_SAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RIFIFYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21272">
        <property type="entry name" value="FYVE_CARP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22968">
        <property type="entry name" value="RNF34L-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23632">
        <property type="entry name" value="SAP_RNF34_RFFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="21">
        <location>
            <begin position="49"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="309"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="139"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="356" mass="39933" checksum="AE465DB44A75E432" modified="2024-11-27" version="1">MWASCCNWFCLDSSNMEESGTIRTNSRHQAYTNSGYSSYPSPPPPDHTCKACGRRFDTLTRKHICVDCKKSFCGRCSAQSVPLPRLCLTCHRFHGTMFERAELMKLKVKDLRDYLHLHEVPTQMCREKEELVDLVLGQHTPTSDTSTPTQTHDHTQPSEQEAPSVPTPATVQPRAGNSVTTDVASQSHTAVDEQSPSVAEEPVHIEVDTELQGEQESQSSDSEEVLPSGRRASLSDLGSVEDIEALSVRQLKEILARNFVNYKGCCEKWELMERVTRLYHDQKDLQNLVSNTSDGTDTTGRPSMEENLCKICMDSPIDCVLLECGHMVTCTKCGKLMNECPICRQYVVRAVHVFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJB7</accession>
    <name>A0AAR2LJB7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">IMD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MTSS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJB7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083241.1">
        <property type="protein sequence ID" value="ENSPNAP00000074767.1"/>
        <property type="gene ID" value="ENSPNAG00000016828.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183156"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07643">
        <property type="entry name" value="I-BAR_IMD_MIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22060">
        <property type="entry name" value="WH2_MTSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000010">
        <property type="entry name" value="Metastasis suppressor 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF8">
        <property type="entry name" value="PROTEIN MTSS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="9">
        <location>
            <begin position="47"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="8">
        <location>
            <begin position="746"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="298"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="492"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="573"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="721"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="298"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="503"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="588"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="725"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="774" mass="84389" checksum="3EC3D147C5148EBC" modified="2024-11-27" version="1">QGFVPTAAISWWYGQHKVCNNVCLSIFHCFIMVCYFPIQRLQCYQHIHEKLLHTLSVLGLTGVFVLLQCSYPVWEDFSAKATKLHAQLRTTVLAAVAFLDAFQKVADMATNTRGATRDIGSALTRMCMRHRSIEAKLRHFTNALMESLINPLQERIEDWKKTANQLDKDHAKEYKRSRHEIKKKSSDTMKLQKKARKGRGDLQPQLDSAMQDVNDMYLLMEETEKQAVRRALVEERGRFCTFISFLQPVVNGEIAMLGEITHLQAIIDDLTVLTTDPHKLPPASEQVIKDLKGSDYSWSYQTPPSSPSSSGSRKSSMCSAHSSASRSSGGSQTHSPSSSCRYRSLAQPLPGGAHRLSSVSSHDSGFISQDANVYSKPPSPMPSDITSQKSSSSASSEASETCQSVSECSSPTTDWSKAGPHEQPLVNTLQRRKEPLDRLRESEASPQSQGYAGVHPEDPQRPRMTPATIAAKHGEEVSPAASDLAMVLTRGLSMEQQKSSRDSLQYSSGYSTQTTTPSCSEDTIPSQGSDYDCYSVNGDAEADGQTDFDKSSTIPRHSNIAQNYRRMIQTKRPASTAGLPSGAGAQGGTATIRRTPSTKPGVRRTLSNAGPIPIRPPIVPVKTPTVPDSPGGAYPGPPARVGSEECVFYVADDASPSALEYVKASPKRLSLPNTAWGAGAVEMSVYGQPGEEDQLLAANRHSLVEKIGELVASAHALGEGQFPFPSLPDRPPPGLEAQADPQEPQDGEDMLVSIRRGVRLRKTVSNDRSAPRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLW4</accession>
    <name>A0AAR2LLW4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dual-specificity kinase</fullName>
            <ecNumber evidence="4">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TESK1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLW4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074829.1">
        <property type="protein sequence ID" value="ENSPNAP00000075662.1"/>
        <property type="gene ID" value="ENSPNAG00000014338.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157807"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14155">
        <property type="entry name" value="PKc_TESK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000202">
        <property type="entry name" value="Dual specificity testis-specific protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000134">
        <property type="entry name" value="Dual specificity testis-specific protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF3">
        <property type="entry name" value="DUAL SPECIFICITY TESTIS-SPECIFIC PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="44"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="348"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="555"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="702" mass="77191" checksum="6E61DEFCDBF48473" modified="2024-11-27" version="1">MDPEEPGQEVWEPPPHGLHGPNRVRPSSYRALRSAVSSLARIDDFFCEKIGSGFFSEVFKVQHRITGQVMALKMNTLASNKANMLREVQLMNRLCHPNILRFLGVCVHEGQLHALTEYINGGNLEQLLDSDMYLSWVVRIGLSLDIARGLQYLHSKGIFHRDLTSKNCLVRCENGLFTAVVGDFGLAEKIPDYSDGVEKQPLAIVGSPYWMAPEVLRGEFYNEKVDVFAYGIILCEIIARIEADPDVLPRTEDFGLDVQAFEHMIGDCPPAFLSLAVNCCNMCAKSRPSFSDIVVSLERMEREREKTETPVILGEWCCKQKYMKERILFIFCIFFGDQKFLSTRLMTQTDDKSPYRRRNSPCPVGDQRLSRSQSDMLPPAASPCLGTPARVNPFSLRHDLNGGRIKLFDTPSKSVISLTFTLPPPPDPCASPPLGGISPPRRCQSLPCTPELGRPLALFREPETLSVEEEQEEKGQELKNGGMEERGKGLEVSDNLCQDSGLALELEMVSLERVEEEDEGQAEAEGLYCSEPMDCSSSPEGALSMTGKTLFISSSSSSSSSSCRQPNGWPRPVSNGPPSLPPLPRLDNNNSCLLGAGRIPSWARLNSYRGRRAQEPLAPPAEQDEVISCPSCCLVGFSFPSVCLRGAPPTRPLPRPYRNLNGREAAAATATATKGLLCRGANGLAVATAPCEPGLPLPEAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LM76</accession>
    <name>A0AAR2LM76_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LM76"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060726.1">
        <property type="protein sequence ID" value="ENSPNAP00000077660.1"/>
        <property type="gene ID" value="ENSPNAG00000035220.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162700"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="70"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="247"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="519"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="560" mass="61520" checksum="DC4A5CC9CDF57B1A" modified="2024-11-27" version="1">MQGGGDTEWRYSWNKDDYSHNSFSTRKENTISPVRESDSGKYTCRGQRRSDSQTSEISDAVTLTVSDRAQAVLRSSPQSWLTEGDSVTLSCEVRGSSTGWTFSWYRDKDELLSDSSRGAGGSYTLSPAALNHTGVYACRAMRGETAYHTQYSDTQPLWITGVSPPVSLMVSPSRAEHFITDSLSLSCEGQSDSTGWRVRRYTHSEKVSDCSSGWGSAAGTTCSISSLITSHTGVYWCESESGESSNPVNISVTFGFIILDSPVHPVTEGDPLTLRCLYHHTKPSNLTAEFYKNGSLLQTQTTGEMTIHTVSKSDEGLYHCKHPEKGESPQSWISVRVSTRSAAKSAIGLVGVVVGVSFAVLFIILLILLWCYRTRKDGQSLSHFTGHQQNINQTSVQNHRESGAGDSQSGYMQAEHVYDTVERAGRINKSNYFLSNIIDSYSFAVTNTLYSVSGDAADELSDVTYSQIMRKNRKSKNKGKYIPAKPSQTETIYSEVQPGKTTDADTGSMAVTYAEIELKTQKKPKKKTEQTSIDADTVYSELKQNTDKGDVTGLDDSTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LM85</accession>
    <name>A0AAR2LM85_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Caspase 7, apoptosis-related cysteine peptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CASP7</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LM85"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064902.1">
        <property type="protein sequence ID" value="ENSPNAP00000077670.1"/>
        <property type="gene ID" value="ENSPNAG00000034914.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426491"/>
    <dbReference type="CTD" id="840"/>
    <dbReference type="GeneTree" id="ENSGT00940000153232"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097194">
        <property type="term" value="P:execution phase of apoptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1470:FF:000002">
        <property type="entry name" value="Caspase-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000001">
        <property type="entry name" value="Caspase-3 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454">
        <property type="entry name" value="CASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454:SF31">
        <property type="entry name" value="CASPASE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="15">
        <location>
            <begin position="142"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="14">
        <location>
            <begin position="288"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="381" mass="43465" checksum="BA2213117056605E" modified="2024-11-27" version="1">MGGCSKVATVLDDWRLTLRRLARKFYSSFVSTFTFFLLIQRWVNISVSRINVGSRQLTTSRSALAENSNFRMEEETLPAETEMYEDGGEGDTTDAKPDRKARFLLFGSKKNKDGKLREKDHSSEGHYRIVSPAFQYKMSHRRVGKCIIINNKNFDEKTGMNVRNGTDRDAGELFKCFKNLGFDVFIYNDQTCAKMERLLKEVSEEDHSDSSCFACILLSHGEEGMIYGTDGAMPIKSMTSLFRGDMCKSLVGKPKLFFIQACRGSEFDDGIQTDSGPPNDTIETDANPRHKIPVEADFLFAYSTVPGYYSWRNPGRGSWFVQALCNVLNEYGKQLEIMQILTRVNYMVATCFESWSEDPRFSEKKQIPCVVSMLTKELYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZ63</accession>
    <name>A0AAR2LZ63_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Epidermal growth factor receptor kinase substrate 8-like protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Epidermal growth factor receptor pathway substrate 8-related protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EPS8L2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton. In the cochlea, is required for stereocilia maintenance in adult hair cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EPS8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LZ63"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079003.1">
        <property type="protein sequence ID" value="ENSPNAP00000080024.1"/>
        <property type="gene ID" value="ENSPNAG00000029786.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160990"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900029">
        <property type="term" value="P:positive regulation of ruffle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01210">
        <property type="entry name" value="PTB_EPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09540">
        <property type="entry name" value="SAM_EPS8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11764">
        <property type="entry name" value="SH3_Eps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000023">
        <property type="entry name" value="Epidermal growth factor receptor kinase substrate 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000218">
        <property type="entry name" value="Epidermal growth factor receptor kinase substrate 8-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000180">
        <property type="entry name" value="epidermal growth factor receptor kinase substrate 8-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039801">
        <property type="entry name" value="EPS8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055093">
        <property type="entry name" value="EPS8_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033928">
        <property type="entry name" value="EPS8_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035462">
        <property type="entry name" value="Eps8_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287:SF20">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE 8-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE EPS8-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22975">
        <property type="entry name" value="EPS8_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="486"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="436"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="442"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="700" mass="79550" checksum="F5835DF1E2794A20" modified="2024-11-27" version="1">MVFNCLQCGHERQRCSLTSCFEIWLKSCVYSVGIVNASQTIFFSILEQRKKYSNSNFIMQETSQYHVQHLSTFIMDKTESIATIDDAVKKLILLDSKDKIWTQEMLLQVSDKAIKLLDCDTMEELENFPLSTVHHSQTVLNKTRYPSVLLLVCQDNEQHKPDIHFFCCDEVEAEIVHADIDSALGDSKHGKKMRPQTLKMNQEKMKRQRETIIPPSESKSSNPGAKGRVAAMDMKNAERRDSAQQDPESHEKLAQRIEKDVQILNCALDDIEIFVARLQKAAEAFAQLNQRNKSRKNKKRGPAEGMLTLRAKPPSEAEFIDSLQKLKLSFNLLAKLKKHIQNPSASELVHFLFGPLEMSCGTPELPRSVISPHLSRDAVDFLRGHLSPKEMTIFELLGDGWTRPRADWPRDQCAPLYVPRFRNGWEPPVEPFRTSPWETECSTSMSPSSSDSSAHSPHCHQTGESKPECASLTVDFPCRSYTAPSPSRKYAKIRYHFVARNANELSVLQDEILEVIEDDKQWWKLRNRSGQAGYVPYNVLDVVTPEELHGMDPMYNQVTPTWEVIVARTHQMDEVNDELLKRITANKKQQPARNFKVERHSSRAIPLTLKSTSAEVAAWLNAKGFSKPTVDCLGVLSGAQLFSLEKEELKAVCGDEGSRVYSQLTVQKSLLEKSQGDSELQEIMRKQKEKIASATNLEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M064</accession>
    <name>A0AAR2M064_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M064"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059113.1">
        <property type="protein sequence ID" value="ENSPNAP00000079976.1"/>
        <property type="gene ID" value="ENSPNAG00000033220.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163467"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032718">
        <property type="entry name" value="PGBD4_Znf_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599:SF3">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13842">
        <property type="entry name" value="zf-Tnp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="3">
        <location>
            <begin position="124"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="PiggyBac transposable element-derived protein 4 C-terminal zinc-finger" evidence="2">
        <location>
            <begin position="539"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="597" mass="69236" checksum="AD1D47EDB1673265" modified="2024-11-27" version="1">MSTPRKRRATTEKLIKEGFSSSYEDSGSGFESGFSDDEEMFFSGMDPVQDDPRLAQHEESETPTEAHSPEAVASESESEEEKQSSQESERWKNVDEDDAEPFPFQFRPNRTPGYLLEHTQEYGPLDLFLLFFSMDVIASLCINTNKNAKVKKQKGKKYKWEPIEIGEFMKFCGMLVFMGVIRCPSIRDYWRTDNVWQTPFVRRIMRRDRFQAISWSLHISDPDKDSENNANKGTAGYDRLMKIRSLMESLKVACKAFYHPKKHLSIDERMVPTKAKNGLKMYIKSKPHKWGYKLFVLCDSANGYTCDFIVCTGKSQFKSPHGLSYDSVVSLLNPSFLGTGYVLYCDSFYTSPTLFRDLYEMKIGACGTVRENRSGYPRTQENSLSRTSERGSMRWIRQGPLLFVKWVDTKEVSICSTIHKAYTGDVVSRRQRQPDGTWKVREFHLPAPIKAYNKYMGGVDLSDQRLQNYTTRRKTYSWYKTFFFHFLDMAVVNAYLLYREQCEVKSRKPMSHLVFRKKLCEELCNAQLNSDPRKTIPMDHEVQRIQGGRRVCRQCKLEGRRSDTSWQCGRCDVPLCGSAPKNCFRKWHLPSPVKAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M0U2</accession>
    <name>A0AAR2M0U2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M0U2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052688.1">
        <property type="protein sequence ID" value="ENSPNAP00000080522.1"/>
        <property type="gene ID" value="ENSPNAG00000032169.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248530"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF30">
        <property type="entry name" value="THAP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="367" mass="42347" checksum="1F1DF83295200978" modified="2024-11-27" version="1">MCFVDLEKAFDCVPRGILWEVLREYGVHGSLLQAIQALYKQSRSLVRMAGSKSDFFPVRVGLRQGCPLSPILFIIFMDRISRRSQGMEGVRFGDLRVTSLLFADDVVLLGTSGRELQLSLDRFAAECEAAGMRISTSKSEAMVLTRERVESPLWVGDELLPQVEEFKYLGVLFTSDGTREREIDRRIGAGSAVMRALYRSVVVKKELRHKARLSIYRSIYVPTLTYGHELWVMTERTRSRIQAAEMSFLRRVAGLSLRDRVRSSVIREGLGVEPLLLHIERSQLRWFRHLVRMPPGRLPWEVSQASPPGRRPRGRPRTRWRDYIAQLAWERLGIPPRELVEVAGEREVWASLLRMLPPRPEPRRNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNT4</accession>
    <name>A0A3B4BNT4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RPL37A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNT4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001238"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012516.2">
        <property type="protein sequence ID" value="ENSPNAP00000001238.1"/>
        <property type="gene ID" value="ENSPNAG00000000609.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016988"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48188">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48188:SF5">
        <property type="entry name" value="RIBOSOMAL PROTEIN EL43-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="17958" checksum="150D95EC7C7DA51C" modified="2018-12-05" version="1">MNLIRSFFTKPRAHSEQHARPLQPRSSRRTSTSWSSHIPACNVHNDGKSRMRNFSSLFWLRTSPAKMAKRTKKVGIVGKYGTRYGASLRKMVKKIEISQHSKYTCSFCGKTKMKRRAVGIWHCGSCMKTVAGGAWTYNTTSAVTVKSAIKRLKEMKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSY0</accession>
    <name>A0A3B4BSY0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BSY0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002748"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009898.2">
        <property type="protein sequence ID" value="ENSPNAP00000002748.1"/>
        <property type="gene ID" value="ENSPNAG00000009101.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158066"/>
    <dbReference type="OMA" id="ANYILLM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF82">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="7">
        <location>
            <begin position="56"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="190" mass="21230" checksum="C82DF09607CE98C0" modified="2018-12-05" version="1">MAYHPADFTLGAVLGPPHPHPHPAAFSVLEQLESPIQLSSPSLQPAGAPDEPVVLLEGAELWARFHNIGTEMVITKSGRRMFPALKVRCAGFDKKARYILLMDIVTADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMSKVVSFQKLKLTNNISDQHGFVSRSAKHVRLLGSPDCWKARCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BU27</accession>
    <name>A0A3B4BU27_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cathepsin F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CTSF</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578216.1">
        <property type="nucleotide sequence ID" value="XM_017722727.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BU27"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002039"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011117.2">
        <property type="protein sequence ID" value="ENSPNAP00000002039.1"/>
        <property type="gene ID" value="ENSPNAG00000008537.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442600"/>
    <dbReference type="CTD" id="8722"/>
    <dbReference type="GeneTree" id="ENSGT00940000164681"/>
    <dbReference type="OrthoDB" id="387093at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000050">
        <property type="entry name" value="Cathepsin F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000004">
        <property type="entry name" value="Cystatin C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018639210" description="Cathepsin F" evidence="8">
        <location>
            <begin position="26"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="9">
        <location>
            <begin position="37"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="11">
        <location>
            <begin position="180"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="10">
        <location>
            <begin position="265"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53617" checksum="F80E4B9C69DC9754" modified="2018-12-05" version="1">MEVQLSRCHLVIACFVLAVVCSARALEDDLDRPLIPGAPGAPVRLSLTDPELMKAVKFAEERYNVGSNGLHIRRVSKIISASKQLVKGVRYSITVEIGRTQCKKSEVLDISEACEFFPESHKQKTEVCLFEVWDIPWQNKSTLLKQKCQPAVPKETPKIVRIPLSKDKPTKESLELLTMFKDFMVKYNRTYSSQEEAEKRLHIFQKNLKTAETLQSLDQGTAEYGVTKFSDLTEDEFRMMYLNPMLSQWSLQKKMKPAAPASKAAPESWDWREHGAVSPVKNQGMCGSCWAFSVTGNIEGQWFKKTGQLLSLSEQELVDCDKLDQACGGGLPSNAYEAIETLGGLETETDYSYTGHKQRCGFSTGKVVAYINSSVELPKEENDIAAWLVENGPVSAALNAFAMQFYRKGISHPLKIFCNPWMIDHAVLLVGYGERNGIPFWTIKNSWGEDYGEQGYYYLYRGSGLCGINKMCSSALVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVC4</accession>
    <name>A0A3B4BVC4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M20 domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PM20D2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVC4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002536"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010267.2">
        <property type="protein sequence ID" value="ENSPNAP00000002536.1"/>
        <property type="gene ID" value="ENSPNAG00000001236.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003365"/>
    <dbReference type="OMA" id="HYAITDT"/>
    <dbReference type="OrthoDB" id="6119954at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05672">
        <property type="entry name" value="M20_ACY1L2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000004">
        <property type="entry name" value="Peptidase M20 domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052030">
        <property type="entry name" value="Peptidase_M20/M20A_hydrolases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017144">
        <property type="entry name" value="Xaa-Arg_dipeptidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575">
        <property type="entry name" value="PEPTIDASE M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30575:SF0">
        <property type="entry name" value="XAA-ARG DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037226">
        <property type="entry name" value="Amidohydrolase_ACY1L2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="186"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="411" mass="45250" checksum="414DEB421679142B" modified="2018-12-05" version="1">MASVRGADGERLKHNICVCIEECREKLYGLSQDIWSTPELAFKEKQSHDLLVRFFSEAGGWTVEPSYILETAFRATWCLGTQKNLKVVNVGFLCEYDALPSLGHACGHNLIAEVGVAAAFGLKGALESQADVPVKITVLGTPAEEEGGGKIKLIQAGAFADMDVVFMAHPSQENKAFHPCICIRDCTVKYYGKASHAAAYPWEGVNALDAAVLAYSSLSVLRQQLRPDWRLHGIIKHGGTKPNIIPDYTELEYYMRTPLLKDLSYLKDKVETCFRSAAIATGCQVEITFPNPVYSNILPNPSLEKHYEENGRALGMEFKEECRSFTGSTDFGNVSFVVPGIHPFFYIGTEALNHTPEYATAAGSEKAQMYTLQTAKALAMTAADVLLNPTLLKQVKEEFTVAKCSQEQKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWI2</accession>
    <name>A0A3B4BWI2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC35E4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Putative transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TPT transporter family. SLC35E subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566725.1">
        <property type="nucleotide sequence ID" value="XM_017711236.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037403293.1">
        <property type="nucleotide sequence ID" value="XM_037547396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWI2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002854"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009724.2">
        <property type="protein sequence ID" value="ENSPNAP00000002854.1"/>
        <property type="gene ID" value="ENSPNAG00000009195.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435408"/>
    <dbReference type="CTD" id="339665"/>
    <dbReference type="GeneTree" id="ENSGT00730000111291"/>
    <dbReference type="OMA" id="HQDLEPC"/>
    <dbReference type="OrthoDB" id="10261634at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="9">
        <location>
            <begin position="24"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="331"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="395"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="553"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="695"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="347"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="596"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="633"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="731" mass="81400" checksum="27E80810CD3DD575" modified="2018-12-05" version="1">MIRSNDGASKREWRRARVPETLHLLSAVIVWLVTGTTISSLNKWIFAVYNFRYPLLLSALHMLTAIVVDYGLIKYWFVRRRGLDEHGLTSSAKFKVFLLSLTFCASIAFGNVGLNYVQLSFAQMIYTTTPIFTLAISSLVLGKQHHILKYTAMMPICLGASFSIMGEVQFDQTGCLFVFAATMLRGVKSIQQSILLQEEKIHSVFLLYLMAIPSFCILAVAALVLENWAGLQSPVHYDRHLWGFILLSCLGSVLYNLASCCVISLTSAVTLHILGNLNVVGNLLLSQLLFESQLSALSCAGAALTLSGMIIYQNSELIAGFLDARQARPAVASHERKTSTREAEPDSGSTSKTSSDMEPPLNQSLELSSEKCHTEEPWAELYNDLEPYLDLEPSLETSPILSPDLEPSPDASPSMYQDLEPSPPILSPDLELSPEASQKLCDNLVPCPETSPKLHNDIKLSPELYHNLETSPELHQDQETCAGNSLKLHNGLEFSLETSPELYHKSESSPEMHQDLKPCLETSPELHQDLEPCTEASVGLYRDLELLPETSPELHHELQPCPETSSEVHQDLEPCAEPSPELYNDLESLPETSPEVYHESEASPEVHQDLESCPETPPELYHDFPEASPGLYDDLRPAPETSPELHQDLEPCSETSPELLQDLELYPEASELLDDLPETSPAWYSDLELCPEPSQTCDDLKKAFPEPPELFPEPAPQADMWTEKQTHEKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYE9</accession>
    <name>A0A3B4BYE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017558063.1">
        <property type="nucleotide sequence ID" value="XM_017702574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYE9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004722"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006540.2">
        <property type="protein sequence ID" value="ENSPNAP00000004722.1"/>
        <property type="gene ID" value="ENSPNAG00000011133.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430230"/>
    <dbReference type="CTD" id="563626"/>
    <dbReference type="GeneTree" id="ENSGT00940000156410"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000008">
        <property type="entry name" value="Putative TBC1 domain family member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000005">
        <property type="entry name" value="TBC1 domain family member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000006">
        <property type="entry name" value="TBC1 domain family member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF15">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 12 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="564"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="411"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="491"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="858" mass="94112" checksum="9AB5C3199452FB49" modified="2018-12-05" version="1">MASHASPSSVDVVCAEREPPESRLLSVAGRWAPSATETEQGGVAGEAGPGCDRGAVNGGSSACVKTGLGREDRFGAEERCGEQEEVVNGFPEPSSSFVDLGGGQEDGRLSPATAGNMDGMAPPERDLGSSEVGDGTGAGVRVLEQETDPQLDCEQLPPAMACSCKNHRDSVCSQLTKKVPNGDVCMQIPRGCLDLERERMEGFLHRNAALDKGVRLGNCGFLVVNTRSLWDAPPGGPPRLGSPPENACSSPGSCDSPDQESGHASLEASDSTQPQQAASCEPDPGLDTLSELSGKLSISPLAENASDCAEDFSDAGFSARPQTLRTIPGCPVSLSCDATPLSPEDAADFFGDEGGVDGCLSAGSAARRQSAPDKLPEGICVDPDPSSDPKGTKRHGIAEFLTRSLFSWKTKEPRATAAQSAPGWRLFGKTPPKDNPPDSGTIQQECEARAGPGTGPHPRQPMRRKNLEFEPLSTTALILEDRPANLPAKSEEEAQRHRQEYNEMVAGAKRREMKEAQRKKKQMKERYRQEENIAHAMVIWNTEILPNWESMRNTRRVRELWWQGLPPSVRGKVWSLAIGNELNITPELYEIFLSRAKEKWRSFNETSSVSENEDCGASLADRESSLDLIKLDIFRTFPSLYIFQKGGPYHDLLHSVLGAYTCYRPDVGYVQGMSFIAAVLILNLEEADAFIAFANLLNKPCQMAFFRVDHDLMLKYFAAFEVFFEENLPKLFQHFQNNSLTPDFYLIDWIFTLYSKSLPLDVACRVWDLFCRDGEEALFRTGLGILRLYQDVLLQMDFIHSAQFLSRLPENTPAHTLFTCIANTQMLSNNRRWNQVFSALMKDGPKETDKTASPALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0L8</accession>
    <name>A0A3B4C0L8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myb-binding protein 1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MYBBP1A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYBBP1A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0L8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004435"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007017.2">
        <property type="protein sequence ID" value="ENSPNAP00000004435.2"/>
        <property type="gene ID" value="ENSPNAG00000010961.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017457"/>
    <dbReference type="OrthoDB" id="342531at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007015">
        <property type="entry name" value="DNA_pol_V/MYBBP1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213:SF2">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04931">
        <property type="entry name" value="DNA_pol_phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1165"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="640"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1174"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1215"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004435.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1265" mass="143053" checksum="91964CFA740FC19D" modified="2024-11-27" version="2">SETLIKGERRINGQITEADGPVRPAVSDPDGLLKRSRELLDLFWEIAKPERETRLQAAEKLVEQLKKSGEADELNYAVKRLVNGLSHTREQARTGYSATLAQVLSVFEEISLQSTLDQIKEKHNLQTANKKQIRNVVFGNFFGVLALSQSSRLHKETQVLLECIKLLQTLSQYREHLRELPRKTMVDILSETSEEVFEEVLLNALQTDLTCALSSPEQLELLLVAMQKFPSVIKPAKLKKLLGTANIISANTLPRLVEVLKTAARSVKKESVLPSVALDLLHVSLREDSFELFWKEAVISGLLSDPPGPCHYLVFRLLGVALPLLSVSQLQFVLSGEVMRRYGEHVASAQLPERFKFSPEMGEYVNSFMQSCREPERQLTVILAFTQLTHQGYPAVPSHWKVVEHMEPKALRQYIAWLKEAFCQPQLDKCLEFSTRKQREGQESTVPNETCVFRFRKWIVPRLTSIVENNRIKKDEELVMGIARFIFFHAFFDTKKATAGIPETTCSLSVPLDSTTRGITSSAFYSLLQHLNVLPVLDDSAEAGAGSKRRALGVMADGSMWVHRLAEFADALLKQDAHVQNTRTFTEEHTQAWDSMLQSVEMLMKKMKKSPAPEHTAFQHLFLIVGIQMFMAPEDSVELLKDLRSCMEKAQAKKSKKKKKEADCEQEPHWVEVVVEILLSLLSQPSRLIRNVCKTAFSRVCPHLTQPALSAILNIELDLKLCAGSSLHSHQRTEDITMKFIMTSHFINLCYQDEGESDSSDDDADDEAMEEEQEEVDQNFRLELMKVLQGQNELATEEDGGEDEQEMDDEAMMKLDKNLATLFGEQKKKLQAKKDEKERLRREKMLVRDFKIKVLDLVEVFLIKQGSSVLVLGMVEPLLSVIENGMSSESGQQEMDFLRRAAAIFRNQLCRGKQYCKEVEGREAELHEMLERLIGRAQKLRDSSVALYYFSAALYVVKVLRGVVKETDASQAAEVNMGKADVERVTNCFRDALTSFMTRRKSPLTGDMFIDLFNRFPAVCVNLLDTAVENITAGVREHQQSQACAMLLRALQSKDVKQLIPSAQWTKLCQKTAQQLIQCLQHVEHKNKAVHERTVKALELCQYLVRTIHTQKVEVNLDPLRDALRSMNADGSLQKAGQLEDTYWSVMKLFGIQKPKVEKVKKVVEEQTEQEAPKKKKKGFLPESKKRKNRKKPQVLEGKEATETAAGAGGVEGGKKKKKKKNKRKGQGAQSEAQSPNKKAKTEPPQSKKQKKKQKQAGGDQASAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C1B8</accession>
    <name>A0A3B4C1B8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tripartite motif containing 25</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C1B8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006968.2">
        <property type="protein sequence ID" value="ENSPNAP00000004464.2"/>
        <property type="gene ID" value="ENSPNAG00000010915.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000198821"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF14">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="211"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004464.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="45880" checksum="92C6183771502378" modified="2024-11-27" version="2">IDAKMSQPSGILEQELSCAICLQLYNDPVSLPCGHSYCLACVQTSQKSDLPGGQPRCPECRQEYDGLESLLRNFKLCGIVEGYRKAMSAGGFKMGKSVVPCDQCLDGTEPAVKTCLHCETSLCQGHLKKHQERHRSKSHALVEPLPDQDQRKCPDHRQEEYQHQLSNAQRSLYINLEPQLRQAANVTIPSLPAPQPTNTKRLRASLSTDTFRSELTQELQNLHSLMNPLDLSFNPATLHNSLILSMDLLTVKYWSGAKPSHSNGDQNERFTTAVQVMCSQGFAQGVHVWTVQLGPHCMWSVGLCYASIPRKGDHSKLGHNTLSWRLQWKNKKLTACYDSISTNVGDGSAVPPKRLEVALDYDGGSLAFYSITAKGRKLHLHTFRAAFKETVYPAFGLHSTSGESWITLMNTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C295</accession>
    <name>A0A3B4C295_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017561001.1">
        <property type="nucleotide sequence ID" value="XM_017705512.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C295"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005120"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005835.2">
        <property type="protein sequence ID" value="ENSPNAP00000005120.1"/>
        <property type="gene ID" value="ENSPNAG00000011420.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431990"/>
    <dbReference type="CTD" id="793956"/>
    <dbReference type="GeneTree" id="ENSGT00950000182979"/>
    <dbReference type="OMA" id="DTFTQHV"/>
    <dbReference type="OrthoDB" id="197676at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051145">
        <property type="term" value="P:smooth muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000002">
        <property type="entry name" value="Myocardin related transcription factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043451">
        <property type="entry name" value="Myocardin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793:SF5">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="RPEL" evidence="7">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="7">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="7">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="SAP" evidence="9">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="246"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="444"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="932"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="507"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="997" mass="108689" checksum="5D55D5F128A0F32C" modified="2018-12-05" version="1">MEAQGEAGIGGLLAPSPQSEAVTHEMEELSLQPSQNLPPLNERKNVLQLRLQQRRTREQLVDQGIMPPLKSPAAFHEQIRSLERARTENFLKHKIRSRPERAELVRMHILQETAAEPSLQATQLKLKRARLADDLNEKIAQRPGPMELVEKNILPVDSSVKEAIIDGQMQYPKTLPDVFDEDSGDAFSPEQPGSQESQGSAASPGEPKAMETTSPLPNNLLQHCSSAVQSTGNFLKSLSTNEKLVSPLAPQPQPVTTTAPSKPGPTLLKQSQPKLPGDKSRSKKSKEAKPRVKKLKYHQYIPPDQKQEPSEAPMDSSYARLLQQQQLFLQLQILSQQQQQHYNYQTILPAPLKPMIEGQNSSATVAISSGNSLPASIVVSLPTAAPPVRLNNTFTNRKAGTLPANLEEMKVAELKMELKLRGLPVSGTKTDLIERLKPYQESFAPSISSNSTGPNMQPCSTPMEVSSTTTSLSLGQQPPDALSSTPPVSPVPAEVHNREVALMDSQMEAQGQTGSASNQPLATVPEEQDRRLHEKERQIEELLRKLEQEQRLVEELKMQLQVEKRSGQTLAAAENNGSISPAALKAPVKMEKAVVPNCKVTSHTIKKLEESRPTQVTATALPQFIISHQGVSQVIGQPQTLLTTQHGGTQILLPVSLPNNATTIQLPNTNVKLQPILQAAVSPQSQGLIQSTQMQPAKAESSSVKQPVNHNHNIVQTLPVCSSGFQHSAIENQTGLEMAQCFLSSSPENTVSAQTSPITSSLTNDPLNKFPSQAQPAFILQPPAFSQAPKSRELPTYEDAVKQTRRLQAPAITQVPTATSQQMDDLFDILIESGEITPFSQPEHSVPKMMPVTANITTLPVNTALSRPPAQVQMAPPPALLVEPMPNLASLASDNQLEALLEGALVGEDEPEQRTLGILEELQSQLLEQPHSPMDTSELSFCDPPARSSSSSFSLQDTGLDNMEWLDLTMPGPIGPLNPLGIASDFLDTHDLQLHWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2R6</accession>
    <name>A0A3B4C2R6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PLCXD1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017576289.1">
        <property type="nucleotide sequence ID" value="XM_017720800.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017576297.1">
        <property type="nucleotide sequence ID" value="XM_017720808.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390689.1">
        <property type="nucleotide sequence ID" value="XM_037534792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2R6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006058"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004221.2">
        <property type="protein sequence ID" value="ENSPNAP00000006058.1"/>
        <property type="gene ID" value="ENSPNAG00000012131.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441321"/>
    <dbReference type="CTD" id="563489"/>
    <dbReference type="GeneTree" id="ENSGT00940000165420"/>
    <dbReference type="OMA" id="GDLWPHI"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08616">
        <property type="entry name" value="PI-PLCXD1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042158">
        <property type="entry name" value="PLCXD1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF131">
        <property type="entry name" value="PI-PLC X DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="32"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="325" mass="37120" checksum="A8CA2656BCAF6F7F" modified="2018-12-05" version="1">MSSTTLSEVSQTPLKDLPVDSWMSHLPNALWDIPLFNLTIPGSHNAITYCLDKNDRSPVDLTQPDMLQKLDKYMKPIIRPFVYKWAITQERSVREQLDCGVRYCDLRIAHRPNDSSSDLYFYHGVYTTVTVETVLKEIKAWLDAHPKEVVILSFSHFLGLSQELHTLLISTIKNVFDSKLCPKTEGVTLKNLWSLGYQLIVSYDNHAANCHRELWSHIPYWWANKCKAEALIEEFERRKNNGRPGGFFVTGINLTEDLKYICSHPTESLKDLVMSTYPTLLSWVREQKPGSNAGSLNIIAADFVTESQFIPTVIALNENLLSRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3K6</accession>
    <name>A0A3B4C3K6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 150 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID 150 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TAF2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556354.1">
        <property type="nucleotide sequence ID" value="XM_017700865.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3K6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005179"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005731.2">
        <property type="protein sequence ID" value="ENSPNAP00000005179.2"/>
        <property type="gene ID" value="ENSPNAG00000001742.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429242"/>
    <dbReference type="CTD" id="6873"/>
    <dbReference type="GeneTree" id="ENSGT00390000000420"/>
    <dbReference type="OMA" id="EQPDYQW"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000003">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000005">
        <property type="entry name" value="transcription initiation factor TFIID subunit 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="10">
        <location>
            <begin position="515"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="11">
        <location>
            <begin position="635"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1046"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1141"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1145"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1177"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1191"/>
            <end position="1209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005179.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1209" mass="138552" checksum="477CFDED6B49E598" modified="2024-11-27" version="2">MNRKKDKGFESPRPYKLTHQVVCINNINFQRKSVIGFVELTIFPTVVNLNRIKLNSKQCRIYRVRVNDLEAPFIYNDPTLEVCHHESKQRNLNYFSSAYSAAVSAVDPDAGNGELSIKVPSELWKQGDEMKVLKVYIEFSLDQPKGGLHFVVPDVEGSMAERGAHVFSFGYQNSTRFWFPCVDSYSELCTWKLEFTVDASMVAVSCGDLVETVYTHDMRKKTFHYMLAIPTAAPNISLAVGPFEILVDPYMHEVTHFCLPQLLPLLKHSMSFLHEIFEFYEEILTCRYPYSCFKTVFIDEAYVQVSSYASMSIFSTNLLHSGMIIDQTPLTRRCLAQALAQQFFGCFISRMSWSDEWVLKGISGYIYGLYLKKTFGVNEYRHWIKEELDKIVDYELKTGGVLLHPTFTGGKEKENPTPHLHFSIKHPHTLSWEYYKMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKYQSHMWSQMLLSTSGFLKSISNVSGKDIGPLIKQWVDQSGVVKFFGSFAFNRKRNVLELEIRQDYTSSGTQKYVGPIKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNKKKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSILRKVEFEQADFMWQYQLRYERDVVAQEEAISALEKFSTAASRLALTDILEQDQCFYKVRMQACFCLAKIANAMMSTWTGPPAMKSLFTRMFCCKSCPNIVKTNNFINFQSYFLQKTMPVAMALLRDVHNLCPKEVLNFILDLIKYNDNRKNKFSDNYYRAELIDALTNSLTPAISINNEVRTVDSLNPDVRLILEEITRFLNMEKLLPSYRNTITVSCLRAIRMLQKNGHIPSDPSLFKSYAEYGHFVDVRIAALEALVDYTRVDRSADEFQWLLEMVQNDPLHYVRHKILSMLAKNPPFTKAAESSLCNEALVDQLWKLMNSGTSHDRRLRCDAVNLYHTLFGLTRPSCLPLPELGLVLNLKEKKAVLNPTIKPEQGSGGPELPPAVVFTSSVSSQETLEEEHVVMEPPLGGMVLPSQGLKRKAETPLASPPEPGQVLQEEPVVKGGVKSRFPTQEEEDIDMDTVHDSQAFIYHHLNMLDRPSTPGREPPTMEQTIMSMPATPVPLFSKESTLSLKHGGEHHHHHHHHHHHEHKKKKKKHKHKHKHKHKHDSKDKDKEKERDSHTFSNSPASGRSMRSPSLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6E3</accession>
    <name>A0A3B4C6E3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 72 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Transcription factor-like 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Deposition-and-exchange histone chaperone specific for H2AZ1, specifically chaperones H2AZ1 and deposits it into nucleosomes. As component of the SRCAP complex, mediates the ATP-dependent exchange of histone H2AZ1/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS72/YL1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577187.1">
        <property type="nucleotide sequence ID" value="XM_017721698.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393148.1">
        <property type="nucleotide sequence ID" value="XM_037537251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C6E3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006129"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004093.2">
        <property type="protein sequence ID" value="ENSPNAP00000006129.2"/>
        <property type="gene ID" value="ENSPNAG00000012209.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441926"/>
    <dbReference type="CTD" id="795272"/>
    <dbReference type="GeneTree" id="ENSGT00390000017503"/>
    <dbReference type="OMA" id="MSFVQQR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046757">
        <property type="entry name" value="YL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 72 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275">
        <property type="entry name" value="YL-1 PROTEIN TRANSCRIPTION FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05764">
        <property type="entry name" value="YL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="6">
        <location>
            <begin position="312"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006129.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="380" mass="42738" checksum="A7EADA56C4D779DA" modified="2024-11-27" version="2">MSLAFCRAPRKNAGSRMSKLLDAEEEDEFYKTTYGGFNDESGDDEYKGDLSDTEDEVDSDFDIDEGEEPDSEQEEDGPRRKSRVVTKAYKEPIKAVKPKPKPKKLSELPRRVDKIKTEKHKTLELQDDISKNRKSVRQSTTEHTRLTYLRLQERQVAPRRKKGTRHERPLTQAELLAEAKVTAEINLRSLENYERLEADKKKQVHKKRQCVGPVIRYHSVLMPLVSDPSFKEENVDVEGLDQDPQQSLPGSGSGAAVMSQSEATLSGQPVSISAPPPPGGGRCSRTYITFSDEDTFQSYFPKTSAPRVPVKEVCPVTHKPALYRDPITDIPYANVRAFKIIREAYKKYVAAHGLPCPGTTLPAEPTAKSLRQKIVIKQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAV9</accession>
    <name>A0A3B4CAV9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">VDAC2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAV9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007564"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001616.2">
        <property type="protein sequence ID" value="ENSPNAP00000007564.1"/>
        <property type="gene ID" value="ENSPNAG00000002714.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00540000073890"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044291">
        <property type="term" value="C:cell-cell contact zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098636">
        <property type="term" value="C:protein complex involved in cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042974">
        <property type="entry name" value="JAM-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44598:SF3">
        <property type="entry name" value="JUNCTIONAL ADHESION MOLECULE 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44598">
        <property type="entry name" value="JUNCTIONAL ADHESION MOLECULE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017460122" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="133"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="368" mass="40144" checksum="CDC670B71A59A72F" modified="2018-12-05" version="1">MAVQAVLLLILMSVSLGNTQTEMLKLECQTAVGVVGQTTKLCCSFKKNFKADQINITAGFVTKRGERDPLFWFKKGVANGDHRFKPPSLNDPSLLLTDTAVSDEGQYDYTVLTNRGITKEALRINVTAKYNPPSVSTWPEKIEEGSPAELNCIASGGYPAGTIQWFDSTNTNWTMNATLEITEREDQLLHLSSKLTFAKINSSWAPFKCVVLNSKFVKDGETTSHQSHTGDEDRSGDTGFIGGSVIAGLIVIGLIIVGLLLALLYRRRHVQRDFPGITRQAGADADADIDADEVGSLHAPVKHKGFYLHRFSHALSDGSVPVVAFKIPSSNLGLSGLYVSSPIHARESLNHLLCCLCCRSSAEAKWRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CCB1</accession>
    <name>A0A3B4CCB1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">OBI1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CCB1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008516"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034949.2">
        <property type="protein sequence ID" value="ENSPNAP00000008516.2"/>
        <property type="gene ID" value="ENSPNAG00000003032.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013512"/>
    <dbReference type="OMA" id="PEAECGT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039209">
        <property type="entry name" value="OBI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14609:SF1">
        <property type="entry name" value="ORC UBIQUITIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14609">
        <property type="entry name" value="RING FINGER PROTEIN 219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="495"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="562"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008516.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="577" mass="63232" checksum="DBA60D90F66F8026" modified="2024-11-27" version="2">MAQSCQSVTLSLTLPIACQICLGKVRKPKICSNSHVFCSTCIDVWLQKCSQCPTCRVAVTPENPCREIIGATNDSESNENHSVKRRLRKTRGELILQEYEDEIETLLKENEDLRSKNLSLETQLKTALEPSTLFVAQTETEAVDPSLLQESAKKLKAANELYQKVKQDVEKLKEANKTLRSQNVDLIQENMRLKAEVESRSPQKFGRYTVAALEAKILQQEREMTQLKKALERSDKYIEELEAQVSKSRREGANTTEDSQSKGSVSGGTQAAGKTAHYRVCSSNIFLQPPLCSPATRSLSEMEEASVCTDLERKSINLPNSHGFLLTTSDTCRGIGFLNEALSPRNDALQNITVPVPSTPSSAFSSLSLRSPAVCSERKPSFKRLTYLRRLSFDDCSSSSSFSASASVDQSSKAGQVDKDGSFSHRNVLNTGKEALWSGWQDIKPASFLSANENSAGFKQRESEDGSSVDEAEASEASMDLAYLDKISELDSMMAEGEPSHHSPLLSHPPACNAPLTPKLEAHLDVLADPGSVRKQQRTSDAQDGSRNGPECSGESTKRKCSVSLASASPSKMSKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CE67</accession>
    <name>A0A3B4CE67_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphatidylinositol transfer protein beta isoform</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PITPNB</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:38572"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an N-(acyl)-sphingosylphosphocholine(in) = an N-(acyl)-sphingosylphosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:43776"/>
            <dbReference type="ChEBI" id="CHEBI:64583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43777"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CE67"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008769"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014913.2">
        <property type="protein sequence ID" value="ENSPNAP00000008769.2"/>
        <property type="gene ID" value="ENSPNAG00000014374.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155101"/>
    <dbReference type="OMA" id="NPECPRM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008525">
        <property type="term" value="F:phosphatidylcholine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035845">
        <property type="term" value="P:photoreceptor cell outer segment organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08888">
        <property type="entry name" value="SRPBCC_PITPNA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000004">
        <property type="entry name" value="Phosphatidylinositol transfer protein alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF27">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN BETA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="16">
        <location>
            <begin position="3"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008769.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="264" mass="30840" checksum="AACCD5AEDD2A2F98" modified="2024-11-27" version="2">MCRYQVGQLFSVAEASKNETGGGEGIEVLTNEPYEKEGEKGQYTHKIYHLKSKVPAFLKMIAPEGALVFHEKAWNAYPYCRTSRFNEYMKDDFMIKIETWHKPDMGFMENVHELDPETWKTVEVVPIDIADKSQVEPGDYKPEEDPALYHSEKTDRGPLGPDWKSELAAKEDAPRMCAYKLVTVKFKWWGLQSKIEQFIHRQEKRIFTNFHRQLFCWIDSWVELTMEDIRRMEAETQKELEESVSNQVQISGTTFSHGVFAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGE8</accession>
    <name>A0A3B4CGE8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">HCN3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570856.2">
        <property type="nucleotide sequence ID" value="XM_017715367.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGE8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017890.2">
        <property type="protein sequence ID" value="ENSPNAP00000011062.2"/>
        <property type="gene ID" value="ENSPNAG00000016712.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437924"/>
    <dbReference type="GeneTree" id="ENSGT00940000154743"/>
    <dbReference type="OMA" id="WLVTLGM"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000002">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF7">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="9" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="513"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="64"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011062.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="643" mass="73617" checksum="A7AD2008EF898930" modified="2024-11-27" version="2">MDGKGAGEELGSEERPAPNGDSRQRGRFSLPGWRSSSQSSVNGERSGDLSRRLLSLVNRYDDQDGTVADLNSKPSEVTVGSEGPTPTTGHRTSTASSSGKDMPDTTGPGGVKFTPDFEDYDSIDQSMNLQKQLSSMLQPGVNKFSLRMYGSAKGVAAEQERVRSFGVWIIHPYSDFRFYWDLVMLFLMMGNLVILPWGITFFEDQNTLPWMIFNMASDTLFLTDLVLNFRTGILEGDNSHIILDPQVIRRRYLQGWFLVDFISSIPVDYIFLAVDLEARVESAEVYRTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFMVPMLQDFPSNCWVSKNNMVNATWEVQYSYALFMAMSHMLCIGYGAQAPERLTDVWLTMISMIIGATCYAMFLGHATNLVQSLDASHRQYQEKYKQVQQYMSFHKLPADMRQRIHDYYEHRFQGKMFDEENILGELSDPLKEDIVSYNCRGLVANMPLFGNADPHFVTVLLTKLRFEVFLPGDIIIREGTLGRKMYFIQHGCVTVITREKKEKKLSDGCYFGEICLLTRGRRTASVRADTYCRLYSLSVDSFNEVLEEHPIMRRAFESVAIDRLDRVENKITSNTTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CH27</accession>
    <name>A0A3B4CH27_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">PRKD3</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Activated by DAG and phorbol esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CH27"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010131"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016718.2">
        <property type="protein sequence ID" value="ENSPNAP00000010131.2"/>
        <property type="gene ID" value="ENSPNAG00000015710.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183024"/>
    <dbReference type="OMA" id="PKVPRDC"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
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        <property type="entry name" value="Serine/threonine-protein kinase"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015727">
        <property type="entry name" value="Protein_Kinase_C_mu-related"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D3"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="15 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="157"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="286"/>
            <end position="336"/>
        </location>
    </feature>
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        <location>
            <begin position="432"/>
            <end position="551"/>
        </location>
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    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="593"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="344"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="716"/>
        </location>
    </feature>
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        <location>
            <begin position="599"/>
            <end position="607"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 22">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        </source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="914" mass="103009" checksum="43A768001B50DE81" modified="2024-11-27" version="2">MSACSSTTPLTPLPKAYLHSQHPPTPPTPSPSPSPPVALYHRLSNGSHSAPSPTLSRGSLHGVSFLLQIGLTRETVNLEAPDLSLNAVKDLVCSIIDQKFPECGFFGMYDKILLFRHDLNSDNILQRLTSAEDIHEGDLIEVVLSAQATVEDFQIRPHALYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPSLSVPRPLPAEHSVVVKIIFPTLFTPVNPQQQRSHQEAGKRIPSWSGRPIWMEKMVLGRVKVPHTFVIHTYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPKDCLGEVLFNGEPASPGPDSDTTMEVDSSDVNSDSSRVLDDSEEPTTPDDKIFQGYPFTDQESNEEAVKTPQISPSTSTNIPLMRVVQSIKHTKRRSSTVVKEGWMVHYTSRDNLRKRHYWRLDSKSLTLFQNDSGAKFYKEIPLSEILQVEAAQDFGSLALGSNPHCFEIITATMVYYVGENMGGPHLHIHNPMLAASGVGLEVAQSWEKAIRQALMPVPVTPQPSVGSAAGQEDLSISISVSNSQIQENVDISSVYQIFADEVLGSGQFGIVYGGKHRKTGRDVAIKVIDKMRFPTKQESQLRNEVAILQNLHHPGIVNLECMFETPERVFVVMEKLHGDMLEMILSSEKSRLPERITKFLVTQILVALRHLHFKNIVHCDLKPENVLLASAEPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRSKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDINDQIQNAAFMYPPMPWKEISTEATDLINNLLQVKMRKRYSVDKSLSHPWLQDYQTWLDLREFETRRGERYITHESDDARWEEYADEHSLIYPKHFIMAPNLDDMDEDPYVLSSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CH75</accession>
    <name>A0A3B4CH75_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptor 22</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CH75"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009824"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035463.2">
        <property type="protein sequence ID" value="ENSPNAP00000009824.1"/>
        <property type="gene ID" value="ENSPNAG00000004908.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234518"/>
    <dbReference type="OMA" id="MKKGQRF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000084">
        <property type="entry name" value="Probable G-protein coupled receptor 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000116">
        <property type="entry name" value="probable G-protein coupled receptor 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF127">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="63"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="429" mass="47046" checksum="3BC56D2AD3CECF29" modified="2018-12-05" version="1">MGTESYTHQLETSDGAGTLASITALEGQMGASAATGWYMPYPVSFQVSLTSVLLLELVLGFSSNLTVLVLYCSQSNLVDSVSNMVTVNLHVLDIVVCVLCLPLTVVIVLLPTGRNLALICCFHEACVTFTSISTAINVLVISLDRYDISVRPANRFLTPRWAALLLAAVWAVSLAVFFIPFIEVDFLSSEPDNGMADNSLNPAWRNRTLLCVGGQGYHTGLGMYYHILLQVPIFFATLVVMLFTYSRILRALNIRIATSPPPMSSTPLVNASTATAPAPAVGVQASVSAIIALRRAVRRHRDRRERQRRVFRMSLIIICSFLGCWAPISVANILILAFGPSDGLVRARLWFLAMAYGTTISHPLLYAFTRQKLRRALRAKVKKRVVSLLQVDPSPGGTVIHNSWVEPRQGRKLRLEGSDATDRCLTEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIM8</accession>
    <name>A0A3B4CIM8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Fanconi-associated nuclease</fullName>
            <ecNumber evidence="18">3.1.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">FAN1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Nuclease required for the repair of DNA interstrand cross-links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.</text>
            <dbReference type="EC" id="3.1.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the FAN1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.1" evidence="18"/>
    <dbReference type="RefSeq" id="XP_017559706.1">
        <property type="nucleotide sequence ID" value="XM_017704217.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIM8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011220"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018108.2">
        <property type="protein sequence ID" value="ENSPNAP00000011220.1"/>
        <property type="gene ID" value="ENSPNAG00000016852.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431206"/>
    <dbReference type="CTD" id="22909"/>
    <dbReference type="GeneTree" id="ENSGT00390000018637"/>
    <dbReference type="OMA" id="ECRVESM"/>
    <dbReference type="OrthoDB" id="76364at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004528">
        <property type="term" value="F:phosphodiesterase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22326">
        <property type="entry name" value="FAN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000004">
        <property type="entry name" value="Fanconi-associated nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033315">
        <property type="entry name" value="Fan1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049132">
        <property type="entry name" value="FAN1-like_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049126">
        <property type="entry name" value="FAN1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049125">
        <property type="entry name" value="FAN1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049138">
        <property type="entry name" value="Fan1_SAP_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014883">
        <property type="entry name" value="VRR_NUC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15749">
        <property type="entry name" value="FANCONI-ASSOCIATED NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15749:SF4">
        <property type="entry name" value="FANCONI-ASSOCIATED NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21315">
        <property type="entry name" value="FAN1_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21169">
        <property type="entry name" value="Fan1_SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21170">
        <property type="entry name" value="FAN1_TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08774">
        <property type="entry name" value="VRR_NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00990">
        <property type="entry name" value="VRR_NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00734">
        <property type="entry name" value="ZnF_Rad18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51908">
        <property type="entry name" value="ZF_UBZ4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 17 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14 17 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 18" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBZ4-type" evidence="20">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="90"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="288"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU365033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="995" mass="111770" checksum="2AE10F6FA444ABE0" modified="2018-12-05" version="1">MDSRAGRNQTKGHARPLKRLSLAKNDGQRGKAKENTSNKQKGLSITSFFKNTPPSKLACPLCGKLIPRYKINEHIDSQCRDFLEENGLEASSASHNKQEKTTCPLPNNVPRSPDKKMDASFDKNNQGTKTSPYFKKNCSAKQESPKASDQDKVVKTVSLGSLSSKLSRRALKFSENANPPAEAEPNCSELSTSQKENCDENVSIESTQTFPSEVATVNLPQVESTHRLKETAPKVESLSAEASQSVKTAAMFRLMKRRKEESPETCAASNLKKRSRYAGYKNKNATFPVVSHSQRSHVDSPFEVSKSKLEDVQRDEITPPHKSRGHPQEPSEVSVESGSPRRPYYLQNFLTILEAVLENKDDMMLFNEDDLSSVHGFKQLSVPGQMLYVRLFQRKLKWLQVSKLDYSEIGSDLRPVVQELVCSGFLQTESELKDVQEVLDLLPAPELRSLAKTFHLGGPGNSTQKQQLVEGLLRLAKQRSLFALAPAQNSTGAVILKRAKQLAGSCVRLSRGPRAVFSRVLLLFSLTDTLEEEEMAAGGQGQLYTILLVNSGRLAFPVYTVQRSAQVFRDRDDLIRYEAAMRTLQEVITAMQAGSWEDAHVLYSTAKATWQDLKQTCDWSCHEALPVFLRCFTVGWTYTRVLSRGVEILQRLRRYEDAVEELRSLLSQTVFCPDSRGRWWDRLALNLHQHLKQHEQAIYAIRDGLMDPLVRTGHRLSLHQRAARMNESASFKKYRLLLRKLPSLHVQDVTHVTIRGQLFPHEGGMGKSVFLRPATGDEDSEEGDNAMVICSVEELALAHYRKLGFEQGIHGEGSTFSTLFGLLMWDIVFMEGVPDVFRTPYQTCPLDFHTDSFYGNRQEAIEARAELLREASTETLQELLAEVWNSQEGRVCALVNWERFSSLQQAQSLVACLGGHFLSGVVIRMAKDYRHSRGGLPDLVVWSTSTNSYKLVEVKGPNDRLSQKQQLWLEELQKLGADVEVCHVTATGARGTHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJL0</accession>
    <name>A0A3B4CJL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ENOX2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ENOX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576976.1">
        <property type="nucleotide sequence ID" value="XM_017721487.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036174.2">
        <property type="protein sequence ID" value="ENSPNAP00000011573.1"/>
        <property type="gene ID" value="ENSPNAG00000017230.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441782"/>
    <dbReference type="CTD" id="10495"/>
    <dbReference type="GeneTree" id="ENSGT00390000006788"/>
    <dbReference type="OrthoDB" id="10039782at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:ultradian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="RRM_ENOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative ecto-NOX disulfide-thiol exchanger 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ENOX"/>
    </dbReference>
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        <property type="entry name" value="ENOX1/2_dom"/>
    </dbReference>
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        <property type="entry name" value="ENOX_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
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        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16001">
        <property type="entry name" value="ECTO-NOX DISULFIDE-THIOL EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16001:SF7">
        <property type="entry name" value="ECTO-NOX DISULFIDE-THIOL EXCHANGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23267">
        <property type="entry name" value="ENOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="172"/>
            <end position="251"/>
        </location>
    </feature>
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            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
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            <end position="499"/>
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            <end position="576"/>
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            <end position="371"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="560"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJY4</accession>
    <name>A0A3B4CJY4_PYGNA</name>
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        <recommendedName>
            <fullName evidence="3">Stathmin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">STMN3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1 3">Belongs to the stathmin family.</text>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="181" mass="20665" checksum="7BDC53A91C364428" modified="2018-12-05" version="1">MASTVSAYSEKLMEMSVLSLICSCLHSKPHPQTLGLFEDMEVKSLNKRISGQAFEVILKSPTDLSPDRPQSLSLAAHKKEPSLGELQKKLEAAEERRRSQEKQILKQLAEKREHEKEVLNKAQEVNNNYSKMAEEKLNHKMEVSSENRTARLSALKQRMREKELHAAEVRRNKELQSDLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CM30</accession>
    <name>A0A3B4CM30_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CM30"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013092"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036817.2">
        <property type="protein sequence ID" value="ENSPNAP00000013092.2"/>
        <property type="gene ID" value="ENSPNAG00000018930.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="OMA" id="RNCECCH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043579507" description="Ig-like domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="24"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="134"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013092.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="282" mass="31419" checksum="B5A1D77CC8B61CE1" modified="2024-11-27" version="2">MCGVIFAVGLFFQILTLIKYEFHPFKEHSFISVNTGENVTLSCKVEKSDTYVQFWYKQQLWQKPKEVVSKLDQKPPFILNGSKFSVNEDFSLTIKETSEDDEGMYFCGKTDGKTFTFSNIKVLAVTGNHITLNISVLQTPVRGLVSPGESVTLQCTITAADLRVLWFRAAAGQSFPEIIYTHQNSSSRQCEISSSTSSSLYEFSKNILDHIHTGTYYCAVAACGKIIFGNGTSVELSKSFCALLGACVADGKCFLGLELYFHVYVIKIKKNIKNTMCSVLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMJ6</accession>
    <name>A0A3B4CMJ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AT-rich interactive domain-containing protein 3</fullName>
            <shortName evidence="4">ARID domain-containing protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RBM10</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer with ARID3A. Interacts with unphosphorylated RB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMJ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012046"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019189.2">
        <property type="protein sequence ID" value="ENSPNAP00000012046.2"/>
        <property type="gene ID" value="ENSPNAG00000017822.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156052"/>
    <dbReference type="OMA" id="FMGKGMA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023334">
        <property type="entry name" value="REKLES_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF30">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51486">
        <property type="entry name" value="REKLES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="REKLES" evidence="6">
        <location>
            <begin position="362"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012046.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="477" mass="52135" checksum="14F95443241440AB" modified="2024-11-27" version="2">MERKERRLREAHIMYAQQVAAQQAILAAARASGVAQGQTRADSERDNKERRGQDSDDEDDDNDDAADDDDNEEEMMEGDEASEEEAGAVSGLEFLRKQTLALQQGAARLQGHSFGSYTGTPAPAPGTQREPSPPVQVKQEPEDGLSPVEPASAASPNGQADWSYEDNFKQNGVGVWGEESDSSRGRGEASRDFAKLYELDSDPNRKEFLDDLFTFMQKRGKMFPSDWLDVYALEDGGPAETCGLACGQETSLLLNITKCAYAVIIMYMKYLYPYECARKGLSSPNELQAAIDSNRREGRRPSYSSSGLFRFSPSPSTASHILSPPKMHLAALDASPSLLAARAPSLALALGPQQVAGLARAATLEQLREKLETGVGAEGPERKMARLAEEQQRLMQQAFQHNLLAMASQMPMNLRLSMPPREDKHDLAMSISSSGAASISVSVEVNGVLYSGTHHIFPSQGQQNFKDIIPQRSKAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNG7</accession>
    <name>A0A3B4CNG7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CD99 antigen-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CD99L2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
            <orientation evidence="10">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD99 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037402731.1">
        <property type="nucleotide sequence ID" value="XM_037546834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNG7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012885"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036701.2">
        <property type="protein sequence ID" value="ENSPNAP00000012885.1"/>
        <property type="gene ID" value="ENSPNAG00000018684.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434648"/>
    <dbReference type="GeneTree" id="ENSGT00940000154344"/>
    <dbReference type="OrthoDB" id="8961553at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022078">
        <property type="entry name" value="CD99L2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076:SF12">
        <property type="entry name" value="CD99 ANTIGEN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076">
        <property type="entry name" value="CD99/MIC2 PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12301">
        <property type="entry name" value="CD99L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017236154" description="CD99 antigen-like protein 2" evidence="14">
        <location>
            <begin position="25"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="30"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="297" mass="30811" checksum="2EBBAA8F1214BEE9" modified="2018-12-05" version="1">MRTLSAAAWTLLALLSLLAVKGLGQDLDLSDAFDDLSPSPTPKPKPKEPAGGAGTGGDLELSDVLDDEPTPKPPVQPKVPVKPKPKPEETHSNFFAPTVRPALLPRTTIRPLRTSAPPRKVQDDFEDLELIDALDPSNDIGGKGKDPSKGHKSVGGGGGRGGGRPNIRGGNGGGTFTDEDLLDVGNSEGYKPDPSKGGRGAAGSGDLDSTDNGDYDTMAETGTIAGIVSAVAMALVGAVSSYISYQKKKFCFSIQQSLNADMVKADNPEAVVAQEPQVQQTLLQPPNAEPPAEDNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CR11</accession>
    <name>A0A3B4CR11_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017576886.1">
        <property type="nucleotide sequence ID" value="XM_017721397.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017576888.1">
        <property type="nucleotide sequence ID" value="XM_017721399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CR11"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021477.2">
        <property type="protein sequence ID" value="ENSPNAP00000013793.1"/>
        <property type="gene ID" value="ENSPNAG00000019683.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441718"/>
    <dbReference type="CTD" id="100332162"/>
    <dbReference type="GeneTree" id="ENSGT00390000004923"/>
    <dbReference type="OMA" id="YYNLHRA"/>
    <dbReference type="OrthoDB" id="311172at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000117">
        <property type="entry name" value="Fibronectin type III domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052120">
        <property type="entry name" value="FNDC_type_III_4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017263148" description="Fibronectin type-III domain-containing protein" evidence="10">
        <location>
            <begin position="21"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="27"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="218" mass="24366" checksum="00FE2719035F1654" modified="2018-12-05" version="1">MRVFLTVCLVLFLFSCFCLAGANRPAAPVNVSVTLLRARSPTVSWNVLEGEAVIGYAISLQRQDGLMQRFIREVNTTSRACVLWDLDENTDYIIQVQSIGLHGESQASKRVHFRTLEKSDHFQSSMVAQGDPSVEGLDISRSLLTEEMVIIMTVLLMWAAVIALFCRQYDIIKDNDSNNHSKEKTKPLSEHSTPDYRNGGLLGSKFQRTPSSVSIIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTX0</accession>
    <name>A0A3B4CTX0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-microseminoprotein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-microseminoprotein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTX0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037486.2">
        <property type="protein sequence ID" value="ENSPNAP00000014845.2"/>
        <property type="gene ID" value="ENSPNAG00000020831.2"/>
    </dbReference>
    <dbReference type="OMA" id="ENCVAIF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1900">
        <property type="entry name" value="Beta-microseminoprotein (PSP94) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008735">
        <property type="entry name" value="PSP94"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10500">
        <property type="entry name" value="BETA-MICROSEMINOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10500:SF7">
        <property type="entry name" value="BETA-MICROSEMINOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05825">
        <property type="entry name" value="PSP94"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014845.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12202" checksum="37EA1B066195D278" modified="2024-11-27" version="2">MVRQQNFLKYFVLIFPDNFVVHIFLFLKNTPFLGQTNCIDETDGSEHGMDSTWTNSQCYKCHCSSTGMTCCDTIVPPSNPNPTTCTVVYDYQTCQANLVSRDDPSLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTY8</accession>
    <name>A0A3B4CTY8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CART family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567796.1">
        <property type="nucleotide sequence ID" value="XM_017712307.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTY8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023777.2">
        <property type="protein sequence ID" value="ENSPNAP00000015557.1"/>
        <property type="gene ID" value="ENSPNAG00000021591.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436081"/>
    <dbReference type="GeneTree" id="ENSGT00390000018319"/>
    <dbReference type="OrthoDB" id="8911843at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008343">
        <property type="term" value="P:adult feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032099">
        <property type="term" value="P:negative regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.30">
        <property type="entry name" value="CART, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009106">
        <property type="entry name" value="CART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036722">
        <property type="entry name" value="CART_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655">
        <property type="entry name" value="COCAINE AND AMPHETAMINE REGULATED TRANSCRIPT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06373">
        <property type="entry name" value="CART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64546">
        <property type="entry name" value="Satiety factor CART (cocaine and amphetamine regulated transcript)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017414472" evidence="5">
        <location>
            <begin position="28"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="105" mass="11990" checksum="897BDAE5FA0975C7" modified="2018-12-05" version="1">MTRAEGISLTLWVFLLIIISRATLIDASSEKSGDQSEEKRELLGVLQKVLEKLQNRRTVTWERKHSRLPECNIGDFCTVKKGSRFGQLCDCPRGSKCNLFFLKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D497</accession>
    <name>A0A3B4D497_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MLH3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000019167"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028807.2">
        <property type="protein sequence ID" value="ENSPNAP00000019167.1"/>
        <property type="gene ID" value="ENSPNAG00000004915.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014380"/>
    <dbReference type="OMA" id="FVCKANE"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002049">
        <property type="entry name" value="Si:ch73-170d6.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052090">
        <property type="entry name" value="Cytolytic_pore-forming_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048997">
        <property type="entry name" value="Stonustoxin-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040581">
        <property type="entry name" value="Thioredoxin_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31594">
        <property type="entry name" value="AIG1-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31594:SF16">
        <property type="entry name" value="SI:CH211-281L24.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21109">
        <property type="entry name" value="Stonustoxin_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18078">
        <property type="entry name" value="Thioredoxin_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="516"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="611"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1162"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1293" mass="146096" checksum="FBF84612532906CC" modified="2018-12-05" version="1">MSDSKTFELAVLGRPFQLGMLYDCRRDALIPGITLWDSETLMRNVNVRPQPNTEFKIIASDSSEEKLSALNVNASLEASFLCGLVSVKGSAQYLNDKKKSTRQSRVTLQYKSTTRFEQLTMEHLGIGNVKHCNVFEEGSATHVVTALLYGAQAFFVFDQEVSSNESHQEIHGNLQACIKNIPTISIEGQASLDMTDDENQRTNKFTCTFHGDFALESNPVSYQEAIKVYSQIPKLLGENGEHAVPVSAWLYPLRKLDTAAAQMVREISVGLVRGAQRIMDELDDADMQCQDLMKVDMAIQFPEIKAKLRKFKSLSSEYKLVFQKKLSKLLPSIRGGGKEEQDLVTLLTSKERSPFQSVLMSAYLNDREREMNIIRSYLEIMREVPVLSSSKDLDKAVLKANTDYVVAFAFSSLKEEESYLLDLENYLKEVSRSGEEHFSYEATAPKKSEQWFCSKDVSALTRQNIQLFLDFKRSNNGRQNIDFFIVSIPDNHVTASSIHVYEKGICLSSEFELPSEPHVPSFLRAEHDQIHLQIKPPAHGISSVESYCVSHQTVQSSDWTEVTTEGNSLSVTIKQLEPNEEYRFKCKAVCRPGVSLSSEETAFFKTHPCGPPGPLKLKSKESDKITITWDMPTSIGKDIQVIGYVVEFQEQPKKEQIAEVWESIRIANRQCTIQGLRADTDYRIRVLANCAPAGMSLPTPESMFTTTKLSDKSVQMKSAEASSQLFLEKSFLLKKGNPSIYVLNQEKKFRENEQFNQYAFGRKVEQGQNKVLLLLGSTGAGKTTLVNAMVNYILGVKWQEHHRFKLIHEVTNRTQAESQTSEVTSYELYNQPGFQVPYSLTIVDTPGFGDTRGMDQDKLIIEKMKSFLCSPLGINHINAVCFVVQASLARLSAAQKYIFDSVLSIFGKDVAENIIILVTFADGKDIPVLQAIKAAGLPCHKDSKGSPTHFRFNNSVIYTQKKVKNTSESDSGEDDAEEEDDEKLKEIVWTSNFKQMSKFFIKTLGVLNSKDLTLTKEVLEERERLEKAMADLTPQITAGLSMLNRIKAFKEGLKSESSNMKQNENFEQEVPVLKAKRTPVNCYTVNCSKCLFTCHSNCFLPEDDPLSTCAAMDEAGHCTVCSGLCNHSAHIKEKALWTYETKMEKQTIDELKENFMKAKGKFMNTKQMLDELESEFDVIEEKLRQLIKLSSSCLQRLNAIALKPTSLSTREYIEILIKTEKEEKKPGFEDRILGLEKIKQECIILDKIAKGEDLLESEQQVIAERKRRLKTVAMKAKKIKDVVRQWEKVTKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7E3</accession>
    <name>A0A3B4D7E3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RAB5B, member RAS oncogene family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RAB5B</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558527.1">
        <property type="nucleotide sequence ID" value="XM_017703038.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7E3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019051"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028634.2">
        <property type="protein sequence ID" value="ENSPNAP00000019051.1"/>
        <property type="gene ID" value="ENSPNAG00000025574.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430508"/>
    <dbReference type="CTD" id="5869"/>
    <dbReference type="GeneTree" id="ENSGT00940000166096"/>
    <dbReference type="OMA" id="QSTRACC"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000995">
        <property type="entry name" value="RAB5B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23587" checksum="3DAD6B5A17A68DE1" modified="2018-12-05" version="1">MSSRASGRANGSLPQTKICQFKLVLLGDMAVGKSSLVLRFVKGQFDEFQETTIGAAFLAQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVFDITKPETFERAKAWVKELQRQASPNIVIALAGNKADLADKRLVEYEEAQTYAEDTGLLFMETSAKTAMNVNELFLAIAKKMPKTDTQNPTHAARHRGVNLQDPDAQSARSCCGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D834</accession>
    <name>A0A3B4D834_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574868.2">
        <property type="nucleotide sequence ID" value="XM_017719379.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D834"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019675"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029673.2">
        <property type="protein sequence ID" value="ENSPNAP00000019675.1"/>
        <property type="gene ID" value="ENSPNAG00000026329.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440495"/>
    <dbReference type="GeneTree" id="ENSGT00940000167237"/>
    <dbReference type="OMA" id="AFACVTM"/>
    <dbReference type="OrthoDB" id="9858120at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056622">
        <property type="entry name" value="ARM_FBXO47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038946">
        <property type="entry name" value="FBXO47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34098">
        <property type="entry name" value="F-BOX ONLY PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34098:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24467">
        <property type="entry name" value="ARM_FBXO47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="39"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="444" mass="51387" checksum="17268687654F3240" modified="2018-12-05" version="1">MASESSRFTSTRSYRRFRSQRRPHSLRTAKTGTLRSSPQGFFEKLPAEVFDFILDYLSAVDASVFSMASKTISDCIMRHMSTSAWRNRRIWQWFDCTSPEAEGSALDHFRRLGLLFKRCTLLLPTKDRLRFSYSVFSQVPCLAMDQCAAFPFCRGFVYYGVFLTTLIAGWDELECHRVFDFICENTNLQRTIEAAVTCRPGACHRLELVVRLFCRGVLLNQWQRRRDSFFWLMCILRPWPIVSQARLLFILFGPLQSDGKIGWQRVRDHVVGQGGLWDLAKAIILLHDHEETKGWRPDNVLSIMDEMMVTPLPWHSENMARLLILCGKNICYNILASKAINGRLSEISRLLIFLILVSEKDGYCMKWAVKMTQQVCQVFSVASEKWSFIQSVENMFSEVTMELYELVMAGNHNGDMDPLQHLCCLLNASAHFHTEIVYMSLRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAV3</accession>
    <name>A0A3B4DAV3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nurim</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nuclear envelope membrane protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nuclear rim protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NRM</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nurim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539356.1">
        <property type="nucleotide sequence ID" value="XM_017683867.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DAV3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021472"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032819.2">
        <property type="protein sequence ID" value="ENSPNAP00000021472.2"/>
        <property type="gene ID" value="ENSPNAG00000028565.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412019"/>
    <dbReference type="CTD" id="11270"/>
    <dbReference type="GeneTree" id="ENSGT00390000008146"/>
    <dbReference type="OMA" id="WSIWFPL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033580">
        <property type="entry name" value="Nurim-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31040">
        <property type="entry name" value="NURIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31040:SF1">
        <property type="entry name" value="NURIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021472.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="280" mass="31185" checksum="B393F0921D3C04FD" modified="2024-11-27" version="2">MTMEWTAVRPALLCAAALINFVFVFGTGADFVRFVSFRAIYHNITGGAPLCRDAVPWSIALRDSEVLRALGVDLALLALFSVQHSLLAWAPVKKACQSALGVLNRAMYCSTTALALQVLMRWWQPVTSAPCLWSVRSAPWDIWFPLICFVVHFLCWATICSILLIFDYPELLGLKQVYYECLGLGDPLLLKSPRAQRLYAHLRHPVCVELLLVLWLLPTLPLDRCVLAAYLSLYLALAHSLDAQDCAYLSAQLHSKLQLFSAPQGGAAHINSNNNEQKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DC11</accession>
    <name>A0A3B4DC11_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:dkey-112e7.2</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DC11"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031986.2">
        <property type="protein sequence ID" value="ENSPNAP00000021015.2"/>
        <property type="gene ID" value="ENSPNAG00000027993.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278674"/>
    <dbReference type="OMA" id="FCACLGY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031742">
        <property type="entry name" value="DUF4730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36878">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36878:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15873">
        <property type="entry name" value="DUF4730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021015.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="79" mass="8481" checksum="AE1E7D64FACB5598" modified="2024-11-27" version="2">AQPDHVTCSSASSSMINMATTNNTRRLFAVFCLFLTTLVPTVEAFDGGDAVALLLGMTITVVGFCACLGWYARKRNGQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGV0</accession>
    <name>A0A3B4DGV0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyltransferase family 92 protein</fullName>
            <ecNumber evidence="8">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 92 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGV0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041352.2">
        <property type="protein sequence ID" value="ENSPNAP00000022668.2"/>
        <property type="gene ID" value="ENSPNAG00000036295.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064359"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008166">
        <property type="entry name" value="Glyco_transf_92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21461:SF52">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY 92 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01697">
        <property type="entry name" value="Glyco_transf_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022668.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="414" mass="47655" checksum="B8F16259AE71B249" modified="2024-11-27" version="2">TITKPFIVYPDRTGTHKEIVHQETSGPMIKVVNHKAYVISSYVEHRLSSKTIRTVAIVFRSEQVQYYCLLCCNGINISAAARLDIHSDHFEFDYGTADITCQVPTTCTTPAYVAITSKTHEWGRSVWNIQSFQPVGNQQPRTENFAYAFTVCISVMYDYMNVLGLVQAMEMFKLLGVQKVAIYKTSCYPDTQKVLDYYVRQGFVDIIPWTVSSYIKVARGWKKSDSPGELEFFGQIVALNDCVYRYMYESQYVALQDLDELILPLKEDNWTRLIPQLEKIYQHHAGFEFENNVFPISLSSNKKPENTPGFWKQFKGENILPHVYRIPNDPNVFNNFKVIVNPRLVFKATVHGVLDSASGTVRVDPKIARMYHIKRYPSKNVRANSVIRDTRLWDYVDKLIPAVSEVLRQALNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DIJ9</accession>
    <name>A0A3B4DIJ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DIJ9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024222"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010667.2">
        <property type="protein sequence ID" value="ENSPNAP00000024222.1"/>
        <property type="gene ID" value="ENSPNAG00000006459.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166318"/>
    <dbReference type="OMA" id="QDSMAFR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034651">
        <property type="term" value="P:cortisol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF73">
        <property type="entry name" value="FERREDOXIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="48"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="163" mass="17989" checksum="0E27513D4B66ABD7" modified="2018-12-05" version="1">GVSKPCVHSLSTLLDTPTLSVHLVDVKSETTAHLLLHSLCWSSSSCKVLLHFVNQSGMKTSVSAMEGESLLEVVINKNLQIDGFGACEGTLACSTCHLIFEDGVYEKMEPMADEEMDMLDLAYGLTKLSRLGCQVTVERWMDGMTVRVPQEVKDRREKPEAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKG5</accession>
    <name>A0A3B4DKG5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">CCA tRNA nucleotidyltransferase 1, mitochondrial</fullName>
            <ecNumber evidence="19">2.7.7.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">TRNT1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:14434"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:76236"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="19"/>
    <dbReference type="RefSeq" id="XP_017547409.1">
        <property type="nucleotide sequence ID" value="XM_017691920.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017547410.1">
        <property type="nucleotide sequence ID" value="XM_017691921.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017547411.1">
        <property type="nucleotide sequence ID" value="XM_017691922.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKG5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023419"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012679.2">
        <property type="protein sequence ID" value="ENSPNAP00000023419.1"/>
        <property type="gene ID" value="ENSPNAG00000000565.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424108"/>
    <dbReference type="CTD" id="51095"/>
    <dbReference type="GeneTree" id="ENSGT00390000009678"/>
    <dbReference type="OMA" id="NLCPKPM"/>
    <dbReference type="OrthoDB" id="445712at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001966">
        <property type="term" value="P:thigmotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007632">
        <property type="term" value="P:visual behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000023">
        <property type="entry name" value="CCA tRNA nucleotidyltransferase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050264">
        <property type="entry name" value="Bact_CCA-adding_enz_type3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46173">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46173:SF1">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="22">
        <location>
            <begin position="56"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="23">
        <location>
            <begin position="213"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="431" mass="49562" checksum="58D0A4FDD142D6D6" modified="2018-12-05" version="1">MWGRIFLSPKLLSRIHLTWSHRHLFTMQLKTKEFESLFTDGLKSLADIFEKHRFELRIAGGAVRDLLSGQRPEDVDFATTATPEEMKRMFQAAGIRMINNKGEKHGTITARVHNENFEVTTLRVDVQTDGRHAEVEFTTDWQKDAERRDLTINSMFLGLDGTVYDYFQGYEDLQNRKVRFVGSAAPRIQEDYLRILRYFRFYGRVASEPDQHDPDTLEAIQENASGLAAISGERIWVELKKMLVGNHAAHLLELVYELGLAQYIGLPAEGNVEEMKQVCQKAYGTSPKPVTVLAALFRSPDDVERLDLRLKISREEKTLGLFLVRRRRDLVKGQDEHGGLKPFTDFVIDSRELDAQSKVLELLKYQGEVKLLAELKRWSIPRFPVSGHDLRRLGITTGKEIGTTLQELRDIWKKSRYQLGKEELLSTISRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMH5</accession>
    <name>A0A3B4DMH5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017579999.1">
        <property type="nucleotide sequence ID" value="XM_017724510.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMH5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024663"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009556.2">
        <property type="protein sequence ID" value="ENSPNAP00000024663.1"/>
        <property type="gene ID" value="ENSPNAG00000009311.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443701"/>
    <dbReference type="CTD" id="84818"/>
    <dbReference type="GeneTree" id="ENSGT00940000168503"/>
    <dbReference type="OMA" id="ECTADES"/>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027841">
        <property type="entry name" value="IL-17_rcpt_C/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF12">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15037">
        <property type="entry name" value="IL17_R_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017436310" evidence="11">
        <location>
            <begin position="17"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Interleukin-17 receptor C/E N-terminal" evidence="13">
        <location>
            <begin position="237"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="SEFIR" evidence="12">
        <location>
            <begin position="431"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="661" mass="72750" checksum="0E2AB1FBEF9C1651" modified="2018-12-05" version="1">MGLGGFVLVLLAGALASSELLEKHQLRQTLTCSQGLDECTADESFRVEPLDSGPVEVTGLGVKAALCCRNNQKCKACLLISVRLRILENEEISGGQLGEDADEDKDDAAAVTVCYYSGPSLPRYKVISFRVTPAARKSREHELTVVEDKGVYLGSSVNVRVRSFNRSATFPLQRKVCPSAEVEECEKCRTPRVVAVTDEQRGVVNLTVADEDTGQLQLLQMCVKRGEQGLCLQSSSSVPLHAVTPCMCFEVWMEGSTCRSKLCPFSEKEEFRGNVLRNTSLSVVHSKTNEGRPALSWNLAAPCRLEAEVWPCRMDAGSGQGCGEVLGFRVKRNESSQWWENNATLWTSGSFVDIGSKDRFLHCVMFEVDGKIFGPLCQHDTHRQRWSVPVLAMLLFLSLVGVAVCFLRSRLRGCISDWERSHCSAEARGEVLLLHASGADVLEAGLVCQLGTELSELGFGVSLDLWSQAELSSLGPGPWLHSKLGLLQRRRGKALLVLSGSALDTAQACWDSWIGEKKGGPEGAKKDQTAWCKPTPCFSDVFGSALSCIFSAHLKGGTAERFVLVHFDSQKLINGGRAMPMLFQGLELYSLPSESRQLLAELCPEQPESFSMRIKKLLWLRRASRHLTKGLKNSGKRLWPQAQSVLTQVEILEEETLPLHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DML9</accession>
    <name>A0A3B4DML9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DML9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024705"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009451.2">
        <property type="protein sequence ID" value="ENSPNAP00000024705.2"/>
        <property type="gene ID" value="ENSPNAG00000009399.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048674"/>
    <dbReference type="OMA" id="CINPNNF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF278">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="7">
        <location>
            <begin position="13"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="452"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024705.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="506" mass="55276" checksum="4DFA3F54BA0F2163" modified="2024-11-27" version="2">MEIDEALLTLPINPYNFPARLWCLVNNPQICSIRWDSHGEAVIIDQQLFEAELLSPGRRALGKSPECFKTTNFTSFIRQLNLYGFRKLKPGDGILEKQIEGKPVTDNIVHNFHNPDFKQGHPELLVNLKRLTSINKAKLEAGLEVTCRPPSRFHRFLLNSPEGNITAEKRGSVLARQVHQRQISSSPYPCHSNASQQLKAYDRSSTPSSAWIMGHDSSSSSSAFYTDKDVPLYVLHHFPADMAYAVQSNPTSVHVPQGSQSSSAAGSKISTFIHPPPQYRSGFYSPVGQCCPPGSSDANMACSHQPAASYSHYGYYPVRTKYPLLFLYAYNIGQNLDWLSGDSMSKKTDVNLDTVFKIVDELQGSPKVMNMVKVSSPEKCPTTLTPENHLVGLPLHSSTACTSKPASAIASAQYDFPKVSRPIPVQSSPSFSSAPRGGIIITVPGNVSSGISITMGSHSSRSETGTDKGLELGSSQSEAAQLEPSLKVRKNHDDCHSLSATDAVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMV5</accession>
    <name>A0A3B4DMV5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">F-box DNA helicase 1</fullName>
            <ecNumber evidence="17">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">DNA 3'-5' helicase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">F-box only protein 18</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 26">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017554455.1">
        <property type="nucleotide sequence ID" value="XM_017698966.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMV5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024436"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010125.2">
        <property type="protein sequence ID" value="ENSPNAP00000024436.1"/>
        <property type="gene ID" value="ENSPNAG00000008968.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428173"/>
    <dbReference type="CTD" id="567995"/>
    <dbReference type="GeneTree" id="ENSGT00390000011669"/>
    <dbReference type="OMA" id="CERCVWT"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22095">
        <property type="entry name" value="F-box_FBXO18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18786">
        <property type="entry name" value="SF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000011">
        <property type="entry name" value="F-box DNA helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF30">
        <property type="entry name" value="F-BOX DNA HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="23">
        <location>
            <begin position="207"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1050" mass="119422" checksum="1C406D8FC0C42CA3" modified="2018-12-05" version="1">MESAVKGKAKRRHLSASECYGLSRSKAGSQSIAEPFSVNLHISDTYRGLQPRTPTKRRRRATEDGRHQRGITEYFASTGVLSSSPQKGKNEPTLKTEDEQNGHVVPKEEDEEQEVTKDYMEGITEDMFEDDEEFEMSGCSVKKEEDEEGRIAGCSSWGTPSVSTFNTEHSYVTVKKEEEEDEDYHVDSLPDIHYGLLGVNGGLDVPQGQFQDLPEEILSVIFAHLPADDLYRHVSLVCHRWREIITDPQFMPWKKLYYRYEKWEEEAVKEVKALLNENGIIKTDDLCVLNMVRYMSQFKHSRRVNMKDVSLSIKGHRLYAQAEACLKHRLPDMETIDGEPNPWSLMALMLILADGVKDILDLVARLRTSGCLLTPGGVSEYLWAVATLLLAMRDTNITISNRWHYNIFYVLHLIENASPPLSPGDGREKQFQVTHEQQQVLNHDIQRDHTVKIMAFAGTGKTTTLVKYAQQRSDLRFLYVSFNKSVATQANSSFPCNVECRTIHSMAYKAIGHRYRKLGKLCNNVQPFTVAWKLPEGRGGFVNAKVVTQTINNFCASTDTYIDTQHVPSVYRNTNGRDERPDEAKKRLFAGDAQDIWDKMVQMEPTREMAYHMTHDGYLKLWQMQRPVLKGYDVIFIDEAQDCTPVIMDIMLSQRCGKILVGDPHQQIYTFRGAVNALYTVPHTHLYYLTQSFRFGSEIAYIGATILDVCKKVKKNLVGGHQDGSVRGEDRETLESIKQATTQDRGKVAILCRCNVTVFSEAVRLTDANSSCKIHIVGGVENFGLRKILDIWILMQPEVKRMRDNLRIRDSFIRSFCKEKLGGYAGLKRYATHTEDRELEAKLSVVEKYNARIPELVERIYNCAQRSPDTADYILGTVHKSKGLEFDTVVVTDDFVKVPCSRHNLQRMNISPGIRNVDADEWNLLYVAVTRARRALYITKSICNILTLAGEYFLRSELSSTLLNKDVSLKCNTRECPNHITKDSPLSMCKVPVRHMDSVEEGGVLCATCVEQRVGPVAFLISPPEKVRAMPYTEERLELPINISMLLALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DNY0</accession>
    <name>A0A3B4DNY0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DNY0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024821"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009153.2">
        <property type="protein sequence ID" value="ENSPNAP00000024821.1"/>
        <property type="gene ID" value="ENSPNAG00000009590.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155128"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF10">
        <property type="entry name" value="FORMIN-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="33"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="666"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="828"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="836"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="862" mass="96886" checksum="B2ED66938CC25EA5" modified="2018-12-05" version="1">MTEPSPMIRAAIPPPPPPPPPPPLPPLLLNSRSSSSSSGSGRLRSLFWERIPQERVEGRRSVWSKSDEFSIDFSSLDELFRQHQGHSIKVLAVRGHCGGNKRTNMNEPPRNRLQRISFLKGNRSLKVGVFLHHFKSSLGGIINDIREGRGQRYGSERLKELYKLLPDCNEEQSLRSFGGERSQLEEADLFLLHLVEIPSFRLRLEAMILQEEFDPTVTSVSTSARCLATAATELMSCVELHSILRLVLKAGNYMNAGGFAGNAAGFRISSLLKLADTKSNRPSMNLLHFVAIEAVRKDPSILSYHLKLSHVGPAARLSVEAVEEEFSKLQSRVTALLVGLETAPDLLAQITPFLQAAELKLSKLQVDVDSVYKAQRSLQEFFCEDDDGSFKLEEACATFHNFQQRFLRATQENELREQQDQQRLEREQMVREKARKRRSIASCSALEAGLECDDNALEMILRAPAATPLRRKLWSTLGRRAPQHPTLISLIETPAENTSLPGNEAVIQAESPDIIAANKDADPMTKAELTGLSRLSAEVESVNRPAHSTCIQSKSWSLDGRLRPHVGETHTLVSVLRSYSSLAPPSGRADQKERKQKVRERSEEAEQEVTDVTSSQEDRKTRGKPREGTTKDVKPANQNQNSISTATPPFRASRLPLPRIGLWSTDQSGIASGTSENERLKAKIPQPLKRLLDRASNRKEKEKREKWKEPSEKQKTENDRDREKREKEKERKEKERERQGKERERKERQSGKKEQEKEREKREKEQVKSEKERQTTRMDGAKEMSVERSMRSGSALTAVVSRPLSRLCPAVAPSVLASVRCTAKVLHSALKRDRGSSSSRIPAPTSSRTLSLPRSDTRGQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DP19</accession>
    <name>A0A3B4DP19_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytoplasmic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017570399.1">
        <property type="nucleotide sequence ID" value="XM_017714910.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570400.1">
        <property type="nucleotide sequence ID" value="XM_017714911.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570402.1">
        <property type="nucleotide sequence ID" value="XM_017714913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DP19"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024689"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009471.2">
        <property type="protein sequence ID" value="ENSPNAP00000024689.1"/>
        <property type="gene ID" value="ENSPNAG00000009371.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437671"/>
    <dbReference type="CTD" id="445098"/>
    <dbReference type="GeneTree" id="ENSGT00940000157728"/>
    <dbReference type="OMA" id="HKMGKTE"/>
    <dbReference type="OrthoDB" id="26539at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903898">
        <property type="term" value="P:negative regulation of PERK-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902237">
        <property type="term" value="P:positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036493">
        <property type="term" value="P:positive regulation of translation in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10409">
        <property type="entry name" value="SH2_Nck2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11899">
        <property type="entry name" value="SH3_Nck2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11903">
        <property type="entry name" value="SH3_Nck2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11766">
        <property type="entry name" value="SH3_Nck_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000061">
        <property type="entry name" value="Cytoplasmic protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000110">
        <property type="entry name" value="Cytoplasmic protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000126">
        <property type="entry name" value="Cytoplasmic protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000027">
        <property type="entry name" value="Cytoplasmic protein nck1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017304">
        <property type="entry name" value="NCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035883">
        <property type="entry name" value="Nck2_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035559">
        <property type="entry name" value="Nck2_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035561">
        <property type="entry name" value="Nck2_SH3_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051184">
        <property type="entry name" value="Tyrosine-phos_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969:SF12">
        <property type="entry name" value="CYTOPLASMIC PROTEIN NCK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969">
        <property type="entry name" value="SH2-SH3 ADAPTOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037874">
        <property type="entry name" value="Cytoplasmic_NCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6 10" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="114"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="197"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="287"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="382" mass="42688" checksum="97E05EC3CD7ED12A" modified="2018-12-05" version="1">MSEEVIVIAKWDYTAQQDQELDIRKNERLWLLDDSKTWWRVRNASNRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTKRKTSARDTSPTPSTDAEYPSNGSGGGGAERIYDLNTPAVVKFAYVAERDDELTLVKGARLLVMEKCSDGWWRGSCDGQMGWFPSNYVQEEEAGEDGGDPPTSLASRPALVNGQAPRVLHTVQTLYPFSSATEEELNFEKGELMEVVEKPENDPEWWRCKNCRGQVGLVPKNYVVVLGDGPAAGGTGSHAQQYTGPAHTGRFAGKDWYYGDVTRHQAECALNERGVEGDFLVRDSESSPSDFSISLKAVGKNKHFKVQCSEGIYCIGQRRFNTMDELVEHYKKAPIFTSEHGEKLYLVKPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPA6</accession>
    <name>A0A3B4DPA6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PPIF</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPA6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008016.2">
        <property type="protein sequence ID" value="ENSPNAP00000025295.2"/>
        <property type="gene ID" value="ENSPNAG00000001389.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183116"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF102">
        <property type="entry name" value="CEREBELLIN 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C1q" evidence="4">
        <location>
            <begin position="49"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025295.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="186" mass="20231" checksum="5EA8B5D7DFF95D7E" modified="2024-11-27" version="2">FLTGCPPLRASVLTAPSVCSLVCMWCFTSRMVRRWNFPGCGIKKYHLDFIYLKVAFSASLSGGESGHTGPYSTDVTLVYKHIFTNIGHAYNPATGFFTAPVRGVYHFRFSLYGGGGRPATVVLHKNGHLIAGTHSVQPQGTVSSSNGVSLSLEVGDVICIKLNANAWIFDDSFHHSTFSGQMLFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPC3</accession>
    <name>A0A3B4DPC3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540961.1">
        <property type="nucleotide sequence ID" value="XM_017685472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPC3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025323"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007886.2">
        <property type="protein sequence ID" value="ENSPNAP00000025323.1"/>
        <property type="gene ID" value="ENSPNAG00000001391.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413118"/>
    <dbReference type="GeneTree" id="ENSGT00940000155258"/>
    <dbReference type="OrthoDB" id="9946452at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF366">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 47 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="148"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="524" mass="54925" checksum="0328C9956BE07F78" modified="2018-12-05" version="1">MGRSPPSPPSSESGGSVPPQKSYSSVRSIGSGSRWIGGGGARSSYGYSLGLGLGGGVHSQVGLGLGSGIGVGIGGGRLSASVGGSRLGASFGGRLGLGLASGGDAVGGGRLELGWAAGGGVGGVARGGAGFWTGGIADGGLHPFATNEKLELQSLNDRLAIYLDKVKKLEIANRELEEKLRGFKANKVPVTYDMQAYQIQLRPLQDQLADLLKDCSRLALLIDNAKLASDDYRIKYENEVAARQAVESDVAALKAMKIEYDLATAEMAQEYQILLKDRDTIQSTHEQEVLSLRGQVAGTLTVNVQAETSVDLSHRLADLRAEYENIAERNHREIENWYAGKMATKDQQISAVTEVTVTGSAEIVASRTQILTLQTELDAALLQKNQLEQHLVEVQGQNQNQLLTLSRLAGSLEGELASVRESALQQAREYQVLLSTKMQLEKEIATYKSLLEFAGDLSKFPGYSILSSWAQSSPAPSVDLRSLSPKASPAPSVSGEGAAAAGVVASSGSGSREGAAAAGPVICD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DR87</accession>
    <name>A0A3B4DR87_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">AP1S2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds to MUC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572111.1">
        <property type="nucleotide sequence ID" value="XM_017716622.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DR87"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026892"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003971.2">
        <property type="protein sequence ID" value="ENSPNAP00000026892.1"/>
        <property type="gene ID" value="ENSPNAG00000012260.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438658"/>
    <dbReference type="CTD" id="8905"/>
    <dbReference type="GeneTree" id="ENSGT00970000193372"/>
    <dbReference type="OMA" id="CNILEYR"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000009">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="14">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="157" mass="18578" checksum="8291805BBEF71FF8" modified="2018-12-05" version="1">MQFMLLFSRQGKLRLQKWYVPLSDTQKKKISREVIQMVLARKPKMCSFLEWRDLKIVYKRYASLYFCCAVEDQENELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYYILDEFILGGEAQETSKKIVLKAIEQADMLQEEAEAPRSVLEEIGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DR97</accession>
    <name>A0A3B4DR97_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger (CCCH type), RNA-binding motif and serine/arginine rich 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZRSR2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DR97"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026902"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003941.2">
        <property type="protein sequence ID" value="ENSPNAP00000026902.2"/>
        <property type="gene ID" value="ENSPNAG00000012282.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183152"/>
    <dbReference type="OMA" id="MDLRIME"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061484">
        <property type="term" value="P:hematopoietic stem cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061035">
        <property type="term" value="P:regulation of cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12540">
        <property type="entry name" value="RRM_U2AFBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000209">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="223"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="191"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="34"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="118"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="377"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="118"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="452"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="516"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="543"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="595"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026902.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="73613" checksum="79DD34C64A7B4D94" modified="2024-11-27" version="2">MNRPPVIDVQNFRAKLTEGYSSICLYTESEIATLTQPPQAAPPSGKQRRALLKRGRRKRKRQALAKLRLSVQSGIDQDHDDDYDCGDDKDEEERHRLHQEWLERERIAQEEFRLKQEKEEVARRRKEEEEKRIREEWEEQQRKEREEREQKQQEKRDREEAVQKMLDQAESQLENGGSWKNPDAPVDYGTEKDRANCPFFLKTGACRFGDRCSRKHDHPISSSTLMVRSMFVTFGMEQSKRDDYDTDASLEYSEEEVYQQFVDFYEDVLPEFRNAGKVVQFKVSCNFEPHLRGNVYVQYDTEDQCKEAFIMFNGRWYAGRQLQCEFSPVTRWKTAICGLFDRQKCPKGKHCNFLHVFRNPSNEFWEADRDLHLSPERGGGFTGWRSDRRDKASQRSYSPEQSHRRRSERRSRSRGRYRRRSRSRSRESRRSQYSLRRGQGSFSERYRRSRERCRSRSRDRGPHSPTDSRKRSSRRSESSSPGQEYKNGSVKRKSRKSMSKSPNGCDAAATDEITSSHRHKRSKKSKKKKKSKKKQKRKRSRSHSGSSSAGSGKETDEDEDEEPLQNQDKQSTEMPGDDRKVAVPLTALQEDNADHTSNSCQLEEENLPTTDVPTADAETDTLVGDDCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DS92</accession>
    <name>A0A3B4DS92_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">R-spondin Fu-CRD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RSPO3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the R-spondin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564618.2">
        <property type="nucleotide sequence ID" value="XM_017709129.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DS92"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026330"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005419.2">
        <property type="protein sequence ID" value="ENSPNAP00000026330.2"/>
        <property type="gene ID" value="ENSPNAG00000011609.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434172"/>
    <dbReference type="GeneTree" id="ENSGT00940000157815"/>
    <dbReference type="OMA" id="CMTSCPL"/>
    <dbReference type="OrthoDB" id="10257656at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051514">
        <property type="entry name" value="R-spondin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043601">
        <property type="entry name" value="Rspo_Fu-CRD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46987">
        <property type="entry name" value="NEUROHYPOPHYSIAL HORMONES, N-TERMINAL DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46987:SF1">
        <property type="entry name" value="R-SPONDIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15913">
        <property type="entry name" value="Furin-like_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="7 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043971846" description="R-spondin Fu-CRD domain-containing protein" evidence="11">
        <location>
            <begin position="22"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="R-spondin Fu-CRD" evidence="12">
        <location>
            <begin position="41"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="R-spondin Fu-CRD" evidence="12">
        <location>
            <begin position="143"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="246"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="250"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026330.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="316" mass="36079" checksum="7293A0388A795747" modified="2024-11-27" version="2">MQLQQLISLVLILHWMEDADCQHHASKHRQHKRGSPVCPGGCLTCSVYNGCLTCLPKLFIHLERDGMRQIGVCLASCPKGFFGTRSPEKNDCSKCGSECDTCFDKNFCTRCRAGSYLHKGKCQESCPDELVSSDTKRECVPPCPVDCDTCLNSETCTRCMPGYYLLHGQCHSVCPEEFEPSEQLMECVPAVHCEVGEWSEWGPCLRIGKPCFREETRTRKFLQNPSLQGKPCPATSEKRECIGKRKRCGKDKGAHKGDRKNRNNRKEKASGEGRRERKREREREREREMGDREDSENRNKTEQRRRRAQSRDPGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DT18</accession>
    <name>A0A3B4DT18_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Extracellular tyrosine-protein kinase PKDCC</fullName>
            <ecNumber evidence="3">2.7.10.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Protein kinase domain-containing protein, cytoplasmic</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Protein kinase-like protein SgK493</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Sugen kinase 493</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Vertebrate lonesome kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:10597"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017546122.1">
        <property type="nucleotide sequence ID" value="XM_017690633.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DT18"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027517"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002341.2">
        <property type="protein sequence ID" value="ENSPNAP00000027517.1"/>
        <property type="gene ID" value="ENSPNAG00000002522.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108422779"/>
    <dbReference type="CTD" id="559418"/>
    <dbReference type="GeneTree" id="ENSGT00390000001205"/>
    <dbReference type="OMA" id="HEGCLLS"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000552">
        <property type="entry name" value="extracellular tyrosine-protein kinase PKDCC isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042983">
        <property type="entry name" value="PKDCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448:SF3">
        <property type="entry name" value="EXTRACELLULAR TYROSINE-PROTEIN KINASE PKDCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="127"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="481" mass="54278" checksum="E29C7803B2727392" modified="2018-12-05" version="1">MKRRKVVVAAGFCLSFFLGTLFNLLSVPAFEHQQLGKQQQQQQQLEASLEPTTAAATSGHALLRSLRQQIRARLREALRYGPFPERGARPPLERRRLMDLQRAQEEEREEREHGSAPRLGCSSVDAASDVRFVGSGYTKAVYRAALNGSFAVALKSVDFGGHDMEECVRKYGSAGDCYRLASVKLVKEMALMERLQHPNILKLYGYCYQDSNDIKDTVTAITELGSPLEMIQLLQTPWEERFRICLSLMRLLHYLTHSPLGSLTLLDFRPRQFVLVDGELKVTDLDDASVEETPCSSSSDCLLEFPARNFTLQCSNGHCQGINEKRNLYNAYRFFFTYLLPHSAPTALRPLLDKIVNSTGELLWGTDETLAHLEKALDIYRSGQYLMNDTQMHKTEYKRVVDASIPGENYRCWPSYQHDGCMLSVYDVREAIGVCESHVQCRAFVLGNQSTWTGHQLVLFKTGSTSMTSAKGKLTYVKLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTQ5</accession>
    <name>A0A3B4DTQ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTQ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026364"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005312.2">
        <property type="protein sequence ID" value="ENSPNAP00000026364.2"/>
        <property type="gene ID" value="ENSPNAG00000011666.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017353"/>
    <dbReference type="OMA" id="RICNSAN"/>
    <dbReference type="OrthoDB" id="9944055at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042974">
        <property type="term" value="F:nuclear retinoic acid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026191">
        <property type="entry name" value="LRIF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16131">
        <property type="entry name" value="LIGAND-DEPENDENT NUCLEAR RECEPTOR-INTERACTING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16131:SF2">
        <property type="entry name" value="LIGAND-DEPENDENT NUCLEAR RECEPTOR-INTERACTING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15741">
        <property type="entry name" value="LRIF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="535"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="614"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="942"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="989"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="542"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="638"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="942"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="959"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="992"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1004"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1041"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1066"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026364.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1134" mass="122448" checksum="FC856867AD72E92B" modified="2024-11-27" version="2">MKIVSWNINGIRAFKNGIKKALDSFDADIICVQETKVTRDLLDERTAVVDGYNSYFSFSRGRSGYSGVATFCKDTATPFAAEEGLTGLLANLGDAVGCYGDHCAFSAEELQSLDSEGRAVITQHKFMRGEGSETLTVINVYCPRADPEKPERKLFKLQFYKLLQSRAETIEGLGSKTGVYYQAMPAVGPDGKNVMKLIPVQKVNGKFVQTQVLTTRNDCQPQVYSQPAHISPAYLPQNKLPTLQQTADGRFILKTSPESNILFNSVKIQHQGDNDSLKNAPNQVRAPLLTTRLTQNALPLLPPNNSKNVTVISGPQQPAALKSHVFPSGHYLQIPPNATVRTLPASALPQSIKSQICNSSSALSNHTKGLPTVVYVSPVNSLKLGPNQQVPGLTKASELPQSLAQTLSITDVKSLSVSSTCSPKVDQGASAPMKWIIQEGAGNTAPCLIPVSSPSMPTDILKAVRQIETVRHTNQVPAGEPTSADATQEKISPGMDNALVMCNGKVYFVAKKNSEISKDLISGVTGKVQVKNSVQPAGAASGSCTSDTVPKQDAKNTPVSKKPNEIIDLCDDDEVDEESSKRAESSRLTVLGETVGENDEDSNVIFVSYIPPKSDTETSKKVAETAPQNGSGTGMDSPECKEGNEEKSCETNGTDSLVIDHEVSRCQDNVAACESVSGAVSEQLQEVCDQGTAVTRKISVDPVIQCRQDSEAVTEMSLAEPAVENMPQRKSDSELRQIFGITSELKIGLRRINRAQESSPQVERRSINKRTLEGIRKLIRESQIELKIKQLIQAQVLVPKKEDDPEDAKRKRLEQVGQKTCLDSTCSIVQSSSPQTEDMVNCDDEMQSGSADACADSKVLLCCRGSESETLSTSGSSEVAKQTTELLCSFSCQPQEIQTACTHTDSTACTSVSRKTPHRQSKGSGRICTACPCGTKVGVITPNSSKPQNKTSSAAVVDPSEHASVFKESNRDCNTVDKVLENSVLDPPVINEELENSEDEAVLSGQSYSKPGESPLQSTASLAKQQDRASQAGLGSHGKTCRSSISRANTPGKTHEEGTGGHVASTKHDLYNRKEKAPRPSGGAMFGVGENMTSEFYSSIMLDPEEVKRRERIRRLKDLLKEKEAALEKLRKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUE4</accession>
    <name>A0A3B4DUE4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SH3 domain-binding glutamic acid-rich-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SH3BGRL</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Appears to function as an adapter protein that bridges proteins together or proteins with mRNAs. May function as a ubiquitin ligase-substrate adapter. Additionally, associates with translating cytoplasmic ribosomes and may promote the expression of specific mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer. Interacts with PFN1/Profilin-1. Interacts with ERBB2. Interacts with ATG12. Interacts with BECN1. Interacts with translating ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SH3BGR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559212.1">
        <property type="nucleotide sequence ID" value="XM_017703723.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUE4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026589"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004815.2">
        <property type="protein sequence ID" value="ENSPNAP00000026589.1"/>
        <property type="gene ID" value="ENSPNAG00000011852.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430904"/>
    <dbReference type="CTD" id="6451"/>
    <dbReference type="GeneTree" id="ENSGT00940000156017"/>
    <dbReference type="OMA" id="NEKEFMQ"/>
    <dbReference type="OrthoDB" id="9932926at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03030">
        <property type="entry name" value="GRX_SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000065">
        <property type="entry name" value="SH3 domain-binding glutamic acid-rich-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006993">
        <property type="entry name" value="Glut_rich_SH3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051033">
        <property type="entry name" value="SH3BGR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232:SF5">
        <property type="entry name" value="ADAPTER SH3BGRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04908">
        <property type="entry name" value="SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008142">
        <property type="entry name" value="SH3-bind_E-rich_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="115" mass="12909" checksum="29B96B5E929DAD61" modified="2018-12-05" version="1">MVIKVYIATSSGSTSIKKQQQDVMGFLAANKIEFEECDIAANEENRKWMRENVPEDSRPATGNPLPPQIFNEERYCGNYQAFFDAREDNAVYAFLGLTAPPGSKEAEAQAKKEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVN3</accession>
    <name>A0A3B4DVN3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Potassium inwardly rectifying channel subfamily J member 1a, tandem duplicate 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KCNJ1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DVN3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027533"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002299.2">
        <property type="protein sequence ID" value="ENSPNAP00000027533.1"/>
        <property type="gene ID" value="ENSPNAG00000013034.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282491"/>
    <dbReference type="OMA" id="LDLWTTI"/>
    <dbReference type="OrthoDB" id="273257at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000002">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003268">
        <property type="entry name" value="K_chnl_inward-rec_Kir1.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF6">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01321">
        <property type="entry name" value="KIR11CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="14">
        <location>
            <begin position="24"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="15">
        <location>
            <begin position="173"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="43936" checksum="8480B32C59A9247B" modified="2018-12-05" version="1">MTPTLSQRFRDYLEERRIHRNRLVTKDGHCNIEFGNVRYSNHFAYLLDLWTTILEFRWSVVLSLFTASFLFSWFIFGLIWFWIARDNGDLWWQNPPVDHNPCVISVVGLTTAFLFSFTSQMTIGYSIRVITPVCPGAIIVLVIQTLIATLVKSFWCGVVIAKMALPKKRAKTINFSKMAVICPRNDALCLQIRVANLRKSLLVGSQIYGKLLRTTVTPEGDTIIMDQISIDFMVDAGKDNLFFICPLTLYHEIDKTSPFFEMAVNTLQQQEFELVVFLDGTDEYTNFSCQVRTSYIPGEIMWGYEFLPIISHSKEGTYSVDFSEFSKVMPVTTPHCADCFNNEKAHYYDPRDGTDNPVFEASEIPNQEEQTSVNFRTVRKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1K0</accession>
    <name>A0A3B4E1K0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Fucosyltransferase</fullName>
            <ecNumber evidence="24">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = a beta-D-galactosyl-(1-&gt;4)-[alpha-L-fucosyl-(1-&gt;3)]-N-acetyl-beta-D-glucosaminyl derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14257"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:137941"/>
            <dbReference type="EC" id="2.4.1.152"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:14258"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a neolactoside nLc4Cer + GDP-beta-L-fucose = a neolactoside III(3)-alpha-Fuc-nLc4Cer + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48376"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90376"/>
            <dbReference type="ChEBI" id="CHEBI:90379"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:48377"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>alpha-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + GDP-beta-L-fucose = a neolactoside IV(3)-alpha-Gal,III(3)-alpha-Fuc-nLc4Cer + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48380"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90380"/>
            <dbReference type="ChEBI" id="CHEBI:90381"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:48381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>alpha-N-glycoloylneuraminosyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + GDP-beta-L-fucose = alpha-N-glycoloylneuraminosyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-[alpha-L-fucosyl-(1-&gt;3)]-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48388"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90383"/>
            <dbReference type="ChEBI" id="CHEBI:90384"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:48389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56076"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:136545"/>
            <dbReference type="ChEBI" id="CHEBI:139509"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:56077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>an alpha-L-Fuc-(1-&gt;2)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc derivative + GDP-beta-L-fucose = an alpha-L-Fuc-(1-&gt;2)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77191"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:133510"/>
            <dbReference type="ChEBI" id="CHEBI:195560"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:77192"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68044"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:145343"/>
            <dbReference type="ChEBI" id="CHEBI:176900"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:68045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-D-Glc + GDP-beta-L-fucose = beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-D-Glc + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:60239"/>
            <dbReference type="ChEBI" id="CHEBI:61352"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:77188"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="24">Golgi apparatus</location>
            <location evidence="24">Golgi stack membrane</location>
            <topology evidence="24">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Golgi apparatus</location>
            <location evidence="21">trans-Golgi network membrane</location>
            <topology evidence="21">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 24">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="24"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1K0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018619.2">
        <property type="protein sequence ID" value="ENSPNAP00000030497.2"/>
        <property type="gene ID" value="ENSPNAG00000017451.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164360"/>
    <dbReference type="OMA" id="YPETHIC"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017083">
        <property type="term" value="F:4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF10">
        <property type="entry name" value="4-GALACTOSYL-N-ACETYLGLUCOSAMINIDE 3-ALPHA-L-FUCOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 24" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 24" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="26">
        <location>
            <begin position="41"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="25">
        <location>
            <begin position="162"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="25">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="25">
        <location>
            <begin position="226"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030497.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="273" mass="32217" checksum="9C19D293354DC13F" modified="2024-11-27" version="2">GVPDHLQKWQFYLSRNCSSYPYIPYVNSDSSPTTPPTAQPEKPILLLWVWPENYRWDFSDCKTFYNIDGCHLTDDRSLYNQADAVLIYHRDIKWDLSNLPPSPRPPFQRWIWLHAESPTNNKRIPGLENLFNLTLSYRRNADISVRNLLTISKTPNKKFELPKKDKLVCWFVSNIDPSTGTGTRMKYYEELSEHIKVNVGWIACRFYLSFENSIHKDYITEERLPNASVLAQYLLQLDKDDEAYRRYFAWGKHVSATPHLVIVNQECRCFRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2Y8</accession>
    <name>A0A3B4E2Y8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">E3 ubiquitin-protein ligase RNF180</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">RING finger protein 180</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">RING-type E3 ubiquitin transferase RNF180</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 27">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with ZIC2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017573591.1">
        <property type="nucleotide sequence ID" value="XM_017718102.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2Y8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029599"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016871.2">
        <property type="protein sequence ID" value="ENSPNAP00000029599.2"/>
        <property type="gene ID" value="ENSPNAG00000015805.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439610"/>
    <dbReference type="CTD" id="793295"/>
    <dbReference type="GeneTree" id="ENSGT00950000182909"/>
    <dbReference type="OMA" id="CETQTQR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042415">
        <property type="term" value="P:norepinephrine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042428">
        <property type="term" value="P:serotonin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16554">
        <property type="entry name" value="RING-HC_RNF180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000316">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033263">
        <property type="entry name" value="RNF180"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045790">
        <property type="entry name" value="RNF180_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46717">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46717:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19332">
        <property type="entry name" value="RNF180_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="482"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="350"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00067421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029599.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="508" mass="58237" checksum="B55F8E666598C0D3" modified="2024-11-27" version="2">MASCLTDTVSDSVKTTEEPSILRCRKCRRCVADSACLLLASKEFTATCSVWHVDVETLPDWILKVINQVHWTVGKLNCQYCGARLGGFNFINCPKCPCGHNTAVHLSKSRVDQAVKHSVHLSRPGRARRHAEAQAGFLGKRKELEEPKVRGNLESIPNLNQDLHQVIQMLTSETELQSMPTQVTHLDGEASFLSPYSRTPDLPHLSSVDEDVTETVAIMDSGQEPQSIMSLETNNEGPEAHERQMAVESSGYQENTEGHLVYFTPQETPSLETELEQRQENIILGTPEEQMHHTTSSAVQKLTKKEKNRLKNIKKKQRKKAQWIQNQIEEKSVKWNLTSSDDEEKEGYTCAVCLDVYYNPYMCQPCSHVFCEPCLRTVAKNRPSSTPCPLCRTLISHVLFQQDLHQTTRTYFPKEYLSRKETFQKINYSKWPLPSCPKRFRILWGFQRRGAFTSRWQFPHRAFGLDGLDLGDMRGWPFDSDIVIVSIYSFHWVLAFILFCGLFYFFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3D0</accession>
    <name>A0A3B4E3D0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3D0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030260"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018143.2">
        <property type="protein sequence ID" value="ENSPNAP00000030260.2"/>
        <property type="gene ID" value="ENSPNAG00000035883.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165682"/>
    <dbReference type="OMA" id="WNVWEAA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000203">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF63">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY, POLYPEPTIDE F1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030260.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="525" mass="59300" checksum="36858BB66C942FC2" modified="2024-11-27" version="2">QHAVQRQRSSVKLWESLCDSGKVLVYPVDGSHWVNMKVLIEELHSRGHSITVIRPSSSWYITEHSPLYTSITVPDEIDSLGDFIEEHLNKMIEIQRGKISTLAFLKVQMNFFSMLSKAHLIACNTVSNIIDDNELVKRLHGEQYDLVLTDPAITTGVVLAHYLKLPLVLNVRWIISGEGHFIIAPSPLSYIPAPGSGLSDQMSFFQRVKNSLFCAIVLFQNTFVVGPHYKALCDKYFDEDCDIVSFLQEADIWLMRLDFVFDFPRPTMPNVVYMGGFQCKPAKPLPEDLEAFVQSSGEYGFIIMSLGTLVDSLPADTADEIAAVFAKLPQKVIWRHLGPKPSTLGNNTLLVNWMPQNDLLGHSKIKAFVAHGGTNGVQEAIYHGVPVLGIPLFFDQFDNLMRIQAKGAAKILELSQLDGQTFEQMLLEVLNDDSYRTNMQRLSSLHRDQPVKPLDSAVFWIEYVMRNKGAPHLRTKAYRMPWYSYHSVDVTLFLLSTAAVLTFTTVAFIRWVCCRACGKRKVKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E5D2</accession>
    <name>A0A3B4E5D2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5D2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019516.2">
        <property type="protein sequence ID" value="ENSPNAP00000030970.2"/>
        <property type="gene ID" value="ENSPNAG00000018041.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000205635"/>
    <dbReference type="OMA" id="ENCIEMT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="52"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030970.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="170" mass="19633" checksum="F16E44BFC96D8846" modified="2024-11-27" version="2">MVRLLKCVLCKGLVNFSQLTKRHLTRGTQTFKCNCTYHHFKNAECQDGWLQYGPRCFKHDHVIQYFQQNCVNMEGHLASVHSSEEYTFIQDLLNATNSNTTWLGGTDAAQEEVWVWTDGSAFNYPNWSPGQPDYLGFENCIEMTFPGIISFSQFLSFFYDKLISIMISVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7L1</accession>
    <name>A0A3B4E7L1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CHIC1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7L1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031760"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021011.2">
        <property type="protein sequence ID" value="ENSPNAP00000031760.2"/>
        <property type="gene ID" value="ENSPNAG00000019294.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000181562"/>
    <dbReference type="OMA" id="EVWIWIS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031760.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="172" mass="20006" checksum="B83A4169653E9A2A" modified="2024-11-27" version="2">NSSSCSCKRISVEFPSVENSELSPPAAFMFKLNVVVVVSEAPPFPMIFPASFTHVAPPPLMWVRRCLCNARLEEWVKLRPHSLQMYGRSPVCMRMCCRRLVDWLKALLHTTQTYGLTPRCTFLCLRRLLEFLKALGHLSHGYGHSPVCWRRWGQYGHWKVRKPVCTLRWTWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7M8</accession>
    <name>A0A3B4E7M8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho GTPase-activating protein 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rho-type GTPase-activating protein 29</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037386914.1">
        <property type="nucleotide sequence ID" value="XM_037531017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7M8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031683"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020870.2">
        <property type="protein sequence ID" value="ENSPNAP00000031683.2"/>
        <property type="gene ID" value="ENSPNAG00000019206.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415366"/>
    <dbReference type="CTD" id="378998"/>
    <dbReference type="GeneTree" id="ENSGT00950000183110"/>
    <dbReference type="OMA" id="DQYQSCM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20816">
        <property type="entry name" value="C1_GMIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04378">
        <property type="entry name" value="RhoGAP_GMIP_PARG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000016">
        <property type="entry name" value="Rho GTPase activating protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054713">
        <property type="entry name" value="GMIP/FCHO2-like_FCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057028">
        <property type="entry name" value="RHG29_45_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF7">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22699">
        <property type="entry name" value="GMIP-like_FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24235">
        <property type="entry name" value="RHG29_45_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="F-BAR" evidence="12">
        <location>
            <begin position="201"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="10">
        <location>
            <begin position="649"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="710"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="159"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="500"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="993"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1130"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1180"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1301"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="570"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="993"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1328"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1365"/>
            <end position="1374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031683.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1374" mass="149606" checksum="EA7E1E10CF838AF9" modified="2024-11-27" version="2">MFSQSGGNKLSSGAALSSAAWAMGRISSSNPPGAHPDYILQLVSDVRNFADVLLSLKEAFQSKEPVESLVGVVEDRLGELLRVLKDVIGKHQTLNSEEILSAAGTVIAKVKGVNFKEVNKENRSALFHEIHTSIDNLAFTFGNVVSDFLMGDVDSGSSVGPPQAGRSRSFENLSGDSAGCEEKDGLADSSVPVFPELSAVEKVDQALLRNDSGVESALLYAKAWSKYTKDVLAWVEKRLSLDVECAKSFAKLAESAKAVASQQDYMPFRDIYVSAFKNEIEYSQLFLQTAVALQTNKFVQPLVARKNDLDKRRKELKEQWQREQKKMTEADGAVRKARLLQAQRREEYEKARNSISRGGEEQQSAGGKGLEKKRRLEEEALQKAEEAQEHYKACVADAGAKRLNLENAKSEILTQIRELVFQCDLTLKAVTVNWFQLLHTQAASLPVKFQALCDQAKQYEPGQCYSEFVHSLPKERVWLEALAQDSTGPSRTGISLYHKRSLSSTHSSHGSGAGGDGLPADEQNSPAHRRVNKMANKRSSSTADIQGVKPQCPPQAWSSTGSVGGGGVGSDSESAGGSSETRSVDSPTASPGDFKRRLPRTPSTGTMSSADDLDEREPPSPSDNGLGEMIMEAASSPGPFRNAQMSKAAHTHKLRKLRAPSKCRECDSLVVFHGAECEECSLACHKKCLETLAIQCGHKKLQGRLHLFGIDFAQAAKNSPDGIPFIIKKCTSEIESRALNIKGIYRVNGAKSRVEKLCQAFENGKDLVELSDLYPHDISNVLKLYLRQLPEPLILYRYYNEFIGLAKEAQSVLVEEAESAQSRPQGEEPCLSIDLNRIIFKIRDLLRQLPPAHYRTLHFLIAHLHRVTERAEDNKMTASNLGIIFGPTLIKPRQMEAEVSLSSLVDYPYQALMVELFITHHNMIFDVPLSPVAPLTAPALHGSPSPHTRDKDRLSRHSRSLLDIKEQSSKAYKRHSSVIPSSHLLDEVKEVKLGDTKEGKEDTSQAADGTDGLNGVGSAAEVQRSTLVFGRPSTTSVTSMSSSLVQLRPQRSRFASRPVSMPVDRLLNERNRQNSAERESFPAVIQEVAEPEKSDGRYRSPFVHTHTLRRTWDKQYRHYDVTPRTAMIVANLPPPGVTDKTKTATGADPASGPSAVSMASQSSPFMVSNTVTIRPFRASWKDSSSTQSGEGPIAFRAPRTLQPPPGTFYKPPTALRARLSDGEKSATSSTTASVTTAMDLGTVTTSSTPASVTIATVTATSPVTTATVVTVVSMTMGAPVATLTVSTTRATAAATPAVATVPLSKPSEASEDSRSESGSAGSDAVSLSAFSPLQSPSSTPEDLSPTDSKPAYQRLRGSRRLIPNELERREAHFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8I1</accession>
    <name>A0A3B4E8I1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="5"/>
    <dbReference type="RefSeq" id="XP_037391596.1">
        <property type="nucleotide sequence ID" value="XM_037535699.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391597.1">
        <property type="nucleotide sequence ID" value="XM_037535700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8I1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032902"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023276.2">
        <property type="protein sequence ID" value="ENSPNAP00000032902.1"/>
        <property type="gene ID" value="ENSPNAG00000021158.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411635"/>
    <dbReference type="CTD" id="100002993"/>
    <dbReference type="GeneTree" id="ENSGT00940000166720"/>
    <dbReference type="OMA" id="HWLNMNI"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000021">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000203">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF120">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY, POLYPEPTIDE E1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017100839" description="UDP-glucuronosyltransferase" evidence="5">
        <location>
            <begin position="25"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="533" mass="60136" checksum="02AF9A002AD12063" modified="2018-12-05" version="1">MLFRCPVLLLLASLLWTQVPLSSTGRILVYPVDGSHWLNMNIVLKELHQRGHELTVVRSSTSWYIPEKTAHYTSLTIAITHASHLENPEFMASFLKRNIDIRRGEGSILSFIALQQETINLLEEAHRASAEMVRIILEDKRLVKKLRDTNYELFLTDPGFGGGVVLGAYLGLPIVLNVRWITNGEGHFAIAPSPLSYIPTIGSEVTDKMTFKNKLKNLLHYGIGQYIDHITTRPLYQGVISEFIDPDASIYSLIQGADLWLMRVDFVFEFPRPTMPNVVYMGGFQCKASKPLPADLEKFVQSSGEHGVVVMSLGTFLGSLGPEISEIIASAFARLPQKVVWRNLGPKPSTAGNNTLILDWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPVLGIPLMFDQFDNMVRLQARGVAEVLEVTALDVDTLTQALKDILDEKKPYRKNMRRMSQLHHDTPLKPMDTAIFWLEYVMRHKGAAHLRTESYKLPWYKYHNVDVFALVLGIVMVLGLLFSMTCRVLINLLKRKVKVKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EBP6</accession>
    <name>A0A3B4EBP6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Asparagine synthetase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017568995.2">
        <property type="nucleotide sequence ID" value="XM_017713506.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017568996.1">
        <property type="nucleotide sequence ID" value="XM_017713507.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EBP6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032656"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022773.2">
        <property type="protein sequence ID" value="ENSPNAP00000032656.2"/>
        <property type="gene ID" value="ENSPNAG00000020734.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436802"/>
    <dbReference type="GeneID" id="119261520"/>
    <dbReference type="GeneTree" id="ENSGT00390000012446"/>
    <dbReference type="OMA" id="SVYESCP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03766">
        <property type="entry name" value="Gn_AT_II_novel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="6">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032656.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="611" mass="67924" checksum="BD9664DEBA96A88D" modified="2024-11-27" version="2">MCGICCVVNLTASQCVLQKHVREKLRNRGPDCSQDITDMVLDPSYSCLFSAHVLHMRGVLTPQPLQDKDGNILLWNGEVFGGLTVGAEDNDTKVVLHHLSLCRSPSDVLSVLSQVKGPWAIIYYRKAEHCIWFGRDFFGRRSLLWSSGPEECSFTLTSVSSCLPDSDSAYWQEVPAVGVYSLDLKKCSYNRSLKLELYPWVYIGDEPPNDFTCEGLPSSVSMVMNDSGLLLNFPVPSMNTSLTAATPNDKPNQTSQTSPDDLKVFLKSTEKREMVRNLIDVLSEAVRRRVQCLPYQLKLTEEPNDDEAQVAILFSGGIDSMILAVLADRHIPADRPIDLLNVAFKLQESKINPGSTKKGQTNKRQHASADIAQSDQVKSNCFNVPDRITGRAGLQELKNLSPARKWNFVEINVTQEELKEMREKHISHLVHPLDTVLDDSIGCAVWFAARGTGIISEGTEQRRHTSTAKVVLTGIGADEQLAGYSRHRVRFKTSGLKGLVKELAMELGRISSRNLGRDDRIIGDHGKEARFPYLDEDVVSFLNSLPVWEKADLCLPRGIGEKLLLRLAAVELGLGPSAVLPKRAMQFGSRIAKLENRHEKASDKCKRLVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECP2</accession>
    <name>A0A3B4ECP2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease subunit Sen54 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TSEN54</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN54 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572483.1">
        <property type="nucleotide sequence ID" value="XM_017716994.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECP2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034312"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025956.2">
        <property type="protein sequence ID" value="ENSPNAP00000034312.2"/>
        <property type="gene ID" value="ENSPNAG00000003770.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438890"/>
    <dbReference type="CTD" id="283989"/>
    <dbReference type="GeneTree" id="ENSGT00390000004214"/>
    <dbReference type="OMA" id="AMVLQHI"/>
    <dbReference type="OrthoDB" id="408683at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024337">
        <property type="entry name" value="tRNA_splic_suSen54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024336">
        <property type="entry name" value="tRNA_splic_suSen54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12928">
        <property type="entry name" value="tRNA_int_end_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen54 N-terminal" evidence="4">
        <location>
            <begin position="64"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034312.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="527" mass="60945" checksum="CAA4995C83D59993" modified="2024-11-27" version="2">MAAKDTLTEAVQVEFSNELLSPPELLHARSRNHKIPVKGQKDFLLSGSEQQRAALQKSLDEHWTLVAEERVERLGNLVNAEWIPEEKLVKLQTPAGKFWQTMGFSDRGKQCLLPEEALYLMECGSVQVFYQDLPLSIQEGYEQFLSPETVTLHQYQVFGHLKRLGYVVNRFDPSTVPTLYERQLNLPLPRNKQTKHLKRKHGNSPLGSRLFNKSHCDKRSTLPVEETKPAQQCEGEIMTVDPRLDQTAPDQAKTEQNPQDELMEFNTDLDRAPRRNWWTDTSSQVQHTLGQHTSARPWDFSCIRFPDLGSSRAGHACLAAPDPKLLPGALQVGECEVTPWLRKLNLKAERLSRREWERQRERDRYHRDINNDREVRRCRNWAEYLELMEKRRSHQFKKRPAHLWEQDVIPLAQPGQCDSHRDLLEQVSVIQSSDLLHGASRLPPSEQWRICFNVYQPDTVADFKKSHPGKPYTRMCVCSFDGPVPDLLVMKQLSFQSGDVPVTFAVVDHGDISFYCFKEFKLPTDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFE5</accession>
    <name>A0A3B4EFE5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitogen-activated protein kinase 6</fullName>
            <ecNumber evidence="3">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MAPK6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017574989.1">
        <property type="nucleotide sequence ID" value="XM_017719500.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017574990.1">
        <property type="nucleotide sequence ID" value="XM_017719501.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037395941.1">
        <property type="nucleotide sequence ID" value="XM_037540044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFE5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033936"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038257.2">
        <property type="protein sequence ID" value="ENSPNAP00000033936.1"/>
        <property type="gene ID" value="ENSPNAG00000022915.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440574"/>
    <dbReference type="CTD" id="5597"/>
    <dbReference type="GeneTree" id="ENSGT00940000154351"/>
    <dbReference type="OMA" id="EADWQLH"/>
    <dbReference type="OrthoDB" id="8806754at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07854">
        <property type="entry name" value="STKc_MAPK4_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000136">
        <property type="entry name" value="mitogen-activated protein kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000223">
        <property type="entry name" value="Mitogen-activated protein kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008350">
        <property type="entry name" value="MAPK_ERK3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01771">
        <property type="entry name" value="ERK3ERK4MAPK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="20"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="722" mass="81579" checksum="26C6F02C34C491ED" modified="2018-12-05" version="1">MAEKFESLMNIHGFDLGPRYMDLKPLGYGGNGLVFSAVDTDCDKRVAVKKIILTDPQSVKHALREIKIIRRLDHDNIVKVFETLGPSGRRLTEDVTSLTEVNSVYIVQEYMETDLCKVLEQGPLSEGHARLFMYQLLRGLKYIHSANVLHRDLKPANLFVNTEDLVLKIGDFGLARIMDPHYSHKGHLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILESIPVIHEEDRQELQSVIPVFIKNDMSVPHTPLAKLLPGVSAEALDFLEKILTFNPMDRLTAEEALAHPYMSDYSFPLDEPVSSHPFHIEDEVDDILLMDESHSHVYNWDRYHDSQLSEPDWHLHSTVEAEEVQRDPRALSDVTDDEEVQVDPRKYADADSEKFLDDPAAFDFPPERSWPQYDDHHENKYCDLECSHTCNYKAVSPSYLDNLIWRESEVNHYYEPKLIIDLSNWKEQQSKEKGDKKSKSKCEKNGLVKAQIALQEASQSPPEKDRKQEKHQNQGFDFDSFIAGTIRLSLQPEPSQVGLLNELNTSVSQLSKSVSQEKDEKCQVNLAQQGGRPPCPWDSYGSPGPDESYCLIDEACWDAQKEGNVGGGGGYTSYLDRLFSKRDESAMITPPESPEDFSPDADIPLSSTQEETAPGFLARNGEIVLETLAIPGDLPLKSLQATLTPTTHVKCSPQIAHKTYSSILKHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFH8</accession>
    <name>A0A3B4EFH8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017542922.1">
        <property type="nucleotide sequence ID" value="XM_017687433.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037389475.1">
        <property type="nucleotide sequence ID" value="XM_037533578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFH8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034049"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025534.2">
        <property type="protein sequence ID" value="ENSPNAP00000034049.1"/>
        <property type="gene ID" value="ENSPNAG00000023089.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414553"/>
    <dbReference type="GeneTree" id="ENSGT01120000271828"/>
    <dbReference type="OMA" id="KLECVAW"/>
    <dbReference type="OrthoDB" id="8936120at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017435302" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="16"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="205"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="349" mass="40312" checksum="EBC3DE4528F303E5" modified="2018-12-05" version="1">MKLFLLLLCASHTAAEIYLYITKFTAVTGIAGLPEFTAVSFLHGKQIDYYDSKCHTLLPRQVWAKRVFGDPYWQRTTRLRFSESLHLQNGLSSAMESSGHKEGVHTFQRMYGCLWDEDTGYSDGFDVYGYDGVNFITLNLESRRFSALVPEAKSMAQEWNRNTTQIETLLLYYTHECVAWLKTFFVKNHKQHKYWGAAYQYYEFEAIVIPTITQHLRPGLLSVVVCHVTDLSTETVQVSWQKNEEDVKDYVEIGETLPSGDGTFQKTIYLAVRLEELKKNKYSCAVEFRTLTGKSLLTISQDTLFKMNYGAHSHTHARHSKWGSNDEDDYDDEAYVDEDYDDDDDDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ERG8</accession>
    <name>A0A3B4ERG8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NISCH</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017560294.1">
        <property type="nucleotide sequence ID" value="XM_017704805.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000037889"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034791.2">
        <property type="protein sequence ID" value="ENSPNAP00000037889.1"/>
        <property type="gene ID" value="ENSPNAG00000029982.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431562"/>
    <dbReference type="CTD" id="11188"/>
    <dbReference type="GeneTree" id="ENSGT00940000156494"/>
    <dbReference type="OMA" id="WSKNDLC"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06875">
        <property type="entry name" value="PX_IRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000020">
        <property type="entry name" value="nischarin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000468">
        <property type="entry name" value="nischarin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037904">
        <property type="entry name" value="Nischarin_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057714">
        <property type="entry name" value="PH_NISCH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF35">
        <property type="entry name" value="NISCHARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25625">
        <property type="entry name" value="PH_NISCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="7"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1417" mass="159680" checksum="7B84F2F477C5C568" modified="2018-12-05" version="1">MDCTAFHEQTVERNVCIVGSELVENYTVYIIEVKVGDHRWTIKHRYSDFHDLHEKLTSEKKIDKHLLPPKKIIGKNSKSLVEKRQKELEVYLQTLLGRFPVAAPKVLSIFLHFHFYEINGIAATLAEDLFHRGEQLLVAGEVFLLRPLQLYAITQQLKLAKPTCANGDAKADLGHILDFACRLKYLKIPGTKGVVGTSNIEEHSLPFDLSFFKSLLQIEITDCSSAQIKGLPSLKPTLATLSIHLSTASMKEILVPESAEFAQWEPEGVDIDCPVTAIIPTWKMLTTLDMSRNYICSIDESVKLIPEVEFLDLSHNELSLVENLQHLYNLVHLDLSFNKLTVLEGVHTKLGNIKTLNLAGNQLETLSGLSKLYSLVNLDLSSNRLAQLDEIKHIGTLPCLEKLSLANNPMCIIPDYRTKVLAQFWDRASEVCLDGTVTTEKELDTVEVLKAIQKAKEVKDRMANNEKKISDVPKHAEGGSKSSSSSCSLAAPLSSSPPLSHFASSSQEIACKDEAFVTKEILTHVDSTLTQRCCNKYNMSCEETPQVKTEIDTCSSPEIPCEPMKYESTSCLYCKDDQEPWTSKLSTPVLPFCYMSYTNTNLDFKAQLSTLICQADRERTAARQCRTTSLEVTHQGSPDSGGGYFEMGLDNSEEIMGSSSSSTVQDKESEDVERANVRLVMWCHCITVAQDVRLNQRTSCLAATDSLLVIFQTRSKDLPSSMQALMESLEIGLMVQYTDVEAFQFDIPETCLSLKVRSSDKQWFFFSDGQNLKDIHTHVDLLMSRAPGELLNESTIQQFIQQFLNSWEIEENDSGVKSGLLTHILDHASLFTPSQEDTNKPQQSSIPSDILSMLHEGHTSIPLVLFLTMRHLCILKIDFMALASKDLTEGNRMRSCAKLTRIPLASTLLNPKQGCTDGSNTIAHRFCNSHVLELMAGHDYLAVVFALPHDKFLFLRQFSQFRASLRDIKTIALLQTSKCHLRVSSATPSKTTQRMQKKSAPQDLQKPQLTLSLLYPGEALIQQLSDENQCPSHLCLSSSLRYLSSLRGEDLVTFFHDNIAEVENEELKHMLWTSVIFYKSPELEMTACILLSTKAVYFILDDAASSLSDQAMMWNWHPSEHKDTDFLISFCFAMKLNELQSVNVGLFDQYFRVVGPSAEHIITCLTRDSYSTHHFLQQLMTVLSLLEKVPSPEPSEQDFYTQFGKKSTGKMENYELVHSSKVKFVYPSEEEIGDLTFIVAERKGSQGSSHSFNILFYVLVFQVRSVSGHSAEKQHQLQPKTLILTSTDIFLLDEDYISYPLPEFAKEPPQRDKYCLTDARRIRDLDRVLMGYQTYPQALTLVFDDVPGPDLLCHLTMDHFADEQERGERMQGNSGAESEVQWCIFVPSAESRERLISLLARQWEALCSRELPVELTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IHQ2</accession>
    <name>A0AAR2IHQ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ankyrin repeat and IBR domain-containing protein 1</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017551906.1">
        <property type="nucleotide sequence ID" value="XM_017696417.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IHQ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063058.1">
        <property type="protein sequence ID" value="ENSPNAP00000038698.1"/>
        <property type="gene ID" value="ENSPNAG00000025893.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426720"/>
    <dbReference type="CTD" id="566524"/>
    <dbReference type="GeneTree" id="ENSGT00940000165758"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20361">
        <property type="entry name" value="Rcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16774">
        <property type="entry name" value="RING-HC_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000003">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000040">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000129">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047564">
        <property type="entry name" value="Rcat_RBR_ANKIB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047563">
        <property type="entry name" value="RING-HC_RBR_ANKIB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="330"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="334"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="791"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="970"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1048"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="302"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="809"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1070"/>
            <end position="1079"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1079" mass="121625" checksum="715F66AB73635AF6" modified="2024-11-27" version="1">MGNTATKFRKSLISGDEALACHLYENNPQFKEALDPNASYGEAYQHNTAMHYASRHAMSRLIRSFLFCKDGNPNKRNVHNETALHMLCMGPHILLGEAALQPRLSCPQQDEQRRAECLQMMLRWTGAKLDQGEYERANVNAADNKKSTCLHYAAAAGMKSCVELLVKSEADLFVENEEHQTPCDCAEKQHHKELALSLESQMVFSQDPGAQEIEAEYAALDRREPYEGLNLQDLRRLKDMLIVETADMLQAPLFTAEALLRAHDWDREKLLEAWMSNAENCCQRSGVQMPTPPPSGYNAWDTLPSPRTPRTTRSTLTSPDEISLSPAEEGLAPCGICMCSISVFEEPVDMPCGHEFCRACWEGFLNVKIQEGDAHNIFCPAYDCFQLVPVEVIESVVSREMDKRYLQFDIKAFVDNNPAIRWCPVARCERAVRLSRPGTAATDALSFPLLSAPAVDCGKSHLFCWECLGEAHEPCDCQTWKMWLQKVSEMKPEELAGVSEAYEDAANCLWLLTNSKPCANCKSPIQKNEGCNHMQCAKCKYDFCWICLEEWKKHSSSTGGYYRCTRYEVIQQVEEQSKEMTVEAEKKHKSFQELDRFMHYYTRYKNHEHSYQLEQRLLKTAKEKMEQLSKAFISREGTPPDTMFIEDGVQELLKTRRILKCSYPYGFFLEPKSTKKEIFELMQTDLEMVTEDLAQKVNRPYLRTPRHKIISAACLIQQKRREFLASVARGVAPNDSPEAPRRSFAGGTWDWEYLGFASPEMLRDHSVLGGREHSENFDSTGDQQQEYSDFQYRRRHRQRRRGDLLSLHSLRSNTPEPGDPNASTLDPQEGSGSRRYDVTTALGSLDEDDPNILLAIQLSLQESGLALDSEPQDVLSSDACLGAIGSSLPSRLEPVPHTVELPRASLSSSELLELGDSLMRLGNISSQYMPANLHSYTDGPGLDPTANANLLGNIMAWFHDMNPQNIALIPSANADASPDPPASDEALKLPPQPQEGDSGVEKPEQSLHFLVKENEDLERPSLLELECVDSSDLPKTDADLEELHYSVDLGSTAPHESDCDSHPQPTELSSEWEEQEHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IK66</accession>
    <name>A0AAR2IK66_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_037394388.1">
        <property type="nucleotide sequence ID" value="XM_037538491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IK66"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074131.1">
        <property type="protein sequence ID" value="ENSPNAP00000040110.1"/>
        <property type="gene ID" value="ENSPNAG00000034955.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413341"/>
    <dbReference type="CTD" id="334383"/>
    <dbReference type="GeneTree" id="ENSGT00940000156585"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF348">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC18-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="168"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="379"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="403"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="478"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="493" mass="53730" checksum="999E0411396AFCC9" modified="2024-11-27" version="1">MKNCEYQQIDPQALATPTPTPTPTPAPTPSARACPEKKPRRPQRKWEVFPGKNRFYCDGRIIVARQSGVLPLTLGLILVTSGLFFIFDCPFLVKHLTGFIPVIGGVLFVFVVISLLQTSFTDPGILPRATPEEAADIEKQIDNPTGSSSSYRPPPRTKEVVINQQVVKLKYCFTCKIFRPPRTSHCSLCDNCVERFDHHCPWVGNCVGKRNYRFFYTFIVSLSFLTAFIFGCVTTHLALRSQGGNGLVFALQESPASALELVVCFFSVWSILGLSGFHTYLLAANLTTNEDIKGSWSGKSGNEETGNPYSYNSIFKNCCSVLCGPMPPSLIDRRGFVPSEDGAEIGPAEIELPAAKNDVNMCTQGTKGLLETATRSPFLSSPCPQAKSQAIPPPALDALPSITPSPSPSPSPGPGPGPGPALEQTKVSRERPAATTPHRHHSHHRSRSKRTAQQGHNPGHASNPPASPGPRRSHSSRSRKDSKTRDPKFTPLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IW43</accession>
    <name>A0AAR2IW43_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 18">Nicotinamide phosphoribosyltransferase</fullName>
            <shortName evidence="18">NAmPRTase</shortName>
            <ecNumber evidence="15 18">2.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">NAMPT</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>beta-nicotinamide D-ribonucleotide + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16149"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.12"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:16151"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="14 18">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 18">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.12" evidence="15 18"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IW43"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042197.1">
        <property type="protein sequence ID" value="ENSPNAP00000042262.1"/>
        <property type="gene ID" value="ENSPNAG00000024413.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153456"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047280">
        <property type="term" value="F:nicotinamide phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01569">
        <property type="entry name" value="PBEF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000080">
        <property type="entry name" value="Nicotinamide phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041529">
        <property type="entry name" value="DUF5598"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016471">
        <property type="entry name" value="Nicotinamide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006629">
        <property type="entry name" value="PRK09198.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43816">
        <property type="entry name" value="NICOTINAMIDE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43816:SF3">
        <property type="entry name" value="NICOTINAMIDE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18127">
        <property type="entry name" value="NAMPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005943">
        <property type="entry name" value="NMPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 18" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5 18" id="KW-0202">Cytokine</keyword>
    <keyword evidence="4 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 18" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinamide phosphoribosyltransferase N-terminal" evidence="21">
        <location>
            <begin position="28"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="20">
        <location>
            <begin position="196"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>beta-nicotinamide D-ribonucleotide</name>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="319"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>beta-nicotinamide D-ribonucleotide</name>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="361"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>beta-nicotinamide D-ribonucleotide</name>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>beta-nicotinamide D-ribonucleotide</name>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>beta-nicotinamide D-ribonucleotide</name>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035007"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005943-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF18127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="500" mass="56702" checksum="9CC91958C205BE8C" modified="2024-11-27" version="1">MFCSCSCGLFKKRYVLFFRAAVPLGSAVTHYKQYPPNTSKVYSYFECRERKTDPTKTRKVKYEKTVFYGLQYILHKYLKGKVVTPEKIQEAKEVYREHFQDDVFNEKGWNYILEKYNGHLPIEIKAVPEGSVIPRGNVLFTVESTDQECYWLTNWVETILVQIWYPITVATNSREQKKILAKYLMQTSGNLEGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGIGVIKKYYGTKDPVPGFSVPAAEHSTITAWGKDHEKDAFEHIIKQFPSVPVSIVSDSYDIYNACEKIWGEDLRTLVEKRSADAPLVVRPDSGNPLDTVLKVLEILGKKFPLIENSKGYKVLPPYIRVIQGDGVDINTLQEIVEGMKEHRWSIENIAFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVADPNKRSKKGRLSLHRTQSGDYVTLEEGKGELEEYGVDLLHTVFLNGKIVKTYTFDEVRDNARLKDSEVEELLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWT4</accession>
    <name>A0AAR2IWT4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWT4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057901.1">
        <property type="protein sequence ID" value="ENSPNAP00000044155.1"/>
        <property type="gene ID" value="ENSPNAG00000031568.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163911"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
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    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="33"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="150" mass="17194" checksum="29F815B283B608A2" modified="2024-11-27" version="1">CPRDSFVPFFLSVYMVCKMIILEIQGFPLARHYYFVSESKSWTEAQTYCRKRFTDLATVDSVEDMSSLMKAVVAGYSGYWIGLTQAGQQHWGWSTGDEILSNFSNWDAGRPNGNGWCAFTVDGVWRDYDCGVALHFVCYYGKSTIFFFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYL0</accession>
    <name>A0AAR2IYL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084830.1">
        <property type="protein sequence ID" value="ENSPNAP00000043124.1"/>
        <property type="gene ID" value="ENSPNAG00000033066.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165119"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000020">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050969">
        <property type="entry name" value="Dev_Signal_Modulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949">
        <property type="entry name" value="EGF-LIKE-DOMAIN, MULTIPLE 7, 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949:SF54">
        <property type="entry name" value="VWFD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="110"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="242"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="264"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="339" mass="36114" checksum="CD5FF88A173F5B70" modified="2024-11-27" version="1">MCTNTPGSFQCSCQPGYQLHIDRYSCVDIDECKLQNGGCSHGCTNTPGGHICHCPAPLLLDHLNTICVNITSCSLRNGGCDHVCSLGAEGRVQCSCRDGWELANDQRICLAMCTPPCSHGGTCMRWNTCLCPPGWTGEGCHTAVCELPCGNGGRCVAPNTCHCPSDYAGPQCLTPLCTPPCLNGGRCVDVNTCSCAGAWQGARCQIEPVQCVKPCKNGGVCVGFNRCRCISGFTGTLCETAITTPCVPPCQHGATCSPHNTCTCPKGTSGLRCEKLTCPVVTTVVSMARAVRKGFRESYVDRCGPLGVQLCTKYRINQARVYLQAYRVGYKIQCPDKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J4W6</accession>
    <name>A0AAR2J4W6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J4W6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072973.1">
        <property type="protein sequence ID" value="ENSPNAP00000045392.1"/>
        <property type="gene ID" value="ENSPNAG00000033516.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="74" mass="7838" checksum="19DDD03B717672E5" modified="2024-11-27" version="1">GCITVCKCLSGCIIVCCISVCMGLLVSVRLYYCLSGCISVCMAVSVLVSVSVCMAVLVSVWLDCMTVLVSVRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDY1</accession>
    <name>A0AAR2JDY1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JDY1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055975.1">
        <property type="protein sequence ID" value="ENSPNAP00000048537.1"/>
        <property type="gene ID" value="ENSPNAG00000030021.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156131"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF6">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="11">
        <location>
            <begin position="348"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="174"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="265"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="722"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="790"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="859"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="195"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="723"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="795"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="821"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="859"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="939" mass="100886" checksum="DCFDB76FACE2CB1D" modified="2024-11-27" version="1">CYLLSPLQEELVVQQPPGVQGGPLSSSHAAPSSRYCTVDSPSRVFDCPSIQITSIPASCHQDTMVGLQDGVHHVEQQRASGPLASRDQLYLSLDPSYRDTAALCPSPCSSLSSRSWLSDASSCESFSHIYDDVEAELNEAAARVRLGSPLMSPQGSPLPSPLTSPQQLLYTCSLSPHQSPYQSPRASITEETWLSPRPHSSSSSRPSSRPTSPYGKRPYSSSEAVPGSSSPHLSPCPSPSHSPRGSVTEETWLVSPAAMAASFQASSSEIDIPTKTRKTFQSQEPTSLLPVHGDPGLKDQSFISPSLDSNLSLPTLKKEDIVEHFLSVPSAFAWGKPKQTQTPLFRPTSLPPLDSPLPSQYGQCELKLEIQPKPHHRAHYETEGSRGAIKSACGRHPIVKLIGYSEKPVSLQMFIGTADDRHARPHAFYQVHRITGKTVATPSQEVLIGSTKILEIPLLPENNMSASIDCAGILKLRNSDIELRKGETDIGRKNTRVRAVFRVHIPQATGTVLSLQVASIPIECSQRSAQELPQIERFSPPSGSVTGGQEMLIIGPNITPESKVIFLEKGPDGKTQWEVDAKIVCEKCTDTSIVVKIPPYHKRILTSSTQVQFYISNGKRKRSPAQRFHYISGKDPLKSIASAVLSFKAVQVKQECNVGDDVEDHGNPLDQWLYSGGPVCVPSSTQLSYSPMGSSYLDQHPLDGSFSTGHGLHSIFNPPSLDFSKQSYQGPPQNLSYNGQPSLPSDLASGGKLSPLVSEHAASLTGIGHHGSAGGVHPVNQISAPRYNEGYLSPSLGSNQPDLSCPNHYTSSPGHADLAGLPKSSSSLESPISHPPLASTVVPSTSPLNREALINLKSAKTLSPQADGTSGTETSLGPGASEDDSLDGKMLNIKQEPAEEKELSFQSIGLQDITLDDGKSLHDPCFLVVDVVVTGRLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JE80</accession>
    <name>A0AAR2JE80_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MAPK regulated corepressor interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MCRIP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCRIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567461.1">
        <property type="nucleotide sequence ID" value="XM_017711972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037400480.1">
        <property type="nucleotide sequence ID" value="XM_037544583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JE80"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067948.1">
        <property type="protein sequence ID" value="ENSPNAP00000048514.1"/>
        <property type="gene ID" value="ENSPNAG00000032309.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108435858"/>
    <dbReference type="CTD" id="348262"/>
    <dbReference type="GeneTree" id="ENSGT00940000161850"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029428">
        <property type="entry name" value="MCRIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14799">
        <property type="entry name" value="FAM195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10832" checksum="5E2285FF42EDD376" modified="2024-11-27" version="1">MTSSSTPRMHTYKRTSSPRSPTNTGELFTSAHEENVRFIHDTWQCVLRDIKSPQNSERGPQEYVEKNPNPNLHSFTPVDLSDLKKRNTQDSKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JJL6</accession>
    <name>A0AAR2JJL6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Syndetin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VPS50</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JJL6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081242.1">
        <property type="protein sequence ID" value="ENSPNAP00000050031.1"/>
        <property type="gene ID" value="ENSPNAG00000006716.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003442"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019514">
        <property type="entry name" value="Syndetin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040047">
        <property type="entry name" value="VPS50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019515">
        <property type="entry name" value="VPS54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258:SF0">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10474">
        <property type="entry name" value="Syndetin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10475">
        <property type="entry name" value="Vps54_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 N-terminal" evidence="7">
        <location>
            <begin position="54"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 N-terminal" evidence="7">
        <location>
            <begin position="213"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Syndetin C-terminal" evidence="6">
        <location>
            <begin position="756"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="392"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="997" mass="114672" checksum="9FBF34142CCA0130" modified="2024-11-27" version="1">MQKIKSLMSRQGLKSPQESMADMSPVENFRIPSKEELRELREQPIDPQAEQEIIDSIEEVYFSSDSFDMVQYELEKLPPDLNLQELEEYRDKLKRQQAAVSKKVADLILEKQPSYVKELERVTSLQTNLQLAAVMCTNARRQLSFAKEGFTEASLGLLANQRRRQLLTGLLKSLRTIKTLVITKLMTECGQLVSPWVPLSSVKSALFLSLMQQRTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKTCKHFDVSHYTKVQLAYSLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNADTKFQKMQYKDLCTHITPDSYIPCLTDLCKALWEVMLSYYRTMQWHEEHDKEEAADPDTDGTSNESPGDSTVGRSYVKKKLEHGLSRIWQDVQLKVKAYLLGTDMSNFKYDDFIVVLDVISRLMQVGEEFCGSKSEVLQESIKRQSVNYFKNYHRTRLEELRMFLENETWELCPVKSNFSIAQLHEFKFMGQCRSPSVSPSRQPASSEPVELSLFEQYTQVGNPFEMQIDNKEEETEDVLASNGYESDELEKSVYQDYDSDSDVAEELKQDFVDEQTGDIPAKSVSRETLRSRKRSDYNLNKANAPILTNTTLNVIRLVGKYMQMMNILKPIAFDVIHCVSQLFDYYLYAVYTFFGRNDMYESSGLGLISSRLRTTLNRIQESLIDMEAGGESVGPHGPPEERKEKVPSPHLSQLVVLTASGTLYGLAERVVATESLVFLAEQFELLQPHLDTMMPPAKKPFLQQFYSQTVSTASELRKPTYWIVAAKAIDYEQMLLLMAGVKWDIKEIMSQHNVYVDVLLKEFEQFNKRLGDVSRQVRIPLPVSNVLWEHCIRLANRTLVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDLRPIPDKEFVETYIKAYYLTENDMEQFIKNHREYSMKQLANLVNVCLGSHINKKARQKLLAAIDDIDRPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JN82</accession>
    <name>A0AAR2JN82_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JN82"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051097.1">
        <property type="protein sequence ID" value="ENSPNAP00000053470.1"/>
        <property type="gene ID" value="ENSPNAG00000036021.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278956"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="82" mass="8961" checksum="4DB116496838EBB8" modified="2024-11-27" version="1">MGVGIRRLGYGVGIRRLGYGVGIRRLGYGVGIRRLGYGIGIRRLGYGVGIRRLGYGVGIRRLGYGVGIRRLGYIKSSAIEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JND6</accession>
    <name>A0AAR2JND6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ITPR-interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JND6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043046.1">
        <property type="protein sequence ID" value="ENSPNAP00000051371.1"/>
        <property type="gene ID" value="ENSPNAG00000037594.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161762"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029325">
        <property type="entry name" value="ITPR-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043444">
        <property type="entry name" value="TESPA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469:SF14">
        <property type="entry name" value="PROTEIN ITPRID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469">
        <property type="entry name" value="SPERM SPECIFIC ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14722">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01257">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ITPR-interacting" evidence="1">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="91" mass="10454" checksum="E37BBA3D8284C035" modified="2024-11-27" version="1">EKGGIGKMIYGCDEYVMDVLNLWQDDPEELLLDLGFGAEEPDITVRIPARFINHQSKARGINIQLFLEAQKNRMDIENPDVSSEQPLNFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JPI9</accession>
    <name>A0AAR2JPI9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JPI9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066381.1">
        <property type="protein sequence ID" value="ENSPNAP00000052207.1"/>
        <property type="gene ID" value="ENSPNAG00000011487.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286911"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000538">
        <property type="entry name" value="Si:ch211-127b6.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000100">
        <property type="entry name" value="Si:dkey-11n14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000037">
        <property type="entry name" value="Si:dkey-191j3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="9">
        <location>
            <begin position="221"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="1077"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1265" mass="140594" checksum="9D0B289B4EA094C5" modified="2024-11-27" version="1">MNQNQRAEELTTKLKDGLHRSEEVTGQNTAASSTAAQTEDSVSGLTADQTGQVQTTGPQNEPVQTSTAQTGGTVNAPLLHGSVFNGPVNINFGADAKHRGGDRGEEETGKTSAASSTAAQNGVSVSALTAAAKEDGAVKDALKRHFVAKLAKLYDRTSLEGDNLLLKDIYTELYVIEGCTGGVNTEHEVRRIESFYPKIEEKPIKLSDIFKTRSSKNEFGTKVLTLGIAGVGKTASVHKFILDWAEDKCNQDLDFILFFPFRELNVIKEERYSLWNLLIYFHDEFHNVDMREILNHKCKVVFILDGLDESKLILHFNQKKLSKVTEVTTTDKLITNLIKGALLPSALIWITTRPAAAKKIPLEYIHHVTEIRGFNDPQKEEYFRKRIRDEDQASRIISHIKTARSLHILCHIPVFCSISATVLQEMLKDDKDMKNAPTTLTEMYTRFLLFQTRKKTEKYSTIQQEDGTVPPSERDRLGVEGVLKLGKLAFLQLQKGQLIFYEKDLKEGGIDVDEAVVYSGVCTQIFKKDEKIFSFVHLSFQEFLAAAFVFLTFRDKGNPLLQTIMEKIKWMFKHTLGDLLKTAVDKAMKSENGHLDLFLRFLCGLSLESNQRLLKSLQPEMDIKEESLKDTADYIKRKIKKKKHSEKSINLFHCLSELNDNSLTTEIQEYLNSGDLSTQNLSSTQWSALVFMLLMSEETQEKFELKKYRPSDEGLRNLLPVVKNTRRALFAGCNLTVQSSKMLSAALQTENSSLTELDLSNNDLQDSGVELLSAGLKSSDCKLEILRLASCNLGITACESLESVLTLETSSLKELDISSNDLPDSGVEVLSAGLKSSHCQLEILRLASCNLGITACESLESVLTLETSSLKELDISSNDLPDSGVEVLSAGLKSSHCQLEILRLASCNLGIKACESLESVLTLETSSLKELDISNNDLQDSGVEVLFAGLKSSHCQLEILRLASCNLGIKACESLESVLTPETTSLKELDISHNDLQDSGVEVLSAGLKSSHCQLEILRLSGCMITEKGCSSLASALSSNPSHLKELDLAYNHPGQSGVKLLSARLDDLHCSLNTLRLEHAGEIRIQPGPRKYSCEFTLDPNTAHRELTLSDGNRKVERVGFHSYPDHPERFDWCCQVLCRESLTGRCYWEAEWSGTVYIAVTYKSIRRKGDREDCEFGWSEKSWSLFCSDDSYSVRHNNNETKLSARPSSERVGVYVDCPAGSLSFYSVSDDQTLTHLHTFSTTFTEPLCAGFTVGSDSSVCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JVG3</accession>
    <name>A0AAR2JVG3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KIRREL3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JVG3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053763.1">
        <property type="protein sequence ID" value="ENSPNAP00000056043.1"/>
        <property type="gene ID" value="ENSPNAG00000008563.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157126"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05759">
        <property type="entry name" value="IgI_2_KIRREL3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05898">
        <property type="entry name" value="IgI_5_KIRREL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000094">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000103">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000170">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF49">
        <property type="entry name" value="KIN OF IRRE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043378067" description="Ig-like domain-containing protein" evidence="14">
        <location>
            <begin position="18"/>
            <end position="773"/>
        </location>
    </feature>
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        <location>
            <begin position="492"/>
            <end position="518"/>
        </location>
    </feature>
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        <location>
            <begin position="3"/>
            <end position="112"/>
        </location>
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            <begin position="120"/>
            <end position="213"/>
        </location>
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            <begin position="220"/>
            <end position="300"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="15">
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            <begin position="305"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="390"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="704"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="738"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="773" mass="84240" checksum="39A449288C4CAF4E" modified="2024-11-27" version="1">MLPGVFFLSWLVGFGLGAMLVQQPHDVVVVAGQPVTLPCAITGYHGMVLWVKDGLALGVNRDLSGYPRYDVIGDHSKGEYHLLIQRTELADDGSFECQGIQAAIRSRPARLTVLVPPDDPVIIGAPVVSLRAGDPLNLTCHADNAKPAASIIWIRNGEVLNGAMYSKTLLRDGQRESTVSTLYISPSNIESGQRITCRASNKAAPNGKEATVTIDIQHLPLVNLSVEPQPILEGNLVKFHCSAKANPPVTHYRWAKGSSMIRDVLGDSYEVVVDHSFFTEPVSCEVTNALGSTNISRNVDVYFGPRLAAEPQSLQVDLGSDAVFNCAWTGNPSLTIVWMKRGHGVVLSNENTLTLKAVKQEDAGKYVCRAVVPRVGVGEKEVTLTVNGPPTISSTQTQQALYGEKGQIKCFIRSTPPPDRIAWSWKETVLESGTSGRYTVETVSTEEGVISTLTMSNIVPADFQTIYNCTAWNSFGSDTEIIRLKEQGSLRLAVIIGVAVGAFLALIVLMGTLGAFCCARLQRKDMHLVMSSLPVSLTARQRELSSTIKAENLKGVVSAKNDIRVEIVHKDHNAARETEEHTNMKQMMMERGEFQQESVLKQLEVLQEEEKEFQHIKDPTNGYYSVNTFKEHSTPATSQATEVRNSATATLGKQRVPTGMSFTNIYSTLGPGPNHRLYDYGGRFVLGMGSSSIELCERELQRASLSDSSSFQDTQCDSSVSSYSRPDNGYAPFDKASKASASASASSSSHHSQSSSQNSDLTRPLQKRMQTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JW22</accession>
    <name>A0AAR2JW22_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JW22"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068927.1">
        <property type="protein sequence ID" value="ENSPNAP00000054404.1"/>
        <property type="gene ID" value="ENSPNAG00000033971.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248878"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="32376" checksum="9E9AE8A2FD02BC2B" modified="2024-11-27" version="1">SQTPGQLWQDNNYEGQSWIQNHQINLWHQESPIESQRQATPSVWQQESAPVSTKETLKLQRLRNVSVCEQKCACKNFYLCIFFFPIQFVECSNNVSSNGVSALLHDLLIKQEIIIEQQRNLIRTVQDLKTTSARENTAACEDTIACDMNQRHLPIEDLPSLMALENDLRSSPDKRKKLYLLINLGLIGGADIKDTVWRILKQTIKNDLAETVNWRGVNGKIGFQGLQLKNVVTEAVRRNTVCSQATEMEVERVIRRWFHLACDREGGRKRRKVENASGQNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JZF9</accession>
    <name>A0AAR2JZF9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GPI ethanolamine phosphate transferase 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PIGG</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGG subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JZF9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067887.1">
        <property type="protein sequence ID" value="ENSPNAP00000057375.1"/>
        <property type="gene ID" value="ENSPNAG00000024476.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144269"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051267">
        <property type="term" value="F:CP2 mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16024">
        <property type="entry name" value="GPI_EPT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000018">
        <property type="entry name" value="Putative GPI ethanolamine phosphate transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037674">
        <property type="entry name" value="PIG-G_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039527">
        <property type="entry name" value="PIGG/GPI7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045687">
        <property type="entry name" value="PIGG/GPI7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19316">
        <property type="entry name" value="PIGO_PIGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="464"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="501"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="684"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="713"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="749"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="787"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="875"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="917"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="948"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 2 C-terminal" evidence="12">
        <location>
            <begin position="641"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="977" mass="108093" checksum="62DF0BD05D5F107C" modified="2024-11-27" version="1">MRLSSSVFASFCLLIELLGIALFLRGFFPVPVKSSLSSKSKLSDLPAEPLAGNSPNSTKVPPPLFKRVVIILIDALREDFIFGPNGKRFMPYTRHIVERGSSHSFIAKARPPTVTMPRIKALTTGSIPGFIDVVMNLNSPAVLEDNVIWQAKNSGKRIIFYGDDTWVRLFPKHFMEYDGTTSFFVSDYTEVDNNVTRHLDSTLKRDDWDVLILHYLGLDHIGHISGPHSSLIGPKLLEMDDVIKKIHASLISREAEGTLPNLMVLCSDHGMSETGSHGGSSEPEISTPLILISPLFQRKVGLEQPAMVEQVDLAPTLALALALPISQNSVGRLIPAVFEEASLREQLRFLHMNGHQLSSLLRDSNPNFHKEDGYEQFRMAEKTHGNWMKLYMDGNTSEVLGNMGKKLLKQYLEALQAMSAALSKQLGKYDMYSMIVGMSLILQVLVLLVLAMPEALSRAACVDVPVASAFLSLPFYLLCLLGSSIHVLVCTSAEASCYFCSLPWLLAFTAILFSSALLCSLVAMAARYGEELVQCSTTRTSSDWTLSELDILLLTGTVGHTLSLGASSFVEEEHQTWYFLLNTLCLAVFQDVCRKYFRERRRPGDDEEDDPLLPSIAEEDASQATFSPAAELGVSVGSEKWLALSTPILTLACCRLLRSLNQTGVQWAHLPDLSHWLNSGDHKVVLSLLAALSLLLIFLLVQRRCSLVSKIALALGLLGVYSYRAAVGNVLFPWQHSGRSISKGTGEARFVYIFILGILFTGVKDLLRSQVMSSAVEGRRLKSRGLWEVYSGVVLLVALLFRAHNLPTLACCLLIQTLMAQFIWKKLHYDAAQTTIMHYWFGQAFFYFQGNSNNIATVDISVGFVGLESYVEAPAVFLTAFSTYAGPLLWACHLVCYLSSERDRAALSLGHGSYCFALLRSVPAVFYVVLVTALRYHLFIWSVFSPKLLYEAMHTLVTAAVSLSICAKSAHRKSDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1G3</accession>
    <name>A0AAR2K1G3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SET nuclear proto-oncogene a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1G3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080824.1">
        <property type="protein sequence ID" value="ENSPNAP00000056289.1"/>
        <property type="gene ID" value="ENSPNAG00000035612.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153877"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000003">
        <property type="entry name" value="SET isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000002">
        <property type="entry name" value="Testis-specific Y-encoded-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="145"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="218"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="224"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31653" checksum="D90F6FA2CAA8E939" modified="2024-11-27" version="1">MSASAAKVSKKELNSNHDGADETSEKEQQEAIEHIDEVQNEIDRLNEQASEEILKVEQKYNKLRQPFFQKRSELIAKIPNFWVTTFVNHPQVSALLGEEDEEALHYLTRVEVTEFEDIKSGYRIDFYFDENMYFENKVLSKEIHLNESGDPTPKSTEIKWKPGKDLTNRPSQTQSKAGKKRQHEEPESFFTWFTDHSDSGADELGEVIKDDIWPNPLQYYLVPDMEDEEGEGEDEDEDEEGLEDIDEEGEEDGEEEDDDGEVILDWAEISIHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K607</accession>
    <name>A0AAR2K607_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557685.1">
        <property type="nucleotide sequence ID" value="XM_017702196.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K607"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063521.1">
        <property type="protein sequence ID" value="ENSPNAP00000059748.1"/>
        <property type="gene ID" value="ENSPNAG00000037480.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430045"/>
    <dbReference type="CTD" id="553724"/>
    <dbReference type="GeneTree" id="ENSGT00390000018525"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF1">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="161" mass="17812" checksum="953B06CE50255592" modified="2024-11-27" version="1">MDKLKKVLSGQDGNDDLNVLQAASEATTLGWGTRLKAFIACFALGVFCSVLGSCLLWVPRNGLILFVVFYTFGNVASLLSTMFLMGPLKQVKRMCDKTRALATAIMITCLVLTLCAAFWWKNKGLALLFCVLQFLAFTWYGLSYIPFARDAVIKVFSVCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K7H3</accession>
    <name>A0AAR2K7H3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K7H3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048195.1">
        <property type="protein sequence ID" value="ENSPNAP00000058439.1"/>
        <property type="gene ID" value="ENSPNAG00000033634.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="119" mass="12809" checksum="1E4A145747538DEA" modified="2024-11-27" version="1">YTFGGGTKLSVGSVAAPTLTILPPSSVELQQGKATLVCLANKGFPSDWRLSWKVDGSSWTSGVSQSPGLLQKNDGLYSWSSTLTLQEDQWLKKTVTCEATKDSQPAVSQTLRREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K9J9</accession>
    <name>A0AAR2K9J9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2K9J9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043633.1">
        <property type="protein sequence ID" value="ENSPNAP00000060983.1"/>
        <property type="gene ID" value="ENSPNAG00000031441.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183016"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="12">
        <location>
            <begin position="14"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="11">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="11">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="409" mass="47040" checksum="FB9B5A7B3ACE048E" modified="2024-11-27" version="1">MSVINNAGAGVTIASWNVRGLGHPIKRGRVFSHLKSLKADIIFLQETHIAKTQQSRLRVNWVSQVYQATFTSKARGVAILFRKNIPFHLEAIVSDPQGRYVIVSGHINSFPLTLVNVYGPNFDAPDFFCKLFDAVPEDNFTNIVMGGDFNCFLDPYMDRLSQRPPPVLLSVNVLKNLLNSRNLVDIWRLQHPTQKEFSFYSHVHKSYTRIDYFLVNSELISNVEDTKCHNILISDHCPITLQLKCNIAKEKYSWRFNPLLLKDPLFNKYIMSKLNEFLEINDNGEVSDCTLWESLKVVMRGYVISYEAHKKKTNSDRLLEIQRELTLIEHAHRSSLAQSDYNKILKLKDEYNSILSKYSNLFFDKLVIPQLPEFCKIELDAFPSGKSPGPDGFGSEFYKAFSKQLLPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KAW2</accession>
    <name>A0AAR2KAW2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Oligophrenin 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">OPHN1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KAW2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079086.1">
        <property type="protein sequence ID" value="ENSPNAP00000059619.1"/>
        <property type="gene ID" value="ENSPNAG00000008490.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160157"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01249">
        <property type="entry name" value="BAR-PH_GRAF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04374">
        <property type="entry name" value="RhoGAP_Graf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000001">
        <property type="entry name" value="Rho GTPase-activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000008">
        <property type="entry name" value="Rho GTPase-activating protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047225">
        <property type="entry name" value="PH_GRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF2">
        <property type="entry name" value="OLIGOPHRENIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="266"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="380"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="567"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="641"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="686"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="725"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="807" mass="91915" checksum="287C8F03E733F5D9" modified="2024-11-27" version="1">MGHPPLEFSDCYSDSPDFRERLKCYESELERTSKFLKDVIKDGNNVIGAIRGYSVAVQKFSQTLQTFQFDFIGDTLTDDEMNIAESFREFAGLLQEVEENRMMLVQNACDLLIKPLEKFRKDQIGVTKERRKKFEKESEKYYSQLDKHLNLSSKKKESQLQEADDLLDKERQTFCESSLEYVYQIQQVEDRKKFDIVEPVLAFLQSILTLNNLTVEMTQDFLPYKQELQLSLQNTRNHFESTREEMEELMKRMKLPNPIFIRHGQQPTIEGYLYVQEKWALGMTWVKYYCKYMKDSKLLLLLPTEQKPATKQIPVELTLKSCIRRKSESIDKRFCFDIETQERSTFTVQSISEVNRKQWMEAMDGKEPIYHSPIHKQAEMELNETGFKFVRRCINVIETKGLSQEGVYRTVGSNIQVQKLLNAFFDPKCPGDVDLNSSEWDIKTITSAMKFYLRSLSEPLMTYTLHRELISAAKSENLDYRLGAVHSLVYKLPEQNREMLELLVKHLVSVCSHSCANLMTASNMGVIFGPTLMRAEEETVAAMLDIKFQNIVVEILIEDYKKIFSGPPEESLVPPLPPPRAAPRRRQPITISKRPPRHYSGLQPPAFESREQNGAAHNGPDSGADPTSPVPHQRTKLAPPSVAPPSIPPSSPPALPRSSPPSRAPPQRVSLLPKPELSRLAPRTQDGGQRSDSAVSANGETGVTVGRTQSFQGRKPPPRVTAATKDAESDCLKGRPFVEKSSICRPPTRPPDPPTRCPANQKAPSDDTPPSYVASKTKFFENASRQAGRYVSICETVWAYMYGWVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KDM5</accession>
    <name>A0AAR2KDM5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KDM5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068278.1">
        <property type="protein sequence ID" value="ENSPNAP00000062385.1"/>
        <property type="gene ID" value="ENSPNAG00000037244.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214408"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="13">
        <location>
            <begin position="44"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="341" mass="39370" checksum="5A9A59228DD5A1B2" modified="2024-11-27" version="1">MKTLLRLLEVAYWPSLRKDVWAHCRGCVTCQKYKPRITKLSGLLQSTPVVEPGYMLGVDLMGPLPKSPRQNEYLLVMVDYCSKWVEMFPLRTARAPQIARILVTEVFTRWGTPAYLVSDRGAQFTSHLIHETCREWGVVQKLTTAYHPQTNLTERINRTVKTMIASYVRDKHRLWDRWLSEFRFAINSAWQESTGFTPAEVALGRKLKGPLERLLSRPPDPEQMAYKTIQRQQDLIQQVQANVEKAQAKQARYYNRRRKTEEFSEGDLVWVRTHPLSRADIGFTAKLAGKWSGPAQILKNLNPVNYQIIYPKDPKLIDTVHIEHLKRYFCQAGSPLDGGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KE36</accession>
    <name>A0AAR2KE36_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KE36"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083502.1">
        <property type="protein sequence ID" value="ENSPNAP00000062593.1"/>
        <property type="gene ID" value="ENSPNAG00000037753.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="6">
        <location>
            <begin position="73"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="259" mass="27995" checksum="5F4928EE60BD9ABF" modified="2024-11-27" version="1">SLTHSPHSSIMTSSVTSQVSDMTNTMKRKAGEGIDDQTLPQTFPSKRTRTDGKEVNFSTLTFPQRLWHIWGTCIIDVELFLAEILSQQGELKIFETSVLKSFKRQLNLYGFMKIRSSEVKAKRNSGSKPMTLASTYPSQTPQSGLIEEATPSASKTSLDLGFSQDTSSDAPPMTSSVDSSLDSTLLSLLAKITDLAQTTITDLHLKAAQLQSTLAALIPSMSPSVLADLSSLVSRALVQPCPKCGHNPLCSEDSETAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKP0</accession>
    <name>A0AAR2KKP0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 11">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">UGDH</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 11"/>
    <dbReference type="RefSeq" id="XP_017570518.1">
        <property type="nucleotide sequence ID" value="XM_017715029.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKP0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081551.1">
        <property type="protein sequence ID" value="ENSPNAP00000062977.1"/>
        <property type="gene ID" value="ENSPNAG00000027414.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437725"/>
    <dbReference type="CTD" id="7358"/>
    <dbReference type="GeneTree" id="ENSGT00390000015355"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000001">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000032">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000114">
        <property type="entry name" value="UDP-glucose 6-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028356">
        <property type="entry name" value="UDPglc_DH_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374:SF59">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11374">
        <property type="entry name" value="UDP-GLUCOSE DEHYDROGENASE/UDP-MANNAC DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500133">
        <property type="entry name" value="UDPglc_DH_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="16">
        <location>
            <begin position="332"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="473"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="89"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="130"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="161"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="220"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="267"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="276"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="338"/>
            <end position="339"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR500133-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR500133-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR500133-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="495" mass="54863" checksum="77982509FA89E394" modified="2024-11-27" version="1">MFQIRKICCIGAGYVGGPTCSVIASMCPEIKVTVVDVNEARIKAWNSDTLPIYEPGLDEVVLSCRGKNLFFSTDIDSAIKEADLVFISVNTPTKTYGMGKGRAADLKFIEACARRIVEVSDGYKIVTEKSTVPVRAAESIRRIFDANTKPSLNLQVLSNPEFLAEGTAVKDLKEPDRVLIGGDETTEGQKAIQALCEVYEHWVPKSRIITTNTWSSELSKLAANAFLAQRISSINSISALCESTGADVEEVARAIGMDQRIGSKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVASYWQQVIDMNEYQRKRFACRIIDCLFNTVTGKKIALLGFSFKKDTGDTRESSSIYISKYLMDEGANLHIYDPKVLKEQIIQDLSQPSISGDNPQRVLELVTVTSDPYEACKSAHALVICTEWDMFKDLDYEKIHNQMLKPAFIFDGRRVLDHLHPQLQSLGFQIETIGKKVTTRLPFTPSGGVPRITLNEPPNKKSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNI1</accession>
    <name>A0AAR2KNI1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="25">PARP</shortName>
            <ecNumber evidence="25">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">TNKS2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="25"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNI1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072760.1">
        <property type="protein sequence ID" value="ENSPNAP00000065860.1"/>
        <property type="gene ID" value="ENSPNAG00000002397.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159911"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09524">
        <property type="entry name" value="SAM_tankyrase1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01438">
        <property type="entry name" value="tankyrase_like"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
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        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="14"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="15"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SAM_DOMAIN"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="19 24" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="17 25" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
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    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="16" id="KW-0779">Telomere</keyword>
    <keyword evidence="8 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
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    <feature type="repeat" description="ANK" evidence="24">
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            <begin position="257"/>
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            <end position="1204"/>
        </location>
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        <source>
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        </source>
    </evidence>
    <sequence length="1217" mass="132485" checksum="A9F25CD9E36987F6" modified="2024-11-27" version="1">VIFTLSISTSTQVPDCTLSTSGSYPPPPPHPPTPTYCLHLCASCFARSRSEWTCIYGLGAAAMERAPGREPSRELFEACRSGDLERVKKLLNTANVNSRDTAGRRSTPLHFAAGFGRRDVVDYLLEHGADVYARDDGGLISLHNACSFGHAEVVSLLLHHGADANSRDNWNYTPLHEAAIKGKIDVCIVLLQHGAKPTVRNTDGRTALDLAEPSTKAVLTGKCPTPLLLKNTRFTAAKKLMSLLTPLNVNCHASDGRKSTPLHLAAGYNRVKTVQLLLEHGADVHAKDKGDLVPLHNACSYGHYEVTELLVKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTFLNCHNKSAIDLAPTMLLKERLAYEFKGHALLQAARQADLIRIKKHLSLETINFRHPHTHETALHCASASSYPKRRQVCEMLLRKGANVNEKTKDLLTPLHLASEKSHNDVIEVLLKHEAKVNAVDSQGQTPLHRAARCGQLQTCRLLISAGCDPLITSLQGFAPAQLANESIQQVLQEGALVGNSDTDRQLLEASKSGDLETVKKLCTLQNVNCRDVEGRQSTPLHFAAGYNRVSVVEYLLQHGADVHAKDKGGLVPLHNACSYGHYEVAELLVIHGAVINVADLWKFTPLHEAAAKGKYEICKLLLQHGADPTKKNRDGNTPLDLVKEGDMDIQDLLRGDAALLDAAKKGCLARVKKLCRPNNVNCRDSQGRHSTPLHLAAGYNNLEVAEYLLQHGAEVNSQDKGGLIPLHNAASYGHVDVAALLIKYKACVNAVDKWAFTPLHEAAQKGRTQLCALLLAHGADPTLCNQEGQSPLDLVTADDVRALLTAAMPPSALPACYKPQVISVSASSGMVSSTLSSSSIPHPSGSSLDPQAGAFPEVLGPSGAEGAIGVEKTEEGETVTHLSISQFLHKLGLEHLLEIFDREQISLDVLVEMGHKELKEIGINAYGHRHKIIKGVERLVSGPQSLNPYLTLNTANSGTILIDLASDDKEFQSVEEEMQSTIREHRDGGHAGGVFSRYNILKIQKVCNKKLWERYTHRRKEVSEENHNHSNERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPLHKDRSCYIHLLFCRVTLGKSFLQFSAMKMAHSPPGHHSVSGRPSVNGLALAEYVIYRGEQVPDSSFPASHLLIGSSLTISIVFKPLNCLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L1I3</accession>
    <name>A0AAR2L1I3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M20 dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L1I3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054057.1">
        <property type="protein sequence ID" value="ENSPNAP00000068482.1"/>
        <property type="gene ID" value="ENSPNAG00000028208.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165015"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05676">
        <property type="entry name" value="M20_dipept_like_CNDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF1">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="4">
        <location>
            <begin position="219"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="526" mass="58422" checksum="685A37603A12ED54" modified="2024-11-27" version="1">MVLLLCGAAVHSFRFDELIKYVDDHQEEYVQTLREWVAVESDSSDVTKRAELYRMMDMVGEKLRKMGGKVELVDIGEEQLPDGSSLALPKVVTAQFGSDVNKPTVCVYGHVDVQPAQKEDGWATEPYNLTEINGNLYGRGASDNKAPVLAWIHAVEAFKALDMDLPVNVKYLIEGMEETGSNGLDAMIKSQKSTFFSDVDYIIISDCGWLSKRPALTYGTRGNCYFFAEVEGPKQDLHSGVYGGTVIEPMTDLIGILDTLISPSGTILIPGIRDAVAPFSDEEWKMYQDIEFDVESYREKIGVEQLMYNNKVELLAHRWRHPTVSIHGINGAFSAPGTKTVIPAKVTGKFSIRQVPDMDPAVVEKQVTDHLHSVFAKRKSPNKLKVKMIIGAKPWLADIKHSLYEAGKAAVKRVFNVDADMIREGGTIPIARTFQDVTEKSIIMLPIGGFDDGLHSQNEKMSRVQLSLGMSLTYCRPCEPNSCFVCTGHEWLVAKSHVPVLPKHLPQNTPGNTVECLLQIHKAHVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L1Q4</accession>
    <name>A0AAR2L1Q4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L1Q4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042964.1">
        <property type="protein sequence ID" value="ENSPNAP00000070543.1"/>
        <property type="gene ID" value="ENSPNAG00000032205.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287215"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="52" mass="6108" checksum="6F873A7ACA884E80" modified="2024-11-27" version="1">MLQHDNAQPHVARICTQFLEAENIPVLAWPAYSPDMSLIEHVWDALDWHIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L553</accession>
    <name>A0AAR2L553_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L553"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060221.1">
        <property type="protein sequence ID" value="ENSPNAP00000071675.1"/>
        <property type="gene ID" value="ENSPNAG00000036617.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000180255"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF15">
        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="49"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="140" mass="15989" checksum="E7504D0DDA1B2B8A" modified="2024-11-27" version="1">MELPGRRRRGRPQRRFMDVVKVDMEMVGVKVEDAMDRQHRQLFRTFDWPFVLAQQFRDSCRRWLLSEECDSKEVLDLVVLEQFITRLPEETAAWAQCHQPESMDEATKLSEAHMAAYSGAGASTQQTSLSSLGALPTTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L657</accession>
    <name>A0AAR2L657_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L657"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052961.1">
        <property type="protein sequence ID" value="ENSPNAP00000072020.1"/>
        <property type="gene ID" value="ENSPNAG00000007698.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442698"/>
    <dbReference type="GeneTree" id="ENSGT00940000158300"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF37">
        <property type="entry name" value="ANOCTAMIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="581"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="11">
        <location>
            <begin position="127"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="10">
        <location>
            <begin position="200"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="776"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="803" mass="92329" checksum="0A8369AC6DBC65A1" modified="2024-11-27" version="1">MSTLHLDVPSNQGRKLGHKRAGSIELLEVIGVVKKDNGSGSFSLLPVHSPQSFDYVLVAQKTDDDGQESFRKQRDFINALKDKQLTIQKITDEDKVFYGITAPKEIFETYRYLLKVSDACNWSCEQQGNIPQSTRIRIVDFIVDHTSIESDGEPEYLSDLIQGNIFEAQFCLHEKQEQKELSNSWARWTACLNGQPITDVRDYFGEKVALYFLWLGWYTFLLIPAAVLGVIVFLYGLAFFKTSPLIQEVCQSDTIMCPLCDDKCKIWKLSDTCTYAKVSILFDNEGTVAFAMFMAVWATVFLEFWKRHRASYVSEWKVFDWCEEEEELILEIVNNPNCESKKHSHSYLRSTLVMVLVTFMLLLIIGLAHALVVFRVIASEQLAKFKSWSFISDNSNTVAFMLGAVLHYLTIIIMTKVNRKVALKLCELEKARSYAAIERSFTVKMFVFQFFTLFSSLIYTAFFLGRINGHPGGYVKLAGKWRLEECHPSGCLTDLFIQMAVILFLKQTINNVFEFSGPWFTRWLKRLQSRKLRQNVRQCSKKNCMESTELCDNCKVKDLLRNFELDSVDQFSLFDEFLEMVLQFSFTTIFVAAFPLAPLLALLNNIIEIRLDAIKMVSLERRLIPKKTNDIGIWTDVLETIGVLAVIANGLVIGISSDFIPRLVYRYRYGPCAISSTTEIDCMSGYIQNTLSIANISDENIKNDFLPEQLNIYNGTNVTSCSYRDYRSNEDYTLTNQFWVIQAIRFAFVVLFEHVVVLCKFIAGWFVPGSPLEVKNTRLQDKLDRLKEELKAERERHARSQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L9T4</accession>
    <name>A0AAR2L9T4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L9T4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044292.1">
        <property type="protein sequence ID" value="ENSPNAP00000071454.1"/>
        <property type="gene ID" value="ENSPNAG00000032194.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282498"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="44"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="16856" checksum="714933327CCEF019" modified="2024-11-27" version="1">MKPKLSCLEGAHNVMCGEKEAHQHQNLIPTVKHGGGGIIVWVCFAASGPGRIDVINGKMNSQVYQDILQENLRPSVLQLKLNRRWVMQQDNNSKHRSKSTTEWLHQKKIRHLDRPSHGPDLNPIEMLWHDLKRAIHTKRPKSIAELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LCJ3</accession>
    <name>A0AAR2LCJ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LCJ3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052728.1">
        <property type="protein sequence ID" value="ENSPNAP00000074250.1"/>
        <property type="gene ID" value="ENSPNAG00000005971.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182940"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000009">
        <property type="entry name" value="Synaptic vesicle glycoprotein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000014">
        <property type="entry name" value="synaptic vesicle glycoprotein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.80:FF:000001">
        <property type="entry name" value="Synaptic vesicle glycoprotein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055415">
        <property type="entry name" value="LD_SV2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022308">
        <property type="entry name" value="SV2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01299">
        <property type="entry name" value="synapt_SV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF2">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23894">
        <property type="entry name" value="LD_SV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="594"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="621"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="655"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="682"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="143"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="37"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="711" mass="80315" checksum="3DF5F4173B72F6E8" modified="2024-11-27" version="1">MGMQKTNDDTSLKFYTIFLRFAESQIKSEIMADPYQNSYHHGDPDGYGTYGEGGQDGYGYQTDYPPQEEDAASDVTEGHDEDDQMYEGEYQGIPHPDEIKAARRAARTKARTAVDAMSEQEELAEQYEDIIEDCGHGRFQWSLFMVLGLALMADSVECFVVAFALPSAEKDMCLSNAEKGMLGLIVFLGMMFGAFIWGGMADKVGRRKCLIIALSINCIAAFLSSFAQGYGSFIFFRLFSGIGIGGSVPIVYSYFSEFLQMDKRGEHLSWLCMFWMIGGIYASFIAWGIIPRYGWGFSMGTEFQFHSWRVFVLVCALPAIAALIGLMFMPESPRFLLEHAKHDEAWMILKQVHDTNWRAKGEPERVFTVSQIKTPKTQEDEFFEIQSSTGTAFQRWVVRTVTLVKLVRMCLCDCVLRVQVCLPMFVMLCFSYYGLAVWFPDMIKHLQYEEYESKVKVFHGEKVERFHFNFSLENQVHKGGEYINDKFVNIEMKSVKFESSLFEDCYFEDIKSSDTFFENCTIRSTVFYNTDLYEERFIDCRMENTSFFHTKKGCHLNFAEENDVLIYLVSFLGSLAVLPGNIISALFMDKIGRIRMIGGSMLISAGCTFFLFMSFSQAAIIAWQCLFCGVSVAAWNGIEVITVELYPASKRATAFGLLNATCKLAAILGSSIFASFVGITKVVPILLSCAALVCGGLLALKLPETREKVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LG03</accession>
    <name>A0AAR2LG03_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017541791.1">
        <property type="nucleotide sequence ID" value="XM_017686302.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017541792.1">
        <property type="nucleotide sequence ID" value="XM_017686303.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LG03"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083493.1">
        <property type="protein sequence ID" value="ENSPNAP00000073649.1"/>
        <property type="gene ID" value="ENSPNAG00000035696.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413673"/>
    <dbReference type="CTD" id="393275"/>
    <dbReference type="GeneTree" id="ENSGT00940000166388"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000021">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043183">
        <property type="entry name" value="DNJB2/6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45168">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45168:SF4">
        <property type="entry name" value="SIMILAR TO DNAJ HOMOLOG SUBFAMILY B MEMBER 6 (HEAT SHOCK PROTEIN J2) (HSJ-2) (MRJ) (MDJ4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="319" mass="35289" checksum="A803A4A8C80BB5E7" modified="2024-11-27" version="1">MGDYYQILGVQQDASPDDIKKAYRKQALKWHPDKNPENKEEAERRFKEISEAYEVLSDENKRSLYNRYGKEGLTGGGGRGHYDDHFGSGFTFRNPEDVFREFFGGRDPFAEFFGNDPFDDFFGGGRRHRGMRSRTADSFFGGFGGFPSFGHGFSGFDSGFTSFGPVGGGSFTSFSSSSFGGGGGGGGGGGGGNFRSVSTSTKFVNGRKITTKRIVENGQERVEVEEDGQLKSLTVNACSDRDALEEEQSRYRPSALPGPSSHNRYLRNVAEEDPGDRGLQGLAYSRGLFDNRRKKLWLKEADSKKKRAPRLAPRIGAWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIB7</accession>
    <name>A0AAR2LIB7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">NAD(P) transhydrogenase, mitochondrial</fullName>
            <ecNumber evidence="4">7.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Nicotinamide nucleotide transhydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>NAD(+) + NADPH + H(+)(in) = NADH + NADP(+) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the PNT beta subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the AlaDH/PNT family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LIB7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068420.1">
        <property type="protein sequence ID" value="ENSPNAP00000076298.1"/>
        <property type="gene ID" value="ENSPNAG00000009858.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004624"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:proton-translocating NAD(P)+ transhydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05304">
        <property type="entry name" value="Rubrum_tdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000028">
        <property type="entry name" value="NAD(P) transhydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000002">
        <property type="entry name" value="NAD(P) transhydrogenase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008142">
        <property type="entry name" value="AlaDH/PNT_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026255">
        <property type="entry name" value="NADP_transhyd_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024605">
        <property type="entry name" value="NADP_transhyd_a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034300">
        <property type="entry name" value="PNTB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00561">
        <property type="entry name" value="pntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006942">
        <property type="entry name" value="PRK09424.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160">
        <property type="entry name" value="NAD(P) TRANSHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10160:SF22">
        <property type="entry name" value="NAD(P) TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02233">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12769">
        <property type="entry name" value="PNTB_4TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00836">
        <property type="entry name" value="ALADH_PNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="536"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="604"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="710"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="787"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="865"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="22">
        <location>
            <begin position="67"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="21">
        <location>
            <begin position="215"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1090" mass="114061" checksum="0E3D4513CC5CF68C" modified="2024-11-27" version="1">MSKPSQPGLSGFISKLLHLHCPSDLLISNLVHPRHSQRKSQHLHLRHLQLSLLSFRQSIPYKQLTVGVPKEIFENELRVALSPAGVDALIKQGFNVVVESGAGEAAKFTDEMYSKAGATVQDVRGALASDIVLKVRAPMLNPILGVHETELMKEGATLVSFIYPAQNPELMAQLSDRKATVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKVLIIGGGVAGLAAAGTARAMGAIVRGFDTRAAALEQFKSLGAEPLEVDVKESGEGQGGYAKEMSKEFIEAEMKLFAKQCLDVDILISTALIPGRRAPVLITREMVETMKDGSVVVDLAAEAGGNIETTVPGQLSVHKGVTHVGYTDLPSRLPTQSSTLYSNNIVKLIRAISPDKENFQYDVKDVFDYGTMDHVIRGSVVMQDGKVLFPAPQPINIPVAAPPKPKSVQELEKEKAAAVSPFRSTLTTAGVYSGGLATLMGLGLSAPNAAFTQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLSLMGGGYLPSSTAESLAVLAAFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGVFVGGYGAVLNSGYNIEQMMYLGSGLCCVGALGGLSTQSTARLGNALGMIGVAGGIAATLGVLKPTPELLGQMTAAMAVGGTAGLAIAKKIQISDLPQLVAAFHSLVGLAAVLTCVAEYMVEYPHFATDPAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGVLNSAPLLLPGRHALNAGLMAASVGGIIPYMLDPSYTMGLTCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTSSTGTGKPMEITGTHTEVNVDQSIEMIKEAQNIIIVPGYGLCAAKAQYPIADLVKSLTEQGKNVRFGIHPVAGRMPGQLNVLLAEAGVPYDVVLEMDEINHDFPETDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALSAKVREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIN2</accession>
    <name>A0AAR2LIN2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017550947.1">
        <property type="nucleotide sequence ID" value="XM_017695458.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LIN2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075236.1">
        <property type="protein sequence ID" value="ENSPNAP00000076415.1"/>
        <property type="gene ID" value="ENSPNAG00000033009.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426151"/>
    <dbReference type="CTD" id="563283"/>
    <dbReference type="GeneTree" id="ENSGT00530000063760"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036195">
        <property type="term" value="C:muscle cell projection membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901739">
        <property type="term" value="P:regulation of myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043448">
        <property type="entry name" value="PKHO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15871">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15871:SF1">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY O MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="20"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="325"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="342"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="548" mass="61293" checksum="8C76F8846482623B" modified="2024-11-27" version="1">MKKSHLVKRGLQDANQLSSQPDKVGWIRKFCGKGIFREIWRNRFVMLKGDHLYIFEKEMKNDGKTHEVFDLAEYERSEEVRKAKSHSKKNLSKFTLLRCRQSAARTPNLMFLAVSPEEKESWINVLNTAIIRAKNRVLDEVTIEEESLLAHPTRDRAKIPLGRRLPTRGHLMAVASTSSDGMLTLDLVHEEDASMLEEDYAVWVKDHRGNLDVLAPGRKRAGTDASRPRVTPVEARVKTSSLPRGSELSWSHQSQTHTPQPGKKMLAQGRNRCGSMDDALSRGERRPIKRDGFSHQATLAQSPTSHLQDLINQRLQRTQELLAKVQEQEPHRGRMGSYTHLRGVDSPRLRHLKGSESPHSKSSSSHSSTHGKSVESSPQLKARDSPSSKTKDSPRVKAKDSPGLKSSKSPHSKGNFRSKSIDSADSKGSSSPRIKCIDLTHIKSSDSPLSAGSISPHLKGIDSPNLWLTNSPHFKGLKGTDSPLSEVLDWESRRAAAERLLQEAISSWEEAREVLAEVKELQARQQRAEQSKTAAPSTPSKDDKHESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJR9</accession>
    <name>A0AAR2LJR9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJR9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079108.1">
        <property type="protein sequence ID" value="ENSPNAP00000074576.1"/>
        <property type="gene ID" value="ENSPNAG00000036477.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156902"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF301">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="101"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="270" mass="31392" checksum="8F2B1B0C8B2404CB" modified="2024-11-27" version="1">MMFKNVFGSGFLVRTAHVILTWVITLILFLHDTDLRKQEETGELLVPILFVLLVLISVLLYFAVSLMDPGFVLSDDCDLQFTLGLTEEQQDMIPHTKTLRLRRCGHCLLQQPMRSKHCQTCQHCVRRYDHHCPWIENCVGERNHRWFVLYLVVQFIVLLWGLHMSWSGFSHALTWQLWLKANGVLLATAAVVAVLCLTVLLLLGSHLYLVSLNTTTWEFMSRHRISYLKHCGADENPFDRGALRNLWGFFCVWGTVVWEQVYFRQDSDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LNY0</accession>
    <name>A0AAR2LNY0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acidic leucine-rich nuclear phosphoprotein 32 family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ANP32E</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AZ1 from the nucleosome: removes H2A.Z/H2AZ1 from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AZ1 in the nucleosome. May stabilize the evicted H2A.Z/H2AZ1-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state. Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Multifunctional protein that is involved in the regulation of many processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the ANP32 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LNY0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000088058.1">
        <property type="protein sequence ID" value="ENSPNAP00000078278.1"/>
        <property type="gene ID" value="ENSPNAG00000034219.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182907"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019212">
        <property type="term" value="F:phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000003">
        <property type="entry name" value="Acidic leucine-rich nuclear phosphoprotein 32 family member A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045081">
        <property type="entry name" value="AN32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375:SF5">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32 FAMILY MEMBER E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="13">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="149"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="257" mass="28930" checksum="FEFDB61B5C539E4E" modified="2024-11-27" version="1">MEMKKRISLELRNRTPAEVAELVVDNCRSSDGEIEGLTDEFKELEFLSMVNVGLTSLAMLPSLTKLRKLELSDNNISGSLEILSEKCPNLTYLNLSGNKIKELSTVEALQNLKNLKSLDLFNCEITTLEEYRESIFELLPQITYLDGFDAEDNEAPDSEADDDGNVCNCSDDDEDGEEGAGPVGEYDDEDDDEEGSEGKEVGLSYLMKDEIQDEEDDDDYVEEEEEEEGEEEEADVRGEKRKRDAEDEGEDDDEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LQ73</accession>
    <name>A0AAR2LQ73_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RGS2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LQ73"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086648.1">
        <property type="protein sequence ID" value="ENSPNAP00000078720.1"/>
        <property type="gene ID" value="ENSPNAG00000034364.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157937"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF43">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="63"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="184" mass="21928" checksum="489902DA070381F0" modified="2024-11-27" version="1">MEVTTARLERAGGRQHHPDVATQTRRTSRCRLYVLLKKYTDVKSSRKKSARPTQVEFEQWGQSLEKLLTHQIGLVAFKKFSKSEFCEENIEFWLACEDFRKTKRSEKLAIKAKHIYEEFIRKDSRKEVNLDFYTKEHLGQCLQRPSKNCFEEAQRRIFYLMEISTYPRFLQSDLYHALRAPEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LS42</accession>
    <name>A0AAR2LS42_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-cell immunomodulatory protein TIP C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ITFG1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LS42"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058025.1">
        <property type="protein sequence ID" value="ENSPNAP00000079390.1"/>
        <property type="gene ID" value="ENSPNAG00000021167.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013367"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057089">
        <property type="entry name" value="C2_TIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024881">
        <property type="entry name" value="Tip"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412:SF0">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13412">
        <property type="entry name" value="T-CELL IMMUNOMODULATORY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23122">
        <property type="entry name" value="C2_ITFG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="584"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="T-cell immunomodulatory protein TIP C2" evidence="8">
        <location>
            <begin position="477"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="630" mass="70390" checksum="EFF09A7D97512BC6" modified="2024-11-27" version="1">MFFWCRKAARRTATFVVFAVSPAVRMWPTCCSSLILTLIWAFFVPRFASGLQDVTTDLFGTENEGTVAAFGDFNSDKQTDIFVIRQQSELLIFLADLKAPYFRPKVHLSKDDFPSDISVISSVVPGDYDGDSQMDVLLTGHPKGSDTQQTKVFIFWGNNQTLNQSGRTDLNKTFNDQPLVMDFNGDMIPDVFGVVSDSTEVCYLRNRILKCEKALSTNVKIRVPHSNAFIDLNKDFTADLFLSTVQDSGFGFETWINTDGNFTRNHTEMVPKNVASIGQSAFVDFDGDGSQDHLLPVCFDKTCSKSAVYLARPGQTEWVPVLTDFQRRDGPWGFVPPDSSMAAELQHPITLHLGDYNLDGFPDALAILHNTSNPVQQQAFLLENVPCNNVSCRESGRMFRIHWEQTDLSSVHSAVVATFFDIYEDGILDMIVLSRTDSKTELTIHALKNNFEADAYFVKVIVLSGLCSNDCPEKVKPFGVNQPGPYVMYTSVDSNGYLKNASAGQLSQSAHLSLQLPYTVLGLGRSANFLDHLFVGIPRPPGVKDMRKQEWTAIIPNSQLIVIPFPNSDPRSWSAKLYLTPSNIVLLTAIALIGVCVFILVIIGILHWQEKKADDREKRQEAHRFHFDAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LTL4</accession>
    <name>A0AAR2LTL4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KLC4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LTL4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053079.1">
        <property type="protein sequence ID" value="ENSPNAP00000077987.1"/>
        <property type="gene ID" value="ENSPNAG00000001750.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164764"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000003">
        <property type="entry name" value="kinesin light chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF6">
        <property type="entry name" value="KINESIN LIGHT CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
        <property type="entry name" value="KINESIN_LIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="591"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="85"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="623" mass="70469" checksum="2AE2099F6F7273FE" modified="2024-11-27" version="1">MSTMVYPCEEKLERLTQEEIISSTKLVIQGLEALKSEHNAILHSLVETIKCLNKDEVACLVHEKSSLLRKSVEMIELGLGEAQVMTALANHLSTVEAEKQKLRAQVRRLCQENQWLRDELSNTQQKLQKSEQTVAQLEEEKKHLEFMNQLKKYDKDALPALEEKNGEATKDALDDLFPNDEEEHEQGTSHQPSSAALAAAKRGGYEVPARLRALHNLVIQYANQGRYEVAVPLCKQMLEDLERTSGRDHPDVATMLNILALVYRDQNNYKEASRLLYDAVAIREKTLGEEHPSVAATLNNLAVLYGKRGKYIEAEPLCKRALELREKVLGRDHPDVAKQLNNLALVCQNQGKYEEVEYYYCRALEIYEKRLGLDDPNVAKTKNNLASCYLKQGKYKEAEILYKEILTRAHEKEFGIVDAESKPIWMHAEEREDSKGIAGEDAHYGEQMGWYKACKVNSPTINTTLRNLAVLYRQQGKTEAADMLEEYTLKTRKQVSHRDISGAAVDPLNDANRRRSSSLYSVKYEPGDDGSVEWNGDGTLRRSGSLGKLREVLRRSSEILVKKLQGTIPPESHNPYMKRAASLSYLNNSGDDDAFQSVGSGRRLRESRHLSSSTVELYSGTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M3G6</accession>
    <name>A0AAR2M3G6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Isocitrate dehydrogenase [NAD] subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IDH3G</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M3G6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076629.1">
        <property type="protein sequence ID" value="ENSPNAP00000081634.1"/>
        <property type="gene ID" value="ENSPNAG00000012745.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182989"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000001">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF60">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="391" mass="43250" checksum="087D2C938258AE01" modified="2024-11-27" version="1">SENCANQPHAPLLLTKTGQTVRRPAHSDPDSWRWDRTRTSESCPRALQVWEKGREKRLLVLGDLICLTDWGLHRFCCVPVDFEVVNVDSSLTSEDEINNAVTAIRRNGVALKGNIETNHNLPPSHKSRNNLLRTSLDLYANVMHCQTLPGVQTRHKDIDIIIIRENTEGEYSSLEHENVPGVVESLKIITRVNSLRIAEYAFRQAREKGRRRVTAVHKANIMKLGDGLFLQCCKEVAAGYPDITFDNMIVDNTTMQLVSKPQQFDVMVMPNLYGNVVSNVCAGLVGGPGLVPGANYGKDYAVFETATRNTGKSIANKNIANPTATLLASCLMLDHLGLHDYASMIRKAILRTLSETRLHTADLGGQGTTSEVVQSIMKEVQKGGPLTSEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPV9</accession>
    <name>A0A3B4BPV9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homeobox protein Nkx-3.2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Bagpipe homeobox protein homolog 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NK-3 homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577822.1">
        <property type="nucleotide sequence ID" value="XM_017722333.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPV9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013712.2">
        <property type="protein sequence ID" value="ENSPNAP00000000516.1"/>
        <property type="gene ID" value="ENSPNAG00000005540.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442340"/>
    <dbReference type="CTD" id="503746"/>
    <dbReference type="GeneTree" id="ENSGT00940000165452"/>
    <dbReference type="OMA" id="RFHVQRY"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000225">
        <property type="entry name" value="NK3 homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF34">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX-3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="121"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="123"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="37"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00081047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="252" mass="28518" checksum="3C5CA9D27431617B" modified="2018-12-05" version="1">MASCRASFSIHNILSAELDSPRGLQLSREYTADDISSPELLGKAGGMSCAQDRNEEESRNSRIVPCLLDADTSSDRQSYEEESIGEECSRADSQQELELRLPDTDGEKVDSCNFSPHPPKSGKKRSRAAFSHAQVCELERRFHVQRYLSGPERADLAGALKLTETQVKIWFQNRRYKTKRRQMAAELAATSTGALAKRVAVRVLVKDDQRQYRAEDLHSPHALPFYQSYQYCPYMYCIQPWPSSRSLNGGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRQ7</accession>
    <name>A0A3B4BRQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">26S proteasome AAA-ATPase subunit RPT2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Proteasome 26S subunit ATPase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRQ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002318"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010643.2">
        <property type="protein sequence ID" value="ENSPNAP00000002318.2"/>
        <property type="gene ID" value="ENSPNAG00000008732.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230346"/>
    <dbReference type="OMA" id="HDWLLME"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000067">
        <property type="entry name" value="26S protease regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000007">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000039">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="245"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="28"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002318.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="451" mass="50145" checksum="49E700280F28CF85" modified="2024-11-27" version="2">MIGSRCHVTVWNIVTTTNLAYIRFSFEQGQNQSGGPGPGGGKKDDKDKKKKYEPPIPTRVGKRKRKTKGPDAASKLPLVTPHTQCRLKLLKQERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDSQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPASSAFIQLNVLMTLAGDVTLDDLILAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUM4</accession>
    <name>A0A3B4BUM4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RECQL</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUM4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003342"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008890.2">
        <property type="protein sequence ID" value="ENSPNAP00000003342.2"/>
        <property type="gene ID" value="ENSPNAG00000001339.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011669"/>
    <dbReference type="OMA" id="KLAVCEA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF30">
        <property type="entry name" value="F-BOX DNA HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="9">
        <location>
            <begin position="10"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003342.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="713" mass="81720" checksum="7B4DED748788AB88" modified="2024-11-27" version="2">QDQVTSYSSQVNIQDLPSNLLQAVFSYLPLQQLCLHVRCVCRSWRDIISNPEFLPWRKLYYYYQQGDTDAKQKVQDYLCENDITKENERCLINLWFFLLQAECSLVKLVVLILLLADGVEDMEALHLESSSCPLSHTNLSELLWAVSTLLLAMTHSGIRISSRLHYNVLYVLFQMENTAPPHHMFVSGTGKTNMLIRFSQQRPNLRLLYVTFNKAIATKARRWFPKNVECRTVHSLAYDAVGKRYTVLKKVTEKVKLFFLSKVLPEGKKGYVNAKVVSQTIDTFCQSTDSHISTKHVPLEYKNTNGFIQTTKPHEKREFVKIAKDVWSKMIKEEPREHHAYCITHDGYLKLWQLQKPILKEYDIIMIDEAQDCSPVIMDIWLSQPCAKIFVGDAHQQIYAFKGAVNAMHTIPHTHLYYLTQSFRFGSEIAYAGTVVLDACKKVKRSLLGAKQDGSVCGDDSEVVDSLRSGSGKGDGRIAVLCRKNVTVFSEAARLVQSNPECRIYFIGGVQKLKLKDIEDIWTLKSRGQWENWDRKGIEFRNPFIQHFSRHGGFVALRHYINESEDEDLTYKLTVVEKYSTQIPHLVSRLIASAAKKVQRADFILGTVHKSKGLEFHTVIVADDFKRVPRATSPLQVSLPRDVQEDEWNLLYIAVTRAKRKLFLNKTIISILEMKGVRQESTYLLIFLSQTDLVPLHKDDSSSAAAQFVLVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUT6</accession>
    <name>A0A3B4BUT6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SH2B adapter protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adapter protein with pleckstrin homology and Src homology 2 domains</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">SH2 and PH domain-containing adapter protein APS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SH2B2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Interacts with KIT/c-KIT, SHC1, EPOR, PDGFR, VAV1 and VAV3. Interacts (via N-terminal region) with SHC1. Interacts (via the phosphorylated C-terminus) with GRB2. Interacts (via its SH2 domain) with EPOR, INSR and KIT. Interacts with GRB2 after B-cell antigen receptor stimulation. Interacts (via PH domain) with VAV3. Interacts with NTRK1, NTRK2 and NTRK3 (phosphorylated); after stimulation of the receptor by its extracellular ligand and subsequent autophosphorylation of the receptor. Binds INSR, GRB2, ASB6 and CAP. Insulin stimulation leads to dissociation of CAP. Binds CBS only when SH2B2/APS has become phosphorylated. INSR binding does not depend on the phosphorylation of SH2B2/APS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH2B adapter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUT6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003402"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008794.2">
        <property type="protein sequence ID" value="ENSPNAP00000003402.1"/>
        <property type="gene ID" value="ENSPNAG00000009822.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424055"/>
    <dbReference type="GeneTree" id="ENSGT00950000183191"/>
    <dbReference type="OMA" id="HTHCHTA"/>
    <dbReference type="OrthoDB" id="10047184at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050851">
        <property type="term" value="P:antigen receptor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01231">
        <property type="entry name" value="PH_SH2B_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10411">
        <property type="entry name" value="SH2_SH2B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000008">
        <property type="entry name" value="SH2B adapter protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000187">
        <property type="entry name" value="SH2B adapter protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015012">
        <property type="entry name" value="Phe_ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036290">
        <property type="entry name" value="Phe_ZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030523">
        <property type="entry name" value="SH2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035058">
        <property type="entry name" value="SH2B2_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872">
        <property type="entry name" value="SH2B ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872:SF4">
        <property type="entry name" value="SH2B ADAPTER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08916">
        <property type="entry name" value="Phe_ZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109805">
        <property type="entry name" value="Phenylalanine zipper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0727">SH2 domain</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="583"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="642"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="663" mass="71664" checksum="DE2F963EBC289FED" modified="2018-12-05" version="1">MNGDGAPSSPELSSSCPPPDWKEFCELHARASATDFAHKFRRFVSENPCYDSPGADASFSQHFARHFLDCFAATLSQAREPGSPGSAPKYSIVPFAGLRSCPPPYGHDLYQRRKDAGASSESLDSMDSVGGGGAGGSSTPHKVSALGQSRSSEDVSLGHHHPKARFKKGFSLRNMSLCVMDGVREIWHRRASPEGDRKANGGEAGGPDRWSQKLRLARSSQSHRGAELLEIQREGALRYMVADDTNCVGTAQWQKCRLLLRKTGGASEGGEKFLLEFFVPPKSSKPKVSVPLSAIVEVRTTMPLEMPDKDNTFVLKVENGGEYILETIDSLQKNSWVADIQDCIDPGDSGDDIELASCPHGQPSKEFSMVASCSCELLSEGVHRAPERLSATAADHYSAPTVRCRELPFTQHPSHVPLERFLQLPEAQNASPPPGGPGEPPGEMEGEGSLVGYPWFHGTLSRVRAAQLVLAGGARSHGLFVIRQSETRPGEYVLTFNFQGKAKHLRLSVNENGQCHVHHLWFQTVCDMLRHFHAHPIPLESGGSADITLRSYVQVQRSPTDVPHVPTPPREAPSCRTEAAQLNHPHLPGVPQPPAAPSSDGPLSSSSSSSPTAQPPGGLGGGLHSRSNSAERLLEPPAGGAEEYHDGDGTRRTRAVENQYSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BW23</accession>
    <name>A0A3B4BW23_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BW23"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002781"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009856.2">
        <property type="protein sequence ID" value="ENSPNAP00000002781.2"/>
        <property type="gene ID" value="ENSPNAG00000009138.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157314"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF53">
        <property type="entry name" value="INTERLEUKIN-22 RECEPTOR SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043736189" description="Fibronectin type-III domain-containing protein" evidence="1">
        <location>
            <begin position="31"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002781.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="211" mass="24449" checksum="B097414531E2D8BF" modified="2024-11-27" version="2">MWSAVMAPSEHQRILKVWILLFFVLHQVYCLLPAPQNVTIVSFNLEHKLIWSPGPGTPIFTHFRVQSYRKKKKQWVSLLNCSELSTGESCDLTTYFNNPHCYYFARVQAFIPDQESNWTSSKLFYPILDTILGPPVVECTSCGNCLLLQLSPPPSRGHHRQLEFFFSSYIVNVSRTRDKAQALSVYRVLRFSTGFDVSWTPEHHQHQNENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C368</accession>
    <name>A0A3B4C368_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L30</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C368"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005291"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005533.2">
        <property type="protein sequence ID" value="ENSPNAP00000005291.2"/>
        <property type="gene ID" value="ENSPNAG00000011570.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012138"/>
    <dbReference type="OMA" id="YFQGGNN"/>
    <dbReference type="OrthoDB" id="1928736at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_eL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_eL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="15"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005291.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="118" mass="13127" checksum="CECCCD68900B7286" modified="2024-11-27" version="2">ELAGEKSCLKKSLESINSRLQLVMKSGKYVLGYKQSQKMIRQGKAKLVILANNCPALRKSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRSMPDQQQGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3Q6</accession>
    <name>A0A3B4C3Q6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Eosinophil lineage-specific marker</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017572919.1">
        <property type="nucleotide sequence ID" value="XM_017717430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3Q6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006378"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003669.2">
        <property type="protein sequence ID" value="ENSPNAP00000006378.1"/>
        <property type="gene ID" value="ENSPNAG00000012400.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439181"/>
    <dbReference type="CTD" id="100320899"/>
    <dbReference type="GeneTree" id="ENSGT01150000286973"/>
    <dbReference type="OMA" id="CENDWAR"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01504">
        <property type="entry name" value="PNCREATITSAP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017447855" evidence="2">
        <location>
            <begin position="19"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="18918" checksum="1FD14F9DEA122D2F" modified="2018-12-05" version="1">MWNVIFLVIFMMTQDVSAQGPAVGRKCENDWARFGRTCYKYIAEEKTWAEAEEYCISLGGNLAAIHSSQTQDFLKRLGRSKDGTYFRTWIGAYDGAQDFVWFWSDGSKFDFKAWHSGEPNNKERAEKCLEMNYGDEVLWNDARCSTKLYSVCQTGAKVSFSSSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4G2</accession>
    <name>A0A3B4C4G2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4G2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006782"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002958.2">
        <property type="protein sequence ID" value="ENSPNAP00000006782.1"/>
        <property type="gene ID" value="ENSPNAG00000002379.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160560"/>
    <dbReference type="OMA" id="MMTHIDA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF1">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29236" checksum="389EE7CCBC320E16" modified="2018-12-05" version="1">MFFSGRGRLEGELRNTKIRSSDVSHLRILLHGQIGVGKSCFINSVDSAFQHEIKNKAGEAPCSDKSYTKKYKTYKIENGNGGFLPFVMNDVMGLENKDGVQTADVISALLGHVKEGYKFKSDTPLKECDRNYVSNPSPDDKVHCLVTVVSANSLSLMDQSIINKMKTVKERANDIDIPQIVLLTKIDEVCLLVKQDVKKTYTSKKIQEKINQCSNMIGAPVKFIFPVKNYCYESDTNDPTDILIMLALRHMVSAANDYVASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C558</accession>
    <name>A0A3B4C558_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 23">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017567700.1">
        <property type="nucleotide sequence ID" value="XM_017712211.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000007012"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002558.2">
        <property type="protein sequence ID" value="ENSPNAP00000007012.1"/>
        <property type="gene ID" value="ENSPNAG00000012931.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436008"/>
    <dbReference type="CTD" id="561197"/>
    <dbReference type="GeneTree" id="ENSGT00940000155048"/>
    <dbReference type="OMA" id="GTEASKX"/>
    <dbReference type="OrthoDB" id="6022401at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03122">
        <property type="entry name" value="alpha_CARP_receptor_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17670">
        <property type="entry name" value="R-PTP-G-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17667">
        <property type="entry name" value="R-PTPc-G-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000005">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000016">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000013">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041887">
        <property type="entry name" value="Alpha_CARP_receptor-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF468">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017184295" description="protein-tyrosine-phosphatase" evidence="16">
        <location>
            <begin position="26"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="728"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="20">
        <location>
            <begin position="55"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="343"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="17">
        <location>
            <begin position="810"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="18">
        <location>
            <begin position="998"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="17">
        <location>
            <begin position="1112"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="18">
        <location>
            <begin position="1289"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="524"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1381"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="566"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1407" mass="157093" checksum="5C0FD39756E86F17" modified="2018-12-05" version="1">MRRLLQPCCWRILSFVLSACVLLWADCHPALSGGDQAGQHGWATGDRHRRAAEESYWAYSGAYGPETGWAAAYPECQERNQSPINIADQDTKVSMEYQELTLDGFEAESSNKTSMKNTGKTVAILLKDDYFVRGAGLPGRFKAEKVEFHWGQSNGSDGSEHSINGRRFPVEMQIYMYNSDDFDSLNAAIREKRVIAAMAVFFQVGQKDNPAVDPIIHGLRGVVHHEKETFLEPFVLRDLLPSSIGSYYRYVGSLTTPPCSKVVEWIIFSRPVFLSYKQLEAFYSIFTTEQQDHVKSVEYLRNNFRPLQSLDNREVFKSAVKDAWLPYLTDPYGTEASKACSSAPINMKVLPLNKTALVVRWSRPEITYDPPIISYLISYSWTRNEEAYEETYVKGSDQKLEAVIKPVSPDVLYLFRVQAVCLNDKRSDFSQSMLFRANTTRIFEGTRIVKTGMPTVSPASSADMAPISSGSSTWTSSGLPFSFVSMATGIGPSSSGSQATVASVVTSTLLAGLGFSGGVISSFPSSVWPTTRPPPSTPTTTASSRPAPANPVTSDPEPTADPTSAKAEKEGSDDGEKSGKGEGEEGERDGEEDEDEDEQKEKVKNAPDDVDPQVNSSVTKEPPTAAPDSTAKEKGRVKAENSTAPSSAPEEQVERHTEKAEEEETTVTPGMELGSGGNESVIPQAPESPPRGGKADAGDRSQWPFSIHTERTIVSSMRPVPGSGRMEWIIPLVVVSALTFLCLILLLAVLVYWRRCFQTAHFYVEDSSSPRVVPNESIPVIPIPDDMEAIPVKQFIKHISDLYANNQHGFSEEFEEVQRCTADMKITSEHSNHPDNKHKNRYINIVAYDHSRVKLRPLAGKDSKHSDYINANYVDGYNKPKAYIAAQGPLKSTFEDFWRMVWEQNTGIIVMITNLVEKGRRKCDQYWPTENSEEYGNIVVTLKSSKVHACYTVRRFTIRNTKVKKGQKGSVKGRQCERTVIQYHYTQWPDMGVPEYTLPVLTFVRRSSAAQTPEMGPMLVHCSAGVGRTGTYIVIDSMLQQIKDKVSVNVLGFLKHIRTQRNYLVQTEEQYVFIHDALLEAILGKETEVLSSQLHSYVNSILTPGPSGKTRLEKQFKLVTQCNARFAECFSAQKECNKEKNRNSSVVPSERARVGLAPLPGMKGTDYINASYIMGYYRSNEFIITQHPLPHTTKDFWRMIWDHNAQIIVMLPDNQGLAEDEFVYWPSREEAMNCEAFTVTLISKDRLCLSNEEQIIIHDFILEATQDDYVLEVRHFQCPKWPNPDAPISSTFELINIIKEEAMTRDGPTIVHDEFGAVSAGMFCALTTLSQQLENENAVGVYQVAKMINLMRPGVFTDIEQYQYLYKAMLSLVSTKENGAGPMSIDRNGSMALSDESDPAESMESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAE0</accession>
    <name>A0A3B4CAE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569218.1">
        <property type="nucleotide sequence ID" value="XM_017713729.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017569219.1">
        <property type="nucleotide sequence ID" value="XM_017713730.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAE0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008907"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015098.2">
        <property type="protein sequence ID" value="ENSPNAP00000008907.1"/>
        <property type="gene ID" value="ENSPNAG00000004951.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436955"/>
    <dbReference type="GeneTree" id="ENSGT00950000183183"/>
    <dbReference type="OMA" id="GSADFHC"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000030">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF17">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="357" mass="39801" checksum="6DAC6AD3E88DDB38" modified="2018-12-05" version="1">MSLYSFGLEPLSCHAWNKDRTQIAVSPNSSTVNIYQKKGKEWTKIHELKEHSGRITGIDWAPESNRIVTCASDRNAYVWMLKDGVWKPTLVLVRINRAATCVKWSPQENKFALGSGAKLISVCYFEKENDWWLSKHIKKGICSTVLSLDWHPNNILLAAGSADLHCRVFSAYIKEIEDKPGPTPWGAKMPFGEVLLEHKDCGGWVHSVAFSPSGDSLAWVSHNSAISVADATQGKEVTQLTTDHLPLLSVFYGSETEIVAAGHDCCPYQFSYKGPGKLEFVKKLGLTKQTSKNSMSAMQHFRNLDKKATTDEDDNELNTLHQNSITQLRIVEGQKANVDKFSSVGLDGALVMWDFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CB17</accession>
    <name>A0A3B4CB17_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NRL</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017553181.1">
        <property type="nucleotide sequence ID" value="XM_017697692.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CB17"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008793"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014945.2">
        <property type="protein sequence ID" value="ENSPNAP00000008793.1"/>
        <property type="gene ID" value="ENSPNAG00000014391.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427498"/>
    <dbReference type="CTD" id="4901"/>
    <dbReference type="GeneTree" id="ENSGT00940000166379"/>
    <dbReference type="OMA" id="PCGVNER"/>
    <dbReference type="OrthoDB" id="8961138at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14718">
        <property type="entry name" value="bZIP_Maf_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000016">
        <property type="entry name" value="MAF bZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF35">
        <property type="entry name" value="NEURAL RETINA-SPECIFIC LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="338"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="250"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="400"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="424" mass="45589" checksum="0E659552A75D1A57" modified="2018-12-05" version="1">MSSPSLPLPSLPPSPLAMEYLNDFDLLKFEVKPDTPPPPQQPCSYSKVGLQQQRDTSGSPYVVRPPPDSSLSSSPYTSLPPSPTLSDAHPPPSASSSSSSSLSFPLSVSTGYVSGLSQASQGNQDGSPAPSALPQCPNPASLEDLIWLAALQQQFGGEAGGAASLLGALGGAPERGDRDRGPIGGFLGCEDAVEALLNSAAAAVSSQFPVLSQSSGSSNLGDSSSESGGDVAVSKASELCHRPLLFVSSAPPSLHSANPTTAPYPQPPSPQGRLHHSQHHHHHPHHPMHGNHSHHHHHQLQVSQCAVDERFSDEQLVSLSVRELNRHLRGVSKDEVVRLKQKRRTLKNRGYAQSCRYKRLQHRHALESEKHLLTQQLEQLQCELSRVLRERDAYKARYEKLVNSGEAPPTQANNPPSPPPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CBY9</accession>
    <name>A0A3B4CBY9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prostacyclin synthase</fullName>
            <ecNumber evidence="7">4.2.1.152</ecNumber>
            <ecNumber evidence="6">5.3.99.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Hydroperoxy icosatetraenoate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Prostaglandin I2 synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">PTGIS</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a hydroperoxyeicosatetraenoate = an oxoeicosatetraenoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:55556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59720"/>
            <dbReference type="ChEBI" id="CHEBI:131859"/>
            <dbReference type="EC" id="4.2.1.152"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:55557"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>prostaglandin H2 = prostaglandin I2</text>
            <dbReference type="Rhea" id="RHEA:23580"/>
            <dbReference type="ChEBI" id="CHEBI:57403"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="EC" id="5.3.99.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:23581"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 27 28">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 27">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.152" evidence="7"/>
    <dbReference type="EC" id="5.3.99.4" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CBY9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009462"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015827.2">
        <property type="protein sequence ID" value="ENSPNAP00000009462.2"/>
        <property type="gene ID" value="ENSPNAG00000014941.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153709"/>
    <dbReference type="OMA" id="KFITRMK"/>
    <dbReference type="OrthoDB" id="6692864at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106256">
        <property type="term" value="F:hydroperoxy icosatetraenoate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008116">
        <property type="term" value="F:prostaglandin-I synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000025">
        <property type="entry name" value="Prostaglandin I2 (prostacyclin) synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024204">
        <property type="entry name" value="Cyt_P450_CYP7A1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24306">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24306:SF4">
        <property type="entry name" value="PROSTACYCLIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000047">
        <property type="entry name" value="Cytochrome_CYPVIIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 27" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12 27 28" id="KW-0349">Heme</keyword>
    <keyword evidence="18 27 28" id="KW-0408">Iron</keyword>
    <keyword evidence="22" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="23" id="KW-0456">Lyase</keyword>
    <keyword evidence="20 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 27 28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="9" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="30" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043893233" description="Prostacyclin synthase" evidence="30">
        <location>
            <begin position="25"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="28">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00017409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00033404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00045141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRNR" id="PIRNR000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009462.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="485" mass="55943" checksum="981765B494049E72" modified="2024-11-27" version="2">MIWTILLLFHGILFFFFLSNRTRSKREPPLDKGVIPWLGHALDFGKDAAKFLTRMKLKHGDIFTVRVAGRYVTILLDPNSYDAVLQDTTSLDFTRYAQVLMDRIFNLKLPNHKPDSEKSIMRRHFQGESLTCLNHAMQNHLWTLLTFETTAQNQTNWKQDGLFGFCYSLLFRAGYLTLFGGEQKNNCMDAANVYMEYQKFDSLLIKMAHTTLKSGEKQTAGSVKQRLWELLAQAGLRDNSSLWLQSYRQHLKEEGADEETQRKAMLLQLWATQGNVGPATFWLLAFLLTHSEAMRAMRKEFNSATMPGTITLDPQQHTPVFDSVLEETLRLTAVPFITREVLQNKTLHMADGQEYDVRRGDRVCLFPFVSPQMDPEIHHEPEKFKYDRFLNADGTAKRDFFKGGRRLKYYTMPWGAGTNACVGKPFAIQSIRQFVFLVLSHLDIELSDPEAQVPAVDASRYGFGMLQPIEELQIRYKLRERPCVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CCY6</accession>
    <name>A0A3B4CCY6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-related protein Rab</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">The small GTPases Rab are key regulators in vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Lipid-anchor</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554006.2">
        <property type="nucleotide sequence ID" value="XM_017698517.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CCY6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008319"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000348.2">
        <property type="protein sequence ID" value="ENSPNAP00000008319.2"/>
        <property type="gene ID" value="ENSPNAG00000013904.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427929"/>
    <dbReference type="CTD" id="562524"/>
    <dbReference type="GeneTree" id="ENSGT00940000165121"/>
    <dbReference type="OMA" id="WDSDTVI"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04107">
        <property type="entry name" value="Rab32_Rab38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000222">
        <property type="entry name" value="RAB32, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030697">
        <property type="entry name" value="Rab29/Rab38/Rab32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF43">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008319.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="213" mass="24161" checksum="514B686AED81454C" modified="2024-11-27" version="2">MQQEHLFKVLVIGDLGVGKTSIIKRYVHQIFSQHYRATIGVDFALKVLNWDSQTVIRLQLWDIAGQERYGNMTRVYYREAVGALIVFDVTRASTFDAVLKWKDDLDSKVTLSNSKPVPVVLLANKSDQLREGVCPQIPKLDTFCKENGFVGWFETSAKENTNIEAAARCLVQHILTHEENTVSDSDPDTVTLPSYNNNTKDRDRARCSTCSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFU5</accession>
    <name>A0A3B4CFU5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], mitochondrial</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aldehyde dehydrogenase family 6 member A1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ALDH6A1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Malonate and methylmalonate semialdehyde dehydrogenase involved in the catabolism of valine, thymine, and compounds catabolized by way of beta-alanine, including uracil and cytidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(R)-2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:141212"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:76624"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76627"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62413"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:76628"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-methyl-3-oxopropanoate + NAD(+) + CoA + H2O = propanoyl-CoA + hydrogencarbonate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20804"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:20805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-oxopropanoate + NAD(+) + CoA + H2O = hydrogencarbonate + acetyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:76615"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:76616"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017567321.1">
        <property type="nucleotide sequence ID" value="XM_017711832.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFU5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010842"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017614.2">
        <property type="protein sequence ID" value="ENSPNAP00000010842.1"/>
        <property type="gene ID" value="ENSPNAG00000016460.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435775"/>
    <dbReference type="CTD" id="4329"/>
    <dbReference type="GeneTree" id="ENSGT00940000156110"/>
    <dbReference type="OMA" id="GGAKNHI"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="38"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="525" mass="57198" checksum="A4252F8289877E3B" modified="2018-12-05" version="1">MAATLVRSLAKKRVPLQVGRMCYSSSVPTTKLFIDGKFVESKSSEWLDIHNPATNEVIGRVPKATQEEMLAAVDSCSRAFHSWSETSILTRQQVFLRYQQLIKDNIKELAKLITLEQGKTLADAEGDVFRGLQVVEHTCSITSLMMGETLPSITKDMDTYTFRLPLGVCAGIAPFNFPAMIPLWMFPMGMVCGNTYLMKPSERVPGCAMMLAKLLQDAGAPDGTLNVIHGQHDAVNFICDHPAIKAISFVGSNQAGEYIYERGSKNGKRVQSNMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAILVGEARNWLPDLVERAKHLRVNAGDQPGADVGPLISPQARDRVNQLIQSGVEEGATLLLDGRNIKVKGYENGNFVGPTIISSVTTDMQCYREEIFGPVLVILEADTLDEAIKIINKNPYGNGTAIFTTNGAVARKYTHEVDVGQIGVNVPIPVPLPMFSFTGSRASFRGDTNFYGKQGIQFYTQIKTVTSQWKAEDATLKSPAVTMPTMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CG73</accession>
    <name>A0A3B4CG73_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PALM2AKAP2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017575242.1">
        <property type="nucleotide sequence ID" value="XM_017719753.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CG73"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010982"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017789.2">
        <property type="protein sequence ID" value="ENSPNAP00000010982.1"/>
        <property type="gene ID" value="ENSPNAG00000016606.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440723"/>
    <dbReference type="CTD" id="445815"/>
    <dbReference type="GeneTree" id="ENSGT00940000161837"/>
    <dbReference type="OrthoDB" id="9941155at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498:SF10">
        <property type="entry name" value="PALM2 AND AKAP2 FUSION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498">
        <property type="entry name" value="PARALEMMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="294"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="385"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="438"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="674"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="921"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="977"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1035"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1073"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1109"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1241"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="18"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="87"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <begin position="451"/>
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        </location>
    </feature>
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        <location>
            <begin position="929"/>
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        </location>
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        </location>
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    </feature>
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        </location>
    </feature>
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            <begin position="1123"/>
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        </location>
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        <location>
            <begin position="1172"/>
            <end position="1186"/>
        </location>
    </feature>
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        <location>
            <begin position="1255"/>
            <end position="1272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1272" mass="141451" checksum="FF2C5E0F575A0A31" modified="2018-12-05" version="1">MRRTCPQHTVLLDLSCVMAEAELHKERLQALAEKRKRQAEIEDKRRQLDDLVLQLQHLKSKATRERWLLQGTPAGPHEEDEGRRKQIEQDELNIKKLEDTIHRLESDIIQLENEELQISTKEQVLRERLRETERSIEDLQKSLQNQDGDAVNYICSHIPDVPELNSQTPATASGDHQLPRKPALYAMEISVEKDLKTGGTKILSASPVSPEDAHQRGVKVYDDGRKVIYEVRSGASTTLENGVHPWSSDEVEELMGCVGQSHQRADGTHVLVTPAGPQAKDGQQSYREQTVHKEAKQEMVRGQQGKPQATGQVRQSYEGEVTEPPEATAEKPVTMIFMGYHNVDDEEESKKLLGFDGTIKAEIVLIDEDDEKSLREKTVTDVSTIDGNAADLVSGRPPSEATELSSEGKDESSAKEPPPAGSEKARCVLLTAENGLFPGAALRPSHSPPRRISEDDSELKSEKNLKSVSFLDPVGVISSGNMELETQSESQHNHTTSGQNGVRHTGEALDSEVAQEIRYLDEVLEANYCDPGAEMTSNGTCSLELQTITVEGTGPSVHVSNVSSSVSHDIIVEGNKQTTYVENTLNSTKPNGHSGIINEHSTSPESGTTIKKEARFELRSFHEDKKPSKLFDTPTEKEIRVKKVRPSEEVAELEKERLELIRDQAVKKNPGIAAKWWNPPQEKSLEDELEPEQLESLRRYEERKKKKPEPRDLQTSPKQTVSFVQPDVGYKEDVVVQGIDFSAARQQFLQMEHSKQQQATKRSVAPQIYSAKPFFRTSDVDKSSSSIIVTSHGRVTVEDGDVSQVRGVLCSSGDSTTHTIDNEAKNDDFTCARAVMTIFKDEESDLYQRSMNSCYHPEEIDSGLDDLSLRSQDTTVLETLSNDFSMDNISDSGASNETMSAFLENSAGEYSFPCTPMATTPINGKLEGSTKSPGEQSSSCTGDSLTEEELEYHAGILVQNAIHQAIAHQTDNWQPLQVMQQSSPISERHVEVSEPSAPEEKLPCTPPSKETFPVEEKKATAPKITVQVPTVQPSPAIPVNTYRPPSPSPPPSEKSEFSYFSKYSEAAELRSTASVTRAQDTEVTSGPFKLRSRKQRTLSMIEEEIRAAQEREEELRRERQAQSLRPNPNPTVKQRTNSLPAKLVLTGKTAPGKIEKIRTAPPVSPASSNGAPSPLSDLGSDDSGGSQRPKNFMQTLMEDYESHKVKRREKAEDNSYARLLLANDVTSEVLEATRVTRRKSNMALRWEAGIYANQDEAEEDEEEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CH86</accession>
    <name>A0A3B4CH86_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CH86"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018316.2">
        <property type="protein sequence ID" value="ENSPNAP00000011367.2"/>
        <property type="gene ID" value="ENSPNAG00000017058.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000066148.1">
        <property type="protein sequence ID" value="ENSPNAP00000059904.1"/>
        <property type="gene ID" value="ENSPNAG00000017058.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064158"/>
    <dbReference type="OMA" id="KDYTVMT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
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            <begin position="321"/>
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            <begin position="338"/>
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            <begin position="360"/>
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            <begin position="404"/>
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        </location>
    </feature>
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            <begin position="438"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
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            <begin position="455"/>
            <end position="464"/>
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    </feature>
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        <location>
            <begin position="526"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011367.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="539" mass="60139" checksum="10E34CF63183C788" modified="2024-11-27" version="2">MGKTKTKNQKAARVTSASVAEEEYGTVPHSFVFHRGLVGKNVDQLVTDMRRVMEPYTARALKVRKKNVLKDFVTAAGPLGVTHFLIFTKTETNVNLRLARLPKGPTLFFHVTKYSLIKDVVSSLKRHRMHEQQFSHHPLLVLNNFGLDGMHIKLMATMFQNMFPSINVHTVNLNNIKRCVLLTYDPESKEIDFRHYSLKVVPVGMSRGVKKLMQEKLPNMNKLEDISDLLLQGVNLSESEAEQDGDHNITELPQVYSGRGNMRSQQSAVRLTEIGPRMTLQLVKITEGMGDGNVLYHSIHTKTEEELMEILKRKEARLKEKAERKQKQEQNISLKKQKRDENKKKSLEGMKKKRQQEGAGSDDSEVEDPGMQEDQAAAEESEDEAEYYRQAVGQEPDEDMFPTAKRKHGPGKSPRPFKKRKTSSSKASDEKQSPRPAGKPAFGGKASAGKKFGGKKFGVGGKGFGARKTAGTGSKFGKSKWGKKFGENKRDGFGGNKNGPKKGPSSKLKGQRSKTGSGFKAGPRGGKAKKDFKQKKARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIT9</accession>
    <name>A0A3B4CIT9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ANGPTL3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIT9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011912"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036308.2">
        <property type="protein sequence ID" value="ENSPNAP00000011912.1"/>
        <property type="gene ID" value="ENSPNAG00000003487.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156746"/>
    <dbReference type="OMA" id="GQINTIF"/>
    <dbReference type="OrthoDB" id="8866652at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070328">
        <property type="term" value="P:triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF222">
        <property type="entry name" value="ANGIOPOIETIN-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017257683" description="Fibrinogen C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="244"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="100"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="455" mass="51585" checksum="05A13EFA8998CB6D" modified="2018-12-05" version="1">MAKSKATMKLALLMILIVLTNAILAAENMGTEEPDLPQVGTRSTFAGLDDVRLIANGLLQLGKNLRDFVQKTKGQINDILQKLNIFDKSFYQLSVLASEIKEEEEELKKTTVVLKANNEEIKSLSLEINSKVEEIMMERTQLREQVGGLEEKMSGLSHGLLSADQVAEITALKEVIQAQERSISELLKAVKEQSEHLNYQKNKIKGLENKLNSASLQETTSKFQWDTQTYSMSEYLSNSSTDGSFSSDFPRDCGEVFSTGENTSGLYAIKPNQSEPFLVYCEFTQEGAFTVIQRRHDGSVNFNLSWQSYEDGFGDFRSEFWLGLTKILSIARQGDSLLRLQMEDWKQEKKSTEFQYNLEGPDSNYTIHLKLVHPTRDTDFHAVRFSTKDHNDGYHDESCVRDYTGGWWFSACGDINPNGRCIQSRPRRKGIQWKHNKGQGHLKSSQISIHHAKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CL03</accession>
    <name>A0A3B4CL03_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UQCRQ</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 13">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with UQCC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CL03"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012030"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019211.2">
        <property type="protein sequence ID" value="ENSPNAP00000012030.1"/>
        <property type="gene ID" value="ENSPNAG00000017747.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427892"/>
    <dbReference type="CTD" id="27089"/>
    <dbReference type="GeneTree" id="ENSGT00390000004029"/>
    <dbReference type="OMA" id="MWRRFKG"/>
    <dbReference type="OrthoDB" id="6683853at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.210:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="82" mass="9734" checksum="544D90278B4D3F56" modified="2018-12-05" version="1">MGRHFGDLAKMRHIITYSISPFEQRAFPNYFSKGVPNVWRRFTASIFKIAPPMILMYLTYTWGTHIHEQSKRKSITDLENEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNK0</accession>
    <name>A0A3B4CNK0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Hexosyltransferase</fullName>
            <ecNumber evidence="15">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Golgi apparatus membrane</location>
            <topology evidence="2 15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017557717.1">
        <property type="nucleotide sequence ID" value="XM_017702228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNK0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012938"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020385.2">
        <property type="protein sequence ID" value="ENSPNAP00000012938.1"/>
        <property type="gene ID" value="ENSPNAG00000018755.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430057"/>
    <dbReference type="CTD" id="559521"/>
    <dbReference type="GeneTree" id="ENSGT00940000156219"/>
    <dbReference type="OMA" id="WGDETTF"/>
    <dbReference type="OrthoDB" id="5957813at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF20">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017458925" description="Hexosyltransferase" evidence="16">
        <location>
            <begin position="27"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="329" mass="38248" checksum="78C015B36312C87C" modified="2018-12-05" version="1">MPSKVSCLYLLTVLCWASALWYLSGSRPSTAYVGQMSIPIRKQSPRLLKNMTFSNIRTRTLNPHSFRFLINEPSKCEGTAPFLLILISTNHKEFDARQAIRETWGDESLYSEIRIVTLFLLGHNSEPVLNQMVEQESQIFHDIIVEDFEDSYHNLTLKTLMGMRWVSSFCPNAQYVMKTDSDIFVNMDNLVLNLLRPNAKPRQRFFTGHVINGGPIRDTRSKWYMSREMYPESKYPPFCSGTGYVFSGDMAELIYKTSLHTPLIHLEDVYVGMCLRKLGVHPIQNSGFNHWKMSYSLCRYRRVVTVHQISPEEMHRIWNDMSSKKHLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTU8</accession>
    <name>A0A3B4CTU8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017550652.1">
        <property type="nucleotide sequence ID" value="XM_017695163.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTU8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013949"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037140.2">
        <property type="protein sequence ID" value="ENSPNAP00000013949.2"/>
        <property type="gene ID" value="ENSPNAG00000019850.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426017"/>
    <dbReference type="GeneTree" id="ENSGT00610000087455"/>
    <dbReference type="OMA" id="HEAFKVE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013949.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="261" mass="29662" checksum="0864265F3B35335F" modified="2024-11-27" version="2">MAAVSLTEPLCQPCGYNSRFKVELFVKKPLMPIHLSPEQVALEMLCFCSQLDLLIRAQVHQFQGQLKEDTSPEESESFRKQGAEIIDQMNQCLDHLPKPAPLLEDYLDFAGLSVMFPRVEVYIIHGSPVYMLERPPLDGYFPHLGRLNQLLVLSQQLEDDVKHLGSHKYIAHQLAVIYQVLNSLNDILSLTPIKKDIEANFKQMKMSLVTEEGSKQDPQLPAQYISWISQLTHNIISTVSSLIEELTEELSPVMAFVSKWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CUY9</accession>
    <name>A0A3B4CUY9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CUY9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014561"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022484.2">
        <property type="protein sequence ID" value="ENSPNAP00000014561.1"/>
        <property type="gene ID" value="ENSPNAG00000020512.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154704"/>
    <dbReference type="OMA" id="GCFVFQE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd12086">
        <property type="entry name" value="DD_cGKI-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF68">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0140">cGMP</keyword>
    <keyword evidence="9" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="107"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="284"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="16">
        <location>
            <begin position="541"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="592" mass="67624" checksum="08182CCD171E687A" modified="2018-12-05" version="1">MGTLRDLQFALQLKIEELRQRDALIDELELELDAKDDLIRRLQGELDRLRVTLGQHRAPSLRAKRRAVVTEPTDLDPKLLLQNPPVIHSKSQDSQRVIEAALMENEWLRFVGREQLLALVDCAYSTTAGQGTKVFQEGEEASHTFIVEGRELEDGGKERWKLATVNDKTTFMSIQLIWLSLSLSLSLSLSPALTSSKFWVIERQTFQRIMQRSGILRITQNLDVLRRSYPYYSYRNAHNARPWNNPFTEKHERFSSPQCVIFLCTSIIQCKHNSTHLRDGDRHYTVGMTHSPNRLRQLFRHSNDGSRVFALKIIRKHAVVSPGHRERIFSEKQIMIDAHCAFIVRLYQTFRDGKCLYMLMEACLGGDLWTLLKERGPFDENAVRFYTACVLEALSFLHSQGVLHRDLKPENVLLDHKGYAKLAGFSSAKKLGSLQRVWSFCGSVGYQAPEVILLRGHSATVDYWTLGVFVYELLNSSPPFSGLDQLKVYAAVLRGIDAVEFPNTMSKPVVDLIKRLCRENPTERLGQRNGVKDIRGHKWFEGFDWLGLRDGSHAPPIVPNVHSLLDRGADSSSSENHTDLSPVDDSDWDKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYE6</accession>
    <name>A0A3B4CYE6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Collagen, type V, alpha 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYE6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016375"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024788.2">
        <property type="protein sequence ID" value="ENSPNAP00000016375.2"/>
        <property type="gene ID" value="ENSPNAG00000022616.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155675"/>
    <dbReference type="OMA" id="WWTSRSP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005588">
        <property type="term" value="C:collagen type V trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000001">
        <property type="entry name" value="Collagen alpha-1 type I chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000013">
        <property type="entry name" value="Collagen, type I, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000885">
        <property type="entry name" value="Fib_collagen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF539">
        <property type="entry name" value="COLLAGEN ALPHA-2(V) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01410">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00038">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51461">
        <property type="entry name" value="NC1_FIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0176">Collagen</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043332800" evidence="13">
        <location>
            <begin position="26"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="14">
        <location>
            <begin position="34"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrillar collagen NC1" evidence="15">
        <location>
            <begin position="1043"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="129"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="191"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="557"/>
            <end position="570"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="572"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="597"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
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            <end position="727"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
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        <location>
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        <location>
            <begin position="983"/>
            <end position="1001"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50184"/>
        </source>
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            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016375.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1274" mass="125478" checksum="17073D8CB226FFF7" modified="2024-11-27" version="2">MMTFLHSRIFLFLAVSLAQVLFVKCQDGNSGDDLSCMEDGQVYTNRDVWKPEPCRICVCDSGTILCDDVQCEEVTNCEKVMIPDGECCPVCQNSYPDISGNDSMNYTNINIIKHMSYIIFPKVFTHPSKGPRGPAGYDGEPGVPGQPGEPGPPGHPPGVSTVLNNSSINKRYPSGMRGAQGNVGKPGPMGPLGLSGPSGYPGAPGMKGQPGPTGVRGPEGPQGQRGETGALGRVGPMGLQGPMGVDGGPGAKGPVGSLGPQGPGGLLGPPGPPGPQGSTGQPGIKGSGGDVGVPGFKGEAGPKGEQGPQGAQGVIGPQGEEGKRGPRGDPGSVGPPGPVGERGTPGNRGFPGQDGLQGAKGAQGDRGITGTSGPKGSTGDPGRTGEPGLPGARGLTGTPGVQGAEGKPGPPGGTGEDGRPGPPGPTGTRGPAGSMGLPGPKGFSGDPGKTGEQGSPGAPGQRGPPGKEGEVGPLGAAGPPGPAGDRGEQGPPGMPGFQGLPGPPGPVGETGKPGDQGIPGDGGPVGLVGPRGERGTPGERGELGPHGLPGAKGIPGAPGPDGPKGSPGPPGALGDLGPPGLQGMPGERGISGPPGPKGERGAVGEKGAEGTPGNDGARGPDGQPGVKGEPGEPGQKGDAGSPGPQGLAGPPGPVVGDRNLTNGICTQQTPRQYLKGHGPTGFPGSPGRVGPPGATGPIGQPGPVGPPGKEGPPGLRGDHGPPGRQGERGPPGPAGSPGDKGDPGEDGPTGPDGPPGPAGTTGQRGIVGLPGQRGERGMAGLPGPAGPPGKPGSPGAPGDKGPPGPVGTPGANGPRGDPGPDGPAGSDGPPGKEGVIGPRGDRGDPGPEGLIGPRGAHGPPGPVGATGGPGKRGDPGSRGPVGPPGPAGKRGLPGPQGPRGDKGELGDHGERGQKGHRGYTGLQGLPGPPGTTGDQGPPGIVGPSGQRGPPGPIGPPGKEGYIGQPGPMGPPGSRGSSGDTGPEGPPGEPGPPGLPGSPGPPTAAMDDLFGGPQDYDAGPPPPEFPEDEALPKSNSTELFQADPGVQATLKSLSSQIESMRSPDGSKKHPARTCEDLKQCYPLKKSGEYWVDPNQGSSEDAIKVYCNMDTGETCISANPSSIPRKTWWSSPGNKPVWFGTMTGGTYFTYGNKDQPANSVTVQMTFIRLLSKEASQTITYHCKNTVGYKDEATGNLKKAVVLKASNDLELKAEGNNRFRYTVLEDSCSQANGKWGKTVFEYRTQKTARLPIVDIAPVDIGGPNQEFGIDIGPVCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ48</accession>
    <name>A0A3B4CZ48_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Transcription elongation factor A (SII), 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TCEA3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFS-II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017579577.1">
        <property type="nucleotide sequence ID" value="XM_017724088.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ48"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016071"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024454.2">
        <property type="protein sequence ID" value="ENSPNAP00000016071.1"/>
        <property type="gene ID" value="ENSPNAG00000022199.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443426"/>
    <dbReference type="CTD" id="6920"/>
    <dbReference type="GeneTree" id="ENSGT00940000157034"/>
    <dbReference type="OMA" id="EMDNKKQ"/>
    <dbReference type="OrthoDB" id="44867at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13749">
        <property type="entry name" value="Zn-ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000001">
        <property type="entry name" value="Probable Transcription elongation factor S-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.10:FF:000002">
        <property type="entry name" value="Transcription elongation factor A (SII), 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF50">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR A PROTEIN 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="16">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="17">
        <location>
            <begin position="539"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="15">
        <location>
            <begin position="658"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="82"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="358"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="384"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="425"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="439"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="509"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="700" mass="78993" checksum="6B9E2B775A545D83" modified="2018-12-05" version="1">MTREEELIRTAKKLDKMVSRNNMEGALDLLRELKNFNMTLKLLQDTRIGMSVNAIRKHCTDEDVVSLAKILIKNWKRLLESAHSQKDERPNELKNGRDGSKQTEAPSGASPNRESSPKSILESHKKLTDKHRKEKKEAELKKERQESDHKKEKNSSNHAKERERKDVFHSSVFCRSSPPHPTSSPKCSSVEVKRERRDAPVDFFPPPPSLRIAHPRCPSTEVKKERKEASDPQSSPYPLNLHNPLPPHIRIPKRQPEVKKDRKDAPEILPRTPSQHSHSHPSSPPPPKRPSLEVPKERKKTPDPNAPLPPLPLHLHPPPPQKPSGLEMKKERKEAVDPDAPVPPLPLHLHPPTPTKRPLLDMKKDKEKERKDTSTPKQSAQQRTSEHKKDSRDMGDSKPHKRPSVEMKKERKDSTDFKQQPAKKPIQDGKRERKDSTDSKSGQGQQSSESKPQRRDSTNSKSSSSPPSKKLSGERRDSHGSKSAQSGVLQRKMSTDSNDGRKSKPETPKTPTSPTSPLSPSFSSGAGPLSPRLLTGDSIRDKCIEMLSAALRTDDDYKDYGANCDAMAAEIEDHIYQEIKATDMKYKNRVRSRISNLKDPKNPNLRKNVLGGAIELSRIATMTAEEMASDELKQLRSVLTQEAIREHQMAKTGGTTTDLLQCGKCKKKNCTYNQVQTRSADEPMTTFVLCNECGNRWKFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3E6</accession>
    <name>A0A3B4D3E6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E-selectin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">CD62 antigen-like family member E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Endothelial leukocyte adhesion molecule 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Leukocyte-endothelial cell adhesion molecule 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SELE</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope. SELPLG sulfation appears not to be required for this interaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selectin/LECAM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3E6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028340.2">
        <property type="protein sequence ID" value="ENSPNAP00000018877.2"/>
        <property type="gene ID" value="ENSPNAG00000025355.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164633"/>
    <dbReference type="OMA" id="WIVCIEN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd03592">
        <property type="entry name" value="CLECT_selectins_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000007">
        <property type="entry name" value="L-selectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000176">
        <property type="entry name" value="Selectin P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000001">
        <property type="entry name" value="Selectin P"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033991">
        <property type="entry name" value="Selectin_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002396">
        <property type="entry name" value="Selectin_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF493">
        <property type="entry name" value="E-SELECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00343">
        <property type="entry name" value="SELECTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 23" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0430">Lectin</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5 24" id="KW-0768">Sushi</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C-type lectin" evidence="26">
        <location>
            <begin position="40"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="25">
        <location>
            <begin position="160"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="199"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="263"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="327"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="385"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="449"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="507"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="539"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007360"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018877.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="584" mass="64879" checksum="4C5E3CB2930FB40A" modified="2024-11-27" version="2">MTRHLLITTIIKKRLLCGLLWTIVGLSNRSCRVLPQWTPVAGWSYHYSNTTMDWNTARQWCRDRFTDMVAIQNHGEINHLNTILPRVDGYYWIGIRKINNSWTWVGTNKKLTQEAKNWAKDEPNNGQNNEDCVEIYIKREMDEGKWNDESCTKKKTALCYTASCKKDSCSHHGECVETINNHTCKCFEGFYGEECQHVVKCQAEEISAPDHGSVNCVHPNGNFSYDSQCEYTCNEGYQVVGSQTTQCEASAAWSIKPPTCELVQCSDLTEPLHGTMQCDHLLGSFGYQSSCEFSCEEGYILADSSSSKLMCEATGLWNDSRPICEAVRCPTLEDPANGRIICSGESFGSSCSFSCNDGFSLQGAPHITCTESAQWSQEKPYCEFVQCSDLTEPLHGSMQCQHPLESFGYQSSCEFSCEDGYTLTGSSSGQLVCEGTGHWNDSRPTCEPVRCPTLEDPVNGRMKCSGGSFGSSCSFSCNDGFNLQGATDITCTESAEWSQEKPYCEAIRCQLPDVQAPLNFGCSRSSDDLTVNLTCYFSCDEGFELQGPASTECTVTGEWSTAIPSCTGTVEYHSYTTKSYTTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5J1</accession>
    <name>A0A3B4D5J1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="G_AIG1"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="199"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="242" mass="27350" checksum="B8A605CAB754609E" modified="2018-12-05" version="1">IQGALRIVLLGKTGTGKSSATGNTILGENRFRSEYSYDSVTVQCEIQHAVVEGRNVSVLDTPGINNTHLTELQLVEELRRGANLSSPGPHAFLYIFQHSKSLVKNKQIAEEFEMMFGEEVKKYTIILFVQEDELERGNAFKLCSPLIRLFEQCGRRYHIINNGNQSNRKQVSELLEKIDKLVEQNGGSYFSKEMFELSDFLVFDSFTLQIKHMKKPAGGIINANKSVLHQMINVPLKSLPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D719</accession>
    <name>A0A3B4D719_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ral guanine nucleotide dissociation stimulator-like 3a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017577597.1">
        <property type="nucleotide sequence ID" value="XM_017722108.1"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000019300"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029021.2">
        <property type="protein sequence ID" value="ENSPNAP00000019300.1"/>
        <property type="gene ID" value="ENSPNAG00000003965.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442184"/>
    <dbReference type="CTD" id="100149956"/>
    <dbReference type="GeneTree" id="ENSGT00940000165438"/>
    <dbReference type="OrthoDB" id="26687at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00153">
        <property type="entry name" value="RA_RalGDS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF220">
        <property type="entry name" value="RAL GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="103"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="264"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="5">
        <location>
            <begin position="690"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="604"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="634"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="654"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="799" mass="88596" checksum="FFD4E6C820C3B6EB" modified="2018-12-05" version="1">MRVCVELCAVERGVCVGLQVKKLLRSTGCCSDLQQIGVQLTRFNLLNAEEDTQDPVQEWGEEVEDGVVYGVTLHRICDPADLTDPAHSSESRHRGCMQYRTLKVRRLKAATLQRLVSELVKPECDELDYARIFLSTYRAFTCTSTLIQLLFQREDMISNLDSSVCIRSSLPPLVRLWLDEFWEDFRDSPQHQALRVLCVHLHHRLCFRRLARQAEALLHSLQEEDKRAEIKRSELSSAAAVDRPCEEKLLGPDRVETGSLLGFSARDIAEQLTQLDADLFVRVVPFHCLGCVWSQRDKKENQSLAPTVRATITQFNAVTNWVITSLLCPNSTSAPTSPSTPRSPKSPRSPKSPRPPRSPRSPQMCRSAQCPLTSPVQRARVIEKWISVAQECHQLRNFSSLRAILSALQSNAIYRLKKTWAVVSRESVATFQQLCESFLDENCVLTAREILEEDGAQPSEADSSSAPRSPKLSPSSRHMSCSSGEVPYLGTYLTVLTMLDTALPDTVEGGLINFEKRRREFEILSQIRVLQASCSLYSFPSHPAVCSWLYSCTPLSDQQSYDLSRALEPPVDPCPNSPSFWSQRLLSKKISSLLSSEKAHADQISVSSSGSSSSEMDDLLPLRHKPQSGSLQNISTDSAISSASSSPQPDHCALSSDSASPTPCSSPSPSSSSSSCTALSHSVFNKEVGDSCVIRVSMELDIGNVYKSILITSQDKTSQVVQRALAKHNLENMSCSDFSLVQILAHDKELLLPDKANVFYAMCPSESYDFVLRQRWKSHAKAPVSCSSPAAVPRGRLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7D3</accession>
    <name>A0A3B4D7D3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Early growth response protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">EGR1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547602.1">
        <property type="nucleotide sequence ID" value="XM_017692113.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7D3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018839"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039184.2">
        <property type="protein sequence ID" value="ENSPNAP00000018839.1"/>
        <property type="gene ID" value="ENSPNAG00000025307.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424223"/>
    <dbReference type="CTD" id="1958"/>
    <dbReference type="GeneTree" id="ENSGT00940000165075"/>
    <dbReference type="OMA" id="NFQVPMI"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060059">
        <property type="term" value="P:embryonic retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000092">
        <property type="entry name" value="Early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021839">
        <property type="entry name" value="EGR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021849">
        <property type="entry name" value="EGR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF42">
        <property type="entry name" value="EARLY GROWTH RESPONSE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11914">
        <property type="entry name" value="DUF3432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11928">
        <property type="entry name" value="DUF3446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 16" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="323"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="136"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="525" mass="55685" checksum="BD062EC5FB1CB5FA" modified="2018-12-05" version="1">MAAAKTELLPALQISDPLSFPHSPMDSYPKLEEMIMLSSAGTPFLNASAPEGTGFSSGEAGEQYDHLTGDTLPEIALNCEKSLAEQSYPTQRLPPISYTGRFTLEPAANCSNSLWAEPLFSFVSGLVGINPPPASIPSSSVTQATPSSSAASSSAPSSSVSSSSSSSPSSSASGSSLSCSVHHSEPNPIYSAAPTYSSASPDIFPEPGPGFPSAVSGSSLPYPPPAYPGGKACSASFPVPMIPDYLFPQQQGEISLVPPDQKPFQTQAGQQPSLTPLSTIKAFATQTGSQDLKGVYQSQLIKPGRIRKYPNRPSKTPPHERPYACPVETCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACEICGRKFARSDERKRHTKIHLRQKDKKAEKTAAAVAQNTSVTSVSISAPSPASSYPSPIASYPSPVSSFPSPVASCYSSPVHTSYPSPSIATTYPSASSTFQTPVATSFPSSVASNIYSSPVTTPLPDMQAATLSPRTADIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8W1</accession>
    <name>A0A3B4D8W1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017578548.1">
        <property type="nucleotide sequence ID" value="XM_017723059.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8W1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019546"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029430.2">
        <property type="protein sequence ID" value="ENSPNAP00000019546.1"/>
        <property type="gene ID" value="ENSPNAG00000006524.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442826"/>
    <dbReference type="CTD" id="3268"/>
    <dbReference type="GeneTree" id="ENSGT00940000155511"/>
    <dbReference type="OMA" id="CFDCGMR"/>
    <dbReference type="OrthoDB" id="6036at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007289">
        <property type="term" value="P:spermatid nucleus differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08838">
        <property type="entry name" value="ArfGap_AGFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000005">
        <property type="entry name" value="Arf-GAP domain and FG repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052248">
        <property type="entry name" value="Arf-GAP_FG-repeat_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134:SF6">
        <property type="entry name" value="ARF-GAP DOMAIN AND FG REPEAT-CONTAINING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134">
        <property type="entry name" value="DRONGO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="24"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="420"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="372"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="486" mass="52228" checksum="1F7F6578A3FC0895" modified="2018-12-05" version="1">MSVCHRDSSSMSNRKHRDSQEICARKVRELAHIGANKQCFECSQPGVTYIDITVGCFVCTSCSGMLRGLNPPHRVKSISMTTFSQQEVEFLQNHGNEVGKRTWLCTFDRKTDGCFDTRDSQKFKEFLQDKYERKKWHFSKSKARRDVEGPWSPGVQPIPAPHGPAQSQPPPGHPLNPTARPTRTLSQSQMSTWDRAPAVSPADVRTEVFTARPTRSQSFRDPPVKDSLLSGMERQRPGTISPAMGPQSRLPSFPALPRPSASSTFKNSFTLGRTVSSGGGAPFRAFPKSLSLDFGGLGPSHSAQTPPAPPPVSQDRYAAVSQLDKVFADNTPATVTSTAPSCGPPQYNTLFGNRLSSSSTPASSPGVPEATSAPQTFANFPNPFASTASSSQPALSPSNPFNSTATGDCSTLVSPSPSAVFPHSASFPAPSTQNAVPHEPANNQEANGFTSFPVPEATSKVQRSMSVNPFTGNVYPTRGASLNPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D903</accession>
    <name>A0A3B4D903_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Inositol oxygenase</fullName>
            <ecNumber evidence="4 15">1.13.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 15">Myo-inositol oxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MIOX</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>myo-inositol + O2 = D-glucuronate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="EC" id="1.13.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13 15">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the myo-inositol oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.99.1" evidence="4 15"/>
    <dbReference type="RefSeq" id="XP_017570177.1">
        <property type="nucleotide sequence ID" value="XM_017714688.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D903"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019591"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029527.2">
        <property type="protein sequence ID" value="ENSPNAP00000019591.1"/>
        <property type="gene ID" value="ENSPNAG00000026257.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437510"/>
    <dbReference type="CTD" id="55586"/>
    <dbReference type="GeneTree" id="ENSGT00390000016211"/>
    <dbReference type="OMA" id="RYNTKYG"/>
    <dbReference type="OrthoDB" id="5151075at2759"/>
    <dbReference type="UniPathway" id="UPA00111">
        <property type="reaction ID" value="UER00527"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050113">
        <property type="term" value="F:inositol oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007828">
        <property type="entry name" value="Inositol_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588:SF0">
        <property type="entry name" value="INOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588">
        <property type="entry name" value="MYOINOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05153">
        <property type="entry name" value="MIOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 15" id="KW-0408">Iron</keyword>
    <keyword evidence="7 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="86"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="142"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="221"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="14">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="286" mass="33640" checksum="08313EED2FEB59A2" modified="2018-12-05" version="1">MRVINLGPDPSLAYRPEYPLQEGKDKSQFRNFESGDLFERVFNTYKLMHKHQTLDFVKQKHAEWAGCTHSRMTVMESIDRLDQLIDESDPDVDFPNSFHAFQTAEGIRREHPDKDWFQLVGLIHDIGKIMALWGEPQWAVVGDTYPVGCRFQNAIVFRETTFVDNPDDKNSSLNTECGIYETHCGLGNVLMSWGHDEYLYQVMKFNKCPIPEEGLYMIRFHSFYPWHTHGNYMHLCNEKDLKMLPWVKEFNKFDLYTKSTDLPDVEQLKPYYQSLIDKYCPGVLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAQ7</accession>
    <name>A0A3B4DAQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DAQ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021427"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032710.2">
        <property type="protein sequence ID" value="ENSPNAP00000021427.2"/>
        <property type="gene ID" value="ENSPNAG00000037281.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012710"/>
    <dbReference type="OMA" id="TRKHYCN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF1">
        <property type="entry name" value="PERFORIN 1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043949465" description="C2 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021427.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="130" mass="14551" checksum="43439A6AD79666E7" modified="2024-11-27" version="2">MTPRLLCMAVLVLAAHLALTKAAVRVWGIQASGLEGDPTGPPDPYIKVWCGSSFGGMTEFYRDTQRPSWNAEFRFSNCKANDILKLEVWDKDLNLDDHLGTCTTQVQRGLNRNKSCRVGKGSLTYNFLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DB43</accession>
    <name>A0A3B4DB43_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2 11">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DOT1L</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="2 11"/>
    <dbReference type="RefSeq" id="XP_017572296.1">
        <property type="nucleotide sequence ID" value="XM_017716807.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000021607"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033067.2">
        <property type="protein sequence ID" value="ENSPNAP00000021607.1"/>
        <property type="gene ID" value="ENSPNAG00000028725.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438776"/>
    <dbReference type="CTD" id="84444"/>
    <dbReference type="GeneTree" id="ENSGT00390000013515"/>
    <dbReference type="OMA" id="ARGDCGQ"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20902">
        <property type="entry name" value="CC_DOT1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.60:FF:000001">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-79 specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000033">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-79 specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="14">
        <location>
            <begin position="16"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="333"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="653"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="761"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="860"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1092"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1397"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="557"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="763"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="785"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="811"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="865"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="924"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="950"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="983"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1000"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1044"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1068"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1121"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1147"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1168"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1225"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1439"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1473"/>
            <end position="1515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1570" mass="169715" checksum="57785BB84D043B72" modified="2018-12-05" version="1">MGEKLELKLKSPAGAEAAGYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEIEMMEDDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKAEIPATYAEAMDREFKRWMKWYGKKHGEYTLERGDFLSEDWKERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIATIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQKDTKENKSNTTTPTKPKEQRDSGGEEESSGPLVPVKPSPKPRRAKLLAKGRKLGNKKRGRPKKASAAAAAAAERKTKKSQSALELLHAKTLSAAPPQDGYRSPQSPFYQLPPKVQHYTASQLLLGSVPTGLQTLLDNIKVQYLQFMAYMKTPQYRTNLQQALEQERLRHTELSGQAQQLMNACHAHKERIKDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLERDMAELKDQSLMLLKARCEELKLDWGSLCLETLLKEKAALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEDSPYRKSLPSLEPRPPLDTDTPKLAGLNGLSPELSINGTASPSYERGSGGGKNELLSRYLPISPDHEIVPPTPDSRGRQVGQPLPDYTRFSPAKIALRRHLNQDPSTGQFRSLGSIHRGDVGAVSSPTLSLKQGCHSPSASDNQQITTPKSAERMSKEKSPAGPGDNITSLPISIPLSTVHPNKLPVSIPLASVVLPSRAERLRNTPSPVSSREQSAGHSNSKQPLTPNSGYSSHSGLVNGSHSAMLTGDQDCDALSPLPHSTPLLGPQSRGPGLSPPLGTGGVLQYADRPPRLPLEEGPERQGPESDTELLDSEARRRIFFSSSSSSSSSSSSSAGGSRLHHGSSKPHHQGSHHHHHHHHHHHHSPGSHGQEGRKRGRRKRSSSGVPPASGSPKRRSFPSLGGAPHTSGSPLNINTMVNNINQPLEIAAISSPENSGRSPREADLDQPPILKRERPLELNGSSRYSSTPSSEDEDAGYPADSSSSRIERKIATISLESRDGDRGSQGRKSGASSVSSEVSSSSSKWKSTFSPISDPKPTPPDLRQGGSPFGMSRGTGGGTDSDSDQKQQRRAERERSEPYGNPKAFLGSEAGGRSTALSERQQQKQKPREWELKAVSGLASQNLFISAATSGGILSGKVGGANTVSSASSVGQYFPLGGASVLQSLFGAQTSSPATSGATRLVNGHSALGSFSSAGLAGGAAGGIFHHMVPSSVSSQQSGATLVSPGALGSLLSLSSSQQRHPDAKSSGAPSFLASGSSVFPPPQTQHGRTQTVLHAPSPPTLSLPPPPPLLNVSASSSAPPQPSPPRSERQQSASLQTLRAVPSASSNSGISPASAACATATSTASSLSSTSSRPFTVHYQPHLLPPATPSSSSSGGSSSGGGGVTSWRTLGMQASYAASQVPSARPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDC3</accession>
    <name>A0A3B4DDC3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PYROXD1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Probable FAD-dependent oxidoreductase; involved in the cellular oxidative stress response. Required for normal sarcomere structure and muscle fiber integrity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. PYROXD1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552468.2">
        <property type="nucleotide sequence ID" value="XM_017696979.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552469.2">
        <property type="nucleotide sequence ID" value="XM_017696980.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDC3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022427"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041218.2">
        <property type="protein sequence ID" value="ENSPNAP00000022427.2"/>
        <property type="gene ID" value="ENSPNAG00000029801.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427061"/>
    <dbReference type="GeneTree" id="ENSGT00390000014894"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041575">
        <property type="entry name" value="Rubredoxin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF2">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18267">
        <property type="entry name" value="Rubredoxin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="12"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="300"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-rubredoxin oxidoreductase C-terminal" evidence="10">
        <location>
            <begin position="411"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="213"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022427.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="500" mass="55429" checksum="259E5BBB800511A4" modified="2024-11-27" version="2">MAGTSESEKRFRFVVVGGGIAGVTCAEQLALQFPSEDIALLTAAPLVKTVTNFRQVSKTLQDFSVEEQPASILEEKYPNLKVIHSAVKGLHAKQHVLQTENRQVFHYEKLCLCSGARPKLLIPDNPNVLGIRDTDSAQEFQKHLSKAKRMVVVGNGGIALELVYEVEGCEVIWAIKDKAIGNTFFDAGAAQFLIPSLEADKPEKALPCKRTRYTTASKDDHSQSSDVSSQGSTAEGSGELGSALGPDWHEGISLRGTEEVSHRVHVEYECEIKTIYSQQDFMKSPLHTQETDLGLWPVYAHLTNGKTFGCDFIVSATGVVPNTEPFLHGNNFELADDRGLKVNEHMLTSVPDVYAAGDVCTAGWEPSALWQQMRLWTQARQMGWYAARCIVADVLEEPIELDFCFELFSHITKFFNYKVVLLGKFNAQGLGSNHELLVRCTKGHEYVKVVLSGGRMMGAILIGETDLEETFENLILNQMDLSSYGEELLNPNIDIEDYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEM2</accession>
    <name>A0A3B4DEM2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. RasD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548387.1">
        <property type="nucleotide sequence ID" value="XM_017692898.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEM2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021511"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040612.2">
        <property type="protein sequence ID" value="ENSPNAP00000021511.1"/>
        <property type="gene ID" value="ENSPNAG00000005318.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424712"/>
    <dbReference type="GeneTree" id="ENSGT00940000163566"/>
    <dbReference type="OMA" id="DFRPPMN"/>
    <dbReference type="OrthoDB" id="265044at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04147">
        <property type="entry name" value="Ras_dva"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000475">
        <property type="entry name" value="GTP-binding protein Rhes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF9">
        <property type="entry name" value="SMALL GTPASE RAS-DVA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="207" mass="23548" checksum="DF3CFEAEF9BE060F" modified="2018-12-05" version="1">MSLDVKEKTQVRLVFMGAAGVGKTSLIRRFLQDSFESKHRRTVEELHSKDYEIAGVKVTVEIMDTSGSYSFPAMRKLSIQSSDAFALIYAIDDPESLEVVKSLRDEILEIKEDKLTPIVVVGNKTDKTDRQVQADDVLSMVELDWNNSYLETSAKENENVVEVFRELLQQANLPSRLSPALRRRRETFPKDTDFRPPMNKTNSCTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFB3</accession>
    <name>A0A3B4DFB3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">One cut domain family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CUT homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFB3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021761"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040759.2">
        <property type="protein sequence ID" value="ENSPNAP00000021761.2"/>
        <property type="gene ID" value="ENSPNAG00000028955.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183103"/>
    <dbReference type="OMA" id="KFHQHAV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="entry name" value="CUT_dom"/>
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        <property type="entry name" value="HD"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
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        <property type="entry name" value="ONE CUT DOMAIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057">
        <property type="entry name" value="TRANSCRIPTION FACTOR ONECUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="13">
        <location>
            <begin position="265"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="365"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="367"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="212"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51042"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021761.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="449" mass="49406" checksum="935E1688E359DAFE" modified="2024-11-27" version="2">MNCTDLTMENIGNLHAVSHAHPPGDLMSSPHARAPPSHRSLSHGRSAMVSGMASLLDGAGEYRAEPSLHAAMSVPCDSSMSLSGTYTTLTPLQHLPPISTVSEKFPHHPHHQHQHHQPHHPHAHQRFSAGNVSGSFTLMRDDRALASVGDLYGHYTKDVTTVGPSLSPLPSSLGALHGATQQPLAAYGDKLLSSGSFEAHAAMLGRGDEHMARGGLGAHGPGLASALNGVHHHHHGHHHHHHHHPHHGHHSHSHGNDRHAPGGGGGSGGQAEEIDTKEVAQRITAELKRYSIPQAIFAQRILSRSQGTLSDLLRNPKPWSKLKSGRETFRRMWKWLQEPEFQRMSALRLAACKRKEQEQLKERNSVPKKQRLVFTDLQRRTLIAIFKENKRPSKEMQVTIAQQLGLELNTVSNFFMNARRRCVDHWHHDLQHGHSPGQPGTSAPTFSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGR8</accession>
    <name>A0A3B4DGR8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Aminopeptidase</fullName>
            <ecNumber evidence="23">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21 23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21 23">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Heterodimer with ERAP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 23">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="23"/>
    <dbReference type="RefSeq" id="XP_017546566.1">
        <property type="nucleotide sequence ID" value="XM_017691077.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGR8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022104"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040989.2">
        <property type="protein sequence ID" value="ENSPNAP00000022104.1"/>
        <property type="gene ID" value="ENSPNAG00000004748.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423642"/>
    <dbReference type="CTD" id="64167"/>
    <dbReference type="GeneTree" id="ENSGT00940000162653"/>
    <dbReference type="OMA" id="GGWDEMT"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000046">
        <property type="entry name" value="Endoplasmic reticulum aminopeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000003">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF239">
        <property type="entry name" value="ENDOPLASMIC RETICULUM AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3 23" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0391">Immunity</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 21 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 23" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 23" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 21 23" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="26">
        <location>
            <begin position="97"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="24">
        <location>
            <begin position="318"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="25">
        <location>
            <begin position="640"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="22">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00063803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="981" mass="112598" checksum="156EFC65440261E8" modified="2018-12-05" version="1">MQVGLTASSDKTSSQECERSCHCKYDYHSPNKSAVFHSTVMSSSSVVYLLLTLISLTLPSTCLPSESSSTATENTHVATNGLPFPWSKLRLPNYIIPVHYHLLIHPNLTTLRFNGSVKIEIDVKNNTNWVVLHSKNLEITAATVLDESEANLFDKALPVLDYPPHEQIAIFSPTILTSGEKYFLYLEFSASLNDGFFGFYKSSYKTTEGQTRVLASTHFEPTSARMAFPCFDEPNFKANYSVRIRRGPSHIALSNMPIEHTVELDNGLFEDRFEASVQMSTYLVAFIVCDFKSVSKRTVTGIDVSIYAVPHKWHQTHYALEAAVKLLEFYETYFNIYYPLPKLDLIAIPDFQSGAMENWGLTTYRETSLLYDPDVSSTSDKLWVTMVIGHELAHQWFGNLVTMEWWNDIWLNEGFARYMEFVSVEATYPQLKVEDYLLDTCFAAIGRDSLNSSRAISCSVENPTQIKEMFDTVSYDKGACILHMLRHFLTDDVFQSGIVRYLRKFKYRNAKNEDLWDSLVNTCSEEDFTSGQYCYSDTQATKNAYRFVGEHIDLKKMMCTWTLQKGIPLVTVKRQGTKLYIEQERFLKTVQPSDPLWQSMQTGYLWHIPLTYKTSHSNNEMRHILQIKSDVLVLDEDVDWVKFNTDMNGYYIIHYDAEGWNVLIELLKVNHSAVSFKDRANLIHNAFQLVTAGRLTLDQALDLITYLKSETHNVPLLQGLGYLESFYKMIEKRNIADITQNLKTYILQYFRDVIDKQTWSNEGTVSDRRLRSELLSLACDLGYTPCLEKAKHFYQTWVTSNGTISLPSDVTETIYMVGAQDENGWQYLLEKYGVSMSEAEKCKFLAALASSKDSSKLLRLLELGMEGSVIRTQDLSTLIYTVARNPAGHFLAWNFVKKNWNKLVEKFQLGSFSMRNIIIGTTAQFSSKEELANIKDFFDSVKDQSSQLRVTQVAVENVEKNILWLERNLETLRMWLQQRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DI27</accession>
    <name>A0A3B4DI27_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Acid-sensing ion channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Amiloride-sensitive cation channel 2, neuronal</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Forms voltage-independent, pH-gated trimeric sodium channels that act as postsynaptic excitatory receptors in the nervous system, playing a crucial role in regulating synaptic plasticity, learning, and memory. Upon extracellular pH drop this channel elicits transient, fast activating, and completely desensitizing inward currents. Displays high selectivity for sodium ions but can also permit the permeation of other cations. Regulates more or less directly intracellular calcium concentration and CaMKII phosphorylation, and thereby the density of dendritic spines. Modulates neuronal activity in the circuits underlying innate fear.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037388088.1">
        <property type="nucleotide sequence ID" value="XM_037532191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DI27"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023098"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013509.2">
        <property type="protein sequence ID" value="ENSPNAP00000023098.1"/>
        <property type="gene ID" value="ENSPNAG00000007637.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412602"/>
    <dbReference type="CTD" id="407672"/>
    <dbReference type="GeneTree" id="ENSGT00940000164727"/>
    <dbReference type="OrthoDB" id="6021021at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.470.10:FF:000001">
        <property type="entry name" value="Acid-sensing (proton-gated) ion channel family member 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.820:FF:000001">
        <property type="entry name" value="acid-sensing ion channel 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3590.10:FF:000002">
        <property type="entry name" value="acid-sensing ion channel 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000001">
        <property type="entry name" value="Acid-sensing ion channel subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3590.10">
        <property type="entry name" value="acid-sensing ion channel 1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004724">
        <property type="entry name" value="ENaC_chordates"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00859">
        <property type="entry name" value="ENaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF222">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00038517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00069596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00077346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="608" mass="68205" checksum="60BEDFDD4DF409BF" modified="2018-12-05" version="1">MNSHSARALWPSLPHTHAHTRTGRVHTASPSQHQRARGKSPRACSPSTAPPRAQSRDARCAPALRTAPMRVSGARVSAREAGRAFLQRTTLHGVRHVFLAASPPRRAAWLLAFVASLALLFTWSSNRVRYLLSAPVYTRAHVTHARRLTFPAVTICNRNLVRPRRMTKPDVFGVGAWLGLIGRNGRLTAAAREALAQTPAPTVGSRPGTSTGYEPPWSPLSRMLDFEHFLPPPRDAQPTMRQLLDRLGHQLEEMLLQCRFQGELCGPRNFSTIFTRYGKCYTFNSGQDGRPLLVTMKGGMGNGLELMLDIQQDEYLPVWGETDETSFEAGIKVQIHTQDEPPFIDQLGFGVAPGFQTFVSCQEQRLTYLPPPWGDCRDAPMDSDFFSTYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVERDNDYCVCETPCNMTRYGKELSFVKIPSKASAKYLAKKFNKSEQYISDNILVLDIFFEALNYETIEQKKAYELAGLLGDIGGQMGLFIGASILTILELFDYLYEVIKYKLCRCAKKSHKRNNNNDRGAVLSLDDVKRHAPCENLRTPSTYPANILPHHPGQGNFEDFTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQE1</accession>
    <name>A0A3B4DQE1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger and BTB domain containing 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZBTB1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017548082.1">
        <property type="nucleotide sequence ID" value="XM_017692593.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQE1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025169"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008281.2">
        <property type="protein sequence ID" value="ENSPNAP00000025169.1"/>
        <property type="gene ID" value="ENSPNAG00000010111.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424522"/>
    <dbReference type="CTD" id="22890"/>
    <dbReference type="GeneTree" id="ENSGT00940000157501"/>
    <dbReference type="OMA" id="CAFACAN"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18192">
        <property type="entry name" value="BTB_POZ_ZBTB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF42">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017252482" description="Zinc finger and BTB domain containing 1" evidence="9">
        <location>
            <begin position="34"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="134"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="655"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="683"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="763"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="485"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="501"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="790" mass="88731" checksum="C90AA2EF23EDE9B2" modified="2018-12-05" version="1">MYGCIFTGCPWPHGRVRAGCVFSFLFFSPLAFACAFACANSRSGCAEVLSSLLLLLLLQKNEGAISSPRRPNFPRAFQAKEAAKARGSASQLHFFARHRSAHVNLVLCRVMARPSHSDHVLQQLNNQREWGFLCDCCIAIGDIYFRAHKAVLAACSSYFRMMFIRDQQGTARLDLSNMQISAECFDLILQLMYLGRIMVGHFEFEELKSSMVYLQMYYIPDSLEDLRDIRTSNLTPSSSASSSSSSSSSSSSAVGGKMMFGVRMFEQRRQSPPDSEHVSKLANTTTRQNINISTVRPVEDVVLTLAPSHSENLVEQPCDLRKRTSGRSSTLKERPRFGRTYTCDDCGFVFSCEKLLIEHILTCTNRKAYQTPRASKAADNDSSKAESSASEGGEEHRAVCKGEEDWPEHKSDPESVIRSVATGMDTDPALSRNITIKVEQEEDSDTEMENIKVVQVGNHSVGSCKVRRRTFKANVTDHVRFDSEEEAGVSTMDDGASALEGHSKETGSEDKVHRIKEEKQDGECTPCELCGALLTEEDQSSHYISNHMGHICACGRCGQVLIKGRQLQEHAERCGEPQCAETDSPGEDSPLPEEGQAMEEGLLEGADLGFRCPLCGLIFESESQAVEHTLACPEQEPFRPAMMEDSSEPDHRRKHFCAICGKGFYQRCHLREHYTVHTKEKQFTCQTCGKQFLRERQLRLHTDMHKGMARYVCPVCDQGTFLKHDHVRHMISHLSAGETICQVCFQIFPSGEHLEKHMDVHLYICGVCGEKFRLRKDMRSHYNMKHTKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTH4</accession>
    <name>A0A3B4DTH4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTH4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005542.2">
        <property type="protein sequence ID" value="ENSPNAP00000026279.2"/>
        <property type="gene ID" value="ENSPNAG00000005074.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164173"/>
    <dbReference type="OMA" id="KIVVYVM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF287">
        <property type="entry name" value="CADHERIN-RELATED NEURONAL RECEPTOR VARIABLE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043556992" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="27"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="688"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="31"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="131"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="240"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="348"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="453"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="576"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026279.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="806" mass="87029" checksum="FD462BED537BEF10" modified="2024-11-27" version="2">MVSPGQRKRLWTSCVASLCLLELCAAQMFYSISEEISKGTFVGNIAKDLSINAHELESRGLRIVSGNSNRYFDVNSKTGVLFVNEKIDREQLCGNAEKCSVNIEAILNDPMQLHRIEVNILDLNDNAPSFHEISYTINITESAYTGERFPLPVASDADLASNSVKSYKLSPNEHFSLDVQSGGEQSVSAELILQKALDREKQAVIQLTLTALDGGKPAKSGTLQIVINVEDVNDNTPAFSKPLYKVRVAENSPLGSTAVKLEATDLDEGVNGELVYSISVGGSSKISDQFAIDTVTGEITVKGKLDHEENPALEIRAQARDKGTPSRVTHCKVLVEVLDVNDNAPEITVSSVMSPVKEDSTLGTVVALVTVSDKDSGKNGEAKAHVSDSVPFKLKPSYKNYYSLVVDGPLDRERTSQYNITITAADEGTPPLSSTSAIIVDVSDVNDNPPRFPEPVINVYVKENSQIGAVIHTESAFDPDVGENARVTYSLLESSKSSSVSSLVNINSDSGDIHSLQSFNYEEVKTFQFKVQATDSGVPPLSSNVSVNVFILDENDNSPGILAPYSEHGSVNTENIPYSAEAGYFVAKIRAVDADSGYNALLSYHISEPKGNNLFRIGSSSGEIRTKRRMSDNDLKTHPLVVVVSDSGEPSLSATVSVDVVVVESTGDIQTQFKHAPMKEESFSDLNLYLLIATVSVSVIFLLSLISLIAVKCCRTDGSFSRYSAPVITTHPDGSWSYSKSTQQYDVCFSSDTLKSDVVVFPAPFPPADAELINGVTSSLHLQIFCILRNSSSSGGRACTLKQLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUY1</accession>
    <name>A0A3B4DUY1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Membrane metallo-endopeptidase-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MMEL1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_017539128.1">
        <property type="nucleotide sequence ID" value="XM_017683639.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUY1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028202"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000578.2">
        <property type="protein sequence ID" value="ENSPNAP00000028202.1"/>
        <property type="gene ID" value="ENSPNAG00000002971.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411863"/>
    <dbReference type="CTD" id="79258"/>
    <dbReference type="GeneTree" id="ENSGT00940000157799"/>
    <dbReference type="OMA" id="DDAPNYG"/>
    <dbReference type="OrthoDB" id="6475849at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF141">
        <property type="entry name" value="MEMBRANE METALLO-ENDOPEPTIDASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="8">
        <location>
            <begin position="97"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="7">
        <location>
            <begin position="558"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="765" mass="88543" checksum="23E9A381D2DE86EB" modified="2018-12-05" version="1">MGKSESQMDIMEKTTKPRKHRWTVMEIGLSIMLLLMSCALAGLVVLYASALKEYSYRNGPSKGLQYHFSSESNVCTTPDCVTAAARLLQNMDPSVDPCQNFYQYACGGWLERHVIPETSSLHSVFNILRDELEIVLKGILEMEKQQDRLAFKKAKMLYRSCMNESVIEQRDSQPLLKLIDSIGDWPVASEDWNSTAEETWRLEDTLAALNAHYNKKILLEMFVWPDDRDSSRHIIHIDQPALGMPSRDYYLNDGNYRKVREAYLQFMVSMAKIAREDRNLTQDDERVWEEMMQVMKLEREIANATSPAEERNDITVLYNKMTLRDVQDTFSLDGFNWTRYIQGIMGSVSIAVQPEEPIVVYCSPYLEKLSHILSRYDRRTVQNYLVWMLAMERVSSLSRRFKDVRAHYRKALHGTTVEEARWRDCVRYVQSNMENAVGALYVQETFAGDSKRMVGELIRKIQEAFVETLEELNWMDDQSKEKAREKAMAISEQIGYPDYILEENNQKLDHEYAHLNFSEENYFENILENLQATARKGHKKLREPVDPDLWIIGAAVVNAFYSHNRNQIVFPAGILQPPFFSEKQLQALNFGGIGMVIGHEITHGFDDHGRNFDKHGNMYNWWSNYSTEHFEDQSKCMVQQYGTFSWKLAGGQNVSGISTLGENIADNGGVRQAYKAYMKWVEREGEEPRLPGLDLDHKQLFFLNFAQVWCGACRPEYAIQSIKTDPHSPLEYRVLGSLQNFEAFSEAFQCERGAPMNPDEKCRVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZD1</accession>
    <name>A0A3B4DZD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">HtrA serine peptidase 3a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565616.1">
        <property type="nucleotide sequence ID" value="XM_017710127.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZD1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028421"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014588.2">
        <property type="protein sequence ID" value="ENSPNAP00000028421.1"/>
        <property type="gene ID" value="ENSPNAG00000014134.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434764"/>
    <dbReference type="CTD" id="445108"/>
    <dbReference type="GeneTree" id="ENSGT00940000159570"/>
    <dbReference type="OMA" id="AHKGPCP"/>
    <dbReference type="OrthoDB" id="4217619at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06785">
        <property type="entry name" value="cpPDZ_HtrA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.120:FF:000002">
        <property type="entry name" value="HtrA serine peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22939">
        <property type="entry name" value="SERINE PROTEASE FAMILY S1C HTRA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22939:SF14">
        <property type="entry name" value="SERINE PROTEASE HTRA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017486111" description="HtrA serine peptidase 3a" evidence="9">
        <location>
            <begin position="21"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="11">
        <location>
            <begin position="23"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="12">
        <location>
            <begin position="73"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="377"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="52449" checksum="AC8BA82D0C0458AB" modified="2018-12-05" version="1">MRLLVSFSSFLLFLLSVVAGAEPRVRCSDVCDVSACPSPSCPAGYVPDRCGCCLVCAQGEGAPCGGAQQRRCGDGLECSGSAGKRVCQCKQSYRVCGSDGRTYSSPCRLRAASRKAQDRGQRALTQAHKGPCAPAKTGSTHPNSPRYKFNFIADVVEKIAPAVVHIELFVSHPLFGRHVPLSSGSGFIMTESGLIVTNAHVVTSSAAVTGHQQLRVQLHNGQTYEASIRDIDKKSDIATIKVNPKKKLPVLPMGRSADLRPGEFVVAIGSPFALQNTVTTGIVSTAQRDGKELGIQDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDRINRFLDESHDKHKVKHRLVRMQYMQSHPVVSDSDVTSSKKRFIGIRMMTLTEDLVEELKQQNPDFPDVSSGILVYDVIPDSPAQKGGLQTGDIIVKLNGRPLLSSDDLQFALQEDTPLLLEVHRGNDDLLFNIEPHIIMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZG5</accession>
    <name>A0A3B4DZG5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein FAM161A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">FAM161A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAM161 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZG5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028394"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000065.2">
        <property type="protein sequence ID" value="ENSPNAP00000028394.2"/>
        <property type="gene ID" value="ENSPNAG00000003027.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157824"/>
    <dbReference type="OMA" id="RENRWPY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051655">
        <property type="entry name" value="FAM161"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019579">
        <property type="entry name" value="FAM161A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21501">
        <property type="entry name" value="PROTEIN FAM-161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21501:SF3">
        <property type="entry name" value="PROTEIN FAM161A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10595">
        <property type="entry name" value="FAM161A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="67"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="290"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028394.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="440" mass="51396" checksum="7A4E1BA268D9223A" modified="2024-11-27" version="2">MLLPCNLRKAYSAHELRRDSTFLDTDEEDRAADNKRRDSSNLSIEKDLLVSPKERIKNMWQDFSVDRLSPRQRHPSATSIQSRADDRRAAVRNKVKVRGKRLKKRKQAGEEDGWRLRVTVPKPFRMTRREAERKQKGIKSRSEIEHENTELRRQLEELTECQRKFRASPMPAHVRLPLYEELQEKDEERRCHLRATEQQRLCSTQKPFSFLERERLKKELKEVQLKEQMLLSAKEEEDRRRRPFRAKPVPRAVKEAALGEQQKEEQLYRSIKMQMRARELLQSASMPPSMLARRLSERQAQKAATQSDQEAETSHRPKINGQVPDFDASYRRFQKQLASKRDVRPVTACEPFKLRTSNIASHKERIIADIEAERTSPRSSRWPFITPAALSPRTPSSSVSKITDAAKKRQEAVSNHINKPTLGQVLKSKLGKVLNLIQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZY4</accession>
    <name>A0A3B4DZY4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="9">MAD homolog</shortName>
            <shortName evidence="9">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037391376.1">
        <property type="nucleotide sequence ID" value="XM_037535479.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391377.1">
        <property type="nucleotide sequence ID" value="XM_037535480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZY4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029962"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035879.2">
        <property type="protein sequence ID" value="ENSPNAP00000029962.2"/>
        <property type="gene ID" value="ENSPNAG00000016416.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441801"/>
    <dbReference type="CTD" id="560317"/>
    <dbReference type="GeneTree" id="ENSGT00940000164585"/>
    <dbReference type="OMA" id="QPAFHTV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10492">
        <property type="entry name" value="MH1_SMAD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10498">
        <property type="entry name" value="MH2_SMAD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000002">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000002">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF68">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="12">
        <location>
            <begin position="14"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="13">
        <location>
            <begin position="342"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="163"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="243"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="271"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029962.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="571" mass="62382" checksum="B21CF4BB5BCEBBE6" modified="2024-11-27" version="2">MSVNPPNSNDACLSIVHSLMCHRQGGENEGFAKRAIESLVKKLKEKKDELDSLITAITTNGVHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKFCQYAFDLKYDNVCVNPYHYERVVSPGIVGLSLQNTGPSGRLIKEEYIHDCIQMDVPPGLPPQPDHQGAMKLPPPEHYSQPLPPLQLPPEPPRGPPPVTLYPNMPLSPTASGSMLPMQGGHSEGLLQIASPQAQVMTPTPPPSTPTHGPPQPPTPQQPPPNQNGYNGSKHTQSQGSFHTTWTGSSTASYTPVGPQQNSRGHQPPLHHPHFWSQHHSSPSFPPPVSNHPGPEFWCSISYFEMDVQVGEMFKVLSSCPVVTVDGYVDPSGGDRFCLGQLSNVHRTDASERARLHIGKGVQLECRGEGDVWMRCMSDHAVFVQSYYLDREAGRAPGDAVHKIYPGAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAVSLSAAAGIGVDDLRRLCILRLSFVKGWGPDYPRQSIKHTPCWVEVHLHRALQLLDEVLHTMPLADPGPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E092</accession>
    <name>A0A3B4E092_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NEK1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028726"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015161.2">
        <property type="protein sequence ID" value="ENSPNAP00000028726.2"/>
        <property type="gene ID" value="ENSPNAG00000003157.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158460"/>
    <dbReference type="OMA" id="ELNEEWH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08218">
        <property type="entry name" value="STKc_Nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000172">
        <property type="entry name" value="serine/threonine-protein kinase Nek1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="335"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="421"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="687"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="741"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="942"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1257"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="639"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="446"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="687"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="803"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="826"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="839"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="942"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1065"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1093"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1271"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1348"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1360"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1384"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1397"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028726.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1488" mass="164480" checksum="668C24311678CC09" modified="2024-11-27" version="2">MDKYEKVKKIGEGSFGKAILVRSREDGHQYVIKEIGISRMSSKERQESRREVAVLANMSHPNIVQYKESFEEGGCLYIVMDYCEGGDLFKKINNQKGVLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSQDLRSLLGLLFKRNPRERPSVCAILDKPFLARRIHKFLSPQLIAQEFSQSFLLKQHQMNAAQGVPAKRPAPGPAPITPSQKITKPAAKYGVPLTVKRPVCAGRKVADAAKKQVDRKPGVRPKQAPPPVAVQRRVSRVEEERRKHEEGVRKKQIELMEKERKQREQMLLLKAAQVKRFEREKLNRINRAREQGWRHVLSSSGGSSPERKFYGGGGMGAPPAPAPGPSPLPGPAHVPPPLPCPAPAPKEGPKASGDVSPVRAAGDPGLANGHACRTSKESVKKEPRRTSLQTETMSKNDYANRFRAQLAAERAKQVEEFLQRKREALLNKVRAEGQLGARQNLAAIFANRTSSSRCRRPRVNKEEEEYLARLRQIRLQNFNERQQIKARLRGEKYDSEYSDSQESSEEVELRKKKIEALKAQSKARAAVLKEQLEKKRREAYEREKRAWEDHLASRGVKVALGPPNAAAPSPAPSLLPETTPQDVVTKATPSCRPITPAISMTAALKDVGAIASLQNSPMKEEPQETETAAVHSERKEILRRLNQNLKAQTSKEEDEGEVAPTDVPGPIPQASQPVTEERPPTRNDRRRWEAGDPPILSVAQQTLEDTCSTMAASQAASPEGSAPSGADRRKWQPGAAPLSVAHQTLQDTSLTTAEQTTGEVIPMENWQGEGPRKAWGRTPVSEVLKILEEAELQPITQLLDNVSISDNTVTTVSAESLKTTTGPSDVTDGATLGPSPQPSHPAIGQDQESKAAIGQDQESKAAIGQDQESKAAIGQDQESKAAIGQDQESKAAIGQDQESKAAIGQDQESKAAIGQDQESKAAIGQDQESKAGIGQHSDLQSAFQESVSDAAALRSHEQQSGPAEKTLKSLRGEKESDGVDEVECLILKDGPQQTSESPAKVIHAWEQEVLHPGPDRGAVTQTEKPKDEERVDGEEEKQTEQAVSDEAAFVKLSCSPAHRRMVALSLLSAQSSMEDSSSSVASRSRSVSPLRTRRDDVLLIGLSTGLFDANNPKMLRTCSLPDLGKLFSASTEPDGVATHDNNLEMEDLEDKEDEQSETEDVYEDEDLRELRASMERLLQQQRSDDDDDEEGGSNGSPPDEERSVQCKASPTVEGLNGVTAEEEDGGDDDSADVSHEEEEGRLMSNGLNVADDEEQNSERHLNEEWHSDDSEEEEESDSEQQDSIFSRLEELRFHLEQAMGFDHFIQAYNKIKAIHEDEDENIDMGSSMVQSILGTEHQHLYPKILHLVMADGAYQEDNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0R6</accession>
    <name>A0A3B4E0R6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the dynein light chain family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017688205.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559877.1">
        <property type="nucleotide sequence ID" value="XM_017704388.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A3B4E0R6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016090"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015281.2">
        <property type="protein sequence ID" value="ENSPNAP00000028844.1"/>
        <property type="gene ID" value="ENSPNAG00000014609.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000037979.2">
        <property type="protein sequence ID" value="ENSPNAP00000016090.1"/>
        <property type="gene ID" value="ENSPNAG00000031659.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108415198"/>
    <dbReference type="GeneID" id="108431322"/>
    <dbReference type="CTD" id="373083"/>
    <dbReference type="CTD" id="572690"/>
    <dbReference type="GeneTree" id="ENSGT00390000000378"/>
    <dbReference type="OMA" id="MIEDTDM"/>
    <dbReference type="OrthoDB" id="10033309at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21452">
        <property type="entry name" value="DLC-like_DYNLL1_DYNLL2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF113">
        <property type="entry name" value="DYNEIN LIGHT CHAIN 2, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="89" mass="10364" checksum="42313D45C7707F8B" modified="2018-12-05" version="1">MTDRKAVIKNADMSEDMQQDAVDCATQAMEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0W8</accession>
    <name>A0A3B4E0W8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear receptor interacting protein 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017567401.2">
        <property type="nucleotide sequence ID" value="XM_017711912.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017567402.2">
        <property type="nucleotide sequence ID" value="XM_017711913.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0W8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028894"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015430.2">
        <property type="protein sequence ID" value="ENSPNAP00000028894.2"/>
        <property type="gene ID" value="ENSPNAG00000003221.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435820"/>
    <dbReference type="CTD" id="100001853"/>
    <dbReference type="GeneTree" id="ENSGT00390000007999"/>
    <dbReference type="OMA" id="SPPYACG"/>
    <dbReference type="OrthoDB" id="9878150at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026649">
        <property type="entry name" value="NRIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031405">
        <property type="entry name" value="NRIP1_RD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031406">
        <property type="entry name" value="NRIP1_RD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031408">
        <property type="entry name" value="NRIP1_RD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15088">
        <property type="entry name" value="NUCLEAR FACTOR RIP140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15088:SF0">
        <property type="entry name" value="NUCLEAR RECEPTOR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15687">
        <property type="entry name" value="NRIP1_repr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15688">
        <property type="entry name" value="NRIP1_repr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15690">
        <property type="entry name" value="NRIP1_repr_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="27"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear receptor-interacting protein 1 repression" evidence="3">
        <location>
            <begin position="409"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear receptor-interacting protein 1 repression" evidence="4">
        <location>
            <begin position="894"/>
            <end position="1060"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="867"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
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        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="345"/>
        </location>
    </feature>
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        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
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        <location>
            <begin position="570"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="872"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028894.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1098" mass="119866" checksum="F4C39ED1073734D3" modified="2024-11-27" version="2">MTHGEEPGPETHQDSAVLTYLEGLLMHPVASGPGATATRRSEAGNNDGEPGNQGTRGIQLPNHSSGPPEKGSPPGAASQHLKKARLLRSGAWSETDNRTKDAPRMEVNGQVNEHHKAALDSSAQGESTLLASLLQSFSSRLQTVAMSQCITQSHQQLDGESSESTPIDKDTFQGYCTASSRLKGLIRKNKQQSHNNVPYSRRNSQDRRSESPRNSQSSASDSMSCAERLKAVATLVKSRSSPAPSPKPSVACSQLALLLSSEAHLQQYSREQALKAQLSGRSASERLAAMATQQTMEKGPPSMGESLIAPDMLSSLHTLNGTLPSPTLNSSRRSPSLTPGRSISAGSPRPSPQPLKEKRPFDRHSSRPPQNCSSLLLLLLNNHNSQKHLTKNGHLDDDCTAPVSQASSHQSDSEYSNQENSLTKDSSDAESCYSSCSPIDLSMRSRICSQKSEPPSSASSLDKLTETLINKWKPETPGPKVPEARELETSPDMKSHHKVTLMQLLLDRKNNEKVNKIADNPDLQHDATLSNMAGGPLKRLGTLEEIRNQNALERLTGRGLSTFSHSRETSNTPSPFSYSSSLVQSSPLDLCKSKPHTNEKMLEPAFSASKLLQNLAHSGLQNPSPSPPSPQVHRPPSKRQSPECEPDRPQSLLDRLVPPIKRNKTPLFEPPPASFTLFGRERSPPASTQIENLLERRTVLQLLLGTASHKEKSSGRRSTEAAMGAFEKPPGASVICDSSNGPSLNIKIKTEPGEESLLSDSSDDGGCRWRSGHERYSPLAEAHDNVKAEECPTEAAAKYGLLSQLLKQQTTTYHSSPRTEPLVSTVKEEQKDYQEPLPKKRKLCLELAEHLSNELCTRPLDTVSERLVSGTPEEHKSRRLLSPKEEDPFIKSPAGKTPTRDSQGFNVLKQLLLSDNCLKELSQPRGTPSPFLPQANCKANGNFSQSTYNPELMNLPWFPQTLNSGTGNLKPLSSPPGNSTQGSPWAKGSPKPSPTPVKKESKSSPRWALGGEEYSPDSPPLTRSNPILYSMLQRGNGQLRKEMQDCVQRTHCGVRVKEEPCTESRYLEHRLSAMQHSLVHNERQSGGIEHLNGSLHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1S0</accession>
    <name>A0A3B4E1S0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1S0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029638"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016950.2">
        <property type="protein sequence ID" value="ENSPNAP00000029638.2"/>
        <property type="gene ID" value="ENSPNAG00000015878.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000214"/>
    <dbReference type="OMA" id="FLCCVLK"/>
    <dbReference type="OrthoDB" id="9986177at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048846">
        <property type="term" value="P:axon extension involved in axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002115">
        <property type="term" value="P:store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11722">
        <property type="entry name" value="SOAR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Stromal interaction molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Stromal interaction molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3550:FF:000001">
        <property type="entry name" value="Stromal interaction molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Stromal interaction molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand_STIM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032393">
        <property type="entry name" value="SOAR_STIM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037608">
        <property type="entry name" value="STIM1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15136:SF9">
        <property type="entry name" value="STROMAL INTERACTION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15136">
        <property type="entry name" value="STROMAL INTERACTION MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25578">
        <property type="entry name" value="EF-hand_STIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16533">
        <property type="entry name" value="SOAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043691534" description="SAM domain-containing protein" evidence="18">
        <location>
            <begin position="23"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="19">
        <location>
            <begin position="126"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="500"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="621"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="621"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="672"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="687"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029638.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="700" mass="78682" checksum="BF7BB884340CEE65" modified="2024-11-27" version="2">MEFSKLAALWIVCLCVLGECHTDKPNQVSTESLQVDNGMSDLCRIDEPLCQDENAILSFEAIRSIHKQMDDDANGNVDVVETDGFLREDLNYHDPKAKHNSFHGDDQFISVEDLWNSWKSSKVYNWTVDEVVEWLEAYVELPQYLEAFHKMNLNGTAMPRLAVKNSTLTVTVLKILDRSHTQKLQLKALDTVLFGAPMMNSHNHLKDFMLVVSIVIGMGGCWFAYIQNRYSKDHMKKMMKDLEGLQRAEQSLHDLQQKLRIAQEEHRTVEVEKVNLEQKLRDEINSAKQEAQRLRELREGTENELSRQKYAEEELEQVRMALKKAEKELESRCSWSPPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILAAKQALGEVTAALRERLHRWQQIEMLTGFTLVNNPGLPSLASALNLDPSFMGGRATPQHLAMSDDMDDMDEDIVAHGGLQCANRFMDRRASDLRSIGPDRQTLRKYSAPSMLSLRQRHIDPQLAMGSQRDLNRSDSDSSLSVSQTGEQLRLSYSSKGLPIKPTSLHHGPLSRSEESAQAQTPNGGNRVHDSGASPDGGPDSPILMKKIYGIEKSASMSEIMSMSESSRSLSPNSTDPDTPSPTGQPGIPGAKAGSRIPQLSLKKSPLEEDSGSTGEDTDSAGKKKHTFKIFKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1V0</accession>
    <name>A0A3B4E1V0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">snRNA-activating protein complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Small nuclear RNA-activating complex polypeptide 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SNAPC3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the SNAPc complex composed of 5 subunits: SNAPC1, SNAPC2, SNAPC3, SNAPC4 and SNAPC5. SNAPC3 interacts with SNAPC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAPC3/SRD2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037394203.1">
        <property type="nucleotide sequence ID" value="XM_037538306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1V0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029306"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016286.2">
        <property type="protein sequence ID" value="ENSPNAP00000029306.2"/>
        <property type="gene ID" value="ENSPNAG00000015340.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414754"/>
    <dbReference type="GeneTree" id="ENSGT00390000005708"/>
    <dbReference type="OMA" id="AHRDDCL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003681">
        <property type="term" value="F:bent DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022042">
        <property type="entry name" value="snRNA-activating_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13421">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13421:SF16">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12251">
        <property type="entry name" value="SNAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029306.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="380" mass="43785" checksum="41768CAB5BDEAA2C" modified="2024-11-27" version="2">MAEGGGGMENVPVYENVDVNTKPFHIGAFRDLWLEALKPEQYSFSRPEAAAEDVELMEEMGVSAETLEEVKTICSTESLRSHPADEVPDPDVVPPDSKLATLKLRKKKQDYKKTLVRESIGRHDIYANEMEMLAVGRKTDNPRNSVPEGEIILTFNIVYPVLFERFKYVRAYQTLHVLGSQKLTDLRDAICCVSDLQVFGEFSNTPDTVPQFISKDHYKSAFFFFEGTFFNDMRFPECRDISKTTRDWTKNKDFPDFKTAKMEDTLFYDLRVKVGYPYLYCHQGDCEHVVILTDVRLAHRDDCLDGTLYPLMTHKHRVVTRKCCVCHLYISRWITTNDTLAPMDPCLFCDQCFRQLHYDDDGNKLGDFQAYIYVDAGALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3D2</accession>
    <name>A0A3B4E3D2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3D2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018034.2">
        <property type="protein sequence ID" value="ENSPNAP00000030203.1"/>
        <property type="gene ID" value="ENSPNAG00000016787.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="OMA" id="WTLACMA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF966">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="48"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="334" mass="37851" checksum="5EDD1DF2D7384112" modified="2018-12-05" version="1">MTSMQLMSSTNSTFIRPSSFYITGLNNIPHAKYYYVFLCIVYAVTLMGNSFIMGTIYLARSLHTAKYVAVFNLALCDLCGSSALIPKLLDMFLFENQYISYEACLTNMFFVYVFMTLQSLTLLAMAYDRLIAICFPLRYHAIVTKTAMALIICVMWILSMSVNAILVALVTRLSFCRSTTVNSYFCDHGPVYKLACNDNSMNSIMVYVCMAALLYIPLFLIALSYVCISFALWRITQGVEQNKAMKTCTSHLILVTMFYLPIISVYTAALTTPLNANIRIINTALTQTIPPMLNPIIYTLKTEEVLQSIKVLYKRTKVNTMEQALKKRRTKVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4H4</accession>
    <name>A0A3B4E4H4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">SH3 and cysteine rich domain 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">STAC3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4H4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030655"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036278.2">
        <property type="protein sequence ID" value="ENSPNAP00000030655.1"/>
        <property type="gene ID" value="ENSPNAG00000017628.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183092"/>
    <dbReference type="OMA" id="NEEWWRI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20882">
        <property type="entry name" value="C1_Stac3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000022">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039688">
        <property type="entry name" value="STAC1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135:SF2">
        <property type="entry name" value="SH3 AND CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15135">
        <property type="entry name" value="STAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16664">
        <property type="entry name" value="STAC2_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="14">
        <location>
            <begin position="62"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="214"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="274"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="165"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="38218" checksum="3DEB8ECCC41CCDA0" modified="2018-12-05" version="1">MSQYDQLDDKDSVDIHDNPPAADNVVKEDNNTVYFIYDEEIEVEEKEEPPPPEPVKPVNDKPHKFKDHYCKKPKFCDVCARMIVLNNKFALRCKNCKTSIHHQCQSYVEFQRCFGKIPPGFRRAYSSPLYSSQQNPLMFSSQISQSNRTDPVFETLRIGVIMANKERKKGSEDKKQMMMMEEEESQPKAGEGAGEEGERDKMKRPQPGKLGVFSQSHYYLALYRFKAIEKDDLELHPGDRITVIDDSNEEWWRGKIGERSGFLPANYIIRVRSGERVYKVTRSFVGNREMGQITLKKDQIVVKKGDEVNGYLKVSTGRKLGFFPADILQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7H4</accession>
    <name>A0A3B4E7H4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PTPN1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7H4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031251"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020074.2">
        <property type="protein sequence ID" value="ENSPNAP00000031251.1"/>
        <property type="gene ID" value="ENSPNAG00000018498.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158041"/>
    <dbReference type="OMA" id="EFWEIDE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070373">
        <property type="term" value="P:negative regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903898">
        <property type="term" value="P:negative regulation of PERK-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000025">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051985">
        <property type="entry name" value="NR_tyrosine_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012265">
        <property type="entry name" value="Ptpn1/Ptpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46047:SF2">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46047">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 61F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000926">
        <property type="entry name" value="Tyr-Ptase_nr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="187"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="384"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="10">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="213"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000926-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000926-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="476" mass="54043" checksum="C4DA52D513565E73" modified="2018-12-05" version="1">MEAEFREIDESGNWNAIYQEIRQQSSELPCRIAKLPENRSRNRYRDVSPFDHSRIHLQLGNNDYINASLISMEEAQRSYILTQGPLPNTCGQFWEMVWEQRTRGVVMLNRVIEKGSVKCAQYWPQKEEREAFFEDTNFKLTLVSEDVKSYYTVRQLELENLSTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGCLNSDQGPVVVHCSAGIGRSGTFCLVDTCLLLMSLRKDPSSVQIKDVLLDMRQYRMGLIQTADQLRFSYLAVIEGAKSIMGDTSVQESWKELSNEEDPPPEFTPPPRPRLLKDPHNGMGDPVKFEPTPRFFPEHELIQERNEVHIHSAPPETALRQRTVAVGAGETSTETLPNDQLDHANALKPSYTVPETTEPPSKPARSLPETHTAPPGGTAAGEWSPLLANVCVCTALILAATRGKVRIRKALWPHAAGVMEWTRDKRDKHSHLHQKAGMRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E916</accession>
    <name>A0A3B4E916_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Tyrosine aminotransferase</fullName>
            <shortName evidence="13">TAT</shortName>
            <ecNumber evidence="5 13">2.6.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TAT</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-tyrosine + 2-oxoglutarate = 3-(4-hydroxyphenyl)pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15093"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="2.6.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.5" evidence="5 13"/>
    <dbReference type="RefSeq" id="XP_017538260.1">
        <property type="nucleotide sequence ID" value="XM_017682771.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E916"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033067"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037707.2">
        <property type="protein sequence ID" value="ENSPNAP00000033067.1"/>
        <property type="gene ID" value="ENSPNAG00000021482.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411257"/>
    <dbReference type="CTD" id="6898"/>
    <dbReference type="GeneTree" id="ENSGT00940000156704"/>
    <dbReference type="OMA" id="CALDLCI"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00338"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000048">
        <property type="entry name" value="tyrosine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000040">
        <property type="entry name" value="Tyrosine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005958">
        <property type="entry name" value="TyrNic_aminoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005957">
        <property type="entry name" value="Tyrosine_aminoTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01264">
        <property type="entry name" value="tyr_amTase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01265">
        <property type="entry name" value="tyr_nico_aTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF2">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000517">
        <property type="entry name" value="Tyr_transaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="10 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="15">
        <location>
            <begin position="116"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="14">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000517-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="498" mass="54648" checksum="92398F3919F5E3E1" modified="2018-12-05" version="1">MSYVIRMNGNSNGIQKDALNGKGIGRNGISENGIDRNCVSKNGVNGNSFSVNGFDGNGFKGISVHHVSARVPVSAAKLKARRQRWNVRASEMSKNTLNPIRAIVDGMKLTPNPEKPMIALSIGDPTVFGNLPTDDSVLQAMKDAIDSHKFNGYAPSVGYLQSREAVANFYSQAGAPLEAKDVILTSGCSQAIELAISALCNPGDNILVPCPGFSLYKTLAVSVGVQVKFYNLLPEKSWEVDLQHLESLIDDRTTCLIVNNPSNPCGSVFTKEHLRNILAVASRHCIPILADEIYGDMVFPSCEFHSLANLSSDVPILSCGGLAKRWLVPGWRMGWILIHDRNNIFGSEIREALVKLSQRILGACTVVQGALESILNNTQAEFYQSTIGFLKSNSEICFAELSTVPGLNPVMPSGAMYLMVGIEMEHFPEFQNDVDFTERLVTEQSVFCLPATAFEYPNFFRIVVTVPEEMMVEACGRIREFCARHYRPLSQESNDLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EBD9</accession>
    <name>A0A3B4EBD9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-ESSS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase ESSS subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NDUFB11</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Complex I is composed of 45 different subunits. Interacts with BCAP31.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB11 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538782.1">
        <property type="nucleotide sequence ID" value="XM_017683293.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EBD9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033013"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023489.2">
        <property type="protein sequence ID" value="ENSPNAP00000033013.1"/>
        <property type="gene ID" value="ENSPNAG00000005364.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411636"/>
    <dbReference type="CTD" id="54539"/>
    <dbReference type="GeneTree" id="ENSGT00390000003022"/>
    <dbReference type="OMA" id="GSVFVHY"/>
    <dbReference type="OrthoDB" id="5917019at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001944">
        <property type="term" value="P:vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019329">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ESSS_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13327:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 11, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13327">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE ESSS SUBUNIT, MITOCHONDRIAL PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10183">
        <property type="entry name" value="ESSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="139" mass="15785" checksum="F726994AF9B9016B" modified="2018-12-05" version="1">MAPLLRALRAGLRSVCLARAVSNSARSRGAVVTELQPRAADREEHGEVNQFQHNPDYHGFSSDPFVDEWNMRLAFFFSISMAIVIGGTFIHYLPDHGMRQWARREAELLIKQREAQGLPLIDENYYDPSRIVLPSSSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EC53</accession>
    <name>A0A3B4EC53_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EC53"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032884"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037597.2">
        <property type="protein sequence ID" value="ENSPNAP00000032884.2"/>
        <property type="gene ID" value="ENSPNAG00000021154.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166366"/>
    <dbReference type="OMA" id="IGNQREF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22032">
        <property type="entry name" value="HMG-box_SoxF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF11">
        <property type="entry name" value="CASANOVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="42"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="42"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032884.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="276" mass="31438" checksum="AB87B1A28A15F6D7" modified="2024-11-27" version="2">FPSCLVVASCSRSDYLEPFPQSPQSAESELSCSSPEGKAPRVRRPLNAFIIWTKEERRRLAQLNPELENTDLSKILGRTWKAMSLAEKRPYMQEAERLRVQHTIDHPNYKYRPRRRKSNRRSSKTPPSDTSTSSNIHLSCMLHNQALHYPYTHSHTLSNTYTHPHSLAFPTHQTAAQNGVGFPNGGVSFSEGAFLSSLAYPQQAMCSAQPQYYSSQHVVQQRVEHWRGTEVCGCVLCSGGPSLEFYLEQVRVDMLDQLDRSEFDQYLNPVPTKDQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EDJ8</accession>
    <name>A0A3B4EDJ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PRKRA</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRKRA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EDJ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033369"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024190.2">
        <property type="protein sequence ID" value="ENSPNAP00000033369.2"/>
        <property type="gene ID" value="ENSPNAG00000021962.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157618"/>
    <dbReference type="OMA" id="PEYEFEK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070920">
        <property type="term" value="P:regulation of regulatory ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19863">
        <property type="entry name" value="DSRM_PRKRA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19892">
        <property type="entry name" value="DSRM_PRKRA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000007">
        <property type="entry name" value="Double-stranded RNA-binding protein Staufen homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000005">
        <property type="entry name" value="Putative double-stranded RNA-specific adenosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000019">
        <property type="entry name" value="RISC-loading complex subunit TARBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044467">
        <property type="entry name" value="PRKRA_DSRM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051247">
        <property type="entry name" value="RLC_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032478">
        <property type="entry name" value="Staufen_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205:SF2">
        <property type="entry name" value="INTERFERON-INDUCIBLE DOUBLE-STRANDED RNA-DEPENDENT PROTEIN KINASE ACTIVATOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46205">
        <property type="entry name" value="LOQUACIOUS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16482">
        <property type="entry name" value="Staufen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="18"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="110"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="220"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033369.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="293" mass="32056" checksum="5F1843DF8CBCD2B0" modified="2024-11-27" version="2">MTESHSAVAGQHVAADKTPIQVLHEYGAKMGRPPVYVMERADGEAHQPLFVFSVTVGDVTCSGQGPSKKAAKHEAAEAVLKLLELDAENSPQNPSLENNGYGAETNDQPNPVGILQELAMQRAWRLPEYVVCMESGPAHKKEFTVTCRLETLSETGTGSSKKNAKRVAAEKMLEKLQSLSGSPEITWSPKPHVYLENLRSSKLEKISLLRRNPLSIPNTDYVQMMLELSQEQGFEVTYFDIDELTVNGQYQCLAELSTTPVTVCHGTGISCSNAHNDAAHSALQYIKIMATSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFD6</accession>
    <name>A0A3B4EFD6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphodiesterase</fullName>
            <ecNumber evidence="15">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PDE6A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="15">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017545563.1">
        <property type="nucleotide sequence ID" value="XM_017690074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFD6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034555"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026501.2">
        <property type="protein sequence ID" value="ENSPNAP00000034555.1"/>
        <property type="gene ID" value="ENSPNAG00000024013.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108417948"/>
    <dbReference type="CTD" id="5145"/>
    <dbReference type="GeneTree" id="ENSGT00940000156471"/>
    <dbReference type="OMA" id="GPHFTKR"/>
    <dbReference type="OrthoDB" id="546632at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000005">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000010">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0140">cGMP</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0844">Vision</keyword>
    <feature type="domain" description="PDEase" evidence="17">
        <location>
            <begin position="484"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="833"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="835"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="560"/>
            <end position="564"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="600"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="721"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="721"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="858" mass="99107" checksum="9194E5C8DDE473A9" modified="2018-12-05" version="1">MAVVNQDVAEKFLDSNPAFAKQYYDTHFRAEVISNLLSTSKKAEVNFNQYHNLSSLEESEVVFDMVRDMQENLQMERAVFNLMRHLCFITRADRMSLFMYRQRNGTAELATRIFDVNKDSTLEDCLVPPECEIVYPLDTGIVGHVASVKKIVNVPDVSQSPFFSDFVDTLTEYTTKNVLATPIMNGKDVVAVIMAVNKIDGPCFTAQDEQTLQKYLNFANLVLRVFHLSYLHNCETRRGQVLLWSASKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKTKEFFDLWPILMGEVPAYSGPKTPDGREINFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPSYVAETGLICNIMNAAEDDFFEFQKEPLDGTGWTIKNVLSLPIVNKKEEIVGVATFYNRKDGKPFDEMDETLMESLTQFLGWSVLNPDTYERMNKLENRKDIFQDMVLYHVKCRKDEIQNILNTRELYGKEPDECEEDELEEILVEVLPDSEQSEIFEFHFCDFEHSELDLVKCGILMYYELGVVDKFHIPREALVRFMYSLSKGYRKITYHNWRHGFNVGQTMFTLLMTGDLKRYYTDLEAMAMLTAGFCHDIDHRGTNNLYQMKSGNPLAKLHGSSILERHHLEFGKTLLRDDALNIYQNLNRRQHDIVIRLMDIAIIATDLALYFKKRAMFQKIVDQSKTYENWDDWTKYMMLESTRKEIVMAMMMTACDLSAIAKPWEIQSKVALSVAAEFWEQGDLERTVLEQQPIPMMDRNKADELPKLQCGFIDFVCSFVYKEFSRFHVEITPMLERLLNNRKEWNALKEIHEAKIAKIEEAKKAKEEAAAAATAAKQGNAAQASQSKTCVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EN08</accession>
    <name>A0A3B4EN08_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EN08"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036659"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040190.2">
        <property type="protein sequence ID" value="ENSPNAP00000036659.2"/>
        <property type="gene ID" value="ENSPNAG00000027673.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272581"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036659.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="133" mass="15091" checksum="AB8F9A15821DA970" modified="2024-11-27" version="2">MQNSEPLVISAEPGEAVTITCTFVDDSSADVRMWYKQRLEHVPLEVGSKIKDKDPVMSPHFKQTRFKIDRIANAISLRIEPVTKEDEGMYFCGLGRQNAMDFSTATFLAVTGDILIYCSQCCFYVKCLFNKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IHA5</accession>
    <name>A0AAR2IHA5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA 2-O ribose methyltransferase substrate binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IHA5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053625.1">
        <property type="protein sequence ID" value="ENSPNAP00000038615.1"/>
        <property type="gene ID" value="ENSPNAG00000019511.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017317"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18106">
        <property type="entry name" value="SpoU-like_RNMTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053888">
        <property type="entry name" value="MRM3-like_sub_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051259">
        <property type="entry name" value="rRNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43191">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43191:SF2">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22435">
        <property type="entry name" value="MRM3-like_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="6">
        <location>
            <begin position="154"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="358"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="477" mass="52378" checksum="3888F2ADDE2729C7" modified="2024-11-27" version="1">MAALFGGVGREITLFMGRGSCLRGWNSVVVTSRRFIRGLRRRPVAVLHPGGERETRVKSNETADQPRLTRGDAGGTGWERSRRRRAEDETFHAGAAARNPGHGSRPAAGASCSGVREQLGGLRFEKAQPGDKRLPKVVSIARSRAFREQQGKVLLEGRRLICDALAAGAAPQTLFFSRVERLCELPLDKLRQASLVKVKFEDIKIWSDLVTPQGVLAIFSKPDASRLSFPKDLRLQSVPLFLICDNVRDPGNLGTILRCAAAVGCDRVLLNKGCVDAWEPKVLRAAMGAHFRLPIFPGLDWNDISQHLPKPVTVHVADNVHKLMVRQDAEAAPQDAEKPGDVGWVCNSDSSRNKHLEEYVESDSDANSDSDDDSDSDDELSRPYLEPKAYHENWAQRSTALVISGEIHGLSSEALQLAEETGGHRLSVPMAPGVESLNSAMTAGILLVEGRRQLMNFTQKAIPRNQCVLHQFQGQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IK68</accession>
    <name>A0AAR2IK68_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Guanylate cyclase</fullName>
            <ecNumber evidence="3 14">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Photoreceptor outer segment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 14"/>
    <dbReference type="RefSeq" id="XP_017556634.1">
        <property type="nucleotide sequence ID" value="XM_017701145.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IK68"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075647.1">
        <property type="protein sequence ID" value="ENSPNAP00000038422.1"/>
        <property type="gene ID" value="ENSPNAG00000025795.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429428"/>
    <dbReference type="GeneTree" id="ENSGT00940000165991"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06371">
        <property type="entry name" value="PBP1_sensory_GC_DEF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14043">
        <property type="entry name" value="PK_GC-2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000404">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000013">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000114">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF349">
        <property type="entry name" value="RETINAL GUANYLYL CYCLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12 14" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="522"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="869"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="806"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1106" mass="124356" checksum="F12C250E90A050F3" modified="2024-11-27" version="1">MLTPTVKEPRIPCLPHGAWLLSFFLAIFSVPYIVQAAVFRVGVLGPWSCDPMFAKAMPGVAAQLAVQRINRDHSLSVGITFDYVILEEDCQTSMALRGFMGYYTQAAAFLGPINPGYCEAASLIAKNWNKALFSWSCVNHELESSWRHPTFVRTLTSPIWVLRILARHFHWANIAIVASADDVWMDTSNKVADALRSHGFPVRMVLSTSNDPSSIRHSLSKIKKLKELRMVILCMHSALIGGVTQKLLLETALDMHLTDGSVVFVSYDTLLYSLPYKQVLHPALRRNSKLRLAYDAVLTVTMDSEENSFYKAYQDAIKSKELPASIQPHEVSPLFGTIYTSIIFMANTLQNIHKSTNMFSGANVARHTGNLAFQGFSQKVRTNGSGVSLLNYLVLDTNGQSWELHPTYSVDVEGNMVRYLGVPIHFPGGAQPKADSSCWFTPGGPLCTRGVDPLLLFEVFMSALLVIVLATSLGYCVRRRISKIQMVSGPNKILLTLDDVTFINPSLSHKKVSLEDSKAELERRLSDMDSGMNSPLSTSSATEPSYENSNVAIFEGDWVWLKRLPYGHFGSINPKTSDVFELMRDIRHENLNPFMGFFHDCGVFAIVTEFCSRGSLEDLLRNEDVKLDWMFKSSLLLDLIKGMKYLHHRGVFHGRLKSRNCVVDGRFVLKVTDYGYNEVLQAQKFPYTDPKAEDLLWTAPELLRTAHPGLSGTHEGDVYSFSIVMQEVVLRGPPFFMLHMPAEEIIQRIKKPPPLCRPVVSPDYAPMECIQLIKQCWNEQADRRPSFDEIFEQFRNINKGKKTNIIDSMLRMLEQYSSNLEELIRERTEELEIEKQKTEKLLTQMLPPSVAEALKVGCTVEPEYFDNVTLYFSDIVGFTTISANSEPIEVVDLLNDLYTLFDAIIGNHDVYKVETIGDAYMVASGVPVRNGNRHAVEIANMALDILSAVGTFKMRHMPDVPVRIRIGLHTGSCVAGVVGLTMPRYCLFGDTVNTASRMESTGMPYRIHVSHTTVNILLDLKEGYKVELRGRTELKGKGMEETYWLVGRDGFTKPLPVPPELKSGPETARDFKQVLQKAVRRLSFVRHVAQLPTMESTDDNVMIHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IPI3</accession>
    <name>A0AAR2IPI3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LPAR1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IPI3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087706.1">
        <property type="protein sequence ID" value="ENSPNAP00000041610.1"/>
        <property type="gene ID" value="ENSPNAG00000027815.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271896"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070915">
        <property type="term" value="F:lysophosphatidic acid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15101">
        <property type="entry name" value="7tmA_LPAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000025">
        <property type="entry name" value="Lysophosphatidic acid receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004065">
        <property type="entry name" value="LPA_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002277">
        <property type="entry name" value="LPA_rcpt_EDG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01148">
        <property type="entry name" value="EDG2RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01527">
        <property type="entry name" value="LPARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
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    <sequence length="361" mass="41291" checksum="30A62106C3C17F0F" modified="2024-11-27" version="1">RATWLNSIPVHPQSFNMEEQCYYNETIAFFYNKSGKYLATEWNTVSKLVMGLGITVCIFIMLANLLVMVAIYINRRFHFPIYYLMANLAAADFFAGLAYFYLMFNTGPNTRRLTVSTWLLRQGLIDTSLTASVANLLAIAIERHITVFRMQLHTRMSNRRVVVVIMIIWTMSIIMGAIPSVGWNCICAIETCSSMAPLYSNSYLVFWAIFNLVTFVVMVVLYAHIFMYVRQRTMRMSRHSSGQRRNRDTMMSLLKTVVIVLGAFIVCWTPGLVLLLLDVFCNDCNVLAYEKFLLLLAEFNSAMNPIIYSYRDKEMSVTFKQILCCQRQENVNGTAEGSDRSASSFNHTVLSSPHNNDHSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITJ8</accession>
    <name>A0AAR2ITJ8_PYGNA</name>
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        <recommendedName>
            <fullName evidence="14">Neural cell adhesion molecule 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NCAM2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="13">May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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        <property type="component" value="Chromosome 6"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="715"/>
            <end position="742"/>
        </location>
    </feature>
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            <begin position="17"/>
            <end position="125"/>
        </location>
    </feature>
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            <begin position="132"/>
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    </feature>
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            <begin position="511"/>
            <end position="609"/>
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            <begin position="611"/>
            <end position="706"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="866" mass="96515" checksum="20F99C6FE1C7DB18" modified="2024-11-27" version="1">MGSGSQICHHPQDWLLPDCTIINHFAKADDFLATHCVLQVSISLKKVELSVGESKFFTCTAIGEPVSLEWYNPQGERMVPSSRVALHNEGVRSRLTLYNANVEDAGIYRCQATDAQGQTQEATVVLEIYQKLTFRDVPSPQEFRQGDVAEVVCDVTSSPVPVVAWYYNNIEITEDTEKFQLLPNNNLQVQRVTKADEGIYRCEARVEARGEIDFRDIILVVNVPPVLSVPQLSFNATADYGESVTFTCRAYGSPEPDVTWHRKGVELQESEQYVMRARGTTLTIRNIKQDDGGSYTCKASNKAGEMQQEIFLKVFVQPHITKLRNVTAVEGSAAMISCKAEGEPLPEISWRRASDGHSFSDGDKSPDGRVEVRGRHGESMLTIVGVGLSDWGRFDCEALSRIGGHQKSMFLDIEYAPKFQTNHTIFFSWEGNPVNISCDVMSNPPATMLWRREKLTIPTEGTANMRVHNAPGRSLLEVTPMSDRDFGRYNCTARNNIGMRFQEFILAQADVPSNPYSVRLSSVAQRTATVTFMKPDSHGGVPIDHYLVRYKDISSHDWRDVKSHGTQSESVVLLTNLEPNTTYEVRVAAVNGKGQGEFSRTESFQTLPIREPSPPSVQGQRGVGKAYRLGLVKQDDGGMPILEYIIKYKTDKEEQWMTKLVPGMNDFVLLQPLQWNTQYEVEITARNVKGLSEPTFVKFSMPQKPDITDSLFSELGLGAVVGLGLAGLLLLLVLVDVSCFFLRQCGLLMCITRKLCSKKTATSGKSKELEEGKAAYLLCMYGEDSWGRALSYSKYVTFSGGALSGFFMSSTEEASVFQSCNMAVRRVPWLRGIKSCHVYLALSSEGLEGKIAFSSNNKQHGFKRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWE0</accession>
    <name>A0AAR2IWE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cleavage and polyadenylation specificity factor subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CPSF1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPSF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037391380.1">
        <property type="nucleotide sequence ID" value="XM_037535483.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000060889.1">
        <property type="protein sequence ID" value="ENSPNAP00000042367.1"/>
        <property type="gene ID" value="ENSPNAG00000024395.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438863"/>
    <dbReference type="GeneTree" id="ENSGT00950000183151"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.910:FF:000005">
        <property type="entry name" value="Cleavage and polyadenylation specific factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002223">
        <property type="entry name" value="Cleavage and polyadenylation specific factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000100">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000118">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000544">
        <property type="entry name" value="cleavage and polyadenylation specificity factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="7">
        <location>
            <begin position="17"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="8">
        <location>
            <begin position="526"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="6">
        <location>
            <begin position="1086"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="543"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="756"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="760"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1456" mass="164010" checksum="763FADBB4B325CBB" modified="2024-11-27" version="1">MYAVYRQAHPPTGLEFSVYCNFISSEEKNLVVAGTSQLYVYRIIHDFESSLKTEKPSDGKSRKEKLEQVASFSLFGNVMSMASVQLVGTNRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHFFEEPELRDGFVQNVHIPIVRVDPENRCAVMLVYGTQLVVLPFRKDTLTDEQEGIVGEGQKSSFLPSYIIDVRELDEKLLNIIDMKFLHGYYEPTLLILYEPNQTWPGRVAVRQDTCSIVAISLNIMQKVHPVIWSLSNLPFDCTQVMAVPKPIGGVVVFAVNSLLYLNQSVPPFGVSLNTQTNGTTAFPLRVQEEVKLTLDCSQAAFITSDKMVISLKGGEIYVLTLITDGMRSVRAFHFDKAAASVLTTCMVTMEPGYMFLGSRLGNSLLLKYTEKLQETPMEEGKDKEKEKEKEKQEEPPNKKKRVDSTANWAAGKASMPDELDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCVSASMGEPAFLSEEFQSNPEPDLEVVVCSGYGKNGALSVLQRSIRPQVVTTFELPGCHDMWTVVSSEEKPEKKPPAEGEGEDAEEEKQEPTVEDEKNKHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVYAGNIGENKYIIQVSPMGIRLLEGVTQVHFIPVDLGSPIVQCSVADPYVVIMTAEGVVTMFVLKSDTYMGKSHRLALQKPQLHTQSRVITLSAYRDVSGMFTTENKINFLVKEETMARSQTETETVIQDISNTVDDEEEMLYGDSNPLNSPSKNETTRGTPAASASVQLERPGGQGKQEPTHWCMLVRENGVMEIYQLPDWRLVFLVKNFPVGQRVLVDSSAGQSAAQGETKKEEVTRQGEIPLVKEVTLVSLGYNHSRPYLLAHVDQELLIYEAFPYDQQQAQSNLKVRFKKMPHNINFREKKSKLKKDKKSEALGEDSLGAKGRVARFRYFEDISGYSGVFICGPSPHWMFVTSRGAMRLHPMTIDGSIESFSPFHNVNCPKGFLYFNKQGELRISVLPTYLSYDAPWPVRKIPLRCTVHHVTYHVESKVYAVCSSIKEPCTRIPRMTGEEKEFEVIERDERYIFPQQEKFSIQLISPVSWEAIPNTRIDLEEWEHVTCMKTVALKSQETVSGLKGYVAVATCLMQGEEVTCRGRILILDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGYLVSAIGQKIFLWSLKDNDLTGMAFIDTQLYIHQMYSIKNFILAADVMKSISLLRYQEESKTLSLVSRDARPLEVYSIEFMVDNNQLGFLVSDRDKNLMVYMYLPEAKESFGGMRLLRRADFNVGTHVNTFWRMPCRGILDPSSKKALTWDNKHITWFATLDGGVGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPKAFRMLHTDRRTLQNAVRNILDGELLNKYLYLSTMERSELAKKIGTTSDIILEDLLEIDRVTAHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IX31</accession>
    <name>A0AAR2IX31_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540798.1">
        <property type="nucleotide sequence ID" value="XM_017685309.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IX31"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066041.1">
        <property type="protein sequence ID" value="ENSPNAP00000042607.1"/>
        <property type="gene ID" value="ENSPNAG00000002681.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413014"/>
    <dbReference type="CTD" id="83775"/>
    <dbReference type="GeneTree" id="ENSGT00940000165883"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000001">
        <property type="entry name" value="Cryptochrome 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF63">
        <property type="entry name" value="PHOTOLYASE_CRYPTOCHROME ALPHA_BETA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="8">
        <location>
            <begin position="3"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="507"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="249"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="290"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="388"/>
            <end position="390"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="551" mass="63389" checksum="DBDE622E75748149" modified="2024-11-27" version="1">MPHRTLHLFRKGLRLHDNPTLLGALESSSVVFLVYVLDRAFEEEAMRVGFLQWRFLLQSLLDLRGRLSAVGSRLYVLQGSHRAVLRELVVRWRITQISFDLETEPHHVALQRELGEMAQELGLTVHTCVAHTLYDPRRIIDANGGEAPLTYKKFLRVVSVIGEPDKPVRDITAEDFQRCYSPEEVECEQHCIPSLSELGLDGSDEAQAEVLWPGGETHALQRLHKHLESQGWVQNSKPQAVPNSLWPNTTGLSPYFSFGCLSVRTFYHTLSRVYARARNHALPPVALQGQVLWREFFYTAAAHTPNFTRMEGNPICLQIRWNHDPDALQKWRTAQTGFPWIDAIMTQLRQEGWIHHLSRHAVACFLTRGDLWISWEEGMKVFEEYLLDADYSVNAGNWLWLSASAFFHQYNRIFCPVRFGRRTDPEGRYIRKYVPVLKNFPSRFIYEPWSAPEEVQLQAGCIIGEDYPFPMVCHAEASERNLALMKEARSCQENTAKLTRDVAEEPVDVRVKRELGEEDQPSKRFSSDPQSRPCSWGQENPRLPEITGQAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IY56</accession>
    <name>A0AAR2IY56_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IY56"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083379.1">
        <property type="protein sequence ID" value="ENSPNAP00000044678.1"/>
        <property type="gene ID" value="ENSPNAG00000030586.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282554"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="94"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="568" mass="63568" checksum="C679BBF30FD4FAB1" modified="2024-11-27" version="1">MDFFNNKIDNIRQQIQPTVSNSNPALLPSDLADIEQNTAVEKSLETFHPLPQLELEKMISSANCTTCTLDAIPSKLLKEVLPVIINPISTIVNSSLSLGHVPKAFKLAVIKPLIKKPNLDVAVLSNYRPVSNLPFISKILEKAVAQQLSSYLHKNHIYEKFQSGFRPHHSTETALVKITNDLLLASDQGYISLLVLLDLSAAFDTIDHNILLERLENMVGVTGTALSWFRSYLSERYQFVRVNNLSSNYSKVRFGIPQGSILGPLLFTLYMLPLGTVISNHGVNFHCYADDTQLYISAKPDDQHRLKKMEDCVKDVKGWMSRNFLLLNSNKTEVLLLGPKLARSKSPDLILNLADFPATPGSAAKNLGVIIDSDLSFDQHIGSITRTAFLHLRNIAKIRNALSLRDAETLVHAFITSRLDYCNALLSGCTSRNLNKLQLVQNAAARVLTKTRKFEHISPALSSLHWLPIKFRIDYKILLLTYKALHGLAPEYLQDLISHYEPSRPLRSQSAGLLIVPRIQKVSAGGRAFSYKAPKLWNDLPETVRDSDTVSIFKTRLKTHLFSLAFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYE6</accession>
    <name>A0AAR2IYE6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Lysophospholipid acyltransferase 5</fullName>
            <ecNumber evidence="15">2.3.1.23</ecNumber>
            <ecNumber evidence="16">2.3.1.n6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LPCAT3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.23" evidence="15"/>
    <dbReference type="EC" id="2.3.1.n6" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYE6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057530.1">
        <property type="protein sequence ID" value="ENSPNAP00000042626.1"/>
        <property type="gene ID" value="ENSPNAG00000019667.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234564"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071617">
        <property type="term" value="F:lysophospholipid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF14">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="468" mass="53794" checksum="85E2F8A7C70F4517" modified="2024-11-27" version="1">MRLNWEAQLGLLVRIRRSTLNGAAVTFWRLRRYPFALVYRRFLFHQSASVIHLFHTLSGLSLAIFNFGSQVYHSALCIIVQFLFLRLMGRTVTGVLSSFIFQMAYLLTGYYYTATDEYDIKWTMPHCVLTLKLIGLSLDYYDGGKDPSQLSAEQKQGALSSTPSLLEVCGFSYFYGGFLVGPQFTLRNYQSLVSREMSDCPGQVPNSAIPALKRFSLGLVFLAIFAVCGPYYPDSYYLTDEFEAQPFWYRCVFILLWAKVNLYKYVSCWLITEGVCILTGLGYNGRSKTGEPQWDACANMRVWMFETTPLFTGTINSFNINTNAWVARHIFKRLKFLGNKMVSQMATLLFLAIWHGLHSGYILCFTMEFIIVNVERQAQALVKDSPTLTAITNGPFYPLIYVVQQFIHWLFMGYPLVPFCLFTYDKWLKVYSSVYFCGHLLFFSAVLILPYLRKALVPRKAKSDKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J3I2</accession>
    <name>A0AAR2J3I2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Refilin B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RFLNB</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with FLNA and FLNB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Refilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J3I2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070774.1">
        <property type="protein sequence ID" value="ENSPNAP00000046583.1"/>
        <property type="gene ID" value="ENSPNAG00000009161.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016836"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031005">
        <property type="term" value="F:filamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061572">
        <property type="term" value="P:actin filament bundle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900158">
        <property type="term" value="P:negative regulation of bone mineralization involved in bone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061182">
        <property type="term" value="P:negative regulation of chondrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048705">
        <property type="term" value="P:skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028215">
        <property type="entry name" value="Refilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31848">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31848:SF2">
        <property type="entry name" value="REFILIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15068">
        <property type="entry name" value="FAM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25802" checksum="52A57BD835B40D90" modified="2024-11-27" version="1">MVGRLNLRNVSDDDLLDMNFKAERALDSPDSGLPPSPSPSPNPWLLAAGSERGSGGSEDEGRVRNAEVKSVAETAACPRCGQSLRVLLLSNFCYSLHPCSYGEGIELDPLPLKELRYTSSVRYDSERHFIQDVSLQPTGLGLELCSQTVLALSQSTWRRYKTQLEFQPRQRVQRYQSTTIVYPKHARTLYTTQLNYDGRNLAKRFLSSVELEASDYHSLTRSLCILCVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J3Y7</accession>
    <name>A0AAR2J3Y7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TAAR1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J3Y7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081175.1">
        <property type="protein sequence ID" value="ENSPNAP00000044521.1"/>
        <property type="gene ID" value="ENSPNAG00000036602.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244823"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15055">
        <property type="entry name" value="7tmA_TAARs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000279">
        <property type="entry name" value="Trace amine-associated receptor 16f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="331" mass="37209" checksum="061A553887B0729F" modified="2024-11-27" version="1">MSIQYCFPGVNSSCRKEVQTGPAYIFLFIVLSCVSVFTVFLNLLVIISISHFKQLHIPTNLLILSLAVADLLVGLFVMPVKIMELIDSCWYLGKIACSVSPIIYNLSMSASLCSLVFIAVDRYIAVSDPLLYSTRVTVCKISVFIIIGWSFCSFYVIIFLYTNDHLLLSQISSECYGKCVLFVKYSWVIIDMVIFFLAPCSVILVLYSIIFTVARRQARAVRTVKNGASKIQRAKPSNSSETKAAKTLGIIIFVYLACWIPLYIGSLSVESFTSSSMVWTVFGWLLYLNSSVNPLIYAIFYSWFRESAKCILTGTIFESSSSRFNLYPESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J4S3</accession>
    <name>A0AAR2J4S3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KCNA3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J4S3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085859.1">
        <property type="protein sequence ID" value="ENSPNAP00000045249.1"/>
        <property type="gene ID" value="ENSPNAG00000030302.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161860"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000012">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000025">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF250">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="18">
        <location>
            <begin position="66"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="465"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="505"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="533" mass="59259" checksum="BF8A03A847D25E92" modified="2024-11-27" version="1">IGGGGRAGGGRSMEIALVSLENGGAHTGSPRSPPPSPWRTEPSARLFDDEALDEAAAAALDVASAERVLINVAGLRYETRLGTLAQFPDTLLGDPEKRIKYFDPLRNEYFFDRNRPSFDGILYYYQSGGKIRRPVNVSIDVFADEIRFYELGDEAMERFREDEGFIKEEERPLPENEFQRQVWLIFEYPESSSPARAIAIVSVLVITISIVTFCLETLPEFRDERELAATTAVSGRTSETNGTRSRPSLTFADPFFIVETTCVIWFTFELLVRFLACPSKSAFSKTVMNVIDVMSIMPYFITVGTELVEQQHGHQHEDGQQQQQQQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADEPESHFSSIPDAFWWAVVTMTTVGYGDMRPVTVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETDQDQASLRDDGPGGRESPTSLRLAGEADNEDGTDNSPAEKPSVKVNSSSSSSVDIKRSLYAFCLDARETDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JD59</accession>
    <name>A0AAR2JD59_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JD59"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076444.1">
        <property type="protein sequence ID" value="ENSPNAP00000049958.1"/>
        <property type="gene ID" value="ENSPNAG00000037551.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286905"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000013">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF391">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="225"/>
        </location>
    </feature>
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            <end position="262"/>
        </location>
    </feature>
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        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="43"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="320" mass="36595" checksum="CA8D76351213F1F8" modified="2024-11-27" version="1">MQDLPVQNFSFTAFKLSGFHDLGQWRPLLFIPYFLMFLLSTTSNSVLIYLIISKRALHTPMCVLIGLMAVVDLCLPVFFVPNMLLSFLFNWNGISLVCCLIQMFCLLYVGTFQSTLLVWMALDRFFAICRPLYYHKYMQMPNFLKFIIVPVIRNIILIITIVSSAGKLSFCATDEIDHCFCEHMALVQLACGDISINNLIGLVATFLIPTADFLFITLSYVIILASVLKSRKSHLKAVNTCITHIIVMTFTLTFALIAFLSYRIRNNFSPSVRVFLSTMYLLFPSCFNPIIYGVRTKEIREQFLKFMNQENSGAANFFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JGT0</accession>
    <name>A0AAR2JGT0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JGT0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056501.1">
        <property type="protein sequence ID" value="ENSPNAP00000049522.1"/>
        <property type="gene ID" value="ENSPNAG00000031367.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000289282"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="303" mass="33867" checksum="EDB902E420A4B48D" modified="2024-11-27" version="1">MTGLKGKQKHMTGLKGKQKHMTGLKGKQKYMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKHMRGLKGKQKHMTGLKGKQKHMTGLKGKQKHMTGLKGKQKYMTGLKGKQKHMTGLKGKQKHMTGLKGKQKYMTGLKGKQKHMTGLKGKQKYMTGLKGKQKHMTGLKDKQKHMTGLKGKQKHMTGLKVFFVHWLSLNCWDIVKLATVVYSFALSSSDCGLNAQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JGY3</accession>
    <name>A0AAR2JGY3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JGY3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054352.1">
        <property type="protein sequence ID" value="ENSPNAP00000049577.1"/>
        <property type="gene ID" value="ENSPNAG00000036245.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="107" mass="12042" checksum="1143D1FA77C4FA45" modified="2024-11-27" version="1">HASPSISVQSVVVRPCEQSQHFIYCPESLENPLCPAQEHAPARCMFWTFYHKVTSVPHAVGLVCLSAGLMFVIAALLWFPIIKDKLKREGDFRNISISQLDLNYGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLA0</accession>
    <name>A0AAR2JLA0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphate regulating endopeptidase homolog, X-linked</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PHEX</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_017573766.2">
        <property type="nucleotide sequence ID" value="XM_017718277.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLA0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045210.1">
        <property type="protein sequence ID" value="ENSPNAP00000050626.1"/>
        <property type="gene ID" value="ENSPNAG00000012478.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439727"/>
    <dbReference type="GeneTree" id="ENSGT00940000157313"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF133">
        <property type="entry name" value="PHOSPHATE-REGULATING NEUTRAL ENDOPEPTIDASE PHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="8">
        <location>
            <begin position="76"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="7">
        <location>
            <begin position="535"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="746" mass="85745" checksum="43B5212779131CCE" modified="2024-11-27" version="1">MEMEMEKLSGRGSKQSAAPTTLMKAALTGSVCLCLALLIAVIAVSQKTTNEEFCLSSECIEAAGSILNKMDLSAQPCEDFYKYACGGWLQENPIPEDSSSYGIYPWLRQNVDLKLKELLEQPTTNSDIEAVKKAKVLYRSCMNETALELLDAKPLLKELRKREFRWPVLGNVLGADVQWDEEKFDLLHTLAQIRTQHSKSVLVRLYVSPDDKNSQRYIIKLDQASLVLSREDYITNTTEAQANREALLRLMVDVPVMLGAPEEAAEQQMKPVLDFEMKLAQIMIPFENRTSESMYNKYSLSKLQRTIPEFNWLSFVRASIDSKLYPDLSISSSEHTIVRVPQYMKDLFKLLNVTDSRTVANYVVWRSVLSRITTLSRRFLYRYLDFARVTTGTTSLTPRWDKCVNYIEGTLMYATGRLFVDKHFQEDKKHMMEELIEGIRWAFIDMLEKENDWMDAETKKKAIQKAHAVLAKVGYPEFILNDTYINEDIKRLSFSEKDYFGNVMQTLRFISQADIGWLRKNVPRTEWFTNPTTVNAFYSSSTNQIRFPAGELQKPFFWGLDYPRSLSYGAIGVIVGHELTHGFDNNGRKYDKDGNLDQWWSNASIASFNDKTQCMIDQYNTYHWKEAGLNVRGKRTLAENIADNGGMREAFRAYRRWIDKERSGVEEPLLPGVGLSNNQLFFLSYAQVRCNSYRPEAARDQIVSGAHSPPKYRVIGAMSNYEEFRKAFNCPETSLMNRGAESCRVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLF9</accession>
    <name>A0AAR2JLF9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Suppressor of IKBKE 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Suppressor of IKK-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SIKE1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIKE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLF9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080637.1">
        <property type="protein sequence ID" value="ENSPNAP00000051127.1"/>
        <property type="gene ID" value="ENSPNAG00000020975.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018003"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008555">
        <property type="entry name" value="SIKE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12186">
        <property type="entry name" value="SIKE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12186:SF4">
        <property type="entry name" value="SUPPRESSOR OF IKBKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05769">
        <property type="entry name" value="SIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="214" mass="25003" checksum="B67EB729D4017C26" modified="2024-11-27" version="1">MSCTLEKVLSDARTLLERLKEHDTAAESLIEQSSTLSQRVHSMKEVGNALPDKYMEESAGYQELSKYKPHVLLSQENTQIKELQQENRELWLSLEEHQYALELIMGRYRKQMLQLMMGKKELDTKPVLSLHEDHAKEVQSQLERICEMGQVMRRAVQVDDQHYCSVKERLAQLEIENRELRDLLAISKNTLKPHREEVSQPNTEPKSKTNQKHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JSJ3</accession>
    <name>A0AAR2JSJ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LUC7-like (S. cerevisiae)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LUC7L</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037393087.1">
        <property type="nucleotide sequence ID" value="XM_037537190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JSJ3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060653.1">
        <property type="protein sequence ID" value="ENSPNAP00000054993.1"/>
        <property type="gene ID" value="ENSPNAG00000033918.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413553"/>
    <dbReference type="GeneTree" id="ENSGT00950000183213"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="128"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="273"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="355"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="47245" checksum="CA99F94F12BCDB20" modified="2024-11-27" version="1">MFIEPFGQGKLYPLIFNKATEGLELQAGYSMGERARSDESRQRVKFTDERVCKSHLLNCCPHDILSGTRMDLGECSKIHDLALRADYEIASKERDLFFELDAVDHLESFIVDCDRRTELAKKRLAETQEEISAEVAAKAEKVHELNEEIGKLLAKAEQLGAEGNVDEAQKILQEVEKVRSKKKDAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIQIREKLEQLKKTVLDKQERRNQERLKRREEREREERMKKRTRSRSRERRRSRSRDRDRDRDRERERERDRDRERERRRRRSRSASREKRRTRSRSRSRERRRRHRTRSRSRSRHSREHSHKSSRDRDRERDGRRGSSERRSDGLNGRVESHRSGDREAGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JX16</accession>
    <name>A0AAR2JX16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JX16"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060650.1">
        <property type="protein sequence ID" value="ENSPNAP00000056540.1"/>
        <property type="gene ID" value="ENSPNAG00000034627.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043636051" description="Immunoglobulin V-set domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="2">
        <location>
            <begin position="42"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="127" mass="14399" checksum="156B32BAFA7C9FFB" modified="2024-11-27" version="1">MFWSPTSVFNNLYLCALFFSSDSLGQVVLTQSGDQTVQPDQTVTITCKHKPEVNCVNNQEEKKSCMSWYHQIPGEAPKLLTYFTDKRASGVSSRFSGSERLNFQLTISGVQPEDTHREKEGSLQPFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JYD2</accession>
    <name>A0AAR2JYD2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JYD2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067069.1">
        <property type="protein sequence ID" value="ENSPNAP00000055267.1"/>
        <property type="gene ID" value="ENSPNAG00000030422.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182977"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="6">
        <location>
            <begin position="84"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="6">
        <location>
            <begin position="193"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="364"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="365"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="597" mass="65747" checksum="0E3896372F89082C" modified="2024-11-27" version="1">MHHLQGKFELLNYSRSKKEVGMPSLSGLDPAEDTVLSQSPRVSDRVYVEEPLISELVLVCDAELRMKVLLIFTLFLISVGGGESGRVMGYSGGGVLINCRYETRYTSNPKYLCKGPWSNCGDRIKTGVKNKWTNRGRFSLFDDTRAAQFWVVIRELTVEDSGTYKCGVDLDLAIDIYKPVELNVEEDLSYEKSISVTGHGGGGVNISCKYPQSHSSDPKFLCRRLGIVNCSHKTSVKESRRWINEGKLILHDDGKQIFTVVINHLTEGDSGEYWCGAESDWTSDHGYKVYITQINLTVTEAPATSPSSSTPQSTETPSSTTTVIPSFKTSTVSSVITVVSVVVMLLLIGLLIFIVTFRKRRKTQGLETNTQFSRESTGNFENDPTSCSLQPTKNTNQSDSAYQSLDPTTTQSDSAYQSLDPTTNQSVSAYQNLDPTTNQSDSAYQSLDPTTNQSVSAYQNLDPTTTQSDSAYQSLDPTTNQSVSAYQNLDPTTNQSDSAYQSLDPTTNQSVSAYQNLDPTTNQSDSAYQSLDPNTTQSDSVYHSLNPNTNQSDSVYHSLNPTTTQFDSAYQSLNPNTNQSDSVYHSLNPNTNQSDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JYT7</accession>
    <name>A0AAR2JYT7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JYT7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083902.1">
        <property type="protein sequence ID" value="ENSPNAP00000055422.1"/>
        <property type="gene ID" value="ENSPNAG00000035079.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177372"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="1">
        <location>
            <begin position="29"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="121" mass="13707" checksum="330B74603A78DE70" modified="2024-11-27" version="1">MDPAGLEQRLTSQDQKLDELVQLVQGLLQRISTPAAPHAVPERYDGSPGMCNSFLMQCTLYITHNPSQFRLETDKVHFVISLLTGRAKEWATALWTVNSAILQSKSRFLSQFKPVFGDEWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K336</accession>
    <name>A0AAR2K336_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K336"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066794.1">
        <property type="protein sequence ID" value="ENSPNAP00000058738.1"/>
        <property type="gene ID" value="ENSPNAG00000035669.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177162"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd07699">
        <property type="entry name" value="IgC1_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000283">
        <property type="entry name" value="Immunoglobulin kappa constant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig_C1-set"/>
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        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
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        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="9"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="109" mass="11588" checksum="9CC71913E13524D3" modified="2024-11-27" version="1">TPNSAVPAPILTIFPPSSEELKSNKATLVCLANDVSSALADVRWLVDGKAVSSGVTTGSAAQQPNKKFRLSSYLTIERSEWDKDKAITCEVSAASKATTKEIKKSECSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K6X6</accession>
    <name>A0AAR2K6X6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transposase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K6X6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086455.1">
        <property type="protein sequence ID" value="ENSPNAP00000060058.1"/>
        <property type="gene ID" value="ENSPNAG00000036929.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286914"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:DNA transposition"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057667">
        <property type="entry name" value="HTH_SB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
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        <property type="entry name" value="Tc1-like_DDE_dom"/>
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        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
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        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="transpos_IS630"/>
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    </dbReference>
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        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="37"/>
            <end position="87"/>
        </location>
    </feature>
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        <location>
            <begin position="104"/>
            <end position="174"/>
        </location>
    </feature>
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        <location>
            <begin position="184"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41573" checksum="9799229E00C26304" modified="2024-11-27" version="1">MTQKGPPVCNLISVQIQLLRDGLRCWLREYWGANSIMKSKEHTRQVRNMVLEKFKAGLGYKKISQALNISRSTVQSIIRKWKEYGTTVNLPRQGRPPKLTGRTRRSLIREAAKRPMVTLDEMQRATAQVGETVHRTTISRALHKCGLYGRVARRKPLLKENHKKSRLQFARSHVEDTANMWKKVLWSDETKIELFGHNAKRYAWRRNNTAHHSENTIPTVKYGGGSIMLWGCFSSAGTGKLVKVDGKMDGAKYRAILEENLLESAKDLRLGRRFTFQQDNDPKHKARTTMEWFKKKNIHVLEWPSQSPDLNPIENLWQDLKTAVHKRSPSNLTELELFCKEEWARISVTRCAKLVETYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K8R3</accession>
    <name>A0AAR2K8R3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K8R3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053770.1">
        <property type="protein sequence ID" value="ENSPNAP00000058879.1"/>
        <property type="gene ID" value="ENSPNAG00000035544.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230338"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C_TYPE_LECTIN_2"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="136"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="47"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="253" mass="29273" checksum="FCB3DDF30CCAC22A" modified="2024-11-27" version="1">MCLYLFSEPSTAGRRCFILTTACLGLLCVLLLAAITVLWIKFTLAQRDQLETHYTNLTRERDQLQTSYTNLTKERDQLKTSNTKLSEAINHLELSYISVMKERDELQKKLSSHRNRTKQFQDHKFKRSNFTKLEEVHFECTIKENSQTIWSESRQDCRNRGADLVTINSREEKVCVRGQRAWIGLTDSEKEGVWKWVDGSALTTTFWGHSEPNSNAGDEDCVITGEGSDPVKNWADYPCIDRFGWICKKSIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KE79</accession>
    <name>A0AAR2KE79_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Shootin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017538528.1">
        <property type="nucleotide sequence ID" value="XM_017683039.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KE79"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078832.1">
        <property type="protein sequence ID" value="ENSPNAP00000062633.1"/>
        <property type="gene ID" value="ENSPNAG00000006375.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411462"/>
    <dbReference type="CTD" id="562403"/>
    <dbReference type="GeneTree" id="ENSGT00980000198769"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044295">
        <property type="term" value="C:axonal growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031252">
        <property type="term" value="C:cell leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001224">
        <property type="term" value="P:positive regulation of neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024849">
        <property type="entry name" value="Shootin-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46606">
        <property type="entry name" value="SHOOTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46606:SF4">
        <property type="entry name" value="SHOOTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="349"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="434"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="508"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="257"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="573" mass="64578" checksum="688F3D5FEF9CA20C" modified="2024-11-27" version="1">MLSPGIDINILDSEDENITSSEDEQDIQVEILENQRDEANQRLSKMEEASSQLLKEIDMLEMQFQIERSCRVSAEAFALKVTKENKVLKRRSQALLPLIPELPEKLTALNADLEDDPDSDLSTSAETEEEPLVKSQAQIRDLQSSVDQLLGEKMQLCEQIEVLKKEKDELKEQLAAEVEEKEAILRKLAKQSRTVNKMKRVSQLVTEEFAEMTQKLEIEQGLRHHAEIFAHQVLVKQRETHRQSMAIEQTSEMDTQLKQALEQVAQISKALEEIRLHHQQQRSQSQSAMEEFSALSELQVISARLETSEKERIKVENQLRDSQRTMTALQEELKQLQVGVKEALPLESAQSNKGNASLAPPFPPPPPPPPPPPPPPPPPPPPPVLSTAPTDPLDALRNRKKGGSTATDSKKPVLSMEMKARAVDEMMERIKKGIVLKPTQKPPQASSEEETAWKDQRSEKRKSAVLELKGMLDSMKRPGHRRVMSRKRISRNVGEAELQLVLQRRRRAIGDEKGAHPAATPKTQDPLPAAPASSSSPWLAEGDTAPVLRRLKQNQEKRSSRIRASECILWEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KGN9</accession>
    <name>A0AAR2KGN9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">deoxyguanosine kinase</fullName>
            <ecNumber evidence="7">2.7.1.113</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DGUOK</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2'-deoxyguanosine + ATP = dGMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19201"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57673"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.113"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCK/DGK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.113" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017571807.1">
        <property type="nucleotide sequence ID" value="XM_017716318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KGN9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051442.1">
        <property type="protein sequence ID" value="ENSPNAP00000061281.1"/>
        <property type="gene ID" value="ENSPNAG00000019358.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438482"/>
    <dbReference type="CTD" id="1716"/>
    <dbReference type="GeneTree" id="ENSGT00940000159627"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004138">
        <property type="term" value="F:deoxyguanosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000461">
        <property type="entry name" value="Deoxycytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF8">
        <property type="entry name" value="DEOXYGUANOSINE KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="12">
        <location>
            <begin position="38"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="42"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="253"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="276" mass="32109" checksum="2446D1AE068FF598" modified="2024-11-27" version="1">MGFHTVTVALHLTRRFFSNSSQCRLIHRMDGISRVKRVSIEGNIAVGKSTFAKLLQNAGQNWEVTPEPVSKWQNVEEATSQIVLSPQQTTSNLLQMMYQDPKRWSYTFQTFSCMSRMRTQLQPPPAKLMQSRDTAVQVYERSIYSDRYIFALNSFELGALNSTEWAVYQDWHSFLVEQFGPKVQLEGIIYLRASPQVCMERLGKRGREEERGLQLDYLDTLHVQHENWLINKSTKVHFDHLKNLPVLVLDAGLEFEKDPEVQARLIKEVKIFFHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KI50</accession>
    <name>A0AAR2KI50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">N-acetylneuraminate-9-phosphate synthase</fullName>
            <ecNumber evidence="8">2.5.1.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Sialic acid synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O = N-acetylneuraminate 9-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:80835"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58557"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:231734"/>
            <dbReference type="EC" id="2.5.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:80836"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-III AFP family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.57" evidence="8"/>
    <dbReference type="RefSeq" id="XP_037388601.1">
        <property type="nucleotide sequence ID" value="XM_037532704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KI50"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074858.1">
        <property type="protein sequence ID" value="ENSPNAP00000061786.1"/>
        <property type="gene ID" value="ENSPNAG00000031721.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108443442"/>
    <dbReference type="CTD" id="322780"/>
    <dbReference type="GeneTree" id="ENSGT00390000011081"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047444">
        <property type="term" value="F:N-acylneuraminate-9-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006054">
        <property type="term" value="P:N-acetylneuraminate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11615">
        <property type="entry name" value="SAF_NeuB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000144">
        <property type="entry name" value="sialic acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1210.10">
        <property type="entry name" value="Antifreeze-like/N-acetylneuraminic acid synthase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036732">
        <property type="entry name" value="AFP_Neu5c_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006013">
        <property type="entry name" value="Antifreeze_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051690">
        <property type="entry name" value="PseI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013132">
        <property type="entry name" value="PseI/NeuA/B-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006190">
        <property type="entry name" value="SAF_AFP_Neu5Ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057736">
        <property type="entry name" value="SAF_PseI/NeuA/NeuB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42966">
        <property type="entry name" value="N-ACETYLNEURAMINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42966:SF1">
        <property type="entry name" value="SIALIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03102">
        <property type="entry name" value="NeuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00357">
        <property type="entry name" value="ANTIFREEZIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51269">
        <property type="entry name" value="AFP III-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50844">
        <property type="entry name" value="AFP_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0047">Antifreeze protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AFP-like" evidence="11">
        <location>
            <begin position="294"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="359" mass="39967" checksum="663FC3FD8D9D5224" modified="2024-11-27" version="1">MPLKFELCPGRMVGGSQPCFIIAEIGQNHQGDLETAKKMIKMAKDCGADCAKFQKSELEHKFNRRALERPYTSQHSWGKTYGEHKRHLEFSHEQYRELQRYAAEVGIFFTASGMDEMAVEFLHELNVPFFKVGSGDTNNFPYLEKTAQKGRPMVVSSGMQSMETMRRVYQTVKKHNQNFCILQCTSAYPLEPEDVNLRVITEYQKEFPDIPIGYSGHESGISITVGAVALGAKVVERHVTLDKSLKGSDHAASLVPSELAELVQAIRTVERALGSGVKRMLPCEVPCHDKLGKSVVAKTAIPKGTELTLDLLTVKVAEPKGVAPEDIFQLVGKKVLVDIEEDESVTEDMVESYGKKSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KLJ7</accession>
    <name>A0AAR2KLJ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Distal membrane-arm assembly complex protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP synthase subunit s-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Required for the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Involved in the assembly of the distal region of complex I.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with incompletely assembled mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP synthase subunit s family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017579010.1">
        <property type="nucleotide sequence ID" value="XM_017723521.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KLJ7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054931.1">
        <property type="protein sequence ID" value="ENSPNAP00000062976.1"/>
        <property type="gene ID" value="ENSPNAG00000036482.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108443096"/>
    <dbReference type="CTD" id="55101"/>
    <dbReference type="GeneTree" id="ENSGT00940000160500"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000168">
        <property type="entry name" value="Distal membrane arm assembly complex 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF169">
        <property type="entry name" value="F-BOX AND LEUCINE-RICH REPEAT PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00057777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00076566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="255" mass="29249" checksum="7997F97BEC67101F" modified="2024-11-27" version="1">MAAPVLCQMRRRCPVVAYLSVVRREYASSPPSPPSLFSRFLFQLNQHFHDVEHLINWSSWLRNRVVRRKNVFYGYTQKRFGVNVAAAYYILGLGGGFRFAGHSDWFRPDSRGKFSYDFLNTPNSTIEEIDLSRTLINHAGLENIVSQNELRSLSLQGCPEVDDWFLARLHVFGESLQWLDLSHCPHITVGGLAALQHLRKLRRLDVSSLPRLQNPGLVRILLEEMLPHCEVIGADYEQGLILTDNVEEAEPVRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNG7</accession>
    <name>A0AAR2KNG7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNG7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055767.1">
        <property type="protein sequence ID" value="ENSPNAP00000063651.1"/>
        <property type="gene ID" value="ENSPNAG00000031786.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244806"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21063:SF4">
        <property type="entry name" value="CD48 ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21063">
        <property type="entry name" value="LFA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="155"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="302" mass="33672" checksum="2515068890C0DEA2" modified="2024-11-27" version="1">MQTKASTSQSIMSRRERFWIYSSLSNCFFMILFVLLIHLIIVNDVRAAGQDKVETIEVKEGVNVTLRTNLTGIKGDDIVLWSFEGTRIAQQINKDIVRYFKETFKTGLKLDNQTGSLTISNIRTTDSGAYVQQLISEKPSIKKFSVTVYAPVPVPAIEYGFRQNQSGTKIQSNPNCSMVCSVRNALKVTLSWFRGSQLLSNITNSSNSSLSLTLEIKHGDNDTYICVAANPISNQTALVKRTCTQNSSTIGKATPSPHAEKRPQHLWLIPVLLVLFIVSVVMYVVHEKFYKGKQKGPSNAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KQS9</accession>
    <name>A0AAR2KQS9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BTB/POZ domain-containing protein KCTD7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">KCTD7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017544464.1">
        <property type="nucleotide sequence ID" value="XM_017688975.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KQS9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062639.1">
        <property type="protein sequence ID" value="ENSPNAP00000066635.1"/>
        <property type="gene ID" value="ENSPNAG00000033750.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108415927"/>
    <dbReference type="CTD" id="154881"/>
    <dbReference type="GeneTree" id="ENSGT00940000161327"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060081">
        <property type="term" value="P:membrane hyperpolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18366">
        <property type="entry name" value="BTB_POZ_KCTD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000046">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057891">
        <property type="entry name" value="BTB_KCTD7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057890">
        <property type="entry name" value="KCTD7/14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF122">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25611">
        <property type="entry name" value="KCTD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="93"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="331" mass="37418" checksum="E54096E0D51EA704" modified="2024-11-27" version="1">MQQNGAGDTPNGAGDTPVLRRFGPEGRAGPQPRVMVVFSAASESEESVDAGTGLGGAAAGLEEQEPRRAATGSPLRSLGKTLHSFSPAQEFPEVIPLNVGGTYFTTRLSTLRRYEDTMLAAMFSGRHHIPRDPEGRYFIDRDGAYFGDILNFLRGGELPQRDRVRAVHREAQYYAIGPLLESLEDTQPLTGEKVRQAFLDLLPYYKDNLERIVEIAKLRAMQRKARFAKLKVCVYKEEMPITPYERPLFNSLRFERSESEAKLFEHHCEVDVSFGPWEAVADVYDLLHCIVSDLAERGITADQQCIGVCDKHLINHYYCKRPIYEFKITWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KRB2</accession>
    <name>A0AAR2KRB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KRB2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068556.1">
        <property type="protein sequence ID" value="ENSPNAP00000064942.1"/>
        <property type="gene ID" value="ENSPNAG00000032494.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156835"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000016">
        <property type="entry name" value="B-cell linker protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051751">
        <property type="entry name" value="Immunoreceptor_sig_adapters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098:SF2">
        <property type="entry name" value="CYTOKINE-DEPENDENT HEMATOPOIETIC CELL LINKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098">
        <property type="entry name" value="SH2 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
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    </dbReference>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="3">
        <location>
            <begin position="21"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="126" mass="14153" checksum="6E396BB2C5C95CFA" modified="2024-11-27" version="1">KSRSTPSICNTMTASLPTWGLSSKGDAEGAFLVRDCSRNTTNEPFVLAVFYENRVFNIQIRFCKETSKYTLGTRIKTNDTFDSVADIIKFHSIFPIVLIDGRNPSATSHPKRHCVLIFLHLQPAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KTF4</accession>
    <name>A0AAR2KTF4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ankyrin repeat and sterile alpha motif domain-containing protein 1B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ANKS1B</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000070976.1">
        <property type="protein sequence ID" value="ENSPNAP00000065401.1"/>
        <property type="gene ID" value="ENSPNAG00000018412.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154572"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01274">
        <property type="entry name" value="PTB_Anks"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09499">
        <property type="entry name" value="SAM_AIDA1AB-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09500">
        <property type="entry name" value="SAM_AIDA1AB-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000045">
        <property type="entry name" value="Ankyrin repeat and sterile alpha motif domain-containing protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000099">
        <property type="entry name" value="ankyrin repeat and sterile alpha motif domain-containing protein 1B isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033635">
        <property type="entry name" value="ANKS1/Caskin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041880">
        <property type="entry name" value="SAM_ANKS1_repeat1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041882">
        <property type="entry name" value="SAM_ANKS1_repeat2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF3">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="126"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="159"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="192"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="818"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="889"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="7">
        <location>
            <begin position="1063"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="295"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="501"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="620"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="752"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="951"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1198"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1231"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="505"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="671"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="698"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="971"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1232"/>
            <end position="1248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1323" mass="146020" checksum="408C128A98CF28A8" modified="2024-11-27" version="1">MGKEQELLEAARTGNVAVVEKLLSGKKGLLGSGSGSIQLPGLLSMWRGLNVNCADSSGYTPLHHAALNGHRDVVLKLLQFEASTNVADSKGCFPLHLAAWRGDVDIVRILIHHGPSHCRVNEQNHEKETALHCAAQYGHSEVVSVLLQELTDPSMRNSRGETPLDLAALYGRLQVVRMLLSAHPNLMSLNTRIHTPLHLAARNGHHSTVQALLEAGMDVNTETEKGSALHEAALYGKMAVVRLLLDSGIDVNITDCQGKTALEILKEHPAQKSQQITALIQDYMMAESDRCSFPEEPVRQCPVPTPRTSIPSPAASPSLRHKSKHELTGELSKLLNEIKMCRDRDYSFEELCQTISSHSQSMESFSVRTSGRPSPPLPPAVEEEEKVCGPSGLWEALTPCNGCRNLGFSQDSKRCVEIIQSPSLDVFLPEDEDNPYESVATAVTRKPCSLDIHLLNTCHSSHVPVSDVEQRNHCDDYTGLTPDCSPPSPDTALKNIERVIRPQPKQRTSLSSSQDVQRPVQNHSGEPSEVSSSLGYASFSTSPPASPPISPANYSTGSAEDCALTGTHEVLEQRECVLRDERRDSHIPEQFAGLLHGSSPACESPDNPYQLYGRVRKLCAPEPPQRHGNLLRAPEPAGPPFPRPSATQASPPKGQKEPKPQVVYRTIFHTRVNQSPLTAQSESDSVSNGEARSNNGPSSTAVSSSVSSTAGYEERACTLGRMRSVPRNVLDLQLSRNFSKSDSNLVAVSPIEEEQWSSRGQIQNSPGKGGSPTRLERTPSFTAEWEEIDKIMSSIGAGIGTELDENKEDHSGPRCPVQSVGQWLDNIGLVQYENHLLANGFDNVQFMGSNVVEDQDLLEIGILNSAHRQRLLQAIRLLPRVRPIGYDGNNPTSVAEWLESLELGDYTKSFLINGYTSMELVKKIWEIELINVLKINFIGHRKRILASLGDRLHEDPPQKPPRAISLREPSGNHTPPQLSPSINQTAYPNAGSLDVQHLIMQADARRRRNDNYFEDVPRSKLERQMAQQGEWCEPITLRPPNEATSSTPVQYWQHHPEKLIFQSCDYEAYYLGSMLVKELRGTESTQDACAKMRKSTEQMKKVPTIVLSVSYKGVKFIDATNKNIIAEHEIRNISCAAQDPEDLSTFAYITKDLKSSHHYCHVFTAFDVNLAYEIILTLGQAFEVAYQLALQARKGGHGSSTLPESFDSKPSKPIPKPRANIRKSVSAGCTHTHTHTHTHTHTHPHTHTRSPTPTYRLTLTGSVSFRLSTLLTFPAANYPPHLYVLALWLMRAALCLMLLNPILHPHLSPKASSLIACFWPVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L173</accession>
    <name>A0AAR2L173_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CDX4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Caudal homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L173"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051106.1">
        <property type="protein sequence ID" value="ENSPNAP00000068126.1"/>
        <property type="gene ID" value="ENSPNAG00000018771.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165070"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000089">
        <property type="entry name" value="Caudal type homeobox 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006820">
        <property type="entry name" value="Caudal_activation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047152">
        <property type="entry name" value="Caudal_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24332">
        <property type="entry name" value="HOMEOBOX PROTEIN CDX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24332:SF15">
        <property type="entry name" value="HOMEOBOX PROTEIN CDX-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04731">
        <property type="entry name" value="Caudal_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="150"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="152"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="213"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="274" mass="30525" checksum="E56A879EFFAE520F" modified="2024-11-27" version="1">MYVGYLLDKEGNMYHHQGPVRRSGINLPPQNFVSTPQYSDFSGYHHVSGVDPHAQAAGPWAAPYGAPREDWGAYSLGPPNTISAPMSNTSPGQVSYCSPEYPPMHNPASAVLPPPQDNISAPPLSPDRERRNSYQWMSKTVQSSSTGKTRTKEKYRVVYTDHQRLELEKEFHFNRYITIRRKSELAVNLGLSERQVKIWFQNRRAKERKLIKKKLGQSDGSGGSVHSDPGSVSPLPLPVPPTRNEHLTVHEEHTAGHSHSVNSNLAAHGGQTDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L2V6</accession>
    <name>A0AAR2L2V6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vestigial-like family member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">VGLL3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the vestigial family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L2V6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081200.1">
        <property type="protein sequence ID" value="ENSPNAP00000068711.1"/>
        <property type="gene ID" value="ENSPNAG00000017119.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063353"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011520">
        <property type="entry name" value="Vg_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15950">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15950:SF16">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07545">
        <property type="entry name" value="Vg_Tdu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="37794" checksum="5330153229B5543F" modified="2024-11-27" version="1">MSCLDVMYHQNYGAHHYLPATSVAAAAAYKAAYYHHHHHQQQKKFSAYSKMQGSVDLPVHCGQSKQAEKSRAEPDLQREEEQTAAEEARCKETQPAEAEYLSARCVLFTYFHGDIGDVVDEHFSRALSQPSAFTNDTKSNRHVSGVSWKDGGSLTDGQCGSLPSSLWSSSYPSQATSPCISSVHGDFPATATFHTADTGLWSGHSLTQPGLPPPSTLTDSWPYSLASQSGATYPHVHEMYPHVHPCHPHPHAHPMLHHAHSTALDPRFSPLLLPGMRPSCSPSSNPEGVKTELEPNGSTTPSWPASFHGSVDIYDTGKKRNKYSLCTYLSFLNASILEYAFKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4I1</accession>
    <name>A0AAR2L4I1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM227 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L4I1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076064.1">
        <property type="protein sequence ID" value="ENSPNAP00000069552.1"/>
        <property type="gene ID" value="ENSPNAG00000012140.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169177"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029417">
        <property type="entry name" value="FAM227"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33560:SF1">
        <property type="entry name" value="PROTEIN FAM227A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33560">
        <property type="entry name" value="PROTEIN FAM227B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14922">
        <property type="entry name" value="FWWh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="468" mass="53025" checksum="FDB9772FFF7176C3" modified="2024-11-27" version="1">MEEVNRLCVRILVHQEDVKENPVSARKRQDSLRESAQSPVMCLLGTMATLSEAISQLPLLPRPGHPMQIRSADGKIQPESTADDEKTEKPRSLPWSTSHKSRVATEKRGERMKMVELYQCPVFTDIPVPLPHDTAYATIVTHVVQAQRHLVHKPSLKASFQTILSSSTVEHFVIDSFWWFFLYNFQPDRKTQDRLFCRIAENYISILTQNLSTRSGTTFLRWSGPPWTLTQQYFSSTLSQTLYCCFCCSFPQSCSFRNQAFLTALCSTAYQWTGGICPAPEVYKTWDFEALEPDEATGVSESEKRQKESGSTLTLSESMFSSTSLFNPPIHNQHGSLTGPKKSRRGSDANEASIICSRNNMAITSHKSISEDDVIGTDLKALTDHVNYTSLRESHAVYKCVELRQCVFNVWGNSPLVQHYMTTLNLKQTVGHDLLVRRSQIQKLPPYPYMLNPIHSEISSFLHTHSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4P2</accession>
    <name>A0AAR2L4P2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Charged multivesicular body protein 1B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CHMP1B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L4P2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061857.1">
        <property type="protein sequence ID" value="ENSPNAP00000069684.1"/>
        <property type="gene ID" value="ENSPNAG00000008037.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182832"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23675" checksum="8A037E6D6C5A0A30" modified="2024-11-27" version="1">IEKNGLTFPLLCYCKHLFNLKFAAKELQRNSKKCDKEEKVEKAKVKKAIQKGNMEVARIHAENAIRQKNQSVNFLRMSARVDAVAARVQTAVTMNKVSTHMHTFTMNLYTMDSSCCFLIGQISSLMDKFEHQFETLDVQTQQMEDSMSSTTTLTTPQNQVDTLMQEMADEAGLDLNMELPQGQTGTVASSVASTEQDELSQRLAKLRDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L587</accession>
    <name>A0AAR2L587_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">FAT atypical cadherin 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576847.1">
        <property type="nucleotide sequence ID" value="XM_017721358.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000056639.1">
        <property type="protein sequence ID" value="ENSPNAP00000071710.1"/>
        <property type="gene ID" value="ENSPNAG00000020416.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441695"/>
    <dbReference type="CTD" id="406172"/>
    <dbReference type="GeneTree" id="ENSGT00940000157733"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000152">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000154">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000015">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000021">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000026">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000037">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000041">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000051">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000052">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000064">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000065">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000067">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000071">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000075">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000079">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000080">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000089">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000107">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000161">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000053">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000058">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000059">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000061">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000084">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000151">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000057">
        <property type="entry name" value="protocadherin Fat 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="70"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="146" mass="15830" checksum="FCD63A178ECDED0E" modified="2024-11-27" version="1">GTAPVAVGVDSLVECHQDNLCFSLPAYETCEPAKGDPQHPPYYSHFVQVIHSTLKVHEPLGSLQPSNGAFEVTVYNGTDNTKIPFIMKSTDTIGKLKKAYIKKRPHMIGSLNLVFNGKPVQAEKTLGELGVKTGATFITFQRCIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L8U5</accession>
    <name>A0AAR2L8U5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integrator complex subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cleavage and polyadenylation-specific factor 3-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">INTS11</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L8U5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077164.1">
        <property type="protein sequence ID" value="ENSPNAP00000071129.1"/>
        <property type="gene ID" value="ENSPNAG00000022204.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157644"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16291">
        <property type="entry name" value="INTS11-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000002">
        <property type="entry name" value="Integrator complex subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000003">
        <property type="entry name" value="Integrator complex subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041897">
        <property type="entry name" value="INTS11-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048662">
        <property type="entry name" value="IntS11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF37">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21386">
        <property type="entry name" value="IntS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="12">
        <location>
            <begin position="41"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="13">
        <location>
            <begin position="270"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="661" mass="74706" checksum="67C6D760E792D916" modified="2024-11-27" version="1">LGAIQNHIQHILHQSEEHLHHVKNHSVHANGSFECAGQDVGRSCILVSVGGKNIMLDCGMHMGYNDDRRFPDFSYITQNGRLTDFLDCVIISHFHLDHCGALPYMSEMVGYDGPIYMTHPTKAICPILLEDFRKITVDKKGETNFFTSQMIKDCMKKVVPLNLHQTVQVDDELEIKAYYAGHVLGAAMVLIKVGSESVVYTGDYNMTPDRHLGAAWIDKCRPDILISESTYATTIRDSKRCRERDFLKKVHETIERGGKVLIPVFALGRAQELCILLETFWYRMNLKAPIYFSTGLTEKANHYYKLFITWTNQKIRKTFVQRNMFEFKHIKAFDRSYADNPGPMVVFATPGMLHAGQSLQIFKKWAGNEKNMVIMPGYCVQGTVGHKILNGQKKLEMEGRATVMMTVPTTGRQRACTNVVAFSFPCLLLNLEVKLQVEYMSFSAHADAKGIMQLIRMAEPRNMLLVHGEAKKMEFLKDKIEQEFSISCYMPANGETTTVTTNPSVPVDISLNLLKREMALGGPLPDPKKPRTMHGTLIMKDSSLRLVSPEQALKELGLSEHQLRFTCRVHLQDPHSDSDTLSRIYSHLKSVLKGYAVQHLPDGTVMVESIVLKVSSSGEDPNLKVILLSWSYQVNVEQRGTHSDWDCVPAVRTTDKNMLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LTD4</accession>
    <name>A0AAR2LTD4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Family with sequence similarity 83 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM83 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LTD4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049967.1">
        <property type="protein sequence ID" value="ENSPNAP00000079863.1"/>
        <property type="gene ID" value="ENSPNAG00000037301.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157889"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012461">
        <property type="entry name" value="SACK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF29">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07894">
        <property type="entry name" value="SACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffolding anchor of CK1" evidence="3">
        <location>
            <begin position="14"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="374" mass="43389" checksum="59B8CE3E41CD70BF" modified="2024-11-27" version="1">HSKSTQTSSKSKHEKPEDYIQPHYKESYRLAIDSLVSGGKHVYQEFIKAEKIMSFLSEDEISFIIQNAEQLPVCVQPEEVERPADEEASTGTYWPTHSDAPPPDLDLGWPEVIHSRLQTNIDLLYHPPRLNSSTIKEVVRKHIQDARQVIAIAMDVFTDVDIFKEVVDAAVKGVPVYILLDHNQFKTLSFLQNMRVRTVKGQEYLCRSGAKFHGGMGQKFLIVDCHTVFFGSYSFMWSFEKIHLSMVQVITGKLVESYDEEFRTLYARSTIPEELQPQEISSERRLNGKMEGYLGHPTKMFERSDQLRHTLDSVYRQTCERQPGFKSTLDDLPNPIAHQSRFLQETTDFNKRELEECRNKFRTRTAECRQRKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LU21</accession>
    <name>A0AAR2LU21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576944.1">
        <property type="nucleotide sequence ID" value="XM_017721455.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LU21"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087061.1">
        <property type="protein sequence ID" value="ENSPNAP00000078234.1"/>
        <property type="gene ID" value="ENSPNAG00000018162.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441763"/>
    <dbReference type="CTD" id="563785"/>
    <dbReference type="GeneTree" id="ENSGT00940000157186"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11262">
        <property type="entry name" value="Sema_4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001170">
        <property type="entry name" value="Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (Semaphorin) 3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000027">
        <property type="entry name" value="Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4Ga"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000033">
        <property type="entry name" value="Semaphorin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF17">
        <property type="entry name" value="SEMAPHORIN-4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043512807" description="Sema domain-containing protein" evidence="17">
        <location>
            <begin position="24"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="705"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="19">
        <location>
            <begin position="37"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="573"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="654"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="781"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="663"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="788"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="869" mass="97477" checksum="6FE7C60968FB5762" modified="2024-11-27" version="1">MMSRDVAWLSCLQLLCGMSSLWAYPFRPPLDLDITPRTTVLSNGLWGCRRFSGTALNYSTLLLEEEAGVLYVGARGALYALQADNISNFSRTIDWEAPEDQKQLCLNKGKDNKTECFNHIRFLQRFNSTHLYTCGTHAFSPLCAYIDDKDFRISSVFEEGRERCPYDPAKGYTGLLIDGQIYTASQYEFWSSPDIRRNSPNPTLRTEEAPTRWLYEADFVGSALVRETVNSSVGDDDKIYFFFTEKSQEHSPYFSHSRVARVGRVCKSDRGGLLTLQKKWTSFLKARLVCSLPDYEFHFNVLRSVFVLEGLRTQDTVLYGIFGLEWKNVKASAICRYSMTDIQQAFDGPYMESQEDTSAKWTEYSGKVPEPRPGSCITDRLRARGINFSTDLPDDVLHFVRRHPLMAKQILPVGGRPLLFRRSVDYSKIAVHRVTALDGQTYHMLFIGTDEGWLQRAVEVKSQLHIIEELQLFEGPQPVDSIVISQKQMSVYVGSQSAVVQLPLSTCHRYSSCFDCVLARDPFCAWDGEECVEITSHTDRSNLTQDILNGNEGCENSAADDLVLHRVRSVMAGDDVLLQCELSSNLATPQWTLHGQELQGYGLNSGYRIGTDGLLVIEARPDQSGDYSCFALESGVRVPVVIYTLTIRHDLPPVPPIDMTHPPHQSGTTHRTAQTSNRSNLTLHPAPAPHPPQLEFLSLRNMEAMYLSLITILGGLCLVLTVVLLYVGFCVQTRRGKYSLRAAAQAERKRSGHMELKTISSQCNGKVDTHDGLLQIVPGEAQASPNKDLPPAPPLPPPPPNSESEYTNGLSATLPSVLRKMNGNSYVLLRQSDGDTTSPLYYSFTEELNRILEKRKHTQLCNKPDESSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LXV2</accession>
    <name>A0AAR2LXV2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LXV2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043750.1">
        <property type="protein sequence ID" value="ENSPNAP00000079477.1"/>
        <property type="gene ID" value="ENSPNAG00000033683.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170695"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="8"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="95" mass="11006" checksum="AACF64AF1DA4B17B" modified="2024-11-27" version="1">MQENGQSRLQQVQKDVDDVKVIMLDNLNKATEREGKLGDLEDRADVLLQEVKLVQKSRHFIFISYGILYVEYVLNSADVLTYFHLEKQQSMSTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZD3</accession>
    <name>A0AAR2LZD3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LZD3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049567.1">
        <property type="protein sequence ID" value="ENSPNAP00000079706.1"/>
        <property type="gene ID" value="ENSPNAG00000032566.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000141530"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="55" mass="6554" checksum="3DE45929060376F2" modified="2024-11-27" version="1">MPRHLISDTHEWMNEIPTVPTYYLAKPQPRDRAWQNQRGKKTLLSLTLVCRCEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M5G9</accession>
    <name>A0AAR2M5G9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M5G9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049684.1">
        <property type="protein sequence ID" value="ENSPNAP00000082251.1"/>
        <property type="gene ID" value="ENSPNAG00000034826.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="110" mass="12113" checksum="67E03FF80E62110C" modified="2024-11-27" version="1">FPKGLSAPTLTVLPPSSVELQQGKATLVCLANKGFPSDWRLSWKLDGRSWSWGVSQSPGLLQKDDGLYSWSSTLTLQGDQWLKKTVTCEATKDSQPSVSQTLRREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M6I0</accession>
    <name>A0AAR2M6I0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Zinc finger E-box-binding homeobox 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Smad-interacting protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Zinc finger homeobox protein 1b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ZEB2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin. Represses expression of MEOX2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 24">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M6I0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058545.1">
        <property type="protein sequence ID" value="ENSPNAP00000083002.1"/>
        <property type="gene ID" value="ENSPNAG00000023882.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183208"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048023">
        <property type="term" value="P:positive regulation of melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000013">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000082">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000105">
        <property type="entry name" value="Zinc finger E-box binding homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000117">
        <property type="entry name" value="Zinc finger E-box-binding homeobox 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051574">
        <property type="entry name" value="ZnF_E-box_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391">
        <property type="entry name" value="HISTONE H4 TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391:SF11">
        <property type="entry name" value="ZINC FINGER E-BOX-BINDING HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15 24" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16 24" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18 24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="225"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="26">
        <location>
            <begin position="667"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="1022"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="1050"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="27">
        <location>
            <begin position="1078"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="24">
        <location>
            <begin position="669"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="24"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="717"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="775"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1142"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="364"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="732"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="790"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="800"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1164"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1198"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1216"/>
            <end position="1236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00058733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00082894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000083002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1243" mass="138834" checksum="4CAE6465F0A170AE" modified="2024-11-27" version="1">PTFGAVSESRPCELLQQIMADGPRCKRRKQANPRRKNVLNYENVVETGSETEEDDKLMVSEEDVLLNGAGSPASLANHEPAAPPSPRLGHALMTKAEEEDDEMRDSGVEHVWHESDMLRASVDGTDELKDDFDSMGPDATLQPVGNGTVKSLDCTTEFEEFFAKRKLADSESHVVSIAEYLQRGDTAIIYPEAPEEIARLGTPEATGPEDIEPTPGTPDAFAQLLTCPYCDRGYKRLTSLKEHIKYRHEKNEENFSCPLCNYTFAYRTQLERHLTTHKSGRDQHQLLNQGAGNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLITVNGRMRNNIKSGSSPTSASSSPTNTAITQLRHKLENGKPLSLHDQSNHLNIKSEPLDFNDYKLMMASHGYGTPGPFLNGGMGGGSPLGVHNSQSPLQHLGMAIEGQMLGYPSLSNNLSEVQKVLQIVDNTVCRQKMDCKPEELSKLKAYMKELGSQIEEKKQGLGSPGGPQTGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLNMDSKRQISNIKKEKSNHTIDLGTEEKMHENNIMFTPFSCQYCKEIFPGPIPLHQHERYLCKMNEEIKAALQPSENILPNKQGMFTEKHAHLLSSIISEKGITSPINPYKDHMSVLKAYFAMNMEPNSEELLKISIAVGLPQEFVKEWFEQRKVYQYANSRTPPLDRNNMEVGLPSTNHTPNKDSINARSPLSLVKPMDRITSPSIAELHNNINNCDAPLRLSKTPQFPGPKHLGEKLDHSRSNTPSPLNLSSASSKNSHSSSYTPNSFTSEDLQAEPLDLSLPKLMKEPKHVLSIKSQPKPNSVPLDHNPITSPREHLEEPLNLAYLSKKDFGSPNGNGNLDKSTSPMFGINPFAAKPLYTSLPPQSAFPPATFMPPVQASLPGLRPYPTLDQMGFLPHMAYTYATGAASFAEMQQRRKYQRKPGFQGELLDGTPDYLSGLDDMTDSDACLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREARDKGHLEPTELLMKRAAYLQGLSPAGYPERVPERDPILRDSINGGARERPAKEAEEAGGGAYSKLGRREEEFEEDEEESENKSVDTDLDTARDEEDNADHSMDDSSVDGKTETKSDHEDITEDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNZ9</accession>
    <name>A0A3B4BNZ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FBXO42</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037388237.1">
        <property type="nucleotide sequence ID" value="XM_037532340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNZ9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000196"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014260.2">
        <property type="protein sequence ID" value="ENSPNAP00000000196.1"/>
        <property type="gene ID" value="ENSPNAG00000000134.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414095"/>
    <dbReference type="GeneTree" id="ENSGT00440000039706"/>
    <dbReference type="OMA" id="WILDMEQ"/>
    <dbReference type="OrthoDB" id="9973021at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22110">
        <property type="entry name" value="F-box_FBXO42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052821">
        <property type="entry name" value="F-box_only_SRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46432">
        <property type="entry name" value="F-BOX ONLY PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46432:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13415">
        <property type="entry name" value="Beta-prop_FBX42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="55"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="546"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="602"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="727" mass="77132" checksum="613BC3B222C6300A" modified="2018-12-05" version="1">MSRSSDSEDGCFVDMDTEDSGVALAGHGEDPAVEMKAGDVAGAADGSQQPGRNGQTAMGELPEEVLEYILSFLSPYQEHKTAALVCKQWYRLIKGVAYQCYHGFLKAVQEGNIQWESRTYPYPGTPITQRFSHSACYYDSNQSMYVFGGCTQSSCNAAFNDLWRLDLNSKEWIRPLASGSYPSPKAGATLVMFRDLLVLFGGWTRPSPYPLHQPERFFDEIHTYSPSKNWWNCIVTTNGPPPMAGHSSSVINNNMIVFGGSLGARQMSNEVWVLDLEQWSWSKPTIKGPSPHPRGGQSQIVVDDETLLILGGCGGPNALLKDAWLLHMGAPTWSWQQLRVENEEHGAPELWCHPACRVGQCVVVFSQAPSGRAPLSPSLNSRPSPISATPAPLGPEPPSLRSQSPVRSGSSGAAALVLGSAEEAPCVNGRWGTLRPRPRGGARDGSPSSSSSSQQPSPLQAPDSPPLPPLLNGSSPSPRTSPSQAASPPSRPHGHAHLPTDYGWESPPPAVPHDHPSTNGLHTPPRTPPGAVSPAALRRGLEAVKALSSSSSSSLPSSSSSSLAQPGGSSTLKALSSSSPSSSSVPQPGASSAPSSTPPAPSSSSSPPSQAGAAADGHSIPPIARRLGHHPPQSLNVGKPLYQSLNCKPMQMYVLDISRAKTDAVVSWRIYGSGGAPSAVTGPPETSLHTVVQGRGELIIFGGLMDKKQNVKYYPKTNALYFVRAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPE9</accession>
    <name>A0A3B4BPE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPE9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013802.2">
        <property type="protein sequence ID" value="ENSPNAP00000000465.2"/>
        <property type="gene ID" value="ENSPNAG00000036425.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176185"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000465.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11934" checksum="D68D840AD0079DE8" modified="2024-11-27" version="2">MTIIWIIIFTLTPGELFLSYLFSLDDDTAARVFTITITDLRPEDGGRYWCVVKRPKSLTDLYTEILLLVKSVQTQSTPPSSSPSTGISSFSQSVFLLVDPSVHPVNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPK7</accession>
    <name>A0A3B4BPK7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="28">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="28">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="28"/>
    <dbReference type="RefSeq" id="XP_017580226.1">
        <property type="nucleotide sequence ID" value="XM_017724737.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017580227.1">
        <property type="nucleotide sequence ID" value="XM_017724738.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPK7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000520"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013716.2">
        <property type="protein sequence ID" value="ENSPNAP00000000520.1"/>
        <property type="gene ID" value="ENSPNAG00000000301.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443857"/>
    <dbReference type="CTD" id="570240"/>
    <dbReference type="GeneTree" id="ENSGT00940000155181"/>
    <dbReference type="OMA" id="LSWHVFE"/>
    <dbReference type="OrthoDB" id="3256376at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048403">
        <property type="term" value="F:brain-derived neurotrophic factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060175">
        <property type="term" value="F:brain-derived neurotrophic factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990416">
        <property type="term" value="P:cellular response to brain-derived neurotrophic factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099551">
        <property type="term" value="P:trans-synaptic signaling by neuropeptide, modulating synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000239">
        <property type="entry name" value="BDNF/NT-3 growth factors receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000034">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000033">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000163">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020777">
        <property type="entry name" value="NTRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF575">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01939">
        <property type="entry name" value="NTKRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-0221">Differentiation</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="22" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11 25 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 28" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 31" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 28 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="18" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017200836" description="Tyrosine-protein kinase receptor" evidence="31">
        <location>
            <begin position="23"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="401"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="33">
        <location>
            <begin position="184"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="32">
        <location>
            <begin position="508"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="24">
        <location>
            <position position="648"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <begin position="514"/>
            <end position="522"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25 27">
        <location>
            <position position="542"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with SHC1" evidence="26">
        <location>
            <position position="486"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with PLCG1" evidence="26">
        <location>
            <position position="789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="794" mass="89318" checksum="EA549AC617F382C6" modified="2018-12-05" version="1">MVGMARWSFYVVLMGLLKLASSCPASCSCNTSRISCVDQEPGIDEFPILVPETDMENITDIYITKQNRLFTITEDHMKFYTNLRNLTVTKTHLTSVSSKAFANNAKLQYLNLRDNNLSTLSWTVIRNCNMSTLLLAGNPLWCACENMWIKAWLEDSERDQLQCLQEGGRVKTLSKLTVPSCEYPRVEVIPNVVTQMAGSDAIVACSASGAPAPVVSWNVNSTDHPPLTTAYETKTSDLQSMLTLTGLSPSDNGREITCNAENIVGQNEASVTLNIRFRPIILQLYEPERSHDWCIPFSVTGNPKPELQWYHQGQPLEEQDYIKTQIHESTESEYHGCLQLDIPTHIHNGVYTLVATNEFGEDQKNVTAHFMHIPDVEHSGTDEFYYDTTTPPEIPPQEDKVAVYVVVGIAGVALTGCILMLIFLKYGRSSKFGLKGSSSVISNDDDSASPLHHVSNGNNTPSSSEMGPDAVIIGMTKIPVIENPQYFRNPSSMLKSDTFVQHIKRHNIVLKRELGEGAFGKVFLAECYNLSPDQEKILVAVKTLKEASESGRADFHREAELLTNLQHEHIVTFYGVCVESDPLIMVFEYMKHGDLNKFLRAHGPDAVLMADGQQSMLVELTQSQMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGENLLVKIGDFGMSRDVYSTDYYRVGGHTMLPIRWMPPESIMYRRFTTESDVWSLGVVLWEIFTYGKQPWYQLSNNEVIECITQGRVLQRPRTCPKEVYDLMLGCWQREPYMRLNIKEIHNLLQSLAKASPVYLDILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BU78</accession>
    <name>A0A3B4BU78_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ATP5F1C</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Subunit gamma, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the central stalk subunit delta, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Interacts with FLVCR2; this interaction occurs in the absence of heme and is disrupted upon heme binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BU78"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011100.2">
        <property type="protein sequence ID" value="ENSPNAP00000002050.2"/>
        <property type="gene ID" value="ENSPNAG00000008530.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006837"/>
    <dbReference type="OMA" id="MQITSAM"/>
    <dbReference type="OrthoDB" id="239812at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000007">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000013">
        <property type="entry name" value="ATP synthase subunit gamma, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 15" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11 15" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002050.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="293" mass="32132" checksum="B80FCAB9904DD53F" modified="2024-11-27" version="2">MFARASAVVFLPQCGQVRSMATLKDITIRLKAVKNIQKITKSMKMVAAAKYARAEKSLKPARVYGTGALSLYEKADIKAPEDKNKHLLIGVSSDRGLCGAIHSSVAKAIKSEIAKLAGAGKEVMVVNIGDKLRGLLYRTHGNHILINCKEVGRKPPTFTDASIIATELLDSGYEFDQGTIIYNRFKSVISYKTDEKPVYSMDTVANSENMGVYDDIDADVLRNYQEFALVNIIYYGLKESATSEQSARMTAMDSASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUA7</accession>
    <name>A0A3B4BUA7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">All-trans-retinol 13,14-reductase</fullName>
            <ecNumber evidence="15">1.3.99.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">RETSAT</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2</text>
            <dbReference type="Rhea" id="RHEA:19193"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:52075"/>
            <dbReference type="EC" id="1.3.99.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.23" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUA7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002090"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011033.2">
        <property type="protein sequence ID" value="ENSPNAP00000002090.1"/>
        <property type="gene ID" value="ENSPNAG00000001094.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017613"/>
    <dbReference type="OMA" id="DYIYPKG"/>
    <dbReference type="OrthoDB" id="38045at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051786">
        <property type="term" value="F:all-trans-retinol 13,14-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000362">
        <property type="entry name" value="All-trans-retinol 13,14-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052206">
        <property type="entry name" value="Retinol_saturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091:SF1">
        <property type="entry name" value="ALL-TRANS-RETINOL 13,14-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091">
        <property type="entry name" value="BLR7054 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017438352" description="All-trans-retinol 13,14-reductase" evidence="19">
        <location>
            <begin position="23"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="564"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="607" mass="67597" checksum="3798CEBE2262B579" modified="2018-12-05" version="1">MWVAVGLVIVLLVVLLLKNVVGLSGPSPFDFDSREPLKPMVLDRKEKNKVLKQGFLASRVPQDLDAIVIGSGVGGLGVAALLAKVGKKVVVLEQHDRAGGCCHTFSEKGFEFDVGIHYIGDMLENEMFRCIVDQMTNGQLQWEAMDNPYEEVVLGPLENRRVYHIYSGEHRFTQELKKCFPGEEKAIDEFMRLIKKCGQGVWFMAVLKLIPSLIAKFLVYSGLINRLSYFFRYGTRSLAEVVNGLTGNKDLRAVFCYIFGTYGKPPKEASFSMHSLLLCHFMPGAWYPKGGASEIAFHMIPVIEKAGGAVFVRAPVNRILLNDAKEAIGVSVMKGHEEVHVHAPIIISDAGIFNTYQQLLPKEVQTMSAIQKQLSLVKHGEGALSVFLGLDGTKEELGLEAKNYWIYSESDLDALMEGYLKGNRDEALKSLPLVFLASPSAKDSTWEQRKPGKSTLTVVSFAPYSWFEEWKDDKVKNRGADYKELKDTLINSLLEVVYQIFPKTKNKIEYMDAGTPITNQHYLASPKGEIYGADHDTSRFSTDVCISMRPQTPIKNLYLTGQDVFLCGFAGALAGAMLCGSAVLNRNLYVDVFNLCRKLKQAKTKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUM8</accession>
    <name>A0A3B4BUM8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucuronokinase with putative uridyl pyrophosphorylase</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUM8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010702.2">
        <property type="protein sequence ID" value="ENSPNAP00000002286.2"/>
        <property type="gene ID" value="ENSPNAG00000008753.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00540000072704"/>
    <dbReference type="OMA" id="KYMEWLI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047940">
        <property type="term" value="F:glucuronokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000090">
        <property type="entry name" value="glucuronokinase 1-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000324">
        <property type="entry name" value="Glucuronokinase with putative uridyl pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053034">
        <property type="entry name" value="Glucuronokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38710">
        <property type="entry name" value="WITH PUTATIVE URIDYL PYROPHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38710:SF1">
        <property type="entry name" value="WITH PUTATIVE URIDYL PYROPHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="2">
        <location>
            <begin position="525"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002286.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="619" mass="69808" checksum="740AA13938342555" modified="2024-11-27" version="2">MICVLLVAGHGTVLETQIKNDVTGLYGHLAGVPKALLPGVGGKKILDFWWETVNTRQLFSEVYLVTNADKYKHYERWATANDFPVENVVNDGSTTLDDRLGAVADLELAIRSRQLQDDIMVIAGDMLCADQNFDIAQVIRFFKSKSGELAIYYELEEGEKCCSRGIVEVCPESHRITRFLEKPQEGVTASRLASVVFYCLRKETLSYLSDFLLQQPNVEDKTFGRFWEWLINEEKLPVYGMKLPTGFQLIGQVGLSDYTKWLAHYSAKQQESPAKPVTCRSYARVGLMGNPSDGFNGKTIALTISNFWAEVTLVESQTLVLLPHPLNDPTEFGSLQDLFRISRKEGYLGGLRLLQATCKKFYQFCSKQGIALTKQNFTLKYDTNIPRQVVSLIGPVCAIVSATLKCLMKFYNITEDDLPKPIRANFILNVETDELFITAGLQDRVVQVYEGLVYMDFSKQLMEERGYGEYIPLDMSSLPTFWLGYLGDPSDSGRIHSNVRQRWLNGETDVVEAMKRFAELTDEARAAFHTKDWPKLAQLMDENFELRRSIYTDDCLGPGNLKMVQLARQFGSAVKLPGSGGAVVGLCMDPDRLVKQTLLLPQRHIFVTCSTHLFDPDCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BV31</accession>
    <name>A0A3B4BV31_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BV31"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002436"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010437.2">
        <property type="protein sequence ID" value="ENSPNAP00000002436.2"/>
        <property type="gene ID" value="ENSPNAG00000008821.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163324"/>
    <dbReference type="OMA" id="IMCTIAT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000096">
        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF886">
        <property type="entry name" value="GROWTH HORMONE SECRETAGOGUE RECEPTOR TYPE 1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="323" mass="37280" checksum="DE4B1E621B1975E9" modified="2024-11-27" version="2">MQNSVINSNLSLNVLTELSRSLFLKMTLVQVLVGIFLYVNCLMIFTFLNKAVFREDTRYILFAQTLFVDSALMVLTDMLLIVNLYQYRIHMIPCIILCTIATIFTNCTPLTLVAMCLERYVAICMPLRHADISTSRNRCFGFLFIWGLSSIAPFFTLLAYLFVVPPAALFTYALCSAELMMGEEWQAQTRAIILQILFLFMIIIIVFTYIKIMIAARAASSENKKSTNKSLRTVILHAFQLFLCIVNFLTPYIEMPFWKVDFLVFHNVRFSNFIVFVIAPRCLSPLIYGLRDEKFFLLLKHNALCGLTAFYKHKIKEQTNFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZM9</accession>
    <name>A0A3B4BZM9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZM9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004055"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007653.2">
        <property type="protein sequence ID" value="ENSPNAP00000004055.1"/>
        <property type="gene ID" value="ENSPNAG00000010527.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286905"/>
    <dbReference type="OMA" id="PNMLLGF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15223">
        <property type="entry name" value="7tmA_OR56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF429">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="321" mass="35725" checksum="67D0E40004B9FDC2" modified="2018-12-05" version="1">MLSNFSHTKFIFVGFPEIFQYRRLLFLPFFLSYITVLVGNSLLLFVIKNTRNLHSPMYMLVSVLAVIDIVVPTAIIPAVLLSFLFDLNEITLAGCLTQMFLIHFFSSVESTILLAMALDRTVAICNPLRYTAIMNSSMFLKLLLFTLIRSGAIMSTLVGLASPLSFCGSNVIHHCYCDHMALVSLACNSTAKNNAMGVAVIICFVGIDISVIFFSYMKILYVVLRAAAGEHSSKAFHTCGTHLIVMMCFYFVGSVTFLSRNLSIPIPVDVNTFLGVTYIVFPACVNPIIYGVRTKEIRNALLKIFKTRVNRVTVKVSTVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0F8</accession>
    <name>A0A3B4C0F8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arsenite-resistance protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SRRT</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0F8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004139"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007494.2">
        <property type="protein sequence ID" value="ENSPNAP00000004139.2"/>
        <property type="gene ID" value="ENSPNAG00000010629.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005492"/>
    <dbReference type="OMA" id="FEDKIMQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007042">
        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021933">
        <property type="entry name" value="SERRATE/Ars2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
        <property type="entry name" value="ARS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12066">
        <property type="entry name" value="SERRATE_Ars2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SERRATE/Ars2 N-terminal" evidence="7">
        <location>
            <begin position="175"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="SERRATE/Ars2 C-terminal" evidence="6">
        <location>
            <begin position="664"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="287"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="778"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="354"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="386"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="401"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004139.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="883" mass="102146" checksum="90DDEB12A218E7AA" modified="2024-11-27" version="2">MADSDDEYDRRRRDKFRRERSDYDRSREREDRRRDDWSDRCWIFFRLVFICFLFAVNWRPSAREWDRGRERRSRGEYRDYERGRRERFSPPRHDMSPQQKRMRRDWDDHGGDPYHGGYDLGYGGGGGPSYAPPQPWGHPDMHLMQPHHGIPIQARLGNIHDPDLGPPPPVMKTFKEFLLSLDDSVDETESVKRYNEYKVEFRRQQMQDFFLAHKDEEWFRSKYHPDEAGKRKAEAHAALQNRLNVFMYQMENGWFDGISMDIDRAPQILKILDAAVIKMEGGTENDLRILEQPAEEEEEKERLSSSGTTSEPAKRDENRPDEGDRKPSAEKDEKEVSVGVELSKKRKRKHSGDSEDEASASESESDSESDSNSHTSEKPAEREKEGEQEEGEEKEEDEDGEGQRGVKERQEDKERDQEKEEKKAKDDQSPRPRPLHKTSSLFMRSIAPTISKAEIVALCRRYPGFMRVCLSDPQPERRFFRRCWVTFDRAVNIKEICWNLQNIRLRDCELAPGVNRDLARRVRNINGITQHKQILRNDIKLAAKLIHSLDERESLWSPQPSEETQPVELPAQNPILKNITDYLIEEVSAEEEELLGSAGGADAEEGAKEGNPTEITVERDDKLVKVLDRLLFYLRIVHSIDYYNTCEYPSEDEMPNRCGIIHVRGPIPPNRITHREVADWEKTFEEKLVPLFSVKESLSEEEALKMGRKDPEQEVEKFVSANTQELGKDKWLCPLSGKKFKGPEFVRKHILNKHGEKIEEVKKEVEFFNNFLMDAKRPSLPEMKPPPPPGPGQGLLSPGGPPFPPQGPQGLLGFGQPRPPLLGYGGGPPYPPNQYGGGRGNYDNFRGQGGYPGKPRNNRMIRGDPRNIIEYRDLDAPDDVDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0V9</accession>
    <name>A0A3B4C0V9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Interleukin 17a/f2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0V9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007245.2">
        <property type="protein sequence ID" value="ENSPNAP00000004304.2"/>
        <property type="gene ID" value="ENSPNAG00000001451.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156618"/>
    <dbReference type="OMA" id="TKERCNE"/>
    <dbReference type="OrthoDB" id="6093351at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043803284" evidence="5">
        <location>
            <begin position="39"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004304.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="152" mass="17196" checksum="DF4C1446CCEDCAF9" modified="2024-11-27" version="2">MFHSSRRTVSVSTPYLMRRYRVVFCVGLLMLSLTDVKATQKKICDVGLKIPDFHGAEAEMLGGNGNINNRSLSAWNWIPDTSPHRIPGVIFEAQCETHYCTDPNNNLQTELNSVPIYHYILVLHQDKQDRKCFTASYQKVTVGCTCVRAQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C1S8</accession>
    <name>A0A3B4C1S8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567595.2">
        <property type="nucleotide sequence ID" value="XM_017712106.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C1S8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004639"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006670.2">
        <property type="protein sequence ID" value="ENSPNAP00000004639.2"/>
        <property type="gene ID" value="ENSPNAG00000011062.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435944"/>
    <dbReference type="CTD" id="570746"/>
    <dbReference type="GeneTree" id="ENSGT00940000160645"/>
    <dbReference type="OMA" id="CERCACM"/>
    <dbReference type="OrthoDB" id="3214149at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033278">
        <property type="term" value="P:cell proliferation in midbrain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030902">
        <property type="term" value="P:hindbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048385">
        <property type="term" value="P:regulation of retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000035">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000041">
        <property type="entry name" value="Zinc finger protein ZIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041643">
        <property type="entry name" value="Znf_ZIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF8">
        <property type="entry name" value="GLIS FAMILY ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18366">
        <property type="entry name" value="zf_ZIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="286"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="333"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004639.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="386" mass="42912" checksum="8E0F2045C2B18773" modified="2024-11-27" version="2">MLLDSGHHQLSGLSSLNSGAFSPHDRELELAQGYGLLGARAHFGAARADFFLRGGGGAGGFGEAGVSTSAAHHHHQQQHAMFGHGHAQTDSHQGAVGHALFPGFHEQQQHHQPQQQHHQQQHHLHALNGRLDVFARAAAAAAAADPYGQYGLNMNVSAHHAHPFFRYVRQRCAERELELACRWTERENQRERPCGRTFGTMHELVAHVSAEHVGGPSEHSEQHVCRWEECARHGKPFKAKYKLVNHMRVHTGEKPFACPFPGCGKVFARSENLKIHKRTHTGEKPFMCEFSGCDRRFANSSDRKKHMHVHTADKPYLCKLCDKSYTHPSSLRKHMKVHDEQSTLKEDCSPGGSSGYDSSSVVSPGSEAQDVLSPDPPNNNFSEWYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C263</accession>
    <name>A0A3B4C263_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable G-protein coupled receptor 34</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">G-protein-coupled receptor of lysophosphatidylserine (LysoPS) that plays different roles in immune response. Acts a damage-sensing receptor that triggers tissue repair upon recognition of dying neutrophils. Mechanistically, apoptotic neutrophils release lysophosphatydilserine that are recognized by type 3 innate lymphoid cells (ILC3s) via GPR34, which activates downstream PI3K-AKT and RAS-ERK signaling pathways leading to STAT3 activation and IL-22 production. Plays an important role in microglial function, controlling morphology and phagocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552267.1">
        <property type="nucleotide sequence ID" value="XM_017696778.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552268.1">
        <property type="nucleotide sequence ID" value="XM_017696779.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C263"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006202.2">
        <property type="protein sequence ID" value="ENSPNAP00000004916.2"/>
        <property type="gene ID" value="ENSPNAG00000011264.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426943"/>
    <dbReference type="CTD" id="100147937"/>
    <dbReference type="GeneTree" id="ENSGT01110000267167"/>
    <dbReference type="OMA" id="LPYAFMK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045028">
        <property type="term" value="F:G protein-coupled purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14982">
        <property type="entry name" value="7tmA_purinoceptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000150">
        <property type="entry name" value="probable G-protein coupled receptor 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233:SF11">
        <property type="entry name" value="P2Y PURINOCEPTOR 14-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
        <property type="entry name" value="P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="10 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043691514" description="Probable G-protein coupled receptor 34" evidence="16">
        <location>
            <begin position="22"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="51"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004916.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="361" mass="40744" checksum="D880DF6F7999D8C5" modified="2024-11-27" version="2">MAATSLTTALSFTLTVNTTNATSSPCVRTEVPAHPFFIFAYCLVCMVSLALNAITMRVYFCSSQRLQSSVTVYLKNLAAADFFLCLCLPLRIANYASKSLTMRRIYCNFGATAFYFNMYASILFMDYIAANRYLKIVRPVETHALQTARAARYISIVTWVSLSGMASIYFIVFLCTSWTVDPNPNVIGCEALHSSKVNMLYKIIHSVSAIIFVIVLVSLVLLYWGTVRRLQQSQKAQAKPSNHKLNKSKRNMLVLVVVFCVCFVPYHLVRLPYAFLKPLLKHCATVQAFYIVKELTVLISVLNACLDPLIYFIFCKAFRAQLGIRNRKTTSSWKLSDVPGDMRRQSNSDSRVSTLRHQSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2Q4</accession>
    <name>A0A3B4C2Q4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Solute carrier family 23 member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SLC23A3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547152.1">
        <property type="nucleotide sequence ID" value="XM_017691663.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2Q4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006187"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004005.2">
        <property type="protein sequence ID" value="ENSPNAP00000006187.1"/>
        <property type="gene ID" value="ENSPNAG00000012231.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423965"/>
    <dbReference type="CTD" id="151295"/>
    <dbReference type="GeneTree" id="ENSGT00950000182953"/>
    <dbReference type="OMA" id="LTWGLSC"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="63287" checksum="D4977918B8DF55AC" modified="2018-12-05" version="1">MVKFDRCKWMAACSQQDSLDLTEAKRIKRLKVDYSPPLLLNIGLALQHVLVQVSLCTLVVGALVEEAVRERISASVFFYSGICTLLQSYFGSRLPLVQAPSLDFLIPALVLLSAQAACREQCEKSKEFEAPTHPVRELQGMAVVAGLVQFSVGLSGLIGALLTRCGPLILTPVLCIMGFSVYREAALLCSDHWGLALLTVVLLVFLSQHLRSCSLPACLKLSQYPLFSMFSVLLSMLLVWAVCATLFHYGYIRPHSVPELLLKKTNFSTTQIQDWDVPNSSSLLFTDPTTPWLNPPLTGLGLPLLSGRSIAAGVAAGLSSSLSSQAVYVLTARLLKAPTPPAQACNRGLSMEGLGSILAGLLGAPVGLCSSVANACTLGLSQCGSRGTVQLAGVMLLILGVFLRLTQLLTSIPLAIHGAVLSMTYTVATATGITYLQYTDVDSGRNIFNTGFTVFMSLVLPCWFRMRSDFIYTGEPSLDIFLQSCLMLPVFLVSFLAFFLDHTVSGTLCERGFEQDQDTKKIRSLADKQHGASQSQEGMYEPPELVRRVLSLPGLRVIPFFACSSPDIDKIVETSPEMSSLLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4A1</accession>
    <name>A0A3B4C4A1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017572946.1">
        <property type="nucleotide sequence ID" value="XM_017717457.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4A1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005681"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004882.2">
        <property type="protein sequence ID" value="ENSPNAP00000005681.1"/>
        <property type="gene ID" value="ENSPNAG00000011838.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439195"/>
    <dbReference type="CTD" id="55016"/>
    <dbReference type="GeneTree" id="ENSGT00940000159346"/>
    <dbReference type="OMA" id="KWERLHM"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000043">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase march8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45981">
        <property type="entry name" value="LD02310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="21">
        <location>
            <begin position="292"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="477"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="506"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00012483"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS51292"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005681.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="526" mass="58402" checksum="62F42D066A1256ED" modified="2018-12-05" version="1">MPVHQISVVPASKETTSNSRGSSRTREKSKENEGEKAPGRSGSRSSNISKASSSTQGAVLNGASRTSATPSYQDICSTDGPVKFGKDASDLATPIMPVNHASDSAPISRRQVKRRHRRRRESSNGSGAVGCVSECVDHTKRLRGQTHTRVHSDSSSEDDEQQWRDARSWSREKARRRRSSSRQTPKFTAGQDGRDRVELVAVDADEGQKENRSPRVVGATLKSRKRSSIKREEPPSKAASHGRGSRRASEEEQQPITKSYEEQGSGDANMAIPTAQKYANMADQNGAAQQACSDDELEVCRICHCEGDEDCPLITPCRCTGSLRFVHQGCLHQWIKSSDTRCCELCKYDFIMETHLKPLRKWEKLQMSTSERRKIFCSVMFHLVAVICVIWSLYILINRTAEEIRQGKTNNALLRLSPLNAVGVLEWPFWTKLIVVAIGFAGGLIFMYIQCKVYLQLWRRLKAFNRVIFVQNCPDSARNGDEKAPPPPPPINHRNGRHEPPIVPAPQTQINISCPASTGSAEAAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C520</accession>
    <name>A0A3B4C520_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Cholinergic receptor, nicotinic, alpha 4b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572159.1">
        <property type="nucleotide sequence ID" value="XM_017716670.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037401250.1">
        <property type="nucleotide sequence ID" value="XM_037545353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C520"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002885.2">
        <property type="protein sequence ID" value="ENSPNAP00000006818.1"/>
        <property type="gene ID" value="ENSPNAG00000012801.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438688"/>
    <dbReference type="CTD" id="556619"/>
    <dbReference type="GeneTree" id="ENSGT00940000159329"/>
    <dbReference type="OMA" id="MMHRPAT"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005892">
        <property type="term" value="C:acetylcholine-gated channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015464">
        <property type="term" value="F:acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035094">
        <property type="term" value="P:response to nicotine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000014">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000005">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022260669" description="Cholinergic receptor, nicotinic, alpha 4b" evidence="21">
        <location>
            <begin position="23"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="595"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="31"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="244"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="26">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="70363" checksum="68E10F60568DD51C" modified="2018-12-05" version="1">MSPARSIQLVLILYFQHSYVCTLTPARAHAEERLLQVLFSRYNKLSRPVANISDVVLVHFGLSIAQLIDVDEKNQMMTTNVWVKQEWSDYKLRWNPEEYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAQLFHDGRIKWKPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDRAKIDLVSMDSAVDQMDYWESGEWVIIRAVGTYNIKKYECCTEIYPDITYSFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECAEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPRTHRMPHWVRQLFLHLVPRYLFMKRPPASGKRNCRKLIEMMHRPVVPQATLLGTTTLPPLDSPRPDAGMVSAVPLGGLQREPAAKTPLFCSSPSSQYSILQEEPTQVPRTPALRYTQTHNTSASSPSSSLDTALGPLLHTLPLTEVCQFYCHSAESVFVDGKDGLHETESLQHCHKHEGTALQAHCSADGGGGTGQCTRHKLLETQNSSSNCSKEPKTEEVDSPLSQALLRALEGVQYIADHLRAEDSDFSVREDWKYVAMVIDRIFLWMFVLVCILGTAGLFLPPWLAGMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9D5</accession>
    <name>A0A3B4C9D5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9D5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007516"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001709.2">
        <property type="protein sequence ID" value="ENSPNAP00000007516.1"/>
        <property type="gene ID" value="ENSPNAG00000013305.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157482"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071787">
        <property type="term" value="P:endoplasmic reticulum tubular network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF6">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="900"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1009"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="880"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="91"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="381"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="702"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="798"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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            <end position="238"/>
        </location>
    </feature>
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        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
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            <end position="336"/>
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            <end position="837"/>
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            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU210713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1069" mass="118191" checksum="3FB8D0ECC2CC2BF9" modified="2018-12-05" version="1">MDADKRVVLGSDRPSEGVATSLRFSSQPSSFSSVCYGNESSGPPGGNVDRSFQEKRELFESSVGSQRQDSSPIKVSPVSERIKALEALASKQNDSDWNDGGFPHFRERHYEKSPTEMHGISSRSSFKKRATSTEQESPESPFEVLGDARQGSDFEDTADWMKAHLPPAPDFGTGESDFDETKESAVMQESPSTEIKGKDTDVPEVPESFVGVPDEFMDTPIEVPEQMVDDNKEAKQDSVEEESEFDLRFLPTAYMWDKQEKSDMDTQYPSNIPETQDSEKAVSSAPPIGFESSSVPSPPPVAQMDSSTKQQSQAGGSEPAEILEVDSSGESDDTVIEDASGGQPHAGPAALEFVPCIDKVTLSDDKEKPGILVPIINVIETEEQNISDDEMVQEEEEDDERYQIIQEPTTQASKSSDEHSEVSKSEEVPLNEDDISSPTKVMESDVDFSPKHKIKDDVSEDLLNQTCVDSKGSTLEDSREPVLQNVYEPQKESYVNIVKPDKTAEITQDLQHNYEDLSNEIESSDIDTYLDHYSSQELALKDQLNSGVLSENDGELYDLQKSNTLERSQNDTMFAQQSIEETIVDEIDEQLNESKQNIDPVPEPLLAGIADVHNESVLPQNEVPSPELEDQCVDLEDRTRVTAPGSSSLVEEKDYISQTIVSPPSIQENTPAPFPSFHDDPSGKISEVISDFVSSDLNEGLVISSSEPDDVPDIEMSPERTSEPESVEPECSVMTASDSFVEFMRECLKSQQDEQPESLGLGYVTKDPTSVVPSPPAMIMDLEQERLTICALKELGSSQEQEDEINVSNKTTSVSSKEGSQPPSKSESSVTSVSTPQHLPNEYQPETSLAKEVEAIDIWVAEAYHLAEHVLAAILTHLSVYELVHWRDPKKTGVVFGTSLLLLLSLAAFSVISVISYLLLALLCVTITFRIYKSVIQAVQKSSDGHPFKALMEKDVSVPPETFRKHVDVCLTYINRALKQMSRLFLVEDLVDSLKLAVVMWLLTYVGAVFNGITLLILADILLFSVPPLYEKNKTQIDHYIEIARTQFNTTIAKLQEKLPGAVKRSKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CCG7</accession>
    <name>A0A3B4CCG7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Solute carrier family 13 member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC13A2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559756.1">
        <property type="nucleotide sequence ID" value="XM_017704267.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CCG7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009223"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015499.2">
        <property type="protein sequence ID" value="ENSPNAP00000009223.1"/>
        <property type="gene ID" value="ENSPNAG00000014757.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431247"/>
    <dbReference type="CTD" id="9058"/>
    <dbReference type="GeneTree" id="ENSGT01030000234550"/>
    <dbReference type="OMA" id="MTSHEIG"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015139">
        <property type="term" value="F:alpha-ketoglutarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015138">
        <property type="term" value="F:fumarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017153">
        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071285">
        <property type="term" value="P:cellular response to lithium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="485"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="67175" checksum="96B9EE54658B84C1" modified="2018-12-05" version="1">MPNNSVFCRLIKETGRFLWTYRNYLIIFITPLVFLPLPVIIPTSEAKCGYIIIVMALYWCTECMPLAATALLPVILFPLLGIMESGKVCVLYLKDSNMLFIGGLLVAIAVEHWNLHKRIALKVLLIVGVRPALLMLGFMSVTAFLSMWISNTATTAMMVPIAQAVLEQLSNTEAERDERELREGRDNQAFEMAEVTTTKLPLDNFTKQQSQDPESGYDLALEQRRKDREAKYLLLNKGMSLSVCYSASIGGTATLTGTTPNLILKGQMDDIFPENGDVINFASWFGFAFPNMVLMLVLSWLWLQCMYLGLNFKKSFGCGLNTEMDREAYKVMKEEYKKLGNMKFAEVGILTIFVLLVVLWFSREPGFMPGWASELNRNGQFVTDGTVAILMSTLLFIIPSQLPCISSYNAEDDQDDAGENNTRQTERKLKAPEALLNWKVVHERMPWNIVLLLGGGFALASGSEVSGLSIWLGESLAPLKSIPPFAISLLLCLLVGTFTECSSNTATTTLFLPILASMATAIELHPLYVMLPCTICASLAFMLPVATPPNAIAFSYGTLKVVDMAKAGFVLNIIGILCINLGINTWGTAMFRLDTFPSWANVTHGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEI1</accession>
    <name>A0A3B4CEI1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uroplakin 3b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">UPK3B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017555049.1">
        <property type="nucleotide sequence ID" value="XM_017699560.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEI1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010357"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035686.2">
        <property type="protein sequence ID" value="ENSPNAP00000010357.1"/>
        <property type="gene ID" value="ENSPNAG00000015903.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428514"/>
    <dbReference type="CTD" id="105375355"/>
    <dbReference type="GeneTree" id="ENSGT00940000153392"/>
    <dbReference type="OMA" id="IYTCYDT"/>
    <dbReference type="OrthoDB" id="9945328at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072046">
        <property type="term" value="P:establishment of planar polarity involved in nephron morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009952">
        <property type="entry name" value="Uroplakin-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024831">
        <property type="entry name" value="Uroplakin-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15446">
        <property type="entry name" value="UROPLAKIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15446:SF2">
        <property type="entry name" value="UROPLAKIN-3B-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07353">
        <property type="entry name" value="Uroplakin_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017231864" description="Uroplakin 3b" evidence="3">
        <location>
            <begin position="20"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="32059" checksum="DF0E3B201E22A09A" modified="2018-12-05" version="1">MEAYIVLCFISMLSVGIQAQSTVNCTPVVTPDLAARITTNSVILNPPSCCFGSLTGLNCTSDKCEVWLVAAVDTGVNNFNTDKTSTDFLSKSPYPSAFSNSNPKNYFLTKAGLQNDFPCPSSSTASYFRVGADGNCITTSCNGVLPQGSTASFKYLLVDPASKTLLAETKWSDNITLYTSKDPGSISDSFAGRSGAMVVITAILSVAMALLLLLLIIAVALCCCGGEGKEQRPASQRLGSVMGSFRIPRYDTHHLKDPAPYDNPAYERERKYTTADTLPKSSATTAVNPASQDDIKMQKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CF13</accession>
    <name>A0A3B4CF13_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CobW C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SIMIBI class G3E GTPase family. ZNG1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CF13"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035319.2">
        <property type="protein sequence ID" value="ENSPNAP00000009456.2"/>
        <property type="gene ID" value="ENSPNAG00000014933.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00640000091523"/>
    <dbReference type="OrthoDB" id="258627at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140827">
        <property type="term" value="F:zinc chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="8">
        <location>
            <begin position="263"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009456.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="369" mass="41237" checksum="D9430E056054BBFE" modified="2024-11-27" version="2">MTMKRVLMDEEDDCPELVPIEEKSTTPAAQIPVTIITGYLGAGKTTLLNYILTEQHNKRIAVILNEFGEGSALEKSLAVSQAGELYEEWLELRNGCLCCSVKDNGLKAIENLMEKKGKFDYILLETTGLADPGAVASMFWVDAELGSDLYLDGIITVIDAKYGMQHLTEEKPDGLINEAARQIALADLTIINKTDLVKETELSQLRDTVRSINGLVRILETQRSRVDLSQVLDLHSFDTKDGIRLTEKLQLVKTRQPHLDKSILTVTFEVPGSVSEDHLNVFIQNLLWEKMFKNKEGLAMNVIRLKGIVSLQGKQKKAILQGVHELYELDETPEAWADGEPSVNRLVFIGRNLDGDILKKEFLSLVSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CF99</accession>
    <name>A0A3B4CF99_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Proto-oncogene tyrosine-protein kinase receptor Ret</fullName>
            <ecNumber evidence="15">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">RET</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation. GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways. Acts as a dependence receptor via the GDNF-GFRA1 signaling: in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling: involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Involved in the development of the neural crest. Regulates nociceptor survival and size. Phosphorylates PTK2/FAK1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CF99"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015943.2">
        <property type="protein sequence ID" value="ENSPNAP00000009546.2"/>
        <property type="gene ID" value="ENSPNAG00000015122.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158499"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014707">
        <property type="term" value="P:branchiomeric skeletal muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048484">
        <property type="term" value="P:enteric nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901166">
        <property type="term" value="P:neural crest cell migration involved in autonomic nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072114">
        <property type="term" value="P:pronephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05045">
        <property type="entry name" value="PTKc_RET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000190">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase receptor Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000234">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase receptor Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041163">
        <property type="entry name" value="Ret_CLD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040667">
        <property type="entry name" value="Ret_CLD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041317">
        <property type="entry name" value="RET_CLD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055162">
        <property type="entry name" value="RET_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016249">
        <property type="entry name" value="Tyr_kinase_Ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF485">
        <property type="entry name" value="PROTO-ONCOGENE TYROSINE-PROTEIN KINASE RECEPTOR RET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17756">
        <property type="entry name" value="RET_CLD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17812">
        <property type="entry name" value="RET_CLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17813">
        <property type="entry name" value="RET_CLD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22540">
        <property type="entry name" value="RET_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000631">
        <property type="entry name" value="TyrPK_receptor_Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="225"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="735"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="885"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="769"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="17">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000631-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000631-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009546.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1125" mass="127676" checksum="F016DD60C97B45AF" modified="2024-11-27" version="2">SAGASSGSSNQQCHHSDLYKCKCIQICLHLHVFVTGSSGLYFPQSTYSETIYVGQPAGAPVLQVHSMRERPSEEPYFCLSWRNIQKPPLYAAWFHLDPLTGVLYMNKTLEWTDFDSLCNIMQMLTLKVAAAPVSQKATCIHHSRVDVSLTFINATAPSCGQKMEKLCFPDGDISSHIKENRLPGALRHHSSKAMYYFIFNTLINYCIFNTYMLVLLSTTDSPVPFAVNNATSELVVTAPVDREEKELYRLIIVCNVRTADMLHRLDTPLLINIYDEDDTAPYVNGTDSEDVLIEFSRSEGTVFGHVFVYDRDTTPSYPKDQTENKFVGTLMTNDSWIKDTFTILHGFREEKAMFGNVRGTVHEYKLSLSRNVLVDERRSVQLDYLVNDTTYPGTEGTVMLHFNITILPVPIQFSNITYIFCPFLVFRILQIGKVCVDNCLKFKGIDIVYQLEISDRNVSSDVLSCYTTVNVARSLEEMSGVLYVNDTKPFYRPECQELQYVLVAQEQQKQLQTKTHLLIIFDGEGKPRSLTCAEKRQRGECETFKGMGAPTGRCHWRQGTDKGISENYSTCSPDLRTCPDGYCDVIESKNMSICPQDCTRVPIIGGHERGLMLGIKAGYGTCYCYSEKCFCEQDEMEEPICDDMCKTIIATAVLLSFIVSILLSSYFIHRYHKNTPKPPIASAEMTFRRPAQSYPISYSANNVRRSSLDSMENQVAIDTFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASHSELRDLLSEFTLLKQVNHPHVIKMYGACSQDGPLYLIVEYAKYGSLRNFLRESRKVGPSYMGSDANRNSSYLENPDERALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRDVYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIAPERLFNLLKTGYRMEKPENCTEEMYSLMLRCWKQEPDKRPTFSEISKELEKMMVKSRDYLDLAASTPADALLYDDALSEEDTPLVDCNNAPLPRTLPSTWIENKLYGMSYPNWPEKSPVQLNRLDATNPVFTRYANDSVYANWMALPSPAKILDKLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJN6</accession>
    <name>A0A3B4CJN6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein regulatory complex protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coiled-coil domain-containing protein 164</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DRC1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the nexin-dynein regulatory complex (N-DRC) a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Plays a critical role in the assembly of N-DRC and also stabilizes the assembly of multiple inner dynein arms and radial spokes. Coassembles with CCDC65/DRC2 to form a central scaffold needed for assembly of the N-DRC and its attachment to the outer doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DRC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573539.1">
        <property type="nucleotide sequence ID" value="XM_017718050.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017573540.1">
        <property type="nucleotide sequence ID" value="XM_017718051.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJN6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011598"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036188.2">
        <property type="protein sequence ID" value="ENSPNAP00000011598.1"/>
        <property type="gene ID" value="ENSPNAG00000017266.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439582"/>
    <dbReference type="CTD" id="92749"/>
    <dbReference type="GeneTree" id="ENSGT00940000153804"/>
    <dbReference type="OMA" id="LDFMMAR"/>
    <dbReference type="OrthoDB" id="10260459at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003352">
        <property type="term" value="P:regulation of cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039505">
        <property type="entry name" value="DRC1/2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039750">
        <property type="entry name" value="DRC1/DRC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029440">
        <property type="entry name" value="DRC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21625">
        <property type="entry name" value="NYD-SP28 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14772">
        <property type="entry name" value="NYD-SP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14775">
        <property type="entry name" value="NYD-SP28_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein regulatory complex protein 1/2 N-terminal" evidence="12">
        <location>
            <begin position="83"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein regulatory complex protein 1 C-terminal" evidence="13">
        <location>
            <begin position="612"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="152"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="676" mass="79011" checksum="9D34450BD5F80406" modified="2018-12-05" version="1">MQAENSAGPSVESENPEERITARRLRIAARNEAKKRQELEVDSQEKEDIEEEARTSQTQVEQSERRMVKLQTDGTELVTNILVAADARESKRRAALEEARRLRVEKLENEAKSSLEKFEEVTRKWTAATAKTIPQDLRDALNSQQQLCALLIENKNKLINELQQELKASDDRYVKDLKKQTEDVDIMIERMQEQITSLMKSYRQELDLIENSFDDERKTLLAGNKKKWEQQRKERGNKELEYLLQRMKQVEEHEVLLHRLRVEHAEEYNMIKTKLETDVQILEQQLQQMKATYQLNQEKLEYNIHVLQKHNEENIITRSQQKRKIRRQQDVLNNLKIKCANQEKQSHEENQLLTDDYKRAMQQYKDMQKKMRHFAAVDAKRFDEVWLMNEAEAKALVHRALEIDRLIHEQQLGLAWFPPSLPFMESSGPVQPRRQVQRTSRQVAAQVLSGMGRKGDTEAEREGSTVEGSESTGVDHKTVKRLLELLCDETGFLIESKLLKLLSSLEKDEQSFMKLDSIFSAVGIENEEDVYKMAEFFVKYKLHQKDQPKEDISISNNGELVPEKDSDLIHPNDVLRALRAFTAQYCKPRDDPSWQQSSVLALQERDDSEDAAYWESMAKVISESKLKVWDALEAALKKYHTVLTERSKLLVDTEHLKQQNSELRMLLHQYQLKGQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CL30</accession>
    <name>A0A3B4CL30_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP synthase membrane subunit DAPIT</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017565538.1">
        <property type="nucleotide sequence ID" value="XM_017710049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CL30"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011546"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018525.2">
        <property type="protein sequence ID" value="ENSPNAP00000011546.1"/>
        <property type="gene ID" value="ENSPNAG00000017204.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434708"/>
    <dbReference type="CTD" id="794625"/>
    <dbReference type="GeneTree" id="ENSGT00940000166377"/>
    <dbReference type="OMA" id="GIAKHFN"/>
    <dbReference type="OrthoDB" id="9435504at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009125">
        <property type="entry name" value="ATPMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34038">
        <property type="entry name" value="ATP SYNTHASE MEMBRANE SUBUNIT DAPIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34038:SF1">
        <property type="entry name" value="ATP SYNTHASE MEMBRANE SUBUNIT K, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14960">
        <property type="entry name" value="ATP_synth_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01821">
        <property type="entry name" value="DAPIT"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="58" mass="6334" checksum="50A13668EF49D34E" modified="2018-12-05" version="1">MAGHDAGTQHQFTGIAKYFNAYTITGRRNCVLATYASIAAIILFFKLKPKKQKAVTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNP3</accession>
    <name>A0A3B4CNP3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Fraser extracellular matrix complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">FRAS1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FRAS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560623.2">
        <property type="nucleotide sequence ID" value="XM_017705134.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000012960"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036729.2">
        <property type="protein sequence ID" value="ENSPNAP00000012960.2"/>
        <property type="gene ID" value="ENSPNAG00000019185.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431776"/>
    <dbReference type="GeneTree" id="ENSGT00940000163761"/>
    <dbReference type="OrthoDB" id="430044at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071711">
        <property type="term" value="P:basement membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007492">
        <property type="term" value="P:endoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010669">
        <property type="term" value="P:epithelial structure maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033334">
        <property type="term" value="P:fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000005">
        <property type="entry name" value="Extracellular matrix protein FRAS1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000026">
        <property type="entry name" value="Fraser extracellular matrix complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000003">
        <property type="entry name" value="Fraser extracellular matrix complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000006">
        <property type="entry name" value="Fraser extracellular matrix complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000035">
        <property type="entry name" value="Fraser extracellular matrix complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039005">
        <property type="entry name" value="CSPG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051561">
        <property type="entry name" value="FRAS1_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739:SF1">
        <property type="entry name" value="EXTRACELLULAR MATRIX ORGANIZING PROTEIN FRAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16184">
        <property type="entry name" value="Cadherin_3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51854">
        <property type="entry name" value="CSPG"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043545732" description="Fraser extracellular matrix complex subunit 1" evidence="15">
        <location>
            <begin position="24"/>
            <end position="4004"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="3901"/>
            <end position="3922"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="16">
        <location>
            <begin position="23"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="16">
        <location>
            <begin position="90"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="16">
        <location>
            <begin position="154"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="16">
        <location>
            <begin position="216"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="16">
        <location>
            <begin position="280"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="16">
        <location>
            <begin position="370"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="1099"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="1214"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="1324"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="1459"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="1591"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="1707"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="1831"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="1954"/>
            <end position="2054"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="2075"/>
            <end position="2174"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="2182"/>
            <end position="2312"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="2196"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="2308"/>
            <end position="2401"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="13">
        <location>
            <begin position="2436"/>
            <end position="2533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012960.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="4004" mass="442722" checksum="D56B49EFC2411800" modified="2024-11-27" version="2">MALAWIFISVLPLLGTGSQGVQGACVYQGAPVANNTSWKPETCQDCTCHGDVVICKATRCQNPQCDFQRGERLRIPPNKCCPECFPQSAGSCQYRGAVYGHDSQWSESKCSVCTCSLGKATCVPPACPQLSCGSGQSPYIPVGECCPKCTQNGASCSWEGEVRRDGEEWTPSPCTKCKCQNGQTHCSTVECPSITCKLNENLVIQPGRCCPQCVSNPCIAAGKEYKDGHQWQKNSCTTCVCDRGQSRCHIEKCPPLHCDKGQMKVKRPGQCCDECVSTKGSCLYEGAVRYHGDMWNGTGCEFCTCERGQVLCQRAECARLECPRGEEMVYLSGKCCPECKAAVSSCMYTHPEKEGTKTNVKSFRRLANLESVSESPCRECQCQDGHVTCYQSSCPTCPAGTLAVPRQGQCCSECHPAVSCHQDCLTCSSTPDQCGSCKDARAFLWNGHCLHSCPQGFYPHGRVCAACQPSCLTCENGFECTACGGSLLLSGRQCVATCESGMYQDHTQCLSCHDSCLSCGGGGPQDCLLCSDPSLLLKEGFCVADCGPGFYVSQGNCYACDPSCATCHPDNPSCLSCPVGYALHHGKCVSECPAQHYRDSYGRCQMCHSSCASCSGPSASHCTSCPSSLPLYQGQCVESCGEGLFARDGHCYSCHVSCRACVGPESSDCVRCVKPEEVLQLQHSYVSHGACMSTCLPQHFLDTELICRECHASCARCTAGNAHNCTSCLQPSILHHGQCLDRCPHGFHVQDRTCQACHPSCKECSGPSQADCTACPPHASLHNGYCRTSCPEGQYLNAVGYCNDCQPGCQRCTADLQLGSGTVCLWCKGSRMLLLGDHCVEHCPSGHYKYHGACKKCHSSCADCSGEGPQACTSCLTTAVLSPSGMCTTRCPIGYYADESRVCKKCDRQCLSCEMAGVCTSCRDPAKVLLFGECQYESCAQQYYLNTTTRTCRECDWSCNSCRGPLRSDCLQCMEGHVLQNGVCVPSCSPGFYRDGERCLKCDDHCEQCDGPGQCHQCQTPYTALKGQCVLDCGKHYFLVPTKQRCIACPSSCVECVSAGQCRVCAENTFKKNGQCTPDCGHGYYANKKARECQANTHAPSLHINGSHLVPLSGVTPLDPALLSIKDSDSQSESMVLQLVQPPNNGRLVVLEEDKERSLRKDDTFSLAQLRNRAVRFVHTTDQSKFGQFTLRAADPQLFSRPQTVNVQAVSQEPPLVVTNQPIYIEPGETALLTRSSLHIEDPDNPQDVLLMVLDPPHHGRLTRVHADGQVTQFKLEELAREQLQYVHDGTSGAQDRLLLQINDGHSYQNVLLHVNIDQKNMLSPHMRSIPKVWVKEGGMVQLSKKHLKAEYKGASDSEILYSIQSDSGQPQYGEIVFVPMPADGPVEAWQAFPEDRDTTPTTTFTQQDVNEGTVWYRHYGKSTQRDTFQFQVSSEAFPDTRSDAQTFVIGIMPQTPGLPQLTPGADLQITALEDRVTLISPTALSFTDSETPSNKLIFNITKPLPSGQGMLEHGDRPYIHVRFFTQADVDAGKIIYRPPQAPSHLQELYQYSFTGLPDSVNFHFTVSDGEHTTPEMDFSILLLANHQQPPVFQILDPVLEVSLGGRATVGGQQLAVTDADTAPDELEFELVEGPVHGNLLRVDYGTQTQMVNGDTFTFDDLTRNVLQYEHTGLNTEEDTMIFSVTDGMSMTSTTVQVIVSGGGGDGPKRAPDALLSMEVAEKSSTVVRRSHLAYVDNDSSDDKIRIQLVSVPMYGILTKTNSESDHEELNEYSTFTMEDINHHKIRYVTSFETGNQPVTDIFHFIVYDGENNRLENQMCTITITSVHRQPPVVTVRSGIKVQEGGRVQLSANHIIASDPDTPKKDLLVWLISPPKYGFIENTKRVGSMGGARVISPDVPFTMEDLTSDHIFYVQNVHQANIHQDVFSFYISDGSSQTEAFDVTIDILQTKEDKVPVISVNSIHVEENTGVVITNSSLNVLDLDTPENEIVLTVIKKPSYGKLRRRQFYSQPLENGKILLQGSTFTYQDVLDELLVYTPDGQGSATDEIRFSVTDGVHTETGRLEFSMDVRKSEGPRVTINRGLQLAAGSSSKITEQQLKGTDIDSENLKLKFTLTKDVSVGILQLTKGRSKVQLSTRGPVKSFTQEEINKGFVEYIHEKGESGGSFSFKFDLEDPEGNKVIDESFFISVLEDKLPPSVVVNKGLVLDENSVKKISTLQLSSTDQDSEPGELVYRITKQPQLGHLELSTNPGKRISTFTQADLASRNVQYVHTSEEEKHSDEFSFIVSDGANEISQTFYITIRPVDDSLPVLQVPGMRVQEGVRKTITEFELKAVDADTEEDSVTFTIVQAPRHGTIERTSNGQHYRQTNTFTMDDIYQNRISYNHDGSNSLKDRFTFTVSDGTNMFFIIEEGGKEVVTAAPQKFKIDIMPVDDGTPRIVTNLGLQWLEYMDNKATNLITKKELLTIDPDTDDGQLTYEITAKPKHGYLESKLKPGKPITSFTQADINLGLIRYVLNEDKVQETMDNFKFLVKDSKPNIVSDNVFHVQWSLISFEHSSYNVSEKAGTVAVTVKRTGNLNQYAIVLCRTEQGTATSTSTVGSRPGQHDYVEHAGQVQFDEREDTKVCTIVINDDQVFENIESFTVELSMPVYALLGQVTRAKVNINDTDDEPTLQFDKKTYHVNESTGFIFAPIERKGDTSSTVSALCYTVPRSARGSSLHALESGSDYKSRGMSGENRVIFGPGVSMSTCDVKLIDDSEYELAEEFELVLSDASDNARMGDRTVAKVIIDGPNDASTVFLGNGSFTFSEDAGTIEIPVLRQGSDLSSVTSVWCATRPADQDSATPGVDYIPSSKKVEFKPGKTEETCSLTIMDDIQNPTIEGKESFIVFLSSPNGAVLTEPYEASVYITDTAQDIPSMQFEKAAYTVKEKDGVLNIPIVRTGDLTYKSSVRCFTRTMSAMVMDDFDERRNADVSQIVFHKGEKVKNCTVYINDDSVFEPEEEFQVHLGTPQGDHWSGAMIGVNDIVTVTITNDEDAPTIEFEQASYQVREPSGPDGIEVLNIKVIRKGDLDRTSKIRCSTRDGSAQSGVDYNPKSRVLKFSPGVDHILFKVEILSNEEREWHESFSLVLGPDDPVEAVLGEVTMATVTILDQEAAGSLILPAPPIVVSLSDYDYVQEVTKEGSKKSPSPGYPLVCVTPCDPHYPKYSSMRERCEEAGINQTSIHFSWEVAAPTDTSGARSPFETVTDSTPYTTVNHMVLDSIYFSRRFHVRCVAQARDKAGHLGTPLRSNIATISTEGSICHTPVTTGTARGFQAQSFIATLKYLDVKHKEHPNRIHISVQIPHQDGMLPLVSTMPLHNLHFLLSESIYRQQHICSNLVTLKDLQGISETGFLDDVSYDTISLGPGYDRPYQFNPNVRETKTIQLYKHLNLKSCIWTFDAYYDMTELIDVCGGSVTADFQVRDSAQSFLTVQVPLYVSYIYVTAPRGWASLEHHTEMEFSFFYDTVLWRTGIQTDSVLSARLQIIRIFIREDGRLVIEFKTHAKFRGQFVPEHHTLPGHKSHLMAPDHLGGMDFDLQLVWSAQTFDSPYQLWRATSSYSRKDYSGEYTVFLIPCTVQPTQPWIDPGDKPLSCTAHAPEKFLVPIAFQQTNRPVPVVYSLNTEFQLCNNEKVFMMDPATADISMAEMDYKGAFSMGQTLYGRVLWNPDQNLNSAYKLQLEKVYLCTGRDGYVPFFDPTGTLYNEGPQYGCIQPNKHLKHRFLLLDRKQPDVCDRYFHDVPFEASFASDIPELQSMAAMPGVDGFTMKVDALYKVEAGHQWYLQVIYVIGPESISSPRIQRSLTYELKRGRRDLVDRSGRLTLDESLIYDNEGDQVKNGTNMKTLRLEVEASATFNPQMGGSVGGGVAAFILLLLVLLAFCLLFRKCRKSAENKRPTKAGEEYPLNTKVEVCLERLEKNLNSKHCTVRNVNLLNKSAESCKVNGVKVKQVNLQVKLHNNLNDGTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CP35</accession>
    <name>A0A3B4CP35_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like modifier-activating enzyme 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">UFM1-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">UBA5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538894.1">
        <property type="nucleotide sequence ID" value="XM_017683405.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CP35"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013115"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020608.2">
        <property type="protein sequence ID" value="ENSPNAP00000013115.1"/>
        <property type="gene ID" value="ENSPNAG00000018983.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411700"/>
    <dbReference type="CTD" id="79876"/>
    <dbReference type="GeneTree" id="ENSGT00940000156177"/>
    <dbReference type="OMA" id="MNIVKDY"/>
    <dbReference type="OrthoDB" id="206053at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071566">
        <property type="term" value="F:UFM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071569">
        <property type="term" value="P:protein ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000066">
        <property type="entry name" value="Putative ubiquitin-like modifier-activating enzyme 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF9">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="10">
        <location>
            <begin position="50"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="400" mass="44312" checksum="BD1BEFDD6131B6D7" modified="2018-12-05" version="1">MATAEELKRRVRELENELIKCKQRRSSEEQVHARAKIEKMSAEVVDSNPYSRLMALKRMGIVDNYEKIRSFAVAVVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVEAAENTLRNINPDVVFETHNYNITTMDSFTHFMDRISNGGLEEGKPVDLVLSCVDNFEARMAINTACNELGQVWMESGVSENAVSGHIQLIIPGETACFACAPPLVVAASIDEKTLKKDGVCAASLPTTMGVVAGILVQNVLKYLLGFGSVSYYLGYNAMQDFFPTMAMKANPQCDDQHCRRQQEEYKSKVTEHPKQEAVEEEVVVVVHEENEWGIEVVSEVTEAELQAASGPEPDLPEGITVAYTIPEKEETSGQTVEETDQSLEDLMAQMRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQ82</accession>
    <name>A0A3B4CQ82_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037400872.1">
        <property type="nucleotide sequence ID" value="XM_037544975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQ82"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021151.2">
        <property type="protein sequence ID" value="ENSPNAP00000013536.2"/>
        <property type="gene ID" value="ENSPNAG00000019399.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000021180.2">
        <property type="protein sequence ID" value="ENSPNAP00000013518.2"/>
        <property type="gene ID" value="ENSPNAG00000019399.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440104"/>
    <dbReference type="GeneTree" id="ENSGT00600000085600"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF143">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 4A-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013518.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="217" mass="23326" checksum="D7E80F7CF38B7479" modified="2024-11-27" version="2">MTGDSDMEIVDSADHNAASQNNNAPNEIGALVEVTFQKNPHQEQKYLEAEPKALGLTQIMLSLFVISISIVASKRNVYIMVPYGITCSLSTLGIIAGSVAIAAQNLHLPKLKACLVMQILTCAAAGVCFIINLVVTVDMGLVYGCWQYNHENTTLHQDLCERMLEAHSHLVGLKMLCQAAQVAISATLAAFCCKVIQCCSPRASMPMIVVNAPTAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSF0</accession>
    <name>A0A3B4CSF0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CSF0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015027"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023100.2">
        <property type="protein sequence ID" value="ENSPNAP00000015027.2"/>
        <property type="gene ID" value="ENSPNAG00000021015.2"/>
    </dbReference>
    <dbReference type="OMA" id="HCGHSKG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015027.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="120" mass="14522" checksum="590BA5AFAD786F3E" modified="2024-11-27" version="2">VCTRSCLFMLPIWEKALPHTLQLKGRSPVWMRMWHRSISGPANVLWQRVHGICMRGFSWLRTCLFKSNMYTKDLPHIEHKYLRSPMKLLPHTLHWYARLHDFWLFQILQLSCFLFTIVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CU76</accession>
    <name>A0A3B4CU76_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KLHL22</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017551872.1">
        <property type="nucleotide sequence ID" value="XM_017696383.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392136.1">
        <property type="nucleotide sequence ID" value="XM_037536239.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392137.1">
        <property type="nucleotide sequence ID" value="XM_037536240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CU76"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014079"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021878.2">
        <property type="protein sequence ID" value="ENSPNAP00000014079.1"/>
        <property type="gene ID" value="ENSPNAG00000019987.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426704"/>
    <dbReference type="CTD" id="84861"/>
    <dbReference type="GeneTree" id="ENSGT00940000159598"/>
    <dbReference type="OMA" id="ACYKPST"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005827">
        <property type="term" value="C:polar microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF5">
        <property type="entry name" value="KELCH-LIKE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="50"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="639" mass="72069" checksum="413467ABE8386613" modified="2018-12-05" version="1">MADDMDTGPVRVGRGVRSGQPCARQRFCSRGHSRELLDGLLALREGGVLFDVVLLVEERPIQAHRILLAASCGYFRGMFAGGLREMQQTEITIHGVTHTAMTKLLDFIYTSELELDLDTVQEVLCAATLLQVKDVIGFCCEFLFSWLDDDNILEVEKLADIYGLEQLGENVRLYLLKNIQTFSRTSVYRQLPAEKVLRVLSSDELQVSSEYEVFEAALYYYYSPKELDMDQMCLQTGGTRAETFLEVPLLMLEAVRFCLMEKATLQRLHSRLKPCPLRESVSAALRYHSQELWQPVMQSPLTQTRSSSLCILGFGGMYSSGPPADNEERVQVFHQSWGEWRSLGADRTPRMSNMGIAVLNNFVYLVGGDKNSSGFRAEARCWRYDPRHNTWCNVEPLRQQHADHCVCVVGDYIYAIGGRDYSNELDCVERYDPRTNTWEYVAPLKREVYAHAGAVVDGKIYIACGRRGMAYLKETYCYDPQDNRWTVCSEGPVERAWHGMVALNGRAYVIGGSNDGCGYRRDVLKVACYNPTADVWSVVCPLPAGHGEPGMAVLDGRIYILGGRSHDKGSRMKYVHIYNPEEDHWQNGSSLDDRVSGLSACVALVPRNTLAQAQSWEQRAKASWEEVDWDDSDNSSEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVC6</accession>
    <name>A0A3B4CVC6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phenylethanolamine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PNMT</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVC6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015298"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037668.2">
        <property type="protein sequence ID" value="ENSPNAP00000015298.2"/>
        <property type="gene ID" value="ENSPNAG00000003679.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011708"/>
    <dbReference type="OMA" id="NNYMPPR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004603">
        <property type="term" value="F:phenylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000065">
        <property type="entry name" value="Phenylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000940">
        <property type="entry name" value="NNMT_TEMT_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10867">
        <property type="entry name" value="NNMT/PNMT/TEMT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10867:SF18">
        <property type="entry name" value="PHENYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01234">
        <property type="entry name" value="NNMT_PNMT_TEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51681">
        <property type="entry name" value="SAM_MT_NNMT_PNMT_TEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015298.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="29078" checksum="312BCA8B78C5F91A" modified="2024-11-27" version="2">MEGEDRGEKAVAAMEACYQGFDPAAYLQYNYTPPRADFTRSDSIVPWKLKCLHEAFSEGEVLVDVGSGPTLYQVMSGCEHFDRVVLSDFLEVNRKELRIWLQDGKSSLDWTPYLKFVCELEGRKCNKNERLRSVVTDVLPINIHQPCPLPSGSLPSTGADCLVSSFCLESVSPDLSSFTQALGHLSGLLRSGGHLLLIGALGESFYLGAPGLRIPVVPLDEAQVCSSLRASGFHLLQLSVYHLPPDMRVGVDDVTGVFFAKARKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CW45</accession>
    <name>A0A3B4CW45_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,3-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.222</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LFNG</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>3-O-(alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70511"/>
            <dbReference type="Rhea" id="RHEA-COMP:17919"/>
            <dbReference type="Rhea" id="RHEA-COMP:17920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="ChEBI" id="CHEBI:189633"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>3-O-(alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70531"/>
            <dbReference type="Rhea" id="RHEA-COMP:17922"/>
            <dbReference type="Rhea" id="RHEA-COMP:17923"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="ChEBI" id="CHEBI:189634"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18 21">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.222" evidence="18"/>
    <dbReference type="RefSeq" id="XP_017558695.1">
        <property type="nucleotide sequence ID" value="XM_017703206.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CW45"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015563"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023788.2">
        <property type="protein sequence ID" value="ENSPNAP00000015563.1"/>
        <property type="gene ID" value="ENSPNAG00000031265.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430609"/>
    <dbReference type="CTD" id="3955"/>
    <dbReference type="GeneTree" id="ENSGT00940000158717"/>
    <dbReference type="OMA" id="CPHTAVF"/>
    <dbReference type="OrthoDB" id="8959630at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033829">
        <property type="term" value="F:O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055016">
        <property type="term" value="P:hypochord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045665">
        <property type="term" value="P:negative regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000003">
        <property type="entry name" value="Beta-1,3-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017374">
        <property type="entry name" value="Fringe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038073">
        <property type="entry name" value="B-acetylgalactosaminyltfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 18 21" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="23">
        <location>
            <begin position="104"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR038073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038073-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR038073-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR038073-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="375" mass="41764" checksum="1096DBBFD0C18622" modified="2018-12-05" version="1">MLKSHSRKTLLSAAGASVTCLALLLLLAQRQRSATEGARREREVGLRSLQSLGEAGTEDGIQSEQAGGKRGFSAYFSKLTRNRREAEKPGGAAGATSSPPPAENISADDIFIAVKTTKKFHKSRLELLLDTWISRNVQQTYIFTDGEDEELKKKMGSHVINTNCSAAHSRQALSCKMAVEYDKFIESGNKWFCHVDDDNYVNMKTLVQLLSQYPHTQDMYIGKPSLDRPIEATERLGDNKMRPVNFWFATGGAGFCVSRGLALKMSPWASGGHFMNTAEKIRLPDDCTIGYIIESVLGVPLTRSNLFHSHLENLQQVSRSEVHKQITLSYGMFENKRNIINMKGAFSVEEDPSRFKSVHCLLNPDTPWCPPQVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWF0</accession>
    <name>A0A3B4CWF0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Eukaryotic translation initiation factor 4E type 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Eukaryotic translation initiation factor 4E-like 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">eIF4E-like protein 4E-LP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">EIF4E2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation. In association with GIGYF2, assists ribosome-associated quality control (RQC) by sequestering the mRNA cap, blocking ribosome initiation and decreasing the translational load on problematic messages. Part of a pathway that works in parallel to RQC-mediated degradation of the stalled nascent polypeptide. GIGYF2 and EIF4E2 work downstream and independently of ZNF598, which seems to work as a scaffold that can recruit them to faulty mRNA even if alternative recruitment mechanisms may exist.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation. In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. In P-bodies, component of a complex that promotes miRNA-mediated translational repression. Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3. Does not interact with eIF4G (EIF4G1, EIF4G2 or EIF4G3). Component of the 4EHP-GYF2 complex, at least composed of EIF4E2, GIGYF2 and ZNF598. Interacts with GIGYF2 (via the 4EHP-binding motif); the interaction is direct. Interacts with EIF4ENIF1/4E-T (via YXXXXLphi motif); increasing affinity for the 7-methylguanosine-containing mRNA cap.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554663.1">
        <property type="nucleotide sequence ID" value="XM_017699174.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWF0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015695"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023988.2">
        <property type="protein sequence ID" value="ENSPNAP00000015695.2"/>
        <property type="gene ID" value="ENSPNAG00000007063.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428289"/>
    <dbReference type="CTD" id="9470"/>
    <dbReference type="GeneTree" id="ENSGT00940000154694"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E type 2 isoformX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF17">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 19" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 19" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015695.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="236" mass="27045" checksum="6F231BC1FFC3A8CB" modified="2024-11-27" version="2">MNNKFDALKDDDSGDHDQDNGSPKDSEKEKTEDEEKDQNTTKRKPVVPGAGEHPLQYNYTFWYSRRTPGRPASTQSYEQNIKQIGSFASVEQFWRFYSHMIRPGDLTGHSDFHLFKEGIKPMWEDDANKSGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKAWEDFHGLVNASGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ26</accession>
    <name>A0A3B4CZ26_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ26"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015804"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024104.2">
        <property type="protein sequence ID" value="ENSPNAP00000015804.2"/>
        <property type="gene ID" value="ENSPNAG00000035785.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162676"/>
    <dbReference type="OMA" id="ESQACPG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="20"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015804.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="153" mass="17251" checksum="288D18BB05E0E5F8" modified="2024-11-27" version="2">SMRHWQLNTSVLQTAVWWSVSPGESVTLQCTVLSEIRAADLRVLWFRAAAGQSFPEIIYTHQNSNSRQCEISSSTSCSLYEFSKNILDHNHTGTYYCAVAVCGKIIFGNGTSVELSKCSCVLLGTCSIFVLFIDGVKWFIVEKLFMQIYLQWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5G0</accession>
    <name>A0A3B4D5G0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="11">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PIM2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5G0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018351"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027520.2">
        <property type="protein sequence ID" value="ENSPNAP00000018351.1"/>
        <property type="gene ID" value="ENSPNAG00000003844.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163427"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000363">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000708">
        <property type="entry name" value="serine/threonine-protein kinase par-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017348">
        <property type="entry name" value="PIM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051138">
        <property type="entry name" value="PIM_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984:SF23">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PIM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037993">
        <property type="entry name" value="STPK_Pim-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017435607" description="Serine/threonine-protein kinase" evidence="16">
        <location>
            <begin position="20"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="58"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="64"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037993-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037993-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="338" mass="38076" checksum="6D19997B8E65DE86" modified="2018-12-05" version="1">MHCWCVSFCKLCRLGCFSCFPFLKPKTGVLVYGSWTVPVLQETLFTFVPAKEPFDKQYKCGALLGSGGFGSVFAGQRISDGLQVAIKQVSHDRVQQWARLPDEPSLVPMEIALLVQLGSGSGAGPSHRGIIRMLDWFEVPGQGFLIVFERPQPCQDLFDFITERGAVEEPIAQRFLKQVIEAIQFCHSKGVVHRDIKDENILVDTRTGDIKIIDFGSGALMKDSIYTDFEGTRVYSPPEWILQQRYEAIPLTVWSLGVLLFDMACGDIPFEQDRDIVQANPSFTKRVSKECQSLIRWCLAYHPKDRPTLEQILLHPWMTESAEDIGDLQEESNIAPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7R7</accession>
    <name>A0A3B4D7R7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Intraflagellar transport protein 56</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Tetratricopeptide repeat protein 26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TTC26</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT56 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577586.1">
        <property type="nucleotide sequence ID" value="XM_017722097.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017577587.1">
        <property type="nucleotide sequence ID" value="XM_017722098.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7R7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039580.2">
        <property type="protein sequence ID" value="ENSPNAP00000019560.1"/>
        <property type="gene ID" value="ENSPNAG00000036067.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000077631.1">
        <property type="protein sequence ID" value="ENSPNAP00000065407.1"/>
        <property type="gene ID" value="ENSPNAG00000036067.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108442178"/>
    <dbReference type="CTD" id="79989"/>
    <dbReference type="GeneTree" id="ENSGT00390000000159"/>
    <dbReference type="OrthoDB" id="95390at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120170">
        <property type="term" value="F:intraciliary transport particle B binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035720">
        <property type="term" value="P:intraciliary anterograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008594">
        <property type="term" value="P:photoreceptor cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035845">
        <property type="term" value="P:photoreceptor cell outer segment organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039023">
        <property type="term" value="P:pronephric duct morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000588">
        <property type="entry name" value="Intraflagellar transport protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001530">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000271">
        <property type="entry name" value="Tetratricopeptide repeat domain 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030511">
        <property type="entry name" value="TTC26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14781">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14781:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="557" mass="63924" checksum="B8314B5F29148D85" modified="2018-12-05" version="1">MLLSRVKPAVGGDTALSSSEKKRKNKSRKTPRLEDYLSQRDYLGALTLLEFQRNGGAAEEHTDQWIGYCAFHLGDHKRAMEEYKSLTLKPDCPAEVWVHLGCSLFFLGLYKEAEEVALKGPKSQLQNRLLFHLAHKFGDEKKLMGFHQNLEDVTEDQLSLASIHYMRSHYQEAIDIYKRILLQNREFLALNVYVALCYYKLDYYDVSQEVLAVYLQNVPDSAIALNLKACNHFRLYNGKAAEAELKNLIDISSSSFEFAKELIRHNLVVFRGGEGALQVLPPLIDVISEARLNLVIYYLRQDDVQEAYNLIKDLEPTTPQEYILKGVVNAALGQEIGSRDHLKIAQQFFQLVGGSASECDTIPGRQCMASCFFLLKQFEDVLIYLNSVKSYFYTDDTFNFNYAQAKAALGSYKEAEEVFLLIQSEKIKNDYVYLSWLARCYIMNQKARLAWELYLKMETSSDSFSLLQLIANDCYKMGQFYYAAKAFDVLERLDSNPEYWEGKRGACVGIFQLILAGREPRESLKEVLPMLRSTGNPQVEYIIRIMKKWAKDNRVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8U0</accession>
    <name>A0A3B4D8U0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8U0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029972.2">
        <property type="protein sequence ID" value="ENSPNAP00000019853.2"/>
        <property type="gene ID" value="ENSPNAG00000026594.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164468"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF296">
        <property type="entry name" value="PROTOCADHERIN 1 GAMMA 11 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="697"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="822"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="38"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="143"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="252"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="358"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="463"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="587"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019853.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="849" mass="94347" checksum="FEF456C621E0AECD" modified="2024-11-27" version="2">MDVLCSHIARNTGSISGVVRRLTRFLVLLVFVLTVARGQVRYSIPEEMNKGSVVGNIVQDLGLDVKRMKSGRARIFTEDSREYIGLNVDKGTLVVRERIDREELCAQVTPCSLHFQIILENPMELHRIDVEILDINDHAPVFARKEIHFDIRESAVPGARFSLDNARDPDVGLNALQRYTLNPTDHFTLKELSRNDGTKYVEMVLQTPLDREQQEEHKLILTAFDGGSPQKSGTVRITVVVIDANDNHPVFSQPVYRVSLPENTEKGRTLVIVSAADKDKGSNGEVTYSFSQSTGIEALNLFCIDSNTGEIKVNALLDYEKSKQYELNIEAIDNGGLTDTSKVLVEITDVNDNAPVISVISFSNPIPEDSAPETVIAMLNIKDIDSGKNGQVKCSVNSDLPFRIKSSSSNFYSLVTDQFLDREKVSEYNITIRATDEGSPSFSTNKTLQLKISDVNDNAPVFQRQFYTAYVMENNTPGVSIFAVTASDKDSGNNARISYFLEDVLVNGVSASTYISVHTESGEILAVRSFDYEQTKEFNIRVKAQDGGNPPLSSNVSVKIIIQDQNDNAPQVLYPVQTGGSVVAEMVPRSADVGYLVSKVVAVDVDSGQNAWLSYKLQKATDRALFEVGAQNGEIRTVRQVTDKDAVKQKLTVVVEDNGQPSRSATVNINVAVADSFPEVLSEFTDFTHIKDYNDNLTFYLVLALAVVSFLFIVSIIAILSVKCYRWRRERMFYKSGANLPVIPYYPPLYADVGGTGTLQHVYNYEVCRTTDSRKSDLKYARPSSESIISLDTSGTQTLTHVQKQKLNHDSEDQRFSRNRNWIVQFASWTSFVLISPGTQALSLVLFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAW0</accession>
    <name>A0A3B4DAW0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DAW0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020588"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031291.2">
        <property type="protein sequence ID" value="ENSPNAP00000020588.2"/>
        <property type="gene ID" value="ENSPNAG00000031995.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00670000098179"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF2">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="45"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020588.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="181" mass="20420" checksum="236E2DA068F6B2AB" modified="2024-11-27" version="2">SVVHWKYDGVDIYFLSFLECLLLLWFICCLYNFLMQLIFEKVSQTAASDVVQQVSYAVEKEGGGFALSMHAKDFSPEEMSIKQVGRTLHVCGKTEKREEDGEGSYSHRVQEFRREFLLPEGVNPEELSCSLAEGKLFIQTPKTQQSEKPDRLTLPLRHSANDTLSGTRNPGPKSLAFVLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBC2</accession>
    <name>A0A3B4DBC2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Growth regulating estrogen receptor binding 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RUSC1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GREB1 family.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000020381"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039997.2">
        <property type="protein sequence ID" value="ENSPNAP00000020381.2"/>
        <property type="gene ID" value="ENSPNAG00000027200.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008041"/>
    <dbReference type="OMA" id="SEYSVEW"/>
    <dbReference type="OrthoDB" id="9989163at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060026">
        <property type="term" value="P:convergent extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021983">
        <property type="term" value="P:pituitary gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028422">
        <property type="entry name" value="GREB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048659">
        <property type="entry name" value="GREB1-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046927">
        <property type="entry name" value="GREB1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048657">
        <property type="entry name" value="GREB1-like_cpSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046926">
        <property type="entry name" value="GREB1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049100">
        <property type="entry name" value="TAGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15720">
        <property type="entry name" value="GREB1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15720:SF13">
        <property type="entry name" value="PROTEIN GREB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20692">
        <property type="entry name" value="cpSF2-GREB1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20688">
        <property type="entry name" value="GREB1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20267">
        <property type="entry name" value="GREB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15782">
        <property type="entry name" value="GREB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20691">
        <property type="entry name" value="TAGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GREB1 N-terminal" evidence="7">
        <location>
            <begin position="223"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like second" evidence="9">
        <location>
            <begin position="462"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like circularly permuted SF2 helicase" evidence="11">
        <location>
            <begin position="807"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like circularly permuted SF2 helicase" evidence="11">
        <location>
            <begin position="1291"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="domain" description="TET-Associated Glycosyltransferase" evidence="10">
        <location>
            <begin position="1608"/>
            <end position="1830"/>
        </location>
    </feature>
    <feature type="domain" description="GREB1-like C-terminal" evidence="8">
        <location>
            <begin position="1847"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="426"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1226"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1226"/>
            <end position="1235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020381.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2009" mass="223349" checksum="D95E4E5D7D6A8C39" modified="2024-11-27" version="2">MLVFRLGFRLVVRPGFRLVVRPGFRLVVRPGFSGWCPGRGSGWWSDQGSGWCSGWGSGWWSGRGVQVGGQSLPSDKVSSGLFLEVTRVHTSCVWRFSGGRDLLSWGSGGGGWGWAGMGVPLTLTLRPKVDESERVEAPSRRCELQPSGAPAVNLPGAAERVFGDSEEMGNSYAGQLRTTRFEEVLHSSIEASLRSNTVVPRPVFSQLYLEPEQRPLSRDGHMENEEEEDEDDSESNSPPIPYQMKPPPEGSCITDGFCQAGKDLRLASIATECLDVPPGFLLVGAKSPSIPDHILVCAVDRRFLPDERGRNALLGFLGNCMGCGEKSFRYFTEFSNHINLKLTTQPKKQKHLKYRLLRNAQGVLVKGPPICWKGIDSRVRQTTSNMSGGLEEHAPNPGANDLLPSSGAAGLNTASYTDLNTHLQASETSPHMTNGSHVPAPQQALPLTGLTAHGPGRPTVAASMANSGPPKKRHKGWSPESSAPTEAVNRPASSNTSNGTRSADTVASGFVSQGSSPPLPPGESVTVPDGLLQTCGLQPVIFKGHGALPRLCGSTGDVLVSGRLQACYCSSQTLPRVYEQYGTSSVQPLSTEMQVLLTVYYLVQLAPDQMPLMEDLEQIFMRSWRESHLSEIRQYQQSHPQFPTPLPGLAQPLTPSQLPWLAKLATSSSGESVLVLDMAPSLAAALDGTFQKLMEGKLSQTRYVVIICMGRSQETESCIVVTGKHQSRALAESMLSPSDCMSKITQELSSGMTAELEAYFSSLGLDGDVEALLEKASAEKSSASAQGCMQEQSGEPTCTEYRVEWREIRPIQLAVARKLLSHVCAIADSSTHNLDLGSFDKVEFLICVPPSEVAFQQVVLHLWNSGVLQELGLDQDCLSPKDAEQYVVKLDQAAVAKISGLIQKSKLNPYTLFILVHDHAHWDISSEQYSRVGGEPHSGLVDSLLNCRQIQEAANILTLHVTSFPFVLQTQFTRISPYNEINWPSAFNNDVDLYQDKTKYFGVSELLESTRSGNGLPLLRFDSSFESMVAALEERFPRLHSVVIRTQVLLQHYSVALMATAKGQAQLQKHTSVETLEMVQCLLGSAHRCPNHHGHMLLLRIPFPALAALAHQRLCHVRDRLGLHERFEVVLGDPTSGIVVGKHFLNQLRVWLKIQDPEWTPHTYLELEALPCILIFTGMNPMGESLPRSLKYCDQRVMSCCALQRTALEQELGLAAYLVSAELQSQQGGRTSSDLSENDPDKLSGTDHEEDELVVDVNSTLLGADHSRTDSSPHKQTQPVLSGDPPHGQRRSSKSTSPGSSTQAHASPLRPFCSWARNLNRPPVVLLPKALYDLITTIDASGLPKPTSFLPHASVEWVSSFRPLLSKMMTCTEQSLYYRQWTVPRPNHMDSSNRAEGRTDNFHPRRLILSGPPQVGKTGAYLQFLGILSRMLIRLMEVDIYDEEDINCNFQSDLVLYHQAIASWPNADTMRGIPFDFTIHDPKYEDISTIYSPDFTPDGEKNARRQEDVFLRRRTARIKLSKYAAYNTYHHCEQCHQYMGFNPRYQLYESTLHAFTFSHLLLGEEIQLYFIIPKSKEHHFSFSQPGGQLESMRLPLTSDWNPDCIKSPIFTPTTGRHEHGLFNLYHAMDGASHLHILVIKEYEMAVYKKYWPNHIMLVLPTIFNGAGIGAAHFLIKELSYHNLELERNRRVEHGSRPQDVWPFIILADDSCVMWNSVDVDAKSGIAGPERNVSLKQVLQHMESSPDITQYGLCGLRKWSSRGSRTGQRREPFSRGHLHDFLLLNVDLTHDIQYNQNRFTCDDVDFNLRIQSAGLLVCRFNNFSVMKKQIAIGGYRTFIIKTKMTDVPTTVQPSQYICAPDSKHLFLATPAQLLLEKYLQHMSQRLFPLSAGNYSHPVLSVDCYLNLGPEVTVCFVSSRLNSINISTAGLLFGGLLLYFCDSFVTPGFLKKFQFLKGATLCVICPNRSALRQTVVRLELEEEWRFRLSDEFQTANAKEDQPLFFLTGKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBF6</accession>
    <name>A0A3B4DBF6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBF6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020800"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031627.2">
        <property type="protein sequence ID" value="ENSPNAP00000020800.1"/>
        <property type="gene ID" value="ENSPNAG00000027735.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433907"/>
    <dbReference type="CTD" id="777792"/>
    <dbReference type="GeneTree" id="ENSGT00940000166159"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033574">
        <property type="term" value="P:response to testosterone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="108"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="358" mass="41072" checksum="413D70A3FA026AAF" modified="2018-12-05" version="1">MGSIAALEEAKASGFNPMVSVILSRQGSSYSVNADEEEKASLCIVRRAGFLSPTREHTHHLRQKLSALCAVRMATQDLYLFFHGLPLPKIVHSEESLEDFRKFKVQNDDIFVISYPKSGTTWMQEIIPLLLNGGDLTPVESIPNWERVPWLEETRAVLFLDKLSAPRTMVSHMPYPLMPPTFSSSKAKAIYIARNPKDVLVSSFYFHQMASFLDDPGTFEEFMDKFLAGQVLFGKWTDHVKSWRNTDLGDRIFYITYEELIQDLRGALERMLRFLGRQMSEEALDRVTEHCLFKNMKKNNMSNYSLVPQEVMDSSKSHFLRKGIAGDWKNHFSPELEDKFNAVIQKELEGSGINFPWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBR8</accession>
    <name>A0A3B4DBR8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit 37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Leucine-rich repeat-containing protein 68</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PPP1R37</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP1R37 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557451.1">
        <property type="nucleotide sequence ID" value="XM_017701962.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBR8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021857"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033563.2">
        <property type="protein sequence ID" value="ENSPNAP00000021857.1"/>
        <property type="gene ID" value="ENSPNAG00000029048.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429893"/>
    <dbReference type="CTD" id="284352"/>
    <dbReference type="GeneTree" id="ENSGT00940000157454"/>
    <dbReference type="OMA" id="AESTNVR"/>
    <dbReference type="OrthoDB" id="10034042at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000324">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF9">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="624"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="734"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="673"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="690"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="748"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="802"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="864"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="876"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="887"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="908"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="932"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1151" mass="124615" checksum="4A53682E270402D6" modified="2018-12-05" version="1">MNSEDQALDLCNANTKTGIDASAHVSEEIIPRNSLHLMDRDAGKQAGDAETLTDGALARVMDCHVRDEDDNGEIGKEELFPQNNNGLAVDCRPNGSEQDSSREKLQFSVADDGDMDIGVDLSLDESGVLESEPATACGTALSEESASGCTGAKPLQLDTDANKDVQKPSEGGDGMPPGLMSVEVLTPSPAQEEGVGHKHGAKRVTFPSDEEIVSGSVEPKDPWRHAQNVTVEEILNAYRQACQKLNCKPIPKVLKQIQDLTELTQRNECLDLKGEKLDYKACESLEEIFKRVQFKVVDLEQTNLDEDGASALFDMIEYYESATHLNISFNKHIGTRGWQAAAHMMRKTSSLQYLDARNTPLLDHSAPFVARALRISGSLAVLHLENAGLSGRPLMLLATALKMNMNLRELYLADNKLNGLQDSAQLGNLLKFNYNIQILDLRNNHILDSGLAYVCEGLKEQRKGLVTLVLWNNQLTHNGMGYLAAALPCTQSLETLNLGHNAISNEGVHKLKDGLIANRSVLRLGLASTKLSCEGAVAVAEFIAESPRLLRLDLRENEIKTGGLMALSLALKVNTSLLRLDLDREPKKETVKSFIETQRALLAEIQNGCKRNFILAKEKEESQQLMRQSASMAEIATEEPPPEEEEEDPTASGDQGEREKTVPEGDEDEDDGEKSSKSQEDEAHPAATQDDSDSDTDDDEEEVDAEVVKATVQTSPLKQTPPPVLAVTTALCASQTSSVSRPAPCPTPTASESPTVVSSITVTEASVPAGAPPSPGRCISVSSPGRGHKIFMVTRVESPPEQQQSLLALAKSLQERTQETKTAAPKLPELPSPKAPSEPSAPQFEEKHSSAEKAASTQQTNEKTSQSPSEPSQPRETPPAEPVPQPQLQEAQVLLQTTTEQQSSTVSNMELANTQLEQHQRVAASEDAAETLTENDTQSQQTSIQGATQELLSEGLLENNVSPSLVEPVTNNELPNELTEPVMSQKEKQILKPAAESDLHDENIKKHAQMGQLVDFEMDTVVSLEQAISRAQPQTATANSVSCTPSQTVAEGLALEHPPVDQDCITSAGTEDLADSPDESFIEEAECISAETATLPNGLKPEFAFHLLEPETPKAACCVMEHMSVTAELSCGQDLEELLLEASLDTSRDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBX4</accession>
    <name>A0A3B4DBX4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GTPase IMAP family member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Immune-associated nucleotide-binding protein 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Exerts an anti-apoptotic effect in the immune system and is involved in responses to infections.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBX4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020576"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031265.2">
        <property type="protein sequence ID" value="ENSPNAP00000020576.2"/>
        <property type="gene ID" value="ENSPNAG00000005038.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282522"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000536">
        <property type="entry name" value="GTPase IMAP family member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF188">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="18">
        <location>
            <begin position="62"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="261"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020576.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="348" mass="41080" checksum="13CE5C79749070D8" modified="2024-11-27" version="2">MLTSSANLHELLTILLNVWRSKDPFQLAKFNSMLIWLASGAFLFFILYITWASVTPKDLQIGPEVRLMMLGTSGAGKSATGNTILGRNAFRAEASPVSVTRTCQRWTEVVRGRKITVIDTPSIMEMWQSSSQESECVQMASPGPHVFLLVIRVGRFTEEERKTVAWIKENLQEEALMSTMILFTGGDLLEGKPMEMFLNESVELQKLLRLCGGRYHIMNNRETRDFKQVSELLNKIDKMTHDNKSIFNSSRISLKEWRSSRLKEEERCVEKERKIREEEERKRGEIRKGIREMEKKRREMKVWDVREEEEWKRLIREREIRKEEEAKRRVIEEELEIYKKKNWWNVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEC6</accession>
    <name>A0A3B4DEC6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ANGPTL4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEC6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021294"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032450.2">
        <property type="protein sequence ID" value="ENSPNAP00000021294.1"/>
        <property type="gene ID" value="ENSPNAG00000028350.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159478"/>
    <dbReference type="OMA" id="MATGFPF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000019">
        <property type="entry name" value="Angiopoietin-like 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017314879" description="Fibrinogen C-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="10">
        <location>
            <begin position="227"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="88"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="453" mass="52514" checksum="4F92C804828420CB" modified="2018-12-05" version="1">LKMKLLNLLFVFIFICSVISFPYERKSERKEKRVQYAAWDDVNVLAHGLLQLGQGLKEHVDKTKGQMRDVSVKLKVFNSSVTELSRLSQHLQEDSEFLKMKAQSLEEREGQILNISSDMQEKAEELLRDRQKFHEKLNKLEEKVDGMMQGEGLNMAGGNHSDTQIIQWMLEAQNQRIDELVERIKQQQDKLDKQNIRLRTLQSQIQMRSERQKEDETQQNENVEQRDSPVALSSDCHDLFLRGEQASGVYTVQPADSLPFEVSCEMTSEGGWTVIQRRQDGSVDFDQLWDAYQSGFGSLDSEFWLGLEKIYQLTKDKDYVLKIQMSDWRDDHQTVKYHFRLNGVESSYSLRILAPSAGHLESALPSESSGLAFSTRDKDNDQKNDLNCAKHLSGGWWFSNCGRSNLNGRYFVTAPPKQRHQRKQGVFWKTWRGRYYPLKSTIMMIAPAEIENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHG7</accession>
    <name>A0A3B4DHG7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017564163.1">
        <property type="nucleotide sequence ID" value="XM_017708674.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHG7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034526.2">
        <property type="protein sequence ID" value="ENSPNAP00000022364.1"/>
        <property type="gene ID" value="ENSPNAG00000005071.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433842"/>
    <dbReference type="CTD" id="555635"/>
    <dbReference type="GeneTree" id="ENSGT00390000004923"/>
    <dbReference type="OMA" id="TWEIFEG"/>
    <dbReference type="OrthoDB" id="5843172at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000117">
        <property type="entry name" value="Fibronectin type III domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032073">
        <property type="entry name" value="FNDC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052120">
        <property type="entry name" value="FNDC_type_III_4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470:SF1">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16066">
        <property type="entry name" value="DUF4808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017234654" description="Fibronectin type-III domain-containing protein" evidence="10">
        <location>
            <begin position="24"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="28"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="175"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="204" mass="22143" checksum="1B4021B77740EE33" modified="2018-12-05" version="1">MGGKGFAATLLFLGCVSVSSVDADSLSAPLNVTIKTLEGNSAVVTWEILEGDPVIGFAITQQKKDVRMMRFIQEVNTTTRSCALWDLEADTEYIVHVQSISMSGTSPPSDPVLFRTPKESEKLASKSPDEVTMKEVGQAAQLRAGELIIIVVVLIMWAGVIALFCRQYDIIKDNEPNNNKDKAKNSSECSTPEHPTGGLLRSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DIJ1</accession>
    <name>A0A3B4DIJ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 250</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017558356.1">
        <property type="nucleotide sequence ID" value="XM_017702867.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DIJ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022926"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013936.2">
        <property type="protein sequence ID" value="ENSPNAP00000022926.1"/>
        <property type="gene ID" value="ENSPNAG00000000220.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430402"/>
    <dbReference type="CTD" id="90120"/>
    <dbReference type="GeneTree" id="ENSGT00560000078707"/>
    <dbReference type="OMA" id="HVTMFLV"/>
    <dbReference type="OrthoDB" id="10013139at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041156">
        <property type="entry name" value="TM250"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48431">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48431:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17685">
        <property type="entry name" value="TM250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16270" checksum="D490467350E62B4F" modified="2018-12-05" version="1">MPVIPIPRRVRSFHGPHTTCMHSACGPARTSQLVRTKYNNFDLYLKSRWMYSFVRFLLYFGCSLFTSLLWVALSALLCLQYVSVRIFLRLQYKLSVILLLLGHRRLDFGVLNELFIYSMHVTMFLVGGLGWCFMVFVDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DK59</accession>
    <name>A0A3B4DK59_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanylate cyclase soluble subunit alpha-1</fullName>
            <ecNumber evidence="3">4.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Soluble guanylate cyclase large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GUCY1A1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017564868.1">
        <property type="nucleotide sequence ID" value="XM_017709379.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DK59"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023848"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011658.2">
        <property type="protein sequence ID" value="ENSPNAP00000023848.1"/>
        <property type="gene ID" value="ENSPNAG00000000902.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434334"/>
    <dbReference type="CTD" id="2982"/>
    <dbReference type="GeneTree" id="ENSGT00940000158285"/>
    <dbReference type="OMA" id="NANFFGE"/>
    <dbReference type="OrthoDB" id="6127067at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008074">
        <property type="term" value="C:guanylate cyclase complex, soluble"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019934">
        <property type="term" value="P:cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090594">
        <property type="term" value="P:inflammatory response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070482">
        <property type="term" value="P:response to oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.260:FF:000002">
        <property type="entry name" value="guanylate cyclase soluble subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000007">
        <property type="entry name" value="Guanylate cyclase soluble subunit alpha-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1520.10">
        <property type="entry name" value="H-NOX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.260">
        <property type="entry name" value="Haem NO binding associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038158">
        <property type="entry name" value="H-NOX_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042463">
        <property type="entry name" value="HNOB_dom_associated_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655:SF4">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="14">
        <location>
            <begin position="487"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="681" mass="75756" checksum="B7C94024F20C67C8" modified="2018-12-05" version="1">MFCAKLKDLKISGECPFSVLVRNEEQGDFGGGRARDALPVSSEVQSSVESSVPKRKTSRSKVNLHSLGESVRKLVCPEFEKLRSAMVRVMKEEADLTCPAGDGTDLCEMLEKPEDLLDVMKIYSQKTGIHMDMLKISLGLEVFRDCYEEDSHILGVVGGALHDFLNSFNVLLKQSTPPSAEARQYVSQASVLCLDKDPGLLTVYYFNPHPTAELFFPGIIQAAARFLYCTQVEVRMDFAGAKDSGALQASHQPHLLYSVAVRDAKSLTPSPMRTHSSGDIPLSLLYTTFPFHIFLDQDMGLLQIGDGLRKRLGRSRDSQRRPTFEEHFAIVLPEIRANFQGILTMLNTQFILRVKQQQPSGSTASSGKHMDLKGQMIFMSEMNAILFLGSPCVDKLEELTGRGLYLSDIPIHNALRDVVLVGEQAKAQDGLKKRLGKAKAALEQAHQALEEEKRRTVELLFTIFPGNVAQRLWQGLPVQAKKFDRVTVLFSDIVGFTAICSRCTPMQVVNMLSELYTRFDGHCGELDVYKVETIGDAYCVAGGLHKESPTHAVQIALMAIKMMELSDEVTTPMGEVIRMRIGIHSGSVLAGVVGVKMPRYCLFGNNVTLANKFESCSLPRKINISPTTYRLLKDRPEFTFIPRSREELPPNFPSDIPGICYFLEASDRRTNDARRDSATET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKB1</accession>
    <name>A0A3B4DKB1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein shisa-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Scotin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the shisa family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560285.1">
        <property type="nucleotide sequence ID" value="XM_017704796.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKB1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023551"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012339.2">
        <property type="protein sequence ID" value="ENSPNAP00000023551.1"/>
        <property type="gene ID" value="ENSPNAG00000000671.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431556"/>
    <dbReference type="CTD" id="558619"/>
    <dbReference type="GeneTree" id="ENSGT00940000166256"/>
    <dbReference type="OrthoDB" id="9949323at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395:SF14">
        <property type="entry name" value="PROTEIN SHISA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395">
        <property type="entry name" value="SHISA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017289482" description="Protein shisa-5" evidence="15">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="16">
        <location>
            <begin position="22"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="167"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="258" mass="28097" checksum="726E1B8BB519ED32" modified="2018-12-05" version="1">MATTLPVLLLLSGLLFMVASDKDCDSYYTPSGGYRKSQDCGYLQYCCGSCSNRYCCSNPYMKLDDDLCLQDLHQFEQAKVLTSMKSSKSVNPITIGSAIAGVMTIVIFFLLCCLCPCCCLYKMCVKPRPVVTTTTHTTVLSTQYPQQQPLNPGAQYPAYQQIPAQPGYSAQPGYEGQPMPMPAAPYQGQPYAPGPPPPYHESGYPAPYSQAAYDGGQPPYPLQPPTQPGYAHPTPLTDYSATQPAYNPAYVDPPKTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKT9</accession>
    <name>A0A3B4DKT9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PPP6C</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Protein phosphatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed by the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). Interacts with subunits PPP6R1, PPP6R2 and PPP6R3. Interacts with subunit ANKRD28. Interacts with IGBP1. Interacts with MAP3K7. Interacts with NFKBIE. Interacts with TRIM14 and WRNIP1; these interactions positively regulate the RIG-I signaling pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKT9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011884.2">
        <property type="protein sequence ID" value="ENSPNAP00000023736.2"/>
        <property type="gene ID" value="ENSPNAG00000008266.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074961"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001878">
        <property type="term" value="P:response to yeast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000005">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="111"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023736.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="306" mass="35315" checksum="4E56D8E3B33B3760" modified="2024-11-27" version="2">KMAPLDLDKYVEIARQCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQVPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWRYCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPRGAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDERLVTVWSAPNYCYRCGNIASIMVFKDVNTREPKLFRAVPDSERVIPPRTTTPYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMK4</accession>
    <name>A0A3B4DMK4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tankyrase 1-binding protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017537662.1">
        <property type="nucleotide sequence ID" value="XM_017682173.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537664.1">
        <property type="nucleotide sequence ID" value="XM_017682175.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537665.1">
        <property type="nucleotide sequence ID" value="XM_017682176.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000009468.2">
        <property type="protein sequence ID" value="ENSPNAP00000024693.1"/>
        <property type="gene ID" value="ENSPNAG00000001311.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410877"/>
    <dbReference type="GeneTree" id="ENSGT00940000154184"/>
    <dbReference type="OMA" id="TMSPQDP"/>
    <dbReference type="OrthoDB" id="9950932at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032764">
        <property type="entry name" value="Tankyrase-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040006">
        <property type="entry name" value="TNKS1BP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22042:SF3">
        <property type="entry name" value="RIKEN CDNA 2900026A02 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22042">
        <property type="entry name" value="TANKYRASE 1 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15327">
        <property type="entry name" value="Tankyrase_bdg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01319">
        <property type="entry name" value="Tankyrase_bdg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tankyrase 1-binding protein C-terminal" evidence="2">
        <location>
            <begin position="1737"/>
            <end position="1914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="856"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1194"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1604"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="909"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1194"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1334"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1363"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1375"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1405"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1419"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1604"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1661"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1671"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1692"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1713"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1793"/>
            <end position="1810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1923" mass="218598" checksum="184A4DC4608F271A" modified="2018-12-05" version="1">MAAQVEIEAGRLLLSPLTDSSNKGLMEGSPGPQSSLIISPEPRKPVPGPKPRLTPKPFAVERNPTIRPIVAPKPHPKPRPEPTHLASPKPDKPEPPSAPKPQPASKPKPASGRPTSTSFKPGPTPGAGQASKPAAQTLKPSPTTEPSKPTPTQTGSVFRRTNLGPSTSLPKSSISPPRPAAEWSGASRPKPAGAPITRAKSMGFLPGAGTEGDGDKEADDVFTPQETGSRSSRPRPVSAIFLQDAPQSETKTTAAPTPAPRWMARRPLSADLTSKFESIGLSLHRQRPTKAESKENTPEPSAQDVTAPEDVGFKDKEPGESDGKVKASSSEKKEPEGEDTKLEEKGGSSIKRRISLLLDSSSSSFSVPVTKGVESRPPAQPIADTDGTVGVKQRIKELTEDLSTAPAPPQKPQFKPRPLPTDLTKRFASDAESPSSAEPRGLQEGAEDLSRKSEDPFSTGLSKTFDDRRLQDDLSVLSPSSSGMQTVRASMFDNVVERHSVQMVEDSSSSAGPVVKPMPRRNISLRAKADSVPKALKDEEDSSLVTATYREPESPSSPQRVEHVFDTLAAVEEIRAVSESVPAAHLEDRALTLRSRRSEGSEKPQQAIGGLAEDSLILGYNNQFKDKDVLPTDSTTEPCTTRVLRVGALHKWVESETKDVESDKQREMVRKMEEEIQRQMEVHMAARADLEEEEREAERQRHVEAERQRQVEAERQRQVEAERQRQMEAERQRQMEAERQRQMEAERQRQMEVERQMEVERQRQMEAERQRQMEAERQRQMEAERQRQMEAERQMEVERQRQMEAERQKQAERRREREEAAAAAKRPKMVETEEQPTPRPRATYFALTGQLNETVHQTAGVDQRGKTEEHVKTEVPFDDFSVKSGRWGYPSRSASIRRNPSMDPTLLKRSAEEWRRSEGRERTQRNSTPLKDKAIEEGLEMEKQKLAEYERIKDLERQKKQRELEIEKQKEIERERKKEMERQRELENEREREREMERQKEIEKQRELERRRELERQKQLEKERQKQIEYEKRKAAERELEQQREFERQRQKEYDREKERMLDQERLKMREFEKQKEIERQKQKEIERQKELEMQRELERQRELERQRELEKERQRQRELERQRELERQKELERQREQERQQELERQKQKELEHQREMQWWMQSERQKAEERERQREREELERLRELEKRQLLEFQQQKESERRAQQEREKDKHAARPNKERDEELQRVAEWNKQENEQERRTAGSPLRPKVLDLDSVSLGDRDGSPTGRWKQPSPHAEEAYRPSILDIDSFRNQTQPEASVDPFGMPEFALTKPVTPVHPQTLLQNLQPNTVPQHQQQGIPQPQYQLQQQFQSQPIQPQAPLQNLQPSTIPHYQQQGIPQPQYQLQPQFQSQPNPAPQPVYQLPQFQSQPQPSFHPSEQANQQTPLQPQTFGAERPKTQPQAFSQLDWASGVSQLGSGWHSSQAAPWGMSQGEVAVDEPPWITATEGSRRPISTRLSVEQMLQRQEDRVSGSVLSPTSAPSVTPIQTPAFASSVVSNQGPTMPPVSSIGTSPFSLLTPERVWTTPTDGASAPASLPRREAQTSLAVEPIWTPNWELASQELNQAQQRNKGTQDASRMRSRSVSRRSAPAESSVDGPLSRIRSRSAHRERVRPSWEQVKQSGHEEPKDTDTLVQETDSQYGTWDTGLRTDDSLTPATPSSDGNLTPSPRKPAPTHTPEEATPADTPTSPAPSAEEPPEPFSFPETPTALLDSSALRSRAQLSKRLSRRAPPSRNARHSAALAQLTEGSAAGGEEWRYRDSTEDKTDSKPQEDEESDSEEQVREPRMSSSSSASSQPQRVSLFPGMDPSALMAQLKKRGDGETQPDGPAPLSSQLSRSPKSPFLPRAARVLPPAGGKENGEASSPQWLKELKTKKRLSQYENDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DP59</accession>
    <name>A0A3B4DP59_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stathmin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the stathmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017549357.1">
        <property type="nucleotide sequence ID" value="XM_017693868.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DP59"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025258"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008064.2">
        <property type="protein sequence ID" value="ENSPNAP00000025258.1"/>
        <property type="gene ID" value="ENSPNAG00000010244.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425289"/>
    <dbReference type="CTD" id="100003563"/>
    <dbReference type="GeneTree" id="ENSGT01030000234597"/>
    <dbReference type="OMA" id="CNFMKSA"/>
    <dbReference type="OrthoDB" id="5986631at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000956">
        <property type="entry name" value="Stathmin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036002">
        <property type="entry name" value="Stathmin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10104">
        <property type="entry name" value="STATHMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10104:SF18">
        <property type="entry name" value="STATHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00836">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002285">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00345">
        <property type="entry name" value="STATHMIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101494">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51663">
        <property type="entry name" value="STATHMIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="179" mass="20719" checksum="CD9095DCBC50E141" modified="2018-12-05" version="1">MAKTAIAYKEKMKELSLVSLICSCFYPEAHNKMMAEFEDMKVKPINKRASGQAFEVILKPPSPTSDGYSITTPPKKRDVSLEDIQKKLEAAEDRRKSQEAQVLKALAEKREHERDVLLKAMEENSNFSKMAEEKLIMKMDHNKKNRKALLMAMLERLQEKERHAQGVRRNKELREELTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DRH4</accession>
    <name>A0A3B4DRH4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Membrane protein, palmitoylated 4a (MAGUK p55 subfamily member 4)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DRH4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025564"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007295.2">
        <property type="protein sequence ID" value="ENSPNAP00000025564.2"/>
        <property type="gene ID" value="ENSPNAG00000010716.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156444"/>
    <dbReference type="OMA" id="YECLLEY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06799">
        <property type="entry name" value="PDZ_MPP3-MPP4-MPP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="8">
        <location>
            <begin position="76"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="147"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="235"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="6">
        <location>
            <begin position="415"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025564.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="70720" checksum="5DE2373E2CBC8734" modified="2024-11-27" version="2">MRQHVETELMTHPGLHENSLTKILEDVVEEVRLSIDKDINGADLLHSLLSAPWLNSLLRVYECLLEYTRTSPRPCLLYASTLSQEIMRDMRSLTAPSPEAQELYMLLRARHFQALLSAHDSVAQKDYSPVLPPLPDDLPEDEEAMRIVCLVKNKQPLGATIKKDEKTGEIFIARVIHGGLADRSGLLYPGDKLVEVNGEPVEGLEPVQVIQILIHSQGTILFKVIPNSPQPPTSQATLYMRAMVDYNPLQDPSIPCPDAGMAFSKGDLLQIVDQRDVQWWQARLLPNTTDCAGLVPSPSHFKSKKREFWWSQSYQAHTCIKSLSAADVGEEEGAFEEKSADADDEAFETEEGEHSGHMEGFYIAGFRYSLRLWRRRAPRKRRQSCQSCNPNSCHSASTSPYEEVLNYQRNRSEPPRLIVLLGPSGVGVNELRRRLIKINPNTFQGPIPYTTRLPRSGEVNGREYHFITKELFEYLMVNHKFIEYGEHKRHLYGTCFDSIKDVLDSGKTCIIDIEPHCIHAVRTKKLKPYIIFVKPPSLEHMKLTRTDAKFIANSYFKTSFRDEDFEKIEEAARIMEAQYSQFFDCVIVNDRLQDTCMDLFAAIQHAQEGATVDTSQLDQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DS19</accession>
    <name>A0A3B4DS19_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphatidylinositol glycan anchor biosynthesis, class U</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PIGU</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563932.1">
        <property type="nucleotide sequence ID" value="XM_017708443.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DS19"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026263"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005545.2">
        <property type="protein sequence ID" value="ENSPNAP00000026263.1"/>
        <property type="gene ID" value="ENSPNAG00000011592.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433706"/>
    <dbReference type="CTD" id="128869"/>
    <dbReference type="GeneTree" id="ENSGT00390000014941"/>
    <dbReference type="OMA" id="ALWHLWI"/>
    <dbReference type="OrthoDB" id="549017at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
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        <location>
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            <end position="25"/>
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="435" mass="49943" checksum="B15696377B29AF72" modified="2018-12-05" version="1">MAAPLTLVLVLAVTIRAVLFRSSLAELISERVEVVSPLNAWKRVVEGLALLDLGVSPYSGDVFHETPLIIYLFHFVVDYAEIVFMIADGITAVCLYLAVQNYNKNVFRKQKYALEADRYPHDCLELMRSPREMHYIPLKVAMFYLLNPFTILSCVAKSTCGLNNAVIALFILSTLKGSALLSAIFLALATYQSIYPLTLFAPALLFLLQRLYIPVNLCRSSFWFFTLQYAFMFVGSLVVIVCLSFFLLGSWDFIPSVYGFILSVPDLTPNIGLFWYFFAEMFEHFRFFFICVFQINIFFYTIPLSIKLKEHPVFLIFMQIAIISIFKSYPTVGDIALYMAFLPAWSHLYRFLRNIFLVSCVLLACSALFPVLWHLWIYAGSANSNFYYAITLLFNVGQILLVSDYFYAYLRREHHLSQGLYLRRKDGSEATLVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0G9</accession>
    <name>A0A3B4E0G9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ITGB7</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575454.1">
        <property type="nucleotide sequence ID" value="XM_017719965.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000028759"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015215.2">
        <property type="protein sequence ID" value="ENSPNAP00000028759.1"/>
        <property type="gene ID" value="ENSPNAG00000014568.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440828"/>
    <dbReference type="CTD" id="3695"/>
    <dbReference type="GeneTree" id="ENSGT01150000286983"/>
    <dbReference type="OMA" id="CECSMDT"/>
    <dbReference type="OrthoDB" id="410592at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:leukocyte migration"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
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        <property type="entry name" value="EGF_integrin_2"/>
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        <property type="entry name" value="Integrin_bsu"/>
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    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
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        <property type="entry name" value="Integrin_bsu_VWA"/>
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        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF36">
        <property type="entry name" value="INTEGRIN BETA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrin_beta"/>
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    <dbReference type="PIRSF" id="PIRSF002512">
        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 18" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0401">Integrin</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017475398" description="Integrin beta" evidence="20">
        <location>
            <begin position="23"/>
            <end position="762"/>
        </location>
    </feature>
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        <location>
            <begin position="692"/>
            <end position="716"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="21">
        <location>
            <begin position="33"/>
            <end position="451"/>
        </location>
    </feature>
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            <begin position="714"/>
            <end position="761"/>
        </location>
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            <begin position="34"/>
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    </feature>
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            <begin position="617"/>
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            <dbReference type="ARBA" id="ARBA00007449"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR002512-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="762" mass="84657" checksum="1C4FE48D5B703B1F" modified="2018-12-05" version="1">MAVAYALVMVVLLQFVGQPCTAVQAPVCQPKASCSECLRSPGCAWCKQREFLKHGESTERRCDSFELLQSRKCNDVVNPAADKFISKDNKLNSKLEDVVQLKPQGINITLRIGMPEEFEVEFQRAEGYPIDMYYLMDLSYSMKDDLENIKNLGQDILKELQKVTQSVRIGFGSFVDKDKLPYVSQVKSRHQNPCPNRLDTCEPAFTFKNVLPLTEDARQFKKKVSAQKISGNLDSPEAGLDAIMQAAVCQKEIEWRDVTRILVYTSDDTFHIAGDGRLAGIFQPHDGRCHLSRDGFYDGTAFDYPSIGHLSRVLQANNIQLIFAVTKESVPAYEALSALIPQSVVGELKNDSSNVVRLIAEAYGNLSSTLLLEQDHTPAGLHISYMSYCSPEKPSEWQHRGECTGIKVNEKVAFKVRLNATECLKEPQKFEIRMQGIKEVLKVTVKTLCDCNCNDKQENSEVCKGQGTLSCGVCSCNEGHLGQYCECKRQSDMNSTHAILKNCRANEKSQVCSGHGSCECGECRCIRHFSGKFCECDDASCEHHNSKMCNGQGTCECGNCTCNENYSGPACQCSTDTAMCLNKNGQKCSGHGECECNQCKCRDGFGGKDCSQLLKPCPTYLPCVDCTVKAKLYSSPTNNCSVVCESVKLTRLEGKHDLQCLHDDVIYDVTQNDDGTVLIQYADLPRSIDKTMVAIVTSVTAIVLIGIVIIIVYRVLVELYDLREYRSFVKAQAQTEWEETNQNPLFQGATTTVLNPLHTQGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1C9</accession>
    <name>A0A3B4E1C9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger matrin-type protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ZMAT3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a bona fide target gene of p53/TP53. May play a role in the TP53-dependent growth regulatory pathway. May contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with dsRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017554951.1">
        <property type="nucleotide sequence ID" value="XM_017699462.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1C9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029530"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016728.2">
        <property type="protein sequence ID" value="ENSPNAP00000029530.1"/>
        <property type="gene ID" value="ENSPNAG00000015664.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428446"/>
    <dbReference type="CTD" id="64393"/>
    <dbReference type="GeneTree" id="ENSGT00730000111202"/>
    <dbReference type="OMA" id="YRGKNHA"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000285">
        <property type="entry name" value="Zinc finger matrin-type protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000612">
        <property type="entry name" value="Zinc finger matrin-type protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052644">
        <property type="entry name" value="ZMAT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46786">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46786:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="19">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="19">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="19">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="364" mass="40623" checksum="019AE36904070ECA" modified="2018-12-05" version="1">MAMNVRNRDAVYSSADYCNTYNLHPVTYGNSSHYFPRLPGPETVLKPPLNLMCQQHSQTFQPVSPTQTLGPPPVMMSPDPLAFPLCSPLPAKPPPHLQLVPSPPITIPQVSSPQLPEEECQKVGTEQDATLEELCKPLYCKLCNVTLNSAQQAQAHYQGKNHSKKLRNFYAGSQQPPPIRIPEEVEPISQQSLSTPPTEAATAVVKQPPFKGPSRVILATENDYCKLCDASFSSPAVAHAHYQGKNHAKRLRLAEAQQGTSSSDTVDLAQRRSRKEGNEYRLNKNRRTHVNTAMPGPYYNPRPRQRIPRDLAMCVTPSGQFYCSMCNSGASEEADFRLHLESKQHKSKVSEQRYRSEMENLGYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E238</accession>
    <name>A0A3B4E238_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Drebrin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Developmentally-regulated brain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DBN1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E238"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029758"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035736.2">
        <property type="protein sequence ID" value="ENSPNAP00000029758.1"/>
        <property type="gene ID" value="ENSPNAG00000016068.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159431"/>
    <dbReference type="OMA" id="EEHRWEA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045773">
        <property type="term" value="P:positive regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061003">
        <property type="term" value="P:positive regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098974">
        <property type="term" value="P:postsynaptic actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ADF_drebrin_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Drebrin 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Severin"/>
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        <property type="entry name" value="ADF-H"/>
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    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF1">
        <property type="entry name" value="DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="16">
        <location>
            <begin position="4"/>
            <end position="133"/>
        </location>
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        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
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            <begin position="279"/>
            <end position="430"/>
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            <begin position="474"/>
            <end position="503"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="317"/>
            <end position="334"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="475"/>
            <end position="485"/>
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            <dbReference type="ARBA" id="ARBA00004279"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
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    <evidence type="ECO:0000256" key="9">
        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="528" mass="59045" checksum="0BB7D074D3D932C5" modified="2018-12-05" version="1">MAVNLRTNRLTLLTAYQDVIDETSATDWALYTYEDNSNDLVLASSGGGGLEEITVTFDNSRIMYGFCSLKEATATLPRYILINWVGEDVPDARKCACASHVATIAEFFQGVDVIINASSMEDIEPSAIGQRLTNGTGPVASPVLSRLRTKDEENTEHVGTTYQKTNAEIEMKKINREEFWEQAKREEEMRKEDERKKAAEERQRFEEERMELERKEQEEREKRYRERERQIEEHRKKIQEEEEAKEKSRTQPLIVREAAAIIAQRTSNPREFFKQKEKAVASSVDTSPVATHRTDDSAEGIPDMAAVFPTPIPKIAIQEDKEDTSCKEWGKSPERAPVQESSPNKPVQSVAPQARSTDPSEDSLLDLWESGPPPATAPSQATHIMDLMGDAPEPLQAASSPNLPQPLLSFDETPDTTFCPGPEDDPSSLVDVAGPHQMTLSYQQALQHASGSDSQELDDSQLLMTNGDTLLKEGTQASEGYFSQSQEEDFAQSDECSAKVNPTPVFYNKPPEIDITCWDTDPVMDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E469</accession>
    <name>A0A3B4E469_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017570135.1">
        <property type="nucleotide sequence ID" value="XM_017714646.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570136.1">
        <property type="nucleotide sequence ID" value="XM_017714647.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E469"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030483"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018575.2">
        <property type="protein sequence ID" value="ENSPNAP00000030483.2"/>
        <property type="gene ID" value="ENSPNAG00000017240.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437509"/>
    <dbReference type="CTD" id="558213"/>
    <dbReference type="GeneTree" id="ENSGT00940000157143"/>
    <dbReference type="OMA" id="KHMAMPA"/>
    <dbReference type="OrthoDB" id="10067217at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035613">
        <property type="term" value="F:RNA stem-loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16781">
        <property type="entry name" value="mRING-HC-C3HC3D_Roquin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1790:FF:000001">
        <property type="entry name" value="roquin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000004">
        <property type="entry name" value="roquin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Roquin-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041523">
        <property type="entry name" value="ROQ_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048575">
        <property type="entry name" value="Roquin_1_2-like_ROQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052249">
        <property type="entry name" value="Roquin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139">
        <property type="entry name" value="RING FINGER AND CCCH-TYPE ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139:SF6">
        <property type="entry name" value="ROQUIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18386">
        <property type="entry name" value="ROQ_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21206">
        <property type="entry name" value="Roquin_1_2-like_ROQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="516"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="603"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="765"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="667"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="691"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="797"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030483.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1122" mass="122800" checksum="877F3649CDC2559D" modified="2024-11-27" version="2">MPVQAPQWTEFLLCPICTQTFEETVRRPISLGCGHTVCKMCLNKLHRKACPFDQTAINTDIEQLPVNSALLQLVGGQVPKLQPVALITSPEDTKHYDEARQCVEELALYLKPLSSARGVGLSNAAQSMLSRPMQRKLVTLVHCQLVEEEGRVRAMRAARSLGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDSQIVQIAMEGGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLEKGLVAVKTVVHGLVDFIQNHSKKGADQQQPPQHSKYKTYMCRDMKQKGGCPRGASCTFAHSQEELEKYRKMNKRLAPRLPCGPGLLPDEGVSLEGVTRKPSPLTNGIVAACPGTPVPPLLSRGPEPSPYELLRKPKVDAGSLSAPGSPPDTLDKAGLPLPSHTVAHGRGEHLPMPKQMPGMPRGAPPMYPQQQAELFYSEARAPSGSQYDAQYSAGYPYQHTQYVPPRYIRNPPPPGESAMAPYQDPYPGYGPERQYPGPHSGPPFSAVNPHPYPPPSHYDSRRHGPYAGPPPPPPSFAPPRDELVRMSPVPLDVPPAALPPPTSGVAGSLYHPESSSRERYPPEGYYPPVPHPGQMRSHVRDPYSRSQPSLDYLHRRRKELLNQLEERKVISPPPFAASPTLPSHAFPSDYPQEYIEDGSKAFGTREPDYAGQYSPWSCDTIGSYIGSKDPKPKDAMSAGAVEMMNMEGKSLREPPLDAQRRSAEAKDDDPIIPFGPLPTVSPFGAISRTSKTGYQTTGPVQAMASSQAAGSKHMTMAAEYSYGNPGGWSSVSYPQHQSMASPGHFSERLPVSAPDREQLKIELQQVNQQISQQTQIRGMEAASNSMLLQREASALAAQPPTTKWATGGAVSSEQLSLELHHVEREIGKRTREIAMENQVAHEVQFKLKATENGQPDHKAQLEELSLALGEVSNGASSIVPASSVGSSMLSLTSKTSSLSLCSGDQAASGAELQKNGVVHSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E5K4</accession>
    <name>A0A3B4E5K4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stimulator of chondrogenesis 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SCRG1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5K4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031050"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036505.2">
        <property type="protein sequence ID" value="ENSPNAP00000031050.1"/>
        <property type="gene ID" value="ENSPNAG00000018171.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009191"/>
    <dbReference type="OMA" id="QENTHIH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044306">
        <property type="term" value="C:neuron projection terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028063">
        <property type="entry name" value="SCRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17463:SF0">
        <property type="entry name" value="SCRAPIE-RESPONSIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17463">
        <property type="entry name" value="SCRAPIE-RESPONSIVE PROTEIN 1 SCRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15224">
        <property type="entry name" value="SCRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16556" checksum="655F8836967809FB" modified="2018-12-05" version="1">MKLNQKQLFKKVFKYTLNRAELRHSLTTQHSTQENTHIHTQDKMKVLLLVAILLLGLHAGTALPLNRWSCYRKVLKDRNCHNVVSGLEMMRPIDSLQNHYWEDKSCDMVCYCNFSELLCCPRDIFFGPKISFVIPCDTAEAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E623</accession>
    <name>A0A3B4E623_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome-releasing factor 2, mitochondrial</fullName>
            <shortName evidence="7">RRF2mt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Elongation factor G 2, mitochondrial</fullName>
            <shortName evidence="7">EF-G2mt</shortName>
            <shortName evidence="7">mEF-G 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">GFM2</name>
        <name evidence="7" type="synonym">EFG2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554202.1">
        <property type="nucleotide sequence ID" value="XM_017698713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E623"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031210"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019976.2">
        <property type="protein sequence ID" value="ENSPNAP00000031210.1"/>
        <property type="gene ID" value="ENSPNAG00000018467.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428029"/>
    <dbReference type="CTD" id="84340"/>
    <dbReference type="GeneTree" id="ENSGT00550000074890"/>
    <dbReference type="OMA" id="GPQFTFP"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04092">
        <property type="entry name" value="mtEFG2_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000053">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000033">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000008">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000005">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000514">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03059">
        <property type="entry name" value="mEF_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030851">
        <property type="entry name" value="EFG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="54"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="63"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="127"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="770" mass="84560" checksum="C4197585E43586CD" modified="2018-12-05" version="1">MQFGVPFSVLRGCVRHCRSCSSPACGWRAQWRRSYSFLKDDVKSLRAVVNPDISKIRNIGIMAHIDAGKTTTTERMLYYSGYTRALGDVDDGDTVTDYMAQERERGITIQSAAVTFDWKEYRINLIDTPGHVDFTLEVERALRVLDGAVAVFDASAGVEAQTLTVWRQADKHKIPCVCFLNKMDKPGASLSYSLESIKTKLKANPVLLQIPIGSGKGFIGLVDLITKQRMVWQGKSWADDGRLFESRVLEPTDDPELLEEVDQARGGLIEQVADLDDEFAELLLGKYSDNFDAVPAGKFQEAVRRVTLARKGVPVLCGSSLKNKGVQPLLDAITAYLPAPNERNHDLVRWYKDDLCALAFKVVHDKQRGPLVFLRIYSGTMKPQSAIYNINRNGTERMSRLLLPFADQHVEIPSLSAGNIALTVGLKQTVTGDTIVSSKASAAAAARRAQKGAEEKRGSALSNRESSSLVLAGVEVPDPVFFCSIEPPTMSKQADLEHALNCLQREDPSLKVRTDPDSGQTILCGMGELHIEILHDRIKREYNIETHLGPLQVAYRETILQSVTTTETLDRTLGEKRHIVNAELTVSPWSESATGTSYYIAYEEEVEAQISAEVREAVDSGVQSGYLQGPLLGYPVQGVSTVIRSVHVEPGTSLAMVSACISRCMLKALQQASGQVLEPVMALEVTVGEEYLSSVLGDLAQRRGCVRDIQSRHDNKVLLATVPLAEMMGYSTVLRTLSSGTATFSLELASYEPMNPQAQTSLLNKMAGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAP7</accession>
    <name>A0A3B4EAP7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein strawberry notch homolog 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SBNO2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a transcriptional coregulator, that can have both coactivator and corepressor functions. Inhibits the DCSTAMP-repressive activity of TAL1, hence enhancing the access of the transcription factor MITF to the DC-STAMP promoter in osteoclast. Plays a role in bone homeostasis; required as a positive regulator in TNFSF11//RANKL-mediated osteoclast fusion via a DCSTAMP-dependent pathway. May also be required in the regulation of osteoblast differentiation. Involved in the transcriptional corepression of NF-kappaB in macrophages. Plays a role as a regulator in the pro-inflammatory cascade.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with TAL1; this interaction inhibits TAL1 occupancy of the DCSTAMP promoter, leading to the activation of the DCSTAMP promoter by the transcription factor MITF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000022237.2">
        <property type="protein sequence ID" value="ENSPNAP00000032369.1"/>
        <property type="gene ID" value="ENSPNAG00000020260.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159946"/>
    <dbReference type="OrthoDB" id="421838at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071354">
        <property type="term" value="P:cellular response to interleukin-6"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002281">
        <property type="term" value="P:macrophage activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000342">
        <property type="entry name" value="Protein strawberry notch homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003990">
        <property type="entry name" value="Si:ch73-63e15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057332">
        <property type="entry name" value="SBNO_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF5">
        <property type="entry name" value="PROTEIN STRAWBERRY NOTCH HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25373">
        <property type="entry name" value="SBNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="261"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="611"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1383"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1190"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="620"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="660"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1383"/>
            <end position="1406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1440" mass="159864" checksum="C18ED94E6EAAA32B" modified="2018-12-05" version="1">MPSFSASSAMDGQNYPHPEGPQLDTGIFGLAPSTMESSVYSCTPWSFSQQPYNLFWPMQSGSQQLHSNGTSEMHMDVFSSCSLPDLDFLNSADFSQDLSCIDDPSNTSLFSSPADSLSEYADSLPTVPTIWDISTPTQNQELNSNRFQGLRSLEDINAIISTAPLGGIQRLHAQPEEEEEVEEEETEELGHADTYAEYKPSKSTIGRSHPDLVVETNTLSSVPPPDITYTLSIPVQIINGGLLSALQLEAIIYACQQHEVILQNNQRAGFLIGDGAGVGKGRTVAGIILENYLKGRKKALWFSVSNDLKYDAERDLQDIGAPNILVHALNKIKYGDTATSEGVLFATYSALIGESQAGGQLRTRLKQILDWCGSDFDGVIIFDECHKAKNATSTKMGKAVLDLQNKLPLARVVYASATGASEPKNMIYMSRLGIWGEGTPFRAFEDFLHAIEKRGVGAMEIVAMDMKVSGMYIARQLSFSGVSFRIEEIGLDDDFKVVYNKAAKLWAEALAVFTQAADLLGMMSRKSLWGQFWSSHQRFFKYLCIAAKVRRLVELAKKELEAGKCVVIGLQSTGEARTREVLDENDGHLDKFVSAAEGVFQSLVQKHFPLEKDRREKGAGSKRKRKPRGRHPKYPKQAADGRAVINISDDSSTESDPVDSDSNSSPDSVQDNSDDVIFVTHTNCHAARLEEMKQDLLNKISELGKELPLNTLDELIDKFGGPEKVSEMTGRKGRVVRRPDGSVRYESRAEQGHTIDHINIKEKDRFMNEDKLVAIISEAASSGISLQADRRVKNQRRRVHMTLELPWSADRAIQQFGRTHRSNQVTAPEYIFLISELAGERRFASIVAKRLESLGALTHGDRRATESRDLSKYNFENKYGTKALDKITKAILGHIESKVPPPKDYPGGDAMFFTEMKQGMIDVGIFCKESRFGISPEKDCSITKFLNRILGLEVHKQNSLFQYFTDNFDYLIEKDKKDGKYDMGILDLAPGNDEIYNETQETFLTPGNPQEGQVILHKISVDRGMPWEEALKKAETLSEPDEGFYLSHKLRGSHPCVLLAEQGRGHNLVVYKPNVGKQKQTENLEIIQKKYHKVTPEEAKDSWETQFKFSFRKCSHANWNGRCKKVDEGHECLQGMRLRQYHVLCGALLCVWKRVADVVADITNSSILQIVRLKTREHKQVGIKIPENCVTQVRDELDRLDKEVKRKRQEKELQAQEQRAAEQCRSLLQGGHRDYTEFFSQPPQLPSLSSLATHYTNPIQFTSLPTLPSFSSLPSLRNAIVGLPDLREHSPMPLSPDSTQAGLGAVEDFSLEAFAQGQQQQDVQQDIIDLLNSLSEISPCNSSSSDALPTATQQASMSLFSSSTIPSSSSSSSLFSPSLTPSSSSSSLSLFSSPSPSDQPSLALPSANCPSDTGINAKEVLNSMLRGSDSRQSVIHFISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EC68</accession>
    <name>A0A3B4EC68_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">THUMPD2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EC68"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034137"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025680.2">
        <property type="protein sequence ID" value="ENSPNAP00000034137.2"/>
        <property type="gene ID" value="ENSPNAG00000023224.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063557"/>
    <dbReference type="OMA" id="QWTSAVM"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF1">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="2">
        <location>
            <begin position="230"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034137.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="427" mass="47257" checksum="1DA5E9D480162D27" modified="2024-11-27" version="2">WFWPLARIVEHIPGRVFFCCRAALQELMRLKSAERLFLLLRKAPPTSLPKNPGITQVCSKVKCIFIRTFRLRFRLSFLYPINDAFLVLPPAKAASMIQQSIVGNPDVWRQALSTWAALQAELNGSHGRGQKRKREEEEDDVNGIKESVESEDACGLGQNRPTFRVSCRCSGAIARCCNSQNLSRIIGVAISQQLGWKADLRHCKVILCFAVYAVVNDLLSFISYCLFFDASVILDPMCGVGTILLEAAQECPSAVFIGMDSEEAQLQKAAENVEAAGQVNRMLLVQSSYIPLPTGSVDAVLCDVPFGRRFSCSTDMTSALPRILAEMERVLSDGGYLVLLLSLQLSGQIKKLVKSNTPNPESRAAGRNTKSPDHTHGVSKHSSEPDENRETERLKLSSLLLQNTHRVSLGSTDAIIHTYTKTHTLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECE4</accession>
    <name>A0A3B4ECE4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenomedullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECE4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033505"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024392.2">
        <property type="protein sequence ID" value="ENSPNAP00000033505.1"/>
        <property type="gene ID" value="ENSPNAG00000022122.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000288111"/>
    <dbReference type="OMA" id="IIPFHSE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010460">
        <property type="term" value="P:positive regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003073">
        <property type="term" value="P:regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051665">
        <property type="entry name" value="Adrenomedullin-reg_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414">
        <property type="entry name" value="ADRENOMEDULLIN, ADM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414:SF6">
        <property type="entry name" value="ADRENOMEDULLIN-5-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="152" mass="16663" checksum="61EB373A84FCD699" modified="2018-12-05" version="1">MVSSQSSSRSSPSSPANLGSRRRGHCMMTVAVLLFLYMPVTTAVAVRHSLRPQTDPLSLINSLQTAEEFVTSVDRHKGTPSTATNSRTTEVPTPLRNILIKSIRARSRRAVQRGCQFGTCQVHNLANTLYMMGQKTGKEQSKKATDPNGYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGM0</accession>
    <name>A0A3B4EGM0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phytanoyl-CoA dioxygenase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PHYHD1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">2-oxoglutarate(2OG)-dependent dioxygenase that catalyzes the conversion of 2-oxoglutarate to succinate and CO(2) in an iron-dependent manner. However, does not couple 2OG turnover to the hydroxylation of acyl-coenzyme A derivatives, implying that it is not directly involved in phytanoyl coenzyme-A metabolism. Does not show detectable activity towards fatty acid CoA thioesters.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PhyH family. PHYHD1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGM0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034361"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026121.2">
        <property type="protein sequence ID" value="ENSPNAP00000034361.2"/>
        <property type="gene ID" value="ENSPNAG00000023636.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006287"/>
    <dbReference type="OMA" id="KYSEDNW"/>
    <dbReference type="OrthoDB" id="445007at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883:SF15">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034361.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="293" mass="33373" checksum="74A7935BBCADD775" modified="2024-11-27" version="2">MDALTEQDVKKYREDGYLVLEGVFSPEECEELRARMRQIVERMDVPSHCRTQFTTDHDEQLKTQLQGNADYFITSGDKIRFFFEKGVFDDKGEFTVPREHSLNKVGHALHAHEPLFKSITHSPKIQTLGKKLGLISPVILQSMFIFKQPGIGGEVTPHQDATFLYTEPLGRVMGVWIALEDATLHNGCLWFIPGSHNNGITRRMVRTPKGSFPLTDFIGREQIYDDGLFVPAPVKKGGLVLIHGEVVHRSAENTSDASRHVYTFHIMESENTCWSPENWLQPTEELPFPSLYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGW5</accession>
    <name>A0A3B4EGW5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017574982.1">
        <property type="nucleotide sequence ID" value="XM_017719493.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017574983.1">
        <property type="nucleotide sequence ID" value="XM_017719494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGW5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038569.2">
        <property type="protein sequence ID" value="ENSPNAP00000034451.1"/>
        <property type="gene ID" value="ENSPNAG00000023764.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440568"/>
    <dbReference type="GeneTree" id="ENSGT00940000156888"/>
    <dbReference type="OMA" id="PLPICAC"/>
    <dbReference type="OrthoDB" id="1925236at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051868">
        <property type="entry name" value="ZN346_ZMAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144:SF3">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144">
        <property type="entry name" value="ZINC FINGER PROTEIN 385B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="227" mass="25129" checksum="507B7019570ACE40" modified="2018-12-05" version="1">MKSTAVDDSLFTASYCRVCSAQLISHSQRVAHYKSRKHANKVRLYYMLHPVDGGCPAKKLRTDNGSEDGDVDKNKCCTLCNMSFTSAVVAQSHYQGKIHAKRLKLLLGETTSIATTDSPGRPEQVVTDVSMVSPLTQTPLSLQFCLMCKACFNNPGMAQQHYSGKKHKKNTAQAFLLAQLGETLDTQHTNVLKQNRMCEVCSVTLNSVAQYHAHLQGSKHQNNLKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHC9</accession>
    <name>A0A3B4EHC9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SLC9A3R2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHC9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035897"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029552.2">
        <property type="protein sequence ID" value="ENSPNAP00000035897.2"/>
        <property type="gene ID" value="ENSPNAG00000026232.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182849"/>
    <dbReference type="OMA" id="CEVAHRT"/>
    <dbReference type="OrthoDB" id="10007415at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06768">
        <property type="entry name" value="PDZ_NHERF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015098">
        <property type="entry name" value="EBP50_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051067">
        <property type="entry name" value="NHER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14191:SF4">
        <property type="entry name" value="NA(+)_H(+) EXCHANGE REGULATORY COFACTOR NHE-RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14191">
        <property type="entry name" value="PDZ DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09007">
        <property type="entry name" value="EBP50_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="258"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="126"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="189"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="396"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="452"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035897.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="500" mass="54442" checksum="B895F3D9729CC6E9" modified="2024-11-27" version="2">MAYSVEREMLERGLQPRLCYLTKGDCGYGFHLHGERHCGAQFIRKIEPGSPADLAGLRSGDRVVEVNGLNVEKDSHHQVVEKIMAVEHRTRLLVVDRETDEFLHFHRLPCTEELAVEMGCLSPRSSSLASSPRTSCSSSPHVSHSSSPRDSITPPFSRGCSNKLRMAIAAKEMDKLAFIMNGNKTPPISPSVLLSSPSLHPQPITPPPHPPSPSTSLSETVCQTHLSATFTSPQEEAVAEEEAVMQDLRPRLCCMAMSEGGYGFNLHCAKNRMGQFVRSVDPDSPAERAGLRPGDRVVEVNDRSIEDMKHSEVVAFIRGGGGHAQLLVVDPETDALFKRLGITPTAEHLKEDCVDGPLIESPVSDRPNTDSSASPSPVTDGPLICPPIVNVTLTDPPITNGSLKHQSHGSSSSHSTLSETSAELSSSDAGNKMFDLSHCKCHSGLSGKQELKSQQESFLESDLRLSPTAAEAKQRARAKRANKRAPPMDWNKKKEIFSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EMS4</accession>
    <name>A0A3B4EMS4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; polyunsaturated fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017562208.2">
        <property type="nucleotide sequence ID" value="XM_017706719.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EMS4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037772"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041216.2">
        <property type="protein sequence ID" value="ENSPNAP00000037772.2"/>
        <property type="gene ID" value="ENSPNAG00000029800.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432696"/>
    <dbReference type="CTD" id="9415"/>
    <dbReference type="GeneTree" id="ENSGT00950000182990"/>
    <dbReference type="OMA" id="QWWKNKH"/>
    <dbReference type="UniPathway" id="UPA00658"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000010">
        <property type="entry name" value="Delta-6 fatty acyl desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF12">
        <property type="entry name" value="ACYL-COA 6-DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="16">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037772.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="493" mass="57660" checksum="03379AB41A327BB8" modified="2024-11-27" version="2">MSASSDKGGSALVRESSEDTHSSERLECRYSINQQKRHHCTGQRPLSAAGLMGGGTHQTSVEGRGASGVYTWEEVQKHSQRGDQWVVIERKVYNVSNWVKRHPGGHRVITHYAGEDASDAFMAFHPDQTFVRKFLKPLLVGELAPSEPSQDHKKNAALVNDFRALRDQFKAEGLFRTSPLFFCLHLGHILLLEALPLALLWYWGNGWINTILCIFILATAQSQAGWLQHDFGHLSVFKRSRWDHLLHKFVIGHLKGASAHWWNHRHFQHHAKPNVFKKDPDINMLNILVLGNVQPVEYGIKKLKHLPYNHQHQYFFLIGPPLLIPVYFNIHIFQTMIQQRDWVDFAWYLSYYIRYLSCFVPFYGVFGSFALLMCVRFIESHWFVWVTQMNHLSMDVDYEKHDDWLSMQLKATCNIEQSFFNDWFSGHLNFQIEHHLFPMMPRHNYIHAAPRVKELCKKYGIHYEVKGLWEAWCDIIRSLRKSGELWLDAYLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EMV1</accession>
    <name>A0A3B4EMV1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Troponin T2e, cardiac</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EMV1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033131.2">
        <property type="protein sequence ID" value="ENSPNAP00000037255.1"/>
        <property type="gene ID" value="ENSPNAG00000028755.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154709"/>
    <dbReference type="OMA" id="SRINEQQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030172">
        <property type="term" value="F:troponin C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031013">
        <property type="term" value="F:troponin I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000001">
        <property type="entry name" value="Troponin T, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF20">
        <property type="entry name" value="TROPONIN T2E, CARDIAC ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="93"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="29651" checksum="8E2A6FD6A7C06922" modified="2018-12-05" version="1">MSDTEEVVEEEVTEEAPAEEETTDEMKPKPKMFMPNIAAPKIPEGEKVDFDDIHRKRQEKDLSELQSLIEAHFIQRKKDEEELIALVNRIEKRRAERAEQQRIRTEKEKERQARLAEEKERREQEEQRKKQDEDAKKKKALTNMTHQYGGIQQKMEGRKGAKKQTEREKKRKILAERRKPLNIDHLSEDKLKEKANELWQWMMELEAEKFDLSEKLKRQKYDITQLLARVRDHQSAKGRGKGKVGGRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJP6</accession>
    <name>A0AAR2IJP6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Charged multivesicular body protein 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557154.1">
        <property type="nucleotide sequence ID" value="XM_017701665.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJP6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065076.1">
        <property type="protein sequence ID" value="ENSPNAP00000038209.1"/>
        <property type="gene ID" value="ENSPNAG00000035920.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429718"/>
    <dbReference type="CTD" id="445397"/>
    <dbReference type="GeneTree" id="ENSGT00720000108863"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="24235" checksum="2A3EB63492DC19DA" modified="2024-11-27" version="1">MGNIFGKKKRSRVTEQDRAVLQLKQQRDKLKQYQKKVTLQMEKERQLAKQLLKDGKKEKALLLLKKKRFQEQLLDKTENQISNLERMVQDIEFAQIEMKVIEGLKVGNDCLKKMHEVLSIEEVERIMDETHDSIEYQKQIDDILAGSLTQEDEDAVLAELEAITQGDVDVELPEVPDEQLPEVPEQEEPEREKEKVKKKPEREMLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IQA5</accession>
    <name>A0AAR2IQA5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IQA5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082636.1">
        <property type="protein sequence ID" value="ENSPNAP00000040214.1"/>
        <property type="gene ID" value="ENSPNAG00000034499.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165985"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021536">
        <property type="term" value="P:diencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000031">
        <property type="entry name" value="Homeobox protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016233">
        <property type="entry name" value="Homeobox_Pitx/unc30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882:SF1">
        <property type="entry name" value="PITUITARY HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000563">
        <property type="entry name" value="Homeobox_protein_Pitx/Unc30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="52"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="15">
        <location>
            <begin position="247"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="54"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="20"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="273" mass="30467" checksum="DD9490C9CE21DF87" modified="2024-11-27" version="1">SELIQLTDLLAREAVLSRSDHSAEYRSQIRGGAPDGKQKERACGVPEDQKQKKARRQRTHFTSAQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQMDLCKSGYLPQFSGLVQPYEDMYPPYTYNNWTNKGLGPTPLSTKSFPFFNSMSPLTSQSVFSAPSSISSMTMASGMAHSAVSAVPTAGINNISNLNGIGSSSIGSSMSSAPCHYGPPTTPYSVYRDSCSSSIAPLRLKPKQHPPFSYSPGSCQYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ISI4</accession>
    <name>A0AAR2ISI4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ISI4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070412.1">
        <property type="protein sequence ID" value="ENSPNAP00000040999.1"/>
        <property type="gene ID" value="ENSPNAG00000037524.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271821"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015095">
        <property type="entry name" value="AlkB_hom8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09004">
        <property type="entry name" value="ALKBH8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="399" mass="44944" checksum="394AC99174B2BA8A" modified="2024-11-27" version="1">MKTATIIPVPKSSAVTGLNDYRPVALTPIVTKCFERLVQTHIKATINVTVDPHQYAYRRNRSTEDAISSVVHTALTHLEQKDSYVRMLFVDFTSAFNTMTPQTLITKLSSLGLSSSMCNWVLDFLTNRPQSVRIHNISSPTITLSTGFPQGCVLSPLLFTLLTYDCSPIHPGCHIVKFADDTAVVGCITNRDESNYRQEVEHLEGWCRENNLCINVKKTKEMIVDFRRGHHDHLPLHIGGSEVEVVDSYRYLGVHLSSNLTWSNNTSTLVRKAHQRLYFLRRLRRAGLGRAVLTSFYRCVVCSSINVWHGSCSAADRKALQRVVKAAQRIVGVSLPSTTDIYTSRCRKRATCIRKDPTHPAHALFVPLPSGRRLRSIKCKTTRLRNSFIPEAVRLLNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITZ2</accession>
    <name>A0AAR2ITZ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ITZ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074214.1">
        <property type="protein sequence ID" value="ENSPNAP00000041071.1"/>
        <property type="gene ID" value="ENSPNAG00000030236.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271821"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015095">
        <property type="entry name" value="AlkB_hom8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09004">
        <property type="entry name" value="ALKBH8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="376" mass="42479" checksum="A11BC639B2520F6D" modified="2024-11-27" version="1">MDIILCFERLVQTHIKATINVTVDPHQYAYRRNRSTEDAISSVVHTALTHLEQKDSYVRMLFVDFTSAFNTMIPQTLITKLSSLGLSSSMCNWVLDFLTNRPQSVRIHNISSPTITLSTGSPQGCVLSPLLFTLLTYDCSPILPGCHIVKFADDTAVVGCITNRDESNYRQEVEHLEGWCRENNLCINVKKTKEMIVDFRRGHHDHLPLHIGGSEVEVVDSYRYLGVHLSSNLTWSNNTSTLVRKAHQRLYFLRRLRRAGLGRAVLTSFYRCVVESVVCSSINVWHGSCSAADRKALQRVVKAAQRIAGVSLPSTTDIYTSRCRKRATCIRKDPTHPAHALFVPLPSGRRLRSIKCKTTRLRNSFIPEAVRLLNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IXW2</accession>
    <name>A0AAR2IXW2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="17">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ATP8B4</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017563790.1">
        <property type="nucleotide sequence ID" value="XM_017708301.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017563791.1">
        <property type="nucleotide sequence ID" value="XM_017708302.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IXW2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057470.1">
        <property type="protein sequence ID" value="ENSPNAP00000044515.1"/>
        <property type="gene ID" value="ENSPNAG00000014682.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433619"/>
    <dbReference type="CTD" id="79895"/>
    <dbReference type="GeneTree" id="ENSGT00940000160101"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140327">
        <property type="term" value="F:flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000025">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000188">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF80">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE IM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 16 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="334"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="888"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="921"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="971"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1010"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1040"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1084"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="31"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="857"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1165"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1200"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1168"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1209"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="14">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="553"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
            <position position="576"/>
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        <ligand>
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        </ligand>
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        <location>
            <position position="610"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
            <position position="690"/>
        </location>
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        </ligand>
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        </ligand>
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        <location>
            <position position="692"/>
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        <location>
            <position position="805"/>
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            <dbReference type="RuleBase" id="RU362033"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYT4</accession>
    <name>A0AAR2IYT4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibroblast growth factor</fullName>
            <shortName evidence="5">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540543.1">
        <property type="nucleotide sequence ID" value="XM_017685054.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYT4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080442.1">
        <property type="protein sequence ID" value="ENSPNAP00000042756.1"/>
        <property type="gene ID" value="ENSPNAG00000033353.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108412830"/>
    <dbReference type="CTD" id="559630"/>
    <dbReference type="GeneTree" id="ENSGT00940000158380"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000004">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="200" mass="22422" checksum="85DCE0DC553833BB" modified="2024-11-27" version="1">MGAVGFPQVHGVQGLAPSGAHFALAQHQQQQLVSAERLSRSAAPDLALLKGALRRRQLYCRTGHHLQILPDGTVQGTRQDHSRFGILEFISLAVGLVSIRGVDSGLYLGMDSKGQLYGSEKLTAECVFREQFEENWYNTYASNLYKHGERGPHYFVALNKDGTSRDGTRSRRHQRFTHFLPRPVDPERVPELYKEVLGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZU5</accession>
    <name>A0AAR2IZU5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZU5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058283.1">
        <property type="protein sequence ID" value="ENSPNAP00000043111.1"/>
        <property type="gene ID" value="ENSPNAG00000035745.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000081666.1">
        <property type="protein sequence ID" value="ENSPNAP00000079339.1"/>
        <property type="gene ID" value="ENSPNAG00000034872.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000086104.1">
        <property type="protein sequence ID" value="ENSPNAP00000045435.1"/>
        <property type="gene ID" value="ENSPNAG00000035848.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000274254"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF73">
        <property type="entry name" value="INTERFERON BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044712491" evidence="8">
        <location>
            <begin position="20"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="146" mass="16713" checksum="AD97E28B425CA30C" modified="2024-11-27" version="1">MGALKVLSIVFVVLHVADAAVIPCTWTQFRLRDLNEESIMLLEKMGASLPLRCLEEGWRALPDDVFLNAQNEDLALVALETLRGVDRIFQNEQTSVTWDRETLGLFKNIISSRQVENLQKCGFSDCAWEMVRNEKNLHTQKIDSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J5U4</accession>
    <name>A0AAR2J5U4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Thioredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TXNL4B</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Essential role in pre-mRNA splicing. Required in cell cycle progression for S/G(2) transition.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Interacts with the U5-102 kDa protein subunit of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DIM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557379.1">
        <property type="nucleotide sequence ID" value="XM_017701890.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J5U4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067082.1">
        <property type="protein sequence ID" value="ENSPNAP00000045614.1"/>
        <property type="gene ID" value="ENSPNAG00000031179.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429844"/>
    <dbReference type="CTD" id="54957"/>
    <dbReference type="GeneTree" id="ENSGT00390000010779"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02986">
        <property type="entry name" value="DLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000059">
        <property type="entry name" value="Thioredoxin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF4">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017199">
        <property type="entry name" value="mRNA_splic_U5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="149" mass="17206" checksum="C0EF4984337AFEF4" modified="2024-11-27" version="1">MSFFLPKLSCKKEVDDAIKTVAEKVVVLRFGRDEDPVCLQLDEILSKTSHGLKNMTSIYLVDVDKVPVYTRYFDISYIPSTVFFFNGQHMKVDYGSPDHTKFVGCFKTKQDFIDLVEVIYRGAMRGKLIVQSPIDPRNIPKYDLLYHDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J7D2</accession>
    <name>A0AAR2J7D2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RETREG1-3/ARL6IP-like N-terminal reticulon-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RETREG1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RETREG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J7D2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079531.1">
        <property type="protein sequence ID" value="ENSPNAP00000046232.1"/>
        <property type="gene ID" value="ENSPNAG00000033709.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166973"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057282">
        <property type="entry name" value="RETREG1-3-like_RHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043384">
        <property type="entry name" value="RETREG1/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28659">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28659:SF3">
        <property type="entry name" value="RETICULOPHAGY REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24456">
        <property type="entry name" value="RHD_RETREG1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="RETREG1-3/ARL6IP-like N-terminal reticulon-homology" evidence="11">
        <location>
            <begin position="12"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="335" mass="36793" checksum="23B38D5A7C403D71" modified="2024-11-27" version="1">MVKAFEMASEDHGSGLSWEMVDSGQEGRSGAEAQFTDSWMSFKLFLAETSSFKQQNPGKFCLLVCSLCSFLAVLGRYIPGIVISYIIVLGIFLWPLVSSHEFGMWLDPVLQKLNFGVREFLQKIKENHEQRLLKAQTEKESIEADLSSLFPKLDSTACRELSVSDTEVSEITWTDNGTFNLSEGHTPQTENSEDLDRRSEEEVFSGGLPDFPSLDNGLGTNGDDDDLSIGLPTQGERVEGEAAAQALELVQRMAGDVITAAVTTAMQERLQAVGVPALAQALAEDTDSEAEDFELLDQSELEQLEGELGLEKPSDETQPKGTKQPGFFSKLLGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J893</accession>
    <name>A0AAR2J893_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">MMP24</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="19">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J893"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068294.1">
        <property type="protein sequence ID" value="ENSPNAP00000046459.1"/>
        <property type="gene ID" value="ENSPNAG00000001728.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158315"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021805">
        <property type="entry name" value="Pept_M10A_metallopeptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF138">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="24">
        <location>
            <begin position="113"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="332"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="381"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="428"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="477"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="288"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="active site" evidence="17">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="19">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
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    <feature type="binding site" evidence="19">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="183"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="185"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="190"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="191"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="198"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="207"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
    </feature>
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        <ligand>
            <name>Ca(2+)</name>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
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            <position position="213"/>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
    </feature>
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            <position position="216"/>
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        <ligand>
            <name>Ca(2+)</name>
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        <ligand>
            <name>Ca(2+)</name>
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            <position position="236"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
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            <position position="240"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <position position="246"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <location>
            <position position="254"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
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        <location>
            <position position="385"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="387"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
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        <location>
            <position position="434"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="20">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="600" mass="68939" checksum="8F70E4AE967B1217" modified="2024-11-27" version="1">RNHALLSHRHTLGSCCQTLLSHGYDLIISFPSSCDHTWLKNYGYLLPDDIRTSDLQSEKAMQSAVAAMQRFYGIPVTGVLDQTTIEWMKKPRCGVPDHPHISRRRRNKRYALTGQKWRDKKISYSIHNFTPKVGEKDTQRAIRQAFDVWQTVTPLTFQEVAYSEIKKEGKEADIMIFFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNANHDGNDLFLVAVHELGHALGLEHSNDPSAIMAPFYQYMETHNFKLPLDDLQGIQKIYGVPTAMLEPTRPLPTLPARRTHSTSERKHDRQSRPPRPPSGDRPSSPGYGKPNICDGNFNTVAFFRREMFVFKDRWFWRLRNNKVQEGYPMLIDQFWKGLPPRIDAAYERSDGKFVFFKGDKYWVFKEVTAEPGYPHSLVELGNCLPRDGIDTALRWEPVGKTYFFKGDQYWRYNEEKRTADPGYPKPITVWKGIPEAPQGAFVSREGFYTYFYKGKEYWKFDNQKLTVEPGYPKSILKDWMGCDQSEMEKNKDRHLPPDDVDIMVAINDVPSTVNAIAVVIPCILSLCILVLVYTIFQFKNKAVPQHVTYYKHPVQEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDM2</accession>
    <name>A0AAR2JDM2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Integrin beta-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ITGBL1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JDM2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055948.1">
        <property type="protein sequence ID" value="ENSPNAP00000047946.1"/>
        <property type="gene ID" value="ENSPNAG00000012428.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286919"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000036">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000249">
        <property type="entry name" value="Integrin subunit beta like 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000481">
        <property type="entry name" value="Integrin subunit beta like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000042">
        <property type="entry name" value="Integrin subunit beta-like 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000175">
        <property type="entry name" value="Integrin subunit beta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF3">
        <property type="entry name" value="INTEGRIN BETA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0401">Integrin</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="138"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="271"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="450"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="489" mass="53092" checksum="FF86D8DFBA8D7158" modified="2024-11-27" version="1">MRKLPLKLENPLKGIPPIFQTFCIIQGECCVSCRQVGGVCKLSTESSQRRCQTSDGLICSGRGECECGVCICQVTDPGKYYGPRCECHDWVCATYDGKTCNGHGTCECGKCICDEGWFGEACQHQKNCKLSSRKSKDLCRNAQGVVCSNAGTCHCGSCKCHNPDGKGLISGRHCECDDSQCLDEDSGEVCGGHGKCYCGNCYCSAGWHGDKCEFQCDISPWESKRRCTSPDGKICSNRGTCVCGECTCYDVDPSGDWGDIHGDTCECDERSCHATYDRYSDDFCSGHGQCNCGRCDCKEGWTGKKCEHPLSCSLSAEASLKKCRGSSNLPCFGRGLCLCGQCTCHPPGDSRVHGKNCECDDRQCEDVNGDICGGHGFCSCGRCICSDGWFGKHCQFPRSCNMTDTQSKSLCETAHGVMCSGKGSCHCGRCICSPQEWYISGDFCECDDRECDQHDGLICTGNGMCNCGTCECWDGWTGNACEIWVGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JG46</accession>
    <name>A0AAR2JG46_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JG46"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061749.1">
        <property type="protein sequence ID" value="ENSPNAP00000051008.1"/>
        <property type="gene ID" value="ENSPNAG00000036869.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="30" mass="3134" checksum="6B10D4BEE58B76A3" modified="2024-11-27" version="1">MTGCNKILTAIFQHLVVEAVIAAKGGPTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JK29</accession>
    <name>A0AAR2JK29_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JK29"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053742.1">
        <property type="protein sequence ID" value="ENSPNAP00000050201.1"/>
        <property type="gene ID" value="ENSPNAG00000030154.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="24"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="141" mass="15646" checksum="234CBDC9C1EBFA88" modified="2024-11-27" version="1">MITHQKAIMTLISIFIWMIITCTPVSLGRVVLTQSGDQTVQAGKTVSITCKHDPAIDCYDTATKKKDCVSWYHQLPGETPKLLIIWATESYSGVPSRISGSRAGNKIDYTFTITGVQPEDSGVYYCQSQHFLNSTFSVFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JT26</accession>
    <name>A0AAR2JT26_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ETS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JT26"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078505.1">
        <property type="protein sequence ID" value="ENSPNAP00000053344.1"/>
        <property type="gene ID" value="ENSPNAG00000030438.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167160"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000014">
        <property type="entry name" value="Protein c-ets-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF289">
        <property type="entry name" value="ETS-LIKE PROTEIN POINTED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNT" evidence="5">
        <location>
            <begin position="48"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="4">
        <location>
            <begin position="305"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="43290" checksum="747193D8FFEE580A" modified="2024-11-27" version="1">MISLYFLISVSTEFIGKQNHIFFPESDSLEVPPLTPTSKELLGQALNATFAGFTQERNRLHFPQDPRLWSEWEVSYWLDWCQAEFGLKNLNSELRNMPGSKLCSLDRETFLEWVADCTAGEILWEHLETMHKGKELLQSVRMCHINAVCFYLPSNAWFVSSLCVLESEYDHPAPLVLCASSSLCQSQLNIEKTLDCEELRVTTSTAWVGPPPLKFQQGEESVCKELLLHSAHPHITFKEFVREKVNLSSAVVPAAVLAGYTGMTLKQLVTVLRYFFAFLLCRLSRMVLSHSAKICILIHFHVVSQVAQLWGQRKNKPKMNYEKLSRGLRYYYDKNIIHKSAGKRYVYRFVCNLKGLLGYEPGELHAMLDIPASVNEGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JY48</accession>
    <name>A0AAR2JY48_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017538198.1">
        <property type="nucleotide sequence ID" value="XM_017682709.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JY48"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085649.1">
        <property type="protein sequence ID" value="ENSPNAP00000056925.1"/>
        <property type="gene ID" value="ENSPNAG00000032981.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411239"/>
    <dbReference type="GeneTree" id="ENSGT00390000009313"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00220">
        <property type="entry name" value="VMO-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.20">
        <property type="entry name" value="Vitelline membrane outer layer protein I (VOMI)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005515">
        <property type="entry name" value="VOMI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036706">
        <property type="entry name" value="VOMI_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18841:SF0">
        <property type="entry name" value="VITELLINE MEMBRANE OUTER LAYER 1 HOMOLOG A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18841">
        <property type="entry name" value="VITELLINE MEMBRANE OUTER LAYER PROTEIN I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03762">
        <property type="entry name" value="VOMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51092">
        <property type="entry name" value="Vitelline membrane outer protein-I (VMO-I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043624873" evidence="1">
        <location>
            <begin position="20"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="210" mass="23124" checksum="6D4B8918071586AE" modified="2024-11-27" version="1">MHHLVCITVILFLASFGSSVKGNRAERSIDRPFKSMLIVDNGHRWGTWGQKEMCPSGFYAAGFSLKVAGHWESMLVGDNTALNGIRLHCVDISKGKSGPYTDYATVQSDTGSWGKWKDIKWCKSGTLVSFQLRVEAYQGAFDDTAANNIKFKCSKDDEELVGDGTSWGDWGSWSDKCDGKGICGIETMVEQPRGLGDDTALNDVRLFCCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K3F4</accession>
    <name>A0AAR2K3F4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CAPZA1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions. Forms, with CAPZB, the barbed end of the fast growing ends of actin filaments in the dynactin complex and stabilizes dynactin structure. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K3F4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084774.1">
        <property type="protein sequence ID" value="ENSPNAP00000056646.1"/>
        <property type="gene ID" value="ENSPNAG00000002488.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183119"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030863">
        <property type="term" value="C:cortical cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008290">
        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.60:FF:000001">
        <property type="entry name" value="F-actin-capping protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.210:FF:000002">
        <property type="entry name" value="F-actin-capping protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.60">
        <property type="entry name" value="F-actin capping protein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.210">
        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042276">
        <property type="entry name" value="CapZ_alpha/beta_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042489">
        <property type="entry name" value="CapZ_alpha_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017865">
        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF5">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01267">
        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00748">
        <property type="entry name" value="F_ACTIN_CAPPING_A_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00749">
        <property type="entry name" value="F_ACTIN_CAPPING_A_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0117">Actin capping</keyword>
    <keyword evidence="5 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="295" mass="33925" checksum="F3A133D0BD6A6779" modified="2024-11-27" version="1">QCRFKKQLEGEPNTFSYLLFVQVRIAANFVTHAPPGEFNEVFNDVRLLLNNDNLLREGASHAFAQYNMDQFTPVKLENSEESVLITEHGDLGQGRYFDPRSRKSFRFDHLRKEASDPQPYQGETALHSWRDACDTALSAYVKEHYPTGVCTVYGKTVDGQKTIIACIEGHQFQPKNFWNGRCRSEWKFTISQSSSQVVGVLKIQVHYYEDGNVQLVSHKEVEDSIIVSDEESTAKEFVKIIENAENDYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K7X3</accession>
    <name>A0AAR2K7X3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ITLN1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K7X3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064546.1">
        <property type="protein sequence ID" value="ENSPNAP00000058589.1"/>
        <property type="gene ID" value="ENSPNAG00000030969.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154757"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000015">
        <property type="entry name" value="Intelectin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16146">
        <property type="entry name" value="INTELECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16146:SF46">
        <property type="entry name" value="INTELECTIN-1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043322167" description="Fibrinogen C-terminal domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="6">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="303" mass="33605" checksum="195815ED7F39A0C8" modified="2024-11-27" version="1">MMNWILLLVMTSFIEVSESERVMEYFSPRSCKELMEKYKTKEDGLYYLTTASGVVYETYCDMTTAGGGWTLVASVHENNMYGKCTVGDRWSSQQGSDPNVPDGDGNWANRVTFGTAEAATSDDYKNPGYYDITAQDVSVWHVPNNEQLDRWTQSSFLRYHTETNFLNDYGGNLFQLFTRYPVRYNAGSCPSNNGPSIPVVYDVGDATSNVNLYGPHIRGTAEAGFITFRVLNNEKAAMAICSGIKPNTCHSEHACVGGGGHFPEAAPRQCGDFASMDWDGYGTHAGWSASKQLTEAAILIFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KD11</accession>
    <name>A0AAR2KD11_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PIGBOS1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KD11"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072590.1">
        <property type="protein sequence ID" value="ENSPNAP00000060332.1"/>
        <property type="gene ID" value="ENSPNAG00000030159.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000178971"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="entry name" value="PIGBOS1"/>
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    <dbReference type="Pfam" id="PF23670">
        <property type="entry name" value="PIGBOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8546" checksum="11A67AEA22963467" modified="2024-11-27" version="1">MLPRRIPFNQIAFATLVGVGGGIYIYRPMFEPPRKPTKLSHKMALLCWQKKKEKKTELMSVLSFSYFDISLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KFB4</accession>
    <name>A0AAR2KFB4_PYGNA</name>
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        <recommendedName>
            <fullName evidence="4">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KFB4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050837.1">
        <property type="protein sequence ID" value="ENSPNAP00000060796.1"/>
        <property type="gene ID" value="ENSPNAG00000033003.1"/>
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        <property type="entry name" value="PROTEIN (PUTATIVE)-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21698:SF4">
        <property type="entry name" value="PROTEIN (PUTATIVE)-RELATED"/>
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    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="369"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="45186" checksum="537F50AB27E17A67" modified="2024-11-27" version="1">MSVYLSIYLSVCLSVCLSIYLSICLSVCLSIYLSIYLYVCLSIYLSVYLSVCLSVCLSVYLSICLSVCLSVCLSIYLSIYLSIYLSIYLSICLSIYLSICLSIYLSICLSVCLSVCLSIYLSICLSVCLSIYLSIYMSVYLSIYLSVCLSVCLSVCLSICLSVCLSVCLSIYLSIYMSVYLSVCLSVCLSIYLSVCLSVCLSVYLSIYLSIYLSIYLSICLSICLSVCLSVCLSIYLSIYLSVCLSVYLSIYLYVCLSIYLSIYLSICLSVCLSVCLSVCLPIYLSVCLSVCLSVYLSIYLSIYMSVYLSIYMSVYLSIYLSIYLSIYLSIYLSVCLSVCLSVCLSVYLSVCLSVYLSICLSIYLSIYLSIYLSIYLSVCLSVCLSIYLSIYLYVCLSIYLSIYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHM5</accession>
    <name>A0AAR2KHM5_PYGNA</name>
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        <recommendedName>
            <fullName evidence="7">Polyadenylate-binding protein</fullName>
            <shortName evidence="7">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PABPC4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHM5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046603.1">
        <property type="protein sequence ID" value="ENSPNAP00000061959.1"/>
        <property type="gene ID" value="ENSPNAG00000024355.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154788"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000001">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000021">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000042">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000154">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="12"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="100"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="192"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="295"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="10">
        <location>
            <begin position="560"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="646" mass="72049" checksum="99B9F601BE0C24B7" modified="2024-11-27" version="1">MNAATAGSYPMASLYVGDLHPDITEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPPDAERALDTMNFDVVKGKPIRIMWSQRDPSLRKSGVGNVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYAFVHFETQDAADRAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEDMDDDRLKDIFDKYGKTLSVKVMTDPTGKSRGFGFVSYEKHEDANKAVEEMNGTELNGKTVFVGRAQKKMERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSITSAKVMLEEGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEERKAHLTNQYMQRIAGMRAMPANAIINQFQPTSGYFMPAVPQAQNRTTYYAPNQLAQMRPNPRWQQQGGRGQGGFQGMPNSLRQPGPRANIRHLGPSGTAQGPRGIPTGAQRVGEQSPLTGTCLKYADITDVNESLSGTGQPMGPRPTLGVTTPRAMPPYKYATTVRNTQPQVVQPIAQPAVHVQGQEPLTASMLAAAPPQEQKQMLGERLFPLIQAMHPSLAGKITGMLLEIDNSELLHMLESHESLRSKVRVRQAHFHSLGLLKDELADKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJJ6</accession>
    <name>A0AAR2KJJ6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Si:ch211-63b16.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KJJ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083980.1">
        <property type="protein sequence ID" value="ENSPNAP00000062291.1"/>
        <property type="gene ID" value="ENSPNAG00000031641.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000278259"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="6"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="103" mass="11127" checksum="90A8052214F488AD" modified="2024-11-27" version="1">CLFTAEPQVSVRVSGLNSVTAETGRGHRAHVLDFDAVWSEGASLRDVYESTAKPLVEWVIRGYNGCLILYGPSNSVDPHSVQSSSCRLKGIISQAADQIFSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KK06</accession>
    <name>A0AAR2KK06_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="RefSeq" id="XP_037401667.1">
        <property type="nucleotide sequence ID" value="XM_037545770.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000082562.1">
        <property type="protein sequence ID" value="ENSPNAP00000062799.1"/>
        <property type="gene ID" value="ENSPNAG00000035567.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108436580"/>
    <dbReference type="GeneTree" id="ENSGT00940000166813"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12881">
        <property type="entry name" value="SPRY_HERC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14401">
        <property type="entry name" value="UBA_HERC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000015">
        <property type="entry name" value="LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase HERC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000006">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035768">
        <property type="entry name" value="SPRY_HERC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF6">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="379"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="429"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="484"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="537"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="588"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="640"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="13">
        <location>
            <begin position="691"/>
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</entry>
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                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="40368" checksum="19F852F4F4E70407" modified="2024-11-27" version="1">MDKFRMMFQFLQANQESFMNGICGIMALASVQLYSSFEFTCPCLPEYNLAYGLGILLVPAVWFFMLGFVLNNNISMVTEEWRRPAGARRKEPRVLRYMLCSMTQRALIAPSVWIAVSLMHGESLLCACSAAADLRRFFNRSRAAPPERELARLLARIPCENVFDGHELISREAASRYIRCMSQACGWTFLLIITLVAFMVRGIRPCFTQAAFLKSKYWSHYIDTERRLFDETCREHAKSFAKVCIQQYFESISGEIMVFHNHHSQKGENLKHNKEETHTSEEEKLLGIREKDYMNKVLWNWHTCKPPLHLHKRGKEESVSEHSGVHAHSNDSHSQKSVKNGYVAYYSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KT28</accession>
    <name>A0AAR2KT28_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Serine/threonine-protein kinase 24</fullName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Mammalian STE20-like protein kinase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">STE20-like kinase MST3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Monomer. Interacts with CTTNBP2NL. Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner. Interacts with PDCD10; this interaction is required for the association of STK24 with RIPOR1. Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KT28"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045284.1">
        <property type="protein sequence ID" value="ENSPNAP00000065286.1"/>
        <property type="gene ID" value="ENSPNAG00000016531.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153476"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048679">
        <property type="term" value="P:regulation of axon regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000411">
        <property type="entry name" value="Probable Ste20-like kinase Don3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.70:FF:000002">
        <property type="entry name" value="Serine/threonine kinase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000252">
        <property type="entry name" value="Serine/threonine-protein kinase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="18 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="30" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="32">
        <location>
            <begin position="23"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="293"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="30">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="31">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00066233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00072153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00077261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00079554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="430" mass="48203" checksum="7AEB888702067117" modified="2024-11-27" version="1">RNHRTVQGRRCQKNLKADPEQLFTKLERIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPFITKYYGSYLKGTKLWIIMEYLGGGSALDLMEPGALDETQIATILREILKGLEYLHSEKKIHRDIKAANVLLSEQGEVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGEPPHSDLHPMKVLFLIPKNNPPTLEGNYSKPLKEFVDACLNKEPSFRPTAKELLKHKLIVRHAKKTSYLTELIDRYKQWKTEQSREESSSDESDSEPDGQASGGNDFGNDEWIFTIREKDPKKLQNGASLAGEQVQNKRPLSQSLSTIITPVLTELKERGGDAGVKPDVLDELGEAIFLAEEAYPGISDVMVTQLIQRLQRVGETSGNTKNKNMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KXC3</accession>
    <name>A0AAR2KXC3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Bromodomain-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">BRD8</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 8 ISOFORM X1"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </location>
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    </evidence>
    <sequence length="582" mass="65131" checksum="A9A109DFA4571D50" modified="2024-11-27" version="1">MEVEDPITAKFQTLVESLMAKTTSEIVKVFAEVLLEIRLEISQSWSGIDELKLEESEEQNTEDVFRSEATVNFKREMEEVLVSDDVVQMPKVTIIENTCITQSSVVVHESETSQTTPSDTSDNNQSVNSPSQTQVGQVSQPVVKRKRGRPAKSSRVTVHQKSMVEVVSKSPLQAISMVSPLQPSCSSSTVASASSNTEALALNTDFLAAEDIQDEETAVDEKGIPGFHHVERLAEYLVELRNLSTMTLTNQQASTIIDLWQNLDTWDKQRIVSVAQNQGRLLTSHFQSTEKETTFTPGLESTTKCDLGASVSSAQRPDCCRLLEAIFVRLCDIYFSPQKKCEGMLTQWTLILQDYTKIRGLVLENSAVMQGTSLQLVDVNQNSLIQWHNHRIKDHGLTVLLKEDHAPSLPELEDPLVQTRLLPVEPTQHQTLSHEYIPPEGLAGHPRLKGRDIGEPPAIRPKLSTQRELYEPAPKPAQYLQVVPHISVRAAPVLQALHVLQPMPTFVCAPPQNTIQQCATQDPPQPRQKRAYNRVVQGNTCKKCGQFRSAVTGHSQYKGIVYCPQTETLDKKQWLEKMRKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KZK4</accession>
    <name>A0AAR2KZK4_PYGNA</name>
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        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="4" type="primary">PCBP4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017568803.1">
        <property type="nucleotide sequence ID" value="XM_017713314.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KZK4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057444.1">
        <property type="protein sequence ID" value="ENSPNAP00000067914.1"/>
        <property type="gene ID" value="ENSPNAG00000035500.1"/>
    </dbReference>
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    <dbReference type="CTD" id="57060"/>
    <dbReference type="GeneTree" id="ENSGT00940000159711"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22517">
        <property type="entry name" value="KH-I_PCBP4_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22520">
        <property type="entry name" value="KH-I_PCBP4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22523">
        <property type="entry name" value="KH-I_PCBP4_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000003">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="25"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="109"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="278"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="464" mass="48126" checksum="ADD7727B43CAFF9B" modified="2024-11-27" version="1">MRLPGPDSTARMNCEQDFGDGGMNVTLTLRLLMHGKEVGSIIGKKGETVKRIREESSARINISEGSCPERIITITGPTECVFRAFTMITIKLEEDLTALVANGTVTSKPPVTLRLVIPASQCGSLIGKGGSKIKEIRESTGAQVQVAGDLLPNSTERGVTISGSQEAIIQCVKLICTVILESPPKGATIPYRPSPSPGAVLLTGNQVFEASEFGSHPLFSVAQGALDLQQAYTVQNQYGIPHSELAKLHQLSMQQGLAPIAQPAATPVLPAGMDSNSQTASQELLIPNDLIGSIIGRQGTKINEIRQVSGAQIKIGSQLDSTSDRHVTITGSPISINLAQYLITSCLETAKSTAQSSSMSTPVDLNMSFTQPASPASSSAAALAAMGGLPHAPILGAPYALPLSSLLGMKSVPFLALSAPAATAAAAAAAAAAAPAHGTLASYTAKISSANGIKKPERQKFAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L2B7</accession>
    <name>A0AAR2L2B7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L2B7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047352.1">
        <property type="protein sequence ID" value="ENSPNAP00000068521.1"/>
        <property type="gene ID" value="ENSPNAG00000014486.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154856"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF839">
        <property type="entry name" value="HATCHING ENZYME 1.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="42"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="254" mass="28976" checksum="8DA8F373ED190D76" modified="2024-11-27" version="1">MFLVISRARMEREAVGSMLLQCVEIWMLLHRSSVSKPCEIWSFIHMDTHRLTIILIANLPDKTGKKCFWKKSSNGLLLVPFTVSNDFCLSLPATFHSKTCIRFVSRTLEADYLSIQSRAFRSALLLCYSPVGRQGGVQVVSLSRNGCVYHGIAQHELNHALGFYQEHTRSDRDYYVRINWANISPTLQYNFNKENTNNLNTPYDYSSVMHYGKTAFSMNGQDTITPIPDPSVPIGQRVDLSAIDIKRINILYNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L3Q6</accession>
    <name>A0AAR2L3Q6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">V-type proton ATPase 21 kDa proteolipid subunit c''</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Vacuolar proton pump 21 kDa proteolipid subunit c''</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ATP6V0B</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR. Interacts with IFITM3. Interacts with TM4SF19; this interaction inhibits V1-V0 complex assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasmic vesicle</location>
            <location evidence="8">Clathrin-coated vesicle membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558193.1">
        <property type="nucleotide sequence ID" value="XM_017702704.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L3Q6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073585.1">
        <property type="protein sequence ID" value="ENSPNAP00000069354.1"/>
        <property type="gene ID" value="ENSPNAG00000037259.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430296"/>
    <dbReference type="CTD" id="533"/>
    <dbReference type="GeneTree" id="ENSGT00550000075120"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18177">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18178">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000002">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="14">
        <location>
            <begin position="53"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="14">
        <location>
            <begin position="139"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="206" mass="21591" checksum="3758389E14C4F8FD" modified="2024-11-27" version="1">MMNGHAILYTGVTLAFWSTILIVGICYTIFDLGFRFDIAWFLTETSPFMWANLGIGLAISLSVVGAAWGIYITGSSIIGGGVKAPRIKTKNLVSIIFCEAVAIYGIIMAIVISNMAENFSGTTPDTIGARNYQAGYSMFGAGLTVGFSNLFCGICVGIVGSGAALADAQNASLFVKILIVEIFGSAIGLFGVIVAILQTSKVKMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L6G8</accession>
    <name>A0AAR2L6G8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 24">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="6 24">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21 24">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">KARS1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 24">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="6 24"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L6G8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044462.1">
        <property type="protein sequence ID" value="ENSPNAP00000072140.1"/>
        <property type="gene ID" value="ENSPNAG00000013828.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234618"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003877">
        <property type="term" value="F:ATP:ADP adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002276">
        <property type="term" value="P:basophil activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015966">
        <property type="term" value="P:diadenosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043032">
        <property type="term" value="P:positive regulation of macrophage activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000050">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000029">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="26">
        <location>
            <begin position="265"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00059215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="612" mass="70075" checksum="B5ADF71CAFFB5383" modified="2024-11-27" version="1">PIFHVSIKLVDGFKCQFAWLTVSANHSRKRSARLTASHTACSFCGFSELKRRMKAEKKAADKEAKVKDQVQQNKETNDTPQQNAYAADEESLDPNQYFKIRTQAIQALKGTAEDPYPHKFHVDLSLTDFIEKYNHLQPGDQLTDVVLTVAGRVHAKRASGAKLLFYDLRGEGVKLQVMANSRNYKSEEDFVHINNKLRRGDIIGVRGNPGKTKKGELSIIPIEMTLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFVTRSKIITYLRSFLDQLGFLEVETPMMNLIPGGAVARPFVTYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEMTEKLLSGMVKQITGGYKVTYHPDGPEGQAYEVDFTPPFRRISMTHDLEKEMGVKFPAPETYDSNETRKFFDDLCVQKGVECPPPRTTARLLDKLVGDFLEVTCINPTFICDHPQIMSPLAKWWVHRSQKGLTERFELFVMKKEICNAYTELNDPIRQRELFEQQAKAKAEGDDEAMFVDETFCSALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLFFPAMKPDDNKPVPPAEGTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJY5</accession>
    <name>A0AAR2LJY5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR5 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037392001.1">
        <property type="nucleotide sequence ID" value="XM_037536104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJY5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046126.1">
        <property type="protein sequence ID" value="ENSPNAP00000076873.1"/>
        <property type="gene ID" value="ENSPNAG00000033107.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108414363"/>
    <dbReference type="CTD" id="83916"/>
    <dbReference type="GeneTree" id="ENSGT00940000153391"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06944">
        <property type="entry name" value="NR_LBD_Ftz-F1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000011">
        <property type="entry name" value="Nuclear receptor subfamily 5, group A, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF48">
        <property type="entry name" value="FF1D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002530">
        <property type="entry name" value="Nuc_orph_FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="25"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="241"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="362"/>
            <end position="365"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR002530-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="485" mass="54400" checksum="72A578BB942F1026" modified="2024-11-27" version="1">MLEAESKVCEGLLKGMEFPNSDEFEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKRYTCSQNQDCGIDKTQRKRCPYCRFQKCLSVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKRALIRASGFKLESNPPLVPSAQADYPFAGSLPSLLPPSLAPPEYDCPPLCPPALGVALQSYGSFPAQYQYTTPTLPGRSIKAEHPDPYSTSPDSSLAYPYTEGCLPASPQTSPMNPTVPSLVLELLSCEPDEEQVRGKICAYLQQEQSGRGKLDKPRPFNLLCVMADQTLFSIVEWARSCIFFKELKVGDQMRLLHNCWSELLLLDHICRQVHHGRENSLLLITGQEVELAGVASESGLTLSSLVQRGQELSHRLQLLQVDRREMACLKFLILFNPNVKLLENQAFVESVQEQVNGALLEYTLSTYPQHLDRFSQLLLRLPELRALSNQAEDYLSSKHLSGDVPCNSLLIEMLHAKRATAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LK73</accession>
    <name>A0AAR2LK73_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SEMA3E</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580187.1">
        <property type="nucleotide sequence ID" value="XM_017724698.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LK73"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045307.1">
        <property type="protein sequence ID" value="ENSPNAP00000076950.1"/>
        <property type="gene ID" value="ENSPNAG00000022177.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443748"/>
    <dbReference type="CTD" id="9723"/>
    <dbReference type="GeneTree" id="ENSGT00940000158437"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041416">
        <property type="entry name" value="IL-1RAcP-like_ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF22">
        <property type="entry name" value="SEMAPHORIN-3E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18452">
        <property type="entry name" value="Ig_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sema" evidence="14">
        <location>
            <begin position="48"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="564"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="761"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="776"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="787" mass="90033" checksum="E4114F50F68ACAA0" modified="2024-11-27" version="1">MVETESKRHFRTSACARMAGCVWTLTLILCVVLWSDFEVQGAKNNYPRLRLSHKELWDLNRTWVFQAGEGVLQHYSMLLDETQERLIIGGKDILYSLNLDRVSAPHSEIHWASSEEQVEQCLMQGREKPECANYIKLIQQYNSTHLLTCGTGAFSPACAYIRVSHSMENRVFQLELEHIESGRGKSPFHPNSPTISTVSRGQLFVGLYTDYWENDAALYRLGNHGYTRTEVGDRQQLNEPKFVGSVAIPDNDDPADDKVYFFFTERVPNTEGGHKAVYSRVARVCANDHGGQRMLVNRWSSFLKARLICSVAGPNGIDTHFDELEDVFVLKKKDEKNPEIFGLFSTTSAVFKGYAVCMYNMEEIRAVFNGPFAHRERADHHWSVYEGKVPYPRPGSCASKINGGQFSSSKEYPDEVLRFVRSHPLMFQPVQPVHRRPILLDTDGRRRLTELAVDRVEAEDGHYNVLFIGTDDSVVLKVITIFNKEANTVEEVLLEELQVFKVPVPITEIIISTKRQQLYVGSEVGVAQVRVHQCGLYGSACADCCLARDPYCAWDGHTCSRYYPAGIYTKRRFRRQDVRHGNAVQQCNGLQLNDAQSVLEKLVYGVEKNSTLLECRPRSLQALLTWYMLKGLDMEEIKGDEHIIQTSHGLLFLTTKKSDAGVYVCQSEEHGFVQTIVRVTLEVLEEERVESLFHRGEYEEKEVQFRSPPCPFPSLPSAPSATKLWYKDFMQLIGYSNFQRVEQYCERVWCASDRKRKKLKGLPPKWRYSQGAERHGKARAPRHTRDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LN47</accession>
    <name>A0AAR2LN47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial</fullName>
            <ecNumber evidence="4">1.3.1.124</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2,4-dienoyl-CoA reductase [NADPH]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DECR1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in mitochondria. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2E,4E)-hexadienoyl-CoA + NADPH + H(+) = (3E)-hexenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84788"/>
            <dbReference type="ChEBI" id="CHEBI:84790"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LN47"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046454.1">
        <property type="protein sequence ID" value="ENSPNAP00000076174.1"/>
        <property type="gene ID" value="ENSPNAG00000030672.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153801"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000288">
        <property type="entry name" value="2,4-dienoyl-CoA reductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658:SF14">
        <property type="entry name" value="2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="375" mass="40367" checksum="04911906761C83C1" modified="2024-11-27" version="1">MWPSDQLKNSIQSFMECVSLAEKLHPSLRSPSAMQSVKYRGVKHHHWTLEQSLGLAVARRTLFGNGTAVLRKSDDDRAPQARFFPPLQEPMLLPGTFKNKVAFITGGGTGLGKGMTFTLSSLGAECVIASRNLDVLKATAEEISSQTGNKVHPVQCNVRDPDSVKATVDQMVNDVGLPDVVINNAAGNFISPSERLSANAWKAITDTVLNGTAYITLDIGKRLIAAQKGAAFLCITTIYADSGSGFVMPSASAKAGVEALCKSLVAEWGRYGLRFNIIQPGPIKTKGAFSRLDPTGKFEKLMIERIPVGRLGTVGEIANLAAYLCSDYASWVSGATIKIDGGEYSFMAGEFNSLSLVTKEQWDVMEAIIRQTKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LRM0</accession>
    <name>A0AAR2LRM0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neuritin 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuritin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559428.1">
        <property type="nucleotide sequence ID" value="XM_017703939.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LRM0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080744.1">
        <property type="protein sequence ID" value="ENSPNAP00000076956.1"/>
        <property type="gene ID" value="ENSPNAG00000031419.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108431051"/>
    <dbReference type="CTD" id="436780"/>
    <dbReference type="GeneTree" id="ENSGT00530000063853"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990138">
        <property type="term" value="P:neuron projection extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026144">
        <property type="entry name" value="Neuritin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902:SF1">
        <property type="entry name" value="NEURITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902">
        <property type="entry name" value="NEURITIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15056">
        <property type="entry name" value="NRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043781418" description="Neuritin 1b" evidence="9">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15429" checksum="03F691B83004458D" modified="2024-11-27" version="1">MGLTLSGRYISLFLAVQIAYLLQAVGAAGKCDTVFKGFSNCLLQLGDNMAQYPQELDEKKNLETICMYWDDFHSCATTALADCQDGATELWEKLKKESRSLQFRGSLFELCAGGNGAPRAGPPLSLSLLLPALSALLTSLCAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LU34</accession>
    <name>A0AAR2LU34_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Piezo-type mechanosensitive ion channel component</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PIEZO (TC 1.A.75) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037390494.1">
        <property type="nucleotide sequence ID" value="XM_037534597.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390495.1">
        <property type="nucleotide sequence ID" value="XM_037534598.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390496.1">
        <property type="nucleotide sequence ID" value="XM_037534599.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390497.1">
        <property type="nucleotide sequence ID" value="XM_037534600.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000087839.1">
        <property type="protein sequence ID" value="ENSPNAP00000078249.1"/>
        <property type="gene ID" value="ENSPNAG00000021501.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442287"/>
    <dbReference type="CTD" id="9780"/>
    <dbReference type="GeneTree" id="ENSGT00940000157348"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031334">
        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056770">
        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056769">
        <property type="entry name" value="Piezo_TM1-24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056768">
        <property type="entry name" value="THU_Piezo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF5">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12166">
        <property type="entry name" value="Piezo_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24874">
        <property type="entry name" value="Piezo_THU9_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24871">
        <property type="entry name" value="Piezo_TM1-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23188">
        <property type="entry name" value="THU_Piezo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="84"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="118"/>
            <end position="139"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="206"/>
            <end position="226"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="277"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="321"/>
            <end position="341"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="482"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="528"/>
            <end position="549"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="596"/>
            <end position="613"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="619"/>
            <end position="639"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="646"/>
            <end position="664"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="697"/>
            <end position="718"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="787"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="813"/>
            <end position="838"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="850"/>
            <end position="869"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1008"/>
            <end position="1026"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1038"/>
            <end position="1060"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1160"/>
            <end position="1178"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1184"/>
            <end position="1203"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1215"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1673"/>
            <end position="1696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1702"/>
            <end position="1723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1730"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1966"/>
            <end position="1989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2009"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2037"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2070"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2179"/>
            <end position="2203"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="2434"/>
            <end position="2457"/>
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        <location>
            <begin position="26"/>
            <end position="724"/>
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        <location>
            <begin position="1133"/>
            <end position="1459"/>
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    <feature type="domain" description="Piezo transmembrane helical unit" evidence="14">
        <location>
            <begin position="1686"/>
            <end position="1808"/>
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    <feature type="domain" description="Piezo THU9 and anchor" evidence="16">
        <location>
            <begin position="1964"/>
            <end position="2201"/>
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    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="12">
        <location>
            <begin position="2238"/>
            <end position="2521"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="150"/>
            <end position="189"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="389"/>
            <end position="426"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1344"/>
            <end position="1395"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1457"/>
            <end position="1507"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1812"/>
            <end position="1831"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1840"/>
            <end position="1921"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="151"/>
            <end position="161"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="162"/>
            <end position="177"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1352"/>
            <end position="1390"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1457"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
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            <begin position="1492"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="1813"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1850"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
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    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1908"/>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007821"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023065"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
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            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
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            <dbReference type="Pfam" id="PF15917"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
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            <dbReference type="Pfam" id="PF23188"/>
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    <evidence type="ECO:0000259" key="15">
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            <dbReference type="Pfam" id="PF24871"/>
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    <evidence type="ECO:0000259" key="16">
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            <dbReference type="Pfam" id="PF24874"/>
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    <evidence type="ECO:0000313" key="17">
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            <dbReference type="Ensembl" id="ENSPNAP00000078249.1"/>
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    <evidence type="ECO:0000313" key="18">
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            <dbReference type="Proteomes" id="UP001501920"/>
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    <evidence type="ECO:0008006" key="19">
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            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="2525" mass="289961" checksum="2FA374B2EA054447" modified="2024-11-27" version="1">MELQLGCGLLYCFLLPAFLLAACLFRYNALSLVYLLYLLLLPWFLWPNKHTLRGHTGRFIKALFSTSLLFLLAHVCFQICLYTVPDLDYALGHNCSSWDTLARHIGMSRLPLDKPWTVVRLLAPDLGIFIISLITMVLCNRLVKSREEGTTAHSSTLNQEEAGTDEEEEEEEDEEEDGRQSSSAPEEESSRADLLAARLRAAAQRFLRNVGRLLAVTLLALAGITLPSAFSALYFLLFIGVCTWWACHFPISQLGFNALCVMVSFFSAGHLVCLYLYQSTFAQSIFTPSSLWARLFGLKDIIKPTNCSSFGDLILNTEHDWPVYVNPGILLLLYITVTTVLKISSHSYTETIVGEAKVTESGNGEEVELRPWGARKSTGDDTKQMLLATGESPGQESDPPNTKEHVTNGSSQHELGIDGEAPEKQEEDSPLRLVGKMIMQQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWMLRARRRFAMLCSPFILLYGLGLCGLQYVWAMDLEPELPQNVGYMSLRQMGLDRAYYPCLRLGAMLLFTLTFWLLLRHSVKDIFCKKKQAAPLQEVTTGENAGRDDSILKLLGRVVMSLYAKYWIYVCGGMFIMVSFAGKLVAYKIIYMLLFLLCMCLYQVYYSLWRRLLKPFWWLVVAYTMLVLIAIYTFQFEDFPGYWKNFTSFTEEQLADIGLETFKLSELFTSIMIPGFFLLACILQLHYFHKPFMKITDLEHVTPLNRKRSVPKPEVLQSSEGGGFEEEDLHTELGDDESEDGVMVPSKWGLVMDRLMVLFRKFSDILTLIQAFIWRILELHILKIVAFFVVWVALLEPSVMNLVLVVLWSFAMPYARFRPMASCLSTVWVCVIIVCKMLYQLSIVNPNEYSSNCIVPLPNETSLESNEILNSTLYKEPVDPANWFGFRKDATALGYSKNHLLVLLLLVFEATVYRHQVHHYKQLQRSPPTIPAIFPQATRDKLDQGLLPCIKYLLNYSFYKFGLEICFLMTVNVIGQRMNFLVIMHGCWLVAIMVRRRRAAIAKIWSKYCLFLVIFMIYQYILCVGIPPVLCIDYPWRWKTSLTINSALIKWIYLPDFYTIPNSKNLIADFLLLMCASQQWKVFENEKKEEWMVMGGENNDNPDPMEGIPFNPAPNFINCRSYLDMAKVLVFRYMFWFVLAVVFVTGSTRISVFGLGYLLACFFFLLFGTQLLIKRSRTRLLMWDCLIIYNVAVIISKNILSILACVFVAEMQSRFCWVIQLFSLVCTVKGYYDPESLSDKDCSLPVEEAGIIWDSICFFFLLLQRRVFLSFYFLHVTADLQASARQASRGFELFRASIIKNMRFHQQAEKKSIQQLKSSMQRIRDKQEKYKEGRKASETEEHPSELQEERTERKEKKEGKSSSQKEWYRPWIDHATVLHSGDYYLFESDSEEEEDVSVEEQKQRRQTAFQLAYQAWVRSAKQALRERQQRQREQRLERERQQASSRASGDGEEAGAGVSQVEEQELGEEAEESGTGSDTVQRILDILKFLWVLCLAMVDGLTLWLNMLTKQYVDTSTVLCDERYMLTHNISQMPSREPMDDQLSQGSESPTLETCLDETDTDVVNVSGAVSAAGLEEISELDGAQGCSSTTLSSQPTLQLEAESRAYRRQRRSRTASELLSDRPYCIEELVQSREFYSTQNRLLKLLFALYNVLAAHSELVCYFIIVLNNVVTASVISLVLPILTFLWAMLAVPRPSKSFWMTAIVYTEVMVVVKYLFQFGFYPWNSVRVTNMNKNDPFFIPRILGLEKTDNYIRYDLLQLLALFFHRSLLQRYGLWDQEGPLEEKASPSSEQEKAKKEGEEMDAGVFELDDKTPEPDQEAQTQTDTQTETQTDPEQSQSAEITPEPPSPHKEQGKRKGLLRFRKRKPSNKDTTAKETKKKKKAKAKRREEASKKLKAFGEKLKGFFVSVVRNVYRPPYRFFQNILHAEYRAATDVYALMFLTDVVDFIIIVFGFWAFGKHSAATDIASTLSEDQVPEAFLVMLLIQFSTMVIDRALYLRKAMLGKLIFQVILVFGIHLWMFFILPAVTERMFSQNFVAQLWYFVKCIYFTLSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIVFFLICIIWFPLLFISLVRSVVGVVNHPVDVTVTVKLGGYEPLFTMSVQQQSIQPYTQEQYVSLTKWFGDNAVAMQFITLYSYEDIVTANIEGSSGSVWRISPPSRQELIKELLGSADMTLRLAWNFQRDLSKGGTVENTFDKHSINLERGNPVRADLALLLEGNRTEPVLVPHMFPNFIRAPNGAEAKPVSQLYEGNEEGYQNVTLSLKRDGSLEWWDISIAGCNTSSVACKVLRMDIFNDKVSPPSLGFLAGYGIMGLYVSVVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCTDIFLVRETGELELEEELYSKLIFLYRSPETMIKWTREKNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M516</accession>
    <name>A0AAR2M516_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar fusion protein MON1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an important role in membrane trafficking through the secretory apparatus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the MON1/SAND family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M516"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081908.1">
        <property type="protein sequence ID" value="ENSPNAP00000082031.1"/>
        <property type="gene ID" value="ENSPNAG00000014681.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006665"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF13">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="4">
        <location>
            <begin position="93"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="5">
        <location>
            <begin position="255"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="6">
        <location>
            <begin position="383"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="502"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="502"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="532" mass="60202" checksum="CB9DDCD544357FAD" modified="2024-11-27" version="1">MVILRRVAGLSLRDRVRSSVIREGLGVELLLLHVERSQVRWFGHLVRMSPGPLTREVSQARQPGRRPRGRPRTRWPGSASESPPGSYWRSRRKHVFVLSEAGKPIYSHYGSEEALSSVMGVMMALVSFVQVGGNTIQSIHSDGHIIVFLQKGPLVLVSASKSHQSELQLRSELLHVYHQIVSMLTQASINRIFQCRKNYDLRRLLFGSERVLDALLDVMETEAGFLLSAVQCLPLAPSTRDTISQILQKAITPNLVLSILLYNGQLVSIVQEKMVIEDARLKSTDLHLLLNLIRASSAFQTGEIWTPICLPCFNPDCYFYTYVAYLDPPDCTICLVLLSTDKDAFYAVAECKRKIEEGVRTHNALQCISKVQLCQVCHVGVAELRHFLFKPLDVPDHHSQLPQFTSPEVEAPYSTEEKRIYLMDLYWHLHSRIHSTLRPIKLLYHATQRETLLALVTGKFEMYACFSPLVTKSTAIGAVTKLLRWIKKEEDCLFIRSPPKYSTTPPEARGFSNSRVQTNKQDKSDSSVLSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRF3</accession>
    <name>A0A3B4BRF3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein 178B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TMEM178B</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM178 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551332.1">
        <property type="nucleotide sequence ID" value="XM_017695843.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRF3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010820.2">
        <property type="protein sequence ID" value="ENSPNAP00000002218.1"/>
        <property type="gene ID" value="ENSPNAG00000001136.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426399"/>
    <dbReference type="CTD" id="436669"/>
    <dbReference type="GeneTree" id="ENSGT00390000015299"/>
    <dbReference type="OMA" id="RQHNSTN"/>
    <dbReference type="OrthoDB" id="8809386at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039625">
        <property type="entry name" value="T178A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017386349" description="Transmembrane protein 178B" evidence="8">
        <location>
            <begin position="29"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33493" checksum="1A2DB0E929A071C4" modified="2018-12-05" version="1">MAAGKLLLYAGLSLSLCALGMLAVAVCSDHWYETDARRYRERCRSFASRRKDPGFIYIPNNSLPLRASAGGRARLDRWEERLLLARNRRQLFAMSAADECSRQYNSTNMGLWSKCHRLGFDQDIEDLIRKGSITRCSYIKYHYSSATIPKNLSYNITKTIRQDEWHSLHLRRMTAGFMGMAVAIILFGWIIGMLGCCWDRGLMQYVAGLLFLMGGTFCIISLCTCVAGINFELSRYPRYLYGLPDDISHGYGWSMFCAWGGLGLSLIAGFFCTLAPSVQPLPRSTCPKSRQENGTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRP5</accession>
    <name>A0A3B4BRP5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dendritic cell-specific transmembrane protein-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DCST2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRP5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001119"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012697.2">
        <property type="protein sequence ID" value="ENSPNAP00000001119.2"/>
        <property type="gene ID" value="ENSPNAG00000007967.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153269"/>
    <dbReference type="OMA" id="DAKDNCM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051856">
        <property type="entry name" value="CSR-E3_Ligase_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012858">
        <property type="entry name" value="DC_STAMP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058842">
        <property type="entry name" value="DCST1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041:SF6">
        <property type="entry name" value="DC-STAMP DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041">
        <property type="entry name" value="DENDRITIC CELL-SPECIFIC TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07782">
        <property type="entry name" value="DC_STAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26039">
        <property type="entry name" value="Dcst2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26037">
        <property type="entry name" value="zf-RING_DCST1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Dendritic cell-specific transmembrane protein-like" evidence="6">
        <location>
            <begin position="356"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase DCST1-like C-terminal" evidence="7">
        <location>
            <begin position="599"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001119.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="677" mass="75949" checksum="046B636BBADE1E4A" modified="2024-11-27" version="2">MIQEEIFTPVGRCLSWLLGSSAMQQARCSLVGFFIGLLLTSVYGMTALYIQNYSLWYCLITTTTIAVFTAFGMGLSIRVRTNVILMLPMLCSKEGKRFLLFLILTLLVQGPLSNTVENFERAADSVVCGAELAMNQTQLLMQRAATPLLSVLSKIKEVSRNVFSLSGRVQNFITALTESVRHVSGTLRNVMHFLASIGDICNDKMGTPYKKCNALFDEGRADCMKLLSIFSFLCHIVDGFRPLCGLTRVGILFCILPSYIAEHLKTRLAFPTIAAFDQMKKEFEFNMSASVHFDMNLNRSQSMQEMAQEIMEEVALEQERFLELMAVLAYVGLFLLLFMYLQAVLYKKRYLHTDDFDNIYITEQFVEMDSKLSRQGRPAVLPLREREALTYITPYSLRMTARERQAVAAGLVTLLQHMAVGCVVVALDLMVFWVFDMLHHQAQGEIVARAPVVVDVQVNGSGYASDIFKDIVASFDILQRGNITILSKKCLMEPLELDYISYLFIGFLYGLTFFLVLAGGYAKRLQRSVCAHYHPQREKERILWLRGHILSQRGSLGKALLRAVARGKEDQGHGSVLQILTLRLPAGATIARFLGVFNKSCLACGTVMKSKDCPDVYCCSTPNCTGCFCMQCFRVMGRICAVCMGPLTFQEDSEQELDSSDEEQVNLWAAAITTPHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSH1</accession>
    <name>A0A3B4BSH1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSPNAT00000012116.2">
        <property type="protein sequence ID" value="ENSPNAP00000001470.2"/>
        <property type="gene ID" value="ENSPNAG00000008181.2"/>
    </dbReference>
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        <property type="entry name" value="TM_EGFR-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
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            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
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            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="343"/>
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        <location>
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            <end position="484"/>
        </location>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="484" mass="53253" checksum="352CAA08F67FD26C" modified="2024-11-27" version="2">MSTRDHAQITQELRSEDGAPSSKWPSTILQDHHITGESHTEHKITETQIITEEKKSTSHLSSLGQLTKRSSDYTSLHEDHGPSTRGQEPTVISTRNHDEDTTGLRPEDSVLSSKMHSQITQDQQITGMSHTENKTTEVQITVGESESPSDFSSSASSKGHTKRSLDYSQNSQTTSTPTEQTTLMSQTNITTGDQHQTSHIFKSTNKTGVTDNQLQEISRSTVKIFNLETNHTNFQTSEVITYEPRKVSAVPQTNMTTKWSKQTQGTEGTKASPKKQNKNNAGPIVAALIGSVLVLMLIAIVVILAKRHRMHKKKLENPDWAGPSPFLEGDTQHGNGEDGPFQRRDTKRISLHSFFPQRLSQRLSLLVEEEVQMNEIAVSSTFGKNEQPQNGKPAEEQEQIQTQKTANQVPAPEASADSTVTEMVTGPAVPDNNANIKPTSKPDDETIPSPSSQTNTVPPRPYEDVDLNPPQTNAVDTPPAPPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BX34</accession>
    <name>A0A3B4BX34_PYGNA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MYG1</name>
    </gene>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MYG1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017719170.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000003080"/>
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        <property type="gene ID" value="ENSPNAG00000009469.2"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215:SF1">
        <property type="entry name" value="MYG1 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03690">
        <property type="entry name" value="MYG1_exonuc"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00010105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="381" mass="43593" checksum="503AE41943608A04" modified="2018-12-05" version="1">MRRIPSFKSCRFFEPYFYQIARGTPFYPTATEGSHLFITRRQYMHSAKRPCLQEMSSVKIGTHNGTFHCDEVLACYFLRQLPEYKDAKITRTREPAELAQCEVVVDVGGVYDPSQQRYDHHQRSFVESFNSLCPEKPWVTKLSSAGLVYFHFGRRILSQLTGLTQDSRQLEILFDKLYENFVEEVDAVDNGISQCDGEARYTISTNLSARVGYLNPRWNSDCQDTEEGFLKAMVLVGTEFLDRLDYYQNAWLPARAVVEAAVQTRHQVDPSGEIIMLAQGGCPWKEHLFALEKELKVEVPIKFVLYPDQNGQWRVQCVPKGLNTFQNRLSLLEEWRGIRDNALSQLSGIPDCIFVHSSGFIGGNKTKEGVIEMAKRTLLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BY67</accession>
    <name>A0A3B4BY67_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 10">Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017564722.1">
        <property type="nucleotide sequence ID" value="XM_017709233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BY67"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003495"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008633.2">
        <property type="protein sequence ID" value="ENSPNAP00000003495.1"/>
        <property type="gene ID" value="ENSPNAG00000009900.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000155285"/>
    <dbReference type="OMA" id="ECIRSMQ"/>
    <dbReference type="OrthoDB" id="5964374at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="bHLHzip_c-Myc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myc proto-oncogene protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003327">
        <property type="entry name" value="Myc-LZ"/>
    </dbReference>
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        <property type="entry name" value="Myc_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002418">
        <property type="entry name" value="Tscrpt_reg_Myc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012682">
        <property type="entry name" value="Tscrpt_reg_Myc_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45851">
        <property type="entry name" value="MYC PROTO-ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02344">
        <property type="entry name" value="Myc-LZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01056">
        <property type="entry name" value="Myc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001705">
        <property type="entry name" value="Myc_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00044">
        <property type="entry name" value="LEUZIPPRMYC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="351"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="219"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="327"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="436" mass="49201" checksum="C344AD3A75728336" modified="2018-12-05" version="1">MVPGTTGPLEHGRILDFTRAKCVSCAVFAHSDGVFVDTCPRAIMPMSASLACKNYDYDYDSFQPYFYYDEDEDFYQQHRQPPAPSEDIWKKFELLPTPPLSPSRRPSTADQLEMVTELLGDDAVSQSFICDMDPSQALLGSIVLHDCMWSGFSAAAKLERAVSERLASLRAARRDTALLERGGPTRLSAAATYLHDLGTPASECIDPSVVFPYPLTESPRAAEVTPDTPPNSSSGSGSDSEDEEEEEEEEEEEEEEEEEEDDEEIDVVTVEKRQRRSEVEACETRQLSPLVLKRCHVSTHQHNYAAHPSTRHEQPAVKRARLEAASLAGGGRHGKQRKCASPRTSDSEDNDKRRTHNVLERQRRNELKLSFFALRDEIPEVANNEKAAKVVILKKATECIRSMQSDEQRLLSVKEQLRRKSELLKHRLQQLRRSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYT5</accession>
    <name>A0A3B4BYT5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">IL12RB2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576733.1">
        <property type="nucleotide sequence ID" value="XM_017721244.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYT5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004748"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006504.2">
        <property type="protein sequence ID" value="ENSPNAP00000004748.1"/>
        <property type="gene ID" value="ENSPNAG00000011135.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441618"/>
    <dbReference type="CTD" id="3595"/>
    <dbReference type="GeneTree" id="ENSGT00940000155776"/>
    <dbReference type="OMA" id="KWAKECT"/>
    <dbReference type="OrthoDB" id="10005435at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF2">
        <property type="entry name" value="INTERLEUKIN-12 RECEPTOR SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="601"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="219"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="408"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="499"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="813" mass="89985" checksum="DA65FF6A828EFDDB" modified="2018-12-05" version="1">MVEVDVQLEQVGSMPLKCSIWPNFIFLLVLATGAPVGSKGADEKICSIHSSKEVHMGSSFQVYCIFKKECKTLIFRDNVPLQCTSCNTTHVSLSIVNVTRATTLACRCEGNPEPCGTDITPGYPPEIPQNLICVQEGELGNVKCTWKAGRETYIPTTSRLWVGGDPVDYESGTLPDGTLSASFPIPGAQTNFSVLVNASNSLGSATSGVLSFMLNDIVKPLSPNIKKVECTSRQCQLYPDNAQSIQLVEIQYRADEGSWNTVSFNHTSSNTSWTITSLNPYSLYTFQVRWKLGPTRGVWSAWSRAEKLTDEEAPAAMVDAWYIEETTEKVKKNIHLFWKELSKSDARGNILGYNVSVVDQGKKICNTFITPPDRSYRVPCSVCNVTISAVNSKGQSSARLIQLQPIVSADNFSSIKRIDNHSIALSWTSEGTVKEYLVEWYPAGCKQQLQWIRVEHHLNTIHITGLQPAECYDGAVIYFRPSGTSKAVFSNIATWPSAPQQGPNCDTMVKYERVEVTWQEVPSEKRGGCLIRQYRIYLQEPGGKIKNYNVSHPQRQYTIKGLAQGQPYKLWISAVTTAEGPKGFECHFVTHQGVPPAEKPLALVLSIGSAIFLTCLFLLCICQFSSVHRRLSRCCHCLMPSIVPDPANSKWAKECANEKGEMKLQLYLSDSSMSEEEPDTVEVQELPQEKLLQGETVPIDGACPSVSNFEVQAQEHHIPSSPYQPITTSSYLKSLSNSSDTTQASRNTDITVDYISTHGVLSGEEDDEEEEMDMMGFFPCPTSPFLDPLMPTGGKLTLDIVKIDCSDLNLCIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0V5</accession>
    <name>A0A3B4C0V5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Cleavage and polyadenylation specificity factor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 8">Cleavage and polyadenylation specificity factor 100 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CPSF2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551173.1">
        <property type="nucleotide sequence ID" value="XM_017695684.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0V5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005408"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005366.2">
        <property type="protein sequence ID" value="ENSPNAP00000005408.1"/>
        <property type="gene ID" value="ENSPNAG00000001849.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426305"/>
    <dbReference type="CTD" id="53981"/>
    <dbReference type="GeneTree" id="ENSGT00910000144260"/>
    <dbReference type="OMA" id="QSRHNME"/>
    <dbReference type="OrthoDB" id="64353at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16293">
        <property type="entry name" value="CPSF2-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000008">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027075">
        <property type="entry name" value="CPSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025069">
        <property type="entry name" value="Cpsf2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035639">
        <property type="entry name" value="CPSF2_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922:SF1">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13299">
        <property type="entry name" value="CPSF100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Beta-Casp" evidence="10">
        <location>
            <begin position="243"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="787" mass="89206" checksum="6FA0E95C2ED0DB0E" modified="2018-12-05" version="1">MTSIIKLTAVSGVQEESALCYLLQVDEFRFLLDCGWDESFSMDIIDAMKRYVHQVDAVLLSHPDPLHLGALPYAVGKLGLNCTIYATIPVYKMGQMFMYDLYQSRHNTEDFTLFTLDDVDSAFDKIQQLKYSQIVNLKGKGHGLSITPLPAGHMIGGTIWKIVKDGEEEIIYAVDFNHKREIHLNGCSLETISRPSLLITDSFNATYVQPRRKQRDEQLLTNIMETLRGDGNVLIAVDTAGRVLELAQLLDQIWRTKDAGLGVYSLALLNNVSYNVVEFSKSQVEWMSDKLMRCFEDKRNNPFQFRHLSLCHSLADLARVPSPKVVLCSQPDLESGFSRELFIQWCQDSKNSIILTYRTTPGTLARYLIDNPGEKMIDLEIKKRIKLESRELEEYLEKEKMKKEAAKKLEQAKEVDVDSSDESDMEDDLEQPAVMKTKHHDLMMKGEGGRKGSFFKQAKKSYPMFPAHEERIKWDEYGEIIRPEEFLVPELQATEEEKSKLESGLANGDEPMDQDLSDVPTKCTCTTETLEIRARVTYIDYEGRSDGDSIKKIINQMKPRQLVIVHGPPEASQDLAESCKAFSGKDIKVYTPKLQETVDATSETHIYQVRLKDSLVSSLQFCKARDTELAWIDGVLDMRVVKVDTGVLLEEGETKDEIEDGELAMDVTSDLATEPSTVATQRAMKTLFGEDEKEISEESDVIPTLEPLPLTETPGHQAVFINEPRLSDFKQVLLREGIQAEFVGGVLVCNNLVAVRRTEAGRIGLEGCQCEDYYRIRELLYQQYAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4P4</accession>
    <name>A0A3B4C4P4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kelch-like protein 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KLHL21</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL21 and RBX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017576398.1">
        <property type="nucleotide sequence ID" value="XM_017720909.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017576399.1">
        <property type="nucleotide sequence ID" value="XM_017720910.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017576400.1">
        <property type="nucleotide sequence ID" value="XM_017720911.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017576401.1">
        <property type="nucleotide sequence ID" value="XM_017720912.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4P4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006857"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002809.2">
        <property type="protein sequence ID" value="ENSPNAP00000006857.1"/>
        <property type="gene ID" value="ENSPNAG00000012831.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441412"/>
    <dbReference type="CTD" id="9903"/>
    <dbReference type="GeneTree" id="ENSGT00940000158631"/>
    <dbReference type="OMA" id="TWSVVGQ"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18460">
        <property type="entry name" value="BACK_KLHL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18250">
        <property type="entry name" value="BTB_POZ_KLHL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000044">
        <property type="entry name" value="Kelch-like family member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030577">
        <property type="entry name" value="BTB/POZ_KLHL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047069">
        <property type="entry name" value="KLHL21_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF17">
        <property type="entry name" value="KELCH-LIKE PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="614" mass="69859" checksum="7A6B68090355DB76" modified="2018-12-05" version="1">MEKPVLQTQPSMLPFFDTAHAINLLRGIHELRAERKFFDVTLCAEGKEFHCHRTVLAAASTYFRAMFAGTLRESAMDRVVLHEVSAELLGLLVDFCYTGRVTVTHDNVDLLLKTADLFQFPSVKEACCAFLEQRLDVTNCLEIQDFAEAYACRELATSARRFVLKNIVELAKGTDFERLPWKRLLEFVSDDGLCVDKEETAYQIAIRWVKADLQRRLHYWPELLQQVRLPFVRRFYLLAQVESDPLVYLSPACLRLVSEARSFQSCEYDRHDRPCHRMRPRPSTGLAEILVVVGGCDQDCDELVTVDCFNPQTGQWRYLAEFPDHLGGGYSIAALGNDIYVTGGSDGSRLYDCVWRYNSSVNEWTEVSPMLKAREYHSSCVLKGQLYMVASESTERYDHTLDCWEALPPMLHPMDNCSTTACGGRLYAIGSLTAEDTMAIQCYDSDSNRWTLLNCGQLPPWSFAPKSVTLNGLIYFVRDDSAEVDVYNPQKNEWDKINPMNQVHVGGSVAALGGRLYVSGGYDNTFELSDVVEAYDPSTRSWSMAGRLPQPTFWHGSVSIFRQFMPLVPSTFEPIDMPDANDIHLNRHHRHQALHNLNNELNQNHQNREVNAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C605</accession>
    <name>A0A3B4C605_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000005984"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004352.2">
        <property type="protein sequence ID" value="ENSPNAP00000005984.2"/>
        <property type="gene ID" value="ENSPNAG00000002077.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162544"/>
    <dbReference type="OMA" id="IEQRQVC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000045">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1220.10">
        <property type="entry name" value="EGF-type module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF12">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1457"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="621"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="789"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="833"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1410"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1318"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1483"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1318"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1372"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1490"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="358"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="397"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="434"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="454"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="461"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="526"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="545"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="552"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1436"/>
            <end position="1445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005984.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1525" mass="164634" checksum="352D82F4F1D85C51" modified="2024-11-27" version="2">MSARSCASPRDRALIKRVRVGCVCWFAAMRSVCAALCAVLLLHAVTGELDPQQGEPPRCRLGTVPCKDGSDCVLFNHVCDGERDCADGSDEEDCTTECEKGQFQCAHGKMCIEQSQVCDDVAQCQDRSDELDCFKPAEGCGHRCGKLCLSEVLICDGRVDCEDGSDEADCDDESEAEEVEEVHAVGTDPPAVRPPLRCLLGSWACRDGTECIQYTHICDGEEDCRDGSDELECAEECEAGQFQCAHGKMCIEQSQVCDDVAQCQDRSDELDCFKPAEGCGHRCGKLCLSEVLICDGRVDCEDGSDEADCGDESVEAEEVEEVHAVGTDPPAVRPPLRCLLGSWACRDGTECIHYTHICDGEEDCRDGSDELECAEECEAGQFQCAHGKMCIEQSQVCDDVAQCQDRSDEMGCLKLGEDCEHQCGKLCLPGFFICDGYLDCEDGSDEANCGGRTCGNTEFQCASGQCVPISGRCDGIPDCRDHSDERNCSDWTECHEASCAESPVQCGQFQWPCASKTQCVPQSWRCDGTKDCADGSDEAACDSSCPPHQFQCRSSECLDPSVLCNGETDCMDGSDEGGACSTDACTDQSQCAHDCYSTPTGTSCWCRVGYRPVGGAVECVDVDECVETPAVCAHSCINSVGSFQCSCNHGYLLEPDGHTCTATGESYLLASVESELLLLDLQTSTVDVLLSSETLPALSLDYDMQKRSVYWADVEGVKWITLDKKGRGTLLKGVRAGSVAVDWVGRSLYWMDRVDGQINAVGLEGSEPVVIMYKDVEELQALALLPQKGLMFWSETGDEAQIGRAGMDGSDRQVLVQDSVRWPVGLAVDSLKERLYWTDEQLRCIGSATLDGDDVKLLQMMETPSPFSLSVFGDMVYWSDIHKGTIQRAHKVTGKQPQVLLNRPGLPLGLKVFHPLLQPSVANPCESMNCSHLCVLAPGLEGGVCKCPSELVLDEGGLVCHEPKDRTFLLLLSPTAVTQVTLQSRDAGVGLQDWPEHRRIDLPGVQELKKLDLVIQDKALYVWDAGRTSVGLFRVKDSGASQRGTLFSLLNNMLTAMAVDWVTLNLYWSSSSQSGLWVTSAKGTETTLILQDEVRNASSITLQPVAGRMCFTNRVQLGRVQLECSYMDGQKRNVVWVDAVQPVSLSLSNEENRLYWADTSLGVVASVGVDGTAYKEIRTEEGLVTFTLANKVLVWITRRGSTKCWFSNDQQGAMLWFEVKAEIVDVKAFSKPSQTGSNSCSISNGGCAQLCLPHPGGRTCLCGSAFLSINEMGCAPDPRCPSGTQPCVSGEECVPQERFCDGRPDCADRSDEICVQDEAKRIDDPKTNRFRPGSGSLPAGPRSFNTTVLVKNPGSDVDLSHGPKFPPPATPRSRDNVLVKSQDSKTRPASVRPSPTAKGSGSGVKVDSVDAAACGVRLCNGKGECVLQDGATVCQCMAGYSGPHCEEPLGSVVQEPVVYAAGGLCVAVIVLGVTVGIMQKRKAANQRQAGARETRMQDLEKSESVPAPPNSKESEQTEDGASAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDV2</accession>
    <name>A0A3B4CDV2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SDF2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017554113.1">
        <property type="nucleotide sequence ID" value="XM_017698624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CDV2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009703"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016130.2">
        <property type="protein sequence ID" value="ENSPNAP00000009703.1"/>
        <property type="gene ID" value="ENSPNAG00000015181.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427987"/>
    <dbReference type="CTD" id="6388"/>
    <dbReference type="GeneTree" id="ENSGT00940000158885"/>
    <dbReference type="OMA" id="NYWRAME"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23293">
        <property type="entry name" value="beta-trefoil_MIR_SDF2_meta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000023">
        <property type="entry name" value="Stromal cell-derived factor 2-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF3">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017372474" description="MIR domain-containing protein" evidence="4">
        <location>
            <begin position="29"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="5">
        <location>
            <begin position="31"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="5">
        <location>
            <begin position="93"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="5">
        <location>
            <begin position="149"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="224" mass="24544" checksum="16CA9A45B678BC7D" modified="2018-12-05" version="1">MAAANCILSLASLCLSVLLSCMWNFSLGSEISFVTCGSVVKLLNVKHNVRLHSHDVRYGSGSGQQSVTGVTTVEDSNSYWSVRGTIGTACHRGTPVKCGQNIRLTHVNTGRNLHSHYFASPLSSNQEVSAFGEDGEGDHLDEWTVLCGGSLWQRDESVRFRHTATEALLSVTGEQYGRPIHGQREVHAMTSSSQHSYWKAMEGIFMKPSETGPRDFSSPPHTEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGH7</accession>
    <name>A0A3B4CGH7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-L-isoaspartate O-methyltransferase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PCMTD1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Substrate recognition component of an ECS (Elongin BC-CUL5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Specifically binds to the methyltransferase cofactor S-adenosylmethionine (AdoMet) via the N-terminal AdoMet binding motif, but does not display methyltransferase activity. May provide an alternate maintenance pathway for modified proteins by acting as a damage-specific E3 ubiquitin ligase adaptor protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578780.1">
        <property type="nucleotide sequence ID" value="XM_017723291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGH7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010568"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017252.2">
        <property type="protein sequence ID" value="ENSPNAP00000010568.1"/>
        <property type="gene ID" value="ENSPNAG00000016148.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442960"/>
    <dbReference type="CTD" id="115294"/>
    <dbReference type="GeneTree" id="ENSGT00950000183032"/>
    <dbReference type="OMA" id="QSTWDSR"/>
    <dbReference type="OrthoDB" id="10257972at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000015">
        <property type="entry name" value="Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF4">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="359" mass="41004" checksum="C1ED82FAF7B3C94C" modified="2018-12-05" version="1">MGGAVSAGEDNDDLIDNLKEAQYIRTDKVEQAFRAIDRGDYYLEGYRDNAYKDLAWKHGNIHLSAPCIYSEVMEALNLQQGLSFLNLGSGTGYLSTMVGLIIGPFGVNHGVELHKDVVDYAKDRLEEFIRNSDSFDKFEFCEPSFVVGNCLEISSDSHQYDRIYCGAGVQKDHENYMKVMLKVGGILVMPIEDQLTQITRTGQSSWESKNILAVSFAPLVQQSRNDGSKPDAVVLPPFSVRTLQDIARIYIRRTVRNLVNEEALSKGPVQRVPQKRKRRRCRPPRINTYVFVGNQLIPEPMESEEDERIEKESKEEEQDKDTVEPLKPEEPQVNLLRDKILNLPLPEPLKVYLLYYREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CKC6</accession>
    <name>A0A3B4CKC6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ectonucleotide pyrophosphatase/phosphodiesterase 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ENPP7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CKC6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012467"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019752.2">
        <property type="protein sequence ID" value="ENSPNAP00000012467.2"/>
        <property type="gene ID" value="ENSPNAG00000018283.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159339"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF63">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043433662" description="Ectonucleotide pyrophosphatase/phosphodiesterase 7" evidence="1">
        <location>
            <begin position="18"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012467.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="49691" checksum="23595028342E4C06" modified="2024-11-27" version="2">MLWVVCVFLCVCARSLAVPVAGPRISSRSKVLLVSFDGFRWDYDRDVDTPHLDAMARDGVKAPYVTPAFITVTSPTHFTILTGKYIENHGVIHNMWFNTTTSEKKPYYQTQFVNEWWDNGSVPIWITAQRQGLRAGSLHFPGTASTYQGESAEVSEVEPWFYNYSNETAWRENVDKVMGSWFREKDLDFVSMYFGEPDSTGHKYGPDSPERIEMVKQVDRTVGYLRNSAASNGLTDHLNIIITADHGMTTVYRNGLVKEITLSKIPGFSFRDLVFHLVDYGPTGMLLPKEGMLEKVYNALKGAHPHLHVYKKEDMPSRLHFANNARILPIILWSDPGYVINGYFPVQFNKGEHGFDNENLDMKPFFRAVGPAFHKNLVVEPFETVNIYPLMCHLLGITPEPNDGHLGATQAMLISSEPPEVSHERLCITVILPLLWGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CLA4</accession>
    <name>A0A3B4CLA4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450 1A</fullName>
            <ecNumber evidence="14">1.14.14.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CYP1A2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17149"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:142491"/>
            <dbReference type="EC" id="1.14.14.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Microsome membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CLA4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012138"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036403.2">
        <property type="protein sequence ID" value="ENSPNAP00000012138.2"/>
        <property type="gene ID" value="ENSPNAG00000017872.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183037"/>
    <dbReference type="OMA" id="AKWEIFL"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20676">
        <property type="entry name" value="CYP1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000002">
        <property type="entry name" value="Cytochrome P450 1A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008066">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF21">
        <property type="entry name" value="CYTOCHROME P450 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01683">
        <property type="entry name" value="EP450ICYP1A"/>
    </dbReference>
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    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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            <position position="490"/>
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    <sequence length="551" mass="62350" checksum="89002C7FEE2F192C" modified="2024-11-27" version="2">MQICSSFIDVSPSTPSFSLKTLQKTENMPVAVLPFFGPITVSESLVAMVTLCTVYLLLRLTRVRIPEGLLPLPGPQSLPVIGNVLEVGQNPHLSLTAMSKRYGTVFQIRIGTRPVVVLSGSETIRQALIKQGDEFSGRPDLYSFQYINDGKSLAFSTDLAGVWRTRRKLALNALRTFATVESKSPEYACALEEHVSKEGLYLVDRLCKLTEASGSFDPFRQIVVSVANVICGMCFGQRYSHDHQELVSLINLSEEFNKVAGSGNPADFIPILRFLPSKTMKMFLDINRRFNAFVQRIVRQHYDTYDKNNIRDITDSLIDHCEDRKLDENSNLQVSDEKIVGIVNDLFGAGFDTISTALSWSVVYLVAYPEIQERLHQELRDKIGTDHIPQLSEKNSLPYLESFILEIFRHTSFLPFTIPHCTTKDTSLHGYFIPKDTCVFINQWQINHDPEQWKDPFLFNPDRFLCADGSELNKVEAEKVMVFGLGKRRCIGEVIGRSEVFLFLAILIQRLKFSSLPGEMPDMTPEYGLTMKHKRCMLRTAPRPGFEMPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMB1</accession>
    <name>A0A3B4CMB1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017714592.2"/>
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        <property type="nucleotide sequence ID" value="XM_017714593.2"/>
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    <dbReference type="Ensembl" id="ENSPNAT00000019731.2">
        <property type="protein sequence ID" value="ENSPNAP00000012488.1"/>
        <property type="gene ID" value="ENSPNAG00000018202.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00530000064557"/>
    <dbReference type="OMA" id="KIFHCPD"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger 45-like"/>
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        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger protein 770"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="Zinc_finger"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
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            <begin position="515"/>
            <end position="537"/>
        </location>
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        </source>
    </evidence>
    <sequence length="1503" mass="161363" checksum="10DE38A3FA46C82C" modified="2018-12-05" version="1">MFQFGKYNVDIIEMLSGHQAHQLKGLGIERQLQHQQQVQLHQHQLQQQQQQQVETSGAILSGLGLGPLQGSRSSAFSNSTSIFTKMSAPPPPLQQQSLSSSSQGSRKSSKMSGSSSGGSHVSGYPQFLRSFHPTEAALAQEHLHPGVGRFDQFAGSGGSGGSSGGIGGLVSSIPPPPPPLHPGLSVPQASPGPSSSSPSPSSSVSSSNNPPSSSTVTSLGHQLAGAQSDARSLHQQFSCMLAANQYFLSGVPANSSLEQFLVQQGSHNHLGLGLGQTGGESSSSLAPPPALHSSHSHSHSTTLPQPQQPPPQQQQLPPHTLSHPHPHPHHPLHSSSQPSSLGSFDFQGIPVLSSNQLASLMQQEAGLPLPLPLHLSLSKDEGKGDSASGGSGSGGGRRKKAMAGYLPQRKADNSNNNSGSSNSHNSTNPNPSSSTSGSLNQDNASSLGGGGGIIGIGGEPPSHMTPSSSSSGVSSSSSSAPSSTSASVLVTNSSKADNHGSMHPPSSQPEAEPLYHCGECGKSFTHLSSLRRHLRSHEVTTAGTAGTRSNIVNPVLHPADPCIPHSTQDVPATSSCPSPDKTFHCSDCGKGFKKKGHLLQHGVIHSGARPYACSTCSRAFNRRESLTRHEKIHEEKPFRCPACGRCFRESTSLLNHAASGTCGKSGRGPRPKNDSSNPVGGKSNKTGASSDGMVTSAVGSYRSDSYSDSYKEQRSQGSLYSGTSSCGGGLAGPSLRKAPLAPTLHPHPQNQSQSQQHHHQPPHLPLSSLLDDSEDDVTSSVNNAISAITAAAANCMGGDLNTSGARGDDRRDIIGGLLGGLGLGPLASPGGPSSTSGMDKSFRGGPNQDALTSSQQPNQQTPSGKPKRPRKPRKKKEPGAPGSEPPKRRQPSQRTVGVLGEVRPFLCSICGRGFARRETLRRHDRIHTGEKPHHCTVCGKYFREAFHLSKHHTVHSGEKNYKCLLCGKDFGYAQSLKRHGKLHQKGELEEVPATPGGDNLNNYPSASGSMIQGGQGSSSSFYSYSQDVKPQGTNPQPAPRLYTCAICWKSFRHHFHLTAHHQTVHEGGGDKLFSCEVCGKAFAYSNSLTRHRLSQHGLTRNGQGTSQGSGNGAGEDSNVAGSISESEAATNALLQLAPSSGSQGEQQSHPGVLHSHQQPPPQPQAGYSPLFYVPEASTSHPSSSNATPYSQPLPSSSSISSLNHQHSVVKGEPMYQTGQRHVLNPNLPVHPLVLPPPDHQHHSHPQQHPEDIQPHHHHHHHHHHHSFHSEDELRRRKKKKKRRIGREKIGYEWSRTAKMDGGQGVLSAGQRKRNERRILQKRRKRAIRRRQRKFRRKMAMLLRIRRGGSTRVVYELGSTEGLELYRLFSWQVPLKRFPCPFCFLTTFSRRAALVVHIAARHCPKVSYRVDRLRCLVCGKQSRRFLSALIHRSSHLSNRAFSCRRCPSRFWNAALLTRHKRTCRGKLAELRQGRTLCFKLTAGNFFKRVEGKGNISTLQIQYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNC8</accession>
    <name>A0A3B4CNC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017543870.1">
        <property type="nucleotide sequence ID" value="XM_017688381.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNC8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013542"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021177.2">
        <property type="protein sequence ID" value="ENSPNAP00000013542.1"/>
        <property type="gene ID" value="ENSPNAG00000019397.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415359"/>
    <dbReference type="GeneTree" id="ENSGT00940000166490"/>
    <dbReference type="OMA" id="YLMIYRH"/>
    <dbReference type="OrthoDB" id="253091at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF16">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="30"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="98"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="6">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="185" mass="21266" checksum="BCC9281C02605572" modified="2018-12-05" version="1">MTAQKNQRKEYLCVKDLEKVLDSCTLELNQIDEVWPNLYIGNVRIAQNRAALQRLGITHILNAAHSKRGSIGDQNFYGTSFVYCGIPAEDSSYFDLDIYFRPAAEFIHKGLKTPDGKVLVHCIMGMSRSSTLVLAYLMLYHHIPLRSAIQRLIRKRAIYPNRNFLALLLDLDLQLKRKRKTCILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQC5</accession>
    <accession>A0A3B4CNF9</accession>
    <name>A0A3B4CQC5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Nephronectin a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nephronectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556894.1">
        <property type="nucleotide sequence ID" value="XM_017701405.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQC5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013565"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021210.2">
        <property type="protein sequence ID" value="ENSPNAP00000013572.2"/>
        <property type="gene ID" value="ENSPNAG00000019459.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000021229.2">
        <property type="protein sequence ID" value="ENSPNAP00000031863.1"/>
        <property type="gene ID" value="ENSPNAG00000019459.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000036990.2">
        <property type="protein sequence ID" value="ENSPNAP00000013565.1"/>
        <property type="gene ID" value="ENSPNAG00000019459.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429572"/>
    <dbReference type="CTD" id="563273"/>
    <dbReference type="GeneTree" id="ENSGT00930000150973"/>
    <dbReference type="OrthoDB" id="10060424at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003171">
        <property type="term" value="P:atrioventricular valve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060347">
        <property type="term" value="P:heart trabecula formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000187">
        <property type="entry name" value="nephronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000476">
        <property type="entry name" value="nephronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000184">
        <property type="entry name" value="nephronectin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000268">
        <property type="entry name" value="nephronectin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF19">
        <property type="entry name" value="NEPHRONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044589689" description="Nephronectin a" evidence="15">
        <location>
            <begin position="20"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="141"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="221"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="266"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="17">
        <location>
            <begin position="490"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="308"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="404"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="71129" checksum="5B2F95E139917871" modified="2018-12-05" version="1">MEMLLKLVLLWTCWLGVGADFDGRWPRQMASSNGLCRYGARIDCCWGWTRRSWGHCQPLYILTNRVIGIKCQAQALCQHGCKHGDCVGPNKCKCHPGFTGKTCNQEEEPDYINPPMPLWDSYPHVFIPTMDQQPVGVPPEDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLLPDGTCGNARTCSMANCQYGCEVMKGEVRCQCPSPGLQLAPDGRTCVDVDECATGLVVCPRFRKCINTFGSYICKCHDGFDLQYINGKYQCIDVDECSTGQHQCGPYSSCYNTPGSFKCKCKDGFRGGLGHDCKPIPKVIIEPPRPGKTTPSNNNRIPDADQRRTTTTARPPVTPKRTPATTPSTTTTTVKAPSPPKTAPPPPPPPKKPTIPTRKPPVPTRRPPVITQKPYVPPRRPATTKAPPPTPRLPVIPTRRPPAPPVTQLDNNINKDITEKQRGDVHIPRNHGQNNVLDIDYDIELGNTEEEVKDDPEAGYLSCSFDHGLCGWIQVREGDVHWETVPDPSGGKYLTISEVLGKRSGRGAQLVLPLTMPWNEGNLCLAFRHKMAGHHVGMLQVFVQKGRQHSPAVWGRTGGSGWRSTQITLWGNGLESVILKGERRRGRTGELALDDISLRRGACQEEHNLRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQZ9</accession>
    <name>A0A3B4CQZ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Citrate synthase-lysine N-methyltransferase CSKMT, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Methyltransferase-like protein 12, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CSKMT</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Protein-lysine methyltransferase that selectively trimethylates citrate synthase (CS) in mitochondria. Seems to conduct trimethylation in a highly distributive manner rather than in a processive manner, and thus introduces a single methyl group per binding event.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[citrate synthase] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[citrate synthase] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55544"/>
            <dbReference type="Rhea" id="RHEA-COMP:14212"/>
            <dbReference type="Rhea" id="RHEA-COMP:14213"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6),N(6)-dimethyl-L-lysyl-[citrate synthase] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[citrate synthase] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55552"/>
            <dbReference type="Rhea" id="RHEA-COMP:14214"/>
            <dbReference type="Rhea" id="RHEA-COMP:14215"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-methyl-L-lysyl-[citrate synthase] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl-[citrate synthase] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55548"/>
            <dbReference type="Rhea" id="RHEA-COMP:14213"/>
            <dbReference type="Rhea" id="RHEA-COMP:14214"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565378.1">
        <property type="nucleotide sequence ID" value="XM_017709889.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQZ9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013783"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021480.2">
        <property type="protein sequence ID" value="ENSPNAP00000013783.1"/>
        <property type="gene ID" value="ENSPNAG00000003600.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434625"/>
    <dbReference type="CTD" id="751071"/>
    <dbReference type="GeneTree" id="ENSGT00510000049875"/>
    <dbReference type="OMA" id="LMQEHIQ"/>
    <dbReference type="OrthoDB" id="411785at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000200">
        <property type="entry name" value="Citrate synthase lysine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF83">
        <property type="entry name" value="CITRATE SYNTHASE-LYSINE N-METHYLTRANSFERASE CSKMT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Methyltransferase" evidence="14">
        <location>
            <begin position="84"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="251" mass="28166" checksum="2F8BE98EB1A2C8E5" modified="2018-12-05" version="1">MLRIIRPCLSLRSFGKEAGRRWHRASSTNDLIENMDKKATWDRFYTENSKGTFKNFEWFFGFHAIKDCILPLLQTASTTDAPLYVLDIGCGTSALGPCIYRYSECAVKVTCADISPVAVQLMQEHAENMPVQPCNPSSTLVFQELDCTELERHFGPKSLALIVDKGTTDALVRSKKGQEKAGQIIRQCLKLLRPSGNLLQFSDEDPDARLLWLEKESRGSEVAAEVGVHEIGEIRGVTYFCYQISPCIPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSQ6</accession>
    <name>A0A3B4CSQ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SOX17</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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                <person name="Meyer A."/>
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                <person name="Pippel M."/>
                <person name="Winkler S."/>
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                <person name="Wood J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the TMEM121 family.</text>
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        <location>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="364" mass="39717" checksum="FF1441AE71EB5C2E" modified="2018-12-05" version="1">MRERGTHPASSTSRQGMLPSPQVCVSTLVTVSTMAVVDLYLLEQSMLGPRGSGRPAVWPCVAVALGDLCFLLALRFVSAGVVQEARSPRRGFANALWFLFLSLLQLKLFFVCQNYRQERRPPDPLARKTLTLLLSVCLPSLFLILTGADHMTPLRRKQEVRSRLLWVVVDLLDVLDLQAGLWEAQSSVGVPLWVDGIVFFYCYVLLLLLPCVSLTELGAAALPGLRGPRREAVYPWLSLITINIFTLALRGVGMLWYRDPRVSTVFLGKNLLALAVKLSSAWERRRQGGGRGQGAGADVGAESQSLGAAMDQGEEQGQGHTSPVPPSSRYHSLSRAHGHGHSVSRGSLDPAETSLGPAYISHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVZ7</accession>
    <name>A0A3B4CVZ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVZ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014941"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022993.2">
        <property type="protein sequence ID" value="ENSPNAP00000014941.2"/>
        <property type="gene ID" value="ENSPNAG00000020906.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144296"/>
    <dbReference type="OMA" id="KDQAPPR"/>
    <dbReference type="OrthoDB" id="6157464at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22076">
        <property type="entry name" value="WH2_WAS_WASL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053099">
        <property type="entry name" value="WAS/WASL-interacting_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48226">
        <property type="entry name" value="OS06G0326200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48226:SF1">
        <property type="entry name" value="WAS_WASL-INTERACTING PROTEIN FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014941.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="529" mass="52786" checksum="3C831EA6CF129174" modified="2024-11-27" version="2">MPGPPPPPPPPPPTFASANTEKPVLKKSEQQGRNALLTDICKGTRLKKAVTNDRSAPVFEKPKGGGGGGGGGGGGGGFGGGAPGGLGGLFQGGMPRLKSPGSRDTSARPPMPPSGGRSPTPGGGAPPRLSGTLPGRSPMNKGSIPPVPASMPSPQRNSHPTGPPSLPGGRPPNSSHSTQSTTPGRRPSLPPTPAGGPPGRQTNLPPPPVPSSGRPPLPTVPSLSSDDFPPPPPSMGGSRGSFPPPPVHSSGRPPLPTVPSASSDDFPPPPPSIGGNRASFPPPPVPSSGRPPLPTVPAASSDDFPPPPPPVGGHRASFSRDGPPPPLPPTGNKPPVPSAPRPTGNTPPPPPSGRGDLPPLPPAPREENRTPQRPPSSSSLPPVPRPGGSGPPPPPPSARPPQQGRSPSVRSGPLPPPPPSGGSRGGTPPAPPPNRPGGGGGGGRPPPPPDRPGTGGFPPPPPSTIGNGYQSSPQHSQDDWEMRFSFHSLSELPPPEPYVSCPKTYPSKAGSLQKERGAPPLPPLPPSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CXB4</accession>
    <name>A0A3B4CXB4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CLTA</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017579610.1">
        <property type="nucleotide sequence ID" value="XM_017724121.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CXB4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015421"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023623.2">
        <property type="protein sequence ID" value="ENSPNAP00000015421.1"/>
        <property type="gene ID" value="ENSPNAG00000021477.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443445"/>
    <dbReference type="CTD" id="1211"/>
    <dbReference type="GeneTree" id="ENSGT00940000157347"/>
    <dbReference type="OrthoDB" id="5512at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099631">
        <property type="term" value="C:postsynaptic endocytic zone cytoplasmic component"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639:SF1">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00224">
        <property type="entry name" value="CLATHRIN_LIGHT_CHN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00581">
        <property type="entry name" value="CLATHRIN_LIGHT_CHN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="231" mass="25955" checksum="79757201E8841620" modified="2018-12-05" version="1">MDDFDMLGNAGNGVGAEEDPAAAFLAQQESEIAGIEQDEGFSILDGGEVPSHLAADQHNGGALNGDLHESNGPSDAYAAISSADRLQAEPESLRKWREEQRERLEDLDANSRKQEVEWKEKAKVELEEWHARQDEQLAKTKVNNRVLDEDFYKQPFAHLIGYVTHINHPCYRLDQAAEEAMVTELNESNPGTEWERVARLCDFNPKSNKQAKDVSRMRSVLISLKQSPLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYP9</accession>
    <name>A0A3B4CYP9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">UBXN7</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017578669.1">
        <property type="nucleotide sequence ID" value="XM_017723180.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYP9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016480"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024988.2">
        <property type="protein sequence ID" value="ENSPNAP00000016480.1"/>
        <property type="gene ID" value="ENSPNAG00000022692.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442896"/>
    <dbReference type="CTD" id="26043"/>
    <dbReference type="GeneTree" id="ENSGT00390000018687"/>
    <dbReference type="OrthoDB" id="270602at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14345">
        <property type="entry name" value="UBA_UBXD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01773">
        <property type="entry name" value="UBX_UBXN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000079">
        <property type="entry name" value="UBX domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054109">
        <property type="entry name" value="UBA_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017346">
        <property type="entry name" value="UBX_7/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22566">
        <property type="entry name" value="UBA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037991">
        <property type="entry name" value="UCP037991_UBX7/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="438"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="303"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="385"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="519" mass="57592" checksum="7FE7C1ECAED89EF2" modified="2018-12-05" version="1">MEALGDAAAPGLKGLIQQFTAITGATESVGRHMLEACNNNLEMAVTMFLDGGGITEEPSTSSSSAGASGSRHPHSEDDVRAPIPQKQEILVEPEPLFGVPKRRRPARSIFDGFRDFQTETIRQEQELRNGGAMDKKLSTLADLFRPPIELMHKGSFETAKDCGQLENKWLMINIQNVQDFACQCLNRDVWSNKAVKTIIREHFIFWQVYHDSEEGQRYIQFYKLNKFPYISILDPRTGQKMVEWNQLDVSSFLDQVTGFLTEHGQLDGLSTQPPAKRARSESLIDASEDSQLEAAIRASLQETHYESTENKPESRSDDESDAEAFSDSEGLISVDGSDNETGGGGGGGGGGGGGGEESLDGRVSSEQAPPTNRQDSPAHHRKSPHKELNHRKEESKKNHLEPPGPSHRPPESQQQRQNQNSTPPHSSQPTAGSSEAEDNGPKARLMLRYPDGRREQIALSANDKLMALVRHVQSKGYPNERFELVTNFPRRKLAHLDYEITLQEAGLCPQETVFVQERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0P2</accession>
    <name>A0A3B4D0P2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0P2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016626"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025178.2">
        <property type="protein sequence ID" value="ENSPNAP00000016626.1"/>
        <property type="gene ID" value="ENSPNAG00000022788.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000288895"/>
    <dbReference type="OMA" id="IRNAITC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="89" mass="10448" checksum="E58A1940A465659E" modified="2018-12-05" version="1">MNMTKKRRRPMLNRAGRDIIKANRRVRIPLAPLIRRSIRPIRASRMTRNNVGETKYSMTPETQVPVEKGENIRNAITCYSSKCKLIYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6Y6</accession>
    <name>A0A3B4D6Y6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dickkopf N-terminal cysteine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dickkopf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6Y6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018886"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039192.2">
        <property type="protein sequence ID" value="ENSPNAP00000018886.2"/>
        <property type="gene ID" value="ENSPNAG00000006000.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000221"/>
    <dbReference type="OMA" id="GPSMCCA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039706">
        <property type="term" value="F:co-receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.80.10:FF:000010">
        <property type="entry name" value="AmphiDkk3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006796">
        <property type="entry name" value="Dickkopf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039863">
        <property type="entry name" value="DKK1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113:SF31">
        <property type="entry name" value="DICKKOPF N-TERMINAL CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113">
        <property type="entry name" value="DICKKOPF3-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04706">
        <property type="entry name" value="Dickkopf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Dickkopf N-terminal cysteine-rich" evidence="8">
        <location>
            <begin position="3"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018886.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="140" mass="15443" checksum="2BA3D6903DD2053F" modified="2024-11-27" version="2">TSSCGKGFSCDRHFGLCVPLRQEGKYCRRDAQCVRGLSCMFGKCIRSIPEGQEGARCKVDKDCGSSMCCARHHGEMVCKRRLALGESCFVPDGGLAFSINQICPCDEGLLCRGNGRPQKRETEFVYQSDSSSWTCQVPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7T1</accession>
    <name>A0A3B4D7T1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="21">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">USP8</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 21">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7T1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019176"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028900.2">
        <property type="protein sequence ID" value="ENSPNAP00000019176.1"/>
        <property type="gene ID" value="ENSPNAG00000006058.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157542"/>
    <dbReference type="OMA" id="YKYDDHE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000025">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000011">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000017">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048498">
        <property type="entry name" value="WW_USP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF27">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08969">
        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20625">
        <property type="entry name" value="WW_USP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="13 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 21" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="14 21" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12 21" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="23">
        <location>
            <begin position="663"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="318"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="555"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="330"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="421"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="505"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="562"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00063318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="995" mass="112325" checksum="CD3992514E8714E9" modified="2018-12-05" version="1">MPAVSTGLKELYLSTSLGDLNKKAEIKPDKVSTRHYVQSACKIFKAAEECRLDRDEEKAYVLYMKYLTVYDLIKKRPDFKQQQDFYLSILGPTSFKKAIEEAEKLSESLKLRLESCSRSHICLPLSVCVSIAAPSGGITARQLYSMMLDSSMSVLVMDARKLADFEESHMMVQDQKCITVPEEAVSPGMTVNQIELKLPAASLPLWKQRGSVDYIILLDWFSSVSDLKLGTTLLSLKEALFKWDSVTILRSEPMVLEGGYENWLLYYPMFTTNARVKPPRQDTLNTLPQLNFSYPCLEEPQVESVTVKDGVGEAASVQVNGKVSEEVSHTEPTGPTGPTTHTPTHTPTHTPTHTPSVQPAASAVTPSAAKQPAATAKPIPQIDRTKKPAVKVSDGGKASEEGGAKDAPVSTATNGPVIPDRSAKPFLDEERREREAAEKRQREERERRRGEEEQKQEQRKLERQKAEDEDEEGEERGRRGAKEALDAQIKSMSLDSPLPNSTVSEIKREPLTRARSEEMGRTVPGLPEGWMKFLDIVTGTYRYYHSPTNRVHLYPPEVSVPQTPPATPPTPKQKPPRPSASEKEKEKEKEREREREREQSKLKRSYSSPDIKGPRKPTPTPTINRDTKPLTATSYAKVEVARPSAAKIRNMNPVFGGLGPCLTGLRNLGNTCYMNSILQCLCNSPLMADYFNRNLYQNDINRANILGHKGEVAEEFGVIMKAVWSGQFKFISPRDFKNTISKINEQFSGYEQQDSQELLLFLMDGLHEDLNKADNRKRCKEEVIDHLDDVSAAELAWSKHKLLNESIIVAMFQGQFKSSLQCLSCHRKSRTFETFMYLSLPLASSSKCSLQDCLKLFSKEEKLTDNNKVYCPHCKGHREFTKKLEIWKVPPILLVHLKRFWYEGRWKQKLQTSVDFPLENLDLSQYVIGPKTSLKRYNLYAVSNHYGGLDGGHYTAYCKNAASQHWFKFDDHEVSEISTSSVKSSAAYIFFYSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBE6</accession>
    <name>A0A3B4DBE6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 20">Phospholipase A2</fullName>
            <ecNumber evidence="2 20">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40427"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73003"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40428"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73008"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40816"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40919"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72841"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:40920"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38780"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N,1-dihexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = N,1-dihexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:85334"/>
            <dbReference type="ChEBI" id="CHEBI:85335"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:56425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:78097"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:45425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="2 20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBE6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030716.2">
        <property type="protein sequence ID" value="ENSPNAP00000020269.2"/>
        <property type="gene ID" value="ENSPNAG00000027495.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154885"/>
    <dbReference type="OMA" id="RSEWNPE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000011">
        <property type="entry name" value="Phospholipase A(2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF94">
        <property type="entry name" value="PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17 20" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 20" id="KW-0964">Secreted</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043111498" description="Phospholipase A2" evidence="20">
        <location>
            <begin position="22"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="21">
        <location>
            <begin position="27"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="53"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="70"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="77"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020269.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="180" mass="19457" checksum="CABB3B1478060A87" modified="2024-11-27" version="2">RATITSLVCLLVCLLVGWLYADWSERSLLQFRAMIKCVLPNSSPLEDFADYGCYCGFGGQGTPVDQLDQCCFVHDHCYSMASNLSSCDSLLDSPYVNIYDFRCDSATNTVTCMSDNKECDMFICECDRVASACFGVAPYNVSNNHLSSSLCKSASGGSSSSALTTVSFALILVLKLTSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDJ1</accession>
    <name>A0A3B4DDJ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559432.1">
        <property type="nucleotide sequence ID" value="XM_017703943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDJ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021131"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032156.2">
        <property type="protein sequence ID" value="ENSPNAP00000021131.1"/>
        <property type="gene ID" value="ENSPNAG00000005104.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431054"/>
    <dbReference type="CTD" id="799447"/>
    <dbReference type="GeneTree" id="ENSGT00940000158728"/>
    <dbReference type="OMA" id="ESPRSHQ"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002302">
        <property type="term" value="P:CD8-positive, alpha-beta T cell differentiation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001714">
        <property type="term" value="P:endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000004">
        <property type="entry name" value="T-box, brain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032385">
        <property type="entry name" value="T-box_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF13">
        <property type="entry name" value="EOMESODERMIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16176">
        <property type="entry name" value="T-box_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="7">
        <location>
            <begin position="130"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="550" mass="60173" checksum="4A3E6083277C901B" modified="2018-12-05" version="1">MMAGEADSAALLAAQRKSSPLRCEGELGGGAPYSADGLSAGHFLPAPCAPPPDGLRSFLPYSGASGVVSGGAEGSEGSHYVAASVHYSPALPALSGPAPAFPPSVRLQLSGMYQQYEGPGTPRGRAQVYLCNRALWLKFHRHQTEMIITKQGRRMFPFLSFSIAGLNLASHYNVYVEIVLADPNHWRFQGGKWVTCGKADNNMQGNKVYVHPESPNTGAHWMRQEISFGKLKLTNNKGATTSNGQMIVLQSLHKYQPRLHIVEVLPDGADDVRREPPSQIFVFPESQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTAPEGERLTPSPTDSPRAHQIVPTGRYAMQPFLQEHHLGNPPQTRFYSSERAVPQTARMEDCGAPHRWLVTQQGGCSKLELTAYEGDYSSGLLPAYTLKSLQGAHSLGYYSDSAFTSVAAGWGSKVTYQKKVAPGLPWSPRPNPSEFSDSQLADKDRVQQEELCPAPAWVDTAPFLKPLDTDTGVYSVVCKRRRLSSPESSPAVKCEESRGLDAFSKDTKSYCAFYSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DED8</accession>
    <name>A0A3B4DED8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UBX domain-containing protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">UBX domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">UBXN4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in endoplasmic reticulum-associated protein degradation (ERAD). Acts as a platform to recruit both UBQLN1 and VCP to the ER during ERAD.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Directly interacts with VCP. Interacts with UBQLN1. Forms a complex with VCP and UBQLN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037394904.1">
        <property type="nucleotide sequence ID" value="XM_037539007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DED8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021828"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033493.2">
        <property type="protein sequence ID" value="ENSPNAP00000021828.1"/>
        <property type="gene ID" value="ENSPNAG00000005435.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425946"/>
    <dbReference type="GeneTree" id="ENSGT00940000160205"/>
    <dbReference type="OMA" id="FEPNNTS"/>
    <dbReference type="OrthoDB" id="2445133at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16117">
        <property type="entry name" value="UBX_UBXN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424:SF1">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23187">
        <property type="entry name" value="UBX7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0834">Unfolded protein response</keyword>
    <feature type="domain" description="UBX" evidence="8">
        <location>
            <begin position="326"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="110"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="458"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="206"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="479"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="517"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="527" mass="58269" checksum="80766FF9982D8591" modified="2018-12-05" version="1">MRWFEGSVPAAIAASKQQNSIFVVVITGDDEQSKQMLSSWEDDRMAELTDNCCVAIKVDAKSETCVQFSQIYPVVCIPSSFFIGENGIPLEVIAGGVSAEELTKRINKVKQMHSQQMGSGAQANAERDMQTESSCAAPDAAPSASESHSVNSPAITSENPSTAATSKESLSMPVEEGGASGHATPGEDRSLSSDDASQASQAEEPLDAKVERLTKKLEERREQKKKGEEENEIKKEIERRKLGKEMLDFKRKQEDEKTKRILEERNREKAEERAARERVKQQIAQDRADRAARYAKNKEEAEAARIAALQARQAEQEMKKEATERERSAVARIQFRLPDGSSLTNQFPSETQLQEARQFAAQEVGNRYGHFSLATMFPRREFTAEDLGKTLLELELAPSASIVLLPQSGRPTNTVVQSSGSGGIWALLGTILYPLLAVWRFLSGFIFSSPPPGAAAAAAAASRTQQRSSNSFNSASTGEPKRETVRKRVLEKRPEDFKKDGKIYRLRTQEDSEDDNNTWNGNSTQQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DG91</accession>
    <name>A0A3B4DG91_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DEAF1 transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">DEAF1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017562187.1">
        <property type="nucleotide sequence ID" value="XM_017706698.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DG91"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022520"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034712.2">
        <property type="protein sequence ID" value="ENSPNAP00000022520.1"/>
        <property type="gene ID" value="ENSPNAG00000029908.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432688"/>
    <dbReference type="CTD" id="10522"/>
    <dbReference type="GeneTree" id="ENSGT00940000159701"/>
    <dbReference type="OMA" id="KDHQHSC"/>
    <dbReference type="OrthoDB" id="437457at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000004">
        <property type="entry name" value="Deformed epidermal autoregulatory factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000770">
        <property type="entry name" value="SAND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024119">
        <property type="entry name" value="TF_DEAF-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10237:SF1">
        <property type="entry name" value="DEFORMED EPIDERMAL AUTOREGULATORY FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10237">
        <property type="entry name" value="DEFORMED EPIDERMAL AUTOREGULATORY FACTOR 1 HOMOLOG SUPPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01342">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00258">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50864">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAND" evidence="11">
        <location>
            <begin position="158"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="12">
        <location>
            <begin position="462"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="523" mass="55810" checksum="CC79811F67FFA8DB" modified="2018-12-05" version="1">MDESEPAAKRLGSAEAAEKEGAESETESEAEVPTMTVMGEAGNIDIAESLPNPDDAETAFAEVTTVTVGGVQTADDNVFSTSVAAAASIPEHVLTGRTTLQIGDNLSTQKATLIVVHTDGSIVDATGLKATTAPLTPGPQTPSTPLTPSHEKDVSKYNWDPSVYDNELPVRCRNTSGILYKNRLGSGGKGHCIKHNNNWYTPTEFEGLAGRASSKDWKRSIRYAGRPLQCLIQERILNPHAASCTCAACCDDLTVNKDGSFGGESISMTGPVRLFVPYKRRKKENDRPASPEKKDPQPPKNITLAPGAAFTVSPSGQLTTSGTLTFDRTGTADTTAIISESPTPSDVFSSTTVLTTLPALAVVPQQTVVQTKAPVAGGVVNGLETSEQRTWLYLEETANTLLNTVQQLKTLIAQAKQSSQANQQASSGLDKNCNRKDSFPSQLSLADDPEGKITEIIIKHTCVNCGREASNECAGCRKVHYCSSFCQKKDWKDHQHSCSQPSVGVGLHDEAHIATVEVEKVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHV2</accession>
    <name>A0A3B4DHV2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Histidine ammonia-lyase</fullName>
            <ecNumber evidence="3 10">4.3.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HAL</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>L-histidine = trans-urocanate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21232"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="EC" id="4.3.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PAL/histidase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.3" evidence="3 10"/>
    <dbReference type="RefSeq" id="XP_017562159.1">
        <property type="nucleotide sequence ID" value="XM_017706670.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHV2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023065"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013588.2">
        <property type="protein sequence ID" value="ENSPNAP00000023065.1"/>
        <property type="gene ID" value="ENSPNAG00000007611.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432673"/>
    <dbReference type="CTD" id="3034"/>
    <dbReference type="GeneTree" id="ENSGT00390000009047"/>
    <dbReference type="OMA" id="YSLRCMP"/>
    <dbReference type="OrthoDB" id="10051290at2759"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00549"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004397">
        <property type="term" value="F:histidine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000007">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000003">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000111">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005921">
        <property type="entry name" value="HutH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021922">
        <property type="entry name" value="Par3/HAL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01225">
        <property type="entry name" value="hutH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006871">
        <property type="entry name" value="PRK09367.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12053">
        <property type="entry name" value="Par3_HAL_N_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Par3/HAL N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="630"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="654" mass="72374" checksum="5DEFB315D8D89705" modified="2018-12-05" version="1">MPRFTVHVRDEWLTVPCKDASYSINWLGVEALKRYAKNKPDNGGIPNIKDVRFVARRCQGQGLLDGDDTIEDVLEDNDFVELEIEGDTMSSDFIPSQPGMSYLIRPYREPDEYIFLDGNSLCSTDLVNLGRGRYKIKLTPEAEKKVEQSRELLDTIVKEKKVVYGITTGFGKFARTVIPISKLKELQENLIRSHSAGLGSPLSPERTRMLLALRINVLAKGYSGISLETLHTMIQAFNASCLSFVPEKGTVGASGDLAPLSHLALGLMGEGKMWSPKSGWADAKYVLEAHGLRPISLKPKEGLALINGTQMITSLGAEAVERAESVARQADIIAALTLEVLKGTTKAFDSDIHELRPHLGQKEVALRFRSLLDSDHHPSEIAESHRFCDRVQDAYTMRCCPQVHGIVNDTIAFVRNIINTEINSATDNPMVFAERGETISGGNFHGEYPAKALDYLAIGVHELASISERRIERLCNPSLSELPAFLVNEGGLNSGFMIAHCTAAALVSENKVLCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAACQGIEFLRPLRTTTPLEKVYDLVRSVVKPWIKDRFMSADIEAVHRLLVDQKVWNVAKPYIEKYCTELLPESRPTSPTAFSLEPPPSPRKRVRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJN6</accession>
    <name>A0A3B4DJN6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SEFIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017561203.1">
        <property type="nucleotide sequence ID" value="XM_017705714.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017561204.1">
        <property type="nucleotide sequence ID" value="XM_017705715.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJN6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023331"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012914.2">
        <property type="protein sequence ID" value="ENSPNAP00000023331.1"/>
        <property type="gene ID" value="ENSPNAG00000007864.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432105"/>
    <dbReference type="GeneTree" id="ENSGT00940000168503"/>
    <dbReference type="OMA" id="QCPFATS"/>
    <dbReference type="OrthoDB" id="9949622at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027841">
        <property type="entry name" value="IL-17_rcpt_C/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF12">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15037">
        <property type="entry name" value="IL17_R_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017203382" description="SEFIR domain-containing protein" evidence="13">
        <location>
            <begin position="16"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="SEFIR" evidence="14">
        <location>
            <begin position="460"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="695"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="696"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="720" mass="79402" checksum="5AB7836D01793356" modified="2018-12-05" version="1">MRLFTLTLFFILTAAEKLETIEFNETNALTCGQGLTDCKVNPGDFCVDRDDRVYVSSLAAVALLCHSHKGWLPCLRIQLNITIEGSHEDSGVSGEAVHEGERDESKEEEHGSSLGHFAAVTDSVFVQVCYSIPDFSGSKELTFTPSISTDHPTQVWMSLVVELSQAHFGSKFTIFSSAIHNFSEQVNTLPSIDTVCSQGLDIPLCNGPIVYKEVNHATGMVKLHIDDADRNILNELRACQKMEKEGECYKLDWKENPLMIPLSSAAPCLCFQVWWKTSKGAPRNEFCPFSDTALSDSNVSVSVVEQRKAHDGPVPRNTTALVWNITAPCRLEAEIQLCKKSWSDTGCHEIHDLSKTRLHIHRHHHSKWRQLKQQWHFQGKFAEVERHPSLCVQITVKGMEGNFKLLCPFEVSRTHWSFVFLVCVVLICLTVLGAYAVQGALKGWVFKWLKVDDLKGAVGSSQVVLLYPPDADSGLAELVCRLGSGLSSLGFNVSLDLWSHSELSALGPVPWLHSRLDRVQRHGGKVVLVLTQAAWARAEEWGRRAGAREELKMEKDKEDSGPHVSASPYSDVFSASLSCILADYLQGRAGERFVLAQFEAQPAGPLAAGGPLPELFRGLPLFSLPSQSLGFLTELTNGTQKRQAGRVANRRMRAGVLRAGSRALADALHGLRGGTGYKLVGLSQGFRTEDPWESIPLQQGLSSPTASPELHNKSSTVNWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKC8</accession>
    <name>A0A3B4DKC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubby C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKC8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023384"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012761.2">
        <property type="protein sequence ID" value="ENSPNAP00000023384.2"/>
        <property type="gene ID" value="ENSPNAG00000000542.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158771"/>
    <dbReference type="OMA" id="YAKFIRD"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.90.10:FF:000001">
        <property type="entry name" value="Tubby-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF12">
        <property type="entry name" value="TUBBY-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="5">
        <location>
            <begin position="70"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023384.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="315" mass="35868" checksum="636FC527EAC9373A" modified="2024-11-27" version="2">MFQTGREKEKEKDKDKKFKKSKSPAKAEESDDSDSEVTQKKKRGKGKKGKKEERAPSPVIEFEDLEEFVLQPAQQGVTVKCKVTRDKRGMDRGLYPTYYLHLDNEKKVFLLAGRKRKKSTTSNYLISTDPTDLSRGGENFVGKLRSNLMGTKFTVFDNGLHPDRALPDMSNARQELAAIIYETNVLGMKGPRRMTVIIPGMNKDGDRVPLRPRSENDGLLIRHQNRNMENLIELRNKTPVWNEETASHVLNFNGRVTQASIKNFQIIHNKDQEYIVMQFGRVADDAFTLDYNYPLCAVQAFAIALSSFDGKIACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKG3</accession>
    <name>A0A3B4DKG3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Connector enhancer of kinase suppressor of Ras 2b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNKSR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577381.1">
        <property type="nucleotide sequence ID" value="XM_017721892.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKG3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023611"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012210.2">
        <property type="protein sequence ID" value="ENSPNAP00000023611.1"/>
        <property type="gene ID" value="ENSPNAG00000008148.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442048"/>
    <dbReference type="GeneTree" id="ENSGT00940000164237"/>
    <dbReference type="OrthoDB" id="74412at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06748">
        <property type="entry name" value="PDZ_CNK1_2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01260">
        <property type="entry name" value="PH_CNK_mammalian-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09511">
        <property type="entry name" value="SAM_CNK1_2_3-suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000019">
        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000092">
        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000060">
        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049628">
        <property type="entry name" value="CNK1-3_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010599">
        <property type="entry name" value="CNK2/3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051566">
        <property type="entry name" value="CNKSR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017874">
        <property type="entry name" value="CRIC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844">
        <property type="entry name" value="CONNECTOR ENCHANCER OF KINASE SUPPRESSOR OF RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844:SF12">
        <property type="entry name" value="CONNECTOR ENHANCER OF KINASE SUPPRESSOR OF RAS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06663">
        <property type="entry name" value="CNK2_3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10534">
        <property type="entry name" value="CRIC_ras_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51290">
        <property type="entry name" value="CRIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="11"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CRIC" evidence="7">
        <location>
            <begin position="84"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="215"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="585"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="393"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="467"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="549"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="687"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="789"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="698"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="850"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="874"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1020" mass="115296" checksum="28B674184A47A8A1" modified="2018-12-05" version="1">MALVMEPISKWSPRQVLEWMKGLDDCLLQYVRTFEREQITGEQLLHITHQELEELGVTRIGHQELILEAVDLLCALNYGLETENLRSLSHKLNASAKNLQNFITGRRRGGHYDGRVSRRLPNDFLTSVVDLIGAAKNLLAWLDRSPFVSVTEYSVLKNNIVQLCLELTTIVQQDCTVYETENKILHVCKTLSGICEHIISLSSDSLVSQSAHLEVVHLTNVMPNEGLGMYIKSTYDGLHVITGTTENSPADLCKKIHAGDEVIQVNHQTVVGWQLKNLVNSLREDPSGVILTLKKRPQNTFGSTPGLLKNARWKPLALQPLSTSPCSGSPTPPSTLGSLGVSPGLDTPVGHDVFILSPVFGHREDESERMGFGVFGSESPGFYVDQEGLGGHHFPLLDDETGRNAGIRRRDRTPTRSDPRQISMATEWLAEPQESGVYKRGSRDSDSSLLRFLSDDRILVLQEEAEFSPRDTGWRTRKKSRNPSSPSFPISPSMLAPPVRLDSGPPDGLPFPVSDSNTMPHYHRASDTIRAETDRYASSAGDRQAAASMRKSVHYASLRTKTKKKNRGSQSNSSRRRIACKDLGHGDCEGWLWKKKDASGYFTQKWKKYWFVLKDSDLYWYTNQNDEKAEGFISLPEFRIDRAIECRRKHAFKACHPKIKSFFFATDHLEEMNRWMNRLGLAAIGYTPDDPVPRPDEDYWSESDQEEMDGSMTLKQESTYHRNPSASSSSPFAERHFSSGSTFSHSSAEDSHSSCRSTHRERRSWQDLIETPVGSSGLHYLQTVGGSAVGEGRVMPLSPERLRQATLPARRRRCPPERDGPFPLTDSEEPRPRHNHAHKQRSQSLPRNRGVLHTHTGHAHAHGTLRPRTRLHEEEDEEEEEEGVHEAQQMDPLEELYRSLEQASVSAFGHQRPSTRQEFRRSFVKRCNDPATNERLHRIRALRSTLKAKEGDLSAIGQVLDDPCLTSRKFREWKQRNHELFLDICEFSSVPLEPPTEPNDLSPLSPTLLMHTHSFIETHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DL80</accession>
    <name>A0A3B4DL80_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TAAR1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DL80"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010695.2">
        <property type="protein sequence ID" value="ENSPNAP00000024213.2"/>
        <property type="gene ID" value="ENSPNAG00000001162.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244823"/>
    <dbReference type="OMA" id="GCISITW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15055">
        <property type="entry name" value="7tmA_TAARs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000279">
        <property type="entry name" value="Trace amine-associated receptor 16f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="50"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024213.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="342" mass="38648" checksum="ED3182A2F4C4EE9F" modified="2024-11-27" version="2">MDVRDYRQNITVQYCFPDINSSCRKEVRTGPAYIFLFIFLSCVSVFTVFLNLLVIISISHFKQLHTPTNLLILSLAVADLFVGLIVMPVNIMQLIDSYSCWYLGKMACCLLPVINSLVSSASLCSLVFIAVDRYIAICDPLLYSTRVTVCKTSLFIILGWSSSLFYIIMCLYFNDHFLPSQISTRCEGECVVVIQYSWVIVDLMVSFLAPCCVILILYAVIFNVARHQAKAVRAVRNDASNKQGIKVPSSSETKAAKKIGTVIFVYLACWIPFYLSSLLADSLVSVSVLYTVFGWLVLINSSVNPLIYAIFYPWFRKSVTFFVTCRIFKSSSSRLNLFPKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPF1</accession>
    <name>A0A3B4DPF1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger and BTB domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ZBTB5</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPF1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024819"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009149.2">
        <property type="protein sequence ID" value="ENSPNAP00000024819.1"/>
        <property type="gene ID" value="ENSPNAG00000009577.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444369"/>
    <dbReference type="GeneTree" id="ENSGT00940000160246"/>
    <dbReference type="OMA" id="HHHSEGM"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18196">
        <property type="entry name" value="BTB_POZ_ZBTB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000061">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000250">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF5">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 44 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="17">
        <location>
            <begin position="24"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="646"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="674"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="280"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="443"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="710" mass="75897" checksum="B7A2B95E702577DB" modified="2018-12-05" version="1">MDFPGHFEQIFQQLNYQRVHGQLCDCVIVVGNRHFKAHRAVLAACSTHFRALFTVAESEASMSMIQLDSEVVTAEAFAALVDMMYTSTLMLGESNVMDVLLAASHLHLNTVVKACKHYLTTRTLPMSPPGERGSQHQGSQAAAAANSRLQRSFLLQQLGLSLVSSALNGAEEGSGAGRGPGSVSGSGLVEQRDYPSRRFHKRKQAFSLIRSEAERLRQRPRLSSQSEGTLMEGVGGAVGEELLSPDSHNKVTDEDTKLDVVAGVVASDDSDLLEGRDYRRSMGQEDVQLPSQSDGGRVSRVEQPLLLPHKEEYPDTGRVHGEGVQVKDGGEDEEAQQVVVKSEPLSSPEPIDETSDVTSQAEGSDQVEAVGEKLELSPEGSEHSFSEPQPCADLLLTGSKGHAGGGERGGVGREELSCSEALESTSGFRISSFLSTKSFECSASSSNPAENVPNTTTGDCRLDREPSRLFFGSDSTSMSSTLPPSQNLLSGEAQDVSGLSADALVLRPFHDGIASSSSSSLVNSRGGTAEQLTLSFQRNSLGLHALPRLSRGGSSGFPGYRRIAPKVTPGGASVRTDGGNMQDVASSSSTLLNGISFDGSVPGGQHGNNTNLNPPQLTRASADVLSKCKKALSEHNVLVVEGARKYACKICCKTFLTLTDCKKHIRVHTGEKPYACLKCGKRFSQSSHLYKHSKTTCLRWQSANMSNALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPG2</accession>
    <name>A0A3B4DPG2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017554290.2">
        <property type="nucleotide sequence ID" value="XM_017698801.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037396698.1">
        <property type="nucleotide sequence ID" value="XM_037540801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPG2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026262"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005583.2">
        <property type="protein sequence ID" value="ENSPNAP00000026262.2"/>
        <property type="gene ID" value="ENSPNAG00000011534.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428083"/>
    <dbReference type="GeneTree" id="ENSGT00940000176345"/>
    <dbReference type="OMA" id="ERSRCIM"/>
    <dbReference type="OrthoDB" id="10249393at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="392"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="337"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026262.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="47211" checksum="2EE38A8D9F12CFE7" modified="2024-11-27" version="2">MDGCVVRLHSVSFNSRVEGGSEAFTAQPIRVNFHQLLRTSDAPELLPKVKNQDVLMAVVALLDSTLPDVGSQDIHETVHSPRANTIILLRDHADVLEEFSGRSHPDSQGELVSLKLPEASQDHQSDSYQLSELYSESEDSCEESCGSEETDHRWSWEHFCAGVPEFLAAARKRREIREEAGEDGEIPSSAVGVECLLVAAAAYERKTLLTGEKVLQLSLLATRKRYRRCGVGRYIIELLKSQSVCGNYDALLAHADSDAIEFFSHCGLMDDPLLNDKFREVRDEWANTTLMSYLPPFTTELKSRNPGFSLSLAGLEVEVNLARMTALSAYQQQAVCVTRLVREVKTLRDQLELQREEVDMLHNELEREKEKRHKIEQMFLEYKLRKTWQLLEGRDSDRGSQTDSGPTSPAEAKPEPGHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DR28</accession>
    <name>A0A3B4DR28_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Neural cell adhesion molecule L1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Growth cone</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571075.1">
        <property type="nucleotide sequence ID" value="XM_017715586.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017571076.1">
        <property type="nucleotide sequence ID" value="XM_017715587.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017571077.1">
        <property type="nucleotide sequence ID" value="XM_017715588.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017571079.1">
        <property type="nucleotide sequence ID" value="XM_017715590.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397445.1">
        <property type="nucleotide sequence ID" value="XM_037541548.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000006385.2">
        <property type="protein sequence ID" value="ENSPNAP00000025915.2"/>
        <property type="gene ID" value="ENSPNAG00000011205.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438063"/>
    <dbReference type="CTD" id="30656"/>
    <dbReference type="GeneTree" id="ENSGT00940000157506"/>
    <dbReference type="OrthoDB" id="6244967at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000057">
        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002563">
        <property type="entry name" value="Neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000367">
        <property type="entry name" value="Neural cell adhesion molecule L1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000078">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000347">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026966">
        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF36">
        <property type="entry name" value="L1 CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043780830" description="Neural cell adhesion molecule L1" evidence="19">
        <location>
            <begin position="38"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1138"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="54"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="142"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="248"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="341"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="434"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="524"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="622"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="723"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="822"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="941"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="1039"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1167"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1241"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1168"/>
            <end position="1205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025915.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1288" mass="144452" checksum="84750A01DC6CD700" modified="2024-11-27" version="2">MPPAQCQPAATRGQLGAPSRQLLFSVFLLLLLGQTQGGMHIPKDYVINDLKEPPVITSQPESYTAFYLDDVLLKCEAKGNPAPKFRWVKDGKLFEEGSYSSGVITANESKDLKYYQGTYRCFASNDLGTAMSDVMELITEPPPILPKERRVRKTVNEGDDVVLHCNPPNSSVVPYIHWMDKKLLHIKQNERVTIGLDGNLYFANVLVTDTRDDYTCNAQYVPARTILPKEPISLHVNPSNMVAKIRRPRLHRPEGTHSHYLALRGQTLTLECIPHGLPTPTVTWERKDGPLSLDRASVLNYDRWLRFEEILESDDGEYSCTANNSHGFISHTYMVEVKAAPYWTKKPQSQLYAAGETVRLDCQAEGIPTPQITWKINGVPILDTDEEWRRQVTGGTLILSSVSYSDTAVYQCEASNIYGSSLINTYIHIIELPPQMLTENQEVYRVTEGSSASLNCSTFGSPRPMISWETEGDEPVLSNPKMSQMTNGTLLITNVSREDAGPYFCSVKNSSLSITALLDVLNRTQIISPPRHLQVLRGQSGVMQCQYEVDLQLKDPTIVWRKNGQKITTSAQDDKYTVFENGSLKITDVQHKDTGRYSCEVITELDMDIAIGSLTVVDKPDVPHSLQLSEKKSRSVTLSWTPGNENNSPVLDYVIEMREDVHSEASGWKVVEQISAEINRIEIPLLPFCTYHFRVAAINEIGMSDFSMPSESYATPPAKPDAYPKNVMSESTDPDSMVITWEEMKRKHFNGPDFRYKVFWRQSAGQQLNWEHVYVSHPPFIVNNTGTFTAFEIKVQAVNRLGEGPSPTSEIGHSGEDFPEKPPTRINVSQINGTAVNVTWNAVSRESVRGHLQGYKIYLRRLHPQGIHDHRGKRELALRDMEREEESRVIVVYGDRKEQAVLSGLEFYSDYQLSIAAFNKKGDGPQSEPYSFKTPEGVPGPPSIWKLESPSKTELTLYWRPPRKINGVLKGYILNYQEFIENSPSTLEYMRIDDPKKTHFTLSNLNPQRHYFFDLRALTNAGDGDPARVNGSTLLDGEPPSSVNATVGETFVNLSWVPSHRRRNIGFTVHYRKTVGGQWEESEQVNSTQAFYQLQGLQPGTQYVLEIRLSNETFWMQELQTNGPELAVVQNGFASEGWFIGLISAVVLLLLILLILCFIKRSKGGKYSVKDKEDGRVDSEARPIKDETIGEYRSLESSDNDEKRSISQPSLCVESKRASDDSLAEYGDSVDIQFNEDGSFIGQYSGRRDHHGQAGDHDSSGATSPTNPNMPPPSMSFPSSVTGILGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DRI0</accession>
    <name>A0A3B4DRI0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037395205.1">
        <property type="nucleotide sequence ID" value="XM_037539308.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000025701"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006990.2">
        <property type="protein sequence ID" value="ENSPNAP00000025701.2"/>
        <property type="gene ID" value="ENSPNAG00000010902.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413098"/>
    <dbReference type="CTD" id="555303"/>
    <dbReference type="GeneTree" id="ENSGT00940000164866"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058210">
        <property type="entry name" value="SACS/Nov_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047352">
        <property type="entry name" value="P_loop_sacsin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46919:SF2">
        <property type="entry name" value="SACSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46919">
        <property type="entry name" value="ZINC FINGER, C3HC4 TYPE (RING FINGER) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25794">
        <property type="entry name" value="SACS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="4155"/>
            <end position="4272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025701.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="4282" mass="489985" checksum="D85B2888F783BA52" modified="2024-11-27" version="1">[encoded data, 4282 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DS23</accession>
    <name>A0A3B4DS23_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017557471.1">
        <property type="nucleotide sequence ID" value="XM_017701982.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DS23"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026260"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005586.2">
        <property type="protein sequence ID" value="ENSPNAP00000026260.1"/>
        <property type="gene ID" value="ENSPNAG00000011548.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429902"/>
    <dbReference type="CTD" id="325255"/>
    <dbReference type="GeneTree" id="ENSGT00940000164184"/>
    <dbReference type="OMA" id="WENDTRQ"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01863">
        <property type="entry name" value="Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000430">
        <property type="entry name" value="Probable Ras-related protein Rab-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="200" mass="22594" checksum="9FEE3A55C73F80D8" modified="2018-12-05" version="1">MDDGILTTLKILIIGESGVGKSSLLLRFTDDMFDPELGATIGVDFKVKTIAVDGNQTKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVTRRETFSKLSTWLNELETYCTRDELVKMLVGNKIDKEDREVDRNEGLKFARKHSMLFIEASAKTRDGVECAFEELVEKILQTPGLWESERKTRVHLSEQPTQPTSGGCYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DU88</accession>
    <name>A0A3B4DU88_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Afadin- and alpha-actinin-binding protein-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017568275.2">
        <property type="nucleotide sequence ID" value="XM_017712786.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DU88"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027025"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003635.2">
        <property type="protein sequence ID" value="ENSPNAP00000027025.2"/>
        <property type="gene ID" value="ENSPNAG00000012418.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436357"/>
    <dbReference type="GeneTree" id="ENSGT00390000007688"/>
    <dbReference type="OMA" id="QDMLRCE"/>
    <dbReference type="OrthoDB" id="312015at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052300">
        <property type="entry name" value="Adhesion_Centrosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46507">
        <property type="entry name" value="AFADIN- AND ALPHA-ACTININ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46507:SF3">
        <property type="entry name" value="AFADIN- AND ALPHA-ACTININ-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027025.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="51644" checksum="24FA1EE259826707" modified="2024-11-27" version="2">MAYRHGGRPPVSSCRVQEASEIQTTHVSLQASPPCSASYGILQEEKKEKNSSLQEQLIEKDQYITRLQETLRLEREKNVKLQSRFNQQGLELRRREQHANRMREKLFQFTDRHRDRGASIEVINALPKPSGRREPVSRTARADGRKEQAVQLMLERREAELRESMKLRHSLTTLLHALRTDMQQTLQDSVDAEEEEQQQQQVDTSWLVQSEQSLGDHVTGGVVLEWTEVKKRLRELQSKSTVAVRTDQEKFLAQLEEELEQSRELVRVQQQLLQDNVDSPLPAALIDSYYLEEWERLQDQWVELKHQRRSFQQERQAFTEAAIRLGHERCVFQQQKAALMKQQFLTTSPIMKTSHSNRRESTALGELRVAAPDSLALSPCAPITPSSEGSEVTPRSAQNIMTPSTPELYSVLQLNYCRSVESPPPFQKEPLEGVFSPEHQNMDDWPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUE5</accession>
    <name>A0A3B4DUE5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="4 14">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PDIA2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4 14"/>
    <dbReference type="RefSeq" id="XP_017572901.1">
        <property type="nucleotide sequence ID" value="XM_017717412.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUE5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027088"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003471.2">
        <property type="protein sequence ID" value="ENSPNAP00000027088.1"/>
        <property type="gene ID" value="ENSPNAG00000002275.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439166"/>
    <dbReference type="CTD" id="64714"/>
    <dbReference type="GeneTree" id="ENSGT00940000165626"/>
    <dbReference type="OMA" id="REDYVWS"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000023">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000027">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000042">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF93">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11 12" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017102657" description="Protein disulfide-isomerase" evidence="14">
        <location>
            <begin position="23"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="37"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="381"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="504"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="514"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="540"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="563"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="12">
        <location>
            <begin position="432"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="577" mass="65811" checksum="E363BEEB601C45D0" modified="2018-12-05" version="1">MKLLRVLLLSLLLCALCSRAEEEKVEEGSEEEKDEKETEAETEPEEKKKTDEITEEKDVLVLHSKNFARALSENKYLLVEFYAPWCGHCRALEPIYAEVAGQLKSESSPVRLAKVDATEEKELAEEFDVDSFPTLKFFKDGNRQNVTEFTGKRTAKGIMQWLQRRLGPSATLLENAKDVKKLLDAHDVVVVGFFKDLDGEEAKVFYDVALDTISLNFGITSSPELFKKYELQKDSVVLFKKFDDKRADMPLPEDGKLDKDDLITFIQSNSLELVITFSDENADKIFGSKVHNHLLLFINTTIESQNALLDDYRDAAGEFKGKVLFIKIDVNQQVSHVMKYFGISASDTPILRLINTDTVKKYAMKEKDINEDTVKAFCQDMLDGKLTPHLMTQEIPEDWDKNPVKVLVGKNFEQIAFDETKNVFVEFYAPWCSHCKELAPVWEKLGEKYKDHENIIIANMDATANEVEDVSVQGFPTLKYFPAGSERKVIDYNGKRDFETLSKFLDNGGVLPEEKEDDDDDDDDDDDDDEDKDEDDDKDEDKNEEKDQKGSEEEEGSSESTEDLEKPSNKSSKKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXI8</accession>
    <name>A0A3B4DXI8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZNF407</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXI8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027771"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001703.2">
        <property type="protein sequence ID" value="ENSPNAP00000027771.2"/>
        <property type="gene ID" value="ENSPNAG00000013303.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156446"/>
    <dbReference type="OMA" id="PWCDYSN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000969">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001030">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001109">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001819">
        <property type="entry name" value="zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002581">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000395">
        <property type="entry name" value="zinc finger protein 513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF102">
        <property type="entry name" value="ZINC FINGER PROTEIN 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="873"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1057"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1085"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1115"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="1143"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="169"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="655"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="805"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="174"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="431"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="655"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="679"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="694"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="812"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027771.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1545" mass="172566" checksum="1D91174E0A830EB2" modified="2024-11-27" version="2">MPQLDQLKRKIKSGKEMAKNIKAGPSFSEPDTEGAPSEPKRPQTTNVGGADRSLSEENEGTQDDSESTQADPPGYLGENDICACSFCDFVAKNPTALKVHSKRKHSQRALSSHAENVSQKNCGESALSDVKEMENILYTDREDAYQPEHLAVPTQPEMTLLMSPKNVPLKQSERTSSYQTENSPDQSPGNEAETTQNHCDQSAGDISSPTRILLEMIDPSLGDNRKYGTCEMKTVDEGTENGQVGLTCSSDVAGKRTSKRCPKPKVLHACSYCGHEFRDRPSLEMHVKRRHTKVMNYFCKFCSYSCVAKCDYEKHCMSNKHRRKILEKRETSFEVDKVDSTGGTSEHQCSSCSFTASSSILLVEHMKLQHGAENRLHCQMCHFYTCTAEAMQAHLEAPLHLQAAQEKNTSPLYQHCVEKVLVILSNEQREEEQREEMSEQGGKREVEPQSVSKYVPEFQETANFSTEENFEGSITHRRKRGRPKSSHVTTCSHCGLIASNPTNLSVHIRRRHSRLYSFFCKLCNYYCVTKGDMDRHCETKKHIKRAQADGAAEAVVCLDTEQAVQVSEDEVVWAAQMKGATGDEKDSDEMEQTLSEAPCKKNKYDLVNSCSHCDFVAHSMASLRLHVRRKHKRDFEFVCLACSYYAVTHREMSRHMATEKHKQKRDTYLQQRDRKDSAEVNQHLASANQTTFTEDAHQNMKKPSAKETGDSAEALERTDGVENDMTLERVTKTKEPNQSSAENMCGLVQSNSPGEHVMEYTEEDDEDEDEESLEVKADGENLRLRATPFDKCIFHLKAQTEALQEVTEGDSKPTTRASSLQGVKNQRLAESAGTKGAAPNPRIRCEDCGFLADGLSGLNVHISMKHPSKEKHFHCLLCGKSFYTDSNLQQHLSSAAHLRNEQGSVEELPEGGASFKCVRCSELCGTEQELFLHIKEKHEELLREVNKYILEDTEQINRERQENQGSVCKYCGKVCKSSNSMAFLAHVRTHTGSKPFMCKICNFATAQLGDARNHVKRHLGMREYKCHVCGWAFVMKKHLNTHLLGKHGLGQPKERKYECELCERSFSEKWALNNHMKLHTGDKPYKCSWESCHYAFLTLSAMKDHYRTHTGEKSFLCDLCGFAGGTRHALTKHRRQHTGERPFRCQLCSFASTTQSHLTRHKRVHTGEKPYRCPWCDYRSNCAENIRKHILHTGKHEGVMMYNCPKCDYGTNAPMDFRNHLKENHPDIENPDLAYLHAGIVSKSFECRLKGQGASYVETESAFAPDEGRLGSEALQQVIIIQGYGEAGAMEQELEESAAATLHTLAMTHTHTQLQVAQVLHITEDGQVIPCHKVAPSGTPLPEGATQYLLVEGQEEGQSSPEQAGVSQVLSESSTALDALLCAVSEIGQQGSAGETEIITSEQEGHAQGEGHEEVQVYGEVVEGVEGEGAESVDVVTQVVGHGNQDTPEGVVQEVLQFAASQLMMKEGLTQVIVNDEGTHYIVTELDDSTLQVEGTVYAQSEGAESGETSQPHEGMGPKELIPCTIWLHPLTQELEQAGLGSDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZC8</accession>
    <name>A0A3B4DZC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tumor necrosis factor-inducible gene 6 protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">TNF-stimulated gene 6 protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Tumor necrosis factor alpha-induced protein 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TNFAIP6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts (via Link domain) with inter-alpha-inhibitor (I-alpha-I) component bikunin. Interacts with ITIH2/HC2; this interaction is required for transesterification of the HC to hyaluronan. Interacts (via Link and CUB domains) with ITIH1. Chondroitin sulfate may be required for the stability of the complex. Interacts (via Link domain) with various C-X-C and C-C chemokines including PF4, CXCL8, CXCL11, CXCL12, CCL2, CCL7, CCL19, CCL21, and CCL27; this interaction interferes with chemokine binding to glycosaminoglycans. Interacts (primarily via Link domain) with BMP2; this interaction is inhibited by hyaluronan. Interacts (via both Link and CUB domains) with TNFSF11. Interacts (via CUB domain) with FN1 (via type III repeats 9-14); this interaction enhances fibronectin fibril assembly. TNFAIP6 may act as a bridging molecule between FN1 and THBS1.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547692.1">
        <property type="nucleotide sequence ID" value="XM_017692203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZC8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029767"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017193.2">
        <property type="protein sequence ID" value="ENSPNAP00000029767.1"/>
        <property type="gene ID" value="ENSPNAG00000016088.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424290"/>
    <dbReference type="CTD" id="7130"/>
    <dbReference type="GeneTree" id="ENSGT00940000157201"/>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="F:hyaluronic acid binding"/>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
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        <property type="entry name" value="TUMOR NECROSIS FACTOR-INDUCIBLE GENE 6 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="LINKMODULE"/>
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        <property type="entry name" value="C-type lectin-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3 9" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017439088" description="Tumor necrosis factor-inducible gene 6 protein" evidence="10">
        <location>
            <begin position="23"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="12">
        <location>
            <begin position="41"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="11">
        <location>
            <begin position="140"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="63"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00064571"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069597"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00077610"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00081068"/>
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        <source>
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            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
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            <dbReference type="PROSITE" id="PS01180"/>
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        <source>
            <dbReference type="PROSITE" id="PS50963"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029767.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="264" mass="29779" checksum="A5BCE46D3A7BEEE6" modified="2018-12-05" version="1">MIAVHIIIVVFALGLILKETQAWGYKNGILHNSIWLEQAAGVYHRESRKGRYQLTYKEAKAVCAYEGGTLATYDQLEAARQIGFHVCAAGWFAKGRVGYPIVKAGANCGFGKVGIVNYGYRLNKSERWDVYCYNPNSKECGGVHTDQEKVLQSPGYPDEYPDEQICYWHIRLRYGQKIRLHFLDFDVEDDTDCLSDYLEIYDSYDDISGFAGRYCGDELPEEFISTGNVMTLKFRSDSSVSAGGFQLHYIAVDSSELSNNETST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZD3</accession>
    <name>A0A3B4DZD3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSPNAT00000000158.2">
        <property type="protein sequence ID" value="ENSPNAP00000028364.2"/>
        <property type="gene ID" value="ENSPNAG00000014032.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Leukocyte immune-type receptor TS32.15 L2.5a"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig_Fc_receptor"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
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        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IG"/>
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    </dbReference>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="Ig-like" evidence="4">
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    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
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        <location>
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            <end position="426"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028364.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="434" mass="48507" checksum="1DB5308B9281836D" modified="2024-11-27" version="2">IPKPAVSIQPDHQVFRGETVTLTCDIQGGGVSTWQYSWFKDDPYRRVSNKQQYSISSVTASDGGKYTCIGAVRGTSRYSHYSEAVTLTVSERPKPAVSIQPDHQVFRGETVTLTCDIQGGGVSTWQYSWFKDDPYRRVSNKQQYSISSVTASDGGKYTCIGAVRGTSRYSHYSEAVTLTVSGKPKPTVRVDPQRSVYTGDTVTLTCKVESTGWEFLWYKDSKELQPQSPVGKDTNTVRVTVSHAGRTEFECAARRGAYTTQTSAAAVITVTARPKATVTINPADPVFIGETVTLTCHIGSGRHWQYYWFKDSKPLSEAQQRKEYTISKVAESHRGVYICNGTQSTQPRYSETSDAVTLTVSDKRDLLRANPKSGWRQSRVREPIRRDRGADMTDTRIKPTTQTEIETKQTSNTDQYKDQRKQRADSGLKYTPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0F0</accession>
    <name>A0A3B4E0F0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia and flagella associated protein 99</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CFAP99</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017550041.1">
        <property type="nucleotide sequence ID" value="XM_017694552.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0F0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028781"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015137.2">
        <property type="protein sequence ID" value="ENSPNAP00000028781.1"/>
        <property type="gene ID" value="ENSPNAG00000014516.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425683"/>
    <dbReference type="CTD" id="402160"/>
    <dbReference type="GeneTree" id="ENSGT00500000045231"/>
    <dbReference type="OMA" id="VQDGRYC"/>
    <dbReference type="OrthoDB" id="10262255at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039341">
        <property type="entry name" value="CFAP99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="549"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="640"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="665" mass="78164" checksum="6433CC5039FE4FF2" modified="2018-12-05" version="1">MNYRELVREAITLLDKYEPDKQSVDSFIEDSAKTLGNHPSADQTFISDTVYGCVEHRKLLDIVVNVFYDQHGKSLFKADRNKFVVVCYLSMFHLEDLGLEQFSRIIKSLDTSKMHKFLSFFFNVTNLTTWIHGEWSQIYDATYVEHKWIAPLLRWQAQIEGLLDYLSKKMTKGSLPRKSPKKCTQSQEFDLTKPKPRPLPAPEPVPQQEKTKPVPLSTHRAPKEPQLLEDIKQKNHLKAQQVLYEANIQQFRCANQQKSEKTQNTVSQIQQNFNSKLKFDSVYSSGTPATQKMNILPVRLNTTAILREGALYNRQMEEELNRLEHLMQGANEPSAFLQWQKEMREKDLQEELAQVERRRLEGKISYEEALLARQRVLELNQHKAQLKKEETAELMRKYAEKRLREEEEMKELVQQVASGHKNSKAAKIKLQEIKQRIVKEVSEQSRELLRQALEEAQAELSRRFELIRQIRAIESVPHNRHMFLDDTQTGGHELLCEMSLVELRERLARLREEEQREQEEKRHKIQEEKQSREQLLLEQLDTIAMRRTAARHATTQKQEEKKKRRKLQEAVSMDERVMALQKTLEMKQQERQNKRESEKGKVNISKQIATQKVKSYNQEKQALEEQHWLQLEQALERQVRREASSRNTSGLNYGKATNDLFPSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1Q5</accession>
    <name>A0A3B4E1Q5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transmembrane serine protease 3a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1Q5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029670"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017013.2">
        <property type="protein sequence ID" value="ENSPNAP00000029670.2"/>
        <property type="gene ID" value="ENSPNAG00000015980.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15494">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="31"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="152"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029670.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="44803" checksum="D63BC467622C7048" modified="2024-11-27" version="2">LKNTRKYVIWPGVTFTPVFSVAVGLSCMGKFRCGSSHCISSSAQCDGHVDCEHGEDELGCVRVSGKSSVLQVLSDGVWSTVCSEDWDHELGLSACKQLGYTSSWWPYSDSVVMADKLTLSFSLCSKTECSSGEVITLKCLDCGSRPQFSTRIVGGNLSREGQFPWQVSLHFQSEHLCGGSIVAPRWILTAAHCVYGFAFPQLWTVLVGLTEQPVSGAKYLSVEKIIYHARYRPKGLDYDIAMLKLVEPLTFNGYIEPICLPNFGEEFEDGKMCWISGWGATEDGGEASVSLHSARVPLISAKACSQPEVYQGYISPGMICAGYLEGGTDSCQGDSGGPLACEDSSVWKLVGATSWGQGCAERNKPGVYTRITQALAWIHQQMEVREITGTNNSMKVKAKGKLIYTEKILQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E695</accession>
    <name>A0A3B4E695_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="15">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ACSS1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4E695"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030759"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019106.2">
        <property type="protein sequence ID" value="ENSPNAP00000030759.2"/>
        <property type="gene ID" value="ENSPNAG00000017713.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063174"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000020">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
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        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
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        <location>
            <begin position="302"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00003754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023052"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051231"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030759.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="665" mass="72706" checksum="6445A87FCD3FC604" modified="2024-11-27" version="2">MRKMLSLISSALRLSAGRTGAELVNESLAVQRLLAAQGEYVDMTLDPDPPQHPKIHPTPVSGTHRVKRDPPSETLAPLVPVDLISVNEAVEPDWTETLRSERSHRDKPVVTGPPANGSASVGPEVILDVSTKDEMLTALANTELLISHPLDGAGDAVVQQAAVSESAITELLVSSAALEAPSPVDGAAGQKAPDRTVWATAQTPALPSAELTPPLTPSSMVRRSRNVDLTRHPESVHSQAGHAAIPEPQPAGSRVVVSARTYADFTPQATFDIKKCDLHKLEEGPPVQAVLTREEGLQYYRTMQIMRRMELKADQLYKQKIIRGFCHLYDGQEACAVGIEAGINLTDHLITAYRAHGYTLTRGGTVREIMAELTGRRGGIAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGVALACKYQGKNELCVCLYGDGAANQGQIFETYNMASLWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAADHCRSGKGPIIMELQTYRYHGHSMSDPGISYRTREEIQEVRSKSDPISMLKDRMLNNNMASVEELKEIDVEVRKEIEDAAQFATTDPEPPLEDLCNHIFHNEPPLEEIDVEVRKEIEDAAQFATTDPEPPLEDLCNHIFHNEPPLEVRGTNPWVKLKSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E6P5</accession>
    <name>A0A3B4E6P5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SLAIN motif family, member 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SLAIN motif-containing family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566327.2">
        <property type="nucleotide sequence ID" value="XM_017710838.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E6P5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019411.2">
        <property type="protein sequence ID" value="ENSPNAP00000030909.2"/>
        <property type="gene ID" value="ENSPNAG00000017971.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435190"/>
    <dbReference type="CTD" id="560157"/>
    <dbReference type="GeneTree" id="ENSGT00390000017860"/>
    <dbReference type="OMA" id="VGEPESW"/>
    <dbReference type="OrthoDB" id="8819875at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026179">
        <property type="entry name" value="Slain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22406">
        <property type="entry name" value="NASCENT POLYPEPTIDE-ASSOCIATED COMPLEX SUBUNIT ALPHA, MUSCLE-SPECIFIC FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22406:SF2">
        <property type="entry name" value="SLAIN MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15301">
        <property type="entry name" value="SLAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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    </feature>
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            <begin position="20"/>
            <end position="54"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
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            <begin position="325"/>
            <end position="338"/>
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            <end position="360"/>
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    </feature>
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            <end position="384"/>
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        <location>
            <begin position="534"/>
            <end position="569"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006652"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030909.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="66294" checksum="2A6C6F33F593FF82" modified="2024-11-27" version="2">MEAAVLNLQMMADVNGNSKVMDAELEVKKLQELVRKLERQNEQLRTRANTANSCPSAGHLCSNSSCSGLLTAGQFYTGNYSVSSPSATGSFGPHTTEELYPYFHPQREAENNGCEQTALDEAEMLDLSALLPIDGQAEDTWLYVSPKAKLYPQYPLSPLQWCRHVLDNPSPEVELAKRSLCYRLEQAKRCRGVLSGYASSSLAYSPVDGLSSLSTPVKPLTKPTLTEQTAPSSPSSQCLLQQPLTGRSCGGLAERSPSFLYHTATQNYSRPHAAVSPQSSTDNDFSTSELEDDSISMDYKLQDMTDVQVMARLQEESLRQEYATTSATVARRSSSFSVHSGLRRPGRSTVELEEEEECDDLPPPQPRLFRTGSMQRSLSHSYNLSSPRDPRRSPSSPQYLSSLSYQQLSNPSPSTTSYTANEAQSYRTSTDKLRRSMPNLIRAPSMLSVPSVPSVAAPASHTAFPSFSSSSSSLRNSQSFDSSSGLARLQTAIPSPGQLQQRVQSIGNFSMATRQPLKATAYVSPTIQGPTTMPSSISLNSLSSSGIPLPSKPSTSSSTSRSALPRPASFIGTTGTSRSKIAQPVRSLLTPPKSIAALTTLRDTSWKDGCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7D1</accession>
    <name>A0A3B4E7D1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017543796.1">
        <property type="nucleotide sequence ID" value="XM_017688307.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017543798.1">
        <property type="nucleotide sequence ID" value="XM_017688309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7D1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032522"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022521.2">
        <property type="protein sequence ID" value="ENSPNAP00000032522.1"/>
        <property type="gene ID" value="ENSPNAG00000020506.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415286"/>
    <dbReference type="CTD" id="555804"/>
    <dbReference type="GeneTree" id="ENSGT00940000154317"/>
    <dbReference type="OMA" id="NHYDPHS"/>
    <dbReference type="OrthoDB" id="8044756at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF20">
        <property type="entry name" value="ADIPONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017409133" description="C1q domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="8">
        <location>
            <begin position="527"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="27"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="370"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="660" mass="63075" checksum="2BF900D28271909A" modified="2018-12-05" version="1">MDLQVTSVLLLLLVLAEGTPERYYHVKKNPYPTKSHPVAGTPGSPGAPGEPGPPGPPGSPGQPGASGIGHPGPVGPPGPPGPAGYSAPGKSGSPGAPGKPGANGLPGERGIPGPAGPQGARGAPGSPGSPGPAGYSAPGKPGPHGLPGAMGPRGEPGEKGLPGIPGAPGHKGESGHGIPGSPGGPGPMGPVGPPGQPGQPGIGKVGATGYPGEPGKPGLSGRDGSPGPIGAPGLKGHTGAPGIGAPGKPGQNGSPGLPGPSGQKGPQGPAGQPGAPGLPGVGKTGAPGFPGDRGAPGSPGTTGQKGEPGPTGFTGQPGAIGPIGPAGPQGARGFQGEPGPAGPKGDHGLVGAPGPRGAKGEQGAQGFTGKPGIPGAAGPTGAPGPVGHQGPKGEAGFTGAPGIPGAIGPAGVKGHPGPQGIPGSRGENGIPGARGPVGSVGPAGAPGPKGHPGLPGAPGPAGITAKGLSGPQGPPGIPGARGHDGLPGPAGPPGPPGPPGQIVYHHEKSMPIKSHEYEVMPLSHELVKPPVSAFTALLTSAYPSAGTPLVFNQIVYNGENHYDPHTGVFTCQIPGLYYFAFHMHVNGANALVALYKNSEPVLFSYDEYNKGFLDQMSGSAVLLLHSGDRVYVQVPDEQSNGIYADDNVHCSFSGFLIAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E895</accession>
    <name>A0A3B4E895_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DNAJC28</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017550660.1">
        <property type="nucleotide sequence ID" value="XM_017695171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E895"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031479"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020471.2">
        <property type="protein sequence ID" value="ENSPNAP00000031479.1"/>
        <property type="gene ID" value="ENSPNAG00000018836.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426022"/>
    <dbReference type="CTD" id="54943"/>
    <dbReference type="GeneTree" id="ENSGT00940000154065"/>
    <dbReference type="OMA" id="EWISLGK"/>
    <dbReference type="OrthoDB" id="1922282at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052573">
        <property type="entry name" value="DnaJ_C_subfamily_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39158:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39158">
        <property type="entry name" value="OS08G0560600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="42"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="376" mass="43888" checksum="394A581121B92A4B" modified="2018-12-05" version="1">MSCSVLLRLHYGDLRRVLLPICKAPTLRTLCSDTRLSRSLRESYRLLQLPEDGDSSPAEVREAYVRMAKLYHPDSGAPTADSALFGHIEEAYRAVVAHMARRGSTSPYAQMEEDEEDKNKSYAPQHRQYLSFEGVGSGTPSQRERQYQQFRMDRATDQVLEYRRKRMEQAAAEEGAMVTRDARVRSKQIKITQAMERLVEDLIQESMARGDFHNLSGAGKPLRKFDHNPYADPMTHNLNRILIDNGYQPEWIVTQKEIRETITKMRERLQDARSRLGIPMRHSEQLRWKEHCAAFADELKKLNKKVDNFNLVVPLMSLQMVHFNMTKEVEKVLKADQEYRKQKEKERKCMEEREKADLSQQNVRQGLIMWMQSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EBJ5</accession>
    <name>A0A3B4EBJ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LPCAT4</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539462.1">
        <property type="nucleotide sequence ID" value="XM_017683973.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EBJ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033912"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025230.2">
        <property type="protein sequence ID" value="ENSPNAP00000033912.1"/>
        <property type="gene ID" value="ENSPNAG00000022881.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412089"/>
    <dbReference type="CTD" id="254531"/>
    <dbReference type="GeneTree" id="ENSGT01030000234574"/>
    <dbReference type="OMA" id="HEYPHPF"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036151">
        <property type="term" value="P:phosphatidylcholine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF7">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE LPCAT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="518" mass="57468" checksum="E3D1783766F65C94" modified="2018-12-05" version="1">MERFQRCRGASTTTTAHPFVHDVRPTGMQKVLMILLGCIFFPIRIALAVLFFLLVWPIARLRLAGLSEADRARPVEGWRRWLFHPIVLFLSRGVFFFTGFLWIKVKGHQATQQEAPVLAVAPHSGFLDMLVLCVSGLPTVVSRSENANLPVIGALLEFNQSVLVSRKDPESRRKCVTQIKERLTSSGYWPQMLMFPEGTTTNGQALIKFKPGAFLAGVPVQPVLLHYPNKLDTVRWTWKGISWLECLWYTTSQFYTNVTVEFLPVYMPSQQEKENPELYADNVQKLMAKALGVPATDYVMEGLFPATKLGGLSLPLESPAGETLKLLKRHSFSSAQVETVMNTMIDYCHSKQHSMVTVKQLASLLGLADEKTAAKICSLYSKNDTLDLRQLCLSVCTTAGLRSPEALLHTAFMLYDSDGDGLLSAVDLSGLMAALVGVPQYNIAEMYTELTTRGQPTEAALYDLLTTHPTYQKFLNECFQSEGAGLAMADGDHFLKGKASSNNNGIHNGNGVANKKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFR0</accession>
    <name>A0A3B4EFR0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFR0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034573"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026537.2">
        <property type="protein sequence ID" value="ENSPNAP00000034573.1"/>
        <property type="gene ID" value="ENSPNAG00000023977.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012710"/>
    <dbReference type="OMA" id="QVNPWWE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF3">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017176282" description="C2 domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="131" mass="14650" checksum="05A158CB505055B6" modified="2018-12-05" version="1">MAFSPRLVYLAVLVLASHVMLSNAAVRVSRLRATNLPGDPIGHPDPYVKVWCDSTFGGMTEFQQNNANPIWTAEFNFPNCAAKDILKLEVWDKDTHYDDLLETCYAHVQSGTHANECTVGKGSLRYSYELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHB2</accession>
    <name>A0A3B4EHB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin-protein transferase MAEA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Macrophage erythroblast attacher</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MAEA</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017547613.1">
        <property type="nucleotide sequence ID" value="XM_017692124.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHB2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035250"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027985.2">
        <property type="protein sequence ID" value="ENSPNAP00000035250.1"/>
        <property type="gene ID" value="ENSPNAG00000025102.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424228"/>
    <dbReference type="CTD" id="10296"/>
    <dbReference type="GeneTree" id="ENSGT00940000153203"/>
    <dbReference type="OMA" id="ANHETAR"/>
    <dbReference type="OrthoDB" id="1933455at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16659">
        <property type="entry name" value="RING-Ubox_Emp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044063">
        <property type="entry name" value="ZF_RING_GID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN TRANSFERASE MAEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51867">
        <property type="entry name" value="ZF_RING_GID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0265">Erythrocyte maturation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CTLH" evidence="11">
        <location>
            <begin position="160"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="RING-Gid-type" evidence="12">
        <location>
            <begin position="315"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="RING-Gid-type" evidence="10">
        <location>
            <begin position="315"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="397" mass="45415" checksum="95309520DC5D3D77" modified="2018-12-05" version="1">MAVQETAAQLSMALKVQEYPTLKVPYETLNKRFRAAQKNIDRETSHVTMVVAELEKTLSSSFPVVDSVVSLLDGVVEKLSALKRKAAESIQAEDESAKLCKRRIEHLKEHSSDQPANVSVWKRKRMDRMMVEHLLRCGYYNTAVKLARQSGIEDLVNIEMFLTAKEVEESLERQETATCLAWCHDNKSRLRKMKSCLEFSLRIQEFIELIRQNKRMDAVRHARKHFSQAEGGQLDEVRQVMGMLAFPSDTHISPYKDLLDPGRWKMLIQQFRYDNYRLHQLGNNSVFTITLQAGLSAIKTPQCYKEDGTSKNPDCPVCSKSLNKLAQPLPMAHCANSRLVCKISGEVMNENNPPMMLPNGYVYGYNSLLSIRQDDKVVCPRTKEVFNFSQAEKVYIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHT0</accession>
    <name>A0A3B4EHT0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MYADM</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHT0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028260.2">
        <property type="protein sequence ID" value="ENSPNAP00000035303.1"/>
        <property type="gene ID" value="ENSPNAG00000003888.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182933"/>
    <dbReference type="OMA" id="GLCTWDK"/>
    <dbReference type="OrthoDB" id="8757663at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047123">
        <property type="entry name" value="MYADM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068:SF3">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER MYADM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="5"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="143"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="293" mass="32261" checksum="DC7666CB71C9FA75" modified="2018-12-05" version="1">MAMVFRSSPLLWTRLVALIFSCVAFSVALYGAQLTHGTGDWCIFCWSFSFVGTLLVVLVELCGLQTRIPVSWKNFPITFACYASLLCLSASIIFPLYFLKGYSGRDEVMNCRIVSCVFSCLATVAYLSEVSLSKARPGEVSGYMATAPGLLKVCETFVACIIFVFINDPISYNQNAATKWCLAVYCICFILSAGIIVLCVAEWTGCLPFPFARFLSTYALLAVALYLSATIIWPLYKFDDKHGGTSHRQSSCGSMAGLCWWDKLLAIAVLTALNFILYLADLIYSARLVFVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJE4</accession>
    <name>A0A3B4EJE4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ITPR3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Endoplasmic reticulum membrane</location>
            <topology evidence="1 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="21">Composed of a large N-terminal cytoplasmic domain (CD) followed by a juxtamembrane domain (JD) and a transmembrane domain (TMD).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the InsP3 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576783.1">
        <property type="nucleotide sequence ID" value="XM_017721294.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000017056"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029521.2">
        <property type="protein sequence ID" value="ENSPNAP00000035888.1"/>
        <property type="gene ID" value="ENSPNAG00000037452.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108441650"/>
    <dbReference type="CTD" id="3710"/>
    <dbReference type="GeneTree" id="ENSGT00940000157078"/>
    <dbReference type="OrthoDB" id="300855at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048706">
        <property type="term" value="P:embryonic skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000002">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000028">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.30:FF:000001">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor, type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.30">
        <property type="entry name" value="IP3 receptor type 1 binding core, RIH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF1">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00779">
        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 21" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="6 21" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="19" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17 21" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2194"/>
            <end position="2216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2228"/>
            <end position="2247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2259"/>
            <end position="2278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2285"/>
            <end position="2305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2311"/>
            <end position="2339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2360"/>
            <end position="2382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2478"/>
            <end position="2501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1127"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1832"/>
            <end position="1853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="2646"/>
            <end position="2667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="2602"/>
            <end position="2636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1127"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1151"/>
            <end position="1160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2667" mass="305056" checksum="1428D1BDD9709AB5" modified="2018-12-05" version="1">MSDSMSSFLHIGDIVSLYAEGTVNGFISTLGLVDDRCVVEPAAGDLDNPPKKFRDCLFKVCPMNRYSAQKQFWKAKQAKHEKDKIADVVLLQKLQHASNLEQKQNETENRKVHGDVVKYGSVVQLLHMKSNKYLTVNKRLPALLEKNAMRVTLDATGNEGSWLFIQPFWKLRTNGDNVVVGDKVILNPVNAGQPLHASNYELADHSGCKEVNSVNGNTCWKINLFMMFSDHKDEVLKGGDVVRLFHAEQEKFLTCDEYKSKLHVFLRTTLRQSATSATSSNALWEVEVVHHDPCRGGAGHWNSLYRFKHLATGNYLAAEENPGYKGDSSEVQQVTDTESKRSNKRSMGERIKYKLVAVLQGNDIASLFELDPTTLQKTDSFVPRNSYVRLRHLCTNTWIQSTNVPIDIDEERPIRLMLGTCPTKEDKEAFAIVSVPVMEIRDLDFANDASLMLCNMVEKFNSGFISQNDRRFAIKLLEDLVFFVVDQVNSGQPVLDVVNSKPNRERQKLMREQNILKQIFGIIKAPFKDKGGEGPLLRLEELSDQKNAPYQYMLRLCYRILRHSQEDYRKNQEYIAKQFGVMQSQIGYDILAEDTITALLHNNRKLLEKHITKTEVETFVNLVRRNREPRFLDYLSDLCVSNNVAIPVTQELICKCMLDPKNQDILIKTERRTPKENHHHAEYIEDYGDEDEVWLIWMDKANETQEKSIRALAQEARQGNAHDENVLTYYRYQLKLYARMCLDRQYLAIDEISKQLDVELISLCMMDEVLPYDLRASFCRLMLHAHVDRDPQELVTPVKFARLWTEIPTSITIKDYDSHMDYSRDNKKNKFANTMAFMEDYLNLVLHDDLPFADEEKNKLTYEVVSLARHLIYFGFYSFFELLRLTRTLLGIIDCIPNPALVAPMIHDDGSSKNIRRSIHGVGQMMSTMVLSRKQSIISGPCRPIIGLDGQTRTKDSIDNHDITVMDTKLKILEILQFILNVRLDYRLSFLLSVFKKEFVEVYPMADADAVSAMETAASINLQYIGEQAEAMFGVGKGNSILEVDDEGGRMFLRVLIHLIMHDYPPLVSGALQLLFKHFSQRQEVLHTFKQVQLLISEQDVDNYKLIKSDLDHLRMMVEKSELWVDKKSSSGGEGKKDKGEAAANELPVSQKEKREKSNENYQKVKEILERLNRMCSSGVFKKQQRLLKNMGAHKVMLDLLQVSYDRNDKKMLEIIKFTHLFLQKFCNKNPENQALLHKHLNLFLTPGILEAETVQQIFNNNYLLCTEISETVLQHFIHCLATHGRHVQYLNFLYTIIKAEGKYVKKCQDMIMTELTNAGDDVLVFYNDKASFAAMLELMAESREGVQESSPLRYHISLVELLAACAEGKNVYTEIKCTSLLPLEDVVRVVTHEDCITEVKTAYVNFVNHCYVDTEVEMKEIYTSNHIWNLFENFIVDMARVCSNREKRVQDPVLEKYVLNVVLDTINAFFSSPFSENSTSLQSHHTIVTRLLQSSMRLLDCTWLQQQQKGLVESCIRTLAMTAKSRSISLPMELEAHINMMLNSSAVNSVSRPKPGYKNATLTARPASSSSPWDYKNIIEKLQDIINTLEERLKPLVNAELSVLVDVLHQPELLFLEGTDARMRCESGGFISKLIQHTKALMNSEEKLCIKVLRTLQEMLIRTLDFDERGIALRKVLLQNYLFNNKKALKSDLAELGAGGGEAERDWVAVAAVQCRLDREGGTKLFTDLIMSTKNDKIFQESIQLAICLLEGGNTEIQNSFYKLMMGDNKSEKFFKVLNDRIKNAQMDIKATISVNVGEMSNKAKDEKDLDPAASQSLAFHPGTVAQFGHHPPITQSHNLAPAHQTEQQEVETEMGPSVVIMKPILRFLQLLCENHNQDLQNFLRCQNNKTNYNLVCETLQFLDIMCGSTTGGLGLLGLYINEHNVGLITQTLETLTEYCQGPCQENQTCIVTHECNGIDIITALILNDISPLCRYRMEMVLQLKDNASKLLLALMESRHDSENAERILFNLRPRELVEVIKKAYLQESEHEGEEVSPREVGHNIYILALQLARHNKILSHLLKPPKRTKEEGQEGISSMLNLNNKQFSQMLKSSVPAEIQEEDPLDFYDHHTAQIEIVRDDRSMEQIIFPVHPICEFLTEESKFRVFNTTEQDEQGSKVTHFFEQTSFLHNEMEWQKKLRSMPVLYWFSRRMTLWGTISFNLAVFVNLIIAFFYPYDSGQSAIDSSMLLMLFWCFAGLSILGLFSQRYGLQPLTLAIILRFIYHFGIGPTLILLGTLNLINKIVFVVSFVGNNGTFIMGYKAMVMDMEFLYHLAYVFTSTLGLCVNELFYSILLFDLIYREETLFNVIKSVTRNGRSILLTAVLAIILVYLFSIVGFLYLRDDFIMEVDYLSNSNTASESTVGSCSADRTDCTEETGLLAPSQEEEDNTERACDTLLMCIVTVLNHGLRNGGGVGDVLRRPSKNETLFPARVVYDLLFYFIVIIIVLNLIFGVIIDTFADLRSEKQKKEEILKTTCFICGLERDKFDNKTVSFEEHIKLEHNIWNYLYFIVLLQEKNKTDYTGPESYVSHMIKNKNLDWFPRMQAMSLVVTDGDGEQNEMRNLQDRLAATMKVVTQLTSQLAELKEQMTEQRKRRQRMGFVDVQAGSNASLPPSAAGGNHQIYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK45</accession>
    <name>A0A3B4EK45_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Euchromatic histone-lysine N-methyltransferase 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017570239.1">
        <property type="nucleotide sequence ID" value="XM_017714750.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000035654"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028958.2">
        <property type="protein sequence ID" value="ENSPNAP00000035654.1"/>
        <property type="gene ID" value="ENSPNAG00000025826.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437578"/>
    <dbReference type="CTD" id="402830"/>
    <dbReference type="GeneTree" id="ENSGT00940000156002"/>
    <dbReference type="OrthoDB" id="5792673at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046974">
        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20905">
        <property type="entry name" value="EHMT_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000005">
        <property type="entry name" value="Euchromatic histone-lysine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043550">
        <property type="entry name" value="EHMT1/EHMT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047762">
        <property type="entry name" value="EHMT_CRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46307">
        <property type="entry name" value="G9A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46307:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EHMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21533">
        <property type="entry name" value="EHMT1-2_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="779"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="812"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="845"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="879"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="912"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="945"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="9">
        <location>
            <begin position="1067"/>
            <end position="1130"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="1133"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="651"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1276"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="286"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="652"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="689"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1281"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1297"/>
            <end position="1307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1307" mass="143234" checksum="8EDB2B12070526A1" modified="2018-12-05" version="1">MLCLSFSKADQQRELLGHVTKPGERLHAGGGKRRTICSKLSRSPSMEAIGRKQPAGLAGGTVGKRESMKADGMKDSHKDTEGGDEDCLNRKMEPVKTDAELNGTYESTETGKAQQGLSVQESPTQSQAMENGLSETDPPHGSTVGSNGYILNKHQEDTPATQHRTSWSPVGTSAIGHASKTLPPLVKSQHQGQAPLRTDMSKGPSLAEGRSEKETKNGTTSNTPPPVTIHRARKTMSRPASNHPLKLLNRETKEPSVVKDKSASGAETGLPLQTPPAQKQLPQSQTDATPAAIATPAKPHTVASVLRKKKRKMGTYSLIPKKKTKVLKQRTVLEMFKNISKSPPSPQQPKDTTQVNGERVENDSEEEESEEDSEDEEELAVEERVTAPKEGSRMPSVPQLGEEQESEESAEEEGGEEGNESDLSSESSLKKKLKKKTKGDSAWLRPTRKRKKKLKSKSEGMDVQSRSETQSPGQAPVPAGGKEYTEVPLETLNLKAQDALLNTQNTGVSGTVETAASDMVQELPLCSCRMETPKSREILTLADRKCMATESVDGQLSRCQSAVLKHEMMRPSNLVQLLVLCEDHRTGMIKHQCCPGCGYFCRAGTFMECQPEVNISHRFHRSCASVVKGQSFCPHCGEEVSKAKEVTIAKADTTSTVPPSQGPSTPGTVEGRADTTTGGSSRLSVPGENRADSTFHRPAEAVDAPGTSRSGTGVWTGPPGVPPKETLETVLLALDAEKPKKLRFHPKQLYISAKQGELQKVLLMLVDGIDPNFKMENQSKRTPLHVAAEAGHQEVCHMLVQAGANLDMCDEDQRTPLMEACENNHLDTVCYLLRAGAIVSHKDGEGSSCLHLAAKNGHLSIVEHLLSTGLVDINCQDDGGWTAMIWATEYKHIEIVKQLLSKGADPNIRDKEENICLHWAAFSGSVDITQILLDAKCDLNTVNIHGDSPLHIASRENRLECVTLFLSKGADGNLKNREGDTPMECCSQNSKVWNTLHASRKQRDASRERRSSKEKLLSRDIARGYERVPVPCVNAVDSEPCPDDYKYVPDNCVTSPMNIDKNITHLQYCVCKDDCSSASCMCGQLSLRCWYDKESRLLPEFCCEEPPLIFECNHACSCWRTCKNRVVQNGLRIRLQLFRTLKMGWGVRTLQDIPQGTFVCEYVGEIISDAEADVRENDSYLFSLDSKVGDMYCIDARFYGNISRFINHHCEPNLFPCRVFTSHQDLRFPHIAFFASKNISAGDELGFDYGDHFWDVKGKLFSCQCGSSQCKNSAAAIAQRQADSTPGEQQASALPDTSSSTTPPSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK58</accession>
    <name>A0A3B4EK58_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAM196B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017572384.2">
        <property type="nucleotide sequence ID" value="XM_017716895.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK58"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036275"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030593.2">
        <property type="protein sequence ID" value="ENSPNAP00000036275.2"/>
        <property type="gene ID" value="ENSPNAG00000026965.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438824"/>
    <dbReference type="CTD" id="100131897"/>
    <dbReference type="GeneTree" id="ENSGT01090000260190"/>
    <dbReference type="OMA" id="LSHTMPK"/>
    <dbReference type="OrthoDB" id="8679980at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029337">
        <property type="entry name" value="INSYN2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28682">
        <property type="entry name" value="INHIBITORY SYNAPTIC FACTOR 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28682:SF2">
        <property type="entry name" value="PROTEIN INSYN2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15265">
        <property type="entry name" value="FAM196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036275.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="65338" checksum="C97157D51F117383" modified="2024-11-27" version="2">MGRRAAETTNPVPVLGVPLAGGRAVLSQKWGPLCSVGVQTSPGLRSLPSLKRRNQPIGTIHGVSTETMSLDRVRPAIKGAFQNCISNVSQDDMSQDGGIYCQIKSVQPNPNQPGCRGNLKRNPRYANGSIVAPELVGGVSSEGAETAEAAGQNRDKTQEPKRGHSLRGEKARPVGHCGIVSSYATPPRPCRMMTSPRLCGTCGRRQSQAPPCMIHTCRKRSANQIRASMTLPMPPKKNLPRLQKQHSTETNPTDTSHTDQSMHKESSSLSTQSQKRDTTTHETQTQTNDVARQSVHDKICKNDSLTSQSFKRQSKQKSSQHTQTQNVQKGAPITKIQPTESAHEKQYTLKDIQPREDCVNKQTPPAPEFAHPSVYVTPKPPPPVLSIGTESKTTSILPPNNSSQSKPTELTPKTASPKTQNRSEIKPIPKTPPTYQEPTVENDPETKPCCVDPIQPPQKDTTTPDVSQCNGVPTALQGLLQDIEENLLSNQEKIKVLLNVIQDLEKSKAMSEGRCSYRTGQDINNCSTCQKTACIIYSVEHDFRLQEGRFQSVFEALDVEYDVPTPIPKPIAPSRPRTKNRVKKLRKKCFWWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKJ6</accession>
    <name>A0A3B4EKJ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CTCK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560429.1">
        <property type="nucleotide sequence ID" value="XM_017704940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EKJ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036415"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030926.2">
        <property type="protein sequence ID" value="ENSPNAP00000036415.1"/>
        <property type="gene ID" value="ENSPNAG00000027210.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431645"/>
    <dbReference type="CTD" id="100002201"/>
    <dbReference type="GeneTree" id="ENSGT00940000154209"/>
    <dbReference type="OMA" id="FFCVIAE"/>
    <dbReference type="OrthoDB" id="10061784at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038098">
        <property type="term" value="P:sequestering of BMP from receptor via BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004133">
        <property type="entry name" value="DAN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15283">
        <property type="entry name" value="GREMLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15283:SF2">
        <property type="entry name" value="GREMLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03045">
        <property type="entry name" value="DAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017254485" description="CTCK domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="8">
        <location>
            <begin position="95"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="200" mass="22933" checksum="0926F171EFB6159C" modified="2018-12-05" version="1">MRCCNYRMFWKLALPAILVWTLCASTGTKKPRPRGSIPSPYKLKGNELSSSPSILLSSLSQQPLTRRQKGKQEVLSSSREALVVTERKYLKSDWCKTQPLRQTVSLDGCLSRTVVNRFCYGQCNSFYIPRHLTSRSPARHSRKTDDHDTSFQSCAFCRPHRITTVTVRLHCPGLQPPYRHRKVQRVKQCRCVSVSVNDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EL77</accession>
    <name>A0A3B4EL77_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRIM25</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EL77"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036533"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031245.2">
        <property type="protein sequence ID" value="ENSPNAP00000036533.1"/>
        <property type="gene ID" value="ENSPNAG00000027470.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164979"/>
    <dbReference type="OMA" id="VCYGSMP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19776">
        <property type="entry name" value="Bbox2_TRIM25_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF77">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="396"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="225"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="589" mass="66142" checksum="0FBECF6B081E3DAD" modified="2018-12-05" version="1">MATALLNLEEDLTCSICLCPFDSPVSLSCGHSFCQQCLEAAWKDAGPVSCPQCRALYSVRPVLKRNTVLSAVVDTIKSASDAGASPSSGEREEPKPVLCDTCMQAKAESTCMTCAASFCSEHVRPHRENPVYRAHRLVEPPLADLQNRLCAEHAKLLEFYCAPHERSICSGCLQEQHRTCSFYTVEEQREKKESELKEKLDSLDGKIEKNQMVISQMREQQAKLKDVAAVRKRALEAEYQQLRDMLQRDEKEAMMALEKDLESGQSKLSMLMKKFNQNIEKMSGTKDEINRLLVQSHSLDFLETNMDLPSVVTFDPYAPRINLDSKCIIAHHACIVALKEQISKILQQPVENRLSLLKPGGCIHVIYTVYTIMIILNNNHFSFFPGAKKFPNKGMAPPFFRRMDGGMNFTSDATILMFDQRTAHRRVVLSENNTKASVSDEPSAYPDLPTRFTVCSQVLCSKGFSQGCHYWEVQMSSNNFCGIGLAYGSIDRKGPSSRLGRNAQSWCIEWFNVKLSTWHNNRETVLNNPNPSRVGVLLDCSGGTATFYNVVADRAHAFHTFVFPFSEPVYPAFWIFSSGSSVNLCKLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ERE7</accession>
    <name>A0A3B4ERE7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">protein-glutamine gamma-glutamyltransferase</fullName>
            <ecNumber evidence="7">2.3.2.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.13" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017553268.2">
        <property type="nucleotide sequence ID" value="XM_017697779.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ERE7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037731"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041188.2">
        <property type="protein sequence ID" value="ENSPNAP00000037731.2"/>
        <property type="gene ID" value="ENSPNAG00000006893.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427553"/>
    <dbReference type="CTD" id="565984"/>
    <dbReference type="GeneTree" id="ENSGT01050000244866"/>
    <dbReference type="OMA" id="DTFWETH"/>
    <dbReference type="OrthoDB" id="437511at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.260.10:FF:000001">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF80">
        <property type="entry name" value="TRANSGLUTAMINASE 5,-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00927">
        <property type="entry name" value="Transglut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00868">
        <property type="entry name" value="Transglut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000459">
        <property type="entry name" value="TGM_EBP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
        <property type="entry name" value="Transglutaminase, two C-terminal domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="9">
        <location>
            <begin position="260"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037731.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="675" mass="75473" checksum="BF1EE40D801F5AD9" modified="2024-11-27" version="2">MDALRVQSVNLEHAQNQRQHNTEGLNSASLVVRRGQPFKLTVAFNRRYIPNRDGLILRVELDRLYNDIPITFSRDPSQWSAYFQQGSLDSTKASLCVCSPANAPVGVYQLQMMSPSGPYNFRVGQFTLLCNPWCSADSVYIADESQREEYVKNDFGLLYQGTAKNVIFRPWSFDQYENGILDICMMLLQVSPEHKRNWRTDNSNRSDPIYISRVVSAMINSNDDCGVLQGNWSEDYSGGVNPSDWNGSGEILRQWAQSRCSPVKYGQCWVFAGVMCTVMRVLGIPTRVVTNFDSAHDTNGNLVVEEFYTETGEKLPRGQDSIWNFHVWVECWMKRADLRAGFDGWQVLDPTPQERSEGVYRCGPAPVKAIREKRMDMPFDIPFIYAEVNADVYVCIVQQGKVLSCSIDTDKVGSLICTKSLGSNRPQDITSTYKYTTAIQPVTSNSTMAAPTLANTSRSPDGLSVKLRFLKAPVIGGNISFSVTVTNLESTWKTVRQHVNAQAKSYNRSPSDTFWEAHNTIQLAPHESKVLEHQISYRQYAMLGNRQLVNLAVVVMDVASQKRVLASEEFNLTSPTISIQIEDEGSVVAQKVQVAMVTFTNPFSEPVSGVLTMAGAGLLEDKVFFNIALQVGESMQTAVKFTPKMAGVKMLHANLRLNNNPAVIRGFRTITVRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IHB6</accession>
    <name>A0AAR2IHB6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017578615.1">
        <property type="nucleotide sequence ID" value="XM_017723126.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IHB6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046158.1">
        <property type="protein sequence ID" value="ENSPNAP00000038635.1"/>
        <property type="gene ID" value="ENSPNAG00000034621.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108442869"/>
    <dbReference type="GeneTree" id="ENSGT00940000165302"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15734">
        <property type="entry name" value="FYVE_ZFYV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042427">
        <property type="entry name" value="ZFYV1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46624">
        <property type="entry name" value="AGAP002036-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46624:SF2">
        <property type="entry name" value="SI:CH211-11N16.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="5">
        <location>
            <begin position="477"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="5">
        <location>
            <begin position="592"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="654" mass="72588" checksum="297964CC7CCD4219" modified="2024-11-27" version="1">MSEMLLKEMEAVSSLPLLPKKEDPGSERRSFLLVDENETLQEKDEAQFLQKLGCSPGQGVKVLSIFGNTGDGKSHTLNHVLFDGEEVFVTSPSPTSCTIGVWAAYDQHLGLVVLDTEGLLGASTQMNQRMRLLLKVLAVSDIAVYRTRAERLHNDLFQFLGTASAAYLKHFTPQLRALSSRCGLDVPLSNLGPAVVVFQETSRTQLLGHDGTAPADVLLQKRFHDLGQSTEAFSSVQYVGTQTVTPPTDYSQFLETIRQQVKNTASRAPRQPAIVFSALQALSERFCGEISDDKVSMYSFFPDEYFTCPSVCLSCNVRCKNGMNHQKDRVPHMADGLCQYAHQYSNKVLICKRCYEGGREVIVVPKTSASTDNPWFGLAKYAWSGYVLECPNCGIIYRSRQYWMGNQDPESGVVRPEVKHVWQGSEAFQTDHQNAAQRVLDGVNYVIQSMAEYSAGPTKAVTAWITDQVAPPYWRPNAEIIVCHGCNREFEEAERKHHCRSCGEGFCQACSTRSMPVPERGWGSSPVRVCEACHRQGGALQTVQVSQTEPRTLVARRVTEVAQSTLNMVSNAVDYPLCFVKGVARPDYWVPDHEITECHQCSKGFTSSIPKHHCRACGQGVCGNCSTQNRPVPSRGWDHPVRICDACVTHLDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJ12</accession>
    <name>A0AAR2IJ12_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type phospholipid transporter</fullName>
            <ecNumber evidence="2">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ABCA7</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053005.1">
        <property type="protein sequence ID" value="ENSPNAP00000039690.1"/>
        <property type="gene ID" value="ENSPNAG00000015779.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154658"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000232">
        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000264">
        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF234">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="636"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="678"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="713"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="742"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="818"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1338"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1637"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1692"/>
            <end position="1711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1717"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1748"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1835"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="897"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1894"/>
            <end position="2126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1263"/>
            <end position="1292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2229" mass="249020" checksum="F5B05B0D83277C32" modified="2024-11-27" version="1">MGLLTQLRLLLWKNFTLRRRQKLRLVIELVWPLFLFFILVWVRSTNQPTYKGQCHYPNKPMPSAGVLPWLQGMVCNIQNPCVSQPTPGETPGQVNNFNNSILAKALIEVQSLLADSTFLSNLKLLSGDINQWASILQNSNPANGRSISLRSVLRDNETFSEYLRSNLSVPTQTAQRLTSMRVRLNQLPALSAETNLRTALCSSDSVDRYLEFNTTIQRQEFLNITCSLTPNQLLQTREVLIRNLDNRKLATALPLALNLNPAETAVLLAKTSTHAAPVADQVLKLQTSTMLSAVNVLDFRSNPFGSLTLLMCGGDSGFNSTKSSNSSSGSNQGFRLPTQSTNALSLNSTHGNSSVFCQNLVDVLKGTPSLRLFWTTLRPLLQGRVLYTPDTPFTRRVIAQSNSTFDALSKVEVLAETWLENEDEIWNFFKNSNQINSLRTILQNPLFVAALDQQLNRTSLSSALIANFLFNGDEKDRPVGMPSFDWRNIFNATSEAARFLKEVMGCFDLDKFEAISSESLLVQRAMKLLDEERFWVGVVFTDLDSPSLVRYKLRMDIEDTERTDKIKDRSWRPSARDNPYNDLRYVRGGFVYLQDMLDRGIIRTQTGVQQTLGVYTQQMPYPCYVDDAFVSSIATILPMFLVLAYMYMVCQTIKGLVLEKEMRLKEVLRVVGVRNGPLWFAWFTENMVLLTIPCALISVMVKYGKVLKYSDPTVIFVFLMVFCMATVMQCFFISVFFSRANLAAACGGLIYFLLYLPHVLCYAWRDVITFRAKLATSLLSCVAFGYGCENFARYEEQGVGIQWKNIGISSQEDDPYSFIVSIIMMLIDTLLYWILTWYIENVFPGQYGIPKPWYFPFTSSYWCGTPVGSYNEAEPLDALEGQNGYLEKASAGLKPGVSIRNLVKVYKTGKKLAVDKLSLDFYQDQITSFLGHNGAGKTTTMSILTGLFAPTSGTAYVNGFDIRTDMDSIRKTLGMCPQHNVLFNDLTVEEHIYFYARLKGCSSEEVKQEIDQMIKDVGLPHKRKELAKNLSGGMQRKLSVAIAFVGGSNVVILDEPTAGVDPYARRGIWELLLKYKKGRTIILSTHHMDEADILGDRIAIISHGRVCCCGSSLFLKKSYGSGYYLTLVRTNQDKFNNLDKPQSDGTSLDEGIGSTSICSMEPTDVADVSGLVRRHVPEAELLEIIGQELVYILPYTGSKDGTFASLFKELDLERERLGISSYGISDTTLEEIFLKVAEETGVDVETTDKKNVLERDWKRSSRESRTRSISSAMQQRESEASKHGEKKGRFSNRVEDGERSVCVNGKGSCVITGWPLIRHQFLALFIKRFHHARRSRKGIIAQVVLPAFFVCLSLIFSLIVPPFVEYPSLELQPWMYGKPQNTFYSADEPAVAEVVSVTRSLLNEPGFGTRCMESNPIPKYPCVPAGATDWSTPAVDPSILEIFTIGNWSMQNPSPNCQCSTPQRNIMLPDCPPGAGGLPPPQRTQNTTDTLHDLSGRNMTDFLLKTYAKSGKIRYGGISVGGVNSQVRMNESEITAALLDLKALFNSSQNDVIDKVFQDLETLLIELGTKDNVKVWFYNQGWHAIASFLNIATNAILRGNLPARQQPKLYGISAFNHPLNLTKQQLSAFLASSSTDVVVSICVIFAMSFIPASFVLFLIQERVSKAKHLQFVSGVNPAGYWVANFTWDMCNYIVPCIIVIVIFLCFQQKAYVSPPNLPALILLLVLYGWSITPMMYPASFIFSVPSTAYVVLTCINLFIGINGSVATFVMELFDDPNVTRINDIVKQVLLIFPHFCLGRGLIDMAKNQAMATLFSSMGEDRFKDPLGWDMVGKNLFAMAIEGVVMFGFTLLIQYKFCWKPRLVTGLPVASVEEDEDVARERERVHRGGAQNDLLQICNLTKVYSGKKVPAVDRICVGVPAAECFGLLGINGAGKTTTFKMLTGDIPASGGEAFLNGYSIRTEMSQVHQNMGYCPQFDAIDDLLTGQEHLEFYARLRGVPEREVTTVAQWGIQKLGLVKYSSKSAGTYSGGNKRKLSTAMALIGCPPLVFLDEPTTGMDPKARRFLWDCILSVIKEGRSVILTSHSMEECEALCTRMAIMVNGQFKCLGSIQHLKSRFGDGYTVIVRVQDGPADLGSVEDFVCRTFPGSVLKEKHHNTLQYQIPHTEGALSHIFSEFSKHQQALGVEDYSVSQTTLDQVFVNFARLQHDTEDGVYENPVDLSDEIPMQRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IP10</accession>
    <name>A0AAR2IP10_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037392289.1">
        <property type="nucleotide sequence ID" value="XM_037536392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IP10"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058807.1">
        <property type="protein sequence ID" value="ENSPNAP00000039744.1"/>
        <property type="gene ID" value="ENSPNAG00000032848.1"/>
    </dbReference>
    <dbReference type="GeneID" id="119262929"/>
    <dbReference type="GeneTree" id="ENSGT00530000065055"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031833">
        <property type="entry name" value="DUF4748"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15932">
        <property type="entry name" value="DUF4748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="18729" checksum="7E05FD2B7C3EA959" modified="2024-11-27" version="1">MSAPCWNLLRSAVGGLCYLPTRRLCLRAGHVQIVTNHRSQPSVLHCRALHCSRYPLLNSTKTESQRAAEAPKAPETTSGQKKAEDDEVEEEYGPEYIPRRKAKNPMTIIGYAWIIGLPTGIIGFILAKKQVDKNRLKQLRIRQRMKKANEGEYERERYKPAAGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZ07</accession>
    <name>A0AAR2IZ07_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZ07"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081285.1">
        <property type="protein sequence ID" value="ENSPNAP00000043279.1"/>
        <property type="gene ID" value="ENSPNAG00000022018.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167069"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF23">
        <property type="entry name" value="MITOCHONDRIAL CARNITINE_ACYLCARNITINE CARRIER PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="14"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="114"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="213"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="306" mass="33049" checksum="72FE8FC4B77971F1" modified="2024-11-27" version="1">ANICLFPRPAFIRVSPLKNFVAGGVGGACLLFAGHPLDTIKVRLQTQPKASCSQYVLYTGTYDCFRKTVSREGVLGLYKGMGAPMATVAPMMAISFFGFGLGKQLLQSDPAAPHTHSQIYLAGMLAGVFTTVIVAPAERIKCLLQVQSTTGQLKYAGPVNCAVRLYREQGIRSVYKGTVLTLIRDVPSSGLYFLTYEYLKSVLTPEGQSVHELSTPRILLAGGTAGILNWVIALPPDVLKSNFQTAADGHYKGLVDVLRTLIREEGAKALYKGLSAVMLRAFPANAACFLGFELALKSLNWLAPNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J4C0</accession>
    <name>A0AAR2J4C0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">37 kDa laminin receptor precursor</fullName>
            <shortName evidence="13">37LRP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">37/67 kDa laminin receptor</fullName>
            <shortName evidence="13">LRP/LR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">67 kDa laminin receptor</fullName>
            <shortName evidence="13">67LR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Laminin receptor 1</fullName>
            <shortName evidence="13">LamR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Laminin-binding protein precursor p40</fullName>
            <shortName evidence="13">LBP/p40</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RPSA</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer (37LRP) and homodimer (67LR). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21. Interacts with several laminins including at least LAMB1. Interacts with MDK. The mature dimeric form interacts with PPP1R16B (via its fourth ankyrin repeat). Interacts with PPP1CA only in the presence of PPP1R16B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <text evidence="13">67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus.</text>
    </comment>
    <comment type="PTM">
        <text evidence="13">Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.</text>
    </comment>
    <comment type="PTM">
        <text evidence="13">Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="13">This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541476.1">
        <property type="nucleotide sequence ID" value="XM_017685987.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J4C0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041869.1">
        <property type="protein sequence ID" value="ENSPNAP00000045089.1"/>
        <property type="gene ID" value="ENSPNAG00000020586.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413467"/>
    <dbReference type="CTD" id="3921"/>
    <dbReference type="GeneTree" id="ENSGT00950000183099"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005055">
        <property type="term" value="F:laminin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000012">
        <property type="entry name" value="40S ribosomal protein SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03016">
        <property type="entry name" value="Ribosomal_S2_laminin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032281">
        <property type="entry name" value="Ribosomal_uS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027504">
        <property type="entry name" value="Ribosomal_uS2_vert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16122">
        <property type="entry name" value="40S_SA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 13 14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 13 14" id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein uS2 C-terminal" evidence="16">
        <location>
            <begin position="202"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="[DE]-W-[ST] 1" evidence="13">
        <location>
            <begin position="230"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="[DE]-W-[ST] 2" evidence="13">
        <location>
            <begin position="245"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="[DE]-W-[ST] 3" evidence="13">
        <location>
            <begin position="279"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="[DE]-W-[ST] 4" evidence="13">
        <location>
            <begin position="288"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="[DE]-W-[ST] 5" evidence="13">
        <location>
            <begin position="306"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Laminin-binding" evidence="13">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Laminin-binding" evidence="13">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
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        <location>
            <begin position="278"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by ST3; site 1" evidence="13">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by ST3; site 2" evidence="13">
        <location>
            <begin position="133"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylserine" evidence="13">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="308" mass="33975" checksum="5EFFE9BB102C4939" modified="2024-11-27" version="1">MSGGLDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYVYKRKSDGIYIINLKKTWEKLLLAARAIVAIENPADVCVISSRNTGQRAVLKFAAATGATTFAGRFTPGTFTNQIQAAFREPRLLIVTDPRADHQPLTEASYVNIPTIALCNTDSPLRYVDIAIPCNNKGPHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVGKEEFQGEWTAPVADFAQPEVADWSEGVQVPSVPIQQFPASIEAKPAPAATEVYADDWSAQPATEDWSAAPTAQAGDWGGSTADWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JE83</accession>
    <name>A0AAR2JE83_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Paraneoplastic antigen Ma1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JE83"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077951.1">
        <property type="protein sequence ID" value="ENSPNAP00000048166.1"/>
        <property type="gene ID" value="ENSPNAG00000032851.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234522"/>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
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    </dbReference>
    <dbReference type="InterPro" id="IPR048271">
        <property type="entry name" value="PNMA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Paraneoplastic antigen Ma-like C-terminal" evidence="2">
        <location>
            <begin position="151"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20846"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="38082" checksum="8C0059BBB8E97DE3" modified="2024-11-27" version="1">MAQLRHWCKGEGINPDNALLVKDIPDDTEVSFIEETLESVKALGRVRVRGRMYDPQLESLTVLCECREKVNVKTPPPDVLSSNTDRPWRLVGASAEQTTERNKPDENPVLSQAELAQTCAPDAIIRAVGELMEKQGRFIGESSAYRRLRTFSGVVPTPPGEEQLDSWLEQARLMVEASERPDKEKRMRILQSLKGAASEIVQAVRFNDPDATPQEYIDALENAFDTPETGEELYFAFRHLCQKPREKLSEFLRRIEKALNRVVQRGGLPASAANGARLEQLIKGATTSDLMLLNLRLRERKNSPPNFLQLLNEIRSEEGYEASRHKLSSSGEGDFPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JFY1</accession>
    <name>A0AAR2JFY1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transducer of regulated CREB activity middle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037391079.1">
        <property type="nucleotide sequence ID" value="XM_037535182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JFY1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078316.1">
        <property type="protein sequence ID" value="ENSPNAP00000049119.1"/>
        <property type="gene ID" value="ENSPNAG00000030670.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426025"/>
    <dbReference type="CTD" id="200186"/>
    <dbReference type="GeneTree" id="ENSGT00990000204953"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008140">
        <property type="term" value="F:cAMP response element binding protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024786">
        <property type="entry name" value="TORC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024784">
        <property type="entry name" value="TORC_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589:SF6">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12885">
        <property type="entry name" value="TORC_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transducer of regulated CREB activity middle" evidence="2">
        <location>
            <begin position="6"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="423"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="691" mass="76318" checksum="2A44BF6CE004C88D" modified="2024-11-27" version="1">MTTLSRTNSDSALHTSVRVTYFRDSNPAQVQTACSRQSGTPLFRHPVPLIEETVQEEGKPPKSQKETSVLSGCGTIVSCVFPSPDLSCSLPNVPSLSTSGSLPDLSSLHLPSTVTEVIDPEPQSHTSLLSSPTSMHHLPSTAAHSAIMTDHEFPLPSLSSSPQASTSNSSRPSSLSNPNLQATLSSHSLVDSFSSTSLSLSNSSLHSSVSSQSLRSSLSSSSLSNQSAQSADSHCSYSSGIGGSFSSLSCSPHASGQGVVPRNTSSWKRTQLNPLIVPSGGESLWQQPKQLSPNVSSTFSSIAQGVPLDTTKVQREAKPSIYHYSHLPSTGHPMAYQSLQHHQPEGWDQSRQDTRKQCQQQVLQPSSSQLKSQPQYSQHSAHGKAHLNPHPCISQQQFTEQIQDLHHQYQQVQQQSLHQQYQPSQQYLQQQPQHCQSQHNTQPLQHQQQYQDRNQGWNPQLQLSHHNQYQQQCQYQSQPEGPPGTFQKQQPCPPPTVMASRDEDAGCWQKRVNQHNQHIPNIGIQGPLSACMHIKELPKVQSKMPVHDLRRERTTQYPKGIHSHRDHLQLSETASAPSLNKEFGLHNKSYVGLHLTPSQTEALSQKLGHLHKEPAEAPRISDSKCQDVEDKDVPAVENLWHPETQSQNKEGLHNFSSTSTEVPSSWLDDDELPDLPDSIPEFDLEHFALED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JII1</accession>
    <name>A0AAR2JII1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RABAC1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561562.1">
        <property type="nucleotide sequence ID" value="XM_017706073.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JII1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065923.1">
        <property type="protein sequence ID" value="ENSPNAP00000049999.1"/>
        <property type="gene ID" value="ENSPNAG00000032207.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108432321"/>
    <dbReference type="CTD" id="10567"/>
    <dbReference type="GeneTree" id="ENSGT00390000010549"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF0">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="189" mass="20853" checksum="FCD0CDC42DFDBC11" modified="2024-11-27" version="1">MESKMSDPFSSDSEALPAAGIMARLLPKGLSGTVLKDWMDRRRKSIRPWAGFVDQRKFSKPKNFGDLCQRVVRNLDTYHSNYTFIFLGLILYCIISSPMLLIALAVFAGAFYIIHLKSLESKLVVFGRELTQGHQLGLAGAVSLPVFWLAGAGAAVFWVLGATLAVIGTHAAFRELESSDMEELLMEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JR07</accession>
    <name>A0AAR2JR07_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JR07"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041982.1">
        <property type="protein sequence ID" value="ENSPNAP00000052624.1"/>
        <property type="gene ID" value="ENSPNAG00000021659.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015789"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000003">
        <property type="entry name" value="Eukaryotic translation initiation factor 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="166" mass="18433" checksum="C07055678F99D93B" modified="2024-11-27" version="1">FLFWLNNHPFADATKGDDRLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLTDVSGFSCFSFALGLRLNSGLSETAVGLNLGLKLAMSLVLLALSLPLNRLNWPKRSSSRSTVSRSVADPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JY09</accession>
    <name>A0AAR2JY09_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Coiled-coil domain containing 149</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC149 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564526.1">
        <property type="nucleotide sequence ID" value="XM_017709037.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JY09"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042337.1">
        <property type="protein sequence ID" value="ENSPNAP00000056943.1"/>
        <property type="gene ID" value="ENSPNAG00000035742.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108434121"/>
    <dbReference type="CTD" id="436983"/>
    <dbReference type="GeneTree" id="ENSGT00390000015958"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019179">
        <property type="entry name" value="CC149"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21682">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21682:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09789">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="422"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="554"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="25"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="65289" checksum="7376E1DF6BBFE93B" modified="2024-11-27" version="1">MNPSRRSESDWQGLVNEFLVCKRKLESKKEALLILSKELDTCQQERDQFKLMANQLRERHQGLKKKYRELIDGDLTLPPEKRSQVNLSQLLSDAQKKNRKLSDEVKELNQRLAEVQGDNKLLRMTIAKQRLGDEEVGVRYFPAHEREDLVHQLERAREQNEELEHSLKAAADELQDVRAERKVYQEKAHRLNLEINHILGCHDNRILDVDALCLENRYLHERFVQIQEEVNLLKTNLMKYKSALESKKNCKVYGKANSSALTGVLSAKQVQELLLSEENGCSLPVTPQSISDLKSLATALLETIHEKNMVIQHQRQTNKILGNRIAELEGKLKTLEVSGLWSLPGITYNVSVGLGRGKDVIILSDQQPDQSTMDHLAPLRQASMGDEEVVTGEEVTEDTALCADPGHSETTKMFPRPEHHSAAFSKNTNTAGSSFQTGTMSSACPSDDLSSEHSYPACFDSEKTVCAMERVSDKLQSLDSDGDLATHGDRNSKNAQVRFEGPAEEAGEDAEVLGFEQHDIQGQSESTKVMASESQGCVNYTILSDPTTAEYIESTHCTKEETDQNLSLPDTQAPDQEVNPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1B1</accession>
    <name>A0AAR2K1B1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Paraneoplastic antigen Ma1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1B1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047660.1">
        <property type="protein sequence ID" value="ENSPNAP00000058103.1"/>
        <property type="gene ID" value="ENSPNAG00000031084.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234522"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048271">
        <property type="entry name" value="PNMA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF51">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN MA1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20846">
        <property type="entry name" value="PNMA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Paraneoplastic antigen Ma-like C-terminal" evidence="1">
        <location>
            <begin position="153"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37870" checksum="47EB7B91BE5B2614" modified="2024-11-27" version="1">MSMAQLHHWCKGEGISPDNALLVKDVPDDTEVSFIEETLESVKALGRVRVRGRMYDPQLESLTVLCECREKVNVKTLPPDVLPSNTDRPWRLVGASAEQTTERNKPDENPVLSQAELAQTCAPDAIIRAVGELMEKQGRFIGESSAYRRLRTFSGVVPTPPGEEQLDSWLEQARLMVETSERPDKEKRMRILESLKGPASEIAQAVHFNDPDATPQEYIDALENAFDTPETGEELYFAFRHLCQKPREKLSEFLKRIEKALNRVVQRGGLPASAANGARLEQLIKGATTSDLMLLNLRLRERKNSPPNFLQLLNEIRSEEGYEASLASYACVWTTGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1B9</accession>
    <name>A0AAR2K1B9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MOK</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1B9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085356.1">
        <property type="protein sequence ID" value="ENSPNAP00000056249.1"/>
        <property type="gene ID" value="ENSPNAG00000002684.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437009"/>
    <dbReference type="GeneTree" id="ENSGT00940000159582"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07831">
        <property type="entry name" value="STKc_MOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000271">
        <property type="entry name" value="MAPK/MAK/MRK overlapping kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000773">
        <property type="entry name" value="MOK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="405"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="439" mass="50176" checksum="EBD4F88DE6409254" modified="2024-11-27" version="1">MHPTDNDKKRWEPGQALSNSLSKKGYKIIKKIGEGTFSEVVKVQNLKDGKYYACKKMKQSINSLEQAHNLREVQAMKRLSPHANILQLHELIFDQDMRTLSLICELMEMNIYELIRGRQNLLTESKVKHYMYQLCKALDHMHSNGIFHRDVKPENILIKHDVLKLADFGSCRSVYSKPPHTEYISTRWYRAPECLLTDGYYSLKMDMWSAGCVFFEIMSLSPLFPGSNEVDQLSKIHDVLGTPESAVLQKLKQSRAMHFDFLPRKGCGLSQLIPQCSAPSLSLLYQMLAYDPDERISARVALQHVCFRELRMAERRAVGLHRAMGSVEEGSATPSSVEHLWRIARQGRRQQLKYVGDPLSRRMPHYPVELPKLNVVVPAPKVPYPMPSLPALAIPHRGSLPAITSKKCHSRSTKPREDPHRSALKSYYMPPLERKGGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K2Y8</accession>
    <name>A0AAR2K2Y8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Baculoviral IAP repeat containing 5a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551582.1">
        <property type="nucleotide sequence ID" value="XM_017696093.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K2Y8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068414.1">
        <property type="protein sequence ID" value="ENSPNAP00000056852.1"/>
        <property type="gene ID" value="ENSPNAG00000031971.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426523"/>
    <dbReference type="CTD" id="373110"/>
    <dbReference type="GeneTree" id="ENSGT00510000047537"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000009">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF3">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="16309" checksum="DFF91C809F486832" modified="2024-11-27" version="1">MDLANDGYTKMYFYENRLLTFVGWPFEEGCGCTPENMAKAGFIHTPSGNSPDVAQCFFCLKELEGWEPEDDPKKEHKAHSPTCNFITMKKSVEDLTVEEFLRLEKERQKFGIKKTCQQAIVKFEEAVKLKRGKIIQTAMGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K3U6</accession>
    <name>A0AAR2K3U6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="domain">
        <text evidence="7">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K3U6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065792.1">
        <property type="protein sequence ID" value="ENSPNAP00000056791.1"/>
        <property type="gene ID" value="ENSPNAG00000008092.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155988"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050819">
        <property type="term" value="P:negative regulation of coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000005">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000022">
        <property type="entry name" value="Annexin A5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002392">
        <property type="entry name" value="ANX5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF26">
        <property type="entry name" value="ANNEXIN A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00201">
        <property type="entry name" value="ANNEXINV"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 7" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 7" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="574" mass="63507" checksum="2A42A8315CCD7917" modified="2024-11-27" version="1">MKANEEETTCFFLFLSQAGRGTVKAQSGFNANNDAETLYKAMKGLGTNEATILQLLTARSNAQRQQIKAAFKTLHGKDLVSELKSELGGKLETLIVAMMATPVMFDVTSLRNAIKGAGTNEKVLIEILASRTQNEIREIIKTYKQEYDHNLESDVSGEVGGHFKEMLVILLQANRQQGVQEGTIQSDAQALFNAGEKKFGTDEDQFITILGNRSAEHLRRVFDAYMKLSGHQIEESIQRETSGGLRDVLLAVVKCARSVPAYLAECLYNSLKGAGTDDATLIRIMVSRSEIDLLDIRAEFRKSFATSLHNMIKNDTTGDYCKTLLLLCGGTDEATILQLLTARSNAQRQQIKAAYKTLHGKDLVSDLKSELGGKFETLIVAMMATPVMFDVTSLRNAIKGAGTDEKVLVEILASRTQNEIGEIIKTYKKEYDHDLEQDVSGDTSGHFKRMLVILLQVMLCRMFDAYMKLSGYQIEESIQRETSGGLRDVLLAVGKTRIHTQLSILKYTSLTLSGAGTDDATLIRIMVSRSEIDLLDIRAEFRKSFATSLHNMIKNDSSGDYRKTLLLLCGGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K7G3</accession>
    <name>A0AAR2K7G3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K7G3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069109.1">
        <property type="protein sequence ID" value="ENSPNAP00000058031.1"/>
        <property type="gene ID" value="ENSPNAG00000031526.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163919"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13759">
        <property type="entry name" value="TGF_beta_NODAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000026">
        <property type="entry name" value="Nodal homolog 3-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF159">
        <property type="entry name" value="NODAL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043994974" description="TGF-beta family profile domain-containing protein" evidence="12">
        <location>
            <begin position="22"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="13">
        <location>
            <begin position="298"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="195"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="430" mass="49100" checksum="54983EEF8803E893" modified="2024-11-27" version="1">RETLNLTALLLFLLRCLGCMGIQRKTKPRSGLSKATSEGYSVAAHHPYRYPLYMMQLYRDYKTVDAEKSSTARDARPSLHQSDSVLSLVAKDCQQIGERWTVTFDLSSLSSSEEIQLCELRFRVPAFAASKRAVVDLYHSRQDDCEPDVEHCREEPIFLGTIKPTAVDTTSSSSWRVFNVTALLTYWLRRESSEEPSQTDRFPHGEYSTSEQEEEAEVGVEDAVEGSGGDAVEPVPVKHRSVWTRKIRHPTANRVMVVVFSKSTQARGQVRAPTLLRAVEHSKYVVLQRPGDVTQNRRHKRNRLEMDHFRPVLGEGVTGSPAVSAQRPLCRRVDMWVDFDQIGWNEWIVHPKRYNAFRCEGECPIPLDESFKPTNHAYMQSLLKLYHPERVTCPSCVPTRLSSLSMLYYEGDDVVLRHHEDMVVEECGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KEE9</accession>
    <name>A0AAR2KEE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Discs, large homolog 5b (Drosophila), tandem duplicate 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSPNAT00000056652.1">
        <property type="protein sequence ID" value="ENSPNAP00000060849.1"/>
        <property type="gene ID" value="ENSPNAG00000022040.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155303"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035331">
        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06764">
        <property type="entry name" value="PDZ1_DLG5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06765">
        <property type="entry name" value="PDZ2_DLG5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06767">
        <property type="entry name" value="PDZ3_DLG5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06766">
        <property type="entry name" value="PDZ4_DLG5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11860">
        <property type="entry name" value="SH3_DLG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000130">
        <property type="entry name" value="Discs large 5, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000152">
        <property type="entry name" value="disks large homolog 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006907">
        <property type="entry name" value="DLG5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035537">
        <property type="entry name" value="DLG5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053004">
        <property type="entry name" value="MAGUK_Signaling_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46360">
        <property type="entry name" value="DISKS LARGE HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46360:SF1">
        <property type="entry name" value="DISKS LARGE HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16610">
        <property type="entry name" value="dbPDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04822">
        <property type="entry name" value="Takusan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="704"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="788"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="1348"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="1508"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1599"/>
            <end position="1667"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="6">
        <location>
            <begin position="1778"/>
            <end position="1904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="906"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1044"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1218"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1470"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1695"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="162"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="305"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1071"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1250"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1274"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1292"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1317"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1334"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1477"/>
            <end position="1495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1904" mass="213421" checksum="49E36FF2297E1B41" modified="2024-11-27" version="1">MAGEWWRLAGEWLENGGVWLGNGGVWLGNGWGMVAYGCGMVAYGCGMVAYGCGMVAYGWRMVAYGWGMAGEWWRLAGEWLENGGVWLGNGWRMVAFGWGMAGEWWRLAGEWLENGGVWLGNGGVWLENGGVWLGNVRCCVFLVAASSPPPAHVEKRAVSEKLDSVVLQLRQVTRERDELRKRLALSSPGTTFDDCRPSVKSAHDYERLKLQCMKAMADLQSLQNQHTQTLKKCEEAMKEADFYHTLHGRVLDEQAGLRAELDAVRQEYTQLLHTCDHLRRIHDDDQREVADMRLQQQVLRESSSSDVVNKLYDAAMEKLEALRKEYDALQKRYSEKTACQGADAGRLEQLEAENRRLQKQLDALLKQREIHAHPQQHYSTRRLETVQQDFSKLSVENSDLQREVERLQAEVTRYKSVQLRAVQDADRLREERDSVLNEYRLIMSERDQVIKEVERLQTGLEAAEAKLKNTSSERRVATEELEALRQELALALLDRDRAICERADLLERYSHEVQDKAETQKELSQACKDIETAREERDVARKERTEAIIQRDQLLCEYYQARQKQDSATVDMERVSKEIEVLRKQCEAVSQELKEAVQEAEVAKCRRDWAFKERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKRKNELSRELKELKERLEEEQDKEARYQQLMAHNSHDSAIDTDSLEWETEVVEFERESDMDLKALGFDVAEGVNDPYLPGDCGIFVTKVDKGSVADGRLRVNDWLLKINDVDLANKDKKQVLKAVLNGGGVINMVVRRRRSLGGRIITPVHLNLTGNKDSGVVLESGMYVASITPGSPAARDASLCPGDRLLAINGIAVDNKSLSECEALLRDCRESLSLSIMKVFPHSVCGLSLSESVRECEHGSNGKVPPLEVHTRNCRSQPLNSSSSSTTHSDIYCGQRGAPCPTEAYHELCYQRPDPSKRPLTFHPIASGDGITVETVLEKGIASQKNSGGTWPKVMASAVATPEIAPPLSIFKTPKKRKTIFDVDIFKRPETPSKLGEYITLAHAGSRPQGVLQSPPEPPKRSDSIKFKHKQQGSSASDSTITTGLDRNGNLLQRQNSGDAGTPTPRRACDEDVVRHAVDELEIRKAKSSSASTQRRALTPLQIPIPLQGQDDTCPDVSEVLRFSPQRSQRHSMGFFPPGHHPPSPTHPPHRALSPCPAFTTVMRKPVYTAWSHWVTNNSPTAHPSPHHQGRQRSADLSDQSSRSRIHHNTHSLPSSARQGLSQYRTERIKIPSTPRYTRSTQGSDRGSHSEGSSPSLVTPPLSPLSSDAASFTSSQSHGSLSTQPRLSVSPAPIGDRRKDRPYTEEPRSVTIPKGAEPLGISIVGGENGGIFVSKVTGGSIAQRHNLEFGDQLLEVNGINLRNATEQQARLIIGQQCDTITILAQYNPHMYQPGNHSRSSSRMESVSACSTPVSNGAGTPDACSTIDTLSEQDEGTMTPPSKNTTPATSPRNSWIRHSSAGGRLVEPRQVTVRKGHVELGVKICGGNLTGIYVESLEEKSPAGGTDGLQPGDFILEFCSVSLRNKTTEEAYMEMLKPAETVTLRVQNRPEDWRRIRNTPGDGFYVRALYDRVGESEQELSFKKDDILYVDDTLPGGNFGYWLAWQLDESAQKLARGQIPSKYMMDQEFPRRCSITEGKDEGKTVSAAARRSFFRRKLKHKRSGSRDGKDAPAPDSISTDSITFAEDCISLAYQRVLKLESTNRRPVLVLGPLVEPIKDMLVREAPGKYCRCLLEVMKASQQAIERGVKDCLFIDYKRRSGHFDVTTVASIKEIIEKDCHCLIDIAPHAIERLHSVNIYPIVIFIRYKNAKQIKEQKDPVYLRDKVSQKHSKEQFEAAQKMEQEYSKYFTVLSCCTQVDSPKHHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KLI8</accession>
    <name>A0AAR2KLI8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Myosin-9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Myosin heavy chain 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Myosin heavy chain, non-muscle IIa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Non-muscle myosin heavy chain IIa</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasmic vesicle</location>
            <location evidence="15">Secretory vesicle</location>
            <location evidence="15">Cortical granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000041981.1">
        <property type="protein sequence ID" value="ENSPNAP00000063277.1"/>
        <property type="gene ID" value="ENSPNAG00000014179.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155632"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060473">
        <property type="term" value="C:cortical granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030029">
        <property type="term" value="P:actin filament-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1590:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000003">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000008">
        <property type="entry name" value="Myosin heavy chain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000175">
        <property type="entry name" value="Myosin heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000007">
        <property type="entry name" value="Myosin heavy chain, non-muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.270.10:FF:000001">
        <property type="entry name" value="Myosin heavy chain, non-muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000009">
        <property type="entry name" value="myosin-11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF16">
        <property type="entry name" value="MYOSIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 20" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 20" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="23">
        <location>
            <begin position="25"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="22">
        <location>
            <begin position="79"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="20">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1924"/>
            <end position="1957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1999"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1924"/>
            <end position="1936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="2013"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="172"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2024" mass="234604" checksum="F9A937B9432391E5" modified="2024-11-27" version="1">MSAADKYLYVDRSVVNNPLAQADWASKKLVWVPSERLGFEAGSVKEEHGDEVLVELADSGKKVRVNKDDIQKMNPPKFNKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKTKKDQNSSVLSHGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAREERTFHIFYYMLSGAGDKIRTELCLEGYSKYRFLSNGNVTIPGQQDKDMFTDTMEAMRIMGFSPEEHIGLLRVVSSVLQLGNMSFKKERHSDQASMPDDTAAQKVCHLMGMNVTDFTRAILSPRIKVGRDYVQKAQTQEQAEFAVEALAKATYERMFRWLVMRINKALDKTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWSFIDFGLDLQPCIDLIEKPASPPGILALLDEECWFPKATDKSFVEKVLQEQGTHPKFFKPKKLKDEADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLNQSTDKFVSELWKDVDRIVGLDKVAGMSELPGAFKTRKGMFRTVGQLYKEQLSKLMATLRNTNPNFVRCIIPNHEKKAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACVLMIKALELDPNLYRIGQSKVFFRAGVLAHLEEERDMKITDVIISFQAWCRGYVARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLMHSLTALTRLKLMQLIKELLALRNSGLLLWLRFALYHAPEVQFKLSAWALMSVFRPLVLQVKPLLQVSRQEEEMQAKEDELNKVKEKQLHAEKQLEDMEVKQQQLMAEKLALQEQLQAEMDLCAEADEMRNRLVAKKQELEEILHDLEARVEEEEERANHLQTEKKKMQQNITDLEQQLDEEEAARQKLQLERVTLEAKLKKVEEDVMVLDDQNNKLSKEKKLLEERVAEFTTNLAEEEEKSKSLQKLKNKHEAMITDLEDRLRREEKLRQELEKNRRKLEGDSTELHDQIAELQAQIAELRAQLAKKEEELQEALARIEEEAAQKNLAQKKIRELEAQLSELQEDLELERAARTKAEKHRRDLGEELEALKTELEDTLDSTAAQQELRAKRETEVAHLKKTLEDEAKVHEQVVVEMRQKHGQAFDELNEQLEQAKRNKASVDKAKQALESERNELQIELQTLMQGKGESEHRRKKAEAQVQELQVKHAESEKQRAELAEKVTKLQAELDNVNSLLSEVEGKSIKATKDCSAVESQLQDVQELLQEETRQKLSFGTRLRQLEDEQNGLKEQLEEEEEARRNVEKQLQAAQAQLGELRKKMEVEAGSLENAEEGKKRVQRDLEAVTQRLDERSLAYDKLDKTKTRLQQELDDLLVDQDHLRQIVSNLEKKQKKFDQMLAEEKSISARYAEERDRAEAEAREKETRALALARELETISDMKDELDRANKMLRAEMEDLVSSKDDVGKSVHELEKSKRGMEQQLEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQYERDLAGKDEMGEEKKRQLVKQVREMEMELEDERKQRSVAVAARKKMELDLKELEAAIDQANKNREEALKQLKKLQAQMKDLLRELDETRLSREEILAQSKENEKKVKSMEADMIQMQEELAAAERMKRQAQQERDELQDEINNQASKTALAVEEKRRLEARIAQLEEELEEEQGNTELLNDRLKRTLLQVDQVNVELTAERSTSQRLEGARSQFERQNKELKLKLTEMEGTVKSKYKATIAALEAKIAQLEEQLDVETRERQSATKLVRRTEKKLKEVVLQVDDERRNSEQYKDQTEKLNSRMKQLKRQLEEAEEEAQRANANRRKLLRELEDATESADAMNREVTSLKSKLRRGDLPFTMRRVVSRGGVESDEESEAKSETPEPKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNR8</accession>
    <name>A0AAR2KNR8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 17">Alkaline phosphatase</fullName>
            <ecNumber evidence="4 17">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="4 17"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNR8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049611.1">
        <property type="protein sequence ID" value="ENSPNAP00000063761.1"/>
        <property type="gene ID" value="ENSPNAG00000018293.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183063"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000008">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF37">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 15 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15 17" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="14">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="540" mass="60202" checksum="CB13249530A6863C" modified="2024-11-27" version="1">REREREREREGEREREREREVITLKSVFLFLRFWLIAEWEADPSFWNDQARRTLEDALKLQPRVHRAKNIILFVGDGMGVSTVSAARILRGQMEGQSGEETVLAMDTFPYLALSKTYSVDKQVADSASTATAYHCGVKANSKTVGVSANAVAYECNTTFGNEVYSVLHRAKAQGKSVGIVTNTRVQHASPAAAYAHSVSRSWYSDVDLPSAARRQGCTDISTQLVTNTDIDVILGGGRMYMTPKGTPDPEYPSSSSRKGDRKDKKNLIEVWLNDRKDRNAQYVWHKEQFNAVDVKTTDCLMGLFEPKDMRYEVFRNRSRDPSIVEMTEKAIQILSKNPKGFFLFVEDEGRIDHGHHDGIAKLALTETVMFDRAVQRASELTKESETLTVVTADHSHVFTFGGNTPRGNPIFGLAPKKADDRLPFTSILYANGPGYVHVNGTRANVSAVDYFDEEYMQQAAVPLDAETHGGEDVAIYAKGPMAHLFHGVKEQHYVAHALAYAACLEPYTHCPPDPLQSGTHGHPLSTLLISVTSLICLVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KS71</accession>
    <name>A0AAR2KS71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 22 member 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Organic anion transporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Renal organic anion transporter 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Basal cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KS71"/>
    <dbReference type="Ensembl" id="ENSPNAT00000088094.1">
        <property type="protein sequence ID" value="ENSPNAP00000064971.1"/>
        <property type="gene ID" value="ENSPNAG00000013213.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154901"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17446">
        <property type="entry name" value="MFS_SLC22A6_OAT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000023">
        <property type="entry name" value="Solute carrier family 22 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="50"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="541" mass="60588" checksum="0C763DF6A125E6DE" modified="2024-11-27" version="1">MAFADLLEQVGSTGHFQIMHVTLLSLPILMMASHNLLQNFVAAVPSHHCVAHTNLSGHLLDPEDTLLISIPLDQQGRPERCRRYTHPQWHLLHTNSSKELEWENEENGINMQGCVDGWKYNMTEMTSTIITEWDLVCDMRSLKQMGQTIYMGGVLVGAMIFGGLSDRFGRRVLLLISHLMMAVAGTCAAFATSFSLFCLFRFFCGMALSGLVLNSFSLIVEWIPTRVRTVVGTGTGYCYTTGQLILAVVAYNIRDWRWLTLAVSLPFYVSFLYSWWFLESARWLVLNKKPDQAVKNLKAVARMNGRRDEADKINIEMLQQSMKKEMSCSQISHSVLDLLRKRTMRTITVCLSAVWFSTSFAYYGLSMDLQKFGVNIYLIQVIFGAVDIPAKIIVTVSMSIIGRRISQCAALMLAGITILINMLVPYELQTLRTSLAVIGKGCLAASFNCCYLYSGELYPTVIRQNGMGWVSMMARLGAMVAPLVLLIGEAVAWFPGFIYGGAPILSGIFAYFLPETLATPLPDTIQDKEEMHTIQHTIQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KU33</accession>
    <name>A0AAR2KU33_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myosin light chain kinase, smooth muscle</fullName>
            <ecNumber evidence="5">2.7.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Telokin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.18" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017579603.1">
        <property type="nucleotide sequence ID" value="XM_017724114.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KU33"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059139.1">
        <property type="protein sequence ID" value="ENSPNAP00000065984.1"/>
        <property type="gene ID" value="ENSPNAG00000021621.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443440"/>
    <dbReference type="CTD" id="492576"/>
    <dbReference type="GeneTree" id="ENSGT00940000157879"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004687">
        <property type="term" value="F:myosin light chain kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055013">
        <property type="term" value="P:cardiac muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000175">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000080">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF1">
        <property type="entry name" value="MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="18" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="189"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="322"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="418"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="552"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="889"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="277"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="503"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="853"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="982"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="100"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="861"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1002" mass="110263" checksum="941A2AB18F27C3E8" modified="2024-11-27" version="1">MDFRAALKPRPGPKLGSEIKTNTPTKVDFRSVLGKKEGSSPKPAPPEPAPSSAPADFRSILNKKKTPEPEKSGAKEPAPTPAVTKQDKQSGINGMNGTVDNKKTSLVEKVGTEKKTAGGTAEKKSIPAEKLSPEKKTDGGIVESKNKTVDGGINQKKGTNVDGSSAEKKSVPISVDGGDGGKKGSGKAPSFTQTLNDVTVVDGERLRLECTVTSDSPAVITWSLDGKVVKPSKFIVLSQEGGKCSLTIDKALPEDEGRYTCKAENAEGKAECSCMVQVDDPSDPSAEKKNRKSSSSAPTTENEARIKKKPAPKSPTKQGSPPQILQFPGDLKIRAGEKVELLCNFGGTAPITCTWLKFKKLIQAGSGGVTIETKENSSQLTIAASDQDHCGCYTLELQNKYGTKQASLNLTVVDKPDPPAGVPAASDIRKSSLTLSWYGPTYDGGSIVQSYNLEIWNSVDDKWSTLTSCNSTSYHIQELLSDRQYKFRVRAVNMYGIGEPSAESEPVTVGVQVEPEKKEEEAEATVSDDDSDKEPEYRDVVIKKDCSVKDFYDVEDRLGTGKFGQVFKVVEKSTKKVWAGKFIKAFSQKEKENVRQEISIMNSLHHPKLVQCMDAFEGKSDMVMVMEMISGGELFERIVDEDFELTEREVIKYMLQIIDGVQFIHKQGIVHLDLKPENIMCVNKTGSKIKLIDFGLARRLEDSGALKVLFGTPEFVAPEVINYEAISYPTDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFEDEAFDEISDQAKDFISSLLKKHMKARLSCAQCLEHTWLKQDTKNMEAKQLSKERMKKYILRRRWQKTGNAVRAISRFSSMGMLAGIGPKKSSPTEDNKPEEPPAPFLESAEDENHTPAPPTFSLVIQDVEVVEGSAARFDCKIEGYPDPEVVWYKDDQPIKETRHFQIDYEEDGHCSLVISEVCPDDDAKYTCKAVNSLGEASCTAELMVEFMQEEEGEGEEEEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KUG9</accession>
    <name>A0AAR2KUG9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Esterase OVCA2</fullName>
            <ecNumber evidence="5">3.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">OVCA2 serine hydrolase domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">OVCA2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Exhibits ester hydrolase activity with a strong preference for long-chain alkyl ester substrates and high selectivity against a variety of short, branched, and substituted esters. Is able to hydrolyze ester bonds within a wide range of p-nitrophenyl derivatives (C2-C14) in vitro, with a strong preference toward substrates of &gt;8 carbons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a carboxylic ester + H2O = an alcohol + a carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:33308"/>
            <dbReference type="EC" id="3.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LovG family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_037402929.1">
        <property type="nucleotide sequence ID" value="XM_037547032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KUG9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041500.1">
        <property type="protein sequence ID" value="ENSPNAP00000065781.1"/>
        <property type="gene ID" value="ENSPNAG00000036600.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411114"/>
    <dbReference type="GeneTree" id="ENSGT00390000003541"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106435">
        <property type="term" value="F:carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032526">
        <property type="term" value="P:response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000073">
        <property type="entry name" value="esterase OVCA2 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
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                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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</entry>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Winkler S."/>
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                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2883"/>
            <end position="2895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="4100"/>
            <end position="4140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="4150"/>
            <end position="4159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="4198"/>
            <end position="4218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="4233" mass="467905" checksum="1E99A5A962116853" modified="2024-11-27" version="1">[encoded data, 4233 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4N8</accession>
    <name>A0AAR2L4N8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATPase inhibitor, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP synthase F1 subunit epsilon</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Forms an alpha-helical dimer with monomers associated via an antiparallel alpha-helical coiled coil, leaving each N-terminal inhibitory region accessible for interaction with an F1 catalytic domain. The inhibitory N-terminal region binds the alpha(ADP-bound)-beta(ADP-bound) (ATP5F1A-ATP5F1B) interface of F1-ATPase, and also contact the central gamma subunit (ATP5F1C). This dimeric state is favored by pH values below 7.0, and at higher values the dimers associate to form inactive homotetramer, where the inhibitory region is occluded, masking its inhibitory activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATPase inhibitor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567069.1">
        <property type="nucleotide sequence ID" value="XM_017711580.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L4N8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056390.1">
        <property type="protein sequence ID" value="ENSPNAP00000071568.1"/>
        <property type="gene ID" value="ENSPNAG00000032162.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108435613"/>
    <dbReference type="CTD" id="100037369"/>
    <dbReference type="GeneTree" id="ENSGT00940000165091"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000004">
        <property type="entry name" value="ATPase inhibitor A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000003">
        <property type="entry name" value="ATPase inhibitor B, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007648">
        <property type="entry name" value="ATPase_inhibitor_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48417">
        <property type="entry name" value="ATP SYNTHASE F1 SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48417:SF1">
        <property type="entry name" value="ATP SYNTHASE F1 SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04568">
        <property type="entry name" value="IATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64602">
        <property type="entry name" value="F1 ATPase inhibitor, IF1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="107" mass="12183" checksum="CF1746D206E41F57" modified="2024-11-27" version="1">MARLLRSSLRGYYMTQIRMSSDQLGELGKGAGKGGGGGGSVREAGGAFGKKQAAEEERYFKQKEKEQLAALRQHHKDEIEHHKKEIERLQREIDRHKGKIRKLNHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L699</accession>
    <name>A0AAR2L699_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ankyrin repeat and sterile alpha motif domain containing 1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ANKS1A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L699"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057083.1">
        <property type="protein sequence ID" value="ENSPNAP00000072065.1"/>
        <property type="gene ID" value="ENSPNAG00000021680.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155806"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01274">
        <property type="entry name" value="PTB_Anks"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09499">
        <property type="entry name" value="SAM_AIDA1AB-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09500">
        <property type="entry name" value="SAM_AIDA1AB-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000045">
        <property type="entry name" value="Ankyrin repeat and sterile alpha motif domain-containing protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000099">
        <property type="entry name" value="ankyrin repeat and sterile alpha motif domain-containing protein 1B isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033635">
        <property type="entry name" value="ANKS1/Caskin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041880">
        <property type="entry name" value="SAM_ANKS1_repeat1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041882">
        <property type="entry name" value="SAM_ANKS1_repeat2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174:SF4">
        <property type="entry name" value="ANKYRIN REPEAT AND SAM DOMAIN-CONTAINING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24174">
        <property type="entry name" value="ANKYRIN REPEAT AND STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="73"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="702"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="773"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="7">
        <location>
            <begin position="941"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="322"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="378"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="589"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="870"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1075"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="457"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="471"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="600"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="610"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="879"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1169" mass="127350" checksum="A285AED09DEE58CE" modified="2024-11-27" version="1">MGKEQELLEAARTGNLAAVEKLLGGKRQSAGAAGAGGGSGNAGGGSGHGSSSHPLSSLLSIWRVPNVNCVDSNGYTPLHHAALNGHSEVVELLLRNEASTNVADSKGCYPLHLAAWKGDQRIVRLLVHQGPSHPKLNEQTSLDLREFKRCGPFDPYINAKNNDNETPLHCAAQYGHSEVVRLLLEELTDPTMRNNRFETPLDLAALYGRLEVVKLLLCAHPNLLSCNTRKHTPLHLASRNGHLLVVEVLLNAGMDINYQTEKGSALHEAALFGKTDVVQKLLSAGISVNIVDSKNLTALDIVREMPSQKSRQIAALIQAHMSGHPPDQALPPPPPPPPQEKLSPRRKGDMEQQVNDLMSGMEESPYEALFEASSCHSLDSLTSGRSSDRDSGRPDSDAGRSFPKDRAPPLPSQLGPGQEEELSSEAVYTSSSVDERLEEEDDDHPYETLHMARTKHPATDTHTQTDKDVSAKSSNSLLPQQQKPTAPTDLRPPGSNTHSLKPPGPPSGTGRPSGENSADLSASVPEQFTGLLHGSSPVVDSRGGEEAFRLKSSQSSGTLTSVTSSAPPPTSSVAMAAILTDPDPKAIYATVNKEPRDSPSRASPSAGRMRPGDLKLARSLSKSDSDLLVSPPSEEEAGLGGRSESVSNCSAAKRRLEKSPSFTSEWDEIEKIMTLIGAGIDFSKDQESSASGGVSRLLDQPVGDWLEHVGLPQYESKLLLNGFDDLRFMGSNVMEEQDLREIGITDASHRRKLLSAARTLPKVKALGCDGSTSLSSWLDSLGLTEYLHNFLTSGYRTLECVKNLWELEIVNVLKITLLGHRKRIIASLAERPYEEPPVKPPRLSQIRCPELMSQSSSPLSHSELYTHRSMDPLLPAGDSGRRKGGDSEYDLSIKPRPQEKYEDRPPESRLTLRPPSLAAPYAPVQNWHHQPEKLIFESCGYEANYLGSMLIKDLRGTESTQDACAKMRRSTEQMRKVPTIVLSITYKGVKFIDAANKNIIAEHEIRNISCAAQDPEDLCTFAYITKDLQTSHHYCHVFSTVDVNLTYEIILTLGQAFEVAYQLALQAQRNKQQQGSVGSEVIDTKSSRPVPKPRGSVRKSGDCPVYGNRCCYCNCPVHRPVLSPFCSCAELQVDSVNVDETPSQSGAAWLLDPKDPKRTVSTKYETTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L6A7</accession>
    <name>A0AAR2L6A7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NDUFA4 mitochondrial complex associated like 2a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L6A7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052583.1">
        <property type="protein sequence ID" value="ENSPNAP00000069901.1"/>
        <property type="gene ID" value="ENSPNAG00000031663.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161040"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256:SF5">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 4-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12391" checksum="F1E9D901EA3B7071" modified="2024-11-27" version="1">MALFQFKFGVRVAVLKVRYFNAISSESRTTYDPLRSLIPQFFFICLGMGGATMYLIRLARGPHVTWNKTSNPEPWNQLSPTYQYKVCSNENKQKGKSFQYWCSQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LA68</accession>
    <name>A0AAR2LA68_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FAF1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LA68"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085529.1">
        <property type="protein sequence ID" value="ENSPNAP00000073478.1"/>
        <property type="gene ID" value="ENSPNAG00000017029.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154831"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02990">
        <property type="entry name" value="UAS_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14413">
        <property type="entry name" value="UBA_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17129">
        <property type="entry name" value="Ubl1_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17130">
        <property type="entry name" value="Ubl2_FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01771">
        <property type="entry name" value="UBX_UBXN3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000089">
        <property type="entry name" value="Fas associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000061">
        <property type="entry name" value="Fas associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033043">
        <property type="entry name" value="FAF1-like_UBX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049483">
        <property type="entry name" value="FAF1_2-like_UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044541">
        <property type="entry name" value="FAF1_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF96">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21021">
        <property type="entry name" value="FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="589"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="670" mass="75320" checksum="053F0DFDE896645A" modified="2024-11-27" version="1">MSQVKGYVITNLRQQILLDLTFLFQACTGIDNIAEAITLLELNNWDLVAAINGVIPQENGILQSEGSQTGPFGDNSQAASTAPTAPPSRAASSSPPSSSSSSSAFCPVLPSRQIVERQPRMLNFHVEYRTRSVEVVLEDASTVGDIKQILETELQVPVSKMQLKGWKSGDVSDSTVLRSLHLPKNNSLYVLTPDIAPSATTSQSSVQQESLNQNYLLIISHREAQREYSLNFPATRTIQEVKRNISDLTNIPVRHQQWDGWPASASDDSMTLASSGISFPCHHLSVCRRSSPATALEPTEECTDVHMVSDSEGDDFEDASEFGVDESEIFGMGSSSCRKSPMMPENSENEGDALLHFTAEFSSRYGDCHPVFFIGSLEAASQEAFYGKARDRKLLAIYLHNDDSVLSNVFCSQMMCAESIVSYLSQNFITWAWDVTKEANKARLLTMCTRHFGSVVAQTIRTYKTDQFPLLLIVMGKRTSNEVLNVIQGNTTVDELMMRLMGAMEIFTAQQQEDIRDEDEREAREMVKREQDEAYRLSLEADRKKREAQEREEAEQVRQERIRKEQEEEREAIRLSLEQALPPEPNEEEGEPVSKLRIRTPSGEFLERRFLGSCKLQVLFDFVASKGYPSDEFKLLTTFPRRNITQLDPAWTLLEAKLFPQETLFLEAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBN8</accession>
    <name>A0AAR2LBN8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCR4-NOT transcription complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CNOT10</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CNOT10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBN8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077048.1">
        <property type="protein sequence ID" value="ENSPNAP00000072072.1"/>
        <property type="gene ID" value="ENSPNAG00000021374.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001827"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000092">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039740">
        <property type="entry name" value="CNOT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12979">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12979:SF5">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="484"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="611"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="721"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="757" mass="83207" checksum="5C1342D34B82D94C" modified="2024-11-27" version="1">NLADVADQANDMKHDSADSPGVSDQEKEMATNAFEAFTAGNYDESLKQLESLQELNKEDYKIAMNKAVAEFYKTGQATTGTLKQILMTIKNQVHSAVEDIDGLDDVENSILYYNQAIIHYHMRQYSEAISIGEKLYQFLEPFGIAVRWHGLMGFSVLEEKFAQAVCFLLVDLYLLTFQPEKALHLLTMLEKLSVQGSSKNGKGDGNNSTAKESAAQKAEFAAMIEAAKSRIHHYKVRAYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSSNIAEHPGPLKTGECVRCMFWNNLGCIHFAMGKHNLGIFYFKKALQENDHTCAQLGDAKKFTGIPMCALLANKRYELLYNCGIQLLHIGRPLAAFECLMEAVQVYHSNPRLWLRLAECCIAANKGSSEQETKGLPSKKGIVQSIVGQGYHRKIVLASQSTQNTTYSEGQSAAIPVASMEFAAICLRNALLLLPEHQQHESKPDNGSKSSSQSGSTESGSENSDACSGKGQEGDKFIPAAPSSPLRKQEVENLRCCILACSAYVALALGDNLMALNHADKLLHQPKLSGSLKFLGHLYAAEALISLDRISDAIAHLNPENVTDVSLGVSPSEQDQGSDKGDLEPVETSGKQTPLCYPSTVSSARAMMLFNLGSAYCLRSEYEKARKCLHQAASMVNPKEIPPEAILLGVYLELQNGNTQLALQIIKRNQLLPSVKPSSSPDLRKKPPPFQPGQPVQPIKMPSSFTQVQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBX9</accession>
    <name>A0AAR2LBX9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBX9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045483.1">
        <property type="protein sequence ID" value="ENSPNAP00000074108.1"/>
        <property type="gene ID" value="ENSPNAG00000033417.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144271"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF87">
        <property type="entry name" value="ARGININE ESTERASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043557573" description="Peptidase S1 domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="63" mass="6941" checksum="437021DAE7FB47B6" modified="2024-11-27" version="1">MALISLMLLAAVLPYLVGIINGTEAKPHSRPYMVSLQMGGRHMCGGFLVSDRYVMTAAHCYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LTE0</accession>
    <name>A0AAR2LTE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA methyltransferase 10 homolog B</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RNA (guanine-9-)-methyltransferase domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA (guanine(9)-N(1))-methyltransferase TRMT10B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TRMT10B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LTE0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052052.1">
        <property type="protein sequence ID" value="ENSPNAP00000077989.1"/>
        <property type="gene ID" value="ENSPNAG00000020605.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063169"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18100">
        <property type="entry name" value="Trm10euk_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047911">
        <property type="entry name" value="Trm10_B_MTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF19">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043736770" description="tRNA methyltransferase 10 homolog B" evidence="12">
        <location>
            <begin position="16"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="13">
        <location>
            <begin position="118"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="326" mass="37416" checksum="5FDC1DD2CCD156D1" modified="2024-11-27" version="1">MFFLAFGFCSRAIAAALHSVFEMNEKLCSSVAQQMILTEDSVSDLMELLSVDVELSSSAADREETLCSKNVMRKQRNWERRLEAKRRKRKEEKQRRKQSQQEKDFVPQFSKRVVKAITKERLEEARTTGPRLCVDLSMTDCMSHKEISRLACQIRRLYGSNKKTQQPFHLFLTDMKKDGLLYRECIRMNAGFLNYLIDVTEDSWIELFPSEDIIYLTPDASEALEQVDDDKVYILGGLVDESIQKKISYTRAKELGVQMARLPIDEYMVKKPNSKNFYSKILAINQVFEILLTYRDTGSWTTALSAGIPVGKGYVASSEALPEGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LU14</accession>
    <name>A0AAR2LU14_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">creatine kinase</fullName>
            <ecNumber evidence="2">2.7.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>creatine + ATP = N-phosphocreatine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17157"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57947"/>
            <dbReference type="ChEBI" id="CHEBI:58092"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:17158"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LU14"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063475.1">
        <property type="protein sequence ID" value="ENSPNAP00000077826.1"/>
        <property type="gene ID" value="ENSPNAG00000004654.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182772"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000026">
        <property type="entry name" value="Creatine kinase B-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000001">
        <property type="entry name" value="Creatine kinase M-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF23">
        <property type="entry name" value="CREATINE KINASE B-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="12">
        <location>
            <begin position="73"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="13">
        <location>
            <begin position="187"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="354"/>
            <end position="358"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="382"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="443" mass="50087" checksum="CEC3763544ABB8E5" modified="2024-11-27" version="1">MKTIYSRYVWRVFSSKRVDKIGFSTKPSRCADYFKAERVRTSFCCNWHYFLSHPRRVQLASTMPFGNSHNQLKMKYSAEAEYPDLSKHNNHMAKVLTQAMYERLRGKQTPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEETYEVFKELLDPVIEDRHGGYKPTDKHKTDLNPGNLKGGDDLDPNYVLSSRVRTGRSVRGFSLPPHCSRGERRAIEKLSVEALGALSGDLKGKYYALKNMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTKIEELFKNKGHAFMWNEHLGYVLTCPSNLGTGLRGGVHVKLPNMSKHSKFEEILKRLRLQKRGTGGVDTAAVGGVFDISNADRLGFSEVALVQMVVDGVKLLIEMEKRLEKGQSIDDLIPAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LU32</accession>
    <name>A0AAR2LU32_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NR LBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NR0B1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear hormone receptor family. NR0 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550082.1">
        <property type="nucleotide sequence ID" value="XM_017694593.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LU32"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086006.1">
        <property type="protein sequence ID" value="ENSPNAP00000080070.1"/>
        <property type="gene ID" value="ENSPNAG00000035217.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108425711"/>
    <dbReference type="CTD" id="190"/>
    <dbReference type="GeneTree" id="ENSGT00390000015719"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07350">
        <property type="entry name" value="NR_LBD_Dax1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000031">
        <property type="entry name" value="Nuclear receptor subfamily 0 group B member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033544">
        <property type="entry name" value="NR0B1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24081">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 0 GROUP B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24081:SF1">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 0 GROUP B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="264" mass="28837" checksum="C5480B8CBD60FA13" modified="2024-11-27" version="1">MAHSDSCHCGGQRPHSSILYSILKSDGQAALHAPRNPRLAVSMHACACGAKKQVALRSPHTTCKAASAVLVKTLKFVKNVPCFRELPIDDQHALVRSSWAPLLVLGMAQDRIDFETAETAEPSMLQRILTSGQQGEQESARARGHTGVALADVQAIKMFLSKCWGLDISTKEYAYLKGAILFNPDVAGLQCHRYIQGLQSEAHQALNEYVKMIHGGDTARFAKLFIALSMLRSINANVVAGLFFTPVIGTVNMEELLLDMFYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LWU7</accession>
    <name>A0AAR2LWU7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EXPERA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">EBPL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554526.1">
        <property type="nucleotide sequence ID" value="XM_017699037.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LWU7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068326.1">
        <property type="protein sequence ID" value="ENSPNAP00000078811.1"/>
        <property type="gene ID" value="ENSPNAG00000032579.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108428215"/>
    <dbReference type="CTD" id="84650"/>
    <dbReference type="GeneTree" id="ENSGT00530000063715"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047750">
        <property type="term" value="F:cholestenol delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007905">
        <property type="entry name" value="EBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207:SF1">
        <property type="entry name" value="EMOPAMIL-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207">
        <property type="entry name" value="STEROL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="8">
        <location>
            <begin position="45"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="208" mass="23704" checksum="56A9FE29B097290E" modified="2024-11-27" version="1">MAEAVSVDEPALFTPVTGISLLACAAQFAVGYIFAQIWGRKCSSTDRWVLVWLFYDAIVHITLEGPFVYMSLVGTVAASDSVLAELWKEYGKADQRWLHSDPTIVSLELLTVVLDGFLALFLVYAVVKDKHYRHFVQITLCVCELYGGWMTFCPDWLIGSPNLNTSNWLYLWVYLVFFNGVWVVVPGLLLYQSWNDLQRSHHSLKKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LYA8</accession>
    <name>A0AAR2LYA8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase</fullName>
            <ecNumber evidence="14">2.4.3.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-[N-acetyl-alpha-neuraminosyl-(2-&gt;6)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81643"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="Rhea" id="RHEA-COMP:19720"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="ChEBI" id="CHEBI:231970"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:81644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-O-[N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-{alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-[alpha-Neu5Ac-(2-&gt;6)]-alpha-D-GalNAc}-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81659"/>
            <dbReference type="Rhea" id="RHEA-COMP:14417"/>
            <dbReference type="Rhea" id="RHEA-COMP:16763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:139598"/>
            <dbReference type="ChEBI" id="CHEBI:156398"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:81660"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = a beta-D-galactosyl-(1-&gt;3)-[N-acetyl-alpha-neuraminyl-(2-&gt;6)]-N-acetyl-alpha-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11136"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="ChEBI" id="CHEBI:140764"/>
            <dbReference type="EC" id="2.4.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:11137"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.3" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017573375.1">
        <property type="nucleotide sequence ID" value="XM_017717886.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LYA8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050529.1">
        <property type="protein sequence ID" value="ENSPNAP00000081568.1"/>
        <property type="gene ID" value="ENSPNAG00000021751.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439451"/>
    <dbReference type="CTD" id="407706"/>
    <dbReference type="GeneTree" id="ENSGT00940000164863"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001665">
        <property type="term" value="F:alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000015">
        <property type="entry name" value="Lactosylceramide alpha-2,3-sialyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45941">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45941:SF4">
        <property type="entry name" value="ST6 N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043456697" description="alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase" evidence="17">
        <location>
            <begin position="23"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="452" mass="52271" checksum="5334C7FB63214530" modified="2024-11-27" version="1">MTGFLPQRRLMLLFLLLTAVLGIYILHANMDRKRWLQDDQHIVPDHNGLLYSKDDSNSSLDYRLEVDLTRAKQEQQRKDAQSNTLQTAVRASLQQNLNSSDSEKRESNLTKASEELLKQAEPSEPDFFGDRYATDVPLQTSCSSSIRRKVSQTPFAKKFLENVPVLQWAKHFSEQEYQRLSRYQGAHGWGSVDMDVLKNSLALLNTTANRLMFDDWERRGNRSHCIRCAVVGNGGILNGSKKGREIDQHDYVFRTNGAVIKGFEEDVGNRTSLYTFSTNTLRNSMRSYAGVGYRGPPLDKETKYVFLPDHDRDYILMRAAATHTPIEKGPEKSAKPPSYFGSNVTVEKFKMYHPDFIRYIRNRFLCSHTLNTRYKNIYRPSTGAVMLLAALHTCDQVSAYGYMTSDYNKYSDHYYDKTRNPVRFYANHDLRMELMLWQQLHQAGLIQLYMRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M069</accession>
    <name>A0AAR2M069_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tripartite motif-containing protein 16-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017575173.1">
        <property type="nucleotide sequence ID" value="XM_017719684.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M069"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077621.1">
        <property type="protein sequence ID" value="ENSPNAP00000080384.1"/>
        <property type="gene ID" value="ENSPNAG00000018595.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440677"/>
    <dbReference type="GeneTree" id="ENSGT01150000286899"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="154"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="365"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="209"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="63399" checksum="E23472A9E196E58C" modified="2024-11-27" version="1">MAEAFLTARDSFSCPICLDLLKDPVTIPCGHNFCLDCIEDCWDEDDQRGVYSCPQCRHSFTPRPVLNKNNLIAEMVEKIKTMKFQAPPGSPADSPAGSLAGSGDVECDVCSGTKLRAVKSCLVCLASYCEAHIQPHYESPAFKKHKLVEASMNLQDKICSLHDKLLEVFCRTDQKCICLLCSLDEHSGHKTVSAAAEKTEKQRQLGQNREDCKRIIQQKEKDVRELTQVMEDLMRSAQAAVEDSEKIFTELIESIERRRSEVKELIRAQEKTELSQAEDLLKKLEQEIADLRRRDAELEQLSHSEDHIHFLQDYLSLPALPGVTKFYNINTKPQFSIEFVKKLVSEMEQLNWFSKLEIVKISKVHTIRAAEPKTRKEFMKCFCQLTLNPDTMHKNLYLCERNRQVNLTGDNQFNYHGPTTRYPENWCQVLCAEPLSGHCYWEVEISGQVSIAVSYKDSWKTQGSKFVFGSIYSSCSLECFDSNLYFREHQGTKSLGVHPCSSRIGVYVDHSAGILSFYSVSDTMTLLHRVHTTFTQPLYAGFGMKAKKSSVQICDRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M0P3</accession>
    <name>A0AAR2M0P3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Msh homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560863.1">
        <property type="nucleotide sequence ID" value="XM_017705374.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M0P3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044185.1">
        <property type="protein sequence ID" value="ENSPNAP00000080181.1"/>
        <property type="gene ID" value="ENSPNAG00000034091.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108431905"/>
    <dbReference type="CTD" id="30527"/>
    <dbReference type="GeneTree" id="ENSGT00940000164716"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043049">
        <property type="term" value="P:otic placode formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000134">
        <property type="entry name" value="Homeobox protein MSX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050674">
        <property type="entry name" value="Msh_Homeobox_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24338">
        <property type="entry name" value="HOMEOBOX PROTEIN MSX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24338:SF8">
        <property type="entry name" value="HOMEOBOX PROTEIN MSX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="114"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="116"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="239" mass="25936" checksum="7413311A48B9A959" modified="2024-11-27" version="1">MAPSVSMSSGCVGVKSEAEMKPRAAPPLPFSVEALMADRTPSRPDRSAAGTSGSPGMDAVVSPLAVGSSFSVGGIMEVVKAESPERSSWVQSARFSPTSPRRLSPPACPLRKHKTNRKPRTPFTTSQLLALERKFRQKQYLSIAERAEFSSSLNLTETQVKIWFQNRRAKAKRLQEAELEKLKMAAKPMLPPAFGISFPLGTHMPASYGGAHPFQRHAMPVSPAGLYAAHVGYSMYHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M2D0</accession>
    <name>A0AAR2M2D0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRR15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M2D0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061423.1">
        <property type="protein sequence ID" value="ENSPNAP00000082958.1"/>
        <property type="gene ID" value="ENSPNAG00000034665.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000205799"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028237">
        <property type="entry name" value="PRR15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14581">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14581:SF4">
        <property type="entry name" value="PROLINE-RICH PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15321">
        <property type="entry name" value="ATAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="139" mass="15497" checksum="7E85E7CE12EC7C11" modified="2024-11-27" version="1">GDDSGPKTAPWWRSFVGKRRKAARESAAMLEQELGAHTASAPVTTNTSTTKESPTKPEQQEVPQPQQQASEVSTLSNEDTYDDSAVQPTFGEGHSRRNLRVSRSGRFKEKRHMRISLPQNYEDSQNPAGQGETRIPQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M4F2</accession>
    <name>A0AAR2M4F2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M4F2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049682.1">
        <property type="protein sequence ID" value="ENSPNAP00000081746.1"/>
        <property type="gene ID" value="ENSPNAG00000035569.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000207597"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="131" mass="14858" checksum="F72C112813E7D1E1" modified="2024-11-27" version="1">MWVPWTCFAAEYTEVTGYEGRSLRINCHYPPGSEEPGKYFCRLQGFPCPDLIRTTVKNSWDVQGRFALFDNTSARFFTLEIFSLHVNDSGKYWCGVDISLRPDVISGVQITVKGGESPSATVVQLKQLNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M661</accession>
    <name>A0AAR2M661_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RPL7L1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M661"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050406.1">
        <property type="protein sequence ID" value="ENSPNAP00000082571.1"/>
        <property type="gene ID" value="ENSPNAG00000035825.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271870"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057667">
        <property type="entry name" value="HTH_SB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25787">
        <property type="entry name" value="HTH_SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sleeping Beauty transposase HTH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="68"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="29630" checksum="C03CBB042C70E578" modified="2024-11-27" version="1">MKSKELSVDLRDKIVSRHKSGEGYKKITAALKVPMSTVASIIRKWKKFGTTRTLPRAGRPSKLSDRGRRALVREVTKNPMITLSELQRSSVERGEPCRRTTISAAIHQSGLYGRVARRKPLLSKRHKAARLEFAKRHLKDSQTMRNKILWSDETKIELFGVNARRHVWRKPGTAHHEANTIPTVKHGGGSIMLWGCFSAAGTGRLVRIEGKMNAAMYRDILDKNLLQSALDLRLGRRFIFQQDNDPKHTAKISKQWLQDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNB5</accession>
    <name>A0A3B4BNB5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CLCA1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CLCR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNB5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001257"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012467.2">
        <property type="protein sequence ID" value="ENSPNAP00000001257.1"/>
        <property type="gene ID" value="ENSPNAG00000008061.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154682"/>
    <dbReference type="OMA" id="RQFTECE"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004727">
        <property type="entry name" value="CLCA_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013642">
        <property type="entry name" value="CLCA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00868">
        <property type="entry name" value="hCaCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF172">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR 4 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08434">
        <property type="entry name" value="CLCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0868">Chloride</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017284607" description="VWFA domain-containing protein" evidence="12">
        <location>
            <begin position="21"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="871"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="13">
        <location>
            <begin position="301"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="909" mass="99786" checksum="F9536B7614CCC422" modified="2018-12-05" version="1">MAKAFAVMLLLFNALGLNTGIKLDGNGYTDILVAINPAVPQNENIINQIKAMITSGSEYLLMAMDNKVFIKDVKILVPPNWSGSYEKARRETYDKANIIIDSPHPAYRDEPYVKQMKGCGEESEYIHFTPNFLLNDNLLNAYGPRGKVFVHEWAHLRWGVFDEYNEKEPFYFSQNQQIQPTRCSTQVSGHWFEIINGHATLCRYDDTWLPTKECQFFPDKDQKTSASVMYMPSLDSVTAFCHEGDHNIEAPNTQNQKCDNKATRTVIFQHSVDKDALQTLKPLQSAPPAPKFTVIQRRSRVVCLILDVSGSMQGQRILRLQQAATIFLREIVEEGAYVGIVKFSTSADIISSLTKIEGYARESLISKLPDTASGSTNMCTGLKLGFEVLQQDDKSAVGDDVIFLTDGEATDDIQSCLKSAVNSGAIINTIAMGLSASNVLVTMANQTDGKFIVADENLISNQLVDAFSSFTMSDGDLTKLTIQLVSTGSSTTGWFNGTVPIDRTVGNSTTFTIIYERSAPTVSIHAPSGSAYDQTRMTHNAATKTLTLNIPGTAEPGDWRYSFFNTESTAQAMTLTITSRAARDDVPPVMVKAQMNQLASDGSKPMVVFAEVSQNHVPVLGAKVMATLQSDTGYSEDLFLLDNGAGADNFKNDGIYSRYFAKLISGRYSLKVRVIPQEGAIRGSHNRNSGALYVPGYIVDGKVQLNPQKPPVSVQSADVGRFIRTATGESFVVNVPKGVTTKFPPAKITDLRAKIQVDTIFLSWTAPGEDFDFGTVESYDIRWSKHLKTILYNFRNATPVNTSALHPQEATLDEQFSFLPEVAIKNGTTLFFAIQSQDKQALKSEVSNIAQVTKFVPSPKPPEISEPPLKLIISIVTIACAVAILACIIATITVWALKRKKAVKESGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNZ2</accession>
    <name>A0A3B4BNZ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNZ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001293"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012409.2">
        <property type="protein sequence ID" value="ENSPNAP00000001293.2"/>
        <property type="gene ID" value="ENSPNAG00000008086.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155932"/>
    <dbReference type="OMA" id="CNNKDTN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000780">
        <property type="entry name" value="myc-associated zinc finger protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000095">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000108">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000445">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001404">
        <property type="entry name" value="vascular endothelial zinc finger 1-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="285"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001293.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="472" mass="50406" checksum="6B37D8AD5E780416" modified="2024-11-27" version="2">WMSRSYSCEQANEALHHQHQVGQNSLLPLLNSGAEPQDQKPVLPIPLDQKPPVSAAELLKDNVASGVGGGGGGGGGPGGGGPVVVKKEPKSKTPFICGYCNKAFRDSYHLRRHESCHTGIKMVSRPKKTTTAPTMVPLISTVPRDNNGNPSYVSTVAGILTTATTSASTAVGVMSALPQQQQQPSVPKKPPKPVKKNHGCEMCGKAFRDVYHLNRHKLSHSDEKPFECPICHQRFKRKDRMTYHVRSHDGGVHKPYICSVCGKGFSRPDHLSCHVKHVHSTERPFKCQVTACTSAFATKDRLRSHMIRHEGKVTCNICGKMLSAAYITSHLKTHGQASFTSPCNKGTPALAPNSSSTNTNNVCNSASATPVTASAVASTTAMNRGTVSNPVTIASNTVNITAPVSLQHPVTITAPVNIASVNIPATAPMNIAHPVAITTPMSMNITGPLNIAMRPMESMPFLSQVLPSSPPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQA4</accession>
    <name>A0A3B4BQA4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phospholipase A2, group IVC (cytosolic, calcium-independent)</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQA4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000609"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013564.2">
        <property type="protein sequence ID" value="ENSPNAP00000000609.2"/>
        <property type="gene ID" value="ENSPNAG00000030882.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234606"/>
    <dbReference type="OMA" id="WEWGTTR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF39">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2 3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="4">
        <location>
            <begin position="5"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000609.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="439" mass="49788" checksum="01AF8B07B05A72AA" modified="2024-11-27" version="2">MKPLLCSLFSSEVRIGHSLSEAERQHLARRRETILQCLEQNGISCSEVPNIAVLGSGGGLRAMVGLLGSLCQLKEEGLLDCIMYLCGVSGSTWCMASLYKEPDWSTKLDLVKNDIVQRLADGSVSLKEMGQKLAKYYSEKDHFSLTDVWSALIVSNMVNEIDEHKLTEQRGKYTKDPYPIYTVIDKQCKYDRLNADPWFEITPDESGYSLSGAFVDSSFLGSEFENGKNIKKQPEIDMLYLQGNSHQDENKMIKHWIAILRSIEKLVRWTWGTNNNYLYKMKVESADSSVSQSKTREYEDSGLLLNSPYFSVLRKEQDIDLIISLDFSEGDPFKTVVQTAEKCKDLQIPFPEVVLPAKNTEPQDFYVFKGSSDAPTVIHIPLFNAVNCKGKYMSKWVKTYSTFQMNYSREMITNLLEKAGLNIKNNKEKLLKEIGNVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BR87</accession>
    <name>A0A3B4BR87_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 21">Phosphatidate cytidylyltransferase, mitochondrial</fullName>
            <ecNumber evidence="7 21">2.7.7.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19 21">CDP-diacylglycerol synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20 21">Mitochondrial translocator assembly and maintenance protein 41 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TAMM41</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 21">Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24 25 26">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 21">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 21">Mitochondrion inner membrane</location>
            <topology evidence="3 21">Peripheral membrane protein</topology>
            <orientation evidence="3 21">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 21">Belongs to the TAM41 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="7 21"/>
    <dbReference type="RefSeq" id="XP_017560195.1">
        <property type="nucleotide sequence ID" value="XM_017704706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BR87"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002227"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010803.2">
        <property type="protein sequence ID" value="ENSPNAP00000002227.1"/>
        <property type="gene ID" value="ENSPNAG00000001140.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431503"/>
    <dbReference type="CTD" id="132001"/>
    <dbReference type="GeneTree" id="ENSGT00390000000616"/>
    <dbReference type="OMA" id="HAENMHR"/>
    <dbReference type="OrthoDB" id="341477at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015222">
        <property type="entry name" value="Tam41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619:SF0">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09139">
        <property type="entry name" value="Tam41_Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028840">
        <property type="entry name" value="Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 21" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 21" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17 21" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="18 21" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 21" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR028840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="338" mass="38101" checksum="C1C5CEA383D1E34A" modified="2018-12-05" version="1">MTLPALQNTSVFFRRILSQFPQDISLAFAYGSAVFKQAGTAQGQMGKNMLDFVFAVDDPVTWHTMNLIENRKHYSFLKYLGPKQISSIQNDYGAGVYFNTLVPSDERLIKYGVISTDALIDDLLHWKTMYVAGRLHKPVRILLQSENGKLRAALVANLKSAVNASFLMLPESFSEEDLFLQIAGLSYSGDFRMVIGEDKSKVSNIVKDNMQHFRILYNNILQDCPQVVYKPQQGRLEVDKSPEGQFTQLMALPRTLQQQITRLVDPPGKNRDVEEILLQVAQDPDCGLVVQQGIYSIVKTSSISQSAKGIITAGLLKSVSYSTKKLRKMWKGWRRKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSL1</accession>
    <name>A0A3B4BSL1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with PI4KB. This binding competes with FREQ/NCS1 binding in a calcium-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network membrane</location>
            <topology evidence="12">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017576641.1">
        <property type="nucleotide sequence ID" value="XM_017721152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BSL1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001510"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012051.2">
        <property type="protein sequence ID" value="ENSPNAP00000001510.1"/>
        <property type="gene ID" value="ENSPNAG00000000776.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441564"/>
    <dbReference type="CTD" id="556082"/>
    <dbReference type="GeneTree" id="ENSGT00940000159368"/>
    <dbReference type="OMA" id="MESSECQ"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
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        <property type="component" value="Chromosome 23"/>
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        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium-binding protein 8"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ca-binding_regulatory"/>
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    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
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    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46311:SF2">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46311">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004521"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="213" mass="24420" checksum="2232FCAEC0DBB3EE" modified="2018-12-05" version="1">MPVRAVTSRFMYRGLCSIPDILSYRTPVTLPEDEVEEIREAFRVFDRDGNGFISKQELGIAMRSLGYMPNEVELEVIIKRLDMDGDGQVDFEEFVTLLGPKLTASSMPDKFHGADFDSIFWKCDMQKLTVDELKRLLYDTFCDHLTMKDIENIIMTEESHMNSPECQVDIDTSPMQQVKHTCVRKSLICAFAIAFIISVMLIAANQMLRRGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTR5</accession>
    <name>A0A3B4BTR5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Myelin transcription factor 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYT1 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000009445.2">
        <property type="protein sequence ID" value="ENSPNAP00000003023.1"/>
        <property type="gene ID" value="ENSPNAG00000009411.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156364"/>
    <dbReference type="OrthoDB" id="10069059at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000001">
        <property type="entry name" value="Myelin transcription factor 1-like, a"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013681">
        <property type="entry name" value="Myelin_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF10">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08474">
        <property type="entry name" value="MYT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myelin transcription factor 1" evidence="14">
        <location>
            <begin position="728"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="173"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="684"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="773"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="845"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1188"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="403"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="456"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="479"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="511"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="693"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="786"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="798"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="882"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1310" mass="146569" checksum="5BAA4B6614ADE88A" modified="2018-12-05" version="1">MYGSAVLCVNRNTEVAYMSAPACFIKSLIPSSLQKPLLDFTGCCLLYDFSFIAELRSPCSLGSCARLRWWWNLLMCFVEIVLSYSNAVFLSEILLCVCVRKVCVSRAEAALFTHSCLTLRGQAMADLDLSLLNHAFRLDTVRPVLSSLLRLHTQNEYRGELLNQRWTRGDKISRCRPSLRPHSQPVKMSLDIDDKRTRTRSKGGRTPTEIVGQELSTLSCPVARKRRLQEEEEVEQQQQPVSKRKSHPLKLAMDDCFNAESDGSGEEMEMKEEDEEEDAETQQQCQPQEEEEQKPESNDADEADEEECMIIEASSTEKPKATGTDDLSSYQHIVANSLLHLSNRNNHATSPRQQQQQSVTMETPEDSPTRAEEEEEEGREKEAAKPTGQMNGVDGETEEEEERMQNGRQEESDHQYFTEEINVHLCEHHGKPHTEETYKEEYEDDEVEEQKKERNSLFNPSVITAAQGSHMNEDYITHKPSSPENYTSSKASVAPVFIEVRSEGSEKDDKDGDDDDDDEEEDNDSLSQRSTVADESEMYDMMRGNLGLLEQAIALKAQQVKGHREFPHTPEHHRYFHLDDRPIKHMEQLRKSYYGKESSRPEKRENKCPTPGCDGTGHVTGLYPHHRSLSGCPHKDRIPPEILAMHENVLKCPTPGCTGQGHVNSNRNTHRSLSGCPIAAAEKLSKSHDKQHLSQPMSEQPKGSPNSDRVLRPMCFVKQLEVPQFGSYRASLVPSTPRANLAKELEKYSKVSFDYASFDVQVFGKRMLAPKMHTSETSPKAFKSKPPFPRTSPPSPSMQSPYGKSSSSVHDYSHDAEAAHMAATAILNLSTRCWERPENLSTKHQDKNMDIEVDENGTLDLSMKKPIKREGGLLSTSPGVRSPDLSSTSSSSLHHGSSGISPHLQVYKQEEWEGPLDYTKPNRQREEELEEMDHTAQSFASSDPEDCDMGQDCPDDRKYPGEVTTPSFKVKFQPKECKKELLMCPTPGCDGSGHITGNYASHRSLSGCPLADKSLRSLMAAHTAELKCPTPGCDGSGHITGNYASHRSLSGCPRAKKGGIKTTPTKDDKEDSELLKCPVPGCDSLGHISGKYATHRSAYGCPLAARRQKEGLLNGTPFSWKAFKTEGPTCPTPGCDGSGHANGSFLTHRSLSGCPRASLAKKKAKFPGEEYLSSKFRASDVLDNDEDIKQLNKEITELSESNNEMEADMANLQTQISSMERNLKNIEQENKIIEEQNEALFMELSGLSQALIRSLANIRLPHMQEPITEQNFESYVSTLTDMYTNKDCYQNPENKALLETINQAVKGIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BU00</accession>
    <name>A0A3B4BU00_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MDM2 binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MTBP</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BU00"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009257.2">
        <property type="protein sequence ID" value="ENSPNAP00000003127.1"/>
        <property type="gene ID" value="ENSPNAG00000009534.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413984"/>
    <dbReference type="GeneTree" id="ENSGT00390000003305"/>
    <dbReference type="OMA" id="HFYGEQI"/>
    <dbReference type="OrthoDB" id="8633268at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031396">
        <property type="term" value="P:regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039061">
        <property type="entry name" value="MTBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029418">
        <property type="entry name" value="MTBP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029420">
        <property type="entry name" value="MTBP_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029421">
        <property type="entry name" value="MTBP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14382">
        <property type="entry name" value="MDM2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14382:SF1">
        <property type="entry name" value="MDM2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14920">
        <property type="entry name" value="MTBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14919">
        <property type="entry name" value="MTBP_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14918">
        <property type="entry name" value="MTBP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="3">
        <location>
            <begin position="263"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="MDN2-binding protein C-terminal" evidence="4">
        <location>
            <begin position="599"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="664"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="712"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="736"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="855" mass="95026" checksum="EBEBAAFA58E1C51F" modified="2018-12-05" version="1">MDRYLLVVVVNPLPKKEGSSGGFETARRLYARLEEISSSGCSSQVSPFPACSLSGSPAVPKCCFAIQASYGSAQFCSVEWEEVCVQRGDSEETVSSAVDGCISAVQEQEEQNLQAETSTLTEMFEEAAEALHQLADKLPPPGRVLLDVMVLCAEEAGLKEFLPVIGSLKHMQAWHSAHVNIVTEHSAGWSTAASYLSARVCSPAAVLDCVDERELWRGALLIRERKFVSELLFEGFSLKVQSRSSWRRMLHPHCSADHKLHAEVFQYYQPVLELLQLVKITDFPLLLQSSTEFELSLCSRSTKARLLLDQLRTLRGEVAALLSLSCVISAEAFPPASQLSASKWRHFMAKRPKVLSAPDVEVKGEKGRYLLLVQGAESGGCRARLIHSANQINGAVAMATLNSLIREQDLSSSGSARTSDWLRSLPCLRGDQLLRQERSLDRVQAQVLQECVRRRQQAQKPAAVPLNDLKVLLNLAREQYLNMQNSTLPRASERDSTKKDNHTSATRNVQPVIHSEWPERSVLRNHENMQKVRHRSRSSLFTTGSSECLMGPKDGQKSSSAQLDARELLKHFTPDGLPAGELQPLPVIRGGNTFQLSSELTPRKVAKLPFTQAASSHYHGIEFCLDERKALERDRGFVRLQSRLIRFETQTTCCKEPCPLPLALSPASTPSPAAPSEDGESLQSDPPQLKRRSREPDTHTHQHKRLVKSESSESVGSQCSVGSIPHPAVKALRQQAGRSLSTSSPATRTHSRTHATQKHSQAEPRPQTETCPQTESRSQKHNRMLKEVVLKTLKHHGISSEHECFEACSRRLFDVSKLYLKDLKTSRGLHEEMKKAAGRNAKQVIDWVVERNSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUR3</accession>
    <name>A0A3B4BUR3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MORN5</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUR3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003378"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008821.2">
        <property type="protein sequence ID" value="ENSPNAP00000003378.2"/>
        <property type="gene ID" value="ENSPNAG00000009801.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01070000254026"/>
    <dbReference type="OMA" id="DTGRMGH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31294">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31294:SF8">
        <property type="entry name" value="KERATIN-ASSOCIATED PROTEIN 21-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003378.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="136" mass="15218" checksum="501210BC96140606" modified="2024-11-27" version="2">EGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTEGYTSTQATFSRATQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BX48</accession>
    <name>A0A3B4BX48_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">TRIT1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A37). Mediates modification of a limited subset of tRNAs: tRNA(Ser)(AGA), tRNA(Ser)(CGA), tRNA(Ser)(UGA), as well as partial modification of the selenocysteine tRNA(Ser)(UCA). TRIT1 is therefore required for selenoprotein expression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BX48"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003095"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009311.2">
        <property type="protein sequence ID" value="ENSPNAP00000003095.1"/>
        <property type="gene ID" value="ENSPNAG00000009466.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015214"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.140:FF:000002">
        <property type="entry name" value="tRNA dimethylallyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF89">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="8 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017276219" description="tRNA dimethylallyltransferase" evidence="21">
        <location>
            <begin position="17"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger double-stranded RNA binding" evidence="22">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF12171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="530" mass="60065" checksum="9A0FDF77FF45C797" modified="2018-12-05" version="1">MLRAVWKLCAVSVMAAATVAVQRALGKAVIPPLVVILGATGTGKSKLAIELGKRVQAEIISADSMQVYKGLDIATNKVTSEEQAQCPHHMISFVDPLVSSYTVVDFRNRVLSLIEDMHRREKLPVIVGGTNYYIESILWKVLVDTGQENGTDLKAKLQKLGGPELHRQLMEVDPEMAAILHPHDVRKIARSLQVYQETGVKHSRLLEEQREQDGADGLGGPLRFSDPCIFWLHSNMDVLDKRLDDRVDDMVSLGLIEELKDFHQRFNEKKIQENSQDYQHGIFQSIGFKEFHEYLTAGADLSQEEREELKMKGIEALKQAVRRYARKQNKWVRNRFLKRPGTNIPPVYGLDVTDVARWEQMVLNPALGILDCLQKGEQPSVEPMRIEGEGPRNKRSHHMCELCDKIIIGDLQWTTHLKSKNHKYHVRKKRKVEESSVPHQDDASDLLPIPKCPKQGCQRLTLTSARVFALLCTCQRTRCSSAYLETSQKCGCWQSPVKHESSLTQGFYSLQRWAAAAQPPSLYPLCITFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0D5</accession>
    <name>A0A3B4C0D5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561776.1">
        <property type="nucleotide sequence ID" value="XM_017706287.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0D5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005238"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005639.2">
        <property type="protein sequence ID" value="ENSPNAP00000005238.1"/>
        <property type="gene ID" value="ENSPNAG00000001767.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432463"/>
    <dbReference type="CTD" id="101883139"/>
    <dbReference type="GeneTree" id="ENSGT00940000159218"/>
    <dbReference type="OMA" id="RCKPFNA"/>
    <dbReference type="OrthoDB" id="3214149at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000036">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000048">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000453">
        <property type="entry name" value="GLIS family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN GLIS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="266"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="359"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="615" mass="67719" checksum="A10230D05F8E4307" modified="2018-12-05" version="1">MGPTRFGPAALQLDKCGFSNSIYEEEQVPQHTLTSSSQCCFSRSFHTQSVNLCNVSKATVPIITADGLDLASVVYSTQRAIMYQCVSEVQSDCAHSQVANTSSSSSASLTPSFSSSSCCIGPELPGSRLVSCPTAIQGVLDDAAQQEVCLFPKNLKQEPGGTACSLRCENKDALFHLKYGHSYLCTSPNTANELKVSPAAPSEGLHAKLHCQEDLGGVPGGKEENSFLGNEQPCHWMDCRASYEQKEELVRHIEKVHVDQRKGEDFTCFWAGCVRRCKPFNARYKLLIHMRVHSGEKPNRCMFEGCSKAFSRLENLKIHLRSHTGEKPYVCQYHGCLKAFSNSSDRAKHQRTHLDTKPYACQLPGCTKRYTDPSSLRKHVKGHSTKTQHSQDQQQLCLEAEPEGMEDRLTTSSVHLLHASDQMEPCSLYELQQSSLTAVLSENSNSSRSPAVSGSTALFQTSRNSSTELSFPQNLPDPLHNNKITQNRLTCPLLYGSSSTSRPEDQQPAKETPFISTAQRPDLLHHERPVLQQPFHSFPAPPEQSYAEGGDNLHSCELNGYTFTPNFTPAADFSSSPDPQTVSGHFSSCPDEGLPMQMGGYDRCVGQICSLYAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2S3</accession>
    <name>A0A3B4C2S3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BSDC1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16019">
        <property type="entry name" value="SYNAPSE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="167"/>
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        </location>
    </feature>
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            <begin position="231"/>
            <end position="416"/>
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            <begin position="242"/>
            <end position="251"/>
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    </feature>
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            <begin position="287"/>
            <end position="306"/>
        </location>
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        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
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        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
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            <begin position="385"/>
            <end position="404"/>
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    </feature>
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        <location>
            <begin position="405"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005141.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="444" mass="49329" checksum="EDE8637A9896331C" modified="2024-11-27" version="2">MHATLSVNHREGGWWGGWLQHSFQAIKDRSSEAYEFLKRDLTEFSNVVQHDTTCSIVATASAVRNKLAVWLMCFLIDYRFMTKYVEGSSETTEKVKKSISNILGVITDTLAPPPDKTIDCDVITLVATPAGTTEVYDSSKARLYSLQADPATYCNEPDGPPEQFDTWLSSFSLEEKKAEISELLVNSPSIRALYTKMVPAAVAHSEFWQRYFYKVFQLDQEEARRVALKRRAEETTQSESLGWEEDEEDEFLGATSTSRLDFTPPLEDSQLPTVTIGAPCEPAVSPVDAASDVTPSVSSDSVSLPTQLENNPEQPVSTSRPAPAEEVTLKLSEARLEGAEEKQAGPEQTSSLEAERDQTPAENGTAKAEQTREEGPQDLRVFELNSDSGKSTPSNNGKKGSSTDVSEDWEKDFDLDMTEEEVQMALSKVDATAEFEDEDWENWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5S0</accession>
    <name>A0A3B4C5S0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IRS-type PTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017561741.1">
        <property type="nucleotide sequence ID" value="XM_017706252.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000006430"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003583.2">
        <property type="protein sequence ID" value="ENSPNAP00000006430.1"/>
        <property type="gene ID" value="ENSPNAG00000005483.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432434"/>
    <dbReference type="CTD" id="79930"/>
    <dbReference type="GeneTree" id="ENSGT00940000155980"/>
    <dbReference type="OMA" id="NDLYSTW"/>
    <dbReference type="OrthoDB" id="6243387at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of MAPK cascade"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
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        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF58">
        <property type="entry name" value="DOCKING PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="3">
        <location>
            <begin position="146"/>
            <end position="250"/>
        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
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        </location>
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            <end position="398"/>
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    </feature>
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            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51064"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="57163" checksum="0061EF849B9E57B2" modified="2018-12-05" version="1">MDVVAKEGSLFIQGVKFGKRIWRKTWVILFEPSPTGIGRLEMYDMRDGGSPPKPVGLKKLEKRVVRLADCLSITPAPGESCPTECAAFYLNTAERTYTLAGPKQDEWVPTLCQLAFQRNEGGSDSKRPQQNNDMSMSNNDLYSTWSPGQYQVKVLSSEASVRCCMSGSYLLSPEKDALCLLDLNTGKTAFYWPYRFLRRFGQIKDGITIEAGRRCHTGEGHFTFMSKQGNQIYRAIEEAIMHQSVQDLLSKATSQMQDSTTQQPPPRPLIDDRMKGCQGSPTRGMKDRPCLPLSRRNLALPAPPVPKALPPPPAPPAPPALPAVCTKPLVILSKPPAMSPKPPADTTKEVLYATIKPQPKPRVKTAPPKIPQPPPGPNYSPPEPKQKREEEKETKERYICGSDEDPDSDYCNLRQVVEVQQDSPDTDTIYSLVVPPDRQENKCSMVASQDQTTPMQNSLISSSEIPLDFRQVLSNVLFKDLTRMTPRSYGGSSERLAKLDVEMDDVDYCEIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7A3</accession>
    <name>A0A3B4C7A3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C7A3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006776"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002970.2">
        <property type="protein sequence ID" value="ENSPNAP00000006776.1"/>
        <property type="gene ID" value="ENSPNAG00000012741.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234592"/>
    <dbReference type="OMA" id="TIFYKGC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
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        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
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        <property type="entry name" value="ENDOD1"/>
    </dbReference>
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        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
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        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN ENDOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472:SF16">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017322845" evidence="1">
        <location>
            <begin position="23"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="3">
        <location>
            <begin position="58"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="2">
        <location>
            <begin position="59"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="299" mass="33993" checksum="6688DCD1AF01DB0D" modified="2018-12-05" version="1">MAPVGIPLFTLLLLAGLRVSGATVSDSFKECSQFLYMRTAPTGIRGGNLKKICQRYGDKPRYATLYDSSRRLALYSAYTFKKSDGQRRMDTPWMYEPQLASEEESGNMQTLPPSDKTDPLIEDSQAMLEDYVDAVEYERGPLNPDQHQADNQDKAATYTLTNVVPQITDFVEGSWTAYTDRIRRRLNNFCRGVSYVVTGVTISGLTIRRGGQDRMAIPKHLWSAYCCPRYDRNSPYEVRYMFPSYAAYGLNEIVGHSVVEVSLKTLESFLKNQTGMDKNVSIFLNGCVSENKWKKRKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9U9</accession>
    <name>A0A3B4C9U9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PPP1R27</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9U9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007189"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002235.2">
        <property type="protein sequence ID" value="ENSPNAP00000007189.1"/>
        <property type="gene ID" value="ENSPNAG00000013074.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159603"/>
    <dbReference type="OMA" id="YHTPTYY"/>
    <dbReference type="OrthoDB" id="71307at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053080">
        <property type="entry name" value="PP1_regulatory_subunit_27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46899:SF2">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 27-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="228" mass="25838" checksum="46D9D376D910F937" modified="2018-12-05" version="1">MKYSYQYPVSQYTRHSTYTPTTHYMPTLSTTKYYATSSYTPSYYSSTKYSPSHYTPPAYTPAYKSTSTYTSVFSKPTRQSSSHRTPVQQTPTAAVKPARTVRFTNDILFQDHVRHGELEMIGRFMRARKVRVDTIFHSGMAALHEAVLSGNLECVKLLIKYGADVHQRDENGWTPLHMACSDGYPEIARYLLSLGASMEAENENGEKPADLIDPECKELIKLFEVGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEM2</accession>
    <name>A0A3B4CEM2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Collagen alpha-2(VI) chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">COL6A2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Collagen VI acts as a cell-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type VI collagen family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554961.1">
        <property type="nucleotide sequence ID" value="XM_017699472.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEM2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009750"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016183.2">
        <property type="protein sequence ID" value="ENSPNAP00000009750.1"/>
        <property type="gene ID" value="ENSPNAG00000015213.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428453"/>
    <dbReference type="CTD" id="1292"/>
    <dbReference type="GeneTree" id="ENSGT00940000155682"/>
    <dbReference type="OMA" id="LIFRPME"/>
    <dbReference type="OrthoDB" id="9944853at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055001">
        <property type="term" value="P:muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000027">
        <property type="entry name" value="collagen alpha-2(VI) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000052">
        <property type="entry name" value="collagen alpha-2(VI) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000026">
        <property type="entry name" value="Collagen, type VI, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF29">
        <property type="entry name" value="COLLAGEN ALPHA-2(VI) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017446137" description="Collagen alpha-2(VI) chain" evidence="14">
        <location>
            <begin position="20"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="33"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="605"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="824"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="247"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1011" mass="107078" checksum="05FE341D6717ACB1" modified="2018-12-05" version="1">MMLTKVIALCFLYGTAVYGQVPACHEKPECPIDLYFLIDTSETIALQENPPGSLVESIKDFTIAFAQKLEDQDYRGLVKISWSVGGLHFSMTQQTISTLTNKNEFIQRVRDIKYLGKGTFIDCAITNMTKAMLSAPSKPNAKRFAVVITDGHVTGSPCGRIKVAAERARDELIKMFVVASSKNLDETALREIASSPAGVYRSNYMAVNLTGARPIILTSTIDRIYDAMLHLAYQECYSLRCLETPGPAGPPGHRGQKGAKGDNGEPGLKGDRGRQGDPGIEGPIGHPGSKGEPGYKGEKGEIGTQGRKGVAGLSGRNGTDGQKGKFGRIGAPGCKGDQGDKGPDGYPGDVGDTGLPGGAGEKGDPGRPGRPGPLGPNGDPGPKGERGSPGTPGLPGQKGSAGTPGGPGPNGDSGRRGDFGPKGSQGPPGQKGDKGEPGSEGLRGLPGEVGNKGTKGDPGLPGPRGPPGAPGEPGRNGSRGDPGDSGFRGDPGPPGPKGDPGRVGFSYPGARGPTGDRGDKGRPGPRGSRGDCGQKGEPGQKGPPGEPGEAGPQGEPGPRGPRGDPGRDGDPGPEGDAGLTDCDIMNYIRETCGCCDCEKRCGALDIVFVIDSSESVGLTNFTLEKNFVINTINRLGSIAKDPSSETGTRVGVVQYSHSGTFQAIRLNDSKIDSLSAFKDAVKQLEWIAGGTWTPSALKFAYDNLIRDSRRAKAKVTVVVITDGRFDPRDDDSLLTYLCTDSDIDVSAIGIGDMFEQQEENESLKSIACQKDGRVMGMRRFADLVAEEFIDRIETVLCPDPVIVCPDLPCKSEPAVAGCIQRPVDLVFLLDGSERMGQENFRRAREFVEKVAQRLTLARSDRDDRNTRVALLQYGDENSQEVAFQLTHNFTIISDGLAKIRYLDSASNVGSAIIYAVNNILMSGTSRLARRNAEISFVFITDGVTSNKNLEEGISAMRRMEGVPTVIAMGSDVDQEVLTKLALGDQSAVFKGQDFTHLFTPSFFERFIRWIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFH2</accession>
    <name>A0A3B4CFH2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFH2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010055"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016612.2">
        <property type="protein sequence ID" value="ENSPNAP00000010055.2"/>
        <property type="gene ID" value="ENSPNAG00000003399.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163891"/>
    <dbReference type="OMA" id="NDAARWY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07699">
        <property type="entry name" value="IgC1_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000283">
        <property type="entry name" value="Immunoglobulin kappa constant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256:SF65">
        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="16"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="140"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010055.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="242" mass="26305" checksum="F34490EE1D3A6BEB" modified="2024-11-27" version="2">KAEMLEFLNKSVSGVTVVTQKPPVLTPTKGETTTMHCNLGTVTSSAARWYKQVPGEAPQYVLVNYHGLSSPEYGSGFSSPKFTSTHSSQSDYTLIISNVEVGDSAVYYCSTWDSSSVLYLFFYGEQVWRCAGRGWYSAVPADVLTIFPPSSEELKSNKATLVCLASDMFSAFADVHWLLGGKAVSSGVTTGSAAQQANKKFRLSSYLTIERSEWDKDKAITCEVSAASKATTKEIKKSECSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CI34</accession>
    <name>A0A3B4CI34_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Solute carrier family 7 member 10b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017554790.1">
        <property type="nucleotide sequence ID" value="XM_017699301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CI34"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011015"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035949.2">
        <property type="protein sequence ID" value="ENSPNAP00000011015.1"/>
        <property type="gene ID" value="ENSPNAG00000016669.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428368"/>
    <dbReference type="CTD" id="559395"/>
    <dbReference type="GeneTree" id="ENSGT00940000156469"/>
    <dbReference type="OMA" id="PWRDVVP"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042941">
        <property type="term" value="P:D-alanine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042942">
        <property type="term" value="P:D-serine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000008">
        <property type="entry name" value="large neutral amino acids transporter small subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF73">
        <property type="entry name" value="ASC-TYPE AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="57499" checksum="70833ACB4386E1F3" modified="2018-12-05" version="1">MDGAERKRGVRARQEPQAPRSTMKDGKVKEDTVPDRVTLKKEIGLLSACTIIIGNIIGSGIFISPKGVLEHSGSVGLALVVWVLGGGIAALGSLCYAELGVTIPKSGGDYSYVTEIFGGLVGFLLLWSAVLIMYPTTLAVIALTFSNYVLQPAFPNCVPPYMATRMLSATCIIFLTWVNCSSVRLATRIQDIFTVGKLLALGLIIAVGLLQIYKGNYEGLTPQIAFEFYKTPSVGQIALAFLQASFAFSGWNFLNYVTEEVVDPRRNLPRAIYISIPLVTFVYTLTNIAYFSSMSPEELLSSNAVAVTFGEKLLGMFSTIMPISVALSTFGGINGYLFTSSRLCFSGAREGHLPSLLAMIHCKSCTPIPALLVCCTATIVIMCIGETHNLINYVSFINYLSYGVTIAGLLYYRWKKPNLYRPIKVSLLVPVCYLLFWALLLGFSLHSEPVVCGVGLVIMLTGVPVYFLGVHWKDKPKCIYDFIEWATYLGQRVFFVVFPHIDPTETATPTEWTDKSSITSKASGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIM0</accession>
    <name>A0A3B4CIM0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system. Acts by forming homophilic or heterophilic trans-dimers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Cis- and trans-homodimer. Can form trans-heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIM0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010344"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016957.2">
        <property type="protein sequence ID" value="ENSPNAP00000010344.2"/>
        <property type="gene ID" value="ENSPNAG00000015876.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156933"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902414">
        <property type="term" value="P:protein localization to cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000304">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF69">
        <property type="entry name" value="NECTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="34"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="143"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="243"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010344.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="484" mass="53789" checksum="05F18C7C113B0CDC" modified="2024-11-27" version="2">IYCYFCQFQMISVTIVVASGQMVKMDSDRSGYVGSQVELRCEFVNSNPPVKISQVTWQKFVNGTKQNVAIANPALGVSVLAPFKERVRFKNPAVRQRTPSLEDTTIIFNNLRLSDEAAYICEYTTFPAGNRESMVNLTVYARPMVQMSLSTPTIVAGSKDLKMTVATCISANGKPPSVITWDTELNGEANTEEIRNTDGTITVRSDYIVVPSRETHQQRLTCISTYNDEQYTDSVSLNIQYEPEVIIEGFDGNWYINRDNVQLTCLADANPAISLFQWRYLNGSMPNSVELRDDTLIFKGPVTYEIAGTYVCDATNSIGTGSASVEVTVSGKSAWHEQQRAGAAIGGAVVCGTVLLAAITLLAVFFHRQRRTFKGDYSTKKQVLGNGFGKAGSLPPCPSFPQSLNYTEDSNDEKKPEVYGGESVQEFHGYPDNRMKAYSTVEDSQRCRYDEQSYLYDYGSEVEISVDMVPQMDGSVISKEEWYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJE1</accession>
    <name>A0A3B4CJE1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Inositol-tetrakisphosphate 1-kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITPK1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJE1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012132"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019332.2">
        <property type="protein sequence ID" value="ENSPNAP00000012132.1"/>
        <property type="gene ID" value="ENSPNAG00000017900.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001278"/>
    <dbReference type="OMA" id="QHLYNRQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11370:FF:000001">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000047">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041429">
        <property type="entry name" value="ITPK1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF1">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17927">
        <property type="entry name" value="Ins134_P3_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Inositol-tetrakisphosphate 1-kinase N-terminal" evidence="11">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="10">
        <location>
            <begin position="120"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="325"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="45790" checksum="E9CE539828BB2255" modified="2018-12-05" version="1">MQTFLRGRRVGYWLSEKKMKKLNFLAFADMCRKRGIEVVQLDLSQPLEEQGPLDVIIHKLTDLILEADQNDTQSLLLVQRVQDYIDAHPETIVLDPLPAIRTLLDRCKSYQLVHRIEDCMQDERICSPPFMVLNSECGPDTLEQIKQHGLTFPFSKSLLFLSGKSQRKWQLMAIIFSAEDLKDIKPPCVIQSFINHNAVLYKVFVVGDSYTVVERPSLKNFPSGPADRKAIFFNSHNVSKPESSSDLTSRDNVEGVSQPPSDDVIRELSRSLRQALGVSLFGIDVIINNQTGQHAVIDINAFPGYEGVPEFFNDLLNHITSVLQGQSSNDCPTPPSAQPPSGQLTAPASGERSCAPSQECCSLLGKESDSSSPWIVEGEGGLKGSCQRLGCTPAMSPNFQQHCVSTIATKASSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CK04</accession>
    <name>A0A3B4CK04_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ANKS4B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017539701.1">
        <property type="nucleotide sequence ID" value="XM_017684212.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CK04"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010854"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017628.2">
        <property type="protein sequence ID" value="ENSPNAP00000010854.1"/>
        <property type="gene ID" value="ENSPNAG00000016461.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412259"/>
    <dbReference type="CTD" id="257629"/>
    <dbReference type="GeneTree" id="ENSGT00390000017548"/>
    <dbReference type="OMA" id="TQRNDQE"/>
    <dbReference type="OrthoDB" id="76949at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000034">
        <property type="entry name" value="ankyrin repeat and SAM domain-containing protein 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000074">
        <property type="entry name" value="Usher syndrome type-1G protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF109">
        <property type="entry name" value="ANKYRIN REPEAT AND SAM DOMAIN-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="30"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="7">
        <location>
            <begin position="353"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="430" mass="48207" checksum="5FBBA3CE124F4EBC" modified="2018-12-05" version="1">MSRYHKAAIDGYLDLLKEATKKDLNTPDEDGMTPTLWAAYHGRLEALQLICSRGGDPDRSDIWGNTPLHHAASNGHMHILSFLVNFGANLFALDNDFHTPMDVAASRDQMNCVRFLDNAASQQTHQNAKKVARLKEQAVKDAERRVKLCEKVKKRHQSKMDKMYRGGGSVSEASMSSLSSTGTMNNVNEQFSKLIASDTTGSIKATIKGTLQRKFGKKDKETVSRQGDSNVIYVKQENGNAEKPDFTDVFNELDENEDDEDGMGGFKDEKDEEGAGQVKESIFKRPGLGNMVFRKNFSMEMGIEPDEFPSGDTEDLGYLIRKEVFETEDEGLEDYSEEDSQLPWKQEELGLDEDEEETSPLDAFLNSVNLLDFAPVFVREQLDLEALMLCSDDDLKSIRIQLGPRKKILEAAARRKAALEQPGVIKDTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CKT6</accession>
    <name>A0A3B4CKT6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Occludin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell junction</location>
            <location evidence="12">Tight junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 15">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CKT6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011154"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018038.2">
        <property type="protein sequence ID" value="ENSPNAP00000011154.2"/>
        <property type="gene ID" value="ENSPNAG00000016801.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165052"/>
    <dbReference type="OMA" id="TEMDEYP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002958">
        <property type="entry name" value="Occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF6">
        <property type="entry name" value="OCCLUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005993">
        <property type="entry name" value="Occludin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01258">
        <property type="entry name" value="OCCLUDIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="19">
        <location>
            <begin position="58"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="OCEL" evidence="20">
        <location>
            <begin position="427"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="298"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR005993-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011154.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="535" mass="59804" checksum="0EC542ABC76B1A34" modified="2024-11-27" version="2">MFEKQPYDSPPMYTPPSNGFGPPGSFQPPRTEMDEYPPPPGSYFVGEEIPQNFYKWSSPPGIVKIMEGAIVVLCLGIFACVASTLVWDLQYGYGYGTGLGVGYGTGYGSGYGYGSYGYGGLGSGYYGSGYYNYPTPYTAKTAMIAMAAINFIVALGFFVASFSKTRAVRSRKFYLAILVCSIILAVLQGIINIVYIVGVNPMAQSSSSMLYNPMLMMCQSLYGNSVSGMGAGFPLYNQYLYHYCYVDPQEAVAMVCGFLVVIALAVAAFFSYKTRSKIWRYGKPNVYWDEPLTGGPEGRDVEDWVNNVDQDGQSTHEPSVMFSEKGAPPLNKSQSQSVSSIPPKSPGGFSSDPYTPPAYSDRLLGRSYEPEYPPGGASSSPSDRTDGVRKPSANRGKRRRRNPELDESQYETEYTTGGETGNELDQDYWMSMYPEISSDAQRQEYKREFDSDLRRYKQLCAEMDDINDDLNKLSRELDTLDEGTTKYEASNRSLNLLHITLRMPDYQEKKLQCRRLRHKLFHIKRMVKSYDRAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CL94</accession>
    <name>A0A3B4CL94_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CL94"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012128"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019320.2">
        <property type="protein sequence ID" value="ENSPNAP00000012128.2"/>
        <property type="gene ID" value="ENSPNAG00000017827.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162996"/>
    <dbReference type="OMA" id="VVNFLVW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000312">
        <property type="entry name" value="Alpha-2-macroglobulin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.20.50.160:FF:000002">
        <property type="entry name" value="Thioester-containing protein 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF150">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="9">
        <location>
            <begin position="447"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="10">
        <location>
            <begin position="718"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="11">
        <location>
            <begin position="1331"/>
            <end position="1420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012128.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1425" mass="159518" checksum="39222743223117D6" modified="2024-11-27" version="2">TKFIMVVCWMYISKRLLLVWFFFLCAYGRPSGPYFMVTFPAVIESGSEATLCTSLLKPNETLQMTIYLVHDNQNRTLLQETVEEEFRRCSKFKAPQVEGESVQEIRVDVKGESFEMTEKRRVMFKSYKPLTFIQTDKPIYNPGQTVNFRVVTMDLNFVPLKLEDNNGNRIGQWTNVSSTGLIVQLSHELNPEAPEGLYILEAYIGDRETRHYFKVKKYVLPRFEVTVKAPKQHSVGEELKIELCAKYTYGHPVPGKALVQVCRAFFTYSEEHASLISPCLDNTTGCASAVFNMSAFTNPEFQHIFKDNLDATITVTEEGMEISMVKTEEIQLIYEIGKTVFVDVPHNFERNTVIKGAVSLFFFSGRLIPNKKVYLLEGPLWSAKLLQNLTTDMNGLANFTINAPDHPHTQILLEASVYPDGRYDEYTPFFSTDDVNIHLLHPAPTIFRRLKEPIPCGTEITLNITYYIVGETTENYSTDIIYTVMSKGAIVHHGYEKVEVKESKKFIEGKVSFRLSVDVGLAPVVQVLVYCVLPSEKIITASKNFNVEKCFRNKVSLQFSPTEAVPGEENTLQLSADPGSLCGLSAVDQSVFIMEPGKRLNADQVFNLLPVSSVSHYPYSVVDRQECLHVRTRRYLWPDYVYTTIQRVGLKMVTNLAIRPPQCLMYKGMQYILYCNVLKYFQCMLIMNVYRSTDYDPLVGSSWLSDSTETIRKFFPETWIWQLVEVGDSGSAQVPVTVPDTITTWETDAFCLSSKGLGVAPPAKLRVFQPFFLELSLPYSIIRGEEFELKATVFSYLSKCIMVKVTPAPSSLYTLKASSDGEYSSCLCANGRKTFKWTLVPSVLGVLNVTVSAEAEQSQTVCDNEIVSVLERGRIDTVTRSLLVQAEGIEKTQSQSWLLCPQENSVSEEMELGLPEDVIKGSTRASVSVLGDILGRALKNIDGLLKMPYGCGEQNIALLAPNIYILQYLENTGQLIAAIRERATGFLKSGYQRQLNYKHDDGAYSTFGTGDGNTWLTAFVLKTFGKAQRYIFIDPQNIDSAKNWLLSNQGPDGCFIRQGRLFNNRMKGGVKDDVTMTAYITASLIELGHTVKDPIVSKGLLCLKSSIGNLTNTYTTALLAYTFSLAKEHDTREHLLKKLDSVAISGGGPLHWSQSASDDSDSLSVEISSYVLLAVLTTAEVTAADLGYANRIVTWLVRQQNPYGGFSSTQDTVVALQALALYSTKVFSSDGSSTVTVQSADKQTHHFDVNEDNKLLYQERPLQVVPGKYSILVKGSICVSVQMAFFYNIPTPAETSTLSIKATTEGRCSESLAKTLSVNFTVTYNGTLNSTNMVIVDIKILSGFTADSSAVSSSLKYMELVEQAETKDDHIIMYIKEVPRNIPMNYELHLKQVLPVKNLKPAVIKVYDYYQTSDQSETEYSSPCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQ52</accession>
    <name>A0A3B4CQ52_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Chloride channel, voltage-sensitive 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQ52"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014197"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037240.2">
        <property type="protein sequence ID" value="ENSPNAP00000014197.2"/>
        <property type="gene ID" value="ENSPNAG00000003638.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157383"/>
    <dbReference type="OMA" id="KHIGDPE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03683">
        <property type="entry name" value="ClC_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000003">
        <property type="entry name" value="Chloride channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000032">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000058">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF4">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="724"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="743"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014197.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="805" mass="89854" checksum="615BB8CF82D41D01" modified="2024-11-27" version="2">MYIVTKLGEDWLFLVLLGFTMALISWSMDYASAKSLQAYKWMYVELKGNVPLQYLAWVTYPIILIVFSSLFCHLVAPQAIGSGIPELKTILRGVVLKEYLTLKAFVAKVIGLTASLGSGMPLGKEGPFVHIASICAAVLSRVLSIFTGVYENPYGYTDILTVGCAVGVGCCFGTPLGGVLFSIEVTSTYFAVRNYWRGYFAATFSAFIFRVLSVWNKDSVTITALFRTNFRMEFPFDLQELPAFAVIGISCGFLGAFFVYLNRQVVLFMRRPTALTRFLTKHRLIYPAVVTLIIATFTFPPGFGQFMAGELMPRECINSLFDNFTWTKIWRSASPPGLGRSAMWLHPHVSVFIILLLFFIMKFWMSAVSTTMPIPSGAFMPVFILGAAFGRLVGEIMATLFPNGILFDGIVYRILPGGYAVIGAAAMTGAVTHTVSTAVICFELTGQISHILPMMVAVILANMVAQGLQPSLYDSIIQVKKLPYLPELGFGHISKYNIFVEDIMVRKVKFLSPQSTYRELDLLLQSTTLKTFPLVDSKESMILLGSIERCELQAACDWWLSAERRVYNQGQGISSPGSKVSWECFTFIDEEEQEENKEKVRPKWIHRGAAHSIVCSVATVYHCDITTDATFDWPLQIKAWEEAEMDKPIDIDHIRIDPSPFQLVERTSLHKTHTLFSLLGLSHAYVTSIGKLVGVVALKELQKAIEGSTGSGVRLRPPLASFRDANRKAKKHPHTPSAPSSPTRDRELWSEGGKRDVKDDTEAKLAVAKETEYKVTTERERQTDRQTDRQREGEKGESTLEEPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSQ7</accession>
    <name>A0A3B4CSQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547687.1">
        <property type="nucleotide sequence ID" value="XM_017692198.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CSQ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013781"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021483.2">
        <property type="protein sequence ID" value="ENSPNAP00000013781.1"/>
        <property type="gene ID" value="ENSPNAG00000019671.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424286"/>
    <dbReference type="CTD" id="353154"/>
    <dbReference type="GeneTree" id="ENSGT00940000160673"/>
    <dbReference type="OMA" id="KRFGCAE"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035118">
        <property type="term" value="P:embryonic pectoral fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048332">
        <property type="term" value="P:mesoderm morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032475">
        <property type="term" value="P:otolith formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045743">
        <property type="term" value="P:positive regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22541">
        <property type="entry name" value="SP5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000061">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF29">
        <property type="entry name" value="TRANSCRIPTION FACTOR SP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="263"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="341"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="369" mass="41161" checksum="098D388BDEF1AA91" modified="2018-12-05" version="1">MAAVAVLRNDTLQAFLQDRTPNSSPENSKHSPLALLAATCNRIGHHHGSNPAEFLQVPYETTLGSPSRIFHPWSNEANPPSNSSFGLSSKPQLTAHLQGSYTSHHELPLTPPADPTYPYDFSPVKMLPCSMQSTCPPTYVPAVTYAAPTPIPPAMPGFVAGHSGLVHQQQRQLSPNPGEDIPWWSLQQGNPVSHPSSLAPHRFPIQRSLVLGHTDFAQYQSQIAALLHTKSPLATARRCRRCRCPNCQSSSGSNEEPGKKKQHVCHIPGCGKVYGKTSHLKAHLRWHSGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFVCPDCCKRFMRSDHLAKHVKTHQNKKGKPHEKPAEHHVKREDTRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYF9</accession>
    <name>A0A3B4CYF9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAM50A/XAP5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FAM50A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYF9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024838.2">
        <property type="protein sequence ID" value="ENSPNAP00000016363.2"/>
        <property type="gene ID" value="ENSPNAG00000022565.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004735"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048337">
        <property type="entry name" value="FAM50A/XAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007005">
        <property type="entry name" value="XAP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722:SF0">
        <property type="entry name" value="PROTEIN FAM50A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722">
        <property type="entry name" value="XAP-5 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04921">
        <property type="entry name" value="XAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM50A/XAP5 C-terminal" evidence="2">
        <location>
            <begin position="212"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016363.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="358" mass="41805" checksum="4CF9CD49533F39AE" modified="2024-11-27" version="2">MNEGTNQTTGSGCSISAGGSAMAQYKGAASEAGRAMQLMKKREREREQLEQLKQKIAEDNMVKSNIDKKFSAHYDAVEQELKSSTVGLVTLNDMKAKQEALVKEREKQLAKKEQSKELQLKLEKQKEKKRKEEQKRKIASLSFNPDEGEDEEEEENDEEEEDDTPIKKKKLGKNPDVDTSFLPDREREEEENRLREELRQEWERKQEKIKGEEIEITFSYWDGSGHRKTVKMKKGNTIQQFLQRALEVLRKDFSELRSAGVEQLMYIKEDLIIPHHHSFYDFIVTKARGKSGPLFSFDVHDDIRLVNDATVEKDESHAGKVVLRSWYEKNKHIFPASRWEPYDPEKKWDKYTVSNFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ39</accession>
    <name>A0A3B4CZ39_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">L-type lectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LMAN1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ39"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017352"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026092.2">
        <property type="protein sequence ID" value="ENSPNAP00000017352.2"/>
        <property type="gene ID" value="ENSPNAG00000023629.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159146"/>
    <dbReference type="OMA" id="WSAEFQF"/>
    <dbReference type="OrthoDB" id="10265193at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06902">
        <property type="entry name" value="lectin_ERGIC-53_ERGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000028">
        <property type="entry name" value="Blast:Protein ERGIC-53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF28">
        <property type="entry name" value="LECTIN, MANNOSE BINDING 1 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043938210" description="L-type lectin-like domain-containing protein" evidence="9">
        <location>
            <begin position="24"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="10">
        <location>
            <begin position="31"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017352.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="517" mass="58081" checksum="426D1676C31F2F7D" modified="2024-11-27" version="2">MAARALVAAVVLCVCAELQEARGSGGDAPHRRFEYKHSFKGPHLSRDGSIPFWVHTGSAIPSADQIRITPSLRSQKGSVWTKTPVSFEHWEAEVAFRVSGRGRTGADGLAIWFSAAQGLEGPVYGAADQWNGVGIFFDSFDNDGKKNNPAVIVVGNNGKLVYDHQNDGSTQALGTCLRDFRNKPYPVRARITYYKKTLTVLINNGFTPEKEDYEFCTKVDNMIIPPTGYFGISAATGGLADDHDVLSFLLFRLTEPGQELPPPEQEIPKEEQDKYQEEFQNFQLELDKKKEEFQKEHPDVQGQPLEDLYESVSDREFRQVFEGQNRIHMEIKQLNRQLAMILDEQRRYVSVITEEISKRGTQSGQAPSQELDTVISTQQEVLRNLDDLRNTVSASVKQLAVGQHENVGGLGSYETVQHFNDIKEHLHVVKRNIEHIIQKNTGPAEKPKCPELPPLPACLTMAHFVAVVVIQTLLFLVDLFLKCACKCFVCFSLNMWEKLGLFTLEDVNIFSLKVNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZU9</accession>
    <name>A0A3B4CZU9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the dynein light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558333.1">
        <property type="nucleotide sequence ID" value="XM_017702844.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZU9"/>
    <dbReference type="SMR" id="A0A3B4CZU9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016099"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024483.2">
        <property type="protein sequence ID" value="ENSPNAP00000016099.2"/>
        <property type="gene ID" value="ENSPNAG00000033149.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430381"/>
    <dbReference type="CTD" id="8655"/>
    <dbReference type="GeneTree" id="ENSGT00390000000378"/>
    <dbReference type="OMA" id="YICCNVL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21452">
        <property type="entry name" value="DLC-like_DYNLL1_DYNLL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000001">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016099.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="89" mass="10360" checksum="448708C670438B3F" modified="2024-11-27" version="2">MSDRKAVIKNADMSEEMQQDAVECATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D1V3</accession>
    <name>A0A3B4D1V3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Neurturin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NRTN</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Growth factor that supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Involved in the development of the neural crest. Might control the size of non-neuronal cell population such as haemopoietic cells. Acts by binding to its coreceptor, GFRA2, leading to autophosphorylation and activation of the RET receptor. Heparan sulfate-binding is required for signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer; disulfide-linked. Interacts with GFRA2 coreceptor and RET: forms a 2:2:2 ternary complex composed of NRTN ligand, GFRA2 and RET receptor. Also forms a 4:4:4 tetrameric complex composed of 4 copies of NRTN ligand, GFRA2 and RET receptor, which prevents endocytosis of RET.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TGF-beta family. GDNF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569739.1">
        <property type="nucleotide sequence ID" value="XM_017714250.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D1V3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017545"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026376.2">
        <property type="protein sequence ID" value="ENSPNAP00000017545.1"/>
        <property type="gene ID" value="ENSPNAG00000023812.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437264"/>
    <dbReference type="CTD" id="4902"/>
    <dbReference type="GeneTree" id="ENSGT00950000182993"/>
    <dbReference type="OMA" id="YHTIHEV"/>
    <dbReference type="OrthoDB" id="9936891at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030116">
        <property type="term" value="F:glial cell-derived neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035860">
        <property type="term" value="P:glial cell-derived neurotrophic factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19383">
        <property type="entry name" value="TGF_beta_Neurturin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000037">
        <property type="entry name" value="neurturin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043401">
        <property type="entry name" value="GDNF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173">
        <property type="entry name" value="GDNF SUBFAMILY OF TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173:SF3">
        <property type="entry name" value="NEURTURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="12">
        <location>
            <begin position="124"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="232" mass="25746" checksum="3285BEF399E3743E" modified="2018-12-05" version="1">MKLWKCAAIALTLCGAALSVFLSRILLPLKAPVPLHFERSSFSSSSSSPPPLPSTASPASSMSSNSSSSWTGRKFRRVRSANGANSVISEFINMFQSFTEGELKQIIAALVERKAQRDAKQGKRTKRAKNGSKVCSLQEEEVTVSQLGLGYISDETVLFRYCSGRCEASRRNYDMTLAILKRNSKLIKEDLKKAKHRPCCRPTAYDKNISFLDNQSRYHTIHEVSARKCGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D386</accession>
    <name>A0A3B4D386_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fish-egg lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MPV17</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tectonin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D386"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028222.2">
        <property type="protein sequence ID" value="ENSPNAP00000018807.1"/>
        <property type="gene ID" value="ENSPNAG00000025266.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047886"/>
    <dbReference type="OMA" id="VWGVNTH"/>
    <dbReference type="OrthoDB" id="166585at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006624">
        <property type="entry name" value="Beta-propeller_rpt_TECPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051513">
        <property type="entry name" value="Tectonin_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250:SF3">
        <property type="entry name" value="FISH-EGG LECTIN-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19193">
        <property type="entry name" value="Tectonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00706">
        <property type="entry name" value="TECPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017232392" description="Fish-egg lectin" evidence="3">
        <location>
            <begin position="20"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="27556" checksum="303956B7746A0D07" modified="2018-12-05" version="1">MTRTAVLFLLLNLWITCLALQCTEVPGRLKQIDAGNGQVFGVCPKGDIFTLYGSSFIRLQGLLKHVSVGPAGVWGVNSTNQIHKLVKGDWVPVPGMLKQVDAGGKMFVAGVNPGGLVFCLGAEATTKYAGPGSPASWTQLPGKLKYYSCGPHSCWGVNVEEQVFLMKGVTPTACAGSLEWQQIPGALSMIEVSTDGKVYGLDSEGDVHQREGVAPCNLAGTGWRKINLSQKVRHVSYDLGYLWLVGRDNHITYCTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3L2</accession>
    <name>A0A3B4D3L2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3L2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018942"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028457.2">
        <property type="protein sequence ID" value="ENSPNAP00000018942.2"/>
        <property type="gene ID" value="ENSPNAG00000034251.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182977"/>
    <dbReference type="OMA" id="GDADIYW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF87">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="18"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018942.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="162" mass="18067" checksum="D328BFD7DD8885D4" modified="2024-11-27" version="2">MVPVPRSSVPIPCPVLAPVLVYNAELIGFCSADSESLKTLRNLTVRCGGSLTIPCFYDEKYKSNRKYWCKGQDWINCKIVAYTNTSGRTSLTDHPTQNMFTVELKRLQDSDSGYYWCAVEITGTGSDDGDYVYLMVSSDKTSLCGNEWSGRGQNQCPVFLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5M6</accession>
    <name>A0A3B4D5M6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosylation factor-like protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ARL11</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in apoptosis. May act as a tumor suppressor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554527.1">
        <property type="nucleotide sequence ID" value="XM_017699038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5M6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018753"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039116.2">
        <property type="protein sequence ID" value="ENSPNAP00000018753.1"/>
        <property type="gene ID" value="ENSPNAG00000025213.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428216"/>
    <dbReference type="CTD" id="115761"/>
    <dbReference type="GeneTree" id="ENSGT00940000166383"/>
    <dbReference type="OMA" id="GQAVETC"/>
    <dbReference type="OrthoDB" id="8484332at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000898">
        <property type="entry name" value="ADP-ribosylation factor-like protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="2 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="176" mass="19616" checksum="E6368E1E58082658" modified="2018-12-05" version="1">MGHMLSKKFLKTQQVVLMGLDSAGKSTLLYRIQRGVVMQTSPTVGFNVLTLELDKKTVFTVWDIGGQGSMRAYWKYYLENCEALVFVVDASDRARMEEAKTALKKVLSDHNMTDIPLMVLANKSDLPDAMTVSEVSKQLDMDSYKNGVWEIQACSALKGLGLQQAFQSVAKLIQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8I9</accession>
    <name>A0A3B4D8I9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Taperin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017569625.1">
        <property type="nucleotide sequence ID" value="XM_017714136.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8I9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040143.2">
        <property type="protein sequence ID" value="ENSPNAP00000020672.1"/>
        <property type="gene ID" value="ENSPNAG00000007100.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437194"/>
    <dbReference type="CTD" id="286262"/>
    <dbReference type="GeneTree" id="ENSGT00530000064035"/>
    <dbReference type="OMA" id="SKWQQNG"/>
    <dbReference type="OrthoDB" id="9945184at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025903">
        <property type="entry name" value="Phostensin/Taperin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025907">
        <property type="entry name" value="Phostensin/Taperin_PP1-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026671">
        <property type="entry name" value="PPP1R18/Tprn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21685:SF1">
        <property type="entry name" value="TAPERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21685">
        <property type="entry name" value="TON-B BOX DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13914">
        <property type="entry name" value="Phostensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13916">
        <property type="entry name" value="Phostensin_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phostensin/Taperin N-terminal" evidence="7">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Phostensin/Taperin N-terminal" evidence="7">
        <location>
            <begin position="91"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Phostensin/Taperin PP1-binding" evidence="6">
        <location>
            <begin position="631"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="255"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="481"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="576"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="646"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="747"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="603"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="672"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="792"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="823"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="853"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="868" mass="92370" checksum="45F23272AC3D1922" modified="2018-12-05" version="1">MSSGGDVGVLRQEESARMPAWKRELLERRKAKGGEATARSGSKSEDEATGTETRHHGASPATSPVKTKEPWSSPPSPSHTNGRATETDDDDGGGGGLPESLVLRESLGPLQENPFIKLEKERKKRRERENAARPLHHVLELYGSVPGIRTIRAENIIIIESDPDYFPEGAGLKHRAKPQQNGTVSGYSSLNDLLDRRSSPVTEIRAREVVVYDTALSRSEENLSTLGRPAGDLAELTEGQGRVSRMLQKFDRNYGKLQGKSRSSENLLDLDTGPSRFSPRPKAQPDFVPKYAASPQPSSPVRVSQSQVVAEKVFQNSQSSKTKLHDSPGESASSTPVSGSPQLVSSFRRRFEASGGRSVTVNPREEAERSQAKPVWEWDGMDGASKAKVPCSPESHHAHAESSPSPVAITELITSPSFEIRPSPRPDLSSLPASDLQARALANLRLQSRNSFTVIPKRPGSAPVPSSPVAPLAPSSPFAPLAPSSPVAPLAPSSPVAPLAPSSPVAPLAPSSPVAPLAPSSPVASLAPSSPVVSLAPSSPVLSVAPSSPILSPVPTVPTSASPTPLPAALSTEKLAKTPRPLSPAKVELPQPASPRLSLTNLSHNSTTPSPDSPTKSVSLPAPQEAQAPDRLPITNIDDVVVDPLPPFPSPMVQRRKGNTFTVVPKRRSEPQLASTEAQEPASTPQGSPSTPQAPYAQLGTLLKKRYPAVEEIEVIGGYLSLGRSCLSKTGSTGKKLTISFNESSLQSTFEYPSESSAWDSGEEEEEGGRAGDRGGEEDGGISMDRIRIPRPTYTSSPTNHTPNSTDLSNYTPKHSVEFTAWQEHKLDDPVHLGDGSSQQTNMSEEVMLTPADSSSLSDFSSEPALYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCZ8</accession>
    <name>A0A3B4DCZ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Orange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCZ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020941"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031848.2">
        <property type="protein sequence ID" value="ENSPNAP00000020941.2"/>
        <property type="gene ID" value="ENSPNAG00000027909.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287258"/>
    <dbReference type="OMA" id="SHIHMEM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Orange" evidence="5">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020941.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="135" mass="15642" checksum="191C2FC588628839" modified="2024-11-27" version="2">MTPAVPSAMTISKEHLPLNNKLRKPVVEKMCRDRINSSIEQLKILLTPDFLNQQPDHSKAVSQGFSRCVHEVLHFLCKEEMKTQSQRKLPNHFQNLQPSTEEKWRESDLPLLSSTDHLTISKEKSSVQSALWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDB6</accession>
    <name>A0A3B4DDB6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CXXC-type zinc finger protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with DVL1. Interacts with RBPJ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017581232.1">
        <property type="nucleotide sequence ID" value="XM_017725743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDB6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021056"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032055.2">
        <property type="protein sequence ID" value="ENSPNAP00000021056.1"/>
        <property type="gene ID" value="ENSPNAG00000028042.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444528"/>
    <dbReference type="CTD" id="560916"/>
    <dbReference type="GeneTree" id="ENSGT00940000154108"/>
    <dbReference type="OMA" id="DARDSCC"/>
    <dbReference type="OrthoDB" id="8777148at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008327">
        <property type="term" value="F:methyl-CpG binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040388">
        <property type="entry name" value="CXXC4/CXXC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13419:SF2">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13419">
        <property type="entry name" value="ZINC FINGER-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CXXC-type" evidence="13">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="289" mass="30349" checksum="EE6BE80A2C30F703" modified="2018-12-05" version="1">MSGSGGLMEGNQAREDEDARDSCCGEEDSSPVVERRNRSGIISAPLNKSLKHSRALSQYIATCSAAAAVANANANRLAQSSLVSTGVKAHSTAAQHRAQVGLTKLDRGALLSGLLDSPSGLHLAQAAELLRQASMLLPVSDSTGLNMSGDMEGVSASDSLGSVTDFPLLSNGGGVGGGFPFHPGLFIMTPAGVFLADGALSQVTGATEHQQTHSEITSAISANGKKKRKRCGLCPPCRRRINCEQCSSCRNRKTGHQICKFRKCEELKKKPAGGLEVMLPTGAPFRWFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDH3</accession>
    <name>A0A3B4DDH3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">tRNA (guanine(27)-N(2))-dimethyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">tRNA methyltransferase 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TRMT1L</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>guanosine(27) in tRNA(Tyr) + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(27) in tRNA(Tyr) + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:83895"/>
            <dbReference type="Rhea" id="RHEA-COMP:20240"/>
            <dbReference type="Rhea" id="RHEA-COMP:20241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:83896"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573965.2">
        <property type="nucleotide sequence ID" value="XM_017718476.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDH3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021535"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032896.2">
        <property type="protein sequence ID" value="ENSPNAP00000021535.2"/>
        <property type="gene ID" value="ENSPNAG00000004389.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439846"/>
    <dbReference type="GeneTree" id="ENSGT00530000063646"/>
    <dbReference type="OMA" id="CICHLSC"/>
    <dbReference type="OrthoDB" id="6349953at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000098">
        <property type="entry name" value="Trmt1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF1">
        <property type="entry name" value="TRMT1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 19" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 19" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 19" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="671"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="686"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021535.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="695" mass="77613" checksum="B8C0F2D6BA4542CE" modified="2024-11-27" version="2">MAELEEGDTVQLHQEDANIKVVDDLVKMQPASEESVQLSVHEATSTDIVKTEDHHEQTPATTSSERHVSIQSTLEGLEMLVDLNGAGRKSCPLCPEEKFKACYSHKLRRHLQNLHWKVYVEFEGQRMCICHLPCRQMKSSLGSDQSQARLVAHYHCVVCSVTIARKTDMISHLKRHINKGETEASYSGTSDVLCEEPVPGGQVYEIMKELGTNVQLMPNHNTPQKTDTYFNRKMKTNRQLVFCSLAVLAEERSPLECLDAFGATGIMGLQWAKHLRNAVKVTINDINEACVKMIKENCRLNHIRTEGGRAPRQVEGTGESDNPIATVEVSKMDANVIMHLRAFDYIHLDPFGTSVNYLDAAFRNVRNLGIVSVTSTDTGSLYSKSLNVTLRHYGCQIVRTEYYRELAARMVVASIARAAARCNKGIEVLLAVALEHFVLVVVRVLRGPTQADESAKKLRQLLHCQWCEERVFLKPGSMVEENLYRQLPCQCHGSMPGKTAVELGPIWSGSLFNTGFLRRMLVAAVQYSMEDIQPLVKTLICEADCTTLKSFSVTGHSVLANQVECGVVIKTLQKVEEADTADQSGKRKSEEPSNVVKKLKSDASLEHPAFYYSIHRHSIRGMNMPKLNKFLQYLTEAGFRVSRTHFDPTGVRTDATLEQFKSVLTKYSVPTYSTAPPSGNQGHGVSTDDKGRKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDH4</accession>
    <name>A0A3B4DDH4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RALB</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDH4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020989"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032015.2">
        <property type="protein sequence ID" value="ENSPNAP00000020989.2"/>
        <property type="gene ID" value="ENSPNAG00000027973.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155142"/>
    <dbReference type="OMA" id="WEVSYVE"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04139">
        <property type="entry name" value="RalA_RalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000203">
        <property type="entry name" value="Putative ras-related protein ral-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020989.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="207" mass="23519" checksum="9B66A6B965B0A32F" modified="2024-11-27" version="2">MAANKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITESESFAATSLFLREQILRVKEDENVPFLLVGNKSDLEDRRQVGVEEAKARANQWEVSYVETSAKTRANVDKVFFDLMRQIRARKMEDGKEKNGKKKRKSLANRIRERCCVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEW4</accession>
    <name>A0A3B4DEW4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017550770.2">
        <property type="nucleotide sequence ID" value="XM_017695281.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017550788.2">
        <property type="nucleotide sequence ID" value="XM_017695299.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017550799.2">
        <property type="nucleotide sequence ID" value="XM_017695310.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEW4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021459"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032771.2">
        <property type="protein sequence ID" value="ENSPNAP00000021459.2"/>
        <property type="gene ID" value="ENSPNAG00000006927.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426061"/>
    <dbReference type="GeneTree" id="ENSGT00940000170761"/>
    <dbReference type="OMA" id="HGEPRHS"/>
    <dbReference type="OrthoDB" id="5877502at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="629"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="629"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="687"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021459.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="823" mass="96198" checksum="2BD84611BC94DE99" modified="2024-11-27" version="2">MADLLLTVYDDDQSNVFFDCTICNRRLNGETQYKVHLTTLQHLKKEEAEAAKGLIPMPLPLPEWTDIKQYLKYLNLDEPIIGLGALVQEEDAVTDDGKVQLRYRCKMCAVVMDMCTMVCHIVSRKHRQKYLQLKRPDLVTWQKNSSQKQPGLVAKAKAALVEKQEGWGCPVALSRPNEGQRQKKDYQGHGREEDTHSYTHERSYSGRDGSGPFPLAEDGYKRSYLKDDALGKPSRYHEVDLYEISSKDNAAHGRSYPENDRCEMTNYQGGRQRYYEDDNCSEGAYLKGDISVRSFLEEDSSGRHQIDRTIPARSYSGKRQDECYPEINLRRGWNSNSDMQDMHHGGLPGSKFADTEMSGSSRYLEDTDYRQDRQIQDRMYTSCEGRGRSIDELQRPRRSFQYAEEERKHVPNVENTARAGSFEVQDYTQRHRDDLEDYTQEDRPAKRKRKSRFSDATPLEIALAQKRFFETMALKNPGRAVPTKSQDFHRWVDQRGDMQEERCLDDHGFQETTTYEHGHRVFQEARTFTNDSTSLQESRRFEDNSRNVKNERYFEDRRHIQDMKGYEKASYQESSKYKDLPRDFQESRQCEYDRTNFQQSRGFENSRDYQHGRCFVDGPASFQQAKEFKDKPKGFQRGGHFEDYPSGFQQARRFDDNPRVGHVDEPRSFQNIRQSRGTDKPIGYENYEGRRAGADGRYSEGDFRHSQASLDSQREFEWDPKRRGSAERSEDLKNYYPRYDNDDRRDTRVFSESLWKSRPQRKEEKVYGVRQAQRPRYQRDGASGAEPYDPFHPSSSPPQELAPPSSLEKLAVTLLELMARKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DF34</accession>
    <name>A0A3B4DF34_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucocorticoid induced 1a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">GLCCI1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DF34"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034041.2">
        <property type="protein sequence ID" value="ENSPNAP00000022115.1"/>
        <property type="gene ID" value="ENSPNAG00000029367.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183046"/>
    <dbReference type="OMA" id="WPRDSHE"/>
    <dbReference type="OrthoDB" id="10037581at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072015">
        <property type="term" value="P:podocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026642">
        <property type="entry name" value="Glcci1/FAM117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972">
        <property type="entry name" value="AGAP011572-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972:SF3">
        <property type="entry name" value="GLUCOCORTICOID-INDUCED TRANSCRIPT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15388">
        <property type="entry name" value="FAM117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="43497" checksum="32AECCC1DD76E2E8" modified="2018-12-05" version="1">MQLALLGDGGRSVVQAVQDSPRFSRRLARTEQRGSMPSPSFPSSAGGRAASRVKPGPGLCSGAPAESEAPQRRRVSSAGEGPPVQSSSMDLITGQYLCGQWPRDIHQTQRTCMTDKATQTPGFWSDDLEGLKIHKRSASWSSADHRGEIAKLRGQLRRSVLGIYHNGEQDSVSLFNQDQTEEPPASLTSLTVSKPAVCRVSSYTDSINHELESVFVSDTWGTQQERELQDGRCAPVPPHHQTQSMDTQLVSNGPHSPHLFPFSADLPQALDSFSSRPDFSEGHDYVNESSSPLPPLASSPKPNNSFIFKRVPPEGCEKIKVFEETLSKGFPVFSCPDRNKVSFVPTGSGPFCPVRQLDSSFFPSVCGSGSQSGQATGQFKQAGGRERPPPHCCGAYRTLHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFT3</accession>
    <name>A0A3B4DFT3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Neurotrophin-3</fullName>
            <shortName evidence="11">NT-3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NTF3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Seems to promote the survival of visceral and proprioceptive sensory neurons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the NGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017549380.1">
        <property type="nucleotide sequence ID" value="XM_017693891.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFT3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022360"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041179.2">
        <property type="protein sequence ID" value="ENSPNAP00000022360.1"/>
        <property type="gene ID" value="ENSPNAG00000029730.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425321"/>
    <dbReference type="CTD" id="4908"/>
    <dbReference type="GeneTree" id="ENSGT00390000007725"/>
    <dbReference type="OMA" id="FQPMIAM"/>
    <dbReference type="OrthoDB" id="6491780at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005163">
        <property type="term" value="F:nerve growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021675">
        <property type="term" value="P:nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038180">
        <property type="term" value="P:nerve growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000002">
        <property type="entry name" value="Brain-derived neurotrophic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020408">
        <property type="entry name" value="Nerve_growth_factor-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002072">
        <property type="entry name" value="Nerve_growth_factor-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045815">
        <property type="entry name" value="NTF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11589">
        <property type="entry name" value="NERVE GROWTH FACTOR NGF -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11589:SF4">
        <property type="entry name" value="NEUROTROPHIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00243">
        <property type="entry name" value="NGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19338">
        <property type="entry name" value="NTF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001789">
        <property type="entry name" value="NGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00268">
        <property type="entry name" value="NGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00140">
        <property type="entry name" value="NGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50270">
        <property type="entry name" value="NGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Nerve growth factor-related" evidence="14">
        <location>
            <begin position="148"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="199"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="209"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001789-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="260" mass="30088" checksum="2A82AFD7D076A54D" modified="2018-12-05" version="1">MVTFITILQVNLVMSILLYVMFLAYLYGISATTTSNRQRPTQDPINTLIVKLLQADIGRGRQRQDEGAQEAALPTTHPSEMQYPHRTTAKRAIADELLRQHKRYNSPRVLLSERPPLQPPPLYLTDDFVGGQDATNRTRQKRYAEHKSYRGEFSVCESESRWVTDRTTAVDTHGREVSVLTEIGMVWPSMKQYFYETSCKSSRPFKSGCQGIDDKHWNSQCKTSQTYVRALTKYNNMLSWRWIRINTACVCALSRKRRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DG98</accession>
    <name>A0A3B4DG98_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inactive tyrosine-protein kinase PEAK1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pseudopodium-enriched atypical kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Sugen kinase 269</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Tyrosine-protein kinase SgK269</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PEAK1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable catalytically inactive kinase. Scaffolding protein that regulates the cytoskeleton to control cell spreading and migration by modulating focal adhesion dynamics. Acts as a scaffold for mediating EGFR signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570469.1">
        <property type="nucleotide sequence ID" value="XM_017714980.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570470.1">
        <property type="nucleotide sequence ID" value="XM_017714981.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037398667.1">
        <property type="nucleotide sequence ID" value="XM_037542770.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000022126"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041008.2">
        <property type="protein sequence ID" value="ENSPNAP00000022126.1"/>
        <property type="gene ID" value="ENSPNAG00000029382.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437704"/>
    <dbReference type="CTD" id="79834"/>
    <dbReference type="GeneTree" id="ENSGT00940000157591"/>
    <dbReference type="OMA" id="MGWNRNR"/>
    <dbReference type="OrthoDB" id="9886644at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043535">
        <property type="term" value="P:regulation of blood vessel endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000437">
        <property type="entry name" value="Pseudopodium enriched atypical kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051511">
        <property type="entry name" value="MitoQC_Scaffold_Kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22972:SF5">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE PEAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22972">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1420"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="143"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="253"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="369"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="459"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="516"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="732"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1132"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1515"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="261"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="385"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="525"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="555"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="648"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="753"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="781"/>
            <end position="796"/>
        </location>
    </feature>
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        <location>
            <begin position="797"/>
            <end position="822"/>
        </location>
    </feature>
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        <location>
            <begin position="823"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="852"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="955"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="969"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1005"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1015"/>
            <end position="1027"/>
        </location>
    </feature>
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        <location>
            <begin position="1028"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1068"/>
            <end position="1078"/>
        </location>
    </feature>
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        <location>
            <begin position="1162"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1199"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1230"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1304"/>
            <end position="1314"/>
        </location>
    </feature>
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        <location>
            <begin position="1344"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1523"/>
            <end position="1544"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1861" mass="200698" checksum="CF4E2465AB4123F0" modified="2018-12-05" version="1">MSACSTFTEHVWKPGECKNCFKPKSLHRLAEGDSRKGRHEQAPVQHSQTPTGVRTNINQHSHSPVVGGANINTNIAISTQRSGGSRSGQFRPPVAKKPTIAVKPTMMLPCSGVGLDTEGNPQMPAEGAEPGKVSAFTVWSHNGLNRKRPAGPNNNEEDEEGGQIEGYASLCPPSSSSNNNNGLTDVLKEIAGLGPSPSSSRDDFLGRISCSYRRSLERGLPAAGCLAMGSSGRAAKHVSLSDSAEIISSEGGRFCYPEFSSEGEDDEEGAELSEGDDDDDEDEDDDGEHESWDESDEELLAMEIRMRGQPRFANFRAATLSPVPFAAGKKWNTVPLRNRSLQRICAVDYDDSYDEILNGYPSVDSNGAPGLLSYGSDPQGSGFLSNSESTTSPEFSSSLPDDSCTSSSGSSAPCGQRNGLHLASASKELTHQGDPVGRALTSPKATETHKAVLAIRLQEQDTSQREGGPLPQALPGQPITISFSPTEEQAKPYRVVSLEKTPICKPYTVVDVSASMATTEERASESAAKSKSPNPAFGPPPYPGASKNVPLCAGSPFSPKSPLSPKLPLTPKSVAVSPTSPFTPVSPSVQVSPSTAVAPSPSSKKPGNIRYQEVWTSSTSPRQKIPKVELLSGGTSGPSVPPRHSNHKSAPTSPVAGLSSSRTVPVKSPNLSEIKFNSYNNAGMPPFPIIIRDEPAYARSSKNAVKVPIVINPSAYDNLAVYKSFLGLSGEPPQHKPGAGSRVTSHTYEEIGSSESTQPSPTEQMPSARKPASATTEEERDTTRVVEAPHDDKAKSTTESNVVSTNFSVTFSTPRTNITPTVSPLSGSLTKSSSATANLSRSRNPSGEPPVGSSECQSSSTSGSGHREKASMVLSQIVASIQPPQSPPESPTAQGKTCSAEELYSLPPDATKGTLSRPKSLHCPADSGLSKSRKETPSKLLPKSQSASAAEAPTSPKPEPGAPFPPPRSTSSPYHATNLLQRHFSNWTRPTGSRPSDGEVSPGTEGRRSSDSNKPKRWISFKSFFRRRKDEDEQKEKAEREKEKGKLVGLDGTVITMLPPPPIQRQHWFSESKTDDPTQKPTIIFTYKPDSGAGGDGEGELRVEECNASTVTSLNQSSAPKSRASLLISKVMSQMPVEGPDKEMPAATSLPAKLELSPVCEAASPSTQPAAPPLSHSEGSASLGEPEEDEGTHTHSHSHSPASSSCSATYTNLGQSRASMIPLKHPRHPKASDDTLHSEPEGSDPGSKATPPPLPKKAVPRAHTEPSLSRESAAPRPRGEAKPGGTSLSVANPLYDLDSTWETASQSSSLSSDARQLDESGDSLERPTGGRSLSCLTNSGSAQGRERKASERRGCRSTESLTARGRSSGRGRDGGSKPQRQALYKGIDSWEEVVGRIRSLHTETLCKLASRCEDRFMAGQKDHLRFGTDSWSHFRLTTGKPCCEAGDAVYYTAAYAKDPLVNYAVKICRYKVKETQQQFFHSLAVRQSLPVHFNIQQDCGHFLADVPARLLPWEEAESDEEKEEKGEKGEKKSEGGEEEWKVGRSEEEALVDTKLRSRVVVITREVPFQTVADFVRDGVGRHARNPELYERQVCLLLLQLCAGLEHMKPYHVTHCDLRLENLLLVHCQPGNPWNLDLLEPNNNNSATASASSSACPARLIISNFSQAKQKSSLAEEPSVVLKDRSRLAPELLSATQYRKCDEFQTGILVYEMLHRPNPFEEAPGLKEREYARSDLPQLPTRSLYSQGLHRLAYLLLDANPSERISMADARACLQCLLWGPREDLFHTLGTGATPAQRQTTLQNWLDLKRTLMMIKFAERSLDSGCGVSLEDWLCCQYLAFASTDSVDRVIRILQEPQGQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGU5</accession>
    <name>A0A3B4DGU5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGU5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034064.2">
        <property type="protein sequence ID" value="ENSPNAP00000022129.2"/>
        <property type="gene ID" value="ENSPNAG00000004610.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155555"/>
    <dbReference type="OMA" id="HYQERNH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021960">
        <property type="term" value="P:anterior commissure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021961">
        <property type="term" value="P:posterior commissure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF7">
        <property type="entry name" value="KINESIN LIGHT CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
        <property type="entry name" value="KINESIN_LIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="299"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="341"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="199"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="516"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="598"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="133"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022129.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="657" mass="74098" checksum="A8371E302C0F4E91" modified="2025-10-08" version="3">MISCKYILCPALSALCPINTGCTAYIYIKIKTVSGLYSMMREDMSSVVCLKEVEGQGDPAEKLSQDELLCRTREVMQGLEALRAEHQAILEGLLGTLRCLKQSQEGRAVEEKTAMIQRSMEMLELGLSEAQVMMALSGHLSAVEAEKQKLRAQVRRLCQENQWLRDELAGTQQRLQKSEQSVAQLEEEKKHLEFMNQLKKYDQDLSPTDDKDSDSSRETLDDLFPDEQDEPGPGIQPPHSSAVAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKLAETLYKEILTRAHEREFGSGKQKDGSPFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDTAHKQRVAEVLGDPEAREKQRSRESLTSDTVKYESGPDGGEEVSMSVEWNGDGSGSLKRSGSFSKLRASIRRSSEKLVRKLKGGSSRENEPKNPGRSNKVLKIERWQFYGVWEKIMKKVISERLWNEHEYEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHN0</accession>
    <name>A0A3B4DHN0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Podocalyxin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Podocalyxin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PODXL</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the podocalyxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567679.1">
        <property type="nucleotide sequence ID" value="XM_017712190.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHN0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022429"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041219.2">
        <property type="protein sequence ID" value="ENSPNAP00000022429.1"/>
        <property type="gene ID" value="ENSPNAG00000029797.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435999"/>
    <dbReference type="CTD" id="5420"/>
    <dbReference type="GeneTree" id="ENSGT00730000111314"/>
    <dbReference type="OrthoDB" id="9948358at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022408">
        <property type="term" value="P:negative regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033634">
        <property type="term" value="P:positive regulation of cell-cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032534">
        <property type="term" value="P:regulation of microvillus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013836">
        <property type="entry name" value="CD34/Podocalyxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017403">
        <property type="entry name" value="PODXL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12067">
        <property type="entry name" value="PODOCALYXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12067:SF5">
        <property type="entry name" value="PODOCALYXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06365">
        <property type="entry name" value="CD34_antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 21" id="KW-0732">Signal</keyword>
    <keyword evidence="11 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017245654" description="Podocalyxin" evidence="21">
        <location>
            <begin position="24"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="27"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="28"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="95"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00007029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="447" mass="47970" checksum="24EACD2D8E19F18B" modified="2018-12-05" version="1">MWTKTRMLWTIFVLGFLLQYTNAESIVPTNSSAPSTTRSSSSVSMTTTVQPSTSAPLNTSTLTSPPSSAASTTLKSIPSSASTTTAVEATTPTSRAQPTSKILHSVSQGSTPSSTPQATITITVNSGASPQSTTSVKATTSNSTTAQGSTQTKAPNTPSSSTGNHPTTPNIRITSVSTSSDLPGPIAQKDSFTQSTTAKISTIIPSILSTLNSKHTRPSVTDSPIDKSIQITATTNQNTEAVTKQVTHQNSIPTTALNRSPSTGTIKPFNMEITLEKIQNATNNDVFVRTCRKLLKNYNAASGCTLEVNKRGDDDFAITSASLTVDPNEVNSIYSKEKMSEHQPTPVPSTLIAILSSCGALLLFLVCFALYCVCHHRSYRKNQQHLTEELQTVENGYHDNPTLEVMEVQPEMQEKKLTLNGEFNDSWIVPIDNLAKEDIPDEEDTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJ90</accession>
    <name>A0A3B4DJ90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Solute carrier family 22 member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Organic cation transporter 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(R)-adrenaline(out) = (R)-adrenaline(in)</text>
            <dbReference type="Rhea" id="RHEA:73875"/>
            <dbReference type="ChEBI" id="CHEBI:71406"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(R)-noradrenaline(out) = (R)-noradrenaline(in)</text>
            <dbReference type="Rhea" id="RHEA:73871"/>
            <dbReference type="ChEBI" id="CHEBI:72587"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(R)-salsolinol(in) = (R)-salsolinol(out)</text>
            <dbReference type="Rhea" id="RHEA:74791"/>
            <dbReference type="ChEBI" id="CHEBI:194082"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-histidyl-L-proline diketopiperazine(in) = L-histidyl-L-proline diketopiperazine(out)</text>
            <dbReference type="Rhea" id="RHEA:74787"/>
            <dbReference type="ChEBI" id="CHEBI:90039"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>agmatine(out) = agmatine(in)</text>
            <dbReference type="Rhea" id="RHEA:72131"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>dopamine(out) = dopamine(in)</text>
            <dbReference type="Rhea" id="RHEA:73863"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>guanidine(out) = guanidine(in)</text>
            <dbReference type="Rhea" id="RHEA:73883"/>
            <dbReference type="ChEBI" id="CHEBI:30087"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>histamine(out) = histamine(in)</text>
            <dbReference type="Rhea" id="RHEA:73879"/>
            <dbReference type="ChEBI" id="CHEBI:58432"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>serotonin(out) = serotonin(in)</text>
            <dbReference type="Rhea" id="RHEA:73867"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>spermidine(in) = spermidine(out)</text>
            <dbReference type="Rhea" id="RHEA:35039"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>tyramine(in) = tyramine(out)</text>
            <dbReference type="Rhea" id="RHEA:74783"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555460.1">
        <property type="nucleotide sequence ID" value="XM_017699971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017555461.1">
        <property type="nucleotide sequence ID" value="XM_017699972.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJ90"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022999"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013745.2">
        <property type="protein sequence ID" value="ENSPNAP00000022999.1"/>
        <property type="gene ID" value="ENSPNAG00000000292.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428726"/>
    <dbReference type="CTD" id="6581"/>
    <dbReference type="GeneTree" id="ENSGT00940000155089"/>
    <dbReference type="OMA" id="LILTYAW"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008504">
        <property type="term" value="F:monoamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005326">
        <property type="term" value="F:neurotransmitter transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015651">
        <property type="term" value="F:quaternary ammonium group transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015872">
        <property type="term" value="P:dopamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051608">
        <property type="term" value="P:histamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015874">
        <property type="term" value="P:norepinephrine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006837">
        <property type="term" value="P:serotonin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000165">
        <property type="entry name" value="Solute carrier family 22 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="494"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="29">
        <location>
            <begin position="98"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00036754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00036845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00036998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00037001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00072456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00079877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="544" mass="60665" checksum="1411FF258A49DE88" modified="2018-12-05" version="1">MMPNFDELLQHAGDFGPFQKRIAFLGTLPVSLFAFVLVGVVFLGNTPDHWCKIAGAAHVQEDCGVSVEEVRNLTVPHAGPHGSHNRCAKFDMDWNFSAVSCSDLSHWSIPTNTTPVSSCDEGWMFDLTWTTVVTEFKLVCEQSWLADLNQASLAAGFFIGAFVSGYVADRFGRKLCFIGSILGLGVSGMCIIISPYYPLFLVFRCLQGFFAKGAWTTTYVLVIEFFGSNNRKFVSVMSRTLYSTGMVFIPGLAYYIHSWKNLQLTMTLPIFIFLVYYWVMPESPRWLLSQGKTREAMSIVENVAKCNKKSMPTDLPEMDVLVGQQEIVPPSFKDLFRTPKMRKHTIILIYAWFTSTVVFQGLLLRLGITGDNVFLDFFISAVVELPTGLIFYLLVDRVGRRPLLTAANLVAGVACLIVPFIMQNFSWLRRSIAIIGRLAVAIGFETVNFTNSELYPTPLRNLGVSFCSSASDIGAVAAPFVLYRLASVWQELPLLLYGVMSIVYSGLVMMLPEMKGVDLPETIDDVENRGKKDEQKVGKSNNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJI3</accession>
    <name>A0A3B4DJI3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FIG4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJI3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023089"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013549.2">
        <property type="protein sequence ID" value="ENSPNAP00000023089.2"/>
        <property type="gene ID" value="ENSPNAG00000007629.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074943"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="6">
        <location>
            <begin position="155"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="708"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="764"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023089.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="910" mass="104856" checksum="EABD234F0A0AF18F" modified="2024-11-27" version="2">MTKRQFFWYLTFCTVVIGSVTNTRYFLVGSNNAQTKHRVLKIDRTEPRDLVVIDDKHVYSQQELRELLGRLDLGNRTKIGQKGSSGLSRAVSAFGIVGFVRFLEGYYIILITKRRKVADIGGHSIYKIEDTNMIYIPNDSVRVTHPDEARYLRMFQNVDLSSNFYFSYSYDLSHSLQYNLTLLRIPCEPAEVEGLHSSCSKQDSFDIFEDEGLPTQVGTGVMVHGLKNEPYAKYVWNSRLLERIKDTVHPDWLMYIIHGFCGQSKLLIYGRPVYVTLIARRSSRFAGTRFLKRGSNCEGDVANEVETEQIIHDASVMSFTAGSYSSYVQVRGSVPLYWSQDISTMMPKPPIRLDQADPYAHVAALHFDQMLQRFGSPIIILNLVKKREKRKHEKILSEELYRAIINLNQFLSPEHCIDYIAWDMARYTKSKLCNVLDRLGMIAENVVKRTGFYINRSDFHCHTLRPDERWGDLGGLVVAHGRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYALGMIDKPKLQFDTDCVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDAINLFLQVYQPSESKPHLWELPTDFYLHQSNTMALPQERRSYTYWWSDGVLSYLPLPYDEVPCEKNKKKVLVKRVNRYEESTDMYTEFFKPYELTSFDDTFCIAMTNSAREFMPKSVGLDPSPFTVRKPEETGKSVLGNKSNKEEAVLQRKTAASAPPPPSEEQISSSSEDDSEEDREDDATASQRSTPIKIGESGDNVRTQEHQQQPARETYGLNLSALPRDEDFFIYHRSVCLESDSFINFSVINCCLCICGYYRVPVSSFDGDSIYEMLPPRVDLESRNLFENHIHAGQGLIRPLCREDLLLYREYLKNRYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPH4</accession>
    <name>A0A3B4DPH4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">39S ribosomal protein L39, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRPL39</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the mitochondrion-specific ribosomal protein mL39 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557386.1">
        <property type="nucleotide sequence ID" value="XM_017701897.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPH4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025365"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007780.2">
        <property type="protein sequence ID" value="ENSPNAP00000025365.1"/>
        <property type="gene ID" value="ENSPNAG00000010414.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429847"/>
    <dbReference type="CTD" id="54148"/>
    <dbReference type="GeneTree" id="ENSGT00940000156271"/>
    <dbReference type="OMA" id="YNCAQHL"/>
    <dbReference type="OrthoDB" id="5870821at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000006">
        <property type="entry name" value="39S ribosomal protein L39, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050062">
        <property type="entry name" value="Pro-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME PROTEIN L39/PROLYL-TRNA LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00071662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="336" mass="37165" checksum="A6E459669BA222F8" modified="2018-12-05" version="1">MACGRVGQMVQRRLASGATAERISAAEVRSWQSALFSKERDRQQSLYPRIEKIEVTMQGPGLQGTLLVMNKGLSTPHSCARHLTEWHLSSAALALVDGEPWHLHRPLTRSCSLTLLTFKDNNPQLANQAYWRSCAALLGQVLDNAFKEEYSVELLQIPEVPVTAGAFCCDLVLDPRLDSWIPSEESLRSLTREAHQLIHKDLPWEPLEVTPPVALEIFSHSRCKQKEVEEEASQSPSGTVSLYRCGDHVLLAGGPLVARTGLCSQYEVTALHTVGEGQWGLQRRAQGLSLPFNLTAHHSVWRKLRKRAEKLVEIQSSAPSESVTTPTPPEATPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPZ1</accession>
    <name>A0A3B4DPZ1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbonic anhydrase Xa</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Does not have a catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPZ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025525"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007401.2">
        <property type="protein sequence ID" value="ENSPNAP00000025525.2"/>
        <property type="gene ID" value="ENSPNAG00000010679.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155223"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03121">
        <property type="entry name" value="alpha_CARP_X_XI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000002">
        <property type="entry name" value="Carbonic anhydrase-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041878">
        <property type="entry name" value="Alpha_CARP_X/XI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF208">
        <property type="entry name" value="CARBONIC ANHYDRASE XA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="3">
        <location>
            <begin position="35"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025525.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="337" mass="38415" checksum="D4FBC3DF09A21649" modified="2024-11-27" version="2">FSFPAVVSGASFEDLDMFKMFEFLSAQPNPKIHEGWWAYKEVVQGSFVPVPSFWGLVNSAWNLCSVGKRQSPVNIETSHMIFDPFLTPLRLNTGGRKVGGTMYNTGRHVSLRLDKEHLVNISGGPMTYSHRLEEIRLHFGSEDGQGSEHLLNGQAFSGEVQLIHYNHELYTNYTEAAKSPNGLVIVSIFMKISETSNSFLNRMLNRDTITRITYKNDAYLLSGLNIEEVYPETSSFITYEGSMTIPPCFETATWILMNKPIYVTKMQMHSMRLLSQNQPSQIFLSMSDNVRPVQPLNNRCIRTNINFSMQGKDCPNNRAQKLQYRGNVWLLLVKLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DT65</accession>
    <name>A0A3B4DT65_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G protein-coupled receptor 137Bb</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 137BB"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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    <name>A0A3B4DTI4_PYGNA</name>
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        <recommendedName>
            <fullName evidence="8">Microtubule-associated protein 6a</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the STOP family.</text>
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    <dbReference type="Ensembl" id="ENSPNAT00000001919.2">
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        <property type="gene ID" value="ENSPNAG00000002634.2"/>
    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DV74</accession>
    <name>A0A3B4DV74_PYGNA</name>
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        <recommendedName>
            <fullName evidence="8">PHTF1/2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PHTF1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017567967.1">
        <property type="nucleotide sequence ID" value="XM_017712478.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DV74"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026976"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003761.2">
        <property type="protein sequence ID" value="ENSPNAP00000026976.1"/>
        <property type="gene ID" value="ENSPNAG00000012360.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436152"/>
    <dbReference type="CTD" id="10745"/>
    <dbReference type="GeneTree" id="ENSGT00390000011648"/>
    <dbReference type="OrthoDB" id="10066656at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039775">
        <property type="entry name" value="PHTF1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021980">
        <property type="entry name" value="PHTF1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12680:SF8">
        <property type="entry name" value="PROTEIN PHTF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12680">
        <property type="entry name" value="PUTATIVE HOMEODOMAIN TRANSCRIPTION FACTOR PHTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12129">
        <property type="entry name" value="PHTF1-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="461"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="PHTF1/2 N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="145"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="696" mass="77863" checksum="20CF05AE2FCCE931" modified="2018-12-05" version="1">MSRIAWYQEKIGAYDQQIWEKSLEQAELKGIRNKPKKTGHIKPDLIDVDLVRGSTFSKAKPVSPWTALTRKGLVRVLLYPFFFKWWIQVTSKCIATGILLLYFLQVAAVVLYIDVPSAAASEVVGPMCLMLLLGTVHCQIVSTESCRSSDGSPVTSPVTSQTSSRATSPVRRKRQRKGRAVNKANDGDGGTALKWHLKENQRFYESEKSRNSQRQSVDRLSEDLSSEEEEGEDTEQARGKLIPPVQAKPAPGLRKRLCIANKGSSAHIKQHQVTANREAGNVTTVTTATPLEVKNLRPSEDSRPASDSDDILWEELLQGSDSASSASTDSGEEELHCHNHALSPPTILTSDEDEAFSQHQLSWLQACHPSKDRVSAIFWEKGECKKADMSVLEISGIILTRVKVVEQGMGYLVLGGLVTATLALLPFCFRLVQQLDVSRLSSITLHELAIMAAGKLSTGSYAFFFITTVERVFLTGLFFFMMCVAERTYKQRLLFAKLFSHITSARKAKKSEIPHFRLKKVQNIKMWLSLRSFLKRRGPQRSVDVIVSSIFLLALSIAFTICAQLLHSHHTFLDWESNWELLVWGAALLVFLLRLATLGAETNHKYSNASVLLTEQINLYLKMEKKPDKKDELSIVNNVLKLATKLLKELDTPFRLLGLTVNPLIYNITRVVILSAVSAVVSDLLGFNIRLWKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DX47</accession>
    <name>A0A3B4DX47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">HIKESHI</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DX47"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001080.2">
        <property type="protein sequence ID" value="ENSPNAP00000028005.2"/>
        <property type="gene ID" value="ENSPNAG00000013563.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000199379"/>
    <dbReference type="OMA" id="NNDAWIG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043949471" description="C-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="58"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028005.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="172" mass="19574" checksum="52D07117DCDDC73A" modified="2024-11-27" version="2">TMNTLVLLCLFCISFSHGAPAGEQHFTFIPTHRMFIKYIRMFSVFSTFVQCPTGWFRHGSRCFLLVKSRMSWLNAENHCVSQQGSLASVQSPAEYQFLQNLADLGGQTSAWIVFSLQNTWMWIDRAGFYYSNWQSLSSVSSNPCAYLQTQAGWANTNCATTLTFFCVRIPQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXS5</accession>
    <name>A0A3B4DXS5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin T, slow skeletal muscle</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Slow skeletal muscle troponin T</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TNNT1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with TPM3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXS5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001610.2">
        <property type="protein sequence ID" value="ENSPNAP00000027799.2"/>
        <property type="gene ID" value="ENSPNAG00000014014.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160609"/>
    <dbReference type="OMA" id="EWIYELE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031014">
        <property type="term" value="F:troponin T binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031444">
        <property type="term" value="P:slow-twitch skeletal muscle fiber contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000001">
        <property type="entry name" value="Troponin T, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF6">
        <property type="entry name" value="TROPONIN T, SLOW SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="113"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027799.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="269" mass="32291" checksum="CCD8067944AE005D" modified="2024-11-27" version="2">MFIFQIFNDLIVQRIQWYSGEKCSQDKTYRVLNICWCGRGHFLKTLFIPPMHSQLGAPKIPEGERVDFDDIHRKRMEKDLLELQTLIEVHFDQRKREEEELIALKERIENRRAERAEQQRVRAEKERDRQTRIAEERQRKEDEEAKKRADDEAKKKKVLSNMGAHFGGFLAKAEQRRGGKRQTGREIKRKTLAERRKPLAIDNLREDGLRERAREMWEWIYQLESEKFDLMEKMRRQKYEIKVLLNRISHAQKFKKGTGGKGKVGGRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZU2</accession>
    <name>A0A3B4DZU2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ISL LIM homeobox 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZU2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028586"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014888.2">
        <property type="protein sequence ID" value="ENSPNAP00000028586.2"/>
        <property type="gene ID" value="ENSPNAG00000014364.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153731"/>
    <dbReference type="OMA" id="AMHPNEV"/>
    <dbReference type="OrthoDB" id="125004at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035476">
        <property type="term" value="P:angioblast cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048665">
        <property type="term" value="P:neuron fate specification"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0048845">
        <property type="term" value="P:venous blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09366">
        <property type="entry name" value="LIM1_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09374">
        <property type="entry name" value="LIM2_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000034">
        <property type="entry name" value="Insulin gene enhancer protein ISL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000041">
        <property type="entry name" value="insulin gene enhancer protein ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000068">
        <property type="entry name" value="Insulin gene enhancer protein ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047169">
        <property type="entry name" value="ISL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047244">
        <property type="entry name" value="ISL1/2-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204:SF2">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 11" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="25"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="88"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="190"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="192"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="331"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
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    <evidence type="ECO:0000256" key="11">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028586.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40613" checksum="B876DE8313B6806C" modified="2024-11-27" version="2">MVDILLHSSFLGDMGDHSKKKSGIAMCVGCGSQIHDQYILRVSPDLEWHAACLKCAECSQYLDETCTCFVRDGKTYCKRDYIRLFGIKCAKCNSGFCSSELVMRARENVYHMECFRCSVCSRHLLPGDEFSLREEELLCRADHGLLLERGAAGSPLSPANIHSARALHMPAEPVPVRQPPHRNHVHKQSEKTTRVRTVLNEKQLHTLRTCYNANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKKSILMKQLQQQQHSDKTVSYRPDGLTGTPLVAGSPIRHDNTVQGNPVEVQTYQPPWKALSEFALQSDLEQPAFQQLVSFSESGSLGNSSASDVTSLSSQLPDTPTSMVASPVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E096</accession>
    <name>A0A3B4E096_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-actin monooxygenase</fullName>
            <ecNumber evidence="5">1.14.13.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Mical family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.225" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4E096"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015956.2">
        <property type="protein sequence ID" value="ENSPNAP00000029133.1"/>
        <property type="gene ID" value="ENSPNAG00000003359.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158780"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120501">
        <property type="term" value="F:F-actin monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF39">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14 18" id="KW-0440">LIM domain</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="20">
        <location>
            <begin position="515"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="21">
        <location>
            <begin position="981"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="623"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="725"/>
            <end position="771"/>
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<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2W4</accession>
    <name>A0A3B4E2W4_PYGNA</name>
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        <recommendedName>
            <fullName evidence="15">[histone H3]-lysine(4) N-methyltransferase</fullName>
            <ecNumber evidence="15">2.1.1.364</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="2052"/>
            <end position="2070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="2084"/>
            <end position="2095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030095.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2998" mass="331643" checksum="13C1CDD78736A284" modified="2024-11-27" version="2">MAAAGGGLPASVVTVGANPSAPARCRFPGRPWASRSQLKMERRLRVGRLGAEDRATRSRGPRPINVGNTLQEDPCLLRLLELAEKLGRQKEAGFCTSGSDEEGDFTGFQADGRWSLCSGHGSILKSSSAPSKENPALAPDSSSDDKKATTGKKLLRATAEAEQPEKKCVKTLRKDHKIERPKFVVKLASKNVTKKNASGRQQVKKASLRGVREKTAQKGIEFSHKQENGGVDEVLDKPGNEVAAAEDERKGRRQDEAIILKNRTRQKKLVWTLTLVKGKRRTSKKSLADNLDQSPSGGFVKTADLDKSEDGKTKDVLTTDDSTSPKTTGKRRKNPKAMRASLEVRTEAGQEDAVPPDDALLRENSSHSPKKSPTVVKRRKSLFGYRRTPEQELMRKMRESQLQGEGKIPRKRRRLVCYAYEVVDSPVREDQQQELPGQAEDAGVASQQGLAEGGVMSARPSRVIKLPKRFMDDEGMSGLPVKKPAQTEILPNESGIELEQIDVQQTQEFKRRQKRAKLHNISDGDGNFVGKAKSQGQKPRGVTEMSDGPRKKVGRVAYDSTHLKIYERLKKLTASLAQRRQKRMASYKDDGVKLEGELQGTEESSCFRDIGSQRNSDLKIEEVNSPGVVRKLAVHVDDTAVQSAVSTVEGGEDAAKAVVEGTSLEGTDVQRQDAGDSQVSVEQVSPIQRLNLSGANKRMLHLLKRAKVQLIKIDQQKQLKSCQLLSSAVRGEDGAIRGIPRRRKGAGRGNAQSAPQGHPIGGPRIKHVCRAAAVALGQPRAMVPDDIPRLSALPLHEREGIAPSPNTEDLGSCSDPESTGSAEQKPVSSRRSFGLRQKRCFRCKGCCREEDCGRCVFCLDKPKYGGPNKKRQSCIYKKCARIEENKMKRLKVQMKRRQISAAAYPCSSGEEEGEQSGAKEEESSQVDPASTLSPTRRQPRRRVTPRCYSNLLESDSTDTEPANGDTGKEESSSAPTDQANDTAAPPNARYEVGKPRKPGLPRGLWGRRRIDKSSAEHTPPSVLAALANGFTQREPQAQESTHKIRVDFKEDCNIQNVWAMGGLSILTSIPITPEYVCLLCASKGHHEMIFCQMCCEPFHRFCLPVDDRPQKDNKENWCCRRCKYCHVCGRKSKHGKPVLQCKRCLYCYHPSCLGPTYPKPAKCNTPWVCMMCIRCKSCGVTPGKSWDMAWNHELNLCPQCSNLHSQGNFCTVCLKCYQEHEFDSKMMQCARCAHWVHPKCEGLTDDLYELLCRLRGKSLVFSCAACSKSHPSGWQEVVQDVLRDGLEKVMSGLLNSLTTCHLRTCSQCEACPDAESIKDRKMVCDIRAVERKLEEGLYTTLKMFHEDLARLLMQKLQEEEHLSEEQRPTVQAKIFYLRLLEQTFSWFNSQDPVTWKPLLKEFPSGMLPEAIIPPSDEHSYAQWLEEQDRAITRTKETQENVHLKAHDPLNLNRRDIDERQCSLCQQHGDAKPNEAGRLLYLGQNEWAHVNCCIWSAEVQEVKGALLHVHSAVTRGRFMRCERCSQVGATVGCCLSTCQSNYHFMCARASHCVFQSDKKVYCHKHRELINNKMMNGFEVLRRVYVDFEGISLRRKFLTGLEPESVNVMIGSLQVNRLGVLSDLSEVSGKLYPVGYQCTRWYWSTVDPRKRCRYTCKVTDVQLSSPMRTHNSALNQEENCTIAHSPKPNEDMDSTNVETGPVDLLPGTPSPNSKLDSGAESKTPKHPQNRRPVGGTFRPLPSPGAAVSTSHHILTISDLDETRRSKRLSVRSRNASPPQKSSTVPMKLRSGGTLNPRSLTFSSPISPLGATENLMSSPSNRRRGRPPSSPSNATSAYSPRQGTIGSSPSTVYLSPCHSPRIQQHFRSTPQESAEVPQDFSASLEPEDAAGMPEDSISTIAVIGDGNSVPLPSDQELLSTHFDADTDVAVASVLNAKLEFDEALLNENVALHCGLYGSGAEVQEIRESQGAHTEGNSLLGRISDEDTSIYSASEKDLPGLSDPGGHIEVDSVDGDSNHYLNFSRTVVVCDAVKDSAQAGLTVLATSQSISQLDGADNDSESDASEASGDDDTQEVGNSYHSQETEPSKSVTPNSGRELVCTSTGMSFVPESIQIDVEPEVFQKTAVDQLSDKQELQDSVPLQEGWDVLDSATEVFTAQESISTQDLTVQEMQSVSEAVIENVLPTSPVFDPSIDLVAGQEDVLGDSKPVEDLNEVLLDPEMGHFVSAKDGSIVHMFDSSSVNSVNKDAKSPEKIVFPEPTVKVISAPSLSSVRKVTGPGPLPHKTYVIPQPVPQHRIVKMTVPAGTSLPLSVPVNMVSTSPAASVFQKNVVVASSQVAVNGLDSPKESPRSRTLAIRIPASRVSSGTGALPSPQVLLVNRSGQVLIKDPQTNTYQLPSTNSPSFSHIRQIAKIIHSSNLVQRSVPRVLVTPVSQASPSQAPTTHVSYSNGAVPSTKVFIRKLPQKFSEVQMNSGVRLNNTAVPVLPVSEVNQGDDAQAIIERAMASHREVANPGVLSPSQFQVHPYLSKLHSPDMVEHSSKLQHRTQPAILSRSKSQVRVKRVSSASERSGIKKSKTDFIEPTVSSSQDDLNRFNKVRIKAPTIKDMLDFDHPEAENLSEPKTAEAKDFENKRVAPIECESSEPQDRERAIHDKTHEWVSSTNSDLADWTPCADWSSDEDSPLPFKQEQNECAGQNQPHLVFKITSDDGFSVEADSIEVAWKAVVDGVQEARMGYRLEQLPLGRMSGARVLGVIHDAVLFLLEQLQGAPQCQNHRFRFHQHEKPEKELPVNPSGCARAEVYVRKSTFDMFNFLASQHRQLPESRPCDEEEDDIKLKSSRRATSTELPMAMRFRHLEKTSKEAVGVYRSAIHGRGLFCKRNIEAGEMVIEYAGNVIRSVLTDKREKYYDSKGIGCYMFRIDDFDVVDATMHGNAARFINHSCEPNCYSRVINVEGRKHIVIFALRKMYRGEELTYDYKFPIEDASNKLHCNCGARRCRRFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3Y6</accession>
    <name>A0A3B4E3Y6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576233.1">
        <property type="nucleotide sequence ID" value="XM_017720744.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3Y6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030460"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018613.2">
        <property type="protein sequence ID" value="ENSPNAP00000030460.1"/>
        <property type="gene ID" value="ENSPNAG00000017189.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441294"/>
    <dbReference type="GeneTree" id="ENSGT01150000286918"/>
    <dbReference type="OMA" id="QTSERIM"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000755">
        <property type="entry name" value="zinc finger protein 174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017306896" description="C2H2-type domain-containing protein" evidence="13">
        <location>
            <begin position="23"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="61"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="139"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="342" mass="38248" checksum="AC60B53CD697B080" modified="2018-12-05" version="1">MADSYFHVQLTAILDVLLKTAAVDICALADSVFTSLQREIERCTCENEELRRQLRSFTAEQNGLSDREAAPLKTEERSSRAAEDGGRKARDRRPQACNVETDAGTVNSGQQLQKQSAASAGVKEEVTEVYVTQSVESRVHDVSSDGVPVADRNAQLSDLSPDLKTESEKSESVFHSSQPDWNDCSITDYEEEREGQQENQNTQIRPYTNSNGEGEERETEKEQLRRETCPPQEPDLHCTFIQIDGTVTTSVPERQTGSTFACDICGKVVLSEQRLRVHVKTHATLRPYTCSQCGKSFTRKATLNFHQNIHRGVKPYACSVCPKSFADPSALRRHKSIHKVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E5S0</accession>
    <name>A0A3B4E5S0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_017573156.1">
        <property type="nucleotide sequence ID" value="XM_017717667.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5S0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031962"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021433.2">
        <property type="protein sequence ID" value="ENSPNAP00000031962.1"/>
        <property type="gene ID" value="ENSPNAG00000019613.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439325"/>
    <dbReference type="CTD" id="100126011"/>
    <dbReference type="GeneTree" id="ENSGT00940000164858"/>
    <dbReference type="OMA" id="IPEMMDQ"/>
    <dbReference type="OrthoDB" id="275301at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06653">
        <property type="entry name" value="STKc_MEKK3_like_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000071">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000026">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="360"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="224"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="624" mass="70574" checksum="30EC94E2E8D6210F" modified="2018-12-05" version="1">MMTVNMDDGLQNRPGPHRNTQDDEEALNSIMKDLAALRCCYQLNSNNSHKPKSRTQTYRQDLRVKLEHEREKRIIPFQRPLKFKELLQKVMEAFGQQMDLYYTDKETLVALKCQEDLDRAVLSLSSSSGTNGLLRVLLKTPTNNHFLQVNSRDKQSEMKSSRSLGDLKGSLLKGPERVRKHSTGSLHTGRSSPPPGSVPEEQQQIARQGSYTSIHSEGEFIPEMLDPNIMDPFGSADDSLSSSCKSLDQALDSPPFSQNNRDNNYLNLNFDYKGRHGKHGTFPRQFHISNRSKDYGDGRRTFPRSFMPQENLFQLVPSSRTRSYNGESTLQYSADMHSISWTEKSSQRSAAKSPRAPVNWRQGKLLGRGAFGEVYLCYDADTGRELAVKQVPFDPDCQETSKEVNALECEIQLLKNLRHERIVQYYGCLRDPAQRKLSIFVEFMPGGSIKDQLKAYGALTEKVTRRYTRQILQGVFYLHSNMIVHRDIKGANILRDSSGNVKLGDFGASKRIQTICMSGTGIKSVTGTPYWMSPEVINGEGYGRKADVWSVACTVVEMLTQKPPWAEYEAMAAIFKIATQPTKPNLPEGVSDACRDFVRQVFVEEKWRPTAEVLLSHPFVQGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7Q9</accession>
    <name>A0A3B4E7Q9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Discs, large (Drosophila) homolog-associated protein 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037387165.1">
        <property type="nucleotide sequence ID" value="XM_037531268.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037387166.1">
        <property type="nucleotide sequence ID" value="XM_037531269.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037387167.1">
        <property type="nucleotide sequence ID" value="XM_037531270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7Q9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031810"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021129.2">
        <property type="protein sequence ID" value="ENSPNAP00000031810.1"/>
        <property type="gene ID" value="ENSPNAG00000019411.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435716"/>
    <dbReference type="CTD" id="569898"/>
    <dbReference type="GeneTree" id="ENSGT00940000156220"/>
    <dbReference type="OrthoDB" id="10036956at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099572">
        <property type="term" value="C:postsynaptic specialization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005026">
        <property type="entry name" value="SAPAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353:SF7">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN DAP SAP90/PSD-95-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03359">
        <property type="entry name" value="GKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="923"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="928"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="952"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="981"/>
            <end position="990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1004" mass="110387" checksum="13AC932C6600DB2B" modified="2018-12-05" version="1">MMKGLSGSRSHHHVVACEPSYDPLGHHGDRKPYLIGQIDAPPPPEHPYYSGRSSYQPDCVAVPYGTFPRRHYSSSHELKDECALVPYTGGGASKGQQRVPVNLLEQLDRQLPVSRDGYHTLQYKRAAAAAASAALEQRNDSPGRIRHLVHSVQKLFTKSHSLEGPHHSKGSVNGGGGGGGKASPEGEGGPAPTPRQRKRSKSRERCKSAEPKQRAPPSGYWSSDEPERDICPLRHAPAPPGVMTMGRHPDKSQSQYFLDAYQEGAMLKSSRSNNDVAKCQSCAVAVPSLGVDGTQLVKKSSWSSSLTVSRARQVYQKAAINVDKALVKAEACQQERSCQFLQVPQDEWGGFPLGKDEDIPCRRMRSGSYVKAMAEDDSGDSDGSPKPSPKMQARRASYLKATQPSLTEMTTLNISTEHSPKLQIRSHSYLRAVSEVSINRSLESLDPKALLASPQYRSRNESYMRAMSTISQVSEVEVNGQIEAVCESVFSEMESQAVDALDLPVPGCFRMRSHSYVRAIDQGCSQDDDGPLLPASPPRTTTTVRTIQSSTVSSCITTYKKTPPPVPPRTSTKPFISITAQSSTESAQDAYMDGPGHRGEVTLHSGLSNSTESIDSMKALTAAIEAANAQVHGPASQHVDSSMSTSMPLSMPLPLPVPVPIPVPTLEDNHRDVLRKGRCLSIGIQVDGPEDQEAEDQSKFQSVGVQVEDERSHRRFTRSNSVTAAVQADLDTPDYSPADSPEANILPTGCLSRQYSRDAATSTVSIQGSGNHYHACASDDYEDVGFDPSILPPPDPWIDSVTEDPLEVVSRSVCQRDGRWFLKLLQAETERMEGWCRQMEHDERENELPDEILGKIRSAVGSAQLLMSQKFQQFRELCEENLNPNAHPRPVAQDLAGFWDMLQLSIENISLKFDELHQLKANNWRPLDPPERKERRLPPPVPKKPPKAHPPLARDRSLESTEKQRQEARKRLMAAKRAASVRQNSATESADSIEIYIPEAQTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8J5</accession>
    <name>A0A3B4E8J5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">L3MBTL2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="gene ID" value="ENSPNAG00000019694.2"/>
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    <dbReference type="GO" id="GO:0045892">
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    <dbReference type="InterPro" id="IPR006612">
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        <property type="entry name" value="Znf_FCS"/>
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    <dbReference type="InterPro" id="IPR038603">
        <property type="entry name" value="Znf_FCS_sf"/>
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    <dbReference type="PANTHER" id="PTHR12247:SF64">
        <property type="entry name" value="LETHAL(3)MALIGNANT BRAIN TUMOR-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1"/>
            <end position="89"/>
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            <begin position="174"/>
            <end position="209"/>
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            <begin position="264"/>
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    <feature type="repeat" description="MBT" evidence="13">
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            <begin position="599"/>
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            <begin position="105"/>
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    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="226"/>
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        <location>
            <begin position="793"/>
            <end position="820"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="113"/>
            <end position="129"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
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            <begin position="138"/>
            <end position="153"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="14">
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            <begin position="235"/>
            <end position="260"/>
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            <begin position="710"/>
            <end position="721"/>
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        </source>
    </evidence>
    <sequence length="820" mass="91132" checksum="A973706A932EE429" modified="2018-12-05" version="1">MPNCCAAYGCGKTHKDNVTMFRFPKDPVDFRKWEKQVQRTRRKWFAKGLSFLCSEHFSKDCFEPRSAAVAKTMGFKGLKLREGAVPTIFIRRPCSNCDGSGCMSCSPKKKKQGMSTISTATEPQSTETAEYTGAPLDADPDAAEEESEEEEEENKAHSDEEGVTSPTFGSHSLLARSNEPVVCEMCGISGTRGTFYSRSKRFCSASCSRSFSSNSKKSSVLARLQGRPPARKVHASQTGVSAQTQGTGNHQGSTTSGQDASSGYDWGSYLKKHGYLAASVSSFRHAPLCAQWEDIEVGLRVEVLNAHSALMTKVYWIATIIRLAGYKALLRYEGFENDDSHDFWCNLGSAEVHPIGWCAVNSKLLVPPQALHNHITDWKSYLMKRLVGACTLPVDFHVKLAESMKYPFRQGVRVEVVDRSLVSRTRTAVVDTVIGGRLRLIYEDAGLGPTGEVLSDFWCHMWSPLMHPINWSVRVGHLIKETDKSVDMSSHPTFRKVFCDSVPRQFKKLRTVYVEGGFFEEGMKLEAIDPLNLGNICVATVRKVLLDGYLMVGIDGVEMGDGSDWFCYHASSHAILPPGYCQNNDIPLTLPPGYDQATFTWPKYLEETRSVAAPRRLFNSDPVGHGFAPGMKLEAVDLMEPRLVCVATVQCCVGRLLLLHFDGWESEFDQWVDCESPDIYPVGWCELTGYQLQPPIGPEPKERPNPSKKWMGKKRRRTGRKKTGDDASKATQQGGQVQSDEHPASSHPLEVPSLSKDVPTTMPTMPDVNQLKTEPEEEIISVKVKVEEIEMETPITHNMEDPDIYGTLTPLASLKQESPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E9Z2</accession>
    <name>A0A3B4E9Z2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sphingolipid delta(4)-desaturase DES1</fullName>
            <ecNumber evidence="3">1.14.19.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Degenerative spermatocyte homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Dihydroceramide desaturase-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Retinol isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">DEGS1</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). Catalyzes the equilibrium isomerization of retinols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>11-cis-retinol = 13-cis-retinol</text>
            <dbReference type="Rhea" id="RHEA:55356"/>
            <dbReference type="ChEBI" id="CHEBI:16302"/>
            <dbReference type="ChEBI" id="CHEBI:45479"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:55357"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>11-cis-retinol = 9-cis-retinol</text>
            <dbReference type="Rhea" id="RHEA:55360"/>
            <dbReference type="ChEBI" id="CHEBI:16302"/>
            <dbReference type="ChEBI" id="CHEBI:78272"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:55361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>all-trans-retinol = 11-cis-retinol</text>
            <dbReference type="Rhea" id="RHEA:19141"/>
            <dbReference type="ChEBI" id="CHEBI:16302"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19142"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:19143"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>all-trans-retinol = 13-cis-retinol</text>
            <dbReference type="Rhea" id="RHEA:55352"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:45479"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:55353"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>all-trans-retinol = 9-cis-retinol</text>
            <dbReference type="Rhea" id="RHEA:55348"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:78272"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:55349"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>an N-acylsphinganine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = an N-acylsphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:46544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:31488"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="1.14.19.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:46545"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with RLBP1; the interaction increases synthesis of chromophore-precursors by DEGS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 26">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017539560.1">
        <property type="nucleotide sequence ID" value="XM_017684071.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E9Z2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032119"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021747.2">
        <property type="protein sequence ID" value="ENSPNAP00000032119.1"/>
        <property type="gene ID" value="ENSPNAG00000019886.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412154"/>
    <dbReference type="CTD" id="8560"/>
    <dbReference type="GeneTree" id="ENSGT00390000013448"/>
    <dbReference type="OMA" id="GATCNQN"/>
    <dbReference type="OrthoDB" id="200948at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF2">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017228">
        <property type="entry name" value="Sphnglp_dlt4_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 26" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 26" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 26" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 26 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 26" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 27" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="28">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00030541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00032761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00045712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRNR" id="PIRNR017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="323" mass="38190" checksum="B8B2595DF29DCE6C" modified="2018-12-05" version="1">MGNRVPREDFEWVYTDQPHADRRKEILVKYPEIKSLMGPDPRLKWIVCMMVGVQFLAFYLVKDLEWKWVLFWTYVFGSCINHSMTLAIHEISHNTAFGNNRARWNRWFAMFANLPIGLPYSASFKRYHLDHHRYLGGDGVDVDIPTDFEGWFFCTRLRKFFWIILQPLFYAIRPLCINPKPISQLELANVAVQFTFNAFLYWLWGIKPVVYMLAGSMLGMGLHPISGHFIAEHYMFLKGHETYSYYGSLNLLTFNVGYHNEHHDFPSIPGRRLPLVKKIAPEYYDDLPCYTSWVKVLYDFIMDDELSPYSRVKRKLKGEVKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAI8</accession>
    <name>A0A3B4EAI8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">QSER1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017563491.1">
        <property type="nucleotide sequence ID" value="XM_017708002.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000032251"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021999.2">
        <property type="protein sequence ID" value="ENSPNAP00000032251.1"/>
        <property type="gene ID" value="ENSPNAG00000020097.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433441"/>
    <dbReference type="CTD" id="79832"/>
    <dbReference type="GeneTree" id="ENSGT00440000037417"/>
    <dbReference type="OMA" id="QPYNGTT"/>
    <dbReference type="OrthoDB" id="8447576at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052466">
        <property type="entry name" value="DNA_MethProtect_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025451">
        <property type="entry name" value="DUF4211"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14709:SF2">
        <property type="entry name" value="GLUTAMINE AND SERINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14709">
        <property type="entry name" value="GLUTAMINE AND SERINE-RICH PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13926">
        <property type="entry name" value="DUF4211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4211" evidence="2">
        <location>
            <begin position="1545"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="845"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1089"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1193"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1283"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1491"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="359"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="544"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="845"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1141"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1162"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1299"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1321"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1515"/>
            <end position="1531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1753" mass="191543" checksum="71D389CD0E1C4978" modified="2018-12-05" version="1">MRVCFATLSRGKRTMDRSYPSPGFGEPLAAAGQSPGWAYERPAGGLTPSSSYGATHLETELLHRQTYASTHSLPTYTTSHHPTGLSGVFDTNLHSTGSSTTESSVMNFLSSIESRGLQAGPAATSLLPQFRTPPWQTGTNSSTELFLTGALPSSGTFPTPSALSSFQHPSTFPTRGFSSTSTLTVQDTTFNPSNGLLSPTDPLLQIKSPQSTVPTALAFDRLGSASLAAGLPPQSSTYRSAQESAPHLLQPQFSLLPSALGNSQQASSQPYGAPVFSGSIERALQRECSVIKHHQRPSSTQSVQETLPGSVQHSLQGYLPDDSDVNYQQDPSRHTPVPSSPAGDPTQAVNVNAQQETTSSSSVPSPGFSSSSGVKAKDGSSKITEHPSESTEPHAQTASSGQKQGSVIASQSQPYTSAQLPSLMSISPSQTYITSQSLLSNLSQAQAFSPGQPEKLPSIYKPLPSFASQSENDTPVSQSLIYSSSQQQVLSSAANREDYEAQVQGLCMSNPSQNYSSSHAQGLPAASYYAQGPASGSPSQNYTSGQSLTSSPPFSSSRVHSLPTSSSTQDYILMQPSPGSKSDGLLQSQKYLLSVHSPTSSTATYTQALQKNQTSVEQKQAYGKRKQDETLFLSSKEGCGELPIQDVQALQQASVGTSTQAITNTDIGIQNNIVCVVSKMEDRHNTQSVIRSNSRSEDQIIGLSQMSSVKDERMSTLGQHSQLVSSAHAHVTSVPKNSATLMQPSHVALSTDQLKQHSLLLKAPAVQQENHQTQNNRVQLPDHRQVPQNQTQYVRIPSAQVLMDPTQMILLQQSLLHAGTNQSKPAVQMQPVPIQYLQMNNDIVNSAGSTHGNQQQNHQAAVSAESAKQHLASKDNFSNPSNPHDAKQHFTLNSICFPESILMADERNILSNVDDILAATAAACGVTPQDFKSTSSEGELPTISNAADSKCHFQLVENRHEANSFSTQNMIIANTQTMTLTLNGAQLTANVHKDIGGHQAFTLANSHMQHDNLKQETSDKVTNCHEKNEALPKGQCVNPSNDSSLNSSGGSVNTHNHSEFHLAGQEYNPTGLVKTDNLSKVLNQPKGIKNVKMEDGSMECSSDGLLKKKVRSKGSSKQSGEDENGQPKSQKRTSQAKRQNSRSSETSSSSTSEGYDSYQQQERMRQKIREVEEKQPEVKTGFIGSFLDFLKSGPKQHFSSPPIRSPNRTRKPSVSKRLSNPLSVPLKLLPQSTQLVSPDIHTVSSSKRLDEELQKNLETLPSFSSDEDESVGKNQDLQKSISSALSSLDEPSDKKQKLDSTSSEDFTKQEQPSSTQLTDAKPQELQKPVQKEVSAEELVKNVPPDKLAVQLTTVAIEGLTDEELSDSEGEGMYRERDEFVVKNEEIDSLKITLKAGVEPPAIWKVQKALLQKFIPELRDGKRVFSATNSYLGYFGDAKTMYRRVYVKFLDTVNKREYVRVCNRKPRCKPMHSMRGCQMKALLAHSAAAPAVSDSSSLKPSTKQGLSKPRPKQAKTKAEPPPKKRKKWKEEFTTPPLDSSPEAGSEDDEFTPPVPFATRFLNTRTMKETFRSFVELLISVALDADVMIALERENDELLLPHMKRVDGMITDNRRRLLPKLRVGQVFKNALDSFPELAVVTELKTDGETPTFKVRLSGKAYNRKTMKPSKSPIKLPLEYSVDQQKTHWFSLYHSLQHYKYHTYLMCMEEIALLRSRGVDLGQEETVQKCMSNRAWLEGLFDRFGELLTQVQQACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EID9</accession>
    <name>A0A3B4EID9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017579421.1">
        <property type="nucleotide sequence ID" value="XM_017723932.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EID9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035635"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028939.2">
        <property type="protein sequence ID" value="ENSPNAP00000035635.1"/>
        <property type="gene ID" value="ENSPNAG00000025767.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443339"/>
    <dbReference type="GeneTree" id="ENSGT01030000234592"/>
    <dbReference type="OMA" id="DQPWMYE"/>
    <dbReference type="OrthoDB" id="8572289at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039015">
        <property type="entry name" value="ENDOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN ENDOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472:SF20">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017425482" evidence="1">
        <location>
            <begin position="17"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="3">
        <location>
            <begin position="55"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="2">
        <location>
            <begin position="56"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="288" mass="33421" checksum="900B4725744E5326" modified="2018-12-05" version="1">MKGWLYLLLCLPLARPGVVQDFNHVERCKDSLYMGTPPRGYLNHVYKKICQRLQDRPRYVTLYDPRRRMPVYSAYTFKKSDGEKSVDQPWMYEPQLASGLGSSNMEPFSPSSSSRMLLDSQATLEDFADVVQYERGHLNPDQHQADPVDKAATYALTNVVPQIREFNMGPWAQHEDRIRQRLNNYCRGTAYVVTGTTTAGNMIRRNNNDRVGIPEYVWTAYCCTDFDRNAPYLERYRFPTFGAYGLNDRVNNAVVEVPLKTLEKFLKGRMDVDKNFQIFYNDCIPDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELT4</accession>
    <name>A0A3B4ELT4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Transforming growth factor beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3. Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Latency-associated peptide: Homodimer; disulfide-linked. Latency-associated peptide: Interacts with Transforming growth factor beta-1 (TGF-beta-1) chain; interaction is non-covalent and maintains (TGF-beta-1) in a latent state; each Latency-associated peptide (LAP) monomer interacts with TGF-beta-1 in the other monomer. Transforming growth factor beta-1: Homodimer; disulfide-linked. Transforming growth factor beta-1: Interacts with TGF-beta receptors (tgfbr1 and tgfbr2), leading to signal transduction. Interacts with EFEMP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19 21">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017544934.1">
        <property type="nucleotide sequence ID" value="XM_017689445.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELT4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036141"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030107.2">
        <property type="protein sequence ID" value="ENSPNAP00000036141.1"/>
        <property type="gene ID" value="ENSPNAG00000026649.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108416490"/>
    <dbReference type="CTD" id="559723"/>
    <dbReference type="GeneTree" id="ENSGT00940000166208"/>
    <dbReference type="OMA" id="WSANNHY"/>
    <dbReference type="OrthoDB" id="5949851at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005160">
        <property type="term" value="F:transforming growth factor beta receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000004">
        <property type="entry name" value="Transforming growth factor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000006">
        <property type="entry name" value="Transforming growth factor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016319">
        <property type="entry name" value="TGF-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003940">
        <property type="entry name" value="TGFb2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF249">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001787">
        <property type="entry name" value="TGF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01423">
        <property type="entry name" value="TGFBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01425">
        <property type="entry name" value="TGFBETA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 19 21" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11 19" id="KW-0497">Mitogen</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 19" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017106917" description="Transforming growth factor beta" evidence="19">
        <location>
            <begin position="18"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="22">
        <location>
            <begin position="299"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="318"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="347"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="351"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="20">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR001787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001787-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="415" mass="47437" checksum="10FB920508964192" modified="2018-12-05" version="1">MWFINLILLVLKLSASAEGFSTCQSYDLDEHKSKRIEAVRGQILSKLRIRSPPDPASAPRPDAVPLEVMLLYNSTKELLKERARHAEAACDRESSEEDYYAKEVQRVDMLPQRADNNAINPQPLSPYFRVVRFDVTNVERNSSTLVKAEFRIFRVQNARARATEQRVEIYQILKSDEVTGPSQRYIDSRTVQPQAKGAWLSVDVTETVKEWMAFRERNLGLKLSVHCPCCTFVPSTNNIVPNKSEELEARFAGVDDDLMRQSRRPGATKGQIEFSTKTPHLILTLLPTDRLDNPAKKNRKKRAAADTTTCSRNADQGCCLRSLYIDFRRDLNWKWIHEPKGYRANFCAGNCPYLWSADNHYNMILPLYNKMNPEASASPCCVPQDLEPLTIVYFLGRTPRVEQLSNMVVKSCKCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EPT8</accession>
    <name>A0A3B4EPT8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FBXO31</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FBXO31 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555606.1">
        <property type="nucleotide sequence ID" value="XM_017700117.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EPT8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037294"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033259.2">
        <property type="protein sequence ID" value="ENSPNAP00000037294.1"/>
        <property type="gene ID" value="ENSPNAG00000028889.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428814"/>
    <dbReference type="CTD" id="79791"/>
    <dbReference type="GeneTree" id="ENSGT00390000001368"/>
    <dbReference type="OrthoDB" id="722566at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22102">
        <property type="entry name" value="F-box_FBXO31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000042">
        <property type="entry name" value="F-box protein 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF130">
        <property type="entry name" value="F-BOX ONLY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12014">
        <property type="entry name" value="Cyclin_D1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="78"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="420"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="564" mass="63478" checksum="20AEE46B2FA77B6F" modified="2018-12-05" version="1">MAVCARLCGVGQSRRCRRRQRHGQHDQHGSDSEMDMDEEEGEKIVGETRGSGSGGGWPQSRATGPSEAAAASAGTQSPQSLLDLPPELLVEIFSTLPGIALPNLALVCKKFRQILSTETIWRRRCIEEFGMKDDLRKMEVVGVSSRELYVKRVNPRVKSGRFMKLLPDYEHMDYRDVYTHLLHPYRHILGLWQPDIGPYGGLLNVVVDGLFIIGWMYLPPHDPRVEDPMRRRPLFRIHMWERNKATVECMYGHKGPHKGDIQTVKKDEFSTKCNQTDHHRMPGGRQEEFRTWLEEEWGRTLEDIFHEHMQELILMKFIYTSQYDNCLTYRRIYLPPCLPSDLLQPGLFKGTYGSHGLEIIMLSFHGSRAKATKLTGDPNVPAGQLTLDIDLSHSVQLPDLEHQRSIEELSRLVMGVHEEVQRGSQGPAAASANAEEGSSGAEGSDATPACDAEAGEAGAGAASSSPESQPFILPLGVMARNEVYPRTCKMCFYGTGLIAGHGFTSPERTPGLFVLFDDDRFGFIWLELKSFSLYSRLTDQLEHAQAPSMERFEAMLRNMQSWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IGR1</accession>
    <name>A0AAR2IGR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MOB-like protein phocein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mob1 homolog 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Mps one binder kinase activator-like 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Preimplantation protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TRIM16</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
            <location evidence="5">Golgi stack membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MOB1/phocein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IGR1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059024.1">
        <property type="protein sequence ID" value="ENSPNAP00000038325.1"/>
        <property type="gene ID" value="ENSPNAG00000008244.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271909"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000002">
        <property type="entry name" value="MOB-like protein phocein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="260" mass="29674" checksum="58BFD8D5272B3B55" modified="2024-11-27" version="1">LAKAFRKFLPMFDRVLVERLAAETVTKGGIMLPEKSQGKVLQATVVAVGPGAVNKDFYNWPDESFEEMDSTLAVQQYIQQNIRSDCSNIEKIMEPPEGQDEGVWKYEHLRQFCLELNGLAVKLQSECHPDTCTQMTATEQWIFLCAAHKTPKECPAIDYTRHTLDGAACLLNSNKYFPSRVSIKESSVAKLGSVCRRIYRIFSHAYFHHRQIFDSYENETFLCHRFTRFVVKYNLMSKDNLIVPILEDEGQNTSAGESEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IIM4</accession>
    <name>A0AAR2IIM4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSPNAT00000085111.1">
        <property type="protein sequence ID" value="ENSPNAP00000039455.1"/>
        <property type="gene ID" value="ENSPNAG00000035085.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000290361"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="12"/>
            <end position="36"/>
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        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="103"/>
            <end position="126"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="136" mass="14799" checksum="DF9AFEB36FFCAFA6" modified="2024-11-27" version="1">MTILFKAILSEAVLSMTVLFKAILSEAAVLSMTVLFKVILSEAVLFMIVLFKAILSEAVLSMAVLFKAILSEAVLSMTILFKAILSEAVLSMTVLFKVILSEAVLFMIVLFKAILSEAVLSMAVLFKAICLRLCCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0AAR2IJ83_PYGNA</name>
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        <recommendedName>
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        </recommendedName>
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        <name type="scientific">Pygocentrus nattereri</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJ83"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="18"/>
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            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="Ensembl" id="ENSPNAP00000039638.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="44176" checksum="F9CEC8158E6FCD50" modified="2024-11-27" version="1">MRFVLLCLSFVRFSHLWGNPMSLEGNYSLECLNDYLFTITCVLNVSSNLLPHGATSWLEFNDYREPYQCVLKVQAHSWVCELDLTPLVEYTFTDTDFFQISLNSSYHGNSSSAVLEVLYRPVKHIRPVPPSNLSYLGKTDRVVFHWLSGYQENTSFVQNLQYELSIHGDGKVTDVHPVDTHVVVDMSIFVPHRSYMARVRSVPNQMHYRGVWSHWGPAVHWTTGANSKGETPGSSFSSIWFAVFCLLPVLFLLLSYIPYYRWKKQVLIPSPTPYIRDFKRCAVPLRIVGELLQGEESLKIDSMIEESDSTPSPNPVHYEKMRSVHDQSEQPFITSAAPFTSPSMIRSPAGAEISFSSWLEDCAATDTGSVTCSEDYCTLSHINTEQANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IP94</accession>
    <name>A0AAR2IP94_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
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        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
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        <property type="gene ID" value="ENSPNAG00000034870.1"/>
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        <property type="component" value="Chromosome 18"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="6"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Protocadherin alpha 2"/>
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    </dbReference>
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        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
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        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF236">
        <property type="entry name" value="PROTOCADHERIN GAMMA-C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
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        <location>
            <begin position="30"/>
            <end position="893"/>
        </location>
    </feature>
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        <location>
            <begin position="691"/>
            <end position="715"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="134"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="243"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="351"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="456"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="580"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="893" mass="98936" checksum="A6C6820A6F53D252" modified="2024-11-27" version="1">MTERRGSGSWKRHLFWRFLFLLLWNKAEAQTRYSVPEELNVGSVVGNIAKDLGLKISELYDRKLRIASESGKQYFSVDLGKGEMVVHERIDRERLCGQNANCLLPVQVIIEDPLQLHRIEVEIQDINDNYPHFLSADRSLKIAESTVPGMRFPLESAQDPDVGSNSLKSYTLSKDECFSLKVKDLGDGRKIPELVLEKALDREKKAVHQLLLTALDGGNPVRSGTTQINITVLDNNDNNPTFDKTVYKVNIPENSIKGTTVVKVVATDLDEGPNGEVEYSFGEHTAEAVRSLFYIDSKTGDFILNGELDYETTSTYNIEVSAKDKGMPEMEGHCTVQIDVLDVNDNSPQIVLTSKPSPVREDAPSGTVVALISARDVDSGANGKVTLQTQPNVPFILKPTFSNDYSLVTNGVLDREIFPEYKVEVTAFDSGSPSLSSKKTIPVQILDVNDNPPHFTDRAYKVYVKENNNPGSIICSVSASDLDVGENAKLSYSIIDSKAQDVPLSSYVYINSDNGSIYTMHSFDYEKMKVFQIQVQVKDHGSPSQSSNATVHVFILDQNDNAPAVIYPSAVMGSASHQRMPRSAKAGHLVTKVTAVDADSGHNAWISYRLAEAADASLFSVNLHTGEVRTKRSVSEQDDSSQRLLIEIKDNGEPVQSTTVTVDVLIEDGFHEPVSDYRKKSAEVSKKSGKITLYLIISLASVSLLCLMTFFILLVKCARGSRGSSSCCIRRSESGYKNPNRNLQIQLNTDGPIKYVEVLGGDMMSQSQSFGSYLSPMSEFSDFTLVKPSSTTDFTDTLNVLDASLPDSTWKFESQQVSREVQFKFFFVTFALWRAHAACALKSKLQNFYIPWLKNPNQGLPSATCPRIWAWTFGAWFTGSCALFRNLTPNIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ISD6</accession>
    <name>A0AAR2ISD6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2ISD6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045457.1">
        <property type="protein sequence ID" value="ENSPNAP00000042625.1"/>
        <property type="gene ID" value="ENSPNAG00000032334.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043927452" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="23"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="622"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="654"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="698"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="743"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="778"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="810"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="584"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="849" mass="95206" checksum="013A136184588CB3" modified="2024-11-27" version="1">MESIFTLLNVVMTILNFSSTNGTFCSLQGEPVYPQLSKDGDITIGGIFPFHRTWEMTDVSYMVKPSPLNCMSVDFRTIQFSQSLIFAIEEINNSSSLLPGVSLGYKMYDTCSSTAMGVRMALALINGNENSALNEQCTKPAQVQAIIGETYSSVSMAIAKSVGPFSIPLISHDSTCECLSDKRIYPSFLRTVPSDYYQSRALAEMVKQFGWTWVGAIRRDDDYGNSGMAAFTKAAEQLGICLEYSLPFFKTYSREKVLRIIQQIKSSTSRVIVAFVGHWDLEILLNVFYEHNITGYQWVGTESWISDSTVARLDKHKILQGAIGLALAQTTVTGLKDFILDIYPLKSAGRAIFTEFWEALFNCKFIMQNDSDDTTVCTGQEKLFETENAFTDLSLMPMFCNVYKGVYAVAHTLHKLLSCTQTCPTKKQPDPLTFLEHLKRVHFKTKDGEEVYFDNNGDPVAKYEIINWQTTDDHHEFVTVGLYDSSLPENNRLAIKIASIVWTQNTNQVPVSVCSESCPPGTRKAVQKGKPICCFDCIPCAEGEISNMTDAIKCEQCYQDYWSNTPRNECVKKEIEFLSYEETMGILLTTVSIIGTFITIIIGIIFFKHKNTPIVKANNSELSFLLLFSLALCFLCSLTFIGRPSEWSCVLRHTAFGITFVLCISCVLGKTIVVLMAFRATLPGSNVMKWFGPLQQRLSVLAFTLIQVLICVLWLTTSPPFPFKNLKHYKEKIILECHLGSAIGFWAVLGYIGFLAILCFILAFLARKLPDNFNEAKFITFSMLIFCAVWITFIPAYVSSPGKFTVAVEVFAILASSFGLLLCIFLPKCYIIIMKPEKNNKKQIMGKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITE9</accession>
    <name>A0AAR2ITE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566250.1">
        <property type="nucleotide sequence ID" value="XM_017710761.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ITE9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044442.1">
        <property type="protein sequence ID" value="ENSPNAP00000040896.1"/>
        <property type="gene ID" value="ENSPNAG00000030432.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108435144"/>
    <dbReference type="GeneTree" id="ENSGT00940000164807"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000226">
        <property type="entry name" value="Zinc finger protein 236 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF66">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="333" mass="38228" checksum="468D649BB7CA6D13" modified="2024-11-27" version="1">MDAAANMAANKFEFLNSFVSQRLLAVAGEIFQVVKETINEYQEEIERTTQENHYLKKMLTEKCTSNGADNETSPAVPQELTNFNQESMDSVIQVKVELSTMHQDAESQGPLNNPSSSSSPSAVTAYQEPSYHFSENTTNKEEVADDLHNSGVTVKLEPWDSQVIYSDDAPSAMQNEIIANNNFEGMRDDQGTSYSQCQVTLESMSHAEPRIQSQTVERVVYCEYCGKPFGNREQLKQHLVVHQKEKPRPYRCDLCEKSYSYAQVLEIHRRTHTGERPYHCRFCGRRFNQQSHLKYHERIHTGERPFGCSLCGKRFIQRSQVKKHNCKRQVILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IUC2</accession>
    <name>A0AAR2IUC2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Growth factor receptor-bound protein 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GRB7/10/14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IUC2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069863.1">
        <property type="protein sequence ID" value="ENSPNAP00000041642.1"/>
        <property type="gene ID" value="ENSPNAG00000035647.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155909"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:insulin receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PH_APBB1IP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH2_Grb10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000062">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000056">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000015">
        <property type="entry name" value="Growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015042">
        <property type="entry name" value="BPS-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035037">
        <property type="entry name" value="Grb10_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039665">
        <property type="entry name" value="PH_APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
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        <property type="entry name" value="RA_dom"/>
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    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF4">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08947">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Ras-associating" evidence="9">
        <location>
            <begin position="179"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="305"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="7">
        <location>
            <begin position="508"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
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            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="69895" checksum="1111A910878EBF20" modified="2024-11-27" version="1">MNNFALNCCFNWILHKLMNYWMFKQEQDNVDRTANHRPAELIGSSFQQAANRNSPNHQEDDVDLEALVNDMNSSFESLCSTYNVQAEAVPLLHNGQSHRPNEQTHSHSQAHGQLTSLHQKLRRSQPMHILAVSRRLQVEEEQYRTSSLPAIPNPFPELCSPASSPTLCPGSLPPSQPSGKYIIKIFNEDGMVKVVEILANMTARDLCQLLVYKSHCVDDNSWALVEHHPAMGLERCLEDHELVMQVQASMTSESKFLFRKNYAKYEFFKNPLNFFPEQMVAWCQESNRSIPPSQLLQNFLNSTSCPEIQGYLYMKETGRKSWKKLYVLLRRSGLYFSTKGTSKEPRHLQLLADLEDSNIYTVIAGKKLHNAPAEYEFCIKPNKVRSEQKEMRMFCAEDEQSRTCWMTAFRLFKYGILLYQNYKIPQQRKALLSHFSTPVRSVSENSLVAMDFSGRIGRVIDNPVEAQSAAVEEGHAWRKRSQRMNNLGSPSPLHPSSLSGVIHKTQLWFHGRITREESHKMILQQGQVDGLFLLRDSQSNPKAFVLTLCHHQKSKHFQILPCEEDGQVFFSLDDGTTKFTDLIQLVEFYQLNRGVLPCRLKHPCTTVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IUW0</accession>
    <name>A0AAR2IUW0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">DAZL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017539820.1">
        <property type="nucleotide sequence ID" value="XM_017684331.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IUW0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051125.1">
        <property type="protein sequence ID" value="ENSPNAP00000043518.1"/>
        <property type="gene ID" value="ENSPNAG00000034292.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108412351"/>
    <dbReference type="CTD" id="1618"/>
    <dbReference type="GeneTree" id="ENSGT00530000063480"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070935">
        <property type="term" value="P:3'-UTR-mediated mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
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    <sequence length="217" mass="24247" checksum="390762E6AF0E0FE6" modified="2024-11-27" version="1">MDIQKQRHGFQSSALKLSNGYVLPEGRLTPNTLFVGGIDMKVEENEIRDFFARYGAVKEVKIITYRGGICKGYGFVYFSEDVDIQTIIEQQITFKGKKLKLGPAIMKDRSYRESGSYQSPLVGPSQWVSPSPYMYCTCCSPGLASPSPVLSGSSQYLQPYSYTGFPGVVIPQVPVNYAQSSYAYPYPIPPQWCGDQRTRLINQNYVDCGVQTLLTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IVJ3</accession>
    <name>A0AAR2IVJ3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Collagen, type XIV, alpha 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037398349.1">
        <property type="nucleotide sequence ID" value="XM_037542452.1"/>
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    <dbReference type="Ensembl" id="ENSPNAT00000063161.1">
        <property type="protein sequence ID" value="ENSPNAP00000043715.1"/>
        <property type="gene ID" value="ENSPNAG00000022703.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435589"/>
    <dbReference type="CTD" id="100331704"/>
    <dbReference type="GeneTree" id="ENSGT00940000153769"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005614">
        <property type="term" value="C:interstitial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="8"/>
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        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
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        <property type="entry name" value="VWF_A"/>
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        <property type="entry name" value="COLLAGEN ALPHA"/>
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    </dbReference>
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        <property type="entry name" value="COLLAGEN ALPHA-1(XIV) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
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        <property type="match status" value="8"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
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    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="VWFA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043815110" evidence="12">
        <location>
            <begin position="30"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="33"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="13">
        <location>
            <begin position="158"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="357"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="447"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="539"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="626"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="736"/>
            <end position="826"/>
        </location>
    </feature>
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        <location>
            <begin position="827"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="13">
        <location>
            <begin position="1034"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1453"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1648"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1490"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1571"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1658"/>
            <end position="1667"/>
        </location>
    </feature>
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        <location>
            <begin position="1669"/>
            <end position="1678"/>
        </location>
    </feature>
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        <location>
            <begin position="1724"/>
            <end position="1735"/>
        </location>
    </feature>
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        <location>
            <begin position="1748"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1771"/>
            <end position="1780"/>
        </location>
    </feature>
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        <location>
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            <end position="1808"/>
        </location>
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        <location>
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            <end position="1826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1855"/>
            <end position="1867"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049648"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50234"/>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043715.1"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1867" mass="200958" checksum="564D8F3A5970632D" modified="2024-11-27" version="1">MMRTKMQARSSSMLFLLALASLLTGPTQGQAPAPRRLRFKELGSSMLSVSWKEPKGEFDSYRLIYSSGHQEREVQVTKEEAKFVISDFDHSKEYTFKIIALNGGQQSKPLLGSYKASRSEVTDSSLQTHRGKQNSEPERDNEISEVDVFVCKTPAIADIVILVDGSWSIGRINFRLVRMFLESLVNAFSVSSDQTRIGLAQYSGDPRIEWHLNTHSTKDAVINAVKNLPYKGGNTLTGLALMFILENSFKPESGSRTGVPKIGILITDGKSQDDVQGPARSLREAGIELFAIGVKNADENELKAIASPPSETHVYNVADFSVMSSIVEGLTRTVCGRVEQLNKEIHGEGGPSTIPAAPRDLVTSEVTARSFRVSWTHAPGTVEKYRVVYYASRGAQPDEVVVNGDQNSVVLQHLNSLTEYQVAVFAVYSNSASEALRGSVTTLALPMVNNLELYDITHSSMRVQWTAADGASGYMILYAPLTEGDAADEKEVKVADTVTEIELQDLTPATEYTVTVYAMYGDEASDPRTGQETTLPLTPARSLQISDVSHSSAKLTWEAASRKVNGYRIMYVKTDGVQTNEIEVGRITTLLLSNLTSLTEYTVAIFAIYDEGQAEPATDSFTTKSVPSPWSFRSSDVGTDRFRVSWQHAASDITLYKLTWMPASGGETRESVISGNSNSYVITGLTPLTEYEVALTAIYRDERESDALELSQTTAARTTTVATTTTTTTTAVIRLGVRGLQLSDFTTFSMGVSWELLGSNVRQYRVSYVTARGDRAEQVTMVPGNQNSVVLQPLLSDTEYRVSVTAVYADEDGPSVTRSARTLPLSAPKNLRVSEEWYNRFRITWDTPPSPTMGYRIVYQPVSAPGRALETFVGDDVNTILILNLLSGTEYSVKVIATYTTGSSEALTGRARTLYLGVTNLNTYQVRMNSMCAQWQPQRHANQYRVVIESLLNGQKQEVQLDGSSSRHCFNDLSPNTQYKITVYAQLQDTEGPAVTTIQKTFPVPTTPPTRPPTTTPLPTIPVAKEVCRAAKADLVFLVDGSWSIGDDNFVKIIRFLYSTAGALDEIGPGGTQVAIAQFSDDARTEFKLNSYDNKESLLDAIQRISYKGGNTKTGRAMQHVKDSIFTVAGGSRRGVPKVLVVLTDGRSQDDVNKISQEIQMEGYIVFAIGFADADYGELVSIASKPSERHVFFVDDLDAFRKIEEKLITFVCEAATATCPSVPMSGSTLPGFRMMEMFGLVEHLYSNLEGISLEPGTFNSFNSFRLGSDALLAQPTRYIHPEGLPSDYTITMVFRLLPETPQEPFALWEVLNSRNEPLVGVILDNAGKTLTFFNNDYKGDFQTVTFEGPEIKKLFYGSFHKLHIAVSKTLAQVMVDCNLAGEKPINAAGNITTDGLEVLGRMVRSRGDKDNSAPFQLQSFDIVCSTSWARRDKCCDLPSLRKEADCPALPRACTCTQDSKGPPGPPGPTGGAGMRGAKGDRGEPGPVGPVGPMGDAGIPGPQGPPGPQGPSGRSIRGPPGGPGERGEKGEAGARGLQGVPGTPGSPGREGPPGPRGLQGKDGPQGRVGPPGSVGGPGAPGAPGQNGPPGPIGEQGPPGPAGSKGEKGERGDMQSTSSVQAIARQVCEQLIQSHMARYNSILNHVPSQPVSIRTVAGPPGEPGRPGPPGQQGEQGSPGRPGFPGTNGENGQPGSRGPPGEKGEKGSPGVGIQGPRGSQGPPGPPGEGRAGGPGPAGRPGNPGTSGRPGMPGPVGPAGPPGYCDPNACGYNVGEREIDREVTDSDIPVVQLAPNPYQPYTPEEEGEEEDPYGSYGTYPSYYQPNYPQPRLVEPGDPVIAEDTEELRSPGIQRQTRSVAERRRGQKGNDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J9E1</accession>
    <name>A0AAR2J9E1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">IGKC</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J9E1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086950.1">
        <property type="protein sequence ID" value="ENSPNAP00000046942.1"/>
        <property type="gene ID" value="ENSPNAG00000034999.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="119" mass="12804" checksum="270267FBCDE3954E" modified="2024-11-27" version="1">WTFGGGTKLSVGSVAAPTLTVLPPSSVELQQGKATLVCLANKGFPSDWRLSWKVDGSSWSSGVSQSPGLLQKDNGLYSWSSTLTLQEDQWLKKTVTCEATKDSQPAVSQTLRREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J9M3</accession>
    <name>A0AAR2J9M3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017552689.2">
        <property type="nucleotide sequence ID" value="XM_017697200.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037387117.1">
        <property type="nucleotide sequence ID" value="XM_037531220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J9M3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045242.1">
        <property type="protein sequence ID" value="ENSPNAP00000048738.1"/>
        <property type="gene ID" value="ENSPNAG00000023835.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427211"/>
    <dbReference type="CTD" id="561247"/>
    <dbReference type="GeneTree" id="ENSGT00940000159004"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09945">
        <property type="entry name" value="SH2_SHB_SHD_SHE_SHF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000021">
        <property type="entry name" value="Putative SH2 domain-containing adapter protein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051846">
        <property type="entry name" value="SH2_domain_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127">
        <property type="entry name" value="HEAVYWEIGHT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127:SF33">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING ADAPTER PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="295"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="395" mass="44645" checksum="DE8399682726FBD8" modified="2024-11-27" version="1">MAKWLKDYLSFGSRKGPPQPPKPDYTESEILRAYRAQKELDFEDPYEPSDNRPQNGQGAADPFPAFGTVLPNGVEVKIVSPKHRLIKVESQEFGRCKIPLSIVSFEEPHAPVVPSAPAVGDTDYSDPFDARPDQRAQPAWDHSSVENSSSYMEPFEAQRVITELQHSSEPSRSGSRGSPQLYDSPYEERSRHRAAASQDDERGSRLPQDDERPADEYDQPWEWKKENISKAFAVQFDGGDWDRSTLPHVEQQRRAGASRTSPPSSKATSLRKPGDPHTILGERVNPTLPLDQQVWYHGALTRSEAETLLTLCKECSYLVRNSQTSRNDYSLSLRSCHGFMHMKFSLSRDGKYILGENSPPFDTIPEVIYYYTTHKLPIRGAEHLSLLFPVVVQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JAA8</accession>
    <name>A0AAR2JAA8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LisH domain-containing protein ARMC9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JAA8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049194.1">
        <property type="protein sequence ID" value="ENSPNAP00000047164.1"/>
        <property type="gene ID" value="ENSPNAG00000010732.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018026"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040369">
        <property type="entry name" value="ARMC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048959">
        <property type="entry name" value="ARMC9_ARM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056327">
        <property type="entry name" value="ARMC9_CTLH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048957">
        <property type="entry name" value="ARMC9_LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14881:SF4">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN ARMC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21050">
        <property type="entry name" value="ARMC9_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21051">
        <property type="entry name" value="ARMC9_LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23138">
        <property type="entry name" value="CTLH_Armc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="621"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="655"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="ARMC9 CTLH-like" evidence="11">
        <location>
            <begin position="51"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="LisH" evidence="10">
        <location>
            <begin position="435"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="846" mass="93890" checksum="21ED4AE9162C4074" modified="2025-10-08" version="2">MGTILPHSALHYLKFCEFEETAKIFEKECKSKGKPVPKTQSSPPADSRSLIIQKDLISSFEDGDMKVFFELWAEHVPTDIRDSDPAVQKLEFYLHIHFVIYPLKNSLGRQDKAELDERISHFRHYLETRGAALSQTTEFLPFYALPFVPNPAAHPSFQELFQDSWAPDLRKRLEKLLSLILKASATPRLLTLYNLKQTQAQLAEAEKRASCYMRRFSKMQADYHNLIGVTAELVDSLEATVSGKMISPEYLQGVCVRLFSGQMRQSAAQSIDFTRPGTASSMLRASIAAPKDVPLLPSLDYEKLKNDLMTGSDRLKALLLQALRWRLTRSLQGEQRDTVLQAYISNDLLNCCHTKQKTVLHLVKSKNEIVRQYTARLINAFASLSEGRMYLSQFPPLLKLLVDCLHTEEKDSVARENVLGALQKLSLRRVHQSTLIRQGLIGWLVDELQDSDSLSDYTLEYAVALLMNLCLRTPGKRRCTERAKHVLKVLTDLLGHENHEIRPYVNGALYSILSVPTIRLEAKEMSMEEILHCYTKEENPELNRQIEFIIKQLNSEEEPQDELESDDEEDEDGDDEEDVMEADLDKEEVLQPQPKELSGENLLTSEYLGVSPQTTSLSRSLPLSLLLCLSLSLSLSLSLSLSLSLSLSLSLSRSLPLSLLLCLSLFLSLSLSLWLSLSLSLSHLSFSLSLSRSLSLSLLLCLSLSLSLHPSLSLSLSLSLSPSLSLISLSLSLSPALCLSLCFSASLSLSLSIPLSLSPSLCLISLISLSLSRSLSLSLLLCLSLSLSSLSPSLSLISLSHISLSLSLSLSLSLSLSLSLSLSHSLSLSPALCLSLPLSVSLPGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JAM8</accession>
    <name>A0AAR2JAM8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JAM8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049496.1">
        <property type="protein sequence ID" value="ENSPNAP00000048980.1"/>
        <property type="gene ID" value="ENSPNAG00000031602.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000279840"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="171" mass="20881" checksum="CDA21239DC302277" modified="2024-11-27" version="1">LNTLQCWNDTLQCWNDILQCWNDTLQCWLNTLQCWNDTLQCWNDTLQCRLNTLQCWNDTLQCWLNTLQCWNDTLQCWNDILQCWNDTLQCWNDTLQCWLNTLQCWNDTLQCWNNTLQCWNDTLQCRLNTLQCWNDTLQCRLNTLHCWNDTLHQHCTTFNQYHKVLFQHSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JC42</accession>
    <name>A0AAR2JC42_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4174 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JC42"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061588.1">
        <property type="protein sequence ID" value="ENSPNAP00000049603.1"/>
        <property type="gene ID" value="ENSPNAG00000032439.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166240"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010811">
        <property type="term" value="P:positive regulation of cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025232">
        <property type="entry name" value="DUF4174"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46792:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 80-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46792">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13778">
        <property type="entry name" value="DUF4174"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043703338" description="DUF4174 domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4174" evidence="4">
        <location>
            <begin position="62"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4174" evidence="4">
        <location>
            <begin position="375"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="535" mass="60214" checksum="BC02AE664A36357C" modified="2024-11-27" version="1">MHHLWIVLTGLVLWSCSTALSARTGKNRQRSKAKDEPVGPREHAGADEANEREPEGASGTDFLADFAGKKRLWVITAPSYSDNYLRMMQQQIQESEGLNCRLAERDTLIVTIIQNAMMEGKIRHTTMQGQATEEAMDPDMVTKLLHYLEVEHQTFSMLILKKNLKVSERFPYPVRVEAVLEVIDQLPVRKLEKITRKGLVQRCKITKKRLVVKMSNPNKRRLFSSQKQGNVTSVVPSPQQAADKKAVIRDKVKDILSGRFRFVIRKKPESSSGGKSSNATKSSANEEVKEPESQKESTEGPDKVGNKRDSASKVDKGNDKVSSGVFTVDEDSKGEQKVENSKSKKKGKGKKKKGKRGGKASQRDSKDKAALKEFIEKLKGKRRLLVISSPSDVSSQYIQQRDENELHSCDFALRKVTVLAILGSEHNPTLRLQHYQQDGDPQLASLPDNFKNPDLISQIRKEYGLVSRQYSMVVTDYDMKPNLQKVFDLPTAPSNIINYIDNLQSRQLEKEQEKKAPSPCAPSEKNTEAENSLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLR5</accession>
    <name>A0AAR2JLR5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLR5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087944.1">
        <property type="protein sequence ID" value="ENSPNAP00000052950.1"/>
        <property type="gene ID" value="ENSPNAG00000025960.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156124"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15523">
        <property type="entry name" value="PHD_PHF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24102:SF6">
        <property type="entry name" value="PHD FINGER PROTEIN 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="596"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="516"/>
            <end position="591"/>
        </location>
    </feature>
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        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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            <begin position="379"/>
            <end position="389"/>
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        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="544"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="672" mass="72116" checksum="8468DBFBA03E9B9C" modified="2024-11-27" version="1">MVFKTADVVKAEGGVGFGQKGLMMELQSLQEALKVEIQIHQKLVAQMKQDPQNAELKLQLHDLQAKITALSERQKKVVEQLRRDLLVKQESAEIRLQTQTQLPLQTEGKTTTLVSSQSQSLPPPHLSAPQNSVSVTPVIATKTLPLVLRAATPTTPIAMMPTPTIAMVTTLTNTPLSSLPNSDPQSSPISLQSCSKPVNQGTEPVRIVPKNTIVVQAATQPIKVPQFVPPTRQTARPASLPQVMPKPPASVTPTALQPILASPQSLQQPVLLAAKLSSALLPSSGPVHQLHIFSPQPSATVTQPCPINAQPGPTVTPAITQPCPSNAQLSPNTIQPCPSITMTLNTNISQLNTSSTLHNSTHLPSIIISPKPTQVQAASSIQLSSNSTSTDIHHAARSSNPCPDALTPVAVDGTEPKTSSNSPTPLSATLQSPAPASTSSSTLTQTNTPAASHTHTSKRQENPLKLAYMVSLGLVTHDYLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSTLSYLNSANQGCRKRGRPPKYSIAVERGDSPLTPTSRHPASLGPQQPSMGGPHSPALPQTLSSYPPPSSPRVSPSSERDGDIRDDFCVVCRRSGQLLMCDTCSRVYHLDCLEPPLRTVPRGMWICPSCQSQILKKDEAIEWPGTLAIVHSYISYKAGNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JRM3</accession>
    <name>A0AAR2JRM3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inhibitory synaptic factor 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">INSYN2A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JRM3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053676.1">
        <property type="protein sequence ID" value="ENSPNAP00000052932.1"/>
        <property type="gene ID" value="ENSPNAG00000036387.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063787"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060080">
        <property type="term" value="P:inhibitory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029337">
        <property type="entry name" value="INSYN2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28682">
        <property type="entry name" value="INHIBITORY SYNAPTIC FACTOR 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28682:SF4">
        <property type="entry name" value="INHIBITORY SYNAPTIC FACTOR 2AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15265">
        <property type="entry name" value="FAM196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="50051" checksum="C23E95CFD97EF675" modified="2024-11-27" version="1">MVSKEAGRCALSNSESDSECAGPTLGEEGGGRGDAVRKRNKALQVRFKDICEAQNEAARLARGTRGRSVSCKVVHRRYMTMPARRSIPNVTKSTAVQTSPDLKKRYQTFPFERKKGHTIKHTALVENYQGQNNGFLSDVKGTEEERPCGGKIQKTRVLLHSMPPCSDVQDLCAGSDCPDSDLCPDPSCRGRDANTNSDQTDSRKEGSSQGSKHNEGEVGAGQERNCAKSSSSTSSTQPLQDRGSTSKVTGPIAWTSLTQVECLESPSVRCKRKKETAQINGLQSQTIPHAASCSSQEATQAHYHGSHAFGSTATREDVAGQGYGQVIPLPGAEGDVKARLQAMETLISSSQETIKVLLGVIQELEKGEAQREGRLSYRTGQDTANCDTCRNSACIIYSVELDFKLQEDKLQPLMKRLCPTVDPPYPSLPYSHEVITSTPKRKSKSEAKKHARWKLWFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JUL0</accession>
    <name>A0AAR2JUL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycoprotein hormones alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CGA</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycoprotein hormones subunit alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JUL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087217.1">
        <property type="protein sequence ID" value="ENSPNAP00000055758.1"/>
        <property type="gene ID" value="ENSPNAG00000008318.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012242"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016914">
        <property type="term" value="C:follicle-stimulating hormone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016913">
        <property type="term" value="F:follicle-stimulating hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010893">
        <property type="term" value="P:positive regulation of steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006590">
        <property type="term" value="P:thyroid hormone generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000011">
        <property type="entry name" value="Glycoprotein hormones alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000476">
        <property type="entry name" value="Glyco_hormone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11509">
        <property type="entry name" value="GLYCOPROTEIN HORMONE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11509:SF0">
        <property type="entry name" value="GLYCOPROTEIN HORMONES ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00236">
        <property type="entry name" value="Hormone_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00274">
        <property type="entry name" value="GLYCOHORMONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00067">
        <property type="entry name" value="GHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00779">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00780">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50277">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0372">Hormone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043113375" description="Glycoprotein hormones alpha chain" evidence="7">
        <location>
            <begin position="25"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="128" mass="14474" checksum="76ADC45747EBCA7B" modified="2024-11-27" version="1">MILMIKSAGAIILLLSVLIQTGQLYLNNDVTNCSIVHWYFQFGCEECKLKENNIFSKPGAPVYQCMGCCFSRAYPTPLRSKKTMLVPKNITSEATCCVAKEFKRVFVNDVKLVNHTDCHCSTCYHHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K515</accession>
    <name>A0AAR2K515_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
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        <property type="protein sequence ID" value="ENSPNAP00000057211.1"/>
        <property type="gene ID" value="ENSPNAG00000036075.1"/>
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        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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            <begin position="12"/>
            <end position="191"/>
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            <position position="15"/>
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        <ligand>
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            <label>1</label>
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    </evidence>
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    </evidence>
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            <dbReference type="Pfam" id="PF03372"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="194" mass="22410" checksum="EEBDBD45D2628C97" modified="2024-11-27" version="1">EVGNPPHGQRIVTLNVNGLNSPIKRERVLRGLKREKAQIIFLQETHLSPAEYKNRRGVAIIFQNSLNFELTKEINDKEGRFVLLQGRLQQMPITLFNIYVPPNSNKAFFHIILDLLASKSKATLICGGDFNLVLSHRLDTTSIKRLQSRETRIMNNILVELGLLDVWRELHNGDRVYTYYSVPHKVHSRLDCER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K6P4</accession>
    <name>A0AAR2K6P4_PYGNA</name>
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        <recommendedName>
            <fullName evidence="4">REJ domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="primary">CARMIL1</name>
    </gene>
    <organism evidence="2 3">
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
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        </lineage>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
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                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2016" mass="202351" checksum="72040E08A3201795" modified="2024-11-27" version="1">MVVMGTRSLSTTHNYFIYLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSLCLSLCLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSLSLCLSLSVSLCLSLCLSLSLSLSLSVSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSVSLCLSLSVSLSLCLSLSVSLSLSLSLCLSLSLSLSLSVSLSLSLSLCLCLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSVSLSLSLSLSLSLSLSLSLSLSLSVCLSLCLSLSVSLSLSVSLSLCLSLSLSLSLSLCLSVSLSLSLSLSLCLSLSVSLCLSLCLSLSLSLSLSLSLSLSLSLSLCLSLSVSLSLSLSLSLSLSLSLSLSLSLCLCLCLCLCLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSLSVSLSLSLSLCLSLSLSLSLSLSVSLSLSLSLSLSLSLCLSLSLSLSLCLSLSVSLSLCLSLSVSLSLSLSLSLSLSLSLCLSLSVSLSLCLSLSVSLSLCLSLSLSLSLSLSLSLSLSLSLSLSLSLSVSVSLSLCLSLSLCLSLSLSLSLSLSLSLSLSLSVSLSVSLSLSLSVSLSLCLSLSLSLSLCLSLSLCLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSLSLSLSVSLSLSLSLSLCLSLSVSVSLSLSLSLSLSLSLSLCLSLSVSVSLSLSLSLSLSLSLSLSLSLSLSLCLSVSLSLSLSLSLSLSLSLSVSVSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLCLCLCLCLCLCLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSLSLSLSLSLSLSLSLSLCLSLSVSVSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSLSVSLSLSLSLSLSLSLSLSLSLSLSLSLCLCLSLSLSLSLSLSLSLSLSLSLSVSLSLSLCLSLSLSLSVSLSLSLSLSLSLSLSLCLCLSVSVSLCLSLSVSVSLSLSVSVSLSLCLCLSLSVSLSLSLSLSLSVSVSLSLSLSLSLSLSVSVSVSVSVSVSVSVSVSLSLSLSLSLSLSLSLSLCLSLSVSVSLSLSLSVSLSLSLSLSLSLSLSLSLSVSVSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSLSVSVSLSLSVSVSLSLSLCLCLSLSVSLSLSLSLSLSLSLSLSLSLSLSVSLSLSLSLCLSVSLSLSLSLSLSLSVSVSLCLCLSLSVSLSLSLSLSLSLSVSVSLSLSLSLSVSLSVSLSVSLSLCLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSVSLSLSLSLSLSVSLSVSVSLSLSVSVSLSLSLCLCLSLSVSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLCLSLSVSLSLSLSLCLSVSLSLSLSLSLSLSVSVSLCLCLSLSVSLSLSLSLSLSLSVSVSLSLSLSLSVSLSVSLSVSLSLCLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSVSLSLSLSLSLCLSLSLSLSLCLSLSVSLSLSLSLSVSLSLCLSLSVSLSLSLSLSLSLSVSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSVSLSLSVSLSVSLSLSVSLSLSLSLSLSLSLCLSLSVSLSLCLSLSVSLSLSLSVSLSLSLSLSVSLSVSLSVSLSLSLCLSLSLSVSLSLSLCLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSVCLSVCLSVCLSVCLSVCLSVCLSVCLSLSLSVCLSLSLSVCLSVSLSLSVCLSLSLCLSVCLSLSVCLSLSLCLSVSLSLSVCLSLSVCLSVSLCLSVCLSLSVSVSVSLSVSLSLSLSLSLSVSVSLCLSLSLSYVNTRCDSLLVKRTLGSVFVAICNISCGELWFPPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KL23</accession>
    <name>A0AAR2KL23_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Retinoic acid receptor RXR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nuclear receptor subfamily 2 group B member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for retinoic acid that acts as a transcription factor. Forms homo- or heterodimers with retinoic acid receptors (rars) and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate gene expression in various biological processes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Heterodimer; with a rar molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KL23"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066350.1">
        <property type="protein sequence ID" value="ENSPNAP00000064980.1"/>
        <property type="gene ID" value="ENSPNAG00000008197.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166019"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06956">
        <property type="entry name" value="NR_DBD_RXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06943">
        <property type="entry name" value="NR_LBD_RXR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000002">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000005">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021780">
        <property type="entry name" value="Nuc_recep-AF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000003">
        <property type="entry name" value="Retinoid-X_rcpt/HNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11825">
        <property type="entry name" value="Nuc_recep-AF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00545">
        <property type="entry name" value="RETINOIDXR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="142"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="237"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="469" mass="51735" checksum="42B458D8284A6890" modified="2024-11-27" version="1">MHYFTVKQLDLLFVVCPFFHLSVSGFLFSDSSGALNSSPSQPPPMGSMMGHPSVISSSRPLPSDMGPLGSPINGLASPYSVITSSMGSPSVSLPSTPGMGFSTLNSPQMNSLSSVSSSEDVKPPPGLPPLGYQCTSPGSMSKHICAICGDRSSGKHYGVYSCEGCKGFFKRTIRKDLTYTCRDSKECLIDKRQRNRCQYCRYQKCLAMGMKREAVQEERQRGREKSDNEVESTSTFNEEMPVEKILDAELAVEPKTETQTDGSPGNSTNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSVTVKDGILLATGLHVHRSSAHSAGVGSIFDRVLTELVSKMKDMQMDKTELGCLRAIVLFNPDAKGLSNPSEVERLREKVYASLESYTKQKYPDQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KLW4</accession>
    <name>A0AAR2KLW4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017562431.1">
        <property type="nucleotide sequence ID" value="XM_017706942.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KLW4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065048.1">
        <property type="protein sequence ID" value="ENSPNAP00000063101.1"/>
        <property type="gene ID" value="ENSPNAG00000034113.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108432834"/>
    <dbReference type="GeneTree" id="ENSGT00940000164930"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14721">
        <property type="entry name" value="bZIP_Fos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000006">
        <property type="entry name" value="fos-related antigen 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF52">
        <property type="entry name" value="PROTO-ONCOGENE C-FOS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="105"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="365" mass="39213" checksum="0F7E2A52AE04CCB7" modified="2024-11-27" version="1">MPRASSRTALCHQSKMKSAQGRGSDSGAADGPFVPTVTAIATAPDLKWMVLSTDISSVGPCSGAAKQKRAQAGQRKAQPSTRKAGHGAPAGRRKAQKEQLSPEEEERKRIRRERNKLAAAKCRNRRRELTDSLQAETDQLEEEKAALQAEISTLMKEKEQLELLLSTHNPHCKIPAEMPEEEEEEDEASEEPPKPSLVLQDDLNPIQSPSPTISLDKMPSSPLGLHQVPNCPQELEPTGPIMSEDSDVLLCSSAALEPYIDMKDVPMDDLGCKVDEDDNDDMDLLVPDIDLSASLGLTEWETLYMTMGGSLDPLSTPTLSPACGTSASSAVPMFDFPNLDSEGSDGKVGAAKAKAGADNSTLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KZI0</accession>
    <name>A0AAR2KZI0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Copper-transporting ATPase 2</fullName>
            <ecNumber evidence="4">7.2.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Copper pump 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">ATP7B</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Cu(+)(in) + ATP + H2O = Cu(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25792"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. Interacts with COMMD1/MURR1. Interacts with DCTN4, in a copper-dependent manner. Interacts with ATOX1. Interacts (via C-terminus) with ZBTB16/PLZF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017557714.2">
        <property type="nucleotide sequence ID" value="XM_017702225.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000062378.1">
        <property type="protein sequence ID" value="ENSPNAP00000069670.1"/>
        <property type="gene ID" value="ENSPNAG00000018792.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430056"/>
    <dbReference type="GeneTree" id="ENSGT00940000155749"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060003">
        <property type="term" value="P:copper ion export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd02094">
        <property type="entry name" value="P-type_ATPase_Cu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000015">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000092">
        <property type="entry name" value="copper-transporting ATPase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000144">
        <property type="entry name" value="copper-transporting ATPase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00003">
        <property type="entry name" value="copper ion binding protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF30">
        <property type="entry name" value="COPPER-TRANSPORTING ATPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00942">
        <property type="entry name" value="CUATPASEI"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0187">Copper transport</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="682"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="723"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="751"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="915"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="959"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1325"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1353"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="161"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="247"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="351"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="475"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="24">
        <location>
            <begin position="551"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="420"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1107"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1414"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="451"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1108"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1120"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1418"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="1437"/>
            <end position="1446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00065683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00074947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1468" mass="158334" checksum="F7574F9BEE339B97" modified="2024-11-27" version="1">MSRLDSRSSFGKRRRHDPGTEQVCMVDCGPLCLCRTEPCVCRSEGKENGRPHETWASKKHAFDNLAYEPSSPSELSLSRLTLLLSGSSSPESAQALHTWLSALSGVVAVSVPQAGGKAWIDYHPSTVTAREILQEIQGMGYGVSEVTNALSHPAPPRLEESLVRIRVEGMTCHSCARSIEGRIGALKGVLGIQVSLSEREAVIWFNPLDVKPEELREHVEDMGFEAFLKDPVLLQPSQAESLPTEGLEVRLGVEGMHCGSCVKNITESLSGMLGVQAVLVSLETKTVGLKYDPSLVTLDTVKGVLEGLPPLNFRVTLPGQSPQLSFHEPKTNGSIHSQKSISHSMDSIPLQKVTVGIKGMTCHSCVQSVEGLISQKDGVHSITVYLKEEKGIITFDPNLTRPAELRDAIEDMGFEAYLQQDSGQSSHVRGQGSHRSMSSHPKNLTPPAVRPQTSKSQAASVNSAKTGVPEEEKTRKCFVHVTGMTCASCVANIERSLLKHKGIKSVLVALMAGKAEIKYDPDDADPAQIIKLISSLGFGASLMEESAVSDGVLDLSVTGMTCASCVHNIENKLLRTKGILEATVALATSKAHVKFDPDLVGARDIIRIVEGLGFGVSLIKNDGLRKNEGLDHQEEIRQWKHSFLFSLVFGVPVMGLMIYMMVMDSQHSEHGGSMPEEQNVLPGLSILNLAFFLLCTPVQIFGGRYFYIQAYRSLRHGVANMDVLIVLATTISYVYSCIVLLVAMIEGAKQSPVTFFDTPPMLFVFIALGRWLEHIAKDKTSEALAKLMSLQATDATIVTLGHGNSIISEEQVSVELVQRGDVVKVVPGGKFPVDGKVIEGSSMADESLITGEPMPVRKKPGSCVIAGSINAHGALLVEATHVGADTTLCQIVRLVEEAQTSKAPIQQLADKLSGYFVPFIVIVSVVTLAVWLVIGFLDFDIVTKYFPGYDKNIPRTDVIVRFAFQASITVLSIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKIRAVMFDKTGTITNGVPRVTRVLVLWDQARLPLRKVLAVVGTAEASSEHPLGMAVAKHCKEELDTEMLGYCHDFQAVPGCGISCKVSNIEELLQNSPETESTHTSPTQLTLQGATTDESSLVTDSDPGPTTYSVLIGNRQWMQRNSVQVTPDVDEAMSSHETKGQTAILVAIDGVLCAMLAIADTVKEESALAVHTLTRMGIDVLMITGDNRRTAKAIATQVGIRKVFAEVLPSHKVAKVQELQEKGLKVAMVGDGVNDSPALARADLGIAIGTGTDVAIEAADVVLIRNDLLDVVASIELSKKTVQRIRINFVFALIYNLLGIPIAAGVFMPAGLVLQPWMGSAAMAASSVSVLVSSLLLRLYKKTTVEQYESRAHGRMSSLSSSQVSTHVGLEERRCSPRALQRGWSRHSQSSHSSGMPSQPPTLSGRSKTQPYPHLDRHSLLEYDQHGQELVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L5K3</accession>
    <name>A0AAR2L5K3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L5K3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084457.1">
        <property type="protein sequence ID" value="ENSPNAP00000069974.1"/>
        <property type="gene ID" value="ENSPNAG00000037174.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000277415"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="147"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="292" mass="31673" checksum="ABF2F067100C2CBA" modified="2024-11-27" version="1">MATAGFFSCSECGMFSLDPFSTDSHNDSGVCSKCKLVNCLVEKVDKLELRIRGLIRDRQQGSLLAAPGTPGRASTPSTLALEPPQQGEWVTSRRHTSPPVHHAPPVHVSNRFAPLSEAPAEEPVKGTLVIGDSIVRHVKLATPLGAPAARVICLPGARAPDISGNLRLLANRRYSRVVIHVGANDIRLRQSEVTKANINEVVKQAQTLSEEVICSGPIPMRRGDEAYSRLSSLNRWMSKWCTDNHVGFIDNWLMFEGKPGLLGRDGVHPTRDGAALLSCSIAHSLLVNPMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L9C1</accession>
    <name>A0AAR2L9C1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">XPOT</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <text evidence="10">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571197.1">
        <property type="nucleotide sequence ID" value="XM_017715708.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L9C1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069554.1">
        <property type="protein sequence ID" value="ENSPNAP00000073183.1"/>
        <property type="gene ID" value="ENSPNAG00000009140.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438122"/>
    <dbReference type="CTD" id="11260"/>
    <dbReference type="GeneTree" id="ENSGT00390000007890"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000105">
        <property type="entry name" value="Exportin for tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="11">
        <location>
            <begin position="109"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="12">
        <location>
            <begin position="329"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="972" mass="110660" checksum="E91E1434F213C157" modified="2024-11-27" version="1">MACESALPAIMDEQALMGLNPNADACYRQRALAYFEQLKESQDGWEVCGEALAKGIYSDDHVKFFCFQVLEHQIKYRHGSLTGAQQQLIRETLMKWLQLQLMSTQSEKPFIKNKAAQVFALTFIMEYLTVWPKFFFDLLSLVGLSPHGLDIYLRTLMAIDAEVVDRDILHSPEETRRNTLIKDSMRENCIPALVESWYQILQTYQQSNSELTCQCLEVVGAYVSWIDLNLIANDRFVNLLLSHMSVEELREEACDCLFEIVNKGMDPVDKTKLVESLSQVLQSAGFFIVEQEEDVDFLAKFSRLVNGMGQALVLSWTKLSKMGDVKVAAETLRALEAKVPLMLQLLIHEDDDISANIVGFCYDYLHILKQLPLLTDQQKNNVEAVMLAVMKKLTYDDEYNFENEGEDEAMFVEYRKQLKMLLDRLAQVSPELLLESVRRVFTSTMQNWQTARFMEVEVAIRLFYMMGEALPASHGAHFTGDATKSSALQDMMRTLISSGVSEYHHTSVTLEFFETVVRYDKFFIVEPQHIPNVLMAFLDHRGLRHSSPKVRSRVAYLFSRFIKTLHKHMNAYIEDILSRIQDLLELAPPENGFLALLSNDDQLFMFETAGVLIVNGECPADRKQALMRSLLTPLMEAFRLLLAKLTQEADEERQTALADCLSHAVGFASRTSKAFSNKQTVKLCGCSEVYLDCLQTFLPALSCPVQRGTLRSAVRSFLHRMIICLEEEVLPFIPAASEHMLKDCEPKDLQEFIPLINQITAKFKSQVSPFLQQVFMPLVLAIFEVLSRPAEDNDQTAALENQMLRRSYFSFIQTVASSGMNEVMANQGAENIERVLFTIIQGAVDFPDPVAQKTCFIILSKLVELWGGKDGLVGFPDFIYKHIVPACFLAPLKPTFDLSDAQTVLTLSECTLTLKIIHLKRGLEFVQFLQQEYLPSLQVSPEITQELCQVLQQPDTKVLKNYMKAFFQRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L9M9</accession>
    <name>A0AAR2L9M9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transferrin receptor protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TFR2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037397214.1">
        <property type="nucleotide sequence ID" value="XM_037541317.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397215.1">
        <property type="nucleotide sequence ID" value="XM_037541318.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397216.1">
        <property type="nucleotide sequence ID" value="XM_037541319.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397218.1">
        <property type="nucleotide sequence ID" value="XM_037541321.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397219.1">
        <property type="nucleotide sequence ID" value="XM_037541322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L9M9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051185.1">
        <property type="protein sequence ID" value="ENSPNAP00000073298.1"/>
        <property type="gene ID" value="ENSPNAG00000033557.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108412633"/>
    <dbReference type="GeneTree" id="ENSGT01030000234598"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004998">
        <property type="term" value="F:transferrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140298">
        <property type="term" value="P:endocytic iron import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033572">
        <property type="term" value="P:transferrin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09848">
        <property type="entry name" value="M28_TfR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02128">
        <property type="entry name" value="PA_TfR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000002">
        <property type="entry name" value="Transferrin receptor protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037324">
        <property type="entry name" value="TfR1/2_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF33">
        <property type="entry name" value="TRANSFERRIN RECEPTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="13">
        <location>
            <begin position="230"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="14">
        <location>
            <begin position="407"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="786" mass="86062" checksum="C5082A1945F69F06" modified="2024-11-27" version="1">MADPVRRLLTGSPGGGAQADEGVEEGGGTGERKTRVEMKLIQPRSEDSSGAGLTNDITAMMLQQQVNRRKAVLYLTLTGLLIFTTAFLLGYVSFRGMCRLCVDEGEMVPVEDPAGPDQPAGGGVMYLGELRDMLKKYLNEEKIESTVRRVSRATHSTGSAEGNALASEILQNFQKLHMDHTWTDSHYATLQFPSRTQRNALWLVDAEGVELEEIPLDSADYCAYSAMGTVTGGVVYVNYARQEDFEQLRALGVALNGSIAMARVGGGVSFAEKVWHAQEAGLVGILFYPDPADIPQDPRRLGLHGNAAISEHVHLGSGDPFTPGFPSFNHTQFPPTQSSGLPLILAQPVSANVASKLLSQLVGVACPRGWQGRLPYVRCVVGPEFSVGGRRVRMAVYNSMSPVLLNNIFASIEGKTEPDQYIIMGAQRDAWGPGAVKAGVGTAVLLELARTFGTMLQNGFYPRRSLLFVSWDAGEFGNVGATEWLEGYLSMLHLKAVAYFSLDQAVMGDDALSAYTSPLLADLIEGAIKQVDHPKHSGQSILSLAEAQGGWRSKIVKPLYLNSGAYSFTAFGGVPAMELRFHEEGRVYPFVNTQLDSAERVQELLQGRLGVVGRTVGELVGLMVLKLTHDLILPLDVTCYSTAALRLSAQLNQLSAELQVRGLSPQWVYRARGDYSRAAKTLQEAIKNTDIQDERMARLYNTRIMRVEYYFLSQYVSVVETPFRHVLHGRGDHTLSALTEHLALLRTQPQLFNEARFRQQLALFTWTLQGAANALSGDVWNIDNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBY8</accession>
    <name>A0AAR2LBY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Voltage-dependent calcium channel gamma-4 subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574029.1">
        <property type="nucleotide sequence ID" value="XM_017718540.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBY8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066043.1">
        <property type="protein sequence ID" value="ENSPNAP00000074035.1"/>
        <property type="gene ID" value="ENSPNAG00000030293.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108439892"/>
    <dbReference type="CTD" id="550428"/>
    <dbReference type="GeneTree" id="ENSGT01050000244893"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016247">
        <property type="term" value="F:channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099590">
        <property type="term" value="P:neurotransmitter receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098943">
        <property type="term" value="P:neurotransmitter receptor transport, postsynaptic endosome to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051968">
        <property type="term" value="P:positive regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000004">
        <property type="entry name" value="Voltage-dependent calcium channel gamma-4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051072">
        <property type="entry name" value="CACNG_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008368">
        <property type="entry name" value="VDCC_gsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107:SF2">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-8 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01792">
        <property type="entry name" value="VDCCGAMMA"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 15" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="6 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="10 15" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="252"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="374"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="45951" checksum="790F50B328617AB1" modified="2024-11-27" version="1">MDGKGRHIPPAMVWCERGIQVLLTTVGAFAAFALMTVAIGTDYWLYARAFICNSTANSTQDDPHNKDKKDPGALTHSGLWRICCLEGLKRGVCSQINHFPEDADYDQDAAEYLLRVVRASSIFPILSAILLLLGGVCVASSRFYKNKRHIILGAGILFVVAGLSNIIGVIVYISAALSDISPKKDEDKKWHYSYGWSFYFGGLSFILAEVVGVLAVNIYIERNKELRCRSRTDLFRSTTSAVLRLPSYRFRRRSSRSSSRSTAEPPRSRDQSPSGLAAKNFGPPLAAGPPPFSVATLPNPHTHSGGGGGGGGIGDISMYTLGREGKPPMYGTVDRATLYQLHNYFPTKEGGGGALMTGTLPSLSKSNLAASANAPLNASSSSGPGVQAPTLSAGSTATMERERGLGTLDRLGKGDSSNTNTLNRRTTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LEL8</accession>
    <name>A0AAR2LEL8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LEL8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053689.1">
        <property type="protein sequence ID" value="ENSPNAP00000075038.1"/>
        <property type="gene ID" value="ENSPNAG00000032394.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178790"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029676">
        <property type="entry name" value="CFAP221"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46500">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46500:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="113" mass="13042" checksum="CCAB3BC8543FEFA7" modified="2024-11-27" version="1">MEVMLQAPKNFTSNVQKKKILPLCQLVEETRRTDNVTHHLLETRVYAKLKSNNVVEVAPLELHFSGFEVGKDYKKVVVCKMFLGIYSEIVQCHGKMLAPFLIYCIPIEDFRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LFY7</accession>
    <name>A0AAR2LFY7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TST</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LFY7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070845.1">
        <property type="protein sequence ID" value="ENSPNAP00000075508.1"/>
        <property type="gene ID" value="ENSPNAG00000036478.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000046773"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000001">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000008">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008557">
        <property type="entry name" value="PRK11493.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="23"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="172"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="295" mass="32871" checksum="67EF738F06881304" modified="2024-11-27" version="1">MAGQVKALVAAKWLAEAVKNNQVGPSLRILDASWYLPKLNRNAQEEFRQRHIPGASFFDIDDCCDKSSPFDHMLPAASEFADYVGDLGIGNQTHVVVYDGSDFGSFSAPRVWWMFRLFGHNSVSVLNGGLKTWLREGHPTADRYTKPERSEFKAQINSSWVKTYEDVLENLASKKVQLVDARVEGRFRGLEPEPREDTEPGHIPGAINMPFPSFMDPSGLELPVEELTKLFEQAGVHLQKPFWVSCGSGVTACHITLAAYLCGHQDVCLYDGSWSEWFAKAAPEHVISEGKGKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIC5</accession>
    <name>A0AAR2LIC5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Peptidase M12B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ADAMTS2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575771.2">
        <property type="nucleotide sequence ID" value="XM_017720282.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000044052.1">
        <property type="protein sequence ID" value="ENSPNAP00000074407.1"/>
        <property type="gene ID" value="ENSPNAG00000004618.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441024"/>
    <dbReference type="GeneTree" id="ENSGT00940000156647"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF141">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9 15 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043456684" description="Peptidase M12B domain-containing protein" evidence="18">
        <location>
            <begin position="22"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="19">
        <location>
            <begin position="253"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="20">
        <location>
            <begin position="1040"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="active site" evidence="14 17">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="330"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="373"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="412"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="499"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="526"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="560"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="564"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1333" mass="149838" checksum="C16357721DFC3DA9" modified="2024-11-27" version="1">MAVLPGYAFFILLLHFQLARALYIADSADSLQHVLREYGLVKPVSVDEDGRFLSHSVSVGQLSRQQPLRRKRRDTHLNGVQEHASTRETLFYNVTVFGQEFHLHLRRNSRLVAPGAMVEWLEEDNQTHSEPLLASDCLYVGHVTNVPDTSVAISNCDGLAGMIRAGQEEFFIEPLERGGGDMTGEEEGGDGRHHILYRSSAIKKPAISHTDDFPPRVSALGGLAGLEALSRGFEQKVNSSRRSKRQSHQDEIFNIEVLLGVDCSVVQFHGREHIQKYLLTLMNIVNEIYQDGSLGAHINVVLVRIMMLCSPKSIGLIELGNPSQSLENVCRWAFLQQKEDENDAEYHDHAIFLTRREFGPTGMQGYAPVTGMCHPVRSCTLNHEDGFSSAFVVAHETGHVLGMEHDGQGNECGDEVPMGSIMAPLVQAAFHRFHWSRCSHQELSRYLNTYDCLRDDPFDHEWPSLPQLPGLHYSMNEQCRFDFGMGYMMCTAYSTYDPCKQLWCSHPENPFFCKTKKGPPIDGTKCATGKHCFKGHCIKLSPDILRQDGHWGQWTDFGSCSRTCGGGVRFRTRQCDNPIPANGGRTCYGNNYEFQLCNTDECAKAQADFREEQCKMWDPHFEHQGAKHHWLPYEHPEPEERCQLHCQSKETGDVVSMKRMVHDGTRCSYKDPHSVCVRGECEKVGCDNVIASELEDDKCGVCGGDNSTCKIVKGNFTRSAKKLGFLKILEIPRGARHVFIREFKGTPHILAVKNQATDHLFLNDEGEFPESRTVIEKGVLWEYSNDDDMETVQTTGPLGYGVLIMVQSHSTSKVTLSYKYILPEDPASSMENSLPQDDTVYFEWALKKWSHCSKPCGGGKQYTRFGCRRKSDGKMVHRTLCANIAKPRAISRACNQKQCSEPVWVTGEWEECSKSCGKTGTQTRPVRCVRPQPDGTQKTINSKYCNDDRPEGRRSCNRQLCPAQWRTGPWSQCSVTCGNGTQERQVMCSTPDNAIGVCLDTKPENIRKCQLMPCSGDKGNFLIQWLSRADPEFPAPKISSRTRCKGDKSVICRMEVLSMYCSNSGYRQMCCKSCSEGNNTSSLNLTSKPWTYISVAPGTIIPMVRNVTQPYTLRQVPITHSYYTTIKSSLSKQGSPTSTPTPWRYTTTLNPLTLRRGTTFPITTSAFLEETDGPSTAFDEEDSSESWTVITSNEVSGSTTWFEDFSSTVVQSSTTPRYVGAEKLVTTQTVTMPPVTTPTVTTPAVTTAVQSRFIITSPPAIVSLHRSEDRKENNSIDVSYKVVDVNEVAQNHFISRKRVLFQEKTHNKRIQELLAEKMRQDFLLRRLKRKPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIJ6</accession>
    <name>A0AAR2LIJ6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">C15orf61</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LIJ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061851.1">
        <property type="protein sequence ID" value="ENSPNAP00000076378.1"/>
        <property type="gene ID" value="ENSPNAG00000030523.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015942"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029245">
        <property type="entry name" value="DUF4528"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34651">
        <property type="entry name" value="SIMILAR TO ENSANGP00000021391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34651:SF1">
        <property type="entry name" value="SIMILAR TO ENSANGP00000021391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15031">
        <property type="entry name" value="DUF4528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="156" mass="17795" checksum="8DC53BCE01F2006C" modified="2024-11-27" version="1">MKEILRKIHSGFLNLLLFPSAFRNKRPRPPASEVLTCHLSQRRLPPWTSFCVRYSAVENDQFGLSSFNWQVQDANYHILRTGAFPFIKYHCTRAPPQDLDFENTFFGALKVINLGVPCLAYGLGSWMVVRVTETVQTSAGPVTVYFAYKEVEGAQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LMS9</accession>
    <name>A0AAR2LMS9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017580791.1">
        <property type="nucleotide sequence ID" value="XM_017725302.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017580799.1">
        <property type="nucleotide sequence ID" value="XM_017725310.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017580809.1">
        <property type="nucleotide sequence ID" value="XM_017725320.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LMS9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081004.1">
        <property type="protein sequence ID" value="ENSPNAP00000077870.1"/>
        <property type="gene ID" value="ENSPNAG00000027410.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444250"/>
    <dbReference type="CTD" id="220972"/>
    <dbReference type="GeneTree" id="ENSGT00940000158282"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16807">
        <property type="entry name" value="RING_CH-C4HC3_MARCH8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000043">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase march8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45981">
        <property type="entry name" value="LD02310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="23">
        <location>
            <begin position="325"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="333"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="542"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="561" mass="61632" checksum="B4BF742EAF79857F" modified="2024-11-27" version="1">MNMPLHQISVIPRDVTSSRGSGSSGKAKDKDRDKQNDKPLGHSASRSSNISKAGSPTSVNAPCSFSRTSVTPSNQDICSSNGGSRVQAAQTAVVLLNSDGKGLVSQSVTMTTLCLDSPAKPKNKTAFGAEWLSLLKPSVGLKPNSGALKFSKSLNDVGQKMDSLCSGLHFIDRTCSEGELGPETEPKALNGKPAHLPSPPLPSQPLSFSSTCAYGSSLGQERCSQTSELQKSSNLLRFLFPFPRSSTAGSLQAGELTSYITKSSSALLEACGEEMGDDEVFEDDPSQWRPLKAEERRAPLCSLEEDSDLDRCSSPLSEKAAPLSPYSLSGDCCRICHCEGDDESPLIMPCHCTGSLRFVHQACLQQWIKSSDTRCCELCKFEFIMETKLKPLRKWEKLQMTASERRKIMCSVTFHVIAITCVVWSLYVLIDRTAEEIKQAGRIPGALKLHPGLTYGASKSRILEWPFWTKLVVVAIGFTGGLVFMYVQCKVYIQLWRRLKAYNRVIYVQNRPETCKKIVFEKPAFMEPNLDNKEALGLAQSDTNSSQYTETEDYSMEILHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LSZ5</accession>
    <name>A0AAR2LSZ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Platelet glycoprotein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Glycoprotein IIIb</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">PAS IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">PAS-4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Platelet glycoprotein IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">CD36</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(9Z)-octadecenoate(out) = (9Z)-octadecenoate(in)</text>
            <dbReference type="Rhea" id="RHEA:33655"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:33656"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(9Z,12Z)-octadecadienoate(out) = (9Z,12Z)-octadecadienoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45264"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:45265"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>butanoate(out) = butanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45248"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:45249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>hexadecanoate(out) = hexadecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45256"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:45257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>tetracosanoate(out) = tetracosanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45260"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:45261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>tetradecanoate(out) = tetradecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45252"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:45253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane raft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LSZ5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076115.1">
        <property type="protein sequence ID" value="ENSPNAP00000077854.1"/>
        <property type="gene ID" value="ENSPNAG00000009647.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153372"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030169">
        <property type="term" value="F:low-density lipoprotein particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150094">
        <property type="term" value="P:amyloid-beta clearance by cellular catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034383">
        <property type="term" value="P:low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005428">
        <property type="entry name" value="CD36/SCARB1/SNMP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF12">
        <property type="entry name" value="PLATELET GLYCOPROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01610">
        <property type="entry name" value="CD36ANTIGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23 32" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="25" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="26" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="21 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0564">Palmitate</keyword>
    <keyword evidence="24" id="KW-0675">Receptor</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 33" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="32">
        <location>
            <begin position="235"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="32">
        <location>
            <begin position="264"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="32">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00000996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00020772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00029966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00031821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00032188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00032780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PIRSR" id="PIRSR605428-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="471" mass="52818" checksum="705837A43814AC6D" modified="2024-11-27" version="1">MGCSCCSTRCWLITGSVAGAVACLLGVILIPVGNTVIEDTVKKEAVLEKGTTAYENWISAGAPVYRQFWLFDVQNPDEVVANGSIPQLQQRGPYTYRTRYIPKQNITFSENHTVSFVLPAEAVFEPSLSHGFEEDKVTSLNLAVAGVYNLIDHAWANMFIESSKSSLFLRRTVRELLWGYTDPMLKRTLGVFYPYNNTYDGPYGVFTGKDDINKVSMIDTWNNQPTVPYWNNSYCNMINGTDGSSFPPFLDKKKALYFFSSDICRSVSADYDGSVDLKGITVYRYMLPEQTFASPTVNPDNKCFCTNYEATKNCTLAGVLDIKSCKGFPVFISLPHFLHGSPELLDTVHGLNPNPEEHSTYLDVEPITGFTLRFAKRLQINMMYGPSKEIKILNKVKENTVFPIVWLNESAALDDETAARIKNDLFARIETLEVVQISLIVIGAVVFVLCLIGICIQERRRRKIIANIHDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LXE9</accession>
    <name>A0AAR2LXE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch domain containing 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KLHDC2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017569307.1">
        <property type="nucleotide sequence ID" value="XM_017713818.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LXE9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077975.1">
        <property type="protein sequence ID" value="ENSPNAP00000081263.1"/>
        <property type="gene ID" value="ENSPNAG00000030891.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108437007"/>
    <dbReference type="CTD" id="23588"/>
    <dbReference type="GeneTree" id="ENSGT00940000157150"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228:SF3">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="49974" checksum="E6A53946E4539F7D" modified="2024-11-27" version="1">MAEMDDDGDLHDVRDEDDDDSDQEEEGDFEWDAPEDARDFDWVEEQEVVLDADTSKSFHLDTPAERSGHIAVVDRNCMYVWGGYKNAEATAFFDLYLPKNEVWIYNMEMGRWRMKLSKGDLPAPMSGSCGVCVDGILYLFGGHHARGNTNLVYRLPLRASTLFWERMKDLKGLAPTCKDKLGCWVYKNKLAYFGGYGYVAQAGHRGTFELDENSLMGNHVGRGWNNHIHVLNLETLSWSQPITKGNAPTPRAAHACSTLGNRGYVFGGRYMDHRLNDLYYINMDTWEWSEMSVPQHGPVGRSWHSFIPVSSDHIFLFGGFTTNRETLSDAWLYCVSTNEWRPFKHSHTESPRLWHTACSGPDGEVFVFGGCANNLLSHQRAAHSNELLIFNVQPKSLLRSCMEVIVHHRQQLESMWDCLPKHLLHSIKQWIGTTNTGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZU4</accession>
    <name>A0AAR2LZU4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LZU4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073279.1">
        <property type="protein sequence ID" value="ENSPNAP00000082088.1"/>
        <property type="gene ID" value="ENSPNAG00000000503.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156225"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23612">
        <property type="entry name" value="TFP_LU_ECD_BMPR1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000001">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000055">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF50">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN RECEPTOR TYPE-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20" id="KW-0464">Manganese</keyword>
    <keyword evidence="17 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="25">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="265"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="563" mass="63896" checksum="B93CC8EF5330CCFC" modified="2024-11-27" version="1">MLLTSQLETFVLFEGLLYKNLLVAFNYTCFLRALCCTFICVTVMLLQVRITVVSVVTGQNPDHVLQGTGAKHSPDSRRPGADTTVEPEDATRFLSCYCSGHCPEDAKNNTCLTNGQCFAIIEEDENGDVFLSSGCMKYEGSHFQCKDSQYAQTRRTIECCQSDFCNRDLKPELPPRNPETFFSPHWLAFLISVTVCCCTLVCVTVICYYRYKWQTERQHYHRDLEQNEDLIPAGESLKDLINQSQTSGSGSGLPLLVQRTIAKQIQTVRLIGKGRYGEVWLGRWRGEKVAVKVFFTREEASWFRETEIYQTVLMRHENILGFIAADINGTGASTQLYLITDYHENGSLYDYLKFTTLDTQALLKLAYSAACGLCHLHTEIYGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVKFNSDTNEVDIPLSTRMGTRRYMAPEVLDETLNKNHFQAYIMADIYSYGLVIWEMARRCITGGIVEDYQLPYYEMVPPDPSYEDMLQVVCIKGLRPTVSNRWNSDECLRAMLKLMSECWAHNPASRLTILRVKKTLAKMVESQDIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M0A7</accession>
    <name>A0AAR2M0A7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M0A7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071005.1">
        <property type="protein sequence ID" value="ENSPNAP00000080352.1"/>
        <property type="gene ID" value="ENSPNAG00000037457.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000290152"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12660" checksum="3A538D3FDB3F731D" modified="2024-11-27" version="1">FAYYWSQSDLVKFQGQDFQTLRQSYISKQEKFIDEKFPPEMRSIGTGLLSQKETVKVVWKRPSVRQRPALLSFLLMLTETFIFMLGFESYCVMICLIPTSVLSKVSGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M4B6</accession>
    <name>A0AAR2M4B6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M4B6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078657.1">
        <property type="protein sequence ID" value="ENSPNAP00000082044.1"/>
        <property type="gene ID" value="ENSPNAG00000030297.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271879"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="391"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="861" mass="99189" checksum="8D480C212D9FF533" modified="2024-11-27" version="1">MDIECIGLNVSLSPEMSFIVICLYRPPSSNSLFYEHLYSILKECDEKKELILMGDFNINWENKQDRKRLKEITDKFSLTQLVQGPTRVTKCSQTQIDLMFTNRQERITKSSNLLTGLSDHNFILVVRKLTKKRFKNIIKPKHYYQKIPKSEMHNLENALKQIDWLNILSCKDIDDNCDIFLSTINKVMSSFTRKVQSKPRQRETLPWLNDALLQQMKERDFALKRTLKTGLNNDRLIFTSLRNKVVRNIRKAKAEFFIKIIDNARGNGKMIWQNLNKLTGRNNDQRTKEYELKVNGTLIQDHDMIANTFNNFFINSIEQLTQSFSSKTIISTIIDDDIPIFRIMETTESAVLNIINSLRDSKAKDVYGLDTTFLKLHKGILASPIAKLINHSIRQGIFPSAWKSAVITPIFKAGDRTVVENYRPISILPAVSKVAEKWVVQQLTAHLNKSYTPLHSMQFGFRSNHSTETAICYFLEGVKSKLDKGGVIGAVFLDLKRAFDTVNHKVLLSKLSCFNFSIDSVKWMNSYISNRKQRVRIGKTQSTYLSCNTGVPQGSVLGPILFSLYINDLPSVCSSVNIQMYADDTVLYVHAKNKQQAASELTAAMVLISDWLSNSCLYLNINKTVCMFFSQKLTEMIQADVLVRGEKLKLVSDTKYLGIILDSNLSFKNQVKKVINTVKLNLANFRHIRPYLTTEAAKLFMHAMIVSHITYCFTSWSQTSLSILKPIESLYKQALKILDRKPNSYHHCHIVQKYNLLSFDSFKHLLDACLMFKILYGLAPPPLRQFIKQKDSRGSMVTRAFTRGDCIVQYRRSKFGQTVFSIRASQYWNSLPTELRTCTNYLIFKYKLKTWLKANQICNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M4H0</accession>
    <name>A0AAR2M4H0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PTPRH</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M4H0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061535.1">
        <property type="protein sequence ID" value="ENSPNAP00000082012.1"/>
        <property type="gene ID" value="ENSPNAG00000021495.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155603"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF2">
        <property type="entry name" value="INTERLEUKIN-12 RECEPTOR SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043871178" description="Fibronectin type-III domain-containing protein" evidence="12">
        <location>
            <begin position="32"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="602"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="228"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="506"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="696"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="696"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="722"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="753"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="778"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="812" mass="91757" checksum="D0072C6B3FFA27C7" modified="2024-11-27" version="1">MGSLTGLIMQTTNLFMVIILQALCKASGVSGLEKCDVFPSNQLIKYGLDLRVSFRAPPKSDCRKITSFNTNRLYWTLNNQTIAKSFYHSNSTFASVFIQDFTLAVGIVECHLDDIVLGGTVIRTYLDPENVSCIAETLHNCFLCIRLTCKWDHNQNGVNTNYMVHVKQKGNESLCQSREIKWCTFSEEEGPKVGGEINVTVTAQTSAGEAHSEKVTYDSIWDIVKLDKPRDVQVKPELSALSVTWRSNNWALAYTVCEVRFLEKGSEPVVRTVEKTSSSTFDLTEVKPCTSYSVSVRCRIDVWSEWSQAVTHFTSINVSNVQLHLWRSEGVVYAKGKRAMHLMWKGINPLCKVFDEYRLFNRTHNLVTSKSFKPSENHTVVILDENAHSVTVAAYRNNQSLSEASMEIPSAAEEFNLPPVDNVNMFARDGQIHITWDKLYLTVTGYIVIWNSTEEDHQWQQTQERHFSLEGENFTLYTVSLMPLYENGPGSEIRLHAYAREGVPAVVSNVEAERICDRKAEIHWRPILSTQCCAFVVSYTVFYKAQNDMAFRNVTVDQKENGVILDDLQPKTNYIVYVQASSMAASSNSSFYTFSTKPYGEVFLIMLIVCGVGLMLLPVCTVLVVLIRKEYLNEKVPNPRFSSLTIWSSQKCRNPWNQLPVPWACDSEKSLTWQVEAEDYSILATSIRDVAKLQQNTATQETTPTTHTENTALVSSSSETELASRGKEERIKPLSEMDLETAQIHPVFLSRSPYKNQTPLTSPVDSPRKGLQGTTKPRSSEETETLLKPKHDSATMVTTYVTVDMFEHGKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BY10</accession>
    <name>A0A3B4BY10_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-beta hydroxysteroid dehydrogenase/isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560048.1">
        <property type="nucleotide sequence ID" value="XM_017704559.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BY10"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004473"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006943.2">
        <property type="protein sequence ID" value="ENSPNAP00000004473.2"/>
        <property type="gene ID" value="ENSPNAG00000011027.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431428"/>
    <dbReference type="CTD" id="3283"/>
    <dbReference type="GeneTree" id="ENSGT00940000155444"/>
    <dbReference type="OMA" id="GGKFYFV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000220">
        <property type="entry name" value="3 beta-hydroxysteroid dehydrogenase/Delta 5--&gt;4-isomerase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="11">
        <location>
            <begin position="11"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004473.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="376" mass="42052" checksum="0E83A6638D100A18" modified="2024-11-27" version="2">MAMSLSGDICVVTGACGFLGEKLVRLLLEDEKLLEIRLLDRNIGPGFIQSLEGCRGETKLTEFGGDIRDRELLKRACRGATVIFHTASLIDVIGAINYSELHGVNVKGTQLLLETCIQENVPSFIYTSSIEVAGPNPRGEPIINGNEDTAYTLCLKFPYSKTKKEAEQLCLSAQGELLENGAQLATCVLRPMYIYGEGCRFTLGHMRDGIRNGDVLLRTSQREAKVNPVYVGNVALAHLQAARALKDPQKRAVVGGNFYYISDNTPHVSYSDFNYAVLSPLGFGLQDKPTLPFPVLYLISFFMEMLQAVLRPFLRFTPPLNRQLLIMLNTPFSFSYQKAHRDLGYTPRYEWAEARQRTTDWLASVLARERERISAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0Z6</accession>
    <name>A0A3B4C0Z6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a transporter for creatine and as well for its precursor guanidinoacetate. Transport of creatine and GAA is independent of resting membrane potential and extracellular Na(+), Cl(-), or pH. Contributes to the process of creatine biosynthesis and distribution.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>creatine(in) = creatine(out)</text>
            <dbReference type="Rhea" id="RHEA:73043"/>
            <dbReference type="ChEBI" id="CHEBI:57947"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanidinoacetate(in) = guanidinoacetate(out)</text>
            <dbReference type="Rhea" id="RHEA:73047"/>
            <dbReference type="ChEBI" id="CHEBI:57742"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0Z6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004501"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006911.2">
        <property type="protein sequence ID" value="ENSPNAP00000004501.2"/>
        <property type="gene ID" value="ENSPNAG00000001473.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156169"/>
    <dbReference type="OMA" id="DTIGRFN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015881">
        <property type="term" value="P:creatine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000128">
        <property type="entry name" value="monocarboxylate transporter 12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF318">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="15"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004501.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="443" mass="47661" checksum="412770E2D26358C0" modified="2024-11-27" version="2">MAEGGRRPAAPDGGWGWMIVAGCFLTTICTRAVTRCVSIFFVEFHMHFSSDYSRTAWIHSLVDCTTMLCAPLGSYIGNHLSCRAAVMVGGLLSSVGLILSSFATSLEYLYLSLGVLTGVGFAFCYTPVVAMVGTYFKARKALAYGIAMSGTGIGTFILAPVVQLLIELYSWRGALLILGGLVSHLCVCGALMRPLLAKAVFVDSELAESIEANSVLTGPGREKEGHFFPPSTDECRFLLMPDFLLLSVSFLFLAFGCSVPFVYLVPYSLSVGISHQQAALLMSVLGVMGIVGNITFGWISDRKCLRTYRIVSYLLAIVLEGLACLFVPLLRNFSLLVAFALFYGYFDGAYVALLPVVTSDTVGPTYLSSALGVVFFLHAIPYLISPPIAGWLVDQTGSYTAAFLLSGFSLICSASILATVSLARRCNRSISEPKLMLILPAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C141</accession>
    <name>A0A3B4C141_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAM186A/B C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C141"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005483"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005223.2">
        <property type="protein sequence ID" value="ENSPNAP00000005483.1"/>
        <property type="gene ID" value="ENSPNAG00000011688.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000205462"/>
    <dbReference type="OMA" id="EMNINHS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049146">
        <property type="entry name" value="FAM186A_B_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20865">
        <property type="entry name" value="FAM186A-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM186A/B C-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="254" mass="29684" checksum="F5D623E5D1DF710C" modified="2018-12-05" version="1">MPHRYQMNKETQRTNLLLLQNALRKGQISLQLYKQARALLENIPSVKLKCLAFLLRKYRAFKALQRVRYQLNIKIRFAREHKDGQALKEYYIILSKLDKYEKEVLGRWRRRQEAQEQKYHVNLTRIIYMFHQLREAYSLNLLNPNSSQSYSIGKHFLAFKPQGRQRSVISREHCSPFPSVKTPTYLLRPRNITTSHPGAREPVGSEAPTVWKCSQTLDDVQLTSTVPIIPRLLEMNINHSTISALRTVQGRTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C142</accession>
    <name>A0A3B4C142_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017541658.2">
        <property type="nucleotide sequence ID" value="XM_017686169.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C142"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004640"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006671.2">
        <property type="protein sequence ID" value="ENSPNAP00000004640.2"/>
        <property type="gene ID" value="ENSPNAG00000033933.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413578"/>
    <dbReference type="GeneTree" id="ENSGT01050000244806"/>
    <dbReference type="OMA" id="YCPQNPS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21063:SF4">
        <property type="entry name" value="CD48 ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21063">
        <property type="entry name" value="LFA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043736192" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="135"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004640.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="313" mass="35250" checksum="033602E2D4677401" modified="2024-11-27" version="2">MFSEYRIHPALLHLLYCLLCSAGEEVVRLQELEGNTVTIHTGLTGVQSDAQILWFYISESVDIKIVNSQIIRGEIITDYYSERFRDRLQLNRTSGSLTIRNINREDSGVYKLQIITGHQSVWSFSVDVYAPVSKPVIRNQAEKRSVSLRESCSPLCSVENGKDVSLSWYEEKERISSISSSDSSERLYLPLNKTDPNNSTYTCVAANPVSTQTTQLNITNICCKAASGQNVKRDRFILLAAVLTSVGLLVVPSLLFVWFCKKKKRKQDSEDAELNYTEVKTLTRNSRAAPEINRAGVAEEDHSGSVDYTEIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6K3</accession>
    <name>A0A3B4C6K3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Hedgehog protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <molecule>Protein hedgehog N-product</molecule>
        <text evidence="25">The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development.</text>
    </comment>
    <comment type="function">
        <molecule>Protein hedgehog</molecule>
        <text evidence="25">The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>glycyl-L-cysteinyl-[protein] + cholesterol + H(+) = [protein]-C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:59504"/>
            <dbReference type="Rhea" id="RHEA-COMP:12707"/>
            <dbReference type="Rhea" id="RHEA-COMP:15369"/>
            <dbReference type="Rhea" id="RHEA-COMP:15374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:65250"/>
            <dbReference type="ChEBI" id="CHEBI:143135"/>
            <dbReference type="ChEBI" id="CHEBI:143140"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:59505"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with BOC and CDON. Interacts with PTCH1. Interacts with glypican GPC3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Multimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Sonic hedgehog protein</molecule>
        <subcellularLocation>
            <location evidence="25">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="25">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="subcellular location">
        <molecule>Protein hedgehog N-product</molecule>
        <subcellularLocation>
            <location evidence="25">Cell membrane</location>
            <topology evidence="25">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 25">Belongs to the hedgehog family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547153.1">
        <property type="nucleotide sequence ID" value="XM_017691664.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C6K3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006189"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003991.2">
        <property type="protein sequence ID" value="ENSPNAP00000006189.1"/>
        <property type="gene ID" value="ENSPNAG00000012235.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423966"/>
    <dbReference type="CTD" id="619520"/>
    <dbReference type="GeneTree" id="ENSGT00940000164444"/>
    <dbReference type="OMA" id="APAVRGC"/>
    <dbReference type="OrthoDB" id="5212at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:peptidase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:transferase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell fate specification"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell-cell signaling"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:intein-mediated protein splicing"/>
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        <property type="term" value="P:protein autoprocessing"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Hint"/>
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        <property type="entry name" value="Indian hedgehog"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Indian hedgehog"/>
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        <property type="entry name" value="Hedgehog_Signaling"/>
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        <property type="entry name" value="Hedgehog_signalling_dom"/>
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        <property type="entry name" value="Hint_dom_C"/>
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        <property type="entry name" value="Hint_dom_N"/>
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        <property type="entry name" value="Hint_dom_sf"/>
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        <property type="entry name" value="Intein_N"/>
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        <property type="entry name" value="Zn_M74/Hedgehog-like"/>
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    <dbReference type="PANTHER" id="PTHR11889">
        <property type="entry name" value="HEDGEHOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11889:SF39">
        <property type="entry name" value="INDIAN HEDGEHOG PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01085">
        <property type="entry name" value="HH_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01079">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009400">
        <property type="entry name" value="Peptidase_C46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00632">
        <property type="entry name" value="SONICHHOG"/>
    </dbReference>
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        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="Hedgehog/DD-peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 25" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="15 24" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 25" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 25" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 25" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16 25" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 25" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="17 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7 25" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 25 26" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14 24" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="413"/>
        </location>
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        <location>
            <begin position="196"/>
            <end position="308"/>
        </location>
    </feature>
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        <location>
            <begin position="311"/>
            <end position="355"/>
        </location>
    </feature>
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        <location>
            <position position="89"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
            <position position="90"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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    <feature type="binding site" evidence="24">
        <location>
            <position position="95"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
            <position position="125"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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        <location>
            <position position="126"/>
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            <name>Ca(2+)</name>
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            <label>1</label>
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        <location>
            <position position="126"/>
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        <ligand>
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            <label>2</label>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
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    </feature>
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        <location>
            <position position="131"/>
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            <name>Ca(2+)</name>
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            <label>2</label>
        </ligand>
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        <location>
            <position position="140"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <location>
            <position position="147"/>
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        <ligand>
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        <location>
            <position position="182"/>
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            <name>Zn(2+)</name>
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        </ligand>
    </feature>
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        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="site" description="Involved in cholesterol transfer" evidence="23">
        <location>
            <position position="246"/>
        </location>
    </feature>
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        <location>
            <position position="269"/>
        </location>
    </feature>
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        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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            <dbReference type="ARBA" id="ARBA00010649"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022670"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022813"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034131"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00048589"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR009400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU280812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="SMART" id="SM00305"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="413" mass="45318" checksum="AB2023B9C175B239" modified="2018-12-05" version="1">MRLLALLGLLASCALLLAPAAQACGPGRGYGKRRPPKKLTPLSLKQFSPNVAEKTLGASGRLEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNSLAISVMNMWPGVKLRVTEGWDEDGNHFDDSLHYEGRAVDITTSDRDRNKYGMLARLAVEAGFDWVYYESKAHVHCSVKSEHSVAAKTGGCFPGGALVTLEDGSRKAMRELQPGERVLASLQSDGNGQLLYSEVIAFLDRSPAARQQFYTLVTDSGATLSLTAAHLVFVAEGNCSGPVASSQLQTVFASQVQLGQCVVWARGAGQEGHLSRVSRIKMKQDTGLFAPLTRHGTVVVNDIVSSCYAATDQHRLAHWAFGPLRALHSWGGPMGHQAEGVHWYPSLLHWVGSRILNPKHFHPWGMMDIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6S4</accession>
    <name>A0A3B4C6S4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KIF17</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Dendrite-specific motor protein which, in association with the Apba1-containing complex (LIN-10-LIN-2-LIN-7 complex), transports vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Interacts with APBA1 (via PDZ domain); the interaction is direct and is required for association of KIF17 with the cargo that is to be transported. Interacts with IFT B complex components IFT52 and IFT57. Interacts with IFT70B. Interacts with PIWIL1. Interacts with TBATA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C6S4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007567"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001619.2">
        <property type="protein sequence ID" value="ENSPNAP00000007567.2"/>
        <property type="gene ID" value="ENSPNAG00000013334.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158776"/>
    <dbReference type="OMA" id="CESIMEM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:microtubule motor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:cell projection organization"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:eye photoreceptor cell development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:microtubule-based movement"/>
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        <property type="term" value="P:photoreceptor cell outer segment organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000029">
        <property type="entry name" value="Kinesin-like protein KIF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
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        <property type="entry name" value="Kinesin_motor_dom"/>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF21">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="5"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="451"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="689"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="398"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="581"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="451"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="689"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="91"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059114"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007567.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="802" mass="89941" checksum="8C55C6ACE38E10EE" modified="2024-11-27" version="2">MASESVKVVIRCRPLNDREKVLSCKLVVSVDASHCQCFIEKPGAAEEPPKQFTFDGTYFINQTTEQMYNEIAYPLVEGVTEGYNGTIFAYGQTGSGKSFTMQGVADPPAQRGVIPRAFEHIFETIQCSEDTKFLVRASYLEIYKEEIRDLLGKDTKQKLELKEHPERGVYVRDLSMHTVHSVAECERIMEIGWKNRSVGYTLMNKDSSRSHSIFTIHLEICNTDAAGKDHLRAGKLNLVDLAGSERQSKTGATGDRLQEATKINLSLSALGNVISALVDGRSKHIPYRDSKLTRLLQDSLGGNTRTLMVACLSPADNNYEESLSTLRYANRAKSIQNRPRINEDPKDALLREYQEEIKQLRALISGQLGTASLNLSALLASQKSEAALALQSRPQSSTVESEAEKEKIKQEYEEKLARLQADYNAEQESKAKLQEDITVLRTSYEIKLSSLESSKASRAHTTADNSCIEEVSPLNGSTPEPSHAAQEEIKEDECSQESSIRVSPGGEPVVVATPGPLDQKHVLERLQQLEQEFVGGEQARNEELRQRRRQRKTLANQRKKQLIEALRQGSEDGDSVLLNVYDSIQEEIHAKNKLLENMQNKLKAAKLEIRDLQAEFETERDDYLATIRRLERETQLQQAILERMVGLVRRDCNYSNLDRLRKEAVWDEESSVWRLPEVLVQKTALPAVPPSAVSSSSRLPARRNSASDSAEPFMEQEDRYKEMLNRSDSEHIASNYFRKKRTTPLFAGDASNGPSHQTSSSSNLAAEALLPRPFRLESLGVLPVSAKAKRKKSKTQILCDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C736</accession>
    <name>A0A3B4C736_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D(1B) dopamine receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">D(5) dopamine receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Dopamine D5 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DRD5</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C736"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006935"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002685.2">
        <property type="protein sequence ID" value="ENSPNAP00000006935.1"/>
        <property type="gene ID" value="ENSPNAG00000012878.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155857"/>
    <dbReference type="OMA" id="IMVCSAV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001588">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gs"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000691">
        <property type="entry name" value="Predicted vertebrate-like dopamine D1-like G-protein coupled receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000497">
        <property type="entry name" value="Dopamine_D5_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF136">
        <property type="entry name" value="D(1B) DOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00566">
        <property type="entry name" value="DOPAMINED1BR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="31"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="464" mass="51248" checksum="675FF06ACFC76A43" modified="2018-12-05" version="1">MWNGTEADARPALRALTGCVLSALILWTLLGNVLVCAAVLRFRHLRTKVTNTFIVSLALSDLLVAVLVMPWKAAAEVAGYWPFGAFCNIWVTFDIMCSTASILNLCVISVDRYWAIASPFRYERKMTQRVAFVMIGVTWTLSVLISFIPIQLNWHKAHADSTRSTSSTADMDSPEEHAGAPAREDCDSSLSREYAISSSLVSFYIPVAIMLVTYTRIYRIAQTQIRRIASLERAAEHAQSCRAACREHQQLQQQHHHQNHTSTLRTSISRETKVLKTLSVIMGVFVCCWLPFFVLNCVVPFCARTSPGGGGAGSGARRSGDASAGACVSDTTFDVFVWFGWTNSSLNPVIYAFNAEFRKAFASLLGCRRLCTSTPVETVNISNELVSYNQDTLLHKEIASAYVNIIPNVVECAEREDTFDRISQLSRNNDDLDDLATDSACELDRSAADISLDALAPFTPNGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8G5</accession>
    <name>A0A3B4C8G5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear speckle splicing regulatory protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 55</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NSRP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSRP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559765.1">
        <property type="nucleotide sequence ID" value="XM_017704276.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8G5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007948"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000977.2">
        <property type="protein sequence ID" value="ENSPNAP00000007948.1"/>
        <property type="gene ID" value="ENSPNAG00000013616.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431257"/>
    <dbReference type="CTD" id="84081"/>
    <dbReference type="GeneTree" id="ENSGT00940000154049"/>
    <dbReference type="OMA" id="MSKPLAF"/>
    <dbReference type="OrthoDB" id="446635at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042816">
        <property type="entry name" value="Nsrp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018612">
        <property type="entry name" value="NSRP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31938">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31938:SF4">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09745">
        <property type="entry name" value="NSRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear speckle splicing regulatory protein 1 N-terminal" evidence="6">
        <location>
            <begin position="58"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="264"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="472" mass="55918" checksum="DBBDD3D5CB3D819E" modified="2018-12-05" version="1">MAAARGKQYGLILPQKSTSKSASLPRPSIFEDDSDEETSVGETLQKEAIKKKMMKQTRLEMQKALEEDSSVYEYDNVYDDIQKQKFESNKKLLGGTDKKPKYINQLMHAVEERKKEQERREERKVQKEREAEGEKFADKEAYVTSAYRQKLKERQEELEKERRAAEIEAALDVKKQKDLSGFYRHLLNQTVGQEAIPDPSAERPDKSKEEKSPVVNSPQRANDSEEEPHTDEDQRTSKPEFSKTSSNSSHSRRHYRQRSPSSGSDEREERKPDKEKRCHKEKERDRARDRDREDKHGHRKDDRDHRGERDRHREREDDRGRDRRDREDDRGRERRDREDDRGRERRDKERDKEDRHGKRNRAEGSPKDREHDRNEEKGRERDRHKERDHHKTDDKEKRKDAEKEREKKTQGEGKEKEKGAQAENGTEGEAAQAPSKFAKRSSEQTVSSARERYLARQMARSAAKPYIEKEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAI3</accession>
    <name>A0A3B4CAI3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAI3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008618"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014710.2">
        <property type="protein sequence ID" value="ENSPNAP00000008618.1"/>
        <property type="gene ID" value="ENSPNAG00000014229.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286997"/>
    <dbReference type="OMA" id="KCTRFNF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF0">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017369437" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="24"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="594"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="664"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="708"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="753"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="788"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="820"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="594"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="864" mass="95208" checksum="FA6B5AE42A3C88CC" modified="2018-12-05" version="1">MQWWGVYACLCAPVAFLCTLISAQLLQQQTCRLIRPSRLPTVAKEGNITLGALFSLHDAVLESELSFTSQPEPAQCTGFNFRTFRWMQTMIFAVEKINRDSRLLPNITLGYKIYDSCSSPLHALRTAVELMGGREEESDGTECQGKVPVVIGDGGSTLSLVVAHFLGVFQLPQVSYFSSCACLSNKKAFPAFLRTMPSDFFQVNALAQLVQHFGWTWVGTVAGDDAYGRGGAQIFNDKVTKLGACIGLYEIIPKNHAPAEMARIVQRIGGSGTRVVLVFALEQDAKALFSEALKHNLTGVQWLASEAWITAAILTTPEFHSILQGSMGFAIRRAEIPSLQPFLLRLHPSSFPDDPFVLQFWEEVFRCSLQNGSKGVGSDALRPCNGSEDLASVKSIYSDVSQLRISYNVYKAVYAIAHALDAMLRCVPERGPFPGGACPDTLSIRPWQLLYYLKKVDFINELGEETKFDVNGDPVAMYDLINWQHLASGEVQYATVGRFDEIRSANLEIEEENIIWNGYQRQVPVSVCSSSCPPGTRKAIRPSFPVCCFDCILCAAGEINAVECATCPPEFWSNLKRDACIPKLVEFLSYGDTMGMALLAVALLGSCATLVIGLVFACKRHTPLVRANNSELSFLILSSLWLCFLCALAFIGQPTAWSCQLRHTAFGIAFCLCLSCILGKTMVVLMAFKATLPGSNAMRWFRPPQLRAVIFLCTAVQVGICGAWLGLAPPFPRRLMTRESARIILLCDMGSTLAFSLVLGYIGLLAAVCFLLAFFARKLPDNFNEAKFITFSMLIFCAVWIAFVPAYVSSPGKYTVAVEVFAILASSYGLLLCIFAPKCYVILLRPEKNTKKHLMGNSMTEKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAY6</accession>
    <name>A0A3B4CAY6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GTPase IMAP family member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Immune-associated nucleotide-binding protein 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Exerts an anti-apoptotic effect in the immune system and is involved in responses to infections.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540704.1">
        <property type="nucleotide sequence ID" value="XM_017685215.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAY6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009102"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015362.2">
        <property type="protein sequence ID" value="ENSPNAP00000009102.1"/>
        <property type="gene ID" value="ENSPNAG00000014663.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412961"/>
    <dbReference type="GeneTree" id="ENSGT00940000166743"/>
    <dbReference type="OMA" id="GHICANM"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000536">
        <property type="entry name" value="GTPase IMAP family member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF188">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="10"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="343" mass="37366" checksum="89A2467DF2514692" modified="2018-12-05" version="1">MEHVTESADRHEVRLMVIGKTGAGASASGNTLLGHNLFQSARSFSSVTLRCKGETAFVANRRLTVFDTPNFFNSSCPDIHGEVEKGLRSSSPGLHALLLVLTLDTYTKLDADVLTVYKQTFGKEALKYTIVLFTHGDEVQGASVEQLIEQHREISNLVEECGGRYHVLNNKDPSNRAQVTELLEKIDTMVSANENSCYTLEMFLYAHAFTAHFKRTVQAVLQIKPQYVCLVLIVAIGCINMQNKRSGDVMNFGHGCLTGLAAEVVGALSGKAWEVTFTNLSWAARGFNHKCAARIKFLGIPVGSAAGLIVAHYIGPQELSIRVFSGLAGGVAAIMGMSKYIYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CBU3</accession>
    <name>A0A3B4CBU3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CBU3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008361"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000262.2">
        <property type="protein sequence ID" value="ENSPNAP00000008361.2"/>
        <property type="gene ID" value="ENSPNAG00000031072.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="OMA" id="PMICINI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="133"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008361.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="301" mass="33541" checksum="9A18B06E24ACA194" modified="2024-11-27" version="2">MMDPAVMIVLLLYRIGKFHKATIKVFPLVISAEAGEAVNLTLTFVDNSIKDVMMCYKQRLEHAPLEVGSQIEGKSAVISDQLDKSRFKLNRTASGISLSIERITKEDEGMYFCVESGKKMLNFSTATFLALNTSVLQTAVPWSVSPGESVTLQCTVMSEIRAADLRVLWFRAAAGQSFPEIIYTHQNSSSRQCEISSSTSCSLYEFSKNILDHNHTGTYYCAVAVCGKIIFGNGTSVELSKCSCVLLGTCSIFLLFIFKSMICINIKRENRKCDRNRFCNLCCKTTHHTLFIQYSVALTTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEB1</accession>
    <name>A0A3B4CEB1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578806.1">
        <property type="nucleotide sequence ID" value="XM_017723317.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEB1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009848"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016311.2">
        <property type="protein sequence ID" value="ENSPNAP00000009848.1"/>
        <property type="gene ID" value="ENSPNAG00000003379.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442972"/>
    <dbReference type="CTD" id="100006078"/>
    <dbReference type="GeneTree" id="ENSGT01030000234667"/>
    <dbReference type="OMA" id="FWASEAS"/>
    <dbReference type="OrthoDB" id="9942559at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000035">
        <property type="entry name" value="C-C chemokine receptor type 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001718">
        <property type="entry name" value="Chemokine_CCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF664">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00641">
        <property type="entry name" value="CHEMOKINER7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="68"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="370" mass="41475" checksum="0841634D9687ADA6" modified="2018-12-05" version="1">MTVNGKMDIETDPIFDSWTTPDYMSSGTADYDYTDNESGICDKSWVRSFRSSYEPPLYWIIFAVGAVGNLLVVWIYTTVKQRLKTMTDVYLLNLAIADLLFLCTLPFWATDATQGWVFHVTVCKAVSAVYKVNFFSSMLLLTCISVDRYIAIVRVTAAHNFKSRRMFYSKLACLVVWLLSCLLSLPEFLFAKVKENSRGDLSCSLVYPVSSNNLTKVMVLCLQISVGFCLPLLVMVVCYSVIIRKLMQARNFEKHKALKVIFAVVAAFVISQLPYNSHLVVEAAQAYNATMNNCEDVKAFDVAGQIVKSLAYTHCCLNPILYAFIGVRFRKDLLRLCHCLTGMSGVTKAQPVPKRPSVMSDTDTTPALSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CF33</accession>
    <name>A0A3B4CF33_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CF33"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017265.2">
        <property type="protein sequence ID" value="ENSPNAP00000010572.2"/>
        <property type="gene ID" value="ENSPNAG00000016144.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182912"/>
    <dbReference type="OMA" id="RGSHEVM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF25">
        <property type="entry name" value="ZINC FINGER (C2H2)-60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010572.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="107" mass="12061" checksum="36BB7EB1C747F262" modified="2025-10-08" version="3">MAAETMAVFLVRDESGQVGNVGIVIEGSIVLDHLGDLSRGCCYLLGLTYALDLKYPKTLKYIFEVFQKILLEVDPGKLPRKVLRLKNNLMTYCFHRLTVSCYTVTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIZ9</accession>
    <name>A0A3B4CIZ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="6">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF-3-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p35</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">EIF3J</name>
        <name evidence="6" type="synonym">EIF3S1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIZ9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018294.2">
        <property type="protein sequence ID" value="ENSPNAP00000011345.2"/>
        <property type="gene ID" value="ENSPNAG00000017022.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436902"/>
    <dbReference type="CTD" id="394189"/>
    <dbReference type="GeneTree" id="ENSGT00390000018400"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.60:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011345.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="250" mass="28592" checksum="5EF6AD2C35D73327" modified="2024-11-27" version="2">MAEADSWDADSFEPEEPLSQAAAGRDKWEGEDEDEDVKDNWDDEEEEEEKVTETKAVTVVKPTEKKKLGDKIKEKESLQRKRQEELRKQLEESKDSTELTPEEELAEKLRVKKLQEDSDLELAKDAFGIVSNNVTGIDAMSPSSKDDFAEFERLLKDKITSYEKSIHYSSFLESLFRDLCLSLEVEDLKKVNNSLTVLLSEKQRQEKQSKGKKKKKGVLPGGGLKAKMKDDLADYGEFDGGYVQDYEDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJ85</accession>
    <name>A0A3B4CJ85_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">POU1F1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJ85"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011425"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018394.2">
        <property type="protein sequence ID" value="ENSPNAP00000011425.1"/>
        <property type="gene ID" value="ENSPNAG00000017085.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158913"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000150">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF84">
        <property type="entry name" value="NETRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="14">
        <location>
            <begin position="212"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="300"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="302"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="433" mass="48465" checksum="D7EE6CC6843DB49D" modified="2018-12-05" version="1">MLVLLHEPQCRAERHTERERETIMTCQAFSSTDSFTTLSGDSALPLLMHHAGAADCLPVTSHTTNMVQPVPSGLPLVQPSKRSHMHLSTSALSNASAGLHYSMPPCHYSNQQTTYGMMAAQEMLSASISQTRILQTCSVPHPNMVNGANSLQGALAPCLYKFPEHGLGGGSCTLSHSFPPLPQALFTDEPSLGDIKQELRRKSRPPEEPPDMDSPQIRELEKFANDFKLRRIKLGYTQTNVGEALAAVHGSEFSQTTICRFENLQLSFKNACKLKSILAKWLEEAEQAGALFNEKMGMQERKRKRRTTISLGAKEALERNFVEKSKPSSQEIVRMAEGLHLEKEVVRVWFCNRRQREKRVKTSLHHSSLMAKETPACRLCLCHHLLHKTFTTTLQLPGHDDRYRNINGFRLDTVKMPDADTVSLCVRMCVSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CM46</accession>
    <name>A0A3B4CM46_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">LIM homeobox 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LHX6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CM46"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011901"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019025.2">
        <property type="protein sequence ID" value="ENSPNAP00000011901.1"/>
        <property type="gene ID" value="ENSPNAG00000017642.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156868"/>
    <dbReference type="OMA" id="DFGRHIN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021884">
        <property type="term" value="P:forebrain neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09380">
        <property type="entry name" value="LIM1_Lhx6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09382">
        <property type="entry name" value="LIM2_Lhx6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000050">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000023">
        <property type="entry name" value="LIM homeobox 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000031">
        <property type="entry name" value="LIM homeobox 6, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF121">
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    <proteinExistence type="predicted"/>
    <keyword evidence="7 11 13" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
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            <begin position="69"/>
            <end position="129"/>
        </location>
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            <end position="192"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
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            <begin position="36"/>
            <end position="47"/>
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    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="39330" checksum="3F8ABD51BB167273" modified="2018-12-05" version="1">MFWKNELLAPLVEGNQFVSEGISSKQVRNRHNRHASRCDSDSQREPPRSPSPSSSPSSPPSSASSSSRNVCASCGLEILDRYLLKVNNLIWHVRCLECSVCRTSLRQHSSCYIKNKEIFCKMDYFSRFGTKCARCGRQIYASDWVRRARGNAYHLACFACFSCKRQLSTGEEFGLVEEKVLCRIHYDTMVENLKRAAESGSGLTLEGAVPSEQDSQPKPAKRARTSFTAEQLQVMQAQFAQDNNPDAQTLQKLADMTGLSRRVIQVWFQNCRARHKKHTPQHNGPPQGHPQARMPPSLPDELHYSPFGSPERARMVALHGYIDSHPFSVLTTQSLPHQAMSLPQLPLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMZ3</accession>
    <name>A0A3B4CMZ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="4 15">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="4 15"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012728"/>
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        <property type="protein sequence ID" value="ENSPNAP00000012728.2"/>
        <property type="gene ID" value="ENSPNAG00000018519.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="C2_PLC_like"/>
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    </dbReference>
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        <property type="entry name" value="EFh_PI-PLCeta"/>
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        <property type="entry name" value="Phosphoinositide phospholipase C"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF146">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="233"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="18">
        <location>
            <begin position="607"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="722"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="505"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="890"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1120"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1282"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1353"/>
            <end position="1390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1402"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="507"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="534"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="549"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="934"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1125"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1283"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1354"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1371"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1402"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1422"/>
            <end position="1437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012728.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1486" mass="168361" checksum="23E34EE8400ABEC7" modified="2024-11-27" version="2">MNTSPAMAPLSPVIFSPCSPSRTPHRPPSPALMTSPKLWQKASISRLAEEFFWIGGIIVATPKWRIGQFVERCMCTMQTGTQMIKLRGKSKTLVRFFYLDEHRSCIRWRPSRKHDKAKISIDSIHEVCEGKKTEMFQHFHSSKFDSNCCFSIYHGNHVESLDLVTTNGEEARTWITGLKYLMAGISDEDCLARRQRTRDQWLRQTFSEADKNGDGNLSIGEVLQLLHKLNVNLPRQKVREMFEEADTDDNQGCLAFEEFCNFYKMISTRLDLYLIMLTYSHRKEHMDITDLIRFLESEQKMVNVSREYCQMIVNQFEPCPENRAQLVLGIDGFTNFMRSPPGDIFNPEHYQVNQDMTQPLTNYFIASSHNTYLTGDQLLSQSRVDMYAYVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFRDVVETINKYAFAKSQYPVIISIENHCSVPQQKKMAQYLTEVLQDKLDLSSVNINESRRLPSPEVLKGKILVKGKKLPANIDADAEEGDVSDEDSGEDEEEEEEEEETNEENRADGAEAGEETKKRSRRFMGSFRRKKKKRVKSKKRPVLSDSDTDQEVPSSNPNKVINYGKRRKTMRLSRALSDLVKYTKSVRVHDIETQAYMSSWQVSSLNESITNQILQFKPAQLVRFNQRQLLRVYPSNYRVDSSNFNPQPFWNAGCHLVALNYQTEGRMLQLNRAKFAANGNCGYVLKPKCMWKGAFNPMLTDPLPGHRKTQLVLKIISGQQLPKPKDSMLGDRGEIIDPFVEVEIIGLPVDCNKEQTRVVDDNGFNPMWEETLVFTLLMPKIALVRFMVWDHDPIGRDFIGQRTIAFTSMMPGYRHVYLEGMEEASIFVHVAINDITGKNKSSNAVKQLIQKNLMQTSEDGTRTSFAPNFLKKSGAGKSQAKSKEVSQESYSREDFLNRRYSDDVNSQDSRTASQSHSLHRGAQSEPLKRSHRVCFQEPEERLGVTQSHLSSTDSCGESERLSNYVNLGFSPRENPEVNPTVQRGNSKLRVELSQSHFPSINSREESGSTAIYVNLGFNPRENPEVNPPAQTKQNKLIVGVSQNPTSTGSWGALGRSSNYINLGFDSRESPDLSTSSQKDQSKFKLEVTRRHFSTSDSCGASENASNYVNLGFDPREKPELSPNQAKLSHQEDAISKASDLPPLVRKEDADADPTLSLEPEIKVLAPQRPDRPSLSKQSSVVEKVFKDPEPECERYQPALDQEIDSFSRHRAFKNVGLQGALTSFKLQPISDHKPETCTEIWTPKSQSIGVGHQKECKSSKFDPEENRYTKHKGEQRTLSSPRLTTVKSGFGLRSSVNIQAKPANESLSILATPAMNKQVLYNRGSQSSTESPSAQITQLHRSHSIPRRRPLTPPNTQHNFKTDVWKYSLPQNNISPVPQESSAPDLPNNHGLSESDSSSEGSLNSLDLSTLPCRLPVSQQQNVGTLQREMNALFEQKMTEIRCHSPLFFSDHSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPD8</accession>
    <name>A0A3B4CPD8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SRSF11</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPD8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012661"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020000.2">
        <property type="protein sequence ID" value="ENSPNAP00000012661.2"/>
        <property type="gene ID" value="ENSPNAG00000018440.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000110872"/>
    <dbReference type="OMA" id="MINNHRN"/>
    <dbReference type="OrthoDB" id="7763451at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000084">
        <property type="entry name" value="Serine/arginine-rich splicing factor 11 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF6">
        <property type="entry name" value="SERINE_ARGININE-RICH SPLICING FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="6"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012661.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="447" mass="50884" checksum="4C19EE22708AE16E" modified="2024-11-27" version="2">MNCSTNVVQVTNVSPSTTSEQMRTLFGFLGTIEELRLFPPDDSTMPVTSRVCFVRFQEQESVGVAQHLTNTVFVDRALIVVPYAEGVIPDESKALSLLAPANAVAGLLPGGGLLPTPNPLASMGGVPLGALGGPAILPGAGMNPQALSAEQLLKLMSSVDPKMNPMAASMGLKADASSKEIEEAMKRVREAQSLISAAIEPGNKKDSKRKYSRSRSRSRSRRRRSRSRSRHKRSSSRSRRRSRSRSRRRSRSPRRRRSRSRDRNRRSASRSRDRKKEEKDKKRSKTPPKSYSTARRSRSTSRRRRKSVSRSPRRSPKRRVSRSPSPRKHKKEKKRDREREKDRERDRDKRTERERSSSKKKKSKDKERERTSDSEKRDVKVLLKVTRDYDEEEQGYDSEKEREMKDSEDSGSSPARYADDGEGKHSPKQSKQNGDDHHEEQDMEVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRC0</accession>
    <name>A0A3B4CRC0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Vitamin K-dependent protein C</fullName>
            <ecNumber evidence="6">3.4.21.41</ecNumber>
            <ecNumber evidence="29">3.4.21.69</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="33">Anticoagulant protein C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Autoprothrombin IIA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Blood coagulation factor XIV</fullName>
        </alternativeName>
    </protein>
    <organism evidence="47 48">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="47 48" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="47" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="47" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="28">Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.</text>
    </comment>
    <comment type="function">
        <text evidence="34">Serine protease component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1R catalyzes the first enzymatic step in the classical complement pathway: it is activated by the C1Q subcomplex of the C1 complex, which associates with IgG or IgM immunoglobulins complexed with antigens to form antigen-antibody complexes on the surface of pathogens. Immunoglobulin-binding promotes the autocatalytic cleavage and activation of C1R. Activated C1R then cleaves and activates C1S, the second protease of the classical complement pathway. It is unclear if C1R activates C1S within single, strained C1 complexes or between neighboring C1 complexes on surfaces.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>Degradation of blood coagulation factors Va and VIIIa.</text>
            <dbReference type="EC" id="3.4.21.69"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).</text>
            <dbReference type="EC" id="3.4.21.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="35">Core component of the complement C1 complex, a calcium-dependent complex composed of 1 molecule of the C1Q subcomplex, 2 molecules of C1R and 2 molecules of C1S. The C1Q subcomplex is composed 18 subunits: 3 chains of C1QA, C1QB, and C1QC trimerize to form 6 collagen-like triple helices connected to six globular ligand-recognition modules. Within the C1 complex, C1R is a dimer of identical chains, each of which is activated by cleavage into two chains, heavy and light, connected by disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="37">The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.</text>
    </comment>
    <comment type="caution">
        <text evidence="40">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.41" evidence="6"/>
    <dbReference type="EC" id="3.4.21.69" evidence="29"/>
    <dbReference type="RefSeq" id="XP_017555935.1">
        <property type="nucleotide sequence ID" value="XM_017700446.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CRC0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014637"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022595.2">
        <property type="protein sequence ID" value="ENSPNAP00000014637.1"/>
        <property type="gene ID" value="ENSPNAG00000020578.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429008"/>
    <dbReference type="GeneTree" id="ENSGT00940000157473"/>
    <dbReference type="OMA" id="WINKTMS"/>
    <dbReference type="OrthoDB" id="6261922at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
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    <sequence length="563" mass="62975" checksum="EB41C2D9F417DE15" modified="2018-12-05" version="1">MDWSLNLIWLLVIYMNIQSFAFQPVMYGEVQSPHYPEHSPSPLHEHWDLEVPKGYQIQLNFSYLNIKLSTGCRNDFFKIVHNGTVEKFCGEGNRSISIPTSNVRLSLQTSEVDKGPYPPVGFSAFYHAEDVDECLGNRTDLPCSHICLNTMGSYLCACPHGFRLKSNQHTCVEVQCESPNNINGRITPTLSTYYYNNVTTVHCDEGYRINTEGGEVSMHQFTCQSNGQWSMPLLRCHITYCGAVPALLNGKVLHISGAHNQYRSVIKLHCNEPYALPATQNATFTCYADKKWKDDHNRVMPVLHKCLPVCGRPSAALTRHKRVLGGQLASQRAFPWHVFMHQPVRGGGAIISEQWILTAANNLMSNNQPVSPDKVKIHIGGNDALQLSSMPPLQIESLHIHPLYNNPDFTNFDHDLALIKLKNTIQYNADVMPLCLPSLEAEYTPSLTGWVSGFGQTARSKISRYLRYTSLPLVNKEMCQKFIDNLRIPVPLTDNMFCAGVPKGGKDACGGDAGGAFVLKQNGVFYAAGIVSWGYECGKPGRYGVYTRVAQYVDWINKTMSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVL5</accession>
    <name>A0A3B4CVL5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SUEL-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TSHB</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVL5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015425"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037716.2">
        <property type="protein sequence ID" value="ENSPNAP00000015425.2"/>
        <property type="gene ID" value="ENSPNAG00000021458.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154285"/>
    <dbReference type="OMA" id="FIWHIAV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22836">
        <property type="entry name" value="Gal_Rha_Lectin_RBL_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22827">
        <property type="entry name" value="Gal_Rha_Lectin_SUL-I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000003">
        <property type="entry name" value="Protein eva-1 homolog C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043400049" description="SUEL-type lectin domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="6">
        <location>
            <begin position="28"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="6">
        <location>
            <begin position="136"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015425.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="229" mass="25984" checksum="37EB052849E3D38A" modified="2024-11-27" version="2">MYKLQMIWISSIAGAHAASVVAAKRYLTCEGHSAHLSCDYGYIKVLRANYGRTDKTTCSEKRPHHQLSNVHCFQERSLKIMSDRCNGLQSCAVPVVNSVFSDPCVGIYKYLDVFYFCLPYREPYSQICLCLLIVLCTKASGVIFIHYANYGRRDRTTCPHKLATTSDCYFPQTSSLRSRCNGKTSCKLKASNDVFSDPCYGVHKYLEVTYSCRYFVSIQSLIPTFRTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CW16</accession>
    <name>A0A3B4CW16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dehydrogenase/reductase (SDR family) member 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000015533"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023763.2">
        <property type="protein sequence ID" value="ENSPNAP00000015533.2"/>
        <property type="gene ID" value="ENSPNAG00000021589.2"/>
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    <dbReference type="OMA" id="MALELMM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44147">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44147:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015533.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12673" checksum="34BC47F91CD12A04" modified="2024-11-27" version="2">MKTLEQTAAEVNERGGRCLPVICDSTKDEEIKELFERVQREQNGRLDILVNNVYSGVQAIFDNMENSFWEIEPIIWDDTNNTDSTWCAHIKPACGCYGLHLGFLLILFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D111</accession>
    <name>A0A3B4D111_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TNRC18</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017558658.1">
        <property type="nucleotide sequence ID" value="XM_017703169.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391331.1">
        <property type="nucleotide sequence ID" value="XM_037535434.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000016539"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038166.2">
        <property type="protein sequence ID" value="ENSPNAP00000016539.1"/>
        <property type="gene ID" value="ENSPNAG00000022709.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430589"/>
    <dbReference type="CTD" id="84629"/>
    <dbReference type="GeneTree" id="ENSGT00940000157099"/>
    <dbReference type="OMA" id="LHDGKHC"/>
    <dbReference type="OrthoDB" id="6426227at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04714">
        <property type="entry name" value="BAH_BAHCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20469">
        <property type="entry name" value="Tudor_TNRC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052429">
        <property type="entry name" value="BAH_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048924">
        <property type="entry name" value="BAHCC1-like_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056841">
        <property type="entry name" value="TNRC18_BAHCC1-like_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12505">
        <property type="entry name" value="PHD FINGER TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12505:SF21">
        <property type="entry name" value="TRINUCLEOTIDE REPEAT-CONTAINING GENE 18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21744">
        <property type="entry name" value="BAHCC1-like_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24912">
        <property type="entry name" value="SH3_TNRC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAH" evidence="2">
        <location>
            <begin position="2765"/>
            <end position="2910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="950"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1117"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1189"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1492"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1650"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1684"/>
            <end position="1792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1807"/>
            <end position="1845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1913"/>
            <end position="2078"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2090"/>
            <end position="2121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2275"/>
            <end position="2383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2413"/>
            <end position="2442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2471"/>
            <end position="2512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2524"/>
            <end position="2719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="950"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="979"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1026"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1279"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1515"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1595"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1652"/>
            <end position="1661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1689"/>
            <end position="1700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1775"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1826"/>
            <end position="1842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1943"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2013"/>
            <end position="2022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2090"/>
            <end position="2103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2104"/>
            <end position="2116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2276"/>
            <end position="2296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2307"/>
            <end position="2322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2349"/>
            <end position="2360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2486"/>
            <end position="2496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2538"/>
            <end position="2549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2550"/>
            <end position="2562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2582"/>
            <end position="2620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2621"/>
            <end position="2633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2634"/>
            <end position="2648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2666"/>
            <end position="2687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2688"/>
            <end position="2701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2916" mass="319097" checksum="421CF8440ABFD18F" modified="2018-12-05" version="1">MMDGRDFGPPRSVHVPPPLLSGLAMESHRLGAAAAPGRVPPSPGHLGAGHPAPLHTGKFLSPAINIQSHHSDAFPGASSFLGGYVSSSSPHAGPSPLPSDPAFRAPNPASLQMAQLWASHSHEGFPHLPSGLYPSPYLPLGHLEHPQLGQHALFDSQKEGFYLAGSVHSQSAMVRSLAAHMPGSLSREKEAMPPHKNSKDLSRELGRDKQPFKADPGHSVQRREREKGREEPRPHSVVDLTLDVKSEDERRTGSSERPIKTAEHAWPFFQQHSPTPGAADTKSKHPHQPSSFSNCRSSSNSGTPTRYQNVEQDRKERDDEHAQPPYDKQKRCDSGATTLGTLHVSYTSASSLQSSPAQHLPPRLVPSGTYPPPHHMPPGLYQLYSAAKEPPAKEHRVTAPTYVPSVEVYDERKGPIQIASQARDNKSDKSRDRMLLQMGGERTPGDHGRSSVRSESPPAHGDIKRDVVREEGSVIRANNLAVKRPPPSDVSTSKSGHSPDARELVSSMPKHMIKTGLEAEGRNQEREIVQRSGPTFSGLDSTVLHSAEAKWKPFELGNYATNHMAALAAQHIHGPRRDEEGKRMYLDPVVAARPAGSRSSPESHGEVSAMQSLIKYSGNFSSEAGSRQVTDNSTPFGGLGNLKPEASQHSRSKAQHLPPQQPGKQLKREPERPESAKSFGRDGNSSQGEAEVRHPPVGIAVAVARQRDSNKSSSVSDRDRPILGGGMKGPGHEEEEQGDERARHRNDRLLSGRLEREQEKVLRESKELADYQLHPPMVSASGLNPNLMVTGGPALAGAGRWPADPTSHLASHPWLPRTGASSMWLPGSPYGLGPPSLHQALPPGYPPTLPGSIPPPYQFARDPQTGQLVVIPTEHLPHYTAEMLERGPPLWSAVYPSAGSSLQHAHQLQLLSHQQLMRQQELYMIQQQAAQVMELQRNAQFVERLKEQRADLEDKTEKRGGEGTKAPVLHVRKPPTRSPTPSTSYRKALTPLPVTPLPSPVTTLKSEDRSKGESTLPQQSYSHPSTPAPHPVSPASASPPPPSPIHPKQETVEVPEKEMLDLQKRSPASFPSMYSEIPPGYPYQSITAPFGSHYSYLLQPAAAAAADADGLAPAVPLQTEGSERLAGPADVKPRHLCSPAPADALQASREPESMEDSSMPLKEEPCLEESKECVVQDGCGQPLHAVAPVPSQDSNIPPEAETCSRAVASPVPPEENEEDQDNKVALSSSGCPGLYQISGRDSDRGPALETAESSITLPEPACTPSSNYTDPCPIDCDTGPSNSPSVHTNGLTSVHDLKEPNSDTPEVLEQSCDNLPLPHGHQPSPASLGMLSEDPMAGMLALVTASELPRAGAITSLTEPLSSGTACFADISLLESSAAEGMALLSQMAELEIQRQQRDISQGVMHYGLESLLEAGRQVLLEAIECLPQVSIRLPRELDPNKKYSWRQKKDEPMFSKSSLEGMDAMEVDYRVRLAELQRRYKDKQRELIRLQRRRDKVEKQQLQQQFHHEDRNRNLSRRGPGRPRKRKHGLCTLSPTLGKLDGKCGKLPRSALYSEDSESGEVLRKRFRTSNREEEEDEEVDVATPKLKRRSKSWNDQEASSSCSHEVPKAGSKKSRVSEQEQLASKLDKALSLTKLGKLNKVSCKFVEGSSGKSRATSGSRFPVLPDIDMRNKMGKSSLSKDLGLFHKSSKGGKSKMATKSKTSDPCLKGKGQRKSPYSPMRSEISSYSNNTDSEEEDSIKEGWPPRSILGRPGSCVQIPSLCSTPSKKKSASSKRVSLSSSSSIKTKQAAKERKQKHFALLLQEAGVSSSEDSFDQEYFTERDGYEDEDDEEEYELDESDGLCSSSLEESGLGLLARFAASAIPSPITSSPLSLVQLEAKQKAKKKEERQSLLGTEFEFTDSETDIKIRKFPSLLHGKRSTPELPLLPPASVLREDSSPSKRGRKPKKPKPPPELNLDFPADGSDEDRWTRRRSERIFLHDAAQANPISPSKPTAAKVARGSKTSPQGSKELPKAKDPKALGKKKRLKESPLHLPLAQFPSTPSEGSAAPPVSPGRKNKVKPKAREQARGKGGAVSKLMESMAADEDFEPNQDSSFSEDENPPPSAQPERPCTPAPRNCVIDKDELQDGLRVLIPMDDKLLYAGHVNTVHSPDIYSVVVEGERGNRPHIYCLEQLLQEAIIDVKPPSVRYLPQGTRIAAYWSQQYRCLYPGTVVRGSPDMDEVDDLITVEFDDGDTGRIPLSHIRLLPPDYKIQCAEPSPALLVACTKRRGRKCSKDVSESKEAAPKSTEDPAPKNRGRGRKPKPKSEPEVPPKEHEKADTSAAPQVQERPLSAQKSGPRPGSKTQPTTTSSPSISITAEGQKKTTIAKTPAKVRSQPQSIYSSALYGKVLSVDLYNDPSASLASFISTNESGKAKPIKRVRKSEDEGAGFGLKSQRKQPQTEILIKLDHEGVMSPKTKKTKALMMMEGQNPTKRDNKAVMGVSYTTVTTSTPAEKTLKPKPKVKPEPEALNVDSVSTYSIRKLGSASEGLVKVKSSGEKKQKKEDQSVSCSSSSSSSSESEGEEERRTLQEKKKAKQDSSSTSSRASSPTSSSSSTSSSSSSAGFNSSSSSSSSSTTSDEESSCSSDDETPVAPQSPSSQEQAPPQDEEEDVEEKLDVKSEPTVTTPKVQKAPPKQQQRQQKQQQKVKKSVGRPKKREGIHLPTTKELAKRQRLPSVENRPKISAFLPARQLWKWFGKPTQRRGMKGKAKKLFYKAIMRGKEMIRIGDCAVFLSAGRPNLPFIGHIQSMWESWGNNMVVRVKWFYHPEETNPGKKLHDKKNWDQLSGRSLPAVLQASNQRKDFMERALYQSSHVDENDVQTISHKCLVVSLEQYEQMIKTKKYQDSEDLYYLAGTYEPTTGMIFNTDGVPVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D212</accession>
    <name>A0A3B4D212_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D212"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026384.2">
        <property type="protein sequence ID" value="ENSPNAP00000017563.2"/>
        <property type="gene ID" value="ENSPNAG00000023830.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012436"/>
    <dbReference type="OMA" id="HKSVERS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21982">
        <property type="entry name" value="HMG-box_HMGXB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025228">
        <property type="entry name" value="DUF4171"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042477">
        <property type="entry name" value="HMGXB4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048016">
        <property type="entry name" value="HMGXB4_HMG-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46584">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46584:SF1">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13775">
        <property type="entry name" value="DUF4171"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="439"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="439"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="157"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017563.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="637" mass="69380" checksum="A0714EE809E6DC09" modified="2024-11-27" version="2">MAFEEIKKKGVMEMSSMEGEMGLVAGRSQREKKRSYKDLLREEEEIAAQDSELFSMGGDSHKKKKKHSDDCYYKDHHGSGPPPHKKKRKSSDRSPSLSSSSSSQPPHSTDTAMGLLQAITSPLATGSDPSPHMHKKPSYPPHSSHPSSKDRKREGSSKSSHSSFSHSHSRPPGSSSSSSKKHSSSSSSSSSKSSLFHGGTGRGEPLTLREAEGLKMKLILSPKEKNEGGGPNEGFSFPPHLSSSSGTAGHHSSSGLKKAGMKKEKERDRTMSSKLPKKKQHSREPLPVVGKEVEVEGHYGGSLGGLGGNSSSSGGELEAGELVIDDSYTHLSKKKKKSKKSKKKKEKEKDRDKDKSSKEKKHSKGGSAAKKGFTGDPSKSHCHSHSLSNHSTSAGMYAMAPPIHSHHHHHHHQSSDHMIEKKKKKEEKDREKHDKDKPKKKTMTAYQVFCKEYRLNINAEQPGLVFGELSKRLAEIWKQLPEKEKQVWKQKAQYLQHKQNKAEATTVKQKSSLLDSKSKISCKAVGLGSGLTSVGRSSLGVALSPVRVPEVDPIDAAAHLQLLGESLSLIGHRLQETEGMVAVSGSLSVLLDSILCALGPLTCLTAQVPQLNGCPRSVLLVQLETVAYIFQFMSVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D427</accession>
    <name>A0A3B4D427_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein KRI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KRI1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561402.2">
        <property type="nucleotide sequence ID" value="XM_017705913.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D427"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017644"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026500.2">
        <property type="protein sequence ID" value="ENSPNAP00000017644.2"/>
        <property type="gene ID" value="ENSPNAG00000023903.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432215"/>
    <dbReference type="GeneTree" id="ENSGT00390000005605"/>
    <dbReference type="OMA" id="WDNYDPR"/>
    <dbReference type="OrthoDB" id="10252032at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="4">
        <location>
            <begin position="509"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="291"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="761"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017644.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="780" mass="92815" checksum="36A80F07EDFD08BE" modified="2024-11-27" version="2">MSELKINKKFAERYGKYRQKEELQKLKDRFGDRDVESSSGSSDSDSGEESEVELDPELERDFYRTLSLLKKKDPKIYQKDARFYAEEAASSGSDERPSTSKKAEKPMFLKDYQRKVILEREGKFDDDDDSEDEEAARQMRERAASPSYIQEQKQLKESFQKFVQDSDEGSDEGQLLKRRTKTQEEKDKEEADYVEWLKGQAELEGRDEVQDMKYLKDYWNNPELDEKESFLRDYILNKGYLDEDEERIPTYDELMQEDVEDSEEEGESFLQKQEDFERHYNFRFEEPGAQQIKTYPRTIATSVRSKDDRRKKKREEVRDRKVKEKERKQQQLRELKNLKRAEIMEKLKTLQKVTGNEQLAFSELDLEGDFDPQQHDQLMQKCFGDEYYGDNEEEKPQFDDADEELEGHWNWDTWTGEVEEGFEEEGEYGEEEYDGEQESFQPNCEDPDFIMDADYDPSQQPLSKKKKRKELKKKKTKDDAPLMGKKKKKKHHFAEVLTKSKPVFDPQEKTFEQYLDEYYKLDYEDIIDDLPCRFRYRQVVPNDFGLTTEEVLEADEKELNRWCSLRKTCMHRTEREELCDVKNYQIKAQNVQKKKQVFTSLYTENDELHSQSDTKVKMGKKRRAQLKKAEMERENTELKEDKNEEAESNQEASAANTADGIAAEALRETLVEEEEDNEEFLVPKKKMKMERGTVKDEKGEAPKEEPRTEKPKWLKKKNRHRGGQLLSRSLKVKMGGREFSGQRLRAYGLNPKRMHFRELYRQKRKEREKKEKLKNKAKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5M3</accession>
    <name>A0A3B4D5M3_PYGNA</name>
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        <recommendedName>
            <fullName evidence="10">Pleckstrin homology and FYVE domain containing 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PLEKHF1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5M3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018825"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028237.2">
        <property type="protein sequence ID" value="ENSPNAP00000018825.2"/>
        <property type="gene ID" value="ENSPNAG00000025287.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160728"/>
    <dbReference type="OMA" id="YEATSND"/>
    <dbReference type="OrthoDB" id="70570at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PH_Phafin2-like"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="PH_domain"/>
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        <property type="entry name" value="PH_domain-containing_F"/>
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    <dbReference type="InterPro" id="IPR000306">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280:SF2">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 2-RELATED"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="35"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="224"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018825.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="34731" checksum="F6094FC227CF1B18" modified="2024-11-27" version="2">MSDQLTFAMENRERILAVENSFGPSGKSLLESGRILVGEGRLMKLCRRCPQPKVFFLFNDILVYGSIVMAGRWNKNQHIIRLEEVQQEDLEDSMEMSNQWLIKTPQKSFYVGAASAEEKQAWMEHIENCRSQQLQRLGLPANAPAVKDFAATWIPDHASAICMRCSERFSVAHRRHHCRRCGFIVCNSCSKARAVLRHISSKPVRVCMLCKVTMEGQERLQRQMKNQVRNQPGKEWKTRSGSNIQYDTSSEEDADELVENSSPTQWFSSQDNSSPYCYFNPDHIKPPRWCCFTTSGLQAHYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6G3</accession>
    <name>A0A3B4D6G3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MPRIP</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000019947"/>
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    <sequence length="1964" mass="223116" checksum="487F6089CEA03079" modified="2018-12-05" version="1">MSAAKENPCRKFQANIFNKSKCQNCFKPRELHLLTDQDLNQAKPIYGGWLCLAPEGTDFDNPMHRSRKWQRRFFVLYEHGCLRFALDESPSTLPQGTVNMNLCTDVIDAEPKTGQKNALCIITPDQEYYIRGDNKEIINGWSEQLVVYPRTNKQNQKKKRKVEPTTSQEPGPAKVAVTGSGIPEAEKVPDSSSIIWQEELHNREADVSQVWSSSEIVPQGSQLPTAAEEASVSRGSDQGSVNGDEVDRSGFSFPPSISQPPQDLLSPTGSSSSRHSAEFGGIPRCLSPAPSDPFPSGSSLLSNGSHISGSMSSLDSDASGSTVTSTESHPTEPLGRALRGLHDPPYSRRLEGEARKAEKRSRLRSPERQSREPLFSPERSGRSNVIEKLEALELENSERMEVEETGRSETRQGRSEMRRFHRQEQKLDPAQSMDFHHTTLPPLRRAKSLDRRTTESVMTPDLLNFKKGWMVKLDEQGQWKKYWFVLTDHSLRYYKDSIAEEASDLDGEIDLSTCYNVTEYQAQRNYGFQIHTQEGVHTLSAMTAGIRRNWIQAVMKNVRPSTAPDVASLTDDHVSCGPFESLARPDITQDSPSSETSSVEREPKKSRARDRRREGRSKTFDWAEFRPIAQALAQQRIHEADALQAQLADPERSKRREERRRRYESVTGSSSLDPAADNSKMDLDSVSVGLGTVSPPSPDRQQKVEDEIEQRWQQVEKTPIREERRVPLSTSLQTRETAELEKLLESYKKGVEDLKAELETCYHHLADSNQHKLDLEDQLKAALDREQQIRAGYISPLESPLSLDTKPQMKRPELVSSQAQSLTKKYQETKELLQLQELKKRNMQAQLGLSLSHLSTKEPYFSDTKNTSTSEILPSEPIVKTVIFELVDNEGKIRELEDLIDSGKPLSVKEMIELLHSHSDSVLSDFPIEKSEDGRSGDITNCQKLLESLKTQQEIENETMRKSLAKAGDSIRNYEARLITMEDMLGRVQRQKMEDLKNPCTSPCQRDESSQMTNRGLAQKVELLTSENEALNQRYQEIVNQLREADREIDRLKAELQRHQSGHQYQLDRENQSMMDQEDKDVLAFKNSYEQELNDKSQKLQEALAKLEVLGHSLKDIEKRLQLKEATLKGLGFQASENGVESLLEKEQLRHHIEVIESKLSEKEKLLQSADQTCKELQAQNEELQTEKQESERNFNQVLLEAKEEIRMLREKGESDNECGVDSDCQAIMQSDEGMIQEVMESFKRRSNALNQVLSMLMKASESKTYDLSSFGIEEETVGRNDRGMVKVLFESEILSRVLDGVENSQHGEGCERTLTVMKNVVKHMLMENRVLVSVMKLSAEQKDIQTPVNMQSTEYGSMLTGEGIVVNDALSGVIMKELTEAVQNKATLLNQIVSVINEPVNELLQSLALTLSSHVSPQGQCFLEAVLDVTHTYLLIGQRIQLENELESSKAVCASCQLKEQNMELELKQSEELSTLQNSVNEDSKLVTHIQIEGEPIDSLDKAIQLQDMVAKHKKELRELREVYEQEASKLRQEVTKAGETLRLRSEENVKEIDSLTICMENLKDKHQMERNILLERFNQEMVELNDALGLHGADLADDGKPNPASLKQQIQKLVLQVSVMTEEMKSQDRGGEAALLRLKYEKDLENLKATCERGFAAMEESHQKVIDELQRKHQRELENLQEEKERLLAEETAATIAAIEAMKNAHRSELEKELDKARKANSNTENADIEEIRRQHEEELLSFQREIEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITKMRSMTSEEGGEAGTAIQGKELYELEVMLRVKESEVQYLKQEINSLKDELQAAQRDKKYATDKYKDIYTELSIVKAKAERDLGRLRDQLQLAHEALGEPTLEEVQRGGYDIMKSKSNPDILKMAAAAAKRSERAMRSKSLREGLTAEQRLHLFDNKDTKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D888</accession>
    <name>A0A3B4D888_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Cholinergic receptor, nicotinic, alpha 2b (neuronal)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565985.1">
        <property type="nucleotide sequence ID" value="XM_017710496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D888"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020567"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040094.2">
        <property type="protein sequence ID" value="ENSPNAP00000020567.1"/>
        <property type="gene ID" value="ENSPNAG00000006668.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434998"/>
    <dbReference type="CTD" id="568849"/>
    <dbReference type="GeneTree" id="ENSGT00940000158299"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19015">
        <property type="entry name" value="LGIC_ECD_nAChR_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000014">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000005">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_lig-bd"/>
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        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
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        <property type="entry name" value="Neur_channel"/>
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        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
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        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022265500" description="Cholinergic receptor, nicotinic, alpha 2b (neuronal)" evidence="21">
        <location>
            <begin position="25"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="23">
        <location>
            <begin position="33"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="24">
        <location>
            <begin position="246"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="414"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="27">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="61741" checksum="161B59E5206DB43F" modified="2018-12-05" version="1">MERAGQIPLRSALLFIILWKCASCYEKTHSHAEDELFKKLFVGYNKWSRPVPNTSDVVIVKFGLSIAQLIDVDEKNQMMTTNVWLKQEWNDYKLRWKPSDYDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMTKAHLFYTGKVRWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLERLETTVDLKDYWESGEWAIINAVGTYNTKKYDCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIIITVFVLNVHHRSPGTHEMPRWVHAVFLELIPRWLFMRRPAPDSRRRQRLLLLQQGQRSRAPGASCLSTSASWFREESRTCCYEDLELGTLSSAFSLTFRPPSPRPPGSTPPPSHGALPRQEGGMTCLPNSRHPGARVNHTKRPTENHQRSPLSPSIMQALEGVHYIADHLRAEDADFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTIGLFLPPWLAGMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8Q8</accession>
    <name>A0A3B4D8Q8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SIL1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8Q8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019344"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029095.2">
        <property type="protein sequence ID" value="ENSPNAP00000019344.2"/>
        <property type="gene ID" value="ENSPNAG00000018906.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286927"/>
    <dbReference type="OMA" id="LRLINCW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="157"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019344.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="581" mass="66116" checksum="F57C0CD670CB9FB3" modified="2024-11-27" version="2">MSNQELQHKVISLVKNQLKRFKKVLSPDFPACSEREVEGEVDQTVREGTLQIALHILRNMNQTDLASTLQTKLAPACHQKIKSTLKKKFQRINEGISYHGGSTILNEIYTELYITEGGSGEVNNEHEVRQIETASRRPATQERPINCNDLFKDTAVRTVLTNGVAGIGKTVSVQKFLLDWAEGKANQDILFIYPLPFRELNSVKSRKLSLVDLLRLFFTDIKQLKPADYYQYKVLFIFDGLDESRLPLDFQNNESLSDVTQSASVDVLLTNLIKGNLLPSALLWITSRPAACGQIPPEYVDQVTEVRGFSDPQKEEYLRKRISDQSVANRVITHMKSSRSLYIMCQIPAFCWIAATVLERMLSEAESGEIPKTLTQMFTQFLIFQMKHKDQKYHGKCDTDPGQTREIILALGKLAFQQLEKGNLIFYEGDLRECGIDVREVSVYSGGCTQIFREELGLHLGKVFSFVHLSVQEFLAALFVFFSFISQNRNVLEHQTHSKSTVTDFLKSAVDRALQSENGHLDLFLRFLLGLSLESNQTLLRGLLTQTGSPSNCREETVEYIKEKLRENLSPEKYINLFYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAZ7</accession>
    <name>A0A3B4DAZ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538522.2">
        <property type="nucleotide sequence ID" value="XM_017683033.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000020623"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040118.2">
        <property type="protein sequence ID" value="ENSPNAP00000020623.2"/>
        <property type="gene ID" value="ENSPNAG00000005100.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411458"/>
    <dbReference type="GeneTree" id="ENSGT00940000162556"/>
    <dbReference type="OMA" id="HTLMENI"/>
    <dbReference type="OrthoDB" id="8964039at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001809">
        <property type="entry name" value="Si:ch1073-365p7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002274">
        <property type="entry name" value="Si:dkeyp-69e1.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF148">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0290503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="31"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="287"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1117"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1839"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1882"/>
            <end position="1907"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="491"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1369"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1117"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1842"/>
            <end position="1851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1853"/>
            <end position="1867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020623.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2108" mass="248037" checksum="279B4BF7E8973AAC" modified="2024-11-27" version="2">METQSENAFNAVKQHAGAKAAGSTQQGYSPLPELRLVLLGRKGTGKSSAGNTILGLNGGFETGKPTEECVKRRADTAGHRVTAVDTPGWEWYYSSNGTPGWVQRETMRSVSLCPPGPHALLLVVRSCASVTEDYFRQIEEHLELLGKTVWGHTMVLFTRGDELGSIPIEQRIQNAGKSFQKLLQRCGNRFHVLENKRRGDDGTQVMGLMRKIEEMVEERGGKHYDSDPLLLVLEVEGKRRARERRKKQRLMEAQTQRGAIKAVLTVDAPHTEDLDERNLFSRGSRRLPELRIILLGERETGKSSAGNAILRAVTFFQTGQATEECSRQQAEVSGRLVTVVDAPGWEGGPDGITPERVRREIGASVTLCPPGPHALLLTLRVDALVRAAAVRDHLELLGEGVWRHTILLFTRGDQLREGVTIEQHIQGGGKDLHWLMEKCGNRYHVISSVCWEGHSSSAQVTGLLEKIEKMVAGNRCEAFSPLVQEIQDLGKQKNERFNQKLKEVNEKLQRQDTELKRMREREVKSIRWFFDRRKDKVKSPEKTSTEKEEVLDKGEDDRKSIMGELEERMAWLTEDKEREIQELSLENSRVIAALNQAYREKDELVMKLEEKDSESDELKEKVDELQVKVLELERVSMLKEQERKEREEELAKRHESVEKEVKMLKEELNQKEKGEEELKRRVEKTEQKMAESKMRYEDEIIQARCETDQILSAKNEFEKRLAEKQKEMEEIKLRAENKIKEQEERWREVSEKREREINTLREMVELMTKEIEELQVAFQEQVKKLEAERQMHENTVIKYNELVDKLLGKDTEILAIQKRQKEQEMAQSVESRAKLQEKDVEINDLTKRHKEKEQEINVLKQAIDAKHKTMEILKNDSQAKMAEFTSRIKLLEDEYKEMEDFLKRENVIIKEEMEIMKKLYEDNMRKKEDDTRQILEMKENMINQLQKQNKDRETHLEDLIEKLRESQIHEGSLQNQNEALMQESEGLLLKCEDYKRDLQAQRAESQRREKEIKDMLNQYEEMGKIKDSLSQEHIEEKEKEICLLKQTNHTQQMDLEDMKERGKELENKIEEMKKYYEERLLEGIAEMDVKDAERERALHSRELELSEREHTLRQHMQELERSKKDVKKQEDDINRREQVLRDEKQVLDKREHEVRDKEKELENQCQNNQRYQEDLERREDELKKREEELLDAEQKLEQEIKKKHEEHKAVEMILESKQKAHKDLSEKQEKEIENIRQQLEERGKDVRKMEDELVLKQLNLREEKKALENHRENVETKEQGLKKRQQQLIDSEHVYENKLQELYRGMQELEKTKQNHEKWQTELTNKEYDLTQRLNELQKKEQELGLITQELMVKSQEIETREQNQATLSDKWKKELEAIELDLSMKEQELRNTKEELERRRQQLADRDKDLDIKEHQINKKYDEYEAAEKMLESKEKAQIISLEKQEKDIEIARQQQEEREKILKHMEEELLVKQIKHKEDLENHTANLDIKESELSKRKQQLMDSEQICEVKAQELSKALQELQNNKQKNEKWQAELTAKEYDLIQRLDELEKKEHQLLQRENDCEVKETNREIEKHQQQLLSLQHNQATDTLETEFEGIKQALAIREQEVTNKEDELQKREDNLRSAEIYLEKREETVQKREEEVQINKQQQDALLCDLKKKQEKTEEIRFSLETKQHELEDLQKELISREQNIATKEQNLSNRYKEQDIKKSTMEKHELDLKSTENTLKKREQELNELKEILEMKKKDLANCTNELESQKQELNREKQVVKVKTEELEKWEKYLYNTELKMINSNSNFKFQPWSQTTAPNGKEQVTQKMYQEVGVDGRTCEKELREYDKDDDGEDDQSVEMASSSKMTNCESENAQLPFQHRSKRELEANTGEEGNGMSLWEEKPGAKEEDGEEFFEPASFDHPVTQGLHSVPQELRVVLLGEMRSSHRSVRHTILGKDTEIGSPWRGSVSGKPLIVVEPQGIKWRSAKAMNVTFEKELMQNVSLCTPGPHAFILILPAYLTFTGQYRRAVECTMAMLGEASWKHAIVLFTWGEALGESGQQHIKRNGDLEWLIEKCGGRYHVLDNRHREAHVVELLEKISKMVAGNPKHYYPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DC23</accession>
    <name>A0A3B4DC23_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Wnt ligand secretion mediator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">WLS</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the wntless family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567779.1">
        <property type="nucleotide sequence ID" value="XM_017712290.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DC23"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020494"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031107.2">
        <property type="protein sequence ID" value="ENSPNAP00000020494.1"/>
        <property type="gene ID" value="ENSPNAG00000006324.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436069"/>
    <dbReference type="CTD" id="79971"/>
    <dbReference type="GeneTree" id="ENSGT00390000005897"/>
    <dbReference type="OrthoDB" id="5804250at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060536">
        <property type="term" value="P:cartilage morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044566">
        <property type="term" value="P:chondrocyte activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035988">
        <property type="term" value="P:chondrocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001958">
        <property type="term" value="P:endochondral ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021986">
        <property type="term" value="P:habenula development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001957">
        <property type="term" value="P:intramembranous ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030917">
        <property type="term" value="P:midbrain-hindbrain boundary development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060872">
        <property type="term" value="P:semicircular canal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061355">
        <property type="term" value="P:Wnt protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047843">
        <property type="entry name" value="WLS-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053936">
        <property type="entry name" value="WLS_GOLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009551">
        <property type="entry name" value="Wntless"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13449:SF2">
        <property type="entry name" value="PROTEIN WNTLESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06664">
        <property type="entry name" value="WLS-like_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21883">
        <property type="entry name" value="WLS_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="429"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="473"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless GOLD" evidence="11">
        <location>
            <begin position="48"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Wntless-like transmembrane" evidence="10">
        <location>
            <begin position="228"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="542" mass="62605" checksum="66104E79CCE077F2" modified="2018-12-05" version="1">MAGAIIENMSTKKLVIVGVILLLFQAFSFMVGGLIAPSPTTAIHYMATKCVDNVKSHYMSKKWFMPWGPNHCDKVRDFDEATAKKIEANNIVFAVHIPMPNKEMSPWFQFMLVILQFDIAFKMYNQIEDGAVVTVDVGLAYRDDSLGEWTEMAHSIEQRKLSCNFTTPKTIDNEGRYYECDLMPLMELGCVFHKYYLLNIRLPVSDHKKVNRGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSILIIMIWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSVGFEWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHLMDQMERNRFSVYWKQVGPIVFGSFCLFIFDMCERGVQLTNPFYSIWASDVGTELAMAFIIVAGICACLYFLFLCFMVFQVFRNISGKRTSLPAMTKARRLHYEGLIFRFKFLMLVTLACAAMTVIFFIISQVNEGHWHWGDYTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKRYGDEQSSGDQVGNSGEDIQLTTTITHVDGPTEVYKMMRKEAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCL4</accession>
    <name>A0A3B4DCL4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">interstitial collagenase</fullName>
            <ecNumber evidence="17">3.4.24.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.</text>
            <dbReference type="EC" id="3.4.24.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="20">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="20">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.7" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCL4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032326.2">
        <property type="protein sequence ID" value="ENSPNAP00000021220.2"/>
        <property type="gene ID" value="ENSPNAG00000004356.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154907"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000002">
        <property type="entry name" value="Matrix metallopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF151">
        <property type="entry name" value="INTERSTITIAL COLLAGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0177">Collagen degradation</keyword>
    <keyword evidence="15 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 25" id="KW-0732">Signal</keyword>
    <keyword evidence="10 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043825895" description="interstitial collagenase" evidence="25">
        <location>
            <begin position="20"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="26">
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            <begin position="109"/>
            <end position="269"/>
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            <begin position="285"/>
            <end position="334"/>
        </location>
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    <feature type="repeat" description="Hemopexin" evidence="23">
        <location>
            <begin position="335"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="23">
        <location>
            <begin position="383"/>
            <end position="431"/>
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        <location>
            <begin position="432"/>
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        </location>
    </feature>
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        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="22">
        <location>
            <begin position="94"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="20">
        <location>
            <position position="96"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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            <name>Zn(2+)</name>
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            <label>1</label>
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            <label>3</label>
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            <name>Ca(2+)</name>
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        </ligand>
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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        </ligand>
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        </ligand>
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        </ligand>
    </feature>
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            <name>Ca(2+)</name>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>5</label>
        </ligand>
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        </ligand>
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        <location>
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            <name>Ca(2+)</name>
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        </ligand>
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            <begin position="288"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
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            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021220.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="473" mass="53543" checksum="FB07927484E3F3A7" modified="2024-11-27" version="2">MKAFYQLCILIGLVIKTHSGPVPLSSILDGRSCQFHQNYLKKLYNMKEEHTRSARSTDHSVSEMSEKLSEMQEFFGLKVTGTLDAETLEVMKKPRCGVPDVAGYNDATGSYKWTKKQLTYRIENYTSDMTQEEVDSSIGRALKVWADITPLRFSRIYSGVADIMISFPVRDHGDGSPFEGEGGFLAHAFVPSPAIGGDAHFDDDETFTFSSSKGYNLFLVAAHEFGHSLGLDHSRDPGALMYPMYSFRDVENFLLPQDDVSGIQALYGPNPDKPERPVPKPPVTPNACDDTLVLDAVTMLRGEIMFFKNAFFWRKQPLSYESEQHLIKSFWPEAPDNIDAAYESPSDDLVYLFKGQKVWALYDYDIAKGFPKTLSSIGLPPKVKKISAALYDQESGKTLFFVNDRYYSYDETTKKMDKGYPKRVEDSFPGMTGKVTAAFQYRDFTYLFSGPRMFEFSYGTLLRVLNNNYFLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEB4</accession>
    <name>A0A3B4DEB4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">complement subcomponent C1r</fullName>
            <ecNumber evidence="4">3.4.21.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">C1R</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Serine protease component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1R catalyzes the first enzymatic step in the classical complement pathway: it is activated by the C1Q subcomplex of the C1 complex, which associates with IgG or IgM immunoglobulins complexed with antigens to form antigen-antibody complexes on the surface of pathogens. Immunoglobulin-binding promotes the autocatalytic cleavage and activation of C1R. Activated C1R then cleaves and activates C1S, the second protease of the classical complement pathway. It is unclear if C1R activates C1S within single, strained C1 complexes or between neighboring C1 complexes on surfaces.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).</text>
            <dbReference type="EC" id="3.4.21.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Core component of the complement C1 complex, a calcium-dependent complex composed of 1 molecule of the C1Q subcomplex, 2 molecules of C1R and 2 molecules of C1S. The C1Q subcomplex is composed 18 subunits: 3 chains of C1QA, C1QB, and C1QC trimerize to form 6 collagen-like triple helices connected to six globular ligand-recognition modules. Within the C1 complex, C1R is a dimer of identical chains, each of which is activated by cleavage into two chains, heavy and light, connected by disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="27">The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.41" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEB4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022757"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014364.2">
        <property type="protein sequence ID" value="ENSPNAP00000022757.1"/>
        <property type="gene ID" value="ENSPNAG00000007292.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183084"/>
    <dbReference type="OrthoDB" id="6261922at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000059">
        <property type="entry name" value="Mannan-binding lectin serine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000016">
        <property type="entry name" value="Mannan-binding lectin serine protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000012">
        <property type="entry name" value="mannan-binding lectin serine protease 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024175">
        <property type="entry name" value="Pept_S1A_C1r/C1S/mannan-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255:SF25">
        <property type="entry name" value="COMPLEMENT C1R SUBCOMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255">
        <property type="entry name" value="COMPLEMENT COMPONENT 1, S SUBCOMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001155">
        <property type="entry name" value="C1r_C1s_MASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="28" id="KW-0106">Calcium</keyword>
    <keyword evidence="18" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="19 26 29" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21 27" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="11 28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 27" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0720">Serine protease</keyword>
    <keyword evidence="12 31" id="KW-0732">Signal</keyword>
    <keyword evidence="9 30" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017379997" description="complement subcomponent C1r" evidence="31">
        <location>
            <begin position="26"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="32">
        <location>
            <begin position="18"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="32">
        <location>
            <begin position="191"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="34">
        <location>
            <begin position="307"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="34">
        <location>
            <begin position="373"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="33">
        <location>
            <begin position="456"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="25">
        <location>
            <position position="494"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="25">
        <location>
            <position position="547"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="25">
        <location>
            <position position="647"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="(3R)-3-hydroxyasparagine" evidence="27">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine; by CK2" evidence="27">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26 29">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="309"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="337"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="375"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="404"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (between heavy and light chains)" evidence="26">
        <location>
            <begin position="443"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="609"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="26">
        <location>
            <begin position="643"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR001155-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR001155-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR001155-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR001155-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="698" mass="77942" checksum="42C8E40F04729605" modified="2018-12-05" version="1">MMEPVISRSAFSLLLLWVSVSVCDCEDVLYGQVQSPLYPDPYPGDNHTQWDLKVPPGYKIQLTFNYFDIEPSADCYYDSLMVLYGRKVLGKFCGQRSTDAHHPGNKPILSPGNHLQLVFVTDDSNPGPQMHLGFSAFYQAVDVDECSSPVPSEDSDPPCSQICLNTLGSYMCACNHGYQLQADGRTCVLDCGGGVYKESEGTLSSPGYPNISPRGLDCVYNISVQPGFEVTLNFTHNFHIEQIYNKGHFCLFHWLLVTVPGKKPMKYCGKKSPGVIHTGSHNVQLEYHTDSAGQSRGWSLHYTTQRVECKLEKSISNGRVTPDFPQYFYRDYIHVRCDQGYKLMMGGKEIKSFKSMCQHNGKWHLPLPECKILDCGDPNPLLNGGVRFLSGSNNEYQSVIQYHCNEPFYAFRTTHSVKFTCAADRRWRANDNNAIIPLCYPVCGRPTVDLSKRDRVLGGKTAPANSFPWQVFLRSGGRGGASIIGDRWLMTAAHNLEHVKFAQEKVQVYVGNIHVSELVKSPSLAIASLHVHPGYKNNNLRTNFDNDIALIKLNSPITFNMNVMPVCLPERDTELNGTGLVSGFGLTEDSLTADTLRYIRLPVVEQDKCHRSIETERKNRHDVAPLTGNMFCAGLPEGGKDTCSGDSGSAFVMKDDETYWVAGIVSWGVDCGQPGKYGIYTRVAKYLNWIQKTMDENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DET0</accession>
    <name>A0A3B4DET0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein ILRUN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ILRUN</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DET0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022005"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033804.2">
        <property type="protein sequence ID" value="ENSPNAP00000022005.1"/>
        <property type="gene ID" value="ENSPNAG00000029253.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043415"/>
    <dbReference type="OMA" id="DPMQTEQ"/>
    <dbReference type="OrthoDB" id="661148at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14349">
        <property type="entry name" value="UBA_CF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000015">
        <property type="entry name" value="Chromosome 6 C6orf106 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000289">
        <property type="entry name" value="Chromosome 6 open reading frame 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039517">
        <property type="entry name" value="C6orf106_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nbr1 FW" evidence="9">
        <location>
            <begin position="82"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="202"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="280" mass="31147" checksum="054DBC2DF8984F03" modified="2018-12-05" version="1">MEAMETDMDLDLVQKFSCMGTTDKDILITEFQRLLGFQLNPAGCAFFLDMTNWNLQAAIGAYYDFESPNITAPSMSFVKDVTIGEGESIPPDTPFTKTWRIQNTGSESWPPGVCLKYIGGDQFGHVNFVMVRSLDPQEMYDVSVQMRSPVAPGMYQGQWRMCTATGLFYGDIIWVILSVEVGGLLGVTQQLSSFQAEFNTQPNRDVQKDYNPFASPQKKKQSGDYGNSLPVGDLKDPMQTEQNGLSNTSVTLAPDNMQSNLSVDSYDQQRTYGPYPFGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEZ5</accession>
    <name>A0A3B4DEZ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho guanine nucleotide exchange factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ARHGEF3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017560356.1">
        <property type="nucleotide sequence ID" value="XM_017704867.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEZ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022075"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033957.2">
        <property type="protein sequence ID" value="ENSPNAP00000022075.1"/>
        <property type="gene ID" value="ENSPNAG00000029400.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431605"/>
    <dbReference type="CTD" id="50650"/>
    <dbReference type="GeneTree" id="ENSGT00940000158385"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061515">
        <property type="term" value="P:myeloid cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10572">
        <property type="entry name" value="PH_RhoGEF3_XPLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000151">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000010">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044129">
        <property type="entry name" value="PH_RhoGEF3_XPLN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006:SF2">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="8">
        <location>
            <begin position="145"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="357"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="474"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="475"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="543" mass="62202" checksum="45F660A9B211C739" modified="2018-12-05" version="1">MQTGVGSNEVSGPMLQRKSSFSLFSSTESWMFRGKKRKQRKRDVDSLSLCSLDINEPSNKRVKPLSRVTSLANLIPPVKAAPLKRIGQTLQRSISFRSESRTEIVPPRPWGRQAPPSITKRRDSKLWSETFDVGLGQMLSSKEIKRQEAIFELSQGEQDLIEDLKLAKKAYHDPMLKLSIMTEHELNQIFGTLDSLIPLHEDLLSRLRQARKPDGSTDHVGHILVDWLPCLDSYNSYCSNQVAAKALLDHKKQDHRVHDFLQRCLESPFSRKLDLWNFLDIPRSRLVKYPLLLREILKHTPNDHPDRQHLDEAINMIQSIVAEINTQTGESECRYYKERLLYLEESQRDMLIDSSRILSCHGELKNNRGAKLHVFLFQDVLVITRAITHSEQLWYQLYRQPIPVRDLEWEDLHDGEIRLGGSIRGAFSNTERTKNFFRVTFRSAGQLQSHSFQASDAFNKQQWLNCIRQAKVAVGSEPPRSPRSPSGLTTPSQYKTEERMDQSDSDSSDCSMDTSDAGAEATEPPTDCPLMEHTDWIATPVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJX3</accession>
    <name>A0A3B4DJX3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Bromodomain containing 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJX3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023234"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013148.2">
        <property type="protein sequence ID" value="ENSPNAP00000023234.2"/>
        <property type="gene ID" value="ENSPNAG00000007783.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157236"/>
    <dbReference type="OMA" id="GMWISTD"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05512">
        <property type="entry name" value="Bromo_brd1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15677">
        <property type="entry name" value="PHD_BRPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20157">
        <property type="entry name" value="PWWP_BRPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000008">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000008">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000007">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042009">
        <property type="entry name" value="BRPF2_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF17">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="211"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="265"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="14">
        <location>
            <begin position="576"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="16">
        <location>
            <begin position="1052"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="71"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="752"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="925"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="512"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="780"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="972"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023234.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1181" mass="133236" checksum="FC14B8D191C894C4" modified="2024-11-27" version="2">MRKRARQPRPSAPQRSPSPIKPSPNRETLTYAQAQRMVDVEVDGRVHRIGIFDKLEVITDDDPTAQEMMECTSNKENTEKPQQAPLRSARLKNSQQKKKAALNAQKPQAPISTLPEPKFRTVDYNLPAVPRRPSQYYKYEEKTSEELDEEVEYDMDEEDYAWLDLVNEKRRSEGFSQVSPNVFEFLVDRFEKESFMESQGQQVQQSMVDEDAVCCICMDGECQDSNAILFCDMCNLAVHQECYGVPYIPEGQWLCRHCLQSPTQPANCILCPNKGGAVKKTEDDRWGHVVCALWVPEVGFSNTVFIEPIDGVANIPPARWKLTCYLCKEKGVGACIQCHKANCYTAFHVSCAQKAGLFMKMEPIKEMTESGTPTFSVKKTAYCGAHTPNGSIRRPLTIYDETKNKTKNGLCQTGKKHSRTRLKKKQKKSKIKKAAEPVAAVPAMSVPSFPPNRLNTILNQVSLQRKKVFVELVLNYWTLKRQSRNGLPLIRRLHSSLQSQKTAQPKHSEKESRALKEQLKEWHRLRHDLERARLLLELIRKREKLKREEMKLQQSILELQLTPFTILLRSVLDQLQEKDQAKIFAQPVSIKEVPDYLDHIKQPMDFSTMRKRIDAQGYKNLEEFEADFNLIIANCMKYNAKDTYFYRAAVRLRDQGGALLRKTRRHVEFIGFESDGGMHSSEPPNVEASAPFSWEEVDRLLIPANRQHMSLDEQLKELLEKLDLTMAMKSSASRSKRLKLLKKTISDVRMEMSLRSSQPLSSEQRRAAVEPSVANGSAEEGDKSIPPKLEPSDSHPPLANADGPSEPPTLKPIEPSLEQPRSGKLDEQQDAFLSHKENLNGHSRDRLLLDDGDVSVVATSTLAEPSGPVNRRTSVLFRKSKSVSPQKPVRGGEAQAECPQLGARTFLSVVIPRLETLLHTKKRTCNANRGSEGEDELPAKRPDTGLSNGFVLEQQKELNPSRQQEPRRRCASESSISSSGSLLGATSSVPLSFPKCGKGKPALVRRNTVDDKSELIACIETGNFAKAARLAAEVGNSNIWMPANAATVVLEPLKLVWAKCSGYPSYPALIIDPHMPRVGCHHNGVSIPTPPLDVLRIGEQMQYKSEEKLFLVLFFDNKRSWQWLPKSKMVPLGIDKTIDKIKMMEGRTSAIRKAVQIAFSRAMNHMSRVQDEPTSDLSDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLG3</accession>
    <name>A0A3B4DLG3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIH1 domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PIH1D2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568522.1">
        <property type="nucleotide sequence ID" value="XM_017713033.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLG3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024300"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010471.2">
        <property type="protein sequence ID" value="ENSPNAP00000024300.1"/>
        <property type="gene ID" value="ENSPNAG00000008804.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436509"/>
    <dbReference type="CTD" id="120379"/>
    <dbReference type="GeneTree" id="ENSGT00510000048581"/>
    <dbReference type="OMA" id="SCLCTEI"/>
    <dbReference type="OrthoDB" id="545063at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF6">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="3">
        <location>
            <begin position="51"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="4">
        <location>
            <begin position="253"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="321" mass="35735" checksum="D86F4F921F951D90" modified="2018-12-05" version="1">MDRGPGGQSAAVLQQVDQFWSILDEMSLNDPGAYKMFIDRQLREGAEYLSPPEPHCCLRASLQKPEEGILYVNLCGWKRVPAPTSSTQPLPVCGGRLETVSEENENYYIVDVALNPEVLQRAEGDTQEKEQIHLLALSFIQQHHNLNLSKHFTVTKGKLKGSVQNVKSRLMAPPQSKFSSPNPQTESATSLIQQISSLRMEETKEDSTIHLSVGQEEGRAQPGLIEVICSTESAQPQLPKHQFTVCAETGDSSRSLKLSVELPGVRSVSQCELSITQDDILLKVEEMYYLHLQFPERVNEESCCASYNKKKHVLTVTASVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLN7</accession>
    <name>A0A3B4DLN7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037400149.1">
        <property type="nucleotide sequence ID" value="XM_037544252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLN7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024026"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011220.2">
        <property type="protein sequence ID" value="ENSPNAP00000024026.2"/>
        <property type="gene ID" value="ENSPNAG00000001043.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430290"/>
    <dbReference type="GeneTree" id="ENSGT00910000144592"/>
    <dbReference type="OMA" id="MFLVEVY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000448">
        <property type="entry name" value="RE1-silencing transcription factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001156">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF63">
        <property type="entry name" value="SI:DKEY-154P10.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="349"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="575"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="603"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="631"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="650"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="665"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024026.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="680" mass="77540" checksum="37AD84086FC8B596" modified="2024-11-27" version="2">MSHFTPNHVHIMGGIKDAYQDVQPVTLCLAEESVTSHLYSHSAKGIEAHLSTLVEAFLIEVFRCRVCQFTSSQKARISSHVSERHSSPSPCTHLSCLEKDDEKSLTVGMRVDEEVELDQSGSPYDLHSDSKNSEDQMDMDRMSFLMYGMLQNISPPPCDIGLSSNSDGNLHVAQTCEVSTLFEEDRHGEGSEEEPVFQLEDTRDGLPGPLSAGMACTEDQDEEMAQSAHLMTLGLCRISSTKCPSQPAPLVSRLALTAGGQDALDASADDKQPLLTTAELQKQAEEDGGLSCVLCQVNMVSHSLLEVHLKCHDGEQGFKCPRCGWATVEWVNMERHWRGHGRRRSSKPHKCAVCHRTFRRADSRDAHEKRHNRHHRTRPKSSPLAQCSLCLEWCHSDREWEIHQRCHFQGSFKCLYCDFREPSWKKIHKHILNRHTQDDPDPDHQNSYHVGQLKLDTQPSSTYPKCLKGVPPESWCQVRKTRVKHKIVGRKKSEEEKKDGVRLTEKIREGGKVSKRKEFCCTLCNRKFSTKLTMRRHMGIHQGDKPFECPHCHYCTRLKASLIQHLRIHTGEKPYKCPQCSYASIDRSSLRRHLRTHTQEKPYHCQYCPYSSIQKKSLDLHSRRHHTGESFPCSLCQYSTPDRQLLLRHMRKHHSSEQSPALGLRSAPRSSSSNRPRTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMH6</accession>
    <accession>A0A3B4BWU6</accession>
    <name>A0A3B4DMH6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">E3 ubiquitin-protein ligase Mdm2</fullName>
            <ecNumber evidence="4 13">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MDM2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
            <location evidence="13">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
            <location evidence="13">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the MDM2/MDM4 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4 13"/>
    <dbReference type="RefSeq" id="XP_017563091.1">
        <property type="nucleotide sequence ID" value="XM_017707602.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017563092.1">
        <property type="nucleotide sequence ID" value="XM_017707603.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017563093.1">
        <property type="nucleotide sequence ID" value="XM_017707604.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMH6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009554.2">
        <property type="protein sequence ID" value="ENSPNAP00000024665.2"/>
        <property type="gene ID" value="ENSPNAG00000009335.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433188"/>
    <dbReference type="CTD" id="4193"/>
    <dbReference type="GeneTree" id="ENSGT00530000063539"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:identical protein binding"/>
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        <property type="term" value="F:p53 binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of apoptotic process"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase Mdm2"/>
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        <property type="entry name" value="Znf_RanBP2"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46858">
        <property type="entry name" value="OS05G0521000 PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-C3HC4_3"/>
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        <property type="entry name" value="RING/U-box"/>
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        <property type="entry name" value="ZF_RING_2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
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            <begin position="436"/>
            <end position="477"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="489" mass="53932" checksum="07210B98F9F57183" modified="2024-11-27" version="2">MATESCLSSCQINKIDNEKLVRPKVQLQTLLKDAGADKDVFTMKEVMFYLGKYIMSKELYDKQQQHIVHCGNDALGAVLGVASFSVKEPRALFAMISKNLIAVKNPESQSTFSEPRSRTEPDRGPEGINSDSSTSVQQQQQQQRRRRRSSDPSSFSVEDESSEPRKRHKSDSFSLTFDDSLSWCVIGGLRRERESSDSSDSCSNSDVGVSHSENSDESEENSDSDNFSVEFEVESIDSDAYSENDDDSVPGEDEVYEVTIFAEDEDSFDEDPEITEEDYWRCSECDKLNPPLPRHCKSCWKVRPDWLPEINSSLEHPSNDTETSAAADGTCLSTNLSSASECKPLPSKPSSPTHDPDEGMDVPDGKCSRSPATSRDELTAANAAATSISDSQGSSLSSSQPSTSSLQVAHSQEETPELERFNSLEASLPTTCLEPCVICQSRPKNGCIVHGRTGHLMACYTCAKKLKNRNKLCPVCREPIQSVVLTYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMP2</accession>
    <name>A0A3B4DMP2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ZBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">FBXO5</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017558149.1">
        <property type="nucleotide sequence ID" value="XM_017702660.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMP2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024376"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010287.2">
        <property type="protein sequence ID" value="ENSPNAP00000024376.1"/>
        <property type="gene ID" value="ENSPNAG00000008894.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430280"/>
    <dbReference type="CTD" id="26271"/>
    <dbReference type="GeneTree" id="ENSGT00530000063692"/>
    <dbReference type="OMA" id="VVLSCMQ"/>
    <dbReference type="OrthoDB" id="9984940at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045835">
        <property type="term" value="P:negative regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20348">
        <property type="entry name" value="BRcat_RBR_EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047147">
        <property type="entry name" value="FBX5_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044064">
        <property type="entry name" value="ZF_ZBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15493:SF8">
        <property type="entry name" value="F-BOX ONLY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15493">
        <property type="entry name" value="F-BOX ONLY PROTEIN 5 AND 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51872">
        <property type="entry name" value="ZF_ZBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZBR-type" evidence="15">
        <location>
            <begin position="307"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="6"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="381" mass="41732" checksum="A1B438F526CD3CD7" modified="2018-12-05" version="1">MKCFALTRGSAGSLHTEKDKVQSPVELKGQTAPTSATGSGQVTLAVSCPTEEHLKGPHNKENCREKRCSADFPSDDVVNLTSSSLAEDSGYLSLHNSQIEHVDSDTNVAESSERCAEQRLSTASSDSSSSCLPVLKFQEEVCKALANGYKKSQSYDWTVINRIAELHGLQNVIGGKMGLEHVDILCGLLKKDMKHILTRILGLLGESDLISCKRVSKTWRKIICQDDRALQRCRNAEKVLRDSGRSTGSLSRDFCLSRVVLSCLQTRVTSSTPVHKPKKTLTIVGMPNTSRFAGFHEATKSLKSHESLRSCRLCSSPARFDSAMQRAVCMRISCAFEFCSQCQSAYHGSAPCQRGMIRASPRSQTVPVAGSARSKRSIRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMW1</accession>
    <name>A0A3B4DMW1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017581076.1">
        <property type="nucleotide sequence ID" value="XM_017725587.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017581077.1">
        <property type="nucleotide sequence ID" value="XM_017725588.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMW1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010122.2">
        <property type="protein sequence ID" value="ENSPNAP00000024441.1"/>
        <property type="gene ID" value="ENSPNAG00000001273.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444406"/>
    <dbReference type="GeneTree" id="ENSGT01020000230438"/>
    <dbReference type="OMA" id="DHWPFGI"/>
    <dbReference type="OrthoDB" id="10044919at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14974">
        <property type="entry name" value="7tmA_Anaphylatoxin_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000034">
        <property type="entry name" value="G-protein coupled receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF48">
        <property type="entry name" value="C3A ANAPHYLATOXIN CHEMOTACTIC RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00526">
        <property type="entry name" value="FMETLEUPHER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="48"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="326"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="352" mass="39509" checksum="78BD8183C7480E16" modified="2018-12-05" version="1">MTSNGTLIVVPHANIVGKNDKTTIDIDAIMDNVTIVLYTITVLLGTTGNAVVIWMAGFSLKPNVTNVWLVNLAVADLIFCLTRVTSLIKKLFYDYWPFGIFLCKFNGFFKYGNMFCSVFLLAVISIDRALCVWRPVFTRRRRTLLAARLISLGVWIVAVIFSAPYYVYRQVYLGKNNLSKCSLEVKESTEGDNTTKLALYSIRFTCGFLLPFLVILTCYALAGLGIRRTHISRKSRPLRILVSLVCAFFLCWGPYHCLLLAKMVNSKSQAVKVGLPIAKGVAYFNSCVNPLLYFCMGLDIRQRFSQSLSGVYRRALAEEWDGQTLQSQERSVDESSSHNTQVKVTGMTTAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQC2</accession>
    <name>A0A3B4DQC2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Serine/threonine-protein kinase receptor</fullName>
            <ecNumber evidence="21">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:18674"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:44881"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="21">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Membrane</location>
            <topology evidence="21">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="21"/>
    <dbReference type="RefSeq" id="XP_017539964.1">
        <property type="nucleotide sequence ID" value="XM_017684475.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQC2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025149"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008342.2">
        <property type="protein sequence ID" value="ENSPNAP00000025149.2"/>
        <property type="gene ID" value="ENSPNAG00000010092.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412453"/>
    <dbReference type="CTD" id="553359"/>
    <dbReference type="GeneTree" id="ENSGT00940000157233"/>
    <dbReference type="OMA" id="TDKRSHC"/>
    <dbReference type="OrthoDB" id="547665at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048179">
        <property type="term" value="C:activin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048185">
        <property type="term" value="F:activin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017002">
        <property type="term" value="F:activin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030902">
        <property type="term" value="P:hindbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23631">
        <property type="entry name" value="TFP_LU_ECD_ACVR2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000099">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000094">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017194">
        <property type="entry name" value="Transform_growth_fac-b_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF64">
        <property type="entry name" value="ACTIVIN RECEPTOR TYPE-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037393">
        <property type="entry name" value="TGFRII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00653">
        <property type="entry name" value="ACTIVIN2R"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="21" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043904642" description="Serine/threonine-protein kinase receptor" evidence="22">
        <location>
            <begin position="24"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="214"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="105"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR037393-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR037393-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR037393-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025149.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="535" mass="59988" checksum="1570762DA5F0228B" modified="2024-11-27" version="2">MGPATKLAFGVFLISCSSGAILGRSETQECVLYNYNPSSENQGNRSIEPCVGDKDKRLHCFATWRNVSGTVAVVKQGCWLDDVNCYDSTECVEKKEDPEVFFCCCEGNMCNEKFYHNPDAQAVQSKAPITDRSSGVFKATSNPFTQKPPLFNTLLYSLVPIMGIAAIVLLSFWMYRHHKLAYPPVLVPTQHAFHIMIEDPGPMPPSPILGQKPLQLLEIKARGRFGCVWKAQLLSDYVAVKIFPIQDKQSWQNEYEIYNLPGMRHENILHFMGAEKRGANLDIELWLITAYHEKGSLSDYLKANVVTWNELCHIAQTMARGLAYLHADIPGLRDGHKPAIAHRDVKSKNVLLKANLTACVADFGLALRFEAGKSAGDTHGQVGTRRYMAPEVLEGAINFQRDAFLRIDMYAVGLVLWELASRCTAADGPVDEYMLPFEEEVGQHPTLEDMQEVVVHKKLRPTLRECWQKHPGLSMLCETIEECWDHEAEARLSAGCVEERVVQMHRQTSISGPEEIVTVVTMVTNVDFPPKESSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQH9</accession>
    <name>A0A3B4DQH9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Delta-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">DLL4</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Putative Notch ligand involved in the mediation of Notch signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548077.1">
        <property type="nucleotide sequence ID" value="XM_017692588.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQH9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025204"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008196.2">
        <property type="protein sequence ID" value="ENSPNAP00000025204.1"/>
        <property type="gene ID" value="ENSPNAG00000010188.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424520"/>
    <dbReference type="CTD" id="54567"/>
    <dbReference type="GeneTree" id="ENSGT00940000167177"/>
    <dbReference type="OMA" id="ICLAGCT"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046331">
        <property type="term" value="P:lateral inhibition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035282">
        <property type="term" value="P:segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000064">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000117">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.140:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3510:FF:000003">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000434">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01414">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="8"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 17" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
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    <keyword evidence="3 15 17" id="KW-0245">EGF-like domain</keyword>
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    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
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    <keyword evidence="11 17 18" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017470944" description="Delta-like protein" evidence="19">
        <location>
            <begin position="22"/>
            <end position="673"/>
        </location>
    </feature>
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        <location>
            <begin position="517"/>
            <end position="540"/>
        </location>
    </feature>
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        <location>
            <begin position="160"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="271"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="311"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="349"/>
            <end position="387"/>
        </location>
    </feature>
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        <location>
            <begin position="389"/>
            <end position="425"/>
        </location>
    </feature>
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        <location>
            <begin position="427"/>
            <end position="463"/>
        </location>
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        <location>
            <begin position="465"/>
            <end position="502"/>
        </location>
    </feature>
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        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
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        </location>
    </feature>
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            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
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            <begin position="337"/>
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        </location>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51051"/>
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            <dbReference type="Proteomes" id="UP001501920"/>
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    <sequence length="673" mass="74161" checksum="BF35FFDF75FC83FF" modified="2018-12-05" version="1">MAARLTFLLAMLSSLITQMFGSGVFELDLHEFKNFKGLLANGEPCRPECRTFFRVCLKNYQAVVSPGDCIFGSTVTPVLGTNSFRVGGEPLSTPIRLPFSFGWPGSFSLIIEAWYSPVDSNNPDLMISSFAVQRQLEVGAEWSQDVQSGQQTELRYSYRFICNENYYGDSCSKICTPRDDRFGHYTCNPDGQLSCLPGWKGEYCEEPICLKGCSEANGNCSKPGECVCREGWQGTFCTECKKHPVCKHGTCQQPWQCNCKEGWGGLFCDQDLNFCTHHKPCKNGATCMNTGQGSYTCTCMPGYTGVNCEMEVRQCDSKPCRNRGHCVELDNSYKCNCTPGFEGTHCEHSLLTCADVPCFHGGKCHEKDNGRSYSCDCPRGYTGLNCEKRLDKCTTLPCANGGACVVLGGVSMCRCHTGFTGQRCEININECTSNPCANGGTCHDRISDYVCSCPPGYGGRNCNQLTGGCPAQFCLNGGSCATVPGGSPACFCPSSFTGPHCEYFAVTPTVETEGFQWAAVSLAVGLVALLVLLCMVAIALRHIHRQANDERTHADAMNNLSDTQRDNLIPTSQLKNTNKKVGLEVDCGPEKSNYIHKNYHLDYNSKDFKETPLQEDKSHNYEKCLEDKIPLSRMYSEKPECRISTICSPRDSMYQSVFVIAEERSECVIATEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DSC5</accession>
    <name>A0A3B4DSC5_PYGNA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DSC5"/>
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        <property type="gene ID" value="ENSPNAG00000011280.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026006.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="145" mass="16559" checksum="AE5106A02195540E" modified="2024-11-27" version="2">MTTFSMKGSSMGSGRLSLLMASAEMEKRRAVRKTALESAPTTSTLARPSPTHSDTMSDSMLKESETSAMEFPNTTVITSMKIRRQVLPVYFILLVCPFLKDTHQRKLSFSNFFFFKHHIFHTFPYDAVVGTRHCVFIFLNFQFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DSS4</accession>
    <name>A0A3B4DSS4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Neuropilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the neuropilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DSS4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006148.2">
        <property type="protein sequence ID" value="ENSPNAP00000026019.2"/>
        <property type="gene ID" value="ENSPNAG00000001661.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155270"/>
    <dbReference type="OMA" id="XYDFIEI"/>
    <dbReference type="OrthoDB" id="6155811at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005021">
        <property type="term" value="F:vascular endothelial growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000013">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000010">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014648">
        <property type="entry name" value="Neuropilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022579">
        <property type="entry name" value="Neuropilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF2">
        <property type="entry name" value="NEUROPILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11980">
        <property type="entry name" value="DUF3481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036960">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 17" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 17" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14 19 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="13 17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043601929" description="Neuropilin" evidence="22">
        <location>
            <begin position="23"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="859"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="23">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="23">
        <location>
            <begin position="147"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="24">
        <location>
            <begin position="275"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="24">
        <location>
            <begin position="432"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="25">
        <location>
            <begin position="643"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="19 20">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="275"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="432"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR036960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR036960-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR036960-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026019.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="925" mass="102744" checksum="E51FE0B510D980AF" modified="2024-11-27" version="2">MDLLSRLSGCLLVLCLSSLVRGDEEPCGGHRDASDAGYITTPGYPQEYPPHLDCRWVITAPEPSQRIVLNFNPHFELEKLDCRYDFIEIRDGNSDSADLLGKHCSNIAPAAIISSGPVLLIRFVSDYAHQGAGFSLRYEIHKTGSDCSRNFTSPSGVIESPGFPDKYPHNLECTFIIIVPPHMEVTLTFLTFDLENDPLQGSEGECKYDWLEVWDGLPQVGPLIGRHCGTKIPPELKSSTGILSLTFHTDMAVAKDGFSARYNMTLKEVSDNFHCSNAFGMESGHITDDQITASSSFSDGHWAPRQARLNYNENAWTPAEDSSKEYIQVDLHFLKVLTGIATQGAISKETHKSYFVTTFKLEVSTNGEDWMVYRHIKNHKVFLANTDPSEVVLNRIPQPVLARFVRIRPQTWKNGIALRFELYGCQITDAPCSEMQGMLSGLLPDSQITASSMRDIHGSTGAARLVASRSGWFPSPTQPVAGEEWLQVDLGVPKTVRGIITQGARGVDGSNSAESRAFVRKYRLAHSMNGKDWSFFLDPKTGLPKIFEGNTHYDTPEIRRFDEVVTQFIRVYPERWSPAGIGMRLEILGCDLPESTTIPDTATPTLPQQEETSTAHRAAVMSVTYLVSLSLSPAPTTLSDLGGLCDFEQNMCGWTHDPTADLNWALHSPSSSNMDLQLGADDYGSFLYLEANPKTEHERARLLSPPVGPERGPLCLLFLYQLTGEAQGTLRVLIQDHNQDEALLWTLKGEQGHSWKEGRTILPRSPKEYKVVIEGFFEHGSRGHIWIDNIHMSTTTELEQCTQPFPAFPPEMTGELFCHILVQMSVIFLSWTTPGPSRGPPVTRVSEKDNAWLLTLDPILVTIIVMSSLGVLLGAVCAGLLLYCTCSYNGLSSRSSTTLENYNFELYDGIKHKVKINHQRCCSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUB0</accession>
    <name>A0A3B4DUB0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUB0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027050"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003569.2">
        <property type="protein sequence ID" value="ENSPNAP00000027050.2"/>
        <property type="gene ID" value="ENSPNAG00000012466.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441919"/>
    <dbReference type="CTD" id="403133"/>
    <dbReference type="GeneTree" id="ENSGT00940000164422"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000001">
        <property type="entry name" value="Toll-like receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000046">
        <property type="entry name" value="Toll-like receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017241">
        <property type="entry name" value="Toll-like_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF26">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037595">
        <property type="entry name" value="Toll-like_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="14" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="606"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="18">
        <location>
            <begin position="659"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="838"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037595-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027050.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="877" mass="100760" checksum="7E94926175AA3BC4" modified="2024-11-27" version="2">MADVRIFTFLSLVFGFQASFFILPVRSELSCRIFADQRTANCQGLRLQRVPVEQLPSSLEVVDLSYNIIHVIRNMDFVGLPLLRVLLLQFNNISVIEDKAFQENLLLEDVNIFNNSLTVIPSKALAPLSRLKNLEMSNNLYSEATLDDAFLNFRKLKVLSMGGPVVGILKKGDLDVLQNVSLEKFAIKTGSSLDVYEPGYLKNIHTNNMWLDMAIDNRTYVLPMILKDLANKTFVVLRFRNLFEFKYYTDKEDIFYGLKYINSQQLIFYRGKFNEELLRMALINLQKSPIKNLGLLFIDFARSQIFVNSQQASSVTNLVLERLYLSDISNPDILRFDWRFTWLNKIRHLHIHNVNFNTVPCDAWEEMRGVEILDISKNQLKDSYLFNQQCNYRETMPSLHTFNLSNNQLTSFGSFAALAGEFKHLQTIDISYNKIGSEGNNICSWKQNITTVIADHNTLVIDSFKCLPTTVLYLDLSYCNLDQLDMDYFRKAIGLKEVYLSGNKIKFIPSGWRSPNLKSLALDGNSFGLISMSSFKGMPNLARLKAGNNPYHCTCDLHTFIQETTVKGKVQITDWPENYKCYYPADLLNTMVSQYFPGHVACDIRLVIVISVVTTAAVVLAIMLLCYVFSIPWYAKATYQIIRAKYRAHKEGAGLSMDYTFHAFISYSHSDADWVRDKLLPCLENSRPPYRICIHERDFMPGKWIIDNIIENIENSQKVIFVLSRHFVNSEWCNYELYFAQQRAIGKTLRDVILVVKEPIDPKSLPSKYCKLKKMLNTKTYLEWPEQPKQQAFFWAQLRSVLGKPNLIKEHSVTEHRRASRLSRISVIELPVENQGSVDVQPKIDTDPGAEVNPQTIGETGNLPENKDLKIPLKQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVU7</accession>
    <name>A0A3B4DVU7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LIM and SH3 domain protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LASP1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DVU7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027191"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003228.2">
        <property type="protein sequence ID" value="ENSPNAP00000027191.2"/>
        <property type="gene ID" value="ENSPNAG00000012620.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154775"/>
    <dbReference type="OMA" id="TPVYHHQ"/>
    <dbReference type="OrthoDB" id="191061at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09447">
        <property type="entry name" value="LIM_LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11934">
        <property type="entry name" value="SH3_Lasp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000087">
        <property type="entry name" value="LIM zinc-binding domain-containing Nebulette"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000007">
        <property type="entry name" value="nebulin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035630">
        <property type="entry name" value="Lasp1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051759">
        <property type="entry name" value="LIM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000900">
        <property type="entry name" value="Nebulin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218">
        <property type="entry name" value="LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218:SF2">
        <property type="entry name" value="LIM AND SH3 DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00880">
        <property type="entry name" value="Nebulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00227">
        <property type="entry name" value="NEBU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51216">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0440">LIM domain</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 19" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="11 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="22">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="21">
        <location>
            <begin position="190"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027191.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="249" mass="28471" checksum="CF6708A95B9CE6A7" modified="2024-11-27" version="2">MNPLCSRCNRVVYPTEKVNCLDKFWHKGCFSCEVCKMTLNMKNYKGFEKRPYCNAHYPKTSFTSVADTPENLRLKQQSKMQSQVLYKEEFEKNKGKGFSVVADTPEMQRIKKTQDQISNIKYHEEFEKSRMGGEAPPHHPPQQQNTTPGISTTPTASQNYHYEPEPVRQAAAAPPPSSGVMYYSCSLCVVFQKRYRAVYDYTAADEDEVSFVDGDLIVDVQPIDEGWMYGRVERTGQQGMLPANYVEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZB9</accession>
    <name>A0A3B4DZB9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein zwilch</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZWILCH</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RZZ complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Chromosome</location>
            <location evidence="1 9">Centromere</location>
            <location evidence="1 9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ZWILCH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547182.1">
        <property type="nucleotide sequence ID" value="XM_017691693.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017547191.1">
        <property type="nucleotide sequence ID" value="XM_017691702.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZB9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015253.2">
        <property type="protein sequence ID" value="ENSPNAP00000028770.1"/>
        <property type="gene ID" value="ENSPNAG00000014620.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423982"/>
    <dbReference type="CTD" id="55055"/>
    <dbReference type="GeneTree" id="ENSGT00390000013696"/>
    <dbReference type="OMA" id="PARTTWF"/>
    <dbReference type="OrthoDB" id="5556307at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018630">
        <property type="entry name" value="Zwilch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995:SF1">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09817">
        <property type="entry name" value="Zwilch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="583" mass="66140" checksum="262C5E8613B74F49" modified="2018-12-05" version="1">MAGSVVSEAKRFIHFLRLCQDDDKDSWAYQENIKISKHDDEPLLSIIEGNQPFFICEKMPPEREPEGAESAEETSLNEEVSVKLEHLGIGPLPLSINTARKLLSWYTMSQNPNMAQLEGHVLHPLWVRCDMSDPAGTSWLGAETVYVGGKATAVKLYTVRSKGATADETSFMTMDQLKQEHQNRHHSSALVTKGWAEYNLFCLVKEESLVIDSQSRVTATFRWTNVEKMLESPPLSSVVTLQIKVTVGDIRSPMFQKFRELEFLLTLAEGLRTGEIEWLDLPETRSTVDLTRELIEGLENVANAVPGQNLKGSENQKSKTDSVLGFSSMLMERGDLDFTEQLWEKMRKSVTSYQDITDSLKLVIKAVRFGQIKPWVHRDSSSSLSRLIVQSYQQQVDTVPLTGLTPAIMLLELGLDKMRKDYINYLVGKELTTLNHLSYYLSTQVDLQEQVVRLKKLHHLLEILGTCCSFLSLPHERLFFFTQSCLQYYKTAPYNEEHVFEMQIKPALISHFYHTEQPTSWAVEVSSGQGSREVKTSWHLSDKPLVDHVPFDSGLPLEVSVSAESEKTAYFRTLVCCSLANFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E032</accession>
    <name>A0A3B4E032_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha and gamma adaptin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">AAGAB</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017547294.1">
        <property type="nucleotide sequence ID" value="XM_017691805.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E032"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015036.2">
        <property type="protein sequence ID" value="ENSPNAP00000028671.2"/>
        <property type="gene ID" value="ENSPNAG00000014473.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424045"/>
    <dbReference type="CTD" id="79719"/>
    <dbReference type="GeneTree" id="ENSGT00390000007218"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019341">
        <property type="entry name" value="Alpha/Gamma-adaptin-bd_p34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14659">
        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14659:SF1">
        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10199">
        <property type="entry name" value="Adaptin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028671.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="35613" checksum="D9FD97974D662440" modified="2024-11-27" version="2">MSTDGEDEKTALPCVVITSCDSGFRGDELVRQILCTSSLPEPAKREDVIWYPWTINNKYYTADVGLCVVPSTFNMNSEVAQSMQAFIVYFDSKTKDGLNGVTPWLSVVDDLAPEVLILVCDRVCENGVSRHEAQQWCLAHAFELVELNPQELPDEDDDFPESTGVKRIVQALNANVWSSVEMKDEHNQGFGLMSSLVASRHNNPRPMPESQPFQSQSNTVDEKTEGQKTESCHQSTEAVDSLVDPMTDMDIQELANLTAGDADVENFERLFTKLKEMKDKASSLPHEQRKLHAEKVAKAFWMAIGGDQDEIDGLSSGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3T5</accession>
    <name>A0A3B4E3T5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3T5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036644.2">
        <property type="protein sequence ID" value="ENSPNAP00000031277.2"/>
        <property type="gene ID" value="ENSPNAG00000003531.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183079"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16175">
        <property type="entry name" value="EFh_MICU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF10">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="423"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031277.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="490" mass="56440" checksum="AAA13185272542DA" modified="2024-11-27" version="2">MAALRGARALWSRVTGSASACGGRLGGRLRAGACLGAAAGGALYFWYQWGARGATEPRHRGALSLLPALRARGHDKEEEEEEARPLNFKDRDVSMSSHEYRFHLFSSVEYEGQLFMTPQNFIESVTMSQPRNKKPWRSLTKQELEKILVDTPPVWKGTSKLFRNLRERGIISYTEYLFLLCILTKPHAGFKIAFNMFDADGNQMVDKQEFLVLQEIFRKKNEKRGRKGDSEKSAQLSMQLYGYQVAPVNSAMKKDSQEEFVARSYWDMLRRGASQALFSDLAERTDDSMTIDTTLLVHFFGKKGKAELTFDDFYRFMDNLQTEVLEIEFLTYSKGMTTISEEDFARILLRYTNVEDIRSYLENVRQSIPDEKGITFDEFRSFFQFLNNLEDFAIAMQMYNFASRSIGQDEFARAVYVATGLKLTRHLVNTIFKIFDVDHDDQLSYKEFIGIMKDRLHRGARVNGGSFLACVRSEAGQHVHQLWKQFRKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E436</accession>
    <name>A0A3B4E436_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:50708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57444"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:90680"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:50709"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:16834"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer. Interacts with MIEN1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E436"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029999"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017626.2">
        <property type="protein sequence ID" value="ENSPNAP00000029999.1"/>
        <property type="gene ID" value="ENSPNAG00000016445.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165239"/>
    <dbReference type="OMA" id="QENTRND"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010269">
        <property type="term" value="P:response to selenium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000315">
        <property type="entry name" value="Glutathione peroxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF41">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="142" mass="16281" checksum="8A127DE3D41F6E33" modified="2018-12-05" version="1">MNELHAKYSDKGLVVLGVPCNQFGHQENGKNEEILNCLKYVRPGNGFEPRFSLLEKVDVNGKDAHPLFVYLKENLPYPSDNSMALMSDPKFIIWSPVCRNDVAWNFEKFLVGPDGEPFKRYSRTFLTSDIEADIKELLKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E544</accession>
    <name>A0A3B4E544_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipid scramblase CLPTM1L</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cisplatin resistance-related protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cleft lip and palate transmembrane protein 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CLPTM1L</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Scramblase that mediates the translocation of glucosaminylphosphatidylinositol (alpha-D-GlcN-(1-6)-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol, GlcN-PI) across the endoplasmic reticulum (ER) membrane, from the cytosolic leaflet to the luminal leaflet of the ER membrane, where it participates in the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a lipid glycoconjugate involved in post-translational modification of proteins. Can also translocate 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) (phosphatidylinositol or PI), as well as several other phospholipids (1,2-diacyl-sn-glycero-3-phosphocholine, 1,2-diacyl-sn-glycero-3-phosphoethanolamine), and N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI) in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = 6-(alpha-D-glucosaminyl)-(1-octadecanoyl,2-(9Z)-octadecenoyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71495"/>
            <dbReference type="ChEBI" id="CHEBI:190691"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(in) = a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol(out)</text>
            <dbReference type="Rhea" id="RHEA:71491"/>
            <dbReference type="ChEBI" id="CHEBI:57997"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E544"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030808"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019198.2">
        <property type="protein sequence ID" value="ENSPNAP00000030808.2"/>
        <property type="gene ID" value="ENSPNAG00000017807.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063461"/>
    <dbReference type="OMA" id="TTMWRAF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030808.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="539" mass="62655" checksum="266A1E7F544F8490" modified="2024-11-27" version="2">LWSLCTTSFTTLIVGVFIVYVLHTCWVMYGIVYTKPCEYPRSDKCISPYLASNPPLQLSIYTALRPNGEGGHSLIHREEAFDVHTRFERLINVSLPKKTRKNGTLYAMVFVHQPGMSPWQDPRQVHLVTQLTTYMLPKPPEVSLITGEDEPQQQQNPNMGADDKLDRPVSHWRSRLTLNVVAENFHFDREALPSDVHRYMKVFQSGRKMVYLPLLFIDQLSNRVKDLVINSSTTELPLTISYDTIALGKLRFWIHMQDAVYSLQQFGFTEKDADEIKGIFVDTNLYFLALTFFVAAFHLLFDFLAFKNDISFWKQKKSMVGMSSKAVLWRCFSTIVIFLYLLDEQTSLLVLIPAGIGSLIEVWKVKKAFKIYFMWKGGKPTFLFGKSDESEKRTEEYDSLAMKYLSYLLYPLCVGGAVYALVFVKYKSWYSWLINSLVNGVYAFGFLFMLPQLFVNYKLKSVAHLPWKAFMYKAFNTFIDDVFAFIITMPTSHRLACFRDDAVFLIYLYQRWLYPVDKTRVNEYGVSYDEDPKKKIHKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E6S5</accession>
    <name>A0A3B4E6S5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methylmalonyl-CoA mutase, mitochondrial</fullName>
            <ecNumber evidence="4">5.4.99.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methylmalonyl-CoA isomerase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-methylmalonyl-CoA = succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:22888"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57326"/>
            <dbReference type="EC" id="5.4.99.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:22889"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Interacts (the apoenzyme form) with MMAA; the interaction is GTP dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylmalonyl-CoA mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017539295.1">
        <property type="nucleotide sequence ID" value="XM_017683806.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017539300.1">
        <property type="nucleotide sequence ID" value="XM_017683811.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393932.1">
        <property type="nucleotide sequence ID" value="XM_037538035.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393933.1">
        <property type="nucleotide sequence ID" value="XM_037538036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E6S5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031378"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036694.2">
        <property type="protein sequence ID" value="ENSPNAP00000031378.1"/>
        <property type="gene ID" value="ENSPNAG00000018712.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411975"/>
    <dbReference type="CTD" id="4594"/>
    <dbReference type="GeneTree" id="ENSGT00390000011892"/>
    <dbReference type="OMA" id="IQEETHI"/>
    <dbReference type="OrthoDB" id="198977at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019678">
        <property type="term" value="P:propionate metabolic process, methylmalonyl pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02071">
        <property type="entry name" value="MM_CoA_mut_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03679">
        <property type="entry name" value="MM_CoA_mutase_alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.240:FF:000002">
        <property type="entry name" value="Methylmalonyl-CoA mutase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.280:FF:000002">
        <property type="entry name" value="Methylmalonyl-CoA mutase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.240">
        <property type="entry name" value="Methylmalonyl-CoA mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058549">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006944">
        <property type="entry name" value="PRK09426.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF4">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="Cobalamin (vitamin B12)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00544">
        <property type="entry name" value="METMALONYL_COA_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="11" id="KW-0170">Cobalt</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="B12-binding" evidence="16">
        <location>
            <begin position="623"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="759" mass="83878" checksum="BFA5ED4F910930DB" modified="2018-12-05" version="1">MLTAGKVCSSLAARRLLGASGSVCPALDQQRRSRLFHTATPHRDEVQLHEAWASLAKKQLKGKNPEDLIWRTPEGISIKPVYTQTDTAKVADELPGVFPYTRGPYPTMYTFRPWTIRQYAGFSTVEESNKFYRDNIKAGQQGLSVAFDLPTHRGYDSDNPRVHGDVGMAGVAIDTVEDMKMLFDGIPLERMSVSMTMNGAVIPILAMFIVTGEEQGLDKAMLTGTIQNDILKEFMVRNTYIFPPEPSMHAIADIFAYTSKHMPKFNSISISGYHLQEAGADAILELAYTIANGLEYCRTGIKAGLTIDEFAPRLSFFWGIGMNFYMEIAKMRAARRLWATLIKEKFQPKNSKSLLLRTHCQTSGWSLTEQDPYNNVIRTVIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMEALTNDVYSAALKFITEIEEMGGMARAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYRLENEETVEVLAIDNTIVRNKQIEKLHKVRKARDADAAKRCLAAIEQCARTRDGNLLQLAVEAARARCSVGEITDAMKSVFGEHKASTRMVSGAYRSEFGEHEEIALAHGRVVQFKKHEGRNPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPLEVAQQAVDADVHCVGVSTLAAGHKTLVPELIKELRNMNRPDILVICGGVIPPQDYEFLYQSGVCCIFGPGTRIPQAAVEVIDNIEKSLEKNRQAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7V2</accession>
    <name>A0A3B4E7V2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567206.1">
        <property type="nucleotide sequence ID" value="XM_017711717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7V2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031376"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020287.2">
        <property type="protein sequence ID" value="ENSPNAP00000031376.1"/>
        <property type="gene ID" value="ENSPNAG00000018689.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435710"/>
    <dbReference type="CTD" id="556658"/>
    <dbReference type="GeneTree" id="ENSGT00940000158635"/>
    <dbReference type="OMA" id="CYNMFVW"/>
    <dbReference type="OrthoDB" id="414826at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000003">
        <property type="entry name" value="Peptidase inhibitor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017405908" description="SCP domain-containing protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="8">
        <location>
            <begin position="64"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="257" mass="29305" checksum="F4C932C8595237C1" modified="2018-12-05" version="1">MRNSDFFLIDLMLLCIATETSAWTLRNATDSLSGANGTVRTSSAEEHLDASLPRSRRKRYISQSDMIAILDYHNKVRASVFPPAANMEYMVWDEGLARSAEAWAATCIWDHGPPYLLRYLGQNLSVRTGNYRSILQLVKPWYDEVKDYVFPYPRDCNPRCPMRCYGPMCTHYTQMVWATSNRVGCAIQVCYNMVVWGAVWRQATYLVCNYSPKGNWIGEAPYRVGVPCSACPPSYGGSCSNNMCFPAMNSNFMYWFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7X9</accession>
    <name>A0A3B4E7X9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="8">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017580642.1">
        <property type="nucleotide sequence ID" value="XM_017725153.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393531.1">
        <property type="nucleotide sequence ID" value="XM_037537634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7X9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031359"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020271.2">
        <property type="protein sequence ID" value="ENSPNAP00000031359.1"/>
        <property type="gene ID" value="ENSPNAG00000018661.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444159"/>
    <dbReference type="CTD" id="326020"/>
    <dbReference type="GeneTree" id="ENSGT00940000165510"/>
    <dbReference type="OMA" id="RLWCMER"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000072">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002374">
        <property type="entry name" value="cGMP_dep_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF118">
        <property type="entry name" value="PROTEIN KINASE CGMP-DEPENDENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000559">
        <property type="entry name" value="cGMP-dep_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0140">cGMP</keyword>
    <keyword evidence="7 8" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="14">
        <location>
            <begin position="108"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="14">
        <location>
            <begin position="226"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="372"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="378"/>
            <end position="386"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000559-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000559-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="679" mass="77804" checksum="AE9CF614617DD8F5" modified="2018-12-05" version="1">METKLKELRQKLEKQCQVNQELQNHNQDLEKRLKEKERLLEELQSRLDELEGHSPRESNDGVQEVRQSRTAVMALEPIPEDLDIQTTKVKKTAGERAQIIKAMGKNDFLSRLDEEQISMMVELLTSLDRCPGDKIIKEGTEGDSMYIVAAGELKVTQSGRDLRTLTSGDVFGELAILYNCKRTASVEAVTAVRLWCIDRQTYRSIMTSKSKKKREQLMGFLKTARTLKALNEEQLSNIIDSMEEVKFQDTEVIVREGAEGDTFYIILKGEVLVTKKVNGQQKVIRKMGQGEHFGELALIRAILRTATCTAVGEVTCFSIDKEVFEETIPIESLELHDDSDLENTVTSEKERFESSLKLKDLIPILYQEGRYQGEPVTLGLGGFGRVELVTTLHHGKYFAMKKVSKKLIVEKRQGPHVIFEKRILQDIESDFIVRLHAAFKDTRYIYMVMEFCSGGEIWTKLKEVGRFDENIAVFVSACVVEAYAYLHKKNIMYRDLKPENLMLDSKGYVKLVDFGFAKELKRGQKTYSFCGTPEYMAPEIIQNHGHDFAADFWSLGVLVFELLVGSPPFSSSEPQKIYAKILDGVMKFPPYMGEGARSLINKLCRPRPGQRLGNTKNGIKDVRHHRWFNSINWHKLRMGQIDAPTIRLIRKGPCYINFDRFPFDKTQAEEEFSGWDRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAA8</accession>
    <name>A0A3B4EAA8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017537890.1">
        <property type="nucleotide sequence ID" value="XM_017682401.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EAA8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033477"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024418.2">
        <property type="protein sequence ID" value="ENSPNAP00000033477.1"/>
        <property type="gene ID" value="ENSPNAG00000022138.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411040"/>
    <dbReference type="GeneTree" id="ENSGT00500000045140"/>
    <dbReference type="OMA" id="IKNSHAF"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF4">
        <property type="entry name" value="DYNAMIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="7">
        <location>
            <begin position="60"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="450" mass="50275" checksum="78BB1CE5C69F5AB8" modified="2018-12-05" version="1">MSGKVRNRSAANADSVPGSVSCDERILRDCHNMYTQPDSGLITIAQSVGVTLLPPRKKITVMLMGNHSAGKSSFINWYIEEHIQRTGVAIETQGFTFVTSGRKRESLTGNATLHLYPHFKPLQEFKGVSEYLGTEICTSRQKRFSLVTFVDTPGLVDGDMKYPFDVDQAILWLGDLCDLILVFFDPMGQALCKRTLNIVEMLNEKHGDRLRFYLSKADEAGGESDRQRVMMQIVQELCKRPGLNKCGFDMPTIYVPNTNKPSRCVNQIEEVCRTIEKTINQTVQNTLNSLEKDCELISEAVTDTLDNDRQCSVGNRRARCKGCALGLLGFTIPLLMLAALVLGSLSKEMLDMVLGADGTEALTIYLAPVVRAFESVSGEMQLYVCGGLVLLSFILLILSRFSSRTKATLSGKQKRQLQEKLEYVQEVVKSKKKELYEEYLRQSVGDQDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECT1</accession>
    <name>A0A3B4ECT1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C19orf12</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C19orf12 family.</text>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00029457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033041.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="17571" checksum="654AA187159D62C6" modified="2024-11-27" version="2">MVYIFECLSFHLSPSPETEGLLSNMSQRVDDVMKLCCELSANQQVKTAVKHSGKGALTAGGLAFAGGLVGGPLGIAVGGAVGGLLGCWMTSGQFKPLPQVIMEMTTEQQQKLYEDIMAIVGSIDWTDVAQLTTIVLGSASLQQQVTAALLNYIHKELQAEVHYID</sequence>
</entry>
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    <accession>A0A3B4EDJ0</accession>
    <name>A0A3B4EDJ0_PYGNA</name>
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        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A3B4EDJ0"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V-set"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="34"/>
            <end position="148"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033895.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="149" mass="16553" checksum="1AC5B69AB4676ED4" modified="2024-11-27" version="2">MKAPPLSPLFILHSVHHSADHTDNMLPTLCVLLPALTWVSSQKILTQSPSVLTDQGRTVSLDCNIAKDHSYYVSWYKQIPQEAPQFVLRFYHSHSSPDLYGDNFLSSRFTSKASLSIDYQLIISNVEVGDAAVYYCGTWDSSASVGVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EE67</accession>
    <name>A0A3B4EE67_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CARHSP1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSPNAG00000003758.2"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034130.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="167" mass="17989" checksum="DAFAD92B536AB3BF" modified="2024-11-27" version="2">MSSETTSPVQTAPSTTPPLSPSSPISPSTLRLPHCRQRDRSPSPMRGYLIPSPLPTRRNRTCSATARASEGPVFSGVCKCFSRSKGHGFITPSDGGADIFVHISDIEGEYVPVEGDEVSYKICSIPPKHEKIQAVEVTITHLAPGTKHETWFQCCTSSSVGGSELEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EEQ1</accession>
    <name>A0A3B4EEQ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Anthrax toxin receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
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    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton and thereby plays an important role in normal extracellular matrix (ECM) homeostasis. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. May also act as a receptor for PLAU. Upon ligand binding, stimulates the phosphorylation of EGFR and ERK1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with gelatin and type 1 collagen. Interacts with the actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Filopodium membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cell projection</location>
            <location evidence="13">Lamellipodium membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 17">Belongs to the ATR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569819.2">
        <property type="nucleotide sequence ID" value="XM_017714330.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EEQ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034315"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038468.2">
        <property type="protein sequence ID" value="ENSPNAP00000034315.2"/>
        <property type="gene ID" value="ENSPNAG00000023501.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437311"/>
    <dbReference type="CTD" id="563907"/>
    <dbReference type="GeneTree" id="ENSGT00940000157727"/>
    <dbReference type="OMA" id="KNHWDEI"/>
    <dbReference type="OrthoDB" id="10035766at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000017">
        <property type="entry name" value="Anthrax toxin receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017360">
        <property type="entry name" value="Anthrax_toxin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008399">
        <property type="entry name" value="Anthrax_toxin_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008400">
        <property type="entry name" value="Anthrax_toxin_rcpt_extracel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059:SF16">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05586">
        <property type="entry name" value="Ant_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05587">
        <property type="entry name" value="Anth_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038023">
        <property type="entry name" value="Anthrax_toxin_receptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043478643" description="Anthrax toxin receptor" evidence="22">
        <location>
            <begin position="29"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="23">
        <location>
            <begin position="42"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="394"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="465"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="394"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="470"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="494"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="37"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR038023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR038023-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038023-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034315.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="561" mass="61617" checksum="53BE1B97B4DC669C" modified="2024-11-27" version="2">MASFLQRALLLAALSGFLFFCAPSGAHAQDEEDQRSCQGAFDLYFVLDKSGSVKHHWHEIYSFVESLAEKFISPLLRMSFIVFSSRGNTIMKLTEDRERITKGLNMLRSEIPGGDTFMHLGLKEANQQIHQENFGKASVIIALTDGELNEHQLDAAQQEAARARTLGAIVYCVGVKDFNETQLATIADTIEHVFPVTGGFQALRGVIDSIIKKSCIEILAAEPSSVCAGESFQVVVRGNGFLHARKIDQVMCSFKINDTVTLNVKPDGIEDTYLLCPAPVIDEVGKVVYLQVSMNEGLSFITSSVHITTTECFDGTILLITLLVLFLLLALLFLWWFWPLCCTVVIKEPPPPPPPQPESDDEDGLPKKKWPTVDASYYGGRGVGGIKRMEVRWGGKGSTEEGAKLEKPKNAVVKMPEQEYEPFEPKPKKQGNKKATQDRKWYSPIRGKLDAIWALFRRGYDQVSLMRPQPGDHGRCINFTRVKEEAAKAQPIAQGPPPPPPDYRPAPNPVSPPPQCPPPRGPPQAQPAPGPPPAQPAPGPPPARVPPGPPCPPPARPPPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EG47</accession>
    <name>A0A3B4EG47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">D4, zinc and double PHD fingers family 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the requiem/DPF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017545603.1">
        <property type="nucleotide sequence ID" value="XM_017690114.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EG47"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025766.2">
        <property type="protein sequence ID" value="ENSPNAP00000034191.2"/>
        <property type="gene ID" value="ENSPNAG00000023318.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108418170"/>
    <dbReference type="CTD" id="326933"/>
    <dbReference type="GeneTree" id="ENSGT00940000155070"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071565">
        <property type="term" value="C:nBAF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15691">
        <property type="entry name" value="PHD1_DPF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15530">
        <property type="entry name" value="PHD2_d4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000005">
        <property type="entry name" value="zinc finger protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025750">
        <property type="entry name" value="DPF1-3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888:SF15">
        <property type="entry name" value="D4, ZINC AND DOUBLE PHD FINGERS FAMILY 2,-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888">
        <property type="entry name" value="HL01030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14051">
        <property type="entry name" value="DPF1-3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="221"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="281"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="338"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="123"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="246"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034191.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="45087" checksum="AB130C9D30F4208F" modified="2024-11-27" version="2">MAAAVENVVKVLGEQYYKDAMEQCHSYNARLCAERSILMPFLDSQTGVAQSNCYIWMEKRHRSAGIAPGQLYTYPARRWRKKRRAHPPEDPALVFPPLKAAELELGLKKDVLGSLDGSSLEALLKGEPMDKRAPTEIRGPEEDSSQAEITGTTGASSHSTSTRVRKRILEPEDYLDDLDDEDFEEETPKRRKGKSKGRGVGNGKKKLEAAAAALEDRDKPYACDICGKRYKNRPGLSYHYTHSHLADEEGEDKEEAEIHPPAPQEETKTPKKGPNGLAPPNDYCDFCLGDSNMNQKTGQSEELVSCSDCGRSGHPSCLQFTAVMMAAVKTYRWQCIECKCCNVCGTSENDDQLLFCDDCDRGYHMYCLTPPMSDPPEGSWSCRLCLDLLKDKASIYQSQNTTME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EI06</accession>
    <name>A0A3B4EI06_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CS012 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C19orf12</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C19orf12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EI06"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035378"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028259.2">
        <property type="protein sequence ID" value="ENSPNAP00000035378.1"/>
        <property type="gene ID" value="ENSPNAG00000006427.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009077"/>
    <dbReference type="OMA" id="INTRELM"/>
    <dbReference type="OrthoDB" id="5976774at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033369">
        <property type="entry name" value="C19orf12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31493">
        <property type="entry name" value="NAZO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31493:SF1">
        <property type="entry name" value="PROTEIN C19ORF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20721">
        <property type="entry name" value="C19orf12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="14649" checksum="409D0BD2FF49B4BC" modified="2018-12-05" version="1">MAQRIQDIMRLCCEVSANQQMKAAVKGSGKGAAVAGGGAFLGGLLAGPPGIAAGGALGGLLGYWLTSGQFKPVPEIIMALPPQQQQKLYSDVMAILGTLDWVDVVQLVTLVMGNATMQQQVAAAVLSYVTKELKAEVRYGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EID6</accession>
    <name>A0A3B4EID6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Noggin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the noggin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EID6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036257"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030518.2">
        <property type="protein sequence ID" value="ENSPNAP00000036257.2"/>
        <property type="gene ID" value="ENSPNAG00000026946.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006009"/>
    <dbReference type="OMA" id="CMAERSC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.520:FF:000002">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.520">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008717">
        <property type="entry name" value="Noggin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10494">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN INHIBITOR, NOGGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10494:SF4">
        <property type="entry name" value="NOGGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05806">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008129">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="3 7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="153"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="159"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR008129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR008129-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036257.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="208" mass="23827" checksum="CC5122E742B438B1" modified="2024-11-27" version="2">FLLLEEGGILNSARAHLGTHYLRLRPSPSDHLPVPDLKEDRDPEYDPREQDLNEGTLRKKLGSHFDPGFMSVNAPLQLNVSARAPPSPPGAAPADFRRLDLTATPYGARLTLGKKARRKLVQFLWTYTHCPVLYGWKDLGVRFWPRYMKEGTCFSERSCSLPEGMFCKPVKSVTKTFLRWYCQGFLRQKYCTWIPVQYPIISECKCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EIY8</accession>
    <name>A0A3B4EIY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KANL3/Tex30 alpha/beta hydrolase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TEX30</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017558472.1">
        <property type="nucleotide sequence ID" value="XM_017702983.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EIY8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035181"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027845.2">
        <property type="protein sequence ID" value="ENSPNAP00000035181.1"/>
        <property type="gene ID" value="ENSPNAG00000024991.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430478"/>
    <dbReference type="CTD" id="93081"/>
    <dbReference type="GeneTree" id="ENSGT00940000163874"/>
    <dbReference type="OMA" id="EVFWLQG"/>
    <dbReference type="OrthoDB" id="6415022at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046879">
        <property type="entry name" value="KANL3/Tex30_Abhydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026555">
        <property type="entry name" value="NSL3/Tex30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136">
        <property type="entry name" value="TESTIS DEVELOPMENT PROTEIN PRTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136:SF11">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20408">
        <property type="entry name" value="Abhydrolase_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KANL3/Tex30 alpha/beta hydrolase-like" evidence="1">
        <location>
            <begin position="63"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="255" mass="27661" checksum="66D66205DA2F483D" modified="2018-12-05" version="1">MTQHTWKLRFRCRTHPLPLDGGATCSGASLKMVAVSEEKVTIPFGTKVLDGVLTVPEVEAVRTGLILTHGAGGDMNLKALRSLASCTAASGVLCLRFTCKSLNFTYRVRAFQAAVDYMKTLERFTLSNVFLAGRSMGARAAVALGRQMCAAEEGAIQGLVCVSFPLHPPGQRHRHVKRSEDLTALSHVPVLFVSGTADNMCERKLLEGVVEQMESSSSVHWVDGGSHGLAVKGRMEESVLEEVNSKIITWILEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EPR3</accession>
    <name>A0A3B4EPR3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SETD5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017553373.1">
        <property type="nucleotide sequence ID" value="XM_017697884.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000037940"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034871.2">
        <property type="protein sequence ID" value="ENSPNAP00000037940.1"/>
        <property type="gene ID" value="ENSPNAG00000030038.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427614"/>
    <dbReference type="CTD" id="55209"/>
    <dbReference type="GeneTree" id="ENSGT00940000157446"/>
    <dbReference type="OMA" id="XVSLLEY"/>
    <dbReference type="OrthoDB" id="1928087at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000009">
        <property type="entry name" value="SET domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462:SF1">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SET" evidence="10">
        <location>
            <begin position="312"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="193"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="456"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="575"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="836"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="952"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1067"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1098"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1175"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1283"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1452"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1505"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="505"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="581"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="652"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="676"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="688"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="845"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="894"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1188"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1210"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1238"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1283"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1301"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1334"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1363"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1382"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1515"/>
            <end position="1533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1533" mass="165920" checksum="2C214CC2BEA741C9" modified="2018-12-05" version="1">MSIAIALGVTTPETPYTDMAAGSDPESVEASPAVNEKTYSNHSCGSTQNHGYRGLPYADHNYGAPPPPTPPASPLSQTIIPRLELNGARSRFHTNDPNNSADSESSSEEEEGAVPSGWCQCRLMPDGYLIKCESCRGLDRRKGLDGQRRKADNVSVGESSATESGDEEVSAATVSYTATQHTPTSITLTVNRVKRSKAKKRKKSTEKTRATPKAKKVKAFREGSRKSLRMKNSTSEASGLDENTAEGWETRIRQWTDHYEEALANQYSADVQTLLQLHHSSSKNSASQPALTMDTINRTELACNNTVLSSQMQLQLGRVTRVQKHRKILRAARDLEPDTLIIEYRGKVMLKQQFEVNGHFFKKPYPFVLFYSKFNEVDMCVDARTFGNDARFIRRSCTPNAEVRHMIAEGMIHLCIYAVTQISKDSEVTISFDYEFNSCNYKVDCACHKGNQNCPVQKHNQSPSESMLPPSQHSLPTLPGAETRRRRARRRELEGSGHIGAVSDDSNHLLDEHGETQGASDAEEGLIDGMKQEEGHEDVDETGALISSRRSREDRKAEAIMHVFENLEKRRKRISQGLERGGPDDPKHEGVEPEEGRASSPIVANPGLNIGVMGVSTRRTSFTPDPAAASVETEKAPSAPLATPKPPPARTSKPRPKSRISRYRSGSAQRARRQRQALLAQQAAEAGAAGGEEGGIAAGPGDLGLGDGTLGPGQLQDGDCYGAASTALGGKASVRYPKTKKYLVTEWLNDKAPERVEEPVERPLRITTDPTVLATTLNMLPGLSHSPLICTTPKHYIRFGSPFNPERRRRPLTVDPAYGSCKKRWIKQAEDESMSFVSMEDGTESNSSHQSNSSSMASLTSRPELTAPFKKRKSKYAPETVAPPPDLLLRPLSPITPPLPSEPPVSSLHPLFSSCSLYLGAEEERQNGATLPYSPLPSLPASRCNTPLQFENISSPETSPVHRPESLSPEPCLRPDFDTPRIATFPDLSMTSSLGSPAPVSDDFPLAVQDPLGVSGGVSSLTPAPVSNAEASLGTRSNEAQAREQVFRTEFNLIYTCSPLNANLTTGPVTDRRHSQSEGSFSPAESYFSTVSGQALQAETGSGSLSPFPESHFGGGYPDSGTPPHTCNPPQKKKRAMGSGVSQLSGLPGYQALTVRGEFKPGQNMVSLLEYRKRKQGTRDHDPGGTMGTPTRPSSLCTSAESPGGPRSMLQPTASPQSSFSSPAHQAFPQIEEVSPPDLSSNIGNHSQTSGPRAPDSSQWMVPTSVERLREGQGVLERVLRGSLKMERVLKRPEGGVSEHVNSRDKDTDGMEGDRYDLPSSSLASPMRSPQRYSPSVHPHQVPPHLSEPHQHLESPAYHQQASSSPFRPTFSTSSSPSSSSVVQSYHQSRLGTPTPLPQDALSSAPPPSVDSRQTGGTQHHHSSSGGGMMDMVQLYSGNHVKASLLNSTLSGSVSMSSRGQGHPKTDSSIALCSQPLHGSRLTQPATVRSPGQAVLQASSRLLSASGAQHYPQRHLQQPPMQGSGVRTQSGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IHL7</accession>
    <name>A0AAR2IHL7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_037403432.1">
        <property type="nucleotide sequence ID" value="XM_037547535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IHL7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081942.1">
        <property type="protein sequence ID" value="ENSPNAP00000038800.1"/>
        <property type="gene ID" value="ENSPNAG00000015649.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411113"/>
    <dbReference type="CTD" id="561776"/>
    <dbReference type="GeneTree" id="ENSGT00940000153716"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="122"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="373" mass="43087" checksum="03F37A4B61CDDFB4" modified="2024-11-27" version="1">MAPSHVLRCCQRGLSWIPVIFINLVVCWSYYAYVVELCIYTIPNAEEQVIYLVVFHVCFVMFMWSYWKTIVSKPSNPSKEFCLPKAEKEQYEKEERPEAQQEILKKVARNLPVYTRTGSGAIRYCDLCQLIKPDRCHHCSTCDHCVLKMDHHCPWVNNCVGFSNYKFFVLFLAYSMLYCVFIAATVLQYFIKFWTLCRRRSVEHCPENQLPDNHAKFHVLFLFFVAAMFFISIMSLFSYHIWLVAKNRTTIEAFRAPVFRNGPDKNGFTLGFRKNLAQVFGDQKKHWFLPVYSSLGDGYTFPTRLVNTDSEQGIVDHQSSSKCSVDGQTNSRPLSDSQNHLLSNDVHSDEQKDCSVAKADNGLTVIVTVESES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2INS2</accession>
    <name>A0AAR2INS2_PYGNA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C9orf85</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2INS2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074300.1">
        <property type="protein sequence ID" value="ENSPNAP00000039687.1"/>
        <property type="gene ID" value="ENSPNAG00000033112.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033805"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="entry name" value="DUF2039"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876:SF5">
        <property type="entry name" value="CHROMOSOME 9 OPEN READING FRAME 85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876">
        <property type="entry name" value="ZGC:101016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10217">
        <property type="entry name" value="DUF2039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="70" mass="8144" checksum="1E52D190860A459B" modified="2024-11-27" version="1">MSSQKGGGSRSRTQKHQNTTAFKNDKYGASPQVKKANAKVHEGLCQRCKDILEWKVKYNKYKFLTQPRKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ISI8</accession>
    <name>A0AAR2ISI8_PYGNA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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            <taxon>Characoidei</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
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                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSPNAT00000049612.1">
        <property type="protein sequence ID" value="ENSPNAP00000040581.1"/>
        <property type="gene ID" value="ENSPNAG00000031328.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="122" mass="13346" checksum="6A5BA3A9ABC45D9E" modified="2024-11-27" version="1">MSGHLHLSVFKRDLLCIPKLSDPSRGRNSLPQNPLFSSSSSERWIKSTRLFYGLSGSTLDQTDTAESMRLEKQQQQCLQGLSADCRTGVECGGAELLWKLTDWNRVGGLTNQSTLGSSRGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITB0</accession>
    <name>A0AAR2ITB0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="primary">PPFIBP2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the liprin family. Liprin-beta subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2ITB0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056364.1">
        <property type="protein sequence ID" value="ENSPNAP00000042953.1"/>
        <property type="gene ID" value="ENSPNAG00000019659.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09563">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09566">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09569">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000007">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000017">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058914">
        <property type="entry name" value="LIPB1/2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037617">
        <property type="entry name" value="LIPB1/2_SAM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037618">
        <property type="entry name" value="LIPB1/2_SAM_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037619">
        <property type="entry name" value="LIPB1/2_SAM_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF18">
        <property type="entry name" value="LIPRIN-BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26022">
        <property type="entry name" value="CC_Liprin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="647"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="724"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="809"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="477"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="270"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1051" mass="118366" checksum="EA5D678AD4FDFC06" modified="2024-11-27" version="1">MASDASHMLEAALEQMDDIIAGSKAVVEFSNGLYDLGSPVSAGPLQVLQLAEELRVALELQAGDEGQETLRSQLPCSTAQTLLDWLEKGMVNLQSPPNNETYQQRLERLEGDKESLVLQVSVLTEQVEAQGEKIRDLEASLEEHHHKLISTEEMLQQELLSRTSLETQKLDLIDEVSYLKLKLVSMEEEHGHTDGDDKQHKAECVVNVISELQEQMRKFQQEITTRIQEQRALEAQGEGAPQQERDRSPDVGLGSSDFEDCGCRCRAAGENALQQELRVLKTKVDELESEKSQYERKLKATKAEISELQQLLASKDAEIESLQNQLLSRGNISNDSIEREEAYRRKLKDKYVELQRMKIGMESLLAANDEKDRHIEELTALLAQYRKNKDIMALSQVCGDRLLCGSSEEDLSGVLRLRPLAHKAHSDIVRSDLQMSTPRGPSPFLLPTVCLQKELESNCRGMQTSMENSVLCPGDTAFHNGQRSTQRMLSTSLEELQSGSKQRNRHVSDSSKYRTLPGKFSRPEQNGERDQYPSPLQLSPDESEDSEVNLRKTEKADESTLSDLSPVSSGIDSGHQSPVSPEHKKNQKGIRKLWGKIRRTQSGSLPADSMDSADFRRGGFRATTGPRLACVGSTDIACNVNTPFSKWSCDQVCTWLDDYGLGQYAPMARQWVSSGQTLLSASPHDFEKELGMKHPLHRKKLQLALHSFTTRLEEKSAELDHVWVTRWLDDIGLPQYKDQFHEGRVDGRMLQYLTVNDLLLLKVSSQLHHLSIKCAIHVLHINKFSPNCLKRRPGDEAQMQTSPSEVMQWSNHRVMEWLRSVDLAEYAPNLRGSGVHGGLVILEPRFNSDTLAMLLNIPPQKTLLRRHLATNFGSLVGSQAQLDKRENMESPGYTPLSTTAKVRPRKLGFSNFGHLRKKRPDDSTDYICPLEAAQAQALLNGTQRPYTGFRGLSPILDRDPERREQMLITKGAVMQIEALSEGINHLTESREEKSEPAGVSLSPNAKERLILQRHTWSLGERSHWEEEDMPAGRFHLSTMCTDERMTCLTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IW23</accession>
    <name>A0AAR2IW23_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IW23"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083783.1">
        <property type="protein sequence ID" value="ENSPNAP00000043933.1"/>
        <property type="gene ID" value="ENSPNAG00000001100.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156027"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002980">
        <property type="entry name" value="Na/ntran_symport_GABA_GAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF138">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GABA TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01195">
        <property type="entry name" value="GAT1TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="535"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="599"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="621" mass="69554" checksum="2578FB8B54C3E9C9" modified="2024-11-27" version="1">MASNGAKAAADGHVLTEVNEAPTSNDKPKTLVVKVQKKKDLPDRETWAGKFDFLLSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFLTLIFAGVPLFLLETSLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYYIVIIAWAIYYLYNSFTTELPWKSCDNSWNTDRCFSNYSLASTANMTSAVVEFWERNVHQLTKGLEEPGEVRVPLAITLAIAWVLVYFCIWKGVGWTGKVVYFSATYPYFMLLILFFRGVTLPGAKEGILFYITPDFEKLKESEVWLDAATQIFFSYGLGLGSLIALGSYNPFNNNVYKDSIIVCCINSFTSMFAGFVIFSIVGFMAHITQRPIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLMLGIDSQFCTVEGFITALVDEFPLVLRGRREIFIAVVCLVSYVIGLSNITQGGLYVFKLFDYYSASGMCLLFLVFFECISISWFYGVNKFYGNIEEMIGYKPCIWWKLCWVVFTPLIVAGVFLFSAIQMVPLTMNNYVFPKWGQGVGWCMALSSMVLIPGYMGYMFLTLKGSYKEVRINVLSPFRNIEKNCESRLIIKSGGVTPEKPRTSVIIIIMFIYLKCMYILEFKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYU4</accession>
    <name>A0AAR2IYU4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Cytochrome c oxidase subunit 5A, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 16">Cytochrome c oxidase polypeptide Va</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">COX5A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Mitochondrion inner membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1 16">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cytochrome c oxidase subunit 5A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYU4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056079.1">
        <property type="protein sequence ID" value="ENSPNAP00000042766.1"/>
        <property type="gene ID" value="ENSPNAG00000029859.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001424"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00923">
        <property type="entry name" value="Cyt_c_Oxidase_Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.40:FF:000002">
        <property type="entry name" value="cytochrome c oxidase subunit 5A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.40">
        <property type="entry name" value="Cytochrome c oxidase, subunit Va/VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003204">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036545">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200:SF11">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02284">
        <property type="entry name" value="COX5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48479">
        <property type="entry name" value="Cytochrome c oxidase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 16" id="KW-0349">Heme</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 16" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="16687" checksum="09135C581AF7D8CB" modified="2024-11-27" version="1">MRPPEIMFRAALRLSFSGARSLTRSRPQYTAPLAARCYSHGKQETDEEFDARWITYFSKPDIDAWELRKGMNTLIGYDLVPEPKILDAALRACRRLNDLASAIRILEAVKDKAGPHKEIYPYVVQELRPTLSELGIPTPEELGIDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J4T7</accession>
    <name>A0AAR2J4T7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 8">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J4T7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082431.1">
        <property type="protein sequence ID" value="ENSPNAP00000044841.1"/>
        <property type="gene ID" value="ENSPNAG00000009698.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163144"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000060">
        <property type="entry name" value="Parvalbumin, thymic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF12">
        <property type="entry name" value="PARVALBUMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="110" mass="11661" checksum="542C8B854B5D6E2F" modified="2024-11-27" version="1">MAFAGILKDEDIAAAIADCAAADSFNYKTFFAKAGLSAKSSDDIKKAFFVIDQDKSGFIEEDELKLFLQNFSAGARALTDAETKAFLAAGDSDGDGKIGVDGIQFNGKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J6Q1</accession>
    <name>A0AAR2J6Q1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">POPDC1-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">POPDC3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J6Q1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071269.1">
        <property type="protein sequence ID" value="ENSPNAP00000045909.1"/>
        <property type="gene ID" value="ENSPNAG00000016050.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002563"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051146">
        <property type="term" value="P:striated muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006916">
        <property type="entry name" value="POPDC1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055272">
        <property type="entry name" value="POPDC1-3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101">
        <property type="entry name" value="POPEYE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101:SF18">
        <property type="entry name" value="POPEYE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04831">
        <property type="entry name" value="POPDC1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="POPDC1-3" evidence="7">
        <location>
            <begin position="29"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="336" mass="38064" checksum="3BE63581B316C649" modified="2024-11-27" version="1">MTMDPPSLSGRANYSVSDQPLCSEWQKAPEGAVFHLAHILLVLGFMGGSGFYGLLYMFSFLALGFLCCCLHGWSEPCAPDASAWPCALSVLCVAQALHVAHRARSVAFGGELQELYARMFNKLGVSLTHYGEIVACCEGQIQTMEKDHFFSIEGKTPIDKLSVLLSGRVRVSVNGEFLHYIHPFQFLDSPEWDSLRPSEDGVFQVCCHQRFHSYILCDLSCWMPKISFLFFSLLQVTLRADSRCRYVTWRRKKLYLLFAQHRYIARIFALLVRNDIADKLFSLNTAALDGRGFRYDLRLPSFCHGPHLERTGAIPPLRPSRNRNKRVITKPVDSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J8F3</accession>
    <name>A0AAR2J8F3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J8F3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064164.1">
        <property type="protein sequence ID" value="ENSPNAP00000046597.1"/>
        <property type="gene ID" value="ENSPNAG00000034171.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286925"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="129"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="585" mass="66990" checksum="A267D33AFFEFC6BB" modified="2024-11-27" version="1">MHVSLSLLIVKQRYFEFGDKPQRLLVRQLRGFQANRAIHQIKSPSGVLITDPIAINDCFREYYEKLYQTKAKGDVKTWLKNFKLPKLDEAAQRALDSELTSEEIIDSIKSFPSTKNYKITPLLLRMFIHSTKLGTLPKTLYAAHISLLLKPGRDKTDLSSYRPIALLNTDLKILSTVLANRLKQYITSIIHPDQTGFIPNRFSFFNVRRLMDIIYHKYHGVSAQGVLSLDAEKAFDQVEWGYIIATLKEFGFGHAFISWVQMLFEQSPYFSLYRGTCQGCPLSPLLFSIAIEPLAISIREDENIKPIILGGVEHKLPLYADDIILLSEPEQSIPHLLKLIGLFSEKSGYTINWQKSEFMALSANHNPRFLRSLPFKISTVKIKYLGIEVTKNPNVLFKHNFAEKMNRLKQDIDRGRILPLSLIGWVNAIKMVSLPRFFYPFQNIPIFIPKSFFESLDSIMPFIWGFKTHRISKIHKPRSLGGLGLPCFLHYYWVANARALAYWQYGYKEGISLDLPPWLAVEKDLIVDSSLLALFSSPRAPSALKKLGKLAIFQNLSYLPQFGTIIHSHPLYLIVHFVTGLKKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JCB1</accession>
    <name>A0AAR2JCB1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Leucine-rich repeat-containing protein 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Photoreceptor-associated LRR superfamily protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Retina-specific protein PAL</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Photoreceptor synaptic protein essential for normal vision. Involved in synapse formation in cone photoreceptor cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Endomembrane system</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017570792.2">
        <property type="nucleotide sequence ID" value="XM_017715303.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JCB1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054489.1">
        <property type="protein sequence ID" value="ENSPNAP00000047967.1"/>
        <property type="gene ID" value="ENSPNAG00000036584.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108437888"/>
    <dbReference type="CTD" id="553432"/>
    <dbReference type="GeneTree" id="ENSGT00940000156033"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000058">
        <property type="entry name" value="immunoglobulin superfamily containing leucine-rich repeat protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000744">
        <property type="entry name" value="Leucine rich repeat, Ig-like and transmembrane domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000928">
        <property type="entry name" value="Leucine rich repeat, Ig-like and transmembrane domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF9">
        <property type="entry name" value="LEUCINE-RICH REPEAT, IMMUNOGLOBULIN-LIKE DOMAIN AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6 23" id="KW-0732">Signal</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0844">Vision</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043322150" description="Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 1" evidence="23">
        <location>
            <begin position="21"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="554"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="250"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="457"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="653" mass="71575" checksum="673EAB0BBFB482C6" modified="2024-11-27" version="1">MLGRVGFCLVFCCLPLLCSACPAQCSCFYHKLSDGSKARSVLCNDPELTLVPTNFPTDTSKLRIEKTAISRISPETFHYLSSLEFLWMSFNSLSKLHSDSFRGLYALDELRLDGNFITSFPWDCLTDMPNLRLLDLHNNKITSIPAEATIYIRNLTYLDLSSNSLTTVPAEVLTMWFSPKLTQDTENSKMILGLHDNPWQCDCRLFDLVQFQKSPSSSVAFIDTRLRCSEPESLSGVLFSDTELRKCQSPRVHTAVARVRSSVGNNVLLRCGTIGVPIPELSWSRADGKPLNGTVQQEVSKEGIIWSVLSVPAVSYHDSGKYVCKATNFVGSADAIISLVISDTWRIDEAVVKKTRGKKTGGFGRAAYQEKLIARYVPPTTSSAAVPIIEPLNRPKSTASFEIESYSISDGISAVKMTVPPETGTGTDGHSIEVEKEVLSNLAGNASSLQQGPEKRVVRSVKVIGDTDHTVSLNWRAPAATNTTSFSVLYAVFGERDMRRINVGPGKNRVTIDGLVPKTKYIACVCVKGLIPKKEQCVIFSTDEAASASGTQKLINVVVITVACVIAVPLTLIVCCGALKRRLQKLLGRKSKDIQDSYVTFETLSPGTKAKGLEGEYLTRLNQDESNRLLSARSSVDSEATARTEGQPNEYFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JF26</accession>
    <name>A0AAR2JF26_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Bromodomain containing 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the BET family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000042306.1">
        <property type="protein sequence ID" value="ENSPNAP00000048932.1"/>
        <property type="gene ID" value="ENSPNAG00000015972.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162790"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05497">
        <property type="entry name" value="Bromo_Brdt_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05498">
        <property type="entry name" value="Bromo_Brdt_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000003">
        <property type="entry name" value="Bromodomain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.220:FF:000001">
        <property type="entry name" value="bromodomain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000002">
        <property type="entry name" value="Bromodomain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031354">
        <property type="entry name" value="BRD4_CDT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043508">
        <property type="entry name" value="Bromo_Brdt_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043509">
        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF245">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17105">
        <property type="entry name" value="BRD4_CDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="137"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="451"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="8">
        <location>
            <begin position="752"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="573"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="639"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="826"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="950"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1167"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
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            <begin position="374"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JH56</accession>
    <name>A0AAR2JH56_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JH56"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076229.1">
        <property type="protein sequence ID" value="ENSPNAP00000051285.1"/>
        <property type="gene ID" value="ENSPNAG00000034278.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182912"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF25">
        <property type="entry name" value="ZINC FINGER (C2H2)-60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="346" mass="39465" checksum="3C0AB22BF43BF580" modified="2024-11-27" version="1">MPETVEQLHKTVKDTFQIHEEFILHYFDEDFGDFFTIHSTSDVKHKGSIKVVIIPSVVLTLAIPETDSVTDADNVRDTPSVTSQMSSEPSTSEDTRPDGTSSASSQDTILLSPQKELWPKEIKIPLFTVATEAVLRSANEQFLKDGTVLNNTRVRSEIMEKLADYMYSYTAYPTGLQVGEVAEALVKKHPCLTEPGSRNGWIGWMYSLKYKMGNYRSKLRNLGFPEVACNSLNNKRPGERKPAQNVKKARKGEVKYLPHYPSGENGEHQEQQRLKILSEIKKRERTTINQLMSKTFAHRRQDVVTLQLSVKEIKDRWPALLDVSQVNAEFHRITNVNLESKFMFMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JHG4</accession>
    <name>A0AAR2JHG4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MAFF</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560970.1">
        <property type="nucleotide sequence ID" value="XM_017705481.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JHG4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074947.1">
        <property type="protein sequence ID" value="ENSPNAP00000049762.1"/>
        <property type="gene ID" value="ENSPNAG00000037147.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108431979"/>
    <dbReference type="CTD" id="23764"/>
    <dbReference type="GeneTree" id="ENSGT00940000161112"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045604">
        <property type="term" value="P:regulation of epidermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14717">
        <property type="entry name" value="bZIP_Maf_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000011">
        <property type="entry name" value="Transcription factor MafG, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF25">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAFF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="51"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="42"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="159" mass="17627" checksum="21F8D4E5BFF33CC9" modified="2024-11-27" version="1">MTSDGRASKALKVKRECGENVPVLTDSELMSLSVRELNLLLRGLSREEIQKLKQRRRTLKNRGYAASCRVKRVSQREALEQQKKELQREVERLGAENAGMRRELEGLGARLAALQRFARGLEAGGGTLLATAPRLNTASVITIVKSPQQSHAHRGQEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLH6</accession>
    <name>A0AAR2JLH6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLH6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067437.1">
        <property type="protein sequence ID" value="ENSPNAP00000050706.1"/>
        <property type="gene ID" value="ENSPNAG00000032042.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286933"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF134">
        <property type="entry name" value="TRANSPOSABLE ELEMENT TC1 TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13384">
        <property type="entry name" value="HTH_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="145"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38210" checksum="3461ED38942C52D4" modified="2024-11-27" version="1">MGKQRDLSTGIVAQIQALRQQGLTQTKIAEQLGISQSSVCKALKKNCSKRTNCSGVRKTSARDNKQLRKIAVSNRFKSTSELTDLWNKQTGADVSRSTTYRRLRELGFKSRVPAVKPMLNKKQMEKRLKWAKEHSEWTAEDWQKVVFSDESRFCISIGDQGPRVWRRGGETYNHECVKRSVKFPQSVMVWGCMSARGEGKLCFLKKTVNAAVYQDILETFLIPTVEEQFGEEDFIFQQDHAPAHAAKSTKDWFTKKQLEVLAWPANSPDLNVIENLWAIVKRKIRDRKPTTLDQLKQNIATAWEVVSAETCDKLVKSMPRRLQAVIQAKGAATKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JRG2</accession>
    <name>A0AAR2JRG2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SPTB</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000073628.1">
        <property type="protein sequence ID" value="ENSPNAP00000054608.1"/>
        <property type="gene ID" value="ENSPNAG00000032102.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154656"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000057">
        <property type="entry name" value="Calmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
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        <property type="match status" value="3"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
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            <begin position="33"/>
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            <begin position="188"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JS99</accession>
    <name>A0AAR2JS99_PYGNA</name>
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        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
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        </reaction>
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        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
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        </cofactor>
        <text evidence="9">Probably binds two magnesium or manganese ions per subunit.</text>
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        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:base-excision repair"/>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
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        <property type="entry name" value="AP_endonuc_1"/>
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    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
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            <position position="61"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03372"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0AAR2K012</accession>
    <name>A0AAR2K012_PYGNA</name>
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        <recommendedName>
            <fullName evidence="20">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="20">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="26" type="primary">BTK</name>
    </gene>
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        <name type="scientific">Pygocentrus nattereri</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2K012"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059957.1">
        <property type="protein sequence ID" value="ENSPNAP00000055456.1"/>
        <property type="gene ID" value="ENSPNAG00000018069.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158469"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01238">
        <property type="entry name" value="PH_Btk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000052">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000040">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001562">
        <property type="entry name" value="Znf_Btk_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00779">
        <property type="entry name" value="BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00107">
        <property type="entry name" value="BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51113">
        <property type="entry name" value="ZF_BTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="7 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 17" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 20" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="24">
        <location>
            <begin position="20"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="23">
        <location>
            <begin position="199"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="22">
        <location>
            <begin position="266"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="388"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="167"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="648" mass="75093" checksum="B88AD182E812550F" modified="2024-11-27" version="1">TEKKLQIKGYGGAVWMEKHKNILEDIFIKRSQQKKKTSPLNFKERLFVLTQDKIAYYDYDFEKGVRLKGSVDIDKIKCVEIVIPEDNAPPERLFPLQIVYDEGPLYMFAKTDDVRKQWIHKLKHVVRFNKDLMQKYHPCFWEDGMWKCCQQEVKQAVGCRVLETRNGGSRRKSRKPLPPTPEEPIRPLPPQPPKQPGFSVGMTVIAEYDYSPMAPQDLELRKDEEYTILQICDANWFRAKDQYGNEGYIPSNYVVEALNGLEKFEWYCKNMNRSQAENLLKTENKDGGFLVRDSGRYTGKYTVSVFTKGGGENVGSCRHYNILTTQQGLFYLAEKHYFSNIPELINYHQHNAAGLVSRLKYVVSNRTQNAPSTAGLGYGVWEIDPRQLTFIRELGNGQFGVVKYGKWQGRHDVAIKMIKEGSMSEDDFIEEAKVMMKLRHENLVQLYGVCTKQRPIYIVTEFLPNGCLLTYLREDLQHPSAVQLLEMCKDVSEGMAYLESQQYIHRDLAARNCLVDSNGTVKVTDFGLSRYVLDDEYMSSAGSKFPVRWSPPEVLLYCKFSSKSDIWAFGVLMWEIYTLGRMPYERMNNTEIVDKVAAGYRLYRPQMANDRIYSVMTTCWHEKADERPTFQDLVIIIQDVLDNLYETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K2U2</accession>
    <name>A0AAR2K2U2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RBM20</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037390176.1">
        <property type="nucleotide sequence ID" value="XM_037534279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K2U2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068670.1">
        <property type="protein sequence ID" value="ENSPNAP00000056812.1"/>
        <property type="gene ID" value="ENSPNAG00000018265.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413954"/>
    <dbReference type="GeneTree" id="ENSGT01030000234642"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12685">
        <property type="entry name" value="RRM_RBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034790">
        <property type="entry name" value="RBM20_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-met"/>
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        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
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            <begin position="467"/>
            <end position="542"/>
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            <begin position="1050"/>
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    <sequence length="1102" mass="120819" checksum="E918AAA4FC83E92B" modified="2024-11-27" version="1">MKQAWDKSVFENGQTKHKHPGTGHVDSGTAAPLEHLQSSGSGDKLDKKAVSVGGALGGAGQNLLLSPASIQLAQLQAQLTLHRLKLAQTSSTAAAATVLNQVLSNVAMSQPLFNQLRGSCIGQAHGRGFPPAPITFPPPSTTLGPLAGGGFTQNHAGIRLNHYGAGSSNQNTNQQGEYGKKAGMFQTDTDRRVQFGFTGIGPSKVGDAGQYGGPGVQAKNSTQNNYQRDCYPPEGQQGVFVGGSGELIQDSGQKEQWKNPTSFPLSGKLDMGTGAGTSTWGPAAQGFVGPRAELYNPEEPTGEPRFSPAGGLAFNFTAGQPGFVGYQQQQKGEEGKGMTLQAHQLNDYHGATPSHLPHQCTICEKKVYNLKDWDQHVKGKLHLQNRLLYTEAPAVGALHFPAASEGCPGSALTNAMAYSSPASQDVSAGGASYLPATAMKSFPLSGAGFTSHQPGAKFPQSKACPGRVVHICNLPEGSCTENDVINLGLPFGKVTNYILMRSTHQAFLEMAYVEAAQAMVQYYQLQPAKINDQKLLIRMSKRYKELQLKKPGKDVESIIQDINSQRERGEMQDMDRYLPERARSRSPISRSLSPRSHSPSFTSCSSTHSPQGAPCRPDWINGLGPRRGSWDWTSHGRRDEDPRDRDEWRNGEDERPNGRLSERRKLYLKPGERGSPRMDERGRDWYPRGSPQGPPFSYHTMDDFYKKEPTYKAEKTLRTPQNQRHEGKYKRRDGTDHHRARHSESDLTEDMGSSRAAEERGRSKRTSRRQEREDKETESSKEATEHRAKDRSSSPRNSKTSEPTECERDKDSEVEEWVSGEETEGESWYPKSMEELVTVDEVGGEDDSIIEPDLPDLQEEEPAEEPSQPASSPNPNPSAEVPALAEKTQEEPLPPPDTSPEETSSLSLAESREPSVSSSAPDQPCSQLCLFPSQEFKSALEESSHTEPQTSRAAPDSPPSHGRASEMACEREKTADVQTAGEAVGPAQTDKKETQHIEENQRRDALISVPADSPQRTDTETPSPSREQEKIISEYSIPLGVEFIVPRTGFYCKLCGLFYTSEETAKTTHCRSTVHYRNLQKYLSQLAEESLLHFHADPASTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K9T4</accession>
    <name>A0AAR2K9T4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nibrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NBN</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Nibrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K9T4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073258.1">
        <property type="protein sequence ID" value="ENSPNAP00000061073.1"/>
        <property type="gene ID" value="ENSPNAG00000027101.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000521"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17741">
        <property type="entry name" value="BRCT_nibrin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FHA_NBN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000017">
        <property type="entry name" value="Nibrin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nibrin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10980">
        <property type="entry name" value="Nibrin, BRCT2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRCT_dom"/>
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    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040227">
        <property type="entry name" value="Nibrin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032429">
        <property type="entry name" value="Nibrin_BRCT2"/>
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    <dbReference type="InterPro" id="IPR043014">
        <property type="entry name" value="Nibrin_BRCT2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013908">
        <property type="entry name" value="Nibrin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016592">
        <property type="entry name" value="Nibrin_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12162:SF0">
        <property type="entry name" value="NIBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12162">
        <property type="entry name" value="NIBRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08599">
        <property type="entry name" value="Nbs1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16508">
        <property type="entry name" value="NIBRIN_BRCT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011869">
        <property type="entry name" value="Nibrin_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01348">
        <property type="entry name" value="Nbs1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0469">Meiosis</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="893"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="922"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="949"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="976"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="382"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="720"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="762"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="806"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="392"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="408"/>
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        </location>
    </feature>
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        <location>
            <begin position="422"/>
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        </location>
    </feature>
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        <location>
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    </feature>
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            <begin position="506"/>
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        </location>
    </feature>
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        <location>
            <begin position="529"/>
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        </location>
    </feature>
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        <location>
            <begin position="548"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="629"/>
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        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="762"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020013"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1018" mass="112208" checksum="BBC6D7C1C9530D5C" modified="2024-11-27" version="1">MWKLQPAESESGVFYLFPGKEYVVGRKNCDILLANDQSISRVHAKLTVTEQTVTLKDSSKYGTFINDEQLAAGSTKTLRGGDRVTFGVFQSKFRLELNSVVVCSSCVDNEGKLALSQAVQSVGGRLVSSWSQDCTHLVMPTVKVTVKTICALLCCRPIVKPEFFTELSQAVQRKETFPKSENFKPEMDEPSLAKQEVDLGPRPERKSLFKDKTFVFLNAKQMKRLSQAITCGGGRSQLLEEGSLPVSLLESAGTCVLDVGTGSSQALIPSTTKKWTDSVGQILHRNGLRFITESEIGLAAIYVSNQTYCNPCSSMDSESVRMRSAIPAATLTQNTTVDETVLPAASQNITAYVANTETSQSFSRMHGCGAIAVGETPEKCPVQAEPLRSAKPASTLSRRQLSTMEPPTTSESVKSSLTSSERVQEKKAESASSDRRNDDCVKFPDPLNSSATLKQSPQKQSALTSYFQPVSKKRSREHGADSELSEAKLSRRECEGEENEKKNATPAASLQTSHTSQKRHGTTPQSNFSASLGLGSALAMSSGPSGDHNAQNYDSNPISGSKKRKEPVQDQPSNLGVQEERTDLDDSLEELESIMSEDMDEPMQPAASKKQRLGQGESSTSSKERNPKQYHNTISSNQQRVEQQSIASKSQNLQQGVPDGANKHQNSKRKLQGSGRAADLEQDQLGSVHRKDRSESDNVKEEEVSFVEISEALNGFAKHSEVPAVKQESKGSSSASASENDTELPQRLLQVQFKCLTVNAPSRTRSDPLQNRDPNGKNFKRFRKVPVPGLHGLPSIIGGSDLVAHNRSKNTELEDWLRDAAEEEKRQEREETLGDDLFRQTRLSLAISLYRLFELWTLSQSSIERESIIWRDRQKCIQSDFPSLFLSLTLSPLAYLTLSLFSLHLFLSCFSFFFCLFPTCSLFLCLCPICSFSSCLLLSYAVFCSSCALSPFFSICLAFFLFCSCVCFLSLLRFLFLFSFSITVFLLPVTLSLFLSLSFPLSLSRSVPSCISFLPPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KBE1</accession>
    <name>A0AAR2KBE1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Protein kinase C</fullName>
            <ecNumber evidence="27">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="37 38">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="37 38" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="37" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="31">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="31">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="27"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KBE1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045362.1">
        <property type="protein sequence ID" value="ENSPNAP00000059767.1"/>
        <property type="gene ID" value="ENSPNAG00000023614.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155217"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20833">
        <property type="entry name" value="C1_cPKC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20836">
        <property type="entry name" value="C1_cPKC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04026">
        <property type="entry name" value="C2_PKC_alpha_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05616">
        <property type="entry name" value="STKc_cPKC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000012">
        <property type="entry name" value="Kinase C alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000023">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000080">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000006">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000031">
        <property type="entry name" value="Protein kinase C alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034664">
        <property type="entry name" value="cPKC-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014375">
        <property type="entry name" value="Protein_kinase_C_a/b/g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000550">
        <property type="entry name" value="PKC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="17 27 30" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 31" id="KW-0106">Calcium</keyword>
    <keyword evidence="18" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0391">Immunity</keyword>
    <keyword evidence="14 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 31" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 27 32" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="23" id="KW-0804">Transcription</keyword>
    <keyword evidence="20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 27" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="35">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="35">
        <location>
            <begin position="131"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="33">
        <location>
            <begin position="188"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="34">
        <location>
            <begin position="364"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="36">
        <location>
            <begin position="623"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="28">
        <location>
            <position position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <begin position="370"/>
            <end position="378"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30 32">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRNR" id="PIRNR000550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="695" mass="79364" checksum="3DB979684A25F057" modified="2024-11-27" version="1">MVVGVSLSVVCGAVPRRAQQKRQRCRREEAVRMADLAQSGSDGEQRARGFARQGALRQKNVHEVKDHKFIARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKVHTYSSPTFCDHCGSLLYGLIHQGMRCEHCMMNIHKRCVANVPSLCGTDHTERRGRIQITANIKNNVLTVIKEAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCCLNPTWNETFTFNLKDSDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKQGVDGWFKLLSQEEGEYFNVPVPPEGEEGNEELRQKFEVRVQLSISSNAISKFDSNGNRDRMKLSDFNFLMVLGKGSFGKVMLAERKGTDELFAIKILKKDVVIQDDDVECTMVEKRVLALSGKPPFLTQLHSCFQTMDRLYFVMEYINGGDLMYQIQQVGKFKEPHAVFYAAEIAIGLFFLHRKGIIYRDLKLDNVMLDAEGHIKIADFGMCKENMLEGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQPPFDGEDEDELFQSIMEHHVSYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIREHGFFRYMDWEKLENKEVQPPFKPKACGRDAENFDRFFTRHPPVLTPPDQELFIMNLDQDEFLGFSYTNPEYVPPNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KF62</accession>
    <name>A0AAR2KF62_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KF62"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087974.1">
        <property type="protein sequence ID" value="ENSPNAP00000061077.1"/>
        <property type="gene ID" value="ENSPNAG00000031753.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164961"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="282"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="415" mass="46885" checksum="A0AE8BA2626578A1" modified="2024-11-27" version="1">MPVRPGKPKRIVRVCWERLAEEPVRLIFNSHLRQNFDQISGEVGDIDSEWAMFRSSIVEAADCSCGRKVVGACRGGNPRTRWWTPQVRDAVKLKKESYRTWLACRTPEAAGRYRQAKRSAASVVAKAKTRVWEEFGEALESDFKSAPKRFWQTVRRLRRGKQCATSTVYSGDGVLLTSTEDVIGRWKEYFEDLLNPTDTFSSEEAESGDTGIGLSITEAEVAKVVKKLLGGRAAGVDEIRPEFLKALDVVGLSWLTRLFNIAWTSGVVPLDWQTGVVVPLFKKGDRRVCSNYRGITLLSLPGKVYARVLEKRVRLIVEPQIQEEQCGFRPGRGTLDQLFTLSRILEGSWEFAQPVHMCFVDLEKAFDCVPRGILWEVLREYGVHGSLLRAIQALYKQSRSLVCMAGSKSDFFPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KGI5</accession>
    <name>A0AAR2KGI5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the paired homeobox family. Unc-4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017562950.2">
        <property type="nucleotide sequence ID" value="XM_017707461.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KGI5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049714.1">
        <property type="protein sequence ID" value="ENSPNAP00000061574.1"/>
        <property type="gene ID" value="ENSPNAG00000031492.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433113"/>
    <dbReference type="CTD" id="340260"/>
    <dbReference type="GeneTree" id="ENSGT00940000161420"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000057">
        <property type="entry name" value="Short stature homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46799">
        <property type="entry name" value="HOMEOBOX PROTEIN UNC-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46799:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN UNC-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="99"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="101"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="251"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="365"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="458"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="469" mass="50716" checksum="20737F8D53E23711" modified="2024-11-27" version="1">MMDSRILDPPHAQFGGSLGGMVSFPYHLSHHPVYELAGHQLQSAAAAVPFSIDGLLNGSCTASVVNSNPLLSTGCGMNGDSQQYKLTDSGDPEKDSPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLIESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDAEEIARRERERLEKKKRKQERRLLRAQGKLVPADLFHTPGSDSDSGVSQITDGELNALPCESSRSHARSACEPAAPKLQDQREAGAAELDSADGGRQASLGHGTKPSPLQKLNPFSVESLLADSRPRRKPSVDFPVSPTRPLIGKGHFLLYPITQPLGFIVPQTALKSPAAAEAEKAAAADCADAAGHHHRSNRESGSASPKSQSAEACRTARSPQCSPARAVNGHSPGLCGEPSAQEKEAELSSPASPQQPERREPAALLDYHSAQTAASSTDQDKDSVEVDMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJZ2</accession>
    <name>A0AAR2KJZ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KJZ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046940.1">
        <property type="protein sequence ID" value="ENSPNAP00000064643.1"/>
        <property type="gene ID" value="ENSPNAG00000032594.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF3">
        <property type="entry name" value="CALCYPHOSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="91"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="184" mass="21015" checksum="74B1D59E244B87CD" modified="2024-11-27" version="1">MEELSLSKQVQTNTNKIIKNTSNKKAASNPPKINLNQYLLFLKKSNAYNALEHNVYAVYTVCQDYDAVWRTVGQQGGPLVDYAEMMRAVTGEMSEIRKVYVKLDPNKTGYISLIDIEKFYSAKVNLHLEHGETLSGKFHSQCSYTKVVTKDVSYAEFEDYYQGLSIEISNDQEYINILKSTWGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKJ3</accession>
    <name>A0AAR2KKJ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ferric-chelate reductase 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FRRS1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKJ3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064319.1">
        <property type="protein sequence ID" value="ENSPNAP00000064815.1"/>
        <property type="gene ID" value="ENSPNAG00000021614.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157704"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09628">
        <property type="entry name" value="DOMON_SDR_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08544">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4060:FF:000003">
        <property type="entry name" value="Ferric chelate reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4060">
        <property type="entry name" value="Reeler domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051237">
        <property type="entry name" value="Ferric-chelate_Red/DefProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002861">
        <property type="entry name" value="Reeler_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042307">
        <property type="entry name" value="Reeler_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45828">
        <property type="entry name" value="CYTOCHROME B561/FERRIC REDUCTASE TRANSMEMBRANE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45828:SF3">
        <property type="entry name" value="FERRIC-CHELATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02014">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51019">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043770250" description="Ferric-chelate reductase 1a" evidence="12">
        <location>
            <begin position="24"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="591"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Reelin" evidence="15">
        <location>
            <begin position="14"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="13">
        <location>
            <begin position="225"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="14">
        <location>
            <begin position="345"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="68919" checksum="D3121A00E934B8F6" modified="2024-11-27" version="1">MEVPVKVWALLVLLSVCLKTVLCFSNGKVEQACDDMTPEHGHQSSPHPPPYSITVDKSKFSPLEQIKVTLSAVPSENKAHFKGFLIEARNASNPNAGPVGCFTLLDPEISQLLRCNDREGAAVSHTSEVSKTEVQVIWTAPQDPPSSVQFQVTVVQKYKLYWVRIQGPVVSLNDAPPLPPLPTAGQSVAKTTTAASILSDAFTSDDCGIKKSCLREPAGCDPWKDALCYFLSFRTEDASVVFELSGPAPGYISFALSTDKWMGEDDVYLCIKEDDRVDISAAYVQGRTHPVIASENVLRDTAWRLADGVIQCRFRRNIRIPATYQNRSDLGEAHYLFMAHGRAEGGRTHRHDRQPLISTNPVLITGLPEDLTGSRSHLLIKFHAVFMLIAWMTTVSTGVIIARYFKPDWAETTLCGQRVWFQFHRALMLVTVLLTCIGFILPFVYRGGWSKRAGSHPYLGCTVMALALIQPIMALFRPAPDTSRRYVFNWLHLGTGMIAQVIAVVAICLGIHQQALLLPAPWSTGVLAGYVVWFVLADLVLEVHRRGLFSIGQFVKSFEIHTENIQTEDREEILFVPSENESYIKVRHCKYYATLLLLILSRKINLIKFFFLVLGALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KR55</accession>
    <name>A0AAR2KR55_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SMARCD2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMARCD family.</text>
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        <property type="nucleotide sequence ID" value="XM_017682063.2"/>
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        <property type="protein sequence ID" value="ENSPNAP00000064887.1"/>
        <property type="gene ID" value="ENSPNAG00000011770.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000158654"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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            <end position="352"/>
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        <location>
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            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="58"/>
            <end position="93"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="500" mass="56284" checksum="AE4BEB249BBDFD83" modified="2024-11-27" version="1">MASRAGFLPSSPAGPGGANPGAAGPPGGVRMAGVPPSAGGYRGLPSPAGQYPRPGMPPSRSMPMGGIGPMGSPMPGPSYGSGMPGRPGMPHSSLDPARKRLLQQQAGGLMGPRRGIKRRKMADKVLPQRIRDLVPESQAYMDLLAFERKLDQTIARKRMEIQEAIKKPITQKRKLRIYISNTCTPGKPEGEEAEKVASWELRVEGKLLDDPGKQKRKFSSFFKSLVIELDKELYGPDNHLVEWHRMPTTQETDGFQVKRPGDVSVKCTLLLMLDHQPPQYKLDPRLARLLGVHTQTRASIMQALWLYIKSNKLQDCHEKEYINCNRYFRQIFACPRMRFSEIPMKLAGLLQHPDPIVINHIISVDPNDQKKTACFDIDVEVDDPLKSQMTSFLSSTTNQQEIAALEMKIHETIESINQLKTQRDFMLSFSNNPQDFIQDWLKSQSRDLKLMTDTAGNPEEERRSEFYQAPWVQEAVGRYVFSKVQQRRQELEQVLGIRLT</sequence>
</entry>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
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            <taxon>Teleostei</taxon>
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            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KXJ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075149.1">
        <property type="protein sequence ID" value="ENSPNAP00000067204.1"/>
        <property type="gene ID" value="ENSPNAG00000034318.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014593"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 95"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN C9ORF135"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15139">
        <property type="entry name" value="CFAP95"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="200" mass="23296" checksum="5C00E5C263E52F4E" modified="2024-11-27" version="1">MYQPSQLGMNYSRTLVSNWHQNREAEPKDYDLTACRDGQKKLHKSTYKHFGTCLDADWSTTTETHMSQYLLKKEYEAKETPKSMVQASSFQSLVFDREAGHTGCKSILPRHRPDYNDMELCTVYALDYLPPRATTKEAVPVKAVMDKMVDFRRRQSHFTDVADHRRQGRNTWQDMNEAQAKVCGVMKPLYTDMDMYPSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        </lineage>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L301"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087540.1">
        <property type="protein sequence ID" value="ENSPNAP00000068756.1"/>
        <property type="gene ID" value="ENSPNAG00000036424.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234815"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="127" mass="14672" checksum="0DAE5E37738E95FB" modified="2024-11-27" version="1">VIHGTLFSVLQCCLQRPNTLSRRTYAHLLLCVGISPLRQRFILFFSFSFSYFLFHCSAVSSRLIEAVFLIVLALRGIDLVSASLKPEGEQQMNMVVLQFPPRESCRIRVILLSRYGTWVFCLHNKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L6Q1</accession>
    <name>A0AAR2L6Q1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L6Q1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055911.1">
        <property type="protein sequence ID" value="ENSPNAP00000070379.1"/>
        <property type="gene ID" value="ENSPNAG00000036605.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein phosphatase 1 binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PBD_PPP1R3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBM21_dom"/>
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        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF2">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBM21"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="121"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="277" mass="30812" checksum="273CA6090772D99B" modified="2024-11-27" version="1">MEPAVGEVWSSSQDGSNLLGLPGPCPWEDDEEPLGLGGIKPKSSPIPRRRSSASSCSDDDSQPPPSSSRRVSFADAFGLSLVSIKQFDSWAPDPLEADLNEVKEYFLSQLFTLPATPEELAQKVQENKLELESLEILPGTTTLKGIIRVLNLCFDKLVFIRTSLDCWSSHFDLLAEYVPGSSNSNTDCFSFRLTLVPPFGKQGARVDFCLRYETPFGTFWANNSGQNYVLFCHEKAKEEDHSQHCTDMQIIPAQWGLVTVLTFDGYGKERLTNYAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L7W9</accession>
    <name>A0AAR2L7W9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CaM kinase-like vesicle-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Does not appear to have detectable kinase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with calmodulin, in the presence of calcium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L7W9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082063.1">
        <property type="protein sequence ID" value="ENSPNAP00000072650.1"/>
        <property type="gene ID" value="ENSPNAG00000006832.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156468"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000188">
        <property type="entry name" value="CaM kinase-like vesicle-associated, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000155">
        <property type="entry name" value="CaM kinase-like vesicle-associated, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="36"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="513" mass="59244" checksum="295C8CD97043C91E" modified="2024-11-27" version="1">LSSLSLSPLSLRMPFGCLTLGEKKDYNSPTEITDRYDLGQIVKSEEFCEIFRAKDKTTSKMYTCKKFLKKDGRKVRKAAKNEIFILKMVKHPNILQLVDVYETRKEYFLFLELATGREVFDWILDQGYYSEKDTSNVIRQVMEAVAYLHSLNIVHRNLKLENLVYYNRLKHSKIVISDFHLAKLENGLIKEPCGTPEYLAPEVVGRQRYGRPVDCWALGVIMYILLSGNPPFYDEADDDDYENHDKNLFRKILAGDYEFDSPYWDDISDSAKSLVSRLMEVDQDQRLTAQEAINHEWISGNAASDKNIKENVCAQIEKNFAKAKWKVVIMLEMTFFIRHRLVGFVLYFYHCALRISLPARTFGEYSPFFSEGNGSQQGHYLSHSSFADFFSVLEKLPCLSWPCCEVRTQNFVVFEPLLGQSHLDIGVSDMAALIIIVNNVLLSFLFCLLFFLPVPVDKTSEKLKSCEYVLKYHDPFHIALPHISECAFNKSLENIIWVNPALHPKEDTVMKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJA8</accession>
    <name>A0AAR2LJA8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Acid-sensing ion channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Amiloride-sensitive cation channel 2, neuronal</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Forms voltage-independent, pH-gated trimeric sodium channels that act as postsynaptic excitatory receptors in the nervous system, playing a crucial role in regulating synaptic plasticity, learning, and memory. Upon extracellular pH drop this channel elicits transient, fast activating, and completely desensitizing inward currents. Displays high selectivity for sodium ions but can also permit the permeation of other cations. Regulates more or less directly intracellular calcium concentration and CaMKII phosphorylation, and thereby the density of dendritic spines. Modulates neuronal activity in the circuits underlying innate fear.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Li(+)(in) = Li(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78551"/>
            <dbReference type="ChEBI" id="CHEBI:49713"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJA8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087119.1">
        <property type="protein sequence ID" value="ENSPNAP00000076638.1"/>
        <property type="gene ID" value="ENSPNAG00000013947.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158414"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.470.10:FF:000001">
        <property type="entry name" value="Acid-sensing (proton-gated) ion channel family member 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.820:FF:000001">
        <property type="entry name" value="acid-sensing ion channel 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3590.10:FF:000002">
        <property type="entry name" value="acid-sensing ion channel 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.770:FF:000001">
        <property type="entry name" value="Acid-sensing ion channel subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3590.10">
        <property type="entry name" value="acid-sensing ion channel 1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004724">
        <property type="entry name" value="ENaC_chordates"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020903">
        <property type="entry name" value="ENaC_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00859">
        <property type="entry name" value="ENaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF170">
        <property type="entry name" value="ACID-SENSING ION CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01206">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00038517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00044635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00060249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00069596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00077346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="533" mass="60359" checksum="4A1F057C7A7C217D" modified="2024-11-27" version="1">MKPATVMDLKVEPVPVDEPPPLQVFAHMSTLHGIAHIFSYDRITARCCLWLLFFLSSVAFLLCVCVDRVLFYLEYPHVTKLDEVVAPAMVFPAVTICNLNAFRFGRVTRNDLYHAGELLALLNSRYEIRDVHLIEESVLEALKTKADFQNFKPRPFNMLEFYNRTGHDIKDMLLACQFRGSPCQPEDFKVVFTRYGKCYTFNSGETGPLLVSMKGGMGSGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQVHTQDEPPFIDQLGFGVAPGFQTFVSCQEQRLMYLPPPWGNCKASPLDSDFFTSYSISACRIDCETRYLVENCNCRMVHMPGDAPYCTPELYKECADPALDFLVERDNDYCMCETPCNTTRYSKELSFVKIPSKASAKYLAKKFSKSEKYIADNILVLDVFFEALNYETIEQRKAYEVAGLLGDIGGQMGLFIGASILTILELFDYLYEVAKYKLCRCSDKKNQSSSADHSTVLGLDDVKCHWASEKQTHSEMTVNWLCNGKARGQLERCVCFFVFSGGVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLB0</accession>
    <name>A0AAR2LLB0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein disulfide-isomerase A6</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PDIA6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May function as a chaperone that inhibits aggregation of misfolded proteins. Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling. May also regulate the UPR via the EIF2AK3 UPR sensor. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGGT1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with MICA on the surface of tumor cells, leading to MICA disulfide bond reduction which is required for its release from tumor cells. Interacts with ITGB3 following platelet stimulation. Interacts with ERN1; the interaction is direct. Interacts with EIF2AK3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLB0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054013.1">
        <property type="protein sequence ID" value="ENSPNAP00000075452.1"/>
        <property type="gene ID" value="ENSPNAG00000021334.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155646"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02983">
        <property type="entry name" value="P5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03001">
        <property type="entry name" value="PDI_a_P5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000032">
        <property type="entry name" value="Protein disulfide-isomerase A6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000050">
        <property type="entry name" value="protein disulfide-isomerase A6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057305">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24541">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="11"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="16">
        <location>
            <begin position="164"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="146"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="450" mass="49406" checksum="763E86BC66ED3D90" modified="2024-11-27" version="1">MQNYSERDEIRKTCVLVCSFTVLSVHGMYSASDDVIELNPSNFNREVLQSDSLWLVEFYAPWCGHCQNLVPDWKKAATALKGIVKVGAVDADQHKSLGGQYGVRGFPTIKIFGANKHKPEEFQGGRSSQAIVDAALNSLRSIVKDRLSGKTGGSDHSRQSSGGSSGGNKNDVIELTDDNFDQMVLNSDDVWLVEFFAPWCGHCKNLEPEWTAAASQVKEQTNGRVKLGTVDATVHQGLASRYGIRGFPTIKIFRKGEEPEDYQGGRTRSDLVARALDLYSDNAPPPELLEILNQDILKKTCEDHQLCVIAVLPHILDTGAAGRNGYLEVMMKMAEKYKKKMWGWLWTEAGAQMELESSLGIGGFGYPAMAAINSRKMKFALLKGSFSETGIHEFLRDLSVGRGSTSTVGGGALPKIHTVEAWDGKDGVLPVEDDIDLSDVDLDDLEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LTE4</accession>
    <name>A0AAR2LTE4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA/RNA non-specific endonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LTE4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071719.1">
        <property type="protein sequence ID" value="ENSPNAP00000079873.1"/>
        <property type="gene ID" value="ENSPNAG00000030529.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234592"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039015">
        <property type="entry name" value="ENDOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN ENDOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472:SF30">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043557583" description="DNA/RNA non-specific endonuclease domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="3">
        <location>
            <begin position="67"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="2">
        <location>
            <begin position="68"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="29367" checksum="C9CD6E652C071F64" modified="2024-11-27" version="1">MKMWLQHPLILLLAFSSLASCRLVDDFLATPPNGCPQFFLNTPNGHVTPTILQPAQRYQRICQEYLGLDRFATLYDTQNRIPVYSAYLHVPHTQIDTMRYQCLDYLNSGMDKGHLYPSYHNNDVEGMDATFSLTNAAPQARSFRTQWFGQVEYPANELIQIDCGSNTAYVVTGVVPDRILTISNNRVRVASHFWTAYCCLDNSGNVIGHGAFLMDQYGDMLLKPNQNVQDLENLLKLKMIVIHVEKDVKQRYNCIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LXJ2</accession>
    <name>A0AAR2LXJ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540268.1">
        <property type="nucleotide sequence ID" value="XM_017684779.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017540269.1">
        <property type="nucleotide sequence ID" value="XM_017684780.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LXJ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075768.1">
        <property type="protein sequence ID" value="ENSPNAP00000079041.1"/>
        <property type="gene ID" value="ENSPNAG00000035582.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108412656"/>
    <dbReference type="GeneTree" id="ENSGT00950000182890"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000839">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF100">
        <property type="entry name" value="ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="455" mass="52925" checksum="86639CECB1FCFA75" modified="2024-11-27" version="1">MASRRSSSTLQMVSGSLHINASCRRMQKSTAKVRTSHCLECGKNFRTRRILKIHQRIHTGEKPHVCSECGKCFKDTGTLKTHQRIHTGEKPYVCSECGRSFTQLGSLLSHQRIHTGEKPYRCSECGKSFNRQTHLQSHQRVHTGEKPYYCSECGKSFKDRGNFKTHERIHTGEKPYVCSDCGRSFTAQSTFQKHQRIHTEEKPYICSECGRSYKDGGALKTHQRIHTGEKPYHCSYCGRSFTRQSNFISHYRLHTEEKMFHCSECGKRFSRQSNLQTHQRIHTGEKPFYCSECGMHFSLRNSLKRHQRIHTGEKPYVCSDCGKSFTQHSDLQKHQHIHTGEKPYHCSQCGKSFTQQSHFQSHRRIHTGEKPFHCSDCGKSFSLRNNLRKHQRVHTGEKPYVCSECGRSFTQHSSLQKHQRTHTGEKPYHCSQCEKSFTQQSHLRSHKRIHAREKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LXT6</accession>
    <name>A0AAR2LXT6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type phospholipid transporter</fullName>
            <ecNumber evidence="2">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047691.1">
        <property type="protein sequence ID" value="ENSPNAP00000079141.1"/>
        <property type="gene ID" value="ENSPNAG00000022691.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155624"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000232">
        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000264">
        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF190">
        <property type="entry name" value="RETINAL-SPECIFIC PHOSPHOLIPID-TRANSPORTING ATPASE ABCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="655"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="695"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="731"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="760"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1401"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1709"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1761"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1790"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1820"/>
            <end position="1845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1906"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="976"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1969"/>
            <end position="2201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="883"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2296"/>
            <end position="2316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="892"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="927"/>
            <end position="956"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2316" mass="262037" checksum="57ECB3B577E78BB5" modified="2024-11-27" version="1">MNTAGQIRLLLWKNWTLRKRQKTRFVVEILWPVLLFAGLVWLRRANPLYRQHQCHFPNKALPSAGILPWIQGIFCNANNPCFRYPTPAESPGIVSNYNNSILARFYADSQELLFKDTEFQQLGRLWQEASTLSSFMDTLRKDPGRAAGRGLKVEDILKDDETLTSFLLRDAGLSDPVVYQLTKALVRVEQFAYGVPDLSLKEIACSQALLERFIIFPHRRGLYSVRNAMCALSQQRLQDIEDKLYANVDFFKLFKLLPMVLDSHSSGIDLHFWGRVLTAVSDKLEELLSRQSSRDLLKVLVPVFQDGGPSSFSQLTSIISGLFCGYPDGGGSQVLSFNWYEDNNYKAFLGINTSRSHESYVYDESATPFCNALMQNLESNPVTKIVWSSVKPLLMGKILYTPDSPTVHKILKSANTTFEELERLMNILKVWEEVGPQLWQFFQDSVQMNMIRDTLRNPTVMDFLDQRLEGSQFNTKHVLNFLHNGCDTERYENMPRFDWRNVFNLTDQVVRMFIQYSECINLDKFVGHMDEDQLSHQALYLLEENKFWAGLVFMDMYPWTTVLPKHIRYKIRMDIDAVERTNKIKDRYWDPGPRADPMEDLRYIWGGFAYLQDMIEHGILKLQTGQDWPMGVYVQQMPYPCYVDDMFMLTLNRCFPIFMVLAWIYSVSMMVKSIVLEKEMRLKETLKAMGVTNGVIWYTWFIDSFIMMTISTVLLTAIVMAGKVLNYSNPVILFLFLMTFSTATIMQSFLMSVFFNKANLAAACSGIIYFTLYLPHILCFAWQDRITKNMKLASLLSPVAFGFGTEYLSRYEEQGLGLQWDNIGTSPLEGDGYSFLNSIRMMLFDSVLYAVLAWYLDNVFPGQYGIGRPFYFPLQPSYWQNPSPFPSAAPEKPPEENVENMEKVEEKEDRESPAAEPDCNGSRKQCKHQDRRERLEKEKEEQLKREGDLPAPEEERAELTGNLFFEPEPEGLLVGVSVKDLVKIYPGSSRPAVNCLNITFYEGQITSFLGHNGAGKTTTLSILTGLFPPTSGTAYVNGKDIRTDMDAIRQSLGMCPQYNILFNHLTVEEHILFYSLLKGREQKEAEQEVEDMLEDLGLPNKRDDEAQKLSGGMQRKLSVAMAFVGGSKVVILDEPTSGVDPYSRRSIWDLLLKYRTGRTVILSTHHMDEADLLSDRVAIISNGQLHCCGSPLFLKNCFGVGFYLTLVRRIKDLRKKENECDCTSECSCTCSTCTKYKEECQGQYLERALDGNVENITSLIHHHVPEAKLIEVIGQEMTYLLPNKGFKYRSYASLFRELEETLGDIGLSSFGISDTSLEEIFLKVTADGEAANGAVNPENVLLLFAAEANGLQENGVGKHEELDSNAGKASRQVKGFSLVLKQFHALLVKRFHHATRSHKDFLAQIVLPASFVLIALIFTMIVPPFGEYPSLTLSPWIYGQQFTFISNERPDHPKMSHFVHMMLREPGMGTRCMVDLPLENLPCSNITTDWAEPPVSPVVSNILLSPEWTERNPSPSCQCSTEKKLIMLPVCPIGAGGLPPRQRKEATGDILLDLTDRNISDYLVKTYPSLIRTSLKSKYWVNEQRYGGLSVGGQLPILDVDPKQIQNVLSQIGEMMNITGGPRSKLALREIGPFLRYMESEYNVKVWFNNKGWHAMVSFMNIANNAILRANLPHFTNPSEYGITAINHPLNLTKEQLSEVTVLTTSVDAVVAICVIFAMSFIPASFVLYLIQERVTKAKHLQFVSGVSPLVYWVANFFWDMVNYSISSAMVVGIFVAFDKKCYTSPTNLPALVALLCLYGWSVTPMMYPMSYIFSIPSTAYVSLSCINLFIGINSSAITFILELFENNRSLLMFNEGLKKVLLIFPHFCLGRGLIDLAMNQAVTDVYARFGEYIEYAMDPFRWDFVGKYLACMAVEGFVYFILNLLIQYRFFLNHWLSDYSSVPAHDEDDDVAQERQRIYSGGSKNDILHIRDLSKTYVGRKRPAVDRVCVGVPAGECFGLLGVNGAGKTTTFKMLTGDTDVTFGEASVAGYSILTNILDVHQNMGYCPQFDAVDELLTGREHLYLYARLRGVPEREISRVAEWGIQKLGLSEYAGRCAGTYSGGNKRKLSTAIAMIGCPPLVLLDEPTTGMDPHSRRFLWNAIMSIIRDGRAVVLTSHSMEECEALCTRLAIMVNGTFKCLGTIQHLKYKFGDGYVVTMKIRAAKAGASPDLGPAEAFMVSSFPGCIQREKHYNTLQYEIRASSLARVFQLVAANKDKLNVEDYSVSQTTLDQVFVNFAKQQSGEDDDVVLHPRAAGARREMKISPLTATSKSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M4A6</accession>
    <name>A0AAR2M4A6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Refilin A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RFLNA</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with FLNA and FLNB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Refilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037392201.1">
        <property type="nucleotide sequence ID" value="XM_037536304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M4A6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074738.1">
        <property type="protein sequence ID" value="ENSPNAP00000082029.1"/>
        <property type="gene ID" value="ENSPNAG00000036127.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108412767"/>
    <dbReference type="GeneTree" id="ENSGT00390000016836"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031005">
        <property type="term" value="F:filamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061572">
        <property type="term" value="P:actin filament bundle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900158">
        <property type="term" value="P:negative regulation of bone mineralization involved in bone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061182">
        <property type="term" value="P:negative regulation of chondrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048705">
        <property type="term" value="P:skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028215">
        <property type="entry name" value="Refilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31848">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31848:SF0">
        <property type="entry name" value="REFILIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15068">
        <property type="entry name" value="FAM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24880" checksum="ACDD00D16E019F24" modified="2024-11-27" version="1">MVGHLHLQAMDESLKGKNREGLLDSPDSGLPPSPSPPFYSLSPGVVEQRASSCSTPTEPYAHCRKESREGKLLPYLLLNSQGDAKARMYPVVYGESIEVNPKPNKELKFSSAVKYASDRHYRDQVYCAPVPTVTSYRETVVAVRDCTWRSYKSEVHFQPRHRPLHYQSTAIIYPKHARNTYRTTLNYNANGSAGHRWFVSTVRLESSEDASPCIIYTENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M4X6</accession>
    <name>A0AAR2M4X6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ALDH9A1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency (in vitro). Can catalyze the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction, but with low efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-(trimethylamino)butanal + NAD(+) + H2O = 4-(trimethylamino)butanoate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:17985"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16244"/>
            <dbReference type="ChEBI" id="CHEBI:18020"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M4X6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081685.1">
        <property type="protein sequence ID" value="ENSPNAP00000082242.1"/>
        <property type="gene ID" value="ENSPNAG00000030888.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163309"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047105">
        <property type="term" value="F:4-trimethylammoniobutyraldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07090">
        <property type="entry name" value="ALDH_F9_TMBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000019">
        <property type="entry name" value="4-trimethylaminobutyraldehyde dehydrogenase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000016">
        <property type="entry name" value="4-trimethylaminobutyraldehyde dehydrogenase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009725">
        <property type="entry name" value="PRK13252.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="13">
        <location>
            <begin position="54"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="521" mass="56844" checksum="DD0B41F38CEDC35A" modified="2024-11-27" version="1">MLFISFYPLPQFIMAQQPILSLPEFHNVSTGSLVVKEALNFWGGARVQPKDTKNAEPVYEPATGRVLCDMIPCGEVEVDEAIKSAHSAYLKWSKLSGLERARIMLEAARIIRERRDGIARLEVINNGKSITEAEVDIDVAWQCIEYYAGIAPTLAGQHIQLPGGSFAYTRREPLGVCVGIGAWNYPFQIAAWKSAPAFACGNAMVFKPSPMTPLTAVILAEIYKEAGVPDGLFNVVQGGAETGTLLCHHPMVAKVSFTGSVPTGKKVMEMSAKSVKQVTLELGGKSPLIIFKDCELENAVKGALMANFLTQGEVCCNGTRVFVQREIMKPFVEEVVKRTKAIVIGDPLLEGTRMGALISKPHMEKVLGFISQAQQQGAKVLCGGEPYVPSDPKLKGGYYVSPCVLDNCRDDMTCVKEEIFGPVMSILPFDTEEEVLQRANNTTFGLASGVFTRDIARAHRVAENLQAGTCFINNYNVSPVEVPFGGYKMSGFGRENGVVTIEYYSQLKTVVVEMGDVESLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BKE0</accession>
    <name>A0A3B4BKE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD repeat-containing protein 74</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">WDR74</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Isoform 1 interacts (through WDR repeats) with NVL; the interaction is independent of RNA or pre-60S ribosome particles. Isoform 2 does not interact with NVL. Interacts with MTREX; the interaction dissociation in a late stage of rRNA synthesis is required for appropriate maturation of pre-60S particles and depends on the ATPase activity of NVL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BKE0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014144.2">
        <property type="protein sequence ID" value="ENSPNAP00000000277.2"/>
        <property type="gene ID" value="ENSPNAG00000007402.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015119"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22857">
        <property type="entry name" value="WDR74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000214">
        <property type="entry name" value="WD repeat-containing protein 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000287">
        <property type="entry name" value="WD repeat-containing protein 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037379">
        <property type="entry name" value="WDR74/Nsa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038">
        <property type="entry name" value="NOP SEVEN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="334"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000277.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="383" mass="42548" checksum="4D32BAE093717624" modified="2024-11-27" version="2">MADKSQTCSVWLGSETGILKGVSLSKKRAFNFCDMSSVSRDQEICALTWGDRQESEVLVGCVNGSVKTFSTEKGVFTETRVCGDISQGRFTGIAVTDRSLITCVESGLLKVWTEGSADTVEINVGGEVCRMRQNPSQRHRVATGGKENPLKVWDLERPDKPIFTAKNVANDWLDLRVPVWVRDVAFIPDSDRIVTCTGYHQVRVYDPSTPQRRPVLEAEFGEYPLTTLALPAGHDRVVVGNTHGQIAILDLRKGLVRGCMKGLAGSVRGLQCHPSSPLLASCGLDRFLRIHSLEDRSTVRLAPPILFSALLNVFFFSFLLKLVGSDAAGEVEEVKEEERDEVWDSMETVSEKGEKRTVEEEAEESRETAEKKEEKKKRKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNL4</accession>
    <name>A0A3B4BNL4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 410</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CFAP410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in cilia formation and/or maintenance. Plays a role in the regulation of cell morphology and cytoskeletal organization. Involved in DNA damage repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Found in a complex with CFAP410, NEK1 and SPATA7. Interacts with NEK1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNL4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001168"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012612.2">
        <property type="protein sequence ID" value="ENSPNAP00000001168.1"/>
        <property type="gene ID" value="ENSPNAG00000008005.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018807"/>
    <dbReference type="OMA" id="ISICHEL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097733">
        <property type="term" value="C:photoreceptor cell cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000094">
        <property type="entry name" value="protein C21orf2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057125">
        <property type="entry name" value="NXF1/2/3/5-like_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24048">
        <property type="entry name" value="LRR_NXF1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NXF1/2/3/5-like leucine-rich repeat" evidence="7">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="253" mass="28408" checksum="59A167F542193440" modified="2018-12-05" version="1">MKLTRKLVLARAKASDLDSVKKLNCWYVVHISIFSEIPNIEVLTLSANKISSLEHISNCQNLTELYLRRNDVQNLSELGHLKNLACLKVLWLAENPCCEPDPVKYRLTVLRNLPGLHKLDNQVVTEEELALALEQGEEIVTPPGPISAISTNGLAEADSENDPLNYSMEETNKIRAQLGMKPIPRDKFPSFSSPREPGASPKKKSHSLEAVLLLLKDLDVEELKIVQSATENKLRARSRQKEKEKEKALITQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQ60</accession>
    <name>A0A3B4BQ60_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Structural maintenance of chromosomes protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SMC6</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555420.1">
        <property type="nucleotide sequence ID" value="XM_017699931.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQ60"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000651"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013493.2">
        <property type="protein sequence ID" value="ENSPNAP00000000651.1"/>
        <property type="gene ID" value="ENSPNAG00000000376.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428709"/>
    <dbReference type="CTD" id="79677"/>
    <dbReference type="GeneTree" id="ENSGT00550000074816"/>
    <dbReference type="OMA" id="FMCHRSL"/>
    <dbReference type="OrthoDB" id="10072614at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000959">
        <property type="entry name" value="structural maintenance of chromosomes protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF8">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6 ISOFORM X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="16">
        <location>
            <begin position="68"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1071"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="301"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="700"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1100" mass="127257" checksum="9AF0791B8CAC73D9" modified="2018-12-05" version="1">MQGNGFEHICTMNKRKSNVQSLPKPSKICRTEDTTHVQDEDDFDEDGGLHQEHSFSTSSAGDIGVIESITLKNFMSHHLLGPFQFGPNVNFIVGNNGSGKSAILTALIVGLGGKATTTNRGASLKSFVKYGESSAEVTVKLRNRGSDAYKGDTYGDCISIEQRITSDGCRTCKIKNKPGHIVSTKKEELTAILDHFNIQVDNPVSILNQEMSKQFLHSKSEADKYKFFMKATLLEQMKRDYIHIKQTKAVTRDQVERQEECLKDLRQLFLQKKEKYESLSALENMKQTLEDLKKKMAWCLVREKEWQVQQLKEEIDKEQNNRAFEEKLLLCQSKVTLAEKKLRDNQQKLERVKEEEERLAEESRKIKEEVKLKSKAQKSQEVVYFRAENKLKQLEKEQCLFQERIHKISNSNGGRNRESEHAQRIKKISALRNQLEKLGTESIELNQEIKEKQQALFKGREEYDKLSMEEKNIQVSLETKLKRKNQLTASRSNRLRRFGDRMPELLESIDKAYAQGRFIKKPVGPIGACISLKDPSLAVAVESCLRSFMKTFCCDNYKDERVLQELMSPHFPRGCRPQIMVCPFTDCVYNVQSRGVHHPEFPSVLDILIINNPVITNCLIDMRSIECILIIRENAVARRLMQGGRPPRNCREVFTAEGDQVFTNRTYTPEREVIVKYLGGDPEAEIRLVQSEVENHQAQLSRFQLHLSSVKQDIQSMEEKLRSVFIACKRNQESINRVKASITELENIEESQTEDISSLEEEAQENEQKIELERKNVKEAQSELEKHNKVLMEIDRKHQDMKNKREQLTDETEQLKEEQVKAAAECSKLEQSLKILEKKLKAHQDNIQAMRAELGLKIEEKEDFEAKAREICPIPQRPDRSAESIDTEIIRLRQKISSQESSHGNQEQVIREYAEAHGNYKNKSGQVRDLKRFIDRLDNIMTDRQARYKTLRRSLSVRCKLYFNNFLIKLHCCGSMMFDHNNETLSISVKPPGQEKDNINDMRSLSGGERSFSTVCFILSLWEITESPFRCLDEFDVYMDMHNRRISLDLLLELSERQHLRQFIFITPQSTSSLPKSPHIKIHQLQDPERESEEALQSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BST9</accession>
    <name>A0A3B4BST9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spermatogenesis associated 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SPATA7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017546219.1">
        <property type="nucleotide sequence ID" value="XM_017690730.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BST9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001549"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011959.2">
        <property type="protein sequence ID" value="ENSPNAP00000001549.1"/>
        <property type="gene ID" value="ENSPNAG00000000811.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423437"/>
    <dbReference type="CTD" id="55812"/>
    <dbReference type="GeneTree" id="ENSGT00390000014113"/>
    <dbReference type="OMA" id="FITEHEW"/>
    <dbReference type="OrthoDB" id="6263678at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120206">
        <property type="term" value="C:photoreceptor distal connecting cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120200">
        <property type="term" value="C:rod photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029357">
        <property type="entry name" value="SPATA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14917:SF4">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14917">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15244">
        <property type="entry name" value="HSD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="62021" checksum="E41C7A0257E64497" modified="2018-12-05" version="1">MGYLAEFPVMDAKRAFCHGSTGNLTNQHIIQDHMMSHYKKIYSAKAVVDCSVPKSMCHSIKYIDQKRREQMKKDMSERSQSVRSLSQRSLRMNTMTRCSSKNTRPTVRGEESAHFDLRGSAMSSPRFNTSFHCKQVAYASQMTASSQNCRTTSEYSYRSPNSRWHQLTPSCITSESQRKFKSFQDPTQKTYSGDVLHKHAHCFTQEKPFTPRTLKSDCKSTLSTYRYYTPASRKGTDEKTSSKFHRQDTYHGSTRIKQGSSAEQDSSQPYNLEHEWSDEEANEFQHYSTANKFRDSDILFSSSRISPEGMRSPIMRKVTAEEEELMYLEFITDVTNEILARGIYSDRVLERVFERHVDMNRHRLDEVKMRHLLDVLQNDLQSPTAAPVMAVEKDRDSFMLSRHKYSSLGQDLTLTNTDDSCLPAYTPFDKDTEELCETPSLHSTPVNCSVSERAGLIRQGDSVDLELDHQEKNAVSPTPSLDSHQMNKVDKHQNHTENDELMQQLDELGRNMAESLSVSETHGSQEGVEQDSTVKLSDDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTC7</accession>
    <name>A0A3B4BTC7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">GPAT3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BTC7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002902"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009665.2">
        <property type="protein sequence ID" value="ENSPNAP00000002902.2"/>
        <property type="gene ID" value="ENSPNAG00000009267.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234574"/>
    <dbReference type="OMA" id="VWMFVVV"/>
    <dbReference type="OrthoDB" id="10051137at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF10">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="17">
        <location>
            <begin position="233"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002902.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="451" mass="50936" checksum="5577313B855D467E" modified="2024-11-27" version="2">MEGTSWDLALVVFEVWLSVVLGLIVLPAMFGVSLGFTDLYVKALVKTLEWATLRIQRGQKEEDAVPAHLPNGIIEKDDGSMEEEIGELRRSHPKSLAGGDFTLCDAFYFCKKGIESIVEDQVTQRFTSEELASWNLLTRTNNNFHYISVRVTIIWGLGVFIRYCILLPLRVTLAVLGLSWLVIGTTLVGLLPHSNVKTWISDLIHVTCYRICARALSARIRYHNKENHPKKGGICVANHTSPIDIVILANDGCYAMVGQIHGGLMGVIQRSMVRSCPHVWFERSEMKDRHAVAKRLKDHVADKTKQPILIFPEGTCINNTSVMMFKKGSFDIGATIYPVAIKYDPQFGDAFWNSGKYNMVSYLLRMMTSWAIVCNVWYLPPMSLQDGEDAVHFANRVKSAIARQGGLVDLFWDGGLKREKVKESYKEEQQKMYSSMIKGRLPSHERQDCEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWF9</accession>
    <name>A0A3B4BWF9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KIF3B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575650.1">
        <property type="nucleotide sequence ID" value="XM_017720161.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017575651.1">
        <property type="nucleotide sequence ID" value="XM_017720162.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397091.1">
        <property type="nucleotide sequence ID" value="XM_037541194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWF9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002921"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009610.2">
        <property type="protein sequence ID" value="ENSPNAP00000002921.1"/>
        <property type="gene ID" value="ENSPNAG00000009298.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440951"/>
    <dbReference type="CTD" id="9371"/>
    <dbReference type="GeneTree" id="ENSGT00940000153739"/>
    <dbReference type="OMA" id="LESKMLC"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048884">
        <property type="term" value="P:neuromast development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043584">
        <property type="term" value="P:nose development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035845">
        <property type="term" value="P:photoreceptor cell outer segment organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000017">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF76">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="9"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="372"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="719"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="437"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="735"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="747"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="774" mass="88350" checksum="F6D235C0BC42DA3C" modified="2018-12-05" version="1">MSKSKSSESVKVVVRCRPMNEKERAANFERVVSVDVKLGQVAVRNPRGASAHEHPKVFTFDSVYDWNSKQLDLYDETFRPLVDSVLLGFNGTIFAYGQTGTGKTYTMEGVRNDPERRGVIPNSFEHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSRDQSRRLELKERPDTGVYVKDLSSFVTKSVREIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSELGPDGENHIRVGKLNLVDLAGSERQTKTGAQGERLKEATKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNARTVMVANIGPASYNLEETLTTLRYSNRAKNIKNKPRINEDPKDALLREFQEEIARLKEQLEKRSGKKKKKRRRRKFGEAGEELDDDAEEEEDDDDDDDEDGVDGDKNNADYWREQQEKLEKERKAIMEDHSLVAEEKQRLLREKEKKMDDLHREKEALEMLASKVKAMESKLLVGGKNIVDHTNEQQRILEQKRHEIAEQKRREREMQQEMECRDEETLELKETYSSLQQEVDIKTKKLKKLFAKLQAVKAEIHDVQEDHIKERQELEQTQNELTRELKLKHLIIENFIPLEEKNKIVVRATFDEEDDTWKMRPITRIQDDQQMMSRPVSAVGYRRPLSQHARMAMMMRPDARYRAENILMLELDLPSRTTKEYEEPVIAPKVAAALEDALREEDEIQVDASGLYANMGSSPGLGPGAMAGGFSKKPKSGRPKTGKKASTPTAAHSPSGSGSPLYPQSRGLVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BY72</accession>
    <name>A0A3B4BY72_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BY72"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008619.2">
        <property type="protein sequence ID" value="ENSPNAP00000003500.1"/>
        <property type="gene ID" value="ENSPNAG00000009912.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="75" mass="8479" checksum="D94AF2B74ED8C277" modified="2018-12-05" version="1">MNFEPPFFRATIICQYLAVQVALNCIPQLPNNGLPSELKSLFKPSVLLPSQELSTYRKWRKVGAINLSITGKQQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZR0</accession>
    <name>A0A3B4BZR0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma</fullName>
            <ecNumber evidence="9">3.1.4.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 9">Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer composed of two catalytic chains (alpha and beta), and two inhibitory chains (gamma).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the rod/cone cGMP-PDE gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.35" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017563946.1">
        <property type="nucleotide sequence ID" value="XM_017708457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZR0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005212"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005678.2">
        <property type="protein sequence ID" value="ENSPNAP00000005212.1"/>
        <property type="gene ID" value="ENSPNAG00000011493.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433720"/>
    <dbReference type="GeneTree" id="ENSGT00390000013260"/>
    <dbReference type="OMA" id="SHCPQKG"/>
    <dbReference type="OrthoDB" id="8525078at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042622">
        <property type="term" value="C:photoreceptor outer segment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045742">
        <property type="term" value="P:positive regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045745">
        <property type="term" value="P:positive regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1120.10:FF:000002">
        <property type="entry name" value="Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1120.10">
        <property type="entry name" value="Retinal cGMP phosphodiesterase, gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006952">
        <property type="entry name" value="PDE6_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037030">
        <property type="entry name" value="PDE6_gamma_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122">
        <property type="entry name" value="RETINAL CONE RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE GAMMA-SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122:SF5">
        <property type="entry name" value="RETINAL CONE RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04868">
        <property type="entry name" value="PDE6_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000969">
        <property type="entry name" value="35-cGMP_Pdiase_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0140">cGMP</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6" id="KW-0844">Vision</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="87" mass="9023" checksum="C97827B0003903E9" modified="2018-12-05" version="1">MNSAPPAGSALAAPASGAGPTTPKKGPPKFKQRQTRTFKSKAPKPGQKGFGDDIPGMEGLGTDFTVVCPWEAFGDMELSDLAKYGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C107</accession>
    <name>A0A3B4C107_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SRY-box transcription factor 21a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C107"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004349"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007158.2">
        <property type="protein sequence ID" value="ENSPNAP00000004349.2"/>
        <property type="gene ID" value="ENSPNAG00000010802.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160614"/>
    <dbReference type="OMA" id="HEPYSCC"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF313">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004349.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="187" mass="21096" checksum="C663B3C006F072F1" modified="2024-11-27" version="2">MSKPTDHVKRPMNAFMVWSRAQRRKMALDNPKMHNSEISKRLGGEWKLLSDSEKRPFIDEAKRLRAVHMKEHPDYKYRPRRKPKTAARKDRYQLNVAYSLGEPDSLKGALLQGGEAEKAAAVAAATRALLGHPLPTSAYPYVDIHSKMSESAFAHGSSGSFLKRNGGRTRYSGTFPTLLRSSNRVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C1A0</accession>
    <name>A0A3B4C1A0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZFR2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017558071.1">
        <property type="nucleotide sequence ID" value="XM_017702582.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C1A0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004439"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007003.2">
        <property type="protein sequence ID" value="ENSPNAP00000004439.1"/>
        <property type="gene ID" value="ENSPNAG00000010884.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430234"/>
    <dbReference type="CTD" id="23217"/>
    <dbReference type="GeneTree" id="ENSGT00940000162148"/>
    <dbReference type="OMA" id="PMRPWRI"/>
    <dbReference type="OrthoDB" id="8898434at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000001">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000153">
        <property type="entry name" value="Zinc finger RNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000210">
        <property type="entry name" value="Zinc finger RNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000898">
        <property type="entry name" value="zinc finger RNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000010">
        <property type="entry name" value="Zinc finger RNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006561">
        <property type="entry name" value="DZF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049402">
        <property type="entry name" value="DZF_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049401">
        <property type="entry name" value="DZF_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762:SF2">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20965">
        <property type="entry name" value="DZF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
        <property type="entry name" value="DZF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00572">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51703">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DZF" evidence="11">
        <location>
            <begin position="628"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="237"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="413"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="795"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="966"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="434"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="807"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="990"/>
            <end position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1003" mass="110227" checksum="60394D7EAE969F5F" modified="2018-12-05" version="1">MAASHYYGFPHGAAGPQYSTQPPPVYSHQTTASYSVQPAPGVAHAVTASYAPATAQPTRPVASAAYQTYQAHTGQDYGYRQQETAPQPTTTPQSYQVFTEAESYNYGRPVSASAGYENKQYYQASIAPAERTPTESYFQQGLKSGYRPATAAYSQPAPQRQVTSLKPLPPVSSVSTSYNIYSATTSVQQQPTPISTYTPSSSSYNSTAATSYSGLSYSTYESSGYTSTSSYYQPVQQLGPQQTPAQAPSHQPQPPQQQTQPPAKQLTSASWSNTGNSMVTSSPVNTYKKPMFHQNKVQKPKGPPKQPQLHYCDICKISCAGPQTYREHLEGQKHKKKEASQKSGSQASNGLRGVQTQLRCELCDVSCTGADAYAAHIRGAKHQKVVKLHTKLGKPIPSTEPVLVNSAPVPITTTSGKPAAPAVAPASPTVPQQPTAPNPPKGQPPAKKPALTKATVIANKAAAPEPVKVEEVKPAVLLSDPQSDDEGDGGGAQGDIQPVGHDYVEEVRNDDGKVIRFHCKLCECSFNDPNAKDMHLKGRRHRLQYKKKVNPDLMVEIKPSNRARKLLEGKLRKQKQKAVLKRQQEDEHRWHMEMRRYEEDMYWRKAEEEHLYWGDQRRRMAPPPLMSRPGMPVPPLLPVRRPDSPDDRHIMAKHATIYPVEDELQAVQRIVSHSERALKLVSDSLLEQEATTQSKDTVEKPLDPQRRVLKGVMRVGILAKGLLLHGDRNVQLILLAAKKPTVSLLNSIAEQLPKQLTTFSEDQYVVQAHPEEANIVIISSKEPKMQVTISLTSPVMREDPAPSPEEQAGHKLPEKVPEKDPPDVLNKTKCLDYLAALRHAKWFQARANGLQSCVIIIRLLRDLCQRVPTWGKMPAWATELLVEKAISSASGPLSPGEAMRRVLECIASGILLQDGPGLFDPCEKVQTDALGSMTKQAREDVTASAQHALRLLAFRQIHKVLGMDSLPASKASARSRKRRRDGTDVGEGEGEGKKDKKEDSQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C1I5</accession>
    <name>A0A3B4C1I5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tumor protein p53-inducible protein 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">p53-induced gene 11 protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C1I5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006838.2">
        <property type="protein sequence ID" value="ENSPNAP00000004544.2"/>
        <property type="gene ID" value="ENSPNAG00000001479.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017249"/>
    <dbReference type="OMA" id="CYCAMAS"/>
    <dbReference type="OrthoDB" id="6243248at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028266">
        <property type="entry name" value="TP53I11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31584">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31584:SF1">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14936">
        <property type="entry name" value="p53-inducible11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
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    <evidence type="ECO:0000313" key="9">
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            <dbReference type="Ensembl" id="ENSPNAP00000004544.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="217" mass="24394" checksum="A3C4F7D89A971278" modified="2024-11-27" version="2">MLTTLYVSEDKKSNQECQAFKPAALATRMTAKPHPPLMKKHSQTDLVSRLKTRKILGVGGEDDDGEVHRSKISQMLGNEIKFAVREPVGLRIWILFSAVVFTVIALMALAFPNQLYEVVFDQEQTATSVSIRLYGGALLSLSLIMWNGLYTAEKVIIQWTLLSEACYFAIQFLVTSVTLVELGLLSNPAILLLLSRILFLTITLSYYYHLGRRPKKI</sequence>
</entry>
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    <accession>A0A3B4C244</accession>
    <name>A0A3B4C244_PYGNA</name>
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        <name type="scientific">Pygocentrus nattereri</name>
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            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        </lineage>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
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                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSPNAG00000001543.2"/>
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    <dbReference type="OMA" id="WALQTEM"/>
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        <property type="component" value="Chromosome 26"/>
    </dbReference>
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        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
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            <begin position="32"/>
            <end position="52"/>
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    </feature>
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            <begin position="298"/>
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    </feature>
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            <begin position="60"/>
            <end position="118"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4Y0</accession>
    <name>A0A3B4C4Y0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Microtubule-associated protein RP/EB family member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MAPRE2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAPRE family.</text>
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        <property type="nucleotide sequence ID" value="XM_017718297.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000006125"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004089.2">
        <property type="protein sequence ID" value="ENSPNAP00000006125.1"/>
        <property type="gene ID" value="ENSPNAG00000012207.2"/>
    </dbReference>
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    <dbReference type="CTD" id="10982"/>
    <dbReference type="GeneTree" id="ENSGT00490000043329"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="57"/>
            <end position="159"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="240"/>
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        </location>
    </feature>
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            <begin position="206"/>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019567"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="332" mass="37331" checksum="A202208036617ABB" modified="2018-12-05" version="1">MPGPTQALSPNGENNNDIIQDNGTNIIPYRKNTVRGERAYSWGMAVNVYSTSITQETMSRHDITAWVNDLLCLNYTKVEQLSSGAAYCQFMDMLFPGCISLKKVKFQAKLEHEYIHNFKLLQASFKRMNVDKIIPVEKLVKGRFQDNLDFIQWFKKFFDANYDGKEYDPVQARQGQDAIPPPDPGEQIFNLPKKSHHAASSPTAGATRSSTSTPKSSTSTSRPSSAKKIPVMSSTPAKGEKELEAQVAQLNEQLNTLKLALEGVEKERDFYFGKLREVELLCQEQGQDNGPFVERLMEVLYSTDEQEASAEGEEHEALAQEGEVPDDTQDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5L2</accession>
    <name>A0A3B4C5L2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CABIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAREM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570214.1">
        <property type="nucleotide sequence ID" value="XM_017714725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5L2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007157"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002307.2">
        <property type="protein sequence ID" value="ENSPNAP00000007157.1"/>
        <property type="gene ID" value="ENSPNAG00000002533.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437565"/>
    <dbReference type="CTD" id="100331995"/>
    <dbReference type="GeneTree" id="ENSGT00530000063834"/>
    <dbReference type="OMA" id="CLPACAR"/>
    <dbReference type="OrthoDB" id="6077228at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025946">
        <property type="entry name" value="CABIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052281">
        <property type="entry name" value="GAREM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454:SF5">
        <property type="entry name" value="GRB2-ASSOCIATED AND REGULATOR OF MAPK PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454">
        <property type="entry name" value="GRB2-ASSOCIATED AND REGULATOR OF MAPK PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12736">
        <property type="entry name" value="CABIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CABIT" evidence="4">
        <location>
            <begin position="25"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="505"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="651"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="536"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="664"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="803" mass="88230" checksum="3A8D9AB28B93D9AF" modified="2018-12-05" version="1">MELLADLSWSSVALPLDAVVAKFRLPALVRLSQGENVEGLSEEDVVLLHSCRQWTTVTAHSLEEGHYVIGPKIDIPLQYQGKFKLLDQDRDVREPVQYFSSVEEIAGTFPDRVFVMEPITFSMKVVSGEFSEDSEVYNFSLQAGDELTLMGQAELLCVQSPREKSRLAALLRRLGKAGGALGRPTRAKVPCLICMNHRTNESVSLPFACRGRFCTRSPQELRMQSGEHTVRSIVERVRLPVNVAVPTRPPRNPYDQHCVREGQRYKLLGIISKTVVLCCVLRKEEVTPSHFLLLTDMPRFALPDGLLRSDPIYQQAVLQSAIRCQDMFDPDDYSRAVREVKTDFMEECLSPRRIRVCVQGFGRNDLGQSLQRLSLCVYGGGTITHTLTPGCRDSLSNTHTSPSEGEGEDREYVTPDWSTETQEIPYEELWTNQSGGSYGEVQESTVKVEHNLISFHSTSSLDGTAGPAQVATTVQMEAGRVATPPPVPPKSEAVKEECRFLNAPPVPPRCAKVGGPAPSPPVPPRFPKAPPTTTRSPNLSYYSSGLQESSAPRSDSSSPSPDSYSLYCYPCTWADCVTSDPSVSPDPTQPAQACWSHPRGGGAFTSNVLNTPLLSTDSANKNYSTCTRPRPQAQNRFAPFGALNPFANPGHRHSSSDWLVPGRSSPTLIPDLTSSAHKEPSANQETSEASPSPLARPTKSTEEEEAVTPNLASSLATARGAEGGAVSLWRPPADLCWLSVEEVSASLRFIGLSDDVVGLFSRERIDGSIFTQLTEEILSEDFNLTKLQVKKILQFIKGWRPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C717</accession>
    <name>A0A3B4C717_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TTC32</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C717"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007672"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001447.2">
        <property type="protein sequence ID" value="ENSPNAP00000007672.1"/>
        <property type="gene ID" value="ENSPNAG00000007172.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424486"/>
    <dbReference type="CTD" id="130502"/>
    <dbReference type="GeneTree" id="ENSGT00390000011905"/>
    <dbReference type="OMA" id="MEEDNSQ"/>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47059">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47059:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="85"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="140" mass="16596" checksum="4B3B0B958127658E" modified="2018-12-05" version="1">MEDPLLKESTALLSEAHEEFNSGRYKRAEELYTEFISTCTTARECDHNELAIAYNNRGQVKYFRVDFYEAMDDYSEAIKINGRFEVPYYNRGLIRYRLGFFQDAESDFRKALEINANFEDAKQSLHQTMVDREEKLKRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CCD1</accession>
    <name>A0A3B4CCD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">COPS2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563796.1">
        <property type="nucleotide sequence ID" value="XM_017708307.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CCD1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009188"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035229.2">
        <property type="protein sequence ID" value="ENSPNAP00000009188.1"/>
        <property type="gene ID" value="ENSPNAG00000014767.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433621"/>
    <dbReference type="CTD" id="9318"/>
    <dbReference type="GeneTree" id="ENSGT00530000063301"/>
    <dbReference type="OMA" id="SEENWKD"/>
    <dbReference type="OrthoDB" id="194139at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000001">
        <property type="entry name" value="Putative cop9 signalosome complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058796">
        <property type="entry name" value="COPS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25983">
        <property type="entry name" value="COPS2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="254"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="443" mass="51588" checksum="1DB6FA774BE6B227" modified="2018-12-05" version="1">MSDMEDDFMCDDEEDYDLEYSEDSNSEPNVDLENQYYNSKALKEDDPKAALSSFQKVLELEGEKGEWGFKALKQMIKINFKLTNFPEMMNRYKQLLTYIRSAVTRNYSEKSINSILDYISTSKQMDLLQEFYETTLEALKDAKNDRLWFKTNTKLGKLYLEREEYGKLQKILRQLHQSCQTDDGEDDLKKGTQLLEIYALEIQMYTAQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAKPYKNDPEILAMTNLVSAYQNNDITEFEKILKTNHSNIMDDPFIREHIEELLRNIRTQVLIKLIKPYTRIHIPFISKELNIDVADVESLLVQCILDNTINGRIDQVNQLLELDHQKRGGARYTALDKWTNQLNSLNQAIVSKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDQ4</accession>
    <name>A0A3B4CDQ4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CDQ4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009006"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015221.2">
        <property type="protein sequence ID" value="ENSPNAP00000009006.1"/>
        <property type="gene ID" value="ENSPNAG00000014579.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="OMA" id="IFFQYRH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00592">
        <property type="entry name" value="CASENSINGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017272023" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="587"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="625"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="658"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="702"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="747"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="782"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="808"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="588"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="861" mass="95300" checksum="26907A1582C87E3E" modified="2018-12-05" version="1">MLLLADIMMIIFFTADATAQQCMAYGADDLLHFSKEGDVSIGGIFSFHQFPLDVNPTLQVNPGNIRCFGVDPGELQYAMTMMFAIEEINNSTDILPGFTLGYRIYGSCPSIPLSVKASLVLINGPEDRIDECIKLSSVHAVIGETTSTATIGIARTMGPFRIPVLSHSATCACLSNRKEYPSFFRTIPSDYYQSRALAKLVKHFGWTWVGAVRSRSDYGNDGMASFLEAADKEGVCVEYSVAIYRTDPREKFLDVVDVIKTSTSRVIVAFADGNDLDILIEELYYQNVTGFQWVGSEGWITYRYLANAINYAVVGGAVGFAVPNAHIPGLKEFIASSRPYMTPGNTGLVELWENVFDCNLRLQSHNGAKSCTGEESLETAHTRFTDVTDASLLNNIYKAVYAVAYAVDGLLACVEGKGPFPNGSCANTRRIEPWQVLHYLSQVNITMKNGEKVLFDELGDPVARYALVNWQMNSEGSIIFETIGLYDASQQDGQEFVMNDDVTAVWAGDQRMVPRSVCSESCLPGTSRAFTEGKPICCFDCIPCADGEFTNTTNAVTCLPCPLEYKSNGNKTRCDLKNIEFLNFNEVMGILLVTFSLFGGCLTITIGLIFFHYRQTPIVRANNAELSFLLLFSLTLCFLCSLTFIGRPSEWSCMLRHTAFGITFVLCISCVLGKTIVVLMAFRATLPGSNAMKWFGPPQQRLSVLAFTLIQVLICILWLTISPPFPHKNMNHYKDRIILECHVGSAIGFWAVLGYIGFLALLCFILAFLARKLPDNFNEAKFITFSMLIFCAVWITFIPAYVSSPGKFIVAVEIFAILASSSGVLFCIFIPKCYIILLRPDKNTKKHMMGKHPLKSKCLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGS3</accession>
    <name>A0A3B4CGS3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGS3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035564.2">
        <property type="protein sequence ID" value="ENSPNAP00000010066.2"/>
        <property type="gene ID" value="ENSPNAG00000015567.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163148"/>
    <dbReference type="OMA" id="GEYNDMQ"/>
    <dbReference type="OrthoDB" id="8407241at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF139">
        <property type="entry name" value="CRYSTALLIN, GAMMA M1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="3"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="42"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="134"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010066.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="180" mass="21427" checksum="5612FCB4E93C80A6" modified="2024-11-27" version="2">MGKVIVFYEERNFQGRNYECMSDCSDISSDLSRVSSIRVESGCFMVYESNSFRGNQFFLKTGEYYDIQRMLGMGMMFDTIRSCRMIPQHRGAFRMKIYERESFAGQMIELMDDCDSIMDRFRMSDCQSCHVLDGHWLMYEQPHYRGRMIYVRPGEYRSLREVMGIGTMRIMSMRRITDMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CHM7</accession>
    <name>A0A3B4CHM7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CHPF2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus</location>
            <location evidence="1 10">Golgi stack membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_017578767.1">
        <property type="nucleotide sequence ID" value="XM_017723278.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CHM7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017642.2">
        <property type="protein sequence ID" value="ENSPNAP00000010860.2"/>
        <property type="gene ID" value="ENSPNAG00000016493.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442952"/>
    <dbReference type="CTD" id="54480"/>
    <dbReference type="GeneTree" id="ENSGT01050000244857"/>
    <dbReference type="OMA" id="YLNRVRM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050510">
        <property type="term" value="F:N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000004">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF14">
        <property type="entry name" value="CHONDROITIN SULFATE GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043926908" description="Hexosyltransferase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010860.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="764" mass="87903" checksum="68666722D30AFFAF" modified="2024-11-27" version="2">MRLSSFLAVFRPALPLILGLSLGCSLSLLMVSWTQGDTEDGCSDELLNGGLLQGSHVGEHQGANGEDGNEDFQPRIVPYHKDPSKPHKKVLRTRYIHTELGIRERLLVGILSSRTTLNTMGVAVNRTVAHRFHRTFFFTGLRSTKIPHGMMVVAHGDDRPVWLMYETIRHLHQHHGNEYDWFYLAQDDTYTQAERVMDLVGHLSAGQDLYMGRAEEFIGGEERARYCHGGYGYLLSRSLLARLQPHLDSCRNDILSVRPDEWLGRCIIDYLGLSCLEKHQEMTYRYFELQKNADPEREDSSQFKNAFTVHPVSEPTLMYRLHKRYSQIELDWTYAQIQQLQVQISNLSELTPEGKTGTTWPIGVNPPFQPKTRFEVINWEYFTEDHIYSCADSSPKCELRGADRADVNSILETAVERLNERYQPQLRFRKQRLLNGYRRFDPTRGMEYMLDLALEAFTQKGHSQVIAKRVGLLRPLSAIEIIPMPYVTEATRVQVILPVTARDQDFVGNFLDTYVVNALDTRDNALLTFLFVYDPFDAQRVSQTDVFAGVKAMIGEVEKRFSEVKIPWISVKTEVPSQVKLMDIISKKHPVDTLFFLASVWTEVNADFLNRCRMNAISNWQVFFPIHFQEYSPALVYRDQQPSAASSFSSEALRDGRFDRHVFEEACFYNADYMAARTKMAADILDNDEMLESMDVYEIFVRYSGLHVFRAVEPALVQKYVHRVCNPRFSEDIYHRCVLSNLEGLASRSHLAMALFEQEQANST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CHR1</accession>
    <name>A0A3B4CHR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">H1-10</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CHR1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010978"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017806.2">
        <property type="protein sequence ID" value="ENSPNAP00000010978.1"/>
        <property type="gene ID" value="ENSPNAG00000003423.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111536"/>
    <dbReference type="OMA" id="FDQQHGR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="4">
        <location>
            <begin position="53"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="128"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="203" mass="21869" checksum="A33110A50FF70C49" modified="2018-12-05" version="1">MSAQVEETAPAPAPAPAPAAPATKKKATPSKFKAAAKSASPDGKKKKKKKSKGPGKYSQLVIEAIRTLGEKNGSSLFKIYNEAKKVTWFDQQNGRMYLRYSIRALLFNDTLVQVKGLGANGSFKLNKKKFETKSKKRSAKPAKAPKPTKKPAKKAAEKKKKSVKTAKKTTPAAKKAAKPKKAPKKPAVSVKKGTKAKTAKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMV4</accession>
    <name>A0A3B4CMV4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytidine monophosphate-N-acetylneuraminic acid hydroxylase</fullName>
            <ecNumber evidence="4">1.14.18.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CMP-N-acetylneuraminate monooxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">CMP-Neu5Ac hydroxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">CMP-NeuAc hydroxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sialic acids are components of carbohydrate chains of glycoconjugates and are involved in cell-cell recognition and cell-pathogen interactions. Catalyzes the conversion of CMP-N-acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly expressed at the surface of many cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>CMP-N-acetyl-beta-neuraminate + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = CMP-N-glycoloyl-beta-neuraminate + 2 Fe(III)-[cytochrome b5] + H2O</text>
            <dbReference type="Rhea" id="RHEA:16145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:58376"/>
            <dbReference type="EC" id="1.14.18.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-sugar metabolism; N-acetylneuraminate metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CMP-Neu5Ac hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.18.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMV4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012675"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036619.2">
        <property type="protein sequence ID" value="ENSPNAP00000012675.2"/>
        <property type="gene ID" value="ENSPNAG00000003530.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010830"/>
    <dbReference type="OMA" id="ETGMGNN"/>
    <dbReference type="UniPathway" id="UPA00628"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030338">
        <property type="term" value="F:CMP-N-acetylneuraminate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046381">
        <property type="term" value="P:CMP-N-acetylneuraminate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006054">
        <property type="term" value="P:N-acetylneuraminate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027033">
        <property type="entry name" value="Cnh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46522">
        <property type="entry name" value="CYTIDINE MONOPHOSPHATE-N-ACETYLNEURAMINIC ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46522:SF1">
        <property type="entry name" value="INACTIVE CYTIDINE MONOPHOSPHATE-N-ACETYLNEURAMINIC ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="14">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012675.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="594" mass="68409" checksum="7D483639536E39CC" modified="2024-11-27" version="2">MAQVCRTALTLDAPNVRNLKEGINFTKSPDGQCFIIYKHQDTIKACRNQCKHQGGRFIKDIEDMDGRTVRCTKHYWKLNVANMQYVNPPDSFLQDELEVVQSVDGGLKLLELDPPDPWDTDPRPAQELQPGEITLTYITHACMELKAGGKSMVFDPWLTGPAFARGWWLLHEPPKGAMEQLCQADLIYISHMHSDHLSFPTLQLLSEKRPDIPIYVGDTSRPVFWYLNRSGVNLTNINVVPFGIWQNVDEHLRFMILMDGVHPEMDTCLIVEYKGHMILNTVDCTRPNNGHLPRNVEVMMSDFAGGASGFPMTFQKGKYTETWKVDYIKAERRKLLNYKAQLVDSLQPKVYCPFAGYFIEAHPADRYIKDINIKNSPAELNALIKDRCPDIITWTPLPGSTLDLAKALMHHKDAITEPPSGTQVFKDSWHFDVYVDKLNTAISSEIFEHKSWIQVYYNWAGFQDYNLVIRMIETDDDFQPLDGGYDYLVDFLDLSFPSVRPQRDHAYLEIQNRVGVIRHVVLNGLLWDDLYIGFNNRISRHPDIYHHRFWKHFQSELPLSAPDWDSFLRINATFTTVFHQTNCSASPFISTNPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSH3</accession>
    <name>A0A3B4CSH3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PPP4R3C</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CSH3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021359.2">
        <property type="protein sequence ID" value="ENSPNAP00000013696.2"/>
        <property type="gene ID" value="ENSPNAG00000019606.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286927"/>
    <dbReference type="OMA" id="ECESHTI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000100">
        <property type="entry name" value="Si:dkey-11n14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="75"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013696.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="824" mass="93904" checksum="B2A22E1DD13C946C" modified="2024-11-27" version="2">MKFQKINEGFFKQGHTTHLNEIYIELYITEGGSGEVNNEHEVRQIEISRRQPTEETPIQCNDIFKSSPEQDKVIRTVLTRGVAGIGKTVSVQKFILDWIDGIANQDITFIFPLPFREMNLMKGKKLSLINLLQCIFPEIEILTLIDYVKNKVIFILDGLDEYRLPLDFQNNESLFDVRESTSVDVLLTNLIKGNLLPSALLWITTRPAAANQIPSECVDQVTEIRGFSDPQKDEYFRKRISDQSIAEKIITHIQSSRSLYIMCHIPVFCWISATVLEKMLGETESREIPKTLTQLFTQFMVFQTKHGSQKYQGTCVIDPHQTRESILALGKLAFQQLQKGNLIFYEEDIRECGINVREMSVHSGMYTQIFREEFELDQVFTFVHLSIQEFFAALYAFLSFILNNENILKQKNGHLDLFLRFLLGLSLETNQTLLRGLLPQTATSSHRTNEIIKYIKRKIRENPSPEKSINLFHCLNELNDHSLVQEVQTYLNTRVYGRLSGAKLSPAQWSALVFVLLNSEEELDEFNLRKYNKSDECLLRLLPVVKASRKAELCECNLTEESCVTLASALSSNSNLRKLDLSKNNLKDSGVKLLSSSLKSPECKLQILRLEFCNITEAGCWALVKALKSNPSNLRELNLKYNKLGEEGIKKIHRFKCFTVFINSLCNPLHLQELDLGGNKLGHSGVKQLSHLLEDPQCKLEKLQLVNCDLTEESCAALASVLGSESSNLKHLILSFNQLEDSGVKLLSAGLENPHCKLETLRWVHYFLTTDANLPHRNAHKRVVFVENILTRLVHCVNTVIIWGLTIMVGAKLGPSLKISYHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVE9</accession>
    <name>A0A3B4CVE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">E3 ubiquitin-protein ligase TRIM71</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein lin-41 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase TRIM71</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Tripartite motif-containing protein 71</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">TRIM71</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and participates in post-transcriptional repression of transcripts. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017555898.1">
        <property type="nucleotide sequence ID" value="XM_017700409.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVE9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015365"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023530.2">
        <property type="protein sequence ID" value="ENSPNAP00000015365.1"/>
        <property type="gene ID" value="ENSPNAG00000021404.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428989"/>
    <dbReference type="CTD" id="131405"/>
    <dbReference type="GeneTree" id="ENSGT00940000159099"/>
    <dbReference type="OMA" id="WKQFDSP"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19812">
        <property type="entry name" value="Bbox1_TRIM71_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19796">
        <property type="entry name" value="Bbox2_TRIM71_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14954">
        <property type="entry name" value="NHL_TRIM71_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000013">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000025">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000080">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000527">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000923">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000435">
        <property type="entry name" value="Tripartite motif containing 71, E3 ubiquitin protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF55">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="16" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="25">
        <location>
            <begin position="12"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="26">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="23">
        <location>
            <begin position="423"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="537"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="584"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="631"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="678"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="725"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="775"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00059249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="812" mass="89258" checksum="FCE890D22D2A7031" modified="2018-12-05" version="1">MASFPDSDLQTCPLCKELCGSSAPISSSSSTSSSSSQTSNCSTSSTRRLHVLPCLHAFCRQCLEGLRNNGDPLKLRCPTCDQKVAMSESGVDALPSSTFLFSDLLDVVVSAEEQGKGNNCRGNGNPRGGLLRAHHLADPQCSSCDEGNPATSHCLDCREYLCDNCVRAHQRVRLTKDHLIERRMESLHLAGRANSSSTPLSLSQSFHNFSLLPVFHDRVDYCQQHDNAVLRFFCESCSMPICRECSAGRHVGHSVSYLQDALHDSRALTIQLLADAQQGRQAVQLSIEKAQAIAEQVELKAKVVQSEVKTTTLRHKKALEERECELLWKLEKIRQVKAKSLYLQVEKLHQNLTKLDSTVATVTKVLEEGRSMDILLAREHMLNQLHELKALRCILQPQEDDRIMFTPPDQALLIAIKSMGLISSGAFASATKAHGEGLKRALQGKLTSFTVVGYDHDGEPRLSGGDGVSVVLMSPDGNLSSADVSDHQDGTYTVSYLPKSEGEHLLSVLICNQHIEGSPFKVMVKSGRSYGGIGLPVASFGGEGDGDGQLCRPWGICVDKEGYVIVADRSNNRIQIFKPCGTFHHKFGTLGSRPGQFDRPAGVACDSQRRIIVADKDNHRIQIFTFDGQFLLKFGEKGTKNGQFNYPWDVAVNLEGKILVSDTRNHRVQLFGPDGVFLNKYGFEGALWKHFDSPRGVAFNQEGHLVVTDFNNHRLLVIRPDCQSARFLGSEGTGNGQFLRPQGVAVDQEDRIIVADSRNHRIQVFEPNGNFLCMFGTQGSGFGQMDRPSGIAVTPDGMIVAVDFGNNRILMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVT5</accession>
    <name>A0A3B4CVT5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVT5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022888.2">
        <property type="protein sequence ID" value="ENSPNAP00000014871.2"/>
        <property type="gene ID" value="ENSPNAG00000030799.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163587"/>
    <dbReference type="OMA" id="AVWNWIC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRY"/>
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    </dbReference>
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        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPRY"/>
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        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
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        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="203"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014871.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="406" mass="47108" checksum="0E2844F5985FFFEB" modified="2024-11-27" version="2">MIKECLQFLIEPAKNLKIRLKESRRRVQSERREPLTESQLFIMELARELTWICQRSDVLAHIWTGEDIWPPGVCKEFIVETASILEMKEKSERPLLTRSGPSMEKQDWRERLLSVLEGEENDLSDSKRTIMDWTHSQRTLHPHGIWPGEPVLLMLGDLELQWKRGRLRHLQAAMELLIWVALGEHLDKELVPRLWLVQKQKSQNVGNVTCSPALHFCSSAVEVTLDPDTAHPALLVLDDGKRMRCIPEPLDVPRHRQRFDGWICAVGTEGFSCGRRYWEVEVGDRDWRLGVARESALRRGYDSLNTHSGYYTLRLERGTELKALTLPVTPLTPAPMPRWVGVYLDYEEGQLSFYDIQRRSHIYTFCERFTETLFPIFGTAEMVRDMVVRPAGLRWPCFCNGPCLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CW87</accession>
    <name>A0A3B4CW87_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DnaJ homolog subfamily C member 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DNAJC16</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CW87"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016362"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024819.2">
        <property type="protein sequence ID" value="ENSPNAP00000016362.2"/>
        <property type="gene ID" value="ENSPNAG00000022574.2"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10065037at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02963">
        <property type="entry name" value="TRX_DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_C16_autophagy_reg"/>
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    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
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        <property type="entry name" value="DNAJC16_TRX"/>
    </dbReference>
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        <property type="entry name" value="J_dom_sf"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303:SF3">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ"/>
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        <property type="entry name" value="Thioredoxin"/>
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    </dbReference>
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        <property type="entry name" value="JDOMAIN"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
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    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043568258" description="DnaJ homolog subfamily C member 16" evidence="3">
        <location>
            <begin position="28"/>
            <end position="788"/>
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        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
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        <location>
            <begin position="31"/>
            <end position="95"/>
        </location>
    </feature>
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        <location>
            <begin position="115"/>
            <end position="253"/>
        </location>
    </feature>
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            <end position="590"/>
        </location>
    </feature>
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        <location>
            <begin position="714"/>
            <end position="748"/>
        </location>
    </feature>
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        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
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            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="788" mass="90670" checksum="E36A3D2B90AA06FE" modified="2024-11-27" version="2">MAGRAMLFSRRSLLLFCALLAAALGDAAHFDPYKILGVTRSASQPEIKKVYKRLAKEWHPDKNKNPEAEDMFIKITKSYEILSSEEKRANYDRYGQTDDTQPYGHRGFRHFHDNFYFDESFFNFPFNSKSGRDSADSKYTLHFNQYVNEVVPDSFKRPYLVKITSDWCFSCIHIEPVWKEAVQELEPLGVGIGVVDVGYERRLANHLGAHRTPSILGVINGKVTFFHYAVVKEHLMQFVEDLLPQRLVEKVTDRNDRDFLKSWHEVNKPHVLLFDQVPVVPLLYKLTAFAYKDYVQFGYVDQGLSETANLLKQYNINTYAPTMLVFKENTDKPADIIQAKGMKKQIIDEFISNNKFLLAPRLVNQKVFDELCPVKQFHRRRKYCVLLITGEEEAFVIGNKAFLSLASANTNEVLRFAYVYQRQQQPLCTVLLKNRDSTPPQVVILERRNGAGKVLYKPVLGGWNGSEEDKHRLLEELERLQKDPSILTHDATLPELNNEFASMFLIRWIYTAYDYLSEIIDDLLHNNWREMMPLLSLIFSALFILFGTVVIQAFSDSSDEKQSKPKAKEAAKTENGSPNTTNTSSSRPPKKNFVEVTELTDITYTSNLVKLRPGHINVVLVLTDTTKNILLSKFAKEVYSFTGSLTLHFSFLNVDKHNEWMATLLEYAQDAMQIDMDEEEGSNRKIDYTGYVLALNGHKKYLCLFKPVYTGEDADGKSEDDTGSSRSRSGSKDEHQHRKSTTRSRSTTLQIHHKLDRLGLWMERLMEGTLPRYYIPAWPGLDKITVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYN1</accession>
    <name>A0A3B4CYN1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RAB, member RAS oncogene family-like 6b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017553814.1">
        <property type="nucleotide sequence ID" value="XM_017698325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYN1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024261.2">
        <property type="protein sequence ID" value="ENSPNAP00000015906.1"/>
        <property type="gene ID" value="ENSPNAG00000022005.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427817"/>
    <dbReference type="CTD" id="572091"/>
    <dbReference type="GeneTree" id="ENSGT00390000016002"/>
    <dbReference type="OMA" id="RNAHETD"/>
    <dbReference type="OrthoDB" id="207081at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="RABL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14932:SF1">
        <property type="entry name" value="RAB-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14932">
        <property type="entry name" value="RAS GTPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="644"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="498"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="694"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="739" mass="81408" checksum="5E2E9DEFA944FECD" modified="2018-12-05" version="1">MFTALKKLVGSEAGQFRDKNIPAGLQSMNQSLQRRFAKGVQYNMKIVIRGDRNTGKSALWHRLQGKKFIEEYIPTQEIQVTSIHWNYKTTDDVVKVEVWDVVDKGKGKRRGENLKLENEPQESETEMALDAEFLDVYKNCNGVIMMFDITKQWTFNYILRELPKVPTHVPVCVLGNQRDMGEHRVILPDDTRDFINSLNRPPGSSYIHYAESSMKNGFGLKYLHRFFNIPFLQLQRETLLRQLETNQLDIDATLEELNVQQETEDQNYDIFMEMMEARKAYGSPAPSNSQSPSSGSQSPIVLPGAASPSNSSPSTPQAPLPAPPQPHPLPDTHPAHLPLQSSAPPVPKNHPPSQTSEPATATHVAQAAPPAQKRGFISRLFGSSTPESPDKPETAAFEALKPSGKVQNVDDFVPDVGLDRGFLEDSGEANKSKKKAPAAAPAQDSDSDAEGRGGNPLVSGFQDDLDPDDQEPSRPMIKATAPSLGITLTSDEEDEKPTSTAAHNKGLSTGFDTKGFTVNSVKQQSTKSKPTERPHSLKTSSHSPGQKHGHKAGGTVEAESSDSDDEGPVAHQILSFVMDDPGFDSNELGVTVKQKSQSFPARDDISEKSDDGFVLAGLVDPPKITPSLFKSLNSDIDLFDLGIEESTPKGKDSSEDQDDIDSRHFSKDKKKKKKKDKEEDDKSSKKRHKHKKKDREESGTSDEKEKKKKKSKSKKSDNVGDLEAFLAGGKSEGGDYEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZD6</accession>
    <name>A0A3B4CZD6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 33B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZD6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024570.2">
        <property type="protein sequence ID" value="ENSPNAP00000016166.1"/>
        <property type="gene ID" value="ENSPNAG00000022379.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156813"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000004">
        <property type="entry name" value="vacuolar protein sorting-associated protein 33B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="70576" checksum="E7F08C347078D3E8" modified="2018-12-05" version="1">MAHTGRRDAPELPDFSLLKRLARDQLIYLLEQLPGKKDLFIDADLMSLLDRIANVTTLKQHEVDKLYKVELKPIVSTSDQLCFLIRPRIQTVKWIADIVNSDKASGRFRRYKIIFTPQKFYACETVLEEQGVYGDVTTDEWAFYLLPLDDDIISLELPEFFRDNFLEGDQRWVTTAGSALRLVYSLYGPFSKVYGIGRCAKMVYESWRDQVEDGEQKARQPEIGNVFLIDRDVDFVTPLCSQVVYEGLVDDIFRIKCGSVEFGPEVTSSDKSLKVMVNSQDKVFNEIRNEHFSNVFGFLSQKARNLQTAYDKRRGMDIQQMKTFVSEELKGLKQEHRLLSLHIGASECIMKKKTKQDFQELLKTEHSLLEGFEVRECISFIEEHINRQVRQHKQSKTIVVDLLRTQRCRSSLCSYNLINRNISYGIEHLLTFANLKQIGLLVEQQPGETLTVMESKVGKLVNDKTAGKLTDAFSSLAKKSNFRALSKRLVLIPKSGEEYDLRVPRDMAYIFSGAYIPLSCKLIEQVLERDGWTGLEEVTRMLNGHEFAVTGGTNGTEARIKTDPQRIILVMFLGGCTYSEISALRFLGREKGYKFIVLTTAITNSARLLEALLDNHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D209</accession>
    <name>A0A3B4D209_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D209"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025519.2">
        <property type="protein sequence ID" value="ENSPNAP00000016904.2"/>
        <property type="gene ID" value="ENSPNAG00000023221.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166432"/>
    <dbReference type="OMA" id="RINFNIM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF114">
        <property type="entry name" value="PCDH2G3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043467217" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="29"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="686"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="27"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="132"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="241"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="354"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="451"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="575"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016904.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="801" mass="87823" checksum="34D41C6B57A59801" modified="2024-11-27" version="2">MDCKVELKMRGPLFWSLFLLLELHPCNGDLSYSLSEETKRGHAVGDLAKDLGLDAKTLAARNARLDDSSKRYADLNLNTGDLIVAETMDREMLCGSRMNCILNYEIVLENPLEVHRVSLNIDDINDNAPKFLNERITFEIHESAAKGQRFRLDEARDSDIGQNGVNGYSLQRNDHFSLFVHDNKNGRKYAELVLDKELDREQQKDIDLILTAVDGGNPQRSGTATIHITVLDANDNVPVFSQPVYKVSLAENAPLGAEVVTVSATDADEGANGAVSYEFSNIADDAAQLFTINKVTGQMKVSGDIDYEKEAYYEIGVQAKDGSGLASTANVIIDISDINDNAPKIMLTSLKNPIPENAAVGTEVAIINVQDKDSAENRQIRCSIQQNVPFKLNPSIKNYFSLVTTSPLDREKEADYNITITATDGGSPPLSSFKTIHISISDVNDNPPVFEEPSYNAYVAENNKPGTSICSVTARDPDWRQNGTVLYSLVSSEVNGVPVSSFLSINADTGVIHAVRSFDYEQFRNFKVQVVARDNGSPPLSSNATVSVFITDENDNSPQILYPAPEGKSLMTEMVPKAALSGSLVSKVIAVDADSGQNAWLSYHIVKSTDPGLFSIGLHSGEIRAQRDISESDSMKQNLVISVKDNGQPALSTSCSVYLLISDNLAEVPELKDMTYEESSSKLTSYLIIALVSVSTFFLTFIILILAVRFCRRRKPRLLFDGAVAIPSAYLPPNYAEVEGAGTLRSSYNYDAYLTTGSHTSDFKFIRSYNDNTLPATLTLKGNHIYLVQKLLGFIAAVYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7Z1</accession>
    <name>A0A3B4D7Z1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NTF2-related export protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NXT2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017545417.1">
        <property type="nucleotide sequence ID" value="XM_017689928.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017545418.1">
        <property type="nucleotide sequence ID" value="XM_017689929.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017545422.1">
        <property type="nucleotide sequence ID" value="XM_017689933.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017545425.1">
        <property type="nucleotide sequence ID" value="XM_017689936.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037396445.1">
        <property type="nucleotide sequence ID" value="XM_037540548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7Z1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039360.2">
        <property type="protein sequence ID" value="ENSPNAP00000019236.1"/>
        <property type="gene ID" value="ENSPNAG00000025785.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108417324"/>
    <dbReference type="CTD" id="55916"/>
    <dbReference type="GeneTree" id="ENSGT00940000156381"/>
    <dbReference type="OMA" id="HFTRLYY"/>
    <dbReference type="OrthoDB" id="25408at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000006">
        <property type="entry name" value="NTF2-related export protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="5">
        <location>
            <begin position="17"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="143" mass="16479" checksum="4C8BAF69B5998BF6" modified="2018-12-05" version="1">MASTIDFRTHVDQLCRYSEEFVNIYYDCMDKKRRNLTRLYLDKATLVWNGNAVSGQDALGEFFESLPSSEFQVHTLDCQPVHEQATQGQTTLLVVTAGSVKFDGNKQRYFNQNFLLTAQPSPHSDQPVWKIASDCFRFQDWAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBM7</accession>
    <name>A0A3B4DBM7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552581.1">
        <property type="nucleotide sequence ID" value="XM_017697092.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBM7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020875"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031775.2">
        <property type="protein sequence ID" value="ENSPNAP00000020875.1"/>
        <property type="gene ID" value="ENSPNAG00000006215.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427142"/>
    <dbReference type="CTD" id="794920"/>
    <dbReference type="GeneTree" id="ENSGT00940000157535"/>
    <dbReference type="OMA" id="MFYESNG"/>
    <dbReference type="OrthoDB" id="8872282at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.900">
        <property type="entry name" value="transmembrane domain of human cd4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF11">
        <property type="entry name" value="NECTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017484188" description="Ig-like domain-containing protein" evidence="14">
        <location>
            <begin position="21"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="20"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="136"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="231"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="435"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="355"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="567" mass="63812" checksum="95899CAA700198E2" modified="2018-12-05" version="1">MARLAWFGLFLTKLIASVCGESVQPVGLRTFADEETRLPCEFKVQDESKVVQVTWSREQQDGVKEQIITNHMTEGIQEFPGFVGRVRFESLDPFKDSALLILSTKESDDATYTCHISTFPSGNFEKQIRLTVWRIPIAMLEPVILEEGQTFRVAATCRAVGLPRPELSWDTDLLGQTQNKSSEDKVISSTFLLHPLRSLNGRKLDCLVWHPALENPQRLSNTLVVHFPPNPEVVGYDQNWSVGRIGAGLRCESGGNPRPQNFTWNRKGKSLPEGVTVEGNRLFFTRPLNKTDEGVYECEAKNSMGAVRAEFNVDIPVTSEAARLEASFNNLLIIIVGGATGGLVVLMVIIIILVNRHHRRRNRKLKRELTERKEEINNLSRQASMRRLNSVSTDPRIQTEECALIRLDSALKSSFLSLEGNSTLCDQRDGLREGEYDSLGRPAIYPSYRSERGSSRRKETEVEREERRRRVESFVTNSTVPLDSDSLHRDQSPPPLSISSGPQREGGMEKEGWGQREGVTEGEEGDCTSNSYQISEALSNHFQYSNGFLRPKPHPNAILLHPRGQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDJ8</accession>
    <name>A0A3B4DDJ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017573964.1">
        <property type="nucleotide sequence ID" value="XM_017718475.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDJ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021560"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040631.2">
        <property type="protein sequence ID" value="ENSPNAP00000021560.1"/>
        <property type="gene ID" value="ENSPNAG00000028726.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439845"/>
    <dbReference type="CTD" id="447906"/>
    <dbReference type="GeneTree" id="ENSGT00390000003574"/>
    <dbReference type="OMA" id="NDMAETF"/>
    <dbReference type="OrthoDB" id="10265679at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008658">
        <property type="entry name" value="KAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05804">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="587"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="225"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="789" mass="90483" checksum="14261E48B75B8D65" modified="2018-12-05" version="1">MQADDVRYLTRKVKGGSLDVHPTEKALVVHYEVEARILGQTGDAMLGERKSCQKIIRLKSLSPNTDIGSLARKVVEECRLISPSKLPEVEQLLFYLQNRKSPTEKKEKKPIKPRDPTPFEGMELDEEANINSIDDYVELLYEDVPEKIRGATLILHLARNPDNLEELLQNETALGALARVLREDWKQSVDLATTIIYIFFCFSSFIQFHGLITYYKIGALCMNIIEHELKRYDLWQEELQKKKKAGEDDPENQNLKKEYEKALKKYQGLLTKQEQLLRVALYLLLNLSEDTRTELKMRNKNIVHLLVKTLDRDNEELLVLVVSFLKKLSIFLENKNDMADTDTVEKLAKLVPCEHEDLLNVTLRLLLNLSFDTGLRSKMVQVGLLPKLTTMLGDETQRHLAMRILYHISMDDRFKSMFAYTDCIPQMMKMLFDCVEERMDPELISFCINLAANKRNAQLICEGNGLKMLMKRALKLKDPLMMKVIRNVSQHDGPSKNLFIDYVGDLAAQIGQNEEEEFVIECLGTLANLTIPDLDWELVLKEYNLVPFLKDHLKPGCAEDDLVLEVVIMIGTVSMDDSCAAMLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATREVIIKETQAPAYLIDLMHDKNAEIRKVCDNTLDIIAEYDEEWGRKIQNEKFRWHNSQWLEMVENRQMDEAEPFMYSDEGEPFLQGGDILERPDLFYSADGIITADSAISPEFFTDFQNGDFIGQQGFPSSAVSDGFGQVAGGPLRPATAYGFRPDEQYYYSYSASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDK8</accession>
    <name>A0A3B4DDK8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coagulation factor IX</fullName>
            <ecNumber evidence="4">3.4.21.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Christmas factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.</text>
            <dbReference type="EC" id="3.4.21.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017581226.2">
        <property type="nucleotide sequence ID" value="XM_017725737.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDK8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021570"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032981.2">
        <property type="protein sequence ID" value="ENSPNAP00000021570.2"/>
        <property type="gene ID" value="ENSPNAG00000028661.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444525"/>
    <dbReference type="CTD" id="678552"/>
    <dbReference type="GeneTree" id="ENSGT00940000159516"/>
    <dbReference type="OMA" id="SYECWCR"/>
    <dbReference type="OrthoDB" id="8909918at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000013">
        <property type="entry name" value="Coagulation factor X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000162">
        <property type="entry name" value="Coagulation factor X (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF31">
        <property type="entry name" value="COAGULATION FACTOR IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="20 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 25" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="14 26" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="22" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 26" id="KW-0645">Protease</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="15 26" id="KW-0720">Serine protease</keyword>
    <keyword evidence="27" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043881959" description="Coagulation factor IX" evidence="27">
        <location>
            <begin position="23"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="30">
        <location>
            <begin position="42"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="28">
        <location>
            <begin position="88"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="29">
        <location>
            <begin position="256"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00031357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR001143-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021570.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="498" mass="55062" checksum="BF0D2361CE30C65D" modified="2024-11-27" version="2">MEKIKLFLLICGVLHETRLTSGASVFLSRQSAWSVLSRQKRYNTGAFEEMMKDNLERECIEESCTIEEAREVFENDEKTMEFWAGYIDGDQCNSSPCQNGGTCEDGMSAYVCWCPIGFNGKNCELEMARQCDVNNGGCMQFCLVDKTYGAVCDCADGYRLALDSRSCEPTAKYSCGRLSSHVAKAPSSRTLFSAGTVNRVHSVEHQNLTESNLTEPSSTVSTALSSATTRLATERDWAFFPTLPTITEKDNDDQRIVGGNEATPGEIPWQVALVATDKQLVFCGGSLLTEIWVVTAAHCLVDAQNTTYFVRLGEHDVRKEEGTESNHDIAEDHIHPSYNRQRGHSHDIALLKLKTPVTFSDYIIPICLGPKQFTENLLKSVPNSLVSGWGRLRFGGRESATLQKLEVPFVDRTECKGSDKISRFMFCAGYSSIQKDSCQGDSGGPHATNYGGTFFLTGIISWGQECAKEGKFGIYTRVSRYMNWITNITGIRAGSSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGV9</accession>
    <name>A0A3B4DGV9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="17">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">May contribute to UTP accumulation needed for blast transformation and proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 17">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with RNF19B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGV9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022144"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034088.2">
        <property type="protein sequence ID" value="ENSPNAP00000022144.1"/>
        <property type="gene ID" value="ENSPNAG00000029492.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230412"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043771">
        <property type="term" value="F:cytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000010">
        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000200">
        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="19">
        <location>
            <begin position="105"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="20">
        <location>
            <begin position="335"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="574" mass="64834" checksum="7C37DCAC8AE8B8C6" modified="2018-12-05" version="1">MRTGAERDENMEAAVKRAEVQKDPKEEREEDRPMSRSDSGSGEDSLDGLMRRNCPLTPSLSPRKRTTSQSKTEPPLLRTNKRTIYTAGRPPWYNVTGTTFKEAFVIGLCGGSASGKTTVANKIIEALDVPWVVLLSMDSFYKVLSNEEQELAAKNEYNFDHPDAFDFELLVNVLRKLKKGKSVKVPVYDFTSHCRRKEWKTVYGANVVIFEGILAFANKELLKLLDMKVFVDTDSDIRLVRRLKRDISERGRDIGGVIKQYNKFVKPAFEQYIEPTVQVADIVVPRGGENFVALDLIVQHVHSQLEKRKLRWDISALASAHQGQPLPKTLSVMESTPQVRGMHTIIRNKETNRDEFIFYSKRLMRLLIEHALSFLPLKPVSVETPQGTIYEGKRLSGKRITGVSILRAGETMEQALMAVCKDIRLGKILIQTNHDTGEPELHYLRLPKDISEDYVILMDSTVSTGAAALMAVRVLLDHDVQEDKIFLLSLLMAEMGVHSVAYAFPKVRIITTAVDKKVNDEFHIIPGIGNFGDRYFGTDAPSDWEPASSLTWLHLKVTAQPMLKGPSHPVHTWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHC5</accession>
    <name>A0A3B4DHC5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Akirin-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AKIRIN2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the akirin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHC5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011792.2">
        <property type="protein sequence ID" value="ENSPNAP00000023802.1"/>
        <property type="gene ID" value="ENSPNAG00000008291.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156096"/>
    <dbReference type="OMA" id="HQSECCY"/>
    <dbReference type="OrthoDB" id="10039914at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024132">
        <property type="entry name" value="Akirin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13293:SF8">
        <property type="entry name" value="AKIRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13293">
        <property type="entry name" value="AKIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="182" mass="20805" checksum="1128AD5F70262566" modified="2018-12-05" version="1">MACGATLKRTMDFDPLLSPSSSKRRRCAPVSSSSSSPRKYLRHEPSPFGEVSATLTTEQILHNIKQEYKRMQKRRHLDGSFPQSEACCSLESQSHSSILSGSSFPDTSSGAVSPTRREQPLFTLKQVGMICERLLKEREEKVREEYEEIMTSKLAEQYDTFVKFTHDQLMRRFEEQPASYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJ83</accession>
    <name>A0A3B4DJ83_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJ83"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010109.2">
        <property type="protein sequence ID" value="ENSPNAP00000024442.2"/>
        <property type="gene ID" value="ENSPNAG00000001274.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024442.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="79" mass="8526" checksum="89BCAC61E73C2432" modified="2024-11-27" version="2">FINIFIVEADLSISKAKVLKCPILPSTNNSHVCLFRFSGTSRKAGCILPTCAVHHLSEKLQAGDESAGDTHDPYGIGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMA0</accession>
    <name>A0A3B4DMA0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SYNPO2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the synaptopodin family.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000025527"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007386.2">
        <property type="protein sequence ID" value="ENSPNAP00000025527.1"/>
        <property type="gene ID" value="ENSPNAG00000001422.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183054"/>
    <dbReference type="OMA" id="DCNYNPH"/>
    <dbReference type="OrthoDB" id="6502734at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051976">
        <property type="entry name" value="Synaptopodin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217:SF9">
        <property type="entry name" value="SYNAPTOPODIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="245"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="487"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="752"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1290"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="391"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="776"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="820"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="839"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="891"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="911"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="925"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="956"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="966"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="979"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="999"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1019"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1052"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1138"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1148"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1160"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1195"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1242"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1292"/>
            <end position="1324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1481" mass="165059" checksum="0D0ED170E281EABE" modified="2018-12-05" version="1">MGAGEHAWITGWGFGLQHSGTQPNCTLRVDQVEEGSHASLAGLRVDDEIVSINREPSSSLSLSEAIALIKGSTDGLQLVVKRCCFQGGFQTVSTTLRIPAPYRPQGPKELCISDSQDEAFYGQTCSEAESPRQTPTVRTQVCIPAPKDRIGANTKSSGGGGTRLEIPPGSVVELQLSLSKTTLEDKGYTSLGSALGVEGDFCHKKNNEDDDTTTSPPGCTLYIPLHERQPLSQRGVLISGSSALHGQVEVTTQPLGRGPRRERVGSGETEAKASERGDYGEGEGGRTEKAPTEEAPTSSSVSFGIQSEEGDSESERELNKPNKHRPRHARLRRSESLSEKQVKEAKSKCKRIALLLTAAAPNPNNKGVLMFKKHRQRAKQYTLVSYGTGENEPEFEAEEDEEDNKETHAVEFTVLATSETELDEDFFTNPPGHKSIVTFDWDTGLLEIERKLHKPEDMNALPETKGKGALMFAQRRQRMDEIVAEHEDMRSKGIPVEAVQEEEKHYQQVEPRTYMPATESQNYMDVNVHQQQQYQQYQEQYYQQQQQQQYQEYQQQLQYQQQQQHYQQQQEYQQQQYQQQQEYQQRHYQEQQEYEQQQQYQQYQQQQQQMQQYSHQMNGMSSQQTNVMQSSIMNRSAKPFSVQNTEAATFSPTSQEQPCYSVGQGEQIASRDERISVPAIKTGILQDNRMRNKAKPMFTFKETPKVSPNPELLNLLNRGDKKAGFESGPEEDYLSLGAEACNFLQSPKVKHKIPPPVAPKPHINPASPPWSPQPEIASQQFPQHAENNIPATAEAPGPEADPVPEMESSAIPKQEPSPTPAAELQEAPAQPLSQEHQEALNTWGPSEQQMLPDQQQETWNENQSQQHINNAQLVQPSPQVQAKSELPVSSWAPSPTQAHQQPPVSAWGPTEVQSQTLAQTQSHMQQPTWVTQPQVQVQPPTSIQTQSQPEPPWVTQSPPQTQSQPQPQPQPKPQPQSQPQPQMNSWATAPTQSPPQPPWAQSQASVQPSVGTWPQDLNQTQAQPPWVQQQQESQPLPSWTTPQQHPQPPWAQPQSQPQHQPPWVTQTQQQALPQQPVNAWTQPQSPVQAQPPWAQQAQPPLQPSSQVQQSQGPWASVPTQSQPQPPWPQQPQEHPQQMMNSWAPEQNQVQPPWSQSQPPAQAPPPWVHQPQQEAPPPHSQSQTVVSTWGPRPQQGPVSTTAPTETQKLNQSAKPWSPPQTTQPPLQRMGSYTFGTKVPSPTPTSSTMSPSGMGSAFEMPALRGKGAELFAKRQSRMEKYVVDSDTVQANKALGRSSSLSPPVRLSPLGLSSRSTSVSPSPRPSSYATYTSCSTERQAIWSEKSRKPPTPWEAASRHPLGLVDEAFDFQDLQHCIATNLRSAAHRKLLPEPPADWKAKVAYEPPTKSQGWGTSRLSLPFLSPTKSTASAPAAPVPYGSPLRQSQPHRSITEANLGAFLVRPENKRILGQSSYKSTYSYTWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DP10</accession>
    <name>A0A3B4DP10_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017572154.1">
        <property type="nucleotide sequence ID" value="XM_017716665.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DP10"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008176.2">
        <property type="protein sequence ID" value="ENSPNAP00000025208.2"/>
        <property type="gene ID" value="ENSPNAG00000010225.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438683"/>
    <dbReference type="CTD" id="564994"/>
    <dbReference type="GeneTree" id="ENSGT00940000156082"/>
    <dbReference type="OMA" id="CKXMEST"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12470">
        <property type="entry name" value="RRM1_MSSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12244">
        <property type="entry name" value="RRM2_MSSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000450">
        <property type="entry name" value="RNA binding motif, single stranded interacting protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000014">
        <property type="entry name" value="RNA-binding motif, single-stranded-interacting protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034404">
        <property type="entry name" value="MSSP1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="56"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="135"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="372"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025208.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="397" mass="43099" checksum="75F8992FDFF13EEF" modified="2024-11-27" version="2">MIFANTANPVRPPYRKQPLVAPPTLPMAPPSPSTNSSTNNSSSNSSTTGWEQLSKTNLYIRGLPPSTTDHDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPVSAQKAVAALKSSGVQAQMAKQQEQDPTNLYLSNLPVSMDEQELENLLKPFGQVISTRILRDAHGVSRGVGFARMESTEKCDAVISHFNGKFIKSTSGMLGTSEPLLCKFADGGQKKRLSQTKYVPNGRTWTRDAEVRLSGITLTYDPNATALQNGYYAPPYSMGNRIMAQTAVTPYISPVSTYPVQNPSWMTHQPYIMQHPGAVLSPSMEHPMPLQPSSMLAPLTQQMGHLSLGGTATFIPANTAMQGAYIPQYAHIQPAAIATEDPSVQPQDVTSSDPSTYPFQPSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQ35</accession>
    <name>A0A3B4DQ35_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alcohol dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="9">1.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">S-nitroso-CoA reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids. Acts as an aldehyde-detoxification enzyme. Also acts as an inhibitor of protein S-nitrosylation by mediating degradation of S-nitroso-coenzyme A (S-nitroso-CoA), a cofactor required to S-nitrosylate proteins. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADPH-dependent reduction of S-nitrosoglutathione. Displays no reductase activity towards retinoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>S-nitroso-CoA + NADPH + H(+) = sulfinamide-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:78375"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145546"/>
            <dbReference type="ChEBI" id="CHEBI:145548"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:78376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-nitrosoglutathione + NADPH + H(+) = S-(hydroxysulfenamide)glutathione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63500"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.2" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017542789.1">
        <property type="nucleotide sequence ID" value="XM_017687300.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQ35"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025565"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007286.2">
        <property type="protein sequence ID" value="ENSPNAP00000025565.2"/>
        <property type="gene ID" value="ENSPNAG00000010740.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414433"/>
    <dbReference type="CTD" id="445326"/>
    <dbReference type="GeneTree" id="ENSGT00940000166020"/>
    <dbReference type="OMA" id="FMTMKAA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046185">
        <property type="term" value="P:aldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19106">
        <property type="entry name" value="AKR_AKR1A1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000006">
        <property type="entry name" value="Aldo-keto reductase family 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044481">
        <property type="entry name" value="AKR1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="18">
        <location>
            <begin position="18"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="17">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025565.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="325" mass="36743" checksum="DE5547D92F349F23" modified="2024-11-27" version="2">MASTTMTLSNGLQMPVVGLGTWKSAPGQVKQAVLAALDSGYRHIDCAAAYSNEHEVGQAIAERIGPGKLLRREDVFVTSKLWNTKHHPDDVEAACKRTLSDLRLNYLDLYLMHWPMAFERGAELMPRRADGTIRYADTHYRDTWAAMEKLVDQGLVRAIGLSNFNARQIDDILSFGKHKPVVNQVECHPYLTQSQLLSHCQSQGLVLTAYSPLGSPDRPWASPSEPLLLDDPRLVAMANRYKKTPAQIIIRWLVQRGAACIPKSVTPSRIKQNIEVFDFKLSDEDMKEIESFNRNERVIIPTIEKDGQKVWRDASHPHFPFHDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DRE0</accession>
    <name>A0A3B4DRE0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger protein 644b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DRE0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026038"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006086.2">
        <property type="protein sequence ID" value="ENSPNAP00000026038.2"/>
        <property type="gene ID" value="ENSPNAG00000011330.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158258"/>
    <dbReference type="OMA" id="SSKQANC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060219">
        <property type="term" value="P:camera-type eye photoreceptor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070050">
        <property type="term" value="P:neuron cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000409">
        <property type="entry name" value="zinc finger protein 644 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051643">
        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF25">
        <property type="entry name" value="ZINC FINGER PROTEIN 644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="178"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="316"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="539"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="667"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="712"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="550"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="672"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026038.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="759" mass="85960" checksum="35CE506D1CA3FB62" modified="2024-11-27" version="2">MIYHLDRHNQVGQESVLRPFICKECGRSFRERSTLHKHMQIHQARRERLMQEIKGLNESRDDGPDGQLQCPQCAFETSCPKTFVHHTKTHEQDKRYYSCEEHDYPSVSELDVEAHQRTAHEGRFLKVFDTFFCKICTFRTKNVSVLRKHLELVHKQPLYDYDLQSHNLTADNHIKPVLDPDRLTDQTKDDISPPLSLKPKSLRDKQNGWRGAGLSMRSNGTADLYVRDKGTQKSCKGLSSSLIKWSFGSLANNLSPSSLRCDKPSKLSVPTERMDVTTGLPYVEEDNQGYESPVSEQKTKHLSSFDMHLTTKTAISSKTAHLNQDTATASKDPSESGGLVEPAVKQKSPSKRKMSIPYHNAPKNTLIFPKCEPVSPEWQDTDPLEESDYDDANDANESNDANYHDTTANLLDGSENGHSPYFADRFFRQGFSLKEDSCDSANHSEHDDYMEGDEICTLIVKEESIESAVSEDNPELEFPDHNLSDSCTSYRVSPVFEMDRKSCPYCPAVFESGVGLSNHVRGHLHRLGLSYDARHMLSPEQVASQDRQPRIRRRAPSMNRRTRKDKPESQTEHTCPLCLGWFDTKTGLSNHVRGHLKRIGKPITGVSKSPLCILTELLQDENEHQKILHSLQAKPHLSRPFISQKFAGSEGLFLTPTGVPVKVQHQWPSSELRSEDGEKKKKSEEISVMNETSSSTLIELLKRKRLDPELEEVKGQAARTRYVVPSSRSADSEDLPPQDLSSSCSRGEERTVSHLGWVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DT97</accession>
    <name>A0A3B4DT97_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NXPE C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NXPE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DT97"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002154.2">
        <property type="protein sequence ID" value="ENSPNAP00000027592.2"/>
        <property type="gene ID" value="ENSPNAG00000013114.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182866"/>
    <dbReference type="OMA" id="MWHSLSC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057106">
        <property type="entry name" value="NXPE4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165:SF23">
        <property type="entry name" value="NEUREXOPHILIN AND PC-ESTERASE DOMAIN FAMILY, MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165">
        <property type="entry name" value="NXPE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NXPE C-terminal" evidence="2">
        <location>
            <begin position="278"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027592.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="500" mass="56319" checksum="831461C47F59D24C" modified="2024-11-27" version="2">KKIFCVTFIVDLVLQKRLSVCSNNSTVSQSDSAHSSTNDPTFISPEELQTIEKALKWPAPPTSMIAANQTTSPYTTSYDLQNPRTSYSVGDIITVVITARDGLKNSKTYGGDFFQAKLFNTKLKASVYGEVTDHHNGTYTATFKLLWEGQAKVSIRLIHSSEAVQVLLRHRERDPDKVFFVGVFRGVDHQEKVVCNAQRSERLLKASQCCCEYKHPISGELWFCHRPSYLPCSAWVDHGMGGYQAQLSKLDSTLLHSVKCVPGLDTPSPAGFYLHDTWTSLMCHAQSFPTANQIASCLKDKQVLMMGDSTLRQWFEYLLKTVPTLKKLNLYTSAQSGPLEAVDTCNNIRLVWRAHGLPIRTFMVPRADLHYIASEVDALAGGQHSVVVFTIWAHFTTFPLYTYLKRLAGIRRAIQALLQRAPQTLVLIKSANTGYKDIYGSDWLSWQLDCALRVMFRELPVVIIDVWQMTSCHYSPDNIHPSHEVIKNEVDLFLSFVCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTF4</accession>
    <name>A0A3B4DTF4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CPEB3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPEB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552169.1">
        <property type="nucleotide sequence ID" value="XM_017696680.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTF4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026748"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004360.2">
        <property type="protein sequence ID" value="ENSPNAP00000026748.1"/>
        <property type="gene ID" value="ENSPNAG00000012111.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426867"/>
    <dbReference type="CTD" id="22849"/>
    <dbReference type="GeneTree" id="ENSGT00940000158949"/>
    <dbReference type="OrthoDB" id="10033548at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008135">
        <property type="term" value="F:translation factor activity, RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000766">
        <property type="term" value="P:negative regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12724">
        <property type="entry name" value="RRM1_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12726">
        <property type="entry name" value="RRM2_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000008">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.40:FF:000001">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000009">
        <property type="entry name" value="cytoplasmic polyadenylation element-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032296">
        <property type="entry name" value="CEBP_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034819">
        <property type="entry name" value="CPEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT BINDING PROTEIN CPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566:SF7">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16366">
        <property type="entry name" value="CEBP_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16367">
        <property type="entry name" value="RRM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="434"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="542"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="691" mass="76482" checksum="E523F7AB0E0B7738" modified="2018-12-05" version="1">MKIHIWLQNTSYCARQTMQDDLLMDKSKAQAHQQQQDPASIDPPSTPTPSSEPAVSEDSPSGQAPSRPSGKEKTPMESPLLPGLSFHQPPPPPQETGGTSLSPSFGSTWSSPTTNAMDDSFFQGIPSVNGTMLFQNFPHHVNPVFGGSFSPQIGLAQSQHQQQPQPPPPQQQPQSQSQQRRSPVSPNQAAFPQRTAYAHQAVVNSKVSPASSSSSSSAWNNHQNAVWSSGSSPWSGLQAARDPRRAVGVGVGVGVGVGVGVPSPLNPISPMKKPYPSNVIAPPKFPRAGPLNPKPWMEDNAFRTDNSNNMLPFQDRNRPFDPFSLQTLENSLMDMIRTDHDKGKQHPVGGPPMTIADIVWRNHFAGRMGLNFHHPAAEHIMPLTRNYGRRRGRSSLFPFEDGFLDDPHGDQSLTPGLNSPTRCQNGERMERYSRKVFVGGLPPDIDEDEITNSFRRYGHLVVDWPHKAESKSYFPPKGYAFLLFQEESSVQALIDACIEEDGKLYLCVSSPTIKDKPVQIRPWNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHNDIDKRVEVKPYVLDDQMCDECQGARCGGKFAPFFCANVTCLQYYCEYCWASIHSRAGREFHKPLVKEGGDRPRHVSFRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZ94</accession>
    <name>A0A3B4DZ94_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MRM1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553097.2">
        <property type="nucleotide sequence ID" value="XM_017697608.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZ94"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028745"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035136.2">
        <property type="protein sequence ID" value="ENSPNAP00000028745.2"/>
        <property type="gene ID" value="ENSPNAG00000014551.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427448"/>
    <dbReference type="GeneTree" id="ENSGT00390000018761"/>
    <dbReference type="OMA" id="QVPPYEY"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000054">
        <property type="entry name" value="rRNA methylase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="11">
        <location>
            <begin position="135"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028745.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="390" mass="42553" checksum="2A658E240C5724E4" modified="2024-11-27" version="2">MWRAACRCSCVIRNNRYSPVELLGVLYCGETQYASYHSTKPLLLPKDTGLKLVERLRCNLFTSSGKLSRTAGFPSTKRPQSKEPPASYPGRKVSSELQKLSLEDFTEAEAQKLNKGTQKVRTAQVKVRGAKEHDMVFGVAPCLLALTQARRTPSRLFVKSSEGHPREAVQRVCEEALRHGVPIQRVSKRELDTMTGGMVHQGVCLQASPLGFLTKEKSTRPQHGGHPHPLWLVLDGVQDPMNLGSILRTAYFLGVDRVASSIRNSCPLTPVVSKASSGVMELMDVYGYSNLADVVKMKLEQGWQVVGTVGSEEAVSGASVVSCSEYRMSKPTLLLMGGEGFGLSPELRQLCDILLTVPPRRDLHPAVDSLNVSVATGILLHSILSSCRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0J5</accession>
    <name>A0A3B4E0J5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">SEC24 homolog A, COPII coat complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SEC24A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0J5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028826"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015357.2">
        <property type="protein sequence ID" value="ENSPNAP00000028826.2"/>
        <property type="gene ID" value="ENSPNAG00000014660.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182924"/>
    <dbReference type="OMA" id="AVECSKQ"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000004">
        <property type="entry name" value="SEC24 homolog B, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000019">
        <property type="entry name" value="SEC24 homolog B, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF1">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC24A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="16">
        <location>
            <begin position="152"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="17">
        <location>
            <begin position="225"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="19">
        <location>
            <begin position="469"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="18">
        <location>
            <begin position="564"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="15">
        <location>
            <begin position="691"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028826.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="91440" checksum="A2FE45B7B850C61C" modified="2024-11-27" version="2">MSKPAGTAYPSLEPGYQGIEGIPKPLPVHPPTPLYGFPPQQYQQPSVGPAQLSPSLSALSLQSQTPEALRVVNLLQERNILPPGPVPAPTPCLPQDLQKLNCSPEVFCSTLTSIPQTQALLNKAKLPLGLLLHPFKDLSVSPQLPVVMSSSIVRCRSCRTYINPFVSFLDQRRWKCNLCYRVNDVPEDFMYNPVSRSYGEPHKRPEIQNATIEFIAPSEYMLRPPQPAVYLFVLDVSHNAVETGYLDVVCQSLLESLNSLPGDTRTKIGFITFDSMIHFYNLQEGLSQPQMLIVSDIEEIFLPTPDSLLVNLNECKELVRDLLKSLPQMFTKTMETHSALGPALQASFKLMSPTGGRVSVFQTQLPSLGVGALNSREDPNQRASAKDVQHLSPATDFYKKLALDCSGQQVAVDLFLLSGQYCDLATLGCVSRYSAGSIYFYPSYHSQHNPAQTERFQKDLKRYLTRKIGFEAVMRIRCTKGLSIHTFHGNFFVRSTDLLSLPNINPDAGFAVQMSIEENLVDMQVVSFQAALLYTSSKGERRIRVHTMCLPVVNSMSDIFAGADVQAITGLLASMAVDRSVTASLSDARDAMTNAAIDSLISYRTSVLTIQQPGLLAPACLRLFPLYILALLKQKAFRTGTSTRLDDRVFAMCQLKYQPLMYLMLMIHPSLYRLDNLTDEGSLNINDCTIPQPGMLQLSVEKLNSEGAFLMDTGSVSMYLWVGRNCNPTFLKQVLGVQNYATLPLNMNRLPELDTAESTRTRAFISWLREQRPFYPLLHIVRDESPLKASFMQNMIEDRTESALSYYEFLLHVQQQVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E130</accession>
    <name>A0A3B4E130_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017547063.1">
        <property type="nucleotide sequence ID" value="XM_017691574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E130"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029026"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035303.2">
        <property type="protein sequence ID" value="ENSPNAP00000029026.1"/>
        <property type="gene ID" value="ENSPNAG00000014886.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423916"/>
    <dbReference type="CTD" id="562065"/>
    <dbReference type="GeneTree" id="ENSGT00940000158179"/>
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    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046356">
        <property type="entry name" value="MARCHF4/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="116"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="392" mass="43472" checksum="80B3B490B4943C28" modified="2018-12-05" version="1">MLSGPGMLKGRCCVLFSDLKVLLLRPQASQVPQAQQAPQAPQSPPVLSPMSGPPATRCESEGNPCSCAANNNNTSFRLQHWSIPSTEGAVGPDAQRATAAEEFSKDKRDERFSFISYAEGTPVCRICFQGPEQGELLSPCRCSGSVRSTHQPCLIKWISERGSWTCELCYYKYQVIAISTKNPLQWQAISLTVIEKVQIAAAILGSLFLIASISWLVWSSFSPSAKWQRQDLLFQICYGMYGFMDVVCIALIVHEGPSVFRIFHRWQAVNQKWKVLNYDKKRDSEELKASESEWTLSLLQQQEQAGAAISPSTSSLMAAAAMASPETPNAMALHTDSTHNHDNGSAMTDHHCPYNFLHLLSHLRPLELRSQPSNTSNSSHQPRELVMRVTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E147</accession>
    <name>A0A3B4E147_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lysosomal amino acid transporter 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">PQ-loop repeat-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Solute carrier family 66 member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SLC66A1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Amino acid transporter that specifically mediates the pH-dependent export of the cationic amino acids arginine, histidine and lysine from lysosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the laat-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571941.1">
        <property type="nucleotide sequence ID" value="XM_017716452.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E147"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029433"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016535.2">
        <property type="protein sequence ID" value="ENSPNAP00000029433.1"/>
        <property type="gene ID" value="ENSPNAG00000015540.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438560"/>
    <dbReference type="CTD" id="54896"/>
    <dbReference type="GeneTree" id="ENSGT00390000003344"/>
    <dbReference type="OMA" id="DMCIFIQ"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080144">
        <property type="term" value="P:intracellular amino acid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000013">
        <property type="entry name" value="lysosomal amino acid transporter 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000017">
        <property type="entry name" value="lysosomal amino acid transporter 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF36">
        <property type="entry name" value="LYSOSOMAL AMINO ACID TRANSPORTER 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="315" mass="34766" checksum="9E3A40C38F9D980E" modified="2018-12-05" version="1">MSDGAFSSGGGFLSDGDGNFSTLCPNGTAWVWYGLQECAQDARDMASVILGLLSIVCFMVSSFPQYYKSCRTGNMDSALSIWFLLLWLAGDSCNLIGSFLADQLPLQTYTAVYYVFADLIMLSMYSYYKLRNKMTNRELIGGDRAVLNAVGVLCLLGVSASLIRLPVQYEQSVAVSGFRVRSLLAVNEQIGSIQAFTTKEIIGFTIGSASSMLYLCSRLPQMYTNFRRKSTEGVSYFLFALVILGNITYGLSVLLKNPDHGQGEGSYIVHHLPWLIGSLGTLTLDLLISVQFMIYRRAPLDNYNRAEESAPLLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3P7</accession>
    <name>A0A3B4E3P7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoinositide interacting regulator of transient receptor potential channels</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3P7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031242"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036628.2">
        <property type="protein sequence ID" value="ENSPNAP00000031242.2"/>
        <property type="gene ID" value="ENSPNAG00000018474.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176898"/>
    <dbReference type="OMA" id="FPIHFMS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028068">
        <property type="entry name" value="PIRT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100:SF4">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15099">
        <property type="entry name" value="PIRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031242.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15561" checksum="F73B3A2B2568277D" modified="2024-11-27" version="2">MALPPPPEHIPLGERSRSLSPEQLTPYTESTIFSLSRSESLWHSSTARTSCEFYRHAILLMTTGGSVLACGIVLSGLFFSGFSIKATKILGPALLSIGLVVLVVGVVLVPITKEMKQQSSLKRMSTFTRNYPHFMIFLYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4G3</accession>
    <name>A0A3B4E4G3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4G3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030583"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018775.2">
        <property type="protein sequence ID" value="ENSPNAP00000030583.2"/>
        <property type="gene ID" value="ENSPNAG00000017411.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286997"/>
    <dbReference type="OMA" id="TERIQAN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF511">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00592">
        <property type="entry name" value="CASENSINGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="13">
        <location>
            <begin position="12"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="GPCR family 3 nine cysteines" evidence="14">
        <location>
            <begin position="445"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030583.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="64601" checksum="240961593DE14E6F" modified="2024-11-27" version="2">MFCSFGARALRWMRTMTFAVKEINQRQDLLPNLSLGYHIRDSFDEIPLSIKGSLVLVNGQSLHLYIDIYEIKCLINVNFISTAWLTCFFMTSSQVSYFSSCSCLSNKKDFPTFMRTMPSDQFQIKALARLVHYFQWTWVGVIGVESDYARFAIQLFLKESASYNVCPAYVHIYPIVLTEEALRELMNTIRASTATVIVSFSAETDMRTVLKECRRQNITYVQWIASEAWATSKSLWGDYSNILQGTLGFAIRKGEIPNLGNYLRGLNTSTVQASDFLTEFWEETFNCRVNGSLNTHIHKEDAQKRGPCSGRESLDNVYTSYADVTQLRVSYNVYKAVYLIAHALHEMNTCVPGQDKKTNNVQIWPISCLSYQSSSKLLSYMKRTNFTTLGEDVRFDENGDPIASYDLMNWHTTKDGSLQLIRVGFYDASLREDRDLVINESVIAPVSVCSKSCLPGSRKARRKGEPICCFDCIPCAEGEISNETDSIDCLRCSKEMWPNQARDQCIPKALEYLSFQEPLGIVLWSVSTFGACLTAAVLCIFVIVCCAGMRGSETAVLLEFLNTVSTHCPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E532</accession>
    <name>A0A3B4E532_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E532"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030339"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018289.2">
        <property type="protein sequence ID" value="ENSPNAP00000030339.1"/>
        <property type="gene ID" value="ENSPNAG00000016999.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163982"/>
    <dbReference type="OMA" id="HSCCLLI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050657">
        <property type="entry name" value="Ankyrin_repeat_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24147:SF53">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24147">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="303" mass="33077" checksum="4ECF9264A00994ED" modified="2018-12-05" version="1">MKKIFNFTKKKKGSPSASDTGSVLSVGYDLKEKDLGKVQKAAFTGDVAKLRQLAKKNDLNQLDKENRTALHIACVHGHAEVVQFLVESKVKLNLCDNQNRSALMKAVQCQQDRCVSILLEHEADPNMVDINGNTALHLAARIPSLSVALQLLEHKANINAQNKEGNTPLILSVEENHAEMAELLLKEGADVNIKNQEQRPPLMIAACNGHINMVRLLLQYNVDITVKDIKGWSSDDYAVMNGHHACSHLIIEHGTKKKSLQSPSHLAPGKKKQASMLGSPEAGFSLGGPALDKDGECLSFLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E533</accession>
    <name>A0A3B4E533_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor SOX</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017549941.1">
        <property type="nucleotide sequence ID" value="XM_017694452.2"/>
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    <dbReference type="STRING" id="42514.ENSPNAP00000030426"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018475.2">
        <property type="protein sequence ID" value="ENSPNAP00000030426.2"/>
        <property type="gene ID" value="ENSPNAG00000003468.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425639"/>
    <dbReference type="CTD" id="30602"/>
    <dbReference type="GeneTree" id="ENSGT00940000161652"/>
    <dbReference type="OMA" id="CEGASMY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:brain development"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:camera-type eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="HMG-box_SoxC_SOX11"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcription factor SOX"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="High mobility group box domain"/>
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        <property type="entry name" value="HMG_box_dom"/>
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        <property type="entry name" value="HMG_box_dom_sf"/>
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        <property type="entry name" value="SOX-12/11/4"/>
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    </dbReference>
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        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF113">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
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        <property type="entry name" value="SOX-12/11/4a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
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    </dbReference>
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        <property type="entry name" value="HMG_BOX_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="13">
        <location>
            <begin position="50"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="11">
        <location>
            <begin position="50"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="117"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="140"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030426.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="386" mass="42014" checksum="8499D955BA9BCF02" modified="2024-11-27" version="2">MVQQAQREECEGAGRGDAGADSDESEFAARSPAPGAEANPDWCKTATGHIKRPMNAFMVWSKIERRKIMEQSPDMHNAEISKRLGKRWKMLKDSEKIPFIREAERLRLKHMADYPDYKYRPKKKPKLDGANAGASKPEKAAPSPEKACAKASKSAASKRCPKAKASSKSGAKAAAHHHLHQQQFQHHHSDEYALKSARGAAGKSARHSMDFSDDDEDEHDEHSLEDECEERARVPASPTLSSPGECEGASMYEDVRGARLYYSFKNITKQQSGVHAYSLAHSASASPASSSSRSASTSSSGSSDTDDLLFDFSLNFAAAAAAHSAESGGQNAGGLALSLVDKDLDSFSEGSLGSHFEFPDYCTPELSEMIAGDWLEKNFSDLVFTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E6E1</accession>
    <name>A0A3B4E6E1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E6E1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030881"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019327.2">
        <property type="protein sequence ID" value="ENSPNAP00000030881.1"/>
        <property type="gene ID" value="ENSPNAG00000017868.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164962"/>
    <dbReference type="OMA" id="ACVFQDQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd18273">
        <property type="entry name" value="BTB_POZ_KBTBD6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047933">
        <property type="entry name" value="KBTBD6_7_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF23">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="36"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="593"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="627" mass="70904" checksum="E6745B0A9A51AC7E" modified="2018-12-05" version="1">MATVDCFSGPEELEDSSHAASLMKELKFFYDSRLLVDVTIEVEPEQDSSANSVTSDCASRAETGKLFQCNRNVLAAASPYFKSMFTGGLYESTQSKITIHDVDADSMSVIIDYCYTGKVTVTEGNVQRLYAAANMLQLEYIRHACADFMTRRLDLSNCTGVLKFADTFDNLDLKSKAQAFIAKNFALLSASGKELCELDMKQMKEILTLDSLDVDCERKVCSVAIHWIENNLLPESEDALHILKCVRWSLFTEKDRVYLDGLKSKPFIKKHLSGFIDLTGGGESSVISKGMNLTKQRIGRSAKEMVLFFGRPNEPFMCYDPYTEEIYSMGSPVINLSNQNFKRSPMETFLVCATPENNLYLASHLSKHFWVYNPLLNCWQELAERLLGRMHSYIGYLNGHLYILGGRDPVSDARLKEVECYSIQRNQWMFVAPLPHSLGKMQVVTVNERLYVVNKRRMLCYESKRNHWLQRGSLKRNKLHKACVFQEQIICLCDIPVVKAYNPARGEWRRIGDIPIDSSALSYQVVQHNGKLLLLTLAIVHHNKNRLVIHEYDSTRDTWKNVVTMFGSSFGSISLSARVYTACLSSGQNFITEEDDDSGSSADWDFDGLTDADSDSGSSSSFSDENW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7Z0</accession>
    <name>A0A3B4E7Z0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Functions in replication-dependent translation of histone mRNAs which differ from other eukaryotic mRNAs in that they do not end with a poly-A tail but a stem-loop. May participate in circularizing those mRNAs specifically enhancing their translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with EIF4G1, EIF4G2 and SLBP; probably tethered by SLBP to the 3'-end of mRNAs ending with the histone stem-loop, it also interacts with EIF4G1 which is bound to their 5'-end.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIF4GD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563583.1">
        <property type="nucleotide sequence ID" value="XM_017708094.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7Z0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020356.2">
        <property type="protein sequence ID" value="ENSPNAP00000031411.2"/>
        <property type="gene ID" value="ENSPNAG00000018722.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433490"/>
    <dbReference type="CTD" id="503596"/>
    <dbReference type="GeneTree" id="ENSGT00940000153432"/>
    <dbReference type="OrthoDB" id="6357832at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062073">
        <property type="term" value="C:histone mRNA stem-loop binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000025">
        <property type="entry name" value="MIF4G domain containing a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051367">
        <property type="entry name" value="mRNA_TranslReg/HistoneTransl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254">
        <property type="entry name" value="EIF4G DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254:SF17">
        <property type="entry name" value="MIF4G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MIF4G" evidence="9">
        <location>
            <begin position="22"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031411.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="246" mass="28451" checksum="EA0CBC5F82FB6896" modified="2024-11-27" version="2">MLLGVGWEQICTGILVSKHCVLKRMEDSSKEEYRIQSFDMETQKLLKTALKDPGSVDLEKVSNVIVDQSLKDQVFSKEAGRICFTIVQAEAKQSSSSVFRRNLLNRLQQEFKAREDTRKKSTQEWVCLVSFICNIFDYLKVNNMPMMALVHPVYDCLFRLAQPDALKNEEEVDCLVLQLHRIGEQLEKMNLQRMDELFCLLRDGFLLQDGLSSLGRLLLLEILEFRAGSWTLSETAQKYYYSEVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8Q5</accession>
    <name>A0A3B4E8Q5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KLHL29</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8Q5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031656"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020836.2">
        <property type="protein sequence ID" value="ENSPNAP00000031656.2"/>
        <property type="gene ID" value="ENSPNAG00000019137.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158824"/>
    <dbReference type="OMA" id="NPATCER"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="CDD" id="cd18468">
        <property type="entry name" value="BACK_KLHL29_KBTBD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF30">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="335"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031656.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="878" mass="95840" checksum="7435F97F34703EAF" modified="2024-11-27" version="2">MSRHNHRFERDFRPVWDRRERCGISSSCVVANGCGSTAVSATSVPSSGNNRPGLLPLPVIPSLLPTPVTGATTTSSTELACKLLSGSGVKYAAPGVWNMGENQTAGPALAPSIRWGQTPINQSTPWDTDEPPTKQMREKDNTGPLWPPAVATVSQASLLSHSYGMPQPPAYSQGVTVQAPIIGATPALQTPVPPPHPLLPTHYQLHQPPSQPLASMVLNVQSQPLGVSSLPPIAMSPMPGVCQVTHPVHTVLGNGHLAHPMVQPSTTLASNNLPLTNGQAATQHPPATAPITQETANGLQMLRTVGMGKYEFTDPYHPKEMLKELNQQRRAKEFTDLNIIVEGKEFEVHQNVLASCSLYFKDMVKRFASLLNGEKMQMTMNNISAEVLELLLEFVYTGSLIIDSANAKTLLEAANKFQFNTFCKVCVSFLEKQLTAGNCLGVLAMAEAMQCTELHNMAKAFALQNFPDVASQEEILNISKEDFISYLSNDSLNTKAEELVYETVIKWIKKDPCKRVQHVSELLAVVRLPFIHPSYLLNVVDNEELIKSSEPCRDLVNEAKRYHMLPHARQEMQTPRTRPRLSAGVAEVIVLVGGRQVIGMNQRSLTAVTCFNPQNNKWYPLASLPFYDREFFSVISAGDNIYLSGGMESGVMLADVWCYMSLLDNWNLVSRMTVPRCRHNSVVYDGKLYTIGGLGVAGNLDNVERYDTITNQWETVSPLPKPVHSAAATVCGGKIYVFGGVNEAGRSAGVLQSYVPQTNAWSFIESPMIDNKYAPAVTLNGFIFILGGAYARATTIYDPEKGNIKAGANMNHSRQFCSAVVLDGKIYATGGIVSSEGPALGNMEAFDPCANAWTLQQSMPCPLFRHGCVVIKKYIQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E984</accession>
    <name>A0A3B4E984_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FOXO4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017562550.1">
        <property type="nucleotide sequence ID" value="XM_017707061.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E984"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032370"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022264.2">
        <property type="protein sequence ID" value="ENSPNAP00000032370.1"/>
        <property type="gene ID" value="ENSPNAG00000020261.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432921"/>
    <dbReference type="CTD" id="4303"/>
    <dbReference type="GeneTree" id="ENSGT00940000159334"/>
    <dbReference type="OMA" id="VHTEGHS"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032364">
        <property type="term" value="P:intracellular oxygen homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001945">
        <property type="term" value="P:lymph vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20062">
        <property type="entry name" value="FH_FOXO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000032">
        <property type="entry name" value="Forkhead box protein O4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047409">
        <property type="entry name" value="FH_FOXO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032067">
        <property type="entry name" value="FOXO-TAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45767:SF3">
        <property type="entry name" value="FORKHEAD BOX PROTEIN O4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FORK_HEAD_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="9">
        <location>
            <begin position="94"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="7">
        <location>
            <begin position="94"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="332"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="631" mass="66176" checksum="BC84FF8B6F2AD2D8" modified="2018-12-05" version="1">MEAESVPPIDPDFEPQSRPRSCTWPLPRPDISAVKAEGTDGAESTAGTPPAEEDKHEPQQIRAEPEKAVASEGGVVAGVGGATPRKGSSRRNAWGNQSYADLISQAIENSPEKRLTLAQIYDWMVKTVPYFKDKGDSNSSAGWKNSIRHNLSLHNKFLRVHNESTGKSSWWMLNPEGGKTGKAPRRRATSMDNSTKLLKSRMRAKQTKKQAGAGGIGGPGGAIQGEGVSGAGGADSPGPPGQFGKWGVNSSSPSSRGSLDDQDMWTGFRPRTSSNASTLSGRLSPIAPGQEDEDNLPEDGLLSGYTSGSLPPTLTETLMEELDLIDGLTLMTSQQGGASPSTAPQAPPTPLPSASTLLPRGTNFPSYRQLQPVKGPQGPTQPGSQSSVQQGGPSSTSPSNFGNSLFNPLPGPGSRSGFSTHVPSSLEALLTSDSPPPSDVMMTQVDPLMPSQGGTNLLGLAGSMSGNQAKPSQIMLGKSMEPSPVGQMTLQPQTQLQPQQQQHAQLNFGMLLSGMGQEPAQLTAMKTQHQVPGGGLLQHGAVPSAGGNGGVQGISQFGTPACFLPSQDRLPTDLDIDMFTENLDCDVDYIINSDLMDGDAIDFNFDPIVPAGQSYPGPATTQSSSHNWVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAM2</accession>
    <name>A0A3B4EAM2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548808.1">
        <property type="nucleotide sequence ID" value="XM_017693319.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EAM2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033597"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024665.2">
        <property type="protein sequence ID" value="ENSPNAP00000033597.1"/>
        <property type="gene ID" value="ENSPNAG00000022340.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424969"/>
    <dbReference type="CTD" id="25873"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="60S ribosomal protein L36"/>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_L36e"/>
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    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_L36E"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="105" mass="12279" checksum="4B988C8BBF26CEBB" modified="2018-12-05" version="1">MAVRYPMAVGLRKGHPVTKNVSKPRHSRRRGRLTKHNKFVRDMIREVCGFAPYERRAMELLKVSKDKRALKFIKKRVGTHIRAKRKREELSTILAAMRKAAAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EBC5</accession>
    <name>A0A3B4EBC5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EBC5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032998"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023461.2">
        <property type="protein sequence ID" value="ENSPNAP00000032998.1"/>
        <property type="gene ID" value="ENSPNAG00000021326.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182966"/>
    <dbReference type="OMA" id="VPVWIYT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004974">
        <property type="term" value="F:leukotriene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000109">
        <property type="entry name" value="Leukotriene B4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF946">
        <property type="entry name" value="LEUKOTRIENE B4 RECEPTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="35"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="305" mass="34209" checksum="A9EDA0067BDF2864" modified="2018-12-05" version="1">MQLSNSSDLSSTGLSQANLVSSVLMGFFCAVGVPGNIAVIVIIVRWFKKDNFTVHLMLNLAASDILCLMTLPVWMYNQLHGWTIGRSLCKLISSLAYSSVYSSLLTVTLMSVHRCLQICFPEIWAKLGKTGERFLLFMIWACGFAFSVPAILTQEIVQRESKRECGRITSSDEYRLVVAVLETLLAFVLPCSIMVISYYCLHKRVTQKALIRSRRLTKLVTRIVVTFFVFWTPYHILNLVELFAICLKQSYPGSSGKVLVFTASLGDIAGSFTFINSCVNPLLYAFASRSLRHGKSDYDQIIECM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECN0</accession>
    <name>A0A3B4ECN0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Trace amine-associated receptor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECN0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033595"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024596.2">
        <property type="protein sequence ID" value="ENSPNAP00000033595.2"/>
        <property type="gene ID" value="ENSPNAG00000022320.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182934"/>
    <dbReference type="OMA" id="AIRMIIC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15314">
        <property type="entry name" value="7tmA_TAAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000030">
        <property type="entry name" value="trace amine-associated receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF415">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="30"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033595.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="321" mass="35914" checksum="BDADA08B32EB77EB" modified="2024-11-27" version="2">MPRERNVYPLAVRIVSYFAIGVIVLLTLFGNLVVIIAITHFKQLHTPTNYLTLSLAVADLLVGGAVMPASMIRSVETCWYLGSLFCKIYISLNIMLCNASLLNLVFISVERYYAVCHPLLYHSKMTPVTALFMITVCWSVAAVAGGFIFSELNVQDIEANSDVFCEGACVLVIGPMIGLALSLVSFYLPIVIMLIIYMKIYLVAQRQSRLVHNALSQVNKSRGQPTINKTERKATKTLAIVMGAFMSFWTPFSIYTLVITFYNYSGPPQLFDVLSWIAYSNSACNPIIYAFFYMWFRKALKIILFGKIVGNSSSKMQLCSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ED28</accession>
    <name>A0A3B4ED28_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">DNA replication complex GINS protein SLD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GINS4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the GINS4/SLD5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551798.1">
        <property type="nucleotide sequence ID" value="XM_017696309.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ED28"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033628"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024699.2">
        <property type="protein sequence ID" value="ENSPNAP00000033628.1"/>
        <property type="gene ID" value="ENSPNAG00000003720.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426656"/>
    <dbReference type="CTD" id="84296"/>
    <dbReference type="GeneTree" id="ENSGT00390000003246"/>
    <dbReference type="OMA" id="ILETAWI"/>
    <dbReference type="OrthoDB" id="338231at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11711">
        <property type="entry name" value="GINS_A_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21692">
        <property type="entry name" value="GINS_B_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1030:FF:000002">
        <property type="entry name" value="DNA replication complex GINS protein SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.60:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008591">
        <property type="entry name" value="GINS_Sld5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031633">
        <property type="entry name" value="SLD5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038749">
        <property type="entry name" value="Sld5_GINS_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206">
        <property type="entry name" value="SLD5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16922">
        <property type="entry name" value="SLD5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007764">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="10">
        <location>
            <begin position="78"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein SLD5 C-terminal" evidence="11">
        <location>
            <begin position="170"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="228" mass="26523" checksum="066D255752CBD09C" modified="2018-12-05" version="1">MADRLSDAGDLSEEEEEEEEEEEDVLTPAELIARLEEAWLNEKFSPELLENKSELVECVMEQLMHMEENLQRVRKGDLKASVHRMEIDRIRFVLSSYLRSRLQKIEKFFPHVLEKEKSRADGDPSFLSPEEFAFAKEYLANTEVYLKAVALRHMPPNLQSIDMLKAVPEPCLDSFVFLRVKERQENILVEPETDEQREYVVDLEEGSQHLMRYRTIAPLVASGAVQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFM7</accession>
    <name>A0A3B4EFM7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Inward rectifier K(+) channel Kir2.1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572537.1">
        <property type="nucleotide sequence ID" value="XM_017717048.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017572538.1">
        <property type="nucleotide sequence ID" value="XM_017717049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFM7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034099"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038298.2">
        <property type="protein sequence ID" value="ENSPNAP00000034099.2"/>
        <property type="gene ID" value="ENSPNAG00000023063.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438921"/>
    <dbReference type="GeneTree" id="ENSGT01030000234586"/>
    <dbReference type="OMA" id="ETQICVS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000039">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003268">
        <property type="entry name" value="K_chnl_inward-rec_Kir1.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013673">
        <property type="entry name" value="K_chnl_inward-rec_Kir_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF109">
        <property type="entry name" value="G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08466">
        <property type="entry name" value="IRK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01321">
        <property type="entry name" value="KIR11CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 13" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 13" id="KW-0813">Transport</keyword>
    <keyword evidence="5 13" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying Kir N-terminal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="16">
        <location>
            <begin position="50"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="18">
        <location>
            <begin position="193"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="12">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034099.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="439" mass="49978" checksum="A629684DE90D2EA8" modified="2024-11-27" version="2">MGSVRAHRYSIVSSEEDGMKLATIGLPNGYGNGNAGGKKHTHHQTQSRFVNKNGHCNVQFINMSEKGQRYLADIFTTCVDIRWRWMLVIFCLSFLLSWLLFGFVFWLVALSYGDLDNEEQICVSNVDSFTAAFLFSVETQTTIGYGYRYITEDCPIAVFMVVFQSIVGCIIDAFIIGAVMAKMAKPKKRNETLVFSHYATIAMRDSKLCLMWRVGNLRKSHLVEAHVRAQLLKSRTTVEGEYIPLDQIDIDVGFDTGIDRIFLVSPITIIHEIDEDSPFYDMSREELESSEFEIVVILEGMVEATAMTTQCRSSYIASEILWGHRFEPVLSVEKNDYTVDYSKFENTYEVPSTPQCSARELAEKKYIMSASNSFCYENEIALMDKEDMDVEEEHEEVKNDLFGKNLVFEGANTELVSETASSQETVLLEPRPLRRESEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFU6</accession>
    <name>A0A3B4EFU6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-aminobutyrate aminotransferase, mitochondrial</fullName>
            <ecNumber evidence="5">2.6.1.19</ecNumber>
            <ecNumber evidence="4">2.6.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">(S)-3-amino-2-methylpropionate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">L-AIBAT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ABAT</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13993"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:58655"/>
            <dbReference type="EC" id="2.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13994"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:23353"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="5"/>
    <dbReference type="EC" id="2.6.1.22" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFU6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034086"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025589.2">
        <property type="protein sequence ID" value="ENSPNAP00000034086.2"/>
        <property type="gene ID" value="ENSPNAG00000023189.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074885"/>
    <dbReference type="OMA" id="GLMCAFD"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:(S)-3-amino-2-methylpropionate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:pyridoxal phosphate binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000029">
        <property type="entry name" value="4-aminobutyrate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="10 18" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043613188" description="4-aminobutyrate aminotransferase, mitochondrial" evidence="19">
        <location>
            <begin position="34"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00015937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034086.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="502" mass="56062" checksum="A0BABF3A20211500" modified="2024-11-27" version="2">LGCPLIPDVVLCFYHFCKLVFFLSCRYVSKTAAQPVSEFEYDAPSMKTPVPGPKSKLSPPPHSLFISQNVGAVNFFCNYEESRGNYLVDADGNRMLDLYTQISSVPIGYNHPALMKAITNPNNLTAFVNRPALGILPPENFPEKLIESLLSVAPAGMTRVQTMACGSCSNENAFKAMFIWYKIKERGASGPSAEEMSSCMINQGPGCPDLSILSFMGGFHGRTMGCLATTHSKVIHKLDIPSFDWPIAPFPKLQYPLEQFVRENAQEEARCLEEVEDLIVKWRQKGKPVAGIIVEPIQAEGGDNHASHDFFIKLRNIARKHGCAFHVDEVQTGGGTTGKFWAHEHWGTGDPADIVSFSKKMLTGGYYFKDELQPDKGYRIFNTWMGDPSKNVFLSEVLNVIRRENLLEEVTRSGKALLQGLYELQAQYPHLLSRARGLGTFCAIDARDDVTRDNLILKTRDKGVLLGGCGEMSIRFRPSLVFKEYHAHQFLNIFNDVLAEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGC6</accession>
    <name>A0A3B4EGC6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGC6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038488.2">
        <property type="protein sequence ID" value="ENSPNAP00000034344.2"/>
        <property type="gene ID" value="ENSPNAG00000023557.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034344.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="95" mass="11165" checksum="404D52DDF81336E8" modified="2025-10-08" version="3">MRELVSVSYFHPERFLSLFVLCVRMHSHTCVLSCLSISKRLRTLVHVCVCISVCMYSAVQKQHTTLNVLYFQSKQPLCYLSENRCNRHLHRSPHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGK6</accession>
    <name>A0A3B4EGK6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570318.1">
        <property type="nucleotide sequence ID" value="XM_017714829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGK6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035657"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028941.2">
        <property type="protein sequence ID" value="ENSPNAP00000035657.1"/>
        <property type="gene ID" value="ENSPNAG00000003961.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437620"/>
    <dbReference type="CTD" id="570029"/>
    <dbReference type="GeneTree" id="ENSGT00940000159652"/>
    <dbReference type="OMA" id="TLMLKTR"/>
    <dbReference type="OrthoDB" id="7785529at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF176">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="162"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="430"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="446" mass="48186" checksum="8062648B7B051E4E" modified="2018-12-05" version="1">MGRLQEFSIAFTNSKVVYSPGESISGSVRVITASALQFKAIRVNCVGSCGVSSKMNDASWMVEEQYVSSMLSVADKGTLPPGEHTFSFQFLIPAHAPTSFEGPFGKVIYRVRAVIDTPRFSKDYKTQRPFYLLNMLNLNEVPDIQQPSFAVTTKKFTYLLVKTGTLMLKARSDLRGYTSGQIIKLSTEIHNKSGKDTGCVIASLIQKVTYKTKRPMFDLRTIAEVEGAGVKAGKHAEWREQIIVPPLPQSGLAGCSLIDIDYFIQVSLKSPETVVTLPIYIGNVAVNMTPSRPPPPNPLQHTPLPAMQSLSPGPVVPSAPPAEDELEGEQGAGGLASEEIPTKSHSQQDPSGQAPTMSPSAFSHAPSQALPQTQHAQSAESSAPLFCVSTGATIPFFTDGNATPVPTTCPLILPPEYSTWEYPHEPPPTYEESCSSNNSSFNTSMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EIC9</accession>
    <name>A0A3B4EIC9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EIC9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028883.2">
        <property type="protein sequence ID" value="ENSPNAP00000035625.2"/>
        <property type="gene ID" value="ENSPNAG00000025752.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282530"/>
    <dbReference type="OMA" id="WVPFAMY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000723">
        <property type="entry name" value="GPR_3/6/12_orphan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00644">
        <property type="entry name" value="GPRORPHANR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="54"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035625.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="328" mass="34422" checksum="339579E3A82CD2D8" modified="2024-11-27" version="2">FGRLILSGGSLTRAVSLRNASSPPPSVAGAADVPVSVNPWDVALCVAGTLVSCENALVIALVAHAPAPRAPAFVLIASLALADLLAGLGLVLNFAVVYAVAEPGVASLLSAGLLIAAFSASVLNVLAITVDRYLSLYGALTYHTERAATFTYAVLALAWLTSALLGALPALGWNCLEDEATCSVCRPVNKSNAAALAVSFLLVFALILQLYLQICKIAFRHAQQIAVQRHFMSASHASSTTKGVSTLSAILGTFALCWVPFAMYSLVADTRYPAVYTYATALPAICSSIINPIIYAFRNPEIQRSLRLACCGCLPYSFTSRPRTPSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EM57</accession>
    <name>A0A3B4EM57_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ammonium transporter AmtB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as an ammonia transporter. May play a role in the elimination of ammonia in the gill.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555598.1">
        <property type="nucleotide sequence ID" value="XM_017700109.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EM57"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040995.2">
        <property type="protein sequence ID" value="ENSPNAP00000037557.2"/>
        <property type="gene ID" value="ENSPNAG00000029436.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428810"/>
    <dbReference type="CTD" id="792274"/>
    <dbReference type="GeneTree" id="ENSGT00950000182844"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000001">
        <property type="entry name" value="Ammonium transporter Rh type C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF110">
        <property type="entry name" value="AMMONIUM TRANSPORTER RH TYPE C-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="12">
        <location>
            <begin position="65"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037557.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="486" mass="53630" checksum="C77C1ED753E9E646" modified="2024-11-27" version="2">MGNCIVPYCDGPKNTNVRWTLPAVCFVWQTAMVILFGAFVRYNKEVDAHWPETKIHENITSDIENDFYFRYPSFQDVHVMIFVGFGFLMTFMKRYSFGAVGFNFLIAAFGIQWALLMQGWFHHIDSTDGMIKIGVENLINADFCVASCLIAFGAVLGKVSPVQLMVMTLFEVTLFAVEEYIILNLIHANDAGGSMVIHAFGAYFGLAVSWVLYRPNLKESSQLNGSVYHSDVFAMIGTLFLWMFWPSFNSAICKHGDGQHRAAINTYLSLAATVLTTFAISSLFQKHGKLDMVHIQNSTLAGGVAVGTAAEFMLMPYGSLIVGFFCGLISTLGYIYLTPFMERKLKIQDTCGIHNLHGMPGVIGGIVGAISAAGATESVYGQQGLINTFVFKDDFKHRTPAVQGGYQAAAMCIAVSFGIGGGILVGLILRMPLWGDPTDDNCFDDEVYWEVPEEEDSADPVLQYNNHTVVNKHAEVTETGFTVEQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EPU7</accession>
    <name>A0A3B4EPU7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-1D adrenergic receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Alpha-1D adrenoreceptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ADRA1D</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564962.1">
        <property type="nucleotide sequence ID" value="XM_017709473.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000037304"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033287.2">
        <property type="protein sequence ID" value="ENSPNAP00000037304.1"/>
        <property type="gene ID" value="ENSPNAG00000028861.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434395"/>
    <dbReference type="CTD" id="146"/>
    <dbReference type="GeneTree" id="ENSGT00940000160795"/>
    <dbReference type="OMA" id="ATCQAYE"/>
    <dbReference type="OrthoDB" id="5977853at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004937">
        <property type="term" value="F:alpha1-adrenergic receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045907">
        <property type="term" value="P:positive regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000027">
        <property type="entry name" value="alpha-1A adrenergic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002233">
        <property type="entry name" value="ADR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000363">
        <property type="entry name" value="ADRA1D_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF14">
        <property type="entry name" value="ALPHA-1D ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00240">
        <property type="entry name" value="ADRENRGCA1DR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="70"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014541"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="511" mass="57429" checksum="8DE53306762CF1E9" modified="2018-12-05" version="1">MPFFKLVNESKDVLSSSEVEAFNETLDVEPFLKNRTTRRTNVTCTIVLLEPQVVAVGVFLAIFISVAIVGNILVILSVLCNRHLRTVTNFFIVNLAIADLLLGVVVLPFSASLEVLGCWVFGRAFCNIWAAVDVLCCTASILSLCIISVDRYVGVKYCLRYPTIMTERKAGVILVVIWVASAVISIGPLLGWKEPPPADESVCSITEEPGYALFSSLFSFYLPLSVILVMYFRVYVVARRTSRSLEAGVKRERGTSTDVVLRIHCRSLPEEALEQSRSRGRGQPFRSSLSVRLMKFSREKKAAKTLAIVVGMFVLCWLPFFFVLPLGSFFPSLKPSDMVFKVIFWLGYFNSCINPVIYPCSSKEFQRAFTRLLRCQCHQRRRALRRFYDQRWRTAIKGPARDSREEYQVGLPLHESCGRSSVFSCRSKGRSLGLKSWSFFPPLQKSSFHLKEKMNNLSNKIKSGAGKSSTPALGRTEIDTVAMGIYSECGEPAGYQIYDLPECYGLKETDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQM2</accession>
    <name>A0A3B4EQM2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017546459.1">
        <property type="nucleotide sequence ID" value="XM_017690970.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EQM2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037599"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041041.2">
        <property type="protein sequence ID" value="ENSPNAP00000037599.1"/>
        <property type="gene ID" value="ENSPNAG00000029438.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423575"/>
    <dbReference type="CTD" id="556154"/>
    <dbReference type="GeneTree" id="ENSGT00940000156117"/>
    <dbReference type="OMA" id="YEEIWKI"/>
    <dbReference type="OrthoDB" id="10020495at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051560">
        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282">
        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF101">
        <property type="entry name" value="MAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017353241" description="MAM domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="2">
        <location>
            <begin position="31"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="2">
        <location>
            <begin position="168"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="2">
        <location>
            <begin position="340"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="2">
        <location>
            <begin position="509"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="688" mass="77821" checksum="449C894F2885DB4C" modified="2018-12-05" version="1">MTAPLHLPSLSALLAAVLLVLVKAQLQLLPGSCNFDQDSCGYTSDPTYASWAVNNEGRFITADSSFMGEADRAVLVSPEIEILEWSCLRLVYQITGTGSLQVHLRPHGESFDYTLWMADKASDSWLIASIDLRNISRAYQLVMEARPNQGPGNSIAIFEIHIIPGYCLECNFEEHHLCGYSNQWNPNVNWYVGGSLARDPQSNLPDDHTMSNQRGHYMYVDSVYAKRFQEVAKLVSPMTTTAMSGCLSFYYQRNQARGNFFSVFTRDQLGHYEEIWRPEVYATSSWKLVQVDIKAQHPLEVVYEVAFNSPRGGYVALDDITFSPQFCHTETEPTFDPSVANCDFEESLCQYYQERVGGAVWNRVSLRPNIYRIGDHTTGSGSYLMANTRFTSRPGYVGRLHGPYLPGNHKYCLRFYYALHGFRKIDNALALYIYDEKNVAQEKIWTASEGSRDMWTEVEVTYQKPMSTKIVFVSICRNFWDCGLVALDDITVTLGDCSVTTGPLRPPPGHCYFETGDCGYTQEKKSKKGHWLRIKGHTPTSYTGPKGDHTLGVGYFMYIEASHMLHGQLSRLLSPELRGSVGPQCLTFFYHMYGSGTGTLSLLLRRGDSLKERLLWRRQGEQSISWMKATVDYDCSTRHKIIFEAIRGPSIRSDIAVDDIVFKKGPCKDPGDSISYSGFSENFNEIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQY7</accession>
    <name>A0A3B4EQY7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytoplasmic phosphatidylinositol transfer protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Retinal degeneration B homolog beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PITPNC1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidic acid (PA) between membranes. Binds PA derived from the phospholipase D signaling pathway and among the cellular PA species, preferably binds to the C16:0/16:1 and C16:1/18:1 PA species.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate(in) = a 1,2-diacyl-sn-glycero-3-phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:36435"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:36436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PtdIns transfer protein family. PI transfer class IIB subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EQY7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037709"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041169.2">
        <property type="protein sequence ID" value="ENSPNAP00000037709.2"/>
        <property type="gene ID" value="ENSPNAG00000029718.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163128"/>
    <dbReference type="OMA" id="DSQDPIM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08890">
        <property type="entry name" value="SRPBCC_PITPNC1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000011">
        <property type="entry name" value="cytoplasmic phosphatidylinositol transfer protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF55">
        <property type="entry name" value="CYTOPLASMIC PHOSPHATIDYLINOSITOL TRANSFER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="261"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037709.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="335" mass="38585" checksum="4B83BAECCAC4D3FE" modified="2024-11-27" version="2">MLMKEYRICMPLTVEEYRIGQLYMISKHSHEQSDRGEGVEVVQNEPFNDPTHGQGQFTEKRVYLNSKLPSWARAVVPRIFYVTEKAWNYYPYTITGNTHCSFLPKFSIHIETKYEDNKGCNDNIFDNELKDQEREVCFVDIAYDEIPERYYKESEDLRYFKSEKTSRGMLQEGWRDTQNPIMCSYKLVTVKFEVWGLQTRVEQFVHKVVRDVLLLGHRQAFAWVDEWIDMTMDEVREFERTIQEATNQKIGMFPPSISIGETPVSSLAPNGPASAPSTPLYTDAPEFLSVPKDRPRKKSAPETLTLPEPVQSESAPSSGSATNHASLSPAPTQME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ES14</accession>
    <name>A0A3B4ES14_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ES14"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034867.2">
        <property type="protein sequence ID" value="ENSPNAP00000037941.2"/>
        <property type="gene ID" value="ENSPNAG00000030042.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.230">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037941.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="9138" checksum="A1D24D735AE13440" modified="2024-11-27" version="2">MPCLLIMVFSASIYNVNNRGPKTALQDTMLYFHMIQSRFRMQCIDGYVRKAGTSNLYRCEEKNHSMRNNYLQCLYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IGI9</accession>
    <name>A0AAR2IGI9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IGI9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085466.1">
        <property type="protein sequence ID" value="ENSPNAP00000038215.1"/>
        <property type="gene ID" value="ENSPNAG00000033425.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230358"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001648">
        <property type="entry name" value="Immunoglobulin heavy variable 4-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="102" mass="11131" checksum="3EE77597B9C2C7B3" modified="2024-11-27" version="1">CIELTQAGSMVVTPGQSMSLTCKVSGYSVTDSSYCTNWIRQPAGKALEWVARMCGSGNAYYSDKLKSRFEISRDSSSSTVTLRGQNMQTGDTAVYYCARDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJN4</accession>
    <name>A0AAR2IJN4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TAFA chemokine like family member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAFA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554798.1">
        <property type="nucleotide sequence ID" value="XM_017699309.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJN4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070699.1">
        <property type="protein sequence ID" value="ENSPNAP00000038227.1"/>
        <property type="gene ID" value="ENSPNAG00000032323.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108428378"/>
    <dbReference type="CTD" id="100536289"/>
    <dbReference type="GeneTree" id="ENSGT00940000160682"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020350">
        <property type="entry name" value="Chemokine-like_TAFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040329">
        <property type="entry name" value="TAFA-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31878:SF0">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31878">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12020">
        <property type="entry name" value="TAFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="125" mass="13569" checksum="69C0C57A51FB0BCC" modified="2024-11-27" version="1">MQPVRVAWALAAAAAALSLLALFQAHFLREGQLVAGTCEIITLDRDSSQPRRTIARQTARCACRKGQIAGTTRASPACVDAHIVRSRQWCEMMPCLDDEGCDLLVNRSGWRCAQPGGRVKTTTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJV1</accession>
    <name>A0AAR2IJV1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJV1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047760.1">
        <property type="protein sequence ID" value="ENSPNAP00000040015.1"/>
        <property type="gene ID" value="ENSPNAG00000016375.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168744"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051:SF1">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE-RELATED PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46051">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="5"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="140" mass="16331" checksum="3C091864E1579824" modified="2024-11-27" version="1">MLGCMYYGQIGKQDTERLLEKYGKEGSFLLRDSQSVPGALCLCVRRTPFVHTYRIEHSSQGWAVETINREKPQWFQSLDELIECYRNLTPHNMVPLLYPLEKAHLSKEDRQYTGEFVLVCCHVSRSGVVMSLFFQNYCQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ILU0</accession>
    <name>A0AAR2ILU0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Suppressor APC domain containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ILU0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075229.1">
        <property type="protein sequence ID" value="ENSPNAP00000038964.1"/>
        <property type="gene ID" value="ENSPNAG00000036841.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11414">
        <property type="entry name" value="Suppressor_APC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13399" checksum="4A681E8EEDB348F9" modified="2024-11-27" version="1">WEEPGEPGGGRTCRLHTERTSILSLRITKSTPSLSHFFFCWQVKKLQELERERDSLWMGLQALEQARAWLCCRLEENKQPWVGRDVYSCESLLIQIFLFQQQVSSNKKLANKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IMY6</accession>
    <name>A0AAR2IMY6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GJC1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540089.1">
        <property type="nucleotide sequence ID" value="XM_017684600.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037400920.1">
        <property type="nucleotide sequence ID" value="XM_037545023.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037400921.1">
        <property type="nucleotide sequence ID" value="XM_037545024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IMY6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060339.1">
        <property type="protein sequence ID" value="ENSPNAP00000041053.1"/>
        <property type="gene ID" value="ENSPNAG00000034064.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108412533"/>
    <dbReference type="CTD" id="10052"/>
    <dbReference type="GeneTree" id="ENSGT01150000286949"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF6">
        <property type="entry name" value="GAP JUNCTION GAMMA-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Connexin N-terminal" evidence="11">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="12">
        <location>
            <begin position="203"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="417"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="476" mass="52538" checksum="D894EF213D14579E" modified="2024-11-27" version="1">MSWSFLTRLLEEIQNHSTPVGKLWLTTLVVFRIVLTAVGGESIYYDEQSKFICNSGQPGCENVCYDAFAPLSHVRFWVFQIILSAMPSLLYMGYAANKISHNEDVRGTGGGASAGVGAGGGYTQRRPRKMYFGARQHRPGHEDGEEEREDDPMIYEVPEIDNTRRELVPPRPKPKRRHDGRRRIRDDGLMRVYVIQLLIRSALEAAFLAGQYLLYGFRVQPIFVCSDKPCPHSVDCFISRPTEKTIFLRIMYGVSCLCLLLNVWEMIHLGAGAIRDALRKRNTAAAEDEYQLGLLGPGGVSVGVGGPALSEGETADEVGGGVREADYVGYPFSWNTPSAPPGYNIVVKPEAMPYTDLSNAKMACKQNRANIAQEEQQQYGSNEDNFPSAGDARPPPINKDVIQLEAAIQAYTLQHHANNDDDGMHDNPDNDDKPQSNIPPASQKERKHRPKHGKASSAGSTSSSSKSGEGKPSVWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWM8</accession>
    <name>A0AAR2IWM8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">REL</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWM8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041498.1">
        <property type="protein sequence ID" value="ENSPNAP00000044138.1"/>
        <property type="gene ID" value="ENSPNAG00000015394.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158732"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038061">
        <property type="term" value="P:non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01177">
        <property type="entry name" value="IPT_NFkappaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000003">
        <property type="entry name" value="NFkB p65 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000046">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF4">
        <property type="entry name" value="PROTO-ONCOGENE C-REL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHD" evidence="3">
        <location>
            <begin position="19"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="391"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="442"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="564"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="608" mass="67531" checksum="30C6A26AB0D4905F" modified="2024-11-27" version="1">HAKASLSHLCSLPPCLSLVGEPCVQIFEQPKQRGMRFRYKCEGRSAGSIPGERSSDNNRTYPSIQILNTCGKGKVRVSLVTKNDPYRPHPHDLVGKDCKDGYYEAEFGPERRVIAFQNLGIQCVRRREVRDAIMQRVNRGINPFNVPREQLLQTEEYDLNVVRLCFQVFLQDEAGRYSIALPPVISNPIYDNRAPNTAELRICRVNKNSGSVKGGDEIFLLCDKVQKDDIEVRFFTQTWEARGSFSQADVHRQVAIVFRTPAYCDTSITSPITVHMQLRRPSDQEVSEPMEFRYLPDDKDPYGCQEKKRKIDSLMKSFSSFTSGPMGPMSRTKTMAQRIKTEPTNFYGKLSSHGMMRQNQQSMYNSTYMMPSTSASPAPATFSQWPGSSLHFNTANSSPGPSNGVGMQQVNPVNPVPTVNTTGESGSSLPILSSIDLAFLDTQSPSTSQPRQEQQDQNVQHAQQPPHRKEPVATGEPLWFNYELQGAQGTQNGAPNTSGMMGMSYPYTEGQDGSEILQSLVGTQTGLHLKQEPPGQSNLTMLGPVSSSSSECTQSFTLLNYPSTNGSSQEAMRHAASSGTNGPGQRNTQTPSYSMTSMAHELWPYYPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J1B5</accession>
    <name>A0AAR2J1B5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NEK6-subfamily protein kinase</fullName>
            <ecNumber evidence="14">2.7.11.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NEK7</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.34" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J1B5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078306.1">
        <property type="protein sequence ID" value="ENSPNAP00000043616.1"/>
        <property type="gene ID" value="ENSPNAG00000002888.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156725"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08224">
        <property type="entry name" value="STKc_Nek6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000148">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000204">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000240">
        <property type="entry name" value="Serine/threonine-protein kinase Nek7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="53"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="321" mass="36936" checksum="D44AFAA2B7BD12BA" modified="2024-11-27" version="1">MVNVNINVNLLNYYSNLSFSSIHRVKQLQPEAPAVHAVHTVHTDMGYNTLSNFHIEKKIGRGQFSEVYRATYLIDSTPVALKKVQIFDLMDAKARQDCIKEIDLLKQLNHPNVIKYHASFIEDNELNIVLELADAGDLSRMIKHFKKQRRLIPEKTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRKLVDMCINPDPEKRPDITYVYDIAKHMHALTLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J1W4</accession>
    <name>A0AAR2J1W4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J1W4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045006.1">
        <property type="protein sequence ID" value="ENSPNAP00000045955.1"/>
        <property type="gene ID" value="ENSPNAG00000030215.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="111" mass="12047" checksum="3173B90314EDF7BA" modified="2024-11-27" version="1">IFPKGVAAPTLTVLPPSSVELQQGKATLVCLANKGFPSDWRLSWKVDGSSWSSGVSQSPGLLQKNDGLYSWSSTLTLQEDQWLKKTVTCEATMDSQTPVSQTLTREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J4L6</accession>
    <name>A0AAR2J4L6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017559811.1">
        <property type="nucleotide sequence ID" value="XM_017704322.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J4L6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043550.1">
        <property type="protein sequence ID" value="ENSPNAP00000044766.1"/>
        <property type="gene ID" value="ENSPNAG00000032617.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108431281"/>
    <dbReference type="CTD" id="393993"/>
    <dbReference type="GeneTree" id="ENSGT00940000155142"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04139">
        <property type="entry name" value="RalA_RalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000203">
        <property type="entry name" value="Putative ras-related protein ral-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="206" mass="23381" checksum="6BEDF7DE60D3A88B" modified="2024-11-27" version="1">MAAAKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITELESFAATADFREQILRVKEDENVPFLLVGNKSDLEDRRQVSADEAKARAEQWGVCYVETSAKTRANVDKVFFDLMREIRARKMEDSKEKNGKKKRKSLAKRIRERCCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J5T2</accession>
    <name>A0AAR2J5T2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">T-plasminogen activator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">PLAT</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.</text>
            <dbReference type="EC" id="3.4.21.68"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J5T2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054784.1">
        <property type="protein sequence ID" value="ENSPNAP00000047383.1"/>
        <property type="gene ID" value="ENSPNAG00000007521.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158930"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031639">
        <property type="term" value="P:plasminogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014909">
        <property type="term" value="P:smooth muscle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000695">
        <property type="entry name" value="Plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000152">
        <property type="entry name" value="Plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000001">
        <property type="entry name" value="Urokinase-type plasminogen activator"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026280">
        <property type="entry name" value="Tissue_plasm_act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF42">
        <property type="entry name" value="TISSUE-TYPE PLASMINOGEN ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001145">
        <property type="entry name" value="Tissue_plasm_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 18" id="KW-0420">Kringle</keyword>
    <keyword evidence="12" id="KW-0617">Plasminogen activation</keyword>
    <keyword evidence="6 19" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 19" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043310955" description="T-plasminogen activator" evidence="21">
        <location>
            <begin position="32"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="90"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="23">
        <location>
            <begin position="134"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="23">
        <location>
            <begin position="222"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="24">
        <location>
            <begin position="344"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16 17">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16 17">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="135"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="156"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="223"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="244"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (between A and B chains)" evidence="16">
        <location>
            <begin position="328"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="375"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="383"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="477"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="509"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="536"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
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            <dbReference type="PROSITE" id="PS50026"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50240"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP001501920"/>
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    </evidence>
    <evidence type="ECO:0008006" key="27">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="66453" checksum="2BA9A001EAF801E8" modified="2024-11-27" version="1">MRSSVHLRPDDIMNATVKLLLLLPLLCCTLADTERLHRERRGTRSFRDRCEDSDSAAVRDLGETWLRWRGQRVEYCRCALRGRVRCHYVPITTCYVSRCYNGGTCKEAVYSTDFICQCPPGFTGDQCEINLNEKCATGSGASYRGTWSISGSGAECINWNSTSLRGKKFTARKPEAVMLGLGNHNYCRNPDGDSKPWCYVFKKSQVMWEFCSVPNCTTAPAECVKGSGQSYRGMQVVTKSGAKCLPWDSPAISRKIYTAWRPDARELGLGSHNFCRNPDGDLSPWCHIYKGSQLTWELCDITKCPRRPSTISTLGPRAPATTNNRGSCGQRVTKPSNSLPEFRIRGGQASDIKDQPWQAAITVYRPRSRSHSFLCGGVLIDSCWVLSAAHCFHEGFTADRLRVTLGRTFRQKNSSSEQIFDVENYWLHEQYNEETYDNDIVLLKLKTEHGICAIHSPEVLPVCLPEPNLVFQDWMECEISGYGKEEEFSPFYSEQVKRGHVRLWPQERCVPEKLSGRLVTSNMLCAGDTRGLDDACKGDSGGPLVCEKDGRMMLAGLISWGDGCGKKDTPGVYTRVVKYIAWIANKMRSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J7W6</accession>
    <name>A0AAR2J7W6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J7W6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044451.1">
        <property type="protein sequence ID" value="ENSPNAP00000045936.1"/>
        <property type="gene ID" value="ENSPNAG00000030126.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45598:SF1">
        <property type="entry name" value="4FE-4S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45598">
        <property type="entry name" value="PROTEIN CBG11839-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043501731" evidence="2">
        <location>
            <begin position="24"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="451"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="516"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="586" mass="60679" checksum="4B9131CDF5C34255" modified="2024-11-27" version="1">MFFPLFAAMKCLCLSLALCLSLALCLCLALSRSVSRSRSLCVSRSLCVSRSLCVSRSRSVSRSVSRSVSLSLSLSRSLSVSVSLSLALCLALSRSVSRSLSLCVSLSLALCLARAVSLARAVSLARSVSLSLSLALCLSLSRSVSLARSVSLSLSRSLSVSLSRSLSLCVSLSLALCLALSRSVSRSRCVSRSRCVSRSRCVSRSRCVSLSLSLALCLSLALCLSLSRSVSLARSVSLSLSLCVSRSLCVSLSLALCLSLALCLSLSLARSLCLCLALSRSVSRSLSLCVSLSLALSLALSRSVSRSLCVSRSLCVSRSLSLCVSLCLSLSLSLCVSRSLSRSVSLALSLALCLSLALCLSLALCLSLCVSLALALSRSRSVSLALSRSVSLALCLSLSLALCLSLSLALCLSLSLALCLSLSLALCLSLSLALCLSLSLALCLSLSLALCLALCLALCLALCLALCLALCLSLSLALCLSLSLALCLSLSLALCLSLSLALCLSLSLALCLSLSLALCLALCLALCLALCLALCLSLSLALCLSLSLALCLSLSLALCLSLCLSLEKRTESELFTSHWPDCMSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J9Y7</accession>
    <name>A0AAR2J9Y7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017573532.1">
        <property type="nucleotide sequence ID" value="XM_017718043.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J9Y7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070788.1">
        <property type="protein sequence ID" value="ENSPNAP00000048843.1"/>
        <property type="gene ID" value="ENSPNAG00000017783.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439577"/>
    <dbReference type="CTD" id="387591"/>
    <dbReference type="GeneTree" id="ENSGT00940000155635"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd18502">
        <property type="entry name" value="BACK_NS1BP_IVNS1ABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18306">
        <property type="entry name" value="BTB_POZ_NS1BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF396">
        <property type="entry name" value="INFLUENZA VIRUS NS1A-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="643" mass="71390" checksum="EA835578A3B9C711" modified="2024-11-27" version="1">MIPNGYLIFEDESFLDSTVAKMNALRKSGQFCDVRLQVCGHELMAHRAVLACCSPYLFEIFNSDLEPHGISHVKFEDLDPEAVEVLLNYAYTAQLKADKELVKEVYSAAKRLKMERVKQICGDYLLSKMDSQNAISYRNFASSMGDSRLLAKIDCYIQEHLLEISEQEDFLKLPRLKLEVMLEDHLTLPSNGKLYSKVISWVQRSLWENGEPLERLMEEVQTLYYSADHKLLDGGLLEGQAEVFGAEDDHIQFVQKKPPRENVHRQLSSSSSGSLSPGSVKQPCKQEWKYIASEKTTNSTYLCLAVLNGILCVIFLHGRSSPQASPSATPCLQKSLSFEAKSLDEEPERLLAPMHYARSGLGTAALNGRLIAAGGYNREECLRTVECYDLKTDSWTFIAPMRTPRARFQMAVLMGQLYVMGGSNGHSDELSCGETYNPSTDEWAQVPELRTNRCNAGVCSLNTKLYVVGGSDPCGQKGLKNCDAFDPVTKEWTSCAPLNIRRHQAAVCELDGFMYVIGGAESWNCLNSVERYNPENNTWTLTAPMNVARRGAGVGIYAGKLFVVGGFDGSHALSCVEVYDPARNEWRMLGSMNSARSNAGVAVLGDLIFAVGGFDGNDFLNSMETYDPELDEWSACAEAFTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JMZ1</accession>
    <name>A0AAR2JMZ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Hypoxia-inducible factor 1-alpha inhibitor</fullName>
            <ecNumber evidence="17">1.14.11.30</ecNumber>
            <ecNumber evidence="18">1.14.11.n4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Hypoxia-inducible factor asparagine hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">HIF1AN</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-asparaginyl-[ankyrin-repeat domain protein] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-asparaginyl-[ankyrin-repeat domain protein] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54272"/>
            <dbReference type="Rhea" id="RHEA-COMP:13838"/>
            <dbReference type="Rhea" id="RHEA-COMP:13839"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:138107"/>
            <dbReference type="EC" id="1.14.11.n4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-asparaginyl-[hypoxia-inducible factor alpha subunit] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-asparaginyl-[hypoxia-inducible factor alpha subunit] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54268"/>
            <dbReference type="Rhea" id="RHEA-COMP:13833"/>
            <dbReference type="Rhea" id="RHEA-COMP:13834"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:138107"/>
            <dbReference type="EC" id="1.14.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-aspartyl-[ankyrin-repeat domain protein] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-aspartyl-[ankyrin-repeat domain protein] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54280"/>
            <dbReference type="Rhea" id="RHEA-COMP:13843"/>
            <dbReference type="Rhea" id="RHEA-COMP:13844"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138111"/>
            <dbReference type="EC" id="1.14.11.n4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-histidyl-[ankyrin-repeat domain protein] + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-histidyl-[ankyrin-repeat domain protein] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54264"/>
            <dbReference type="Rhea" id="RHEA-COMP:13836"/>
            <dbReference type="Rhea" id="RHEA-COMP:13837"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138021"/>
            <dbReference type="EC" id="1.14.11.n4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.30" evidence="17"/>
    <dbReference type="EC" id="1.14.11.n4" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JMZ1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061974.1">
        <property type="protein sequence ID" value="ENSPNAP00000051559.1"/>
        <property type="gene ID" value="ENSPNAG00000000636.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157409"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036140">
        <property type="term" value="F:[protein]-asparagine 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071532">
        <property type="term" value="F:ankyrin repeat binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036139">
        <property type="term" value="F:peptidyl-histidine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1010:FF:000001">
        <property type="entry name" value="Hypoxia-inducible factor 1-alpha inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000042">
        <property type="entry name" value="Hypoxia-inducible factor 1-alpha inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1010">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027452">
        <property type="entry name" value="FIH-1_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF105">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1-ALPHA INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="22">
        <location>
            <begin position="145"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="356" mass="41245" checksum="ECD733464831289B" modified="2024-11-27" version="1">MEMATATVVEADPTSNDGASAFSGVHSPGWTDSQLRKYPFQTRQIPRLSHTDPRAEILIDNEEPVVLTDTNLVYPALKWDTNYLQENIGNGDFSVYISENHKFLYYDEKKMSNFENFVPKSRRIEMKFSEFVEKMHQTEEQGGKERVYLQQTLNDTVGRKIVVDFLGFNWNWINKQQTKRNWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQIKGHKRCILFPPDQFECLYPFPVHHPCDRQSQVDFENPDYEKFPEFKNVLGYEAVVGPGDVLYIPMYWWHHIESLLNGGVTITVNFWYKGAPTPKRIEYPLKAHQKVAIMRNIEKMLGEALGDPHEVGPLLNMMIKGRYEQGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JN38</accession>
    <name>A0AAR2JN38_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JN38"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045978.1">
        <property type="protein sequence ID" value="ENSPNAP00000051271.1"/>
        <property type="gene ID" value="ENSPNAG00000006985.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158185"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000169">
        <property type="entry name" value="Serine/threonine-protein kinase 32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000158">
        <property type="entry name" value="Serine/threonine-protein kinase 32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF30">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 32B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="37"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="376"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="416" mass="47403" checksum="9B015DF7BA6D09F8" modified="2024-11-27" version="1">MAPPYGESSAVIAPAPDFSSKDSKESSSFFTVNFDHFQILRAIGKGSFGKVCIVQKKDTKKMYAMKYMNKLKCVERNEVRNVFKELQIMQNLEHPFLVNFWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVHFSESTVKLYICELALALHYLRNKHIIHRDIKPDNILLDEHGHAHLTDFNIAAIVKDDLKATSIAGTKPYMAPELFQPVEGTHPGYSFAVDWWSLGITAYELLRGQRPYIMRSSTPASEILQSFHKVHPYYPAALSAEIKSLLRKLLCIDAQQRLSSLSELQDMDYLSDVNWDAVLNKQVPPGFIPNRRRLNCDPTFELEEMILESKPLHKKKKRLARKTRENGGSDGSPQVILTILIFILNPHRHGENMQTPHREDPGHPAGESNPGPPRCEATAQPTTPPCHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JZP4</accession>
    <name>A0AAR2JZP4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JZP4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069742.1">
        <property type="protein sequence ID" value="ENSPNAP00000055732.1"/>
        <property type="gene ID" value="ENSPNAG00000037937.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272472"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46148">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46148:SF57">
        <property type="entry name" value="OS12G0499874 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="83"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="174" mass="19973" checksum="1CC830FCBB67F9B2" modified="2024-11-27" version="1">MVWLCTKDLRLKLPCKKLTPRFIGPLRILRQITPVSYKLELPRSYRIATTFHVSFLKPVHASQALSDVAEPPPPFEVDGSLAYAVHSLLDSRRCGGRLQYLVDWEGYGPEERSWVATEDLLDPSLISDFHRDQPDRPAPWPRGRPSHRSSLASRAAPQFLTLYNCLFNQLNCMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KB44</accession>
    <name>A0AAR2KB44_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein-glutamine gamma-glutamyltransferase</fullName>
            <ecNumber evidence="6">2.3.2.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.13" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KB44"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079778.1">
        <property type="protein sequence ID" value="ENSPNAP00000059316.1"/>
        <property type="gene ID" value="ENSPNAG00000015237.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244866"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000090">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.260.10:FF:000001">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013808">
        <property type="entry name" value="Transglutaminase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590:SF73">
        <property type="entry name" value="NOVEL TRANSGLUTAMINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11590">
        <property type="entry name" value="PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00927">
        <property type="entry name" value="Transglut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00868">
        <property type="entry name" value="Transglut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000459">
        <property type="entry name" value="TGM_EBP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
        <property type="entry name" value="Transglutaminase, two C-terminal domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00547">
        <property type="entry name" value="TRANSGLUTAMINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="9">
        <location>
            <begin position="267"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000459-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="687" mass="76920" checksum="5E13995194EF6F13" modified="2024-11-27" version="1">TEHLHRSIFQGVDLQCQMNNHAHHTEEMDTEQLVVRRGQPFSLLLQWTNGPPQPPDHQLALVLHLGKKDEVVVRMSGSRAAHGKWWFSEQAARNEVLLHVHSPADTPVGLYRLTALLLSAKGHIIEKTTSYSFYLLYNPWCAEDSVYMPDKELLQEYILNENGVLYQGSWDQITALPWNFGQFEKGVVDICFEMLDNSPAALKSSQTDITKRADPVYVSRTITAMVNANDDRGVVSGRWDGEYSDGVPPTRWTGSVPILRRWSEGGAKRVRYGQCWVFSAVACTILRCLGIPTRCVTNYSSAHDTDGNISVDYLFNEQLQSISENFHCWVESWMSREDLPKGYDGWQVLDPTPQERSDGVFCCGPCPVRAVKAGEVAIKYDAPFVFSEVNADLVCWIVHPDGKRTRASLNSGTVGRNISTKSVHGEYREDITAEYKYPEGSMKEREVYAKAGKQAAKQNERPGQLKLSIKHAQAVHGTDFDVIVEVLNVSAEDTLAHLTVISNAVTYNSLHRGECQRKTAELTVPAQKAHKEVLRLLYDNYGACISEHHLIRVTALLQTSGPLNSILQEVNIPLKMPKLHIKVTGEAVVSRKLTVIISFTNPLPVTLRRGVFTVEGAGLTEEQEIQAPGDIRPSQYVAVKCSFKPIRAGLRKLLVDFDSDRLRDVKGEAAITVQEKNVFPRTPFDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KEH6</accession>
    <name>A0AAR2KEH6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="7">6.2.1.4</ecNumber>
            <ecNumber evidence="7">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="7">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">SUCLG1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 7">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.2.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 9">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.4" evidence="7"/>
    <dbReference type="EC" id="6.2.1.5" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KEH6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077284.1">
        <property type="protein sequence ID" value="ENSPNAP00000060842.1"/>
        <property type="gene ID" value="ENSPNAG00000004480.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156351"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045244">
        <property type="term" value="C:succinate-CoA ligase complex (GDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="10">
        <location>
            <begin position="48"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="7 8">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="339" mass="35401" checksum="D775639E8B51D5EB" modified="2024-11-27" version="1">LKVTVTHAACLIPTLNLGSNSNPPQSHVFPVQQAGVRHCYTGSRKNLYISKDTKVICQGFTGKQGTFHSQQALDYGSRLVGGVSPGKGGKTHLGLPVFNTVKEAKEGTGADASVIYVPPPFAAAAIIEAIDAEIPLAVCITEGIPQQDMVRVKHRLLRQNVTRLIGPNCPGVINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCIGIGGDPFNGTNFIDCLEVFLQDPKTEGIILIGEIGGNAEENAADYLKEHNSGTKAKPVVSFIAGLTAPPGRRMGHAGAIIAGGKGGAKEKIAALQSAGVVVSMSPAQLGSTILEKRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHR4</accession>
    <name>A0AAR2KHR4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHR4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045140.1">
        <property type="protein sequence ID" value="ENSPNAP00000063868.1"/>
        <property type="gene ID" value="ENSPNAG00000035016.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164983"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF236">
        <property type="entry name" value="PROTOCADHERIN GAMMA-C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043557555" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="26"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="687"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="23"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="130"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="239"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="355"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="452"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="576"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="845" mass="92517" checksum="00001B17379D117F" modified="2024-11-27" version="1">MRKMRRKWWIAGLLQALAVWNIIEAQIRYTIPEELNEGTVVGNIAKDLDLKLSEVFERNLRLASESGKQYFGVDSAKGELVVSERIDRETLCSQSATCLLPLEAIVENPLQLYRVEIEIQDINDNSPVFQNNEKVLNIAESTAPGARFRLEAAQDPDVGSNSLRTYTLSKNEHFALNLKSAKDGSQVPELVLEKPLDREKQSTISLMLTALDGGKPARSGTAEITVRVLDNNDNAPVFEKAQYDAQLAENTSPGAVVVTVKAQDLDDGLNKEISYSFGPHTPDVLHRLFSVDSVTGEVKVKNEIDFETDQSFDVDIVARDQGTPPMEGLCSVHLEILDMNDNAPEIVLKSLPSPVREDAPVGTVVALISAKDLDTGENGKVSLTLSPGAPFSLKPSISNHYTLITDQKLDRESHSVYNVVIVAADSGSPQLTSKQEIVVSVLDVNDNPPIFTQPFYSVYVKENSAAGSILCSVSASDPDQGENAKVSYSVLDSRVLDTPVSSYIYINSDNGSIYTMHSFDYEKMKVFQIQVQAKDHGSPSLSSNATVHVFILDQNDNAPAVIYPSAVMGSASHQRMPRSAKAGHLVTKVTAVDADSGHNAWISYRLAEAADASLFSVNLHTGEVRTKRSVSEQDDSSQRLLIEIKDNGEPVQSTTVTVDVLIEDGFHEPVSDYRKKSAEVSKKSGKITLYLIISLASVSLLCLMTFFILLVKCARGSRGSSSCCIRRSESGYKNPNRNLQIQLNTDGPIKYVEVLGGDMMSQSQSFGSYLSPMSEFSDFTLVKPSSTTDFTDTLNVLDASLPDSTWKFESQQLEIRKTNHCGISIKNNFSGFTSESSFMVGQSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KMB8</accession>
    <name>A0AAR2KMB8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KDELR3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPI-coated vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Endoplasmic reticulum membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578151.1">
        <property type="nucleotide sequence ID" value="XM_017722662.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KMB8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064035.1">
        <property type="protein sequence ID" value="ENSPNAP00000065440.1"/>
        <property type="gene ID" value="ENSPNAG00000009060.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442565"/>
    <dbReference type="CTD" id="11015"/>
    <dbReference type="GeneTree" id="ENSGT00390000004010"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="215" mass="24942" checksum="96C4FC02B691A4A9" modified="2024-11-27" version="1">MNVFRLAGDVSHLVAIIVLFLKIWRSRSCAGISGKSQVLFALVFTTRYLDLFTSFISIYNTVMKVVFLVLAYATVYLIYMRFRNSYDSENDSFRVEFLLVPVAGLSFLENYAFTPLEILWTFSIYLESVAILPQLFMITKTGEAESITTHYLFFLGLYRALYLANWVWRYHTEGFFDQIAVVSGVVQTIFYCDFFYLYFTRVLRGSGKMSLPMPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNT7</accession>
    <name>A0AAR2KNT7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MYH2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000087570.1">
        <property type="protein sequence ID" value="ENSPNAP00000065983.1"/>
        <property type="gene ID" value="ENSPNAG00000033577.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164626"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000004">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000006">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000013">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000007">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF44">
        <property type="entry name" value="MYOSIN HEAVY CHAIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9 13" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="16">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="15">
        <location>
            <begin position="87"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="13">
        <location>
            <begin position="653"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1584"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1882"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1587"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1919"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="180"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1962" mass="224860" checksum="1D00E4CD0642DDE5" modified="2024-11-27" version="1">MSTDAEMAVYGKAAIYLRKPEKERIEAQSRPFDAKTACYVADQKELYLKATIKSRDGGKVTVEVLTTKEERTVKEDDVSPMNPPKFDKIEDMAMMTHLNEASVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGEKKKETAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLSDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFSNEEKMSIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKVAYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNAVSALAKSIYERMFLWMVVRINQMLDTKQARQFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNIAGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPEGQFIDNKKACEKLLGSIDIDHDQYRFGHTKVFFKAGLLGTLEEMRDEKLAALVTMTQAVCRGYLMRREFLTECQESIYTIQYNIRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFTKCKEDLAKAEAKKKELEEKMVSLLQEKNDLQLQVASEAENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFEISQFLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEEATLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKTKNDENLRQINDLSAQKARLQTENGEFGRQLEEKESLISQLTRGKQAFTQQIDELKRQIEEEVKAKNALAHGLQSARHDCDLLREQFEEEQEAKAELQRSMSKANSEVAQWRSKYETDAIQRTEELEEAKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALASNLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARALSTELFKMKNSYEEALDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKAKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRVTESMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQGQLKDAQLHLDDALRGQEDMKEQVAMVERRNTLMLAEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQVQSEVDDTVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELEAEVEGEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQAEEAEEQANAHLSKLRKVQHDLEEAEERADIAESQVNKLRAKSRDSGKDPGRPAGETNPRPPRCEATALPTTPPCRPCRYFLKCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KSX2</accession>
    <name>A0AAR2KSX2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tropomodulin 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMOD1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037388599.1">
        <property type="nucleotide sequence ID" value="XM_037532702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KSX2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070222.1">
        <property type="protein sequence ID" value="ENSPNAP00000067375.1"/>
        <property type="gene ID" value="ENSPNAG00000032768.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108443441"/>
    <dbReference type="GeneTree" id="ENSGT00940000158695"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000006">
        <property type="entry name" value="Tropomodulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF8">
        <property type="entry name" value="TROPOMODULIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42721" checksum="099E3F1FB8591488" modified="2024-11-27" version="1">MSFRKEMEKYRDVDEDELLQKLSEEELRRLEDELEELDPDNALLPAGLRQRDQTKKAPTGTFHRDNLLAHLEKQAKEHPDRDDLVPYTGEKRGKPWIPKTKVVDPILEDVTLEPELEEALANATDAELCDIAAILGMHTLMSNQQYYEALASSTIVNKQGLNSVIQCTQYKPVPDEQPNDTDVEETLLRIKHNDPDLVEVNLNNIRNIPIATLKAYAEALKNNTVVERLSIVGTRSNDPVAHALADMLHVNTALRSLNVESNFITGAGIIALVDALQHNSTLQELKIDNQSQPLGNKVEMEIASMLEKNSTLLKFGYHFTQQGPRLRCSNAMMNNNDLVRVVRTDSDGAITFTLSVPELERAFCKKFKFSSKPNKKEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KTY8</accession>
    <name>A0AAR2KTY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin-ribosomal protein eL40 fusion protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 60S subunit of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KTY8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057647.1">
        <property type="protein sequence ID" value="ENSPNAP00000067798.1"/>
        <property type="gene ID" value="ENSPNAG00000030897.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153593"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000014">
        <property type="entry name" value="Ubiquitin-60S ribosomal L40 fusion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.50:FF:000001">
        <property type="entry name" value="ubiquitin-60S ribosomal protein L40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="13">
        <location>
            <begin position="6"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="133" mass="15311" checksum="3CC5DD4598614073" modified="2024-11-27" version="1">MLHSKMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLKQLANKYNCEKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KYW4</accession>
    <name>A0AAR2KYW4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SPPL2A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A22B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KYW4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071522.1">
        <property type="protein sequence ID" value="ENSPNAP00000069455.1"/>
        <property type="gene ID" value="ENSPNAG00000027974.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412680"/>
    <dbReference type="CTD" id="84888"/>
    <dbReference type="GeneTree" id="ENSGT00940000157722"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF34">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043355581" description="PA domain-containing protein" evidence="10">
        <location>
            <begin position="21"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="11">
        <location>
            <begin position="55"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="532" mass="58238" checksum="0D741464E106E3B8" modified="2024-11-27" version="1">MAAHIWIIVAAVLFWNKGECQEAVLHVSNGALHKDYCLVYNSSWSNISDSLSDAVKYELVNLTYSMLCDEGTMKPGSLDGKAVAVMRGGCAFSRKAQVAQDLGAAVLLITSKEEMTTPSANHTEYGKVKIPLALMRYSDFLDAQKVFSDKMEVQLFAPPIPPFDVSIIVMLALAVFTIAMGGFWSGAVERAKQNAGPSLSGGEERADGGDVMLYSPLKVLLFVVLMCGMLVLMYFFYKWLVYVIIVIFCLASATALYSCLNALSNAVGCSKLNVSCGERSCSVRSLLLAAVCITLAVVWGVYRNEDRWIWILQDLLGVAFCLHFMKTITLSNYKICVFLLSLLLIYDVFFVFITPFLTPNGESIMVQVALGPGAGGEKGDGAVVIPADPSPSYEKLPVVMRVPRFSAWAQNLCMMQFSILGYGDIIVPGLLVAYCHRFDVWIGSNRIYFISCTIAYCVGLAVTFAVMLLSRMGQPALLYLVPFTLITSAAVALSRKEFRQFWAGTTYQVLDSSREPLIPDGEAAYPSSALKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L5N0</accession>
    <name>A0AAR2L5N0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">KASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus outer membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nesprin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L5N0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044326.1">
        <property type="protein sequence ID" value="ENSPNAP00000069952.1"/>
        <property type="gene ID" value="ENSPNAG00000018077.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154656"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000157">
        <property type="entry name" value="Nesprin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000126">
        <property type="entry name" value="Spectrin repeat containing, nuclear envelope 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012315">
        <property type="entry name" value="KASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056887">
        <property type="entry name" value="SYNE1/2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF4">
        <property type="entry name" value="NESPRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10541">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25035">
        <property type="entry name" value="SYNE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01249">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51049">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="766"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="806"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="9">
        <location>
            <begin position="834"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="KASH" evidence="13">
        <location>
            <begin position="804"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="400"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="671"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="863" mass="100023" checksum="1582EFA29D34C4AB" modified="2024-11-27" version="1">MRDFQSGTVCMCNFLISDLFVFRVNKLKETMVTVQQLDKNMSNLRTWLSRIEAELAKPVHYSICHGDEIQKRLAEQQELQRDIEQHTEGVASVLTLCDVLLHDEDACSSDSENDSIQLTTRSLDQRWRNICSMSLERRMRIEETWRLWCKFQEDYSRFEDWLNLTERAVAEPKSSDVLYTVAKEELKTYEAFQREVLEKLTQLEIINNQYRRLARENRTDTASRLRAMVHQGNQRWDMLQRKVAAVLRRLRHFTAQREEFESTRESLLVWLTEMDLQLTNVEHFSESDIHQKLKQLNGFKKEITLNTNKIDALIVFGESLIQRSSPLDAALIEDELEELHSYCQEVFSRVARFHQRLTSPRPVQEPDLCGTESTAETEGSNLAEVSQNISQPSMCFLAPPQERSGRETPVSVDSIPLEWDHTGDVGGSSSHEEDEDSSFFSSLSGNYICCCFHQHIFFTITLRLCIHSNIRSVKKVSLILDEEQQQEDHGLTGLTAADKQSGVIERWELLQARAVSEEQCSSRDPRKLTSDLLNITSWLGRVAPELERLQKAETSVSVEIMEARVKELKEMQKAFARYKTLMLSLNLGGRELQCEASVEMQQLQEGLRNMNQGWTQACMGLEGWEDSLRNSLMRCQEFHEMLHSLLLWLAHAESRRYTINILDPSVPPSKLQEHRTALMCLEDELKNRQKQVGSLQEIASELLPESGEDDNSEAREKLHVIGIKLRLLLRQVRQDLQTVQERLVRKKCRFKIVAKSGTESTRLQRWERWRCVGVCVGVCAVWYFWFACKRSNLSERSLERMMGRSFFVRVLRAAFPLHLLFLLLLVLACLVPLSEQDYSCTLSNNFARSFYPMLRYTNGPPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L8R4</accession>
    <name>A0AAR2L8R4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L8R4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071015.1">
        <property type="protein sequence ID" value="ENSPNAP00000072983.1"/>
        <property type="gene ID" value="ENSPNAG00000037894.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156074"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000002">
        <property type="entry name" value="Cyclic nucleotide gated channel alpha 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000030">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032406">
        <property type="entry name" value="CLZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF20">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CHANNEL SUBUNIT ALPHA 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16526">
        <property type="entry name" value="CLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="372"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="558"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="527"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="626" mass="73643" checksum="D287F8C625EFB152" modified="2024-11-27" version="1">KEEKEQENEKEKEKEKEKQEKEKEKEKEKEKEKEKEKEKDKKEGPKEMFVINPDSNLYYQWLLLITLPVMYNWTMIIARACFEELQHNYLITWFILDYLCDLLYVADMVFRTRTGYLEQGLLVKDKKLLLKHYTDSFQFRIDVISMIPTDVLYFALGLDYPEIRINKLLRLNRMFEFFDRSQTMTNYPNIFRICTLVMYIIIIIHWNACIYFSFSKSIGFGSDPWVYPSLDEPEFGDLSRKYSFSLYWSTLTLTTIGETPSPELDSEFFFHVFDFLVGVLIFATIVGNIATMISNMNAAQAQFQARIDNIKQYMHVRHVSKDLEKRVIKWFDYLWTNKKAQDEREVLRYLPDKLRAEIGMNVHLDTLKKVRIFADCEAGLLIQLVLFLRPQVFSPGDYICRKGDIGREMYIIKEGKLAVVADDGVTQFCVLGDGSYFGEISILNIKGSKAGNRRTANIRSIGYSDLFCLTKDDLMEALTEYPEAKTMLEDKGRQILLKDGLIDLDPANLKPDQKDLEDRVNRIYSTMMLMQTKLKKLLENYDKTEKDLKQRIAQIERYAGEEVEEDEEEEQEGQKEEEAAPEQEKEEETKDEQKDGEDKPEEEEKPEEEKKPGEEEKPEGEEKPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBS8</accession>
    <name>A0AAR2LBS8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBS8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074927.1">
        <property type="protein sequence ID" value="ENSPNAP00000074053.1"/>
        <property type="gene ID" value="ENSPNAG00000037313.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000250120"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8962" checksum="FE298F739412D2C1" modified="2024-11-27" version="1">MNSLQYQEILEENVMESVTNLRLGRHWTFQQDNDPKHTSKSTRAWLKMKVKYNNSGKMSGPKFLHSAVKDSVPVIANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LH74</accession>
    <name>A0AAR2LH74_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Teneurin transmembrane protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000066470.1">
        <property type="protein sequence ID" value="ENSPNAP00000073741.1"/>
        <property type="gene ID" value="ENSPNAG00000030780.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234566"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000016">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000021">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000005">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000006">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022385">
        <property type="entry name" value="Rhs_assc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009471">
        <property type="entry name" value="Ten_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057629">
        <property type="entry name" value="Teneurin1-4_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03696">
        <property type="entry name" value="Rhs_assc_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF7">
        <property type="entry name" value="TENEURIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23093">
        <property type="entry name" value="GBD_Tenm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06484">
        <property type="entry name" value="Ten_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23538">
        <property type="entry name" value="Teneurin_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51361">
        <property type="entry name" value="TENEURIN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 15" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043647343" description="Teneurin transmembrane protein 1" evidence="18">
        <location>
            <begin position="19"/>
            <end position="2648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="831"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="859"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="891"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="domain" description="Teneurin N-terminal" evidence="20">
        <location>
            <begin position="79"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="594"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="658"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="19">
        <location>
            <begin position="698"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="679"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="702"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="723"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2648" mass="290968" checksum="3F034CFD0B51EDB4" modified="2024-11-27" version="1">LSLSLSLSLSLPLSLSLSLSLPPSLSPSLTPSLSLSLSLSPSLPLSLSLSLSLSLSVSLCLSVSVCGASVGMASDPESEGGASPDHALRLWMQEVKSERTSCVSSRANSVLSLTDTEQEGRGDLYSLYFSPLRCQVPPADPAVPVARFSFRPLPPPPPPPHACTCVRPAPSAAPELIQRNTLPARGHQSDLTKTAQDDGGQSENTWGLNSNIPLETRHFLFKHGSGSSTLFNATNQNYPLTSSTVYSPPPRPLPRTPLSRPLFSFSKPYRCCNWKCTALSASAVTVTLALLLTYVIGESFSVALISVAERLRRMTVSRVFFGAGRNDPSQRGRGIDRGEVDIGTQLVQVIPPGQFWRFSVVVQHPEYIRFNISHTHNALLGIYGRRNLPPTHTQFDFVKLLDGKLKQEFWNPDIPKVSASNGLLSALQETVFMEYLDLGTWHLAFYNDGRKMEQVLVHSTAIEMMDGCSTNCNGNGECVAGHCHCFTGFLGPDCSKDSCPVLCSGNGEYEKGVCVCRVGWKGAECEVEDGQCIDPTCSNNGECVDGSCVCTPAFKGVNCEEVDCVDPTCSGRGVCVRGECVCSAGWGGEVCETPLPACKEQCSRHGTYHPQTGACVCEQGWTGKDCSTEVCPLPCSAHSVCVGGQCQCEDGWEGPVCDKQACHPVCEEHGECKDGQCVCHPGWEGEHCTIAHYLDLLDKDACPGLCNGNGRCTLEQSGWHCVCQSGWSGPGCNIVMETQCNDSGDNDGDGLVDCVDPDCCSQPVCVNSPLCQGSPDPRDIIQQIHAPFAPRPSQQFFERVRFLIGRDSTHTLPGDLPYDSSSNKINIQNRILISLSLSLSLFLFLSLSLSLFLSLSLAYSLSLSLSLSLSFSLSLTYSLSLSLSLSFSLSLSLSLSLILSLSLFLSLPLSLSLSLSLSPSVQAVQEEIEIAGSFLKLSYLSTRSPGYRCLLRVILTYAVLPVGLAKVHLSVSVEGTLVTKWFPASPNLVYVFSWNKTDVYGRQVTGLVQAQVSVGFEYESCPDFIIWQKRTVQLQGFEMISSHLGGWALDKHHTLNLESGILHKGDGENVFISQQPPLISTVMGTGAVRPIACPSCNGPAADRKLFAPVALACGSDGSVYVGDFNFIRRILPNGFTISVLELRNRDIRHSTSPAHKYYLALDPVSEAVYVSDSSSRRILRLKAVTEPRDLSRNAEVLAGNGEQCTPFHPNQCGDGGKATDASLINPRGITVDKYGSVYFVDGTTIRKIEKGIITTVMGSNGLTSTQPISCDTHMDISQVRLEWPTDLAVSPLDNSLYVLDNNLVLQVSEAGLVRVVAGRPIHCPLPVTEPPAGSASTQSALEGAKAISVSHQGTLYIAQTDDRKLSRILEVNTNGEMSVIAGAVSECDCKIDPDCDCFSGDGGYARDAKLKSPSALAMSPNGTLYIADLGNVRVRSLVARRPQLTSDLLYEVMSVAQQEVYLFAPNGTHLFTRCLVTGDYLYNFSYVGAGLVAAVTASEGGVVQVRRDSNGSPLWLVTPGGQVYWLTLSTAGTLRRVSALAHDLAQISYYGNTGLLATMNPLLILMYNSDGHLINVTLPTGEVSSFHGDMGKSVRVEASTSNRENFITITNHSEENTVYTLRQAHSVSVYRVSSDGSLWVTYASGMDVTLSTEPHLLPGTLPGVSPGAVHPTMGRCNITLPGEQSHSIIEWKQRREQARNYSTYERRLRAHNRNVLSIDYNQASRVGKIYDDHRKFTLRLQYDDRGRPVLWAPSKFSDVRVSYTSDGLLASIQRGNWSERRDYDNGRLITRTWANGKTWTYSFQEKSIQLLLHSQRRYTFEYDQTDYLMSITLPSMVKHGLQTALSIGYYRNTYTPPDSPGHALVQDYSPDGRLLQTLYLGSGRRVIYKYSRTSRLSEILFDSTLVSFTYDESSGAVKTVHLMQEGFVCTIRYRQTGPLVGRQIYRFSEEGLVNARFDYSYNNFRVTSMQAMINETPLPIDLYRYVDVSGRIEQFGKFSVISYDLNQVITTHAMKHSKIFNPNGQVVEVQYEILKSIAFWMTLQYDSLGRVANCDIRIGVDGNITRRSYEYDADGQLQSVAVDDRPQWRYSYDLNGNINLLSHGNSARLTPLRYDLRDRITRLGEIQYRTDDDGFLRVRGNVVFDYTSNGLLAGALDRDTGKRLWYRYDGLGRRVSSRASDGTLLQFFYADLTEPTRVTHLYNHTASEITSLYYDLQGHLIAMEMSSGQEFYIACDAAGTPLAIFSSRGQVVKELRYTPYGDVYRDSNPSFQLPFGFQGGLYDSLTRLVHLGRRDYDVVAGRWTTPNHDLWAEASMDPKPFNLYAFKNNYPLGGLQDVTKFTRDISSWLQLFGFQLHNVVPGFPKLPVENTEQTYEMMNVQSRTHSWDPSKIVLGIQCELQKLLQNFISLDRLPMTPANGRPGGRRGRSPRFSALSSIFGKGVKFAIRADGAVSAEIIGVASEDSRRIAAVMNGATYLRGLRFTLDGRDTHHFTKDSPLEADLGVVWGGSGAAGGGARVLENGVNVSVSQMSAVVAGETRRFADLVLQQGALCFNIRYGATPDEERTRVLEGARLRAVQGAWLLEQRRVKDGEGGARVWANKEREELLSVGHVSGYEGFYVLPVEQHPELADSPFNIQLIRQTDAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIQ8</accession>
    <name>A0AAR2LIQ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LIQ8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065968.1">
        <property type="protein sequence ID" value="ENSPNAP00000074654.1"/>
        <property type="gene ID" value="ENSPNAG00000030366.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165062"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF854">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="40"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="315" mass="35619" checksum="FA1DE36995DC5DBD" modified="2024-11-27" version="1">MDFNFSIYTSLTLESLELPPSNVFPAFIFGTLTYCAILVFNMTVLLTIALNRKLHKPMYVLLFNLPISDMVGASAFFPQLVSSILSQNRSITYSACFVQALLVHLCGTGAILTLSAMAYDRYIAICCPLKYNTLMSPNNLLKIIIMIWILDFTLIGSLLALNYRKEICSTKIVDIFCNNPSLMKLVCGDTKLNNYYGLFLTAFFEVVFTYVQILITVVVKRQSDARSKAIQTCGTHLIVFLCFEFNTFFAFIAHRFETASPSLRRALGASAVIFPPILNPLIYGLKTKEIRENFILFFHKVFPQKQKNKLVGVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLI2</accession>
    <name>A0AAR2LLI2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 18">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="3 18">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Binds 3 Ca(2+) ions per subunit. Two of the Ca(2+) ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="3 18"/>
    <dbReference type="RefSeq" id="XP_017566863.2">
        <property type="nucleotide sequence ID" value="XM_017711374.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLI2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086033.1">
        <property type="protein sequence ID" value="ENSPNAP00000075624.1"/>
        <property type="gene ID" value="ENSPNAG00000028052.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435492"/>
    <dbReference type="CTD" id="337489"/>
    <dbReference type="GeneTree" id="ENSGT00940000158392"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13363">
        <property type="entry name" value="PH_PLC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08593">
        <property type="entry name" value="PI-PLCc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000088">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000058">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000022">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028391">
        <property type="entry name" value="PLC-delta1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF199">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 18" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="10 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="20">
        <location>
            <begin position="44"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="23">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="22">
        <location>
            <begin position="511"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="630"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="468"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="540"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="567"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="671"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="695"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="724"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="725"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="726"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="O-linked (GlcNAc) serine" evidence="17">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR628391-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR628391-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR628391-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR628391-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="776" mass="88732" checksum="ADE3421E7290DA83" modified="2024-11-27" version="1">MQCIRKETSRSKSEELVHQAQSLRVASLNSKLLGMEDDPDLQFLLSGAGLVKVRSSSWKKTRFFKLQEDCKTMWHESKKTLRSKQTFSVEDIDSVRSGRQTEGLKKYTEEALDPRVFSILFKGRRKNLDLIANSEGEAKHWVSGLEKVIFNLSNLSQQSKTEHWIYNCLRKADINCDNKMSPQELINFLHDINIEVDDEYVQMLIEKCDQSKCGSLEGEEIQHFYEILTQREEIDVIYGAYAKTEGMLSAANLLDFLQKEQKEQVDLSYAAQLIEKYEVDETAKAKQQMTKDGFLMYLHQPEGLIMNPAHKRVYQDMQQPLNHYFISSSHNTYLMEDQLKGPSSTEAYIRALMKSCRCVELDIWDGSDGEPVIYHGYTLTSKILFKDAIKAIKEYAFKTSEYPVILSLENHCSVEQQKLMAHHMTSILGSALVTKPLGDHMPTELPSPEDLKRRFLIKGKRLNKLEASLSDQASAEEEESVTEEEDCRDDEEESEKNLSGKSKTLKLAKELSDIVIYCKSVKFQGFEHAQTHQAFYEMCSFKEGDAFKFAEKSAFEYIHHNVDKLSRIYPSAFRTDSSNYDPVPLWNAGCQIVALNFQTPGPEMDLNQGLFGQNGLCGYVLKPSYLRDKASEFDPITLTRGPWLKHKELHVMVISAQQLPKVSQKMASVVDPLVRVEIHGVPADETEKETRHIQNNGFNPMWNENFQFDLYVPELALVRFVVEDYDAVSNNDFIGQCTVPFTSLQNGYRHVPLFNENGDLLSSAGLFVHTMVVNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LUK4</accession>
    <name>A0AAR2LUK4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CMKLR1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LUK4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080851.1">
        <property type="protein sequence ID" value="ENSPNAP00000080255.1"/>
        <property type="gene ID" value="ENSPNAG00000034054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230438"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF0">
        <property type="entry name" value="N-FORMYL PEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMETLEUPHER"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
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            <end position="66"/>
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            <begin position="274"/>
            <end position="297"/>
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            <begin position="58"/>
            <end position="295"/>
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        <location>
            <begin position="324"/>
            <end position="343"/>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="Proteomes" id="UP001501920"/>
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    <sequence length="343" mass="39212" checksum="42A891B72BCC0478" modified="2024-11-27" version="1">LNRNTLDCLDGDIMNSTQVAEIKNDTMSQTSRSSSCKDAVCIFYAIAEVIICVLGIAGNGLVIWITGYNVKKTVISTWYLSLAVSDIIFCFTLPFSVVFVVKKAWPFGLFMCKFRSFILWLNMYSSIFLLVIISVDRCVLVMFPVWAHNKRTIRKASVIVVFAWIIAAVCSTPSAVFRVIKHSTYKKTTCSYKYSYRKNYIATVTCRFVSSFVIPFLIIFICFVVILQKLKSNHRVRFKKPFKIMTVLIVNFLICWLPYHTLLLIQLHKYKLSIAIQRFCFILVSANSCLNPFLYAFMGKDIKEQCYAFRLKIENAIKEEEDQNTSQGTAIANSGESQHSTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LY54</accession>
    <name>A0AAR2LY54_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF236</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000068276.1">
        <property type="protein sequence ID" value="ENSPNAP00000079261.1"/>
        <property type="gene ID" value="ENSPNAG00000017138.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441788"/>
    <dbReference type="GeneTree" id="ENSGT00940000156787"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Putative zinc finger protein 236"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002349">
        <property type="entry name" value="Zinc finger and BTB domain-containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000264">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 236"/>
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    </dbReference>
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        <property type="entry name" value="zinc finger protein 236"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 236"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 236 isoform X2"/>
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        <property type="entry name" value="Zinc finger protein 236 variant"/>
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        <property type="match status" value="25"/>
    </dbReference>
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        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF21">
        <property type="entry name" value="ZINC FINGER PROTEIN 236"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="28"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
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    <feature type="domain" description="C2H2-type" evidence="14">
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1868" mass="205950" checksum="54065BF15A059E65" modified="2024-11-27" version="1">MTECFYDNTASHHSFFILTKIKDGSDELKAETFCGVEYRCNICSQIYSTENQLQRHHRDHEANDKPHRCDLCPQSFNVEFNLRLHKSTHNTSDPTCPVCDKKFSRVASLKSHIMVHEKEENFLCSECGDEFILQSQLSLHLEEHRKELSGTKVYTCRTCSKEFTSATLLKEHMKTHIRIRPILSSLRNYKKNIDRSGFTNTCHHCGKTFKKPSQLVRHIRIHTGERPFKCTHCGKAFNQKVVLQTHMVRHTGEKPHLCMLCPASFSQKGNLHSHVQRVHSESSGGPVYPCMDCSCVYKKLGSLNAHISKMHISIMEVEEPASTQDGVESGQGGAAEAAEGVTDVIQQLLELSEQVTGDHAQAPPPGPTITMETAINQDILQQALENSGLSAIPVQPDTQTSAGSRPETETATPATGCTDTPSQKPAVPQKLLKKEKRSIFKKPIQMPGTIREERGVRWHGCPYCSKEFKKPSDLVRHIRIHTHEKPYKCKQCFRAFAVKSTLTAHMKIHTGIKAFQCQYCMKCFSTSGSMKVHMRLHTGVRPFPCPHCDKIFRTSGHRKTHIASHFKNIQQKKHKFPRKPHRARAAKSSLPLPDIPLQEPILITDLGLMQNQNPRAALQQYLEMVDNDRPYKCTFCSKAYKKSSHLKQHVRSHTGERPYKCVQCSKGFASSGVLKAHIRTHSGLKAYKCLLCDTTFTTNGSLRRHMTTHSDIRPYMCPYCQKTFKSSPNCRKHMKTHRFELAQQLRQQGSAEAQPLEDPMGVAGTVEDQMGGTNSVKDPMSSANTVEDPMSGTRTVAHDLQVEMQVGPTGLTQPTSAMGAEPQAMLQVVGGVQQVALQTQLGEGTLVQDADTFVSTQHISQYETPALPQPAYDQPALTQAFSISEGFSQQPSLSAVQQLQDSSTLESQALTSTYHTQSLLTTETAGLLQESTEGQLHLIRQTQQYQDEEESNRRAYRCSMCDKGFKKSSHLKQHVRSHTGEKPYRCNLCGRSFVSAGVLKSHLNTHTGVKAFKCNVCDSSFTTNGSLNRHMIIHLNTKPFKCSLCEESFRTHLLRRKHMKLFHAIGPRVLDGEEACEEDSGAEHSVMKRNRAGIITFTEEQTAELAKRDPGDDASVSEKVLVQSAAERDRISEMKDKAVVLDLEPKFANRCQYCPKSFKKPSDLVRHIRIHTGEKPYKCDECGKSFTVKSTLDCHVKTHTGQKLFNCHMCNTSFSTKGSLKVHMRLHTGSKPFKCPYCDLRFRTSGHRKTHIQCHLRQGIEGRKMRRGGERTAGSASQNQPQGQAPSGQQGSAAPETLPPVGLLHTAPTDPSIYIPTNPVLTGQYEQTLLQQGIVGQAILPATMSAGGDLTVSLTEGLATLEGIQLQLAPASLLCPNVQISGIDTTNINNITLQIDSSILQQTLQQSGLLSHTLSGDSQTHSATHLLATTGEASVPANVVIQPVNSLPLQNPPTPASVAIGNLTEQDNTGSEQLAQVVGGGAGGPEITLTISNSSLTQALTHPHTHSANTPASSNQEITLTISGQDLLAQSCGNAGAEVSGALRLTTAHTSVSSEQLLPHSPAPAGMAVTTLAPSTSLPQNLVMSSAGVASDGSVTLTLADTQMLDTVTLNLNSQGQQFPAVTEQGSAVQPANNTQQVILVGHPAENTPHQEGLKIVEGQAEVKGSEVDVQLNQCFYCSQTLPTANALRRHCRTMHGKERCHVCRVCSKAFKRATHLKEHEFVHLNGPTLSSQKPRVFKCSSCDKAFAKPSQLERHIRTHTGERPFKCSQCDKAFNQKSALQVHTVKHTGEKPYKCEVCTISFTQKSNMKLHMKRSHGYGKQTDAERDVGREQECVPEPPQATSSRDETSAELEPAGDWQCPIPSVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZ47</accession>
    <name>A0AAR2LZ47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Perforin-1-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552884.1">
        <property type="nucleotide sequence ID" value="XM_017697395.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LZ47"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045267.1">
        <property type="protein sequence ID" value="ENSPNAP00000079611.1"/>
        <property type="gene ID" value="ENSPNAG00000019701.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427337"/>
    <dbReference type="GeneTree" id="ENSGT00940000164067"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140911">
        <property type="term" value="F:pore-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04032">
        <property type="entry name" value="C2_Perforin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037300">
        <property type="entry name" value="Perforin-1_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF3">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043714571" description="Perforin-1-like" evidence="9">
        <location>
            <begin position="30"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="11">
        <location>
            <begin position="27"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="393"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="592" mass="66312" checksum="2BBE57F4A6182812" modified="2024-11-27" version="1">MLQAITLRVMLAASFPLLTIPACFKGTSAQCQDAEFAPGSDLAGEGFDITTMQRKGAFVIDMSVWMLKNETCTLCRNPYMEGKKQKLPLSVVNWRPSKKCTMKLSSSVYQSSESLLSSSTSAIDNNWKVNLGIIDIRGKGSLMLAGTNSKLAQYSMEKTKKDKFSFTSHAVSCGYYSYRVSSRPAVHPELTYEIDRLPEKYDNKTKYSYYKLIDKFGTHYITKVTLGGAVHSVTSIRQCHAALQGLSMDEVKTCLDVEASASMMGKMSVTTEYHHCQKVEDKTLNKKSFSSSFSDRVTDIIGGHTENAELLFSSDKDPAAYKDWLSSLPQHPEIISYSLNALHELLPEKKPTHEYLRNAIKDYILQRGLVKNCSKPCNAGIKTNPKEPCICSCHNNAGVNNDCCPARRGLAKVTVIAVRASGLWGDYFTQTDGYVKVLRNGKIVVGETPVIWNRNSPTWNWKFDLDINDLTQFTSVKLETWDRDNKWDDDLLGACQVQLKAGLQDNFCHLNHGLLYYKVQVTCIPSLTGPTCSKYVPSPMSSQLEKVYVSRHARPIPKDMLLEMGVLLDERSFNSSQSHEFQTVHKTKGFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M0B4</accession>
    <name>A0AAR2M0B4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RNA binding motif protein 4.1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017565773.1">
        <property type="nucleotide sequence ID" value="XM_017710284.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M0B4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062020.1">
        <property type="protein sequence ID" value="ENSPNAP00000082250.1"/>
        <property type="gene ID" value="ENSPNAG00000031673.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108434861"/>
    <dbReference type="CTD" id="573001"/>
    <dbReference type="GeneTree" id="ENSGT00940000164485"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12606">
        <property type="entry name" value="RRM1_RBM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12607">
        <property type="entry name" value="RRM2_RBM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000058">
        <property type="entry name" value="RNA-binding motif protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000085">
        <property type="entry name" value="RNA-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034897">
        <property type="entry name" value="RBM4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034898">
        <property type="entry name" value="RBM4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="78"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="12">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="319"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="45515" checksum="FC77540F40489547" modified="2024-11-27" version="1">MVKIFVGNLSPNTTTEELRSLFSQYGKISECDIVKNFGFVHMDDKAEAEEAIRNLHHYMLNGLAMNVEMSRGKPKASTKLHVGNISSSCTNQELRAKFEEYGPVVECDIVKDYAFVHMERVEDAMEAISGLDNTAFQGKLISVKLSTSRLRTAPGMGERTGCYRCGQEGHWSKECPLDQNGSYREGPGSEGYGPPRFGGGGGRGGRGFHRGGFNGEPAYSANYAPAHSFSRGAAYPSATGFGRGTGYDGAVSYGVPPGYSVGAVYGSEAAYGNSGAGYGSAVPGYPMRRAPYDERDPYGVVDYYEKYRARPYGTGYFEDRRAVPLPAPPPPTSSTIARDRLSSSNLDPYERRPIPPPPAPVSSYYTRDRSPIRRIPVEGEGYSYDRSRISPVSSLNRSSAYDMSRDPYGDRARYAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BLM1</accession>
    <name>A0A3B4BLM1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BLM1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000448"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013830.2">
        <property type="protein sequence ID" value="ENSPNAP00000000448.2"/>
        <property type="gene ID" value="ENSPNAG00000007516.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000289626"/>
    <dbReference type="OMA" id="TRCITRC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35151">
        <property type="entry name" value="ELONGATION FACTOR 1 BETA CENTRAL ACIDIC REGION EUKARYOTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35151:SF2">
        <property type="entry name" value="ELONGATION FACTOR 1 BETA CENTRAL ACIDIC REGION EUKARYOTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000448.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="156" mass="17113" checksum="C956275758F3FD0D" modified="2024-11-27" version="2">VYSKLCNTICIKPCNTVCIKPCIKVCNNPCNTVCNKPCNTVCIKPCNTVCIKPCIKVCNNPCNTVCNKPCKNSQVCNKLCDKACNTVFCIKPCKTVYSKFCNIICIKPCNTVCIKPCNKVCNKPCNTVCNKPCKTFVTHPVTVCNKPCKTASKKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNK1</accession>
    <name>A0A3B4BNK1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Fucosyltransferase</fullName>
            <ecNumber evidence="24">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">FUT9</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = a beta-D-galactosyl-(1-&gt;4)-[alpha-L-fucosyl-(1-&gt;3)]-N-acetyl-beta-D-glucosaminyl derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14257"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:137941"/>
            <dbReference type="EC" id="2.4.1.152"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:14258"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a neolactoside nLc4Cer + GDP-beta-L-fucose = a neolactoside III(3)-alpha-Fuc-nLc4Cer + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48376"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90376"/>
            <dbReference type="ChEBI" id="CHEBI:90379"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:48377"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>alpha-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + GDP-beta-L-fucose = a neolactoside IV(3)-alpha-Gal,III(3)-alpha-Fuc-nLc4Cer + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48380"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90380"/>
            <dbReference type="ChEBI" id="CHEBI:90381"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:48381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>alpha-N-glycoloylneuraminosyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + GDP-beta-L-fucose = alpha-N-glycoloylneuraminosyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-[alpha-L-fucosyl-(1-&gt;3)]-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48388"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90383"/>
            <dbReference type="ChEBI" id="CHEBI:90384"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:48389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56076"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:136545"/>
            <dbReference type="ChEBI" id="CHEBI:139509"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:56077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>an alpha-L-Fuc-(1-&gt;2)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc derivative + GDP-beta-L-fucose = an alpha-L-Fuc-(1-&gt;2)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77191"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:133510"/>
            <dbReference type="ChEBI" id="CHEBI:195560"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:77192"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68044"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:145343"/>
            <dbReference type="ChEBI" id="CHEBI:176900"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:68045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-D-Glc + GDP-beta-L-fucose = beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-D-Glc + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:77187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:60239"/>
            <dbReference type="ChEBI" id="CHEBI:61352"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:77188"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="24">Golgi apparatus</location>
            <location evidence="24">Golgi stack membrane</location>
            <topology evidence="24">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Golgi apparatus</location>
            <location evidence="21">trans-Golgi network membrane</location>
            <topology evidence="21">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 24">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="24"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNK1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000215"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014249.2">
        <property type="protein sequence ID" value="ENSPNAP00000000215.1"/>
        <property type="gene ID" value="ENSPNAG00000000139.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155095"/>
    <dbReference type="OMA" id="GYTALFM"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017083">
        <property type="term" value="F:4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000001">
        <property type="entry name" value="alpha-(1,3)-fucosyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF10">
        <property type="entry name" value="4-GALACTOSYL-N-ACETYLGLUCOSAMINIDE 3-ALPHA-L-FUCOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 24" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 24" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="26">
        <location>
            <begin position="98"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="25">
        <location>
            <begin position="219"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="395" mass="46725" checksum="F5BDD10570F5BAB6" modified="2018-12-05" version="1">MTSKLSTGRFQQIIVISVLLLCSAVIFYIYYKPMMSWLPCSKFVLPKQDVCTEECLHVLKVANQTQIITNCTAVQVKTPTSQSPVLEKEDTTNAHEPETVVLIWKWPFGLQFDLESCESNYGFKGCRLTLDRNQLDKAHAIMFHVRDIGGDVSYLQTLSRPPRQKWVWMNMESPDNSYRLPGADDLFNLTSNYRRDSDIWVPYGQLVDISENNQPFKIPEKDKLVCWIVSNWKDNLKRVRYFNELSQHIKVEAYGRHFGRYVNNEDYSQVVRSCKFYLSFENSVYIDYITEKLFNPMRLGTVPVVLGPARENYEEFVPRDSFIHVDDFQKPQELAEHLKLLDQNQEMYERYFDWRKDYVPKMNDFGQAQACHICDHVRRNKNYRVFKNVNKWYWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BP14</accession>
    <name>A0A3B4BP14_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SECISBP2L</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BP14"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000211"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014234.2">
        <property type="protein sequence ID" value="ENSPNAP00000000211.2"/>
        <property type="gene ID" value="ENSPNAG00000007362.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043356"/>
    <dbReference type="OMA" id="FPGEACG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035368">
        <property type="term" value="F:selenocysteine insertion sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000004">
        <property type="entry name" value="selenocysteine insertion sequence-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040051">
        <property type="entry name" value="SECISBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13284">
        <property type="entry name" value="GH01354P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13284:SF10">
        <property type="entry name" value="SELENOCYSTEINE INSERTION SEQUENCE-BINDING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="718"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="559"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="632"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="895"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1026"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="812"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="363"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="641"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="951"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="985"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000211.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1080" mass="117590" checksum="78FF20BF7C3EFCD8" modified="2024-11-27" version="2">MWYQKGFYSHIYGDMKLSAEVEPFIPQKKGVDSSLLSMSVCGEGGGGAEPTPIPSYLITCYPFVQENQPNSRQLPVYNGDLRWQQPGAAPGGPYLAYPILSSPQPPVSNDYTYYQIMPAPCPPVMGFYQPFPGAYAGPLQPSVVNPVTDGCERPPQQAQPFGMAAQRGRGMARAPVLPKQVAQPIRAKRPVMRSVAVQKEVGAAGPGGRSRTVLLVDAAQQTDFPGEACGGGGSSRCVSEGGLQWRGKPRRRRVSQPAESSSEQGASEADIDSDSGYCSPKHNHQGAASRSNQHTNTLGVEAGVMTAVSWVNAASQAGQKPWSDRASPFHRNKIHDQRSYPQEFPMGFVGRVGGVTERGPVGGQRRVQQGVQPTTHTTSTPSSTNTPSTSNNDHTPEPLYFQDEEEFPDLATGGAVQRNKQEQVQLKLPKPLLDNLPENSPINIVQTPIPITTSVPKRAKSQRKKALAAALATAQEYSEISMEQKKLQEALTKAAGKKSRTPVQLDLGDMLAALEKQQQAMKARQLNNTKPLSYTVGTTSVFHSKDAVSKGVIKTPYTPPLNMLDSTPPRIKRGKEREVPKVKRPTALKKVILKEREVKKAKLCVDQPAGAEEQPDDDLNFTDTLTQEMVSQEENGLSMPSDASLSPASQNSPYSITPVSQGSPASSGIGSPMATSAITKIHSRRFREYCNQVLNKEIDESVTLLLQELVRFQERVYQKEPAKAKAKRRLVMGLREVTKHMKLNKIRCVIISPNCEKIQAKGGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAEALFNRLVSLTEEARRAYKEMVSALEQEQAEEALKNVKKVSHHMGHSRNPSAASAISFCSVISEPISEVNEKEYETNWRTMVENSDGLEPLENESASKTSSGTKESERTSHTPPAEPASTQPAHSHPPGPEGQDRLEWASQQSTETGSLDGSCRGLLNSSITSTSSTLVPGMLEEEEEDDDDDEDYTPEPILVEVPPLSSMIETWVSTTLENLQLSGKSRHGSEEEEEDEDEEDVGGVSDDEDEVDLSDLAEPAVVDKQTAEAQNTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQB8</accession>
    <name>A0A3B4BQB8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HSF1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQB8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001803"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011522.2">
        <property type="protein sequence ID" value="ENSPNAP00000001803.2"/>
        <property type="gene ID" value="ENSPNAG00000001048.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163549"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010542">
        <property type="entry name" value="Vert_HSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF274">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06546">
        <property type="entry name" value="Vert_HS_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="10">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001803.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="533" mass="58875" checksum="4A859767B2FEAD79" modified="2024-11-27" version="2">MEFHTVGSGGLLVSSSNVPAFLTKLWTLVEDPETDPLICWSPHGNSFHVFDQGRFSKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPEKDDTEFQHLFFIRGQEHLLENIKRKVTAVSNIKHEDLKLSPDDMTKMISDVQQMKGKQESMDSKISTLKHENEALWREVASLRQKHAQQQKVVNKLIQFLITLARSNRVLGVKRKMPLMLNDSSTAHSMPKFSRQYSLEPSPPPGATFAGTGIFSSNSPVKTGPIISDITDTAQCSPTDKEDWIEDRTSPLVHIKEEPSSPTHCPEIEEVCPVEVEVGVGSSVPADTPLSPTTFINSILHESELVPHPTPSPTEHRCLSVACLDNSPQMSEVSRLFPSLSSSPHLRTHTGTEFSDHLDSIDSGLENLQSILNAQSINFDSSPLFDVNIQELLSPEPARETESGANSSKSGKQLVQYTAQPLVLPDPITTETTGDDLPILLELEGDSYFGQDPVEDPTVSLLSTDYQTAVPEDPKVGGVGLVKEPDFVLVLRSKAGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSR1</accession>
    <name>A0A3B4BSR1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the parathyroid hormone family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571217.1">
        <property type="nucleotide sequence ID" value="XM_017715728.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017571218.1">
        <property type="nucleotide sequence ID" value="XM_017715729.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BSR1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024511"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009936.2">
        <property type="protein sequence ID" value="ENSPNAP00000024511.1"/>
        <property type="gene ID" value="ENSPNAG00000009087.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438138"/>
    <dbReference type="CTD" id="554221"/>
    <dbReference type="GeneTree" id="ENSGT00390000004933"/>
    <dbReference type="OMA" id="EGTHLPQ"/>
    <dbReference type="OrthoDB" id="9892514at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003626">
        <property type="entry name" value="PTH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001415">
        <property type="entry name" value="PTH/PTH-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17223">
        <property type="entry name" value="PARATHYROID HORMONE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17223:SF0">
        <property type="entry name" value="PARATHYROID HORMONE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01279">
        <property type="entry name" value="Parathyroid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00087">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043960681" evidence="7">
        <location>
            <begin position="29"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="205" mass="22709" checksum="0A530D6B99AD45FA" modified="2018-12-05" version="1">MRMLCSRRLLQHWCLAVFLLCSPVPHYGRPIDALSNRMKRSVTHAQLMHDKGRTLQDFKRRLWLHELLQEVHTADIRDVQQRGGVSGGGGGAVNVGIPLGVGISVSPGTVHPKPAGGTKNLPISFNVDDEEGTNLPQETNKSQPYKDSAIKVGGKKKKKGRTGKRREGEKRKRRARSLSWALVDELGSGLHLEWRPLAGLRGVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BT65</accession>
    <name>A0A3B4BT65_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548126.1">
        <property type="nucleotide sequence ID" value="XM_017692637.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BT65"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002837"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009759.2">
        <property type="protein sequence ID" value="ENSPNAP00000002837.1"/>
        <property type="gene ID" value="ENSPNAG00000009175.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424546"/>
    <dbReference type="CTD" id="321378"/>
    <dbReference type="GeneTree" id="ENSGT00950000182887"/>
    <dbReference type="OMA" id="TSRHFLM"/>
    <dbReference type="OrthoDB" id="298939at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031701">
        <property type="term" value="F:angiotensin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002031">
        <property type="term" value="P:G protein-coupled receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000003">
        <property type="entry name" value="beta-arrestin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.840:FF:000001">
        <property type="entry name" value="beta-arrestin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000698">
        <property type="entry name" value="Arrestin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017864">
        <property type="entry name" value="Arrestin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014753">
        <property type="entry name" value="Arrestin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792">
        <property type="entry name" value="ARRESTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792:SF20">
        <property type="entry name" value="BETA-ARRESTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00309">
        <property type="entry name" value="ARRESTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00295">
        <property type="entry name" value="ARRESTINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="5">
        <location>
            <begin position="194"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="415" mass="46707" checksum="B6886E6987FAF42C" modified="2018-12-05" version="1">MGDKAGTRVFKKSSPNCKITVYLGKRDFVDHLDQVDPVDGVILVDPEYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLYISTFQAFPPIPEERKPNSRLQERLLKKLGQHAHPFHFTIPQNLPCSVTLQPGPEDTGKACGVDYEIRAFCARSVEEKIHKRNSVRLVIRKVQYAPEKPGPQPMVETTRSFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSTKTVKRVKISVRQYADICLFSTAQYKCPVAQLEADDQVSSSSTFCKVYTLTPTLNNNREKRGLALDGKLKHEDTNLASSTIVKDGCNKEVLGVLVSYRVKVKLVVSRGGLLSSLLERDVSVELPFVLMHPKPIDLSSSRPASAVPEADPPIDTNLIEFDTNSFTQDDDLVFEDFARLRLKGTTDDKEEDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BU20</accession>
    <name>A0A3B4BU20_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plexin-B1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for SEMA4D. Plays a role in GABAergic synapse development. Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037397230.1">
        <property type="nucleotide sequence ID" value="XM_037541333.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000002051"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011090.2">
        <property type="protein sequence ID" value="ENSPNAP00000002051.1"/>
        <property type="gene ID" value="ENSPNAG00000001111.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429288"/>
    <dbReference type="CTD" id="561012"/>
    <dbReference type="GeneTree" id="ENSGT01150000286928"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12793">
        <property type="entry name" value="RasGAP_plexin_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000010">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000012">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000126">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000705">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000120">
        <property type="entry name" value="Plexin b1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000203">
        <property type="entry name" value="Plexin B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057533">
        <property type="entry name" value="PSI_Plexin-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF59">
        <property type="entry name" value="PLEXIN-B1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24317">
        <property type="entry name" value="PSI_Plexin-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24479">
        <property type="entry name" value="PSI_PlexinA-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18020">
        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017411595" description="Plexin-B1" evidence="19">
        <location>
            <begin position="18"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1502"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="20">
        <location>
            <begin position="8"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="842"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1283"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="854"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1289"/>
            <end position="1298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2145" mass="236286" checksum="7746EEC0624FC8DF" modified="2018-12-05" version="1">MALIFTLLLPLLLGVLGTPLVCSHYPRFSSNDTAFQHLALHPDPAVGTVYVGARDHLYQLASLDGLRLEVEERTGPVRDSKECLPPVTLDNCPQARLVSNHNKLLLVDPYSLQLITCGSAHQGTCQKRSLASVSNVLFSAERPVDTQYVAANDPAVSTVGLVVRPRDGAPSLLYVGRGYTSSHPPISTRQLSAEPIFSYEETAKLAVAGRLSEYDHNFVATFTHHNHVYFLFYRRNIKGASREYITYVARVCLDDQAYYSYVEVPLACRSGSTGRNYNLLQGAQVGGNEAGEGQVLMAVFSTALSAASKPSEESALCVYALDELDGHIDATRDLCYTQDGQKEGRKVAYIEYDVKSSCANLPTNTLKAYPCGSDHTPSPMASWEPVEAEAVLDSPARLTAVAVSVREGHSIAFLGDSKGNLHKVFLGRSGEVKNYSTLSIQPDSPINRDLLLDSSQKHLYIMTSNTVEKRPVAECERHKDCQSCLAARDPYCGWCVLEGKCGVRSDCRRGSGQGHWLWSFDEEQKCLRVESLSQHNISIGEQQNVALEVPGLPSLSDGEEYSCFFQETQSTATVNATTVTCSTPPAHRIPTVPHGQDSLTVTLSLRFLNVTVAVQEFTFYDCSLVQQLSGTMPCIGCVSSRWRCNWCIHQHVCTHQAMCDRGVIIYNQHFKLPASSPTTTSDFTKFTTSMPVRPTTAPHKESMPTTAVAVSALPVAPPSTPEPAVIPSTTMGTTPPLRNLPTTFQHTMPSSTQQAPTTVAGADTLLKDSIAGRKVAHVGLLSTPAPGGHGEDTADVSHKESAVDSVTVPASTALSGDAGAMGPLGEALPPSSTAVVTLWSEENDDGEASLETGPPVSSASAPSGDGEEEPISTEFSHAQDVNYQSDPAEFFLPGDEDSLVSGGSFACPCVETVQGSSLLPVNIERKVTLVGRNLHLYQDEELDYECVLSIEGRSVVVAAFVEMDDSQSSLFYITCQLHQYSYSAAVEEYNAAITVRRRGSFQMDSPQGLHVRLYNCSVGRSDCSRCRTAQQQYGCVWCEGAHSSCVFNSSCTDHIQHTCPAPHIHLIEPLSGPVEGGTVITISGSNLGQRAEDIQHSVTVAGAPCRVIPSRYEVSSSIVCETTASGGERRGQVSVEVKEGGTGQSVQHFHYQDPVLLGVSPLKGPMSGGTSLTIMGRGLRTGQLSEISVLIGGVPCLISSELEDEQIKCLSGGCNRTGEHGVTVQFGSAERHLQTSSYHYTPDPNITSAAPCKSFLSGGRVIRVSGQNLDVVQEPRIRVTLSPLEPHFSGRKRRKRRSDGHSRREDRLWPLKRQRRIVPEPGCPEDSLCDVKQIEEHCTVNSSTLLLCPTPELGPEARHAVINVHFLLDNLSFDFKAVSGSPFSYELNPILHPLNRGDPSKPYRHKPGSVISVEGENLDLAIFKEEVVALIGDGVCAVKTLTRNHLYCEPPTQQPSVSASKKREGTDALPEFTVRMGNLNFSLGRVQYDSQGQAMFPLEAQVGVGVGAFIVALIVLVIVLIYRRKSKQALRDYKKVQIQLENLETSVRDCCKKEFTDLMTEMMDMSSDLVGSGIPFLDYRTYAERIFFPGHRESPLRRDLDVPACRRVTVEQGLVQLSNLLNSKLFLTKFIHTLEGQRTFSPRDRAYVASLLTVALHGKLEYFTDILKTLLSHLVEQYTTKNPKLMLRRTESVVEKLLTNWMSICLYSFLRDSAGESFYMLFRAIKHQVDKGPVDAVTGKAKYTLNDNRLLREDVEYRTLTLNVLVTAGGASESQTVPTKVLDCDTITQVKEKILEQTWKGTSYSQRPSTDSVHLEWRAGMAGHLILSDEDLTSVVQGSWKRLNTLQHYKVPDGATVTLVARQSKQVHHDSHDYVPGEKTPMLEDGDEGGVRQWHLVKASEEPELSKHRRGSLRERERERAKAIPEIYLTRLLSMKGTLQKFVDDLFTVILSTSRPVPLAVKYFFDLLDDQAAQHNITDPETIHIWKTNSLPLRFWINILKNPQFIFDVQASDNVDAVLSVIAQTFMDSCTIADHKLGRDSPINKLLYARDIPRYKQMVERYYADIRQTISASDQEMNSALAELSRNYSSEVNHLVALHELYKYINKYYDQIITALEEDSTAQKMQLGYRLQQIAAAVENKVTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BV90</accession>
    <name>A0A3B4BV90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH La-type RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LARP1B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the LARP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572227.1">
        <property type="nucleotide sequence ID" value="XM_017716738.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BV90"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002410"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010478.2">
        <property type="protein sequence ID" value="ENSPNAP00000002410.2"/>
        <property type="gene ID" value="ENSPNAG00000008823.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438733"/>
    <dbReference type="CTD" id="55132"/>
    <dbReference type="GeneTree" id="ENSGT00940000154718"/>
    <dbReference type="OMA" id="TGRHNAF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08034">
        <property type="entry name" value="LARP_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000131">
        <property type="entry name" value="la-related protein 1B isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006607">
        <property type="entry name" value="DM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF50">
        <property type="entry name" value="LA-RELATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21071">
        <property type="entry name" value="LARP1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00684">
        <property type="entry name" value="DM15"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="5">
        <location>
            <begin position="291"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="411"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="645"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="934"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="411"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="448"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="684"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="750"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="989"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1015"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1049"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1080"/>
            <end position="1099"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002410.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1099" mass="123190" checksum="67EF548FFB52BA20" modified="2024-11-27" version="2">MSTVDSAVRDGDQNRDNSKDDPGAKVELPQEKPAKVGGEEQNNTAEKENEPSTTSTDTKEGENGKTKVLEAPPPKVNPWTRKSSSTCPPSTQTDSQNPAKVVRASKPRSRKSSKSSDFSDITNWPTPGEIANKEQQQNVINTQGRKPTVGRRERRRDRGGEKREKESGSDGSDSKENREAQQEPMTHLPTEQGKETGKEMQNSASRRRGGWRRERDFFNDASSIRSEGSSVRDSLRGRGKGRGRGRGRGRGRYDYSHSYRDSYVDGSSVPQEFGTNMMYFYDDGNRIQMYTIEESLLKEYIKRQIEYYFSLHNLERDFFLRRKMDAKGFLPISLIAGFRRVQALTTDINLIMEAVKNSDVVELVDEKIRRKEDPEHWPIPGPPVDSPRTDFTQLIHCPEFVPGQTFTCLNAGSTSPTNENTVESTPSTNGQLNGSSDTEAKPNSSKSSEAESKSVSKAEERTPAGLSNSVPDAETPRGLSSSLPDYENQWIQVEKRHRNTSGKNKERSVLVQSGPDLQPEADQEELDFLFDEEMERLAAPRKNTFTDWSDDDSDGELDDRDVNKILIVTQTPPHLRKHPGGDRTGNHVSRAKITAELAKAINDGLYYYEQDLWTEEDKQLDSTSMKELENFKKISMISQDEFDTLTPKVPIDPKQEVPPPPPCQDESSVAHSVPTDVPEPSSAPRTPRTPRTPGRQDPTKTPRFYPVIKDSGNIDGQTPRKRKTRHSSNPPLESHVGWVMDSREHRPRTSSISSSNASPSEGAPLQSGSYGCTPQSLPKFWHPSHELLQENGFTQQVYHKYRRRCLNERKRLGVGQSQEMNTLFRFWSFFLRDNFNRKMYEEFKQCALEDAKENYRYGLECLFRFYSYGLEKRFRPEIFKEFQEETLNDYEKGQLYGLEKFWAFLKYSKVKGQVIDPKLQEHLCKFKSIDDFRVEPPMGDEGGRRRRHSSSGGDESRRKRHNSSSSSSSKQAPPTKPLNHPAAAAAAKTDSNSRSASPANTKTTSANQGPVKAASGSQSGGVANSKTTSASQPAAAGPAKTPSANQKVATSSAKSTAANEPATTTRVAPKENVKKIAQSEMRRGEQAAIKTKDAHPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWF7</accession>
    <name>A0A3B4BWF7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Baculoviral IAP repeat containing 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">BIRC5</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWF7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002840"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009743.2">
        <property type="protein sequence ID" value="ENSPNAP00000002840.1"/>
        <property type="gene ID" value="ENSPNAG00000009182.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047537"/>
    <dbReference type="OMA" id="HAPQCEF"/>
    <dbReference type="OrthoDB" id="2196114at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000009">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
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        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF3">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="141" mass="16330" checksum="3E985E0A597B2E95" modified="2018-12-05" version="1">MELMNEDHLKMYLCSNRLATYSGWPFDEGCACTAETMAKAGFIHTPTDNSPDVVQCFFCYKELEGWEPEDDPVKEHVSHSPKCHFILLKQAVEELTVEGFLKLQKERQKFLIMKNCNQAIEKFEESAKSRRRDITKMAMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXE7</accession>
    <name>A0A3B4BXE7_PYGNA</name>
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        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_017690858.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="BZIP_BASIC"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="107"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="198" mass="22398" checksum="55BB9CC19BB1207A" modified="2018-12-05" version="1">MQRFPLFKIYSRPPADLKKAHPVSSAPKAAVVKGVCDMMPGQIPDPSVTAGSLPSLGPLAGISATTLTDQLKYADLCSLGAMLSPLHLLGKLGKRHLPIKAEKDEEEERRKRRREKNKVAAARCRNKKKERTEYLQRESERLEMMNSELKAQIEELKHERQQLILMLNRHRPTCIVRTDSVKTPESETNPLLEQLEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYC8</accession>
    <name>A0A3B4BYC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polyhomeotic-like protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYC8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003565"/>
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        <property type="gene ID" value="ENSPNAG00000009988.2"/>
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    <dbReference type="OMA" id="EGCAGRD"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:histone binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="SAM_Ph1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polyhomeotic-like protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PcG_chromatin_remod_factors"/>
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        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012313">
        <property type="entry name" value="Znf_FCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038603">
        <property type="entry name" value="Znf_FCS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF86">
        <property type="entry name" value="POLYHOMEOTIC-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16616">
        <property type="entry name" value="PHC2_SAM_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21319">
        <property type="entry name" value="zf-FCS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_FCS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FCS-type" evidence="11">
        <location>
            <begin position="649"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="10">
        <location>
            <begin position="803"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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    <feature type="region of interest" description="Disordered" evidence="9">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="41"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="544"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="703"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="726"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003565.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="867" mass="92262" checksum="548F0BC23336C120" modified="2024-11-27" version="2">MFTHPDSKSPFLISLRRVCQRGSRGSKVRRRGGTMEGEQGTSSSSTNTNTPSATTSTSSTTNPPNTPSSSSSSIASSASSTSSSRQTVPQISVYSGIPDRQTVQVIQQALHRQPSTAAQYLQQMYAAQQQHLMLQTAALQQQHLSTAQLQSLAAVQQASIAAGRQNSTQNGTSSTQSGSSQTTINLTTSPATAQLISRAQSVNSAPSGISQQAVLLGNASSPTLTASQAQMYLRAQMAQQSNLVHVARSLGRAVPLSPQLILAPTATVTALQSDTSTNTNTSSASAQVQNLALRSHQGTSSSSQSTGLQALSLKQTPVSIQPTPLLKTTSSGTTTGKGVLPGASGDAGKKQESGGETEAKAVNMSRSVTSSHPLIAPAYTQIQPQALIKQQPQQFVIQSQAPPTPRMQTLLQTASIQPHAQTASLGIQSSTQTSSGPIPVLPKPTIHNQGTAPSQHATIFHSTVSQHGAHSGLGQPKAQPVQLTAINLQIQPAHNQASRIAQDNKEKPTSLVMREICPPLVTQVPTSIPSQETNPPEPPKADTGTATIQTDGSAETGTVSAHMTSGAESARPASADSRPPQAVVKPHVLTHVIEGFVIQEGAEPFPVERNPVQTNSHKKAEQQLPSHPEKTPLSNFYLFFTDAGGRDEEEPDEPKLTCEFCGWVDFAYKFKGSKRFCSMVCAKRYNVGCSKRIGLFHPDRSKTANRWKRRSHTRGRRSTEAKKQKLSVSQQSQGESVSSPLPSQPSQGESSPCSDISSYEEPPSPLSAASSGPVGPPRGVTTSDPEDLPLLSQHFLPCDPTKWNVQEVFEFIRSLPGCQEIADEFRSQEIDGQAMLLLKEDHLMSAMNIKLGPALKIFARINMLKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C219</accession>
    <name>A0A3B4C219_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C219"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004622.2">
        <property type="protein sequence ID" value="ENSPNAP00000005823.2"/>
        <property type="gene ID" value="ENSPNAG00000011945.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01080000257344"/>
    <dbReference type="OMA" id="SSAVYTW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001495">
        <property type="entry name" value="Si:dkey-234i14.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="19"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005823.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="126" mass="13365" checksum="B085F2FEF3E14FFA" modified="2024-11-27" version="2">MEVFGESISIEAPLCMISPRFSALLVTVTQTPAVKTVSPGDSVTISCRTSSAVYTDSAGHCLAWYQQKPGEAPKLLIYAATSRQSGVPARFSGSGSGSDFTLTISNSEDAGDYYCQQGYDTPFTQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3G2</accession>
    <name>A0A3B4C3G2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Actin binding LIM protein family, member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ABLIM2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3G2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005134"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005816.2">
        <property type="protein sequence ID" value="ENSPNAP00000005134.2"/>
        <property type="gene ID" value="ENSPNAG00000011468.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182850"/>
    <dbReference type="OMA" id="NTNCAYL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09327">
        <property type="entry name" value="LIM1_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09328">
        <property type="entry name" value="LIM2_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09329">
        <property type="entry name" value="LIM3_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09330">
        <property type="entry name" value="LIM4_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000003">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000004">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000007">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000053">
        <property type="entry name" value="Actin-binding LIM protein family, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032402">
        <property type="entry name" value="AbLIM_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF6">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16182">
        <property type="entry name" value="AbLIM_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="87"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="155"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="HP" evidence="11">
        <location>
            <begin position="468"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="348"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="381"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005134.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="60205" checksum="28AAE9385A56E6F9" modified="2024-11-27" version="2">VPAVTFQQQAVHGPLEQSKGPPHSGPIPCQNCGKACKGEVLRVQNKHFHIKCFVCKVCGCDLAQGGFFVRQGEYICTLDYQRLYGTRCFSCEQFIEGEVVSALGKTYHPRCFVCAMCKQPFPAGDRVTFNGKECICQKCTQPLPANSPAPVQAVHNCCGCGKEFKNEQSLVALDKHWHLGCFKCKVCNKVLNAEYISKDGIPYCEMDYHAMYGIQCENCKKYITGKVLEAGDKHYHPTCARCVRCEQMFGEGEEMYLQGSSIWHPPCRQAARMEEKSKVTRTSSESITSAPASSASGSPSRVIYAKLGDEFLDYKDLAALPKIKAIYNIDRPDMLSYSPYVSYPAEERTYAEGDGEKSPRQRRPSSPSSNSSLGGYGRYTPSRSPQTYSRPGSESGRSTPSLSTYSDGKSPSSTYVAAPKHFHIPDTKAKDNIYRKPPIYKQHGTVRSTRTSWTILKSEIDGQSPDIDPRTSTHSLPTDMSQPSEVRVKLPRDVDRTRLERHLSPEEFFQVFGMTIEQFDRLALWKRNDLKKKARLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4K2</accession>
    <name>A0A3B4C4K2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017576441.1">
        <property type="nucleotide sequence ID" value="XM_017720952.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017576443.1">
        <property type="nucleotide sequence ID" value="XM_017720954.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4K2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005791"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004696.2">
        <property type="protein sequence ID" value="ENSPNAP00000005791.1"/>
        <property type="gene ID" value="ENSPNAG00000002002.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441433"/>
    <dbReference type="GeneTree" id="ENSGT00940000166341"/>
    <dbReference type="OrthoDB" id="5232919at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF11">
        <property type="entry name" value="HISTONE DEACETYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="12">
        <location>
            <begin position="55"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="503"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="255"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="581"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="615" mass="68770" checksum="FF8059ADB3EC5534" modified="2018-12-05" version="1">MLQTIYESDTRIPAEWLRTILDTHSISSTVIDSGSEVLSPVSRSEVSMPEWERDSDEWERQLQRELLLIQRQQQIQKQLLISEFQKQHQALLRQHQAQLQEHIKLQQALLTLRQQQEALAEEHRPEEEEEEKEEVQGEELERNKKEQQHLNQRKKERSKESAMASSEVRQKLHDFLLSKSAKDGSPSGHPLNQHSKLWYITSQHMSLEQNSPPLGEMPPKFKSPLPSGLDTREDFPLRKTASEPNLKVRSRLKQKVSERRNGPMQRRRDGSVLVTPPKKRSLEPSDSSANDSSPSSGPSSPIGLCSNENGASYRPSAAQLEKYLPQSGIMKADGSMALLNLYTSPSLPNLTLGLHSGASPISSTTSLKGHAGEVVAGQPVSGVPAVCREAKVNSSHQALLQHLLLKEQNRQQKMLSPGSVSVLPQSPLVMKERSGGGARSRLPRHRPLNRTQSAPLPQSMLAQLVLQQQHHNFVEKQKQYHQQVHINQMLSQSIEQLRNPTAHLEEDEEGVSGAEAKGQELTLPPAGVIRTHSSSSSRLENGTQTEAPPAQVGVIRVKEEPLESESRMEEERRGDGGAVRRRNEGMKASEEQLKDAALLQVKTEQCAESMTETDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C526</accession>
    <name>A0A3B4C526_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017554250.1">
        <property type="nucleotide sequence ID" value="XM_017698761.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017554251.1">
        <property type="nucleotide sequence ID" value="XM_017698762.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C526"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006175"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004021.2">
        <property type="protein sequence ID" value="ENSPNAP00000006175.1"/>
        <property type="gene ID" value="ENSPNAG00000002159.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428051"/>
    <dbReference type="GeneTree" id="ENSGT00940000166053"/>
    <dbReference type="OMA" id="LDQGQCE"/>
    <dbReference type="OrthoDB" id="426001at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000028">
        <property type="entry name" value="Dual specificity phosphatase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF314">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="737"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="800"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="205"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="354"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="605"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="898" mass="97401" checksum="7992C253E60894B9" modified="2018-12-05" version="1">MPPLPLDERIVVIQRPKSLALRVASPPLTSQSSSQRLTTHNEPPPRSPQPQPPPPRPYPPSNSLSLECRHRHLSRSQRAKGERGATTEDSTRVPSHCHSNGTVTLGPRPHKHTHTIDIKVSVDKGGTHLDKGHSSSLTRGGSVREGRSKRVSLHVDPGQGDRKPRGKSSEPAERHLIQTKTQHGSHHQNHLAVSPGGVLEFIPAQRQQSKPRRAREEDLSLKSHSATANYRDVPSLGNKENSKLGPVQQPPNPHSLPRHHSSAPSPHASILNTHYPAAPPSVPFHAPSSFQHISQTRPSRTREQRPQILHGLPLSPTSSRGGFPSPDHTCTIDFSRPFNCGCWKLVRCRGGRGHSAGCQGGTGSPSSFSQAQGATSNKRGDATMGLKTLGGCLSTASTTNTSSSNSTVSDCRTGLLKPLRCASCSGEGRGFESPAAFRKKLVGGCLPCAPLSSSAPLRALQSCVSGCNPKASQTGSSTCSYCSSDPIVVTFNPHRAKPPNMGHSIGAHTMGGYQPDDDDYSVRTIWPEELAKKMTRSKTQPNHHNAAMGVGTARSCIGHDQSGSNGTSPVILDCRNLLEFTRSQLTDHAGRRRLQQGKMAVLDFMGSGSSRDQGRDSLKRLWNKGGDARMGDDDGLDDDMLPRSPSPRSPPSESPPSFSPPPSAPGTLIKPKPRGREREGGHSLPSAQSLHLVLNSLNREQEDEENGRVHLSLPLSSSLPASLSDESVMTPDVENAVVSPILPFLFLGNERDAQDLDLLLRLNIGYVVNVTTHLPLYHVDTGLVRYKRIPATDNSKQNLRQYFEEVFEFIEEAHQSGRGVLVHCQAGVSRSATIIIAYLMKHTLMTMTDAYKYVRGRRPVVSPNLNFMGQLLEFERDLNSGVTPRILIPKLSGLETQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9F1</accession>
    <name>A0A3B4C9F1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hypoxia-inducible factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037394157.1">
        <property type="nucleotide sequence ID" value="XM_037538260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9F1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008258"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000455.2">
        <property type="protein sequence ID" value="ENSPNAP00000008258.2"/>
        <property type="gene ID" value="ENSPNAG00000013861.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440802"/>
    <dbReference type="CTD" id="797150"/>
    <dbReference type="GeneTree" id="ENSGT00940000156774"/>
    <dbReference type="OMA" id="LNCEPIP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905517">
        <property type="term" value="P:macrophage migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045765">
        <property type="term" value="P:regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000005">
        <property type="entry name" value="Hypoxia-inducible factor 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000015">
        <property type="entry name" value="Hypoxia-inducible factor 1-alpha isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014887">
        <property type="entry name" value="HIF-1_CTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021537">
        <property type="entry name" value="HIF_alpha-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF7">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11413">
        <property type="entry name" value="HIF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08778">
        <property type="entry name" value="HIF-1a_CTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="15">
        <location>
            <begin position="16"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="93"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="238"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="347"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="547"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="548"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008258.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="731" mass="80812" checksum="874A115CD65697E4" modified="2024-11-27" version="2">MTSGATPEKKRVSSERRKERSRDAARCRRGKETEVFYQLARELPLPHSVTSNLDKASVIRLTLSYLRLRSLLHTGALSEGCELDSRWSVCCQKALDGFLLVLSQDGDVVYVSEYVSKCLGLPQIDLLGQSVFDFTHPCDHEEIRETLVHRTGPSKKGKEEHQSERRFLLRMKCTLTSRGRTVNIKSATWKVLHCSGHVQEGTGAHASYLVLNCEPIPHPANIESPLDSRTFLSRHTLDMRFTYCDDRVTELLGYDPKDLLQRSVYEYYHALDSDSLTKTHHSLFVKGQACTGQYRMLVKTGGFVWVETQATVIYNSKNSQPQCVVCVNYVLSGVEEPELVLSVHQSNSALVKQEREDEEEERESERGQQECSRKMRSKEEASSEREEQTAVLFDDLKGEPEALTQLHFSSTDTELGVLKDVPLYSDVMLPSTSVLLPLSPLSPPTEDPCATLSHSDSTATQLQLEDFSFPSSNTPSAVGGITCSSTQPISSLTSDLSNQCKVDVAEMFSMETEPKTLLDTQAVEDLDLEMLAPYISMDDDYQLHVHSPAQSGRVSPVPTSKSAQKDSPNRTQTLTCSTGTNIKPSEQLTAAQPQQSSNRACQPDLHLSSATQPHQKPLKRKLETISLSQAIGLGSVLQVVSDLPGAGKRVRKSEVVGLEAVSQPTVPESVTILLLPSDVLNQLLAGPSEGRTLSVPLPQLTRYDCEVNAPIAGRQNLLQGEELLFALDQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDU8</accession>
    <name>A0A3B4CDU8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytosolic fatty-acid binding proteins domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CDU8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009051"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035162.2">
        <property type="protein sequence ID" value="ENSPNAP00000009051.2"/>
        <property type="gene ID" value="ENSPNAG00000036907.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000064423.1">
        <property type="protein sequence ID" value="ENSPNAP00000068757.1"/>
        <property type="gene ID" value="ENSPNAG00000036907.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000079328.1">
        <property type="protein sequence ID" value="ENSPNAP00000064905.1"/>
        <property type="gene ID" value="ENSPNAG00000036907.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156713"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="3">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009051.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="132" mass="15197" checksum="07AC72C40EACD048" modified="2024-11-27" version="2">MEAFFGNWKLVSSENFEEYMRTIGWAEEVVQIGNIVTPVLSIYQDGEKLVMKLKTSVFSSEKSFVLGEEFDENFAGQKYRSVINLDDGKLVQTQKWDGKMTVQTRKIQDGKMILTLSYKDTVAVRTYEKMAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEQ5</accession>
    <name>A0A3B4CEQ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing G-protein coupled receptor 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LGR4</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017559005.1">
        <property type="nucleotide sequence ID" value="XM_017703516.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEQ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009983"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016500.2">
        <property type="protein sequence ID" value="ENSPNAP00000009983.1"/>
        <property type="gene ID" value="ENSPNAG00000015482.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430803"/>
    <dbReference type="CTD" id="55366"/>
    <dbReference type="GeneTree" id="ENSGT00940000157925"/>
    <dbReference type="OMA" id="PPGNCSM"/>
    <dbReference type="OrthoDB" id="1883493at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021888">
        <property type="term" value="P:hypothalamus gonadotrophin-releasing hormone neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:002032">
        <property type="entry name" value="Leucine rich repeat containing G protein-coupled receptor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF67">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017248984" description="Leucine-rich repeat-containing G-protein coupled receptor 4" evidence="20">
        <location>
            <begin position="26"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="527"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="564"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="660"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="700"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="746"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="788"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="21">
        <location>
            <begin position="543"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="974" mass="104492" checksum="F6221E21EC194F13" modified="2018-12-05" version="1">MPSLVAIRLLVLVLGLCSLRVAGQGRSTPATCSPLCRCDEDGGADCSGKGLSTVPAGLSAFTYYLDLSMNNITELPANVFKNLPYLEELRLAGNDLVFIHPEALSGLYQLKVLMLQNNQLKTVPSAALKNLHALQSLRLDANHITVVPGDSFEGLQQLRHLWLDDNSLTEVPVGPLQHQGNLQALTLALNRITHIPDNAFANLSSLVVLHLHNNRIQEIGKNCFNGLDNLETLDLNFNNLKSFPEAIQTLPKLKELGFHSNNIAIIPEGAFHKNPLLRTVHLYDNPLSFVGRSAFQNLSDLHSLMLRGATLVQDFPSLTGTGNLESLTLTGTKIRAIPEDLCEDLSVLRTLDLSYNEIEELPSFQGCVSLQDVTVQHNRVQQIDRDTFQGLTSLRVLDLSRNQIKFIHRDAFLSLSALTNLDLSMNFLASVPTAGLNALNQLKLVGNPDMKNSLTAKSLPKLRSISVPYAYQCCAFMTCDSTHSSPESEEKRKDLGGDEEMERVPLVMHCSPSPGAFKPCAHLLGSWMIRLTVWFICLVALLFNCLVLAASFSPRASPLSPARLLVALLASSNLLTGLYVAALTLLDVVTWGSFATYGVRWETGAGCQALGVLAVFSSEWAVLLLPLAAVERSLAVRALMGKAGILRGGGGERREGRKRFGLAAALLGLLAVVAAFLSLYHGSAGGSPLCLPFSEGPGPGLGVTVALVLLNTLAYLLSAVVYTRLYCSLGRARLADPEQAGSVRHVAWLIFTNCIFFCPVAAFSFAPLLAGAGNAAGGPEMAKSVTLIFFPLSACLNPVLYVCFNPAFRHDWLRLRGRGMGGACDGRNGKAVAVGTGSGGSSRAGESGGSRQETGELGYECGMYTQLCEHCEAALLARTSSSSASSSSSSSACRHLVKSHSCPALAGGEAPCPRAEGCWADSGTLWAQSEYADEGDSFVSDSSEQVQACGRACFCQSRGLPLVHYSYNIPRVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGX1</accession>
    <name>A0A3B4CGX1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGX1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016140.2">
        <property type="protein sequence ID" value="ENSPNAP00000009714.2"/>
        <property type="gene ID" value="ENSPNAG00000015188.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287067"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009714.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="30" mass="3092" checksum="AEE136EDDECA8A07" modified="2024-11-27" version="2">LLTTPSMSVMSGGAIKDCTMNLCLLFSLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CHW9</accession>
    <name>A0A3B4CHW9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CHW9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010945"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017737.2">
        <property type="protein sequence ID" value="ENSPNAP00000010945.1"/>
        <property type="gene ID" value="ENSPNAG00000016533.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286914"/>
    <dbReference type="OMA" id="KHGGGVM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13384">
        <property type="entry name" value="HTH_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30589" checksum="3148DA039DF455D3" modified="2018-12-05" version="1">MARKKQLSKEKRVAIITLRNEGQSVQKIGKTLKVSPSAVAKTIKRYKETGSHEDHPRKGRPRVTSAAEDKFIRVTSLRNCRLTAAQGYQVNATQSSSSRHISRTTVKRSLHGKIAARKPLLRTSNKQKRLVWAKEHKEWTLDQWKSVLWSDESKFEIFGSNHHVFVRRRKGERVDSTCLVPTVKHGGGGVMVWGCFAGDTVGDLFKIEGILNQHGYHSILQRHAIPSGWRLVGPSFIFQQDNDPKHTSRLCKGYLTKKESDGVLRQMTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CI88</accession>
    <name>A0A3B4CI88_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CI88"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010536"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017224.2">
        <property type="protein sequence ID" value="ENSPNAP00000010536.2"/>
        <property type="gene ID" value="ENSPNAG00000016139.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157073"/>
    <dbReference type="OMA" id="HKEWPEI"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF187">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX CONTAINING 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="508"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010536.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="559" mass="61870" checksum="06EE003BF414E862" modified="2024-11-27" version="2">MDADDVDEDEQLLNYAIQMSLEESYSNAFPSTITLVFFIQGDLFTLQEMSDYPAAFTEVDAKGWYPLHRAAVQQSVQVLEMVLYASYRLSLDEETEDGETPLTLAIQAGLVEIVRTLLAHGASPHRTNRKNESPLLLAVRTDEYDIVLALLTRGTQVNQVCLKKWTAMHEAAKVGSTDILKLLLEHGGQVSETDQSGVTPMGIAAEYAQAEVLDILIHNGADVNAQAPNGDSVLYDAAGSGNPDCVDLLLQNGANPNIPNLSSQLPIHRAAYEGHFLVLKILIPVTTRRALRLSGQSPVHTAADGGHVQCLELLLEKGFDVNALLDQHISENYGDMRRSPLFFTVSNGDITCAELLLNAGAKPDLDPLSCLVVAVRAGRYEIVKLLLANQADVNFYFTAVSDTVFPTALQYCLRDEMMMRMLLNCGYDAGKCFSCFHDSWGNNSHHSDNTTEKVPFCDFISVSWLAQCAGRAVRVLLDYVCYVPICSKLKMVLEKHKEWPEICDILGNPRTLKHMCRLEIRRRMTPKGLGDPAIFSSAPFPPGLQGFLMYKEYDLYGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJ25</accession>
    <name>A0A3B4CJ25_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G protein pathway suppressor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GPS2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJ25"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018320.2">
        <property type="protein sequence ID" value="ENSPNAP00000011370.2"/>
        <property type="gene ID" value="ENSPNAG00000017079.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004049"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026094">
        <property type="entry name" value="GPS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22654">
        <property type="entry name" value="G PROTEIN PATHWAY SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22654:SF2">
        <property type="entry name" value="G PROTEIN PATHWAY SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15991">
        <property type="entry name" value="G_path_suppress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011370.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="35518" checksum="1444C5D9B51351C5" modified="2024-11-27" version="2">MPALLERPKLSNAMARALHKHIMRERERKRQEEEEVDKMMEQKMKEEEERKRTKEMEERMSLEETKEQVQKMGEKLQGLQEEKHQLFLQLKKVLHEEEKRRRKEQRWIIKLVLFLLPVSHSRPGGLLADRGKQLFQTPGIPGRHFPGQPSFTPGSSEHGQFSGSPAVHDSYAVSQAQHPSTYGPGASVPISFSSSSQIRGASAFQTMPYLPHQPQGYAVHSHFTSPPGFIPSASIPLQKQLEHANQQSGFTDSGTLRPMHPQALHVSATGLLSTPSMAVQIPTAKASFQSSPQSAPRHAFLSHPQAGQRFYHHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CM71</accession>
    <name>A0A3B4CM71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing CTD phosphatase 1</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nuclear proteasome inhibitor UBLCP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">UBLCP1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017560649.1">
        <property type="nucleotide sequence ID" value="XM_017705160.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017560650.1">
        <property type="nucleotide sequence ID" value="XM_017705161.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CM71"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019714.2">
        <property type="protein sequence ID" value="ENSPNAP00000012440.2"/>
        <property type="gene ID" value="ENSPNAG00000018236.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431791"/>
    <dbReference type="CTD" id="134510"/>
    <dbReference type="GeneTree" id="ENSGT00390000010107"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090364">
        <property type="term" value="P:regulation of proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01813">
        <property type="entry name" value="Ubl_UBLCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000060">
        <property type="entry name" value="ubiquitin-like domain-containing CTD phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000050">
        <property type="entry name" value="Ubiquitin-like domain-containing CTD phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011943">
        <property type="entry name" value="HAD-SF_hydro_IIID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051658">
        <property type="entry name" value="UBLCP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02245">
        <property type="entry name" value="HAD_IIID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48493">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING CTD PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48493:SF1">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING CTD PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="14">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="FCP1 homology" evidence="15">
        <location>
            <begin position="133"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012440.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="318" mass="36681" checksum="E8B0CB7BF74B2FA2" modified="2024-11-27" version="2">MSVSVIIKWGGQEYSISSLSEEDTVLDLKQSIKSLTGVLPERQKLLGLKVKGKPADDDTKLGTLKLKPNTKIMMMGSREESLEDVLAPPPENDDVVNDFDIEEEVIEVENREENLAKIARRVKEYKVEELNPPREGKRLLVLDVDYTLFDHKSCAETGQELMRPFLHEFLTSAYEDYDIVIWSATSMKWIDAKMKELGVTDNPNYKITFMLDSGAMITVHTPKRGIVEVKPLGVIWGKYGEFYNRKNTIMFDDIGRNFLMNPQNGLKIRPFMKAHLNREKDKELLKLSHYLKEIGKLDDFSGLNHKHWERYLSKKQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CM99</accession>
    <name>A0A3B4CM99_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-acetylaspartylglutamate synthase</fullName>
            <ecNumber evidence="5">6.3.2.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RIMKLA</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-acetyl-L-aspartate + L-glutamate + ATP = N-acetyl-L-aspartyl-L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40035"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16953"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:76931"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RimK family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.41" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CM99"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011951"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036326.2">
        <property type="protein sequence ID" value="ENSPNAP00000011951.1"/>
        <property type="gene ID" value="ENSPNAG00000017669.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014577"/>
    <dbReference type="OMA" id="DAAYNFN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072590">
        <property type="term" value="F:N-acetyl-L-aspartate-L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000011">
        <property type="entry name" value="beta-citrylglutamate synthase B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000022">
        <property type="entry name" value="beta-citrylglutamate synthase B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000014">
        <property type="entry name" value="beta-citrylglutamate synthase B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF0">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="115"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="406" mass="44490" checksum="6A209B61D0FB4A7B" modified="2018-12-05" version="1">MCSRVWFVTDRRISQEYPQVQILRALKERCAEDDVEFRYLLMDEIVITITNGQLGLRVGQEVVTSYPQVAVVRVPTPWVQSDSDITVLRHLEKMGCRLVNRPQAILNCVNKFWTFQELAGHGVPLPDTYSYGGHDNFRKMIDEAEPLGYPVVVKNARGHRGKAVFLARDKHHLADLCHLIRHEAPYLFQEYVKESHGRDVRVVLVGGRVIGSMLRCSTDGRMQSNCSLGGVGMMCPLSEQGKQLAVQVSNILGMDVCGIDLLQLNDGSFVVCEANANVGFIAFDQACGMDVAGIVADYALSLLPSRLNRKMSLLSVVSSASETSSEPEVCIPTEVCIPPEVCPPVATCAIPDAVSTMSTSSTSSESEAELTEIGHAPSAADPAYNINSLLAKEIKLLTEIKLVCVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMG2</accession>
    <name>A0A3B4CMG2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">HDAC5</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="RefSeq" id="XP_017556043.1">
        <property type="nucleotide sequence ID" value="XM_017700554.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMG2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012530"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036567.2">
        <property type="protein sequence ID" value="ENSPNAP00000012530.2"/>
        <property type="gene ID" value="ENSPNAG00000018413.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429074"/>
    <dbReference type="CTD" id="10014"/>
    <dbReference type="GeneTree" id="ENSGT00940000160534"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072114">
        <property type="term" value="P:pronephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10164">
        <property type="entry name" value="ClassIIa_HDAC5_Gln-rich-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10007">
        <property type="entry name" value="HDAC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000002">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046949">
        <property type="entry name" value="HDAC4/5/7/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF12">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037911">
        <property type="entry name" value="HDAC_II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="19">
        <location>
            <begin position="105"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="18">
        <location>
            <begin position="719"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="28"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="225"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="332"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="553"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1106"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="148"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="848"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="711"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="719"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="796"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Contributes to catalysis" evidence="15">
        <location>
            <position position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012530.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1133" mass="123498" checksum="E3730856BE01C515" modified="2024-11-27" version="2">MLLRPTVPGLCAMLQTIYETESCFSTDAHSTASREQPTELLPHSTTNNMSSTAVEVKSSLSSGMQSPVGGGGAEQGGGGGGGGGGPVDLRTELRADPRLSALSGMDPALREQQLQHELLLLKQQQELQKQLLFAEFQKQHEVLTRQHEAQLQEHLKQQQELLAVKRQQELEQKRKLEQQRHEELEKQRLEQQLIMLRNKEKGKESAIASTEVKLKLQEFLLSKKEPPSGGLNHSFPQKCWGAHHTSLDQSSPPQSNTPGTPPSYKLPPLLGAYEGKDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAIEISVSSMCSSAPGSGPSSPNSSNSAIAENGSSGSVPNIHAEQLRSLHQSLNADGTVSPLSLYTSPSLPNISLGLPANAHITAPQKLTVQQDAERQALQSLRQGGALTGKFVSTSSLPAHDPDPPAPTNSHSPHSSLLQHVLLLEQARQQSALLAVPMYGKSPLVTGERVSNNMRTVTKLPRHRPLSRTQSAPLPQTPQALQHLVMQQQHQHFLEKQKHYQLNKILSKGAELPRQPPTHPEETEEELTETAEMPDERGEGPEHVREGLHKESSGETTPPSERVVTLKGESTESDLEEEDDEDEAIELKECDEEAGPYGQFLEQQHLQQLNVFQASLSITGMPHRPLGRAQSSPITSSLKGTPMSELPIKHLFTTGLVYDTLMLKHQCMCGNTHIHPEHAGRIQSVWSRLQETGLLGRCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSGAVRMAVGCVIELAFKVAAGELKNGFAVVRPPGHHAEESTAMGFCFFNSVAVTAKLLQQKLGVGKILIVDWDIHHGNGTQQAFYNDPNVLYISLHRYDDGNFFPGSGAPEEVGVGPGVGFNVNIAWTGGVDPPMGDVEYLTAFRTVVMPIANEFSPDVVLVSAGFDAVEGHQSPLGGYSVTAKCFGHLTKQLMKLAGGRVVLVLEGGHDLTAICDASESCVAALLGDELDPLPQTVLQQKPCPKATASLERVIEIQSEHWASVQRSAPTVGQSLLDAQRREKDEADTVTAMASLTVDTEQSAASSQSSRAEEPMEEEPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNN1</accession>
    <name>A0A3B4CNN1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037393290.1">
        <property type="nucleotide sequence ID" value="XM_037537393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNN1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020371.2">
        <property type="protein sequence ID" value="ENSPNAP00000012945.2"/>
        <property type="gene ID" value="ENSPNAG00000019278.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437538"/>
    <dbReference type="GeneTree" id="ENSGT00940000161510"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000001">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="15">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="16">
        <location>
            <begin position="120"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="666"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="12">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012945.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="666" mass="76345" checksum="EFE7B8245E7E0423" modified="2024-11-27" version="2">MGEYKIQVFTGTVLGAGTSNKIYINLIGSISDSGPHKLKTLTAFWPGSRQTFTVESESNIGELLLVEVEPRPRSLLPYVDDDWFCTKIIVTTPEGVDVLFPCYCWISRDEKLILRHSIAMLKTKDTVPQLLAHRQENLKKRRQEFKWSVYAPGLPHTVLATLPHELPAEVRFSFTREKEFLFTSGAAFTKLKLENPLNGTWGSFEEIKKILSKKPKTYEYVEKNWMTDEFFAYQFLNGLNPMIIKRCSQLPEKFPVTNEMVKCFLPGTSTLESEMKKGNIFLCDYERLRDLEGNQICKKKQYLAAPLCLLFSTQDKLMPIAIQLNQQPGPGNPIFLPSDSENDWLLAKIFVRNAEFNEHELNFHLLRAHLMGEVFTVATMRNFPLCHPLFKLLIPHTRFNLQINILARRLLISETGYFPKFTAIGNKSMTTFLKRAASSVTYSSLCIPDDIKERGLETIPNNYYRDDGLELWDIIYKFVSGFVSHYYRDQDVKDDNELHSWLTEIRENGFLERSEAGFPKSFCTVKELVKFVTMVIFTVSGQHAAVNSSQFEFGGFMPNFPSSLRCPPPRQKGETTMDSVLETLPDINTTINTVAIVYLLSQESADRYPLGHYPEELFSEEVPLKLIAQFKNALQDLEMKIDNRNKKLALPYTYLNPPNVDNSVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSF9</accession>
    <name>A0A3B4CSF9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inhibin beta B chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Activin beta-B chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">INHBB</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CSF9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014293"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037271.2">
        <property type="protein sequence ID" value="ENSPNAP00000014293.1"/>
        <property type="gene ID" value="ENSPNAG00000020195.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159862"/>
    <dbReference type="OMA" id="RMDGDFL"/>
    <dbReference type="OrthoDB" id="6516235at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19405">
        <property type="entry name" value="TGF_beta_INHBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000005">
        <property type="entry name" value="Inhibin beta A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000012">
        <property type="entry name" value="inhibin beta B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000381">
        <property type="entry name" value="INHBB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF29">
        <property type="entry name" value="INHIBIN BETA B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00671">
        <property type="entry name" value="INHIBINBB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-0372">Hormone</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017203913" description="Inhibin beta B chain" evidence="14">
        <location>
            <begin position="21"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="15">
        <location>
            <begin position="271"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="389" mass="43561" checksum="CD2BCA207BADD54A" modified="2018-12-05" version="1">MRKLVVKVLCFAACVLSSRCTPAPDTEAQTVSPRDTCASCGLGQPDESRRMDGDFLEAVKRHILSRLQMRDRPNITHPIPKAAMVTALRKLHAGKVREDGRVEIPNLDGHASYTNEVEEETSEIISFAETDELLSSKSSLFFVISNEGNQNLYVSQANLWLYFKLLPISSEKGSRRKVTVKVYYQEPGLGSKWNLVEKRVDLKRSGWHTFPLTDAVQLVFSKGERRQNLDIRCEGCEAAGVLPALVNSADEAHRPFLVVRARAVEDSKHRIRKRGLECDGSSSLCCRQQFYIDFRLIGWNDWIIAPSGYFGNYCEGNCPAYMAGVPGSASSFHTAVVNQYRMRGMSPGSMNSCCIPTKLSTMSMLYFDDEYNIVKRDVPNMIVEECGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CT53</accession>
    <name>A0A3B4CT53_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mannosidase endo-alpha like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MANEAL</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 99 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CT53"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014533"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022507.2">
        <property type="protein sequence ID" value="ENSPNAP00000014533.1"/>
        <property type="gene ID" value="ENSPNAG00000020460.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016054"/>
    <dbReference type="OMA" id="FHMSSSD"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11574">
        <property type="entry name" value="GH99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000019">
        <property type="entry name" value="glycoprotein endo-alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026071">
        <property type="entry name" value="Glyco_Hydrolase_99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572">
        <property type="entry name" value="ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572:SF2">
        <property type="entry name" value="GLYCOPROTEIN ENDO-ALPHA-1,2-MANNOSIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16317">
        <property type="entry name" value="Glyco_hydro_99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="49735" checksum="0AF997076F5150BB" modified="2018-12-05" version="1">MSRLRKKACVALFLFTLFIFGTMMGLRTLKPSDGFSDLAPGMERAPFAAERAERRAETESDVSDTKIVFSNSGHEHSIFYDIHIFYYLWYGAPQTDRGYIHWDHVLVPHWDPKIAASHPKGRHSPPEDIASSFYPELGPYSSRDPHILESHMAQIEAAAAGVVVLSWYPPGVADEHGEPTEDLVPAVMDAAHRHRIKVAFHIQPYRGRTELSMHENIKYIIDRYGKHGAFYRFKSSTGKVLPLFYVYDSYLTPPEAWAELLTSRGSHSLRGTPYDGIFIALIVEERHKHDILASGFDGMYTYFASNGFSFGSSHQNWKAVKAFCDRNNLLFVPSAGPGYVDTAVRPWNNHNTRNRVNGRYYETSLQAALSVRPDIITITSFNEWHEGTQIERAVPKKTVTRLYLDYKPHQPDLYLELTRKWAEQFSKEKEQWLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4Q0</accession>
    <name>A0A3B4D4Q0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibroblast growth factor</fullName>
            <shortName evidence="4">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D4Q0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019327"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029055.2">
        <property type="protein sequence ID" value="ENSPNAP00000019327.2"/>
        <property type="gene ID" value="ENSPNAG00000006259.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158058"/>
    <dbReference type="OMA" id="DESTQFN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23309">
        <property type="entry name" value="beta-trefoil_FGF11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000001">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019327.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="248" mass="28000" checksum="AA3B7A0D5779E95B" modified="2024-11-27" version="2">MAALASSLIRQKRAVKDDQANRPIANKRKPCPKSNKSLCQKQILVLISKVRLCGGRRGRTDKRPEPQLKGIVTRLYSQHGYYLQMQPDGTMDGTRDESSSFSQFNLIPVGLRIVAIQGAKTGLYLGMNSEGYLYTSEHFNPECKFKESVFENYYVTYSSMLYRQTQSGRSWYIGINRDGQVMKGNRVKKTKPASHFLPKVIEVAMYKEPSLHELVLEQSPPRKTVKTSDSPSHQNGHKEAGLRVPCAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5V8</accession>
    <name>A0A3B4D5V8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA (adenine(37)-N6)-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA methyltransferase O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TRMO</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + S-adenosyl-L-methionine = N(6)-methyl,N(6)-L-threonylcarbamoyladenosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70027"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:17808"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:188470"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:70028"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5V8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018900"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039201.2">
        <property type="protein sequence ID" value="ENSPNAP00000018900.1"/>
        <property type="gene ID" value="ENSPNAG00000025379.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004643"/>
    <dbReference type="OMA" id="IDMIQGT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.70:FF:000002">
        <property type="entry name" value="tRNA (Adenine(37)-N6)-methyltransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2310.10:FF:000002">
        <property type="entry name" value="tRNA methyltransferase O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.10">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TsaA-like" evidence="10">
        <location>
            <begin position="82"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="10"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="454" mass="50209" checksum="749F3E862668D694" modified="2018-12-05" version="1">MAPCSCSEQIRRLSQQVAVMRKEMKNLRQHLESAVRAHRKHMASLQSELEACRQGKHEMKKTAEKDPQRGAEFSLEKGHIQTVPIGYISSCFSKKNGTPRQPAVCMSSRAALQIQPSVFNNPDHALAGLEHYSHVWLIFLFHKNGHMSYKAKVKPPRLNGQKVGVYSTRSPHRPNAIGLSLAKLESITGDTLHLSGVDLIAGTPVLDIKPYIPDYDSPSAQTNMSVQYETTEDAGDSEPEGLQCLGDSSSELDPKMRFSASGGLSEHSGSVFSEVLAEVRDYLQQGALCVETLADRRPSEPEPEPESQRASRLCYGADSYSSVAAWIRNPPAAELSVRFTPTAEEELREFQPPESTDAGRPKFQFLKGPDEAAAAVRGILSADPRSVYRRTRCSDRLFYFSLDTADVTCWFGDGFAEVLRVRPVQTQECLTTTRHSAARVIAGRASESEEELNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6R4</accession>
    <name>A0A3B4D6R4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transferrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017555696.1">
        <property type="nucleotide sequence ID" value="XM_017700207.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6R4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027918.2">
        <property type="protein sequence ID" value="ENSPNAP00000018619.2"/>
        <property type="gene ID" value="ENSPNAG00000025070.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428860"/>
    <dbReference type="CTD" id="565818"/>
    <dbReference type="GeneTree" id="ENSGT00940000166763"/>
    <dbReference type="OMA" id="ACIGDDN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048840">
        <property type="term" value="P:otolith development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd13529">
        <property type="entry name" value="PBP2_transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000095">
        <property type="entry name" value="Lactotransferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001156">
        <property type="entry name" value="Transferrin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485:SF49">
        <property type="entry name" value="OTOLITH MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485">
        <property type="entry name" value="TRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00405">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00422">
        <property type="entry name" value="TRANSFERRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00094">
        <property type="entry name" value="TR_FER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51408">
        <property type="entry name" value="TRANSFERRIN_LIKE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044005747" description="Transferrin-like domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="4">
        <location>
            <begin position="28"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018619.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="372" mass="40641" checksum="A71BB0CC7BA9566C" modified="2024-11-27" version="2">MDQTTGHLLALAFLLLVKVGMSSQNNIITWCAVSDAEEQKCLDLAGNATAQNIRGKLQCVRGQSPTDCMQRIKNGTADAASMYPDEIYTAGVCYGLDVAVGESYNGMDGINYYVVALARRSSGDLSLLEMHERSSCHPGIRTTVGWTVPIGFLVNTSQISVNEQCNFPHAVGDFFGYSCVPGAKDPEHDPKGTNPRNLCEACIGDENDRHICANNPRERHYGEAGALRCVAENLGDVAFVKHTTVFDNMNGKNQESWALDLELEDLKLLCPDGGEASPSQHKHCHLAVVPANAVVVRLEDKCRVYKFLERVQNVFANSTQGFSLFSSAGYGQPDVMFSDSTQKLLRVMGTYPSWLGPSYTTMLQAFECEGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D809</accession>
    <name>A0A3B4D809_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017577619.1">
        <property type="nucleotide sequence ID" value="XM_017722130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D809"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019084"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028686.2">
        <property type="protein sequence ID" value="ENSPNAP00000019084.1"/>
        <property type="gene ID" value="ENSPNAG00000025597.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442199"/>
    <dbReference type="CTD" id="338872"/>
    <dbReference type="GeneTree" id="ENSGT00940000154936"/>
    <dbReference type="OMA" id="NWLYADE"/>
    <dbReference type="OrthoDB" id="5875591at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF21">
        <property type="entry name" value="COMPLEMENT C1Q AND TUMOR NECROSIS FACTOR-RELATED PROTEIN 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C1q" evidence="7">
        <location>
            <begin position="224"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="359" mass="37936" checksum="45591A84E501DE5E" modified="2018-12-05" version="1">MLWFWSRISRPYLVVRIMSSAKQFFILLLVALVHVAEQESSGRREKSDDCVCGHPGIPGNPGHNGMPGRDGRDGAKGDKGDPGDIGTCGATGKDGPKGDKGEPGAPGIDGVKGRRGENGERGPPGKMGPQGFPGPLGLKGLKGEMGMPGPQGIKGDVGPLGPEGPQGIIGYKGEKGVQGPIGPPGRPGPKGDLGPPGHKGSIGYRGDKGSKGEIGEKGPQGDMPEIPKSAFSAGLTAQSKLPASNVPIRFEKIIYNRQNHYDPQSGRFTCAITGAYYFTYHITVFSRNVRVALMKNGQSIIHTMDNYQNNEDQASGGTVLYLQAGDKVWLQVVGEQFFNGLYADDDDDTTFSGFLLFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D932</accession>
    <name>A0A3B4D932_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">C5orf49</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D932"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019943"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030134.2">
        <property type="protein sequence ID" value="ENSPNAP00000019943.1"/>
        <property type="gene ID" value="ENSPNAG00000006205.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015121"/>
    <dbReference type="OMA" id="RKNGIAC"/>
    <dbReference type="OrthoDB" id="10057935at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027901">
        <property type="entry name" value="CFAP90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34444:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34444">
        <property type="entry name" value="LOC361192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15074">
        <property type="entry name" value="CFAP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="137" mass="15931" checksum="395DA0B52E91AA43" modified="2018-12-05" version="1">MDVLSEAKVRPLSTLSVFSFIPPRRSDTKERRYFNCIPKAAEVFLYDCIHRRVEGYDNKLHRDDRQHAKHCGLDIFSEESSRPIAVLSSSEYGRRVQPLLYKPARQFARVAHIRSEFYRKNGITRTLEEGYGSVVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBR7</accession>
    <name>A0A3B4DBR7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger, AN1-type domain 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ZFAND4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017580841.1">
        <property type="nucleotide sequence ID" value="XM_017725352.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017580849.1">
        <property type="nucleotide sequence ID" value="XM_017725360.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBR7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020521"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031156.2">
        <property type="protein sequence ID" value="ENSPNAP00000020521.1"/>
        <property type="gene ID" value="ENSPNAG00000007097.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444269"/>
    <dbReference type="CTD" id="93550"/>
    <dbReference type="GeneTree" id="ENSGT00940000155716"/>
    <dbReference type="OMA" id="ECRCGHN"/>
    <dbReference type="OrthoDB" id="756206at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01802">
        <property type="entry name" value="Ubl_ZFAND4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053061">
        <property type="entry name" value="AN1-type_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46728">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46728:SF1">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="28"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="7">
        <location>
            <begin position="645"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="260"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="77253" checksum="871B4CAF464D2805" modified="2018-12-05" version="1">MADKKDPPFFNDDNFGVVHYKLPFYETMELFIETLTGTCFQLRVSPFETVVSVKAKIQRLEGIPVAQQHLIWNSLELEDEYCLHDYSIAEGCTLKLVLAMRGGPINTRRVTVDESVKDTSDCLDSRRDEVWEKSLSNKQVTFLVYREGDQLNFFRVVDRGDGTLTPVSESLSGGSVHNVYAEEEEDGVSNSSGQLSLENSITMNKMKLLKAKMENMNLNKKPKKTAKLKVRPPVGPRPCSSSLGSVRHHRMFRVLPQISHGSATNLPPVGDQQQPATPSSAAGPYSTPLTSHLSPASSRAVPSLTASSIYMLQEEEPWNNPTPRKIRMPPKMSRLDVSGSKLIRDCVYPPLSVLSGQDEAEPQRDRAALAEDAVVIEPPKPVPFNSLLPEPLSLDVSTQRDASLDSLGAVAEPMAAPPLLSQAVGSDSVSTWAIGTKTLQAQTDSDTWVIGTKTLQSQPALHLSTEPLTPPRMPQPFEFTGASLRVSPSHTLLSSNDTPPVPPRSPSQGIPLCGIKVDSPGKRLEVISNSEARDITKTANKSSKELLGCVSNTELLASLAGSRGKESMAGPCALGRLCGASAPLPASIHLLQEDLLHRLNPLPKAAAYMPSGILGSLSSPSSSIKRQGTPTYHLPPVKAPVGSKKKTSKHCFMCGKKTGLATSYECRCGNNFCSTHRYAETHDCTYDYKSAGRRFLQEANPIISAPKLPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDU5</accession>
    <name>A0A3B4DDU5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:ch211-263p13.7</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDU5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033155.2">
        <property type="protein sequence ID" value="ENSPNAP00000021660.2"/>
        <property type="gene ID" value="ENSPNAG00000005229.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008727"/>
    <dbReference type="OMA" id="CHIEHAE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026505">
        <property type="entry name" value="Solute_c_fam_35_mem_F3/F4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346:SF2">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021660.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="366" mass="40034" checksum="60859DC8A0703D0B" modified="2024-11-27" version="2">QPVRSCCSRCPLKVLLRQTTGLLLASCVSAAWVWAAHSAKQTLTRLHAPFFIIWFCGIWNLLFFPLYYLGHLLGSERREWPSVQLRKCSRFLGDGDLTLRLVLKGAAPFSLLWSVSGYLHLLSLCRISISDSSAVLCCSHAFAFLLSWIGLKDRFMGVRIVAAILSITGIVMLAYADGFHSNSITGVALGVGSASTLAFYKVLFRKRVCEAGPGGVCVLLSCVGVCNLLLHSWLCVLLYLTHTEYWSPTQQSVPWSSLCTTAALLLLFNVLVNLGGVVSYPALISLGILLTIPASADVHKFNVRGAAFGIISAGFLLLLLPEDWDERALRWFGTLWHDRDESVLGEDWGADTPRPKPKPAGVAALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJ23</accession>
    <name>A0A3B4DJ23_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJ23"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013406.2">
        <property type="protein sequence ID" value="ENSPNAP00000023126.2"/>
        <property type="gene ID" value="ENSPNAG00000000396.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162219"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000153">
        <property type="entry name" value="MUC5B isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF408">
        <property type="entry name" value="MUCIN-5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="312"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023126.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="377" mass="42271" checksum="BC60F272A95B65EA" modified="2024-11-27" version="2">NHNMQVCSTWGNFHFSTFDGQFFQLPYTCNYILTTLCDSTKTDFNIQMRRQYINNLPTIRSFTIKIDGVVVNECCTLVIGYGAIHFCIVCKLYESSVCCITPAQVEIPTKYRNQTCGLCGDFNGVKDNEFIENGDFDFFHPSQNYLCEQLLSDPAFSNCHRLLSIEAFVEACVRDVCQCNSSQALCLCDTISEFSRQCAHAGGKPQNWRTNYLCGKECPFNLEHKECGSPCKDTCSNQEGSLVCNEHCSDGCFCPAGTVFNDIEENGCIPVNKCPCIHNGRIYQPGESYNRTCQKCVCSEGRWSCVNLDCPGTCSIQGGSHITTYDGKAYNFHGNCYYVLSKVTIPTFVKLCNIITNFIISETSVSISKYLMVYGHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJ74</accession>
    <name>A0A3B4DJ74_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">D-arabinitol 2-dehydrogenase [ribulose-forming]</fullName>
            <ecNumber evidence="10">1.1.1.250</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="9">Carbohydrate metabolism; D-arabinitol metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.250" evidence="10"/>
    <dbReference type="RefSeq" id="XP_017547265.1">
        <property type="nucleotide sequence ID" value="XM_017691776.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJ74"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023503"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012458.2">
        <property type="protein sequence ID" value="ENSPNAP00000023503.1"/>
        <property type="gene ID" value="ENSPNAG00000008064.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424032"/>
    <dbReference type="GeneTree" id="ENSGT00940000165309"/>
    <dbReference type="OMA" id="IFACAET"/>
    <dbReference type="OrthoDB" id="1669814at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047038">
        <property type="term" value="F:D-arabinitol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000240">
        <property type="entry name" value="SDR family oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000707">
        <property type="entry name" value="Zgc:113054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF115">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="550" mass="60656" checksum="76E45F2DD3456676" modified="2018-12-05" version="1">MQVDKVSDTPLEFIVEYLLKAKEIADRNASVPEELRCNLQKALDIASGLDMYLERMSSPESEPLAELFKKTVSHEWNKVYEDGKTMFRLPKECITGHVEGQFLKMLVHMSKARKVLEIGMFTGYGALSMAEALPEDGQLIACEIEPYLKDFVQPVFDKSPHGKKITVKVGSAMESLKEFARAGEQFDMVFIDADKNNYLSYYTFLLDNNLLRMDGVLCVDNALFKGTVYLNDTTDQNGLTLREFNQFVASDPRVEQVVIPLRDGVTIIRRVPYSPEMDAQNTVTYDEVFRGVQGKTILSRLRLDGKVAYVTGAGQGIGRAFAHALGEAGAKVAIVDMDKGKAERVTQELGLKGISSMALAADISKPEDVEKMIDSIISKWGAVHIACNNAGINMNSASEDTSLSEWDQTFNVNLRGAFMCCQAAGRVMLKQGYGKIINTASMASLIVPHPQKQLAYNTSKAGVVKLTQTLGTEWIDRGVRVNCISPGIVDTPLIHSESLEPLLQRWLSDIPAGRLAQVTDLQAAVVYLASEASDYMTGHNLVIEGGQSLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJS1</accession>
    <name>A0A3B4DJS1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum membrane sensor NFE2L1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Nuclear factor erythroid 2-related factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Nuclear factor, erythroid derived 2, like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">NFE2L1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bZIP family. CNC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJS1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001582"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011919.2">
        <property type="protein sequence ID" value="ENSPNAP00000001582.1"/>
        <property type="gene ID" value="ENSPNAG00000008250.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426461"/>
    <dbReference type="CTD" id="558772"/>
    <dbReference type="GeneTree" id="ENSGT00950000182892"/>
    <dbReference type="OrthoDB" id="7458135at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14720">
        <property type="entry name" value="bZIP_NFE2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.880.10:FF:000001">
        <property type="entry name" value="Nuclear factor erythroid 2-related factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.880.10">
        <property type="entry name" value="Transcription factor, Skn-1-like, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047167">
        <property type="entry name" value="NFE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF31">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE SENSOR NFE2L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411">
        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="21" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="114"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="289"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="488"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="608"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="781"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="707"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="501"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="791"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="800" mass="88171" checksum="CA247D591CE71525" modified="2018-12-05" version="1">MLYLKKYFTEGLIQFTILLSLIGVRVDVDTYLSNQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSVDLDHFFAIRQLLNQVHSLDHLRVPSTELSAWLVHRDPETVVSATSQSGPSIALDNGGSLEDVNNPEASAMRGAGSASESTYSLSGEDSLGAVAPENSQEQADREDNDDLSKEDIDLIDILWRQDIDLGAGREVFNYSNRQKDGEADKPNEEHEEASVREDWRNGINLQEGQSLTVVDGETGESIPEQLSSLGAQTSMSLQECLRLLEATFPFGEESEFQATDAAPQLSGSTEDVPSTSQRLPLPDSLPQSDVPLDLEQQWQDIMAIMELQDMDVNNTAENSTLNSQNNTGPNTTESGTVANFGLSRSSLINQDVSLHQASLPSCSQDLPSLFNPELDPSAGQRPALLRLASSNSSNINSTFGATNLTGLFLPPLLNSTSNLTSTPVLPDPFTSLLEESMLDEISLLDLAMEEGFSQAQASQLEHELDSDSGLSLDSGHSPVSPSNSETSCSSAASTSSTSATFSEEGAVGYSTDSEAATAEAEEGAVGGYQPEYSKLCRMSYQDPSHFHGLPQMDSINHNHTYNLPLASSSYPEHSQLLGSCSKKGRDKQLQQTKLQPPRDFIDKQSSRDERRARAMKIPFSNDKIVNLPVEEFNELLSKHHLSEAQLSLIRDIRRRGKNKMAAQNCRKRKLDTIINLEKGVQDLRRDKARLLKEKVEYLRCIRQMKQKVESLSQEVLSQLRDEEGRPYPASEYSLQYGTDGSVLIMPRSMAAEEGRKPDKKQKDKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKD2</accession>
    <name>A0A3B4DKD2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Choline uniporter that specifically mediates choline uptake at the blood-brain-barrier. Responsible for the majority of choline uptake across the blood-brain-barrier from the circulation into the brain. Choline, a nutrient critical for brain development, is a precursor of phosphatidylcholine, as well as betaine. Also mediates transport of ethanolamine. Choline and ethanolamine transport is not coupled with proton transport and is exclusively driven by the choline gradient across the plasma membrane. Also acts as a heme b transporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>choline(out) = choline(in)</text>
            <dbReference type="Rhea" id="RHEA:32751"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ethanolamine(in) = ethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:32747"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>heme b(in) = heme b(out)</text>
            <dbReference type="Rhea" id="RHEA:75443"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the major facilitator superfamily. Feline leukemia virus subgroup C receptor (TC 2.A.1.28.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546346.1">
        <property type="nucleotide sequence ID" value="XM_017690857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKD2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009099.2">
        <property type="protein sequence ID" value="ENSPNAP00000024842.1"/>
        <property type="gene ID" value="ENSPNAG00000009615.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423509"/>
    <dbReference type="CTD" id="565210"/>
    <dbReference type="GeneTree" id="ENSGT01030000234625"/>
    <dbReference type="OMA" id="GLCQNFM"/>
    <dbReference type="OrthoDB" id="422206at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015220">
        <property type="term" value="F:choline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097037">
        <property type="term" value="P:heme export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000101">
        <property type="entry name" value="feline leukemia virus subgroup C receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000092">
        <property type="entry name" value="Feline leukemia virus subgroup C receptor-related protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF3">
        <property type="entry name" value="HEME TRANSPORTER FLVCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="350"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="18">
        <location>
            <begin position="58"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="495" mass="54848" checksum="792B397D2F127750" modified="2018-12-05" version="1">MCDCDNSEDVKGEKNGPVPVESNISTSEHVGDHIECKEISSENPQVRSTRLYKRRWAIVFLFSSYSLCNAFQWIQYGIINNIFMHFYSVSSFAIDWMSMIYMLTYIPFIFPVTWLLDKKGLRVVALVASSINCLGTWIKVASVRPHLFWVTMLGQFACSFAQVFILGMPSRIASVWFGSGEVSTACSIGVFGNQLGIAIGFLVPPILVPNVEDLDELAYHISIMFYITAGVATLIFILVIIVFQEKPDVPPSQAQAAMRNMSPQEYSYLASIRRLVCNKPFVLLIITYGLNVGCFYAVSTLLNRMIIDHYPGEEVNAGRIGLIIVIAGMVGSLICGIWLDKTKTYKQTTLAVYLLTFVGMLVYAFTLNLGHLWIVFITAAALGFFMTGYLPLGFEFAVELTYPESEGTSSGLLNCSAQVFGIIFTICQGKIIDYYGTLAGNIFLCVFLFLGTLMTAFIKADLRRQKANQLAVREAAAKDSGCQDGGLLPLKEAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKD8</accession>
    <name>A0A3B4DKD8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARFIP1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017564854.1">
        <property type="nucleotide sequence ID" value="XM_017709365.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017564855.1">
        <property type="nucleotide sequence ID" value="XM_017709366.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKD8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023923"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011425.2">
        <property type="protein sequence ID" value="ENSPNAP00000023923.1"/>
        <property type="gene ID" value="ENSPNAG00000000972.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434328"/>
    <dbReference type="CTD" id="27236"/>
    <dbReference type="GeneTree" id="ENSGT00950000183040"/>
    <dbReference type="OMA" id="QAEKGSM"/>
    <dbReference type="OrthoDB" id="9994780at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07660">
        <property type="entry name" value="BAR_Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000003">
        <property type="entry name" value="arfaptin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF4">
        <property type="entry name" value="ARFAPTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="7">
        <location>
            <begin position="260"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="169"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="480" mass="53181" checksum="91CC872D1834967D" modified="2018-12-05" version="1">MSEASFESQEKRSDPEEDAPDNGTQSQGQSEDTPGSFSDDTHMTEIDINDSSESRTFDRSLNDEDAEDGAAGQSKSNNNGVHKDPEKTNTASTDAVPSQSAAKVAMAEEAHRSPAAELSVTSNGDADTGHEEVFQRDLSHGVSSGPITSHTHNAPDSYGMMTEGVVEVGPYTGSKSQPTSPTIAPSPVAASRLARTASDSQVETEKGSMRGQQQSGSVVLADDLKNPAMEKLELVRKWSINTYKCTKQILSEKLGRGSRTVDLELEAQIEVLRDNKRKYEHVVKLAQTLANQLAQMMQTQRQLGDAFADLSLKSPELHEEFGYNAETQKLLSKNGETLLGAIQFFIASVNTLVDKTIEDTMINIKQYEAARIEYDAYRTDLEELNLGPRDATTLPKIEQSQMHFQIHREKYEKMRNDVSVKLKFLEENKVKVLHNQLILFHNAIAAYFAGNQQHLEQTLKQFHIKLKMPGGDIPSWLEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPX7</accession>
    <name>A0A3B4DPX7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger and BTB domain containing 45</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017539940.1">
        <property type="nucleotide sequence ID" value="XM_017684451.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPX7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007422.2">
        <property type="protein sequence ID" value="ENSPNAP00000025510.2"/>
        <property type="gene ID" value="ENSPNAG00000010663.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412436"/>
    <dbReference type="CTD" id="84878"/>
    <dbReference type="GeneTree" id="ENSGT00940000160859"/>
    <dbReference type="OMA" id="MVAWKGD"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18229">
        <property type="entry name" value="BTB_POZ_ZBTB45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000304">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000315">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF22">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="34"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="565"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="447"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025510.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="66819" checksum="CE66081B93F6D91B" modified="2024-11-27" version="2">MSSGSETVHYIHLHNASQSVLEALRTQRRKGLFCDVTVRIHDASLRAHACVLAAASPFFQDKLLLGHSEISVPPLVPAETVKQLVDFMYSGSLVVLHSQALCILTAASILQIKTVIDECTQIISQKRPLGAAMPKQEEQGGGKGRESVGNVGGVGASALQGYSYPIGDCGAGVGSAPGLQDPQPMGGGGQPDSSGLDGIMPPLGDLLGRGDGLGAPGGGPMMKPTSCTQEINYMLNQNAERSSAGAGQNQSQNQNMSDPGRAHMNTPAPTAMGRDGLRGNGGAEFVEELAVGMERFNEEEDVEERERDGERERGVGHSRKQRQPLRLQVLGGEQVVVKDESVQDGDNLFDLEERRDAHDNSHQSAYGQGGFYEEQAVFAGGFWPQTDASQTLVANPRSRSNKPLSPPTSSHNINNQMLFQYPVSESQPSSFFVGGPMVIDTMADTCQQAPPPAPLTPAPPPPAPPCMAGPSFTPQSSETSFDCSHCGKSLRSRKNYSKHMFIHSGQKPHQCSICWRSFSLRDYLLKHMVVHTGVRAFQCSVCSKRFTQKSSLNVHMRTHRVERTFSCNVCHRAFTHRTLLERHALQHQHPPIKPNNIDQAGGAGMGGAGMGAGMGGANGAGSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQP5</accession>
    <name>A0A3B4DQP5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Hairy/enhancer-of-split related with YRPW motif protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HEY1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HEY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548119.1">
        <property type="nucleotide sequence ID" value="XM_017692630.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQP5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025274"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008027.2">
        <property type="protein sequence ID" value="ENSPNAP00000025274.1"/>
        <property type="gene ID" value="ENSPNAG00000010284.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424539"/>
    <dbReference type="CTD" id="23462"/>
    <dbReference type="GeneTree" id="ENSGT00940000157068"/>
    <dbReference type="OMA" id="QIPWGGA"/>
    <dbReference type="OrthoDB" id="6371181at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021984">
        <property type="term" value="P:adenohypophysis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000012">
        <property type="entry name" value="hairy/enhancer-of-split related with YRPW motif protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="48"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="12">
        <location>
            <begin position="125"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="34167" checksum="176999F5DBC47C21" modified="2018-12-05" version="1">MKRNHDFSSSDSELDENIEVEKESADENVCMSSPLGSVSPSTTSQVQARKRRRGIIEKRRRDRINNSLSELRRLVPSALEKQGSAKLEKAEILQMTVDHLKMLHAAGGKGYFDAHALAMDYRGLGFRECLAETARYLSIIEGLDNTDPLRIRLVSHLNNYATQREAHTGLGHLAWGSAFGTPPSHLAHHLLLPQHQQGAPLSRSSSSPASSSSSSSSSSSSSPSAPSSEAHSSSRLSGTVIAEAGQAGPLRVTPGTPLPPGLTAASASKLSPPLLTSLSGLSAFPFPLSAFPLLSPGSLGPATPSSSLGKPYRPWSMEIGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUT7</accession>
    <name>A0A3B4DUT7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KRT4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUT7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028152"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000721.2">
        <property type="protein sequence ID" value="ENSPNAP00000028152.2"/>
        <property type="gene ID" value="ENSPNAG00000008621.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161090"/>
    <dbReference type="OMA" id="MHATITP"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF21">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="129"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="133"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="338"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028152.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="520" mass="55865" checksum="CDE7D35D6742DB89" modified="2024-11-27" version="2">MASTRQVSYSFKGPSSASVRKSYSTQSAYAIPSVGSSRVSTVRRSVVGAGPVYGGGFSSSSIGGGVSGAYGFGSGLSAGFGGGYGGGLGGGFGGGFVPAPITAVTVNQSLLAPLNLEIDPTIQAVRTQEKEQIKTLNNRFASFIDKVRFLEQQNKMLETKWSLLQEQTTTRSNIDAMFEAYIANLRRQLDGLGNEKIKLEGELKNMQGLVEDFKKKYEDEINKRAAAENEFVLLKKDVDAAYMNKIELEAKVDALQDEINFLRAVYEAELHELQSQIKDTSVVVEMDNSRNLDMDAIVAEVRAQYEDIASRSRAEAESWYKQKYEEMQTSAGQYGEDLRTTKAEIAELNRMIARLQNEIEAVKGQRANLEAQITEAEERGELAVKDARLRIKDLEEALQRAKQDMARQVREYQELMNVKLALDIEIATYRKLLEGEESRISAGGSSATIHVQQTSGGGGFSSASSGFGYGGSYGGGYGGGIGGGIGGGIGGGIGLSGGTSLSGSSVITKSSSKTISAKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVT2</accession>
    <name>A0A3B4DVT2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DVT2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027176"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003255.2">
        <property type="protein sequence ID" value="ENSPNAP00000027176.2"/>
        <property type="gene ID" value="ENSPNAG00000002318.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282522"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF170">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="23"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027176.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="295" mass="33536" checksum="15357781A5A7A337" modified="2024-11-27" version="2">MAFNDPLLPKHLSSREPSKMSELRIILMGKDGPENSRVGNTILGRDAFETESPPSVQQHSERARGKVQGRYITLINTPCLFETKLGQEELTWRVEESVFLCAPGPHVIVLILEPDSFTQIDRNRAHTVLRYLSEEARKYTMVLTTQIMETGTSVDAESTVQMLIAECSNRYLELSRFSSAEFVKMLEEIVHVNEGNLTCEVYKEAPLATESWHRFEDLEDEEAKLETNKHKEAEEHIELKKPTGKIRVFSKVKTAVLSTSSAFRKNVCAPKPRLNVMLFGREEETKALISNLLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DWP2</accession>
    <name>A0A3B4DWP2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DWP2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001335.2">
        <property type="protein sequence ID" value="ENSPNAP00000027913.2"/>
        <property type="gene ID" value="ENSPNAG00000034310.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163299"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000013">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="4"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="95"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="198"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="29"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="42"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="119"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="132"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="223"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="236"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="344" mass="37278" checksum="A76B887FEF0D4E7F" modified="2024-11-27" version="2">GVSVRLMDGGSRCAGRVEVLHRGQWGTVCDDNWDMRDAAVVCRELGCGEAVDALSDAHYGPGSGPIWMNNMSCSGSETTLKNCRSDGWRKHNCKVRLVGGSHCSGRVEVLHGGTWSTVCDATFDQQDAEVVCRELGCGLPVEVLEAAAFGRGEGQVWSEQLQCRGNESQIHFCPTSSSLKHNCSHDSDVGLVCAGKSVRLVDGGSRCAGRVEVLHRGQWGTVCDDGWDIRDASVVCRELGCGEPVDALSDAYFGPGSGPIWMNDVDCSGLESTVKNCKSPGWSRNDCVHSNDSGVICSAFNFRHACSHGLVIKLIFLVSGKKLNCHFYKIIIFQQHYIRSQATW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DX05</accession>
    <name>A0A3B4DX05_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DENND1B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037401196.1">
        <property type="nucleotide sequence ID" value="XM_037545299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DX05"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028018"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001041.2">
        <property type="protein sequence ID" value="ENSPNAP00000028018.2"/>
        <property type="gene ID" value="ENSPNAG00000013596.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430140"/>
    <dbReference type="GeneTree" id="ENSGT00940000155446"/>
    <dbReference type="OMA" id="TMESPHE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.200:FF:000003">
        <property type="entry name" value="DENN domain containing 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000001">
        <property type="entry name" value="Putative DENN domain-containing protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040032">
        <property type="entry name" value="DENND1A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196">
        <property type="entry name" value="DENN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196:SF24">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="14"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="434"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="570"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="573"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028018.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="781" mass="87079" checksum="F23AC4B2A820B569" modified="2024-11-27" version="2">MDSRIRENPERTFDLVLQVACPASENEDPTVLWKFPQDFGDQEVLQTVPKFCFPFDVERVSQNQVGQNFTFVLTDIDSKQRFGFCRLTSGCKVCICLLSYLPWFEIYYKLLNTLADYLTKRQENDLSDMLETLHALAVPKPNTPVNLSVHSYFIAPDINGLPTIPESRNLTEYFVAVDVNNMLQLYASMLHERRIIITSSKLSTLTACVHGSAAMLYPMHWQHIYIPVLPPHLLDYCCAPMPYLIGVHLSLLERVKSRSLEDVVILNVDTNTLESPIDDLHNLPSDVVSNLKSKLKKQSTATGSGVARAFLRSQAALFGSYRDALRYKPGEPITFCEESFVAHRSSTMRNFLEMAVNLQLFKQFIDGRLAKLNAGRGFTDVFEEEITEGGFCGSNSRSYQQWMHTVKRGVNKASPAVKSVYKIAKVHAKKGLQGVKDRIRQKDTEDEDGPYCAGSPTSTKSLSPRRLQKRRYQVQKSPVIVGFNDYVIDGEDEQDTASKISSEDSGEVPSYTEDSDESELEPEAGRAGQMNLLEEILDSLSTTSIEQGKLSAAKSLDFFRSLEDLDCDFTNKSRSPSGSSPAEAGSVDGVGSGDQSGWNMGQDDSALHGKHLPPSPRRQCSTRSLPVPLPKASKDLPDTGLPQPSARNSFQEEPFQPEKGKPIIADAPAHLEPLSDQQLSPDDQQPVASCALRRKVLSKQTLRQYQERQGGAGATGQVGRQPSMLVSWEKEREDHRGQIEEEGVQEKGLLEEIESMCLLRTADDSSLQLKQIHNNTDLNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXB8</accession>
    <name>A0A3B4DXB8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transmembrane protein 100</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TMEM100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXB8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027701"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001877.2">
        <property type="protein sequence ID" value="ENSPNAP00000027701.2"/>
        <property type="gene ID" value="ENSPNAG00000013248.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154322"/>
    <dbReference type="OMA" id="HALECKL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071773">
        <property type="term" value="P:cellular response to BMP stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032536">
        <property type="entry name" value="TMEM100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16311">
        <property type="entry name" value="TMEM100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027701.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="131" mass="13928" checksum="EFA26C6F487C3FC6" modified="2024-11-27" version="2">MSTHCAVKTNSIFINELELTVATGGTGGSCYRCTLPFGVVLLMIGVAVTAVAYSFNSHGSTISILGLVLLSSSVLLLGLTAVCWKVRRGKEMEMRSRSQTTLVASETAGTGMEEAFFCHLKHTHALECKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DY54</accession>
    <name>A0A3B4DY54_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PIP5KL1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017557133.1">
        <property type="nucleotide sequence ID" value="XM_017701644.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DY54"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001175.2">
        <property type="protein sequence ID" value="ENSPNAP00000027991.2"/>
        <property type="gene ID" value="ENSPNAG00000013549.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429701"/>
    <dbReference type="CTD" id="138429"/>
    <dbReference type="GeneTree" id="ENSGT00940000158633"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17304">
        <property type="entry name" value="PIPKc_PIP5KL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF46">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="3">
        <location>
            <begin position="29"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027991.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="448" mass="51690" checksum="492E19784CAF60B3" modified="2024-11-27" version="2">MNQAEMEMSGPGGSRRTRTVKRRVWGGLRQWKLLGMFEIDQQHEFYSLTCMMKEGLSAAVQNTIDNPIPDELSEDDYKLEVTQIHKDFRMETFAGPVFASLRRSLGMTEKEYQHSLSSEGCYLQFISNSKSKADFFLTNDKRFFLKTQNRREVRFLLSNLRIYMEHLEKFPHSLLVKFLGVHRIKIPHRRKKYFIVMQSVFYPDERIVARYDIKGCEVSRWTDPAPEGSQVIVVLKDLNFEGQFITLDHQRPWLLRQVEIDTAFLRALNVLDYSLLLAHQPLHQDERNHSLSFATLIMRTKKSVNAVSGPAVPGAVPEEDSTVLAPGPEVDCGTSQGHVTDIHGDGSRGHSPEQGERSSRAGAESAELREFQAQNRRLLPNFKNPLHVIDGPEQRYFIGIIDIFTVYGFKKRLEHLWKRLRHPGQSFSTVSPPVYCQRLCQWVNDHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E050</accession>
    <name>A0A3B4E050_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17 20">60S ribosomal protein L23a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Binds a specific region on the 26S rRNA. May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the large ribosomal subunit. Interacts with LYAR and GNL2. Interacts with MDM2; this interaction may promote MDM2-mediated p53/TP53 polyubiquitination. Directly interacts (via BIB domain) with IPO5, IPO7, KPNB1 and TNPO1; these interactions are involved in RPL23A nuclear import for the assembly of ribosomal subunits. Interacts with IPO8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017554117.1">
        <property type="nucleotide sequence ID" value="XM_017698628.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E050"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029055"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015804.2">
        <property type="protein sequence ID" value="ENSPNAP00000029055.1"/>
        <property type="gene ID" value="ENSPNAG00000014945.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427990"/>
    <dbReference type="CTD" id="6147"/>
    <dbReference type="GeneTree" id="ENSGT00950000182901"/>
    <dbReference type="OMA" id="RLDHHKV"/>
    <dbReference type="OrthoDB" id="1267328at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001015">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005633">
        <property type="entry name" value="Ribosomal_uL23_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03939">
        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0164">Citrullination</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 21" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="12 21" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="23">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="155" mass="17620" checksum="2725556E265ABE3E" modified="2018-12-05" version="1">MAPKAKKEAVPAKTEAKSKALKAKKAVLKGVHSQRKKKVRTSSAFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKHAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0W4</accession>
    <name>A0A3B4E0W4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Steroid 5-alpha reductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0W4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028889"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015468.2">
        <property type="protein sequence ID" value="ENSPNAP00000028889.2"/>
        <property type="gene ID" value="ENSPNAG00000014764.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182886"/>
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        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
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        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
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        <property type="entry name" value="TECR-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF31">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="TECR-like N-terminal" evidence="12">
        <location>
            <begin position="38"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="192"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Pfam" id="PF21696"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028889.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="40010" checksum="930E15BA130768CC" modified="2024-11-27" version="2">MDVLALEAKGGKADASPAPAPAKSRSKVPKKAKRIVYFEVEILDAKNKEKLLLLDKVLCLAVCLCYILFHVLSVSPDQRWYPARQSLCLDPKGKPLKDEDVLHQLPVGTTATFYFRDLGSQISWRTVFLTECIGPLFIYLMFYFRVPFIYAPKYDFTTSKHWVVHLACLCHSFHYVKRILETLFVHRFCQGTMPLRNIFKNCLYYWCFAVWMAYYINHPLYTPPYYGEQQVKTALGIFVFCQLGSFSIHITLRNLKPPGSKTKKIPYPTKNPFTWIFALVSCPNYTYEVGSWLGFTVMTQCVPVAFFTIVGFIQMTMWAKGKHRSYLREFKEYPTLRSPILPFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E111</accession>
    <name>A0A3B4E111_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4524 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017541720.1">
        <property type="nucleotide sequence ID" value="XM_017686231.2"/>
    </dbReference>
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        <property type="gene ID" value="ENSPNAG00000015446.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00500000044987"/>
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    <dbReference type="OrthoDB" id="75908at2759"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="entry name" value="C5orf34-like"/>
    </dbReference>
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        <property type="entry name" value="C5orf34-like_2nd"/>
    </dbReference>
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        <property type="entry name" value="C5orf34-like_C"/>
    </dbReference>
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        <property type="entry name" value="C5orf34-like_dom"/>
    </dbReference>
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        <property type="entry name" value="C5orf34-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34531:SF1">
        <property type="entry name" value="CHROMOSOME 5 OPEN READING FRAME 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34531">
        <property type="entry name" value="ZGC:153352"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C5orf34-like_N"/>
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    </dbReference>
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        <property type="entry name" value="C5orf34_C"/>
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        <property type="entry name" value="Polo_box_4"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <begin position="7"/>
            <end position="74"/>
        </location>
    </feature>
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            <begin position="118"/>
            <end position="214"/>
        </location>
    </feature>
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            <begin position="292"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="C5orf34-like C-terminal" evidence="2">
        <location>
            <begin position="405"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF15016"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="Pfam" id="PF22834"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029400.1"/>
        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="588" mass="65258" checksum="50A42ADAE97B6AA9" modified="2018-12-05" version="1">MAVVRFMVMFDDESVDVSFTDGSRLQLSPCGSEYVLETAPPAHPLHKRRRVRHRTRFTISEHRALLVDALNFRNKYAAHPYLPEELVPEDQKEKLGAEVSEVEWPSAGSCSISHSSAGDITVCSVDGHARLVLSSCGQEFTVEFMCRSSWNEERPQEPQDLRSAPAPSTAARHLRSSENEDAARSARRFTHTCVVQHHSGLHHHHTWRYPLSVALSQWEAQRAQCSDAIGQQRGAGVEGGLDHALTPVSQATVKCDLPRPLPLTCPCPHQHRWRYGSGAEDWPELDASAELVKVVWCHGVVYRLIGGAVPVVEVSLGDGSVIRSNGVLAEYFTHYRPSPSTAVETVYYLNGLPPDLPGQTYSIRSVVTRASRILQCYKQASTSTMSIMPQCCWRRQAEVKECAGVVLELQVAGTGQFQALSDGTAHITFLDGVQVQMLWNTHTPAQKQELFSGGVGPELCQLTLPDGQQHLLQVDVGGAYERYVAVAGQWRQWVKQNLLSASNYDRTEPQTDRPIDSRSVLTELQKIRRFNFLLDNSSILNSPSDPSQSFRLTSDHSDLTITNDSVSQALQKTTKAIQDINALLAQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2V0</accession>
    <name>A0A3B4E2V0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2V0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030013"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035893.2">
        <property type="protein sequence ID" value="ENSPNAP00000030013.2"/>
        <property type="gene ID" value="ENSPNAG00000016496.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="OMA" id="NIIWITQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000007">
        <property type="entry name" value="Vomeronasal 2, receptor 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043926925" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="12">
        <location>
            <begin position="23"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="548"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="582"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="615"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="659"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="704"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="771"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="550"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030013.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="820" mass="91830" checksum="D4D3D77F6F20237C" modified="2024-11-27" version="2">MDSIFTLLHVVMAIINFSSTNGNFCSLHGEPVYPPLSKDGNIIVGGIFPIHRTWEITDVSYKVKPSPMNCVRYFSQSLMFAVEEINNNSSLLPGVSLGYKIYDTCGSTTMGVRMAMALVNGNENSTLNEQCTKPAQVQAIIGDIQSSASMAIAKSVGPLSFPVVSLNAIFYHKTALAEMVKHFGWTWVGALRRDDDYGNSGMAGFTEAAEQLGICLEYSLPFFRTYSKEKVLRLIQQIKSSTSRVIVAYVTHWDFEILLDIFYEHNITGYQWVGTESWITDSAVARMDKHNILQGAIGLAIPKTTVTGLKEFILDINSLKSAGSAIFTELWETLFQCKYKVQNDSEGTTVCTGQEKLSDTENEFTDMTQMPIFSNVYKAVYAVAHTLHDLLGCTQICPTKKSSTSNFTALFLEHLKRVRFKTKEGEEVYFDKNGDPAAKYEIINLRTVEDHHEFVTVGLYDSSSPAHNRLLVKMASIVWAQNTNQMPVSVCTESCPPGTRKAVQKGKPICCFDCIPCAEGEISNMTGTNIHTHKKKRINGITVTHVRIFVINAKLFLIFVLIRFLVFLVHKNTPIVKANNSELSFLLLFSLALCFLCSLTFIGRPSEWSCVLRHTAFGITFVLCISCVLGKTIVVLMAFRATLPGSNVMKWFGPPQQRLSVVAFTLIQVLICVIWLTTSPPFPFKNLKHYKEKIILECQLGSAIGFWAVLGYIGLLALLCFILAFLARKLPDKFNEAKFITFSMLIFCAVWITFIPAYVSSPGKFTVAVEIFAILASSFGLLFCIFLPKCYIIIMKPEMNTKKGKYCLQHHYSHFIYYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3M8</accession>
    <name>A0A3B4E3M8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serotransferrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Transferrins are iron binding transport proteins which bind Fe(3+) ion in association with the binding of an anion, usually bicarbonate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transferrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574705.1">
        <property type="nucleotide sequence ID" value="XM_017719216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3M8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030298"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036064.2">
        <property type="protein sequence ID" value="ENSPNAP00000030298.1"/>
        <property type="gene ID" value="ENSPNAG00000017006.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440373"/>
    <dbReference type="CTD" id="100000381"/>
    <dbReference type="GeneTree" id="ENSGT00940000154388"/>
    <dbReference type="OMA" id="ESCNLGV"/>
    <dbReference type="OrthoDB" id="5914301at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13529">
        <property type="entry name" value="PBP2_transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016357">
        <property type="entry name" value="Transferrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001156">
        <property type="entry name" value="Transferrin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485:SF28">
        <property type="entry name" value="OVOTRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485">
        <property type="entry name" value="TRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00405">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002549">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00422">
        <property type="entry name" value="TRANSFERRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00094">
        <property type="entry name" value="TR_FER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51408">
        <property type="entry name" value="TRANSFERRIN_LIKE_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017432710" description="Serotransferrin" evidence="8">
        <location>
            <begin position="20"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="9">
        <location>
            <begin position="22"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="9">
        <location>
            <begin position="366"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="491"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="25"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="133"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="369"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="379"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="427"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="482"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="510"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="524"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="533"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="611"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="729" mass="80543" checksum="840F15D4BC89EC33" modified="2018-12-05" version="1">MKGACGIVIILLFSNVAWAKKMRWCTVSEAEQRKCADLAKALVAALPPVVITAFARLSCVRAYSTADCINKIRANKADLVTLDAGEVYSAVKQFGLTAVAKEIYTDGGCILAVAIVRHSSTLDIRSLQGARSCHSGARWTAGWSLPLGHLLSRNYLPWAEEQPLSQAVSAFFSVSCVPGAAAMAISLCSQCQGQKSYNPQRNAHCETSHSEPFYHNQGALRCLKYGAGDVAFLDHTALDSIDDSEKDEYRLLCTDGTRAPLSSFRKCNLGRGPGSGVVTRINNRKIARKFLTAAQMAFGWRGRERQRFQLFNSSVYGANDLLFSDVTERLSILQEDMDISQILGLDYVALLKGLGHEGSSLEDSVVRWCCISHAEQKKCEQWALSIKSDPLVCVRASSMSNCIEMIKKDEVDAVSLDATHTFIAGKCGLVPVVTEYYGEKCEFPQGEGHFETKDLPFLYGVAVVRRSSRNVYFGNMDGRRSCHGHMYSPAGWVLPAKHTLSAERNNSASCEPNKVYSEVFWKGCLPGGQGNLCKVCVGGTEEAATKRCSDNHNERYYGNMGALRCLVGDPSGKSYGDVAFMEHHNLEVNIEYLNSSGWDEGWLAWDFELLCEDGGRAAVTEWRTCNLGAIPPNTVMTRPVLIARIYDFLMKSQENTATRLDSEFHLFESQQYGESDLLFKDATQCLVHTSHMDYRTILGDEFSSQAESIFNCTDSDILKFCNQDVCSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E971</accession>
    <name>A0A3B4E971_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">PR domain zinc finger protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E971"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031806"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036960.2">
        <property type="protein sequence ID" value="ENSPNAP00000031806.2"/>
        <property type="gene ID" value="ENSPNAG00000019413.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286918"/>
    <dbReference type="OMA" id="WTIEKEV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19193">
        <property type="entry name" value="PR-SET_PRDM7_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003973">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000380">
        <property type="entry name" value="zinc finger protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044417">
        <property type="entry name" value="PRDM7_9_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF58">
        <property type="entry name" value="ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="17">
        <location>
            <begin position="77"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="609"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="637"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="693"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="721"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="749"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="777"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031806.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="804" mass="92974" checksum="339221CAEF1C8CDF" modified="2024-11-27" version="2">VPHSPNISYTEEEELKHEDNLCKETVQIASSSMTFYNCEECRSFFTDECEVHGPAVFIPDTPVPVGVADRARQTLPDCLEIRRCGITGSDLGVFNQRETISVGTHFGPYEGDLTDKEDAMNSRYSWVVSMGQCAEYIDAERETHANWMRYVNCAGNDEEQNLVAFQHKGGILYRCCRPIEPGQELLVWYAEEYARNHDIPFDYLWNRKCSVKETSDVLTQVFSCSLCPLSYTSKAFHHRHMKRCHQDEYLRLLKSGKIKYEDLVSSGPQQTPSRRLPKDPGEEGAHRCSDCGKSFTERTYLQLHQRTHTGEKPYGCSECGRRFSQSSHLKIHQRAHTGEKPYTCSECGKSYGQKGNLQLHLRRHRGEKPHRCSECGKSFIQPSSLLQHQRIHTGEKPFECSECGKTFRQRGHLQLHQRMHTGEKPYYCSECGKSFKRHSHFKLHHRIHTGEKPYHCSHCGKSFSDGMALKMHQRFHTGEKPYHCPECGKSYALLSSFLQHQRVHAAEKPYNCPECGKGFTQKSNLQLHLRIHTGEKPMAQTASAPCVLFPTQLPSGETSFRHEEYIRLLKSGEIERENMRFARGPGAPQTLLTNLPLRRTQEKTAKRSHQCSECGKSFTRQTHLHLHRRVHTGEKPYRCSECGRSFNDRSNLRTHKRIHTGEKPYQCSECGKTFTQLTHLQRHQLIHRGEKQFFCPECGRNFTQQIHLQRHLFTHTGENPFTCSECGKHFRDRGNLRTHQRIHTGEKPYACLECGKNFTQLVHLQQHQRIHTGEKPYFCSGCWRTFRHSSNLNTHKCSKTQSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E9A3</accession>
    <name>A0A3B4E9A3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E9A3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037302.2">
        <property type="protein sequence ID" value="ENSPNAP00000032390.1"/>
        <property type="gene ID" value="ENSPNAG00000020290.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154395"/>
    <dbReference type="OMA" id="RCEIHER"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="117"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41602" checksum="2642E9A5E58E09B1" modified="2018-12-05" version="1">MAAMDFDTLKDGIKCGICNDFFKEPTTIPCGHNFCMKCISNQWDNELYQFYNCPKCSKTFMTRPELVMNETLCVIGNKLQRLKLQDGAQYGVVAELKKKNQKPQPMTRDKMKAKEKLKKMMCAQHDRLLNFYCRDDKQCICEQCTLSEHMGHNTVTAVEERKEREQKVEECLQKARCEIHERELILQKISKTSDQIKDSSNCAVDASRSVFSELTVTLEKMKEELNKIIQEQETALLSQAQLEEKQQKLVIWSLQAMETELEALSHVKDDIPFIMKSQLVVSQQEADPVTIHVNENLSFEFVTVALNKVKDMLEDGCKVAIENIQQRVRNVTLVKTSVQNQKHPDRQVWRKFSNVSGPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EDU0</accession>
    <name>A0A3B4EDU0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PDE3A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EDU0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024365.2">
        <property type="protein sequence ID" value="ENSPNAP00000033459.2"/>
        <property type="gene ID" value="ENSPNAG00000022213.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156628"/>
    <dbReference type="OrthoDB" id="546632at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="6">
        <location>
            <begin position="642"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="362"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="488"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="559"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1030"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1116"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="530"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="559"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1030"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1128"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1148"/>
            <end position="1172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033459.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1204" mass="131628" checksum="CB2D6DF3A3AF7B68" modified="2024-11-27" version="2">MAVDCDSDRRAAAAAEHRSPRASERNGYVSTCVTPLERDCRCACVQGCGGLASAACVACVAVFLALLLRLVDWDAQRGGGGDCGAWTAASPSAPQPTDGGALCTLISPAFTLLCAFFWLGLYLIRGGALAGSVALLLAACHVGEAAAQWLVRGSDEQLLSFTASAAVLACVAGAALMVVRLGQGVSVIVFVSVVRALALISLQKVRAGWRPYVAYLLGVLGVLLARYADRLLPNQGAQRREGSASVTGARGDIPVFKRRRRSSSVVSSDMAHHAQSSSKSHRRTSLPCIQRDQSSGTSVMVDIAVMGEAHGLISDLLADPSLPPNTCSSLRAVSNLLSTQLTFQPLHHRPRLSPLLAFSDGHTCSDSEETPEKSERLAIPKRLRRSLPPGLLRRISSSWTTTTSATGLPTIEPGPVRRDHSASIKPVHDSPSSSMVNDSRNSSVLLAVSKSRSMSASFATSGPNYLYSKGRRGYPPFSISPMVSPCHSPPVQGTPVNSPTTKAPSVDLPDPGALQGNCPSPQTPHRALIHSQSAPTSSTTHWIPPPLCSSCGRPYSKMWHGEGSTDKGDKPQPSDDPVLASSDYDSTYETNHSDSSDFAQNEEDGEAQRKPFDTQDSCGTFQAEALVLHPLIPPEDKPILAPEPLVMEGLEGLMSQINNWNFPIFNLVEKTHGKCGCILSQVSYRLFEDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQAVPGLPTLGHNSGSVSESDSVSGITHGHMGYVFSETYPQMEDGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRSEYNFLANLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVTTYLLTTDTYPKENIKLKGIHIILIQVGHEGCSGIDWSNENDRLLVCQMCIKLADINGPLKHKDLHLQWTEGIVNEFYEQGDEEASLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDTAALMPGQWVEHLPDEAEEETGVEETDEEDEDTTEEDASTSSEQSQKLIPKKRRKVFCQIMQHLLENHDMWKKVIAEEAQSQTEETESSCLNNASEPILAIHEEEEETGSKEELTEDKEEETEGEEEMGPTEEEQIKEQTKETDETIEHETLVRKDQDTVNQDTETVEKVGEALQEWDETSDEQSKGDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EDV8</accession>
    <name>A0A3B4EDV8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Iron-sulfur cluster transfer protein NUBPL</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nucleotide-binding protein-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NUBPL</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Iron-sulfur cluster transfer protein involved in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). May deliver one or more Fe-S clusters to complex I subunits.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568924.1">
        <property type="nucleotide sequence ID" value="XM_017713435.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EDV8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033479"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024363.2">
        <property type="protein sequence ID" value="ENSPNAP00000033479.1"/>
        <property type="gene ID" value="ENSPNAG00000022134.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436753"/>
    <dbReference type="CTD" id="80224"/>
    <dbReference type="GeneTree" id="ENSGT00950000183193"/>
    <dbReference type="OMA" id="CNHESHI"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000709">
        <property type="entry name" value="Iron-sulfur protein NUBPL isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="325" mass="35216" checksum="2DAC653509279A46" modified="2018-12-05" version="1">MSLYHSRKFAQLCKLASAKAAADLRSSNRNAGPLAVVCKHVRFKSSGERALQERQKAHMARGLPKQKPIAGVKEVIVVASGKGGVGKSTTAVNLALGLMANDQSKRVGLLDADVYGPSVPKLMNLKGNPELTDNNLMRPLMNFGMPCMSMGFLVEDTAPIVWRGLMVMSAIEKLLRQVDWGLLDYLVVDMPPGTGDVQLSITQNIPIAGAVIVSTPQDIALLDARRGAEMFRKVNVPVLGLVQNMSVFQCPQCNHQTHIFGSDGARELARTLEVELLGDIPLHLNIRETSDKGQPVVVSSPDSPEAEAYRRIASAVVCRLTEESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EF62</accession>
    <name>A0A3B4EF62_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heparan-alpha-glucosaminide N-acetyltransferase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HGSNAT</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037389002.1">
        <property type="nucleotide sequence ID" value="XM_037533105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EF62"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034373"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026129.2">
        <property type="protein sequence ID" value="ENSPNAP00000034373.2"/>
        <property type="gene ID" value="ENSPNAG00000023659.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411545"/>
    <dbReference type="GeneTree" id="ENSGT00390000001491"/>
    <dbReference type="OMA" id="CHQCLYQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012429">
        <property type="entry name" value="HGSNAT_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31061:SF37">
        <property type="entry name" value="HEPARAN-ALPHA-GLUCOSAMINIDE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31061">
        <property type="entry name" value="LD22376P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07786">
        <property type="entry name" value="HGSNAT_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="581"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="618"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Heparan-alpha-glucosaminide N-acetyltransferase catalytic" evidence="3">
        <location>
            <begin position="248"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034373.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="644" mass="72323" checksum="C15BF3A9987E7036" modified="2024-11-27" version="2">MRKGQIQTSGEEKREEGVAADDMAPKKHQGLIFIAVIVAFLALCVAPLTAELPSSRPRRKTAALKMDEAALYVINEVESEMLLSIMSDHCFQCFPQPVGRIPAGPGPGVPATLGFTVSTQHALILQLNSTPLNTELCRMHFHFGEHGNYSLWVKNLNDLSQVNCTMVTDSEPINSYLPILLAFLVFASLYVLAAIWNSVKGLDAVRNLLLRLGSTMEAERLINSELGSPSRVVESSAESIIPATAGRRLRSLDTFRGLALVVMVFVNYGGGRYWFFRHESWNGLTVADLVFPWFVFIMGTSISLSVNGALCRGAMRSHLLRKVCWRSLQLFLIGVFIINPNYCLGPLSWDSLRIPGVLQRLAFTYLVVAVLDVLVARVHQDSSLTDVWWYPAHDIFLNWPAWICVITMETLWLCLTFLLPVPGCPTGYLGPGGVGDFGQYPDCTGGAAGYIDRWLLGENHIYQTPSTRVVYQTTVPFDPEGVLGSINSVLMAFLGLQAGKVLLHYREQHRQIITRFLVWSFLLGIFSAVLTKCSRNQGYIPVNKNLWSLSYVTTLSCFAFVVLLVVYYTVDVKRWWSGAPFFYPGMNSILVYVGHEVFEDYFPFRWRMANSQSHSEHLAQNLLATSIWVLISYVLYRKKIFWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EG22</accession>
    <name>A0A3B4EG22_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ATP10B</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EG22"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038424.2">
        <property type="protein sequence ID" value="ENSPNAP00000034244.2"/>
        <property type="gene ID" value="ENSPNAG00000023541.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159531"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000018">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000130">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF79">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE VB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="315"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="990"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1055"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1084"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1112"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1157"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="35"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="22">
        <location>
            <begin position="941"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="580"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="589"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        <location>
            <position position="390"/>
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            <name>ATP</name>
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        <ligand>
            <name>ATP</name>
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        <ligand>
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        <location>
            <position position="622"/>
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            <name>ATP</name>
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        <location>
            <position position="688"/>
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            <name>ATP</name>
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        <location>
            <position position="717"/>
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            <name>ATP</name>
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            <name>ATP</name>
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            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00034036"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF16209"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="1341" mass="151629" checksum="5B3E0904C00155C9" modified="2024-11-27" version="2">MTWTSWCCWTKWRDSSESERKLVSNLPYEGLKPKNQPNRRYAGNAIKTTKYSFWSFIPMNLYEQFHRWANLYFVGIAVLNFVPAVNAFQPQVALIPICIILSLTAIKDAWEDFRRYQSDRQLNGMTCLIYSRSQRSFVERRWKDVRVGDFVRVLSNEIIPADILLLHTSDPNGVCHMETANLDGETNLKQRKVVPGFSSTNSSFEPLNFSISEKLSTTGFGIENLLLRGCTVRNTADASGIVVYAGHETKSMLNNNRLRYKRSKLEKKMNTEVLFCVLLLICMCLIVAVGHAIWLQTFSSVPIYFVPNSDGTFTSAVLSGVFMFLTMIILLQVMIPISLYLSIELVKMGQVFFITQDADLYDEEKDTRVQCRALNITEDLGQIQYIFSDKTGTLTENKMVFRRCTIMGTEFPHEENGEFRCRITAVDGGNVFSALTQSWFLFPAHFSGFFIINQPVSENHNVIYTEAVDFCVVAQETEVIPDHTLVQLMEEEEAKPYLDFFLAMAICNTVVVSTETARRQRVRACSHTDLAFTAFTRRGAGLRLQQADMSLTRNRGSLSEPIHMDPTDSPVYDVPTFSGSVQGPDEGVNSPSNCRATTGRVQAGSSSVAPEEVCYEAQSPDEAALVHAAKAYGFTLLERTPDHVTVRLPSGKVLEFKVLDVLSFDSIRRRMSIIVRHPRSKEIIMYTKGADSGIMERLDVAIKTQTDLDMYAKDGLRTLCFAKKVVSEQAYMAWQVKRQEAMSAISKKEELLMDTADDMENNLTLLGATGIEDRLQENVPETIQALREAGIQVWVLTGDKLETAVNIAYSCKLLDQRDLVFTFNMENKGACKTMLNGILQELLRNGSLESEASIGLVIDGRSLSMLLDDDLQMQFVQLCTRCRSVLCCRATPLQKSRVVTLLRRRLKVMTLAIGDGANDVNMIQAADVGVGISGQEGMQAVMASDFAISRFKHLKKLLLVHGHWCYTRLANMIIYFFYKNVAYVNLLFWYQFFCGFSGTAMIDYWFMIFFNLIFTSSPPIMFGIMDREVSAYMLLQLPELYKRGQHAGGYRRSTFWIAILDAFYQSLVCFFVPYWTYAGSDIDIFTLGIPMNTVSLFTIILHLAIEIKSWTVVHWVIMVGSVIVYFVVSLVFSALCVSCNPPSDPYWLVQEQVADPMFYLVCILTTVVALLPRYTLHALRGTLVPSDHLKARQKDQLPPHHRDRWLREYRSLKELCASFPSMSSSTVPQDTTQSPSTPTAPLAAFSRTFLETRCSTTLLQKVKVICTLICTRPCALLGEVRPFVWVMFHRQKCKSTVLCTKIKMYCVKRRVSGQLRRGKSRIAQSSLSSAPECASSGPLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJ10</accession>
    <name>A0A3B4EJ10_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Wings apart-like protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">WAPL cohesin release factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with the cohesin complex throughout the cell cycle; interacts with both chromatin-bound and soluble pools of the complex. Interacts with RAD21; the interaction is direct. Interacts with PDS5A; the interaction is direct, cohesin-dependent and competitive with CDCA5/SORORIN. Interacts (via FGF motifs) with PDS5B; the interaction is direct. Interacts with a SMC1 protein (SMC1A or SMC1B) and SMC3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WAPL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJ10"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035865"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029463.2">
        <property type="protein sequence ID" value="ENSPNAP00000035865.2"/>
        <property type="gene ID" value="ENSPNAG00000026291.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015768"/>
    <dbReference type="OMA" id="WVANDSD"/>
    <dbReference type="OrthoDB" id="78088at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000085">
        <property type="entry name" value="Wings apart-like protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039874">
        <property type="entry name" value="WAPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022771">
        <property type="entry name" value="WAPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012502">
        <property type="entry name" value="WAPL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100:SF13">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07814">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51271">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAPL" evidence="19">
        <location>
            <begin position="615"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="31"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="216"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="297"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="356"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="84"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035865.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1176" mass="129818" checksum="B42A1C434427BB97" modified="2024-11-27" version="2">MTSRFGKTYSRKAGDGSSKFDEVLSNKRATLSTKWGETTYKAQLGSKRPGLKADQADRTKRPKLVEDDGSEDPFGFDSDEESKPVTSRNVAQPKVSSVRPTSTDTSRASLTLEPNSRVSQPASVEAVAPSRTSSALSGDRSAHRVPEDPGRFFSSSTGGKTYNWSGENMDRHSYSWYKNASECDRRPLAQTTTLKSEKCEDKDSFDAWSAVVGLGARSTSPLPVSRDLPSSTWTSGGYGRSSHLHSKPPAETHIEEVGQGDSEVPVEPSESEDHSQSLLRASNCRTYCRSNKGKQLGGADGFGSAFDSSSAASLKSSSGDSAGKTAVRSRTRDYTVLHPSSVSVCNVTIQDSMDRGMEEFTASASAADPGEAGRLRKKVETETKPVSRFRPTQSKSKKDSKLELFGFDDGETLAEEGDPASSGRSNYKIKYFGFDDLSESDSDEEESSAERRRAKRKAMAAAAPEEVSIETSVDSPEPRSSQESQSSSTGQRLDILEAPEANSRTMSEAVKKPQVKPQEKAKESRKIFSGPKRSPTKAVYNARHWNQPEAEELPPPPPSASQATPAGSSGGSKESNLPKGDEGVFKTPPLPPKVIKSVTIPTEPYQDIVTALKCRKEHKELYTVVQHVKHFNDVVEFGENQEFTDDFEYLATGLKSGQPLNTRCLSIISLATRCALPSFRMHLRARGKVAQVFKTLSDAPQHTNLALCTASLMYILSRDRLNMDLDRASLELMIRLLELEQDKSTDSQLTAKEMNKVKEKIRKLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVENLSREDDKENLVASLWGAERCLRVLESVTVHNPENQAYLIAYKDSQLIVSSAMALRHCEEMIQRYSREVSMSSCGTPLPYCSNSSVGKAVEDCMRAVIGVLLNLTHDNEWGSTKTGEQEDLIITALNCVLKVPQYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVEMEMDDGEVWESMCVSNQEELNTEGSHSAIAALVKLFLQREHAAVLAETETDDFINDVPKTNLDQSGEWQETSSGEIQWVASENNPTDDQKKKEDDEEELDLNKALQHAGKHMEDSIVASYTALLLGCLCQGSPMNVSTVRENLPKGDFSIMTEMLKKFLNFMNLTCDIGTTGQKSISRVIDYLEHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJ52</accession>
    <name>A0A3B4EJ52_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567781.1">
        <property type="nucleotide sequence ID" value="XM_017712292.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJ52"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036467"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040064.2">
        <property type="protein sequence ID" value="ENSPNAP00000036467.1"/>
        <property type="gene ID" value="ENSPNAG00000004211.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436070"/>
    <dbReference type="CTD" id="406604"/>
    <dbReference type="GeneTree" id="ENSGT01100000263497"/>
    <dbReference type="OMA" id="RYCSEHA"/>
    <dbReference type="OrthoDB" id="6264244at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="11"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="76" mass="8680" checksum="DA3E0968B72C5AFB" modified="2018-12-05" version="1">MLLKMSSKMTRSDNITHARKTVQQLRVEACIERIKVSKASADLMRYCSEHAKYDPLLMGIPASENPFKDKKPCIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELA1</accession>
    <name>A0A3B4ELA1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transmembrane anterior posterior transformation 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547543.1">
        <property type="nucleotide sequence ID" value="XM_017692054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELA1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036558"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031272.2">
        <property type="protein sequence ID" value="ENSPNAP00000036558.1"/>
        <property type="gene ID" value="ENSPNAG00000007018.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424200"/>
    <dbReference type="CTD" id="558330"/>
    <dbReference type="GeneTree" id="ENSGT00390000010628"/>
    <dbReference type="OMA" id="KSMPQSQ"/>
    <dbReference type="OrthoDB" id="29023at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002089">
        <property type="term" value="P:lens morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045724">
        <property type="term" value="P:positive regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="481"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="501"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="515"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="66940" checksum="78D51CC9E2D6BA6A" modified="2018-12-05" version="1">MAESLNVGLSEEKDLENENRSRTTSSWSNAARIRKAVPVTDMAETLGFYDRPLDNEEQRESSEVEDTEEPSLLRFMCAELTRGYFLEHDEAKYTERRERVYTCMRIPKELEKLMMFGIFLCLDAFLYVFTLLPLRTLLAFLRLLTMPCCGHRAHVCPYCRRSSVSRFLQPAQVCDLLKGLIMVLCYFMMHYVDYAMMYHLIRGQSVIKLYIIYNMLEVADRLFSSFGQDILDALYWTATEPKSRKRAHIGVIPHFIMAVFYVFLHAILIMVQASTLNVAFNSHNKSLLTIMMSNNFVEIKGSVFKKFEKNNLFQMSNSDIKERFTNYILLLIVCLRNMEQFSWNSDHLWVLLPDVLMVVTSEVAVDVVKHAFITKFNDITADVYSEYKASLAFELVSSRQKNAYTDYSDSVARRMGFIPLPLAVLLIRVVMSSVKIQGALSSVCLLLFYLGMITLKVLNSIVLLGKSCHYVKEAKMEGKLFERPNTSAPKQTPTKPSSTTCPPPSGEPKMPQPSPFITKQPTPRASTSLPPEPPPVTKETSEAPPEIPPNPVEGSSETPQSPELKHRAPKKDLLEIDRFTICGNRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EM46</accession>
    <name>A0A3B4EM46_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017569575.1">
        <property type="nucleotide sequence ID" value="XM_017714086.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000037547"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040986.2">
        <property type="protein sequence ID" value="ENSPNAP00000037547.2"/>
        <property type="gene ID" value="ENSPNAG00000004574.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437165"/>
    <dbReference type="CTD" id="219285"/>
    <dbReference type="GeneTree" id="ENSGT00390000013973"/>
    <dbReference type="OMA" id="WFSGEAW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000126">
        <property type="entry name" value="Zmp:0000000735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16155">
        <property type="entry name" value="DED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16155:SF3">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 9-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="12"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037547.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1543" mass="177733" checksum="1A77EF7D9351A32A" modified="2024-11-27" version="2">MEELSNTPVEKWTESMVSTWLRSIGVKEQYVKKLHEEEVDGRILLEITEDFLKKETGMKSGYVHLIITKRNELINASREVQRQTDTTHEKTVQKRDDKNNDQKPASVKGGSTTSSGQKGEIKQDSVAVRTTKSDAKPRPFGKRGIDYTYVKHGVLQPESGVIDLITPCHEYKAFTIAAALDCQRVQAKLAKEIFKFATGCMNMRSNGTIHFGVMDGKDNSGYVHGEIIGFPIQNKDTYADALNLIDRCFSSANVELVQQCIRPPEFILVIDPNSTEEHYVVEVDIVPSISIVRNRVFPVLLPNFKEKKNKIEFEIETIYRRLGSRTQPVTDRNEFYHGVQSRDAQREEKEQEHLFTIPDLCQDLGRKLIMLVTGGKKIMEQDKWYILVTNKFSKEDLSNIDFLLNMKLFCVFDFDPGSKESGLCHEYVKHHAVNLHFMQNYKIPSDTSVREFEGQLHLFEQTSWIFCNGRSDFKGNEPPFDENTWSKTRRTFLKDCVSLICKDILPKGTFLVVFLLTSPVETPLLNTFYEFFTDMEGHEDIICISESEENFRKWRNFALGSCDIETVNTSSVVGMKMSHVNATLQRVQTTTTRAAKYLPVYIKGECSLETRDEEKMCSLEILGTNQCEDTNPELIELEKGSIEREFYLGGKVTWMNFWLAEKGFVGEVIQRDAYHDVTNLLKDSLKWSLSQMPVNFINIYHHPGSGGSTVARQVLWKNRKELRCAVVKPSFSVGVVSEHAVMLREYEEKDPQKCCSVLLLVEDCEKEYLEDLKNELEVAAYTKKIAHGTPCFILLSCMRSYTPEDMCKGSHLQNVSVTHRLSAEEKRHFARKRQMLERQFKPEFILTFVLMSEEFEDEIIAKYVKQFVRHLLQGIDHANAVTQLIRYVALLNTYIQNSFISQSHCEALLPLALTVPMDRFRQHAFEASLSEQAKLVFIHLRDERTHIKSVRIIHPLVAKEILHQLLGDKQQQSDLALDLLNNDVLFEHRFGREDYVTFLRALFMRRSRISKGYESDSFFSPLIEHVRKKENPEKAIKVLGEAYKRFNKDAFFAQQLARLNYSHERFEEAEQWADTAAKKQPNNSYILDTKGQVYRRWFTAKCKAMEKIKKTPENTADAIKTALKAIECFQACQKAAVADVESMNNSGFFGTVEVCCSLLKLISSLRIFSKKTNSGSECLKYLLTEYIPTEVEKPWEHFHFKLKKLHAIVRNALEWISEDLSYFQTDLNTDDETSESCEQTLRHPKRWLVNKSAVYGKFFSDASFSSASSLRQSNPDGLTPFTKRMMICHLGGGNITTVLSILDQKENDAEAVRFLEDIILLYPKNPMTAKMDQLDLVNFIASHIALSAVSIQSPKLAAFSDLQRLSHLFPQDQSKCLSSALFLCMLLFWPEEHDTDDEKENKYETVLSAIEFLQKTYWSKMKDIPHRRRRIYTHFFLRNGVGYEKFVHKSKIEDLSKLTSVSEKRLKWFRGELWKTTEIADVLKHVTGWTENGTVYLEGPKKKKFPVHAVYVHSVPYDNENVTFYLGFTFRGPVAYNIKPTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EMW3</accession>
    <name>A0A3B4EMW3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">WHAMM</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EMW3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036511"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040074.2">
        <property type="protein sequence ID" value="ENSPNAP00000036511.1"/>
        <property type="gene ID" value="ENSPNAG00000027396.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000046704"/>
    <dbReference type="OMA" id="IQFYEIQ"/>
    <dbReference type="OrthoDB" id="6284683at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031738">
        <property type="entry name" value="JMY/WHAMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031808">
        <property type="entry name" value="JMY/WHAMM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330">
        <property type="entry name" value="P300 TRANSCRIPTIONAL COFACTOR JMY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330:SF6">
        <property type="entry name" value="WASP HOMOLOG-ASSOCIATED PROTEIN WITH ACTIN, MEMBRANES AND MICROTUBULES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15871">
        <property type="entry name" value="JMY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15920">
        <property type="entry name" value="WHAMM-JMY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="9">
        <location>
            <begin position="696"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="484"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="534"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="737"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="150"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="372"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="614"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="655"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="745"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="764" mass="86646" checksum="0D3853BFA6B4B80B" modified="2018-12-05" version="1">MSGADSERVDSLEGWVAVRSDLFSGPEALRLDFLVQWSEVEGQFAVTCRSRSPRGAAVAGQRGWAALLSAADITQAHSTLTSTAQELQHCLPDLSAFSPARNLLWDLLFSSTSRPENQQQLDVDGVCRQLEKYFSVAVELCGSRIVLDTLFSLQEEEEEEEEEEQFYEDLQEFKKKAMEEQVQRAKDRVRRIVQGHSSASGLQQLMKVYEEEDDAYDDLVAVATQFYQHLLQPFRQMREISTLYRLEIQKCVQTEELGPKRVAELEQEAEEWNKRGQEAESSVQDITVCYFNDTASSLAAMLKQMENDRKRFGSAAWAVVTPRLEKLKLLSAKESLQHMRARELCVTRSKQDIRDKLEGVCGAVREVQVLEVQYYEAQLELYDCKLEILKNEEQLLLAQIHTIRRQIKELSEEVVYYDACEDPAELQCIQTSTAHSGNNPTHSHLHQQLQQLERKRAATSTHRAKIRNRKESCVKAWELQQGELQQKTTQHQHHHAVHLKREKRREEEEQQKEWVELEREKTLSRLRSFREKKLGQFVMKTPRSKPKHPDSATDDSSQPMSIISLAPPTHDVSQSPASRPRGRGRGQKWREIPVQILLPPGPGPAVPEQTLTPPLLPPPPPPPPPPPPPPPPPPLPLHAPPLPLSDREVAPSPKIKNTLQESTGSMDAVLASLQRGQVRLRKVTPNAPTVSTEANFRDNLLSAIRQGVTLKKAPLPVGPTPCRDSELEQSIKAAMMRMKRVSKETDDEDDDDEQRDAQSGDWDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ENR5</accession>
    <name>A0A3B4ENR5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574019.1">
        <property type="nucleotide sequence ID" value="XM_017718530.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ENR5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031939.2">
        <property type="protein sequence ID" value="ENSPNAP00000036821.1"/>
        <property type="gene ID" value="ENSPNAG00000027963.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439879"/>
    <dbReference type="GeneTree" id="ENSGT01100000263473"/>
    <dbReference type="OMA" id="VEYMWIG"/>
    <dbReference type="OrthoDB" id="6369810at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="22"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="128"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="332" mass="38361" checksum="BDCBDA3322D03893" modified="2018-12-05" version="1">MEQKIILLLLVSGLGLGVSSLFFSREYHYVNEKKTWLDAQSYCRVMYSDLATIDNMDEMRMLMSVIDPCYNGSVWIGLKRGDQPSWGWSMGDDLLPKYSNWTNDGAAQTFPCGRISLTWSAYNCSQKYIGVCYDANPTDGRQYVGLTTEKTWKEAQTLCRNKYTDLVSIHSLEEHKKVADLMKNYSVEYMWIGLFSDSWKWSDLNNSSFRYWADSKPGQTDNCVSMALNDFGRWYNEQCSQQHNFVCYRDLKQRRQIGRVTVSSNSNVNLNDAAVRSTILRQIEQKLNMGPSVKLSWRWKDSKVFELRKKIPSAGGGAQKNMKKRKRATDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKY8</accession>
    <name>A0AAR2IKY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sulfotransferase</fullName>
            <ecNumber evidence="15">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017561435.1">
        <property type="nucleotide sequence ID" value="XM_017705946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKY8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058547.1">
        <property type="protein sequence ID" value="ENSPNAP00000038376.1"/>
        <property type="gene ID" value="ENSPNAG00000035435.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108432232"/>
    <dbReference type="CTD" id="767708"/>
    <dbReference type="GeneTree" id="ENSGT00940000166888"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000194">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF31">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="16">
        <location>
            <begin position="99"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="12">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="108"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="314"/>
            <end position="318"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="351" mass="39829" checksum="B7F997B97DC36DA8" modified="2024-11-27" version="1">MEYSLLCHASHAASLPPVRHKVLVLCIMLSLWAYTLYCCIGYCSSVPSLAADPGEPTSPHALPTELLNGDGGDLDSSLSRVDEWEEGRTLNGSETKKLPQAIIIGVKKGGTRALLEFLRLHPDIRAVGAEPHFFDRNYEKGLEWYRDLMPKSLEGQITMEKTPSYFVTKEVPGRIYAMSKDTKLIVVVRDPVTRAISDYTQTRSKKPDIPSFESLTFKNTTAGLIDSTWSALQIGMYARHLDRWLRYFPINQMLFVSGERLISDPAGEMARVQDFLGLRRVVTEKYFHFNPAKGFPCLKKPESSSKPHCLGKTKGRTHPNIHPDVVQQLRDFYKPFNKKFYYMTGQDFGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IS15</accession>
    <name>A0AAR2IS15_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cellular retinoic acid binding protein 2, b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IS15"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058183.1">
        <property type="protein sequence ID" value="ENSPNAP00000040431.1"/>
        <property type="gene ID" value="ENSPNAG00000017056.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157619"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="44"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="158" mass="17301" checksum="0670A794CC6C0F88" modified="2024-11-27" version="1">MLSVIIQMGGLIKVRKPTTGKSLLIHLWINDPSFLPLGGLCSVGVNVFLRKIAVAAASKPSVEITQQGETLSIQTSTSVRTTHVSFTVGQSFNETTVDGRPCTSFPKWETDRKITCEQTLQKGEGPKTSWTRELTNDGELILTMSAGDVVCTRVYERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IS90</accession>
    <name>A0AAR2IS90_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ASPP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037390576.1">
        <property type="nucleotide sequence ID" value="XM_037534679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IS90"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069234.1">
        <property type="protein sequence ID" value="ENSPNAP00000040897.1"/>
        <property type="gene ID" value="ENSPNAG00000026755.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436597"/>
    <dbReference type="CTD" id="568138"/>
    <dbReference type="GeneTree" id="ENSGT00940000153463"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000008">
        <property type="entry name" value="Apoptosis-stimulating of p53 protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000030">
        <property type="entry name" value="Apoptosis-stimulating of p53 protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047163">
        <property type="entry name" value="ASPP1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048945">
        <property type="entry name" value="RASSF8/10_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131">
        <property type="entry name" value="APOPTOSIS-STIMULATING OF P53 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131:SF16">
        <property type="entry name" value="TUMOR PROTEIN P53 BINDING PROTEIN, 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21712">
        <property type="entry name" value="RASSF8-10_RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Ras-associating" evidence="15">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="860"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="893"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="959"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="366"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="578"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="463"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="657"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="713"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="724"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="737"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1030" mass="114089" checksum="11DAA9D854FA4231" modified="2024-11-27" version="1">MMPMFLTVYLSNNDKHYTEVPVTPETLCRDVVDLCKEPGETDCYLAETWRGSERVIGDSERIIEVLQRWGQQRAEVRFFLRHNQVPNHETGGLRGPDLLQKRNTLKSSGERHLENGIVPLHMDITLSELQEMASRQQQQINAQQELLANKEQRLRFLKLQEQQASEQEKLRLLRENAENQEAKLRRVRALKGQVEQKRLSNSKLVEEIEQMNGLFQQKQRELVMAVSRVEELSRQLEMLRQGKLDESGHSSAGELERLYKELQLRNKLNQEQSAKLQQQRESLSKRTQEVAAMDRRVAELRERLWKKKAALQQKENFPQPADGHSSQPPVGQSRVAAVGPYIQCPPPPLPPRQEVVIKPAYPDGTATLPKPLAKPTGFQAGKISKLSDWSSSAEVSSVGGSQRGHASTLPRMSSHGSTEKDPEADVKPPPLPLRKNQSSEDLLRDGQCVGKVPPPVPSKPAKQSNFTKPCFSTGTFPGKARAAPQLTPPSNHSQRAQLPPSHHGLPLPLPPKQDTLPAATVRPFSVEGSAGKGAATPPTLHKPQTLAASSIYSMYTQHSSLAKGYQGQSTLTRSQPRLYGKPVIPSTGGQHSHSQDSSYSERGATTEADQVDHCVSCPGEGPESERVPRPLSPTKLLPFLSNPHRLQSDADLEALRKRLHHAPRPLKKRSSITEPEGPAGPNIQKLLYQKTTLAAMETPDREGSGSAPGQEDEAGRVQKEDDSVPPPLPPRSPVPDESGRGEQHPLLEEDEQEGGGPIPSEPYTEEYPPYPPPPYPSTGDQDGPGEDSSSMKAPEVTGQVTLPPGKRTNLRKVGSERINHGMRVRFNPLALLLDASLEGEYDLVQRIIYEVEDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFATTYSDMQTAADKCEELEEGYAQCSQFLYGVQEKMGVMNRGVVYALWDYEAESADELSFTEGDCMTTLNRDDGQESEWWWARCGDNEGYIPRNLLGLYLRIKPRQRSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IT73</accession>
    <name>A0AAR2IT73_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein light chain Tctex-type 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein light chain Tctex-type family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IT73"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059500.1">
        <property type="protein sequence ID" value="ENSPNAP00000042918.1"/>
        <property type="gene ID" value="ENSPNAG00000003235.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154531"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.40:FF:000001">
        <property type="entry name" value="Dynein light chain Tctex-type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF19">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="108" mass="11897" checksum="169E5C307F75F152" modified="2024-11-27" version="1">QLLVTADEIHIGASLKGNSTKFTIGGNAYQHSRVNQWTNNVVEQCLSQLSKLGKPFKYIVTCIIMQKNGAGLQTACSCFWDNTTDGSCTVRWENKSMYCIVSVFGLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWL2</accession>
    <name>A0AAR2IWL2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex I-B14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase B14 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NDUFA6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Required for proper complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Mammalian complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWL2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059250.1">
        <property type="protein sequence ID" value="ENSPNAP00000044100.1"/>
        <property type="gene ID" value="ENSPNAG00000036046.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018898"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20266">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045299">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016488">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006643">
        <property type="entry name" value="NDUA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="14">
        <location>
            <begin position="42"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="140" mass="16563" checksum="C016D127FC0F24B5" modified="2024-11-27" version="1">MNRFKRADSFASHSSAMASSVARGAVKAVKPIFSRDVDEAKRRVRELYRAWYREVPNTVAMYQLDITVRQGRDKVREIFNKNKHVTDPRVIDMLVIKGKMELQETIHIWKQKTHVMRYFHETENPCPNDFLSKFYHGHEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J2L4</accession>
    <name>A0AAR2J2L4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Serine/threonine protein phosphatase that may dephosphorylate and activate lipins. Lipins are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at different levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. May antagonize BMP signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the Dullard family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J2L4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065321.1">
        <property type="protein sequence ID" value="ENSPNAP00000044552.1"/>
        <property type="gene ID" value="ENSPNAG00000012474.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240503"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000044">
        <property type="entry name" value="CTD nuclear envelope phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FCP1 homology" evidence="13">
        <location>
            <begin position="60"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="247" mass="28658" checksum="31057D50C2F7E87F" modified="2024-11-27" version="1">MRMLKTRQCLLGIRTFLGVASRIWGFILYILRKHLRTIIQYQTVRYDLLPLSPASRNRLNAVKRKILVLDLDETLIHSHHDGVLRPTVRPGTPPDFILKVVIDKHPVRFFVHKRPHVDFFLEVVSQWYELVVFTASMEIYGSAVADKLDNNRGILKRRYYRQHCTLDLGSYIKDLSVVHSDLSSIVILDNSPGAYRSHPDNAIPIKSWFSDPSDTALLNLLPMLDALRFTSDVRSVLSRNLHQHRLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JA00</accession>
    <name>A0AAR2JA00_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Solute carrier family 46 member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC46A3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JA00"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055548.1">
        <property type="protein sequence ID" value="ENSPNAP00000048853.1"/>
        <property type="gene ID" value="ENSPNAG00000026127.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183096"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF9">
        <property type="entry name" value="LYSOSOMAL PROTON-COUPLED STEROID CONJUGATE AND BILE ACID SYMPORTER SLC46A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="52214" checksum="9683152D22B4371D" modified="2024-11-27" version="1">ASIFFTFYDPVVGLHAFAMFMTYPLLQQYVYRRLWHQLSGYPYPSTIKSSQNSIFLYSEFQAVQTETSRFLLQSELIFLLPCLFSSLLLLSYSDYRGRKVAMVPPLVGDFLFTLSYFIVSWFSLSLGYLLAAAFLAGLLGGPNTLLGGCFSYVADRCQERPAGKKTVQMARLDAVLGVLSGLGSLCTGFFIRAAGFSWPFLTASMLHLLNLLYVLGAHFVAGRLQGVYLLFAASTRRRNTVLLLLLSAFIFYKVITKSGMSIFILFELNAPLCWNELLVGYGSALSTLIYLNSFAGVALLSRWLPDGHIALLGLLSIAGGLCMAAFAKTTPLMFLVRLPLLLSIMPAPVLRSMMSKLVPSSEQGAVFACVAFVEMLSMGLAFPVFSSIYAATISWFPGFSFLLAAGLALIPHISSAKLHCRAKNNLNFVLLMVSCSAVLWLRMDGEECSRLTTEDDTEGSTSSEILQLPQWHDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JG33</accession>
    <name>A0AAR2JG33_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JG33"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083807.1">
        <property type="protein sequence ID" value="ENSPNAP00000048811.1"/>
        <property type="gene ID" value="ENSPNAG00000017013.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154801"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23649">
        <property type="entry name" value="Khc_CBD_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01369">
        <property type="entry name" value="KISc_KHC_KIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000009">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059182">
        <property type="entry name" value="Khc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF68">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="8"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="919"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="341"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="424"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="85"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="987" mass="112411" checksum="C70A038408914A62" modified="2024-11-27" version="1">MADPAECTIKVMCRFRPLNNSEVERGDKYIPKFQGEDTVVIAGKPYMFDRVLQSVTTQEQVYNACAQKIVKDVLDGYNGTIFAYGQTSSGKTHTMEVRNEVWKERCEGNLHDTDAMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPEEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSSLGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSFNEAETKSTLMFGQRAKTIKNTVCVNVELTAEQWKKKYEREKEKNKVLRNTITWLENELNRWRNGETVPVEEQYDKEKANTDALVLDNVVNNDKPASTPGVPAVPGVRLTDAEREKCEAELAKLYKQLDDKDDEINQQSQLVEKLKQQMLDQEELLGSSRRDHDNLQAELTRLQLENEASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEFEALSEELNQKSTILMSIDSELQKLKEMTNHQKKRVTEMMSSLLKDLAEIGIAVGSNDVKQHEGSGMIDEEFTVARLYISKLKSEVKTMVKRCKQLESSQSESNKKMDENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEENVDLLNEELVKISAQEKVHALEKENEIHSTNEVKEAVEKQIQSHREAHQKQISSLRDELDSKEKLITELQDLNQKIMLEQERLRVEHEKLKSADQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEMDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENAARDRKRYQQEVDRIKEAVRAKNMARRGHSAQIAKPIRPGQPPVASPTHPNVVRGSGGGGLFQNSQPAAIRGGQQIKMQAKIMAVSILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JGL0</accession>
    <name>A0AAR2JGL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear receptor 2C2-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NR2C2AP</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NR2C2AP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JGL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081787.1">
        <property type="protein sequence ID" value="ENSPNAP00000051183.1"/>
        <property type="gene ID" value="ENSPNAG00000036623.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429545"/>
    <dbReference type="GeneTree" id="ENSGT00390000017748"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000070">
        <property type="entry name" value="Nuclear receptor 2C2-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="141" mass="16002" checksum="D4938211B7E48E7B" modified="2024-11-27" version="1">MAVSLICCNTQSRVSSVLNRDVKQFGKKFMFDSNEETCWNSDQGDQQWVLLEFPVLVKVSELRLQFQGGFSGKSCKLEGCSKEANLEHIVDFYPEDNNSLQSFPIQDAPLMQKLKIVFESSTDFFGRIIVYSLDVLGERAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JI63</accession>
    <name>A0AAR2JI63_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Extended synaptotagmin-like protein 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the extended synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JI63"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085203.1">
        <property type="protein sequence ID" value="ENSPNAP00000049551.1"/>
        <property type="gene ID" value="ENSPNAG00000005554.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156561"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008429">
        <property type="term" value="F:phosphatidylethanolamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08391">
        <property type="entry name" value="C2A_C2C_Synaptotagmin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04050">
        <property type="entry name" value="C2B_Synaptotagmin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04030">
        <property type="entry name" value="C2C_KIAA1228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000025">
        <property type="entry name" value="Extended synaptotagmin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000106">
        <property type="entry name" value="extended synaptotagmin-1 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037752">
        <property type="entry name" value="C2C_KIAA1228"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037733">
        <property type="entry name" value="Ext_Synaptotagmin_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037749">
        <property type="entry name" value="Ext_Synaptotagmin_C2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051634">
        <property type="entry name" value="Extended_Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039010">
        <property type="entry name" value="Synaptotagmin_SMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761:SF3">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-LIKE PROTEIN 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17047">
        <property type="entry name" value="SMP_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="17">
        <location>
            <begin position="103"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="280"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="424"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="852"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="969" mass="109269" checksum="8632048AB597D57C" modified="2024-11-27" version="1">MAVGEDAGSASAETPGRHAVSVLLAFGKCLGALLPVYLAGYFGLSLCFVVLGLVVYLGWRQHRDGKQARLNTAMYVLENERDFTTTRLFRSRRDLPAWVNFPDVEKVEWLNKILQQAWPFVGQYLEKLLVETIAPTIRTSSAHLQTFSFTKVDLGDKAVKVVGVKAHTEHDKRQVLLDLYISYAGNVEINVEIKKYFCKAGVKGIQLHGKLRVILEPLIGDVPLVGAVTMFFIRRPKLDINWTGLTNLLDIPCLNAMSDTMVMDAIASFLVLPNRLTVPLVADLHVAQLRSPLPRGIVRIHLVEAENLAAKDTFIKGVIAGKSDPYAVLRVGTQTFTSHHVDNNLNPQWREMYEVIVHEVPGQELEVEVFDKDPDQDDFLGRYYCSVHFKIITWFTLKDAPSGKVHLRLEWLSLLPSAERLNEVRQNLTVSSKTPDPPSAAILTVYLDQAQDLPMKKGNKDPSPMVQLSVQDTTKESRTVYGTNNPVWEDAFTFFIQDPRKQDLDIQVKDDDRSLTLGTLSIPLSRLLSSTELSMDQWFQLDNSGPASRIYMNAVLRGVLRIHLVEAQDLVAKDSFMGGMVKGKSDPYVKIRVGGLTFKSRVIKENLNPVWNELYEVCQEVQFDLYDKDMDQDDFLVILLISLCGIQILQYQSQHSYVNKAVPSAAVLFIYMEQAHGLPVSSILLHSDDGFLFLLQSVAQVLMHLKLYLIILFLQLANRSASPRWDEAFCFLIHDPREDIVSVKLSHGWGQPLGAVVLPVREILAEQDLVLDRWLTLDGALPESQILIRAELKVTGQPWPAVLSLCKSSRSKLDLFWVHVMYKTATQFTDTFVRACCACFCVCRGGVAGDSGRGQVKVTIAYSFVFLPLFCCRNLPPCSKDGSDPYVSFLLQPDKNRSTKRKTSIKKRDLNPEFNERFDFDLSLEEATQRRLDLSVKNSVSFMSRERELIGKLHLDLNQLDLKAGIPQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JPT4</accession>
    <name>A0AAR2JPT4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">VPS10 domain-containing receptor SorCS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SORCS2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle membrane</location>
            <topology evidence="4">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Postsynaptic density membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Recycling endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Synapse</location>
            <location evidence="23">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the VPS10-related sortilin family. SORCS subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JPT4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061485.1">
        <property type="protein sequence ID" value="ENSPNAP00000052204.1"/>
        <property type="gene ID" value="ENSPNAG00000026566.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234563"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000083">
        <property type="entry name" value="Sortilin-related VPS10 domain containing receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000003">
        <property type="entry name" value="Sortilin-related VPS10 domain containing receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.80:FF:000001">
        <property type="entry name" value="Sortilin-related VPS10 domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001399">
        <property type="entry name" value="Sortilin-related VPS10 domain-containing receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF9">
        <property type="entry name" value="VPS10 DOMAIN-CONTAINING RECEPTOR SORCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00801">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="22" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0967">Endosome</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="11 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="27">
        <location>
            <begin position="775"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00010818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00074088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1126" mass="126067" checksum="8084D4D8C9B01CBB" modified="2024-11-27" version="1">GPNPDKQPSPLSLLLQTLLLTLCLLVGNRAAVSPSALFFSLSLVFSPLSVARAMRLRALSPRSLLSLSLALALVLLAACAPPAASTSSSSAAPGSPSPGRLALSSSSFVLKGDATHNQAMVHWTGENSSVILILTKYYHADSTKVLESSLWRSGDYGTTYTKLNLMPGTTIIVTNFYICPSNKRKVILISSTINEREQMLFISTDEGASFQRQPLSFTLETLLFHPTEEDKLLAYSKEAKLYVSTDLGRKWTLLQERVTKDRVYWAVSGVDVDPDLVHMEMQDSSGGHLYVTCLIQNCSDKMVTAQFLGKIDHNSLSVQNDYIFVKVTTGNRTKHYISYRRNEFVQMRFPKYALPKDVQVVSTDENQVLLALQEWYQTDTYNLYESDPQGVYYSIILENVRSTKQPEENVLIDVLEVRGIKGVFLANQKEDGKVTTLITFNKGRDWEPLAPPTTDMNGKPVTCKQPDCHLHLHLRWADNPYVSGTVHTKDSAPGLIMGAGNLGSQLVEYKEEMYITSDSGRTWRQVFEEEHHILYLDHGGVIVAIKDTSIPLKILKFSVDEGHTWSVHNFTSTSVFVDGLLSEPGDETLVMTVFGHISYRSDWELVKVDFRQSFPRQCTEDDYDSWKLTNLQGEHCIMGQERTYRKRKESSLCIKGKSYTSALTAKPCQCTEKDFNCDYGFERAPSLKAEGDHCFPDFWHDPDAPPDNCHLGHTYESSTGYRKVVSNVCEGGTNKEQSAKQHSCPLLPPRGLQLGIKGQMLTVAPGDDITFVVHQEQGDTSSTRYQVDLWDGVRAIYENLTVTDEPIQHRYEKPGVYRVSVQAENSAGQDRATLYIQVTTPLQEVHLEVVPIAGRNQLVNLTAVVLPADANLTVFYWWIGDDLQPKLTLENSLLTRFPEEGEVSVTVQASNGRSTVQDSKTVRVYDRFQVIPLSFSPNLDRFNPNIPEWREDVGRVVTKILAKVTGVPQDSLVTMVKPGLPTTAQLYVLPPEGVPAKRSVFVDKRVSTIKQALRESNVSFIVRGGLRVLVTLADPDAGNEDSLTRPWLRFRKLPGRNVYAQMHNEKEQEMTSPVNHSDDTQNIIQGEDLIILHREVKVVLGVLPKDSYWYSVRCLPKQGTEPQSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JQQ2</accession>
    <name>A0AAR2JQQ2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JQQ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045682.1">
        <property type="protein sequence ID" value="ENSPNAP00000054348.1"/>
        <property type="gene ID" value="ENSPNAG00000033200.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000201395"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="98" mass="11178" checksum="D383BB2E418E568D" modified="2024-11-27" version="1">CTTFFGYEPLLQLISLQKASGCWEMDDALAEVFGRTEEEVVRQTPAEVEKGVWATVLALIWLHGFKMDAQVEWQFVAMKAVAWIHTQNGKLSILIRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JTG3</accession>
    <name>A0AAR2JTG3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Properdin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CFP</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JTG3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068626.1">
        <property type="protein sequence ID" value="ENSPNAP00000053557.1"/>
        <property type="gene ID" value="ENSPNAG00000003501.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442394"/>
    <dbReference type="GeneTree" id="ENSGT00940000161209"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049536">
        <property type="entry name" value="CFP_TSR-0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054019">
        <property type="entry name" value="CFP_TSR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052065">
        <property type="entry name" value="Compl_asym_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906:SF43">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22195">
        <property type="entry name" value="TSP1_CFP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18487">
        <property type="entry name" value="TSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043434241" description="Properdin" evidence="6">
        <location>
            <begin position="22"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="48780" checksum="37F6EF02E61CFB9C" modified="2024-11-27" version="1">MKTIMLCTILLLVLHIQPTVSQKVPCYAGFSLTDGTCSGILGEVTIDDCCLNSKYGYQENGVCKSCRTAQWSEWSPWSECSVSCMEGVQQRRRACYGIGRCYDPYRKGSIQTKPCVDRACCPENGGWSEWSSWFPCSVTCESGIKKRNRKCTEPAPKCGGSCLGDSEETARCDTGIVCPTHGSWSLWGSWGPCMGTCQNEGFPPPEQQRRRTCTSPPPSVVPRGNNCPGSETDSQPCTELPFCKVNGNWGNWGGLSPCSVTCGVGQHMQQRTCDNPAPKYGGQPCQGVDTRNILCNTNKYCPFDGHWSEWGQWTPCKSPNDRSITCKNRPGHRRRERDCLGRQYSGEYCPGEGVEHKTCYDIDNCEEVNVYWSEWSSWSLCKPDCGKNSIQRRKKECIPDISKYSDKNLEMFSGTPTKKCPPSDTEESRQCFNLPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JZA1</accession>
    <name>A0AAR2JZA1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PLAC8-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cornifelin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JZA1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061491.1">
        <property type="protein sequence ID" value="ENSPNAP00000057383.1"/>
        <property type="gene ID" value="ENSPNAG00000035932.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287175"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="14199" checksum="54FEAE8DCDE654A4" modified="2024-11-27" version="1">MMVVLSFNFFVDNPKLIDMSNPVTHQPAAGSYGTNVQTGEWSTGLCSCCSDLLICGLGFLCPIALGCYTANKYGENACLACVPGGMAAMRTHMRLTYGIQGTICNDALMTCCCGIFETCRMAREIRIRNGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K127</accession>
    <name>A0AAR2K127_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K127"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075286.1">
        <property type="protein sequence ID" value="ENSPNAP00000058008.1"/>
        <property type="gene ID" value="ENSPNAG00000031296.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286909"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="126"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="595" mass="66396" checksum="95F2E9253BBEE5FF" modified="2024-11-27" version="1">MKPMYTFDSSKFNNLFSVYKLICPLNCFPLTPSADLCSKFINFFNLKIDSIYSQFAFTRPILNHQSLSVQFSGSCLSNFSTVNESFVGELIMKSRATTCQLDPIPTQWVKSCLPAICPLITKVINASLTAGLVPPCLKLAAVTPVLKKPGLDPENFANFRPISNLPLLAKVLERTVASQLHEHLESNDLYEVFQSGFRRNHSTETTLLRVVNDLLLAADQGMLNVLILLDLTAAFDTVCHNLLLTRLETLIGISGTALSWFKSYLSDRQQFVSIGGYKSDLCALSRGVPQGSVLGPLLFILYMLPLGNIIRRHGLQFHCFADDVQIYVYSKPSHTCPPAVLSDCLLDVRAWLVENFLSLNGTKTEAILISSPATARKLSSLAFSIPGFVVSSSAQVRNLGVIFDTTLSFEPHIKNVCKTAFFHLKNISRIRPFLSSAAAEIITHAFVTSRLDYCNAVLHGLPTRLLNRLQYVQNSAARVLTHKRSWEHITPVLRDLHWLPVQFRVQYKILVTVFKSLHDLAPTYLGNLLQMYVPTRTLRSSYSHLLIVPSTKFRTLGDRAFGVAGPKLWNGLPLQLRTSSSLSCFKAGLKTYLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K3P0</accession>
    <name>A0AAR2K3P0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K3P0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062674.1">
        <property type="protein sequence ID" value="ENSPNAP00000057092.1"/>
        <property type="gene ID" value="ENSPNAG00000008888.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015236"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027886">
        <property type="entry name" value="SPMIP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31393">
        <property type="entry name" value="C5ORF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31393:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15093">
        <property type="entry name" value="SPMIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="464" mass="52710" checksum="7CB66E4D741AFD43" modified="2024-11-27" version="1">YYDLTPNKRSNLRINDELEKLIKVSIQREHPYQSHISRCAIFPTYRCPDDPHTGVRAATKLPLNPLLPASAPQVTLLNKTKGAPYRHELLDVSMATQRNVTTWPGQNGFQNVKMQVFYPKAPKTLCPNMTLRDWKTTLSDRTANMLSNLEKVQWLTSYQLHYTGKRERSYPTFLPSRPLEGRKARILQNSRPLESRYTSPSLSSPTESPGWGLVSTQTLGAPEAFAEKIPNQSRHYASGKSEVFKPNEDNESGQPRHTERFNSAVYACELCWERNCECRVKLSEPGSTEPQPTIKTEKTKLEPFFRQNIFQPALQDKEHQAGPGNLSNSKVLKHMAGRNPFELSKFAPSIVDKRDGLVALEQPLKQSVEEKQRVSGVSHASSCIQPRSSSAPFRHGGRTSSRDVENGLFGSRSALLELQDSFSQSEAHRHFHETLQSTSMDLRDNHHSGRKHSFYGFNSYYFHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K529</accession>
    <name>A0AAR2K529_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Solute carrier family 25 member 15b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037389039.1">
        <property type="nucleotide sequence ID" value="XM_037533142.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037389040.1">
        <property type="nucleotide sequence ID" value="XM_037533143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K529"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061849.1">
        <property type="protein sequence ID" value="ENSPNAP00000059418.1"/>
        <property type="gene ID" value="ENSPNAG00000034258.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411990"/>
    <dbReference type="CTD" id="565335"/>
    <dbReference type="GeneTree" id="ENSGT00730000110966"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000059">
        <property type="entry name" value="Mitochondrial ornithine transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000066">
        <property type="entry name" value="Mitochondrial ornithine transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF46">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 15B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="104"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="209"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="32784" checksum="8819D8AFF7E689E8" modified="2024-11-27" version="1">MAPHPVVQAIIDLSAGAVGGAACVFSGQPLDTAKVKMQTFPGLYRGFLHCFLSTYRQVGLRGLYQGTSPALMANIAENSVLFMSYGFCQEVVRFTCSLEKGAVLSDMQKACAGSVASVFSSLVLCPTELVKCRLQAMHEMAASGQIATAQNSVWSVVKSIAQNEGPTGFFQGLTTTIAREVPGYFCFFGAYELCRTFFADYLRCSKDDIGVAPIVFSGGFGGACLWLVVYPMDCVKSRIQVMSMTGKQAGFFKTFMHIFRTEGFRALYSGLTPTMIRTFPANGALFLGYEASRKLMMSQFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KA58</accession>
    <name>A0AAR2KA58_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CXCL14</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017545592.1">
        <property type="nucleotide sequence ID" value="XM_017690103.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KA58"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073571.1">
        <property type="protein sequence ID" value="ENSPNAP00000061203.1"/>
        <property type="gene ID" value="ENSPNAG00000033017.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108418091"/>
    <dbReference type="CTD" id="9547"/>
    <dbReference type="GeneTree" id="ENSGT00390000000618"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043322163" description="Chemokine interleukin-8-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="3">
        <location>
            <begin position="24"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="99" mass="11908" checksum="A3AA6AADC827CBEF" modified="2024-11-27" version="1">MNRCTAAVLLLVIAIYSLNTEAYKCRCTRKGPKIRYKDVQKLEIKPKHPFCQEKMIFVTMENVSRFKGQEYCLHPRLQSTKNLVKWFRIWKDKHRVYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KED2</accession>
    <name>A0AAR2KED2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KED2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064702.1">
        <property type="protein sequence ID" value="ENSPNAP00000062688.1"/>
        <property type="gene ID" value="ENSPNAG00000036028.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287676"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="204" mass="22481" checksum="E84DDE129F5C156E" modified="2024-11-27" version="1">MHEINIHSPSFRPVNIQSPSFRPVNIQSSSFRPVNIQSSSSRPVNIYSSISRPVNIQSSSSRPVNIQSSISRPVNIQSSSSRPVNIQSSSSRPVNIQSSSSRPVNIQSSSFRPVNIQSSSSRPVNIYSSSSRPVKIQSSSSRPVKIQSSSFRSVNIYSSSFRPINIQRSSSRPVNIHSSNWRTYRSSCFFETLRVGGVIGPLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KG01</accession>
    <name>A0AAR2KG01_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KG01"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047095.1">
        <property type="protein sequence ID" value="ENSPNAP00000061036.1"/>
        <property type="gene ID" value="ENSPNAG00000035918.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271870"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="163" mass="18910" checksum="F6DEFA04676B0F43" modified="2024-11-27" version="1">MRNKILWSDETKIELFGVNARRHVWRKPGTAHHEANTIPTVKHGGGSIMLWGCFSAAGTGRLVRIEGKMNAAMYRDILDKNLLQSALDLRLGRRFIFQQDNDPKHTAKISKQWLQDHSVNVLEWPSQSPDLNPIEHLWRDLKMAVHRRLPSNLMERTHTYQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KIA5</accession>
    <name>A0AAR2KIA5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LTB4R</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556412.1">
        <property type="nucleotide sequence ID" value="XM_017700923.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017556413.1">
        <property type="nucleotide sequence ID" value="XM_017700924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KIA5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079655.1">
        <property type="protein sequence ID" value="ENSPNAP00000061836.1"/>
        <property type="gene ID" value="ENSPNAG00000035051.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429276"/>
    <dbReference type="CTD" id="1241"/>
    <dbReference type="GeneTree" id="ENSGT00950000182966"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004974">
        <property type="term" value="F:leukotriene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000109">
        <property type="entry name" value="Leukotriene B4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003981">
        <property type="entry name" value="Leukotriene_B4_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01476">
        <property type="entry name" value="LTBRECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="45"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="375" mass="42228" checksum="962CA6F0ADCCCE62" modified="2024-11-27" version="1">MASLALNTPISEPPNSSITSTNAFTMSQQIGIAILVLAFMFGFPGNVFVVWSVLCRVQQRSVTCLLVLNLAAADAFVLLSAPFFLRLLAGGQGWEFGDVMCKTVHYLCCVNMYVSIYLICLMSLDRWLAVTRPFLSQRLRNKRTIYVAMLCIWVLAFLLALPMPFYRSVLVHNGMTHCLPYHFNKGHKVFQYLFETLMGFVVPFTFILACYTSVICRLRSAMFHGRVKGSLLILLIIITFAVFWLPYHLINVLQVIGELQNNQTLSNLVMVARPNVTAFAFLSSSVNPILYVFAGSSYIRRAGLSFMAKLFEGTHSDHSVLSRRGTSTDNSNFAKLTIKSLQNRERVRSKSCGEEENKKMEKSCVEELKTLTTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKQ3</accession>
    <name>A0AAR2KKQ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Arachidonate--CoA ligase</fullName>
            <ecNumber evidence="13">6.2.1.15</ecNumber>
            <ecNumber evidence="14">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Long-chain-fatty-acid--CoA ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(9Z)-octadecenoate(out) = (9Z)-octadecenoate(in)</text>
            <dbReference type="Rhea" id="RHEA:33655"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(9Z,12Z)-octadecadienoate(out) = (9Z,12Z)-octadecadienoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45264"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a fatty acid(in) = a fatty acid(out)</text>
            <dbReference type="Rhea" id="RHEA:38879"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hexadecanoate(out) = hexadecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45256"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.15" evidence="13"/>
    <dbReference type="EC" id="6.2.1.3" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKQ3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060021.1">
        <property type="protein sequence ID" value="ENSPNAP00000063039.1"/>
        <property type="gene ID" value="ENSPNAG00000015460.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159323"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090434">
        <property type="term" value="F:oleoyl-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001579">
        <property type="term" value="P:medium-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000002">
        <property type="entry name" value="Long-chain fatty acid transport protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000008">
        <property type="entry name" value="Long-chain fatty acid transport protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006134">
        <property type="entry name" value="PRK08279.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF7">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="23">
        <location>
            <begin position="83"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="648" mass="71953" checksum="FC815F6FC17303C2" modified="2024-11-27" version="1">RMHYAATASLSFGSLGLLRLFGASWPWSLAAGLGVYLGTGGWRYFYVAVRTAKRDLNGLYVLLRVKLWIWRYMRNGSTVPSIFAQTVKHHPNKPALVYEATGETWTFSQLDQLCNAVAHWALSQGWTSGDVVALFMESRPLQVALWLGLAKVGVEAALINFNLRRDSLLHCLGVSGARGLVFAVELADAVSEVSSSLAPSAILFYTGDLTPDQVDALNAKPLDSILASSPRHPPPYTQSKGFNDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGYYSFRMRPDDVIYDCLPLYHSAGNIMGVGQCVIHGLTVVVKKKFSASRFWEDCIRHNCTVVQYIGEICRYLLSQPVRPSERGHRVRLAVGNGLRPSVWEAFMERFGIKQIGEFYGATECNCSIANMDGKVGACGFNSRILPNVYPIRLVKVDEDTMELVRNKEGLCVPCRPGEPGLLVGKINQQDPLRRFDGYASQDATRKKIAHNVFKKNDSAYLSGDVLVMDELGYMYFRDRSGDTFRWKGENVSTTEVEGTLSSMLGQTDVAVYGVAVPGVEGKAGMAAIADIAGGFNCESFLREIQKALPPYARPVFLRISPHVDTTGTFKIQKTRLQREGYDPRITTDQIYILNSRAGRYELVNEEVYNALVDGRIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KMC0</accession>
    <name>A0AAR2KMC0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MOB kinase activator 3B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MOB3B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KMC0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062339.1">
        <property type="protein sequence ID" value="ENSPNAP00000063619.1"/>
        <property type="gene ID" value="ENSPNAG00000035139.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271863"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000001">
        <property type="entry name" value="MOB kinase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25497" checksum="B23CF879602D6295" modified="2024-11-27" version="1">MSIALKQVFNKDKTFRPKRKFEPGTQRFELHKKAQASLHSGVDLRAAVQLPPGEDLNDWVAVHVVDFFNRINLIYGTVCEYCTEKSCPVMSGGPRYEYRWQDEQKYKKPTALPAPQYMNLLMDWIEVQINNEDIFPTSIGIPFPKNFIQICKKILCRLFRVFVHVYIHHFDRIIVMGAEAHVNTCYKHFYYFCTELNLIDRKELEPLKEMTSRMCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KTU1</accession>
    <name>A0AAR2KTU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S100/CaBP-9k-type calcium binding subdomain domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KTU1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068880.1">
        <property type="protein sequence ID" value="ENSPNAP00000067748.1"/>
        <property type="gene ID" value="ENSPNAG00000031049.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161854"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="CDD" id="cd05022">
        <property type="entry name" value="S-100A13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S100/CaBP-9k-type calcium binding subdomain" evidence="1">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="124" mass="13488" checksum="04FF1EE5F92402B6" modified="2024-11-27" version="1">MKVRLWVNTARGAGMARLDLQVLQHSDLEKAINTLVTNFHSASADKGPSLKADEFKSLLSSQLPNLMKTGGSDAGFGEVLKQMGVKDGEGITFKNFWNLIQNLATSQYGLLSNEKVSKCSCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KU71</accession>
    <name>A0AAR2KU71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">AGT</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KU71"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069872.1">
        <property type="protein sequence ID" value="ENSPNAP00000067883.1"/>
        <property type="gene ID" value="ENSPNAG00000033984.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163630"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="182"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="482" mass="54096" checksum="B6945089AE02AC40" modified="2024-11-27" version="1">MLEIAELDRLNALSPSPALFKNRVALQTEYDLITTTEAGRLILHARSNYYENGDKPSRLLGHQLRRRAASRIIPKVMDSGGTPSSDSLSINQVFKAYYSDLDKSESLSDDSVMSAFFNELEFPTVDHALAQELESPLTLDEIRAAIKTMQTNKAPGPDGFPLEFFKKFEKKLGPLLLAVFEESLGVGSLPQSMTQASISLLLKKDKDPTSCASYRPLSLLNTDIKILAKALALRLEAVLPNIISSAQNGFIKGRQLFFNTRTLLNVIFTKGSTLTSEVLVSLDAEKAFDRVEWNYLFNVLYKFGFKDNFISWIKLLYSSPKASVCTNGVRSDFFPLFRGTRQGCPLSPLLFALAIEPLSIALNSLNSFRGISRFGSEIKLSLYADDLLLYVTDPESCFPSILALLNRFSSFSGYKLNLQKSECFPSSKLNNQSPIPVVEHFKYLGIEIYPSLNKTVQFNYNHILEQVSNDLKKWTHFKHVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KV08</accession>
    <name>A0AAR2KV08_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KV08"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074081.1">
        <property type="protein sequence ID" value="ENSPNAP00000066232.1"/>
        <property type="gene ID" value="ENSPNAG00000030281.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000277642"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="110" mass="12632" checksum="04160D08F7A2BF0E" modified="2024-11-27" version="1">TMIPPPPNFTLGTMQSDKYCSPGNRQTQTRPMDFQMEKPLYAVLESLSQWSLDPRQVTSPSKKKKEKWVFILGEQEVSSPFRTLPLVGRQPDIIIFPAFGRCSYPEQLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KXW3</accession>
    <name>A0AAR2KXW3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KXW3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065280.1">
        <property type="protein sequence ID" value="ENSPNAP00000069105.1"/>
        <property type="gene ID" value="ENSPNAG00000036129.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161131"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF9">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="414"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="468" mass="53226" checksum="02D52F16BBC07C70" modified="2024-11-27" version="1">MMPLRAVEKPGFLHLMKKAVPMYQVPSRTYFATSEIPRMYQDVRASVEEQLKEAVCYSATTDLWTSSSGGGEPFISFTVHYISSDWQLKCHCLETHFFPEDHTSENISEMFENMLQEWKLPKEDLCGITTDNATNMKKAFSDFPCVWLTCFGHNLNLAVNKVLKIQRVESAVRACRHLIQGFSRSWKKKRDLKKKQADLELPEHALIHDVITRWGSTFEMISRFLEQQQAVCAVLANERSTWHLMPKDSDLAILENVSQLLQTFTLSSLSPVLEHIRSEILVEQAEDNALTRQMKQVMREDLEVRYTERLERLLQVSCFVDARFKGSFSKNLDDTVEACVEEAVTIATAVSPCQKQPQEEREENEVGEGSSSTTTATKRKGKGLSALLQQISSSRQNKATSENKNIHEKVMSEVKTYMSLPTIGTDADPLAWWKMHAEGMPFLAKVARKYLCIPATSGTLVKECSFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L9U0</accession>
    <name>A0AAR2L9U0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="8">PARP</shortName>
            <ecNumber evidence="8">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PARP8</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L9U0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079768.1">
        <property type="protein sequence ID" value="ENSPNAP00000071459.1"/>
        <property type="gene ID" value="ENSPNAG00000025464.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183129"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01341">
        <property type="entry name" value="ADP_ribosyl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000007">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051838">
        <property type="entry name" value="ARTD_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21328">
        <property type="entry name" value="POLY ADP-RIBOSE POLYMERASE FAMILY, MEMBER PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="1 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PARP catalytic" evidence="10">
        <location>
            <begin position="628"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="452"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="865" mass="96950" checksum="BED86EC60FE3D0CB" modified="2024-11-27" version="1">HRASTSMGFYFMGMCSRQERIQKDVDIVIQRCKAEKDCLFSDFRFTDATFTADHVIHLKADAIVLYNNIRLPFSLADNTYVSNSENDDEVLVTRDPIPVIFHRIATEIRINNDATSCLSIKSKLQIDKNPCHYAIEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLAAVRDLYGHHAVSLREYGAIDDVDIDLHIDVSFLDEEIALAWDVIRTEPVIVRLHCSLTQYLNGPVPTVDVFQVSTKDRFGLGHQLKKIMQTFVTQQWKHHTKDKINCPHSKKQTDKKAKSPLHIFSTLRRSPSYPPPGCVKNKKKLKPEQDGCVSKSHRLLRRTCSSAVKPEVDGCASKSHKLLGHSMSGEPRLELPSVLKPPAHRLLPRAAVKPEDCGGGLRPHRLLPRSGSGEPRCDALKPHRLLSRSCSGSVRTEELDGLKHHHRLLSRSYSSSTKMGKGELPKEPAGESRRLSLTSGLIGILAPALSSTPPPNNGAKSIPVRDRGFLVQTMEYAEQRIPVLNEFCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQTLAFNPRKKDYDRVMRALDSLTSIREMTQAPYLEIKKQMDRIDPLAHPLLQWVISSNRSHIVKLPVTRQLKFMHTPHQFLLLSSPPAKESNFRAAKGLFGSTFAFHGSHIENWHSILRNGLVVASNTRLQLHGAIYGSGIYLSPLSSISFGYSGMNKKQQKVASKDETATNKTNIHLQSQKKGQQPQFLQSRNLKCIALCEVITSPDLHKHGDIWVVPNPDHVCTRFFFVYEDGQVGDTSINTQDPSIHREILRVIGNQTATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LC96</accession>
    <name>A0AAR2LC96_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037392688.1">
        <property type="nucleotide sequence ID" value="XM_037536791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LC96"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078043.1">
        <property type="protein sequence ID" value="ENSPNAP00000071996.1"/>
        <property type="gene ID" value="ENSPNAG00000035555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108432279"/>
    <dbReference type="CTD" id="100130827"/>
    <dbReference type="GeneTree" id="ENSGT00940000167905"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF34">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="343"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="376" mass="42644" checksum="FC4438B072AE357F" modified="2024-11-27" version="1">MSDPMQELDELCYLTSQSMTSMVTSEHFKVLKKLGEGSYGKVMLAVHRKRGTPMALKFFPRCSTTLLAFLREYNLSLTFCTHPSLTRALGIFFSTPSHYIFAQQAALYGDLYDVIVSEVGVTEEQAQSVMSQLSGAVSHLHSLGFVHRDIKPENVFLYDRNCRWVKLGDFGLVRARGAQVRPVWYNSPFCVPEVEQAKEGKKGSKGEEEEEEEEETWMSVEPSLDSWALGILIYCLLTGCFPWEESTSDDPGFCKFRDWFNYTKEKEDREGGQNGWSHSNKNDRRDGGKGAEGAVPSQFESLSSLALTLLRKLLNPLPWLRAGPDEILSYLGGAWLLETERAEKRREKEAQEEAKKIREGGRGVEEVKGRQGRRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LGR0</accession>
    <name>A0AAR2LGR0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">1-acylglycerol-3-phosphate O-acyltransferase ABHD5</fullName>
            <ecNumber evidence="5">2.3.1.51</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Abhydrolase domain-containing protein 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors. Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Regulates lipid droplet fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37455"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74938"/>
            <dbReference type="ChEBI" id="CHEBI:74941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:37456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = 1-(9Z)-octadecenoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37443"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74928"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:37444"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37131"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:37132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + hexadecanoyl-CoA = 1-(9Z)-octadecenoyl-2-hexadecanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37143"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74551"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:37144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + octadecanoyl-CoA = 1-(9Z-octadecenoyl)-2-octadecanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37147"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74552"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:37148"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-hexadecanoyl-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:33187"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:64839"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:33188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1-octadecanoyl-sn-glycero-3-phosphate + (9Z)-octadecenoyl-CoA = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37163"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74560"/>
            <dbReference type="ChEBI" id="CHEBI:74565"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:37164"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:19710"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>eicosanoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate = 1-(9Z)-octadecenoyl-2-eicosanoyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:37451"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:74937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:37452"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LGR0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047613.1">
        <property type="protein sequence ID" value="ENSPNAP00000073566.1"/>
        <property type="gene ID" value="ENSPNAG00000033272.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016277"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010891">
        <property type="term" value="P:negative regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010898">
        <property type="term" value="P:positive regulation of triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000019">
        <property type="entry name" value="1-acylglycerol-3-phosphate O-acyltransferase ABHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF34">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE ABHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="27">
        <location>
            <begin position="48"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="328" mass="36475" checksum="F08C79A1DD957ABC" modified="2024-11-27" version="1">MCCLVIYITKIVLLGHVPISNGNYIWTLAFNENPLSAQACSNEGLQVPLVLLHGFGGGLGLWVKNLTALAQTGRPVYALDLLGFGRSSRPVACVQAQEAEEQFVQSLEEWREVLGLEAMIMLGHNLGGYVSAAYALKYPDRVQQLVLVEPWGFTERPDEEDLQCRIPLWIKALGSAMNPFNPLALLRLAGPLLLQALRSDFKQKYSSVFKDDTVPDYLYHLNVQTPSGETAFKNMTIPYGWAQRPMMQRMEMIDPSIRISVICGSRSCIDGQSGQAIQEMRSNSQTDLVITGAGHYVFADQSEDFNRAVLKICSNARDGEKAEQDRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LI55</accession>
    <name>A0AAR2LI55_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VBP1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 6">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Binds to the C-terminal part of VHL.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565754.1">
        <property type="nucleotide sequence ID" value="XM_017710265.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LI55"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083372.1">
        <property type="protein sequence ID" value="ENSPNAP00000074434.1"/>
        <property type="gene ID" value="ENSPNAG00000031223.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108434850"/>
    <dbReference type="CTD" id="7411"/>
    <dbReference type="GeneTree" id="ENSGT00390000018904"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000001">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016396">
        <property type="entry name" value="Prefoldin_subunit_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="195" mass="22404" checksum="3D1E869D24CFE748" modified="2024-11-27" version="1">MATTIESSNAGAASKKKHLGIPEAIFVEDVDSFMKQPGNDTADAVLRKLDEQYQKYKYMELNLAQKKLRLKSQIPQIKQTLEILRHMQKKKDTTDPMETHFLLADNVYCKASVPPTDKVCLWLGANVMLEYDIDEAQSLLEKNLATASRNLESLEEDLDFLRDQFTTTEVNMARVYNWDVKRRSKDNLLKSAERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LK09</accession>
    <name>A0AAR2LK09_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LK09"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048673.1">
        <property type="protein sequence ID" value="ENSPNAP00000076893.1"/>
        <property type="gene ID" value="ENSPNAG00000035448.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159113"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="4">
        <location>
            <begin position="63"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24943" checksum="357E23EE04FB20A0" modified="2024-11-27" version="1">MTFEKLAVACKWPKEDWAIHLIPLLTGKARSAFIAMDPGNNMDFEKLKEAILKKYEINADTYRSQFRAMDTSQDETPQELYIRIKDLFCKWVKYDTSSKDALMETMVLEQYMRVLYPEVRTWVKERNPMTAAEAATLVENFVAAHKGSKRYRYAGVLDRQPWGKSDGVGSGRGSGAPPNYSRNTPPPPQSKVPLQGVSCFNCGREGHKSPACPLRKPKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LL89</accession>
    <name>A0AAR2LL89_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cathepsin B</fullName>
            <ecNumber evidence="3">3.4.22.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.</text>
            <dbReference type="EC" id="3.4.22.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LL89"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046762.1">
        <property type="protein sequence ID" value="ENSPNAP00000075101.1"/>
        <property type="gene ID" value="ENSPNAG00000005563.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158680"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02620">
        <property type="entry name" value="Peptidase_C1A_CathepsinB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000031">
        <property type="entry name" value="Cathepsin B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012599">
        <property type="entry name" value="Propeptide_C1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08127">
        <property type="entry name" value="Propeptide_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043434267" description="Cathepsin B" evidence="11">
        <location>
            <begin position="24"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="12">
        <location>
            <begin position="84"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="353" mass="38662" checksum="6533CD427F04DFF9" modified="2024-11-27" version="1">MSWVVPLCVAFLCVLTALSMSSARPRLTPLSDEMVNYINKVNTTWRAGHNFHNVDYSYVKKLCGAKLNGYKLPVMDQYAGDIKLPKSFDPREQWPNCPTLKEIRDQGSCGSCWAFGAAEAISDRICIHNNGKVSVEISSEDLLTCCDSCGMGCNGGYPSAAWDFWTNEGLVTGGLYNSHIGCRPYSIEPCEHHVNGSRPPCTGEGGDTPSCEQKCEPGYGVSYKQDKRFGKKSYGVPSEEKQIMQELYKNGPVEGAFTVYEDFLLYKSGVYQHVSGSAVGGHAIKILGWGEENGTPYWLAANSWNTDWGDNGKTIISVLGESLKGIAHCLRSAMQHGSCTPQVSKSSLNKVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LMB2</accession>
    <name>A0AAR2LMB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">5-hydroxytryptamine receptor 3A-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Postsynaptic cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LMB2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075417.1">
        <property type="protein sequence ID" value="ENSPNAP00000075884.1"/>
        <property type="gene ID" value="ENSPNAG00000036618.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164924"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19063">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049944">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="46"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="167"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="42998" checksum="A7DF0CA1DF323A22" modified="2024-11-27" version="1">MDPHRAGAIWVVGHSQHCSNTDVVVVHSSAAGVFKHLSVWTESSPPTKNIHWDPSECGTERISLPRTSVWIPDIVINEFMDENKVPATFYLYVNHMGQVTDSLPFHVISSCNLDIYTFPFDTQNCSYTFNSYLHTSELKHIICVSDWTMSAGQIVLRRKAAVYVVNLLLPSCFLVALDVFSFLLPPQHVDRSAFKMTLILGYTVFLLLMNDLLPVTGNNIPLINVFLSVCLALMVGSLIETILVTNLLSGSAAYPRLPLWLRALLQRGARLVCLNKNSKAEENSRELSTLSVRTGDIGDTAPESGHPELLQELRNVSRDLVSIRLKVEDHLKTDEQTEEWIHLGLVIDRILFNFYIIFITMSFIMILVLWLSWYNKHNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LRB3</accession>
    <name>A0AAR2LRB3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RELA</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LRB3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053524.1">
        <property type="protein sequence ID" value="ENSPNAP00000077207.1"/>
        <property type="gene ID" value="ENSPNAG00000005984.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159867"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038061">
        <property type="term" value="P:non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01177">
        <property type="entry name" value="IPT_NFkappaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000003">
        <property type="entry name" value="NFkB p65 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000046">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR P65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHD" evidence="3">
        <location>
            <begin position="14"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="522"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="539"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="619" mass="67706" checksum="99A58B25488F162D" modified="2024-11-27" version="1">FLGIFGWGQPAMGQGNPCIEIIEQPKARGMRFRYKCEGRSAGSIPGEKSSDSTKTHPAIKVHNYSGPLRVRISLVTKSQPYKPHPHELVGKDCKHGYYEADLPERRVHSFQNLGIQCVKKKDVAEAISCRMQTQNNPFNIPEAKVWEEEYDLNAVRLCFQATITLPNGDLFPLEPVVSQPIYDNRAPNTAELKICRVNRNSGSCKGGDEIFLLCDKVQKEDIEVRFFRDSWESKGSFSQADVHRQVAIVFRTPPYCDTNLTEPVRVKMQLRRPSDREVSEPMDFQYLPSDPDEYRLVEKRKRTEGMLQNLKLGNIPPGEFYNLTPLRPFSTARRTFTASKPSAAASVSPVVNPLAASSVPAVKPFFSAVPGHLFAQPKVEQTSAPSTGDTWKFLNSLTVPSTTPAAPPTVSQDFHTVNLSDLHDFSFSSFPQSTLEQADNTARRDGLGSNVAGVSVAQEIQNNFSTQSAAPFREEPQPEDGDLPDFPSFSEAQTSDVDNINIEDFQALLGQSGLSGEGASGIGNSASASSTATPAGDTMASNGSNQVGNNMGSNATTWMNFPPSIVNLLQNEQMANAQPVSAASVGFDDIDVITSMDEDRLLSIFNSGSQVSFLSGHPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BM34</accession>
    <name>A0A3B4BM34_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM/homeobox protein Lhx9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May be involved in gonadal development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017558219.1">
        <property type="nucleotide sequence ID" value="XM_017702730.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BM34"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000618"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013543.2">
        <property type="protein sequence ID" value="ENSPNAP00000000618.1"/>
        <property type="gene ID" value="ENSPNAG00000000355.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430311"/>
    <dbReference type="CTD" id="791744"/>
    <dbReference type="GeneTree" id="ENSGT00940000158540"/>
    <dbReference type="OMA" id="CMTNERV"/>
    <dbReference type="OrthoDB" id="9990008at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021554">
        <property type="term" value="P:optic nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071632">
        <property type="term" value="P:optomotor response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031290">
        <property type="term" value="P:retinal ganglion cell axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021537">
        <property type="term" value="P:telencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021794">
        <property type="term" value="P:thalamus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09469">
        <property type="entry name" value="LIM1_Lhx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09377">
        <property type="entry name" value="LIM2_Lhx2_Lhx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000027">
        <property type="entry name" value="LIM/homeobox protein Lhx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000039">
        <property type="entry name" value="LIM/homeobox protein Lhx9 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000033">
        <property type="entry name" value="LIM/homeobox protein Lhx9 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF80">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN LHX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="79"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="141"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="281"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="283"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="346"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="424" mass="46290" checksum="08C7E90238D150B2" modified="2018-12-05" version="1">MNCAGPGLRLTEISARSSERSTCCSLSTMLFHGVPGADMRGVVEEMERRGKSDSAPISSAIDMGETETSAPSMSGERVALCAGCGGRISDRYYLLAVDKQWHMRCLKCCECKLNLESELTCFSKDGSIYCKEDYYRRFSVQRCARCHLGISASEMVMRARDLVYHLNCFTCTTCSKMLTTGDHFGMKDSLVYCRLHFETLVQSDYGAPFGHADVAHGKGAVPAGPLGLAYYNGVGTVQKGRPRKRKSPGPGADLAAYNAALSCNENDGDPMDRDSQYSSGQKTKRMRTSFKHHQLRTMKSYFAINHNPDAKDLKQLAQKTGLTKRVLQVWFQNARAKFRRNLLRQENTGVDKASDGSNLPGGTPSGPASEISNASMSPSSTPTTLTDLTNPTMPTVTSVLTSVPGSLDVHECRSPSQTTLTSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BND9</accession>
    <name>A0A3B4BND9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secreted frizzled-related protein (sFRP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538362.1">
        <property type="nucleotide sequence ID" value="XM_017682873.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BND9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001282"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012435.2">
        <property type="protein sequence ID" value="ENSPNAP00000001282.1"/>
        <property type="gene ID" value="ENSPNAG00000008075.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411352"/>
    <dbReference type="CTD" id="100538205"/>
    <dbReference type="GeneTree" id="ENSGT00940000156432"/>
    <dbReference type="OMA" id="VSKLCHR"/>
    <dbReference type="OrthoDB" id="5985572at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000001">
        <property type="entry name" value="secreted frizzled-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF149">
        <property type="entry name" value="SECRETED FRIZZLED-RELATED PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017473431" description="FZ domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="11">
        <location>
            <begin position="51"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="12">
        <location>
            <begin position="190"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="66"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="103"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="34633" checksum="EE0DE60BCD576772" modified="2018-12-05" version="1">MLARMAGRSVALSVLVLASSGLVFKADDDIKLDEVAASVISDFVTSAEFGITRSVCKPVPSTMGLCHGIGYEALRLPNLLGHDSVKEAQQQAAAWIPLVSKRCHSDTRKFLCSLFAPACLEELSAAVPPCRSLCAAVRDGCLPVMSAFGFPWPEAFNCSRFPAREELCIPGAEDAGRVEVTEKDRGIVLCDACSPASEGERDIQKNFCSSQFAFRLQMGSSTLEGVDLRVVPQGRSRILRWEGSEQEQQTAMQQSILWLPEATNCSCQALEGQVVGPLLALGNMQDGRMVLFRLMKWSRNEKELKKFIRKLTRQPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BS87</accession>
    <name>A0A3B4BS87_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Metalloendopeptidase</fullName>
            <ecNumber evidence="16">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BS87"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002508"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010321.2">
        <property type="protein sequence ID" value="ENSPNAP00000002508.2"/>
        <property type="gene ID" value="ENSPNAG00000008891.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183111"/>
    <dbReference type="OMA" id="FEMFRLR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000037">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000009">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF828">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15 16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 15 16" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 15 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="19">
        <location>
            <begin position="27"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="17">
        <location>
            <begin position="228"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="18">
        <location>
            <begin position="391"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002508.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="570" mass="65151" checksum="60EE007A9D262957" modified="2024-11-27" version="2">MNISLFWLTGAKTPLIEGDIVLPPGRNALINTAYRWTFPIPYILSDSLDLNAKGAIFQGFEVYRLKSCVDFKPYEGEKAYIKFEKRDGCFSSVGMQPSGQVLSLGPGCDHKAIVEHELLHALGFYHMQSRQDRDDYVKIWLDQVTDGLQHNFNKYDDSFVTDMNTAYDYESVMHYRPFAFNKDPNIPTITTNIPEFFNIIGQYLDFSEQDIVRLNKMYNCSSTLTLLDQCTFEYINICGMIQSSGDDGDWVHTKSTPGSEDHTVIGRCRDAGYTMHFDTAAGMAGESGMLESRLLYPKRKLQCLQFFYKMTGSSKDRLVIWVKMDDGTGTVRKPKKVHTIWADDDKTWKIVHVPMEVGVKFRYAFQAVKGDPDHSGGGIFIDDISLSETRCPSAVWRIQNFSQIMETANHDTFLDSPRFYSPEGYAFGIRVYPLSSHTDYTGNYTGLYFHLASGENDVVMQWPAVDRQATLVVMDQDPDIKLRMSSARSLTTDLATTTDGKLFWDNPTKVGTYDPTCQCYRSESRGWRNFIKHFDLRRRNYLKKDDLIIFADFEDLTPLKNSEVPVATEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUE8</accession>
    <name>A0A3B4BUE8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Transmembrane protein 87A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TMEM87A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LU7TM family. TMEM87 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUE8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009033.2">
        <property type="protein sequence ID" value="ENSPNAP00000003253.2"/>
        <property type="gene ID" value="ENSPNAG00000009672.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428628"/>
    <dbReference type="GeneTree" id="ENSGT00940000159481"/>
    <dbReference type="OMA" id="IFMRFLN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054101">
        <property type="entry name" value="TMEM87A/B_GOLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF81">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21901">
        <property type="entry name" value="TMEM87A-B_GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043646723" description="Transmembrane protein 87A" evidence="11">
        <location>
            <begin position="23"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="443"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM87A/B GOLD" evidence="13">
        <location>
            <begin position="21"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="12">
        <location>
            <begin position="224"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003253.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="64353" checksum="86B8555730F9D825" modified="2024-11-27" version="2">MTYASVFLSSLLLSSLCRSFGAEVSLWKIDVNLQRDGVFRKTLYPNSTIFMEFQGDLAACEEKKLAFNVSWYLRSSICYNEVFNTPDNTAKTMFGMHQKLKEDWTGFYSQGSIYFEDCASLFTQKVYYKEFQPVLPLSGQEEKPTNQTGNVWENKLEGAVAQSWSNSPYMFLVRFHPEVRPEASRGRRQTENSDVSSDSLEHFSLTMMVKVKGPHDYSSPADWPLMMFFMVMCIVYVLFGALWLLWLVCYWRDLLRIQFWIGAVIILGMLEKAVFYSEYQSIRYSGDYNHHAVIFGELLSALKRSLARILLLIVSLGYGIVRPRLGTTVPRLVAVGLLYLIFSSVEGVLRVTGGFSGTVALIANLSLSLIDSCVMWWVFISLSHTTRLLKLRRNVVKLSLYQHFTNTLIFSVLASIIFIIWTTKQFKFVDCQLAWREMWVDDAFWRLLFSTILLVIMVLLRPSVNSQRFSHSPLIDEDVDEDDDVKEPMLNEAFEGMKMRGAKSETHNSQKPLGKESSQDDDLKWVEDNVPTTVIDVALPAVLDDEEELLKTKMERSKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWH3</accession>
    <name>A0A3B4BWH3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWH3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009752.2">
        <property type="protein sequence ID" value="ENSPNAP00000002844.2"/>
        <property type="gene ID" value="ENSPNAG00000009211.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183145"/>
    <dbReference type="OMA" id="VYDEVRC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF27">
        <property type="entry name" value="SCAVENGER RECEPTOR CYSTEINE-RICH TYPE 1 PROTEIN M130-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="66"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="159"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002844.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="33950" checksum="4D6C74EBF0024046" modified="2024-11-27" version="2">MGCGRAANATHRAHFGQGSGLIFLDNVGCSGNECSLYNCSHNGFYKHNCNHSKDAGVICSGVLQSPTFNLTSSHSAVSSGEAVQFKCINLNSTCISANFSLYKNEQSIKTQTAESSAAFNLTVDSSHQGQYSCDYSYHREITSPRSSSINITVVNLLQPNITLSAPDGWFHWWLQGSEVIRGYNFSITCSIEPQYPGGSFHLEFSGTRNRTQSAVNHSNTFFFPKADFVDQGNYSCVYEVTVSSRTFSSPTTELLAVTVKVSPVPFIGSGVAAGLLLILVPIIIVLIKRCQKRKGQMSVKMDHSVCEYVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXD3</accession>
    <name>A0A3B4BXD3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 19">Polyprenal reductase</fullName>
            <ecNumber evidence="3 19">1.3.1.22</ecNumber>
            <ecNumber evidence="4 19">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>17beta-hydroxy-5alpha-androstan-3-one + NADP(+) = testosterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50820"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:50822"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54384"/>
            <dbReference type="ChEBI" id="CHEBI:13601"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:47909"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:54386"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18 19">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>androst-4-ene-3,17-dione + NADPH + H(+) = 5alpha-androstan-3,17-dione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:50816"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15994"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:50818"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 19">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.22" evidence="3 19"/>
    <dbReference type="EC" id="1.3.1.94" evidence="4 19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BXD3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003211"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009098.2">
        <property type="protein sequence ID" value="ENSPNAP00000003211.2"/>
        <property type="gene ID" value="ENSPNAG00000009614.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044920"/>
    <dbReference type="OMA" id="RFYETNF"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047751">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000021">
        <property type="entry name" value="Polyprenol reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0521">NADP</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="20">
        <location>
            <begin position="203"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003211.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="320" mass="36451" checksum="DCAF3B4365A835C7" modified="2024-11-27" version="2">MSQIFVFQRNSFTKAPHSVAREVVESRPGCCEDARRSSGDVEQVFQDLIRYGKTKRPLEQRRAVLRLLDVPKRWFWHFYAISVLWNGLLFVLSFRAVIFGEALPEFLTDILGFLTGCPPSAWTELPLTVLVLQALLWVHSFRRLLECLLVSIFSDGMIHVVQYAFGLSYYIFLGLTVLCAGVSPPTDGLLRDSTGPFLSQLTWYHIAGALLFIWASLLQHRSLSLLASLRTNSSGKVETLAHRIPHGGWFELVSCPHYLAELLIYTALGVCCGCCSLTWWLVVLYIFCNQALAAQLCHEYYQRTFKAYPRHRKAFIPLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5A1</accession>
    <name>A0A3B4C5A1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017561703.1">
        <property type="nucleotide sequence ID" value="XM_017706214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5A1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007052"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002486.2">
        <property type="protein sequence ID" value="ENSPNAP00000007052.1"/>
        <property type="gene ID" value="ENSPNAG00000012969.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432414"/>
    <dbReference type="CTD" id="568344"/>
    <dbReference type="GeneTree" id="ENSGT00940000163612"/>
    <dbReference type="OMA" id="SYMHVNT"/>
    <dbReference type="OrthoDB" id="253091at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF15">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="105"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="6">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="184" mass="20630" checksum="E85D0B06763B6BD5" modified="2018-12-05" version="1">MSGYGVSMEQLNDLLTDENGDFCMPSRDFNQVWPGILLGNESVALSVRRLQQLGVTHVLNAAEGDSLMHVNTGAEFYTGTDIIYHGIPACDTDHFNLSVYFEECSDFIEEALAYKGGRGKVYVHCQKGYSRSASLVIAYLMLRRRMDVRAAVATVRERREIGPNDGFLLQLCQLNDRLRDEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6K2</accession>
    <name>A0A3B4C6K2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FinTRIM family, member 82</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017557229.1">
        <property type="nucleotide sequence ID" value="XM_017701740.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C6K2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006740"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003058.2">
        <property type="protein sequence ID" value="ENSPNAP00000006740.1"/>
        <property type="gene ID" value="ENSPNAG00000012705.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429749"/>
    <dbReference type="CTD" id="338307"/>
    <dbReference type="GeneTree" id="ENSGT00940000166102"/>
    <dbReference type="OMA" id="GFIPKVT"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19802">
        <property type="entry name" value="Bbox1_TRIM8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16601">
        <property type="entry name" value="RING-HC_TRIM39_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001655">
        <property type="entry name" value="FinTRIM family, member 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF30">
        <property type="entry name" value="FINTRIM FAMILY, MEMBER 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="12"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="151"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="390"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="65424" checksum="BEE1E358301B4E75" modified="2018-12-05" version="1">MAEPMSPDYFSCHLCANLLRDPVAIPCGHSFCMDCISGYWNEADYTGIYICPQCRITFTQRPVLRPNATLTKVAEKIKKTGLNLNLPPAPPTNGSFAGPSDVPCDFCTVKKLKAVKSCLNCLASYCEKHLKPHYESATFKRHKLVDELGNLDRKICPQHQKSLELFCRTDQMCICAICTVSEHKGHDIVSAEAERSEKQKLLGVSQAEIKQKCQLRTKELEELKTAVDSLKSSAQRAMAESKKMFDEMITSIERMRSEVSKLININEKAAFSQAEGLMERLEQEIEELKKKETGLKQLYSTEDHIHFLQNFNYLCTPTDDGFIPKVSLNPDFSFSAARKAVAEIKERLEELGREELLRISKSVNEVPVYTLENRNVREKSSRVKEAHTVDSAPPSQPRSRSEFLKYFCQLRLDPSTAYKELYLSESNRKVTRTRNLQPYSDNPERFDSFAQVLCREALSGGRFYWEIEWSGEFSVGVAYRGISRKGKGSLCLLGYNDKSWSLLCSDSGYSAWHNRVDKAISAPHSPRIGVYLDHNAGLLAFYSIGETMTCLHRFETTFTEALYPGFGVGTSVKICQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEX6</accession>
    <name>A0A3B4CEX6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 84</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEX6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009855"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016317.2">
        <property type="protein sequence ID" value="ENSPNAP00000009855.1"/>
        <property type="gene ID" value="ENSPNAG00000015376.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007799"/>
    <dbReference type="OMA" id="MIQDEYT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028015">
        <property type="entry name" value="CCDC84-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31198:SF1">
        <property type="entry name" value="CENTROSOMAL AT-AC SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31198">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14968">
        <property type="entry name" value="CCDC84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="40240" checksum="88F617044D625239" modified="2018-12-05" version="1">MGPFYCEICRQTDFSGKGHIYGKSHQSKLKVVLVKFLEKVKEARRTIKHPQVEKFDATEHEKKFWCYCCGLEVQRHVTDGRFTVLHGALLEHMSTREHRANTHAFWWKNKADAKLKEKFIITEEEAERLKTEVAKALDQYEEKEDTLMKEQAAIIRYQEHHRLEVLQSLIEVCFPGMKQQYPLCTLHFEQFFHPHSYGHGEQAGPSHEDPFMAELGHGLTFIGYQVTPVDLLCRAVPPWLLKDPEDESAGSSKQEIGPSAQEFLKHRQQEKLKKLPPNRVGANFDHSSYTDANWLPSFGRVWNSGRRWQSRHQFRQEEAKSGQKRKRDHGRKATKKQKHLSNGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFE0</accession>
    <name>A0A3B4CFE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor-like protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017545994.1">
        <property type="nucleotide sequence ID" value="XM_017690505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFE0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009214"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015530.2">
        <property type="protein sequence ID" value="ENSPNAP00000009214.1"/>
        <property type="gene ID" value="ENSPNAG00000014755.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108421632"/>
    <dbReference type="CTD" id="550525"/>
    <dbReference type="GeneTree" id="ENSGT00940000155737"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04155">
        <property type="entry name" value="Arl3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000281">
        <property type="entry name" value="ADP-ribosylation factor-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="182" mass="20414" checksum="08DC03D3974F553F" modified="2018-12-05" version="1">MGLLSILRKLKSAPDQEVRILLLGLDNGGKTTLLKQLASEDISHITPTQGFNIKSVQSQGFKLNVWDIGGQRKIRPYWRNYFENTDVLIYVIDSADRKRFEETGQELAELLDEEKLSGVPVLIFANKQDLLTAAPASEIAEGLNLHTIRDRMWQIQSCSALTGEGVQDGMNWVCKNVTAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGF7</accession>
    <name>A0A3B4CGF7_PYGNA</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
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            <begin position="21"/>
            <end position="124"/>
        </location>
    </feature>
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            <begin position="71"/>
            <end position="98"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009961.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="124" mass="13557" checksum="4E71F446937B4D97" modified="2024-11-27" version="2">LFCTALPCTDLLCTALFCTALPCTDLLCTALFCTALPCTGLLCTALFCTALPCTDLLCTALFCTALPCTGLLCTALFCTVLPCTDLLCTLFCTTWLFILMRYFVLPYFVLHYFIFCVTLSCITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGU8</accession>
    <name>A0A3B4CGU8_PYGNA</name>
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        <recommendedName>
            <fullName evidence="9">Transmembrane protein 179B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMEM179B</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
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    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGU8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010560"/>
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        <property type="protein sequence ID" value="ENSPNAP00000010560.1"/>
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    </dbReference>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
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        <location>
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            <end position="129"/>
        </location>
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        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24290" checksum="A97392C89DE83A38" modified="2018-12-05" version="1">MVVMGLPWLLTLELVLHAGCFICGIIAAASLTFTQGHFAGQCILYGSVHYNTSSNSLILENSSSTSLCYFVSAISVCMAIYCISLILYWIYSSCVEDEVKRGSVLLNVSLGVCGLLLFFLLVSGCVLRIGRDSLCLSVLHNVPSISNCEEAQNKTWASPNTGNQFYTGLYSAERSVWVSFFFWVLILAVVVVQRRQAAGEDAEWSRAETEPFFHRPSRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIT0</accession>
    <name>A0A3B4CIT0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glial fibrillary acidic protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GFAP</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIT0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011275"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036063.2">
        <property type="protein sequence ID" value="ENSPNAP00000011275.1"/>
        <property type="gene ID" value="ENSPNAG00000016960.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427396"/>
    <dbReference type="CTD" id="2670"/>
    <dbReference type="GeneTree" id="ENSGT00940000159539"/>
    <dbReference type="OMA" id="QYETMAT"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045098">
        <property type="term" value="C:type III intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904714">
        <property type="term" value="P:regulation of chaperone-mediated autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006821">
        <property type="entry name" value="Intermed_filament_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF9">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04732">
        <property type="entry name" value="Filament_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="8">
        <location>
            <begin position="82"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="86"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="281"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="445" mass="51420" checksum="55EDE89A82414DA3" modified="2018-12-05" version="1">MESQRVLSSYRKRFGPQGSTGSPAVSSRMSTGRLSLHSTPRHLTLSSPGALSTSRLSLGGERLDFSADSMLKAQYRETRTNEKVEMMGLNDRFANYIEKVRFLEQQNKMLVAELNQLRGKEPSRLGDIYQEELRELRRQVDGLNAGKARMEIERDNLAADLATLKQRLQDEIALRQEADNNLNTFRQDVDEAALNRVQLERKIEALQDEINFLKKVHEEEMRELQEQLMAQQVHVDLDVSKPDLTAALREIRVQYEAMATSNMQETEEWYRSKFADLTDAANRNADALRQAKQEANDYRRQIQALTCDLESLRGTNESLERQLREMEERFTMETAAYQDTVSRLEDEIQTLKEEMARHLQEYQDLLNVKLALDVEIATYRKLLEGEESRITVPVQSFANLQFRETSMDTKLTPEAHVKRSIVVRTVETRDGEIIKESTTERKDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CP60</accession>
    <name>A0A3B4CP60_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CP60"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019578.2">
        <property type="protein sequence ID" value="ENSPNAP00000012329.2"/>
        <property type="gene ID" value="ENSPNAG00000018094.2"/>
    </dbReference>
    <dbReference type="OMA" id="SKPHAYQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029252">
        <property type="entry name" value="RGCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32193">
        <property type="entry name" value="REGULATOR OF CELL CYCLE RGCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32193:SF5">
        <property type="entry name" value="RGCC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15151">
        <property type="entry name" value="RGCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012329.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="105" mass="11819" checksum="50178272F562F20B" modified="2024-11-27" version="2">MTSPSIRLERRATASHDLLQEFNAVAEELSAPSLKAPHAYEHLLNEAKRRSNDGVNDSGIEDADEGESFLSHHLLIRPNVRFRKTKKGRRSLRGHILLGATSKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPH8</accession>
    <name>A0A3B4CPH8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 46">Amyloid-beta A4 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="53 54">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="53 54" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="53" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="53" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="44">Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G(O).</text>
    </comment>
    <comment type="function">
        <text evidence="46">Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 46">Cell membrane</location>
            <topology evidence="3 46">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell projection</location>
            <location evidence="10">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <location evidence="8">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 45 46">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="45">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550801.1">
        <property type="nucleotide sequence ID" value="XM_017695312.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPH8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013278"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020808.2">
        <property type="protein sequence ID" value="ENSPNAP00000013278.1"/>
        <property type="gene ID" value="ENSPNAG00000019164.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426075"/>
    <dbReference type="CTD" id="170846"/>
    <dbReference type="GeneTree" id="ENSGT00530000063252"/>
    <dbReference type="OrthoDB" id="6147836at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005798">
        <property type="term" value="C:Golgi-associated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030546">
        <property type="term" value="F:signaling receptor activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22607">
        <property type="entry name" value="Kunitz_ABPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.140:FF:000001">
        <property type="entry name" value="Amyloid beta (A4) protein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.230.10:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.230.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, amyloid-beta peptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013803">
        <property type="entry name" value="Amyloid_glyco_Abeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037071">
        <property type="entry name" value="Amyloid_glyco_Abeta_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF7">
        <property type="entry name" value="AMYLOID-BETA PRECURSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03494">
        <property type="entry name" value="Beta-APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00203">
        <property type="entry name" value="AMYLOIDA4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00204">
        <property type="entry name" value="BETAAMYLOID"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="36 46" id="KW-0034">Amyloid</keyword>
    <keyword evidence="23" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="30" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13 46" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="42" id="KW-0966">Cell projection</keyword>
    <keyword evidence="39" id="KW-0168">Coated pit</keyword>
    <keyword evidence="47" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="34" id="KW-0186">Copper</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="43" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="38 45" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="27" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="26" id="KW-0967">Endosome</keyword>
    <keyword evidence="40" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="35" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="33" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="37 46" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="41" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="54" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0964">Secreted</keyword>
    <keyword evidence="31" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="25 49" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0765">Sulfation</keyword>
    <keyword evidence="22 46" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="32 46" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="29" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="28" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="49">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017328272" description="Amyloid-beta A4 protein" evidence="49">
        <location>
            <begin position="19"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="46">
        <location>
            <begin position="686"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="51">
        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="50">
        <location>
            <begin position="303"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="52">
        <location>
            <begin position="368"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="45">
        <location>
            <begin position="29"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="45">
        <location>
            <begin position="132"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="48">
        <location>
            <begin position="218"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="48">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="47">
        <location>
            <begin position="393"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="48">
        <location>
            <begin position="233"/>
            <end position="271"/>
        </location>
    </feature>
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        <location>
            <begin position="74"/>
            <end position="118"/>
        </location>
    </feature>
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        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
    </feature>
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        <location>
            <begin position="134"/>
            <end position="188"/>
        </location>
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            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
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            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
    <sequence length="755" mass="85453" checksum="24F34435B4984C90" modified="2018-12-05" version="1">MGDRAALLLFIVATSSLAAEVPSDDSVGLLAEPQVAMFCGKLNMHINVQSGKWEPDPSGTKSCIGTKEGILQYCQEVYPDLQITNVVEANQPVTIQNWCKKGRRQCRSHVHIVVPYRCLVGEFVSDALLVPDKCKFLHQERMDMCESHLHWHTVAKESCGDQSMNLHDFGMLLPCGIDRFRGVEFVCCPVEDQRDSDAEEQEEAISDVWWGGAETEYSDRQAPLEQQEQEQATTEDDEDTDDDEEEAWDDEDGDGVEDVDEEDDEGEDATDERDTSEQASNVAMTTTTTTTTESIEEVVRAVCWAPARRGPCRAKLPRWYFVEEMGRCAPFTFGGCGGNRNNFESEEYCMAVCSSSVLPTMAPSPPDAVGRYLEAPGDINEHAHFQQAKERLEAKHRERMSEVMREWEEAERQAKSLPRADKKAVIQHFQEKVEALEQEAASERQQLVETHIARVEALLNDRRRLALDSYLTALQADQPRPRQVLSLFKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRPQMLTHLRVIEERLNESLSYLYKVPRVANEIQDQVVVLMQRVQAEATQQLSSLQTDMRVSYGNDALMPDLPDSTPPLDALPPEQDGLGFIHPERFNQANTDNHVEPVDARPAPDRGLPTRPVSGLKPEEMPEVRMDTEERHSAGYEVHHQKLVFFADDVGSNKGAIIGLMVGGVVIATVIVITLIMLRKKQYTSIHHGVIEVDAAVTPEERHLTKMQQNGYENPTYKFFEQMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQF7</accession>
    <name>A0A3B4CQF7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KIF18A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQF7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013006"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020474.2">
        <property type="protein sequence ID" value="ENSPNAP00000013006.2"/>
        <property type="gene ID" value="ENSPNAG00000021697.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169309"/>
    <dbReference type="OMA" id="QPMMSAV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000027">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF65">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="8"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="644"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="655"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="671"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="110"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013006.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="907" mass="101509" checksum="65681340FD5FE9C7" modified="2024-11-27" version="2">MSEEVCSHVKVVVRVRPLNAKETDGNYKKVVHVVDNHMLIFDPKEEEVTFFRGQRVGNRDVRKRANKDLKFVFDNVFGENSSQGDVFENTTKGVVDGVLNGYNCTVFAYGATGAGKTHTMLGSSDNPGVMYLTMKELFGRIDLIKEEKVFDMAFSYLEVYNEQIRDLLANSGPLAVREDGAKGVVVQGLTLHQAKSAEHILEALDFGNRNRTQHPTDVNASSSRSHAVFQIYLRQQDKTASLNPNVRVAKMSLIDLAGSERASATNAKGARLREGANINRSLLALGNVINALADPKSKKPHIPYRDSKLTRLLKDSLGGNCRTVMIANVSPSSLSYEDTHNTLKYANRAKEIKSSLKSNVVSLDSHIGQYVVICEKQKAEIILLKQKLKEYEERKVEPPTLNPISSQRRAEFEKMSDSLRSAFEAQRLVRQEQLDIEKQLKENRMRLSHRQEWHQQSLLFFPDKVEKATCKYERKLASLQSQQQHLQQRLEESKRRFQENKGWLHRIENEMKLLGHNGQTPEELKKDLQCYRLQLQVSDLQRHIEHMSYLINVQDQENKYTHKLVHALLPAYHRQYLALKAAGIATATDESIQEELEHLVLRERGVVWADQETTEVKNEPDGCPEHTCLQPILSFSHLITHQSTPCSSEKHTRRVSQQLKTSQSPKGPSPAEKHAAKAEPPAKKPIRRKLAVSPPKPGSDGTGPALPRCPPALQEVLRQEGMFPLQYTPETHSSKAPNSIDPNCTFEVGQGEQESTPANATVILSPGSPEPAGASGLSQLQVLRANNSNEAPKRLEIPSLLDLRRSKPSYMAMTSAAHGKRKLNCTGKETGLDGLQGAAAPKRIKRDLSDTSKPLRVRRFGASSENDPSRRVVRSISEGNLHLLGPRKPKAALLGPAALFKKVTKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTY4</accession>
    <name>A0A3B4CTY4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Stress-activated protein kinase JNK</fullName>
            <ecNumber evidence="13">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MAPK10</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, and thus regulates transcriptional activity.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. May play a role in the regulation of the circadian clock.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTY4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014206"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022015.2">
        <property type="protein sequence ID" value="ENSPNAP00000014206.2"/>
        <property type="gene ID" value="ENSPNAG00000020204.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153692"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048484">
        <property type="term" value="P:enteric nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07850">
        <property type="entry name" value="STKc_JNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000009">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000210">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008351">
        <property type="entry name" value="MAPK_JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01772">
        <property type="entry name" value="JNKMAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="86"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="428"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014206.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="486" mass="54973" checksum="F6661A2F2ADCDE68" modified="2024-11-27" version="2">IGDAGCHGNVHCKRIACREPVFMNRHFLYNCSAPILDVKIAFCQGFGKQVDVSYIAKHYNMSKSKVDNQFYSVEVGDSTFTVLKRYQNLKPIGSGAQGIVCAGYDAVLDRNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIISLLNVFTPQKSLEEFQDVYLVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPSPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDCLFPADSEHNKLKASQARDLLSKMLIIDPAKRISVDEALQHPYINVWYDPSEVEAPPPQIYDKQLDEREHSIDEWKELIYKEVMNFEERTKNGVVKGQPSPSGAAVNSSESLPPSSSVNDISSMSTDQTLASDTDSSLETSAGPLGCCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CU17</accession>
    <name>A0A3B4CU17_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">FAT atypical cadherin 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000021807.2">
        <property type="protein sequence ID" value="ENSPNAP00000014019.2"/>
        <property type="gene ID" value="ENSPNAG00000023566.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157733"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000010">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000152">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000154">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000015">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000021">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000026">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000037">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000041">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000051">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000052">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000071">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000075">
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000089">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000161">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000058">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000059">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000061">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000084">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000057">
        <property type="entry name" value="protocadherin Fat 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF438">
        <property type="entry name" value="CADHERIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 15" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043489923" description="FAT atypical cadherin 1a" evidence="18">
        <location>
            <begin position="17"/>
            <end position="4571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="4207"/>
            <end position="4230"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="28"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="143"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="361"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="459"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="570"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="713"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="818"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="923"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1037"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1135"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1261"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1349"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1470"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1576"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1681"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1779"/>
            <end position="1892"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1893"/>
            <end position="1997"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="1993"/>
            <end position="2094"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="2095"/>
            <end position="2195"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="2196"/>
            <end position="2296"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="2297"/>
            <end position="2403"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="2404"/>
            <end position="2504"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="2505"/>
            <end position="2608"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="2609"/>
            <end position="2709"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="2712"/>
            <end position="2817"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="2818"/>
            <end position="2927"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="4571" mass="503693" checksum="CF6E6A9FBD796DDE" modified="2024-11-27" version="2">[encoded data, 4571 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZJ7</accession>
    <name>A0A3B4CZJ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">tRNA-dihydrouridine(47) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="19">1.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">tRNA-dihydrouridine synthase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">DUS3L</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the synthesis of dihydrouridine, a modified base, in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Mainly modifies the uridine in position 47 (U47) in the D-loop of most cytoplasmic tRNAs. Also able to mediate the formation of dihydrouridine in some mRNAs, thereby regulating their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(47) in tRNA + NAD(+) = uridine(47) in tRNA + NADH + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:74443"/>
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        </reaction>
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            <dbReference type="Rhea" id="RHEA:53366"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>5,6-dihydrouridine(47) in tRNA + NADP(+) = uridine(47) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53360"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
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        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:53362"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
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            <dbReference type="Rhea" id="RHEA:69853"/>
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        <reaction evidence="16">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
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            <name>FMN</name>
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        <text evidence="19">Belongs to the dus family. Dus3 subfamily.</text>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:mRNA dihydrouridine synthase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:tRNA-dihydrouridine47 synthase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Znf_CCCH"/>
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        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 19" id="KW-0288">FMN</keyword>
    <keyword evidence="6 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="9 18 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="21">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="18">
        <location>
            <begin position="113"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="43"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="232"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045365"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048266"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU291113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="655" mass="73629" checksum="BE95B0373A992992" modified="2018-12-05" version="1">MEAAVDRSGEGADCPAVPQTEKGEAALKAQYITTREKFHEFLAAGLKDQKGDGEESAEASTDGDNGTAEEPTGEPESKKRKADDEAQAEDNKQDKKKMKGQNKSRPHMKPHSYDDRRLCPSLVQERDSKCFYGDKCKFIHDVADYMSTKPADLGDRCYLYDTFGKCVFGITCRFASAHMGPDFKNLVKEEVCKTLGQKEMVRNSLDKELQRRLRKRQVTFSRSETYLKSIGAGKAKGGANGKKSGSENKHATTDAVAEVEKQCVSEQSDTAADLKTENSQSPLVKTIGPLTDADIIKLRPCEKKQVDFRDKLYLAPLTTCGNLPFRRVCKRFGADITCGEMAMCTNLLQGQASEWALLKRHHTEDLFGVQLEGCFPDTMTRCAELLNQNIDVDFVDINSGCPIDLVYKKGGGCGLMTRTNKFEQIVRGMNSVLDVPLTVKIRTGVQQNCNIAHKLIPELKKWGVSLITLHGRSREQRYTKIADWDYIDTCSRLASPVPLFGNGDILSYEDALKARQTGVSGIMVARGALIKPWLFTEIKERRRWDISSGERMDILRDFTNYGLEHWGSDTQGVEKTRTFLLEWLSFTCRYIPVGLLERVPQKINERPPYYLGRDYLETLMASQHVGDWVRISEMLLGPVPKNFSFLPKHKANAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4Q3</accession>
    <name>A0A3B4D4Q3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017566980.1">
        <property type="nucleotide sequence ID" value="XM_017711491.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D4Q3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019332"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029058.2">
        <property type="protein sequence ID" value="ENSPNAP00000019332.2"/>
        <property type="gene ID" value="ENSPNAG00000005949.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435564"/>
    <dbReference type="GeneTree" id="ENSGT01110000267168"/>
    <dbReference type="OMA" id="QLCHKEE"/>
    <dbReference type="OrthoDB" id="10015690at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000130">
        <property type="entry name" value="Chemokine (C-C motif) receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF730">
        <property type="entry name" value="CHEMOKINE XC RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="50"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019332.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="339" mass="38491" checksum="A0727DCD793AD49A" modified="2024-11-27" version="2">MENSSDAEGGPLYEYSEEYDEVCYKETVAKVGSIAVPLFFSIVVLLSLAGNILVLVILGLYENFRSLTNIFIINLAVSDLMFTLGLPFWSSYFIWGWTFGDAVCKGVNFVFSAGYYSSIVFLMLMTIQRYAAVVHPMSKWEGGKQFAAVPILAWVVSISAAVPAVLLSKVMADPEDPNKLYCEYNSMKATVAVTYEQNIFFLSAFLVIGFCYIRILQTVLNSRARKRHRTIRLIFCIVAVFFIGWAPYNIVIFLQTLTYHQIDPFRECYVTDSLEYADYACRLLAFSHCCLNPVFYVFVSVKFQNYLKMIVQKLFHGQSNTDLHQPRNAPVPCSQGSMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D552</accession>
    <name>A0A3B4D552_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cadherin-22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pituitary and brain cadherin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. PB-cadherins may have a role in the morphological organization of pituitary gland and brain tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017574118.1">
        <property type="nucleotide sequence ID" value="XM_017718629.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397430.1">
        <property type="nucleotide sequence ID" value="XM_037541533.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397431.1">
        <property type="nucleotide sequence ID" value="XM_037541534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D552"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028233.2">
        <property type="protein sequence ID" value="ENSPNAP00000018665.2"/>
        <property type="gene ID" value="ENSPNAG00000025182.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439955"/>
    <dbReference type="CTD" id="567936"/>
    <dbReference type="GeneTree" id="ENSGT00940000159567"/>
    <dbReference type="OMA" id="QTIMMGN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000007">
        <property type="entry name" value="Cadherin 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000017">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000200">
        <property type="entry name" value="Cadherin 24, type 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000373">
        <property type="entry name" value="Ventral neural cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF272">
        <property type="entry name" value="CADHERIN-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043310331" description="Cadherin-22" evidence="14">
        <location>
            <begin position="33"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="234"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="315"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="424"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="537"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="642"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="35"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="966"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1234"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="966"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1010"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1062"/>
            <end position="1076"/>
        </location>
    </feature>
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        <location>
            <begin position="1234"/>
            <end position="1246"/>
        </location>
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        <location>
            <begin position="1252"/>
            <end position="1270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057645"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00077373"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018665.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1277" mass="138633" checksum="3DD1E1C15AC9FBB5" modified="2024-11-27" version="2">MPRSSSRRSSLLILRVCVCVYVCSKLVKLVGSADDHTHTAQEGGLDLAPRRAARKDDLSSTSSHTETFQSSKAAWREQDTLKNERITNNEASLAPAGPLWERTAPTDRDASQATEIRASISENAGTAVSSLKGNINPNPSALITTTGSISSATDTLQSTNSRAGLGASLQEASSATQRSGIVFQEFPGDSPERGQGLAGMDPRSRSRRSWIWNQFFVIEEYSGPEPVLIGRLHSSMDRGDGRTKYVLKGEGAGSVFVIDGKTGNIHVTKPLDREEKDQYRLIATATDRQTGRPLEASSQFIIRVQDINDNPPVFPHTHYSATVPEMASIGTSVIQVTATDADDETYGNSAKLVYSVVQGQQYFTVDPQSGIVRTAVSDMDRELQSQYVLVLQARDMGGHQGGLTGTTTITVHLSDVNDNPPRFANSMWSFSVSELALPGVEVGRLSATDADLGENARLDFTITDGEGGGNFNITAVNQEGVILLNKVVDYERRSSYTLTVEVQNPSVDPRFLRRGPFKDRATVRITVLNADEPPRFSRSRYRLDVSENCPPACAVGRVAAVDPDTGLSNNIKYSIDPESDPEALFRIAPDSGLITTVKELDREHEQWHNITVIATQRDSPNQVTRVAVAIETLDLNDNAPELDRQYNTAVCDSSGAGQVVQVIRAIDRDQSSYSSPIHFSLASDRSLALNITIQERGDHTASLVLLSSVKPLPRSASPSFHTVKIPIILRDGASEFRSTGTVTLTMCPCQNGGMWAEERKRQTDRGRSEDAQTLMEWERQTVCVPLPSTSALLGLSSAAMLAVLACSSTLLVVAALALSVRRQKSDSLSPLEDDEIRENIITYDDEGGGEADTAAFDIAALKSAPHSMHSALRLSRSIYSQENLDRMYSWAQAPGVDPQRPGSAPLYGRFCYSIHTLPAVRERIGPFDSRRDLTKLTYTLPSAQTQDCGPLDPGCVFTGLAELAKRTDQSRTGDEHQGTESSAENNLKESETGSEVVSESQTLSQDQSLASDQAPVVQSSSQSDSSSSAGHSAGSLTHHPSTAHTSSTSTIVSTVATDDTPSTHTPTEAPINTSQTSAPFRTMEGTLLRGSGVIPLAGTTCLYPKAGDLVGVYALAGRDFTQRVVMPFPGPGGAGLYMGRWGLGRRDGQGPGPGRGGANPLTHRMGDFLQHRLAQVTFDPMQPPYDSLQTYGLEGAGSQATSLSSLESEGERGMEVEEIKEWGPKFHKLLEIFRERETEEEKKEEKEEKEEKEEKKHTEEETMEEKMQEDTKDEAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6M4</accession>
    <name>A0A3B4D6M4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NIDO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6M4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019155"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028799.2">
        <property type="protein sequence ID" value="ENSPNAP00000019155.2"/>
        <property type="gene ID" value="ENSPNAG00000025683.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052749">
        <property type="entry name" value="Alpha-tectorin"/>
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    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160">
        <property type="entry name" value="ALPHA-TECTORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160:SF9">
        <property type="entry name" value="PROTEIN PRY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIDO" evidence="1">
        <location>
            <begin position="90"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019155.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="297" mass="33222" checksum="12E23039ABA69940" modified="2024-11-27" version="2">VCVLIITVCHNIYIFNHFYPYGVGPGDTVNAQSDDDGSSAISLLRPFVFFGKFYNTTYVNNNGHLTFDQPWSSNSPYRFPAHRGRDLIAPFWTDLDNRISGVISYKQYSSGSVLTQATQNINQYFPQLQFTATSVLVATWDRVPYYGENTFQAVLISDGQLSFILMNYGQIFPIISDVQAGYDTDDSSNYFSIPGSFQNNYSVFTYSSNVNVTGRWAFRVDHAHSPAPSLKHAVLLLSSIAAKIFNAEPAPFLVTPTTTHLITSSFTFKGRAPTSCLRFSFKQSSLFCGFNDCDVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7W4</accession>
    <name>A0A3B4D7W4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RALYL</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM HNRPC family. RALY subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7W4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019518"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029386.2">
        <property type="protein sequence ID" value="ENSPNAP00000019518.1"/>
        <property type="gene ID" value="ENSPNAG00000026098.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437237"/>
    <dbReference type="GeneTree" id="ENSGT00940000164239"/>
    <dbReference type="OMA" id="ECGEMTD"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000019">
        <property type="entry name" value="heterogeneous nuclear ribonucleoproteins C1/C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017347">
        <property type="entry name" value="hnRNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF34">
        <property type="entry name" value="RNA-BINDING RALY-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037992">
        <property type="entry name" value="hnRNP-C_Raly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="241"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="287" mass="32098" checksum="565C9AA8C8E1973F" modified="2018-12-05" version="1">MTGKTQTSNVTNKTDPRSLHSRVFIGNLNTTVVKKSDIELIFAKYGKITGCSVHKGFAFVQYASERNARSAVAGENARVIAGQPLDINMAGEPRLYRPKVGLKRPFSALYSGYGFDYDFYRDDFYGRLLDFHGRVTVPPRAVVPVKRSRLLMPSTRRAKPSQPSRGSSCFSPTRALTSSSSCSGTKVKSDQLLTIKRELSQIKAKIDSLLGWLEKMERQHSAETVPHRKCDDVYPSLHNETAHRSAEEADREHFEGECGEMTDGDEEDCDDEGNDKLMENHLSDVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D811</accession>
    <name>A0A3B4D811_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PLEKHB1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017563192.1">
        <property type="nucleotide sequence ID" value="XM_017707703.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D811"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020487"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031083.2">
        <property type="protein sequence ID" value="ENSPNAP00000020487.1"/>
        <property type="gene ID" value="ENSPNAG00000005163.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433257"/>
    <dbReference type="CTD" id="58473"/>
    <dbReference type="GeneTree" id="ENSGT00390000013989"/>
    <dbReference type="OMA" id="FLWMPFL"/>
    <dbReference type="OrthoDB" id="2157866at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13265">
        <property type="entry name" value="PH_evt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000073">
        <property type="entry name" value="Pleckstrin homology domain-containing family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039680">
        <property type="entry name" value="PLEKHB1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309:SF7">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="187" mass="21196" checksum="4F869C937389B151" modified="2018-12-05" version="1">MALLKSGWLWRQSSVLKRWKLNWCDLWIDGSLVFYKTDSRKDYEMRVSLKSTCVNVKYGLECAGVSPPESHPRENLLVVYLKDGSTVLLCANSEDEALAWKLTIMEARRNPVFIYDPYDDTYQSVPVDGHNAVYITPGGSSGGTHHVWVHRERYDDGIGEQIALGLLAGMAAGAALRSFLWMPFWFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8E9</accession>
    <name>A0A3B4D8E9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MCPH1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017549581.1">
        <property type="nucleotide sequence ID" value="XM_017694092.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8E9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019391"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029188.2">
        <property type="protein sequence ID" value="ENSPNAP00000019391.1"/>
        <property type="gene ID" value="ENSPNAG00000006478.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425442"/>
    <dbReference type="CTD" id="79648"/>
    <dbReference type="GeneTree" id="ENSGT00390000018842"/>
    <dbReference type="OMA" id="AMEPRMT"/>
    <dbReference type="OrthoDB" id="2384350at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17716">
        <property type="entry name" value="BRCT_microcephalin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17736">
        <property type="entry name" value="BRCT_microcephalin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17751">
        <property type="entry name" value="BRCT_microcephalin_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000047">
        <property type="entry name" value="Microcephalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022047">
        <property type="entry name" value="Microcephalin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14625">
        <property type="entry name" value="MICROCEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14625:SF3">
        <property type="entry name" value="MICROCEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="6"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="581"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="693"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="776" mass="86236" checksum="FA75352CFB7708C6" modified="2018-12-05" version="1">MTTKGISAAVLKDVVAYVDVWSASKTENYSDPFIQQLQDMGAQVSKTFNKQVTHVVFKHGHQSTWNKAKRLGVKTVSVCWVARCKENAEHVDEGLYPAQHEESTLSQLKKRTHRCMQPRDAPLSTPKRLKRKLDKMMEGLVRSSPIVSDTSPFIIDEENGIVYSPSSRRADTMAQRLREMRAQRENLSPTASQVQESELEDSPKPSLGKTPTDLSKQLLEEEEAPDLLDTRRRSSSDEVDKEYFDLPLTKTTSKRCVKKASSHSDITFNSSSKEPNKASVPPQSKKTRASCSGLTKQSSLDCFVHKTSKNSPSLPKPADGAIEKPRRSSCNSKKSISSSPLSSMESGIVQVPVCLKETKAHLNVAHSRRPSVGTSLPKRNCSAQPKDLTTPKTAQKVSSKKKPAPVVTSFETNPSGGDDSDDDDDDCVFEDYFSSAKNTPGRRVVLVDSSSKAVNLPTFDLEPLNQNRRKSIPQETCTGNTRQKVFSRKDKSEASSSRRGSVKASDEVITKTSGSARESKISAVPVVSRCEEMKQEPPAKRQKRRNQQNSVTSSESKDSKSTSDGLTDSCSEMCSKQGLMKTDRYMKKNRTLVMTSMPTEKQQTVLQVVNSLGGFTVVDTVCESTTHVVSGSPRRTLNVLLGIARGCWILSFEWILWCLEHRQWIPEEPYELSDHFPAAPICRLQQHLSAGEHQQDLFQDQPLMFVSPLSQPPCRSLVELIQLCGGSVCRSIRQAGLCIGQYKGKKPEGTKCLSEQWILDCVTHLVLLPYDNYILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D918</accession>
    <name>A0A3B4D918_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor HES-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Hairy and enhancer of split 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017560329.1">
        <property type="nucleotide sequence ID" value="XM_017704840.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D918"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020852"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031699.2">
        <property type="protein sequence ID" value="ENSPNAP00000020852.1"/>
        <property type="gene ID" value="ENSPNAG00000027813.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000163190"/>
    <dbReference type="OMA" id="MATTHTR"/>
    <dbReference type="OrthoDB" id="6085656at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097150">
        <property type="term" value="P:neuronal stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11461">
        <property type="entry name" value="bHLH-O_HES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000033">
        <property type="entry name" value="Transcription factor HES-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="14">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="156" mass="18070" checksum="A23E8F6B8CB1221F" modified="2018-12-05" version="1">MAPTYLPASDYSKLSNKEKHKLRKPVVEKMRRDRINNCIEQLKVMLEKQFHQQDPNTKLEKADILEMTVVFLKQQLQPQISAPLKAHHDGYSQCWQETMNFLSANSKLDVALQHLNHGQEAQRAAKDLHVSPVAFQPSQVSVKQETSTNRAVWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D948</accession>
    <name>A0A3B4D948_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574022.1">
        <property type="nucleotide sequence ID" value="XM_017718533.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017574023.1">
        <property type="nucleotide sequence ID" value="XM_017718534.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D948"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020882"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040246.2">
        <property type="protein sequence ID" value="ENSPNAP00000020882.1"/>
        <property type="gene ID" value="ENSPNAG00000027839.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439882"/>
    <dbReference type="CTD" id="550371"/>
    <dbReference type="GeneTree" id="ENSGT00940000156466"/>
    <dbReference type="OMA" id="NSKHHPK"/>
    <dbReference type="OrthoDB" id="444540at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051581">
        <property type="entry name" value="Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524">
        <property type="entry name" value="CALCYPHOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34524:SF6">
        <property type="entry name" value="CALCYPHOSINE LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="18"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="187" mass="21219" checksum="0680DFEB21D5FFDD" modified="2018-12-05" version="1">MANALEELRQQCLSRGAAGIKGLGRTFRIMDDDGSKSLDLQEFQKGLETYGMCVGNEKAKQIFTILDKDGSGTIDFNEFLEKLRPPMSSARMQVIKQAFQKLDRTGDGVVTMEDLQGVYNSKHHPKYKSGQWTEEQVFLSFLESFESPYEKDGKVTLEEFVNYYSGVSASIDSDDYFVTMMQNAWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9U7</accession>
    <name>A0A3B4D9U7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450, family 26, subfamily C, polypeptide 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CYP26C1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9U7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020275"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039924.2">
        <property type="protein sequence ID" value="ENSPNAP00000020275.1"/>
        <property type="gene ID" value="ENSPNAG00000005098.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00800000124060"/>
    <dbReference type="OMA" id="MIIHSTR"/>
    <dbReference type="OrthoDB" id="1372046at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF100">
        <property type="entry name" value="CYTOCHROME P450 26C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="61835" checksum="AA852D8C06764CB6" modified="2018-12-05" version="1">MFVVAALCSLVPALLLLLLSRALWGLRWSSARDRSCALPLPRGSMGWPLVGETLHWLLQGSSFHISRRQKHGNVFKTHLLGKPVIRVTGAEHVRKILLGEHTLVSTQWPQSARIILGPHTLVNSTGELHRKKRKILARVFSREALETYLLRLQDVVKCEVAQWCAEAGSVDAYDATRALTFRVAMRALLGLRLEEQRIIYLSKIFEQLMSNLFSLPVDAPLSGLRKGKRARDILHVAMENIIEKKLKKQEQEDEYCDAFDYMLSGAKDNSYNLTMQELKETAVELIFAAHSTTASAATSLILQLMRHPDVAEHVRAELEAEGLITEARGSCRSRCSEGSGTRSKFEKCVDQEEVRPELKAKSLLDKTTFLEESHHLRTHVPYLSLEKLNQLSYLDCVVKEVLRYLPPVSGGYRTVLQTFELDGYQIPKGWSVMYSIRDTHETSPAFQNPELFDPDRFSPERDESKASRFSYVPFGGGVRRCIGRELAVLVLKTLCVELLATAEWTLATETYPRMQTVPIVHPVNGLHVKFNYRNHARNRRESTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAB1</accession>
    <name>A0A3B4DAB1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ring finger protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RNF17</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="42514.ENSPNAP00000020440"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031008.2">
        <property type="protein sequence ID" value="ENSPNAP00000020440.2"/>
        <property type="gene ID" value="ENSPNAG00000027505.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157559"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16442">
        <property type="entry name" value="RING FINGER PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16442:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="7">
        <location>
            <begin position="713"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="7">
        <location>
            <begin position="949"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="7">
        <location>
            <begin position="1217"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="7">
        <location>
            <begin position="1445"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1127"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1140"/>
            <end position="1153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020440.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1588" mass="176423" checksum="E7C883C047A9A5C4" modified="2024-11-27" version="2">MRAKLSPSCERRRSREKMTDAMVACNNCGLSYTLPEDEVVGNVPHVLHCGHIFCTVCLRTLESPRGFIACPECKMGTNVGEEGVDGLQVDSRIIGLIYTAKMNTKKREREGDKHRARRFRSPSASPPDQEQQSTEVSPDVLEVLDEALCKATENLNTLDSLYQTLVTGIQAQMIKEKTRITKEIEDCVEKAIGILYRRRGVLMSEIGSVEQLFSAGWDECQRVEDRRKELFTAIQKARHVRQVPSLGTYCHLDKILKTLQTPVDAESYDMSCLTVCSELKCILQMDCLTESVKNFLYVMDGNVKLVSKESPGPPPAERLCRRPVRGGGERSGERRGGRSSLRAPGSHRTARASSGLLKLPGAGSAPKQNSAFSPNIIIEEIIEETDSGAERDGMALEPKHIEIQNVQRRNRKTGQDRMPVPQPKALQEWVVLTHIINPTHFYVRHVSERKAGTALARKINKLCSGEESCFTAEDEINTGVLVLVRWKDDVWCRAVVCELFQNGCLESVTQCPAAEVARLRVFFQDYGFSKGFSFSGEGSLSGLNECLRKADVAAKAELHRWAPQAIKCSLKDIIPSDLVNGWSREACDEMRRVIGSSAVEMQVFGEDRDTLLVDLKKPSVDDTLLSLREHLVFMELARFYSPLAAPGGGKPLLFYPPVYPKLNTELPAVVSHVNTPSDFYIQLVDNMEFLLLNSKLQDCYGSPGSDSELQIYCPVQGQACVAIYDEKDWCRAQVTGFPGGRMVEVRYVDFGNRKTLSVNDLRQIKDEFFALPAMAVWCSLADVLSVGDSWSDESIQVFRKLTEQKLVIVVVTKLVPQSAALPVRLFEVSESFTELQASIGETLVRQGLACWSKQTQLKDPPPLEAAVWDPPLDEVTSSPAELTSHTDEPSELLLSLILPTCLKDLRVKVTHVTSPGHIYVQLLQFDAHLKRVHDLLKREFSKSDLQEVDWKVDMPCAANVNGVWERGKVCTVSSSNISEVLRCDFGNTVKLHTSNLRPLLPELVGSLLLECCLSDIRPAGGRSTWTATACDFISYYLTGATAVMTIKDPCARPALVSLFCSNRSGQDVSIADFLISEGLALRERSVRVPPALKPASAECEESNPESGVTLLVQDECVPALEESVCEEGGVTNETPTPSYSCPPRPAPRTAPPPERVKTQAYLPPELPPCGHTLMSISAVSDDGVIYAMTPQSECEFERLMECLQQHIKTLPQQKRYNWKSVLGCAVMGSDMLWYRGQVLEVIRGHVKVRYVDQGIVENIPVCHVYPVVLCEDVPQLCVPCQINGVVPIGGAWQWDAVALMKDLLLGRSVSVQVIELPENLRGMVTVEIMLDGMALSRIMVHHQHATIDPPISSLEDYVVKPPAPDLDNWDLNTEGLEEPQTVLGVYTNPRLQDKGKHFRVTIKHIRTPNEVFLSLLDLTASEQEPLVEALQQVNRDIGSLTQLTDFPIEGPCLAEYSDGKYYRAKLLGFAELNPCVKILVRHVDFGSDDILPPCKLSCLPPALLRFPCEAVCVRLAGFKPPRVCVELERISYRPEWSMKSMLEMIDLLHGDLSAVVTAVEPQLTVVLYNMDGTLVHMPLVEKGLADYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCU9</accession>
    <name>A0A3B4DCU9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037392611.1">
        <property type="nucleotide sequence ID" value="XM_037536714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCU9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021310"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032490.2">
        <property type="protein sequence ID" value="ENSPNAP00000021310.2"/>
        <property type="gene ID" value="ENSPNAG00000005170.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412665"/>
    <dbReference type="GeneTree" id="ENSGT00940000154022"/>
    <dbReference type="OMA" id="WSYTVTV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000022">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF43">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="10 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12 16 17" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="20">
        <location>
            <begin position="577"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="145"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="327"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="660"/>
            <end position="667"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021310.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="919" mass="104055" checksum="5BD98418EC7DCB00" modified="2024-11-27" version="2">MYAFYSLLVYIFYTVFKKEEEAPLEGASGESPEEPGHVSMETGSRRRGGPAPRLGKRGCDRVNDSSSSGSSDSDASCLSDEDDDIDEGHATPAKTPLAAFLSFKQETERRRTSSGPAELGEKASESPLVSVMSHLLSFLEQYSHLQQLQQQAEQYRLQLRRHRIQHRRKMKALRSSYRQRLRDKNSLINSLEEAISQQTSPSPDSDSSPAGLHKLVQSVCGLQGERRQLRVELGLLHSQLEQREQDKHSKVQAFQQQIDELKSCIEEREEELSRLRTSSGATDSEKRVLCLSAENESLKHSLSVTQGLLQQLSIIPSQSSSVLIKENENLRSRVLQLEGALQQRAEQLSRLEQNEWQRGEELRRREERVRELQLELDRERSREPVVKYVTQTLEVESPSTLRQLADERQRNEQLRERLSSQSERCRQLEENIRRSDEVSCNLQHKIAAYEREIGTLQAELLKEIGHLEEKKDEAVKAASSCSQEHLQSLQDQFMTIQRRLNALPPTLRSMKTDYASLRSQVRNFSDFYGAAIKEAKKQISAAITEMSEANKDLLEKYRKEVALRRKYHEQLVELKGNIRVLCRVKPVLKEDEHEEGQAVVVTTDPHNESALTVLSKGKAKTFELDKVFHPQATQEEVFQEIEPLITSCIDGYHVCIFAYGQTGSGKTYTMEGPVENPGINQRALKHLFNEIEERKDMWTYAVTVSSVEIYNEVLRDLLSKDGEKLDIKINPDGTGQLHVPGLRVKEVKSFQHIKKILATARRNRITFGTQMNQHSSRSHALLTVTVQGVDLATGTKSSGKLNLVDLAGSERVWKSGAEGERLKEAQNINRSLLALGDVIQALRGRQTHIPFRNSRLTYLLQDSLGKGNKTAMVVQISSLERNVGETLCSLKFAQRVCKVELGPAARKIESAGHRGENVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCW1</accession>
    <name>A0A3B4DCW1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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            <begin position="1"/>
            <end position="66"/>
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    </feature>
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            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="50"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="2">
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            <dbReference type="Ensembl" id="ENSPNAP00000020774.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="117" mass="13335" checksum="C1575F0E3FDDDC18" modified="2024-11-27" version="2">LIQKDESTCHTETTHQEAPAHKEERAPHETTQKRGRGRPRKQLADPGGKVSKRGRGRPRKAKVSVSLKSSKIFSAQEQLKKAQIKKENANKLQATQLKQIYHVCSLLHNNYRANYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDM9</accession>
    <name>A0A3B4DDM9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tbk1/Ikki binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">AZI2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017559423.1">
        <property type="nucleotide sequence ID" value="XM_017703934.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000021166"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032241.2">
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        <property type="gene ID" value="ENSPNAG00000006491.2"/>
    </dbReference>
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    <dbReference type="CTD" id="64343"/>
    <dbReference type="GeneTree" id="ENSGT00940000153704"/>
    <dbReference type="OMA" id="PWPSQSC"/>
    <dbReference type="OrthoDB" id="8744179at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051891">
        <property type="entry name" value="TBK1-IKBKE_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14432:SF6">
        <property type="entry name" value="5-AZACYTIDINE-INDUCED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14432">
        <property type="entry name" value="PROSAPIP2 PROTEIN/5-AZACYTIDINE INDUCED GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="274"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="375"/>
        </location>
    </feature>
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        <location>
            <begin position="44"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
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        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="365"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="41811" checksum="7764A7BCEE4C0D7E" modified="2018-12-05" version="1">MESPLVDDDICILKHETAYMAESPVAVCSGDESVASHFALVTAYEDIKKRLRDTEKENTLLRKRVKQLEDKLFRPEAPPSEGPQYMNKAFSAYRGIYMEKKDLQAELNKLKKERAESERMLTEQLQAKELEILQLRSEMETSQVMKSLSETQDYWQVDRSSCSETGVQLHTLQEELDQLKLENSRLQKLCSATPDVSDSSVVKNGEGQEVSLDSRAVAVMRSYEDMCSEMSRMRTLVKTQSDLMKKLRDRPLPPVLRRAASALPVQCLDDVETNSSPVRVTVPRPPSAPPLPSSGGCPGASPMAENLQESLWKTQWPLQRPAPLGSPGGPPTLQNHGTPSLDDGSWSIPSHPRPCDALFWEGPKSSGSSSEWAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJW8</accession>
    <name>A0A3B4DJW8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJW8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023229"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013222.2">
        <property type="protein sequence ID" value="ENSPNAP00000023229.2"/>
        <property type="gene ID" value="ENSPNAG00000007748.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167680"/>
    <dbReference type="OMA" id="GMCLPCF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904293">
        <property type="term" value="P:negative regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904153">
        <property type="term" value="P:negative regulation of retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904240">
        <property type="term" value="P:negative regulation of VCP-NPL4-UFD1 AAA ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031632">
        <property type="entry name" value="SVIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055366">
        <property type="entry name" value="SVIP_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35269">
        <property type="entry name" value="SMALL VCP/P97-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35269:SF1">
        <property type="entry name" value="SMALL VCP_P97-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15811">
        <property type="entry name" value="SVIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023229.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="129" mass="14410" checksum="30478923CEC6F735" modified="2024-11-27" version="2">MPGRMQKSMGMCLPCLGGAADDVVVTPDPETRRRQLAEAAEKRQKESTYRGIKNPEAVEKKKKKQEEMEKQAMSTSPSGGGGLKVNPVGKCTLDFNQLYPLCSFESIVFTQYDYEYVICFMYSASSLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLI1</accession>
    <name>A0A3B4DLI1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450, family 2, subfamily AD, polypeptide 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLI1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023971"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011339.2">
        <property type="protein sequence ID" value="ENSPNAP00000023971.2"/>
        <property type="gene ID" value="ENSPNAG00000032704.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182879"/>
    <dbReference type="OMA" id="ITANDFH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000004">
        <property type="entry name" value="cytochrome P450 2D15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008069">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP2D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF309">
        <property type="entry name" value="CYTOCHROME P450-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01686">
        <property type="entry name" value="EP450ICYP2D"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023971.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="56304" checksum="23B79C3056C9E3B9" modified="2024-11-27" version="2">VMILQCLFSLDFMSCLIGIFLFLLLVDIIRNKNPSNFPPGPRPLPFVGNIFTGLDFRTIDKLAEEYGEIFSLRWGSEKMVFISGYKMVKEALVTQLDSFADRPIIPLFHPFFKGLGVVLSNGYLWKMHRKFTNIYLRNFGEAKKNFERSIQQESVFLCEAFKEEKGPFNPHFYVNNAIANIISAAVFGHRFEYHDERFLAVLRLDSEALVLAGSFRAQLYNAFPRLFAYVPGPHKKIFSNYGKITDFLRDEIKKHKEDFDPSNPRDFIDAYLIEMEKVCLKWVILAKSTFFVLATLDMLEAGTETAAATIRWGLLFMMKHPEIQKKVQEEIDRVIGQSRQPSMADKPNMPYTDAVIHEIQRAGNTVPLGFPKRASRDAKLGGYFIPKGTAVTTNLSSVLNDKTEWETPNTFNPGHFLDDQGQFRKRDAFLPFSAGRRVCIGEPLAHIELFLLFTSLLQRFTFAPGPGEKLSLEGLMGFMYVPSPYRMCVIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMJ6</accession>
    <name>A0A3B4DMJ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase M24 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553360.1">
        <property type="nucleotide sequence ID" value="XM_017697871.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMJ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024685"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009511.2">
        <property type="protein sequence ID" value="ENSPNAP00000024685.1"/>
        <property type="gene ID" value="ENSPNAG00000009339.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427609"/>
    <dbReference type="CTD" id="368737"/>
    <dbReference type="GeneTree" id="ENSGT00940000154281"/>
    <dbReference type="OMA" id="SRMFYSE"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="CDD" id="cd01089">
        <property type="entry name" value="PA2G4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000029">
        <property type="entry name" value="Proliferation-associated 2G4, a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000008">
        <property type="entry name" value="Proliferation-associated 2G4, b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004545">
        <property type="entry name" value="PA2G4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00495">
        <property type="entry name" value="crvDNA_42K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF11">
        <property type="entry name" value="PROLIFERATION-ASSOCIATED PROTEIN 2G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="3">
        <location>
            <begin position="21"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="356"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="392" mass="43451" checksum="E7ABCD26F9236B84" modified="2018-12-05" version="1">MSDDEQQEQTIAEDLVVTKYKMGGDIANQALRLVIDAAKPGVSVLSLCEKGDAYIMTETGKVFKKEKDMKKGIAFPTCVSVNNCVCHFSPLKSDPDYTLKDGDLVKIDLGVHVDGFIANVAHSFVIGTSKEAPVTGRKADVIKAAYLCAEAALRLVKPGNQNTQVTEAWNKIAHSFKCNPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLISTGEGKARDGGQRTTIYKRDPSKQYGLKMKTSRIFFSEVERRFDAMPFTLRAFEDEGKARLGVVECAKHELLQPFSVLNEKEGEFVAQFKFTVLLMANGPLRITSGPFEPELYKSEHDVQDPELKSLIQSSASRKAQKKKKKKASKTAENATGQPVENEVAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMM8</accession>
    <name>A0A3B4DMM8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Voltage-gated hydrogen channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Hydrogen voltage-gated channel 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HVCN1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017566737.1">
        <property type="nucleotide sequence ID" value="XM_017711248.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017566738.1">
        <property type="nucleotide sequence ID" value="XM_017711249.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017566739.1">
        <property type="nucleotide sequence ID" value="XM_017711250.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMM8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024344"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010346.2">
        <property type="protein sequence ID" value="ENSPNAP00000024344.1"/>
        <property type="gene ID" value="ENSPNAG00000001223.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435416"/>
    <dbReference type="CTD" id="84329"/>
    <dbReference type="GeneTree" id="ENSGT00940000159403"/>
    <dbReference type="OMA" id="WEDEELH"/>
    <dbReference type="OrthoDB" id="427456at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030171">
        <property type="term" value="F:voltage-gated proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010043">
        <property type="term" value="P:response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000054">
        <property type="entry name" value="voltage-gated hydrogen channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031846">
        <property type="entry name" value="Hvcn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46480">
        <property type="entry name" value="F20B24.22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46480:SF1">
        <property type="entry name" value="VOLTAGE-GATED HYDROGEN CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="229" mass="26890" checksum="976919F2D435066F" modified="2018-12-05" version="1">MSRRFLRFFTAVGDNHHSLFQWDHEELHTNGEELEPSKQTHSAALTFRDSMKRLFSSERFQIGVVCLVVLDAIFVLCELLIDLSIIKADEHRIAPQVFHYLSLALLTFFMVELSLKLFAYRMEFFKHKFEVFDGIVVVVSFVLDIVYISREDAFDGMGLLILLRLWRVARIINGILVTVENRAHHKVQKVQESNDRLVQQVNELQEQKARMEQELSRLRALLKQHNIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMU7</accession>
    <name>A0A3B4DMU7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553348.1">
        <property type="nucleotide sequence ID" value="XM_017697859.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMU7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024793"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009223.2">
        <property type="protein sequence ID" value="ENSPNAP00000024793.1"/>
        <property type="gene ID" value="ENSPNAG00000009535.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427599"/>
    <dbReference type="CTD" id="378854"/>
    <dbReference type="GeneTree" id="ENSGT00940000157504"/>
    <dbReference type="OMA" id="CITAIND"/>
    <dbReference type="OrthoDB" id="5870230at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF155">
        <property type="entry name" value="TETRASPANIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="212" mass="23131" checksum="F2CFD40946CB296E" modified="2018-12-05" version="1">MSKVQGGMKCVKYLLFFFNFIFWLSGSLVLAVGLWLRFDPNTSSLLNEDGAPETFFIAVYILIGAGGIMMLVGFFGCCGAVRESQCLLGSFFACLLVIFGAEVAAGVFGFLKKDKVIEEVTNFYRSSISESGNSTSIVTIYHKILECCGDPNTNAECPNAETKDCITAINDFFNEKLFIIGYVGLGIAGVMIIGMIFSMVLCCAIRNNREVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DN79</accession>
    <name>A0A3B4DN79_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-actin monooxygenase</fullName>
            <ecNumber evidence="5">1.14.13.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Mical family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.225" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DN79"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008944.2">
        <property type="protein sequence ID" value="ENSPNAP00000024920.2"/>
        <property type="gene ID" value="ENSPNAG00000001338.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155580"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120501">
        <property type="term" value="F:F-actin monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22198">
        <property type="entry name" value="CH_MICAL_EHBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF51">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51848">
        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15 21" id="KW-0440">LIM domain</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 21" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="22">
        <location>
            <begin position="508"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="23">
        <location>
            <begin position="716"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="BMERB" evidence="24">
        <location>
            <begin position="877"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024920.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1038" mass="118131" checksum="A0B95B7D6FDBDF6C" modified="2024-11-27" version="2">MGDGQQGEGVGGECRPHALFDSFVQASTCRETLRAFQELCRELKLQPGGKPFFYHTLRNRLHYWRAKALWAKLDKRASQKEYQRGRACTNTTCLIIGAGPCGLRTAIELSFLGARVVLLEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEIHVNVEFRRLREPPENQEKQSVGWRAEVHPTSHPVSELQFDVVIGADGRRNTLPGFHRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRNATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRGNVDQNALLAYARQAADFSTSQQLPTLDFAINHYGQPDVAMFDFTCMYASENAALVRQRRGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTGWMVRSWGLGAAPLEVLAERESVYRLLPQTTPENVSKNYSQYSLDPATRYPNINTQLISPAQVKWSLETRGNQVFLCLCADSTARSNKLLSWCQEQTEGYRSVCVTDLTTSWKNGLALCALIHRYRPDLIDYDCLEESEAEVNWQLGLDIAEREFGVSPIMTIQELSMLEESDSLCMVMYLSQLHDLLKDTAPLSGKEWLINVSFSLFVHFLSPQQDEALKDHHDDHDQAVSPGSESRTVTVVGANQSRVRSMATQLLAKFEENTPSPSVTAMRRQVFVCVEFLASMGGSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTLRLSNYTYDQLQGVFIVLHCLQGPNIPSVKYENTMNSNIRSTYYINYHLFQDKPIKGKRRVKPRQSAKVGTPESSCPTEVVGVWVVLPDPSSISLSSTLFQKAGAEEELDAKLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGIAVEKALRGEAGMGKKDDPSLMQQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELDEEKRILGEMLDVVEQRDALVALLEEQRVREREEDSDLEAVMLSKGFNLHWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTZ2</accession>
    <name>A0A3B4DTZ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc transporter ZIP8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC39A8</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTZ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026933"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003862.2">
        <property type="protein sequence ID" value="ENSPNAP00000026933.2"/>
        <property type="gene ID" value="ENSPNAG00000012306.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158926"/>
    <dbReference type="OMA" id="KFSKIAP"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140410">
        <property type="term" value="F:monoatomic cation:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF2">
        <property type="entry name" value="METAL CATION SYMPORTER ZIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043870598" description="Zinc transporter ZIP8" evidence="7">
        <location>
            <begin position="20"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026933.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="47330" checksum="E9C2773323E1E26B" modified="2024-11-27" version="2">MGHLSRLLPAVLGGLLVSASPEQSAFLGDILAVYGQDGLLDHNGINSLLDTIAQENRGENQPVLAQCLSGEEILTHYGLSNLTHLNQDHLGTICPALLNQGLLPSCTLLAAESHTHIEGHVWGYGFLAVTIINLFSLLGLVLIPATKKPYFPKILTYFIGLAIGTLFSNAALQLIPEAFGFDPKKDDYISKAVGIFGGFYMLFFTEKVLKMLLKTESEHDQSHFSLPESPQQNGGTIIAVTSFSTPSSEKTSIASAASEEQVTAPPQESCLQQLGQRLATVKTVAWMITLSDALHNFIDGLAIGASFTVSVLTGFSTSIAIVCEEFPHELGDFVILLSAGMSIPQAIFFNLLSAMSCYVGLVLGILLGSRFAPNVIFALAGGMFLYIALADMFPEMNAIAKAQQQSRRTELVFFLIQNAGLLTGFLVILLITMFAGNINLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVG0</accession>
    <name>A0A3B4DVG0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565802.2">
        <property type="nucleotide sequence ID" value="XM_017710313.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DVG0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027061"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003544.2">
        <property type="protein sequence ID" value="ENSPNAP00000027061.2"/>
        <property type="gene ID" value="ENSPNAG00000012460.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434880"/>
    <dbReference type="CTD" id="402999"/>
    <dbReference type="GeneTree" id="ENSGT00940000158596"/>
    <dbReference type="OMA" id="AEPPGCE"/>
    <dbReference type="OrthoDB" id="5399138at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042461">
        <property type="term" value="P:photoreceptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048923">
        <property type="term" value="P:posterior lateral line neuromast hair cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048924">
        <property type="term" value="P:posterior lateral line neuromast mantle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003893">
        <property type="entry name" value="Iroquois_homeo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF16">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00548">
        <property type="entry name" value="IRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="132"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="134"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="195"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027061.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="466" mass="51958" checksum="804385EA1FD699DC" modified="2024-11-27" version="2">MSYPQFGYPYSSAPQFLMTTSSLTSCCESSSRSVPDSSMATSAQPPVYCPVYESRLLATARHELVYGSPYTSGQGYGNYVAYGTDASAFYSLGAFDTKDGTASAHAGITQAAAYYPYDPTLGQYQYDRYGSMDGGTRRKNATRETTSTLKAWLQEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWPPRNKCSDEKRYGEDEEGSQEDQIKSETNDDESRSREDKDLQLSDLDDFDAIESESSECELKHQFHMNAHPPSTEHLKDTSLKLSIGPSLEGDRDLAKSCLKTAAAEDLERDPGDMRQTKTCFQQGHQVLDNKPRIWSLAQTATSLNQTEYPSCMLRCQPAMPSSPEASSPTSGLDRQQDSPVTTLRNWVDGVFHDPLFRHSTLNQSHTNTSVSWTMTKGSILEMGAPERTAGSNGIKGHTIALQHQEPNKDNMAFPKAMNKMFCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DY44</accession>
    <name>A0A3B4DY44_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein kinase C delta type</fullName>
            <ecNumber evidence="18">2.7.11.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">nPKC-delta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="18">Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with PDPK1 (via N-terminal region), RAD9A, CDCP1, MUC1 and VASP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Cytoplasm</location>
            <location evidence="18">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DY44"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027981"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001164.2">
        <property type="protein sequence ID" value="ENSPNAP00000027981.2"/>
        <property type="gene ID" value="ENSPNAG00000002824.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155327"/>
    <dbReference type="OMA" id="ETHYLEW"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20834">
        <property type="entry name" value="C1_nPKC_theta-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20837">
        <property type="entry name" value="C1_nPKC_theta-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000360">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000003">
        <property type="entry name" value="Protein kinase C delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000049">
        <property type="entry name" value="Protein kinase C delta type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000008">
        <property type="entry name" value="Protein kinase C theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000150">
        <property type="entry name" value="Protein kinase C, theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027436">
        <property type="entry name" value="PKC_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014376">
        <property type="entry name" value="Prot_kin_PKC_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21494">
        <property type="entry name" value="PKC_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000551">
        <property type="entry name" value="PKC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF501104">
        <property type="entry name" value="Protein_kin_C_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="14 18 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 18 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="23">
        <location>
            <begin position="157"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="23">
        <location>
            <begin position="229"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="356"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="24">
        <location>
            <begin position="618"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="362"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20 21">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000551-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000551-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027981.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="690" mass="78665" checksum="84EA06AD96B7AF18" modified="2024-11-27" version="2">MAPFLRIAFNDFNLGSLPPLAEQPFCAIKMKESLSTERGKTLVQRKPTMYPAWKSSFDAHIYEGRVIQVLLMKTAEETLSEATVGVSVIAERCKKGNGRAEFWVDLQPSGKVMMSVQFFVEDADTESKQSVKEEDGILTLNRRRGAIKQAKVHFIKNHEFTATFFKQPTFCSVCREFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTATNSRDTVFQKERFKIDMPHRFKTHNYMSPTFCDHCGSLLWGMVKQGLKCEDCAMNVHHKCQTKVANLCGINQKLLAEALTQVSLKSSTKRPDPTLSDIGIYQDIAKNTPADPSGNSQYGKLWDGTTPTAPSASITHQTRVTFDSFVFHKVLGKGSFGKVLLAELRGRGEYFAVKALKKDVVLMDDDVECTMVEKRVLALAWDNPFLTHLYCTFQTKEHLFFVMEYLNGGDLMFHIQDKGRFDLYRATFYAAEIICGLQFLHSKGIIYSISLCFYRDLKLDNVMLDKDGHIKIADFGMCKENMVGENRATTFCGTPDYIAPEILLGQRYTFSVDWWSFGVLVYEMLIGQSPFQGDDEDELFESIRMDVPHYPRWITKESKDLLEKMFEREPTRRLGVVGNIRAHPFFKTINWSALEKREVAPPFKPKVKAPNDCSNFDREFLSEKPRLSQADKNLIDSMDQMAFAGFSFINPTMETILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E097</accession>
    <name>A0A3B4E097_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DMRT1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E097"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028731"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035124.2">
        <property type="protein sequence ID" value="ENSPNAP00000028731.2"/>
        <property type="gene ID" value="ENSPNAG00000014524.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156489"/>
    <dbReference type="OMA" id="CLMTECS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022114">
        <property type="entry name" value="DMRT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF70">
        <property type="entry name" value="DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12374">
        <property type="entry name" value="Dmrt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="10">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="8">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028731.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="293" mass="32085" checksum="510D503D53DF1A9E" modified="2024-11-27" version="2">MSDEERSKQQLLDDSASLSPSLGAKKQPRMPKCSRCRNHGFVSPLKGHKRFCNWRDCQCQKCKLIAERQRVMAAQVALRRQQAQEEEMGICTPVTLPGTDIVVKNEPGSEYVFPVGTRSPSSSTPGSATSPTASGDRSTLSPSPTFSSREHSETSTELVVDASYYNFYQPSCYPAYYSNLYNYQQYQQMPSGDSRLSSHNVPQQYRMHYYSAASYLGQGLGTAACVPPIFTLEENNPCPEPKAAAFPADGAHDTSLACMSINPMVSAENKPECEANSDTGVFTVDSIIEGAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8J1</accession>
    <name>A0A3B4E8J1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00006084"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU004301"/>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="16455" checksum="73A69B426B4F5D9B" modified="2018-12-05" version="1">MSKNTVSDRFRKVDVDEYDENKFVDEEEGGENQLGPDEAEVDSLIRQYPSEPAVLKNPPINTKNQNVKDRAEGLVLKVLTAFKSSDIEKAVQSLDKNKVDLLMKYIYKGFERPSENSSAVLLQWHEKALSAGGVGSIVRVLTARKTV</sequence>
</entry>
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    <accession>A0A3B4EEZ7</accession>
    <name>A0A3B4EEZ7_PYGNA</name>
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                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="213" mass="24906" checksum="72115BE4A8186855" modified="2024-11-27" version="2">SPYSPYNQYSPYNPYSPYSLCSPCSPYSPYNRYSPYSPYNPYTPYTPYSQYSPYSQYSPYSPYSPYSPYSPCSPYNHTVHTVYAAHRPIQSIQFIQSIQFIQPIQSIQPIQFIQFIQSIQPIQYIQPIQSIQSIRSMQPMQPIQSIQPIQSILLKWCIQPIQLIQSIQFMQSIQPYIQPIQSIQSIRSIQPMQPIQSIQPIQSILLKWSIQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHW3</accession>
    <name>A0A3B4EHW3_PYGNA</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="INSYN2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28682:SF2">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        </location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJ88</accession>
    <name>A0A3B4EJ88_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine rich adaptor protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LURAP1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_017697935.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJ88"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035286"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028050.2">
        <property type="protein sequence ID" value="ENSPNAP00000035286.2"/>
        <property type="gene ID" value="ENSPNAG00000025167.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427644"/>
    <dbReference type="GeneTree" id="ENSGT00530000063790"/>
    <dbReference type="OMA" id="KQGAPIW"/>
    <dbReference type="OrthoDB" id="8911462at2759"/>
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        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="LURA1/LRA25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037443">
        <property type="entry name" value="LURAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33767:SF2">
        <property type="entry name" value="LEUCINE RICH ADAPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33767">
        <property type="entry name" value="LEUCINE RICH ADAPTOR PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14854">
        <property type="entry name" value="LURAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035286.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="32670" checksum="B53F8F8AFE6CD471" modified="2024-11-27" version="2">MDEVAVNECVPDLRDLEVKVGRKTPEGLLKWMRQEAGLQGHPKPSCPDSGQSEKMGLDEKIRRLKVEMAHLRAADVRILQQLLALHEGIEAVKWLLEERGPLTSHCSSLTSSQYSLGEGPDTSWRGSWSSLQDPHDKLDNISIGSYLDTLADDLDEYCPSSGADSVVCPAQLSTETSQAKNQKVKPDVPKVDPQVVQAKTAPEAGRPSSVINRDSNNPESNKSDTGVLDSALKRAAEMDSPKARLAEKLGKNLSPKSKTYKNGKVELESCKMNGKVHLEYDAHWRWVQSQDDVTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJH6</accession>
    <name>A0A3B4EJH6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hyaluronidase</fullName>
            <ecNumber evidence="10">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SPAM1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 10">Belongs to the glycosyl hydrolase 56 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJH6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029926.2">
        <property type="protein sequence ID" value="ENSPNAP00000036040.2"/>
        <property type="gene ID" value="ENSPNAG00000027273.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230364"/>
    <dbReference type="OMA" id="PFLWVWN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030214">
        <property type="term" value="P:hyaluronan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000065">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018155">
        <property type="entry name" value="Hyaluronidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769">
        <property type="entry name" value="HYALURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769:SF20">
        <property type="entry name" value="HYALURONIDASE PH-20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01630">
        <property type="entry name" value="Glyco_hydro_56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038193">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00846">
        <property type="entry name" value="GLHYDRLASE56"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="8">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="55"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="376"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="432"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU610713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036040.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="497" mass="55905" checksum="40C915704A265063" modified="2024-11-27" version="2">MSKDSVFVLPILSLSLTGVFTLSTILTSQSLPSTASPLFDGQPFMVIWNIPDLVCNRHNISLDTSSFHSVNNPAKVPDQFLILFYADRLGLYPYVDVESRRQFNGGIPQKGNLQASLEKARADIIHYIPSGSSPGLAVIDWEEWRPLWERNWGSKRIYRALSISYARHKFPSLTLRQAVALAKRRFQSAAKSYMLETLTLGTRLRPRYLWGFYLFPNCYNYGWEQPGYTGHCSKEVQKQNEMLLWLWEVSTALFPSIYLQKSLGDHRSAALMVRNRVQEALRVAALPKRTYTAPVYVYARPVFTDQNKRLLSQGDLVSTLGESAAVGASGAVLWGASADYNDKASCEAFSAHLDSTINPYITNITTAARLCSNFLCQGKGRCVRKDYNSDDYLHLSAGSFSIRRGPAGRYEALGKPSLTDLQAFTTAFTCQCYAGLKCEPETLILLSEKKLPRRLRSTNTTRFSVIHTVPKRQPAHCSRSDSAFHTVFKKDRSSRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK96</accession>
    <name>A0A3B4EK96_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted phosphoprotein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SPP1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK96"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036198"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030353.2">
        <property type="protein sequence ID" value="ENSPNAP00000036198.2"/>
        <property type="gene ID" value="ENSPNAG00000004966.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000024390"/>
    <dbReference type="OMA" id="YPGDYKS"/>
    <dbReference type="OrthoDB" id="8958619at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002038">
        <property type="entry name" value="Osteopontin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10607">
        <property type="entry name" value="OSTEOPONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10607:SF1">
        <property type="entry name" value="OSTEOPONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036198.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="28548" checksum="AFE7AAECB8CA37D1" modified="2024-11-27" version="2">IIYLSPLQYKVYMIALNFYNEYNYKTISNVEQSNLTDIQFFLLNDSSESAESGETEATTVPPVTVEPTLDPIIDNGRGDSMGYPSDYKKTIIYVDANDIEKIPFPYKSNGPDKTDDLNSISMKTSHFDGQEANDVEKHLKVYKALQVHNEVLEEDTSTPEEESQGLEVASRTEDEGPSAKQASLGSEESASASDANGESASASASQEETEESDSSKSSEEATATPGAADTENDSSQSSESQEDDSAEQAMETTTDVPDVIIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKL0</accession>
    <name>A0A3B4EKL0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EKL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032388.2">
        <property type="protein sequence ID" value="ENSPNAP00000036997.2"/>
        <property type="gene ID" value="ENSPNAG00000006870.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272407"/>
    <dbReference type="OMA" id="DRRNSKW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027814">
        <property type="entry name" value="DUF4562"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34833">
        <property type="entry name" value="GENE, 17359-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34833:SF1">
        <property type="entry name" value="GENE, 17359-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15123">
        <property type="entry name" value="DUF4562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036997.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="125" mass="14057" checksum="37DCDF29DA2DF428" modified="2024-11-27" version="2">RSIGVGPLSPEATGDLTYLYRAAPHMPAPLPKQCYAGGVGWAVHYGSALNSTTLLSNKQIKLAEIRSALEHRVTHRYQNPWQAPPCFLDRQQAGTRGRLAWNQNIYDNYCQDNNKTFLLSKQDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKT2</accession>
    <name>A0A3B4EKT2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sorting nexin-27</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017557512.1">
        <property type="nucleotide sequence ID" value="XM_017702023.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EKT2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036505"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031166.2">
        <property type="protein sequence ID" value="ENSPNAP00000036505.1"/>
        <property type="gene ID" value="ENSPNAG00000027432.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429939"/>
    <dbReference type="CTD" id="393512"/>
    <dbReference type="GeneTree" id="ENSGT00950000183212"/>
    <dbReference type="OrthoDB" id="10036828at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13338">
        <property type="entry name" value="FERM-like_C_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01777">
        <property type="entry name" value="FERM_F1_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23070">
        <property type="entry name" value="PDZ_SNX27-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06886">
        <property type="entry name" value="PX_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.60:FF:000002">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000061">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000075">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000003">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037827">
        <property type="entry name" value="SNX27_FERM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037833">
        <property type="entry name" value="SNX27_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037835">
        <property type="entry name" value="SNX27_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF17">
        <property type="entry name" value="SORTING NEXIN 27B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="57"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="175"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="15">
        <location>
            <begin position="287"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="575" mass="64693" checksum="3AB137FD870EB57C" modified="2018-12-05" version="1">MADVVDGDDIRSAAPSVIHSSSRGGCGGSGSSCNDAPVTGGHAPPLPVATVASGPRLVRIVKSDSGYGFNVRGQVSEGGQLRSINGELYAPLQHVSAVLPGGAADRAGISKGDRILEVNGVNVEGATHKQVVDLIRAGERELVLAVLSVPQPETDSLDPGDDGSAQSCYDYSDKQAVPISVPTYKHVEQNGEKFVVYNVYMAGRQLCSKRYREFAILHQNLKREFATFTFPKLPGKWPFSLSEQQLDARRRGLEEYLERVCSVRVIGESDIMQEFLSESDENYNGVSDVELRIAMPDKTTVTVRVRKNCTTDQVYQAVVMKVGMDSITASYFALFEVINHSFVRKLAPNEFPHKLYVQNYTSAIPGTCLTLRKWLFTTEEEILLNDNELAINYCFHQALDDVKKGFIKAEEKSYQLQKLAEQRKISMYLSMVRSCEGYNEIIFPHCACDSRRKGHVVTAISIHHFKLHACTEEGTLENQVIAFEWGEMQRWDTDEEGMAFCFEYARGEKKPRWVKIFTPYFNYMHECFERVFCELKWRKEVEEEATDKDNKNCSKDGMCSKNIFQLLRHRRDGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EMQ2</accession>
    <name>A0A3B4EMQ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Progestin and adipoQ receptor family member VI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PAQR6</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564233.1">
        <property type="nucleotide sequence ID" value="XM_017708744.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EMQ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037053"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032540.2">
        <property type="protein sequence ID" value="ENSPNAP00000037053.1"/>
        <property type="gene ID" value="ENSPNAG00000004347.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433890"/>
    <dbReference type="CTD" id="79957"/>
    <dbReference type="GeneTree" id="ENSGT00940000160567"/>
    <dbReference type="OMA" id="TSCCAHT"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF39">
        <property type="entry name" value="MEMBRANE PROGESTIN RECEPTOR DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="47064" checksum="1D66AB02662F616A" modified="2018-12-05" version="1">MKKSPQHLFPVWRGSGGGPGWGWPRYIGWEAGEGEAEPWGFDCPVWCVLCSGGRERLKQACQRGRGALRLPANMLRIKLPRLFDNHQVPKVFREDSIISGYRHPQSSALDCILSSFQMTNETVNIWTHFLPTWYFLWCFCVLCSTENFLTESYTWPLLVYMLLICLYPFTSSCAHTFSTMSSEARHICYFFDYGALSLYSLGCAITYGSYVMPECWVNSWLHQHFVPIAIANTIFCTSMSCYSRFLELQFPHRSKIVRTSAFVVPFIFDNIPLFYRLLLCFSGSCNHTEAVSSHCYHLLFAFLTGFLFASHLPERLAPGRFDYIGHSHQLFHICAVVGTHFQMEAVLADMSSRRSWLMAHSAGPSFLGTLGALALAFLLNLGVIGLFSACLLRTPNLSTSGFTSTPTLQAPATAKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EPL1</accession>
    <name>A0A3B4EPL1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EPL1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037121"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032709.2">
        <property type="protein sequence ID" value="ENSPNAP00000037121.1"/>
        <property type="gene ID" value="ENSPNAG00000028557.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161602"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000009">
        <property type="entry name" value="Transcription factor HES-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="177" mass="19738" checksum="122862E49B78716F" modified="2018-12-05" version="1">NTEEASCLRTLKPLMEKRRRARINDSLNKLKALIVPLVGKDTSRYSKLEKADVLEMTVRFLRELPSSPAKDQPDSYREGYTACLQRVSALLPHTGLDREAGLRVNEFMQRAMLSASPACQHCCGRSASVAPQTQQRLRNLQAANAPRVQSTSSLATPAPSRPQPAAQALINNMWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EPT0</accession>
    <name>A0A3B4EPT0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Solute carrier family 25 member 53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLC25A53</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EPT0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037191"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040619.2">
        <property type="protein sequence ID" value="ENSPNAP00000037191.2"/>
        <property type="gene ID" value="ENSPNAG00000028612.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161713"/>
    <dbReference type="OMA" id="LGYYRAF"/>
    <dbReference type="OrthoDB" id="2139348at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052465">
        <property type="entry name" value="Mito_NAD+_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46131">
        <property type="entry name" value="SD08549P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46131:SF5">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="20"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="109"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="202"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037191.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="31757" checksum="DA55F4F411C9FAF4" modified="2024-11-27" version="2">TGRCPQHSDGNQDPAVTGSSAWFRSYMHGGTSTVISTLVSFPVYKTVFRQQVHNITVLEALRQLCGEGPLKLYRGIAPPLLMKTLNGTLLFGLQDTILQQLLWTGSVLPRNALPALAGLGVGVVEALIFTPFERVQNILQNSSNDRSLPTLRRILVRLSSETLASGFYRAFLPILARNALGNTIYFGLKDPVSSAFKERGLSPIASSFLSGVVNSMLISLSVYPLSVLVANMQAQVGGESLGLRVCWQQLWKARQFRLAQLYRGGSLVILRSCITWGITTAIYDQLKSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQY3</accession>
    <name>A0A3B4EQY3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ankyrin repeat domain-containing protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ANKRD16</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required to prevent the misactivation of serine (Ser) with tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala), thereby playing a role in translational fidelity. Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and precluding Ser misincorporation in nascent peptides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with AARS; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017567687.1">
        <property type="nucleotide sequence ID" value="XM_017712198.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EQY3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037704"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041166.2">
        <property type="protein sequence ID" value="ENSPNAP00000037704.1"/>
        <property type="gene ID" value="ENSPNAG00000029740.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436006"/>
    <dbReference type="CTD" id="54522"/>
    <dbReference type="GeneTree" id="ENSGT00940000153969"/>
    <dbReference type="OrthoDB" id="4772757at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000336">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF185">
        <property type="entry name" value="ANKYRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="282"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="372" mass="41598" checksum="F284CFB3141DCBE7" modified="2018-12-05" version="1">MDEEKTFKPLIKLTQDGHLSVLKDQIEGESGIYFKQHINAHFGKSGDTLLHYAARHGHIDILRYFIEELGMDIELYNNDYKRALHEAASMSHEQCVQYLIDRGAQIDCLKKADWTPFMMACTRRDLRVIKVLLDHSADPTLHNKDGWNSFHIACREGDPAVIEHLLLVRPEVWRTESKTRRTPLHTAAMHGCLEVVKILLEKCAYKPDKKDSCGVTPFMDAVRNGHLAVAKLLLEKHQASPTAVDILGVQPLHQASVTAQEEALCFLVQDLSVDVNAKATKLELTALHYAAKEGHTSTIKTLLQLGADLHARDKKGRSALHMACIGQHAATAQTLLQLGLTDTEDSTGTMARQLAKKPDITRVFESPEEIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IRS5</accession>
    <name>A0AAR2IRS5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Accepts the ubiquitin-like proteins sumo1, sumo2 and sumo3 from the uble1a-uble1b E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as ranbp2 or cbx4. Essential for nuclear architecture and chromosome segregation. Mediates nuclear localization of vsx1. Required for progression through mitosis during organogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a tight complex with rangap1 and ranbp2. Interacts with vsx1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IRS5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064996.1">
        <property type="protein sequence ID" value="ENSPNAP00000040734.1"/>
        <property type="gene ID" value="ENSPNAG00000018749.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075088"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036306">
        <property type="term" value="P:embryonic heart tube elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000013">
        <property type="entry name" value="SUMO-conjugating enzyme UBC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="158" mass="18129" checksum="93B6492B45B50212" modified="2024-11-27" version="1">MSGIALSRLSQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTLWEGGLYKLRMLFKDDYPSSPPKCKFEPPIFHPNVYPSGTVCLSILEEEKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRMDYEKRVRAQTKRFAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IT49</accession>
    <name>A0AAR2IT49_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Regulator of calcineurin 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RCAN3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572235.1">
        <property type="nucleotide sequence ID" value="XM_017716746.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IT49"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081182.1">
        <property type="protein sequence ID" value="ENSPNAP00000040796.1"/>
        <property type="gene ID" value="ENSPNAG00000034164.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108438738"/>
    <dbReference type="CTD" id="11123"/>
    <dbReference type="GeneTree" id="ENSGT00940000159501"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000092">
        <property type="entry name" value="Calcipressin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300">
        <property type="entry name" value="CALCIPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300:SF6">
        <property type="entry name" value="CALCIPRESSIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="206"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26394" checksum="D3E7614BB1857672" modified="2024-11-27" version="1">MMMRAGGKWLSNGSEALSSDPEEEEDGEEMVFGENDEESEEMMDLSDLPTALFACSVHEAVFEEDRQRERFESLFRIYDDQTSFQMFKSFRRVRISFSTPEAAARARIELHESEFNGSKLKLYFAQVQNSGEDVDKAYLAPPQPMKQFLISPPASPPVGWSQGEDATPVINYDLLCAVAKLGPGEKYELHAGTESTPSVVVHVCESETEEEEDARPKMRIVQTRRPEGPPKVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IT85</accession>
    <name>A0AAR2IT85_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EMC1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IT85"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054998.1">
        <property type="protein sequence ID" value="ENSPNAP00000042928.1"/>
        <property type="gene ID" value="ENSPNAG00000022746.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002461"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000856">
        <property type="entry name" value="ER membrane protein complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058545">
        <property type="entry name" value="Beta-prop_EMC1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25293">
        <property type="entry name" value="Beta-prop_EMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043747863" description="ER membrane protein complex subunit 1" evidence="12">
        <location>
            <begin position="21"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="EMC1 first beta-propeller" evidence="14">
        <location>
            <begin position="20"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="13">
        <location>
            <begin position="765"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="971" mass="109090" checksum="63367A689FB49EDD" modified="2024-11-27" version="1">MAGQLAGFTVSLCLLGFTVAVFEDQVGKFDWRQQFVGEVQFALFDTHSQASKKLLVATEKNIFASLNSRTGELFWRHVDKMGPEGHIDMLLMHGQDAVVVVGNGRLLRSWETTVGGLNWETVLDTGSFQSAAFIGLQDLVKYVAVLKKSAISLHYLSNGHQKWVENLPESDTVQYQTVYSGGNGQVFVLGVVPNSHIVIVEYNIEDGEIMKQNSVAAPWMSSLKSSCVVVGIGVLLCVDPTTESIYTLPLQSEEQTKMTPILLQTLGLEMASGFQPVLMSTQPHPARPPLSEFFLQLGPDHHILLQLKDGHISALRDFNPSYLVSFATTGEKTVAAVMSPKNETVSIHPRNACSINLFSADTGRRLLDTTIIYYMDPNGGKPTKLYVHAFLKKDDSVGYRVLVQTEDLVLNFLQQPGRVVWTREEALADVVTMEMVDLPLTGTQAELEGEFGKKADGLLPMVLKRLSSQLILLQAWLSHLWKLFYDARKPRSHVKNEVTIETLSRDEFNLQKMMVMVTASGKLFGIDSKSGAVLWKKYLENVQPNSVFKLMVQRTTAHFPHPPQCTLLIKDKDTGLGSLYVFNPIFGKKSHISVPALPRPVLQSLLLPLMDQDYAKVLLLIDDQYKVTAFPSTKNVLQQLQEMASSIFFYLVDASQGRLSGFRLRKDLSTELIWEVVIPTEVQKIVGVKGKRANEHVHSQGRVMGDRSVLYKYLNPNLLAVVTESTDSHQERSFVGIFLIDGVTGRIVHEAVQRKARGPVHFVHSENWVVYVYWNTKSRRNEFSVLELFEGMELYNSTVFSSLDRPHPPQVLQQSYIFPSHISTLEATLTEKGITSRHLLVGLPSGAIMSLPKMFLDPRRPEVGSDQSREENLIPYSPEMMIRTEWFINYNQTVSRVRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLLGLFFATMISKRLAEVKLLNRAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITB3</accession>
    <name>A0AAR2ITB3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LCCL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017567207.2">
        <property type="nucleotide sequence ID" value="XM_017711718.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ITB3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085993.1">
        <property type="protein sequence ID" value="ENSPNAP00000042955.1"/>
        <property type="gene ID" value="ENSPNAG00000018672.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435711"/>
    <dbReference type="CTD" id="393442"/>
    <dbReference type="GeneTree" id="ENSGT00940000156473"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000001">
        <property type="entry name" value="Cysteine-rich secretory protein LCCL domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.130.20:FF:000001">
        <property type="entry name" value="Cysteine-rich secretory protein LCCL domain-containing 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051957">
        <property type="entry name" value="CRISP-LCCL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331:SF9">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN LCCL DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331">
        <property type="entry name" value="LCCL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G08630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043826408" description="LCCL domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="8">
        <location>
            <begin position="298"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="8">
        <location>
            <begin position="399"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="251"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="508" mass="57152" checksum="DB673708305FA353" modified="2024-11-27" version="1">MKRPPLPWLCRALLLLSARAAVAIVIPNGTQVEAMLQRYLEKEESWWEARPRGRRAISASDMQLILDLHNKLRGQVYPPASNMEYMVWDTELERSAEDWAHTCRWEHGPPHLLTQIGQNLGAHWGRDRPPTFHVQAWYDEVRYFSYPYPQECNPYCPYRCSGPVCTHYTQLVWATSSKIGCAINVCYNMNVWGMIWAKAVYLVCNYSPPGNWWGHAPYKHGHPCSACPASYGGGCRDNLCYKDDGSQRYYDPEPEENNYIEPEREPARSQVSHPVEPSPTPPPSHSLDRNDVSSTEQMSQQVSCDTKLRDQCKGTTCNRYECPASCLYSYGKVIGSGHYDMQSSICGAGLHAGIIDADGGWLDITRVGRRQRFAKSYKNGVQTLAKNHSANAFTVSKVPVKAIACDVTVAQYCPFKKPVRHCPRLFCPRNCLYDSHASVIGTRYYADRSSICRAAIHAGVIQSESGGYLDVMPVDRRRQYTGSNQNGIASESLQNPTGGKAFRVFAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IW50</accession>
    <name>A0AAR2IW50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017575956.1">
        <property type="nucleotide sequence ID" value="XM_017720467.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IW50"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047392.1">
        <property type="protein sequence ID" value="ENSPNAP00000042274.1"/>
        <property type="gene ID" value="ENSPNAG00000013138.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441122"/>
    <dbReference type="CTD" id="327387"/>
    <dbReference type="GeneTree" id="ENSGT00950000182882"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001891">
        <property type="term" value="C:phagocytic cup"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006911">
        <property type="term" value="P:phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071800">
        <property type="term" value="P:podosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07612">
        <property type="entry name" value="BAR_Bin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000013">
        <property type="entry name" value="Amphiphysin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003005">
        <property type="entry name" value="Amphiphysin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046984">
        <property type="entry name" value="BAR_Bin2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46514">
        <property type="entry name" value="AMPHIPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46514:SF1">
        <property type="entry name" value="BRIDGING INTEGRATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01251">
        <property type="entry name" value="AMPHIPHYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="9">
        <location>
            <begin position="36"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="302"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="157"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="439"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="553" mass="61637" checksum="50BAD962303029C9" modified="2024-11-27" version="1">MAESKHSGNIGGNIGAGAGILAKRFQKSMNRAQEKVLQKLGKTMETRDEQFEQCSANLNKQQMDGTRLFKDVKSYYNAVKVMHETSKRLSQTLKEIYESDWQGLEDLSVIMESEDLLWNDYEEKLSDQVIRTMENYTSQFPDVKERVSKRGRKLVDYDSARHHLEALQNAKKKDEAKIAKAEEEFNKAQSVFEDINKELREELPVLYQSRIGCYVTMFQNISNLRDVFYKEMSVLNHDIYNVMKKLETQHSTKTFIIKGLNSTKSKKRKSLTISAPIPCNTAFPADHPGLSKLSVPDELVAKDTDSNSTPVKAATTTAETSDSDDFSSVDSVPNTPNRQSVCSEGEGVKSGTANESTTEIAADQLVQSFDKEQPGSSAASEQDEEEQSEDALSSAPTSEDRNAPEATLSEGAGEEPEPSQYENEKAAPVPAARSLAPVKVEEAADGEKAESGKEEDQTKELKREKEAVTSQKANSSNPPGFLYKAIALETQESDEGVLLLFTKGDILLIYVEEEEKPEGSVWGVREQDWIQYKDLTLLSGIVMETMIQRINSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWI8</accession>
    <name>A0AAR2IWI8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphorylase kinase</fullName>
            <ecNumber evidence="3">2.7.11.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PHKG2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + phosphorylase b = 2 ADP + phosphorylase a.</text>
            <dbReference type="EC" id="2.7.11.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.19" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWI8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049186.1">
        <property type="protein sequence ID" value="ENSPNAP00000041961.1"/>
        <property type="gene ID" value="ENSPNAG00000008236.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160435"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004689">
        <property type="term" value="F:phosphorylase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000138">
        <property type="entry name" value="Phosphorylase b kinase gamma catalytic chain, liver/testis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000149">
        <property type="entry name" value="phosphorylase b kinase gamma catalytic chain, liver/testis isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002291">
        <property type="entry name" value="Phosph_kin_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01049">
        <property type="entry name" value="PHOSPHBKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="393" mass="45217" checksum="6CF18639678CA324" modified="2024-11-27" version="1">MTRDVLGDELPDWVGAKEFHQKYEPKEVIGRGVSSVVRRCVNKHTGQELAVKIIEITAEKMTAQQLVEVKSSTLKEIHVLNVVQGHPSIITLVDSYESATFIFLVFDLMRRGELFDYLTEKVTLSEKETRSMMRALLEAVQYLHSLNIVHRDLKPENILLDDQGHIKLSDFGFSVQLRPGEKLRELCGTPGYLAPEILKCSMDETHEGYGKEVDLWACGVILFTLLAGSPPFWHRKQMLMLRMIMEGRYQFSSPEWDDRSDTVKDLISRLLVVDPTLRLTAEQALAHPFFRQYQKDDVRLFSPRKTFRVLILTVLGCIRMHCRYNRARPLTRELLARDPYSLRAVRKLIDGCAFRIYGHWVKKGEQQNRAALFQNTTKVYVMDGTIASVSTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J201</accession>
    <name>A0AAR2J201_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J201"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065668.1">
        <property type="protein sequence ID" value="ENSPNAP00000043856.1"/>
        <property type="gene ID" value="ENSPNAG00000032580.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157454"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF9">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="186" mass="20266" checksum="3911EE411A9C3237" modified="2024-11-27" version="1">PTTSPTDVSSLEEILKSVQFNLINLQGAGLEENGASSLLDMILYYESATHLDISSNASLGVSGWQALARLLRQSGCLWRLDACNIPMLEYPAQALSKALLSSRLTVLHLENTCLSGKPLFTLVGALKKNSALQELYLCDNELNSYQDAMQLGELLKYNHTLKALNLSNNTVSDTGMDLYSGPKVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J8H0</accession>
    <name>A0AAR2J8H0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">WNT9A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J8H0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055937.1">
        <property type="protein sequence ID" value="ENSPNAP00000048235.1"/>
        <property type="gene ID" value="ENSPNAG00000011124.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159618"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000002">
        <property type="entry name" value="Protein Wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013303">
        <property type="entry name" value="Wnt9a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF75">
        <property type="entry name" value="PROTEIN WNT-9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01894">
        <property type="entry name" value="WNT14PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="369" mass="41524" checksum="6A8331CDE47EF476" modified="2024-11-27" version="1">MLDGQFWTTPAHPKGTGWSSITPEHLILSVSAMILLTGDKPLSILPLSAQPEDRSVKLCDRLKLEKKQHRMCRRDPGVAETLMEAISMSALECQYQFRFERWNCTLERRYRANILKRGFKETAFLYAISSAGLTHAMAKACSAGRMERCTCDEAPDLENRKAWQWGGCGDNLKYSNKFVKDFLGKRSNKDLRARVDMHNSNVGMKVVKAGVETTCKCHGVSGSCTVQTCWRQLAPFHEIGKLLKQRYETSLKVGSSSNEATGEGEIPQPRAPGHHQLTPEPRPTDPIPRTTDLLHIEDSPNFCRASKYSAGTAARKCYKDKNCEAICCGRGHNTQSRTVTRPCQCQVRWCCYVECKQCTQKEEVYTCKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JSL0</accession>
    <name>A0AAR2JSL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JSL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069004.1">
        <property type="protein sequence ID" value="ENSPNAP00000055013.1"/>
        <property type="gene ID" value="ENSPNAG00000030367.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164914"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF7">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="61566" checksum="7E0DBD2168794445" modified="2024-11-27" version="1">MTFNTEEKHPTRLKRYTQLTSASLKRKSSTEGSLAPPSKQTKLWETQRVSQASVDKVVLKFVGFIDLVQHLRPNTNVMTRNTVVNKVTKASIEMRRKLKAALSEIEFIATTTDCWTAHRRSFIGVTAHWFNPQTLQRSCVALAWKQLKGSHTFSALAGALNDIHTEFNIREIVRTTTDNGSNFLKAFRIYGQTDENNNPEPVGESDGEEDDGGQNDDDDEEESVEGVEFVDAGALLDEDDYLEYQLPKHHRCACHLLNLVSTVDALKAEVNPLYKRVSRTTFAKCSSLWNKSSRSTTASEVIKDHCKLQFLRPVATRWNSLFSAVERIVRITREQGEGALAAVCSELDTPKFTPVELAFLAEYAKTMSPVAKALDVLQGETSVQMGWLVPTITLLRTKLQQLNIMLTDPELIAAAILVPKFKTCWTSDENILKLGLDYIRSHLDCQAENHISEGSQSCEEEDFFSSLKKTSPLETTQQLDAYLGCPIDRAEVLKSFPAVCQLSLKLNTALPASAACERLFTVAGLIFRPKRAHTGSKNLEPAAFVYNFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JSL3</accession>
    <name>A0AAR2JSL3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">1-phosphatidylinositol 3-phosphate 5-kinase</fullName>
            <ecNumber evidence="3">2.7.1.150</ecNumber>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">FYVE finger-containing phosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">PIKfyve</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Phosphatidylinositol 3-phosphate 5-kinase type III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Serine-protein kinase PIKFYVE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">PIKFYVE</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:44681"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57923"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.150"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:13610"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14. VAC14 nucleates the assembly of the complex and serves as a scaffold by pentamerizing into a star-shaped structure, which can bind a single copy each of PIKFYVE and FIG4 and coordinates their activities. Interacts (via chaperonin-like domain) with RABEPK; the interaction recruits RABEPK to the endosomal membrane. Interacts with SPAG9. Interacts with EGFR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Cytoplasmic vesicle</location>
            <location evidence="18">Phagosome membrane</location>
            <topology evidence="18">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="3"/>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069581.1">
        <property type="protein sequence ID" value="ENSPNAP00000054995.1"/>
        <property type="gene ID" value="ENSPNAG00000008358.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156307"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052810">
        <property type="term" value="F:1-phosphatidylinositol-5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032438">
        <property type="term" value="P:melanosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903426">
        <property type="term" value="P:regulation of reactive oxygen species biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04448">
        <property type="entry name" value="DEP_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15725">
        <property type="entry name" value="FYVE_PIKfyve_Fab1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000206">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000057">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000004">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043548">
        <property type="entry name" value="PIKfyve"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037378">
        <property type="entry name" value="PIKfyve_DEP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46715">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46715:SF1">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11 29" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 29" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 28" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="31">
        <location>
            <begin position="177"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="32">
        <location>
            <begin position="392"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="33">
        <location>
            <begin position="1751"/>
            <end position="2084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="60"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="307"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="469"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="924"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1222"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1572"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1752"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="30">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="514"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="30">
        <location>
            <begin position="944"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="990"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="1239"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="1593"/>
            <end position="1607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="1621"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="1754"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="1789"/>
            <end position="1801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00065580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00071350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00076918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00077223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00077675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00081348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2098" mass="236122" checksum="A60D2B56286D0CBC" modified="2024-11-27" version="1">MAAEDKSTSSSSTLDWNVEPPLSPASPSHLTHFKPLTPDQDEPPLRSAYSSLVSLFRFNKEKSGANIAPAKKLGKSDEGRPTSTAEKTESAEASPQGERRNWSSPTLSALGSATHRKHSDLIRRTSTASEGRRKSETPLSSHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSADSGCIGEDLTALSDSPCSVCILEPTEPRTPVSGRKASRNIFLEEDLAWQRKTPIGMRKNLIHQESQNSGLGSRLSAVQEDLGKSPARKRSASVTNLSLDRSGSMVPSYESSVSPQTSRVVAKADHSEEERKILLDSSQLKDLWKKICHSNTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISTRAQAIAIGQALVDGRWLDCVTHHDQIFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQLADESDYITPNSASPSKRTSVSSFQSAVDSDSAASINLNVEQDNVNFHIKKQAKYPHVPPHPAEQKTEFLVTEDGGQNISISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRSCDDDMDIRQLVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMNSYIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIADVRPNLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSRMTQGDLVMSMDQLLTKPRLGTCHKFYLHSFQLPHSECIKTLMFFEGCPPQLGCTIKLRGASEYELARVKEIITMMVCVAYHSQLEISFLMDEFAMPPSLSKSTSFPCLLESTVREEEQEAERESQGNGASVEEDNLELPSESDFEPGFQDVIKGHSRQPSVSESSVKDGESPKVSRNNSSSSLLGGEAEEVKTSTPVSCPVSQRPSVSPPYLIADLEEIAEEEVKEPLDAEGNGQEEGTMVQGVRADSETSLPPAQLFRDPLQDDTGLFVAEQVTSADDRLKSISACFKQELKDIILCISPFITFREPFLLTPAGLRCPSRDYFPEQVYLSPLLNKDFRELDGRRKKLLLKDLAPPACSLSNGTGPPQRPAQVLPSHKLTTTRIVEQLSSSQELARMLADYRAQGGRIRQRDVDPFASPPPTKASVKADSEEEKGSGQNEMAWATKLDCLTPKNHQRLCVLFSSSSTQSHNAPNHCVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMYCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRNCKQATPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYIPVRLFEICLPPPKIFIRNQGPCKTGLQQDLKDFSQKVAQVYAAVDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKLQARIQATGVDTPQQLQAVLESVVIKKQSLCETLQSWNSKLQDLFQLEKGRKRLSVPPSPGRHRQTTNDDSKTSVLESSPRNPSPVVQNGEKEDRHLTTMPSGSGSSSLLQLPSPAEQGSELLTSGPTFPDQDSVSIPEDVFDGHLLGSNDSQVKEKTTMKTILANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEREPSSIIAFALSCKEYKNALEELAKTMLKASGEDASQSVSSGESRVRNSPAKASEGSTSQLGRSSVDLDPLKEPESGDKQKKQTGNPHIELQFSDASAKFYCRIYYAEEFHKMREEVMESTEEEFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPNYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDQGKESCEVVLLDENLLKLVHDNPLYIRSHCKAILRAAIHSDAYFLSSHLIIDYSLLVGRDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGVNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JUN8</accession>
    <name>A0AAR2JUN8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JUN8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077892.1">
        <property type="protein sequence ID" value="ENSPNAP00000053561.1"/>
        <property type="gene ID" value="ENSPNAG00000026470.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156639"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000001">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 5-kinase type-1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF105">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE TYPE-1 BETA ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="5">
        <location>
            <begin position="31"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="485"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="531" mass="60055" checksum="78203B654A9B4A59" modified="2024-11-27" version="1">MRRAVYKMSAVNESEGGGPQKSASKDHKKPTAEAVKGAIQLGIGYTVGNLTSKPDRDVLMQDFYMVESVFLPSEGSNLTPAHHYPDFRFKTYAPVAFRYFRELFGIKPDDYLYSICNEPLIELSNPGASSSWFYLTSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQCGGINIRLVVMNNVLPRSLTMHYKYDLKGSTYKRRASRKERAKSSPTFKDLDFQEMHFEGLHLDAETYHALMKTLQRDCRVLESFKIMDYSLLLGVHVLGKKMRERGGRGDSRRQGTQKVLYSTAMESIQGGGKPAEPLPDTDDETMGGIPAKHKDEKLLIFLGIIDILQSYRFIKKVEHSWKALVHDGDTVSVHRPNFYADRFLKFMGGTVFKKINPLRGASSRRKRSSIQPIRSVSQEVLATVKEETNEERKAQSLENLDDKDLGYSQKPDVIPSSTNLNVTASVATMSSVSSLDDIKLEHEEDVDSRGHRRTSSSTLALEDSAHPSTSPQSRTPDSELDVYLTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JWD1</accession>
    <name>A0AAR2JWD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PHKB</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Lipid-anchor</topology>
            <orientation evidence="1 7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037390445.1">
        <property type="nucleotide sequence ID" value="XM_037534548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JWD1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060624.1">
        <property type="protein sequence ID" value="ENSPNAP00000054547.1"/>
        <property type="gene ID" value="ENSPNAG00000021311.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439466"/>
    <dbReference type="GeneTree" id="ENSGT00950000183118"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF8">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19292">
        <property type="entry name" value="KPBB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="8">
        <location>
            <begin position="47"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphorylase b kinase regulatory subunit alpha/beta C-terminal" evidence="9">
        <location>
            <begin position="912"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1087" mass="124383" checksum="108F144185D3F5B2" modified="2024-11-27" version="1">MATLADVGWKLLEFKARSRRSGSIYEPLKLSILHHEDEPLWEKLDRSYNAVKSTILNYQSPTTGLFPVKTCSNYEEAKVRDSLYCAASAWALAMAYRRIDDDMGRTHELEHSAIKCMRGILYCYMRQSDKVERFKQNPSPHTCLHSVFNVHTGDEVHSHDQYSHLQIDAVSLFLLYLVEMICSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGMWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESRSHNTDAALLPCISYPAFAVDDDALYSQTLDKIVRKLKGKYGFKRFLRDGYRTANEDKNRRYYRPAEMKLFDGIECEFPIFFIYMIIDGVFRGNPAQVKEYQELLQDVVFQSYDGHAVIPKHYYVPADFVEAEQKKHGSQKRFPSNSGRDGMLFLWGQALYNIAKLLVDGLISPKDIDPIHRYVPRQDQRNVSMRYSNQGPIENDVVIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYRFLAVNKKLGLSGRPERPVGCIGTCKIYRILGKTVVCYPIVFDLSDFYLSQDVMLLIDDIKNALQFIKQCWKMPGRPLFLVLIREDNIKGSRFNPVLDMLASFKKGSIGGVKVHVDRLQTLISGAVVEQLDFLRVNEAEIPEFKSFEELELPRHSKVKRQTSTPNASDLEQQPEINIQEWYNRPTYEILQKFNDCGCLASQAQLASILLKKEGPDFFSRDESLKDELERIYRQAGSRKLWLAVRYAAAATQKFAGSIAPHITTLLVHGKQVTLGLFGHEEAVISNPLSPGVIKDIIYSKCSPHGEREAVLQQELVIHIGWIISNNPELFSGMLKIRIGWIVQAMKHELEIRAGDMRPQDIYQLSPSDVKQLLLDVLQPQQTGRSWLNRRQIDGSLNRTPLGFYDRVWQILERTRNGIMVAGCHLPQQPTLSDMTMYEMNFSLLVEDTLKNIVLPEYRQIIVELLMVVSIVLERNPELEFQESVDLDRLVKDAFADFQKDRGRPDRAEKQEDMEVFYNTPALGKKGTSSYLTKVVMIQLLQGEVKPSKDDPCSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JXP9</accession>
    <name>A0AAR2JXP9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calx-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FRAS1 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000063046.1">
        <property type="protein sequence ID" value="ENSPNAP00000054641.1"/>
        <property type="gene ID" value="ENSPNAG00000016732.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155313"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039005">
        <property type="entry name" value="CSPG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045658">
        <property type="entry name" value="FRAS1-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051561">
        <property type="entry name" value="FRAS1_ECM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739:SF4">
        <property type="entry name" value="FRAS1-RELATED EXTRACELLULAR MATRIX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16184">
        <property type="entry name" value="Cadherin_3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19309">
        <property type="entry name" value="Frem_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51854">
        <property type="entry name" value="CSPG"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043400602" description="Calx-beta domain-containing protein" evidence="10">
        <location>
            <begin position="38"/>
            <end position="3131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="3076"/>
            <end position="3097"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="290"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="409"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="518"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="655"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="782"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="899"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="1020"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="1143"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="1257"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="1374"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="1486"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="8">
        <location>
            <begin position="1609"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="11">
        <location>
            <begin position="1713"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="11">
        <location>
            <begin position="1825"/>
            <end position="1939"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="11">
        <location>
            <begin position="1954"/>
            <end position="2060"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="11">
        <location>
            <begin position="2075"/>
            <end position="2178"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="11">
        <location>
            <begin position="2196"/>
            <end position="2300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="3131" mass="346441" checksum="2922BE3E2DC38003" modified="2024-11-27" version="1">MTTLHFQCTATRWTAAAGAMELLPGLFLLASLTACASQRTGGHGLSYAYKPELSEDAILVANNGIRVPFGRSVFIDPVNDLVTEVQPGDRCSVTVLDSDPLSQRPGRLSPKKFPCEFGPNDVKYSHFGSRSPAEDRVRLQLRYDTQTETIIIPFMLEVEVLFTQLEIVTKNMPLIVERLHSSSSPIDRKVLEFSYDGTSERCEVASLASASALPRYGEVADGGKLENMMDCDAFLRAGIRYVHTSADKSPDRDYVPMVVELRNKENNVLKQERFHIMVRIKGGQKNSPPKPSFVAMMMMEVDQFVMTALTPDMLAAEDKETDPGDLIFNLTSPLSFEEGYMVSTDDRNLPITSFYQRDLKELKIAYTPPSLDSDTERIFQIELEVLDADGGVSDPFAFMIVVKPMNTLAPVVTKNTGQLLYEGQSRPLFTQNNLEVSDEDDLDSVRITVVDGLRHGELTVLGSSRKYFTPADLQVGIVVYQHDGSDTYSDNIIFRMTDGKNEVEFLFPITVVPTDDEPPIINANTGLVLFKNQMMPISPLMLSAVDIDSEDSTVKFTLEPPLSAIGEVLLRQSEAPEDPSSWKFSTEDGMYEKAVVEWIQKDITDGKLFYRHKGPHITETATDQFLFKAQDDADPPNQSDENMFFIRILPIDDVPPVLFPGTTLQMTVHEYQLTHFSKSVLSYTDLDSEDRDLKYTVTQPPTDTDENNPVRFGSIVLTEHPNTEVSEFTQAQVNHHKISYSPPDIELGIAAHVVQFRFTVEDTAGNSVEGIFTIFLQPVNNKPPQITNTGFTVFERGMHIITNSELDSTDLDTDSKQISFTLRQAPQHGQLQLNFIDLIKGNSFSLLDIYEGRLSYAHNGDESASDFFKLDVTDGVHVVPITVRVVVKPVDDEIPSLSLPVGTIGSHIDVLENGATEITTSVIQGKDEDTDDLRLTFIVENAPTFGEILVNGKLSSRFTQAEIISGLVVYAHTSGEIGLSSKQDSFNLTLTDMSDEWVVGGNKVSGVRVHVSILPVDSQSPVVTVGPTFSVMEGDKNTIGGHQIMVKDEDTLTEDILCTIIVQPTSGYVENISPAPGSEKSRSGTAVSAFTISDVNDGHVRYVQSIHKGVEPVEDRFTFRCSDGVNFSERQFFPIVIIPTNDEKPEIFSREFVVMEGMNVIIDTPILNGADADIPAEDLTFIISEPPKHGFILNQLTAASIPITNFTLEQIKEASSIIYEHDDSETTEDKFTVILTDGKHSVEGTVMVMIIPVDDETPRLAINDGLEIEIGETKEINSKILKATDLDSDDNSLTFIIRYGPGQGFLQRMTELGYIENITMGMNFTQIELNQGRILYTHTGQEGVRDLVKFDVTDGTNPLIDRYFYITVSTIDMVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPVRGHLESTDTPGMPIVSFTQLQLAGSKIYYIHTSDDEIKMDSFEFEVTDGYNPVFRTFRVSIVDVDNKKPVLTVHELLVTEGQTKLITPFELTVEDQDTAENLLKFTVTQSPVRGKLLFNNSRPVTTFTKQDLNENLISYKHDGTESSQDSFSFTVTDGTHTDFYVFPDTVFETRRPQTMKITIVPLDNGVPQIVVNKGVSTLKVLPSGHLGYTISSKVLKAEDRDSSPQTLFLQIIAQPEHGYLINLDKGNNSISTFTQADIDDLKICYVLKDKENATSDIFHFSVEDSGGNKLKGQHFRLDWAWISLQNRSYVVDEQAGLLEVVLRRRGYLGETSFVGISTLDGSAEKEKDFRGKFQKQVQFNPGQTTATWRIKILSDGKYEQSETFNIVLSEPVMGALESPAVATVEIVDPGDESTVFIPQASFAVEEDVGELLVPVRRTGDVSEELMVLCYTVQGTAGAFWSTVPSTVLSYSDYISRPEEQTSMLSFDKGEREKFCRVVIIDDSLYEEEESFNVTLSMPLGGRLGSEYPSTRISVLPDLDDAPVFYFGDQEYHVDESVGHVEVKVWRSGTDLSKPATVTIRSRKTDPVSAEAGVDYVGISKNLDFAPGVTVQTVRVTILDDLGQPLLEGIERFELVLRMPSNGILGEPGRAIIFINDSVSDLPKVQFKQAVLVGEESEGRLTAVVQRSGDISHGSSVRCYTRQGSAQVASDFDERPNTDASIITFLPGETEKPCVLTLVDDLVYEEEEEEMKLVLGSAKSDSLYGASVGRQNETLIKIKDTADKPIIKFAETKFSVSEPKESGQVSVVRIPVRRVGDSSMVSVVRVHTKDGSALSGEDYHPISQDIEFKEGEREHVVEVEVLFDGVREMREAFTVHLKPDENMMAETQVTKAIVYIEETNSMADVTFPSLPHVVSLLHYDDVTRTKANVHPPAGYPVVCVTACNPKYPEYDKTGSICLSERINDTLTRYRWLVSAPSTPDGVTSPMKEVDFDTFFTSSKLITLDSIYFQAGSRVQCAARAVNSNGDEGLELSSPITSISTEEGMCQPRKVGTVGAEPFSAKLRYTGAEDPEHANLIKLGVTMPHIDGMLPVVSTRPLSNFELTLSPDGSRVGNHRCSNLLDFNEVSTRHGFLTKATRNMESVGETSPYQYSASLRGQGTLRFYRNLNLEACLWEFVTYYDMSELLSDCGGTIGTDGQVLNLVQSYVTLRVPLHVSYVFHSPVGAGGWQHFDLQSELRLTFVYDTAILWKDGIGSPPQAELQGALYPTSMRINEQGRLVVNFRTEARFRGLFVASHPASSLTSMVMSADHPGLTFNISLVRTEPTYNQPVQQWTFTSDFAVRDYSGTYTVKLIPCTTALNIEYSVPAVCNPREPITFDLDIRFQQVSDPVAVEFSLNTQMFLMSKRSVWLSDGSMGFGQESDAAFSEGDIIYGRVMVDPVQNLGDSFYCNIEKVYLCTGADGYVPKYNPTKFEFGCLADSPSLLYRFKMIVGDKAQPETQAQSFGDVGFNALLAVDDPSAISLVKQPGSDGFRLDSAPLFQVAAGREWYIHTIYTVRSRDNANRGIGKRSLEYHSFMAGATLPSAQSGRHGRRSARDVPAFAEEISAENNRGTNIMHIALDRRNRRAAPDGELFADSLAPQGLGSYSAEDKAVTVVGAVVGLLLAVLLMAVLVLVVRSQWRPTKQAPKVSSSMQPVILRSGFDCSDSSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K167</accession>
    <name>A0AAR2K167_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GRAM domain containing 1Bb</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K167"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054374.1">
        <property type="protein sequence ID" value="ENSPNAP00000057985.1"/>
        <property type="gene ID" value="ENSPNAG00000014961.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156649"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000008">
        <property type="entry name" value="GRAM domain containing 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF3">
        <property type="entry name" value="PROTEIN ASTER-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="764"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="514"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="188"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="475"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
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        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
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            <begin position="166"/>
            <end position="176"/>
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            <end position="427"/>
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        <location>
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            <end position="455"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="876" mass="100802" checksum="E131C49E475A2E8C" modified="2024-11-27" version="1">LPATMTDTLRPPSLQVHVPRDDPGPSDEGGGNGKSAPDSLAWSSSSTPTLRRRRFKMRRTKKVPSERERLENGLLRHPGFRSASKEYLQLPSIEITPSSDEDAPWSNCSTPSASPRRKRFLLRKWLKVRERKELCSESSSQQSSQQSSHDDDSTRFLTPLIREESTASNSNRSSPACSPVLRKRCRSPTVQSLEGENMVEKGSEQSDTSKSPSTPEQLVTRTYSVQSTRSSSKNSKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFRWETLLTVRLKDICSMTKEKTARLIPNAIQVCTDTEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKELWHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPAEENEVSNDNSSKSSGENKPDSSPPSHKRSITHSTLNSTTPPSSTDMPLSVKTDGLQSVTVELQSPVPSPSLDFNDNEDIPTELSDSSDTHDEVEVQAFHEDLNGRQYINEVYKFSVDKMYSFLFTESQFMTDFMEQRRFTDIVFHPWKKEEAGNQKREIMYTISLSNPLAPKTATVSETQTLYKASQESECYIIDAEVVTHDVPYHDYFYTLNRYTLTRVAKNKCRLRVSTELRYRKQPWGLVKGFIEKNFWSGLEENFRSLELELGKAEDSLLESHRPSPKAKALKSSTLRRRKRPLPHLRTPHLEETLSPVTTPTDEEVIQRIKHVAGSTQTRQSPEHSPAGGPFCSISKLLLIISFVLVLLVFLNMMLFYKLWMLEYSAQSLMTWQGLRLDKSKLPQTQLEWAQLLESQQRFHEAELQKWREIIKSSVLLLDQMKDSLLSLQRGVTLRDFGSDSDEKQSRYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K795</accession>
    <name>A0AAR2K795_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Neural cell adhesion molecule L1-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Close homolog of L1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">May interact with L1CAM. May interact with ITGB1/ITGA1 heterodimer and ITGB1/ITGA2 heterodimer as well as with ANK3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K795"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087172.1">
        <property type="protein sequence ID" value="ENSPNAP00000058364.1"/>
        <property type="gene ID" value="ENSPNAG00000004665.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165371"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000367">
        <property type="entry name" value="Neural cell adhesion molecule L1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000418">
        <property type="entry name" value="Neural cell adhesion molecule L1-like protein"/>
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    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026966">
        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF45">
        <property type="entry name" value="NEURAL CELL ADHESION MOLECULE L1-LIKE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="971"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1000"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1129"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="18"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="116"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="222"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="314"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="407"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="494"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="22">
        <location>
            <begin position="594"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="22">
        <location>
            <begin position="694"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="22">
        <location>
            <begin position="792"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="22">
        <location>
            <begin position="898"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="675"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1177"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1186"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1202"/>
            <end position="1224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00072847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1251" mass="139247" checksum="14C5DC65AED86DC7" modified="2024-11-27" version="1">MLHISDTVCSCSAVEQLPTITVHPPKSLIALPFDESITLTCEAKGNPKPVFRWTKNGQDFYPHLENKLIKFENSGSFVIPNNKGLAKYQGKYRCYASNKLGTAVSEETDFTVPTPPKFPKERIEPVVVKEGDSVILECNPPKGTPPVQLYWMTMGLQHIEQDERVSMGLNGNLYFSNTLVKDSRSDYCCFASFSAIRTIVQKTAMTHVVTLPCVASGLERKPNLLVPSGDQSQINLVTGEELELECISEGFPTPKIEWTKLGEKLPKRSNIKNYGKLLTISKVTEEDSGKYMCKAKNSVGETVHYFHVEVEEPPRWIEEPVRTQVVTIGSDVHIKCHAAGKPPPAITWLRDGKPLHGEWTPTQSSDLGDTLILHKALSNDTGVYQCEASNTHGTVLATANVMVMNHPPLILTRDYLEYATVRGKSVTMDCRVFGSPPATVHWSGSVEEERFSIHENGSLQIHTAEVEDMGKYMCYATNSQGTASISAELYVKEPTRIVVPPQDMEVRRGSTAELECQADMDQSLREDLEILWRKDGIDIYSNFTDDSGFYIDERILYIFNANHSDGGLYTCIARTPLDEDTAAARVTVLDVPDPPVRLFLSDLRDRSVRLHWVPTKDHNSPVKEHIIEYEENHWDPGKWKPLLRVSGNQNSAPLSLYGHINYQFRVTAVNAIGAGRPSKPSERYKTPPAAPDRNPENIKIEGHVPHQMHITWKPLLPIEHNGPGLEYKLSYRQLGVEDTWTEHTMERHLFVVKDTPTFVPYEIKIQARNHLGWAPEPRVVTGYSGEDLPLAAPEDVSVVVLNSTLLRVSWSPIPQAQLRGHLGGYSVSVHWWRTRSLLTSKEVPAEKQFLTLTGNRSHTMVPGLKPFSEYRLTVNVFNRRGNGPSSSPLTFTTPQGVPERPPILRATNPQGDSITLVWAPPLEANGILTGYRLQYQLINETMEVDEAHTVNISGPDTTQLVLPNLKLHSQYQFYLSACTVVGCGPAVSEEEGTVRTAGDGLANVLVLVCLSFCLFFGLSLSLLFSALFNISTTVSHSFANISWTAKGRQSDSEIYVAIMDHSDGIWRISEAVNTSQSFHVIEGLEPETVYTVRLLASRWPDNSSLFEEVFTTRGKGMAGVSESISSRGWFVGMMCAVALLTLAVLIACFVTRNKGGKYAVKEKEDVRMDLEAHCVQEETSGEYSDSDEKPLKRSKRLLEGDVSGDDTSVDSSVDYEDEDSEFNEDGSFIGEYAGHKRKLSAEVNGHTTMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K9M7</accession>
    <name>A0AAR2K9M7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aquaporin 8a, tandem duplicate 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K9M7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067650.1">
        <property type="protein sequence ID" value="ENSPNAP00000061013.1"/>
        <property type="gene ID" value="ENSPNAG00000010931.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159304"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000019">
        <property type="entry name" value="AQuaPorin or aquaglyceroporin related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023277">
        <property type="entry name" value="Aquaporin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF34">
        <property type="entry name" value="AQUAPORIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02020">
        <property type="entry name" value="AQUAPORIN8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="27643" checksum="241F65D2F943DA78" modified="2024-11-27" version="1">MTTTESKSELFTIASVESAQSQKEQASSMGFFERYVQPCLAELLGSTLFIFVGCVSVISNIGVTGSIQPALAHGLALAITIAVFGEISGGHYNPAVSVCVYLLGGMEVVLLGPYVLAQMFGGMIGAGLAKAISPSAYFYNASGAAFNAVKSSGDIGPATVAEIVMTLFLTMVVSMSAVNGRTKSQLAPFCIGLTVTANILAGGMISGACMNPARAFGPAAVAGHWDSHWVYWVGPLAGALLTASIVRYFVIYSHLFRFANVSADVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K9P9</accession>
    <name>A0AAR2K9P9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">EVA1A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K9P9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083846.1">
        <property type="protein sequence ID" value="ENSPNAP00000059214.1"/>
        <property type="gene ID" value="ENSPNAG00000037404.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178339"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF8">
        <property type="entry name" value="RERATING FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="29914" checksum="B2A133E14D32FFC1" modified="2024-11-27" version="1">MYLCVFRESHCRQQILQTDCSVSGAAVCPPADGHHSTVDQTLQLNDVKRAVTDQPDHRERPVTDQLHQPDHRERPVTDQLHQPDHRERPVTDQLQTNLTTERDQLQTSYTNLTIERDQLQTERDALQKRLSELEKHINNPEWRYFNYSVYYISTETKAWSESREDCRERGADLLIINSREEQVRERDRERERERERERERERERERERERERERERERERETERERQRERERERERERERMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KFL3</accession>
    <name>A0AAR2KFL3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ras-associating domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RASSF10</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KFL3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052151.1">
        <property type="protein sequence ID" value="ENSPNAP00000063070.1"/>
        <property type="gene ID" value="ENSPNAG00000035678.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182839"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033593">
        <property type="entry name" value="N-RASSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048945">
        <property type="entry name" value="RASSF8/10_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286:SF13">
        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21712">
        <property type="entry name" value="RASSF8-10_RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="36"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="409" mass="46186" checksum="19FFA3AE735574D2" modified="2024-11-27" version="1">MWKGRGLRSREASPMEHEDCKVSVWVCREEKLVSGLSRRTTCGDVVRALLEDQRSAVSARSYCIVEKWRGYERALPARTRLLRLWSAWGRERENVRFVLVRSDASVPSGGARSAEARVVSSAESRERAPERESAPAVTCSRERQRRVVRKAFRKLERMKQSRGGGGEEEEEEEEGKMETLVHLVVSQDHTIRQQLERLSELDGEIERYEAKVHLDRIRIHGVNYVQDTYLAESSPERDEEEAAAAEEERVERREELKEPKDKDAGIVTEEAPASPASVPQAETLSDNELQLEEERIKTQLDTSLYIGLRLNTDLEAVRSDLDMSRELWEAKDKELRDLLEKVHSLNEGAEEEVDAPVVQSAAEKSSGWVEEARGLSKSCGGGNDDDSDTGLSSMHSQDSDNPPVCESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KG73</accession>
    <name>A0AAR2KG73_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">RBM34</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KG73"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079114.1">
        <property type="protein sequence ID" value="ENSPNAP00000061432.1"/>
        <property type="gene ID" value="ENSPNAG00000031184.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000276864"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.150.10.10">
        <property type="entry name" value="Serralysin-like metalloprotease, C-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011049">
        <property type="entry name" value="Serralysin-like_metalloprot_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="466" mass="50626" checksum="672CAC30DECAF63D" modified="2025-10-08" version="2">MFGAVAMVTLSDGAAQWGKKCMNSDLSQKFKVGASNKVASEQKFKVGVSNKVACEQKFKVGVSNKVASERKFKVGASNKVASEQKFKLGVSNKVASERKFKVGASNKVASEQKFKVGVSNKVASEQKFKVGVSNKVASEQKFKVGASNKVASEQKFKVGVSNKVASEQKFKVGVSNKVASERKFKVGASNKVASEQKFKFKVGASNKVASERKFKVGASNKVASEQKFKVGVSNKVASEQKFKVGASNKVASEQKFKVGVSNKVASERKFKVGASNKVASEWKFKVGVSNKVASEQKFKVGVSNKVAREWKFKVGVSNKVASEQKFKVGASNKVASERKFKVGASNKVASEQKFKVGVSNKVASEQKFKVGVSNKVASEQKFKVGVSNKVAREWKFKVGASNKVASERKDKVGVSIKVASEWKDKVGNLFFFSLSLSLSLSLSLSLSLFPHLPLHVWIQYYLFELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KGV0</accession>
    <name>A0AAR2KGV0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KGV0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081615.1">
        <property type="protein sequence ID" value="ENSPNAP00000061694.1"/>
        <property type="gene ID" value="ENSPNAG00000037809.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166507"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF28">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER C ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="147"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="258" mass="29478" checksum="34E5E2FCE3C211F5" modified="2024-11-27" version="1">MHRLRWTVEFQKQAGELGKQRVLALMLNYLSPFFSFIVFSCLQEDVYRKVRLYRRLCVALLIVCVVLLAVLLALAVKLSEAQSSQRYQPAAESSTAPASCSLQTCEALFPTLMASHHSGEAGEPQHVVADNHAGLVCSECGKGWLKYDDSCYLLSRVRRTWQESREECLKLGGDLTVIRSERVQRFLTNKGLMQYWIGLHRSETQEWTWINNTALTTGYWATNLQEGNCVFLNGGKWHKGNWHSSRCELYSNYICQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJ75</accession>
    <name>A0AAR2KJ75_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KJ75"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052479.1">
        <property type="protein sequence ID" value="ENSPNAP00000064350.1"/>
        <property type="gene ID" value="ENSPNAG00000034720.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000275439"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="268" mass="29509" checksum="D7418C19AAFB103D" modified="2024-11-27" version="1">MNKPYISNNIIGVGVAIPNVYSLSEREALVPQGLQVLSVVVVGGTQMDAVMSLCGFNGLERERLMSGSTRPRIKQNLVRNMLGDKERDVLGDAEGDVLGIAERDVLSDERDDLGDERDELGVAERDVLSNERDVLGVAERDVLGVAERDVLSEDERDELGDDERDELGDERDELGVAEREVLSDERDDLGDERDELGVAERDELGVAERDELGVAERDELGVAERDELGVAERDELGVAERDVLGVAERDVLGVAERDVLSDDERRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKW6</accession>
    <name>A0AAR2KKW6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrosomal protein 68</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CEP68</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKW6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056250.1">
        <property type="protein sequence ID" value="ENSPNAP00000062766.1"/>
        <property type="gene ID" value="ENSPNAG00000013870.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00810000125473"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="453"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="760" mass="85618" checksum="3790CFC1A7D6D593" modified="2024-11-27" version="1">MAMGVDRAFLDTSSQMEHRNYGRWKSRIPGFIQSDHTRSLTKETGDESRPSHGLNAKDLDRERTASKKTVTMAPTSRYMTSRRLYTARKPLITAERHISILKNPLTQECSEIAHMSSVANHQKDMEAMVSKQLDTYTTENISIPQVIASSPSASREDLYASLSLSDLRLSSHEEPDFSSVPSSSKNERRSFSSPPLDDLSLTVPLSPKWSSTQRTFSHSYTSQTRPSRKTKTRVFEQDKDSVFKTGSSRSYNQEYSSRLKPVSMYQANYWACAIPSSLPPSPDRRSPSWDPDKEYQALLDYTYPLRPNMGNNWSSNESRSLLRTDPLLEDSGIELDRFCSSSSLSYLDQSLTGIRHARSGPTSGQRLSGLQSLNPRELSRSKSSDGRLSSSLYSSLDQVGLSVESLDGEGKQNFHCRKFGVFSTSRTVPFIRSTCILPQPGSLGELDEEFLRLPEQLQELQDLSHQLRDITAQMAQPINTSWESLEKESASGAYSAAQMEQNAAEVSAMQEEANEMDEMQHRSSAEVPLQREKFSEQLKGLGARVQIISSEVDKGSLREVEAIIDQLSGTTLFEFQRRTGTNLAEEETKESVLQHIQAFCSNLEELIQWLYKVVEKMEVLSPPSVDIESVKASLAHYKVSDVNKIQNMILNRFLRQVTYVSLATINDCNYVFQSFQKEIQAHQPLTASVLQTGELLLCCMNSASPYLKETLKLIERQSCVLETHSEHLFSSILSAMDCLTEPISQDDTGKHYMPILHILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KLR1</accession>
    <name>A0AAR2KLR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Discs, large homolog 4a (Drosophila)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KLR1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083086.1">
        <property type="protein sequence ID" value="ENSPNAP00000063352.1"/>
        <property type="gene ID" value="ENSPNAG00000021431.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157956"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097113">
        <property type="term" value="P:AMPA glutamate receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001402">
        <property type="entry name" value="Discs, large homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000049">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019590">
        <property type="entry name" value="DLG1_PEST_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF33">
        <property type="entry name" value="DISKS LARGE HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10608">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01277">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="128"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="223"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="429"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="543"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="8">
        <location>
            <begin position="668"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="854" mass="95282" checksum="68119B547887EE0D" modified="2024-11-27" version="1">MFPLTRGPPGLHHCLRFGPRPPAGSHSHHWGPEGRVHETGEDDFYCRPFSESFWAVYLIMKLKYRYQDEETPPLEHSPAHLMAGKSGELLHLSDASHTPMDGVHGYAPHTHISPAKMNGLKGEVEYEEITLERGNSGLGFSIAGGTDNPHVGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNDADVREVTHSQAVDALKEAGPIVRLYILRRKPAAEKLTEIKLIKGPKGLGFSIAGGVGNQHVPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNNMSLEDVMHEDAVAALKNTAEVVYLRVAKAPNLYPQDIYSPPDITSSYSPHMDDMGHGYLSDYPQTLTPTSPRRYSPIPKGMLLGDEDFPRLVFPITNLYRPLGIDTDHYNHYTLSLSLSVSLSLSLSLSVSLSLSRLSLSLSLSLYPRRVLIHRGLSGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRHATHEQAAAALKNAGQTVTIIAQYRPEEYSRFEAKIHDLREQLMNSSMGSGTTSLRTSKRSFYIRALFDYDKTADGGYLSQAVSFRFGDVLHVLDCSDEEWWQARRLSPHGDLEEAGYIPSKRRVERKEWSRLRSKGRDSIGKSVLLNTVDLLTESLCGWSHAHIRSSEPKLDLSNHHRYMHYARPIIILGPMKDRVNDDLLSEFPDKFGSCVPHTTRPKRDYEMDGRDYHFVSSREQMEKDIQSHRFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAQLHPIAIFIRPTSLDINKRLTEEQARRALDRAIKLEQDFIECFTGIVEGDSFEEIYHRIKSVIEEQSGPYIWIPARERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KLS2</accession>
    <name>A0AAR2KLS2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin--tyrosine ligase</fullName>
            <ecNumber evidence="11">6.3.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TTL</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + L-tyrosine + ATP = C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-L-tyrosyl-[tubulin] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17605"/>
            <dbReference type="Rhea" id="RHEA-COMP:16434"/>
            <dbReference type="Rhea" id="RHEA-COMP:16435"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:149554"/>
            <dbReference type="ChEBI" id="CHEBI:149555"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.25" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017580549.1">
        <property type="nucleotide sequence ID" value="XM_017725060.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KLS2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043839.1">
        <property type="protein sequence ID" value="ENSPNAP00000063056.1"/>
        <property type="gene ID" value="ENSPNAG00000005397.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444082"/>
    <dbReference type="CTD" id="150465"/>
    <dbReference type="GeneTree" id="ENSGT00940000161907"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004835">
        <property type="term" value="F:tubulin-tyrosine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052492">
        <property type="entry name" value="Tubulin-tyrosine_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46570">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46570:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="406" mass="46139" checksum="A102F8EE3D934440" modified="2024-11-27" version="1">MNNVTPMYTFVLRDDNSSVYAEVSKILIATGKWKRLKKDNPRFNLMLGERNRLPFGRLGHEPGLMQLVNYYRGADKLCRKASLVKLIKTSPELKDSCNWFPESYIIYPTNLNTPVAPATNGISHLKNNPKTDEREVFLASYNTKKENGEGTVWIAKSSAGAKGAGILISHDATQLLEFIDNQGQVHVIQKYLERPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTSSEPYNSSDLQDMTSHLTNHCIQKEHSQNYGRYEEGNEMFFDEFRQYLLNVHNVTMETSILPQIKQIIRSCLMCIEPAISTKHLSYQSFQLFGFDFMLDENFKVWLIEINGAPACAQKLYPELCQGIVDVAISTVFSLNADALSSSPPFSTSPSLSSSSCSSPKLRAPQQFGPFIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KM26</accession>
    <name>A0AAR2KM26_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Theg spermatid protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">THEG</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017552549.1">
        <property type="nucleotide sequence ID" value="XM_017697060.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552551.1">
        <property type="nucleotide sequence ID" value="XM_017697062.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037387102.1">
        <property type="nucleotide sequence ID" value="XM_037531205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KM26"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067322.1">
        <property type="protein sequence ID" value="ENSPNAP00000063166.1"/>
        <property type="gene ID" value="ENSPNAG00000033478.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108427117"/>
    <dbReference type="CTD" id="51298"/>
    <dbReference type="GeneTree" id="ENSGT00940000154630"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042401">
        <property type="entry name" value="SPMAP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006623">
        <property type="entry name" value="THEG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15901">
        <property type="entry name" value="TESTICULAR HAPLOID EXPRESSED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15901:SF16">
        <property type="entry name" value="TESTICULAR HAPLOID EXPRESSED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14912">
        <property type="entry name" value="THEG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00705">
        <property type="entry name" value="THEG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26641" checksum="DFD7DF3F6782BCDE" modified="2024-11-27" version="1">MATRIDQLARPKPNLLKFPDRRSVYWLDELPPSLNSSSTAFDLTPRWSKLAESKKLIPNFEDNRQSPGWKLSAAVLKARPSDRVCALALPRMPAAGWQPDRPLLPTVSVAVRTAKANAWICQLARPRKMCAAMTQFCSSEISPSVPSLAKPSPRTHLLATPKQDHPQYCPDRPVSWPVAKPALEAVASERLQVLATPRLRKPLFEGFDPYRVSSAARAATASPRLQELALPPARRCRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNQ3</accession>
    <name>A0AAR2KNQ3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNQ3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056620.1">
        <property type="protein sequence ID" value="ENSPNAP00000063746.1"/>
        <property type="gene ID" value="ENSPNAG00000033831.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204390"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF62">
        <property type="entry name" value="INTERFERON PHI 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00076">
        <property type="entry name" value="IFabd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2 6" id="KW-0202">Cytokine</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043636065" evidence="7">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="186" mass="21517" checksum="3E75B9F5A52AF8C6" modified="2024-11-27" version="1">MEFYVCLSWISILLCIVQVTSMPTKCQLQRTLMAKTHNLLETMTGPFPLQCLDDIPVEIKSPTWALQSNNSKQDVGAAKAVYKSLMKVSSLMESDSFPQSWDADKLVKFQIIMDRQIEESECITRIKTQKEEDDFAVRDAALTDYFDKLEKLLKEKEFSVCAWEVVRREVLSVLQFNLVENSNNFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNS6</accession>
    <name>A0AAR2KNS6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Centaurin-gamma-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">AGAP1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the centaurin gamma-like family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570429.2">
        <property type="nucleotide sequence ID" value="XM_017714940.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNS6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077251.1">
        <property type="protein sequence ID" value="ENSPNAP00000064067.1"/>
        <property type="gene ID" value="ENSPNAG00000009031.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437681"/>
    <dbReference type="GeneTree" id="ENSGT00940000154793"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08836">
        <property type="entry name" value="ArfGap_AGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04103">
        <property type="entry name" value="Centaurin_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01250">
        <property type="entry name" value="PH_AGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000001">
        <property type="entry name" value="Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000027">
        <property type="entry name" value="Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000178">
        <property type="entry name" value="Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000332">
        <property type="entry name" value="arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000199">
        <property type="entry name" value="Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051282">
        <property type="entry name" value="Arf-GAP_GTPase_ANK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45819:SF1">
        <property type="entry name" value="ARF-GAP WITH GTPASE, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45819">
        <property type="entry name" value="CENTAURIN-GAMMA-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="728"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="20">
        <location>
            <begin position="1015"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="1174"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="161"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="649"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="877"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="657"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="704"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="886"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="906"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1265" mass="138898" checksum="D16A2D8380BF199A" modified="2024-11-27" version="1">MHLLDGNYPERMERGTPQRKTVYRISLTLVKKESLQGGGGVATASRRLENPKVSAYRREGSSEIQRSCPLEEVKEVEDETDDFAPHAYRNFRTFSTGQLELGRLKVSRKMRHLQKDNSLELDETHSSASPADAYCKEGTENCGALHKANVQAPDMEADRFKADGETEESCKAAAGNGESAGIKRKSMLKTSRSAEEQVSGRTVEDQVSEEEKDSQKGGKLPKDSSLSSLLAKLNGKAAKQAPQTPPVKRHPSLLRRSFSFRHWTGSELLRIRALSKEKHHSSSGCIGHEEAQVKSQSAVVLQNPAFDEAEPAEKGEKRNTLDVGEVLVSRRERAKGKNRTLDNSDLLRLSDKSLAEKEGFIRGSGSRSSGQERRLIRFFSGIFTKKDGTSTPVSSPSNRALRKNGLLGSQRRATLGYSQSSTESVNGFLQDDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEADVDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFALWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTADVPLVLVGTQDAISAANPRVIDDARARKLSNELKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAPVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPQTANTPTPVRKQSKRRSNLFTSRKGSEPDKEKKGLEGRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRAISTCAAPGPKTNGLTKDMSNLQLGQAPGSVSSSSSASQMASGISLVSFNSRPDGMHQRSYSVSSADQWSEATVIANSGVSTDTGLGDSVCSSPSISSTTSPKMDPPPSPHANRKKHRRKKSTSNFKADGFSGTAEAKRKAWKLNRVGSLRNIYNSSNTEEQEENFEFIIVSLTGQTWHFEATSYEERDAWVQAIESQILASLQSCESSKQKSRLTSQSEAIALQSVRNMRGNSRCVDCEAQNPDWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDEWPLELIKVMSAIGNELANSVWEANAQGRLKPAPDASREERERWIRAKYEQKLFVASLLCTELPLGQQLLRSTAEEDLRAVVLLLAHGSREEVNETCGEGDGRTALHLACRKGNVVLTQLLIWYGVDIMARDAHGNTALAYARQANSQECVDILLQYGCPDERYPLIATPNLSRRNNNRNNSCSSTGSSALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KPU6</accession>
    <name>A0AAR2KPU6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Epidermal growth factor receptor pathway substrate 15 like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">EPS15L1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KPU6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081506.1">
        <property type="protein sequence ID" value="ENSPNAP00000066348.1"/>
        <property type="gene ID" value="ENSPNAG00000005719.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155438"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF69">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="13"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="127"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="288"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="289"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="327"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="646"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="744"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="685"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="763"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="877" mass="96259" checksum="CFB524D48D264A79" modified="2024-11-27" version="1">MAALMSLAQISSGNPAYENFYRQLDPANTGKIGAVEAAQFLKKSGLSDSTLSKIWDLSDSERKGFLDKRGFFIALRLVASAQSGNDVSQSSLNQTISIPTPKFRDTASPSLVSTASSDATWAVKFDDKVKYDGIFESLLPVGGLLSGDKVKPVLINSNLPLDVLGKIWNLSDVDKDGHLDKEEFSVAMHLVYRAREKEPVPSTLPSSLIPPSKLKKTGVALPGSVPVLPSSPFLMKDALRSTVPLSKSPLTVASNLSPSNSFRSSRPSPVPSPAPLQPVSLNWVVPQEDREQYEEIFELADSDFDGMVGGGEVKDIFINSGLPQGVLAHIWSLADTKCTGKLTKEQFCLAMHLIQQKVKGVDPPQNLTPEMIPPSERGSTSSTTISSDFSSSVGFELAGLSMPSISRDSSSTLAPVELTGIKELDDISQEISRLQSEKRSIEQEIRQKEEVIRQKNNEVHGMQGELEHENSSLQDLEQQKQVAQDRLQEMEQQRSKLDGMEKDAKSKCTEETSRISSLQTQILSQESDLKTQEKELSRTKTDLYCLEQEENQLEESLRAGKAKLESILKLLKTSQDEMDETRSEISQIQESQKALNKSIERFTKALNGNMSNLSEIDQLLTEESSIPSTTVEDSFKSKLAMFNTNPTQDLSKDPFQSEDPFRSDPFNKADPFGSDPFKQDDPFRDPFSSSDPFAKSAASPLKASPFGRQRSVTSSSSKFKDSDPFATADPFGYDSFGGKTGFADFGNMSKGSSGQPLGRKPTYPLPPPKKPVPARPAPPPYGTSSAGAHKGLAAHSQVFTAPHVRKPHDSSLSNRSAVTASSAFADFRAFGSEVEQLEWAKRESKKEEEERLRRLRLQEQQDLELALALSRADMSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KQG5</accession>
    <name>A0AAR2KQG5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KQG5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046020.1">
        <property type="protein sequence ID" value="ENSPNAP00000066568.1"/>
        <property type="gene ID" value="ENSPNAG00000034334.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164160"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF6">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="463" mass="49118" checksum="A17D3DB599423669" modified="2024-11-27" version="1">SRSLADGLHQAPGRTEQDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTDQDRGFNGAEGTDQDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTDQDRGFNGAEGTEQDRGFNGAEGTEKDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEKDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEKDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTEKDRGFNGAEGTEQDRGFNGAEGTEQDRGFNGAEGTDQDRGFNGAEGTDQDRGFNGAEGTEKDRGFNGAEGTEQDRGFNGAEGTDQDRGFNGAEGTEQDRGFNGDRREKRNTAVQLISGERANLTLSSSTLAMLLLSMRSFSMEMGSLLLRTSWISSRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KR01</accession>
    <name>A0AAR2KR01_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KR01"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057276.1">
        <property type="protein sequence ID" value="ENSPNAP00000064546.1"/>
        <property type="gene ID" value="ENSPNAG00000031905.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230358"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001648">
        <property type="entry name" value="Immunoglobulin heavy variable 4-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="101" mass="11037" checksum="94C082BD6B8F46BC" modified="2024-11-27" version="1">CNLTQPASMVINPGQSMSLTCKVSGYSVTDSSYCTDWIRQPAGKALEWIGEICGDGRTYYSDKLKSRFAVSRDTSSNTVTLRGQNMQTGDTAVYYCARGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KR48</accession>
    <name>A0AAR2KR48_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563015.1">
        <property type="nucleotide sequence ID" value="XM_017707526.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KR48"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071778.1">
        <property type="protein sequence ID" value="ENSPNAP00000066803.1"/>
        <property type="gene ID" value="ENSPNAG00000032606.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433148"/>
    <dbReference type="GeneTree" id="ENSGT00390000012918"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17407">
        <property type="entry name" value="MFS_MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF5">
        <property type="entry name" value="UNC93-LIKE PROTEIN MFSD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="49388" checksum="FCD06DAB1F6D46A9" modified="2024-11-27" version="1">MADFRTYNVVILGIGFLFIFTAFTTCGNIEQTVVKSLHNATFSGSGYHSLGIIYGIFSFANLLAPTAVAVIGPRFTMFFSGILYSGYIAVFISPSSWSFYLTSVLIGFGAALLWTAQGHFLVENSDASTINRNTGMFWALLQCSMLFGNLYVYLDWKGKTEISDRDRRIMFIALLITSVLGTLSFLMLRKSGQQEEEVLTEEEGQSLLSSRQIYKQRANSAIKNAKTEFKTILHLFGTKTILLLSFCMAYSGLELCFYSGVYGTCIGATTQFGDTSKALIGISGIVVGFGEIVGGGLFGLVLKNNRFRRTSVVFLGMVVHFVAFYLIFLNIPDDAPVVFKTSIQHKPYLSPSVSIALLCSFLLGLGDSCFNTQLYSILGRVYSEQSAPAFAIFKFVQSVFAAVAFFYSGYLLLTWQLLIMVILGFTGTLCFFVVERMQNVSADLPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L318</accession>
    <name>A0AAR2L318_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL4m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">39S ribosomal protein L4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRPL4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L318"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055802.1">
        <property type="protein sequence ID" value="ENSPNAP00000069109.1"/>
        <property type="gene ID" value="ENSPNAG00000034956.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014512"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000005">
        <property type="entry name" value="39S ribosomal protein L4, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_uL4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF6">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="340" mass="37871" checksum="3B0F02AA275E8286" modified="2024-11-27" version="1">MRPDVTCSGRRSASARPSRWSVSDAACGVYILSISLICIMFRATLTVCGGGIAKRLFSSFSGESALPSNLRLPPNLIGVPREKRAFPASTDLPVLRRCEADIPAHLAPVQTWLQSLESRHSEPLGVVDLHPDVFSVPVRLDILHAVEVWQRNFKRISYAKVKSRAEVSGGGRKPWKQKGSGRARHGSIRSPIWRGGGVAHGPRGPTSYYYMLPMKVRVQGLKIALTSKLAQDYLHIVDSLEIPTPDPHYLQDLAKHRQWGDSVLIVDVGEEFPQNILQATESLKTINIIPAIGLNVHSMLKHESLVLTLEAVKFLEKKLLWHDTRFTPLYPFKLPYSDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L8W7</accession>
    <name>A0AAR2L8W7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein axonemal assembly factor 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">WD repeat-containing protein 92</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Key assembly factor specifically required for the stability of axonemal dynein heavy chains in cytoplasm.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L8W7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079500.1">
        <property type="protein sequence ID" value="ENSPNAP00000072985.1"/>
        <property type="gene ID" value="ENSPNAG00000031169.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183091"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000047">
        <property type="entry name" value="Calcineurin subunit B type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000258">
        <property type="entry name" value="WD repeat-containing protein 92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 13" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="146"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="598" mass="66445" checksum="5434F365C00BCC16" modified="2024-11-27" version="1">IHTDAQTHLCVTYEGKDWIWAIFTCCVNKAICFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVSVSCFVLPAQSESPPAVQTTLHLHLWHLADALIQSDLQFDHFTQVGQGGVRSLAQGSLLISRFVTSMSALLEKPQIIAHVQKSLNYTVFDCRWIPCSAKFVCLGNFARGTGVLQIYEIQHGDAQLVKEIEKPKPIKCGTFGATSLQQRHLATGDFGGNLHVWNLEAPEVPVYSVKAHKEIVNCIDGVGGLGIGGGAPEIVTGSRDGTVKVWDPRQKDTPVANMEPAEGESKRDCWTVAFGHAFNDQDRCVCAGYDNGDVKLFDLRNMSVRWENNIKNGVCSVEFDRKDISMNKLVATSLEGNFHVFDMRTQHPTKGFASVSEKAHKSTVWQVRHLPQNRDVFMTAGGAGSLYLWKYEYPAKRSEKDGDGVDGGVAGTLNLLQNVNLSTQPIASLDWSPDKQGLCVCSSFDQSVRVLIVTKLNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LA48</accession>
    <name>A0AAR2LA48_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peroxiredoxin-like 2A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peroxiredoxin-like 2 activated in M-CSF stimulated monocytes</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Redox-regulatory protein FAM213A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peroxiredoxin-like PRXL2 family. PRXL2A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LA48"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072684.1">
        <property type="protein sequence ID" value="ENSPNAP00000071522.1"/>
        <property type="gene ID" value="ENSPNAG00000001154.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165336"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032801">
        <property type="entry name" value="PXL2A/B/C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28630:SF31">
        <property type="entry name" value="PEROXIREDOXIN-LIKE 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13911">
        <property type="entry name" value="AhpC-TSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="135" mass="15084" checksum="C1400B91543673A0" modified="2024-11-27" version="1">GCRTNEASELSSLRPQLDAFGVPLYAVVKENIGTEIQNFRAYFNGEIFLDEKKCFYGCKQRKMSRLGLLRVGVWQNILRVWTQGYVGNKRGEGFILGGLYVIGPGCQVTLCKFCVELSCPALNSLSKMSICCKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBI4</accession>
    <name>A0AAR2LBI4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBI4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048409.1">
        <property type="protein sequence ID" value="ENSPNAP00000072012.1"/>
        <property type="gene ID" value="ENSPNAG00000036796.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286909"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF4">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="121"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="661" mass="73704" checksum="53853CE970CA4958" modified="2024-11-27" version="1">MALILTLTLTSALILTLTSTQNLILTLALSLALTSALRLRFHPAGPPPVALHIHPDIAFNPRRKYIHRGSRLNFTTSDSDSIQSFWSRTRRPPRNTDRGVNRDVLAHLARSANSSIKHRSSNVNFGLLNVRSLTGKGHLIQDVLRDCKLDFLCLTETWQHPNDFVSLNESTPSEFVYIYQPRNSGRGGGLAIIHRKHWKVLPVSAPLFHSFEYTAVHLPGSAPTILCTVYRPPKPNKDFLYDFAALLTHLSVLSPNMIIVGDFNIHMDNGNNLLSRDFTSCLDSFGLQQYINFPTHSKGHILDLVCCSGVTPTHCKADLFSLSDHKLISFNVNIIASKICVPRVITFRKINDIDPVIFSSAISDLPFVHSLSTLDELISHYNVNLQNLLNAFAPLKHRSVSFARSAPWFTSNLRLLKAKGRQLERLFRKTGLTIHREMYDNHILYYKDCIVSAKSAYYSGLVSSNEGSSKSLFSLFASLTKPPDPFPSHMHSPDFCNSLLSFFSDKISNIHQQLSSVRALYTEADSLFSSLSVCHSFSTFTLPSLSDVINIIHKSNSSTCQLDPLPTKLVKACLPTLAPLITTIICSSLNNGTVPLSLKTASVTPILKKPGLDPNNFDNLRPISNLPFLAKILEKVVASQLHSYLSTHNLYEQFQSGFRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LCQ2</accession>
    <name>A0AAR2LCQ2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LCQ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054530.1">
        <property type="protein sequence ID" value="ENSPNAP00000074383.1"/>
        <property type="gene ID" value="ENSPNAG00000036894.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="75" mass="7930" checksum="AAC4CB56B8BCE9D1" modified="2024-11-27" version="1">MVCVSAEHEPPKTSLCPQPSLSRSSWRVPEVDGLLIKLLHDFFDAGQVGTIVGCRSGNRPCDRKVAGLTPSAGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LE04</accession>
    <name>A0AAR2LE04_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Synaptotagmin-like mitochondrial and lipid-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">C2CD2L</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017557238.1">
        <property type="nucleotide sequence ID" value="XM_017701749.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LE04"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085216.1">
        <property type="protein sequence ID" value="ENSPNAP00000074760.1"/>
        <property type="gene ID" value="ENSPNAG00000002264.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429758"/>
    <dbReference type="CTD" id="9854"/>
    <dbReference type="GeneTree" id="ENSGT00530000063764"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098592">
        <property type="term" value="C:cytoplasmic side of apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035774">
        <property type="term" value="P:positive regulation of insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21683">
        <property type="entry name" value="SMP_C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039934">
        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040885">
        <property type="entry name" value="SMP_C2CD2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119:SF8">
        <property type="entry name" value="PHOSPHOLIPID TRANSFER PROTEIN C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18696">
        <property type="entry name" value="SMP_C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Synaptotagmin-like mitochondrial and lipid-binding" evidence="2">
        <location>
            <begin position="105"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="728" mass="79534" checksum="0084637A7AEAB733" modified="2024-11-27" version="1">MMSFQDVSWLVLVALFCVSLLVVLVWLVQFSLALLRPRRGARPQRSEESSWQLPLSLTHQTLTGGVWGSLLRLRFGRDGSASDTEAGVKGLLSSLFAFKSFREHWQRAWVRALNEQACRQGSSIQITFDDSLQLPTSASIDHVTCTEQSAHSMVLQCNCSIDTVTFPVTVTQQSPAAVSMDMYQITMDPMKAQLQVCLEEVEEEGLLVSWTFSQQPLPSLTVTPRQRVQRESSEAEVDVDMIAKLVEDTLYATQPAMILNLRACVSSPAFPREKKEGSHNSPQQNNALRRLLVRHLKASSVKKGCQAHVGELCCVLSLDVPALEKRTRFLTPSTSPGATLDWGEDLMLDLQPETKELKVRLVERNSKGEQFLPGHACLSLDLPRRSPNGLHKLSITPGHGLAPTATVTLELLYMELNDVRGCQNATPLRSSLTPSKKVDVDRTIMPDGTIVTTVTTIQSRPKLDRKLGDSPSRSPSKVEVTEKRPTVLTESFQSRASISPTPSTESPLSNGLDPVAETAIRQLTESASKAAKKTPTKRSTLIISGVSKVPITEDDSALSVGYAAAMDAALQGDHSPSGAPNHHGASPNQDAEETTPSDVSERPSVDDVESETGSTGALETRSLKDHKDITTPGSYHHHCKEIKVVGFLRSGTKLLFRRRHRQKDPSFSQSHEDVCNLGNDSSTASCSSSSRKKSGSFSRRLIKRFSFRSSKSKGKSSATNGGANTADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LHX3</accession>
    <name>A0AAR2LHX3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hyaluronoglucosaminidase</fullName>
            <ecNumber evidence="5">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CEMIP</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEMIP family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059009.1">
        <property type="protein sequence ID" value="ENSPNAP00000076150.1"/>
        <property type="gene ID" value="ENSPNAG00000012321.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428522"/>
    <dbReference type="GeneTree" id="ENSGT00940000153636"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd13938">
        <property type="entry name" value="PANDER_like_TMEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052252">
        <property type="entry name" value="CEMIP/CEMIP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055401">
        <property type="entry name" value="CEMIP_beta-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055400">
        <property type="entry name" value="CEMIP_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019316">
        <property type="entry name" value="G8_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039477">
        <property type="entry name" value="ILEI/PANDER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039473">
        <property type="entry name" value="TMEM2_PANDER-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025155">
        <property type="entry name" value="WxxW_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15535:SF15">
        <property type="entry name" value="CELL MIGRATION-INDUCING AND HYALURONAN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15535">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24606">
        <property type="entry name" value="CEMIP_beta-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24605">
        <property type="entry name" value="CEMIP_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10162">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15711">
        <property type="entry name" value="ILEI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13330">
        <property type="entry name" value="Mucin2_WxxW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01225">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51484">
        <property type="entry name" value="G8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52031">
        <property type="entry name" value="GG_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15" id="KW-0430">Lectin</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043747923" description="hyaluronoglucosaminidase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="domain" description="G8" evidence="18">
        <location>
            <begin position="29"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="830"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1350" mass="151419" checksum="AFD1A39258AB567F" modified="2024-11-27" version="1">MRTCVALLGAVLVLPIIQAVCPDKVPGLQPWTPGFSEAHHVTIGYGRKVLLTSSATVHSIEILNGGKLVIADTNLPILLRAKHILIGNKGELHIGSADCPYKGNLTISLYGRSDDSDEEHSYFGRKYVGIGTGGILEIHGQKKLSWTFLNKTLHPGEGNENSYHFERSWGNRGIIVHIIDPKTGEVLHNDRFDTYRSKDESRRLAQYVEGVENGQILAMVVNDEGSNNLEDTARKALSKLGSRHFHRLGFRHPWSFITVKGDTASAVEDHAIYQGTKASSEARSVRAFHSGYGEHFTITTVSQWAQEAEWTEWFDRDDERGSGDWEKLSDLHKAFPERLCSNPLDIEAETLDGIPANMTGEVFSKFDKNYGFVCLNKDQPQGICHNYRVRFLCGKLVRPQASISVDTHSNCSILELADEAQGWWPGDRLVVASTDYSMLQAEEFTVLPCLTCTSNQVKVEGKPQFLHMGEMVDGVDMRAEVGLLSRNILIRGEMEAGCYGSEACKFFSFDTFGGHLKVEHGFRGVHIQGVEFVHMGQQSMGHYPVHFHMNGDVDQHGGYDPPTYVKDLSIHHTFSRCVTIHGSNGLLVKDVVGYDALGHCFFTEDGPEERNTFEHCLGLMVKAGTLLPSDRDSKMCRDITDGAYPGYVAKPRQDCSATSTFWIANPNNNLIDCAAAGSEETGFWFIFHHVPTGPSEGMYSPGKTEHTPMGRFINNRAHSNYRAGMILDNGVKTTQANAKDKRPFLSLIGARYGPHQDADPFKPRVPALIHHFVAYKNQDHGAWLRGGDVWLDDCQFADNGIGLTLASGGTFPDDDGSRQEVKNSLFVGESENRGMPMSDSRTWGPGGLDHSGRTLPRGVDFPIRGMQIYDGPINIQNCTFRKYVALGGRHTSAFGFRLNNSWQSCPNNNVTDITFDSVPITSRVFFGEPGPWFNEMQMDGDKTTIFHDVDGSVSEYPGAFLVKEDNWLLRHPDCIDVPDWRAAICSGRFAQIYIQARNPANLNMKMVKDEYSEHPLELVGALGKRNHYQQYQPVITLAKSYTVHWDRAAPAELTVWLINFNRNDWITVGFCYPKGTNFTIISDLHNRLTKETRKTGVFMRTTQREKMSHTHQTRGYYYWDEDTGLLFLRVQAHNDKDDFAFCSVKGCERIKITAKIPKGSGPSDCMEMAYPKYAEMPVVDVPMPKKLPRSRLPQASEHFLEIKLESYNTRFFHIKEDFAYTEVNGKKIYQSEDGVHVTVLDGHTGKVLDTKGFRNAVLMGIPAQIENYINSLKDESIVMITSKGKLVTRGPWTKVLEKLGAEQNVKLKDKLAFVGFKGSFRPDWVRLAVDEERANLHQVLPMPIVRVMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LKH0</accession>
    <name>A0AAR2LKH0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation and early development. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPEB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LKH0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078362.1">
        <property type="protein sequence ID" value="ENSPNAP00000075259.1"/>
        <property type="gene ID" value="ENSPNAG00000016750.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155524"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008135">
        <property type="term" value="F:translation factor activity, RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000766">
        <property type="term" value="P:negative regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12723">
        <property type="entry name" value="RRM1_CPEB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12725">
        <property type="entry name" value="RRM2_CPEB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000054">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000086">
        <property type="entry name" value="Putative Cytoplasmic polyadenylation element-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.40:FF:000002">
        <property type="entry name" value="Putative Cytoplasmic polyadenylation element-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032292">
        <property type="entry name" value="CEBP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032296">
        <property type="entry name" value="CEBP_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034819">
        <property type="entry name" value="CPEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034977">
        <property type="entry name" value="CPEB1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT BINDING PROTEIN CPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566:SF9">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16368">
        <property type="entry name" value="CEBP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16366">
        <property type="entry name" value="CEBP_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16367">
        <property type="entry name" value="RRM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="326"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="581" mass="64575" checksum="52C80875E6233CD0" modified="2024-11-27" version="1">LETNAHMYNIYIYTKALCRKSSGFFSSNSLNRWDSDTPALSTCSNADIFRRMNTMLGNSLDLTGVCTTPSGKTKRDSLMDRPDSDLSAVRSRMLFPSNSQDSARGLPDVNELGLGLQSLSLSSWERPWSSQDSEQAAPNSSVSMLGMLGSPLGHLLNKPQGFSSNESNAGPSDFLEKFPALARLNSRSLLGSHSSSPVDSETSGFSSGSDHLSDLLSSLRISPSVPFLMSNVQKDPLKLSLGSRMDHDSSPLTPPPSSTPDGSSLSRRWPGASVWPNWDLLETPDGPFSIEREARLHRQAASINEATFTWSGQLPSRNYKNPVYSCKVFLGGVPWDITEAGLINTFNCYGPLSVEWPGKDGKHPRCPPKGNMPKGYVYLVFESEKSVRGLLQACTQDLLHPEGYSEYYFKMSSRRMRCKDVQVIPWVISDSNYVRCPSQRLDPSNTVFVGALHGMLNAEALASIMNDLFGGVMYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTPKFTKKVQIDPYLEDSMCQVCSRQPGPFFCRDQACFKYYCRSCWHWQHSMDILSNHRPLMRNQKNRDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLA2</accession>
    <name>A0AAR2LLA2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="27">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">ATP11C</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21 27">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 26">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Membrane</location>
            <topology evidence="27">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 27">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="27"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLA2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053712.1">
        <property type="protein sequence ID" value="ENSPNAP00000075534.1"/>
        <property type="gene ID" value="ENSPNAG00000019325.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158878"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000072">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000012">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF38">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="19" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="16 26 27" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 25 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17 27" id="KW-1278">Translocase</keyword>
    <keyword evidence="10 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="918"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="968"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="1037"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="1068"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="1109"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="28">
        <location>
            <begin position="38"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="29">
        <location>
            <begin position="854"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="29">
        <location>
            <begin position="1030"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="24">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>ATP</name>
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    </feature>
    <feature type="binding site" evidence="26">
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        <ligand>
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    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="508"/>
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        <ligand>
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    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="550"/>
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        <ligand>
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    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="573"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="604"/>
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        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="684"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="685"/>
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    </feature>
    <feature type="binding site" evidence="25">
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
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            <position position="801"/>
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    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="807"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="828"/>
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        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="831"/>
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        <ligand>
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    </feature>
    <feature type="binding site" evidence="25">
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            <position position="832"/>
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    </feature>
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        <ligand>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    <evidence type="ECO:0000256" key="9">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
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    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
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    <evidence type="ECO:0000313" key="31">
        <source>
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        </source>
    </evidence>
    <sequence length="1175" mass="133917" checksum="26B0295DC41CE9AC" modified="2024-11-27" version="1">MLRRRLNLLFGGDEKRVDSRTIYVGHRPGPATEAFIPPKFCDNRIVSSKYTVWNFLPKNLFEQFRRIANFYFLIIFLVQVIVDTPTSPVTSGLPLFFVITVTAIKQGYEDWLRHKADNEVNKYPVIVLEDGRKESEKIKVGDIVEVMEDETFPCDLILLQSSRDDDTCFVTTASLDGESNHKTHYTVPDTEKDLQSLSATIECEQPQPDLYKFVGRMYIYKTGQDPAVRSLGPENLLLKGATLKNTKKIYGVAVYTGMETKMALNYQGKSQKRSAVEKSINAFLLVYLCILISKAVVCTTLKYVWQSQFGQDEPWYNQKTQKEKDTYVYLKMFTDFLSFMVLFNFIIPVSMYVTVEMQKFLGSFFITWDKDFFDPEIQEGALVNTSDLNEELGQVEYVFTDKTGTLTQNNMEFIECCIDGFQYKNTDSMSELDGFCVTDGPVSKLQQKAGLEKEELFLRALCLCHTVQVKEAGPDEIDEIDGVQVDGLAAGLSQPDEERGFIASSPDEVALVKGAMKHGFTFLGLENKTMKIRNWQNDTEIYELLHVLNFDPVRRRMSVIVRNKSGETLLFCKGADSSIFPRVRPEEVDRIRMHVERNATDGYRTLCVAYKQLSPEEYAVADAGLRDARLALQDREEKLMVVYNQVETEMSLIGATAVEDRLQEEAAETMEALQGAGMKVWVLTGDKMETAKSTCYACRLFQRGTELLELTVRTLEDGGRKREERLHELLLDYHKKAVQDAPPVKAGVTRSWSNSNQEYGFIIDGATLSLVMNPSPDANSSRYKNLFLQICQNCTAVLCCRMAPLQKAQIVKLVKNSKGSPITLSIGDGANDVSMILEAHVGIGIKGKEGRQAVRNSDYAIPKLKHLKKLLLAHGHLYYVRIAHLVQYFFYKNLCFILPQFLYQFFCGYSQQPLYDAAYLTMYNICFTSMPILAYSLLEQHIAIEILLDNATLYREIAKNAMLRWGPFLYWTVLGIYQGLLFFFGVRYLFSNPALQDNGQVSGALPCFLRTPMLSEVMEWPFHTFCSVLSLQVFGNWSYGTIVFTVLVFTVTLKLALDTRHWTWINHFVIWGSLAFYVFFSFFWGGIIWPFLRQQRLYFVFANMLSSVSAWLVIILLILLSLLPDILLVVLRKPRGPHARKKKKCQSSAGSSQSSRVSIRPLLMRTFSDESNTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LN10</accession>
    <name>A0AAR2LN10_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BH3-interacting domain death agonist</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017547623.1">
        <property type="nucleotide sequence ID" value="XM_017692134.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037395851.1">
        <property type="nucleotide sequence ID" value="XM_037539954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LN10"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060946.1">
        <property type="protein sequence ID" value="ENSPNAP00000077945.1"/>
        <property type="gene ID" value="ENSPNAG00000033168.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108424231"/>
    <dbReference type="CTD" id="559425"/>
    <dbReference type="GeneTree" id="ENSGT00990000204811"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008637">
        <property type="term" value="P:apoptotic mitochondrial changes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001244">
        <property type="term" value="P:positive regulation of intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090200">
        <property type="term" value="P:positive regulation of release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010479">
        <property type="entry name" value="BID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35447">
        <property type="entry name" value="BH3-INTERACTING DOMAIN DEATH AGONIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35447:SF1">
        <property type="entry name" value="BH3-INTERACTING DOMAIN DEATH AGONIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06393">
        <property type="entry name" value="BID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="200" mass="22133" checksum="D0ECD7DE8266CCA8" modified="2024-11-27" version="1">MDANDNLDCVSLVFLTFLDQTPCQNKDLEKELHRLGYDLKLTTDSGGTKYAHHGLVDLSIECDGELETDGNIPTNPRDFLQGVLPEVHLALPVNPEEAQAVREVAAELIRIADELNLMIVSRAAEGLARKLNSTSSLESWHILLVQSVDNLLKEVPGVHTEQVVMALTFSLVKAVCEHTPPLLRGLYNVVMQYISPARPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LQ35</accession>
    <name>A0AAR2LQ35_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Target of myb1 like 2 membrane trafficking protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TOM1L2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TOM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LQ35"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050453.1">
        <property type="protein sequence ID" value="ENSPNAP00000078693.1"/>
        <property type="gene ID" value="ENSPNAG00000032689.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156940"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14238">
        <property type="entry name" value="GAT_TM1L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.160:FF:000001">
        <property type="entry name" value="TOM1-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000003">
        <property type="entry name" value="TOM1-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014645">
        <property type="entry name" value="TOM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856:SF31">
        <property type="entry name" value="TOM1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856">
        <property type="entry name" value="VHS DOMAIN CONTAINING PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036948">
        <property type="entry name" value="TOM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="5">
        <location>
            <begin position="36"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="6">
        <location>
            <begin position="237"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="181"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="426"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="59923" checksum="3EB725B9377E7CBA" modified="2024-11-27" version="1">MFTASFTASLCWVVDKMEFLLGNPYSTPVGQCIEKATDGSLQSEDWTLNMEICDIINETEDGPKDAIRAVKKRLNGNKNYREVMLTLTVLETCVKNCGYRFHALVTTRDFIDGVLVKVISPKNNPPAIVQDKVLALIQAWADAFRSSPDLTGVVHVYEEMKRKGIEFPRSDMETLSPIHTPQRLQNAPEGDHKYNAPVQPKPQPQPGSVPAYSAPLPHTSPQIPNLQMSGPINPTPEQICRLRSELDIVRGNTKVMSEMLTEMVPGQEDPSDHELLQELNRTCRAMQQRIVELISRVSNEEVTEELLHVNDDLNNIFLRYERYERFRSGRTAHGVNNGVLSEATEDNLIDLGPGSPAVVSPRISATPPTNLPSTVTPARSASPATLSSRLAGLDVGADSVSGTLSSLSSCQPQDDFDMFAQTRTGTLSDQQKNTPFEDTQAASGAAPTLDVRQQNSAGDALVQSSVMDDIEEWLCTDVKGDEGEEGVTSEEFDKFLEERAKAAEMVPALPTPPGGDPAAAVGSAASAVTDCPPVRPNTDGCPTLKSPCPPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LQD4</accession>
    <name>A0AAR2LQD4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LQD4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041519.1">
        <property type="protein sequence ID" value="ENSPNAP00000078785.1"/>
        <property type="gene ID" value="ENSPNAG00000035615.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282520"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="38"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="322" mass="36479" checksum="D093351E8088E1E6" modified="2024-11-27" version="1">FLGTNFSNITLFIIEGFKDRHLILFTVFLTMYVLILGGNSMIIYLVRTDSKLKSPMYFFLHNLSFSDMIYTSVTIPNMLSGFLVEVKTVSKVGCFVQMIFFLSMAVTGRALLTVMAFDRYLAICHPLRYTSIMTRRIRYLLIFLAWLFGWTLIVPAMSLALPLPFCGPNIVKHIFCDHTSVVRLACTDTTRNNIVSLTFALFAIISTFLLILASYICIGKTISKMGSAERLKAAATCVSHLIVVCISYVSASCVYISYRVATFDPDVRLIIAVLYSVLTPLLNPFIYSLRNKELQDALKRIPWKRKTVLSLTLHDRLSLVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZN2</accession>
    <name>A0AAR2LZN2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Cadherin EGF LAG seven-pass G-type receptor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="35 36">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor that may have an important role in cell/cell signaling during nervous system formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000043524.1">
        <property type="protein sequence ID" value="ENSPNAP00000082005.1"/>
        <property type="gene ID" value="ENSPNAG00000020670.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159839"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15991">
        <property type="entry name" value="7tmB2_CELSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000021">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000059">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000123">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000047">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000020">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000089">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000286">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000010">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000023">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000029">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000038">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000040">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000113">
        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000044">
        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056286">
        <property type="entry name" value="Cadherin_CELSR1-3_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF36">
        <property type="entry name" value="CADHERIN EGF LAG SEVEN-PASS G-TYPE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23592">
        <property type="entry name" value="Cadherin_CELSR2_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="15 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 23" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="20 24" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="14 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 27" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0807">Transducer</keyword>
    <keyword evidence="8 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043366849" description="Cadherin EGF LAG seven-pass G-type receptor 1" evidence="27">
        <location>
            <begin position="21"/>
            <end position="3020"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="2463"/>
            <end position="2486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="2498"/>
            <end position="2516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="2522"/>
            <end position="2544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="2565"/>
            <end position="2585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="2605"/>
            <end position="2626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="2647"/>
            <end position="2668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="2674"/>
            <end position="2697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="2786"/>
            <end position="2805"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="240"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="348"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="456"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="562"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="667"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="769"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="872"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="978"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="34">
        <location>
            <begin position="1102"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="1281"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="1341"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="1381"/>
            <end position="1419"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="28">
        <location>
            <begin position="1420"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="1658"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="28">
        <location>
            <begin position="1698"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="1880"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="1916"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="30">
        <location>
            <begin position="2011"/>
            <end position="2058"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="32">
        <location>
            <begin position="2043"/>
            <end position="2116"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="31">
        <location>
            <begin position="2272"/>
            <end position="2454"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="33">
        <location>
            <begin position="2461"/>
            <end position="2698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="2812"/>
            <end position="2985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="2839"/>
            <end position="2851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="2886"/>
            <end position="2900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2911"/>
            <end position="2924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="2944"/>
            <end position="2955"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="1329"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="1367"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="1684"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="1944"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="2011"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="2013"/>
            <end position="2030"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="2032"/>
            <end position="2041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00071749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="3020" mass="334483" checksum="9430F2860693C0D5" modified="2024-11-27" version="1">MDLPMKWIWFGALVQLPLYGCFDLHFPESLQPGAALVNVSLGAGWMYRIDRTLTAKWIGRAVRVSSNDGVVSLARKVHCSRFTRHPAQLYLRITSLLSGNVVLIHFNTFVHGQNCFIKYKRKTRQLDAYIELHSGYGACFNSGMLPISLYEHLPAFARQSQLLLSTCKEEKFGNKEASETEQCLPRHARFLCTVPTFQENVQLLQVQLNFRPNRGQSVAVSSEQWVKRLKRNVNSAPQFQLPNYQVSVPENEPSGTRVITLKAFDSDDGDAGVVEYDMEALFDSRSNDYFQINPETGGITTLQPLDRELKDTHVFKVTATDKGTPKRSATAYLTITVSDTNDHRPVFEQTEYRVSIRENVEVGFEVMTIRATDGDAPSNANMIYKIVNEVEVNSGFEIDPRNGLVKIKVRPDREVQSKYQLIVEANDQGKDPGPRSATATVHITVEDENDNYPQFSQKRYVVQVLENIAVNSEVAQVKATDKDAGNNAKVHYSIISGNVKGQFYIHSPTGVIDVINPLDYEMIREYNLRIKAQDGGRPPLINGTGMVVVQVVDVNDNAPMFVSTPFQASVLENVPVGHSVIHIQAIDADSGDNALLEYRLTDTSPSFPFVINNSTGWVTVSLELDRETTEFYTFGVEARDHGVPIMSSSASVSVTVLDVNDNIPTFTQKMYNLKINEDAAVGASVLTITAVDRDANSVVTYQISSGNTRNRFAITSQSGGGLITLALPLDYKQERQYVLTVMASDGTRFDTAQISINVTDTNTHRPVFQSANYQVLVSEDKPVGSTVVVISATDEDTGENARITYVMEDNVPQFKIHPDTGAITTQIEIDYEDQASYTLAIIARDNGIPQKSDTTYVEIIVLDANDNAPQFLRDIYQGTVFEDAPIYTSVLQVSASDRDSGSNGRVSYTFQGGDDGEGDFFIEPYSGIIRSARKLDRENVATYNLKAFAVDKGVPPLKAAVDIQVTVLDINDNAPVFEKDELYVYVEENSAVGSTLARISATDPDEGTNAQILYQIVEGNVPEVFQLDIFSGDLIALTDLDYEAKMEYLIVVQATSAPLVSRAIVHVRLVDVNDNYPVLQDFEIIFNNYVTNKSNSFPAGVIGKVPAHDPDVSDKLLYTFVEGNELSLLILNQDTGELKLSTDLDNNRPLEATMKVTVSDGLHQVSALCTLRVTIITDDMLTNSITVRLENMSQKRFLSPLLGLFAEGVAAVLSTSREGIFVFNVQNDTDVGAPILNVTFSALKPGGGPGRFFPTEELQEQLYLNRSLLRLISTQRVLPFDDNICLREPCENYMKCVSVLKFDSSAPFVASNTLLFRPIHPVNGLRCRCPDGFTGDYCETEIDLCYSAPCKNNGRCRSREGGYTCECPEDFTGEHCEVNVRSGRCVSGVCKNGGRCVDLLVGGFMCQCPEGEFEKPYCQMTTRSFPGHAFITFRGLRQRFHFTLSFMFATRERNALLLYNGRFNEKHDFIAVEIVEEQIQLTFSAGESKSTVSPFIATGVSDGQWHTVWLHYYNKSVLTVVKLFVFIFCVFCVSLYGVWLKIGLNYPNIGRLGLPHGPSEEKVAVVAVDDCDVAMAVRFGGQIGNYTCAAQGTQTGKKKSLDLTGPLLLGGVPDLPEDFPIRNRDFLGCMRNLIIDSKPIDMASFIANNGTAAGCPAKSNFCSRSACHNGGVCVNKWNTHACNCPLGYGGKNCEHVMPSPLHFDGHAMVSWSDPDVTIAIPWYISLMFRTRKSTGTLLHAVAGELSKFSLIVSVQLRFQVFLGSRRVALLEFLQVRVNDGEWHHILVELKSGKDGKDIKYMALVSLDYGMFQRTVEIGNELPGLKVRGLWIGGLLKKDGSVLNGFNGCMQGVRMGETSTNTVNINIRQALRLRVKDGCDIPDACMSSMCPTHSHCRDNWASHSCMCEPGYFGRDCVDACLLNPCEHTSSCVRKPATKHGYTCECGHNYYGQYCEHRGDQPCARGWWGYPTCGPCNCDVNKGFKRDCNKTTGVCSCKDNYYRPAGSDTCYPCECFHLGSQNRTCDPLTGQCPCKMGVVGRQCNRCDNPFAEVTANGCVVVYEGCPKAFDAGIWWPKTMFGGPAAMNCPKGSSGTAVRHCSDENGWLPPELFNCTSHSFAKLRKEVEDIHANSSRLDGERSKSLVAMLHTATGHTQHLYGSDVRTAYELMRSVLQRESLQQGFDLSATHDSTFNQDIVRAGSAILDPENKGHWEQIQHSDGGSASLLHHFEEYSSTLVQNMRKTYLKPFTIITNNIIVSVDFLDSSTPDQSALPRFQEIQEAYSKELESSVFFPDLYIKPADIPEGTHSPALTSPAPSAKKRRHAELADPPSVAMVIIYRSLGQLLPENYDPDRRSLRLPTRPVINSPVVSVVAHREGEFVSVPLQRPITLTFRLLETQERTKPVCVYWNHSILLSGSGAWSAKGCELIFRNSTHISCQCNHMSSFAVLMDISKREHGDVLPLKVVTYTTVSASLVALLITFLLLAILRKLRSNLHSIHKNLVAAIFLSELIFLTGINQTDNPFVCTVVAILLHYSYMCTFAWMFVEGLHIYRMLTEMRNINQGHMRFYYAIGWGIPAIITGLAVGLDPQGYGNPDFCWLSVHDTLIWSITGPITFVVLINIAIFVLATKASCGRRQRTFEKSGAISALRVAFLLLLLISATWLLGLMAVNSDVLTFHYLFAILSCIQGICIFFFHCILNKDVRKNLKSVFTGKKGSVEESSATRATLLTRSLNGNTYIEDGCLYRTPIGESTVSLESSVRSGKSHSNWITNCSKNYASKYLSDNVSVHMYVIMLVKIVAYFVNVYLHMLSDSDSDSELSVDEHSSSYASSRSSDSEEEERSSKPKWNNERSPIHSTPKVDAVSNHMKPYWPVEPPTASESEGTGGPEKLRVETKANVELHKGNKLSPNGELSQSEGPPSQPSSNQLPRRGILKNKITYPPPLTDKNMKNRLREKLSDYNAPIPTVPCKSPSEGSNEGLRSGTESNSIIIKPPALQLTTTLNGVTMELGTSPVLAAEETDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M4U6</accession>
    <name>A0AAR2M4U6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EPHX2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M4U6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069411.1">
        <property type="protein sequence ID" value="ENSPNAP00000081936.1"/>
        <property type="gene ID" value="ENSPNAG00000032099.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000077867.1">
        <property type="protein sequence ID" value="ENSPNAP00000039606.1"/>
        <property type="gene ID" value="ENSPNAG00000032099.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158614"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02603">
        <property type="entry name" value="HAD_sEH-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000067">
        <property type="entry name" value="Bifunctional epoxide hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="255"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="553" mass="61950" checksum="78F0A263779B462B" modified="2024-11-27" version="1">MYKAALFGLWGGVLTPHPLKSFQKVEKVLNLPRDFIWNTLVKGGDQNPLFRAERGKLTLTQMFPELEADCQKEADSQGLPLPAQFSAQTLFDEVRRVEFNKTVLDAAATLQRQGISTCVLANIWVDDTPQRDNTAKVLSVLESHFDLVVRSCHVGAKLPEPAVLHKALDKLNVKPHEALWLDISEESVKAAESLGMTAVHITDISEALKKVENLTGIKLTGEQVPRFCGPEEVSHGYVTVKPGVKIHYVEMGDGPPVILCHGFPESWFSWRYQIPALADAGFRVIAADMKGYGDSSAPAEIEEYSQESLSQDMVTFMDKLGLAQATLVGHDWGGTLVWNMAQCHPERLRAVASLNTPLFPVDPNKDPMDKIKSIPIFEYQLYFQEPGVAEAELEKNLARTFKLLFTTSHDNMVASTGVLKKGGLFVGLPEEVPRSSMLSEEALQYYVQQYTKSGFRGPLSWYRNVERNWRWLCSRPRGKILMPALMVTAGQDKVLLPSFSTGMEQMIPNLTRAHIEDCGHWTQMERPAELNKILIPWLKETHEKASIPIFPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M5I3</accession>
    <name>A0AAR2M5I3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017547507.1">
        <property type="nucleotide sequence ID" value="XM_017692018.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M5I3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082012.1">
        <property type="protein sequence ID" value="ENSPNAP00000082527.1"/>
        <property type="gene ID" value="ENSPNAG00000031981.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108424185"/>
    <dbReference type="CTD" id="572060"/>
    <dbReference type="GeneTree" id="ENSGT00940000157090"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04115">
        <property type="entry name" value="Rab33B_Rab33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000516">
        <property type="entry name" value="RAB33B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041822">
        <property type="entry name" value="Rab33A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="235" mass="26247" checksum="E6B4BC8C4D911A57" modified="2024-11-27" version="1">MAEESSLSSLEFSSSLPPPRTRIFKIIVIGDSGVGKTCLTYRFCAGRFPDKTEATIGVDFREKVIEIDGEKIKVQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDVTSAASFRSLPAWIEECRQHALGQEVPRILVGNKCDLRHLAQVSTELAQQFADAHSMPLFETSAKNPQGGKEGGRNSSDHVEAIFMTVAHKLKSQKPLVLSQPAGGNTVTLSRNEEEEQRGNWGCSCWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWW9</accession>
    <name>A0A3B4BWW9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017546206.1">
        <property type="nucleotide sequence ID" value="XM_017690717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWW9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004182"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007431.2">
        <property type="protein sequence ID" value="ENSPNAP00000004182.1"/>
        <property type="gene ID" value="ENSPNAG00000010624.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423425"/>
    <dbReference type="CTD" id="336944"/>
    <dbReference type="GeneTree" id="ENSGT01030000234667"/>
    <dbReference type="OMA" id="WCFSKKY"/>
    <dbReference type="OrthoDB" id="9828427at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000035">
        <property type="entry name" value="C-C chemokine receptor type 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF943">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="54"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="368" mass="41480" checksum="2A31B63FD6A23543" modified="2018-12-05" version="1">MLQDVDFSNETDDNPFYDLGVDETSPCDLKGNHGQQLTILTCVYSLICALGLVGNILVLVTYAFYRKAKTMTDIYLVNVALADLLFVISLPLIIYNEQYSWSMGSWACKLLTGTYSINLYCSMLLLACISGDRYVAIVKASRSIGIRTKAQIYSRLICSIIWLLAIALSLPTFIFYDRYEEKSFDGIVEVECVLSLETGTTANLMRVLVPSTQVTVGFFVPMFVMGFSYCSIVLTLLRVQNYQRHKAVRVVLAVVIVFILCHLPYNILILVHTGSLFGERSCEAEQNILLALSITRSVAYLHCCLNPILYAFIGVKFRNHFCQIMEDLWCLGKRYFSSARSSQQTSELYIAVHKSSEVNHENPSSFTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYT0</accession>
    <name>A0A3B4BYT0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoinositide 3-kinase regulatory subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PIK3R5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017540924.1">
        <property type="nucleotide sequence ID" value="XM_017685435.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017540926.1">
        <property type="nucleotide sequence ID" value="XM_017685437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYT0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004743"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006498.2">
        <property type="protein sequence ID" value="ENSPNAP00000004743.1"/>
        <property type="gene ID" value="ENSPNAG00000011138.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413086"/>
    <dbReference type="CTD" id="23533"/>
    <dbReference type="GeneTree" id="ENSGT00530000063753"/>
    <dbReference type="OMA" id="VLCQHSL"/>
    <dbReference type="OrthoDB" id="9932678at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005944">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IB"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019522">
        <property type="entry name" value="PIK3R5/6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15593">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15593:SF2">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10486">
        <property type="entry name" value="PI3K_1B_p101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="307"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="380"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="864" mass="95653" checksum="839F641AFEC10857" modified="2018-12-05" version="1">MQHTSCTEDRIQHALDRCLDGLQSSSTLTQSWNTGWCMNRWSLEELLRRDPENFLILLQQILTRARKVQEQSEYKLVAPLAIMFESTLLQTPYSPDGDLLAEACEVFHCFLTWPEPYSTVCHGLLTTLQQELRAPGISYHRLVRDEQGLSASTHGSRTITVLLLNPADVPAAFLSVLKQLGGAEVSHRDASITLIKHAFQSTLGAKYPLTAIHTALQSLSEEELAQTLSSVTELLESSAAMPDVSTARACAAQRLEEVREKLKIPACNSRNTDGMLQILRLPTAKCYTIDWDMDNFDVLNKFLESEPDLASQQTSEGAAEEDEDDEDADEEEDLEEMEDFTSLGYVDPRASMFSTVSSLSTASKDSMFSTYSLASSECSPLSTLSSSSQASGTDSDFCEDVEEESPVPPVLGKPKSSAGFSKRLSRLFKPRSSHSLCRAKSLGNAEAKDILPSVRSKRSNSVPHQALMCSSELGLTESLLAQQALGCVRYRRRPILSSDEAEEPRAALLRVLVFGADHVAGRVARAYSSLRRRERDYPRLSRAFRLRFFFIPVRRDPPGNGTVNQFSASPLKTIQDINADQPEDSTNDIAALLGMLDPWYERNTLCLLDLPLHVVCKQTSKPEPDLLQDSVEEGLPILADLLLYYCRNATRPALVQLYQAELTLAGGETRTEVFVHSLELGHTAGTRAIKAMGAASKRFGIDGDREALPLSLELVYNQVVISGRSQKTRVEKVCTSVNLTKACRNPEELDPKIECLQLTMTEVLKRQNSKSKKSFNQQLSTTRAKVDKVRVSGSGNTTFAVCLDQDEKKILQSVTRCEVSVCYKPDSCANWPRFRALSSPVQPLQPTFCSLLCLPIATFSGPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C072</accession>
    <name>A0A3B4C072_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 26C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">VPS26C</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. Component of the retriever complex, which is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1). The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes. In the endosomes, drives the retriever and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. Component of the heterotrimeric retriever complex consisting of VPS26C, VPS29 and VPS35L; within the complex interacts with VPS35L. Interacts with SNX17 (via C-terminus); the interaction is direct and associates SNX17 with the retriever complex. Interacts with SNX31; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS26 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539304.1">
        <property type="nucleotide sequence ID" value="XM_017683815.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C072"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007397.2">
        <property type="protein sequence ID" value="ENSPNAP00000004211.2"/>
        <property type="gene ID" value="ENSPNAG00000010670.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411987"/>
    <dbReference type="CTD" id="10311"/>
    <dbReference type="GeneTree" id="ENSGT00950000183064"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000008">
        <property type="entry name" value="Down syndrome critical region protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000009">
        <property type="entry name" value="Down syndrome critical region protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004211.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="297" mass="33142" checksum="7F34B89DAA896BF7" modified="2024-11-27" version="2">MSVALDLRLKRANKVYHEGEVLAGVVVITSKEAVQHQGISLTMEGLVNLQLSSKSVGVFEAFYNSVKPIQLISSNIEVAKPGKVPGGKTEIPFEFPLQAKGNKMLYETYHGVFVNIQYTLRCDMRRPLLAKDLSKTCEFMLHCQPQKSKVQPTPVDFTITPETLQNVKERTSLPKFVIRGHLDATNCVITQPLTGELVVESSEVAIKSIELQLVRVETCGCAEGYARDATEIQNIQIAEGDVCHGLPIPIYMVFPRLFTCPTLETTNFKVEFEVNVVIVLHDDHLITENFPLKLCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0K7</accession>
    <name>A0A3B4C0K7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="2">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CD38</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0K7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004351"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007160.2">
        <property type="protein sequence ID" value="ENSPNAP00000004351.1"/>
        <property type="gene ID" value="ENSPNAG00000001438.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017291"/>
    <dbReference type="OMA" id="PACNRMM"/>
    <dbReference type="OrthoDB" id="10028716at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF9">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="284" mass="31311" checksum="D826DFE023A02E9E" modified="2018-12-05" version="1">MHREASRRKRCLLLGVVVVVVVVVVVVVVVVVLAIVLGLTLTRYSDHLRSTVIGRCESFLKKHTDVSRVNDCERIWGAFEEAYVGKDPCEVPAEAYDPLISSVTQHVICGTMLFWSKTKALAHAFTDNRECLVTLEDTLLGFVFDELTWCSKNGSKETFTTDCPGWSECQNNPVRSFWIRASANFAATSCGNVSAMLNGSLESPFSSTSVFGSVEVKSLDPNKVDDLTILLVTKETDTTTCASSSFQSLRSALDPKIAYNCREVPYNRVENCISDPEIPCSDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C1W1</accession>
    <name>A0A3B4C1W1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GGA1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GGA protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C1W1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004669"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006657.2">
        <property type="protein sequence ID" value="ENSPNAP00000004669.2"/>
        <property type="gene ID" value="ENSPNAG00000007197.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159613"/>
    <dbReference type="OMA" id="GQIADNW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027422">
        <property type="entry name" value="GGA1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041198">
        <property type="entry name" value="GGA_N-GAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905:SF6">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905">
        <property type="entry name" value="GOLGI-LOCALIZED, GAMMA-ADAPTIN EAR CONTAINING, ARF BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18308">
        <property type="entry name" value="GGA_N-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="VHS" evidence="11">
        <location>
            <begin position="43"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="13">
        <location>
            <begin position="171"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="12">
        <location>
            <begin position="381"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004669.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="55780" checksum="689DD03C5419DEE4" modified="2024-11-27" version="2">MVCGIGMVFGVGLVLVWCSVCGVCMFVWYWYDVWCLHVIPLFSPQVALRLLAHKIQSPQEREALQTLTLLEACMNNCGKRFRAEATKFRFLNELIKVLSPKFLGSWSSAQVKEKITEVLYSWTQWLKDEPKVQEAYRMLKKQGLVKSDPKLPDSVLMPPPSPRQEVSVFDDEDKAKLLSRLLKSGRPEDLETANRLIKSTVREEQEKMEKEGRRISTLEEVEKCTSELRLLLNQHTHHTAEMKDLYERCDRLRPNLFRLASDTLEDDETLAGILKWNDELSHVMNQYRERTQQEEREMGAEPMHNNDNSQGLLFYCAHAQAEIPLLGNTSCRLRAVISLFREEHMTRSEFIQPIITPGFPKPLPPTSLQDIHVPLGSIKPSHLKPITVFDRHSVRVSLHFTKETPPSRPDVAAFVISAVNTSALPVSDFLFLAAVPKTMQLKLQPATGTALPPYSPLLPPAAISQILLLSNPLRVRLKFTLCGFSDAVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2V8</accession>
    <name>A0A3B4C2V8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2V8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003896.2">
        <property type="protein sequence ID" value="ENSPNAP00000006242.1"/>
        <property type="gene ID" value="ENSPNAG00000012295.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000241327"/>
    <dbReference type="OMA" id="PQCNKPQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="134" mass="15032" checksum="C799719ABDD34263" modified="2018-12-05" version="1">MNLSIVNLSVTNLSIINLSVTNLSVINFLKPQCNNPQCNKPQCNKPQVINLTVINLIKTQCNKLQCNKPQCNKPQCNKPQCNKPQSNKLQCNTPLCNKPQCNKPQNNKPQCNKPQCNKPQSNKPQCDKPHQTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3J5</accession>
    <name>A0A3B4C3J5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ACOX3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Oxidizes the CoA-esters of 2-methyl-branched fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2S)-pristanoyl-CoA + O2 = (2E)-pristenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40459"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:77099"/>
            <dbReference type="ChEBI" id="CHEBI:77293"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:38960"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexadecanedioyl-CoA + O2 = (2E)-hexadecenedioyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40275"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:77075"/>
            <dbReference type="ChEBI" id="CHEBI:77085"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:40276"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hexadecanoyl-CoA + O2 = (2E)-hexadecenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40167"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40168"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tetracosanoyl-CoA + O2 = (2E)-tetracosenoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:40319"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:74693"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40320"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555097.1">
        <property type="nucleotide sequence ID" value="XM_017699608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3J5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005600"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005017.2">
        <property type="protein sequence ID" value="ENSPNAP00000005600.1"/>
        <property type="gene ID" value="ENSPNAG00000001940.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428549"/>
    <dbReference type="CTD" id="8310"/>
    <dbReference type="GeneTree" id="ENSGT00940000159423"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016402">
        <property type="term" value="F:pristanoyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01150">
        <property type="entry name" value="AXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000007">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000010">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034171">
        <property type="entry name" value="ACO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF390">
        <property type="entry name" value="PEROXISOMAL ACYL-COENZYME A OXIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17 19" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="22">
        <location>
            <begin position="164"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="23">
        <location>
            <begin position="311"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="21">
        <location>
            <begin position="521"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="709" mass="79824" checksum="AACF351168D2D5D6" modified="2018-12-05" version="1">MTLPEISGAKHGLDEELDTQDIPDLPSGPLDEYRKQASFNWKEMMRFMEGEEIIAFKQLIFRTLENDPLFARQPGEDVLVEKKRELTFRRCKQLFKYNFLTRAEMMQSPWKVTVLNDCLGMYDWSVGAKFFLNKAMFGGTVANSGSQRHLKFVQDIEDMKTFGCFALTELSHGSNTRAMRTTAKYDPKTQEFVINSPDFEAAKFWVGNLGKTATHAVVYAQLYTPDGVCHGLHSFVVQVRDPKTLLAMPGVMVGDMGKKLGQNGLDNGFAVFHNVRIPRENLLNKTGDVTPDGLYVTPFKDPNKRFGASLGALSGGRVSITRMAVVNLKLALIVAVRFSATRRQFGPKEDVEIPILDYQLQQWRLIPYLAAIYALDFFSKSFYKNFVEFQIGLFMKDNSDRQAELGREIHAISCSSKPLGSWTAQRGIQECREACGGHGFLSMNRLGDIRDDSDPNCTYEGDNNVLLQQTSNYLLSLLQHKQQAKGRIESPLETVNFLDDWKRILESRFTAGSVEECMNSAVPLAAYKWLVCFLLGESQRKLTQQKALGLGDFEARSNSQVYYCRSLAIAYIEHTVLQRFHDLTEDVETPAGLRSVLRRLCALYGLWTLTNHMAILYQGGYLSGKEPADFIQNAVLALCGQLKDDAVALVDVIAPPDFILNSPIGKADGELYKNLWATVLQGKNVLERPSWWAEFCSDKPKVGSLRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3P7</accession>
    <name>A0A3B4C3P7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CHHC U11-48K-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GTSF1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3P7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005461"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005247.2">
        <property type="protein sequence ID" value="ENSPNAP00000005461.1"/>
        <property type="gene ID" value="ENSPNAG00000005737.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156784"/>
    <dbReference type="OMA" id="LMPRHEL"/>
    <dbReference type="OrthoDB" id="10069248at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051591">
        <property type="entry name" value="UPF0224_FAM112_RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402:SF5">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="5">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CHHC U11-48K-type" evidence="5">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="181" mass="20993" checksum="FB676EAC67E753D9" modified="2018-12-05" version="1">MASRRKPSKASASQPKAPLRRWEEAHDEDYCNPEKLLVCPYDPNHQIRACRFPYHLIKCRKNHPELASQLWTCPFNARHLMPRHELSHHMSNCVDRCSVNTEYVESAESQHIFQVPVNTWTIPVCDEDWDQEENETATPAAPFVWGLSTSQLGQDRTDQAVTNRLTSSLRAPRVLPWNFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3P9</accession>
    <name>A0A3B4C3P9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CEP170 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3P9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005219"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005660.2">
        <property type="protein sequence ID" value="ENSPNAP00000005219.1"/>
        <property type="gene ID" value="ENSPNAG00000011502.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155103"/>
    <dbReference type="OMA" id="TYKTTLY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029300">
        <property type="entry name" value="CEP170_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15308">
        <property type="entry name" value="CEP170_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CEP170 C-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="153" mass="16771" checksum="11C06AD003C47072" modified="2018-12-05" version="1">MGGLVLASVSQLSAKIRQGVDKTASKIRILFKDKDKKWAELENIKKYLKSILPILTYKTTLYGVFLSYLVNSVVFYLQLSVIDIMLDPDGTLDALTNLGITSPLVTEIRVSPGAQNQSGSHRDGEGSASTPMFFSAEQDLGLMTKVQSNTIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5Q0</accession>
    <name>A0A3B4C5Q0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Photoreceptor implicated in non-image-forming responses to light.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5Q0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007197"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002243.2">
        <property type="protein sequence ID" value="ENSPNAP00000007197.2"/>
        <property type="gene ID" value="ENSPNAG00000005784.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286935"/>
    <dbReference type="OMA" id="KILFIWM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000044">
        <property type="entry name" value="Opsin, ultraviolet-sensitive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 14" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="40"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007197.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="310" mass="35034" checksum="BB5036244A2DA287" modified="2024-11-27" version="2">MLEMMELQKHLLFRKVDVPDHVHYTLAFIILMVGTLGVAGNTLVIFAFCSNRKLRTLPNFFIINLAVSDLLMALTQSPLFFINCLHTQWIMGELVCKLYAFCGALFGITSMITLLAISVDRYLVITHPLKAMQWNAKIRTFITIVLIWIYSLAWSLAPLIGWSSYVPEGLMTSCTWDYVSPSAGSRSYTLALCCCVFFVPLGIIISCYICMFLSVRRAGRDVSRLGSQMRKQDVSRPQHSVRSEWRLAKIAAVVIAVYVLSWAPYAWIKARDSGYADLLNPYSKTVPAVIAKASAVYNPFIYAIIHTKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6F0</accession>
    <name>A0A3B4C6F0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2'-deoxynucleoside 5'-phosphate N-hydrolase 1</fullName>
            <ecNumber evidence="7">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">DNPH1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-hydroxymethyl-dUMP + H2O = 5-hydroxymethyluracil + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:77099"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16964"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:90409"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:77100"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a purine 2'-deoxyribonucleoside 5'-phosphate + H2O = a purine nucleobase + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:51132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:142198"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a pyrimidine 2'-deoxyribonucleoside 5'-phosphate + H2O = a pyrimidine nucleobase + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:57852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:26432"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:142209"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 7">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017545907.1">
        <property type="nucleotide sequence ID" value="XM_017690418.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C6F0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006685"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003131.2">
        <property type="protein sequence ID" value="ENSPNAP00000006685.1"/>
        <property type="gene ID" value="ENSPNAG00000012654.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108420845"/>
    <dbReference type="CTD" id="10591"/>
    <dbReference type="GeneTree" id="ENSGT00390000001216"/>
    <dbReference type="OMA" id="EVLSWHV"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070694">
        <property type="term" value="F:5-hydroxymethyl-dUMP N-hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006195">
        <property type="term" value="P:purine nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000019">
        <property type="entry name" value="2'-deoxynucleoside 5'-phosphate N-hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03036">
        <property type="entry name" value="Nuc_phosphate_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051239">
        <property type="entry name" value="2'-dNMP_N-hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028607">
        <property type="entry name" value="DNPH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007710">
        <property type="entry name" value="Nucleoside_deoxyribTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15364">
        <property type="entry name" value="2'-DEOXYNUCLEOSIDE 5'-PHOSPHATE N-HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15364:SF0">
        <property type="entry name" value="2'-DEOXYNUCLEOSIDE 5'-PHOSPHATE N-HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05014">
        <property type="entry name" value="Nuc_deoxyrib_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="4"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="148" mass="16583" checksum="7E95FEDFFA703682" modified="2018-12-05" version="1">MHIYFCGSIRGGRQDVEVYQRIVKKLQTYGKVLTEHVSHAEVSEKGEDAVLDGDKAIHDRDVEWLASSDVIVAEVTQPSLGVGYELGRAVAMNKRILCLFRPSSGRVLSAMIRGADCRTESSVFKVLDYTEEEVEVILEKYFGELTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9E8</accession>
    <name>A0A3B4C9E8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Actin-binding Rho-activating protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Striated muscle activator of Rho-dependent signaling</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as an activator of serum response factor (SRF)-dependent transcription possibly by inducing nuclear translocation of MKL1 or MKL2 and through a mechanism requiring Rho-actin signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Binds F-actin and ABLIM1, ABLIM2 and ABLIM3. Interaction with ABLIM2 and ABLIM3 enhances activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017573763.2">
        <property type="nucleotide sequence ID" value="XM_017718274.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9E8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007034"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002503.2">
        <property type="protein sequence ID" value="ENSPNAP00000007034.2"/>
        <property type="gene ID" value="ENSPNAG00000002481.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439721"/>
    <dbReference type="CTD" id="324520"/>
    <dbReference type="GeneTree" id="ENSGT00940000166055"/>
    <dbReference type="OMA" id="PKRSECS"/>
    <dbReference type="OrthoDB" id="9871914at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1540:FF:000001">
        <property type="entry name" value="Actin-binding Rho-activating protein a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1540">
        <property type="entry name" value="Costar domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026111">
        <property type="entry name" value="Abra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027817">
        <property type="entry name" value="Costars_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038095">
        <property type="entry name" value="Costars_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22739:SF21">
        <property type="entry name" value="ACTIN-BINDING RHO-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22739">
        <property type="entry name" value="STRIATED MUSCLE ACTIVATOR OF RHO-DEPENDENT SIGNALING-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14705">
        <property type="entry name" value="Costars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01283">
        <property type="entry name" value="Costars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Costars" evidence="18">
        <location>
            <begin position="267"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="118"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007034.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="344" mass="39202" checksum="9CD78B828502EC74" modified="2024-11-27" version="2">MSTRTAQESRPFSRAVRKIKVAAAVTSLAKSWQNWANEHSCKQDTIPSGWMPDSIIQDEKEKERNEVKLQVMPRVVVVNDEDKADSKIKTGTVSKAIEPKRSEYGHDLVSSIKEKIQTNQMTAEEPRAFLGNESPTRRRLCSGKASASVKGLGQREESKMGSRSSSLDTEDSGLGEDAALSDNSDQSEVERKKPISRPKIRVTTMGDIRSKWQKFAAEHEEGQKLNPFSDDFDYEHAMAIRLHKGDAGYGRPKEGSKTAQRADRAQKHIRREMEEMCFIIRDMGQRDRDGRIYITFGRLFDRYVKISDKVVGILLRCRKHKMVEFEGEMLWQGQDDHVKIILLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CA12</accession>
    <name>A0A3B4CA12_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCN family member 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cellular communication network factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Connective tissue growth factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Is involved in the stimulation of osteoblast differentiation and has a critical role in osteogenesis. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer. Interacts with TSKU.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553223.1">
        <property type="nucleotide sequence ID" value="XM_017697734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CA12"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002118.2">
        <property type="protein sequence ID" value="ENSPNAP00000007254.1"/>
        <property type="gene ID" value="ENSPNAG00000013134.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427520"/>
    <dbReference type="CTD" id="321449"/>
    <dbReference type="GeneTree" id="ENSGT00940000155019"/>
    <dbReference type="OMA" id="ERDPCDH"/>
    <dbReference type="OrthoDB" id="365605at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007492">
        <property type="term" value="P:endoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030903">
        <property type="term" value="P:notochord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031099">
        <property type="term" value="P:regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000036">
        <property type="entry name" value="Connective tissue growth factor (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000015">
        <property type="entry name" value="CYR61 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012395">
        <property type="entry name" value="IGFBP_CNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF7">
        <property type="entry name" value="CCN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036495">
        <property type="entry name" value="IGFBP_rP_CNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017298640" description="CCN family member 2" evidence="16">
        <location>
            <begin position="24"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="19">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="18">
        <location>
            <begin position="97"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="17">
        <location>
            <begin position="252"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="345" mass="38263" checksum="04D4584392AD57E0" modified="2018-12-05" version="1">MFSGMSLRLVSVLCFTFFSWVVAQECTGQCHCPDVPPQCPPGVSLVPDACGCCRVCAKQLGELCTERDVCDPHKGLYCDYGSPSNRRIGVCTARDGATCVFGGMVYRSGESFQSSCKYQCTCLDGAVGCVPLCGMDVRLPSPDCPSPRRVKVPGKCCEEWVCDSPHTTMFVGSALAAYREEETYGPDLSMMRENCLVQTTEWSACSKTCGLGISTRVTNDNRECRLEKQSRLCMVRPCESHLEEKIRKGKKCIRTPRVSKPMKFEISGCTTTKAYRPKFCGVCTDGRCCTPHRTATLPVEFKCPDGQVMKKQMMFIKTCACHYNCPGENDIFESMYYKKMLGDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAK9</accession>
    <name>A0A3B4CAK9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555814.1">
        <property type="nucleotide sequence ID" value="XM_017700325.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037389255.1">
        <property type="nucleotide sequence ID" value="XM_037533358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAK9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008295"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000380.2">
        <property type="protein sequence ID" value="ENSPNAP00000008295.1"/>
        <property type="gene ID" value="ENSPNAG00000013913.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428936"/>
    <dbReference type="CTD" id="114457"/>
    <dbReference type="GeneTree" id="ENSGT01030000234579"/>
    <dbReference type="OMA" id="DHIPTYQ"/>
    <dbReference type="OrthoDB" id="5912413at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042472">
        <property type="term" value="P:inner ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048840">
        <property type="term" value="P:otolith development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048923">
        <property type="term" value="P:posterior lateral line neuromast hair cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000194">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1660:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01107">
        <property type="entry name" value="Na_K_ATPase_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF26">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00391">
        <property type="entry name" value="ATPASE_NA_K_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="293" mass="34261" checksum="3B54FD7C361AE4BD" modified="2018-12-05" version="1">MAKDDKKNEWKEFFWNPRTREFCGRTASSWGLIFLFYLAFYIFLAGLFTLTMYVMLLTLDDNTPTYQDRLATPGMMIRPRSEDLEITYTKENTESWDMYVQALNRFLSPYNDSVQEQKNHECTPDKYFIQDDSGNVKNNPKRSCQFKRSILEDCSGLYDRYYGYNDGKPCIIIKLNRVIGMLPGKDGQTPSVSCGPRRYKIGKDEWADDTDKMGEVVYFPRNGTFNLMYYPYYGKKAQVNYSQPLVAIKFLNMTLNTDINVECKINANNIPTNNERDKFAGRVSFKLRINSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CE25</accession>
    <name>A0A3B4CE25_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RAD17</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CE25"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014860.2">
        <property type="protein sequence ID" value="ENSPNAP00000008724.2"/>
        <property type="gene ID" value="ENSPNAG00000003080.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286927"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="89"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008724.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="630" mass="71313" checksum="29997615AD4F4B18" modified="2024-11-27" version="2">VIEKPTINVISAYKQKLETKFHSINEGISQHGKSTHLNEIYTELYITEGGSGEVNDEHEVRQIEAASRKPATEEKPIKCNDLFKDRPIRTVLTKGVAGVGKTFSVQKFILDWAEGKANQDIHFICTLPFRELNLIKKKLTLIELHQLFFPYIKELRSIDFESYKVLFIFDGLDECRLPLDFQNNESLWEVTDSASVDVLLTNLIKGNLLPSALLWITSRPAAANQIPPECVDLVTEVRGFSDPQKEEYFRKRISDQSLANKIISHIKSSRSLYIMCHIPVFCWIAVTVLERVLGGAERREFPKTLTQMFSHFLIFQIKHKDQKYHGKYDTDLQQTREMILALGKLAFQQLEKGNLIFYEEDLRECGIDVREVSVCLGVCTQIFREEFKLHLGKVFSFVHLSVQEFLAALYAFLSFISRDGTKQLNRDLSGIFIQYKMSDFLKAVVGKALESKNGHLDLFLRFLLGLSLESNQTVIRALSSVLSSNPSNLRELNLSKNELQDSGVELLSAGLENPHCKLEKLDITDKGFAALASALKLNPYSELRELNLNSNKPGEAGVKLLSDLLEDPHCKLEILHITCEGCASLVSALKSNPSSHLRELNLNYNKPGDSGVKLLSDLLEDPHCKLETLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEN1</accession>
    <name>A0A3B4CEN1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEN1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009336"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015678.2">
        <property type="protein sequence ID" value="ENSPNAP00000009336.2"/>
        <property type="gene ID" value="ENSPNAG00000014854.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166049"/>
    <dbReference type="OMA" id="IFLPCHE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47272">
        <property type="entry name" value="DDE_TNP_1_7 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47272:SF2">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="2">
        <location>
            <begin position="90"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009336.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="519" mass="60348" checksum="8964AE21F010825C" modified="2024-11-27" version="2">MVLLIDLERDNGEVTCMPEELDVVDEVEIEETDSAGTEANSKRTEEDRGGQEEMTVTRKEEIKWRHRPFTTIIDTSFENPPIHEDHPMRPFEYFNQYILDEIFNLMADNTNVYALQSATRCFKPTSSGEIRTLIGLHLAMGVMKMPRVSMYWEAGMDIGIFQNTMPRYRFFQLRSHLHLVNNLEKPAENNDVFFKVRPLYDAIRSRCLELPLEENLCIDEQMVPFRGTLSVKQFIKGKPHPWGVKIYFLCGKSGMAHDFLLHQGTTMELSQQHRKQLGLGVGVVYHLSQRITEANHKLYFDNYFTTKIHAAGTARVSRFARPPLQSDKEMAKKPRGSCDEVVSRDGKVALVKWYDNRPVLMASNFVGVGTMDEVQRWDKKKAQFVMVSRPEVVKLYNDAMGGDDLLDQLVSLYRTEIRSKKWTLRMIKHAFDMAVVNSWLEYRLDAKRRPVCRPFQDVRPLPKVQYDMVDHMPNYDEKKEATRCKMPKCTGKTHVFCDKCNIHLCFVPQRNCFKAAHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIY3</accession>
    <name>A0A3B4CIY3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RING finger protein 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (when phosphorylated) with 14-3-3. Interacts with the E3 ubiquitin-ligases NEDD4, ITCH, SMURF2 and WWP1. Also interacts with the E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2N, but neither with CDC34, nor with UBE2L3. Interacts with ZNF350, EPS15 and STAMBP. After TNF stimulation, interacts with TAX1BP1, TNFAIP3 and RIPK1; these interactions are transient and they are lost after 1 hour of stimulation with TNF. Interacts with GGA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017549032.1">
        <property type="nucleotide sequence ID" value="XM_017693543.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIY3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011330"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018275.2">
        <property type="protein sequence ID" value="ENSPNAP00000011330.1"/>
        <property type="gene ID" value="ENSPNAG00000016982.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425116"/>
    <dbReference type="CTD" id="100150568"/>
    <dbReference type="GeneTree" id="ENSGT00730000110988"/>
    <dbReference type="OMA" id="HLDCIDN"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16468">
        <property type="entry name" value="RING-H2_RNF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000134">
        <property type="entry name" value="Ring finger protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042981">
        <property type="entry name" value="RNF11_RING-H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052804">
        <property type="entry name" value="UEC_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359">
        <property type="entry name" value="GEO07743P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0519">Myristate</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="99"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="154" mass="17343" checksum="243702D4E76DFE86" modified="2018-12-05" version="1">MGNCLKSPTSDDISLLHESQSDRASFGDGTDPEQEPPPPYQEQIPVPIYHPTPSQARLATQLTEEEQVRIAQRIGLIQHLPKGVFDPGSDGTEKKIRECVICMLDFVYGDPIRFLPCMHIYHMDCIDDWLMRSFTCPSCMEPVDAALLSSYETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CL26</accession>
    <name>A0A3B4CL26_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Shadow of prion protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CL26"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012050"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019207.2">
        <property type="protein sequence ID" value="ENSPNAP00000012050.1"/>
        <property type="gene ID" value="ENSPNAG00000017772.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016468"/>
    <dbReference type="OMA" id="ESPDMIG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.790.10">
        <property type="entry name" value="Prion/Doppel protein, beta-ribbon domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036924">
        <property type="entry name" value="Prion/Doppel_b-ribbon_dom_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017400100" description="Shadow of prion protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="15495" checksum="C273425C2D20ABD8" modified="2018-12-05" version="1">MPGCQKLPLVFVWMLLVSCLCPWASCKRGGVFGGRGKGVGMGGKSPPTQSKGSSKQGLKLAGAAAAGAIGGAAIGYGLGSLGRPRYGYGYGYGYDGDSSEEDRRYYHADGPGYYNRSDGRYYRGRSSSGPTLNTLLMIGAVLSIFLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CLE6</accession>
    <name>A0A3B4CLE6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CWF19-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CWF19L1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWF19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CLE6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012170"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019363.2">
        <property type="protein sequence ID" value="ENSPNAP00000012170.2"/>
        <property type="gene ID" value="ENSPNAG00000017936.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156000"/>
    <dbReference type="OMA" id="IVPITHY"/>
    <dbReference type="OrthoDB" id="444325at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061632">
        <property type="term" value="F:RNA lariat debranching enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07380">
        <property type="entry name" value="MPP_CWF19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000024">
        <property type="entry name" value="CWF19-like cell cycle control factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF4">
        <property type="entry name" value="CWF19-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cwf19-like C-terminal" evidence="5">
        <location>
            <begin position="335"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Cwf19-like protein C-terminal" evidence="4">
        <location>
            <begin position="456"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00041007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012170.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="551" mass="62445" checksum="96291BB61BD617EF" modified="2024-11-27" version="2">MEEKPLRVLSCGDVEGRITALFNRVNTIQKKSGQFDLLLCVGEFFGTSPEAEAEWEAYKSGAKKAPIHTYVLGATSQETVKYFPSSDGCELAENIVCLGRRGIFTGASGLQIAYVSGREAQQEPAPAHCFTAKDLTSLLTPLVSSSKFKGVDILLTSQWPRGVWQYGNNPEIDTKFCGVSSVANLAEKLKPRYHFAALEGVHYERLPYRNHVVLQENAQHVSRFIALATVNNPAKKKYLYAFNIVPMKCMDAVELVKQPQDVTENPYRKPLKEGKKYKPPPSAVDEQEEPVSQFFFDLGQKKQQHHRHQQNQGAGRKRQSEGDGSSQPKQPRKPPQPTGPCWFCLASPEVEKHLVISIGTHCYMAMAKGCLTPDHVLLLPIGHYQSVVDLPSDVVEELENYKSAIRKFHKSCGRRCILFERNYRSQHLQLQVVPVPMERCSTEDIKEAFMVQAQEQQMELMEIPEHTDLKQIAPPGTPYFYVELDTGEKLFYRIKKNFPLQFGREVLASEAVLNIPTRSDWRDCKNSREEEEALTKQVRSDFQPFDFTLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNZ6</accession>
    <name>A0A3B4CNZ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017566290.2">
        <property type="nucleotide sequence ID" value="XM_017710801.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNZ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013075"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020543.2">
        <property type="protein sequence ID" value="ENSPNAP00000013075.2"/>
        <property type="gene ID" value="ENSPNAG00000018901.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435176"/>
    <dbReference type="GeneTree" id="ENSGT00940000158367"/>
    <dbReference type="OMA" id="AKMECIL"/>
    <dbReference type="OrthoDB" id="9873136at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000191">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002026">
        <property type="entry name" value="Prostaglandin F2 receptor inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF3">
        <property type="entry name" value="PROSTAGLANDIN F2 RECEPTOR NEGATIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043994379" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="24"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="831"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="23"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="151"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="279"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="410"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="549"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="684"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013075.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="875" mass="96159" checksum="BEAD55CFC5581639" modified="2024-11-27" version="2">MHKEKGVFSLFILFLGLATLCESRVVSVPAGPLVRVEGQSVAIRCNVSDYQGPRDQEFEWALILDNGAEVQLISTFDPLYPDTSVKDRVNSGGISIKKLSDASVELIISKVRASDSATYRCSTPSTDSVMSGNYHADVELRVIGDTLRVVPSIPQPAVSEGEPLELQCNASRAYTAHTFLSITWSIRKETNTLEDILTFGPDSGVKAGQGFAQRYADGGLLLDLREGGFYGLVMKGVKPSDQGAYVCTAREWTRQPGGGKGWHKILEKSEEMGMVTVTPLAQSLVVSVEKNITLNVEDTLNLTCLLAAHGLLSLDLELTWLVRAVNSSAGQRVLIHVGRDGQVLNGSELVGMSRVKPDAFRLILPKVQRSDSGLYFCQVKAWLPQGSGRWYQAAEKTSDPVQVLVTRLDPEFKVTLTASLTPQFTSDPTEMVCQVTNLLNLRDGQLSVSWTYAMNTPGDSSISTIASINEHGVLVPGEKYRQQLDRGDIAVTRSEQNAFRLRMLRTRDEDMGFYSCIVTAWTPSRQAGWDKAKEIKSDPVTVQWKPKTPVLSVVAHRVREASTGGSTFEMSCRVTGQNLQNPGYSVLIRFEDAQGQNPQKILSLNADSVLQLEEGMAASRTDSVALEKMGQQEYRFRLYGVQVSDRGFYCCEVTAWTRDQSSDWSRAVSAESNKIEIAFADSGPVFNVSIHSDRYRVLPGDTAKMECILSTLGATPNTDDVAFDVRWFQSPARAVENGGAVPLISMDRWGVVKKSGGNESTECSLERTDRNTFVLSVHRTQDRDIGEYYCTAKPWYILPDAGTWRDGPELTSAPVILSMKLALWDSLKMPVLYGVVAALGIGALSILLGLLVANCCLSRNPMHTPRSKLMDLEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQN8</accession>
    <name>A0A3B4CQN8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myomesin 1a (skelemin)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017575153.1">
        <property type="nucleotide sequence ID" value="XM_017719664.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000013086"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020587.2">
        <property type="protein sequence ID" value="ENSPNAP00000013086.1"/>
        <property type="gene ID" value="ENSPNAG00000018924.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440660"/>
    <dbReference type="CTD" id="558671"/>
    <dbReference type="GeneTree" id="ENSGT00940000154982"/>
    <dbReference type="OrthoDB" id="8776562at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000069">
        <property type="entry name" value="Alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000029">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000124">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000134">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000192">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000197">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000233">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002172">
        <property type="entry name" value="Myomesin 1a (skelemin)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000179">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF16">
        <property type="entry name" value="MYOMESIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
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    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
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        <property type="match status" value="4"/>
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    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="5"/>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="167"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="276"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="391"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="519"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="620"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="718"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="823"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="911"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="1393"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1209"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <begin position="11"/>
            <end position="22"/>
        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1483" mass="166091" checksum="0345F8C42F40375A" modified="2018-12-05" version="1">MSGSLQVQKQQEQRDLETHFESSYHRSSLSSVSCHSYASHVSSKGVKTTRKKQEKKLSTLSKKDKRPRLAVDKEDEVIGYVVPVFRSSHLATQELMETQEELKEEGVGYVVMRNLFSRDTDYQLRVEKKTHSRSIRESADRLRLGKKIQEKQEFQKKMNPDSLTHPPEFTVKPRGQTVWEGKSVTLHCTVAGWPKPRVAWYKNNVLIDAKAHSKKYTTESNYNIHSLEIKKCDFSDTAEYRISALNVKGESSAFASIIVKRFKDEEVTERPHGYSPEYGVTFRTSIIDKFGVAFGREGETMSLGCTVVIYPTVKRYQPEVLWYRDGVLLTPSKWVHMHWSGDRATLTLVHLNKEDEGLYTLRINTKSGFDSHSAYVFVRDADVEAEGVPVAPLDVRCHDANKDYVVVTWTQPAVEGSSSILGYFIDRCEVGMQHWVQCNDTPVKYARFPVTGLIEGRSYIFRVRAINKAGVSHSSRVSDTVVAMDPSDRARLRAGPSAPWTGMIKFTEEDPTVGVVPGAPTDLAVTEATKSYVVLSWKPPVQRGHEGVMYYVEKCLVGTETWQRVNTGMPVKSPRFALFDLAEGKSYNFRVRSCNSAGVGEPSEETEATTVGDKLDLPSAPGSIVAIRNTSTSVVVTWGVSKEVRNLRYYIEVSVVGSNAWVPCNNKPVKGSRFVCHGLNTDDNCVFRVKAVNAAGYSTYSTESEACVVKPSIEVPSPPSAVTLLERVRDYMVVGWKAPAKTGGADIRGYYLDYRIVKGNVKSKWHEINLKAVTDTTYKVENLKENTFYEFQVRAVNQAGISKGSVPSAAFECKEWTVAVPGPARGLHVLEVRKDSMVLLWEPPTFNGRSPVTGYYVDIKEEKGRWRSIQERSTKNTYLKICGLKEGVSYQLRVHAKNIVGVGGPSQTLGPVVAQTRPGTNEIVVDVDDNGVISMIFECSEMAEGSQFVWSKNYKALTDTSRLTIETVGGKSRAIFNDPSLVDLGTYSCVVSNTDGVSASYTLTEEGLKRLLEISHEHQYPIIPFTSEMAIELQEKGRVRFWTQVAKFTANCQVEYVFNDNIITQGKKYTMNFDKSTGIIEMFMDSLEVTDEGTFTFNLVDGKATGRTSLVLIGAEFAEIQKKSEFERAEWVRKQGPHFVEYLSFEVTPECDVYLKCKVGNIRPGTERVWIKDGIEIEEGDEDAQKIGVAEGVLSFNIGKLVIKSEKEEKKKPAKPAAEEPPRKPKLSKKDAGVYEVELRDERGKDKTLLNLTDAGYQAVLNEMFRVIANSSSELKVESTEHGIILYTNVVYYTEELRVNWLQKDSKISHSDRVQCGVTGEQLWLKIKEPTEKDKGKYAIDIFDGSAGVKRVLELTGQVWEEAYAEFQRLKAAAIAERNRARVVGGLPDVVTIQEGKSLNLTGNVWGDPVPEVSWTKNDKELLPDEHYTLKFEHGKFASITIAAVTTTDSGKYALVVKNKYGKEAGKFTVSVYIPEDEAEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CR92</accession>
    <name>A0A3B4CR92_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CR92"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013880"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021595.2">
        <property type="protein sequence ID" value="ENSPNAP00000013880.2"/>
        <property type="gene ID" value="ENSPNAG00000034102.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182977"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF87">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043702803" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="129"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013880.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="252" mass="27644" checksum="B21D844F3B3D6496" modified="2024-11-27" version="2">MHFYSLLSSSILFVLLLCISDSESLKTLENLTVKCGESLTILCLYDEKYKSNRKYWCRGYLFSSCKIVASTNTSGRTSVTDHPTQNMFTVELKSLQDSDSGHYWCAVEIGGRGTPDDKDYLYLTVSADPAVSVINSTVSGQEGGSVSVQCLISAEYQNKQKNWCRFKDWTCFTVGRTTTSQNSAVLVTDYGKGSVSVEMRGLQKSDAGWYWFSAGDVGVTVHLTVTERPSTTTAVTTVCATEETSISETTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSU1</accession>
    <name>A0A3B4CSU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylethanolamine binding protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PEBP4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CSU1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014445"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037329.2">
        <property type="protein sequence ID" value="ENSPNAP00000014445.1"/>
        <property type="gene ID" value="ENSPNAG00000020353.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426690"/>
    <dbReference type="GeneTree" id="ENSGT00940000162387"/>
    <dbReference type="OMA" id="QKITSWM"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017312010" description="Phosphatidylethanolamine binding protein 4" evidence="1">
        <location>
            <begin position="22"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21833" checksum="ED02EA6387FBE93E" modified="2018-12-05" version="1">MVFAGFCLFVVASVLLTGLTAHSDGLSAEDEAFCRGGLELSYPDLDIKACMVIPQEVREKISKDWGPPKVQLATAEKKKKYTLIMVDPDAPRRNNPTRSYWRHWLLVDIEGSGLRAGDIKGTVLSAYSRPTPPIQTGFHRYQFLLYEQPTDQVLSLSEQEKSSLGNWDPQMFVQKFGLEGPVTSLQFLTQNHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CV17</accession>
    <name>A0A3B4CV17_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ADGRG6</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CV17"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015240"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023386.2">
        <property type="protein sequence ID" value="ENSPNAP00000015240.2"/>
        <property type="gene ID" value="ENSPNAG00000021270.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163009"/>
    <dbReference type="OMA" id="CEVNGAW"/>
    <dbReference type="OrthoDB" id="10002959at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000057">
        <property type="entry name" value="Zgc:100868"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF171">
        <property type="entry name" value="SERINE PROTEASE 56-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043310330" description="Peptidase S1 domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="33"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="327"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="606"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015240.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="869" mass="93529" checksum="5B64B58A5045C0DD" modified="2024-11-27" version="2">MDWVDVLILASLLSHGSYGQLDVCGTTFFNSRIVGGCDAPVGSWPWQASLHYLGEHACGGSLINKEWVLSAAHCFFFTSSSTSSWTVYLGRQNQEGANPNEVSRRVSTIIVHPDYDSNTKDNDVALLKLSTPVSFTSYIRPACLAANNSVFHSGMESWVTGWGNVNEGEPLPSPQTLQEVKVPVIGDRQCRCLYSVEEITNNMICDGLLEGGKDSCQGDSGGPMVSKQNSVWVQAGVVSFGVGCAQPEFPGVYARVSRYQTWISSHISSDPPGFVQFFSSGADPDSTDTCPALPLPVTTGPENSSETNLSPASSAEVCGTTLFSSRIVGGHDATAGSWPWQASLHLGEHFCGGSLINKEWVLSAAHCFEGSSTFEWTVYLGQQNQEGSDPNAVSRNVSVIILHPNYNNDTYDNDVALVKLDSPVSFTEYVRPVCLAAEDSVFNTGTSSWVTGWGEVDQGVPLPSPQTLQEIDIPVIGNRQCNCLYDGGVTNNMICAGLLEGGKDSCQGDSGGPMVSKQNSVWVQAGVISFGVGCAQPEFPGVYARVSRYQTWISSHISSDPPGFVQFLSSGADPDSTYTCPTTASLTTTRALQWRACGEAPLNSRSRGNTTAIDGTWPWMVSLQWDGQHMCSGTLISAEFVMTTALCLSDSPQNASSWTVSLGYQSKRNSSDAFLHSVVVVAITLSNLTHMPNIAVLQLEEPVELSDLIQPVCVDLDSQRSFPLGLTCWVTGYRDGNATGLEVEEQHTTLVDCGGSQSSSEHICTEALFTEEGDLGSVLTCKSGLSWQQAGMIMSVSEGNSIRESRMQVFSRVSEFEDFLQRTVTDLHLYMSKATSAPSIEETTRSQSAASSSSSLFLLSGLLVFCFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVX7</accession>
    <name>A0A3B4CVX7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037402964.1">
        <property type="nucleotide sequence ID" value="XM_037547067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVX7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014694"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037435.2">
        <property type="protein sequence ID" value="ENSPNAP00000014694.2"/>
        <property type="gene ID" value="ENSPNAG00000020706.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438973"/>
    <dbReference type="CTD" id="777608"/>
    <dbReference type="GeneTree" id="ENSGT00940000155128"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF7">
        <property type="entry name" value="FH2 DOMAIN CONTAINING 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="59"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014694.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="827" mass="92431" checksum="0A5255F24F47D4D9" modified="2024-11-27" version="2">MDGVAVVTPPQLSRDPPDFSPPCPNESSSIRPPPPPPPAPAPPPPPPPPPPAPSGGDPFSRTVHRRSRMRNFNWDTIPRHSVVGKRNVWTSQRKLEDFPLDTASIEELFSHSEQQRVPRRGGTVKKSVWGLQSTNQQSDMISIVNSKKSMNIGIFLKQFKRPMQEIVEDVRQGNMRFAAGRLKELSKLLPDDVELKKLLSFSGDVSQLAEADRFLLMLVKIPGYEERLESLLLREEFGPFIEEATNSISVMTEAAGELLTCDDLHSIIRLVLKAGNYMNAGGYAGRAIGFRMTSLLRLVDTKANKPGLNLMHYVAMQAQQIDSALLNFPKQLQHIGEASRIQKQEVEADLQREAEKIKKARDCASKQPDLQCQMEEFLRMADNRLADMEASLQTLDSISDSVAEYFCEDPATFKLEECCSIFHSFCEKFEKAVQENGEREAVEKRRRQQREREVLKRAAKRRSIGTCTSRDSDVDASALESILTSFLTDRPARRRQPSSNRESPTDLLNSNNPLKETSQMALDSPTKTEKKDSDILDGEQLRELDQSFQTKEDTPSAVDNQQTPAGFRRGPCIDNKRTPKSVDRKPSCRKSILTPQNPVLSEEESIDQDGDVQVNSQKEPAQVPEVSRKVLQRQSSRGSLKGDPAPEHPRTYLDTFSSPVHKGMREVELHNGLECLSSPWTVLSPHISPRSTPHRRHSFTLATRDEELDDGVWALPDTPVRGKPPPLVHKCRSYEHSVSTSVLTDTGVRTPHILDCPSGAPLLRSASVGSEAPNSAPGFRLGVLFQRRSQEPLLAKRQEPSALVTFFRRFGERGRPASVGDSHRADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CX95</accession>
    <name>A0A3B4CX95_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GDP/GTP exchange factor Sec2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the SEC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566147.1">
        <property type="nucleotide sequence ID" value="XM_017710658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CX95"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015164"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023288.2">
        <property type="protein sequence ID" value="ENSPNAP00000015164.1"/>
        <property type="gene ID" value="ENSPNAG00000021259.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435092"/>
    <dbReference type="GeneTree" id="ENSGT00940000157998"/>
    <dbReference type="OrthoDB" id="5560525at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4880:FF:000001">
        <property type="entry name" value="Guanine nucleotide exchange factor for Rab-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF2">
        <property type="entry name" value="RAB-3A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25555">
        <property type="entry name" value="RAB3A-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="9">
        <location>
            <begin position="170"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="165"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="482" mass="53678" checksum="E5E4E4466093C054" modified="2018-12-05" version="1">MCFLDVGLIKEQRHWPLNVCDILASCRSAYYDSEAMANEPLEGFHEVNLASPTTPDLHGIADPRPPKPSVPPSTLYRTHSLTSAPGPPNTLYADQLPTQPVYPAPRHLGNDEPQNGSVPGLEPAAPGSASQEVGSVVGVVLGDEGEDPLGLSTDSLSRLRSPSVMEVREKGYERLKEELAKAQRELKLKDEECERLSKVRDQLGQELEELTASLFEEAHKMVREANVKQATAEKQLKEALGKIDVLQAEVAALKTLVLSTSPTSPCKELPSGSRAPFKKGHARNKSTSSAMLGSQQELSLPQPLIRDCKEVDSLLFNEFKAWKEEPTLERTCSFLDRIYREDIYPCLTFSKSELGSAILEAVERNTLSVEPVGFQPLPVVKASAVECGGPNGRRSEIVTKCALSGQTKTCKHRIKFGDSSNYYYVSPFCRYRITSVCNFFTYIRYIHQGLVKQQDAEQMFWEVMQLRKEMSYAKLGYYRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CY50</accession>
    <name>A0A3B4CY50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">VWA5B2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CY50"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015464"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023668.2">
        <property type="protein sequence ID" value="ENSPNAP00000015464.2"/>
        <property type="gene ID" value="ENSPNAG00000021558.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157096"/>
    <dbReference type="OMA" id="EDCCFDC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052627">
        <property type="entry name" value="VWA_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46299:SF2">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46299">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5B2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13757">
        <property type="entry name" value="VIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="3">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="370"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="710"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1048"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015464.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1326" mass="146497" checksum="6495F5ACE3596DA3" modified="2024-11-27" version="2">MPGLRNRATWAPLLLKSSRITSCANGCSLGITAHLTYANTESEPVEGLFVYPLSEREVVVGFEALVSGRLLSVELQSRGKLDDCCLDCCPGSAFNSQCGHSKEWTCCGGNSLDIQCCNGHLLLDEDLERTTFIIGTGLIAPMEMVSVVISTTLELPTLENGAIHLIYPTVLTPIVRARMQPTKSENGGRSEETNATRCFGVSGKQEKLLEVNEQCAHALFSSPATNLAPYELNFQLLVRGACLLAGLESPTHALRADAEPSAQSASATYITLAEDHPYDRHLEIILHLSEPHSPLVILERGRLTFSQYEQQISARRDFIRCVRKEAEPEKKLEFMRKRYHTDVLSNPVLMLNFCPDLSSEPLELQQASRELLFLIDHGANVTQHTINKIKEGMLVAVKSLPHGTMLNVVGFDSSVKPLFASSKPCTDSTLAQVLDYIQRLRADQGATDLLGALSWVYRQPVHRSCPRQLFILTDGSLGSVGRALELVRRNTCNARCFGLGLGPRACRRLLQGIAKMTGGNAEFHCEEERLQPKLIKCLKKALEPALTDVRIDWYVPDNMEALLSPNEIPPLYPGNRLIGYCTLYDVSAFKVKKNEVKSHGYRPAPRGSVGSVFELSPGSSELLQPLVPASEREAAEEGEIEDVLREISQEISSEFSCAPATDNTSNSVTDQDYIVSDVRWRMEQASYVQEQYMLTRCSLSSERGLASFSSPSEGLSLSMDTGSLPQGLERVQPQGRGLSRWESPWQQTATSSVDTEPEKGVRLSDREECVRRQKALARCALASRSFSSPQGDLDMHCLRRALERVSFEQTVGGKLDENDIETHNTPQALSLTDSNGLLFPASPLDWDSFTDPEALFAPTPAGDSQAGQASCRSVIHGLLGARPVSWEVAVDLSTLWATESPPRGHWDEIIHQLTARSIIRDFENMAEKETDIEHGSYKRYRMKAIQTSKSCGIICMYTAFTTTDTSPKKTLSEATNSRYTGVRFGSRRSTSSKRQRTLSVGFGRRPSNRDSEEIDDIHTSTDRDNTPASPRSLASWDSSVGRNVCPGSPSAASSRSQRSIESRSVESFFTTRFNLGRLRSSHSSGRQTPLKPQCLSAESDTYTDSISPDSLPLVRLQLASGAFLLCESFSECIHIPLDRLKRASPYLSHRSSLSPPYRCISPPGTRPIAPYRPMDHTSTHQPHASLRRYYDNEPLTVFSDPEEGSPEPVGGILQADSGRGSETDMCEGSPLEGVDPHGEELAQLDVESSSWATALALAWLEHHCAGYFVEWELVAAKADFWLKCQSLPEGVDLPGLRAAARQLFLLLRHWDENLQLNMLCYNPNNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYE1</accession>
    <name>A0A3B4CYE1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYE1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016370"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038090.2">
        <property type="protein sequence ID" value="ENSPNAP00000016370.2"/>
        <property type="gene ID" value="ENSPNAG00000022504.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010961"/>
    <dbReference type="OMA" id="HNRFSTM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="41"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016370.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="237" mass="27557" checksum="95705B5D786B1660" modified="2024-11-27" version="2">HFPLLYCGGAVKDLRPDVSDQELFWGTDQYDFAIVLPASGLECFWHFAHQGERFYLDFMVQWVTGVANDRHLSVTVNAPSGLIVGSADDAKGQIAFNAGETGFYQMCFSNFHNRFGSMQIFLNFGVYYEDSDDVKNQKEEEEIKKEQDRKHLNDTLTTIEESSSRVQGFVFHMWRYYNFERMRRGADYYLLQSNSNYVSTWSVVQSFVIIMAGYLQLFFLKKLFDTKTTTETDKPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYR8</accession>
    <name>A0A3B4CYR8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">EXOC8</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the EXO84 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575954.1">
        <property type="nucleotide sequence ID" value="XM_017720465.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYR8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017237"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025962.2">
        <property type="protein sequence ID" value="ENSPNAP00000017237.2"/>
        <property type="gene ID" value="ENSPNAG00000023463.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441123"/>
    <dbReference type="CTD" id="149371"/>
    <dbReference type="GeneTree" id="ENSGT00390000015936"/>
    <dbReference type="OMA" id="AAWLPNR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01226">
        <property type="entry name" value="PH_RalBD_exo84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1210:FF:000001">
        <property type="entry name" value="exocyst complex component 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1220:FF:000002">
        <property type="entry name" value="Exocyst complex component 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000180">
        <property type="entry name" value="Exocyst complex component 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25345">
        <property type="entry name" value="PH_EXO84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="148"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="663"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017237.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="684" mass="78837" checksum="23AC4AAF66B6BF09" modified="2024-11-27" version="2">MAETGSRLRKQLESANFDPQNYVKQLSQQSDGDRDLQEHRQKIQSLADETAQNLKKNVYKNYRQFIETAKEISYLESEMYQLSHILTEQKSIMESITQALLSTDKDETAKEMLTAFPKETEEVKQRTLTTLLEKVEGCKNIMETPGRYLVYNGDLFEYDADNMSQIQKVHAFLMNDCLLIATWLANRRGTVKYKYSALYDLESFAVVNVKDNPPMKDMFKILMFPDSRIFQAENSKIKKEWLEILEETKKNKVAKEKHKKEEEVPTSPVRPEVSMNPFDEEEPLDSEELVDLSPEWIQELPEDLDVCIAQRDFEGAVDLLDKLNEFLKDQPVTPRVKELRGKVDERVRQLTEVLVFELSPDRSLRGGPKATRRAVSQLIRLGQSTKACELFLRNRAAAVHTAIRQLRIEGATLLYIQKLCNIFFTSLLETAREFETDFAGNTGCYSAFVVWSRSAMKMFVDAFSKQVFDSKESLSTAAECVKFASEHCRQLSEIGLDLTFIMQSLLVKDIKASLLSQKDIIIEATKHRNSEEMWRRMNLMTPEALAKLKDEMRSCGVGTFEQYTGDDCWVNLSYTVVAFTKQMMAFLEEGLKLYFPELHMVFLESLREIILVAVQHVDYSLRCEQESEKKAFILQNASFLHETVLPVVERRFEEGVGKPAKQLQDLRKSSRPVRVNPESTMSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D418</accession>
    <name>A0A3B4D418_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM37</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D418"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026516.2">
        <property type="protein sequence ID" value="ENSPNAP00000017634.1"/>
        <property type="gene ID" value="ENSPNAG00000023913.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006225"/>
    <dbReference type="OMA" id="WCEFIAT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005244">
        <property type="term" value="F:voltage-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029372">
        <property type="entry name" value="Tmem37"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31767">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-LIKE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31767:SF0">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-LIKE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15108">
        <property type="entry name" value="TMEM37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="23975" checksum="E2F3433E7F1027A3" modified="2018-12-05" version="1">MTAIKMRAVAPAAQGKSRPLFLEVLCRSLIILCVALSVVLSSVAVCDGHWLLSDRHMFGLWFFCVVEDSGAAPNCSRHVGGGLDPGLCVCRSVVSLAVVSAIFGLELLVMSQVSEGRASSQRWHLGAWLVLLAAGLAAGGVATFVFLFWEHATPLGFTLTFWCQFTATFLFFLNGMAARHIQNMAELLPSSGHLGKPYLATLQTMMMSTGVPKWTGQELELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4T3</accession>
    <name>A0A3B4D4T3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP-binding protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GTPBP8</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558547.1">
        <property type="nucleotide sequence ID" value="XM_017703058.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D4T3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018545"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027791.2">
        <property type="protein sequence ID" value="ENSPNAP00000018545.1"/>
        <property type="gene ID" value="ENSPNAG00000024943.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430516"/>
    <dbReference type="CTD" id="29083"/>
    <dbReference type="GeneTree" id="ENSGT00390000001083"/>
    <dbReference type="OMA" id="RMDHAPP"/>
    <dbReference type="OrthoDB" id="391988at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000857">
        <property type="entry name" value="GTP-binding protein 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052279">
        <property type="entry name" value="EngB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498:SF1">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EngB-type G" evidence="8">
        <location>
            <begin position="102"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="283" mass="31685" checksum="2584FF01C485E2D9" modified="2018-12-05" version="1">MSAQVLLRFRSAAGPVSRCHAHQLASVSQAARLSEKKLKGLLYPFSDLEAHLSRQVDQRRFRLFQPNMENLERAEKLFIPSPKHHILYSTSAVRAVHMPELAQPEVCFIGRSNVGKSSLIRALFSLAPEVEVRVSKTPGHTRTLNFFTVGKAFTLVDMPGYGHRAPKDFVDMVEPYLQERQNLVRTFLLVDGGAGLQKLDLAAVEMCEEFGIPYVLVVTKIDRTRQGALLTLLLQLHDFIKDQTSTCFAQPFIVSSVQYSGVHLLRCFIAHVTGNLQLSSKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6Q5</accession>
    <name>A0A3B4D6Q5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017568738.1">
        <property type="nucleotide sequence ID" value="XM_017713249.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6Q5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020047"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030288.2">
        <property type="protein sequence ID" value="ENSPNAP00000020047.2"/>
        <property type="gene ID" value="ENSPNAG00000004105.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436628"/>
    <dbReference type="CTD" id="562442"/>
    <dbReference type="GeneTree" id="ENSGT00940000158272"/>
    <dbReference type="OMA" id="QYNDGFP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF49">
        <property type="entry name" value="COLLAGEN ALPHA-1(VIII) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043556987" description="C1q domain-containing protein" evidence="7">
        <location>
            <begin position="34"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="8">
        <location>
            <begin position="607"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="103"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="275"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="401"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="558"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020047.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="740" mass="72583" checksum="3FFBE08BED223A73" modified="2024-11-27" version="2">MEPKSKTDIAMPLHFSHCFAFVVQLYLLHHAQAGAYYGHKQGPQQLPQQHHPLPQLPHIPLGKEGIPQQQYLGKKPHLPYGNEMPMLPHYGKEQQQVPLQNLGKGETIPRGEKGLPGEVGPMGPPGPQGPPGLPGQGIPGQPGKPGPPGPQGYPGIGKPGMPGPPGKPGGIGLPGQRGELGPSGGAGPVGLPGPPGLPGPPGLPGLVNAGRPGLPGQPGIRGEPGQKGPPGFSGIPGLKGDKGIGLPGLPGVKGPPGLPGPPGPGGLPGVGKPGLNGLPGVPGAPGKQGPPGELGLGGLPGDEGQPGPPGLPGIGKPGLDGLPGKPGLPGGKGERGPPGFPGGPGLPGFGKPGYPGPKGDKGHGGLPGPQGPKGEKGHGGLPGIIGAPGPGGVPGPPGLMGPPGGIGFPGPKGEGGAVGAPGPIGGKGEPGPPGLPGKPGLLGESGQPGPRGFPGPLGPQGENGHKGLPGLPGAPGKPGQKGEIGLPGEGGHQGPIGIPGLMGPSGPIGPPGIPGPKGEYGPPGKPGQPGEGKPGLPGPMGPPGKAGFGGLPGKPGMPGQPGPPGPPGPPAKPPGAGQILPEVGPGLDGAKSAYKKSKLGGHIIGRNGLEMPAFTAQLTTPFPPVGSPVVFKKVLYNGNQNYNPQTGIFTCSMPGIYYFVYHVHCKGANVWVALQKNNEPVMYTYDEYKKGFLDQASGSAVLPLKSGDTVHIQLPSDQAAGLYAGQYVHSSFSGYLLYLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7P4</accession>
    <name>A0A3B4D7P4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Claudin-34</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7P4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018964"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028476.2">
        <property type="protein sequence ID" value="ENSPNAP00000018964.2"/>
        <property type="gene ID" value="ENSPNAG00000025474.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005717"/>
    <dbReference type="OMA" id="MMLAAIF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018964.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="22780" checksum="38F0FCFCAD62F2D7" modified="2024-11-27" version="2">MAFTAKKAHWQFLGLVLAVLGLMLTAGTSGVNNWRLWYVDDLSVISSGVAWVGIWRACFYSHVLDTFEFCQSISITDSFIPPEIVAAQVLCTTAIPIGIAGNLIAGYAVRHVYFNVNRGHIRWAFVLAGALHLLTATCSLVPVFWNMNSVLKNRTIDFPPEFHFPPAPYKQELGMGFIIGICSSVLLIVSGLLFLSYRSLSGPLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D859</accession>
    <name>A0A3B4D859_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537154.1">
        <property type="nucleotide sequence ID" value="XM_017681665.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537164.1">
        <property type="nucleotide sequence ID" value="XM_017681675.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537173.1">
        <property type="nucleotide sequence ID" value="XM_017681684.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537182.1">
        <property type="nucleotide sequence ID" value="XM_017681693.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037402834.1">
        <property type="nucleotide sequence ID" value="XM_037546937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D859"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019700"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029722.2">
        <property type="protein sequence ID" value="ENSPNAP00000019700.1"/>
        <property type="gene ID" value="ENSPNAG00000026354.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410538"/>
    <dbReference type="CTD" id="7179"/>
    <dbReference type="GeneTree" id="ENSGT00940000154335"/>
    <dbReference type="OMA" id="EFAVEIY"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14510">
        <property type="entry name" value="PTP_VSP_TPTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000053">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TPTE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1110:FF:000004">
        <property type="entry name" value="Voltage-sensor containing phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045102">
        <property type="entry name" value="PTP_VSP_TPTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF60">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE TPTE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase tensin-type" evidence="11">
        <location>
            <begin position="192"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="277"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="12">
        <location>
            <begin position="375"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="511" mass="58059" checksum="CE28E2D6FF5BF56A" modified="2018-12-05" version="1">MSSVHFHPGLDSKDVNGNGMMEEAQVQIDDGKEGSYEPDTLYYRIRKRIAPFVMSFGFRVFGVVLIIVDVVLVIVDLSLPGDSSAVRTPLEAISLVISVFFVIDVLLRIYVEGFKVYFSSKLNVMDACIVIITLVVTVVYTFSSFSGADLIPRLVSILRALRIIILVRVFRLASQKKELEKVTRRMVSENKRRYQKDGFDLDLTYVTDRVIAMSFPSSGKQALYRNPIKEVARFLDTKHPDHYRVYNLCSEKGYDPVFFHYRMERVMIDDHNVPSLEDMLSYTASVREWMAADPLNIIAIHCKGGKGRTGTMVCTWLIDSDQFESAQDSLDYFGERRTDKSMSSKFQGVETPSQSRYVGYYEIMKNSYDRQLPPPKSLKIKSLRIHSIAGVGKGNGSDLKVRIIVKKEQVFQCVCAKQENCALFPDVGSNAVVISLQEGPVVCGDVKIMFESSAGLPKGYEDCPFYFWFNTSFVENNKLYLSREELDNPHKSKTWDIYKEDFGVTLSFSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DB50</accession>
    <name>A0A3B4DB50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Caldesmon 1b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CALD1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DB50"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033071.2">
        <property type="protein sequence ID" value="ENSPNAP00000021617.2"/>
        <property type="gene ID" value="ENSPNAG00000028724.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153901"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006018">
        <property type="entry name" value="Caldesmon_LSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18949">
        <property type="entry name" value="CALDESMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18949:SF0">
        <property type="entry name" value="CALDESMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02029">
        <property type="entry name" value="Caldesmon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021617.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="58625" checksum="DA92D34B37DD5650" modified="2024-11-27" version="2">MAFRRNEEDEEEAARERRRRARQERLRSKESEDAPNPPESVELHNSHSVTVTETVSSSVVSSSQAGEGDDEEQALLERLAKREERRQKRMKEALDRQKEVESTDSSHYSIEEHSSTRRTFHHGLEEQPAVNSKEQEKEKAEEEEAAQEEEEEQKEEEEVVEEKPRRSYLREQMEDEDVGRKSKQQNGGVYEDSKHKKVERTQSRGSLRSPEVPAAEDLDSEGARLEAERKLEELKRRRDETESEEFERMRQKQQEAEAELEELKRKREERRKILEGEEKQRKQEEAERKAREEEEKRRMKEEIERRRAEAAERRQKVEEGVDGDTRKPFVCGRSFSCTSHSPVVSKIDNRLEQYTSAVQTNKDARSPRAAAIDLPTVSDGIRSIKSMWEKGNVFSSSTSTPSSNKDAAGIKVGVAGRINDWLNKTPETGKTSGGRPADLKPGDVTGKRSLWENKGSSATKVTNLKTVAFVVTFVEMFFVLKISFHITQETNCKVFLYPKNSWHVFITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBF3</accession>
    <name>A0A3B4DBF3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CPO</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBF3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020778"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040204.2">
        <property type="protein sequence ID" value="ENSPNAP00000020778.2"/>
        <property type="gene ID" value="ENSPNAG00000027741.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161508"/>
    <dbReference type="OMA" id="SSCDWAG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF19">
        <property type="entry name" value="CARBOXYPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043702807" description="Peptidase M14 domain-containing protein" evidence="10">
        <location>
            <begin position="16"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="11">
        <location>
            <begin position="49"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020778.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="377" mass="42625" checksum="ECCEDEBDF094186D" modified="2024-11-27" version="2">MFFMVLLSQHTTCFMFSITAQLQDCSCKLRTTPSTSPWRRLFTNFFPHSFMNLPQKILILGWMEQMEKNHPDVVSSVMYGMTHEKRNITLLKIGLNSTLPKKAIWMDCGIHAREWIAPAFCQHFVKEILGSYKTDPKMNELFKNLDFYITPVLNMDGYIYSWTRLWRKSRSPGSDGCTCFGTDLNRNFYANWGMVGISRNCCSEIYNGHSPLSESEAVAVTDFLSANRDQLLCFLTIHSYGQLILVPYGHPNISAPNYDELMEVGLAAAKAIKAVHGVDYTVGTPPDVLYANSGSSRDFARLIGIPFSFTFELRDKGDFGFKLPEDQIQPTCEEAYQGAMSIINYVHDKTFPSAAFTVTATLWTTLTAAWLSSTTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCT8</accession>
    <name>A0A3B4DCT8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCT8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034310.2">
        <property type="protein sequence ID" value="ENSPNAP00000022237.1"/>
        <property type="gene ID" value="ENSPNAG00000005002.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002361"/>
    <dbReference type="OMA" id="AVHTQCT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35967">
        <property type="entry name" value="UPF0450 PROTEIN C17ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35967:SF1">
        <property type="entry name" value="UPF0450 PROTEIN C17ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="116" mass="13540" checksum="9E295DD0DAE4E243" modified="2018-12-05" version="1">MIRKGIRLVTLLVNSDGFYKMSRLYVTPDTYFFKVNVLVLDTFKCTKPCPDFKLGSRYIVMGQIYHRRRHLPSSLLALVGGKLKPGDGLLRSSNYVKRYNKRRHQKAQEATRSRCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEG8</accession>
    <name>A0A3B4DEG8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sarcolemmal membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Associates with the striatin-interacting phosphatase and kinase (STRIPAK) core complex, forming the extended (SIKE1:SLMAP)STRIPAK complex. The (SIKE1:SLMAP)STRIPAK complex dephosphorylates STK3 leading to the inhibition of Hippo signaling and the control of cell growth. May play a role during myoblast fusion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer. Interacts with myosin. Interacts with SIKE1 and both associate with the STRIPAK core complex composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26. Interacts (via FHA domain) with STK3 (when phosphorylated); the interaction associates STK3 with the STRIPAK complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
            <location evidence="15">Sarcolemma</location>
            <topology evidence="15">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Mitochondrion membrane</location>
            <topology evidence="13">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the SLMAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037388085.1">
        <property type="nucleotide sequence ID" value="XM_037532188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEG8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021451"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032764.2">
        <property type="protein sequence ID" value="ENSPNAP00000021451.1"/>
        <property type="gene ID" value="ENSPNAG00000028730.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431613"/>
    <dbReference type="CTD" id="572011"/>
    <dbReference type="GeneTree" id="ENSGT00940000157660"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900825">
        <property type="term" value="P:regulation of membrane depolarization during cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21911">
        <property type="entry name" value="CC1_SLMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22679">
        <property type="entry name" value="FHA_SLMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000003">
        <property type="entry name" value="sarcolemmal membrane-associated protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF22">
        <property type="entry name" value="SARCOLEMMAL MEMBRANE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="822"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="22">
        <location>
            <begin position="28"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="464"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="265"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="546"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="642"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="747"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="849" mass="96964" checksum="107F7B956E36101E" modified="2018-12-05" version="1">MPSALAIFTCRPNSHPFQERHVYLDEPVKIGRSVARCRPAQNNATFDCKVLSRNHALVWFDHKTGKFYLQDTKSSNGTFINSQRLSRGSEESPPCEVLSGDIIQFGVDVTENTRKVTHGCIVSTIKLFLPDGMEARRRSDVIQTPLPLPVDKVAANTPSMYSQELFQLSQYLQEALHREQMLEQKLATLQRLLASTQEASESSWQALIDEDRLLSRLEVMGNQLQAYSKNQTEDGIRKELVALTEDKLNYETTAKESLRRVLQEKIEVVRKLSEVERSLSNTEDECTHLKEMNERIQEELRELANKYNGAVNEIKDLTDKIKLAEGRQEELTQKGQNEKKELQLRIEEMEEKEQALQARIEALQADNDFTNERLTALQVRLEQLQEKSIKENNSLDHFLLKSGGDCTLIQQFIECQPVKQLKGAVDSSIHKLSNFDDVIDAHLQNNQTTDDNSLTSPDKVKENQIDAKESDMSDTLSPSKDKSSDDTSDGQMDEQELNEPQNRVSLLKDELQRANLEPGDTEQVIHHLHRELLEAQELANTGKQKCLELQALLEEERRTNRQQTEESAKQIQYLQSQLAKLQSDMEALREQRENTISSTREELYSAQEEVLVLRHAMEAATAEREREIAALQGDLCAVTAELDKWRKAAADYEQEISTLQASFKLQSQHQERAVQLQGELEKLQSECLNLQKECESLRSEKVALLEKLQRLELELDSSREQSATLSCSLNALEKSQGDLESKLGSMQDQHQQDASKLKVQLAQAENRTRSLQKEYEDTQVQLSDLRQRYERTEQEKRSINDELEQCKVNLKLLQEKGKNSGWMPWMPVVAVMVALTAVVLYPSLSKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFJ2</accession>
    <name>A0A3B4DFJ2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017546559.1">
        <property type="nucleotide sequence ID" value="XM_017691070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFJ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022228"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034234.2">
        <property type="protein sequence ID" value="ENSPNAP00000022228.1"/>
        <property type="gene ID" value="ENSPNAG00000006888.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423637"/>
    <dbReference type="CTD" id="100003938"/>
    <dbReference type="GeneTree" id="ENSGT00940000156820"/>
    <dbReference type="OMA" id="SENCKDX"/>
    <dbReference type="OrthoDB" id="5987010at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22578">
        <property type="entry name" value="Mint1_CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06720">
        <property type="entry name" value="PDZ1_APBA1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06793">
        <property type="entry name" value="PDZ2_APBA1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01208">
        <property type="entry name" value="PTB_X11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000044">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000007">
        <property type="entry name" value="Amyloid beta A4 protein-binding family A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000017">
        <property type="entry name" value="Amyloid beta A4 protein-binding family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051230">
        <property type="entry name" value="APP-Binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345:SF14">
        <property type="entry name" value="AMYLOID-BETA A4 PRECURSOR PROTEIN-BINDING FAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345">
        <property type="entry name" value="SYNTENIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="953" mass="106502" checksum="8EC7C10EC31C12D4" modified="2018-12-05" version="1">MSHQEAPLSQELSEVAEADLERRGPEGQQPPSHRRRHDNSGRSRNHHNGHGGENVHGQHGGQRYRRYEGSEGAGRPRSSRRTPEECDLEQQHVQQPIPRPAVTRYRRQGDAEARLRAQQQRQRQRERQQREAERAEQQRLEQQQPGEPEEDEEGGNVLARSASTESGFHNHTDRAEGDAVMALEAEPEDEGTYGIPLRPEAEEYTESAESEQQPAGPPHAQPHYPPQPPPLPREPLPNTERPPRHDAEALNPNYSNYVYTQPLYRGPGREGQAQAEDEPYSEPYADYAVQEHVYEEIPEAPGAQAEGQTPQQRQRAGFRLFERDSYRQQEAVGSRLHHYDERSDGESDSPEKEAEFAPYPRADSCEQDEDIDQIVAEVKESLSSQSLEHAAESTGREEEEEDEWPESEHQEQTETKEGKLQEPPGEEGKGEEGRTPEGSPSEEQAPARSSRQKRDAISLAIKDIKEAIEEVKTRTVRSPYTPDEPKEPIWVMRQDLSPTVESDLQPSLGGDSPGSGSTSPQGAESSSRPLPLQEGNDSFESPSPASKESRRSLAAFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKTAQKLAKNRKKAPEGEAQPMTEVDLFISTQRIKVLNADSQETMMDHPLRTISYIADIGNIVVLMARRRMPRSDSQENMEASDPAQDEKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVYIEKQKGEILGVVIVESGWGSILPTVIIANMMHAGPAEKSGRLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRIKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGL1</accession>
    <name>A0A3B4DGL1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylinositol N-acetylglucosaminyltransferase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PIGC</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017543163.1">
        <property type="nucleotide sequence ID" value="XM_017687674.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGL1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022236"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034309.2">
        <property type="protein sequence ID" value="ENSPNAP00000022236.1"/>
        <property type="gene ID" value="ENSPNAG00000029579.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414769"/>
    <dbReference type="CTD" id="5279"/>
    <dbReference type="GeneTree" id="ENSGT00390000005496"/>
    <dbReference type="OMA" id="STSYHAF"/>
    <dbReference type="OrthoDB" id="196709at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009450">
        <property type="entry name" value="Plno_GlcNAc_GPI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06432">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016104">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="32424" checksum="D294270F4E191737" modified="2018-12-05" version="1">MGADSGPGAPPTVPWRKVLYERQPFPDNYVDRRFLEELRRNIRVRQYRYWAVVREAGLVAQQLSCVAVFLTLWFYMERGDLGPSTLLWASLGFALLGYGLYEVLGGGSRERTRLADLQSAAVFLAFTFGFSPVLKTLTESVSTDTVYAMSAMMLLAHLVSFPYTQPSPPGSLSLNAALFGSVCLASRLPGALHTFAMLSCALLVFALWPCLLHRLRDRAEWSFPCAAALVCVAGVRGLASFWPEGALLLFLALLVLTLACPLLLVHLQRHKDNIHGPWDEAEIREDLSRFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTZ8</accession>
    <name>A0A3B4DTZ8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Low density lipoprotein receptor class A domain containing 4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PMEPA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037392583.1">
        <property type="nucleotide sequence ID" value="XM_037536686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTZ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026930"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003885.2">
        <property type="protein sequence ID" value="ENSPNAP00000026930.2"/>
        <property type="gene ID" value="ENSPNAG00000012292.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410774"/>
    <dbReference type="CTD" id="556780"/>
    <dbReference type="GeneTree" id="ENSGT00390000000724"/>
    <dbReference type="OMA" id="MQNITAP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070412">
        <property type="term" value="F:R-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043445">
        <property type="entry name" value="TMEPAI/LRAD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16514">
        <property type="entry name" value="LOW DENSITY LIPOPROTEIN RECEPTOR CLASS A DOMAIN-CONTAINING 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16514:SF4">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR CLASS A DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="215"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="267"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026930.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="308" mass="34041" checksum="8C6911DEF07FA6F0" modified="2024-11-27" version="2">MQEADLPATNAFTECKFLCTNGRCLDLGSLVCDQLNHCGDNSDEENCPVSTQHPPPAILYSELEFVQIMIIIVVMMVMVVVIICLLNHYKLSTWSFMSRPGQARRQEHSLQPEGCFWPSDSGSRQGASEVVYAPQPRERFSAPSFMQRERFSRFQPTYPYLQHQIDLPPTISLSDGEEPPPYQGPCTLQLRDPEQQLELNRESVRAPPNRTVFDSELIDAPRPPSSNSGISAANSSTRGRMEGPPPAYSEVMGHYPGAAFFLHQHSNNQSSSVSGIQTSPPMGRTGQGSPQSTVVPSKAKDMQTGDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0K3</accession>
    <name>A0A3B4E0K3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Obscurin-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Acts as a regulator of the Cul7-RING(FBXW8) ubiquitin-protein ligase, playing a critical role in the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. Required to localize CUL7 to the Golgi apparatus in neurons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 3M complex, composed of core components CUL7, CCDC8 and OBSL1. Interacts with CCDC8. Interacts with CUL7; the interaction is direct. Interacts with FBXW8. Interacts (via N-terminal Ig-like domain) with TTN/titin (via C-terminal Ig-like domain); the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000029243"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035430.2">
        <property type="protein sequence ID" value="ENSPNAP00000029243.2"/>
        <property type="gene ID" value="ENSPNAG00000015299.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156702"/>
    <dbReference type="OMA" id="WEKPVVF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050775">
        <property type="term" value="P:positive regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001811">
        <property type="entry name" value="Obscurin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002420">
        <property type="entry name" value="Obscurin-like 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001084">
        <property type="entry name" value="obscurin-like isoform X3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001752">
        <property type="entry name" value="obscurin-like isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002120">
        <property type="entry name" value="obscurin-like isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000211">
        <property type="entry name" value="Obscurin-like protein 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000241">
        <property type="entry name" value="obscurin-like protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000502">
        <property type="entry name" value="obscurin-like protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000393">
        <property type="entry name" value="Putative obscurin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000464">
        <property type="entry name" value="Putative obscurin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052385">
        <property type="entry name" value="Obscurin/Obscurin-like_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971:SF5">
        <property type="entry name" value="OBSCURIN LIKE CYTOSKELETAL ADAPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971">
        <property type="entry name" value="SI:DKEY-31G6.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="14"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="118"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="223"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="312"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="493"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="685"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="843"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="935"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1026"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1118"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1212"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1610"/>
            <end position="1698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029243.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1698" mass="191013" checksum="91FE1D2EB2D735A5" modified="2024-11-27" version="2">EKLSSGMDVFGGAPRFLAYPRPVVVQSGADAVLKCQIGGDPRPAVIWERNNEKIHPGGRYRVFEDGNVYNLIITLVTAEDSGQYICKAKNSIGETYAAATLKVEGEAQEMELREENKPRFLIKPLSTRVGRGEDAVFSCKLWGNPRPEVVWEKDGKKLNEIFESTHFSVGYQDGGWFQLKIFKTRAPDGGVYTCKARNEFGESLAGAVLLVDAGPGHEDEGNPKVKMFAVTEGKHAKFRCYVTGKPKPEIIWRKDGRLILSGRRYLLYEDREGYFTLKVLYCKQQDNGVYVCAASNTAGQTLSAVHLSVKEPPIRFKQPLSDLQVWERDLAILECEVPEDSVPITWYLEDRRLQPGAKYGMEEWGTKRRLTIRDIGIDDDGIYLCEMADGGRSIAEVAVKGTIVRKLPRKVDVLEGENAAFCVEVEEEEMDIHWYKDAVELRETHQTILKSFGRTHILVFVNTTPQDSGMVTFFVGRSKTSSQLRVKAARHCPPSCPIGVQINTERANAALLSWVPAQDSRKNPPSGYILERQEMGSQEWLQCLTTDSATSVEILGDSVPCEADYRFRICSINKYGRSGYVEFPRAVHLVPVAKIQAPLQDALVPEGQDASFSMELSASVIGTWFLNGNQLQEDERFSIRRSRTHQSLRIRGVRDTDNGAEITFIAYGIRDSAALYIQEIINQSPLVKFTPLSEMDRNKFVEVVLQCEIADPEAPVSWYKDGIQLQTKNGLVIQSEDRIRRLVVPTAELCHSGVYNCEAMDDVIKFKVYVKGDLKIKPRGGIMLHKNSSGEPFELQCEVLDAAAQVCWYKDGHELQTQAGVLILSEGAIRKLRVQSAELAHAPPVTFVYLPEEELHRNIMEQDSLFLCCEVSRSDATTQWYKDGVEVQPSDKVLIEAENTMQKLIIQSVQLSDAGVYTCRAGDNALIFKVNVREPPVMIVYPKEDVHLDRHVPEEIVLSCELSRPNGKVTWLKDGQKLQESENIKLKTEGPYRRLKILHSRVEDSGEYVCDTADDSKFFYLSIKEPPVRIVSPSQSQMELCQQTSERMVLSCEISRPNASVRWYRDGLEVEENNNLILEVDGVYRRLIIPETTVSDSAEYVCDTADDSVTFFVNIAEPPIKFLQKADISTDIHVVISDAIVLKCELSRANGNAFVQWLCDGRVLQADSRTHIESRGTMRKFVLSRLDIFDSGEYICDANNDKMTTIVTVQAPRIVEFIAELHNVTVMEGEDATFKCVVSPEDAELVWSMNGWPIKSNERFNISSNGLCHMLHITNCQVSDSCRLTAEAEGVISKAILQVEEAQVLFTKKMEPVLAEEFGDAILEVEVSLESGEVQWMRQGVVIHPGPKFTLKQSGQKRSLTIHKLALSDRGMYSCETLHDRTQAKLSVERESARAAAFLAMRCYLFTMVSGDAGPHCHSSHNCCFHVHTARKIKIRKGLSEIQTFERETASFEVELSHSNVEGAWQKDGSRLKNNNHWRMTAKGRVHSLTISNLSLEDTGTYTFSAENVRSSARLIVKERELEVLQSLEDLDIQEDQNAVFMCEVSLDDVPGEWFKNGEKIKPTSTIKIRQEGTKHFLLMCNVKGEDSGEIKFVAKNVESIAYLEVEELPVSIVKPLQDKTALEKSRVILDCTVSNPRCSIRWYKGRNVILPSERFEICSEGCYRKLVIQQVALEDEGTYSVQVGDYTCSARLTVEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2U2</accession>
    <name>A0A3B4E2U2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Complexin 3b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572683.1">
        <property type="nucleotide sequence ID" value="XM_017717194.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2U2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030927"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019436.2">
        <property type="protein sequence ID" value="ENSPNAP00000030927.1"/>
        <property type="gene ID" value="ENSPNAG00000017962.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439029"/>
    <dbReference type="CTD" id="794623"/>
    <dbReference type="GeneTree" id="ENSGT00950000182938"/>
    <dbReference type="OMA" id="ERDARFM"/>
    <dbReference type="OrthoDB" id="9942329at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF5">
        <property type="entry name" value="COMPLEXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="14"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17790" checksum="A3AE130EEF027C9D" modified="2018-12-05" version="1">MAFMVKHMIGGQLKDLTGGLTEEKPEAEKGEAGAKGMTQEEYEQYQQQLEEEKQERDANFAQKKAERATIRTHFREKYRLPKNELDETQIQAAGQDVELPTELAKMIAEDNQEEKHKQSVLGQLSNIQNVDIDHLKDKAQATLEELKNSAEKCSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3I7</accession>
    <name>A0A3B4E3I7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017552982.2">
        <property type="nucleotide sequence ID" value="XM_017697493.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552983.2">
        <property type="nucleotide sequence ID" value="XM_017697494.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552984.2">
        <property type="nucleotide sequence ID" value="XM_017697495.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3I7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030258"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018139.2">
        <property type="protein sequence ID" value="ENSPNAP00000030258.2"/>
        <property type="gene ID" value="ENSPNAG00000016923.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427397"/>
    <dbReference type="CTD" id="405763"/>
    <dbReference type="GeneTree" id="ENSGT00940000165921"/>
    <dbReference type="OMA" id="IFGEPNH"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086013">
        <property type="term" value="P:membrane repolarization during cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086091">
        <property type="term" value="P:regulation of heart rate by cardiac conduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060307">
        <property type="term" value="P:regulation of ventricular cardiac muscle cell membrane repolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000020">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000011">
        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF638">
        <property type="entry name" value="ERG K+ CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="607"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="710"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="218"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="837"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="899"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1062"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="850"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="862"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1089"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1101"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1139"/>
            <end position="1150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030258.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1194" mass="133709" checksum="D4C55C37ECA8A9DE" modified="2024-11-27" version="2">MPVRRGHVALQNTYLDTIIRKFDGQNRKFLIANAQMKNCGIIYCNEGFCQMFGFSRAEIMQQPCTCQFLVGPGTMKSALSQLAQALLGSEERKVEILYYSKEGTCRPCLVDVVPVKNEEGVVIMFILNFQELLEPALKKGFRQRVTQSWIRSAQKRKLRLRMPSLRVTRQPSFSKDQFEGVVVDYLQPPVEDVALKDFPVPSKDSYLQSETQALIQQERSVPGFSPTPSSLDPGGPPLPHRRSQESVRSLRRASSLHDIDGMRDQWNDLKPNNVNSTSDSDLMRHRAIGRIPQVTLTFGSDRLRPPSPTEIEIIAPSKIKDRTQNVSEKVTQVTQVLSLGADVLPEYKLQAPRIHKWTILHYSPFKAVWDWVILLLVLYTAVFTPYSAAFLLNEQEEESRRTCGYTCNPLNVVDLVVDVMFIIDIIINFRTTYVNHNDEVVSHPARIAQHYFKGWFLIDIVAAIPFDLLIFRSGSDEPQTTTLIGLLKTARLLRLVRVARKLDRYSEYGAAVLFLLMCTFALIAHWLACIWYAIGNVERTGSARVGAMKIGWLDNLADQIGKQYNDSDSTSGPSIKDKYVTALYFTFSSLTSVGFGNVSPNTNPEKIFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVKEFIRFHQIPGGLRQRLEEYFQHAWSYTNGIDMNAVLKGFPECLQADICLHLNRNLLQNCKAFRGASKGCLRALAMRFKTTHAPPGDTLVHSGDVLTALYFISRGSIEILREDVVVAILGKNDIFGEPISLYARPGKSNADVRALTYCDLHKILRDDLLEVLDMYPDFSDSFWTNLEITFNLRDADRIMQPTPSEESDCGYRRARRRRHSLRRNRPDGMDREDSYPDQSCASMGNHRGALSHSQWDELCSSASAGSLSSEDEVKPPGPGRGDMYPPEVRDYPPTVVSLLPPNNSPAGMGAPLERGAHPYTGSLNVYGYWPERRPSQFSESPRRSSSARICHHHRSVCLEERPSELQARLELLQSQLNRLETRMTADINVILQLLQRQMAPVPPAYSAVSTPAHPLPPSSLYRTGGPVLHSPAPIPPTVKINDSLHSSTKSPDSDNVNHKSRESLSSGIHLTVASDDTMSMSPETETEPVAPRSLSVPPHLQTQTQAKAQAKAHAPGPLCGSLRFPSLPEHLENPSTSQDAAAEIQRHLSDPILPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3J2</accession>
    <name>A0A3B4E3J2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Laminin subunit beta-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LAMB4</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569136.1">
        <property type="nucleotide sequence ID" value="XM_017713647.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000030263"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036045.2">
        <property type="protein sequence ID" value="ENSPNAP00000030263.1"/>
        <property type="gene ID" value="ENSPNAG00000016882.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436909"/>
    <dbReference type="CTD" id="22798"/>
    <dbReference type="GeneTree" id="ENSGT00940000162514"/>
    <dbReference type="OMA" id="RRCSCHP"/>
    <dbReference type="OrthoDB" id="5985440at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22301">
        <property type="entry name" value="cc_LAMB4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000074">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000084">
        <property type="entry name" value="Laminin subunit alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000209">
        <property type="entry name" value="Laminin subunit alpha 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000065">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000130">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000010">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000135">
        <property type="entry name" value="Laminin subunit beta 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000001">
        <property type="entry name" value="Laminin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000105">
        <property type="entry name" value="laminin subunit gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056558">
        <property type="entry name" value="LAMB1-4_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056860">
        <property type="entry name" value="LAMB4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013015">
        <property type="entry name" value="Laminin_IV_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF279">
        <property type="entry name" value="LAMININ SUBUNIT BETA 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23219">
        <property type="entry name" value="LAMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24999">
        <property type="entry name" value="LAMB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21199">
        <property type="entry name" value="LAMININ_IV_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51116">
        <property type="entry name" value="LAMININ_IVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017199268" description="Laminin subunit beta-4" evidence="15">
        <location>
            <begin position="21"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="18">
        <location>
            <begin position="25"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="266"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="333"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="396"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="448"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type B" evidence="17">
        <location>
            <begin position="616"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="833"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="881"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="927"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1034"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1086"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1141"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1189"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1270"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1471"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1638"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1726"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="482"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="833"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="835"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="854"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="881"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="883"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="902"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="946"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="958"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1058"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1115"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1141"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1143"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1162"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1189"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1191"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1210"/>
            <end position="1219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1808" mass="200728" checksum="899D62734E949174" modified="2018-12-05" version="1">MAREEISTLLLLMLLVPADLQEGCEGGLCYPSLGDLMVGRAAQLSASSTCGLNGPQNYCILGYLEEEQKCFTCDSRSPYNRHNNHYSHQIENVITTFEPERKMKWWQSENGVQQVSIQLDLETVFQFSHLVLTFKSFRPAAMLVERSKDFGRTWKVFRYFAADCANDFPRVSEGLAESIDDLICDSRYSRPEPSTDGEVVLKALDPNFHIDDPYDPIIQDLITMTNLRVNFTRLFTLGDNLLGRRRRNPQDKYYYALYEMVVRGSCFCNGHASQCMPVYSTRGDTFSEPGMVHGRCMCQHNTMGNNCEKCQDFYNDAPWRPAGNNDPYVCKKCNCNGHSEQCHFDMNHYIATGQVSGGVCDNCRNNRVGAQCEHCQPRYYRDPQRSVQDPAACVPCACHPAGSVEGGLCDPVTGRCMCKQNVEGERCDQCKLGFYGLSQEDQSGCQRCRCNFLGTKRTSPCDQTTGQCICEHFAHGPECDQCVPGYWGLGNTVYGCLPCDCDIGGAFSTECSSVNGQCQCRANMIGLSCSEPAPGYFLAPLDFYLYEAENAAPLDTKCSSYLVRPTPSAITGPPVQKPSPPPYQPLEHTPAPSTLPRCDHYFRSRGYDFMFSDGKFVVVKHERHQARKRRQGQRNISLLPGSSHQIILKPYTPDVPVTWTGPGFVRLQDGAGLRFAITNVPLNLYYHLVIRYATEFTDDWTATVKIVPIGSYDENCPNNPSEKTMVLPSTRRAATLDPPVCLSSGVRYYVDITFEKRSNPDPYCGSRILIDSMGLIPKVESLPNFCTEEALAQYQQYQCVELISRAGEHTLPEVCEKLVASMSAYIHNGAIPCKCNRDGSFSSSCSKFGGQCDCKPNVIGRCCDTCAPLTYGFGPNGCSPCNCEPSGSKAEVCDYRTGQCPCHNKVTGRRCDQCLPGNYGFPNCRRCQCNGLAELCDPITGVCLNCKEHSTGPHCERCVEGYFGDPVSRDTCMPCQCPDVKGSGRFFARTCNKDPNSLVPSCECETGHQGLRCDVCVPGYYGNLLLPGARCKECPCNNNIDPRDGKACDAVTGECLRCLHNTHGPNCQTCRPGYYGNALLRDCRECVCDQLGTELTRCPLDGPCFCDAVTGQCPCRAGVVGVRCDQCEDGYWGLQSGCQPCNCNPQHALSNLCDKITGQCPCEQGYGGKQCGECAENHFGNPDIQCISCDCNMEGTVHPGCDPYTGECMCRPGVSGIFCDECAPGHDPVFPACEPCHTCFLLWEKKVTDITQATTAMLSLIPRPGTVPGDTRLHKRLQELNEKLKNLTSMLGHDQNELDTIEQLLKQIRNTTDTIDPNIIIIDPSQVLNTEIDNIRYEFKRLLDNLERKIKEGPKLDLKALNDTLKKIRKLHADFMKDEEKVKEANGIVEASRQTRQDTKRELAKCQQRPLDVLEKKVKALNVAQLNEEICGAPGDVECEKAKCGGALCGKCGGPSCTGSLPVSENTLSTAEKTEKGLLELLSNLTEAETKIKEVKNINEGIKDKAQDMMNKIINSKNTFEKDKNNIKNLIIRVREYLTADTMEPEDIDTLATAVLDIQLPSSPDEIKKMILDIQNILSNFTHFKEDLRPLQYQAGLAKEMKQKADEILNSTKQIDISEIKKNLNDTAQLQGKIMQGLNEAEDNKDVASKKLKQTTAKLNNVEDNLNSAPTKKLLNDIEALKNKTAMNQAQAAEAKAAADAALDSAADASEGLMEVIEQFKNLTEKNRTQGANEVANERLNNIKTEAENMAKDIEDKLNEIEVLEKRIQDSAQNKERKMKELEELRLKAEGLNDYIAEKVEHYFLCTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3P1</accession>
    <name>A0A3B4E3P1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sphinganine + octadecanoyl-CoA = N-(octadecanoyl)-sphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:67033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36548"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3P1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030313"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018246.2">
        <property type="protein sequence ID" value="ENSPNAP00000030313.2"/>
        <property type="gene ID" value="ENSPNAG00000016987.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234515"/>
    <dbReference type="OMA" id="NINSRMP"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000020">
        <property type="entry name" value="Ceramide synthase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF6">
        <property type="entry name" value="CERAMIDE SYNTHASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="15">
        <location>
            <begin position="137"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030313.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="405" mass="47527" checksum="5B2D5A7D4A44EBBF" modified="2024-11-27" version="2">MDILTFGLLNQWLWQKNFWLPPGATWQDVGNDGIEGSRRPFPRDLLLTLPLALGFIAVRFVFERVVALPLSRTLGVCDRVRARAPPEPKLEAFYLQRKNHYSQSELLGLEKQCGLTVRQIHHWLHLRRNQDKPSNTKKFCEASWRFVFYLIAFVAGLISLIDTPWFWNHRECWRGHPRQAVTEAQYWYYVLELSFYWSLVFCVSVDVKRKDFKEQIIHHIATIFLISFSYCGNYIRVGTLVMFVHDSSDFLLESAKMFNYAGWRKTCDGLFVLFAVVFLVTRLVIFPSRVVYSTIVESLDFFDPFPGYYFFNALLLVLQALHVFWAYLILRMVYKFVFLGKVERDERSDEESEGEEEGDGAEEKSCWEQRKGPLNSRLVSLANNCVLNNLTHQQRNINSRMPKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E474</accession>
    <name>A0A3B4E474_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">OXSM</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">May play a role in the biosynthesis of lipoic acid as well as longer chain fatty acids required for optimal mitochondrial function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:41853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41900"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:41901"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17 19">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556971.1">
        <property type="nucleotide sequence ID" value="XM_017701482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E474"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030488"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018578.2">
        <property type="protein sequence ID" value="ENSPNAP00000030488.1"/>
        <property type="gene ID" value="ENSPNAG00000017214.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429620"/>
    <dbReference type="CTD" id="54995"/>
    <dbReference type="GeneTree" id="ENSGT00940000157768"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="OrthoDB" id="5334845at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000015">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000024">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="20">
        <location>
            <begin position="41"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="18">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="461" mass="48454" checksum="7841F0193873B09C" modified="2018-12-05" version="1">MSLTCCQKLKQYGRALSYIRTCMRAITVQRHYSHPSTGKCPRRVVITGIGLVSPLGTGTSLPWQRLIDGQSGVVALDPAEYKTVPCKVAARVPRGDGDGDFKEERFVGRGEISCMSPATVMALGAAQLALEDCGWYPKSPEEQLSTGVAVGMGMVSLEEIARTATAFETRGYSKVSPFFVPRILVNMAAGHISIKHKLKGPNHAVSTACTTGAHAVGDAARFIAHGDAEVMVAGGTEACVCPLSISGFARARALSTSWNQQPKLASRPFHPEREGFVMGEGAAVLVLEEYGHAVERGARVYAEVLGYGLSGDASHITAPTADGDGAFRCMSAALRNAGVSPSDVTYVNAHATSTPLGDAAENAAIKRLFQQHSQSLAVSSTKGATGHLLGAAGALEAAFTALACYHGTLPPTLNLDHTEPEFDLNYVPCVSQPWSTQGRRIALTNSFGFGGTNASLCLASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E6H9</accession>
    <name>A0A3B4E6H9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SPRY-associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PRRG1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E6H9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031283"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020113.2">
        <property type="protein sequence ID" value="ENSPNAP00000031283.2"/>
        <property type="gene ID" value="ENSPNAG00000018546.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286899"/>
    <dbReference type="OMA" id="MIWERKL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SPRY-associated" evidence="5">
        <location>
            <begin position="176"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="45"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031283.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="263" mass="30838" checksum="1434910314287060" modified="2024-11-27" version="2">MPVLLLSRPAGLCCFIQQHFDRRSYPTRCAQSAVEDSERIFTELIRSIEKKRSEVTKLIRAQEEAKLSGAEELLEKLEQEIADLQRRRTELEQLSHTEDHIHFLQSFQSLCVSSGSEDSPSITVHQHLSFDGVRRSLSELKERLEKFYREEFSKIPPHVQILPSEPNNREDFLQYFCQLTLDPKTAHPHLILSEKNRVVTFSNEVQSYSDHPERFDCWDQVLCKESLSGRCYWEVEWSSDGGFVYTQNIIYTVWFMNCVIFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E780</accession>
    <name>A0A3B4E780_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin conjugating enzyme E2 A</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E780"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031104"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019782.2">
        <property type="protein sequence ID" value="ENSPNAP00000031104.1"/>
        <property type="gene ID" value="ENSPNAG00000018260.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155075"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="46"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="197" mass="22190" checksum="5A96611AFB6D1EB0" modified="2018-12-05" version="1">MAVATCCLFNCSHYIRVITALSGAFVYVPVTGWKGNFCAVTSSCVDFLFFFTDQRGLPSKMVRSTFLVQCAASIDSIDKLHCYLYSASVCCPYFAGTFKLTVEFTEEYPNKPPTVRFISKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E829</accession>
    <name>A0A3B4E829_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">YKT6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasmic vesicle membrane</location>
            <topology evidence="10">Lipid-anchor</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E829"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031446"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020440.2">
        <property type="protein sequence ID" value="ENSPNAP00000031446.1"/>
        <property type="gene ID" value="ENSPNAG00000018811.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015164"/>
    <dbReference type="OMA" id="MINEIYM"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15867">
        <property type="entry name" value="R-SNARE_YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000020">
        <property type="entry name" value="synaptobrevin homolog YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045848">
        <property type="entry name" value="R-SNARE_YKT6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Longin" evidence="12">
        <location>
            <begin position="17"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="13">
        <location>
            <begin position="147"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="207" mass="23701" checksum="79344FFEC484D405" modified="2018-12-05" version="1">MSPGVCTATMKLLCLSVLYKGETKANLLKAAYELSSFGFFQKSSIQEFMTFTCGLIVERSAFGTRASVSEKEYMCHMHIRDDGLSAVVIADSEYPQRICFSLLDKVLDEFSRQVDDRQWPTGTPETIRFTVLEEYLKNYQNPREADALTRVQAEVDETKIILHRTMESLLDRGEKLDDLVQKSEELGQTSKAFYKTARKQNRCCKMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8G7</accession>
    <name>A0A3B4E8G7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Isoaspartyl peptidase/L-asparaginase</fullName>
            <ecNumber evidence="5">3.4.19.5</ecNumber>
            <ecNumber evidence="6">3.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Asparaginase-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Beta-aspartyl-peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Isoaspartyl dipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">L-asparagine amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>4,8-dimethylnonanoyl-CoA + (R)-carnitine = O-4,8-dimethylnonanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44860"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77061"/>
            <dbReference type="ChEBI" id="CHEBI:84654"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.5" evidence="5"/>
    <dbReference type="EC" id="3.5.1.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017576123.1">
        <property type="nucleotide sequence ID" value="XM_017720634.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017576124.1">
        <property type="nucleotide sequence ID" value="XM_017720635.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000020623.2">
        <property type="protein sequence ID" value="ENSPNAP00000031554.2"/>
        <property type="gene ID" value="ENSPNAG00000018965.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441219"/>
    <dbReference type="GeneTree" id="ENSGT00740000115744"/>
    <dbReference type="OMA" id="IHVMESA"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016406">
        <property type="term" value="F:carnitine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033345">
        <property type="term" value="P:L-asparagine catabolic process via L-aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04701">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000056">
        <property type="entry name" value="Si:ch211-256m1.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.20">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042572">
        <property type="entry name" value="Carn_acyl_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF42">
        <property type="entry name" value="SI:CH211-256M1.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="15" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HIT" evidence="29">
        <location>
            <begin position="1095"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="28">
        <location>
            <begin position="1263"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="26">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="25">
        <location>
            <position position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00030667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031554.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1580" mass="174135" checksum="6882F1A5F114768D" modified="2024-11-27" version="2">MAQLLDYPIPELDTTIKEAGRVLQLILSPEEYVQYKNALSQQREALQEAQEKLQASASGNENWVTEQFKKRLLSCCDPLPTSTAIPSVLPPSKSKGESAQLERASTLLWAAAKLYSEPWLIEGDVPTERTQQSEVFASSRLPGENQDQLKVYPESLHAIVTCKGGIFPIQILRNPGAEGTVTALPLLDIYTQLAHVYSLPAAEAVQDPSTVCGLSVLHRQTWHAIRKEILKGGGEAAESLEVIESAIMAVSLEDCPAPTNLADILNAVRLGGADGQCLRYYDKVVNMVVFKDSVAGMVFEHSALDGMVAGLVVERMWHLSESQNIEHMKTQTLINGSDVASSHPKALKFPLGGINVPSQPLNSYLQVSNPVITFEVSSFPDVFTTLRGHRGLYDAWINFTLQLSLKQILGETAASYIMVTPTHMRHYKHGRCDPTYSITMQSRKLVTALESCVGPDNVPRYTKELFCQFHLAFLEHKNLIKMTKNGHGVGPHLAVLRRSMSQDNPLKKYLDLFGCPSIYLTGSDLMEGVECAVGNVYATNQLAVTYLGKKDKVRLVLNGKGTFSSILGKLAMCLELNLKLVMLLAVRYAIAGQMGAIECLLQKEEQSINSTTVDTKVVDPKGGKKVKQKGCLDSSAAEKSSFTLVIHGGAGVEMILSHKVVEVIEFALETALTLGAQVLSHGGSSLDAVQRSVSALEDCFLFNAGKGSVYNRSGEHEMEATIVDGHEKNSGSVACVRCVKNPVKAARHVMEKSVHSLLTGDGAEEFLETLQEREKPVTPEYFHTDVRYKELAIKLNSSKNNHPQTVGAVALDCWGKLAAATSTGGLVGKWKGRVGDTAIVGAGIYADKKLAVTCSGDGDAFLRETVAHKVASLYNLKGYTLAQACREVIYEDLGANCAGIIAVDHKGEAAVETNAGVMFVASMVDGVARAEVYRPVMSFANVIWETDELIAHLHPEPWTPGTTILTRKALNGPNSIFQLPMPDYMAMLLGAQTVANLLCEKLGVYRCALVSMPKPDKPAHIKILPIHGLEPNWKPHLAEEEQFHIHDPGYCSSKSGPRCEDAYLDQVQSKIRACLPTPNAPSSYDFYGDPSNDGLFSRIVRGEEQQWRVWEDTEHVAFLTPFPNTPGLTVLVPRRPLTSDIFRLDRSDYTALILATQKMAQLLQEGMGAHGVALIFEGFEIDYAHAKLIPLVTKPGDMLPAVPLEFCPTYPGYVTSADGPPASAEALKDIHTKITRTTPPRTWEQPQSHSTLAIKSQWYRNLFQIQNTLFHSTVEYFSNKCRYAYALTPITTDTISSPMGLGSDSEPVFVNMLGQDVYLADSMQFVLEYFLRFQEGLPGTYYLSPSFRGEDPDSTHLNQFYHIECELLGDMDEAINIAESFVAHLTQAMLRNHSKIIESSAGTLSHVQAMLKQLEEPLPRVTLDQAITVLPSADCLEWVQEGQPQFGRKLTRKGERVLIEKYGGAVWLTEMDHLGVPFYQAYVEGSGRKKAKAADLLLGLGETVGLGERHSTPEMVQEALRHHAVPEESYKWYMNMRQIIPLSTSGWGMGTERYLCWLLQHNDVRDMQIIPRLKAKKYMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E9T9</accession>
    <name>A0A3B4E9T9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Steroid receptor RNA activator 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Steroid receptor RNA activator protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SRA1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">SRA1 RNA exists in a ribonucleoprotein complex containing NCOA1. The RNA also forms a complex with PUS1 and RARG in the nucleus. Interacts with AR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the SRA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571737.1">
        <property type="nucleotide sequence ID" value="XM_017716248.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E9T9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032458"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022505.2">
        <property type="protein sequence ID" value="ENSPNAP00000032458.1"/>
        <property type="gene ID" value="ENSPNAG00000020396.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438456"/>
    <dbReference type="CTD" id="10011"/>
    <dbReference type="GeneTree" id="ENSGT00390000001803"/>
    <dbReference type="OMA" id="VPGIQHD"/>
    <dbReference type="OrthoDB" id="5982138at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.940.10:FF:000006">
        <property type="entry name" value="steroid receptor RNA activator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009917">
        <property type="entry name" value="SRA1/Sec31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040243">
        <property type="entry name" value="Steroid_recept_RNA_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18834">
        <property type="entry name" value="STEROID RECEPTOR RNA ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18834:SF2">
        <property type="entry name" value="STEROID RECEPTOR RNA ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07304">
        <property type="entry name" value="SRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SRA1/Sec31" evidence="18">
        <location>
            <begin position="70"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="234"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="236"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="284" mass="31300" checksum="B939DE27564048A8" modified="2018-12-05" version="1">MDNFYIKPGNQERGWNDPPQFSYGLQTNPGGTKRNILNKRVPPPQLTGQSPGPGLPPSSAASSVPPSNPGPPPVGGLTPPPPKSRISSGGLSTGSPLSTSKTQTNSSVPAENEPELDEVQALLLWSLAACRQSVKKQVCDDVERRLKLFEEMWKSGKLSLPVKCRMKGLSQELKNKKWDAADEIHRALMVDYVNEVSQWMVGVKRLIAETRNLSPELFQTEDTILMQVEKDLPQTEDVQPQTEDVQPQTEDVQPQTEDVQPQTEDVQPQTEETAQSDISTDPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EA52</accession>
    <name>A0A3B4EA52_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-O-(1Z-octadecenyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H2O = 1-O-(1Z-octadecenyl)-sn-glycero-3-phosphate + N-hexadecanoylethanolamine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71464"/>
            <dbReference type="ChEBI" id="CHEBI:137009"/>
            <dbReference type="ChEBI" id="CHEBI:137017"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:53185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41143"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:77580"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:41144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N,1-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(9Z-octadecenoyl) ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71466"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85222"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:56461"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45544"/>
            <dbReference type="ChEBI" id="CHEBI:2700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85223"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:45545"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoylethanolamine + 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53168"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71464"/>
            <dbReference type="ChEBI" id="CHEBI:74544"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:53169"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537892.1">
        <property type="nucleotide sequence ID" value="XM_017682403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EA52"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033427"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024320.2">
        <property type="protein sequence ID" value="ENSPNAP00000033427.1"/>
        <property type="gene ID" value="ENSPNAG00000022079.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411042"/>
    <dbReference type="CTD" id="406482"/>
    <dbReference type="GeneTree" id="ENSGT00940000160759"/>
    <dbReference type="OMA" id="KWAIMRK"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08612">
        <property type="entry name" value="GDPD_GDE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF3">
        <property type="entry name" value="LYSOPHOSPHOLIPASE D GDPD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="14">
        <location>
            <begin position="39"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="316" mass="36563" checksum="DE4AD42A591F3FB9" modified="2018-12-05" version="1">MSSFMYYLLPAVGGYTLASLYLLKNPHILHKKKQPAFYCTHISHRGGSGERIESTMEAFTHAVEVGTEMLELDCHMTQDGHLIVSHDDNLLRQTGHDVSISSINFEELPLYKERLEVTFYAGRYSTGSDRKFALLEDVFRTFPKIPVNIEVKEKNRELIEKVSALVKKYDREDITVWASVKSDIMKECRKVNSSMPYMFTETRGLQLLLLYYTGLLPFVPLGESFLQYYLPQVINRTYIPDSAILRNRMVISLIEKLTLRKGLFQHLKDRGIQIHLFVCNEEQDIEAAFEAGATGVMTDYPYLLSNYLRTHKQRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EB14</accession>
    <name>A0A3B4EB14_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mif2/CENP-C cupin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BBX</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EB14"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032421"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022337.2">
        <property type="protein sequence ID" value="ENSPNAP00000032421.2"/>
        <property type="gene ID" value="ENSPNAG00000020338.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282920"/>
    <dbReference type="OMA" id="HSATTIF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025974">
        <property type="entry name" value="Mif2/CENP-C_cupin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11699">
        <property type="entry name" value="CENP-C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mif2/CENP-C cupin" evidence="2">
        <location>
            <begin position="1049"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="630"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="888"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="750"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="790"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="893"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032421.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1133" mass="125412" checksum="60F3D861CA2F05A4" modified="2024-11-27" version="2">MDRKMMHLGRAKKQLRYFQTDEKTSCSLKIKDVFSLNDLDNIFDDLDSGSDSAMPQLSLLPKSDKIVEHGKEDSSPDLKISSPFGAEEPEALAPENPIQEDHLNGKESPFKEIAPIKTSSPIDLLPAEDGAGDIERPETSPILFGVEDEEPVRHDFSKSLPNQRSESREEFLKGGNESLVSPPTRLEFGKPATQDVVSVTRCMPAQSSNTTSQECQTSAPEAREESQIDGSPSLLFPVSAQNSHSEPLLKEQSKNQVPEVKLDVEVKESSFQQKLRNAFKPKTLWKQEARKPQARALSPLELEDDFMILEDDAPILFTIPKKANSSQKEKHALECVSAKPDDKKSPSQAESAAPKLEDEPGTAKTKKTKGKHGNTYSKGDKNTMTQATVEERNEPLMEVEEVVGDCEPSSVPEVNQDADLPDTGKWRRNNKAHIKLGKKNGKNYDQAIIPVEEPASKRKKPAKITGDTNNAQEEHASNAKKKPNKAKSIVKGGIPEGSAKLDKQINDDAALTDKQVPKQQEQEDKLHKTNEPLLQPTEQPDVQSTAPIKKADKRPASKRGTSKMVKKVQKTKECIKVKEIPVPESDVSAEGSVTAKRKRKPPGEWWLTTGKQNEGNTQVQEVAVQESLQVQKSNVKRKRKETRAISAQSTEEQESHSKTIKHVTLQKVGEKVKKSNNSTSDQKNPKVAGATQKTKSAASTQRQIQSKTPAPSVGEAVHEDVEQVSSKACGPPRRQTLSSGEKRVFEKLYTTDSQYGSTQKHPPSALHQPESLPVKRQRKPPCNWWEVPQSRGSVESSLSPPSSSPRKSKAQTALPCNVFDKVANVVSSQKTVVRAQKKNKINTIHTPKSAKSSLATFKAILASGKPESSTMRGFERRHKGRRNLLHSLEDQSEQSSENILSDQQHGSSHATFDVHPSGAIMDSFKARKTADARLSDGSNRASSGIGFKSGPSSMIELERFEETEDSDLPSSRIVPCIRHVPRVLLDCDLCGPPLRPIVLEREDWDNLCIWFAHLWPSSSKEGKVFSVISPDDFHWYSHGGRAMGHMVDLQNSTFSNGKILLGSYMKKPLQVDLHAVTVFNVASSCVRVEIDGVKMVYNSGETFMTPCGKFYSIHNICREPAVLWYHRMLPNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EEV5</accession>
    <name>A0A3B4EEV5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CASZ1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="42514.ENSPNAP00000033756"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038122.2">
        <property type="protein sequence ID" value="ENSPNAP00000033756.1"/>
        <property type="gene ID" value="ENSPNAG00000003723.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008187"/>
    <dbReference type="OMA" id="GRACPHN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040373">
        <property type="entry name" value="CASZ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451">
        <property type="entry name" value="TRANSCRIPTION FACTOR CASTOR PROTEIN MING -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN CASTOR HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="634"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="751"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="1682"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="817"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="914"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1000"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1464"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1814"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1930"/>
            <end position="2032"/>
        </location>
    </feature>
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        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="839"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="914"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1015"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1486"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1513"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1526"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1958"/>
            <end position="2001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2010"/>
            <end position="2021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2032" mass="223159" checksum="E7FA20A24CA4986A" modified="2018-12-05" version="1">MPCFVERIPSERQGEVREREEGQLLPAESGLCTEKTSGKSTMAAKRKGGLKLNAICAKLSRQVVYDGSSQNAEGDPSLADNSERGSSHYEEGDRPDSDYSEGLSLGQSLEDDQKRREAIEKWVNGEYADDPASIDEDEPRGPKASSGDEGPPEGVYMVQPKGCSDEEDNGDEAETIPVSHEDSYHDDRDSADSPHKETSSSETPNCQASFSSPGEASALRDYAASTMNEFLHMFGYDDQQVRDELAKKISFEKLKAATSDPSSLSSEEATRRARFSKYEEYIRKLKAGETLPWPIHSKPEELNSKMTQEKTSTLIQPSSCLPAAEGQIFSAGMEHKQAQLSASAGNSSHNLASRASKYDFFIQKLKMGESLRQQNGNAYKRPSKYDLENVKYLHLFKPGEGNPDMGGAIAFKTGKVGRPSKYDIHNIQKLIPSKSDPSIMPGVLSSTPATPGTPMIPTAASAGAVGPPCLPVDQVGHLTFSPEYMKSSFSKTDSITTGTVSSVKNGLPPEKPVTEDINVYQKYIARGPLRSSQKHLKPSPPHESFALPPTAKRFSGSQHCGHVHCAYQYREHYHCMDPECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCNKVYTSTSDVMTHENFHKKNAQLMNDGFQRFRATEDCGTVSCQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRQGCGFTFTSTSQMTSHKRKHERRHIRSSGVLGLTSSFLLPKEEQEESSNDDLVDFSAISSKNSSLSASPTTQQQPSSTLPHLMSTPTTAVASSSHNVKPTTALPPASHMSTLLSQALPSNMPVALAMASSALGGASNPFFPLLPRLPLQLPPNAASMISAASSGVHSTPRDSQAPSSSSAGELPPASTPSSYAASSIMEKISASKGLISPMMARLAAAALNPASNPETGNGQQAQAGRFNPVQVKQEPMDGTSGASQESMQEHSLDLSKKDPSNESNGHPAPANTSLLSSLMNKMTQVNPGFFSALTMKSELEGGQAPENSETVQYLSRLMKRPAPEKPAELWKTYLRRYDTDEVCDGQCDFLHKVHFHCLVEDCGALFSTVDGAIKHANFHFRANLKVKPEPSFNEGKDSNEGAPNQLTAPISMASSPPMEVPSVAASGGYGSSPSLLAWKQLTGSIPQLPATMPNMPATSPLATTSLENAKPQVKPGFLQFQDNDPCLATDCKYSNKFHFHCLFGNCKYVCKTSGKAESHCLDHINPNNSLVNVRDQFSYYSLQCLCPNQHCEFRMRGHYHCLRPGCFFVTNITTKLPWHIKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIREGCMFSFLLKHQMTSHARKHMRRMLGKNFDRVPSQVVPHGHQSEEMQHMASLASSGTIASHPSLTTSFSNMMEENDDYMDYTGGGSPLGLSSESSNQDRSCTSTPVGNDSSPAGPGCQLPPPASTSSAETPPTSQSAPPPPPPPQPQAPLQSQPPSFPPTLLRPPLPSLPYLLSPSCVSYSLLSASLGATRSVVMPTNTPAFSPIIATPSPVKNDVPIVQDAAGNTISIPTATGAKKRFWIIEDMSPFGKRRKTASSRKMLDEGMMLEGFRRYDLYEDCKDTACQFSLKVTHYHCTRENCGYKFCGRTHMYKHAQHHDRVDNLVLDDFKRFKSSLSCGFADCQFSGNSTHFHCLRCGFRCTDSTKVTAHRKHHGKQDVISAAGFCQFSSSADCEVADCKYKLKCSHFHCTYPECKHTVVGMSQMDSHKRKHEKQERGEPPSVSPNQEGHHQMGLVPPPSSLNLPPTSPGVAQGLPHNSGAAIYLPPGMGEYPEHHRPMVNLDSSLNLGTDTSSSLFFLKNAAGLGLADSLDLSKKLQRDSLTTAPGATPNAPIMSLGHSRDDTTRTSGEPEDELSPEEEPTAHEEEEDEEDEEEEPEEDLNTDSYDDSMPEPDGEKDNSESFDASMNHADTSQLDKEEPEADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFH7</accession>
    <name>A0A3B4EFH7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="12">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PPEF2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFH7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033966"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025405.2">
        <property type="protein sequence ID" value="ENSPNAP00000033966.2"/>
        <property type="gene ID" value="ENSPNAG00000023005.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160831"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000341">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF527">
        <property type="entry name" value="PROTO-ONCOGENE TYROSINE-PROTEIN KINASE ROS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="103"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033966.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="513" mass="58523" checksum="0403C361D0CD84EF" modified="2024-11-27" version="2">NIWHFIRATNTFISKINCKLSDYNELFHNLYEIFKISYQLHVFYRRRQNKAPETNSQTSLYSQDRELDDIRGLVGLGNACYAVSLLPMHKELESLPVFPRECLKLQKLLGSGAFGEVYEGVTVRDPHSDTVPEQRVAVKTLRNGASSQEKIEFLKEAHLMSQFSHPNILRLLGVCVRNEPHYIILELMEGGDLRSYLRGARPTNNHGKLLNLTGLLDISVDVANGCVYLEKLHFVHRDLAARNCLVSVRGYADPDRVIKIGDFGLARDVYKSDYYRKRGEGLLPVRWMPPESLTDGIFNKHSDVWAFGVLLWEIMTLGKQPYPAFSNQEVLHHVNTRGRLPAPAECPQSLYKVMLECWSKEPRERPSFRSLHMSLVKLRETETLSEEGKTGHVNEAYTEDGEIPLTLQNETKPKANVKTSIFSNSLQVHYLALRSYQIFWKLRRHCCNFNSEIFSRSCLMQDFSCTTVSGSLLLYVISSDWSGLQAGQFSTQILLIWSGAVIIHAECGLMLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGF1</accession>
    <name>A0A3B4EGF1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557682.1">
        <property type="nucleotide sequence ID" value="XM_017702193.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGF1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025996.2">
        <property type="protein sequence ID" value="ENSPNAP00000034296.1"/>
        <property type="gene ID" value="ENSPNAG00000023484.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430042"/>
    <dbReference type="GeneTree" id="ENSGT00940000163148"/>
    <dbReference type="OMA" id="MGMGPTD"/>
    <dbReference type="OrthoDB" id="8407241at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF98">
        <property type="entry name" value="CRYGM5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="41"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="91"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="132"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="178" mass="21316" checksum="2A2C9D04C63BA297" modified="2018-12-05" version="1">MGRIIFYEDKNFQGRSYETSSDCAELTSYLSRCNSCRVESGCFMVYERPNFMGHQMLARRGEYPDNQRLMGMSMSDCIRSCRMIPMHRGPFRMRIYERENFGGQMHEVSDDCDSFVDRYHMSDIQSCHVMDGHWLMYEQPHYRGRMVYLRPGEYRSYRDMGMSSMLRFSSMRRIMDTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHB1</accession>
    <name>A0A3B4EHB1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559489.1">
        <property type="nucleotide sequence ID" value="XM_017704000.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559491.1">
        <property type="nucleotide sequence ID" value="XM_017704002.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559492.1">
        <property type="nucleotide sequence ID" value="XM_017704003.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559493.1">
        <property type="nucleotide sequence ID" value="XM_017704004.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037389881.1">
        <property type="nucleotide sequence ID" value="XM_037533984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHB1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035882"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029511.2">
        <property type="protein sequence ID" value="ENSPNAP00000035882.1"/>
        <property type="gene ID" value="ENSPNAG00000006005.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431077"/>
    <dbReference type="CTD" id="559050"/>
    <dbReference type="GeneTree" id="ENSGT00940000164496"/>
    <dbReference type="OMA" id="TGPCDIS"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="88"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="10">
        <location>
            <begin position="346"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="45870" checksum="D772D25BE2CCDB43" modified="2018-12-05" version="1">MVKKTKNPVTYTKMKGIVSYFAALIKERKFGSADLLQKLGTRHPIYRHMDAECFLRPEGSSVCDEAGAAALCFTRTTQELQRASAADFDYLKVIGTGSFGKVLLARHRENDRYYAVKVLKKQMILKKNVEGHVMCERSVLLKTVNHPFLVRLHFSFQTRDRLYLVLDYADGGELFYHLQKERVFTESRARFYAAEMASALGYLHSLHIVYRDLKPENILLDSAGHVVLTDFGLCKEGIVGRATTRTFCGTPEYLAPEVLQQREYDRTVDWWGLGAVLHEMLYGLPPFYSADRMVMLSNIMFQPLVLKAGVSKAGRDLLNRLLNRDRAKRLGAKHDVAELQHHSFFSSIRWDDLVAKKIKPPFVPSLSGPDDLTNIDPAFTNLPVPQSLGVCEDAGMTFPGFSYMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHV0</accession>
    <name>A0A3B4EHV0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tripartite motif-containing protein 16-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017542043.1">
        <property type="nucleotide sequence ID" value="XM_017686554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHV0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039133.2">
        <property type="protein sequence ID" value="ENSPNAP00000035323.2"/>
        <property type="gene ID" value="ENSPNAG00000025263.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413856"/>
    <dbReference type="CTD" id="561829"/>
    <dbReference type="GeneTree" id="ENSGT01150000286950"/>
    <dbReference type="OMA" id="GNKPKWT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="144"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="356"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="199"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035323.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="61886" checksum="BA24FBD192ABFD78" modified="2024-11-27" version="2">MAQVEQLLGREQFTCPICLDLLRDPVAIPCGHSYCMSCIDVYWTNNDTRKAVSCPQCRQSFNPKPILNRNTILAEMVEKLKVAVQPSVRSVASEVECDLCTETGGKAVKSCLVCLVSYCEAHLQPHYRSPALKKHQLVEASRHLQEKICPQHNKLLEIYCQTDQRCICLLCVIDGHKGHDTSSAASEGAKQQKQLGLTRRKFRLRIHAKEKELMELRQSVETLKISVHSSAEGSDQIFGELVQSVQRRCQEVRELIQDQERMAVRLLEKLEQEILELKERDHKLELLLHTDDHIHLLQTYRLFSAPPGPPDAHSISIAPFSDLSNVTSAITALRTRLEGVFKAEWPKIVRAASTVKFLQSPLPKIRPEFLYYSRQLTLDPRSAHRELSVSPDCRTASVRAREHSCPDQPERFDYWCQVLGSQGLQGCCYWEAEWSGMGITVAVACKDLARKGDGSDAHFGRNANSWSLFCSRKAYAFWHNNTVTRIPGPSSSRIGVYLDHNAGTLAFYSVSENMRLLHKVHTTCTQPLYPGFEFTCYGALVTLCQME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHX5</accession>
    <name>A0A3B4EHX5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ribonuclease P protein subunit p29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">POP4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a catalytic RNA moiety and 10 different protein chains; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
            <location evidence="2 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541312.1">
        <property type="nucleotide sequence ID" value="XM_017685823.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHX5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035348"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028228.2">
        <property type="protein sequence ID" value="ENSPNAP00000035348.1"/>
        <property type="gene ID" value="ENSPNAG00000005591.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413348"/>
    <dbReference type="CTD" id="10775"/>
    <dbReference type="GeneTree" id="ENSGT00390000010067"/>
    <dbReference type="OMA" id="IPKSECV"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.210:FF:000001">
        <property type="entry name" value="Ribonuclease P protein subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027081">
        <property type="entry name" value="RNase_P/MRP_p29_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR027081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="220" mass="25337" checksum="D8E80BB84405E870" modified="2018-12-05" version="1">MERVVLSRIPPDEKKLVGVRARHGKAAQQFTQAFLKSSIPRMSSDAIEDILLHRAVVLRYTRVKKTRGKKTKAKGLNAKERRELKVFQLKPEHQKYELFLPLHELWKQYVADLCNGLKPESNPQMIQQKLLKADFHGAVLTVVRSKCPSYVGTTGILVQELKHVFKIITKEDRLKTIPKRNSVFSVEMGDFITHIYGSRFELRSSERSAKKFKVKGNIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EI89</accession>
    <name>A0A3B4EI89_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EI89"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028916.2">
        <property type="protein sequence ID" value="ENSPNAP00000035585.2"/>
        <property type="gene ID" value="ENSPNAG00000025714.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161369"/>
    <dbReference type="OMA" id="GGTMEMF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF359">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="38"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035585.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="357" mass="40809" checksum="0F5769B4185E6BDA" modified="2024-11-27" version="2">MLLNLSITEFVFKGFDSVQNPLMVGFVLLILYIFVVITNTANIYFIVMDKHLHQPMYLFICNLAFVDIVLCTSTCPTMIGTLIVGYKSMSFAPCMVQMFVFHLCSLMEGFAISIMAFDRFIAISSPLRYHSILTNVRCVLISIVLWLAGSAIMAMLPATVLPLQICYSVVKHMFCDYASVIKATCVDPEPYFNMVSILTSVLIFGTFSFICLSYVKIVIVVVRMSSQSDKKKAFNTCFSHLIVIICFYAPTFTRIVLTRFGLVLTVEERNGLMLGSVLGPAVINPFIYCFRTKEIRNKLFKIILKYIRNAEFLFFHRSLFAHTHQQQFAMKVRYVSSLNFLDHVYFITLLKCLSHYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EM49</accession>
    <name>A0A3B4EM49_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cystatin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Stefin-B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CSTA</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EM49"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037552"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034007.2">
        <property type="protein sequence ID" value="ENSPNAP00000037552.2"/>
        <property type="gene ID" value="ENSPNAG00000004571.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155717"/>
    <dbReference type="OMA" id="LPHENQP"/>
    <dbReference type="OrthoDB" id="2429551at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071220">
        <property type="term" value="P:cellular response to bacterial lipoprotein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000001">
        <property type="entry name" value="Cystatin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF21">
        <property type="entry name" value="CYSTATIN 14A, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="9">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037552.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="100" mass="11024" checksum="1B0F4F26D9BFB33E" modified="2024-11-27" version="2">MDQVVGGTGSVQVATPEIQKICDEVKPQVEQKTGKKFRVFVAKSFTTQVVAGTNYFIKVQVGGEEYAHLKVFQSLPHAGHKLELLSVQTGKTLDEPIVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IPT2</accession>
    <name>A0AAR2IPT2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NAGPA</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IPT2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084315.1">
        <property type="protein sequence ID" value="ENSPNAP00000041715.1"/>
        <property type="gene ID" value="ENSPNAG00000007669.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163299"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000013">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF22">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043636028" description="SRCR domain-containing protein" evidence="12">
        <location>
            <begin position="26"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="28"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="133"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="237"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="333"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="435"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="536"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="401"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="643" mass="69775" checksum="672DA63BCF4388D4" modified="2024-11-27" version="1">MMELQRPAALLTAVILMVVCSTAEMAGVRLVDGDGHCSGMPQVLYEGNWSIVDSVLFDYRNAMVVCRELNCGNAVNVTFRENVRKEDINDTFLSITCEGKESAFSQCKQRDQDPLDRINNIIHSTDAICSDSVRLVDGAGLCSGRLEVKSHQSWTSVCETGFDQQDAEVVCRELGCGTPLTLQGALFGEGKHPFGTKDFQCKGTENRLLTCSTSEREEHTCALGKAVGLTCSEPDDVKLVGGSSRCAGTVKVFHTGEWRSVTAVQWSIKEAAVVCRQLDCGSAVVASRRSSSGPSPWSVQIECEGSESALRECRSYYNQHDWYRAEVICSDSVRLVDGAGLCSGRVEVKPHQSWTSVCETGFDQQDAEVVCRELGCGSPLTLQGALFGEGKHPFGTKEFQCRGTEDHLLKCSTSEREEHTCALGKAVGLTCSDSVRLVDGDGLCSGRLEVKTHQSWTSVCETGFDQQDAEVVCRELGCGTPLTLQGALFGEGKHPFGTKDFQCNGTENRLLNCSTSDREEQNCTRGKAVGLTCQYVKLVDGSSRCAGTVMLYDKKWRTVEGNAWSMKDAAVVCRQLDCGSAVAVTSQYLEMATYEDNVQIFCSGSESGLKECAFYDVPWSLSSAGVICSGQQEATTAAVQKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IQ46</accession>
    <name>A0AAR2IQ46_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Bone morphogenetic protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BMP4</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546411.1">
        <property type="nucleotide sequence ID" value="XM_017690922.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017546412.1">
        <property type="nucleotide sequence ID" value="XM_017690923.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017546413.1">
        <property type="nucleotide sequence ID" value="XM_017690924.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017546414.1">
        <property type="nucleotide sequence ID" value="XM_017690925.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IQ46"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087453.1">
        <property type="protein sequence ID" value="ENSPNAP00000041825.1"/>
        <property type="gene ID" value="ENSPNAG00000031817.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108423540"/>
    <dbReference type="CTD" id="652"/>
    <dbReference type="GeneTree" id="ENSGT00940000159502"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060562">
        <property type="term" value="P:epithelial tube morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000103">
        <property type="entry name" value="Bone morphogenetic protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000005">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF165">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043826407" description="Bone morphogenetic protein 4" evidence="17">
        <location>
            <begin position="20"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="18">
        <location>
            <begin position="284"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="400" mass="45679" checksum="C0DE778595AABCC9" modified="2024-11-27" version="1">MIPGNRMLMVILLCQVVLGESNHASLIPEEGKKKVSGHHPGQSHELLRDFEATLLHMFGLQRRPRPSRSAVVPQYLLDLYRLQSGEADEAGAQEVSFEYPERSTSRANTVRAFHHEEHMEQVQSDGPYTTGTPLRFLFNLSSIPEDELLSTAELRLYRKQIEEASSEDAGNEGLHRINVYEVLKPPRPGQLITRLLDTRLVHHNTSNWESFDVSPAVLRWTRDRLPNHGLAVEVLHLNRTLRHQDKHVRISRSLHQAPGEDWDQLRPLLVTFGHDGKGHPLTRRTKRSPKQRGRKRNRNCRRHALYVDFSEVGWNDWIVAPPGYQAYYCHGECPFPLADHLNSTNHAIVQTLVNSVNSNIPKACCVPTELSAISMLYLDETDRVVLKNYQEMVVEGCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IR64</accession>
    <name>A0AAR2IR64_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">KH domain-containing, RNA-binding, signal transduction-associated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Src-associated in mitosis 68 kDa protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the KHDRBS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564149.1">
        <property type="nucleotide sequence ID" value="XM_017708660.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IR64"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081267.1">
        <property type="protein sequence ID" value="ENSPNAP00000042200.1"/>
        <property type="gene ID" value="ENSPNAG00000032759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433834"/>
    <dbReference type="CTD" id="406516"/>
    <dbReference type="GeneTree" id="ENSGT00940000155718"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000036">
        <property type="entry name" value="KH RNA binding domain containing, signal transduction associated 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032571">
        <property type="entry name" value="Qua1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032335">
        <property type="entry name" value="Sam68-YY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF30">
        <property type="entry name" value="KH DOMAIN-CONTAINING, RNA-BINDING, SIGNAL TRANSDUCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16274">
        <property type="entry name" value="Qua1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16568">
        <property type="entry name" value="Sam68-YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="K Homology" evidence="21">
        <location>
            <begin position="59"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="184"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="322"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="354" mass="39273" checksum="1902F600C428A06D" modified="2024-11-27" version="1">MENEGSKYLPQLLAERDSLDSSFIHAMKLISAEIDRVQKEEAPEKDTETYLDLFTAKNIKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEGLRRSGEPKYAHLSMELHVFIEVFAPAPEAYLRMAHAMEEIKKFLFPDMMDEICQEQFMEMGYLNGNQDHGVRGRGGPLARGRGAPPAAPRGRGMPPMRGGVPRGGPGRGGGVRGAPAGRGGPPTQPARGAAAGRARAPAPGPQRMPPPPPRPPAQESYEEYSYDESYAEPAYESYDSYYSQPQTEPEYYDYGHGETQEGYENYAQDDWNGTQRAPTGGKVSAQRQTKGAYREHPYGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ISB9</accession>
    <name>A0AAR2ISB9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2</fullName>
            <ecNumber evidence="3">2.7.1.105</ecNumber>
            <ecNumber evidence="4">3.1.3.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">6PF-2-K/Fru-2,6-P2ase heart-type isozyme</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Synthesis and degradation of fructose 2,6-bisphosphate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Forms a heterodimer with PFKFB3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.105" evidence="3"/>
    <dbReference type="EC" id="3.1.3.46" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2ISB9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047949.1">
        <property type="protein sequence ID" value="ENSPNAP00000040922.1"/>
        <property type="gene ID" value="ENSPNAG00000012439.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182835"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000001">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000047">
        <property type="entry name" value="6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF48">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="17">
        <location>
            <begin position="33"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="262"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="549" mass="62565" checksum="5B76EB6E0A927B04" modified="2024-11-27" version="1">MANLSMPSCLQRDAGLSSSADAKRAEPRANEKKCSWASYMTNSPTLIVMIGLPARGKTYMSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYKSYDFFRHDNEEAMKIRKHCALVALQDVKIYLNEEGGQIAVFDATNTTRERRDLILNFVKENAYKVFFVESVCDDPDVIAANILEVKVSSPDYPEAHRERVMDDFLKRIECYKVTYQPLDPEGYDKDLSFIQVMNVGRRFLVNRVQDYIQSKIVYYLMNIHVHSHSIYLCRHGESDHNMQGRIGGDSELSPRGKQFAAALRGFIEEHGLSDLKVWTSQLRRTIQTAEALGVPYEQWKILNEIDAGVCEEMTYEMIQSTFPEEFALRDQDKYHYRYPGGESYQDLVQRLEPVIMELERQGNVLVVCHQAVMRCLLAYFLDKSADDLPYLKCPLHTVLKLTPVAYGCKVELFYLNVEAVNTHRDRPLGKARTGPAPLLLRRNSYTPLSSHDQVKRPRLYSAGNRPWLPLAPSPSALHFPNTPEGVLLQSQPRVAGGCLNPCVKESTLTAQKKLLTASASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWI3</accession>
    <name>A0AAR2IWI3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Interleukin 2 receptor, gamma b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWI3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079880.1">
        <property type="protein sequence ID" value="ENSPNAP00000042409.1"/>
        <property type="gene ID" value="ENSPNAG00000007954.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164309"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053856">
        <property type="entry name" value="TSLPR_D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF42">
        <property type="entry name" value="CYTOKINE RECEPTOR COMMON SUBUNIT GAMMA ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22012">
        <property type="entry name" value="TSLPR_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Cytokine receptor-like factor 2-like" evidence="9">
        <location>
            <begin position="99"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="391" mass="44176" checksum="880EF80F2AE65D20" modified="2024-11-27" version="1">MNTSTMNSVFVTVGSTCFDDLIASMTSDEVVKALVDRGYTDVVLQVGRGSFVPDPQSCPGLRLEAFRFKESIAENIQLADLIISHAGKICTSQDCTVDINCMVINLEYVHCSWTRSQMQNMSYNFSSAFKHPDSYQECPQYLQENGQNVACRIPLHKNGQFDAFYTKLSMGGNLTVKKDYKHLKERVKINPPFNLSVNWSANGELCLYWVNNVSKPSCVEVMIRYRRASNQWQVSAEYQSTSYCIPLVPMGVNFTFQVKSRIGNLCGPGFWSEWSAPLQWGNYTGSISAPVPQSWWYLWLLGVIVLIALAVLLCYIERVKVLILPVVPDPSKNLQDLFYKHNGNVESWVHVSRELQEAFHTDYAESPCVVCEPSPTLKSKECEKPVQQHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYH8</accession>
    <name>A0AAR2IYH8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYH8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058092.1">
        <property type="protein sequence ID" value="ENSPNAP00000043112.1"/>
        <property type="gene ID" value="ENSPNAG00000032576.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164961"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="452"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="61906" checksum="4FB3703DC954ED34" modified="2024-11-27" version="1">MLGFSPVDERVASLRLRVGERVLTVVCAYAPNSSSEYPAFLESLGRVLESAPPGDSIVLLGDFNAHVGNDSETWRGVIGRNGLSDLNPSGVQFLDFCANHSLSITNTMFEHKDVHKCTWHQDTLGRSSMIDFVVVSSDLRPCVLDTRVKRGAELSTDHHLVVSWIRWWGKMPVRPGKPKRIVRVCWERLAEEPVRLIFNSHLRQNFDQISGEVGDIDSEWAMFRSSIAEAADCSCGHKVVGACRGGNPRTRWWTPQVRDAVKLKKESYRAWLACGTPEAAGRYRQAKRYAASVVAKAKTRVWEEFGEALESDFKSAPKRFWQTVRRLRRGKQCATSTVYSGDGVLLTSTEDVIGRWKEYFEDLLNPTDTFSSEEAESGDMGIGLSITEAEVAKVVKKLLGGKAPGVDEIRPEFLKALDVVGLSWLTRLFNIAWTSGAVPLDWQTGFVVPLFKKGDRRVCSNYRGITLLSLPGKVYAGVLEKRVRLIVEPRIQEEQCGFRPGRGTLDQLFTLSRILEGSWEFAQPVHMCFVDLEKAFDCVPRGILWEVLREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZG2</accession>
    <name>A0AAR2IZG2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Monocyte to macrophage differentiation-associated 2b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569523.1">
        <property type="nucleotide sequence ID" value="XM_017714034.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZG2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051571.1">
        <property type="protein sequence ID" value="ENSPNAP00000045090.1"/>
        <property type="gene ID" value="ENSPNAG00000032352.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108437129"/>
    <dbReference type="GeneTree" id="ENSGT00940000157972"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140911">
        <property type="term" value="F:pore-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005744">
        <property type="entry name" value="Hy-lIII"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01065">
        <property type="entry name" value="hlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF107">
        <property type="entry name" value="MONOCYTE TO MACROPHAGE DIFFERENTIATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26829" checksum="59B2678E7E7B6778" modified="2024-11-27" version="1">MNLVRFMNNRAPANKRYQPTEYEHAANCATHALWIIPSIVGGVLLYFLSDDHWEEIAAYLYGAGLSSLFIISTAFHTITWKKSHLRSVEQCFHMCDRMVIYFFIAASYTPWLTLRELGPWASHMRWVVWVMASGGTAYVFFFHERYKVVELVCYTAMGVFPAMVILSMADRSGLCELLVGGGCYVLGMVFFKSDGVVPFAHAIWHLFVAMGAGIHYYAIWKYLYTPVNQQTSTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0T8</accession>
    <name>A0AAR2J0T8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THAP domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0T8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044697.1">
        <property type="protein sequence ID" value="ENSPNAP00000043874.1"/>
        <property type="gene ID" value="ENSPNAG00000036774.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000197307"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001935">
        <property type="term" value="P:endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF2">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="64" mass="7114" checksum="67DE88ADBFC58746" modified="2024-11-27" version="1">MGGCSAPNCSNSTTIGKQLFRFPKDPVRMRKWLINCRRDFVPTPCSRLCQASGHNSLISAQSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J2J5</accession>
    <name>A0AAR2J2J5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SCG3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J2J5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000088083.1">
        <property type="protein sequence ID" value="ENSPNAP00000046190.1"/>
        <property type="gene ID" value="ENSPNAG00000036819.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267173"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000050">
        <property type="entry name" value="Titin isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000999">
        <property type="entry name" value="Uncharacterized protein, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF151">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="80"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="154"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="356"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="452" mass="50723" checksum="9723930396B1E55E" modified="2024-11-27" version="1">VRTETDGIKLICEVSKPSAEVVWYKGDEELPEGGRYEHIADGRKRVLLIQDLRMDDAGEYHCKLPSSQTTGTLRINELAAEFISRPQSQEVVEGEKAEFVCSVSKDSYEVRWLRGDKELQTDDKYEIISDGKRRVLVVKNCERNDEGHFVALIGATRASIVLNCEVNTEEAKAKWLKNDETLFESSKFIMVQRDTVFSLRIKNAEKSDEATYTITLTNQRGEYAKSSAKINYFFKHFVYRVDKDKHTLTIKDCSLADEGEYSVIAGSDKSTAELIITKAGTKIELPADITGKPEPKVKWTKADLILKEDDRITIDTKPGHSTVNIAKTTRDDSATYIVEAVNSILFFYISAGPPSPPQDLHVTEAARDHISVAWKAPERNGGSPVTGYHIELCEAGTEKWMRVNSRPKTSFKITNLAPGTEYYFRVIAVNKYGIGVPKDSPKSYLATDPMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J887</accession>
    <name>A0AAR2J887_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J887"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067575.1">
        <property type="protein sequence ID" value="ENSPNAP00000046527.1"/>
        <property type="gene ID" value="ENSPNAG00000036487.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271870"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="5"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="158" mass="18234" checksum="2D9DC7B5335CE8DF" modified="2024-11-27" version="1">MRNKILWSDETKIELFGVNARRHVWRKPGTAHHEANTIPTVKHGGGSIMLWGCFSAAGTGRLVRIEGKMNAAMYRDILDKNLLQSALDLRLGRRFIFQQDNDPKHTAKISKQWLQDHSVNVLEWPSQSPDLNPIEHLWRDLKMAVHRRLPSNLMDIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JAH6</accession>
    <name>A0AAR2JAH6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Retinoic acid receptor RXR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nuclear receptor subfamily 2 group B member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for retinoic acid that acts as a transcription factor. Forms homo- or heterodimers with retinoic acid receptors (rars) and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate gene expression in various biological processes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Heterodimer; with a rar molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JAH6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081533.1">
        <property type="protein sequence ID" value="ENSPNAP00000047342.1"/>
        <property type="gene ID" value="ENSPNAG00000035789.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159208"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06956">
        <property type="entry name" value="NR_DBD_RXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06943">
        <property type="entry name" value="NR_LBD_RXR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000002">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000005">
        <property type="entry name" value="Retinoic acid receptor RXR-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021780">
        <property type="entry name" value="Nuc_recep-AF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000003">
        <property type="entry name" value="Retinoid-X_rcpt/HNF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11825">
        <property type="entry name" value="Nuc_recep-AF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00545">
        <property type="entry name" value="RETINOIDXR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="122"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="220"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="191"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="467" mass="51403" checksum="D0350E83DDC4632C" modified="2024-11-27" version="1">LHFSQDSRSPDSSSVSSPPSGQRSPSLASSAAAMTSLPPITSAVNSPISSIGSPFSVISSSLGSPCLPGTPSVGYGPISSPQINSTVSMSGLHAVSSSDDVKPPYGLKQLSSHSPGPTLSQKRLCAICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLSYTCRDNKDCMVDKRQRNRCQYCRYQKCLAMGMKREDERRPREEERQRNKEREGEAESTSAVNEEMPVEKILEAEMAVEQKTEMHSDGSSGTSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSITVKDGILLATGLHVHRNSAHSAGVGAIFDRESAHNAEVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIILFNPDAKGLSSPSEVELLREKVYASLEAYCKQRYPDQQGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JK90</accession>
    <name>A0AAR2JK90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">UBE2V2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JK90"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060411.1">
        <property type="protein sequence ID" value="ENSPNAP00000050722.1"/>
        <property type="gene ID" value="ENSPNAG00000036244.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00740000115534"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="16"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="157" mass="17902" checksum="9E00B07CF7D4BF01" modified="2024-11-27" version="1">MSYMWIYVFITGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPARNCITSATNYENRIYSLKVECGPKYPEVPPTVRFVTKISMNGINNSNGMVDARSIPILAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLU2</accession>
    <name>A0AAR2JLU2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TERF1-interacting nuclear factor 2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DYNLRB2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLU2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051046.1">
        <property type="protein sequence ID" value="ENSPNAP00000051164.1"/>
        <property type="gene ID" value="ENSPNAG00000036260.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248783"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070187">
        <property type="term" value="C:shelterin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904356">
        <property type="term" value="P:regulation of telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039098">
        <property type="entry name" value="TINF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029400">
        <property type="entry name" value="TINF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15512">
        <property type="entry name" value="TERF1-INTERACTING NUCLEAR FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15512:SF0">
        <property type="entry name" value="TERF1-INTERACTING NUCLEAR FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14973">
        <property type="entry name" value="TINF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TERF1-interacting nuclear factor 2 N-terminal" evidence="1">
        <location>
            <begin position="20"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="72" mass="8255" checksum="3BE29B6389F1D46C" modified="2024-11-27" version="1">TNTPLLEVPCSIRVVAAEAWMVVRARDIKHFERVLEFLDVIHTLLPHLVTSIKHMKIIFGLKTLRSAYLMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JS84</accession>
    <name>A0AAR2JS84_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Carboxypeptidase N catalytic chain</fullName>
            <ecNumber evidence="17">3.4.17.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Carboxypeptidase N polypeptide 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Carboxypeptidase N small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">CPN1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Release of a C-terminal basic amino acid, preferentially lysine.</text>
            <dbReference type="EC" id="3.4.17.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Tetramer of two catalytic chains and two glycosylated inactive chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.3" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JS84"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047773.1">
        <property type="protein sequence ID" value="ENSPNAP00000053142.1"/>
        <property type="gene ID" value="ENSPNAG00000028447.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158235"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000011">
        <property type="entry name" value="Carboxypeptidase N catalytic chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000013">
        <property type="entry name" value="carboxypeptidase N catalytic chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF80">
        <property type="entry name" value="CARBOXYPEPTIDASE N CATALYTIC CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 23" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043703342" description="Carboxypeptidase N catalytic chain" evidence="23">
        <location>
            <begin position="26"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="24">
        <location>
            <begin position="30"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="21">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00083228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="451" mass="50775" checksum="7A39E4A2CD055D6F" modified="2024-11-27" version="1">KGEGWISLLWSGALLLGLWAQQAWAADDFQHHGYEEMVRALFSVQSQCPYITRIYSIGRSVEGRHLYVLEFSDNPGIHEALEPEFKYVGNMHGNEVLGRELLIYLCKFLCEEYIAGNQRITSLIHNTRIHILPSMNPDGYEVAARQGPEFNGYLVGRGNSRDVDLNRNFPDLNGLMYYYEKNNGKNHHLPLPDKWELQVEPETLAVIKWMQNYNFVLSANLHGGAVVANYPFDKSREARIRGKPTYSATPDDKIFKKLAKVYSFAHSWMHKGWNCGDYFEEGITNGANWYSLSKGMQDFNYLYTNCFEITLELSCDKFPPASALAREWLANREALVSYLEQVHHGIKGMVYDENNNPVSNAEISVAGVSHDITTGADGDYFRLLLPGTYTVTASAPGYQPSTSTVTVGPAEAIQLHFYLKPAPKVSNLNVRGHQGKKNLTAKVPPANLGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JSB4</accession>
    <name>A0AAR2JSB4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Usher syndrome type IIa protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Usher syndrome type-2A protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">USH2A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Photoreceptor inner segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000050830.1">
        <property type="protein sequence ID" value="ENSPNAP00000054905.1"/>
        <property type="gene ID" value="ENSPNAG00000021347.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158456"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032391">
        <property type="term" value="C:photoreceptor connecting cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000090">
        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000094">
        <property type="entry name" value="Laminin subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002683">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000275">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000313">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000111">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000126">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000819">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000915">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000991">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001004">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001030">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001037">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001085">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001099">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001100">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001168">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001176">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001211">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001227">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001285">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001379">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001716">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF7">
        <property type="entry name" value="USHERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="fn3"/>
        <property type="match status" value="18"/>
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        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="10"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
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    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="31"/>
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        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
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    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="28"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="4984"/>
            <end position="5006"/>
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        <location>
            <begin position="288"/>
            <end position="534"/>
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    </feature>
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        <location>
            <begin position="658"/>
            <end position="710"/>
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    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="711"/>
            <end position="763"/>
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    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="764"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="813"/>
            <end position="864"/>
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    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="865"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="17">
        <location>
            <begin position="968"/>
            <end position="1025"/>
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        <location>
            <begin position="1074"/>
            <end position="1166"/>
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    </feature>
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        <location>
            <begin position="1168"/>
            <end position="1261"/>
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    </feature>
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            <begin position="1263"/>
            <end position="1371"/>
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        <location>
            <begin position="1375"/>
            <end position="1480"/>
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    </feature>
    <feature type="domain" description="Laminin G" evidence="16">
        <location>
            <begin position="1529"/>
            <end position="1711"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="16">
        <location>
            <begin position="1716"/>
            <end position="1895"/>
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        <location>
            <begin position="1960"/>
            <end position="2056"/>
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            <begin position="2057"/>
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            <begin position="2147"/>
            <end position="2240"/>
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            <begin position="2241"/>
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            <begin position="2330"/>
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            <begin position="2493"/>
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            <begin position="2581"/>
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            <begin position="2776"/>
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            <begin position="2883"/>
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            <begin position="2978"/>
            <end position="3068"/>
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            <begin position="3364"/>
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            <begin position="3459"/>
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            <begin position="3547"/>
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            <begin position="3642"/>
            <end position="3732"/>
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            <begin position="3828"/>
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            <end position="4026"/>
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            <begin position="4027"/>
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            <begin position="4113"/>
            <end position="4224"/>
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            <begin position="4406"/>
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            <begin position="4494"/>
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            <begin position="4598"/>
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        <location>
            <begin position="4792"/>
            <end position="4892"/>
        </location>
    </feature>
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            <begin position="1359"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2665"/>
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            <begin position="5017"/>
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        <location>
            <begin position="2665"/>
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        <location>
            <begin position="681"/>
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    </feature>
    <feature type="disulfide bond" evidence="13">
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    <feature type="disulfide bond" evidence="13">
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        <location>
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    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
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    <feature type="disulfide bond" evidence="13">
        <location>
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        </location>
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    <feature type="disulfide bond" evidence="13">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080960"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082367"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054905.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
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    <sequence length="5140" mass="558357" checksum="880C54A11A144DDB" modified="2024-11-27" version="1">[encoded data, 5140 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JV54</accession>
    <name>A0AAR2JV54_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">BAR/IMD domain-containing adapter protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Brain-specific angiogenesis inhibitor 1-associated protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Participates in actin bundling when associated with EPS8, promoting filopodial protrusions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017561445.1">
        <property type="nucleotide sequence ID" value="XM_017705956.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JV54"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054687.1">
        <property type="protein sequence ID" value="ENSPNAP00000055885.1"/>
        <property type="gene ID" value="ENSPNAG00000022859.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432239"/>
    <dbReference type="CTD" id="678518"/>
    <dbReference type="GeneTree" id="ENSGT00940000153560"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07646">
        <property type="entry name" value="I-BAR_IMD_IRSp53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11915">
        <property type="entry name" value="SH3_Irsp53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000011">
        <property type="entry name" value="Brain-specific angiogenesis inhibitor 1-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000018">
        <property type="entry name" value="Brain-specific angiogenesis inhibitor 1-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030128">
        <property type="entry name" value="BAIP2_I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035594">
        <property type="entry name" value="BAIP2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027681">
        <property type="entry name" value="IRSp53/IRTKS/Pinkbar"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14206">
        <property type="entry name" value="BRAIN-SPECIFIC ANGIOGENESIS INHIBITOR 1-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14206:SF3">
        <property type="entry name" value="BRAIN-SPECIFIC ANGIOGENESIS INHIBITOR 1-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="IMD" evidence="19">
        <location>
            <begin position="7"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="391"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="305"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="549" mass="61215" checksum="12AADEA95A8EDA18" modified="2024-11-27" version="1">MVWTEDLAMSRTDEVHRITENVYKTIMEQFNPCLRNFVAMGKNYEKALSTVTFAAKGYFDALVRMGELASESQGSKDLGDVLFQMAEVHRQIQVQLEEMLKSFHTELLSELEKKVELDARYLTAALKKYQMEHKSKSESLEKCQAELKKLRRKSQGSKNPSKYNDKELQYVETISSKQSELDTFIAEGYKTALSEERRRYCFLVDRQCAVAKNSSAYHGKGKDLLTQKIPVWQQACSDPNKLPERAMLLVQQMASGSSTLLSSPLHSSKGNLVISDPIPGAQPLPVPPELAPFIGGGLGHQGRLMGPDGMSLVNGTTGLQGEDYWGDGGVSQGRPASPQPQSQPQPQRQVSDVYSNTLPVRRPQPAKNKASVGETRTLPRSSSMAAGLEKNGRSRVQAIFSHAAGDNSTLLSFTEGDIITLLVPEARDGWHYGENEKNKMRGWFPFSYTRVLPDSDNDKLKVNLHHGKSSSTGNLLERDDTSLPMPDYGLTARLLAQSLTQNRPRPYSMAGFTQPAIEDYDSHFAASDRSLEVYLRSNMMDDRTAPLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JV90</accession>
    <name>A0AAR2JV90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UBAP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017542085.1">
        <property type="nucleotide sequence ID" value="XM_017686596.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017542087.1">
        <property type="nucleotide sequence ID" value="XM_017686598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JV90"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059973.1">
        <property type="protein sequence ID" value="ENSPNAP00000055968.1"/>
        <property type="gene ID" value="ENSPNAG00000035270.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413886"/>
    <dbReference type="CTD" id="51271"/>
    <dbReference type="GeneTree" id="ENSGT00390000008092"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14315">
        <property type="entry name" value="UBA1_UBAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14316">
        <property type="entry name" value="UBA2_UBAP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1920:FF:000001">
        <property type="entry name" value="Ubiquitin associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1920">
        <property type="entry name" value="UBAP1 SOUBA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049467">
        <property type="entry name" value="UBAP-1-like_UBA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038870">
        <property type="entry name" value="UBAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042575">
        <property type="entry name" value="UBAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023340">
        <property type="entry name" value="UMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15960">
        <property type="entry name" value="LD44032P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15960:SF2">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22567">
        <property type="entry name" value="UBA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21267">
        <property type="entry name" value="UBAP-1_UBA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51497">
        <property type="entry name" value="UMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UMA" evidence="11">
        <location>
            <begin position="19"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="10">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="10">
        <location>
            <begin position="407"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="259"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="458" mass="49716" checksum="AC85C739E7B48C1E" modified="2024-11-27" version="1">MAARKSGSDIHNNGPISYLDDVPFKLNDKFRCPAKVGLPIGFCLPDCHSLLSDMQYDFSLEKLSARWGEDLAKVRADEAQAAELRRVESEKDRQAASQDSDAGLKAHPADEQDLLPPALNPVLAGLRHNAILTPLPAPSFGPAQPAPNNPAPQSLNLADFEREEDPFDKLELKTLDDKEELRSILQSQPQSSISAPEPPPPEPRPTSQSNTPPLQAKSALFHKPNGLIGLLDLERPGGGGVVATRGQIDAERPCNIRSLTFPKLSDPGDSPSEAPLNLYPAAPPRSLPNGTPPLLLRSIPHTNSSMHVELSGHAPNGTPKQSSPVPAAGPPSHPRPASEALLSLSPSERQCVETIVGMGYSYEGVLKAMQRQGQNVEQVLEYLFVHSRLCERGFDATAVEECLEMYQCSEEKALQFLQLMSQFGEMGFERDAIKEVLLVHNNDQDKALEDLMARAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K0A8</accession>
    <name>A0AAR2K0A8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP-1 complex subunit mu-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AP1M1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">Clathrin-coated vesicle membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K0A8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060199.1">
        <property type="protein sequence ID" value="ENSPNAP00000055551.1"/>
        <property type="gene ID" value="ENSPNAG00000025118.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157924"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09258">
        <property type="entry name" value="AP-1_Mu1A_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14835">
        <property type="entry name" value="AP1_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000002">
        <property type="entry name" value="AP-1 complex subunit mu-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000006">
        <property type="entry name" value="AP-1 complex subunit mu-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="10">
        <location>
            <begin position="168"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="446" mass="51118" checksum="B66EF3D89BBBDC67" modified="2024-11-27" version="1">MSASAVYVLDLKGKVLICRNYRGDVDMSEIEHFMTLLMDKEEEGTLSPILAHGGVRFMWIKHNNLYLVATSKKNACVSLVFSFLYKIVQVFSEYFKELEEESIRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGHKLDTGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFENTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPTDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDFGTHSLQNNGRYLLTMWPRGTGMQSHFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K0J3</accession>
    <name>A0AAR2K0J3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K0J3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078604.1">
        <property type="protein sequence ID" value="ENSPNAP00000055959.1"/>
        <property type="gene ID" value="ENSPNAG00000035441.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286941"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001684">
        <property type="entry name" value="zinc finger protein 33B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000286">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="375" mass="42164" checksum="246F46E9FE4C4E77" modified="2024-11-27" version="1">MASESSSAPSVHFPTVLTGFHNHVKKPPSHQEEGVTMVKPEEIKCEDVVSEISSTQTSSATLCTNPSPSITHDEEKMASCLESSPGPTVHLPAVLIYFNNHIKKRPNLQEGGTAMLKPEEIKSEDVVSEISSTQTSATLSTNPASRGTHDEEQMGLSSPAPSVQIPAVLIYFQNHTNKPPSHQEECNTVLKPEEIKNEDVVSEISITQTSTATPCINPSPRETPKNKDKGLTHQCSECEKSFTTPSHLQMHQRIHTAEKPYQCLWCEKSFTKPNNLQQHQRIHTGEKPFQCLDCGQNFSRQSHLKRHQHIHTGQKPSHCSECGQSFTCHSDLRRHQRIHARKRPYPCSECGHSFTRQNDLYRHQRFHTEKKHITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K837</accession>
    <name>A0AAR2K837_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K837"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071895.1">
        <property type="protein sequence ID" value="ENSPNAP00000060435.1"/>
        <property type="gene ID" value="ENSPNAG00000031579.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153212"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000194">
        <property type="entry name" value="UDP glucuronosyltransferase 2 family polypeptide a4 short isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF162">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 2 FAMILY, POLYPEPTIDE A1 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043534910" evidence="4">
        <location>
            <begin position="23"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="361" mass="40973" checksum="F29325381727C003" modified="2024-11-27" version="1">MIYRHLLPLVALLLIGSEISLSGNILVLPGEFSHWYNMRVIVDELVARNHSVTVLIHSASPSINFKQKENFKYIVFKVSIDKQHAEVLWQNMVNVSLNDTMTGIETLLFFWNMLSDFEEYLGSICEGMLLNQELIASLRESHFDVLLFDPVMPCSDLLAETLGLPLVMSFRLSFGYTFERLCGQLPTPPSYVPAAATQGHLNDHMNFIERVENVLLYVAATAVSKLQMMMFDKYYTKITGKPRTVCDTLGKVDIWLIRTYWDFEYPRPLLPSLKFVGGLQCKPAKPLPKEMEEFVQSSGDDGIVVFSLGSMVKTLSKQRANTIAAALGQIPQKVRDYTMKCLYLQKENQPSQGLQAILIWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KAN0</accession>
    <name>A0AAR2KAN0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-conjugating enzyme E2 G1</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E2 ubiquitin-conjugating enzyme G1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein G1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-protein ligase G1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KAN0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047984.1">
        <property type="protein sequence ID" value="ENSPNAP00000061335.1"/>
        <property type="gene ID" value="ENSPNAG00000016014.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165298"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23795">
        <property type="entry name" value="UBCc_UBE2G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000018">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="14">
        <location>
            <begin position="5"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="170" mass="19617" checksum="7D2325E94FEA69B9" modified="2024-11-27" version="1">FHLKCSILLRHLRWHELNKNPVEGFSAGLIDDDDIYQWEVVVIGPADTLFEGGFFKANLTFPHDYPLRPPKMKFITEIWHPNVAKNGDVCISILHEPGEDRFGYEKPEERWLPIHTVETIMISVISMLADPNSDSPANVDAAKEWREDPNGEFKRKVARCVRKSQEMAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KBU8</accession>
    <name>A0AAR2KBU8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="5">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GMPS</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the conversion of xanthine monophosphate (XMP) to GMP in the presence of glutamine and ATP through an adenyl-XMP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:11681"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KBU8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076432.1">
        <property type="protein sequence ID" value="ENSPNAP00000059922.1"/>
        <property type="gene ID" value="ENSPNAG00000014256.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006591"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000008">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000009">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000044">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000013">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000848">
        <property type="entry name" value="PRK00074.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11 20" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 20" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="21">
        <location>
            <begin position="224"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="251"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="700" mass="77845" checksum="9C2A6E4916CCC195" modified="2024-11-27" version="1">VGFMPALRHVSPIRSSHLDISGEPKDALCDYEGAVVILDAGAQYGKVIDRRVRELFVQSEILPLETPAFAIREQGFRAIIISGGPNSVYAEDAPWFDPAIFTIGKPVLGICYGMQMMNKVFGGTVHRKSVREDGVFSIGMDNSCSLFRGLQKEEMVLLTHGDSVDKVADGFKVVAQSGNIIAGIANEQKKLYGTQFHPEVDLTERGMDMLRNFLFEIAGCTSNFTVQNRQLACLREISEKVDKSKVLVLLSGGVDSTVCTALLNKALNQDQVIAVHIDNGFMRKRESQSVEEALTKLGIKLKVVNAAHTFYNGTTTLPISEEDRTPRKRISKTLNMTTNPEEKRKIIGDTFVKVANEVIGEMNLKPEEVYLAQGTLRPDLIESASHLASGKAEVIKTHHNDTELIRKLRDEGKVIEPLKDFHKDEVRALGRELGLPEEIVSRHPFPGPGLAIRVICADEPYVCKDFAETNNILKIITDFSASVKKPHTLLQRVKSCISDEEEEKLMQITSLHSLNAFLLPIKTVGVQGDSRSYSYVCGLTSKEAPHWESLMFLARLIPRICHSINRVVYVFGSHVKEPPTDITPTFLTTGVLSTLRQADFVAHSILRESGYSSKISQMPIILVPLHFDRDPLQKQPSCRRSVVIRTFITSDFMTGIPATPDNHIPEEVVLKMVSEIKKIPGISRVMYDLTSKPPGTTEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KBY6</accession>
    <name>A0AAR2KBY6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Armadillo repeat containing 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ARMC7</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017579815.1">
        <property type="nucleotide sequence ID" value="XM_017724326.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KBY6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047625.1">
        <property type="protein sequence ID" value="ENSPNAP00000061843.1"/>
        <property type="gene ID" value="ENSPNAG00000034630.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108443558"/>
    <dbReference type="CTD" id="79637"/>
    <dbReference type="GeneTree" id="ENSGT00940000164193"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042462">
        <property type="entry name" value="ARMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46263">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46263:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21864" checksum="1DC9962EA51011B5" modified="2024-11-27" version="1">MPSFPGMDRFCASERFEYLQGLVTEFQDTDSEEAKEQILANLANFAYDPRNMEALRMLQVTDLFLDMLTEENENFVEFGIGGLCNLSMDRECRDQILESGGIPLVTGCLSSHRDETVLSAITTLMNLTTGASRSETTESAVVQCMLRFSLTQNPRLSNLATVFLQDYCLEEQVDRARDIMQGHSQSVLGIPLPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KG78</accession>
    <name>A0AAR2KG78_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KG78"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081729.1">
        <property type="protein sequence ID" value="ENSPNAP00000061437.1"/>
        <property type="gene ID" value="ENSPNAG00000032727.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000278174"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="164" mass="18705" checksum="20547B6BF12167BD" modified="2024-11-27" version="1">MRDIGREFQRVRSSSIVFSKGIWFSRTRSNSLGEKFIPRYLMLPDCSDVGMMLILWSVFSVWIPLMFTFCLIFAASGSMKMINIEGESGQPCLVPLCRLKLCEIRSFVLTRAFGELYNVSIQFIKDGPNPNLCRTANRNVQFTLSNAFSASKETTIFSNLLMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KN85</accession>
    <name>A0AAR2KN85_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 177</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CFAP45</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KN85"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060022.1">
        <property type="protein sequence ID" value="ENSPNAP00000063566.1"/>
        <property type="gene ID" value="ENSPNAG00000036243.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164505"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029090">
        <property type="entry name" value="DUF4659"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33663">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33663:SF3">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 185"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15558">
        <property type="entry name" value="DUF4659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="210"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="69185" checksum="06B0C3F5C96EE1B8" modified="2024-11-27" version="1">MENSRSSSPLMHLDLNNFDSPEAELSRYVLTSPRSLESCARLGVKPIDLLFKTLTDFMEEHQDMPLEALTALHEVHERERRERLRLCREERDRIVQEEKEPVSAMKPFSALETVLEQASESQSSDSKVRFYREPARTGKRRSVVEFAQDFPKSPSEPCSSQLLPFSHSLGDLRHSPATVRRLGRLKQDICRKLSVTVPEKDCKIAALMLVKQEEEEARLQQSQLEELQREEAQRKEEARRALAERRRRKELLRSLRRWHEDFEARRRRREMEELQKARRQEWKTLEQEERWRRRAEEQEERRRGQLEMARREAEERRRRRDRLLEEAQRRKQAEQEEELEQAKLRERQARRSRSSRERREQKRLREDNQRERLRHSLLKQEVEEKAQAEEEAKRDALEQRLQRSVEKHARLLEARLEEMRERAQQEEARARRARLRAGWEQKERQQEKEVLVQLSLRRAKEAQRHVAEQCRRRAQQVKLENLEKEAGHRRLWEHVLKEGEVQQEQQKEAVALKERRREQLRREREEALERSRKVAHASCCMREMVREQSRSRTFQQMALQAELSARLSRMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KQT2</accession>
    <name>A0AAR2KQT2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFI6/IFI27 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KQT2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064531.1">
        <property type="protein sequence ID" value="ENSPNAP00000064471.1"/>
        <property type="gene ID" value="ENSPNAG00000037772.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000078591.1">
        <property type="protein sequence ID" value="ENSPNAP00000070562.1"/>
        <property type="gene ID" value="ENSPNAG00000031828.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287031"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009311">
        <property type="entry name" value="IFI6/IFI27-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038213">
        <property type="entry name" value="IFI6/IFI27-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932">
        <property type="entry name" value="INTERFERON ALPHA-INDUCIBLE PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932:SF37">
        <property type="entry name" value="ISG12-1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06140">
        <property type="entry name" value="Ifi-6-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="103" mass="9337" checksum="96B85272AB332F21" modified="2025-10-08" version="2">MLSVITVILKVILKPAGTVALAPAVLTAAGFTAGGIAAGSVASWMMSTAAVANGGGVAAGSAVAVLQSVGAAGLSGVATTAMASLGGVVGGAIAEAIAQNSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KRJ6</accession>
    <name>A0AAR2KRJ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017537277.2">
        <property type="nucleotide sequence ID" value="XM_017681788.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KRJ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044253.1">
        <property type="protein sequence ID" value="ENSPNAP00000066895.1"/>
        <property type="gene ID" value="ENSPNAG00000034898.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108410616"/>
    <dbReference type="GeneTree" id="ENSGT00940000163160"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000024">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="82"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="352" mass="38037" checksum="2CAA0850F4E78680" modified="2024-11-27" version="1">MSQKQNKEGKKIDEIGCRNTNHQLPYLTTTHGLEEDHQNRRRKRSMELHWWKALLITGAVLLNATGSLSQSVCGQAPFNNKIVGGETANSNAWPWQVSIQYRAGHFCGGSLINNNWILSAAHCFQSSNTNYAAALTIYLGMASLQGINSNSQIRSVSRIIIHPSYSTSSHDNDIALLQLTSSVTYTDYVQPACLAAAGSSLGGGTLVWVTGWGDIGNSVSLPFPQNLREVEVPVVTNSDCIASYGSSVTSNMICAGLTEGGKDSCQGDSGGPMVIKQDAGWTQVGIVSFGYNCAEPNFPGVYTRVSKYQNWINTYTSSNQPGYVTVITSSASLPLSLSLCITPFLFSLYMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KSU5</accession>
    <name>A0AAR2KSU5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuole membrane protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">VMP1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KSU5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080650.1">
        <property type="protein sequence ID" value="ENSPNAP00000067403.1"/>
        <property type="gene ID" value="ENSPNAG00000011637.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007230"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="46373" checksum="5B73692F6F60593C" modified="2024-11-27" version="1">MNETQTNCERPQRRAGVKEHRNGRSATKEADAASLSELKQRDKKERLTLVLWNRPALTLHYFLLEMFITLKEWIRRLWQQRGTAFAFLLLLFLFFITYSIEGAHQKYVRLLEKKCLWCAYWLGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVDFPEPPYPDQIISVIGTVAGDVSLWAIISKVRLEACMWGAGTAIGELPPYFMARAARLSGTEPDDEDYSEFEEMLEQTDTVQDFATRAKLTVQKMVQRVGFFGILACASIPNPLFDLAGITCGHFLVPFWSFFGATLIGKAVIKMHIQKLFVIITFSKHIVEQMVSLIGAVPLFGASLQKPFREYLETQRAKLHRAGETAPADENWLSWVFEKVVIIMVCFFVFSIINSMAQSYVKRIQQQKYSEGKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KWD1</accession>
    <name>A0AAR2KWD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ELFN2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with PPP1CA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KWD1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070808.1">
        <property type="protein sequence ID" value="ENSPNAP00000068653.1"/>
        <property type="gene ID" value="ENSPNAG00000030190.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159737"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099560">
        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000047">
        <property type="entry name" value="protein phosphatase 1 regulatory subunit 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055106">
        <property type="entry name" value="ELFN_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF204">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22986">
        <property type="entry name" value="Fn3_ELFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043636074" description="LRRCT domain-containing protein" evidence="14">
        <location>
            <begin position="34"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="15">
        <location>
            <begin position="196"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="454"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="603"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="816"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="491"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="548"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="829"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="898" mass="98399" checksum="AA5B296DF2072666" modified="2024-11-27" version="1">MKSKLYVLSSPCTLLLPLSCLFLLFSAPTPVSGDCWLIEGDKGYVWLAICSQNQPPYETIPQHINNTVHDLRLNENKLKAVFFSSLSRFTNLTDLNLTKNEISYIEDGAFAAQANLQVLQLGYNKLTNLTEGMLRGLGRMQCLFLQHNLIEVIATNTFWECPSLSSLDLSSNKLARLDPSTFTVLARLMVCELAGNPFHCGCELYSFLTWLEAFNNVTHTYDRLQCETPRELFGYPLLSPVGGHGRSARNILSSMCRDGVFIPGMTSLPPDSDSSGMDPNMFGPYHQPTTSSSSENSFSPSIKLQHVSLSSASLLVQIPRPYSKMYILVQYNKSYVSDVMNLKNKKELITLNKLKPNTNYTFCVASIRNSQRFNHTCLYFSTRAPTPDMLPNPSTTTHYIMTIVGCLFGMLIVLGLVYYCLRRRRRQEEKEKSICVKKTILEMRYGPEVAAAAASDPTTVQKLHEQTHHQHHHGKLPMSSSSSSGMLGHGSANTSSSRLSSIPQVEKMATAFSEAMASKGNYMDIRTSVGVEERVRDGVMQAVRAEDLREEDGSDLGDDSDDDGRGSASEISTIAMEVDKVNQIINNCIDALKLDSVVTAVSSATTTSGNPTSPPPTSTSSLTRGLIPLSTGVTEACQVMPSPKIPPPPPLPLTGPLSERPGITGGGFVSPPYRPPPPATAVRPIQRQMSADAAVIVSSSKKHCGPAGGKARVYSLDVPEPRSPDPCQYPEKGSPVGCGEPLERLPLVGSSGGGGGGCNMDGGTMDGMGQHLEVHPDYHCAEHRHSVPALYYEGSHDSPAQRASFLKPLSRAKRDAASYSQLSPRHHNYSGYSSSPEYSSESTLRIWERFRPYRKGPREESCYITAGNALRKKVQFAKGEDLHDILDYWKGVSAQQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L1W9</accession>
    <name>A0AAR2L1W9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L1W9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073389.1">
        <property type="protein sequence ID" value="ENSPNAP00000068627.1"/>
        <property type="gene ID" value="ENSPNAG00000033786.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165579"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14143:SF2">
        <property type="entry name" value="IMMUNITY-RELATED GTPASE FAMILY, Q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14143">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043669602" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="84"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="461"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="804" mass="87887" checksum="5845866FCB30CFA7" modified="2024-11-27" version="1">MRMQITVYVLLLMLLQKVTCHEEQQNKNGTHGSAGVTAVPEIKDEDFNRWQTDPSKNCSVIIDLISSNDAGFYQFRPQNKSQYPSIKVTIQEKPMILSPPLTDGRQATLNCSIPNPCPTAQPQITWKLGGNFTSLFNATTIVNLSNGDITSMLTFTPSFDLDGTVVQCVVKYGNTTTYTNWTLAVTRPNENIDRQNDQDSKVVQENTPLQGAQCDDNTDNLLTIRTVFSFLAGSSSTAIIACAVLCCVCICQRCGTLKATDGNTNIKLETVKIQPSQAVADNAGANEQTPLQKRRSEEARSNGSGGLEEEDTPGEDRNLGVHEAAGEETKEVDYACIDYSLIQKRQAEAQKPKSEDTEYAEIHIKKQAEGEGDELLQDGTDQSASRLEEGVESQEQEGEAESWSPALLHLRGDESCCVSMCGSTDMADVLKSLNLLESLKESIEKNNISDIKDAVEDMLISRINIAVVGERGTEKTMFLNSLRGLSVGDEGAALCPSAAAPEELAAFPNPKHPDFRLWDLPTISSDPTFDPENYMDRYKILRYNAIIMTFTQSPSTNMVAVWKEVRSLQKDTLYFALLASEKDTEKTLEAKKMASLEVLKAEGLSQPKVFLVCPAALEKLEFLKLLEVMQGDLPEIRAHALLLALPTFSSTLVAQKKEAFKALGWAAASLSAGVSAIPVPLVASMVDASVAVRILTKAQISLCLDDESVERLAKQRGIEPNKLKGLRTCLLSVEVSKSEVKRRLSVAEKDTTTATTKLVELAMPRHARSVSRSFTVMLQAMNSAIDDMSADADKIVTIATGVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L937</accession>
    <name>A0AAR2L937_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TCTP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TPT1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in calcium binding and microtubule stabilization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCTP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570597.1">
        <property type="nucleotide sequence ID" value="XM_017715108.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L937"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053838.1">
        <property type="protein sequence ID" value="ENSPNAP00000071167.1"/>
        <property type="gene ID" value="ENSPNAG00000036734.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108437769"/>
    <dbReference type="CTD" id="7178"/>
    <dbReference type="GeneTree" id="ENSGT00390000006051"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.10:FF:000001">
        <property type="entry name" value="Tumor protein, translationally-controlled 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018103">
        <property type="entry name" value="Translation_control_tumour_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01003">
        <property type="entry name" value="TCTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="171" mass="18979" checksum="D4FFCB420B7C4D2E" modified="2024-11-27" version="1">MIIYKDIITGDEMFSDIYKIKESENGMMIEVEGKMISRAEGDIDDALIGGNASAEVLDDGCESTTVSGVDIVLNHKLSETSYDKKSYTAYIKDYMKAVKAKLQETAPNRVEPFMANAPAEVKKILANIKNYQFFTGESMNPDGAIGLLDFRADGQTPFMLFFKDGLEIEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L9R5</accession>
    <name>A0AAR2L9R5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037395097.1">
        <property type="nucleotide sequence ID" value="XM_037539200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L9R5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073095.1">
        <property type="protein sequence ID" value="ENSPNAP00000071126.1"/>
        <property type="gene ID" value="ENSPNAG00000033492.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413097"/>
    <dbReference type="GeneTree" id="ENSGT00940000165332"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002239">
        <property type="entry name" value="Zinc finger protein 226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF54">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF26.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="241"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="293" mass="33459" checksum="7A084D8EF9CE0A4D" modified="2024-11-27" version="1">MQPARRSAVLHRRSHAGEKPHTCLTCSKTFISSTHLALHLRTHAGERRYKCSVCNKVFTQPAHLMRHKAIHTGEKPFKCPDCGKSFGRASHLKTHQRLHTGEKPYKCTLCEKAFTQKAGLIMHLRQHTGERPYKCEKCGKGFRSSAHLLSHQALESGEGRHVCVKCRKSFRSVSVLRQHEKSHQGGAVEALRCSVCSDSFVCSPYLRLKLRLRPAECIYHCKVCNRMFDKMSIFERHCEKHREQPDSDVEEEGEKEEDTGDPLFLPHGADFTYPSEVNTRSKTRAKTKLEPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LME1</accession>
    <name>A0AAR2LME1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Chromatin assembly factor 1 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Chromatin assembly factor I p60 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CHAF1B</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017562054.1">
        <property type="nucleotide sequence ID" value="XM_017706565.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017562055.1">
        <property type="nucleotide sequence ID" value="XM_017706566.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017562056.1">
        <property type="nucleotide sequence ID" value="XM_017706567.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LME1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048045.1">
        <property type="protein sequence ID" value="ENSPNAP00000077730.1"/>
        <property type="gene ID" value="ENSPNAG00000007990.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432609"/>
    <dbReference type="CTD" id="8208"/>
    <dbReference type="GeneTree" id="ENSGT00550000074968"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000248">
        <property type="entry name" value="Chromatin assembly factor 1 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003156">
        <property type="entry name" value="Chromatin assembly factor 1, subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029129">
        <property type="entry name" value="CAF1_p60_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15512">
        <property type="entry name" value="CAF-1_p60_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="18">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit B C-terminal" evidence="17">
        <location>
            <begin position="412"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="391"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="473"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="495"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="586"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF15512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="613" mass="67075" checksum="C0C046E633FDECBC" modified="2024-11-27" version="1">MKVITCEIAWHNKEPVYSLDFQQNADGKIDRLATAGVDTTVRMWRVDRGPDGKAVVEFLSNLARHTKAVNVVRFSPASDLLASGGDDAAILLWKLNDSKEPEQAPAFQEDEDAQLNKESWNVVKTLRGHIEDVYDISWTSDGNFLVSGSVDNTAIMWDVQKGQKMCIFNDHKSYVQGVAWDPLGQYISTLSCDRVMRVYSTHAKKKAYSVSKMTSGSAVEGEVKNYRMFHDDGMRSFFRRLTFTPDGSFLLAPAGCVEAGENVTNTTYVFSRKSFKRPIAHLPCPAKATLAVRCCPVYFELRTKKAEDGSLQPLPNTFKLPYRLVFAVASEDSIFFYDTQQALPFGYASNIHYHTLSDLSWSRDGSFLAVSSTDGYCSFVSFDERELGTPLKERPPLDIITPVSASEKKGKRTPATGRTSSPVPRPTPMPVTQEKEKEASSASVPSKIPGAPVPEEKRTPLGLKAKPQPRRITLNTLEGWSKPSTPKSPAIITGAPTSSTSMPKSAPSTPLSSHGSTTPKTAPSTPLGPVNVKNTTTPKGPTPRRISLMPVRSITSSPTEKAKHERPSPPNDPVCKPPESKRPKTNETPAHNDGTSTSAQSSEPSVDSKPTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LQF4</accession>
    <name>A0AAR2LQF4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017568692.1">
        <property type="nucleotide sequence ID" value="XM_017713203.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LQF4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055787.1">
        <property type="protein sequence ID" value="ENSPNAP00000078805.1"/>
        <property type="gene ID" value="ENSPNAG00000027534.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436607"/>
    <dbReference type="GeneTree" id="ENSGT00940000164876"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF115">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="381" mass="44267" checksum="96A054502B0D9649" modified="2024-11-27" version="1">MSTFSLSRLRFQSCFILLLSITFTVSVIIYVFGFHTELSKVWFHKFAKLIRFQKVKNHTLINMTCLTRHNVTDMLSMESMTAFPLNEASSICYHEAYPHNYGFIIDEPDKCEQENPFLVLMVPVAPHELEARNAIRSTWGNERVVQNKNITVVFSLGLPGIEAEKQQEELRLESQQYHDLIQSDFMDTYINLTIKTMVIMDWLATRCPQASYAMKIDSDMFLNLENLMSLLLRPDTPKENYMTGKVMWNRPVIRDKNSKWYVPKELYDEDYYPTYLLGMGYVFSNDLSRKIVEVSKEIKPFNIEDAYVGACVEQIGISPSDPPNPFQFKDYFSGKYKREEFASVITTILHSPQQLITFWQDLKGPHEGLEGKRTDCALLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LQW2</accession>
    <name>A0AAR2LQW2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein 54a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM54 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563283.1">
        <property type="nucleotide sequence ID" value="XM_017707794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LQW2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086320.1">
        <property type="protein sequence ID" value="ENSPNAP00000078960.1"/>
        <property type="gene ID" value="ENSPNAG00000032507.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433317"/>
    <dbReference type="CTD" id="393318"/>
    <dbReference type="GeneTree" id="ENSGT00390000004700"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020977">
        <property type="entry name" value="Beta-casein-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31258">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31258:SF5">
        <property type="entry name" value="TMEM54 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12304">
        <property type="entry name" value="BCLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="215"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="26870" checksum="AA37E6CAA64C582C" modified="2024-11-27" version="1">MGLCCRSLKDEKILMKMGLGLVLAGHLNFLLGALVQGAVLRDVKVSSQFATIEYAISNIIALVAGLTAIIGGISAIVLSKNMKNQPLKWSLLVMSILAFFFGSASAVSVTVSMVTAISNSGMSLLTQCNLPGNISAYNSYRVTNECPFDPTRIYGTTLILWVLLIVMSMVEMVFSGRCFVASTAFLRLPCPWRKRAVNASRVRFRIPESVPASPVTVQHIDGPEEGEPSEEHELLDAATSPKQTSDWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LTL1</accession>
    <name>A0AAR2LTL1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BIRC7</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_037396907.1">
        <property type="nucleotide sequence ID" value="XM_037541010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LTL1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069031.1">
        <property type="protein sequence ID" value="ENSPNAP00000078074.1"/>
        <property type="gene ID" value="ENSPNAG00000032751.1"/>
    </dbReference>
    <dbReference type="GeneID" id="119264005"/>
    <dbReference type="GeneTree" id="ENSGT00940000164164"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043027">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd16713">
        <property type="entry name" value="RING-HC_BIRC2_3_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14396">
        <property type="entry name" value="UBA_XtBIRC7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000005">
        <property type="entry name" value="Baculoviral IAP repeat containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000184">
        <property type="entry name" value="Baculoviral IAP repeat containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000002">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000084">
        <property type="entry name" value="E3 ubiquitin-protein ligase XIAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048875">
        <property type="entry name" value="BIRC2-3-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050784">
        <property type="entry name" value="IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF163">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044">
        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21290">
        <property type="entry name" value="UBA_BIRC2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01282">
        <property type="entry name" value="BIR_REPEAT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0789">Thiol protease inhibitor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="370"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="316"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="417" mass="45729" checksum="4564AA6BFF415002" modified="2024-11-27" version="1">MASGGFAPRLDGPLWTESGMSGTGEQHGGARRPGMRSEEERLRSFQLWPPGAPVTAADLARAGFYFLDHEDSVQCFCCAGVLRCWVRGDEPLHEHRRHFPTCRFVLGRDVGNIPRPPGPPDSVDGQLLSQLQRMGVDEQMAGGQAVYPEMESEDTRLTTFHNWPTGASVQPTTLARAGFFYTGHGDNVKCFFCDGGLRNWEPGDDPWQEHAKWFPRCEYLLQSRGQEYVNNIQQSYFNTPETGGSETSAAADITSGHEVLSGQSAVATAMLSPVVQTVLQMGFQQALVESLVQSRYLLTGQHYTSVSDLVADVLQAEEEERQGSESRPEPVVRQGGSAGGVRTQASVGEKVLGNLSAEEQLRQLQEERTCKVCMDKLVSMVFIPCGHLVVCTDCAASLRHCPICRAVIRGSVRAFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LYH5</accession>
    <name>A0AAR2LYH5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Beta-enolase</fullName>
            <ecNumber evidence="4">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:10165"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LYH5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067186.1">
        <property type="protein sequence ID" value="ENSPNAP00000079376.1"/>
        <property type="gene ID" value="ENSPNAG00000019085.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182805"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF5">
        <property type="entry name" value="BETA-ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="18">
        <location>
            <begin position="25"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="17">
        <location>
            <begin position="149"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="377"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="441" mass="47839" checksum="ABADEBA1371AE695" modified="2024-11-27" version="1">MLDVNDETGAAQTLLPYPRPQRPAGVTDSNACVSAGRFRAAVPSGASTGVHEALELRDGDKSRYLGKGTQKAVDHVNKELAPKLTEQKLSVVEQEKIDKLMLDLDGTDNKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNRDVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFSEAMRIGAEVYHNLKNVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIEKAGYPDKIIIGMDVAASEFHKAGKYDLDFKSPSDPQRYITGDQLGDLYKSFIKNYPVMSIEDPFDQDDWENWAKFTGSVDIQIVGDDLTVTNPKRIQQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGEKAKYAGKDFRHPKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M690</accession>
    <name>A0AAR2M690_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M690"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087032.1">
        <property type="protein sequence ID" value="ENSPNAP00000082877.1"/>
        <property type="gene ID" value="ENSPNAG00000030643.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286905"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000013">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF391">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="40"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="318" mass="36078" checksum="BF18FAA6BD8E67AE" modified="2024-11-27" version="1">ILKPVGEQSAFKLSGFHELGEWRPFLFIPYFLMFLLSTLSNSILIYLIVSKRALHSPMCVLIGLMAVVDLSLPIFFVPNMLLSFLFNWNGISLVGCLIQMFCLHYVGTFQSTLLAWMAVDRFFAICRPLYYHKHMEIPTFLKFIIVPLIRNVLLIVTIVSLAGKLTFCVTDEIDHCFCEHMALVQLACGDISMNNLVGLLAAFLIPTVDFIFFTLSYIIMLASVLKSGKSHLKAINTCVTHIIVMAVTLTFALIAFLSYRIRNNFSSSTRVFLSTMYLLFPSCFNPIIYGVRTKEIREQFLKCMNQIKVLPQCCNAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQC6</accession>
    <name>A0A3B4BQC6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calponin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575708.1">
        <property type="nucleotide sequence ID" value="XM_017720219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQC6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001912"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011351.2">
        <property type="protein sequence ID" value="ENSPNAP00000001912.1"/>
        <property type="gene ID" value="ENSPNAG00000001008.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440984"/>
    <dbReference type="CTD" id="572250"/>
    <dbReference type="GeneTree" id="ENSGT00940000159680"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000040">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF12">
        <property type="entry name" value="CALPONIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="28"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="298" mass="33601" checksum="48C63C0AFA92B7C5" modified="2018-12-05" version="1">MAQQHFRSGPAFGLSAEVKSKLAQKYDPQKEEELRLWIQEVTGRKVPDNFMEGLKDGVILCELINALQPGSVKKINNSTQNWHQLENIGNFVRAIQEYGLKPHDIFEANDLFENVNHTQVQSTLIALAGMAKSKGFHSKYDVGVKYAEKQQRRFAPEKLKEGRNVIGLQMGTNKFASQKGMTSYGTRRHLYDPKVGMENPLDQSTISLQMGTNKGASQSGMAAPGTKRQIFNKKLDMESCDTSIISLQMGTNKVASQSGMTVYGLPRQVYDRKYCTYPEDYMDNGQDMREIDGYQYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BR11</accession>
    <name>A0A3B4BR11_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Potassium inwardly rectifying channel subfamily J member 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563178.2">
        <property type="nucleotide sequence ID" value="XM_017707689.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037403567.1">
        <property type="nucleotide sequence ID" value="XM_037547670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BR11"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002058"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011085.2">
        <property type="protein sequence ID" value="ENSPNAP00000002058.2"/>
        <property type="gene ID" value="ENSPNAG00000008539.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433247"/>
    <dbReference type="CTD" id="556608"/>
    <dbReference type="GeneTree" id="ENSGT01140000282491"/>
    <dbReference type="OMA" id="PHCVHCF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000036">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000002">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003268">
        <property type="entry name" value="K_chnl_inward-rec_Kir1.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF6">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01321">
        <property type="entry name" value="KIR11CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 15" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="17">
        <location>
            <begin position="24"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="18">
        <location>
            <begin position="176"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="14">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002058.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42516" checksum="812738538A159DDE" modified="2024-11-27" version="2">MLQFIQKQIHGHLVERRIRRTRLVTKDGHCNIEFGNVKYHSHFAYLMDFWTTFVEIRWRFVIFSFVAAFTGSWFVFGLLWYSIAKSNGDLEAVEGEHSADGHAKCIDNINGLTTAFLYSLETQTTIGYGGRALTGHCAGTVALLVIQSLVGAIINCFMCGLILAKISLPKKRAKTVTFSDTAVICLKKGSLCLQIRVANLRKTLLIGSQIYGKLLRTTISSDGEPIILDQVNIDFLVDAGKDNLFFVCPLTLYHVIDSSSPFAEMAADTILQQEFELVVFLEGVAESTSSSCQVRTSFIPSEIQWGYSFLPIISRTKGGKYRVDFSNFSKTVPVVTPHCACCYLNELNSSLILHQHGQQGIDNLGFDVSSITIEDSMDNTQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRW9</accession>
    <name>A0A3B4BRW9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Solute carrier family 12 member 7a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) + chloride(in) = K(+)(out) + chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:72427"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the SLC12A transporter family. K/Cl co-transporter subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574692.1">
        <property type="nucleotide sequence ID" value="XM_017719203.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRW9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002378"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010520.2">
        <property type="protein sequence ID" value="ENSPNAP00000002378.1"/>
        <property type="gene ID" value="ENSPNAG00000008807.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440363"/>
    <dbReference type="GeneTree" id="ENSGT00940000157657"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000049">
        <property type="entry name" value="Solute carrier family 12 (potassium/chloride transporter), member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000040">
        <property type="entry name" value="Solute carrier family 12 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000076">
        <property type="entry name" value="KCL_cotranspt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00930">
        <property type="entry name" value="2a30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF47">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01081">
        <property type="entry name" value="KCLTRNSPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="194"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="450"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="550"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="574"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="608"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="18">
        <location>
            <begin position="121"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="18">
        <location>
            <begin position="407"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="703"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="833"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="1026"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1116" mass="124329" checksum="62E657BEDBFB8709" modified="2018-12-05" version="1">MPTNFTVVPVEDGQTSSPDSDDKHSGGEDEDKPGLYSGDETPGEDSPFLTISDHERSCDGNNMALFEEEMDSTPMVSALLNKLANYTNLTQGVVDHEKAESEDGVKRVAIKGPQMGTFIGVYLPCMQNILGVILFLRLTWIVGTAGILGSFTIVFMCCACTLLTAISMSAIATNGVVPAGGSYYMISRALGPEFGGAVGLCLYLGTTFAGSMYILGTMEILLMYIVPDGALFADKMDEPMSRQNNMRVYGSCCLALMALMVFVGVKYVNKLALVFLSCVILSIMAIYAGVIRTVIDPPEFQVCLLGNRTLKNLDFDKCLKTEVINNSTHTTELWGLFCDGPYLNASCDDYFTFNNVTEIKAIPGLLSGVISENLWGDYGPSGRLIEKNLSSEATQETFEDMKKPYVFSDITTYFTLMVGIYFPSVTGILAGSNRSGDLRDAQRSIPIGTILAIVTTSIIYMSCVVLFGACIEGVVMRDKFGYSLKRTPVIGTLAWPSPWVLVIGSFFSCCGAGLQSLTSAPRLLQAVARDGIVPFLKVFGHSKSNGEPTWALLLTAGICEIGILIGSLDFVAPVLSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWSLSFLGMSLCLSLMFVSSWFYALVGMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALIKLEEAPPHTKNWRPQMLVLLTLDSDLAVKHPRLLSFTSQLKVGKGLTIVCSVLQGTFMTRSADAKRSEENVKKSMAAEKTKGFCHVVVCSNLRDGFSNLIQSAGLGGMKHNTVLMAWPSNWRQAEDTSSWKNFIETVRETTAAHQALLVAKNIDHFPCNTERLVEGTIDVWWIVHDGGLLMLLPFLLRQHKVWKKCKMRIFTVAHLDDNSIQMKKDLHMFMYQLRLDAEVEVVEMHASDISAFTYEKTLVMEQRSQMLKQMQLSKTEREREIQSITDESRSSIKRKNCMKEPTPNTLEVPGMAEIENEAQMFHERNTKPHDGHQEKHSPTSDHIHMTWTKEKFISERNRHREASPGIRDIFNMKPEWEHLNQSNVRRMHTAVKLNEVVVNKSQGAHLVLLNMPGPPKNKGGDENYMEFLEVLIEGLDRVLLVRGGGREVITIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSJ1</accession>
    <name>A0A3B4BSJ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017541120.1">
        <property type="nucleotide sequence ID" value="XM_017685631.2"/>
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    <dbReference type="STRING" id="42514.ENSPNAP00000001490"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012072.2">
        <property type="protein sequence ID" value="ENSPNAP00000001490.1"/>
        <property type="gene ID" value="ENSPNAG00000000782.2"/>
    </dbReference>
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    <dbReference type="CTD" id="563577"/>
    <dbReference type="GeneTree" id="ENSGT00940000153463"/>
    <dbReference type="OrthoDB" id="10038642at2759"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:p53 binding"/>
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        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="ASPP1/2"/>
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            <begin position="1056"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVR1</accession>
    <name>A0A3B4BVR1_PYGNA</name>
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        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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        </reaction>
    </comment>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="Ensembl" id="ENSPNAT00000010194.2">
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        <property type="gene ID" value="ENSPNAG00000008945.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000001830"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:photoreceptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:protein deubiquitination"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ataxin 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ataxin-3 isoform X1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S15/NS1, RNA-binding"/>
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        <property type="entry name" value="Ataxin-3"/>
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        <property type="entry name" value="UIM_dom"/>
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    <dbReference type="PANTHER" id="PTHR14159">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14159:SF0">
        <property type="entry name" value="ATAXIN-3-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="SUIM_assoc"/>
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        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01233">
        <property type="entry name" value="JOSEPHIN"/>
    </dbReference>
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        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="15">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="258"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="active site" evidence="12 13">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR633865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="341" mass="38280" checksum="00654EB061A9FDFE" modified="2018-12-05" version="1">MESIFHEKQEGSLCAQHCLNNLLQGEYFTPVDLSSIAQQLDEEERIRMAEGGVLSEEYRTFLQQPSGNMDDSGFFSIQVISNALGVWGLELILFNSREYQRLMIDPVNERAFICNYKEHWFTVRKLGQQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVIRGNLPECEADQILEIMRVEQQHRPKLIGEDEAQTSSGQRTSGQHALETRHGLEESVMDEDEEELRKALALSRQDMEVEDEEADLRRAIQLSMQGASGSSSLIGTKEVGATMTPGSSGSTTGGVAQSTPSESLTAEELRRRRQAYFDRQSQQQAQPTSSQDTKVAGEEQDTGSKHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWL1</accession>
    <name>A0A3B4BWL1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Adenosine A3 receptor a.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWL1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007740.2">
        <property type="protein sequence ID" value="ENSPNAP00000004013.2"/>
        <property type="gene ID" value="ENSPNAG00000001396.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234555"/>
    <dbReference type="OMA" id="WHENETF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001609">
        <property type="term" value="F:G protein-coupled adenosine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001634">
        <property type="entry name" value="Adenosn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF54">
        <property type="entry name" value="ADENOSINE RECEPTOR A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00424">
        <property type="entry name" value="ADENOSINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="27"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU201114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004013.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="316" mass="35775" checksum="24CA23ECEACF1396" modified="2024-11-27" version="2">VKISNKYSAVWVFLPHEVIIGITCCMGNALVIWAVWTSLRQPTFCFIVSLAVADFLVGSVAIPIAVLVDIGMNIPFYGCLFMCCVIIMLQVASVVLLLAIAVDRFLRVRIPLTYKSTVSKKRLWIVVALCWVSAALLSFIPMFGWHGSLGADRIQCSFLTVISTSYVVNFIFFSLYLPPLALMTGLYCYIFLTTRRQLRANIGVAAESRTYYQKEHRLAASLVLVLVLFVVCWLPVFLMFTVTLYSPNIEVPYVAIDIGVLLSHAHSAVNPIVYAFRIPKIKEACRNLWRRVYHDREQQSDQNNTQNNTDRRQTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2B7</accession>
    <name>A0A3B4C2B7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017568166.1">
        <property type="nucleotide sequence ID" value="XM_017712677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2B7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005923"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004448.2">
        <property type="protein sequence ID" value="ENSPNAP00000005923.2"/>
        <property type="gene ID" value="ENSPNAG00000002064.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436280"/>
    <dbReference type="CTD" id="572323"/>
    <dbReference type="GeneTree" id="ENSGT00940000155356"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042771">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14211">
        <property type="entry name" value="STKc_HIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-interacting protein kinase 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000022">
        <property type="entry name" value="Homeodomain-interacting protein kinase 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF126">
        <property type="entry name" value="HOMEODOMAIN-INTERACTING PROTEIN KINASE 1-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="179"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="76"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="785"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="942"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="785"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="949"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005923.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1110" mass="120125" checksum="120C83F63BA461F4" modified="2024-11-27" version="2">MASQLQVFSPPSVSSSAFCRVKKMKVESCAWDASSEAYGSVGQAYGFNTAPALPFSASGLVFPPASRCQVVVRAADSTGGAPQGSSRRPTESHASRSQRYGVKRKNEEVDRSGGGDSGSGSVQILEELSAPAYSARAAGGNTAQSIPHSAPTTKSSSSNCEGDYQLVQHEILCSVSSSYEVLEFLGRGTFGQVAKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILNRLSAENADEFNFVRSYECFQHKGHTCLVFEMLEQNLYDFLKHSKFSPLPLRHIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPIRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFHRGPDSSYPLWRLKTPSEHEAEMGIKSKEARKYIFNCLDDMMQVNLSSHLEGTDMLAEKADRREFIDLLKRMLRLDADKRITPTKTLAHPFVTMSHLLDFPHSSHVKSCFQNMELCKRRNGSYDNGKALFAANTVPGTTGNLTVTFSSQLNQNNQVQVPSAGGAVPLLNYQPALYQQATINIPGLAQPSIPLQSRPPQLCTQTEPFQQTLIVCPPTTIQGLPSSSKTSSYPVRMENGVPVVQQSQNTQPLQIQPGMLAQGSCTPLMVATLHPHAPGVPSQYSLPLALGCGAGRPSLLEQTATVLQAWQAGTQQILLPPAWQQVPGMTLHGTGTAQTITESPLETILSDSSTQQTPNWRASHSTVLQQNPHVSNSTQSLNRGASSTARSSQSKRNKARCLDSSSGLVGTPYNSAALSQPIVISDTPSPAVSVITIHSDSDDEDERKFHPASCGPSQRTNVISCVTVHDSDSSTASPLTPLPRNSLAAQPSRLVKSLAVVAPSVKTQPGESSALAKVATGVLQSSYIKPKRAATRQPCSSGDSVNLQEPSRSQPLNLSQTTVSSSQDHTSGSSLRRQQTYPPSSSQYRLQEALPFTSAPSLYTYPASAALASASHTMEQLLVQGSSPSIHVPPTSHYAASLLPKDTVGGVVHGLPAHYQQHFSTHPYVGTNTSSTRGGAAYGGYQLSPRRVTQYPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2V7</accession>
    <name>A0A3B4C2V7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras protein-specific guanine nucleotide-releasing factor 2a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017549198.1">
        <property type="nucleotide sequence ID" value="XM_017693709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2V7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006098"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004152.2">
        <property type="protein sequence ID" value="ENSPNAP00000006098.1"/>
        <property type="gene ID" value="ENSPNAG00000002184.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425214"/>
    <dbReference type="CTD" id="558859"/>
    <dbReference type="GeneTree" id="ENSGT00940000155679"/>
    <dbReference type="OMA" id="GYASMHT"/>
    <dbReference type="OrthoDB" id="10254377at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13261">
        <property type="entry name" value="PH_RasGRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000005">
        <property type="entry name" value="Ras-specific guanine nucleotide-releasing factor 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000117">
        <property type="entry name" value="ras-specific guanine nucleotide-releasing factor 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000176">
        <property type="entry name" value="ras-specific guanine nucleotide-releasing factor 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000004">
        <property type="entry name" value="ras-specific guanine nucleotide-releasing factor 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF359">
        <property type="entry name" value="RAS-SPECIFIC GUANINE NUCLEOTIDE-RELEASING FACTOR 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="22"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="7">
        <location>
            <begin position="240"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="467"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="8">
        <location>
            <begin position="632"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="6">
        <location>
            <begin position="1039"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="740"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="148"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="762"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="791"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1276" mass="145038" checksum="2E085BFDA736F920" modified="2018-12-05" version="1">MQKSVRYNEGHALYLSVIARKEGVKRGYLSKKTAENSRWNEKYFALYQNVLFYFENEQSARPTGIYLLEGCTCERVPAPKVSAVGRDSSDKQQHYFLVIFGHDGQKPLELRAEEDADCSEWVEAIQQASYSDLIIEREVLMQKYIHLVQILETEKVAANQLRTQLEDQDTEIERLKAEIIVLNKTKEKMLPYQSNQEEEDPDIKKIKKVQSFMRGWLCRRKWKLIVQDYICSPHAESMRKRNQIVFNMVEAETEYVLQLSILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYESNAACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSKMMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFVRQGSLIQLPSAEKGKLSKVRLGSLSLRKEGERQCFLFTKHLLICTRTSGGKLHLLKQGGTLSLIECTLIEELDANDEDYNAAGQGFSHLEFKVVVEPVDGPSFAVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTSVFEENSKVTVPHMLKSDIRLHKDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVMDKLADIYKKPFTSIPVRIQYHSSDRLSISSICPDYSLKITRLALDQSKSLELFFATNQGPWGSEHLNNKSPRLSRKFSSPPPLSIPSRTSSPVHSRKLSLSSPVGGGKAGALDLSAASSPSAASSPTSTYNPVISSPPPTCTKAPLDLSRGPSSPELVPTTPEEGGELPRIDAFCGKLRRSIRRAVLESVSLDKFIPETPTSVEVGDMSPCRSPSTPRHLRYRQAGGQSTENSRCTMSPASAFAIATAAAGHSGPQGFANLEKSYDKEFLIRRAATNRVLNVLRHWVSKHSQDFDMNGELKTGVITLLEEVLRDPDLLPQERKATANILSALSQEEQDDSQLKIEDILQMAEYPKTECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKVDKNEKTPYIMKTSQHFNDMSNLVASQIMIHTDVSSRASSIEKWLAVADICRCLNNYNGVLEITSALNRSAIYRLKKTWAKVCKQTKALMDRLQKIVSSEGRFKNLRETLKNCNPPCVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQAPYRIEHQPKVTQFLLDKTFVMDEDTLYELSLKIEPRLPPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5H3</accession>
    <name>A0A3B4C5H3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Acetylcholine receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-1/CHRNB1 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5H3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006979"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002649.2">
        <property type="protein sequence ID" value="ENSPNAP00000006958.2"/>
        <property type="gene ID" value="ENSPNAG00000012901.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158661"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005892">
        <property type="term" value="C:acetylcholine-gated channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000026">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000031">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000012">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="23">
        <location>
            <begin position="14"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="24">
        <location>
            <begin position="229"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006958.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="478" mass="55440" checksum="718508693A1CACDB" modified="2024-11-27" version="1">VKWKNIPEQCSEAERKLLQDLFQNYNLKVRPARTWNETVMVRVGMTLVQLISLVGFPHTHTHTHRTAWSDYRLSWNPKDYDNIDVIRIPPTKVWRPDIYLINNNDGQFDVALYVNVLVTNDGTVSWLPPAIYRSSCSIEVAYFPFDWQNCTMVFRSYTYDASEVDLQYGLDEDGNELHEIVIDKNAFTENGEWQICHKPTRKNIQEDMYEDITFYLIIERKPLFYIINIIVPCILISILAIFVFYLPPGAGEKMTLSISVLIALTVFMLLLADKVPETSLGIPIIVNYVMFTMILVTFSVILSVVVLNLHHRTPSTHYMPTWVRKVFINFLPPYLGMFRPHPEEPVDEEPNEDQPQGHTDRTLKPGGEYFIRKINPELVMPWRGSRPDTTVQLQRFFDSDSYCLILPPDLKSAIAAITYMAEQLKKQDVNDTITDDWQYIAIVVDRLFLWLFVIITTLGTLAMFLDASFNHTPDNPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5N8</accession>
    <name>A0A3B4C5N8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adipocyte plasma membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">APMAP</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5N8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006201"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003979.2">
        <property type="protein sequence ID" value="ENSPNAP00000006201.1"/>
        <property type="gene ID" value="ENSPNAG00000002173.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423292"/>
    <dbReference type="CTD" id="57136"/>
    <dbReference type="GeneTree" id="ENSGT00440000039984"/>
    <dbReference type="OMA" id="YRITRYW"/>
    <dbReference type="OrthoDB" id="5307922at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004064">
        <property type="term" value="F:arylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="adipocyte plasma membrane-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF130">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="10">
        <location>
            <begin position="200"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="415" mass="46628" checksum="15A40EA7E1516B48" modified="2018-12-05" version="1">MNEPEGLRFRRINRPQIITDETHEPQYKGTSTYSGKVFRVTLLTLGGFLLFPLLVVVFLLESPIQPEAFSLNEPPFMTGCFEPNLKLRQAERLFEDQLIGPESIANIGDVLYTGTADGKIVKIDGRKMSVLATLGKPPCGSREQEHVCGRPLGIRVGSNGTLFVADAYLGLFEVNPVTGESTLLVSTRKMVGGRRLSFINDMDVTQDGKKVYFTDSSSRWQRRDYLSLIMEATADGRVLEYDTETKEVTVMMENLRFPNGIQLFPDEESVLVAETTMARIRRVHVAGLNKGGMDTFIDNLPGFPDNIRRSSSGGYWVVMSAVRPNPGFSMLDFLSQRPWIKKLLFKLFSQDMMMKFVPRYSLVVELQDGGTCVRSFHDPHGMVAAHISEAHEYNGHLYLGSFRSPYLCKLDLSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CA03</accession>
    <name>A0A3B4CA03_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MEPCE</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CA03"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007227"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002168.2">
        <property type="protein sequence ID" value="ENSPNAP00000007244.2"/>
        <property type="gene ID" value="ENSPNAG00000013102.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153993"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040031">
        <property type="term" value="P:snRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010675">
        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF0">
        <property type="entry name" value="7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="8">
        <location>
            <begin position="411"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="76"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="481"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="84"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="504"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007244.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="709" mass="78620" checksum="B40CC1305EBDA6E4" modified="2024-11-27" version="1">MIEMSLEKETVLAGDGVGGVLSPPQNLLGRTKTPSPPTQVPIGIGAPFMANMVLAELPPSEVEGGVSETLRVRGDGKPLQLKNGLQSQAGPQQQQQKLSKRRYSMNAGFKHPGFSKRRRRANSDCDPVLPTNFLLGGNIFDPLNLNSLLDEEVNKALNAETPKSSPLPAKSREPVEILIPKDITDPLNLSGRGGDAHGGVLVSPMKSGGRRRHRNRHHGGPNGGQLDPADSDRSKGDEGGRQLFPVQHAEAGQEGAVPEESREGVIPLDLEPVEESPRPYELNTSINCRDEVVPPILPRRRSNTSTNSAPQAQTGSSNSTSSTHPAKHRKRRRTISRSERLSITPTPTVKQSSVDRVRSQTFHTPVVAGAAGGHRTGSRQAAQNKRKPQRKFQYGTYSRYYGYQTPGLNRDPRLAVFRPEWFRGRKVLDVGCNVGHVTLAIAKHWSPAHILGLDIDGRLVHAARQNLRHFLSELQRQEVQRGNSAWGELSRPRRKDDEPEEEGTERLGETKEKREEAEARGGPPGGRARELVPLMKLKLELLRPFPVSFRLCRGPIAAPPLQPHTPGVFPNNVSFMKGNYVPESEAAVMSQRAEYDAILCLGVTKWVHLNWGDLGLQWFFKRAYRHLTPGGVLILEPQPWSSYSKRKRLTETTFRNYNCIRLRPDQFSTYLTSEVGFTSYELISTPNSCPKGLQRPIYLFHKGPASCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAZ1</accession>
    <name>A0A3B4CAZ1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Adhesion G protein-coupled receptor G2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAZ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008768"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014912.2">
        <property type="protein sequence ID" value="ENSPNAP00000008768.2"/>
        <property type="gene ID" value="ENSPNAG00000003096.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164851"/>
    <dbReference type="OMA" id="GWPMIVV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000043">
        <property type="entry name" value="adhesion G-protein coupled receptor G2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF264">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="169"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="199"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="298"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="8">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
        <location>
            <begin position="112"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008768.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="472" mass="52167" checksum="F35C38CD7F173AEF" modified="2024-11-27" version="2">MEFSQATARDLPPQGSIVLPASLTGNLNPQQQQLVSRVQFNFYQKSTLFQVCSTAPLPRCFCGSGGWSSRGCTVLNSTVNETLCGCNHLTSFGVLLDLSRQGITNRLQATILTYITYIGCGISAIFLSITLLTYLAFEKLRKDMPSKILIQLCLALLLLNLVYLLDSWLALYPNAVGLCISTAFFLHYFLLASFTWMALEAVHMYFALVKVFNTYISRFMLKLALAGWGIPLVVVIIVIAIDKDNYGLVSYGKFEDGSTDDFCWLKNNIAFYVAVVAYFCVVFLLNLSMFIVVMVQLCRIKRQNPHNVQHRSGLQDLRSVAGLTVLLGLTWGFAFFAWGPVNLAFMYLFAIFNTLQGFFIFVFHCAVKENVRRQWRIYLCCGKFRLAENSDWSHTATQKTKKSSVNRVGLASVKSSNSNNSSSSATFLVSNGSGSGSGSGSDGSLGISECRPLRATSSVYSLDCHCSTAHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CCV3</accession>
    <name>A0A3B4CCV3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">KCND3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CCV3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008284"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000398.2">
        <property type="protein sequence ID" value="ENSPNAP00000008284.1"/>
        <property type="gene ID" value="ENSPNAG00000013895.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155343"/>
    <dbReference type="OMA" id="PRTECIS"/>
    <dbReference type="OrthoDB" id="433309at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005250">
        <property type="term" value="F:A-type (transient outward) potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097623">
        <property type="term" value="P:potassium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18419">
        <property type="entry name" value="BTB_POZ_KCND3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000111">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000016">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000004">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003975">
        <property type="entry name" value="K_chnl_volt-dep_Kv4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004056">
        <property type="entry name" value="K_chnl_volt-dep_Kv4.3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024587">
        <property type="entry name" value="K_chnl_volt-dep_Kv4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021645">
        <property type="entry name" value="Shal-type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF182">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY D MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11879">
        <property type="entry name" value="DUF3399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11601">
        <property type="entry name" value="Shal-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01518">
        <property type="entry name" value="KV43CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01497">
        <property type="entry name" value="SHALCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017250782" description="BTB domain-containing protein" evidence="21">
        <location>
            <begin position="17"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="22">
        <location>
            <begin position="38"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="600"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="568"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="640" mass="72134" checksum="1DB8898F6DD78FE8" modified="2018-12-05" version="1">MATGVAAWLPFARAAAIGWMPVANCPMPPAPREHSKKQDELIILNVSGRRFQTWRTTLDRYPDTLLGSTEKEFFYNEETKEYFFDRDPDAFRSVLNFYRTGKLHYPRYECISAFDEELAFFGIIPEIIGDCCYEEYKDRKRENAERLMDDQEENKDSKLPNMNFRETMWRAFENPHTSTMALVFYYVTGFFIAVSVITNVVETVPCGSTPNQKDVPCGERYTVAFFCMDTACVMIFTVEYLLRLFAAPSRYRFMRSVMSIIDVVAILPYYIGLVMTNNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGSSSSKFTSIPASFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRRAQKVQKARLARIRIAKKGSSNAYLQSKRNGLLNELLDLTGSEEEEQHMNKTSSLLESQHHHLLHCLEKTTAHEFVDEQLFEQNCIEGAQFSRSPSLSSQEGFTGTCCTHRSRKRIPLPNASLSTTHTHTHTQGLQELSAIHIQCGDQPSLSTSSRSSLNMKSSERSRINCRGQQITTAVISLPSPTNFPPDGDGHSPANPIPAPPTINTTTSSNIVKVSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJM5</accession>
    <name>A0A3B4CJM5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Nuclear factor of activated T-cells 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">T-cell transcription factor NFAT5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer when bound to DNA, completely encircles its DNA target. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm. Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017554929.2">
        <property type="nucleotide sequence ID" value="XM_017699440.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000010719"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017472.2">
        <property type="protein sequence ID" value="ENSPNAP00000010719.2"/>
        <property type="gene ID" value="ENSPNAG00000016335.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428435"/>
    <dbReference type="CTD" id="334390"/>
    <dbReference type="GeneTree" id="ENSGT00940000155213"/>
    <dbReference type="OMA" id="LPMGQQS"/>
    <dbReference type="OrthoDB" id="5346094at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07882">
        <property type="entry name" value="RHD-n_TonEBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000174">
        <property type="entry name" value="Nuclear factor of activated T-cells 5, tonicity-responsive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000002">
        <property type="entry name" value="Nuclear factor of activated T-cells 5, tonicity-responsive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015646">
        <property type="entry name" value="NFAT5_RHD_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF10">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RHD" evidence="23">
        <location>
            <begin position="269"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="36"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="116"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="191"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="702"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="927"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="990"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1105"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1195"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1233"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="796"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="885"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="704"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="944"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="962"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="990"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1008"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="1112"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1233"/>
            <end position="1246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010719.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1447" mass="156476" checksum="3930E77F3B20E5FF" modified="2024-11-27" version="2">MPSDFISLLSTDIDLNSPKSLYSKESVYDLLPKELQLPPTTQDKQTMSQKSGGEAGPLPSAALASDAATASSSPGGHSSALPASSSMHSTASAPEQSSRLHSCLPAEVLGAEPSSASAISGVAGGDGVGVPAHQNQMTPSKRRTVLNISPPPEDLLDDSRMSCQDELPLNPDSEQSSSIWMDDSVSNFSMLSNSSYNDNTEVPRKSRKRTPRQRPGPKPVARDEATMDVFDADSAKAPHFVLSQLGSEAKGNLKGSTLEVAGSQKGGVLSMQYPQKSEGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVNEPVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDVEGTTVIEVNLEPSNSMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVNIPRPDGSVLTLQTLSSPILCTQPAGVPEILKKSLHSCPVIGGEEIFIIGKNFLKGTKVIFQENATDDSSWQAEAEIDMELFHQNHLVVKVPPYHDLTVTSPVSVGISVVTNAGRTHELQPFTYTPQQEKADISVKSELPATSKPCTFEDQITAMQSGTSSLNSTLMAPMISIVKREEVTPMEVSTNPTACNIFKPAEVLNSNQQILEITTVLPTSSKSFPSPVPLQPVDSQQSAPPIFTAPEPLTTIQKQDISPTTSFQVSLSEDTTVLQPVPPQVPQQFLRETPETLSPEDNSPDGTGMVVVSMESRPPPSQQPQVQNLLPQDEVAQLERAVRELQEQQQLNSVLYGPTPSAESLQQHVQENMNSLRLGGNENTLTNQQQQQQEQNILENLQQQHQKALVDLQHQQTVLESLQQQQKVLENLQHQALGSLQQQQSHQKVLENMQHQKVLEDIQQQNSLVNMQHQKVLNTLQQQQQHQKNLEKLQQEQQHQEVLENLQKHLQSELPQIPMQCTPSFSGVPPDQISEHQSTTQTDGTMSQPSDGFLQNSSQQPQQQALFQQAGGILTIQTSSFLQQSPSQSSPPQQLFQSHNPMTETQDPQTALFGSSKASQVQPALFPNTLTMLTSTNLSSEQQAPSTTLFISQGVLPSQITADSTQNQQQQQITFLTPMPPSASAQTVSLFQGQAQLSTPMEQQQSPQPQQIPTPQQASLFQSMSTISQSQAQQPSQTGLLFCATPINPQDLPQTLLFSGQNQGPIGGEQLPQNIFQDQQPMQVVPSSANVAADTSTNQSTVPSQSNQSPQILFPQASVVNVAQQDSSEPMSFQDESSVVDDSATSMPSTRPGLFQDQQPMQVVPSASNVASVSSTDQASVNIFLPQAAISALQSGVGTQELPQAATAAAIFSGQGGVAIGGLQSSTPTSSQPTPDPLFQTALGNTLSQPGQPGQPGLFLFEIPNECGQLLNPSGPALADRLVSMGHPSSDHNQSQLQTSAQIHTLLDQSSNLNEKIDELLERFQEQGKTLSRNFLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPN3</accession>
    <name>A0A3B4CPN3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ras homolog family member A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548933.1">
        <property type="nucleotide sequence ID" value="XM_017693444.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPN3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013315"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036872.2">
        <property type="protein sequence ID" value="ENSPNAP00000013315.1"/>
        <property type="gene ID" value="ENSPNAG00000019146.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425062"/>
    <dbReference type="CTD" id="100006041"/>
    <dbReference type="GeneTree" id="ENSGT00950000182945"/>
    <dbReference type="OMA" id="EVNHYIP"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048840">
        <property type="term" value="P:otolith development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036342">
        <property type="term" value="P:post-anal tail morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000095">
        <property type="entry name" value="Rho-related GTP-binding protein RhoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21843" checksum="4E4117030909E8AC" modified="2018-12-05" version="1">MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDSKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKAEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKNSKCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CU92</accession>
    <name>A0A3B4CU92_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017577808.1">
        <property type="nucleotide sequence ID" value="XM_017722319.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017577809.1">
        <property type="nucleotide sequence ID" value="XM_017722320.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037402538.1">
        <property type="nucleotide sequence ID" value="XM_037546641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CU92"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014094"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037193.2">
        <property type="protein sequence ID" value="ENSPNAP00000014094.1"/>
        <property type="gene ID" value="ENSPNAG00000020025.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442332"/>
    <dbReference type="CTD" id="566270"/>
    <dbReference type="GeneTree" id="ENSGT00940000155893"/>
    <dbReference type="OMA" id="GFVMYFY"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000010">
        <property type="entry name" value="probable cationic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF17">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="569"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="595"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="655"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="7">
        <location>
            <begin position="627"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="715"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="773"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="783" mass="84687" checksum="1BF8A958E96B7FF9" modified="2018-12-05" version="1">MSRVLAKLDPRRVQWSAFSSRILRTKPVESMLDSSASTAGTGPHGTRLARVLTTVDLVSLGVGSCVGTGMYVVSGLVAKEMAGPGVIVSFIIAAVASILSGVCYAEFGVRVPKTTGSAYTYSYVTVGEFVAFFIGWNLILEYLIGTAAGASALSSMFDSLANHSISSFMISHVGTLNGLGKGEQSYPDILALIIAILVTVIVALGVKNSVGFNNVLNVINLMVWVFMMIAGLFFVSGANWDEGRFLPFGWSGVMQGAATCFYAFIGFDIIATTGEEAKSPNTSIPYAITASLVTCLTAYVSVSVILTLMVPYTEIDADAPLMEMFSLHGFQAAKYIVAIGSIAGLTVSLLGSLFPMPRVIYAMAGDGLLFRFLAHVSAYTETPVVACIVSGFLAALLALLVSLRDLIEMMSIGTLLAYTLVSVCVLLLRYQPESDIHGFVNFLSEENAKRKGEGVLAECEKDACSPVSEGEEYGGPPTNTCGAKNLPSLGDNEMLIGKSDKSSYTATHPNYGTVDMSSGIESDETESVYLQKLKKLMGPRYYTMRIRLGLPSKMDRPTPSTGRTVTRCVLLLFILIFGFCSLIIFGASCIAERRWWAIVLLVLLVLVITLLVFIIIQQPENPKRLPYMAPCVPFVPASAMLVNVYLMLKLSSITWIRFAVWCFVGLLIYFGYGMWNSTLEITALEEEVHASTYQRYDMGVDDNFAVDDDLYPSGGGWRPQSQGAKHQSPRENRDPKEAPTDGLDSLENHRTSSSHSRAKSKAGKSVPGFEALVVDDDLDDPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWK1</accession>
    <name>A0A3B4CWK1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AP1G2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572547.1">
        <property type="nucleotide sequence ID" value="XM_017717058.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037387468.1">
        <property type="nucleotide sequence ID" value="XM_037531571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWK1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015718"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037841.2">
        <property type="protein sequence ID" value="ENSPNAP00000015718.1"/>
        <property type="gene ID" value="ENSPNAG00000021773.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438930"/>
    <dbReference type="CTD" id="8906"/>
    <dbReference type="GeneTree" id="ENSGT00950000182838"/>
    <dbReference type="OMA" id="REPNTKK"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003401">
        <property type="term" value="P:axis elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001944">
        <property type="term" value="P:vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000030">
        <property type="entry name" value="AP-1 complex subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017107">
        <property type="entry name" value="AP1_complex_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037094">
        <property type="entry name" value="AP1_complex_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GAE" evidence="11">
        <location>
            <begin position="671"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="789" mass="86743" checksum="44D33E23D8A9DA8B" modified="2018-12-05" version="1">MAPSVCLQEMIRVIRSARTQGEERGVIQRECAAIRAQFRQADSGGRSHNLAKLLYVHMLGYPAHFGQMECVRMIASPRYSEKRIGYLGAMMLLDEKQDASLLITNSIKNDLSHSSQYIQSLALCTLACMGSAEMCRDLAPEIERLLKASNSYIKKKAALCAVHIVRKIPELGELFTPSAHSLLSDKNHGVLHGAVVLITELCERNPGNLDEFRKAVPELVQIMKGLVTSGYSPEHNVAGISDPFLQVRVLRLLRILGRNNDEASDAMNDLLAQVATNTDSSKTAGSAVLYETVLTIMDIKSESGLRVLAVNILGRFLLNNDRNIRYISMTSLQKIVQTDHTAVQRHRGTIVDCLKDQDASVKRRALELSLALVSAANIRSMMKELLRFLSTCPPELRSHATSGIFNAAERYAPSQRWHIDTILHVLITAGGDVRDETVPNLIQLISVASELHCYTVHKLYRALISDISQQSLVQVACWCIGEYGDLLLKGDCDEIEPMQVTEDDVLDVLETILQSHMSSPTSRGFALTAIMKLSTRITNNIDRIRSVVSIYGSCIDVELQQRAVEYNALFKKYDHMRAAVLERMPIMDKNSPGHANGDPSGEITKDLIQTTPIQGLLIQEPASQVCDLLDLLGETNTSSQANQNQVNTTSNSTSSSVQENLLDLLGGLEPTPAPNVTVYEKGGVNLKLQCDNQTETAITVTFTASNSTQSDITSFTLQAAVPKSVQLQMKAPSGDVIPAQGRGTVTQTILLNNPNKASLKMRVRLSYTSQGTLCQDMVQIDSFPSPAWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CXH0</accession>
    <name>A0A3B4CXH0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CXH0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016797"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038266.2">
        <property type="protein sequence ID" value="ENSPNAP00000016797.1"/>
        <property type="gene ID" value="ENSPNAG00000022963.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286900"/>
    <dbReference type="OMA" id="MQEYKDH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057667">
        <property type="entry name" value="HTH_SB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25787">
        <property type="entry name" value="HTH_SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sleeping Beauty transposase HTH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="69"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="192"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="41321" checksum="7789C81840BC1B42" modified="2018-12-05" version="1">MGRSQELSDFERGAVIGCHLCSKSSREISSLLNIPQSTVSCIITKWKRLGTTATQPRSGRPRKMTEQGQRMLKHIVRRGHQLSAESIATDLQTSCGLQISTSTVRRELHEMGFHGGAAASKPYTPKRIAERRMQWCKAHRHWTLEQWRRVLWSDNSRFSVWQSDGRVWVCRLPEERYLSDCIVPSVKFGGGGIMVWGCFSQSGLGPLVPVKGTLNASAYQEILDNFVLPTLWEQFGDGPFLFQHDCAPVHKARSIKTWMSEFGVEELDWPAQSPDLNPMEHLWDELEQRLRDRLFRPTSVSDLINALQEEWLKIPINTLLNIVESLPRRVEAVIIAKGGPTSYACEGKQANTHTGSSQEKNNITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CY36</accession>
    <name>A0A3B4CY36_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PTB domain-containing engulfment adapter protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cell death protein 6 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">PTB domain adapter protein CED-6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ced-6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566106.1">
        <property type="nucleotide sequence ID" value="XM_017710617.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017566107.1">
        <property type="nucleotide sequence ID" value="XM_017710618.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017566108.1">
        <property type="nucleotide sequence ID" value="XM_017710619.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017566109.1">
        <property type="nucleotide sequence ID" value="XM_017710620.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017566110.1">
        <property type="nucleotide sequence ID" value="XM_017710621.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CY36"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016243"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024676.2">
        <property type="protein sequence ID" value="ENSPNAP00000016243.1"/>
        <property type="gene ID" value="ENSPNAG00000022465.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435068"/>
    <dbReference type="CTD" id="368404"/>
    <dbReference type="GeneTree" id="ENSGT00940000156186"/>
    <dbReference type="OrthoDB" id="10057585at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01273">
        <property type="entry name" value="PTB_CED-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000118">
        <property type="entry name" value="GULP PTB domain containing engulfment adaptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF70">
        <property type="entry name" value="PTB DOMAIN-CONTAINING ENGULFMENT ADAPTER PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="8">
        <location>
            <begin position="25"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="374" mass="41728" checksum="2DD1BE5049364C1E" modified="2018-12-05" version="1">MSRAFNRKKDKSWMHTPEALAKHFIAYNAKFLGNTEVDQPKGTEVVRDAVRKLKFQRHIKKSEGQKIPKVELQISIYGVKILDPKTKDVQHNCQLHRISFCADDKTDKRIFTFICKDSESNKHLCYVFDSEKCAEEITLTIGQAFDLAYKKFLESGGKDVETRKQIGSLQKRIQELETENSELKKHLQTLEEQLLIAQVPPLLHINSTNKDFMLQRPSSLFWCHNLTSFSCLEISSVTLTPMSSPDSNMSAGVLTPPPTKPALPKPLTEAGIPRPHGGGNSKVPTDIFDMVPFSPVAPLVPLPASNASPPPPPARPAEIRKDLFGAEPFDPFTCGAADFPPDIQSRLDEMQEGFKMGLTVEGTVFSLDPLDGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D083</accession>
    <name>A0A3B4D083_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription factor HIVEP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Kappa-B and V(D)J recombination signal sequences-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Kappa-binding protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Zinc finger protein ZAS3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with TRAF1 and TRAF2 as well as with JUN. Forms a multimeric complex with RUNX2 and E3 ubiquitin ligase WWP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017579563.2">
        <property type="nucleotide sequence ID" value="XM_017724074.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017579564.2">
        <property type="nucleotide sequence ID" value="XM_017724075.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392757.1">
        <property type="nucleotide sequence ID" value="XM_037536860.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000016249"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038059.2">
        <property type="protein sequence ID" value="ENSPNAP00000016249.2"/>
        <property type="gene ID" value="ENSPNAG00000022386.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443422"/>
    <dbReference type="CTD" id="568037"/>
    <dbReference type="GeneTree" id="ENSGT00940000157218"/>
    <dbReference type="OMA" id="EDRGPPN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000033">
        <property type="entry name" value="Immunodeficiency virus type I enhancer binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000083">
        <property type="entry name" value="Immunodeficiency virus type I enhancer binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051969">
        <property type="entry name" value="Zinc-finger_DNA-bd_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944">
        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR HIVEP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1441"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="20">
        <location>
            <begin position="1469"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="189"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="390"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="664"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1141"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1373"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1507"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1799"/>
            <end position="1964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="472"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="690"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="764"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="780"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1195"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1384"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="1507"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1625"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="1690"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1701"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1724"/>
            <end position="1737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1806"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1835"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1865"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1901"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1953"/>
            <end position="1964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016249.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1964" mass="217656" checksum="2A7F8C62BA208AB7" modified="2024-11-27" version="2">MEAEHSRPADGERSGGQQQPLSESSLRSRLALPQEQATSQPIHGSHGRHQHRQPKRSDMLQRQQQQQSQVRESSWQLPDVPGASRGRYSSSRLESSTQASHAQRTPSQPSQVVQEGTPSRRERRPQKPGKYVCTYCGRPCAKPSVLQKHIRSHTGERPYPCVPCGFSFKTKSNLYKHRKSHAHRIKAGLASSREEHSFSGAESGTVGDEQEEATEAESTDSEDETGQRRPSTSQDRPALRKSSKVEMSFSEEGPRTEDSQAVKQRLAMRLSERKRVPIISPDETPSSSLGPGSKGSTESGYFSSYGSAELSQVSPPNANAKSYAEIILGKYGRLGQQQRVPHQHLQSSSSSTSLGQEEKSIPFTVPKTQVIEHITKLITINEAVVDTSEIDSVKPRRSSLSRRSSIESPKFSSPKDPCVFDPKGDVPGPSGSSGQIILSGFATERQHSQLVEDTLAGQCSSAVLLRSQSVPTSGGQDDTTSTSSHSFRLSHSFDEQQAAAAEMRVGHHHRMLRRQPAIEMPIGADLVAEAGPSTSTLVESGWKCEICGTLFKKQDCYAAHRVACMHKPSHDQSEKEGSAVREDRPQMMHYKFKAMAMAVRKRRKEESLEEDPPSPGPAAVSFNIQSTTSMIGTQGDFKGFSGPPSQTELEKMGPFKEISVIQHTSSFEKQESMSIESQESELEHEQSQEPKQTSTSRLVRQPNIQVPEILVTEEPDTEMVSPPASTCVPKELEKEEFQWPQRSQSLAQLPAEKLPPKKKRLRLAEAAQSSGESSFESVSLPRSPSQESNVSHTSSRSASFDESGKTDSEMHIGTWSSQGSHMLTVPGPYQHHHSHREMRRSASEQAPASPPHPAQIVETRSKSFDYGSLSPKPTSASWKERRKCLLVKHATLGKPDQEEPLSSLQSNPIVCRSYPPIVCQSYPDHPGLSIDEHRTSNIFYPLTSPDTFSPRQLTRFLPVTTTTSEVLSTHMFQQALIHTVPSPLHPRQFHLPEHLVLPVQPFPTLLPLQYSSSGLTIRVPSGPPVRESRTSPHLVHSPSSSYHQLVTHHQPRPIIATCLEQLTPVVSLVVPVRLQTQISNYGSAMYTTISQILATTRSQQPICCTAMVIMGKLEGSKLQRSYLRLPTPDFKTGIPMSLPLEFGSESASDESSGPLVAGGSKRMLSPAGSLELTLEAQRQQKRVKEEDEEEEEEERREKDTKHSEKAQEESQVPDKEALTVGIEWRKQEREVKKTEELQASVMNKQEVVQVETEPFKKEEGEEESHRCVSVFEINERSRDPLYPSLHTSTSVSWCYLNYTKPNPTAHRSPQTSVYSSWSVSMYNPNLPGLTTKMVLSLLCSKQTYSSEMYTMATAPPVTTEKLVPASNKKPMVSEVCAKPPSTSDKVKEEPVSKRVEKEEKSAEESATTSQQSEAPRICIFEGGYKSNEEYVYVRGRGRGKYVCGECGIRCKKPSMLKKHIRTHTDIRPYVCKHCNFAFKTKGNLTKHMKSKAHGKKCQEMGVSQSIDELEAEEAGTSEEQVYESEDQEEHQFSDVEDSEDEDDDNEEDEEEVSSHDEPPSSCSTDTRHSDSAQADLPTPTAPERENHPSPCRAWSSGRAASPGSKRALFSRKGWELSPRAFSPVSEGSPLRSLSPRLELSSPSRHLSSSPERGPSPIRASPVRPLSPLRPLSPARYRSSRANVSPSPLRAQHRPQHRPHSSPGQFHWDSSTQARESQQERPGTPGTSQTPQRLSPSHPSFLPPALRLSHTDPSLHLPVTPQTLDRMFSHLPLHSQDQARMPYHMIPIGGIQMVQLRPRTRPKLERSLSSTPSPTSPNEDAPFFLGRRDAPWSLHPETSQQSTLAFSETQSMERGTGPSGLKGPVSRSTSAKSSLQYGNGEQLDKDRKQYGSSRPSKAHTIVPQTSQEVGQDNSKGTERTPGFGEKEERASSHRTKQLSELESEESAGDKSVCPGQSQESNPDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3M6</accession>
    <name>A0A3B4D3M6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nonsense-mediated mRNA decay factor SMG9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SMG9</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMG9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557547.1">
        <property type="nucleotide sequence ID" value="XM_017702058.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3M6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018952"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039232.2">
        <property type="protein sequence ID" value="ENSPNAP00000018952.1"/>
        <property type="gene ID" value="ENSPNAG00000025511.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429962"/>
    <dbReference type="CTD" id="56006"/>
    <dbReference type="GeneTree" id="ENSGT00390000003568"/>
    <dbReference type="OMA" id="SEYYPHL"/>
    <dbReference type="OrthoDB" id="79514at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001272">
        <property type="entry name" value="SMG9 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019354">
        <property type="entry name" value="SMG8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039177">
        <property type="entry name" value="SMG9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14270">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR SMG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14270:SF0">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY FACTOR SMG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10220">
        <property type="entry name" value="Smg8_Smg9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="504" mass="56097" checksum="B6B9F98BA8F0B254" modified="2018-12-05" version="1">MSESGHSQPGMYGQGRRRRRRRDREPGPPGQNLSGPSRDRDFGPRERRDGSEDPPGPLLQKTPIILAKPPGERSKQSAPVSGTSLEKPIMLMKPREEGGKSAAASDTASPASGPGVAKLEREGQRPTQPVYQIQNRGMGSAASAGAVDPVIGQTKLLPPEKMKHSIKLVDEQMNWCDSAMEYLRDQTDMLVVGVIGLQGTGKSTIMSLLSANTPEEDQRAYVFRAQTQEIKERGGNQSSGIDFYITQERVIFLDTQPILSPSILDHLINNDRKLPPEYNLPHTYIEMQSLQIAAFLFTVCHVVIVVQDWFTDINLYRFLQTAEMLKPSTPSASHDSTGSSGQDDGSEYYPHLIFLQNKARREEFCPRNLKNMHMVVDKLMAHSHLKYKGTLSMLDCNIFPGLERDYLGTEVNLFLLPVLENDGDEALTRAGSGSMPLFSLLPGYRGHPSFSSLVSKLRSQVLAMSRNQLSHTILTEKNWFHYAARIWDGVKKSSALSEYSRLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3X1</accession>
    <name>A0A3B4D3X1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Periphilin-1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PPHLN1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017573299.1">
        <property type="nucleotide sequence ID" value="XM_017717810.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3X1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017584"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026415.2">
        <property type="protein sequence ID" value="ENSPNAP00000017584.1"/>
        <property type="gene ID" value="ENSPNAG00000023896.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439410"/>
    <dbReference type="CTD" id="51535"/>
    <dbReference type="GeneTree" id="ENSGT00390000016228"/>
    <dbReference type="OMA" id="HVEYRDY"/>
    <dbReference type="OrthoDB" id="8933311at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097355">
        <property type="term" value="P:protein localization to heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22896">
        <property type="entry name" value="periphilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057603">
        <property type="entry name" value="Periphilin-1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028851">
        <property type="entry name" value="Pphln1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15836">
        <property type="entry name" value="PERIPHILIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15836:SF4">
        <property type="entry name" value="PERIPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25234">
        <property type="entry name" value="Periphilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Periphilin-1 C-terminal" evidence="2">
        <location>
            <begin position="274"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="361" mass="40734" checksum="42DF4E9B89FD1E6A" modified="2018-12-05" version="1">MAFRRDRNIRDMYEERFHPERAAPYQRGVGGERRPSFGRPDDDYGRSFDGPRFYPNGAPRNYHGEDQRGYHGDSHHSFSNEPRGGPPGRRDDYPYYRGPREEPHSGRQVDFRGSSRVGPPPSARVQGSYPAPRSLSALGPDQGDDTLIQAIMNLDRGEEREGLRRKAPFPPVRDRSPVRRDVPPSPHSRSGSSISSRSYSPDRGKTHQYQPPQGKRFEEPYGPSRSADRERAPGPSVSASRDGSPHSSVSASKEEIPVTEGPTDEPSAATDEGSTVMDDFQERRSQAIAAKAREIEKVYRQDCETFGMVVKMLVAKEPSLEKHLQTPLKENLSEIRERCLEDLRHFISELDEVVRQPEPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4G0</accession>
    <name>A0A3B4D4G0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Oxaloacetate tautomerase FAHD1, mitochondrial</fullName>
            <ecNumber evidence="5">3.7.1.5</ecNumber>
            <ecNumber evidence="2">4.1.1.112</ecNumber>
            <ecNumber evidence="9">5.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Acylpyruvase FAHD1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Fumarylacetoacetate hydrolase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Oxaloacetate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">FAHD1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-fumarylpyruvate + H2O = fumarate + pyruvate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26168"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16854"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3-acylpyruvate + H2O = a carboxylate + pyruvate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19009"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57278"/>
            <dbReference type="EC" id="3.7.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>acetylpyruvate + H2O = acetate + pyruvate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16097"/>
            <dbReference type="ChEBI" id="CHEBI:15360"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxaloacetate + H(+) = pyruvate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15641"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="EC" id="4.1.1.112"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>oxaloacetate = enol-oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:16021"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17479"/>
            <dbReference type="EC" id="5.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:16023"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.5" evidence="5"/>
    <dbReference type="EC" id="4.1.1.112" evidence="2"/>
    <dbReference type="EC" id="5.3.2.2" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017561356.2">
        <property type="nucleotide sequence ID" value="XM_017705867.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D4G0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019237"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028904.2">
        <property type="protein sequence ID" value="ENSPNAP00000019237.2"/>
        <property type="gene ID" value="ENSPNAG00000025781.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432194"/>
    <dbReference type="GeneTree" id="ENSGT00940000160452"/>
    <dbReference type="OMA" id="NCRKVIC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047621">
        <property type="term" value="F:acylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000003">
        <property type="entry name" value="Fumarylacetoacetate hydrolase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007967">
        <property type="entry name" value="PRK10691.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="15">
        <location>
            <begin position="15"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019237.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="219" mass="24260" checksum="3E258868D7415DFE" modified="2024-11-27" version="2">MTARNIARFWEWGRKIICVGRNYAEHAKELRNAVPSEPVLFLKPPSAYVKEGSPILVPRYCSSLHHEVELGVVIGKAGTAIPQSSAMEHIAGYALCLDMTARDIQDECKSKGLPWTLAKAFDTSCPVSEFIPKERLPDPANVTLWLKVNDVERQNGSTSQMIFSIPYLISYISEVISLEEGDLILTGTPKGVSAVQEHDELQAGIDGIVTMTFQIDKTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4H8</accession>
    <name>A0A3B4D4H8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cell cycle progression 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CCPG1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017560519.1">
        <property type="nucleotide sequence ID" value="XM_017705030.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D4H8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018445"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027633.2">
        <property type="protein sequence ID" value="ENSPNAP00000018445.1"/>
        <property type="gene ID" value="ENSPNAG00000024844.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431702"/>
    <dbReference type="CTD" id="9236"/>
    <dbReference type="GeneTree" id="ENSGT00940000166643"/>
    <dbReference type="OrthoDB" id="9935772at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051990">
        <property type="entry name" value="CCPG1/PBIP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28638">
        <property type="entry name" value="CELL CYCLE PROGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28638:SF2">
        <property type="entry name" value="CELL CYCLE PROGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="158"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="584"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="625"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="319"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="642"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="815" mass="93826" checksum="DFE3B490C4F3F6A8" modified="2018-12-05" version="1">MSESSSDTESSCGWTVISNEGSDIETLGPENGVDYDQEEVDRPPAPDQPASPREADAAAAAGDSSLDGTLIEETVVEAESGQQAGEGAGLEEHVTLCSSSDHSDIITLGDSRETEISAWEEPVAKEDEQPTHEELYLGTSSSSQYAFSAAETIFPAERPVRRDSCSTSTEDEDGGMKVSPAVRRRRLRRSTAGSEPEEQQEVHGEREEGGDGAEDEAEEYREAHIFAPQQRQVSGTLNKCILLALIIAISMGFGHFYGTVQIQERHRMVGKTRVNELSETRELHQCRREKDTVSKDVVESLREDLEDKQDMVMSLTGIMDKITKENQHLRAKEAELQAQREDLFEQFQQAKTEMESRQKHLALENQALKSSLEREEASLSALQDELRHLRAQIRELEEKGAGTDSILSENQRLKDHLLEEKQRVRSFVNQKEALMAEAQVLRKELDKERKVTDRLREELEELSQKDSGADEDPETEELQTRLQELEKKLSFEQQRSDLWERLYVETKEERAKGDRPIKARKPKEGMMGKVKETFDAVKNSTKEFVHHHKEQIKKAKEAVKENLKKFSDSVKSTFRHLKDSALDMFEKSHRPQQKRSHERKDAKMEQQQFKNEGFKEQGNKHMEDTHWQHHRPLHQHPRKSTVDSSSFQADRNTRKSGAQKEQVTMGQRDVPKGCTGVFDCAYQESMSLFNKAMEPIRADEFNQLLHSYLQQEVGHFHHWRELESFINNFFHNGLFIHDQMLFTDFVSGVEDYLEDMDEYQGGDDEVFEDLDDYIYRHFFGDTYLKRYGPSVSSWLYFECLEVERHLYPCEWEGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7J9</accession>
    <name>A0A3B4D7J9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actinodin2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7J9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019485"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029320.2">
        <property type="protein sequence ID" value="ENSPNAP00000019485.2"/>
        <property type="gene ID" value="ENSPNAG00000026088.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000045505"/>
    <dbReference type="OMA" id="LRICNPE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033333">
        <property type="term" value="P:fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019485.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="52807" checksum="42D100CEB81ADDBE" modified="2024-11-27" version="2">MPLQLIPEVLDGAPSVQRTQLSRHRRNVAYYKRLPEFWGWYKYFMDTHNQEGIENLDQLYRTYLQNKHRVEEGRSYNHYLNHLSEIYKACANSDDPDCVAESTSKPTAKIVMPSALKQGVVKVCNPYLDPYCIFVHGPQAPPEEPSPPPVKAPAPIFTPVLPMPLNTPLGYYYAPVLDPFLSAEQKSELLRICNPSDVECLQYHLRAAYGYKPALGPAPSYAHLGCDPSVDPYCMSTLAAKAPSGLYHLYPNCNPAVDPLCVLNVAPAAQTASSEEAPREQYCNPLFDKDCNPLTATKLASLTKPVLEYTPQDEPAPLNLACDPRYDPYCLMGGTAALMKPPPLLPQFQTRSQLGVRGKTKEGYDCFVFYDKDCTPLEAHDFKASAASTQSDCHPYDPNCGKIAPQASTGALAAKTVRDGIIEPHPDCDPEIDFNCRLRRAKTAANSEQCSVAPEYLVPRFEDFLRGYMSHYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8L2</accession>
    <name>A0A3B4D8L2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Androgen-induced 1 (H. sapiens)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">AIG1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>12-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52072"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136312"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:52073"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>12-(9Z-octadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136302"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:52061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>12-hexadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52056"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83677"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:52057"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>12-octadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136330"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:52081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>13-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52076"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:52077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>13-(9Z-octadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136303"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:52065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>13-octadecanoyloxy-octadecanoate + H2O = 13-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136335"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:52085"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>9-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 9-hydroxy-octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52068"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136309"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:52069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>9-(9Z-octadecenoyloxy)-octadecanoate + H2O = 9-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52048"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136282"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:52049"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>9-hexadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52052"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83670"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:52053"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>9-octadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:52097"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556492.1">
        <property type="nucleotide sequence ID" value="XM_017701003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8L2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020697"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031448.2">
        <property type="protein sequence ID" value="ENSPNAP00000020697.1"/>
        <property type="gene ID" value="ENSPNAG00000027624.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429333"/>
    <dbReference type="CTD" id="51390"/>
    <dbReference type="GeneTree" id="ENSGT00940000158696"/>
    <dbReference type="OMA" id="MRTTHHK"/>
    <dbReference type="OrthoDB" id="1898221at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF11">
        <property type="entry name" value="ANDROGEN-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27266" checksum="965AD0AAECBC9E16" modified="2018-12-05" version="1">MALIPSQILRVAILLSYFSIICNYKAIDMPAHQTYGGSWKFLTFIDLVIQAVFFGVCVLTDLSSLLTKGTASMEQERQLRKLISLRDWMMAVLAFPVGVFVVTMFWTLYLYDRDLVYPRLLDNFIPQWLNHGMHTTVLPFIIIEMRTTHHQYPSRLCGLLAVCSFAVGYVLWMCWVHNVTGVWVYPLLEHIGPMARVLFFVCLTGLISLYYVLGEILNSYIWDSSRSAVESNKDKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9H9</accession>
    <name>A0A3B4D9H9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017542793.2">
        <property type="nucleotide sequence ID" value="XM_017687304.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9H9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019761"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029801.2">
        <property type="protein sequence ID" value="ENSPNAP00000019761.2"/>
        <property type="gene ID" value="ENSPNAG00000026436.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414441"/>
    <dbReference type="GeneTree" id="ENSGT00950000183127"/>
    <dbReference type="OMA" id="AMKWAND"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019882">
        <property type="term" value="P:antigen processing and presentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000353">
        <property type="entry name" value="MHC_II_b_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF99">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DRB1 BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00969">
        <property type="entry name" value="MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044005750" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="109"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019761.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="257" mass="29040" checksum="D568B4551BCEA5C1" modified="2024-11-27" version="2">MPPVLHLTAFLWVLSATVHSYYFFYSVECRTLGSPQNIEVLISLVYNKGKVLTYNSTENEVVAYSEYGEKWAKDFNSKTHWLHSEAEKVISDCREYGGQLSPVDRAVRPRVMLRSLRPASGDQPAVLMCSAYDFYPKPIKLTWLRGSEKVTTDVVSTEELTNGDWYYQVHSQLEFTPEPAEDISCMVEHLGFREPALFHWSHSDEASLSESDRDRIAVGAAALLLGGVTALTGLVYYKRKLTGWTSVQVLELGSRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9J7</accession>
    <name>A0A3B4D9J7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017578538.1">
        <property type="nucleotide sequence ID" value="XM_017723049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9J7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029629.2">
        <property type="protein sequence ID" value="ENSPNAP00000019644.1"/>
        <property type="gene ID" value="ENSPNAG00000026294.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442819"/>
    <dbReference type="GeneTree" id="ENSGT01130000278414"/>
    <dbReference type="OMA" id="HRFPCCS"/>
    <dbReference type="OrthoDB" id="9845816at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF882">
        <property type="entry name" value="OLFACTORY RECEPTOR 11A1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="31"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="327" mass="37037" checksum="6074634FEC3BD694" modified="2018-12-05" version="1">MMPNQTQEDALSYVRVRVWASAAAFLILFFFNLLISWTIVREPRLRGHARFVLVLQLLVSASVYFALCFAFHLQLYLGAHLPPASCATLVTALTTSACSILLTITAMALDRYAAICFPLRYGYASRGAWPWLLGAFTWALAAVIPLCLYQKVHTEASKSCGRNTLKGGEVHKILLISACTLLILYSYARILWEGRRLGVLNRRNRAACRTIALHGTQLAVYILPNFVNFVLHVLRNNKSLSDAAKERFAVIAFVFFSLAQCIAPVVYGLRKEELLEQVGHRFPRLNCKLKSLLEWTVRAVHPHRRQQRERRMTSETLLSREISQTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DA02</accession>
    <name>A0A3B4DA02_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peripherin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Retinal degeneration slow protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRPH2/ROM1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551597.1">
        <property type="nucleotide sequence ID" value="XM_017696108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DA02"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019804"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029948.2">
        <property type="protein sequence ID" value="ENSPNAP00000019804.1"/>
        <property type="gene ID" value="ENSPNAG00000026496.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426532"/>
    <dbReference type="CTD" id="58085"/>
    <dbReference type="GeneTree" id="ENSGT00940000157303"/>
    <dbReference type="OMA" id="LCCFLMR"/>
    <dbReference type="OrthoDB" id="9836210at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03162">
        <property type="entry name" value="peripherin_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000002">
        <property type="entry name" value="Retinal outer segment membrane protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000830">
        <property type="entry name" value="Peripherin/rom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018498">
        <property type="entry name" value="Peripherin/rom-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042026">
        <property type="entry name" value="Peripherin_LEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF202">
        <property type="entry name" value="PERIPHERIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00218">
        <property type="entry name" value="PERIPHERNRDS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00930">
        <property type="entry name" value="RDS_ROM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="345" mass="39225" checksum="D14700EC953CE738" modified="2018-12-05" version="1">MALLKVKFDLKKRVKLAQMLWLMYWFSIMAGVLVLSLGLFFKIELRKRSELMDNNESHFVPNLLIGVGLLACGINAFGGKICYDSLDSSKFAKWKTVMKPFLICCVLFNALLFFSALLCFTMRIPLQFTLAEGLKNGMKYYKDTDVPGRCYMKRTLDLMQMEFRCCGNNNYKDWFEIQWVSNRYLDFSSKEVKDRISSNVDGQYLLDGVPFSCCNPSSPRPCIQHQVTNNSAHYSYEHQTEDLNIWKRGCREALVSYYGGLMNTIGVLVLLVTILEVAVMVGLQYVNTSLSTLVNPEDPESESEGWILEKTVKETFTDIMAKMKAMGKGNQVEEGADEQAVATVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAE3</accession>
    <name>A0A3B4DAE3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Profilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the profilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575810.1">
        <property type="nucleotide sequence ID" value="XM_017720321.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DAE3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020418"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030988.2">
        <property type="protein sequence ID" value="ENSPNAP00000020418.2"/>
        <property type="gene ID" value="ENSPNAG00000027254.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441046"/>
    <dbReference type="CTD" id="375189"/>
    <dbReference type="GeneTree" id="ENSGT00390000017067"/>
    <dbReference type="OMA" id="QGQKFML"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF2">
        <property type="entry name" value="PROFILIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00392">
        <property type="entry name" value="PROFILIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020418.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="136" mass="14666" checksum="BF76DB70C28F221B" modified="2024-11-27" version="2">MNPFQNLIVDCLINTKHVESAAVLVAHSGSVAAASPGLRVRPRDAQALTGAFRRLAAVRERGLERGLSGSFSFQEEGFGCVRADRISIYCKRGGRGLLLVRTGLFIIAATYSEDMLPGVCVEAVEALAEYLRQKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DB91</accession>
    <name>A0A3B4DB91_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Focal adhesion kinase 1</fullName>
            <ecNumber evidence="4">2.7.10.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein-tyrosine kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">pp125FAK</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21 28">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_037393171.1">
        <property type="nucleotide sequence ID" value="XM_037537274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DB91"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020713"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031487.2">
        <property type="protein sequence ID" value="ENSPNAP00000020713.2"/>
        <property type="gene ID" value="ENSPNAG00000027658.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436591"/>
    <dbReference type="CTD" id="142986"/>
    <dbReference type="GeneTree" id="ENSGT00940000155113"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007172">
        <property type="term" value="P:signal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13190">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05056">
        <property type="entry name" value="PTKc_FAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.330:FF:000001">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000058">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000021">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000047">
        <property type="entry name" value="focal adhesion kinase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000039">
        <property type="entry name" value="Focal adhesion kinase, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000004">
        <property type="entry name" value="Protein-tyrosine kinase 2-beta isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.540">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041390">
        <property type="entry name" value="FADK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049385">
        <property type="entry name" value="FAK1-like_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041784">
        <property type="entry name" value="FAK1/PYK2_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036137">
        <property type="entry name" value="Focal_adhe_kin_target_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005189">
        <property type="entry name" value="Focal_adhesion_kin_target_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221:SF9">
        <property type="entry name" value="NON-SPECIFIC PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21477">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18038">
        <property type="entry name" value="FERM_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03623">
        <property type="entry name" value="Focal_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68993">
        <property type="entry name" value="FAT domain of focal adhesion kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="FERM" evidence="25">
        <location>
            <begin position="73"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="475"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="736"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="895"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="736"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="782"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="895"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020713.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1108" mass="125012" checksum="DBFCDF51AF9859DA" modified="2024-11-27" version="2">MESSGCSPFPLCWARGACVYSYPPPLPKYDRHLAPTKGMAAAYLDPNLNHALSQGVKPRFSGGMERAAGAMERVLKVFHYFESSSEPCTWASNIRHGDSTDIKGVIQKIVDIHKVRCVTCFGLRLSHLRTGEVHWLHPDMGVSRVRERYEHGHPQDEWRYELRIRYLPKGFLNQFTEDQPTLNYFYHQVRNDYMLEVADQVDQDIALKLGCLEIRRFFREMPGNALDKKSNYELLEKDVGLRRFFPKSLLDSVKAKSLRKSIQQTFKQFANFNDEQSILKFFEILSPIYRFDKECFKCALGSSWVIAVELAIGPEEGISYLTDKGSAPTHLANFTQVQSIQFEEQEKKGMLQLDVAGAAEPLTVTTPSLSTAENMADLIDGYCRLVSGTSLSFIVRIQKEGERALPSIPKAPKASNHEKRLQGVKARAISISDEISGDETDDYAEIIDEEDAYTMPSKYYGLDEARDYEIQRERIELGRCIGEGQFGDVHQGDYVCPESPALSVAIKTCKNSTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILFSYQLSTALAYLESKRFVHRDIAARNVLVSSVDCVKLGDFGLSRYMEDSSYYKASKGKLPIKWMAPESINFRRFTSSSDVWMFGVCMWEILMYGIKPFQGVKNNDVIGRIENGERLAMPPNCPPTLYSLMTKCWAYDPSKRPRFNELKAQLSTILAEEKAQQEERSRMEMRRQVNVSWDSGGSDEAPPKPSRPGYPSPRSSESFYPSPQHMGQHNHYQVGGYPGPHGMPAMPCGIYPPQAAGLGLDPHDGWNHHRAAQDHPMWNPSLEDGGALRAGMGGQSLPPHLMEEQLMLQQQQMEEDQRWLEQEERFMKPDLRNSRGSIDREDCTLQGPPGNQHIYQPVGKTDHVVPPKKPPRPGAPTHPVSAACLSPADSYNDGVKIQPQEISSPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRNLLATVDETIPLLPAHTHREVEMAQKLLNSDLAELINKMKLAQQYVMTSLQQDYKKQMLTAAHALAVDAKNLLDVIDQARLKLLGQARPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBY7</accession>
    <name>A0A3B4DBY7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">60S ribosomal protein L5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567768.1">
        <property type="nucleotide sequence ID" value="XM_017712279.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBY7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020968"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031957.2">
        <property type="protein sequence ID" value="ENSPNAP00000020968.1"/>
        <property type="gene ID" value="ENSPNAG00000005904.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436062"/>
    <dbReference type="CTD" id="326961"/>
    <dbReference type="GeneTree" id="ENSGT00950000183210"/>
    <dbReference type="OMA" id="PHFGLKV"/>
    <dbReference type="OrthoDB" id="1618453at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000002">
        <property type="entry name" value="60S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="10">
        <location>
            <begin position="236"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="251"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="297" mass="34102" checksum="07DE32ECFA481395" modified="2018-12-05" version="1">MGFVKVVKSKAYFKRYQVKYRRRREGKTDYFARKRLVIQDKNKYNTPKYRMIVRFSNRDIVCQIAYAKIEGDVIVCAAYSHELPKYGVSVGLTNYAAAYCTGLLLARRLLNKFSLDKVYEGQVEVTGEEFNVESIDGQPGVFTCYLDAGLTRTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHILGLNISEYMSYLIEEDEDAYKKQFSQYIKNGVTPESVEGMYKKAHAAIRENPVHEKKPQREIKKKRWNRKKLTLSQKKDRVAQKKASFLRAQEAADDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGK7</accession>
    <name>A0A3B4DGK7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Retinoblastoma-like 2 (p130)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RBL2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGK7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034873.2">
        <property type="protein sequence ID" value="ENSPNAP00000022640.2"/>
        <property type="gene ID" value="ENSPNAG00000030051.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183202"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000048">
        <property type="entry name" value="Retinoblastoma-like 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.140:FF:000001">
        <property type="entry name" value="Retinoblastoma-like 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015030">
        <property type="entry name" value="RB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF8">
        <property type="entry name" value="RETINOBLASTOMA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01369">
        <property type="entry name" value="Rb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="12">
        <location>
            <begin position="88"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="13">
        <location>
            <begin position="401"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="796"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein C-terminal" evidence="14">
        <location>
            <begin position="969"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="595"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="747"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="881"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1042"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="604"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="747"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="887"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="908"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="921"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022640.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1088" mass="122341" checksum="81934DAE159E7EE0" modified="2024-11-27" version="2">LTNNPLQSPAPKLLPEKALWETCVKMNEEEDAMQKTRLAFEDLCRALNMDEEASNGAWSSYENISKNYTLEGSELHWLACALYVACRTVVPTVGKGTAEGNYVSLTRILRCSEQSLIEFFRKMKKWQDMANLSKEFRQSTEKLERNFTVTAVIFKKYVPIFKDIFKTPSEEPPRAHRSRKQRRHPCTVAEVFHFCWVLFVHAKGNFPMISDDLVNSYHLLLCALDLVYSNSLLCSNRKDLLNPSFKGLPEDFSNKDYKPSSPLCFIEQLCELHDGLVLEAKGVKEHFWKPFIKRLFERKILKGKEETLTGFLDPVNFGDSLASLNRVYEEHILSSGALDERIFLGEGAKEDIGTPGPCLCEGIENQERPANLLYNSLTSSALKVSTPLTGRKYIQDSGVGTPVSMAMQGVGRLHSLLAGLKHRPSLRLRETLKIKFCSREFQKFTLNYLPLYSYIFLDIALKYFCLAEALYYKTLEGIIDQEKRRLGDSDLSSILEQDMFHRSLVACCLEIVIFSYRPPGEFQQVLQVFDIPPYHFYKVIEVLVRAEDGLFREAVKHLNHVEEQVLESLAWKETSPLWDSIKGAKNHVPSCQEVMPPQYLEDGSGSNPQSPRNNELSANTAGAVKGVFPSTTTLHERYSTPPTGTVCRRLFVDTDSNSDPATSGRVTQAPLVSTIPAGQTVVTMATATVTANNGQTVTIPVQGIANENGGITFIPMPVSVTGQSGTGLQPLTAQALTGTLNTQQLTATTPTSTAHSSPTAGKQSKKSPLQGAPLNRTQRTGSLSLFFRKVYHLASVRLRDLCVKLDISTELRRKIWTCFEYSLVHCTELMMDRHLDQLLMCAIYVMTKVTKEDKSFQNIMKCYRSQPQASSSVYRSVLISRRKRRQSGNSENNNRQTSPSEGGHEQGSGDSSPMSMRSSSTLPIPQPSSAPSTPTHIPGAPPEQEEEEERGDLIRFYNHIYIKQIKHFALRYSSSSPKTPPLCPYPLLRIGSPRRVLLSQNHSIYISPHKSNSPLTPRDKIFYYISSSPSNRLREINSMIRTGETPTKKRSMALEEEQQSPAKRLCQENQTALLRRLQDVANDRNSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHB4</accession>
    <name>A0A3B4DHB4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">P/Homo B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHB4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022309"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034468.2">
        <property type="protein sequence ID" value="ENSPNAP00000022309.1"/>
        <property type="gene ID" value="ENSPNAG00000029710.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157220"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035622">
        <property type="term" value="P:intrahepatic bile duct development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000001">
        <property type="entry name" value="Furin 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000034">
        <property type="entry name" value="furin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF11">
        <property type="entry name" value="FURIN (PAIRED BASIC AMINO ACID CLEAVING ENZYME) B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12 13" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017422609" description="P/Homo B domain-containing protein" evidence="16">
        <location>
            <begin position="27"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="724"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="17">
        <location>
            <begin position="444"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="686"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="686"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11 12">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="856" mass="93404" checksum="B91CE6AB7A73DD75" modified="2018-12-05" version="1">MEVRWVLLQLGTSLLLLSAVAQSAESQGFYTNSWAVHIHGGEEEAQCIARKHNFIYHGNVFGDYYHFQHRRVVKRSLSGHRGVHVRLHSEPQVVWAEQQVAKRRRKRDIYSEPTDPKFPQQWYLYNINRRDLHVKEAWAQGFTGRGVVITILDDGIEKDHPDLAQNYDPDASYDVNDRDPDPQPRYTQLNDNRHGTRCAGEVAAAANNGVCGVGVAYNARIGGVRMLDGEVTDVVEAQSLSLNSQHIHIYSASWGPEDDGKTVDGPAKLAKEAFLQGVTEGRGGLGSIFVWASGNGGREKDSCNCDGYTNSIYTLSISSTTQYGNVPWYSEACSSTLATTYSSGNLNEKQIVTTDLRKKCTDSHTGTSASAPLAAGIIALALEANMNLTWRDMQHLVVRTAHPAHLITSDWRTNGVGRLVSHSYGYGLLDASAMVALARNWSSVGPQHKCVIDMLTEPREVRNQLLFSKSVEACSGQLDFVSSLEHVQARLTLSYSHRGNLAIHLISPLGTRSTLLAPRPQDNSAEGFNDWAFMTTHAWDEDPRGEWTLEIENVAGLNDYGVLSQFTLILYGTGSSSADLSASDFSRPSNNSCKTFDAQQICIECSAGSSLYLQGCVKACPPGFTSGLQLLNLSLENWVEQSSVQACLPCHPACLTCSGPGEAECLSCPSHSHLVATSCLRQNQVQRKSPAGPTQNVQRGDGSDSDPDLDSDGPSPQKGLSSHLPVLVAVLSCAFILATFAAVFVLLQLHSGAANASFYRRTKLPVTDSSGRGMRVAFGFGLSRDWTSSRVAYRGIPTVWGDEDTAGGMNSESDSEELDCHRRASWCSHFLSAVLPCPGLLREELRSKNTQHCACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJX2</accession>
    <name>A0A3B4DJX2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CLN6 transmembrane ER protein a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017540090.1">
        <property type="nucleotide sequence ID" value="XM_017684601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJX2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023763"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011818.2">
        <property type="protein sequence ID" value="ENSPNAP00000023763.1"/>
        <property type="gene ID" value="ENSPNAG00000008272.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412537"/>
    <dbReference type="CTD" id="570764"/>
    <dbReference type="GeneTree" id="ENSGT00400000022240"/>
    <dbReference type="OMA" id="GESINHR"/>
    <dbReference type="OrthoDB" id="9970199at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029255">
        <property type="entry name" value="CLN6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16244">
        <property type="entry name" value="CEROID-LIPOFUSCINOSIS NEURONAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16244:SF2">
        <property type="entry name" value="CEROID-LIPOFUSCINOSIS NEURONAL PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15156">
        <property type="entry name" value="CLN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="36056" checksum="5E3EE31726B90F2B" modified="2018-12-05" version="1">MQPTVRKKPDAAQQLAFLPSHRQGSRDIDFSMKRPQFQFDLWCFFTIQNWVLDFGRPVVMVLVPLEWLPLNKPIVGDYFHMAYNIITPFLLLKLIDHSPKTLPLCAVYLCIITFVMGASINLVGDSMNHRLLLSGYQLHLSVRENPIMKNLNPPSLVDSFELLYYYDENFGRCMWYIPFFLILFLYFSGCFTTLKEEKRMPVSAWLLLAPSSLYYWYLVTEGQIFILFIFTFFAMTATVMHKKRKGLVPDSNGLFLLYSFSIALVLLTVWVACLWNDKILRRKYPGVIYIPEPWAFYTLHIRDKHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKW6</accession>
    <name>A0A3B4DKW6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4174 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKW6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025022"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008689.2">
        <property type="protein sequence ID" value="ENSPNAP00000025022.2"/>
        <property type="gene ID" value="ENSPNAG00000009880.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173711"/>
    <dbReference type="OMA" id="FTMLVMK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010811">
        <property type="term" value="P:positive regulation of cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025232">
        <property type="entry name" value="DUF4174"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46792:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 80-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46792">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13778">
        <property type="entry name" value="DUF4174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="DUF4174" evidence="3">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025022.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="137" mass="15352" checksum="0955817B9F30D367" modified="2024-11-27" version="2">MGIRHFALLKLVGKGPAASGTVELFPLNGRSQTEREQLSQDVVKNLRDQLKINRDYFSMLVVGKDGDVKAWFPSPVWSMDNIYDLVDSMELRQQEEKLQHTLGIHCPEDSGGGGGGGSFLGYPEETEDSYLYRRSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLJ8</accession>
    <name>A0A3B4DLJ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin 13a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017552215.1">
        <property type="nucleotide sequence ID" value="XM_017696726.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLJ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024335"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010378.2">
        <property type="protein sequence ID" value="ENSPNAP00000024335.1"/>
        <property type="gene ID" value="ENSPNAG00000008863.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426913"/>
    <dbReference type="CTD" id="798836"/>
    <dbReference type="GeneTree" id="ENSGT00940000157010"/>
    <dbReference type="OMA" id="NCCGFSQ"/>
    <dbReference type="OrthoDB" id="5845060at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF203">
        <property type="entry name" value="TETRASPANIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22686" checksum="E103E53C66457AED" modified="2018-12-05" version="1">MGCGGFVCSKNALCALNILYALVSLLMIAVAAWGKWFGLVSSFQVIAAVIGVGFFLFIVAIVGLCGALKHHQVLLFFYMVILFMVFIVQFSVSCACLAINKEQQNLFLEIGWNKSESMQKDLERTLNCCDFSSVNYSGTCEADCFREKSCEPCSVKIQAYADDALHFVGGMSLFFSFTEVLGVWLTYRYRNQKDPRSSAGQCRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLX3</accession>
    <name>A0A3B4DLX3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">All-trans-retinol 13,14-reductase</fullName>
            <ecNumber evidence="15">1.3.99.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RETSAT</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2</text>
            <dbReference type="Rhea" id="RHEA:19193"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:52075"/>
            <dbReference type="EC" id="1.3.99.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.23" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLX3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024111"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010954.2">
        <property type="protein sequence ID" value="ENSPNAP00000024111.2"/>
        <property type="gene ID" value="ENSPNAG00000008597.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017613"/>
    <dbReference type="OMA" id="THTSLVW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051786">
        <property type="term" value="F:all-trans-retinol 13,14-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052206">
        <property type="entry name" value="Retinol_saturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091:SF1">
        <property type="entry name" value="ALL-TRANS-RETINOL 13,14-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091">
        <property type="entry name" value="BLR7054 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024111.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="572" mass="63846" checksum="5D973B6B6535DBD6" modified="2024-11-27" version="2">NLLFWVLFCLAMLLHVPLLFNIPVIYEKTTSCAFLLSSTGFLASRVPRDLDAIVIGSGVGGLGIAVLLAKVGKKVLVLEQHDRAGGCCHTFSEKGFEFDVGIHYIGDLVENKPQRSNPFDHVVLGPPENRRVYPIYSCKNRYAQELKKCFPGEEKAIDDFIRLTKVRGVWLMPVLKIILSPIAKFLVYSGLISRLSYFFCYGSRSLAEVVNSLTQNEDLRAVFCYIFGTYGNIPKEASFSMHSLLLCHYMPGAWYPKGGASEIAFHMIPIIEKAGGAVLVRAPVNQSVLTLQWCVFAGVSVMKGHEEVHVHAPIVISDAGVFNTYQQLLPKEVQQLSFVKHGEGGLSVFLGLDGTKEELGLEAKNYWVFPENNLDAMEGYRKEEREEAVKSVPLIFIALPSAKDPTWEQRKPGRVWIPGHSFAPYSWFEEWKDDKVKNRGADYKELKETLLNSLLEMFSWLMLSRFLSLSLQIKYMDAGTPVTNQHYIAAPKGEIYGADQGTARFSAEFCVSIRPQTPIKNLYLTGQDLFSCGYAGALAGAMVCGSVILKRNLYVDVLALCMKLKYGSKKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DNV5</accession>
    <name>A0A3B4DNV5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RIB43A domain with coiled-coils 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RIBC2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIB43A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576530.1">
        <property type="nucleotide sequence ID" value="XM_017721041.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DNV5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024796"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009218.2">
        <property type="protein sequence ID" value="ENSPNAP00000024796.1"/>
        <property type="gene ID" value="ENSPNAG00000009547.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441489"/>
    <dbReference type="CTD" id="26150"/>
    <dbReference type="GeneTree" id="ENSGT00390000010825"/>
    <dbReference type="OMA" id="NLCRAIN"/>
    <dbReference type="OrthoDB" id="429119at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008805">
        <property type="entry name" value="RIB43A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517:SF10">
        <property type="entry name" value="RIB43A-LIKE WITH COILED-COILS PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14517">
        <property type="entry name" value="RIB43A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05914">
        <property type="entry name" value="RIB43A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="43064" checksum="09F0C81431FF24CC" modified="2018-12-05" version="1">MDRVQLLSDRVAAAHLDRSRKRELQRQERIFNARARTVGIDTDALDHQVQEKRSKEEAEAKALKEHTDGLIRSDRTACVLERRQKKDGRLLEEAIVRFRQQFQQPSLRREFDLNDPELLKKQDGVRILPGLTGEDLDSGERARRQREQLRDWSIQQQLELEQAKELQTLEDQQYDRSQLALDSRALELQKMEEEHKRAAAIAIKDFNQALAAGVMKRRESERHKEEENNQADVLNQLQGELLSESPQRVPGLTRVRRDCYKGLTPQQLMHYTNYQQQQAEEKRRARVQQREEELQQDRERLASARAALLQERRQERISKELRRALDNTNTQLAQMQKAQRNFLEKEVYTNSADESYFAQFNKTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DRI4</accession>
    <name>A0A3B4DRI4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SEC62</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DRI4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025574"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007300.2">
        <property type="protein sequence ID" value="ENSPNAP00000025574.2"/>
        <property type="gene ID" value="ENSPNAG00000010721.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002757"/>
    <dbReference type="OMA" id="TNDAYVW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="227"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="106"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="289"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="106"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="370"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="382"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025574.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="405" mass="46944" checksum="80AF2474C79A8FD3" modified="2024-11-27" version="2">ERESRKRKKRNNKKRLKEVSEPTKEEKAVAKYLRFNCPTKSTNMMGHRVDYFVASKAVDCLLDSKWAKAKKGEEALFTTRESVVDYCNRLLKKQFFHRALKVMKKKPEKDLKKEKEKEKARSDSGKEEEKKGKKEKEKKKDSEALDTKKEKEKNDDSPGSPKKKKDVKKKFKLEPHEDQLFLDGNEVYVWIYDPVHFKTFAMGLILVIAVIAATLFPLWPAEMRVGVYYLSVAAGCFVASILLLAVARCILFLIIWLVTGGRHHFWFLPNLTADVGFIDSFRPLYTHEYKGPRSSGKKSSGDKADGKSLESSKAQKSDSEDKSDSEKKEEEEEDDEEVVKETERQNSDTEDSERREDEGSQHSNGNDFEMITREELEQHTQDEEEEEEEEEEEEEEETKSLTVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTH7</accession>
    <name>A0A3B4DTH7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Lin-28 homolog B (C. elegans)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LIN28B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570205.1">
        <property type="nucleotide sequence ID" value="XM_017714716.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTH7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027677"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001942.2">
        <property type="protein sequence ID" value="ENSPNAP00000027677.1"/>
        <property type="gene ID" value="ENSPNAG00000002632.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437558"/>
    <dbReference type="CTD" id="389421"/>
    <dbReference type="GeneTree" id="ENSGT00940000153295"/>
    <dbReference type="OMA" id="EESRMIP"/>
    <dbReference type="OrthoDB" id="422005at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000007">
        <property type="entry name" value="Protein lin-28 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000087">
        <property type="entry name" value="Protein lin-28 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051373">
        <property type="entry name" value="Lin-28_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054081">
        <property type="entry name" value="Lin-28A-like_Znf-CCHC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109">
        <property type="entry name" value="PROTEIN LIN-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109:SF3">
        <property type="entry name" value="PROTEIN LIN-28 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21890">
        <property type="entry name" value="Lin-28A-like_zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CSD" evidence="13">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="12">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="94"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="24179" checksum="B415BAF875DD888C" modified="2018-12-05" version="1">MAEGGPGEGKGGGGDPAKTPGPEERTQSGSGYCKWFNVRMGFGFISMTHSEGSPVEPPLDVFVHQSKLVMEGFRSLREGEQVEFTFKRSSKGLESLRVTGPGGGPCSGSDRRPKGKAPPLKRKPKGDRCYNCGGLDHHAKECGLPPQPKKCHYCQSVMHMVAQCPHKGALSPSTSQDPHLSSAASTQSQEESSRSGSSPREGPSSSPEEAYQRAAHGQRWRKTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTR9</accession>
    <name>A0A3B4DTR9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibrosin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017580831.1">
        <property type="nucleotide sequence ID" value="XM_017725342.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017580832.1">
        <property type="nucleotide sequence ID" value="XM_017725343.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000027767"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001699.2">
        <property type="protein sequence ID" value="ENSPNAP00000027767.1"/>
        <property type="gene ID" value="ENSPNAG00000013324.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444267"/>
    <dbReference type="CTD" id="64319"/>
    <dbReference type="GeneTree" id="ENSGT00940000161032"/>
    <dbReference type="OMA" id="HSFFPQY"/>
    <dbReference type="OrthoDB" id="10060000at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023246">
        <property type="entry name" value="AUTS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429:SF24">
        <property type="entry name" value="FIBROSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429">
        <property type="entry name" value="FIBROSIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15336">
        <property type="entry name" value="Auts2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="392"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="704"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1033"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1254"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
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            <begin position="360"/>
            <end position="369"/>
        </location>
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        <location>
            <begin position="401"/>
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        <location>
            <begin position="832"/>
            <end position="844"/>
        </location>
    </feature>
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        <location>
            <begin position="858"/>
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        </location>
    </feature>
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            <begin position="923"/>
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    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="995"/>
            <end position="1004"/>
        </location>
    </feature>
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        <location>
            <begin position="1259"/>
            <end position="1272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1291" mass="137836" checksum="EF61BBC90F6FA594" modified="2018-12-05" version="1">MDGPSRSGGLRQSRRSRSQRDRERRRRRANLGPSSPSSASDQELCRGDSLLGASGGECRPVFPGARHRPPRRRKRESVSCEEDIIDGFAIASFISLEALEMDCSLKPPERAGMLMGRGNKRKRGVDENGGPLSEREEGAPATYTTSSWEKRKNEKRKREAKVSGNLMETGYICDTESESGDKASDNDMEPTFIVSTREVVSNPVSMASSVSNGCTLLPSNSGPPRLSVTPRVSGLERSQERSLELPYPEATSSGLPGLLPRSPASVATPPSEQHNGNAGPHQRSPPRQKPFLPFPSRTQTVFSNGNRSGTSVKTQSSSASLSIRPPTPSTSVPAGRGPSGTGVLRPSSRPSPGALFTPSPGLPPPPPLLQSPRSATDQDLYRQDLNTHFLASQGAERENRASGSSAGVNSSSGSGRPSASASSSSTSSRTSQAQASIPAMYQFHHHNHQHQHTHTHQHFLHPPAAAPPMFEKYPGKMDGLFRHPFFSQYPPPVPGIQPVLPPTGPLSSLQGAFQPKLLALQGPAPEMTARLGVVPPHLQPKDPRISDPFAPSLKVSNKPGKWCAMHVRVAWMILRHQEKVKLMQADPQKLDFRNDLLPRLPGPGGIGGIGGLGALGGPLPPTHELARPGSLFAAAGGVNPSSTPFISPSTPHSSFLTPATHLDPYGRSPPFPPLGALGSGAFGGLGSPSLTASSVFGHKAESSASAVGGLGNPHEQWNRLHVAPPSFPSGPSWAKGLEKRDERERGKDMERRELTHIKDEKDRDSLLYGRPPVRMSPSAPSLKHRSSTPSSHMNGLGPLSGGAGQSDGQSREREREREREREREPDKRHHSVSASRAQASTSSAPDRPRSSTSSVLATPPPNAPSAPSPLDLFPRQAHALNSEPSHPSQRESSGPASSSSSVSSLPVKKPDRTTTPVSKQPAGLPPGLPHPPVKVKEERKEEPEQVPISLPHPGIPPHSFERPNSRHTHHPSTPSSTLSLTPTPGVPLPPPTQHPTHHPTHHHLSLLDRTRAIEAYLGGAGGSAGLVVGAPGDRFSVHPHTPPQGHSQPPHSFPWDTWRDLAAQQQQHRRESVLALQPDPHLALRSDPHFSRLFQHPHARYLEAERAAAVAAAVAAGQHHPQAPTSTASSSVARPEFGLMSHPFDRPPQVGGPGGGLLDEDQRTQILREDFERVRYFGMHPHLAAPHLPSPSAAHLEQLHAGLLSHSQLHPAGASAPSPHHSLYSRLGPLHPHVPNGILAKAPSLVGPLSVGAPPPLIPSATRPSTPPRSSRLGGAGDLALFSTHKDGESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZ87</accession>
    <name>A0A3B4DZ87_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RRAS2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556138.1">
        <property type="nucleotide sequence ID" value="XM_017700649.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZ87"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029727"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017119.2">
        <property type="protein sequence ID" value="ENSPNAP00000029727.1"/>
        <property type="gene ID" value="ENSPNAG00000016021.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429124"/>
    <dbReference type="CTD" id="22800"/>
    <dbReference type="GeneTree" id="ENSGT00940000155328"/>
    <dbReference type="OMA" id="NKRGCHC"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04145">
        <property type="entry name" value="M_R_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="202" mass="23198" checksum="1A1617997232EE69" modified="2018-12-05" version="1">MAGWKDGSVQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDERPARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILVGNKADLELQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKSGCHCVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E000</accession>
    <name>A0A3B4E000_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E000"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028641"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014998.2">
        <property type="protein sequence ID" value="ENSPNAP00000028641.2"/>
        <property type="gene ID" value="ENSPNAG00000035181.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF538">
        <property type="entry name" value="OLFACTORY RECEPTOR C FAMILY, H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043837102" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="552"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="622"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="667"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="706"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="745"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="777"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="552"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028641.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="820" mass="90884" checksum="0BF34894905985D4" modified="2024-11-27" version="2">MFLYAFLLLYVFKTKGENAKCQMIGSPEYPVLSKDGDVIIGGAFSVHSNITLETMLFTEKPQGLTCTSLDFREFQFVQTMIFGIEEINNSSLLPNISIGYKIFDSCSSTLVSMALINGQELTAQETCSGHSAVQAIIGESESSSTIVMLRATGPFRIPVISYFATCACLSNRKEYPSFFRTIPSDYFQSRALAQLVKYFGWTWVGAVRSDNDYGNNGMATFMEEARKEGVCVEYSEAILRTNSKQSIAKVVEVIRQGTAKVLVAFLAQSEMDVLLEEALAQNMTGLQWIGSESWITARYLATEKTSKVISGAIGVTINNYKIPGLKDFLFTPKDENEKLCTGFENLKEINNTFTDVSELRISNNVYKAVYAIAHALQNTLACTADENITCTKKDALLSSQVLYSLQSVNFTMPTGESVYFDSNGDPTARYELVNWQKNPAGDIVFITVGHYDASLPSDEQFVMKPVSIVWAGNSDMRPRSVCSESCQPGFRQAQIKGRPPCCFACVQCPVGEITNETDSVECIRCPLEYWSNENHSLCVPKKVEFLSFDENMGILLTVFALTGVSFTVAVGLVFFYFIDTPLVKASNTELSFFLLFSLTLCFLCSLTFIGQPSKWSCMLRHTAFGITFALCMSCVLARTIAVVMAFRATVPGNSLPQCSMPVQRMSVFCCTLFQVIICTLWLALAPPVPQKNMAHYTDKVILECDLGSVVGFWAVLGYVGLLAALCFVLAFLARKLPDNFNEAKFITFSMLIFCAVWITFIPAYVSSPGKFTVAVEIFAILASSFSLLLCIFIPKCYIIILKPEQNTRKKLMGKTHVKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0L9</accession>
    <name>A0A3B4E0L9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">VLIG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541756.1">
        <property type="nucleotide sequence ID" value="XM_017686267.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000028861"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015421.2">
        <property type="protein sequence ID" value="ENSPNAP00000028861.1"/>
        <property type="gene ID" value="ENSPNAG00000003216.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413650"/>
    <dbReference type="GeneTree" id="ENSGT00940000163472"/>
    <dbReference type="OMA" id="KYACWSI"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058641">
        <property type="entry name" value="GVIN1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796:SF6">
        <property type="entry name" value="INTERFERON-INDUCED VERY LARGE GTPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796">
        <property type="entry name" value="URG4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25974">
        <property type="entry name" value="URGCP_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="10">
        <location>
            <begin position="691"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1046"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1636" mass="187631" checksum="D29E70869A2839E6" modified="2018-12-05" version="1">MSGRKMDAQSPGRNTAERTSVTAQERGTAVVPVIDRNNIQGPLTVNINHIYRDDRSTGAAGQDGVGMKSADGTQKKDSSIKIPDFIYRLHLSNFYPSKLTTANAFNISRTSFLKEEPQSEKKPLKVEDLAFVYLHKLLTLDYRARSLTIHNECVTTKTGKRSAENKDFYSFLNDECEYTDNEDGNTQIHPMDVQMAVFYCADSFLRQNMVTKLSSCQYALPLLVPSPTSTDIELPLWTFRQIKKIWKSVGGLSTSLPVCQIKAPMVFFCRLGSVSTSKSELLNSVINPKHDTFFHRDSPGSSRSRLFMEGLVEIAWYCPAGNTDDLFSECIAFCNLHGDAVKNRKQTNFLSEQATVNVVLMENTKKHNDKDREMVQKLHNLSKPLICLMSEQKGTNTMGNGYNIKYKIGLRDRNQAELHEELISVISTCLQKCNSAPTFSLEASTELAKSYGFRVDEDSQECQAGKTGALEVIKLLKGGDISRIKAKFLPCQGKLWQDWSRKNKEQYRLYGDVEQTKSKIQDHMKSIRNQQRRTSLDLMKAVCKNLTFKQISEKMYFVRWLGVYLDVSFLEKLSEVTQQYYKQLSDLQILKQKREITDEFRKKQEELIQLSEKVDAVTFGLEHIVREMGQIYEAWAETPDRMTAEVSILPVLAADLLISGHPLELMDGDAAHVPLTWIQSVLDKVTERLGDQRVFVLSVLGLQSSGKSTMLNAMFGLQFAVSAGRCTRGAFMQLIRVKEEVKDQLKFDYLLVVDTEGLQSLQLVSKQSGLTHDNELATFVTGLADLTLINIFGENPAEMKDIIQIVVQAFLRMKKVKLAPSCMFVHQNVADISAGEKNMEGRSQLQKQLDEMTHLAATQEVCSAEFFTDVIKFNIQTDVHYFAQLWEGNPPMAPLNPRYSENIENLKQAILKTAAGKKCLKLSELKVRIKDLWSAVLNEDFVFSFKNTLEISVYRRLEVMYGNWTWELRESMLFIEKKLHNWVENGKVVAINQRRIHEEISGTFCAVKNKTEKFFAEDKDAEILSQWKARTEQRIKAVHDSLVEAAARKMDELIKLKKACKKLDEKKTQYENDLFNRSKDLASTLEDMDMNERSLCREFDSMWGKWLSDLRAETPPIKDVKVEQEVMNILSSYFERRTIYSNRDSGHYKWIYNRRDYSDYVTVKKGTFGISYYSLDTKDHDSVRKLVQQTVREAKEIIRTKPVHKTGYSDAYTQEIVNCVKKRVAEFKAEKFTLKNEFTVDLSLYVCDIAVRRFTDLHKVFRDANDPVIYLGSKKGDFFSIFKKQCEGVSATVVFADFVCNKLKPSILQSVYDQTAIDAAGEMRANFPAFNGNRSNLEKHILLDLAEAESFERFMTYIEDPKKHFSDFIETEFKEQMLEGRNPRILKILRDAANMKHSSVSTAIQKATEETKRKKDVHEWLKRFSQELEGEVIFTVNDVEGAHYQENTETDFFGEVMNETLHSVFNEMTSSFDHISSIRMDVFKPEIRPEDILKNQLCGCWVQCPFCAAICTNTIEGHDGKHSVPFHRPEGVTGWKWYKTDNLSVDICTSSVVSDAQIVLPDEKCVPYRKYWEAGPKYACWSITPDTSEQAYWKWFVCRFQRDLEKHYGKTFKGKGEIPTAWRNITKQNAIESLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1B0</accession>
    <name>A0A3B4E1B0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ANXA2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1B0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029024"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015759.2">
        <property type="protein sequence ID" value="ENSPNAP00000029024.2"/>
        <property type="gene ID" value="ENSPNAG00000015429.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154257"/>
    <dbReference type="OMA" id="QQDTKND"/>
    <dbReference type="OrthoDB" id="37886at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004859">
        <property type="term" value="F:phospholipase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000007">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002389">
        <property type="entry name" value="ANX2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF18">
        <property type="entry name" value="ANNEXIN A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00198">
        <property type="entry name" value="ANNEXINII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0041">Annexin</keyword>
    <keyword evidence="8" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 11" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029024.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="358" mass="40916" checksum="00CECD06502763D0" modified="2024-11-27" version="2">MFILEHTWIHCKIISSREVRMALVSEFLGQLSLGHGGLREPRCPTVFPAPNFDPEKDASWVDSAIKTKGVDEHVIIDILTKRTYMQRTEIAFEYERKEKKDLVTALKGALSGSLENVILGLMKSTTQFDAFELRASMKGLGTDEESLIEMVCSRSNEELVEIKRVYKDLFKKDLEKDVAGDTSGDFAKLLLALVQARREEPSNVVDYEKIDSDARALYEAGVKRKGTDVGTWITIFSERSVPHLRKVFERYKSYSPYDMKESIRKEVKGDLEKSFLTLVECFENKQLYFANKLNEAMKGKSAKEKILTRIIVSRCEVDLMKIRSEFKRQFGKSLFQTIAEQTKGDYQRALLNLCGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4W2</accession>
    <name>A0A3B4E4W2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">FAT atypical cadherin 3b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000030738"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019051.2">
        <property type="protein sequence ID" value="ENSPNAP00000030738.2"/>
        <property type="gene ID" value="ENSPNAG00000003491.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154981"/>
    <dbReference type="OMA" id="NDSPRNG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048667">
        <property type="term" value="P:cell morphogenesis involved in neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5Q0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018680.2">
        <property type="protein sequence ID" value="ENSPNAP00000030564.2"/>
        <property type="gene ID" value="ENSPNAG00000017348.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182860"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13233">
        <property type="entry name" value="PH_ARHGAP9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04403">
        <property type="entry name" value="RhoGAP_ARHGAP27_15_12_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000003">
        <property type="entry name" value="Putative rho GTPase-activating protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000182">
        <property type="entry name" value="Rho GTPase activating protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000101">
        <property type="entry name" value="Rho GTPase activating protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF103">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="261"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="329"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="8">
        <location>
            <begin position="507"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="147"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="445"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030564.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="80526" checksum="35E9177B6B6E2B55" modified="2024-11-27" version="2">MLSGSWRRNTAGPTCSLPIGRGSVSGGTVVLEAQYDYSYRGVDGRVVSIQEGERFLLLRKTNADWWQVRRLGVSMKSKPLYVPATYVIELPISTLHTPQPSSQSAASPRRLVARGSYTPCSQTQCSGPSKAFCHSMEDLNSNSAFTNTRVGRSSAGGHFPTLPLSSSSTSPSGHLTVPGGPLGSGGLIPRSQSSNNLPQNPYEEAYRSRPGPTSPTKSYSQWDVAASARRHTDGHIQGRRQWAEPPCPLPGQKPLQILQLWEQYQDPATGRYFYVNTVTRERSWKPPRRARQSDHGQSMITCQVGTFPRDTSHLSLPLHNSGSKSLHRELEKAGLLNKTKIAEGGRKLRKNWNPSWVVLVGNSLVFFKDPKTQSPASWKPGNSCPESSVDLRGAQLQWANDLSSRKNVFKLRTVTGNEFLLQIKENPLDNVLLYSLRRAGSMEMLDQSGDEDENRVGSKASLPRSSSNLENSERKRVKSRLKKLILRRPPLQALQEKGLIKDQVFGCSLEVLCDREKNTVPRFVRNCTETVEKKGLDTDGIYRVSGNLAVIQKLRFAVNHERAVSTDGRYLFPEDLVQGNLEDPQWEDIHVITGALKLFFRELPEPLVPYGFFHDIVETVKMSDYLDKVDRMKLLVISMPPPNHDTMRHMFRHLKRVMEHSDSNRMTTQNIGIVFGPTLMRPQSDCGNMAVNMVYQNQAVELILSEYDQIFGPDLSDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8L7</accession>
    <name>A0A3B4E8L7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May play a role in the development of the central system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHYHIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570017.1">
        <property type="nucleotide sequence ID" value="XM_017714528.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8L7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036825.2">
        <property type="protein sequence ID" value="ENSPNAP00000031604.1"/>
        <property type="gene ID" value="ENSPNAG00000019062.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437438"/>
    <dbReference type="CTD" id="100004913"/>
    <dbReference type="GeneTree" id="ENSGT00390000014563"/>
    <dbReference type="OrthoDB" id="6101761at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000277">
        <property type="entry name" value="Phytanoyl-CoA hydroxylase-interacting protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042868">
        <property type="entry name" value="PHYHIP/PHYHIPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045545">
        <property type="entry name" value="PHYIP/PHIPL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15698:SF8">
        <property type="entry name" value="PHYTANOYL-COA HYDROXYLASE-INTERACTING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15698">
        <property type="entry name" value="PROTEIN CBG15099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19281">
        <property type="entry name" value="PHYHIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="52"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="377" mass="42451" checksum="4E759668E4EB7500" modified="2018-12-05" version="1">MEVPRVGHSVSSPTSPCEDMMKNLSLDAIQLCDREGNKSQDSGIAEMEELPVPQHIKISNITCDSFKICWDMDSRAKERITHYFIDLNKKENKNANKFKHKDVPTKLVAKAVPLPMTVRGHWFLSPRTEYTVAVQTASKQGDGDYAVSDWSEIVEFCTADYSTVHLTQLLEKAEAIAGRMLCFSVFYRNQHKEYFDHAREAQNNKMLPSVKDNSGSHGSPISGKLEGIFFSCNTEFNTGKPPQDSPYGRYRFQVPAEVLFNPGTNLYFADFYCMYTAYHYVILVLAPRGSPGDDFCKQRLPLLDIADNRFLTCVQDEDGRLTFRHAQDLILEVIYTDPVDLSLGTVAEISGHQLMSLSTVNAKKDPSCKTCNISVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8U2</accession>
    <name>A0A3B4E8U2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574321.1">
        <property type="nucleotide sequence ID" value="XM_017718832.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8U2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031691"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020884.2">
        <property type="protein sequence ID" value="ENSPNAP00000031691.2"/>
        <property type="gene ID" value="ENSPNAG00000019172.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440108"/>
    <dbReference type="GeneTree" id="ENSGT00390000005094"/>
    <dbReference type="OMA" id="SFCYERR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040893">
        <property type="entry name" value="RADX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14944">
        <property type="entry name" value="RPA-RELATED PROTEIN RADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14944:SF3">
        <property type="entry name" value="SI:CH73-71D17.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17659">
        <property type="entry name" value="RADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031691.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="812" mass="90470" checksum="534C49DE83795D28" modified="2024-11-27" version="2">MAAPSSLSDGCALERCLNRLTAASSPSLKAKSQAEPLYMISLERYGRDPLFGVHFPDSISESESLFDAVLTDGHCRVKVALDPSLNKLIHRNQLRCGSALRNVVFSAGGDDSQAQGRTFTVCSLEVDSLCGVDAALRALSSVTVSSLPWLGSEPSSLPLRARRSTYLPLWNNHDFTGDVWRDTPPSEHGEGESEDEAAEAAGRPVLSLAELRRRFLKGSTRPRGSLCVRILHKSRLMYYGKAEQSCECPYKAELQVADGSLSVCVVLWNTVCLDWYRCLQPGQVLRLSRYRVKESYSSRSAHNAEPNIEISLNSRNPSAEVSIIPKQQVSPQWSLPSLPHNFLCGKELLSCPPSNICDVIGVVTFVGRQERVRKKDGQRTEFEEYRWLRLEDGTSDQPITIKLFSTSQPEIQSRIQPMALLVSTRLKLIRNTVGRATAFEYLTNTPITQVYCTGTGSHPVMPYRRLRPIRQFLQWLKEVDEASMLDRAVIGGCFSYPPLPISLQSFMDNRGGEAGFISGGEMKLQCDRLHYREKRRFAVLCTITAACYHSREDSGRMRYSDPPSAQLSPRVPRQKESSDLFRTPTKRKLIFTAGTASSARKRLAPPLQQASTEEDLENEFSLFDGALEFLVGEEEDDDEDDDEDNESFSTAPTSPMSYHLGLTRVAMETLPRSFCYERRHVQAAAVGMQTSSFHKLLPHRELETFSPAHCYTGYFTLTMKALSDGVMLDVLFLPATPGNLHWRPLPITHDNTWDSVLSHGGFSPHAPPPTPADLIATATQLTNRRLVCVLEACALGGDKVELILNRAFPLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ED58</accession>
    <name>A0A3B4ED58_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HEAT repeat-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017568932.1">
        <property type="nucleotide sequence ID" value="XM_017713443.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ED58"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033229"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023917.2">
        <property type="protein sequence ID" value="ENSPNAP00000033229.1"/>
        <property type="gene ID" value="ENSPNAG00000021718.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436760"/>
    <dbReference type="CTD" id="399671"/>
    <dbReference type="GeneTree" id="ENSGT00390000013207"/>
    <dbReference type="OrthoDB" id="5980716at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF20">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="931" mass="104525" checksum="096730C9CD40BFE5" modified="2018-12-05" version="1">MDLQQPSKGSMEYHHSRRGKQLYRQLLRNASEGLSFSQDVMWEMGADGISYSKADFSWLFRATGRLAPDTKPKSRKKAGTKVSPCHIGPKAQEKENELVGVPLPVLPPLQTTHYSHNSLTTKEPFTIPNQQGGMLVCFQRTELRGASNVYSCLKKVPPDVREEYSRNSKWNEFVLKKLTKTTAQWIVSQKIPKQSQHKEGLQSLLKGQYGSASATDLVTDEPMREADFCGFYDAPRSSSEPKCLQSSKAETPLPVYYRIQGYVFSPVCTDEQGGSNQTANTVTVKHTETPKPPRLQNSLNPHAGKYVYYTKNDFEQELYSGIAKQVHQRDRRNEDRIILDNNSEYQKNLQELFPCGPEKWTHVTEAKMGRVEKGLRRWMDLPTKADYGTELGLRPPVYTGQTTEVAKQQVHYKLIPELSSLRYAVEEWRNAWKIKITWQSVTVEGLKKSLTDLHCHVRLAAIATCALGAVHRPQEELAASETALYGRGWEVEPVPPELQPLLLSALSDPVKRVQIAAAVCQYAMGASNPHAQDILRNVLHQDTSGMGADSWVAAQCLALEGEATQAVVERLLSQHFLSEAPADQDQAATLLSNISSKTTLVRSRLSEELNCGNWRTRVLACNTLSQLKCQINKDLSNKLSHLMWNDWSSVVRQAAAQALGRLNLGSDLHDELIVKLDEGPASRRVEALVLIGQLKIMTIKLLPAFLRCLVDDFVVVRKQACFTAASLMLKDEIIMEQLIDLMHNDTSCDVKVVAINALGKIGCLTPALEELLLWALHHEEEPRVRIAACEALKTLGVKGPELQLILQERFVLEPNPQVHRHIEAMLKSYGYSLEGDKGMVYRITDQVQQLCSKNIITEKVLLLEELTQLQQHQRRLLGQEGQPEASVMSALLQERFTALKHLSTRSLSASKDGQDKQYTTAHMSGWTLKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EDZ1</accession>
    <name>A0A3B4EDZ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain containing 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCDC14</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017557654.1">
        <property type="nucleotide sequence ID" value="XM_017702165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EDZ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033938"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038248.2">
        <property type="protein sequence ID" value="ENSPNAP00000033938.1"/>
        <property type="gene ID" value="ENSPNAG00000022905.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430024"/>
    <dbReference type="CTD" id="64770"/>
    <dbReference type="GeneTree" id="ENSGT00390000017916"/>
    <dbReference type="OMA" id="QACHRET"/>
    <dbReference type="OrthoDB" id="10014807at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071539">
        <property type="term" value="P:protein localization to centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029343">
        <property type="entry name" value="CCDC14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22367">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22367:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15254">
        <property type="entry name" value="CCDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="666"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="464"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="670"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="688"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="909" mass="99779" checksum="8116F4107B9C50CF" modified="2018-12-05" version="1">MARQVKSRHKVVSSGRLTGCGQGQVNKKTVVGRHAAPLEPAYSLYSTDSEDQVTTLHKGLDRCATLLSGILQAEKTESKSKPQVPKTVSSKLKSKGVHGKLDTERKRLGKKSSSVSHITKRPAVVQKTILSSQTYPGLRQRPLAVDVVHGLDVKPRALTPAAQSEHIQPHSLPSLCPVSGGSNSLPSTGLQPSTVFNCRLTTSTPVLSPQRLTSAQSQACHRETDGEMHYFPQQWDIATKGQSQQHGPLNTAGPPTQYTALLSVSPAVQQHPPASTVSTVHQASIRPMAKGELRVTSPLLSAPHVLSVGQSSPLQSQNGSQYCPSPTVAAHTPTQVPITSHPQTYPQPVLVHSSHQEQSSGAEESSEESGGCTSEEDELAGVDTTPVRDTSCQTSMNKHAVQSSPEKTARKVMTVKYLLGELKTLVANQDSEAVRLISEVEQSISLLPAMVGSTNVQAELALALQPLRSENVQLRRRLRILNQQLMERERAERQARSVDCNLELATLQSLNITLQTQLNDNHRELGNLQQENQRLQQALEDKERDLQQTKEQCEIETSRIRLDVSEALAEMRNCKAELEDSERENVALTQNLQQKEAEIIRLQEIIRTLQKRPTREIACQLTQTEVPNPPSQLTKSVLDLYENQQKETALTDAVTDSIKTYLQTLEDTGHGSSPQKSHPQPSSHCQWRGREERYRARDTKAISPKRLAVPRPAQDTSAGGGTQEAVRSESGIAFVPLKETLKAHPAAKLTQTCSRIQVNERNQLHEVNEHPGLKYLDLAFEKLGIVNGPLAQDLETLDDNRGKDFLSHADGVGSQLKRSSEGTVNFAEQSQNARRCLKMGEASTCTGRPSVFESTVSSCDIKSLASDWSLNSWSTFNTRDEQDFRNGLAALDASIESLQRTLKADLKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EF03</accession>
    <name>A0A3B4EF03_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017561415.1">
        <property type="nucleotide sequence ID" value="XM_017705926.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EF03"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034313"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026001.2">
        <property type="protein sequence ID" value="ENSPNAP00000034313.1"/>
        <property type="gene ID" value="ENSPNAG00000023548.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432223"/>
    <dbReference type="CTD" id="102216267"/>
    <dbReference type="GeneTree" id="ENSGT00940000164726"/>
    <dbReference type="OrthoDB" id="3908708at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15472">
        <property type="entry name" value="Myo5p-like_CBD_Rasip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06690">
        <property type="entry name" value="PDZ_Radil-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17116">
        <property type="entry name" value="RA_Radil_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037983">
        <property type="entry name" value="CBD_Rasip1/Radil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF4">
        <property type="entry name" value="RAS-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="3">
        <location>
            <begin position="115"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="4">
        <location>
            <begin position="597"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="1078"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1035"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1063"/>
            <end position="1073"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1175" mass="130480" checksum="A750863D5AF5D0CB" modified="2018-12-05" version="1">MISEERSNQLSKQTLGFPVGLLIRSPKKSLAKLGRKPSNGSLQSSASDITTRSMESTGVRQPAKSKIRRHNNRLATVFNRSPLLRDSRPGQGGRHLSGDLQVADDPSELSSQLSAPGILKIFGSDICQGANYKSVLATTRSSAKELVKEALERYCLEKEDPEAYVLCDVIGQTGVDHQWKTECLRFVGDNEKPLMLQSLWKPKEGFSRRFEIQKRTSVEEHSGKDRDTITAGLNAQVRKLQKVRSRVTSLFVDGSDGEIDSLGMWRSLSDMDVSTMGKEACKVHKAGALQEDPEAEADAEGRCLSTEKEETESSDDNTTQYSIHPPFDFPYFLLLQGYSHRQDFVIYLMSGNSCVFGCYGEHSEDMERLKVDVLLYAPDVLPQHCCVRRLDAPNPPSEGNRRTLTLLQPLHGAPITRNGVPLKSEAELFSGDLVGLGQHYLFMFKDPTAPDAQHMPSWMTTLCAPAEASPCKSCGSSLRKPQTHRNVAARWRDMEGRVLSLSYQLQQEDQVLEKILTMVDPRGEEPKLIPAFLLCLCIQHSAASFEMVHFRKLLLRIANQIQLAMWESTKELASLQPDSGSGDRQPEPEELHLLSMAELIPGLQPLVLWMANSIEMLHFIQHEVPLLLPWTQQDEEDQCKELSSQILSTRTASEEAMTVLEEVIMFTFQQTVYYLTKSLYAVLPGLLDSNPFSESGQLHMPMGVSDILDVLKEALQLLNTFQVHTEISSQLLAYLFFFTNASLFNILMERGSGGGFYQWSRGVQIRANLDLLMDWTQSIGLGDLAAEFFQKLSSAVNLLATPKETLLQASWPSLRTEFTHLNPAQLHHMLREYNAGRACPSCWTPSPEEAESALLTTEILESFENHPPLILPNSTFHLELGKPITEPGFFSQLQYLQEFIHTISISQMPNILQPLEDTSNHVMEDTQSISSPSGTEPECHIASVSPDPTINFAYKAEEQQCLSASPSHDRGSHGDLSSCEAVLAQKLKNLELQSSLPGETDIGYHKSLALDPSCLLTPPNTPQGLELAELEASLQEEATQQSARHQKKVGNGLVAAADQSDERNEEEDDEDERDEVFTVELSKGPHGLGLALVDGLKTPLRMSGIYVKSVVPDSPAAQCQRLRLGDRILAVNGVSLVGMDYHNGREIIRTSGDSLRLLVAKTEARSSGRTSITRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGU9</accession>
    <name>A0A3B4EGU9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Bone morphogenetic protein receptor type-2</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Bone morphogenetic protein receptor type II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Can also mediate signaling through the activation of the p38MAPK cascade. Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6. Promotes signaling also by binding to activin A/INHBA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="26">Interacts with GDF5. Interacts with BMP4. Interacts with SCUBE3. Interacts with TSC22D1/TSC-22. Interacts with activin A/INHBA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017547707.1">
        <property type="nucleotide sequence ID" value="XM_017692218.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGU9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034958"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027318.2">
        <property type="protein sequence ID" value="ENSPNAP00000034958.1"/>
        <property type="gene ID" value="ENSPNAG00000024607.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424301"/>
    <dbReference type="CTD" id="555655"/>
    <dbReference type="GeneTree" id="ENSGT00940000156449"/>
    <dbReference type="OMA" id="NTVAHRG"/>
    <dbReference type="OrthoDB" id="669224at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005024">
        <property type="term" value="F:transforming growth factor beta receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030324">
        <property type="term" value="P:lung development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040017">
        <property type="term" value="P:positive regulation of locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045778">
        <property type="term" value="P:positive regulation of ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014916">
        <property type="term" value="P:regulation of lung blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000026">
        <property type="term" value="P:regulation of multicellular organismal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001944">
        <property type="term" value="P:vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14054">
        <property type="entry name" value="STKc_BMPR2_AMHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23614">
        <property type="entry name" value="TFP_LU_ECD_BMPR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000180">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000006">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000174">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF63">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN RECEPTOR TYPE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="22" id="KW-0464">Manganese</keyword>
    <keyword evidence="18 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12 32" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 31" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="32">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017439091" description="Bone morphogenetic protein receptor type-2" evidence="32">
        <location>
            <begin position="29"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="33">
        <location>
            <begin position="210"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="573"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="674"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="746"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="890"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="573"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="767"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="900"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="956"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00057734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00063997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00073640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00077874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1059" mass="114802" checksum="D0EF3699B2A0E9F2" modified="2018-12-05" version="1">MDGMAVLGRVSVLALGLCAVLLLSPVAAAQSEERECAFNDQHQQPDEQSTAGDWRVMRENNTILCSKSSRCYGLWEKTHDGDIRLVKQGCWTYIGDQQDCHDDRCVVTTTPSQIQNGTYRFCCCSTNMCNVNFTENWVPSPTSATILDPRPLYREEAIFIALASVSIVAVLIVALFFGYRMLTGDRKQGLHNMDMMEAAASEPSLDLDSLKLLELIGRGRYGSVYKGSLDERPVAVKVFSYANRQNFINERAIYRIPLLEHDNIAHFIVGDERLTTDGRMEYLLVMEYYPHGSLCRYLSLHALDWVSCCRLAHSVTRGLAYLHTELLRGDSYKPAVSHRDLNSRNVLVKNDGTCVISDFGLSMKLTGNRLVRPGEEENAAISEVGTIRYMAPEVLEGAVNLRDCESALKQVDMYAIGLIYWETFMRCTDLFPGETAPEYQMAFQAEAGNHPSFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETMEDCWDQDAEARLTAQCAEERMAELLLIWDRNKSVSPTVNPMSTALQNERNLTHSRRVPKMGPYPDYSSSSYIEDHEGMVKNLPGDVSTSGTSSVSGMGVSGISTGTGGAGEKNRNSINYERQQAQAQARLPSPETSGASLSTTTTTAGLTPSTVMTTISELGHSEETGAGGSVPVCLQLTEEDLETTKLDPKEVDKNLKESSDENLMEHSQKQFSAPDPLSSGSSSLLYPFIKLAAEVTGGAGGGTQAEFGAGSSVMGDAPPAMFPLPKQQNLPKRPTSLPLNTKNPSKDSSSSRLKFGKHKSNLKQVETGVAKMNTVAAVEPHLVTLTNNGSAVRSGVGVVVVNGYVNSSSGAFGTTNPAGPQGEGGAPLLQSQLSGEDSRLNININSSPDEHEPLLRREQPAARDHTGRTNSNNNNSNIRPSAPVAPTRPAAAVPPTEPPAQPSVPQIKEEVPLRQPKPRRPERPNSLDLSSTSQDSSLSGEGSSQEGKAGSGEKIKRRVKTPYSLKRWRPSTWVISTDTLDGEVNNNGAGGSQGASGATRSKSSTAVFLVGGGTATATLSSDPPGMTCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHQ7</accession>
    <name>A0A3B4EHQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Syntaxin-binding protein 5-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">STXBP5L</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558534.1">
        <property type="nucleotide sequence ID" value="XM_017703045.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHQ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035283"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028042.2">
        <property type="protein sequence ID" value="ENSPNAP00000035283.1"/>
        <property type="gene ID" value="ENSPNAG00000025420.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430513"/>
    <dbReference type="CTD" id="9515"/>
    <dbReference type="GeneTree" id="ENSGT00950000182906"/>
    <dbReference type="OrthoDB" id="19944at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15893">
        <property type="entry name" value="R-SNARE_STXBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000521">
        <property type="entry name" value="syntaxin-binding protein 5-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000664">
        <property type="entry name" value="Lethal2_giant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013577">
        <property type="entry name" value="LLGL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF19">
        <property type="entry name" value="SYNTAXIN-BINDING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08366">
        <property type="entry name" value="LLGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00962">
        <property type="entry name" value="LETHAL2GIANT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="239"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="17">
        <location>
            <begin position="1201"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="564"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="726"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="796"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="879"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="735"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="805"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="879"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="892"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1266" mass="140429" checksum="D50D0F6325EC41BE" modified="2018-12-05" version="1">MKKFRKVLDGLTTSSPASAGGSPSCGSAAGTPTAAPTPRELDVHETLLSEHFHICKTVRHGFPYQPTALAFDPVQKILAIGSRSGGVRILGRPGVDCYCQHESGAAVLQLQFLINEGALVSACADDTLHLWNLRQRRPAILHSLKFNRERITFCHLPFQSKWLYVGTERGNTHIVNVESFVLSGYVIMWNKAIELSTKTHPGPVVHLSDSPKDEGKLLIGFESGTIVMWDLRSKRADFRIYYDEAIHSVSWHHEGRQFMCSHSDGSLSMWNLRNTAKPFQVTFPHGKSQRDGRKESCKPILKVEYKTCRNSEPFVIFSGGLSYDKAGRRPTLTIMHGKAITVLEMDHPIVEFTVLCETPYLNEVQEPYAVVVLLEKDFIVVDLTQSNFPIFENPYPMDVHESPVTCTAYFADCPPDIIPVLYSIGAKHKKTGYSHKEWPVSGGTWTIGSQTYPEIIITGHADGSIKFWDATAITLQMLYKLKTSKVFEKPKGGEVGRSAELVEEDPYAVQMVSWCPQSRVFCVVGISAHVILYRFSKHDANTSITSLEVRLQCEMEDVISPSDTENTVCATDPGGHSPQPQPSSPRSNTPDSVRDSIPCLRVKDRVIRMPPGYQAELVVQLLWVDGEPPQQITSLDLNSAYGLLAVGNCNGLAVVDYLQKTVILCMSTVELYGSADPFQRLTRSPRKNRQSTSDFCMRGLSNFYSDSKKRIRTSYQSLTELSDNQVSLDLERSKSPTSVPYPSSLHNDPRGTAEPVSNLRASHYSPVFYLLDNVKGPGRKLSLPTDLKTDLDHVNGHCTSPTSQPCVPGRPRVPGGPEGPRLSRRGPGRPPFRKAQSAACMEVSLPVSSLTEGYASRRAMLQQLHGVTMYSHDEHHTTTYSWNERESRENSFSRSRSSSVSSIDRDTKEAVTTLQFAESYGRKLDTLPTPCLWVGTSLGLVLLVPMSIPMDEQERMEEPITVAPTGTVLMLKGSVLRFGFLDCSGGLIQSPYELWRDLNAPEDPERPRRRKLVNFSPCSSQETASDGHLAVVCSERQAKVFLMPSQACLYVHNITESSFVLRADVVGVSNSVCLACFCANGHIMILSLPSLRPLLDVNYLPLTDMRIARTFCFTNGGQALYLSSPTEIQRITYSQEMCDNLQEMLGELFTPTETPEAQNRGFLKGFFGGNAQTFDREELFGEAAAGKASRSLAQHIPGQGSVEGVRVAAGGVVGDLARARIALDERGQRLGELEERTALMMSSAETFSKHAHELMLKCKDKKWYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJY3</accession>
    <name>A0A3B4EJY3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HPS4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037391923.1">
        <property type="nucleotide sequence ID" value="XM_037536026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJY3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036205"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039794.2">
        <property type="protein sequence ID" value="ENSPNAP00000036205.2"/>
        <property type="gene ID" value="ENSPNAG00000006265.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412052"/>
    <dbReference type="GeneTree" id="ENSGT00390000007349"/>
    <dbReference type="OMA" id="YACCSPV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031085">
        <property type="term" value="C:BLOC-3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903232">
        <property type="term" value="P:melanosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026091">
        <property type="entry name" value="HPS4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407:SF9">
        <property type="entry name" value="BLOC-3 COMPLEX MEMBER HPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME 4 PROTEIN LIGHT-EAR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="2">
        <location>
            <begin position="13"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="3">
        <location>
            <begin position="529"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036205.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="69282" checksum="23C515802F941441" modified="2024-11-27" version="2">MAETPLPETRRCIYFFLYDRSKVQEEGDLTRAGICYFYPEDTPLDQQELLCGQLAGVSRCVSELSSSPVRLLRLRKSKYAVHMRDNFLWVLSCVADIPDVSVCAFLDQLIDLFCFYNGPVRQSYQLHSQEELALRWACYLSNLQGGSTELHHIFSCLKTIDSTHIDPLLLLKAALILQACQRCPLVLAGCVLYRGRVVSTQMAPELTVKVMVHETETYSQDHRLRANGVTSGVSPPSSTVTTTVFLTPSELHTLHCPPVDNSCRSNGGPQPLARPRKSRLSRTLSDTPIADQDPAELDPAHFPPLSYQTLPSSPRSSLSDESCFSPSPSLVSTPLRSSLVSRSEGGTPPESRDRGLPNGTLPSKVTGGSVGGSSGVSVQEGERLDLESETKNPSENPEVCSLLNGHRSEQDDFSHQLSESLAKLGPVNDQSSGRSGQGGETERNQAKESKETGGEAARDCGAHSEASLQPMLLYQHRVRGLVLALLVEPSFNTHPSAKDEVYHSSLASLNGLEAHLRTTSPGTPGAQGPYTFAHYDCIQNTLTTNVCGRSAGSQDRPFVKATALLHSHFSHSDTLQEAIVRNAGCAVYGTRTAAQETYFLQQGASSRNSGIPNAQDSAFSLPSKARHRLLKHGVNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELX9</accession>
    <name>A0A3B4ELX9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PEST proteolytic signal-containing nuclear protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in cell cycle regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with UHRF2/NIRF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELX9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031851.2">
        <property type="protein sequence ID" value="ENSPNAP00000036783.2"/>
        <property type="gene ID" value="ENSPNAG00000027966.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010218"/>
    <dbReference type="OMA" id="KNMGRET"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029169">
        <property type="entry name" value="PCNP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16523">
        <property type="entry name" value="PEST PROTEOLYTIC SIGNAL-CONTAINING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16523:SF6">
        <property type="entry name" value="PEST PROTEOLYTIC SIGNAL-CONTAINING NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15473">
        <property type="entry name" value="PCNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="21"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036783.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="196" mass="20957" checksum="CE75C42D225D485F" modified="2024-11-27" version="2">ALSRAQGTNRGCLRQNAAWRASAHAHLRQPASAPSRPPSVRPPRFQLNGERAAVGRSGAARRSTGPGGGLGKRPASSSSSSRTEDECKKQHVPFKLAKPGFSAASQSLKKSPISIKLGASKPKEAAPAVPVKKPASVFDDDDSEPEEMPPEAKMRMKNIGRDTPTSAGPNSFNKGKQGFSDHQKLWERKLKSLTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EMX7</accession>
    <name>A0A3B4EMX7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037394373.1">
        <property type="nucleotide sequence ID" value="XM_037538476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EMX7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031250.2">
        <property type="protein sequence ID" value="ENSPNAP00000036526.2"/>
        <property type="gene ID" value="ENSPNAG00000004231.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439326"/>
    <dbReference type="GeneTree" id="ENSGT00390000011517"/>
    <dbReference type="OMA" id="WGSPMYV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056475">
        <property type="entry name" value="GBD_Hemicentin/VWA7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056861">
        <property type="entry name" value="HMCN1-like_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057615">
        <property type="entry name" value="Ig_VWA7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052577">
        <property type="entry name" value="VWA7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056862">
        <property type="entry name" value="VWA7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905">
        <property type="entry name" value="NG37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905:SF18">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING 10, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23560">
        <property type="entry name" value="GBD_Hemicentin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23619">
        <property type="entry name" value="Ig_VWA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25107">
        <property type="entry name" value="VWA7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25106">
        <property type="entry name" value="VWA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043859470" description="VWFA domain-containing protein" evidence="5">
        <location>
            <begin position="29"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="domain" description="VWA7 N-terminal" evidence="9">
        <location>
            <begin position="78"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Hemicentin-1-like von Willebrand factor A" evidence="8">
        <location>
            <begin position="311"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Hemicentin/VWA7 galactose-binding" evidence="6">
        <location>
            <begin position="508"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="VWA7 Ig-like" evidence="7">
        <location>
            <begin position="720"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036526.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="983" mass="104891" checksum="17E07A79878CF441" modified="2024-11-27" version="2">MMVTMRLPIRTSISFLLVVFLMQSQTWAFMPFAYPFFSSSSVTHKEISRVAILNTTVQLCRSQALSQGRDFVMPKTLTEGSVAAVCSSSASAKAFIRSIDKITENNAAVDLPWHYFFNPIYHFDDETFLRGRDLITSGVSIVKASVREQSYEAARAKLGEVLHTLQDFYSHSNWIELGNDTPYPNLIKPSSSIENIADSETCRSCDGSNCKGNILETVLRQKKLTSGYFNTFSFKKPKGKCSHGGALDLTSYTEPKGGINKDTLSSEHGDQHMDAVKVAIAATSDLLEDIRAAVGDSQFLRFMGLTQASVLCFVIDTTGSMSDDIAEVRRITSSIINSKTGSANQPSEYILVPFNDPDYGPLTRTNDPDVFKRKINALTADGGGDIPEMCLSGLQLALTGSPPQTEIFVFTDADAKDLSLKSTLLALIERTKSSVSFLLTNALSARRRRSSGGQQDGQQQFASRLTSPLNEVYQDLAQASGGQAIEVTKATLPQATSIIVDASSSSLVTVFQAVRNPAKVETFSFFVDSTLQNLTIYITGNSPSYTITSPSGVSQTSSQPSGVLGFIEKVGNFHTLHPNVSDQTGLWLISINSMQPYTVKVLGQSEVDFLFNFIELSEWPHPNYAVLNSRPTANSNITLLVSVVGWDSVRLTDVDLVEASSSKSYNGTLQEVGSGQYLVTISSIPTGEFVVRVVGESSSSKASNGFFQRQSSTQLQASSVMVTTQANGTMEPGKTFTLPFTVATNGSGGVFNIRVSNDRSFVMTDVPSSLILAHGGSANGTATLAVPGSTPSGSDVTVTIEAEAPGGADSNYAVLRLSVVAQVTDITPPVCEIVSVNANCSSNCSLSSWDLSANMTDGSGSGIQSVTVHQGNGTLNTSTVLDRGVNVTLASYSASCCSPQVELVVVDAVGNVGTCFRSLNAAVPQSTSATSTETVTNATTPATAATSGSTIQTSTNGAIGHLFLPVLFWLTVGTTSLYQYMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQS5</accession>
    <name>A0A3B4EQS5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">AKNA</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017570069.1">
        <property type="nucleotide sequence ID" value="XM_017714580.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570078.1">
        <property type="nucleotide sequence ID" value="XM_017714589.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570087.1">
        <property type="nucleotide sequence ID" value="XM_017714598.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570096.1">
        <property type="nucleotide sequence ID" value="XM_017714607.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000037496"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040936.2">
        <property type="protein sequence ID" value="ENSPNAP00000037496.1"/>
        <property type="gene ID" value="ENSPNAG00000004542.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437472"/>
    <dbReference type="CTD" id="80709"/>
    <dbReference type="GeneTree" id="ENSGT00940000154254"/>
    <dbReference type="OMA" id="WATEGNE"/>
    <dbReference type="OrthoDB" id="10035553at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001837">
        <property type="term" value="P:epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060234">
        <property type="term" value="P:neuroblast delamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021849">
        <property type="term" value="P:neuroblast division in subventricular zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052655">
        <property type="entry name" value="AKNA_Centrosome-Trans_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21510">
        <property type="entry name" value="AKNA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21510:SF15">
        <property type="entry name" value="MICROTUBULE ORGANIZATION PROTEIN AKNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="709"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="822"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="901"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1130"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1391"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="731"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="838"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="855"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="918"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="1006"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1044"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1069"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1158"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1179"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1211"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1258"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1422"/>
            <end position="1440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1469" mass="161585" checksum="4E89FBD9A3F8ED01" modified="2018-12-05" version="1">MERRRSGTRAGILLWTPTPGYSSSPPSPDGSGLSWDEGGEEDFESQMDENGIIGIRESLGEMDEEEEIVLEEEEDLLLDAGTPEPEEVPPPALEELSCHLSELLDSEPFSHHTGDDCPSPSNNDGDDSVALEDWSEDEDGQKLDEESLERLKIQEDSRPVGVKSMTIDDTEEVKVHREPLFCLREEMDPQFNCMESHDDNSNARKNNFTTSRSCRTTSGTLAGSQYCCDEEFILNERLHVSSSPLSSRNTPHSPVLPFLPQLSLEEQACNSGNGATETFPELAYTESKSHGTRLSCSPRSFQPVGCEEEKLEYGVIPQFGRSMRIKEDELERKVVGPYLTHHQLPIPSPQKISSALQESPQSSSPVQHAAKAQSSSSDSSPLSTPYHRRHRGHRPSKPSHTDLKDVRKGQLSHPLPDFSKVEPRVRFPKSGYKPPRSRRPPRKKDSKPDAPLVYRSPADIVREVLLSSTEGLSDAAAPGDPQGSLNSTAPTEFQCALQASTLVQQLQEDYNRLLTKYAEAENTIDRLRLEAKVGIYSDPPKASQPALPGVLQEGSKVMMLSFPQAQRAELSTNNVHPSQERINSEPSRGTSTLRLSVDHVSSMLPVSLAAIQLTEALSNHVQRFQLQVEEFEELLKSGKLRPYEQRQGLSQLAQGQSSLERTYLDARDQYQQLQKQAGGKSGCFDPDRELEGLIFNSGMRLEEMKERIEQSEQYGHVSDPGPNPPPHAYQPSASLNETDPQPESPISAVNAEGMVGMEVSSVSGDSAGDREENEEEILPSLLNPLHEKHQHVEKDFNSLMNHFQNFRELPKLLDRGPCEDAQDSLEFRDSSPQTARNDGIDLRQHGRTKDKGDSSKSFQPSPTAVSLFCPSSHSHGYNSMETLPEAPLPARILLGQQAGEMRPAGTRKPHSSLGKSANLEERASKEHDGVLRAAPQDGVISPETDSGFVGSESSRLTPAAHSPVQQRAAVRPSVTQEKSNIKPETVSVSGQLPRSLAHIHASPGHSGRGPGSTGRGGETERQTLPNPSTSSSPLRWTSGHLQPWPSSGISELEQQSDSAQSLSGEDVQQGDQYTKPSSQNGCYLRSPSSAMLYHHGDHLKAQSSVQLTNHHEALQSLQEEVDRLRERLKGSLRMTSPISPVRAPPSVLADCRNHKPSHTSTPLQATARSLEQRAASKRGPRENRRSEEEEQGERRRIPMHIPRRSASLPRFRPDLDITTDSDHAESEPKPQTSRHIPASLAAAHSSQRARAKVRQRTEPCTKSQQNSISADRSEEGDDEAEDSRGPAPLCMCCLARYGSRGFAPAPTRNVGSPSRTPHYSRHCPLCGARVSPPEHQNSAQRETKSVSSRHKYRGGVCLAGPLPPPVMGSVHVVQCVPVCPSVLYYSTPVAPSSNPKPLYLSRSEDRGYRSGVRGPHRRSRSVDTQHSLNSSLSRAVTAASSMKEVSRRMARSLASGLQHTSPIAQSYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IIP0</accession>
    <name>A0AAR2IIP0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calreticulin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasmic vesicle</location>
            <location evidence="15">Secretory vesicle</location>
            <location evidence="15">Cortical granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the calreticulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IIP0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044132.1">
        <property type="protein sequence ID" value="ENSPNAP00000039288.1"/>
        <property type="gene ID" value="ENSPNAG00000017127.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182915"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060473">
        <property type="term" value="C:cortical granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000002">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000122">
        <property type="entry name" value="Calreticulin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF3">
        <property type="entry name" value="CALRETICULIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00805">
        <property type="entry name" value="CALRETICULIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 17" id="KW-0143">Chaperone</keyword>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="184"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="196"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="94"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601580-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="428" mass="49455" checksum="30AE13D470529657" modified="2024-11-27" version="1">MFPPHIMIVQNTQMQISLKHGWKSRWAESKHKSDYGQWKLSAGKFYGDAELDKGLQTSQDARFYAMSSRFEPFSNEGKDLVIQFTVKHEQKIDCGGGYVKVFPADLDQAEMHGDSQYYVMFGPDICGYSTKKVHVIFNYKGQNHLIKKEIRCKDDEHTHLYTLILHPDQTYEVKINNEKVESGSLEEDWDFLPPKKIKDPEAKKPEDWDDRAKIDDPEDTKPEDWDKPENIPDPDAKKPDDWDEDMDGEWEPPMIPNPEYKGEWKPKQIDNPNYKGAWVHPEIDNPEHTPDDSIYKYDNIGVIGLDLWQVKSGTIFDNFLISDDVKEAEEFGKETWGATKGAVLGLHGPPNGCASDQRNPQMREVSPLYYTSLMSFQCVMVLFLITSILFHLNGTAAPFKVERDGCDCREIQRRTWRISDFSFISLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IMV9</accession>
    <name>A0AAR2IMV9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KCNIP2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IMV9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052036.1">
        <property type="protein sequence ID" value="ENSPNAP00000041018.1"/>
        <property type="gene ID" value="ENSPNAG00000005630.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157798"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000043">
        <property type="entry name" value="Kv channel-interacting protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF65">
        <property type="entry name" value="KV CHANNEL-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="7" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="140"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="234" mass="27137" checksum="9C714F90E6033E70" modified="2024-11-27" version="1">MGLHVMLEVLVPAREQMGGIYALVVFALFPLSYWLFGKDSIEDDFELSTVCHRPEGLEKLQEQTKFTKKELQVLYRGFKNECPSGVVNEDTFKVIYSQFFPQGDSSTYAHFLFEAFDTNKNGSVSFEDFVIGLSIILRGTINDRLNWAFNLYDLNKDGCITKEEMLDIMKSIYDMMGKYTYPCMQEDAPREHVETFFQKMDRNNDGVVTIDEFIESCQKDENIMQSMQLFDNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IP31</accession>
    <name>A0AAR2IP31_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IP31"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045005.1">
        <property type="protein sequence ID" value="ENSPNAP00000039802.1"/>
        <property type="gene ID" value="ENSPNAG00000037098.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162437"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036128">
        <property type="term" value="C:CatSper complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060296">
        <property type="term" value="P:regulation of cilium beat frequency involved in ciliary motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028746">
        <property type="entry name" value="CatSper1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053873">
        <property type="entry name" value="CATSPERG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47193">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47193:SF1">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22846">
        <property type="entry name" value="CATSPERG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERG C-terminal" evidence="7">
        <location>
            <begin position="31"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="170"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49697" checksum="B49E5C630A2D08CB" modified="2024-11-27" version="1">MFKVVGGGRTRGSVQNYSREEILRFNSLTDRWLCQRNSPCGDLTPRNLLDPDFFFIIEVSNKETASSPFCEHNLRFELHVHGSPLDPVRGLFFLQMTMTAVHIILICTAGHTCWRLRRKSLHTKKRDGLDEQEGHKQLKARDVVVKVGWTLRSCFLQLDHIIFRIIQKPMLDHFMMLVVLLDTSTLMAQTFPGVNVRTSWCLSAVDSCLLVFFMMEAVFKIQVRGRTYFRNPWNDLDFFIVLMCLVDFTLSLVQSAGGFSSRQASTLAPGAQDFERSPSVQSFTSTQSHPLLQGSPGHPFNLPSVVRSMSSIIVLMFLFLFIFAVIFREMFNESDPEHFGNIFRTIFTLFQTLTLDDWSLIYMTSRDNGAPHIIYFLSLYILVELFTFLNLFIAVLVDNFQLTIRRKRHCKSQKVERVKRLQWRIIQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ISP6</accession>
    <name>A0AAR2ISP6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">KHSRP</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2ISP6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086329.1">
        <property type="protein sequence ID" value="ENSPNAP00000042735.1"/>
        <property type="gene ID" value="ENSPNAG00000012026.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156051"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22479">
        <property type="entry name" value="KH-I_FUBP2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22482">
        <property type="entry name" value="KH-I_FUBP2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22485">
        <property type="entry name" value="KH-I_FUBP2_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22488">
        <property type="entry name" value="KH-I_FUBP2_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000007">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000010">
        <property type="entry name" value="far upstream element-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015096">
        <property type="entry name" value="FUBP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047372">
        <property type="entry name" value="KH-I_FUBP2_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047369">
        <property type="entry name" value="KH-I_FUBP2_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047370">
        <property type="entry name" value="KH-I_FUBP2_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047371">
        <property type="entry name" value="KH-I_FUBP2_rpt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09005">
        <property type="entry name" value="FUBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="110"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="196"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="288"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="395"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="363"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="475"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="559"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="559"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="663" mass="68629" checksum="534DA17D50441855" modified="2024-11-27" version="1">MSEYSAVPPPGAPGAAAALGAGGIKKDAFADAVQRARQIAAKIGGDVAAPPMSNNGGAESYPFAAQKRSLEDGDQPDSKRMASQSDRDNATALSIGAQLAALSQQSVRPSSVTEDYRVPDGMVGLIIGRGGEQINKIQQESGCKVQIAPDSGGLPERSVSITGSPEAIQKAKMLLDDIVSRGRGTPPTAFHETNGSGHMQEMIIPAGKAGLVIGKGGETIKQLQERAGVKMILIQDASQGPNMDKPLRIIGEPYKVQQAREMVQEILRERDHPGFGDRNDYGSRMGGGGGGIEVPVPRHSVGVVIGRNGEMIKKIQNDAGVRIQFKPDDGNGPDKIAHIMGPPDCCEHAASIINDLLQSIRVREEGGGGPPGPPGTGMPPGGRGRGRGPGNWGPPGGEMTFSIPAHKCGLVIGRGGENVKAINQQTGAFVEISRQPPPNGDPNFKLFTIRGTPQQIDHAKQLIEDKIEGPLCPVGPGLGGPGPAGPMGPYNPNPYQPGPPGAPPHGGPPGAHQYPPQGWGNTYQQWQPPAPHDPSKAAADHSAAWAAYYAQYYQQPAGGAMPGQAPSGQPAAPAPADQSQAAQSTGQPDYTKAWEEYYKKMAQQGGGAAPAAGAAPAAAGAAAGQTDYSAAWAEYYRQHAAYYGQTAQTAGQPAAAPQGQQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWW6</accession>
    <name>A0AAR2IWW6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc-binding protein A33-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TRIM4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWW6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068181.1">
        <property type="protein sequence ID" value="ENSPNAP00000042086.1"/>
        <property type="gene ID" value="ENSPNAG00000030240.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193390"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12893">
        <property type="entry name" value="SPRY_PRY_TRIM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="84"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="272"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="161"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="54374" checksum="696D4411CC65B40F" modified="2024-11-27" version="1">MASKFSEEDFSCPVCREIFRDPVILRCSHSVCKDCLQQFWKIKGSRECPVCRRKSPVEKPPINLALKNLCETFLQESSPRASAGSGTRCIPHSEKLRLFCLDDLQPVCLVCRDSKIHTNHKFLPLQEAAVDYKKELQAALKPLEEKLKIFKECKLSWDQTAEHIKTQARRTERRIKEEFEKLHQFLRDEEAARLTALREEEERKSRAMKEKIEKMSREISSLSDAVRAVEEQMAAEDVSFLQNYKATLKRTQCSLQDPERLSGELLHVAKHLANLKFTVWEKMQKIMQFAPVILDPNTAHPKLVLSDDLTSVRFSDEKQQLPDNPERFDKLVCVLGSEGFNSGTHCWDVEVGDKLWILGVMTESAERKGEIFSRSGVWSLWYLNGQYAVGSSPQPGTPLSVAQNLQKIRVQLDWERGMLSFSDGLTNRDLHTLSHRFTERVFPLFMNVCKVSPKKIIPVLKEPTQSLLQLSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IXZ6</accession>
    <name>A0AAR2IXZ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IXZ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059891.1">
        <property type="protein sequence ID" value="ENSPNAP00000042471.1"/>
        <property type="gene ID" value="ENSPNAG00000032070.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cystatin" evidence="4">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="52" mass="5762" checksum="8D3B8C3CC206DFE3" modified="2024-11-27" version="1">KVLSGVAQPGTCSYIKNIEKQTGKQFAVFETLTFRTQIVAGKNYNIKVQIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0T3</accession>
    <name>A0AAR2J0T3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pseudouridine-5'-phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Haloacid dehalogenase-like hydrolase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Haloacid dehalogenase-like hydrolase domain-containing protein 1A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Pseudouridine-5'-monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PUDP</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Dephosphorylates pseudouridine 5'-phosphate, a potential intermediate in rRNA degradation. Pseudouridine is then excreted intact in urine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>psi-UMP + H2O = pseudouridine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10944"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17802"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58380"/>
            <dbReference type="EC" id="3.1.3.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.96" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0T3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072801.1">
        <property type="protein sequence ID" value="ENSPNAP00000043431.1"/>
        <property type="gene ID" value="ENSPNAG00000032390.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108428251"/>
    <dbReference type="GeneTree" id="ENSGT00390000014753"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990738">
        <property type="term" value="F:pseudouridine 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07529">
        <property type="entry name" value="HAD_AtGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000055">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045228">
        <property type="entry name" value="Gpp1/Gpp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="226" mass="25305" checksum="C771D71C55322035" modified="2024-11-27" version="1">MTYKPVTHVIFDMDGLLLDTEKLYTVSFQEICDRYGKTYTWDVKSSVMGKKALDAARIIRDSLELPMSPEELLEESRKIQERIFPNAALMPGVEKLITHLHKDNIPIAVATSSASLTFEMKTSRHKDFFSLFKHIVLGDDPEVKNGKPQPDAFQVCASRFDPPASPEQCLVFEDAPNGVKAGLAAGMQVVMIPDQNLDHSLTQEATLVLDSMEDFRPELFGLPAYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J2B7</accession>
    <name>A0AAR2J2B7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PRELID3A</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017557495.1">
        <property type="nucleotide sequence ID" value="XM_017702006.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J2B7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083656.1">
        <property type="protein sequence ID" value="ENSPNAP00000043961.1"/>
        <property type="gene ID" value="ENSPNAG00000030304.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429928"/>
    <dbReference type="CTD" id="10650"/>
    <dbReference type="GeneTree" id="ENSGT00950000182810"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="172" mass="19495" checksum="FE7C49E4CC60A2CA" modified="2024-11-27" version="1">MKIWSTEHIFSYPWETVIKAAMRKYPNPMNPSVVGVDVLDRNLDKNGRLHSHRLLSTEWGLPGVVRAILGTNRTITYVKEHSIVDPQEKKMELCSTNITLTNLVSVDERLVYRPHPENPDVTILTQEAIITVKGVSLSSYLEGLMAISMSANARKGWDAIEWIIKNSECEMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J2X5</accession>
    <name>A0AAR2J2X5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J2X5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051485.1">
        <property type="protein sequence ID" value="ENSPNAP00000046368.1"/>
        <property type="gene ID" value="ENSPNAG00000027998.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163923"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000087">
        <property type="entry name" value="Counting factor associated protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043490499" description="Peptidase C1A papain C-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="7">
        <location>
            <begin position="342"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="561" mass="62266" checksum="804D3271666B6042" modified="2024-11-27" version="1">RPTMWPFLAVAALLGCASVLEATPLTGRTLPDFGNTYHVKGVISLPYAEIKEPFKGWYDLKGKRSRIDYYNGQVSTFQLGTAMPQGVSYKITPVTTETEFNAMKCFQVNGTKEDPVLPQAALPNVQGFQFEQMDYHGGVLCEVWKNITTIGHKKNTYRLWVTRPEGGPSVATPHHYEMMGYNTLLGSHYDKYLIDYSDFSPKTDPKDFNLPDGLDCGPFPGPGAEHRLLANPIQDLIHTSPVGHAHRLFGHFRDKYKRDYETEEEHEITNNFVSFPHAKPCLLLFSLRYVHSMNRAGLSFSLSVNHLADHSQAELAVMRGRILKKTPNNAQPFPTELRSIAAPDSLDWRLYGAVTPVKDQAVCGSCWSFATTGALEGALFLKTGEQVVLSQQMLVDCTWGFGNNGCDGGEEWRAYEWIMKHGGISTAESYGGYTGMNGLCHYNSSSLTAHVRSYTNVTSGDMEALKAALFKNGPTAVSIDASHRSFTFYSNGVYYEPACKNGIDDLDHAVLAVGYGVLDNQPYWLVKNSWSTYWGNDGYVLMSMKDNNCGVATDATYVTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J385</accession>
    <name>A0AAR2J385_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Macrophage-capping protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Actin regulatory protein CAP-G</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Interacts with NUP62. Interacts with NUTF2 and RAN; involved in CAPG nuclear import.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J385"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048413.1">
        <property type="protein sequence ID" value="ENSPNAP00000044782.1"/>
        <property type="gene ID" value="ENSPNAG00000008567.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164612"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000004">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000005">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000040">
        <property type="entry name" value="macrophage-capping protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000037">
        <property type="entry name" value="macrophage-capping protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF27">
        <property type="entry name" value="SCINDERIN LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0117">Actin capping</keyword>
    <keyword evidence="13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="57"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="171"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="287"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="430"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="549"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="651"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="749" mass="82738" checksum="09705889F3991B84" modified="2024-11-27" version="1">MSYFAFSSLPIVALFHEPRLPSRPRCLIPIMVYHKEFQTAGKEPGLQVWRVEKMDLKPVPKQLYGNFYTGDAYILLYTTPAPSYAIHMWLGSECSQDESGAAAIFATQLDDFLGGGPVQFREVQNNESLTFLGYFKSGVKYKQGGVASGFQHVVTNDMNVKRLLHVKGRRVIRATEVPLSWCSFNRGDCFIIDLGKDICQWCGSECNRFERLKASQVAIDIRDNERNGRARLQMVEDGSEPQAVTDVLGPRPNIPPGSPDDDAVERANKKGTLYMVSDAAGSMKASVVAQSSPFKQEMLSPSECYILDNGIDGKIFVWKGPTANAAERKAAMKAAEQFVKEKNYPKNTQIQVMPAGGETTLFKQFFSNWRDKDQTTGPGKAYTIGRIAQVEKIPFNASSLHSNKAMAAQHGMVDDGSGKVQVWRVEGGDKVLVDPSSYGQFYGGDCYLILYSYHQGGREQHIIYTWQGLKCTQDELAASAFLTVKLDDSMGGAPVQVRVTQGQEPPHLMSLFKGKPMIVFLGGTSRKGGQSRTGSTRLFHIRQSSTRATRAVEVEPTASNLNTNDVFVLKSPDSLFLWKGVGATEEELAAAKFVCSFLGGSVTEVAEGKEPAGFWSALGGKKEYQTSSSLQKTVKPPRLFGCSNKTGRLIVEEVPGDFTQADLATDDIMLLDTWNQIFVWVGNEANEVEKSGSAKIAKEYVDSDPAGRRGTPITTIKQGFEPPTFTGWFQAWDAKMWDTDPLERIRARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J528</accession>
    <name>A0AAR2J528_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J528"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048872.1">
        <property type="protein sequence ID" value="ENSPNAP00000044926.1"/>
        <property type="gene ID" value="ENSPNAG00000033864.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01080000257344"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001495">
        <property type="entry name" value="Si:dkey-234i14.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="205" mass="22762" checksum="A969E55AAAAFAB1B" modified="2024-11-27" version="1">FLSGCSVQVTVTQTPAVKTVSPGDSVTISCRTSTSVYGGDRLAWYQQKPGQAPKLLIYYATSRQSGIPDRFSGCGSGSDFTLSISNVQTEDAGDYYCQSAHYISSYVFPQCLVGVQKPPLSPAAQRLHCCSWTLLQVLRGRMDTHNNTLCGNQDFRWSLFFCSLDWKCFVILGVYFLISTGLELAVEFSSSLIYSKVYYKDLDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JAM2</accession>
    <name>A0AAR2JAM2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TRIP11</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000075223.1">
        <property type="protein sequence ID" value="ENSPNAP00000047387.1"/>
        <property type="gene ID" value="ENSPNAG00000036850.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183078"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051841">
        <property type="entry name" value="MT-Golgi_org_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18902:SF24">
        <property type="entry name" value="NUCLEAR MITOTIC APPARATUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18902">
        <property type="entry name" value="NUCLEAR MITOTIC APPARATUS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1624"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1843"/>
            <end position="1879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1895"/>
            <end position="1924"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="57"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="213"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="662"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="821"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="980"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1192"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1637"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1845"/>
            <end position="1876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1903"/>
            <end position="1917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1940" mass="220998" checksum="E57870D53A9FF73C" modified="2024-11-27" version="1">SSSASSYSDEGSPFFTRSRSPRVSFLELNTVASSSFVSVSVLSSPIQEIINTPQVQLKRLRKELAREGDVRDELERELSKHISTISEKEGQISQLHHRLQRLLRDQEEFEKEHKSALLELQQKNEGLLKRVHEVLKQCQDLKTDNTQKDRRIDELMEENGTLAAQECLSEQIQILQGKISVLEDELSRVSVQLQEKGEVLGPVMEVGIGLILREKLKQELADLSLKLSQLEETISCLQKEKMDVEMLLLEERSSFERETLRLQGLISDLQQAINNIRAEREAQEQTARQKQELLTTQIAALETDLNRLQQLEVQLTAEIAITAELRQQRVELEAKVASLEDTISMLRSKCQGMEVESEAQQERLNIVRADLQKAQSSLVEYEEKLADHQKVVDENTNLRARISALDDTVALLQNEIDTEKKRGDEVIAEKEQHKALMEEKFQKQEERAHEMFVELETLILAASLTEERDRAQLQLVVVRKAKDEAELELQRIIAEHESKISQLKTGLKNIQDGHQKELEALQTEKEQIFSANQSLQNECQKMDILINEKEHLCSVNQSLQNECQSLQADIKRQQEELNVLKADLQNTKEGHKTEINILINEKEHLSSANQSLQIECQNLQAEIKGQQDALNTLKTDLQNTQEGHKKDLETFMKEKENRCAVNQSLQNECQTLQAEIKGQQEELNMLKADLQNTKEGHKTEMDILINEKEHLCSVNQSLQNECQSLQADIKRQQEELNVLKADLQNTKEGHKTEINILINEKEHLSSANQSLQIECQNLQTEIKGQQDALNTLKTDLQNTQEGHKKDLETFMKEKENRCAVNQSLQNECQTLQAEIKGQQEELNVLKADLQNTKEGHKTEINILINEKEHLSSANQSLQIECQNLQTEIKGQQDALNTLKTDLQNTQEGHKTEIDILINEKEHLSSVNQSLQNESKNLQAEIKGQQDALNTLKTDLQNTQEGHKKDLETFMKEKENRCAVNQSLQNECQTLQAEIKGQQEELNMLKADLQNTKEGHKTEMDILINEKEHLCSVNQSLQNECQSLQADIKRQQEELNVLKADLQNTKEGHKTEINILINEKEHLSSANQSLQIECQNLQAEIKGQQDALNTLKTDLQNTQEGHKTEIDILINEKEHLSSVNQSLQNESKNLQAEIKGQQDALNTLKTDLQNTQEGHKKDLETFMKEKENRCAVNQSLQNECQTLQAEIKGQQEELNVLKADLQNTKERHKTEMDILINDKEHLSSVNQSLQNECQSLQADIKRQREELNVLKADLQSTKDGYQQELDTLKKEKDHVSSVNQSVQSECQSLRAENKGKQETLKTLKADLVNAQNGYQQKLDTLQKEQERLSSVNHSLQRDCQSLQAEIKELQDMQDGYQKAVEILQKEKEQLSSVNQSVQSEHQSLQAEIKGKQEELNVLKADLQNTQDEYHRELETFKKEKEQLSSVNQSLQSESQSLRVELSKLQKTQEGYQKDMETLQKEMEQVSSENQSLLNECQNLQAENKGQQEVLKTLKAELQNTQNGYQQELETLRKEQENLSSDNQSLQSECQSLQKEIKGQQEELNVLKSQWRCKTPALTAWSSIWKILSMLEGNSLCPFCKCLKYHSHLEPYVQPVSHFWSLASKPAVPGESSTPLVRSSERLASKRRAQERGSLETLYFTPMNPRNKNHDAANHKNKLESSITSLGDLTLDSAKKLTSSVRRRRTTQVINITMTKKTPGGTGGEAEDSFYSLHTTQSQPNLASQKTRPVSMEIFEEPGAASSSDKLLSLPGYRRSNVHGNYEPEHVADDWMRIAELQSRNKACLPHLKSSYPLESRPSLGFSVLPITDEDVRFGDPEETIRRASMVPSQLTESLSSHRLSLAPPSSNRDHPTSQPQRYSMLPGQISAGTAKKLASCFPRPATPKNRNLRSAASNSQNRPPSPVSRAESGVHFCVYIVMEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JBB0</accession>
    <name>A0AAR2JBB0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JBB0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084015.1">
        <property type="protein sequence ID" value="ENSPNAP00000047627.1"/>
        <property type="gene ID" value="ENSPNAG00000033119.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000044642"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000063">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF9">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="186"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="375" mass="42788" checksum="6D38F8BB9DFE6953" modified="2024-11-27" version="1">MRLMRYKPEAVYVPGKTLVVADALSRSPQIHTEEKTDTHSVVEHYIATVMQGIPASQSRMDSIKAATTTDCELQTVIRYIRNGWPEYRDKVPLDVRAYVKVKNELSETNGLVIRGCRIVIPKSLRAEILKKIHDGHQGLTKCRDRANSSVWWLGLSAELKETVMQCHTCQELRRTQQKEPLISTPLPGRPWKRIALDLCEYNKNDYLIVSDYYSRFLEILHLPSTTSAQVIQRLKTTFARFGIPDEVVSDNGPQFSSAEFKELAQQMDFKHLTSSPHHPQGNGHAERAVQTAKRILKQEDPLLALMCHRSTPCTTTGVSPAELLMGRKIRTTLPILEKNLQPKWPNRKAVMQKDAVEKAKQAFYYNRRHGARLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JCK1</accession>
    <name>A0AAR2JCK1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60 kDa heat shock protein, mitochondrial</fullName>
            <ecNumber evidence="3">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Chaperonin 60</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Heat shock protein 60</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JCK1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044725.1">
        <property type="protein sequence ID" value="ENSPNAP00000048057.1"/>
        <property type="gene ID" value="ENSPNAG00000000839.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005727"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000019">
        <property type="entry name" value="60 kDa heat shock mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000031">
        <property type="entry name" value="60 kDa heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000026">
        <property type="entry name" value="Chaperonin 60 subunit alpha 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000018">
        <property type="entry name" value="Heat shock protein 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="597" mass="63681" checksum="20F8C1DA298B3F5D" modified="2024-11-27" version="1">LVKLPLRKSLHKIFMNTLPEHETLCSDIVMRQMRPVCRALAPHLTRTYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQTWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARAIAKEGFDTISKGANPVEIRRGVMFAVETIINELKKLSKPVTTPEEIAQVATISANGDTEVGKIISDAMKKVGRKGVITVKDGKTLHDELEIIEGLKFDRGYISPYFINTTKGQKCEFQDAYLLLSEKKISSVQSIVPALEIANQHRKPLVIVAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLQDMAIATGGVVFGDEATGLAVEDIQAHDFGRVGEVVVTKDDTMLLKGQGDAAAVEKRVAEIAEQLENTTSDYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVPGGGCALLRCIPALDTLKPANDDQKVGIDIIRRALRIPAMTIAKNAGVEGSLVVEKILQGDAEVGYDALHGEYVNMVERGIIDPTKVVRTALLDAAGVASLLSTAEAVVTELPKEEKEMPGGMGGMGGMGGMGGGMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JEE2</accession>
    <name>A0AAR2JEE2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin-conjugating enzyme E2 H</fullName>
            <ecNumber evidence="9">2.3.2.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">(E3-independent) E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquitin carrier protein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin-protein ligase H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">UBE2H</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with MAEA and WDR26, components of the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.24" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017566180.1">
        <property type="nucleotide sequence ID" value="XM_017710691.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JEE2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065495.1">
        <property type="protein sequence ID" value="ENSPNAP00000050398.1"/>
        <property type="gene ID" value="ENSPNAG00000036267.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108435107"/>
    <dbReference type="CTD" id="7328"/>
    <dbReference type="GeneTree" id="ENSGT00390000004852"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000078">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4 18" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="20">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="152"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="17">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="183" mass="20655" checksum="D4C81BF914DE3F8D" modified="2024-11-27" version="1">MSSPSPGKRRMDTDVVKLIESKHEVTILSGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGAGDSSSESSMSDFSEDEAQDMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JNB2</accession>
    <name>A0AAR2JNB2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Lactoylglutathione lyase</fullName>
            <ecNumber evidence="3 14">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glyoxalase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GLO1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-S-lactoylglutathione = methylglyoxal + glutathione</text>
            <dbReference type="Rhea" id="RHEA:19069"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="4.4.1.5"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:19071"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the glyoxalase I family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="3 14"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JNB2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000088028.1">
        <property type="protein sequence ID" value="ENSPNAP00000051684.1"/>
        <property type="gene ID" value="ENSPNAG00000016646.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009312"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000011">
        <property type="entry name" value="Lactoylglutathione lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00935">
        <property type="entry name" value="GLYOXALASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="8 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="15">
        <location>
            <begin position="26"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="152"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="192" mass="21858" checksum="B776F253D781D2C0" modified="2024-11-27" version="1">MADQGLSDEAAAAACKDGNPITKDFMMQQTMLRVKDPVKSLDFYTRILGMTLLQKFDFPSMRFSLYFLGYEDKKEIPAETKERTAWTFSRRATIELTHNWGSETDDSQSYHNGNSDPRGFGHIGIAVPDVYAACKLFEEQGVTFVKKPDDGKMKGLAFIQDPDGYWIEILSPNNMCNKTLTYMLFSFKKKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JQK5</accession>
    <name>A0AAR2JQK5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CRYGS</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JQK5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070072.1">
        <property type="protein sequence ID" value="ENSPNAP00000052464.1"/>
        <property type="gene ID" value="ENSPNAG00000033666.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163322"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF129">
        <property type="entry name" value="CRYSTALLIN, GAMMA M6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="13"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="52"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="142"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="186" mass="21688" checksum="8FB9F2D99CBEE5E2" modified="2024-11-27" version="1">MSASFDIPLSPCCQITFFEEKKFQGRCYETNGDCANLLSFFSRCNSIRVESGCWVLYEKPNYKGYQYILSPGDYADHQQWMGFSESIGSCRSIKNVYGNTWRLRLYEKPDFGGQMVECTEDCPSVHEAFKLREVFSCVVLQGAWVFYELPGYRGRQYFVERGDYRKYSDWTAPNPVLGSFRRITEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JW15</accession>
    <name>A0AAR2JW15_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JW15"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049227.1">
        <property type="protein sequence ID" value="ENSPNAP00000057531.1"/>
        <property type="gene ID" value="ENSPNAG00000037885.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000055816.1">
        <property type="protein sequence ID" value="ENSPNAP00000054041.1"/>
        <property type="gene ID" value="ENSPNAG00000032244.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286933"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF134">
        <property type="entry name" value="TRANSPOSABLE ELEMENT TC1 TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13384">
        <property type="entry name" value="HTH_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="145"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38118" checksum="A131BC14099F90D4" modified="2024-11-27" version="1">MGKQRDLSTGIVAQIQALRQQGLTQTKIAEQLGISQSSVCKALKKNCSKRTNCSGVRKTSARDNKQLRKIAVSNRFKSTSELTDLWNKQTGADVSRSTTYRRLRELGFKSRVPAVKPMLNKKQMEKRLKWAKEHSKWTAEDWQKVVFSDESRFCISFGDQGPRVWRRGGETYNHECVKRSVKFPQSVMVWGCMSARGVGKLCFLKKTVNAAVYQDILETFLIPTVEEQFGEEDFIFQQDLAPAHAAKSTKDWFTKKQLEVLAWPANSPDLNVIENLWAIVKRKIRDRKPTTLDQLKQNIATAWEAVSAETCDKLVKSMPRILQAVIQAKGAATKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JYR8</accession>
    <name>A0AAR2JYR8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Very-long-chain enoyl-CoA reductase</fullName>
            <ecNumber evidence="6">1.3.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Synaptic glycoprotein SC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Trans-2,3-enoyl-CoA reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Involved in both the production of very long-chain fatty acids for sphingolipid synthesis and the degradation of the sphingosine moiety in sphingolipids through the sphingosine 1-phosphate metabolic pathway. Catalyzes the last of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme reduces the trans-2,3-enoyl-CoA fatty acid intermediate to an acyl-CoA that can be further elongated by entering a new cycle of elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Catalyzes the saturation step of the sphingosine 1-phosphate metabolic pathway, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2E)-hexadecenoyl-CoA + NADPH + H(+) = hexadecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:36143"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:36144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(2E,7Z,10Z,13Z,16Z)-docosapentaenoyl-CoA + NADPH + H(+) = (7Z,10Z,13Z,16Z)-docosatetraenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:39331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:73856"/>
            <dbReference type="ChEBI" id="CHEBI:76416"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:39332"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(2E,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + NADPH + H(+) = (7Z,10Z,13Z,16Z,19Z)-docosapentaenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:39467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:73870"/>
            <dbReference type="ChEBI" id="CHEBI:76461"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:39468"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(2E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + NADPH + H(+) = (8Z,11Z,14Z)-eicosatrienoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:39319"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:74264"/>
            <dbReference type="ChEBI" id="CHEBI:76412"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:39320"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a very-long-chain 2,3-saturated fatty acyl-CoA + NADP(+) = a very-long-chain (2E)-enoyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14473"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:83724"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="EC" id="1.3.1.93"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:14475"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>octadecanoyl-CoA + NADP(+) = (2E)-octadecenoyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35351"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:71412"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:35353"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.93" evidence="6"/>
    <dbReference type="RefSeq" id="XP_037387073.1">
        <property type="nucleotide sequence ID" value="XM_037531176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JYR8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062851.1">
        <property type="protein sequence ID" value="ENSPNAP00000054991.1"/>
        <property type="gene ID" value="ENSPNAG00000031493.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108415987"/>
    <dbReference type="CTD" id="450002"/>
    <dbReference type="GeneTree" id="ENSGT00950000182886"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102758">
        <property type="term" value="F:very-long-chain enoyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000083">
        <property type="entry name" value="Trans-2,3-enoyl-CoA reductase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049127">
        <property type="entry name" value="TECR-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF59">
        <property type="entry name" value="STEROID 5-ALPHA REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21696">
        <property type="entry name" value="TECR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 29" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="TECR-like N-terminal" evidence="31">
        <location>
            <begin position="38"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="30">
        <location>
            <begin position="189"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00050808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00057553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00072713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00078575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00081803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF21696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="342" mass="39720" checksum="5A025BDF57F5D246" modified="2024-11-27" version="1">MECLELKDLFTVAQFIKARCDHGLKAGGPANFKNYVFFEVEILDPKSKAQLCYLDKVEPNATVGEIKNLLHRLYPKWYPSRQALKLHPEGKALKDEEVLEDLPVGTTATMFLQDLGPQLSWTMVFLAESIGPLFIYLLFYFRLPYIYSPEDDYTKSPHAVVRLACMCHSLHYIKRLVETIFVHRFSHGTLPVQTIMLNCLYYWGFAAWLAYYINHPLYTTPTYGKLQICCALFTFLVCECGNFSIHLALNRIRCNGARPRQIPYPTKNPFTWLFFFVSCPNYTYEVGAWLSFAVMTQCVPVVLFTFVGFAQMTIWARRKHRTYLEELKDYPELRSAIVPLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K3M5</accession>
    <name>A0AAR2K3M5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Rac GTPase-activating protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Male germ cell RacGap</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">RACGAP1</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Secretory vesicle</location>
            <location evidence="4">Acrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K3M5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082415.1">
        <property type="protein sequence ID" value="ENSPNAP00000057077.1"/>
        <property type="gene ID" value="ENSPNAG00000022256.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154610"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097149">
        <property type="term" value="C:centralspindlin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20821">
        <property type="entry name" value="C1_MgcRacGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04382">
        <property type="entry name" value="RhoGAP_MgcRacGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000034">
        <property type="entry name" value="Rac GTPase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000033">
        <property type="entry name" value="Rac GTPase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46199">
        <property type="entry name" value="RAC GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46199:SF5">
        <property type="entry name" value="RAC GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="21" id="KW-0007">Acetylation</keyword>
    <keyword evidence="28" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="14" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22 32" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="29" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="26" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="17" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="23" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="24" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="25" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="19" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <keyword evidence="16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="34">
        <location>
            <begin position="301"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="35">
        <location>
            <begin position="364"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="180"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="32">
        <location>
            <begin position="58"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="33">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="33">
        <location>
            <begin position="593"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00067896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00075869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="646" mass="72609" checksum="2C17A33B9DA493FF" modified="2024-11-27" version="1">METAVMNLLGVFENLRAHVDILNESVEPQFIQMALNFEDCRRKWLRVEHELASCKEVLAKAETERGALEVKLKHARNQVDVEIRRRQKAEADCEKLDRQIQLIRDLLVTEGSSSSIQLNDEQRSALAFLNARSQAPANLNSSRRLTTIDESASILSDISYDKTDDSLDWDSSVVRTVRLKKRQKRRSSRNHTDGPPAAAKRSRSTGRTSEKGNESLVAKTTVTVPKNGGAIEAVTTVEAVPYWTRSRRKTGEFQCKYAAMEWNTADTDSIQSVDVFKRPNHVERESKAEPNTPQSNGSVRLHEFVSKTVIKPESCVPCGKRIKFGKISLKCRDCRVVAHPECRERCPLPCIPNMAGTPVKIGEGTLADYVSVTSPMIPPLVVHCVNEIEQRGLHEIGLYRLSGADRVVKELKEKFLRGKTVPLLSKVEDIHAITGLLKDFLRNLKEPLLTFRLNRNFMEAAEVADEDNSIALMYQSISDLPQPNRDTLAFLIIHLQRVARSPDTKMDEINLSRVFGPTIVGHAVPDPDPMTILQDTKRQPKVVERLLGLPIEYWSRFMMVEQDQAHNDHVIIENTNVYATPDHKVSIFGPLTTPEQQMSKTPSSSSLSQRMRNATLNAITPKFGSKSKSTVGFSRQGNFFASPLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K5D3</accession>
    <name>A0AAR2K5D3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K5D3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067598.1">
        <property type="protein sequence ID" value="ENSPNAP00000059470.1"/>
        <property type="gene ID" value="ENSPNAG00000032076.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156937"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044291">
        <property type="term" value="C:cell-cell contact zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098636">
        <property type="term" value="C:protein complex involved in cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000342">
        <property type="entry name" value="Junctional adhesion molecule A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042974">
        <property type="entry name" value="JAM-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44598:SF3">
        <property type="entry name" value="JUNCTIONAL ADHESION MOLECULE 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44598">
        <property type="entry name" value="JUNCTIONAL ADHESION MOLECULE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="19"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="152"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="323" mass="36244" checksum="E38BB57BDA18EC5B" modified="2024-11-27" version="1">MLQDVFRVGSEVFKRLQRPDVEESIDANSLLLVFITGCVSVLAVVLKTSNKTPWANEFESIELSCLIESISTVNPRIEWKKIKNGEQPSYVYYEKKISGDLENRARLREPATLVIVNATRSDTAFYRCEVTAPSDHKTFDEILIDLRIRVKPVVPTCSVPKSVPVGKSAELSCVEHEGFPRSQYQWFRNKEEIPEDPKSSPKFFNSSYVLNGESGTLKFSAVRKEDAGQYYCRAKNEAGVSECAPQMMEVYDINIAGIVLGVLVVVVVLLCITVGICCAYRRGYFASQKQMGSNYKTPGKGDGVDYVRTEDEGDFRHKSSFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K8C0</accession>
    <name>A0AAR2K8C0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">CAMK2N1</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K8C0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063407.1">
        <property type="protein sequence ID" value="ENSPNAP00000060510.1"/>
        <property type="gene ID" value="ENSPNAG00000036128.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278515"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="77" mass="8553" checksum="3818FA683796E667" modified="2024-11-27" version="1">MSDILEKLPTLQDGFGHFARECNRPGGNFRGNFRGNLRGGFRGQSRPSAGPVNPYRGPELGMMHETICSVKEDFDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KE83</accession>
    <name>A0AAR2KE83_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Citron rho-interacting serine/threonine kinase b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000066593.1">
        <property type="protein sequence ID" value="ENSPNAP00000062585.1"/>
        <property type="gene ID" value="ENSPNAG00000007674.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234517"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20814">
        <property type="entry name" value="CRIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000081">
        <property type="entry name" value="Citron rho-interacting serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000018">
        <property type="entry name" value="Citron rho-interacting serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF71">
        <property type="entry name" value="CITRON RHO-INTERACTING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="1104"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="12">
        <location>
            <begin position="1251"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="703"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="971"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1567"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="59"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="228"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="913"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="703"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="993"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1604"/>
            <end position="1666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1720" mass="197291" checksum="06FC84545D40A8B0" modified="2024-11-27" version="1">MVVRENPSRSAVSEILRPARPATSAIWHCFALKLSSLVPDFFNMHCNPMQRLDSFLFAFVKMEQEISKVQRKMSDLESVLQQKDVELKASETQRTILEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSNKLQEIKEQEYHAQLEEMRVSIRQLEENLSAARRRSDLYESELKESRQTSEELKRKAADYQQKIQKVRGFRGQKHGKHGAQVIDTLTNEEQQAKIEELQDKLAKAVKASTEATELLQNIRLDKERLERDLERLQTREDSTDSLRRRLRETEDGRKTLENQVKRLEIVERRETKLKEDIQTKSQQIQQMADKIMELEENLRDTQATAQRMETHLEQKEKLYEDKIKILENQIKADVADKELLESKQNTYEEESREKGKLISDQAATINAMESKMTSLEQRIAELSEANKLAANSSIYTQKNMKAQEEMISELRQQKFYLESQAGKLEAQNAKLEEHLEKMSQQEQANKSRVLELEARLREIGLEHEEQKLEIKRQVSELTLSLQERESQISSLQAARHALESQLQQAKTELEETTAEAEEEITVLRAHRDEIQRKFDALRDSCAVITDLEEQLTQLTQENAELNRQNFYLSKQLDEATDENEERLQLSQEVDRLRREVADREMHLNNQKQNIETLKTTCTMLEEQVLELETLNDELLEKERQWESWRSALEEEKNQAERRAREIQRQLDNEKQNRLRADQRSSESRQAVELAVKEHKAEILVLQQALKDQKLKAEGLSDTLSDLEKKHAMLEMNARSLQQKLESERELKQRLVDEQAKLQQQLDMQKSHIFRLTQGLQDALDQTDLLKTERTDLEYQLENIQAVYSHEKVKMEGTITQQTKLIDFLQAKVDHPSKKKKGIFGGRRREDLLAALAAQGQTQVPSVPVMTPVPLQYSDMKAALDKERARCSELEEALQKTRMELREAMQFNKALEHGGTPATPGLARQQLMMSTKSNKSPEHQPNPPNLTSSNPGRRKENSNPEGEQYARRVKERLHHSTHGAHNTPHRFTQGLNMRAAKCTVCLDIVHFGRQAATCIECHALCHPKCSPYLPATCGVPTEYALHLSEALCREKGSSSGLALKESGHMRLEGWMKQFRNGKRGQGWERKYMVLEGNKILTYETEPREESVKPVDEFDLYVTDGEVIIHSSVGGSELTNTTKSDVPYVLKLESVPHASCWPAQTLYLLSSGFPEKQRWVAVLEAIVASARGAREKAESDAKLLGNSLLKLEGDDRLDINCTLPLTDQIVLVGSEEGLYALNVIKNSLTHIPGLGSVFQIHIIKEQDKLLMIVGDERALCLVEIKKVKQSLSQSHLPTLPEIVPYIFETVKGCHLFAAGRIENNSCICAAMPNKVTILRYNDNLSKFCIRKEIETLEPCSCIHFTSYSIIIGTNKFYEIEMKQYVLEEFLDKNDVSLASAMFTTSSYSFPIAIMQVSSTPQKEEYLLCFHEFGVFVDAYGRRSRSDELKWSRLPLTFAFREPYLFVTFFNSLDVIELQGHAALGPPALAHLDIPNPRYLGPAISSGAIYLASSYQNKLRVICCKGHLVRESGELQRTGSSRSPNKRGPPTYNEHISKRLASGPGAQEGGLHREPSTPHRYREGRTEFRRDKSPARPLDREKSPGRAVLDSRRERSPGRAFDERERDRERSRLHTSSVRTQLTPVNKVRMKHCHVNLRAIVHSQHGWTCHFTGPSFNTAISSCHCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KQ15</accession>
    <name>A0AAR2KQ15_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 18">Vesicle-fusing ATPase</fullName>
            <ecNumber evidence="3 18">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="18">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity. Interacts with CDK16.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="3 18"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KQ15"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057043.1">
        <property type="protein sequence ID" value="ENSPNAP00000066370.1"/>
        <property type="gene ID" value="ENSPNAG00000009726.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064085"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19504">
        <property type="entry name" value="RecA-like_NSF-SEC18_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000026">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000031">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000006">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000003">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000166">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000187">
        <property type="entry name" value="Vesicular-fusion ATPase SEC18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054419">
        <property type="entry name" value="NSF_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21964">
        <property type="entry name" value="NSF_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 18" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="21">
        <location>
            <begin position="85"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="20">
        <location>
            <begin position="191"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="332"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="615"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="827" mass="91580" checksum="C75A3E60899CE1A7" modified="2024-11-27" version="1">MSVHCLFQIWCVYHSGTVNRFSLSDSGRITLVDACSIFNAVKSCMLTKTKPKLPSSPAWFSFLSHLLFPSLLLALALKVVIFFKTMQAARCPTDELSLTNCAVVSEKDLQSGQHVTVKTTPTHKFVFTVKSHHTVAPGTIAFSLPQRKWAGLSIGQEVEVANYNFDKSKQCIGAMTIEIDFLQKKSTDSSPYDSDKMASEFIQQFNNQAFSVGQQLVFSFCDKLFGLVVKDMEAMDPSILKGEPASGKKQKIEIGLLVGNSQVIFEKAESSSLNLVGKAKTKEARQTIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRLQILSIHTAKMREFKLLSTDVDLKELAGETKNYSGAELEGLVRAAQSTAMNRHIKATATVEVDLEKAEKLQVTRADFMGSLNNDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLEDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGNSEVSKCQAIKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAFSTTIHIPNISSGEHLVEALELLGSFTDAERATIAQQVKGKRVWIGIKKLLMLIEMSLQMDQEYRVSKFLSLLRDEGAMDENNHIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KRD7</accession>
    <name>A0AAR2KRD7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sperm microtubule inner protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017560323.1">
        <property type="nucleotide sequence ID" value="XM_017704834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KRD7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046597.1">
        <property type="protein sequence ID" value="ENSPNAP00000066840.1"/>
        <property type="gene ID" value="ENSPNAG00000032359.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108431585"/>
    <dbReference type="GeneTree" id="ENSGT00390000003224"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054323">
        <property type="entry name" value="SPMIP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35826:SF5">
        <property type="entry name" value="GENE 45521-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35826">
        <property type="entry name" value="PROTEIN ATP6V1FNB-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22589">
        <property type="entry name" value="SPMIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sperm microtubule inner protein 1 C-terminal" evidence="1">
        <location>
            <begin position="66"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="179" mass="20645" checksum="24E7BF243D119A7B" modified="2024-11-27" version="1">MRNLLTTQNQNCYRELIMKEAYTRLTWKMKYSKDYPTSFTTRRAKTIGLFNPPKPSTEITLPPIVQPQGKKKEAPVLVGRSLSEAPLMRPVSPKTKDTLYHGFSKEGKGRSVYLKRRAQKMPEEKFDYPILSSWEYGWRLGDYEHNYRSPANGRSGVVRSTFYARNGIFNIPSATDKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KU16</accession>
    <name>A0AAR2KU16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein Spindly</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coiled-coil domain-containing protein 99</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Spindle apparatus coiled-coil domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">SPDL1</name>
        <name evidence="8" type="synonym">CCDC99</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Chromosome</location>
            <location evidence="8">Centromere</location>
            <location evidence="8">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Spindly family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KU16"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057543.1">
        <property type="protein sequence ID" value="ENSPNAP00000067775.1"/>
        <property type="gene ID" value="ENSPNAG00000018057.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047951"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007080">
        <property type="term" value="P:mitotic metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03041">
        <property type="entry name" value="SPDLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028593">
        <property type="entry name" value="SPDLY_chordates"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF9">
        <property type="entry name" value="PROTEIN SPINDLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 8" id="KW-0137">Centromere</keyword>
    <keyword evidence="1 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="3 8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="477"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="8"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="214"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="614" mass="70939" checksum="DB0D3B416F2A708B" modified="2024-11-27" version="1">MSATDGEIQTLRQKVEETEVALQKAAQYGLQLLDDKMDLQNKLEEQRIEMTAVIEVLEQEKYSLQREVELKGRMLDSLHSEFDYVKNQQKHLLEQQQTMLERNHAVELCDFKSKVEKIKADLDEARLLEKQMRHKLELQSQTLSCKTEELRKLMECSHDTRSSEIAELQMKKMDLESSVTTLKRELQESDYKGQQLQLTVTIIQRQLEAMTSLKEEKDKEVESLYNALEKSREANQDLQIQLEQALQQMQDPNSKGNSLFSEVEDRRVEMERQLISVKVQYQSLQKQHAFTKQQMLRMKVFRKSNFVSNKESGFFLLLPPPPPPPPLYVVQVQISNLMQLQGVRADPAQLERLQFMLSEKNSEIETLMRKVQQLEKMTLEDQSSTGTSKPNEFVDETYYTDLLKMQLSSSKKVAETLKDELSMARLKALSESHRVLELERKLFGAENALKQGQSDNIKLQVKLEELQMKYEPEAVNRARAQKRKREKFPANPPEERSDHCTMETPATDNNPVKIPDMTAETPPCSTKNPPAKAQAPDCSSVLLDGSKCVRICEDTQISLPEPPKSSVADCSSMAEQQILKCDEEQENWRAVERKYHMPVHVDPENTMERQCAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KYG3</accession>
    <name>A0AAR2KYG3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein eL24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">60S ribosomal protein L24</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KYG3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068348.1">
        <property type="protein sequence ID" value="ENSPNAP00000069310.1"/>
        <property type="gene ID" value="ENSPNAG00000011883.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183105"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000004">
        <property type="entry name" value="60S ribosomal protein l24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="TRASH" evidence="10">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="125"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="169" mass="19226" checksum="1B5D222C2D859FF2" modified="2024-11-27" version="1">VSFFDIYSLFVTCRVELCSFSGYKIYPGHGRRYARIDGKVFQFLNAKCESAFLTKRNPRQINWTVLYRRKHKKGLSEEVTKKRTRRAVKFQRAITGASLADILAKRNQKPEVRKAQREQAIRAAKEAKKAKQAAKKVPAPTTKAHAKAAPKQKIAKPMKVNAPRVGGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KYW7</accession>
    <name>A0AAR2KYW7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RUVBL2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Proposed core component of the chromatin remodeling Ino80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Dynein axonemal particle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KYW7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052817.1">
        <property type="protein sequence ID" value="ENSPNAP00000067572.1"/>
        <property type="gene ID" value="ENSPNAG00000002395.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153556"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003351">
        <property type="term" value="P:epithelial cilium movement involved in extracellular fluid movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060420">
        <property type="term" value="P:regulation of heart growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002221">
        <property type="entry name" value="RuvB-like 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000002">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="65"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="471" mass="52317" checksum="396C2EEF6B5E25CE" modified="2024-11-27" version="1">MATTKVPEVRDITRIERIGAHSHIRGLGLDDALEPRQVSQGMVGQLASRRAAGLILEMIKDGHIAGRAVLIAGQPGTGKTAIAMGIAQSLGPDTPFTALAGSEIFSLEMSKTEALSQAFRKAIGVRIKEETEIIEGEVVEIQIDRPATGTGAKVGKLTLKTTEMETIYDLGSKMIESLSKEKVQAGDVITIDKATGKISKLGRSFTRARDYDAMGSQTQFVQCPEGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEVREQINAKVSEWREEGKAEIIPGVLFIDEVHMLDIECFSFLNRALESDLSPVLIMATNRGITRIRGTNYQSPHGIPIDMLDRLLIIATSPYTEKETRQILKIRCEEEDVELSEEAHTVLTRIGQETSLRYAIQLISTAGLVCRKRRGTEVQVEDIKRVYSLFLDESRSSQYMKEYQDSFLFNETLKFVLKTVNSPSILWITPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L2M1</accession>
    <name>A0AAR2L2M1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">B3GNTL1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037399668.1">
        <property type="nucleotide sequence ID" value="XM_037543771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L2M1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046005.1">
        <property type="protein sequence ID" value="ENSPNAP00000070863.1"/>
        <property type="gene ID" value="ENSPNAG00000036326.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108414673"/>
    <dbReference type="GeneTree" id="ENSGT00390000006933"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06913">
        <property type="entry name" value="beta3GnTL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="45"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="385" mass="43730" checksum="9D6CE94ADEE19AC4" modified="2024-11-27" version="1">MISSLKHVGDSEGPCAVVKRVCRRGCWEDGTVEVESTEGASVQVSVIVPVYNAAEWLEECLQAVLDQDFKGSMELSVYDDSSTDNSREIVEGWREKLEKRGISVVISGHSSDKPRGVGFAKNQAVSQSSGECLCFQDADDVMMPQRVRLQYETAALHRTAIVGCQVCRVPEGSTERYTRWINSLSPEQLYTQVYTSHGPTVVMPTWFCSRDWFLKVGPFDEGGKGVPEDLLFFYQSLRLGGSVIRVDECLMVYRYHQNAATHSVLEETIWTLRVDFLQERVLNQWDSFTIWNAGKQGRKLYRSLNPTNQKKVKAFCDVDENKIKKGFYTYEESKERPKPKIPVLHFRDASPPFIVCVKLDMTGGVLEDILLSLKLTEGKDYYHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L6H3</accession>
    <name>A0AAR2L6H3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-nucleotidase domain containing 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NT5DC2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L6H3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060076.1">
        <property type="protein sequence ID" value="ENSPNAP00000072145.1"/>
        <property type="gene ID" value="ENSPNAG00000007753.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164911"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07522">
        <property type="entry name" value="HAD_cN-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000026">
        <property type="entry name" value="NT5DC3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02244">
        <property type="entry name" value="HAD-IG-Ncltidse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF19">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="68432" checksum="117F548C927BBA9D" modified="2024-11-27" version="1">MSLQRVGGLGRALWQSGSRTPPLARKDRNFSSKRLAPCAACSAEQSCRDGLCKGASPPAVPPASPAGSRHQIGGKARSEESLNVSGRSFSSTALKVEQTSYLWARYNEMKRLVHDLIPPGVCNLLNSSTIYANNEVSLSEVDIYGFDYDYTLALYSNALDAMIYTNARDFLIKHFKYPQAISKYDYFPNFAARGLHYDIQKGLLMKIDAFHYIQLGTVYRGLNPVPDEEVLRLYDGTHHVPLNQVSGFYGKGPNVKQFMDVFSLPEMTLLAVANDYFISNDIEYDPVHLYKDVSEAIGMVHIKGYMYKWIMEDLEKYILRGEETYAVLHRLVSHGKKLFLITNSPFSFVDKGMRYMVGKDWRDFFNVIIVQADKPHFFNDCAKPFRRLDSKGDLQWDKISNLDKGQVYKQGNLFDFLRLTGWRGSKVLYFGDHLYSDLADLMLRHGWRTGAIVPELELETKVVNTEQYAQSLTWLQALTGLLERMQMYRDPDSRAVLQEWLKEREELRARIKNLFNPQFGSIFRTCHNPTYFSRRLCRFSDLYMASISCLLNYDLSYTFYPRRTPLQHEAPLWMDQLCTGCMKTPFLEEMAHIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L7W4</accession>
    <name>A0AAR2L7W4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564241.1">
        <property type="nucleotide sequence ID" value="XM_017708752.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L7W4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057015.1">
        <property type="protein sequence ID" value="ENSPNAP00000070742.1"/>
        <property type="gene ID" value="ENSPNAG00000035740.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433892"/>
    <dbReference type="CTD" id="100148748"/>
    <dbReference type="GeneTree" id="ENSGT00400000022086"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000020">
        <property type="entry name" value="CDK5 and ABL1 enzyme substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012388">
        <property type="entry name" value="CABLES1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896:SF4">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025798">
        <property type="entry name" value="Cables"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="5">
        <location>
            <begin position="401"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="511" mass="55665" checksum="D4F5024881CBD540" modified="2024-11-27" version="1">MAAAACGPYAGAGCNTTKPAHKEVRKSKDSRRRQAALLFLNNISLDGRPVCALSNGAASLRETGHQSAEAGVPAAAPPLSALSTAASYGAFSKLSAPVSGGTNAAPLPRTSTYPSALLPTTLAGFSQGSSDVFSEGSTVKVDPLSSQGFPFSPTSGPQNPSLVSCKSSVVCSSYNSAPLDLRQRTRNQSGSPGPRAKKVHFIKNMRQYDTRGSRIVLICAKRSLCTAFSVLPYGESFHISDSKLEAQRQRHPSGGVADMAPVLEGVELGAFGKTVSYAQFLLPTNALVRQKSNAPLDTNVQTPMPRNWINGQKNFTPSRLSSAIGQETGVDEISDYDPNLLSDPQWPCGKHKRVLIFASYVTTVIEYVKPSDLKKDMNETFKEKFPHIKLTLSKIRSLKREMRAVSEECGLQPVTIAMAYVYFEKLVLQGRLNKHNRKLVSAVCVLLAAKISSDLKKQEVKLLIDKLEERFRISRRELIPLEFPVLVALEMALYLPDSKVMPHYRRLVQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LCV9</accession>
    <name>A0AAR2LCV9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Small integral membrane protein 24</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PDZK1-interacting protein 1/SMIM24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LCV9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050575.1">
        <property type="protein sequence ID" value="ENSPNAP00000074438.1"/>
        <property type="gene ID" value="ENSPNAG00000030149.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000201079"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031627">
        <property type="entry name" value="PDZK1IP1/SMIM24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15296">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROTEIN MAP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15296:SF2">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15807">
        <property type="entry name" value="MAP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043927502" description="Small integral membrane protein 24" evidence="7">
        <location>
            <begin position="21"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10701" checksum="CAEFDCEE8BFC3289" modified="2024-11-27" version="1">MFHLRNLLACLLLSVSICQARTGGLRTASGKVTLQPWLVGLSAVVGFLFIVFFILILKRIFFKKDKDERETDFEGYENKALDLEIAEETKTSQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LFG0</accession>
    <name>A0AAR2LFG0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DDX5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LFG0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046394.1">
        <property type="protein sequence ID" value="ENSPNAP00000075338.1"/>
        <property type="gene ID" value="ENSPNAG00000000825.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154705"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="146"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="349"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="493"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="570"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="534"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="570"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="643" mass="72220" checksum="1DB11B1036DB1D8C" modified="2024-11-27" version="1">AETEFIRPVTCLRLVSRLVHFLLYRGSNIGGRVLYGNGPPRFGGSRGGPPQGKKFGNPGDRLRKKHWNLDELPKFEKNFYQEHPDVVRRSSQEVEQYRRSKEITVKGRDCPKPVMRFHEASFPSYVMDVINKQNWTDPTPIQAQGWPLALSGKDMVGIAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYGRASRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLEAGKTNLRRCTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYVQINVGALQLSANHNILQIVDVCSDGEKEDKLVRLLEEIMSEKENKTIIFVETKRRCDDLTRRMRRDGWPAMGIHGDKSQQERDWVLNEFKFGKAPILIATDVASRGLDVEDVKFVINYDYPNNSEDYIHRIGRTARSQKTGTAYTFFTPNNMKQAHDLVSVLREANQAINPKLIQMAEDRGGRSRGGRGGGYRDDRRDHYSGGRRGFNSYGQDSRSNDRGYDSGPKKVFGNKAQNGGAYGSSNYNGNSNNGFNGAYNNGQSSFGGNQSGGFGNPGFQNQQFGASQGGIQNGMNHNQFPFNPQQQPPQPMPQQMVPFSMPPPAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LKS1</accession>
    <name>A0AAR2LKS1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LKS1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080206.1">
        <property type="protein sequence ID" value="ENSPNAP00000074926.1"/>
        <property type="gene ID" value="ENSPNAG00000035101.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064158"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="31"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="428"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="516"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="529" mass="58777" checksum="FF0C8993A443663D" modified="2024-11-27" version="1">MPFLCVSQTKNQKAARVTSASVAEEEYGTVLHSFVFHRGLVGKNVDQLVTDMRRVMEPYTARALKVLTEPVKNVLKDFVTAAGPLGVTHFLIFTKTETNVNLRLARLPKGPTLFFHVTKYSLIKDVVSSLKRHRMHEQQFSHHPLLVLNNFALDGMHIKLMATMFQNMFPSINVHTVNINNIKRCVLLTYDPESKEIDFRHYLKVVPVGMSRGVKKLMQEKLPNMNKLEDISDLLLQGVNLSESEAEQDGDHNITELPQVYSGRGNMRSQQSAVRLTEIGPRMTLQLVKITEGMGDGNVLYHSILKSNCMIETQVSTNHRLRFYIVLKLSDYRKKSLEGMKKKRQQEGAGSDDSEVEDPGMQEDQAAAEESEDEAEYYRQAVGQEPDEDMFPTAKRKHGPGKSPRPFKKRKTSSSKASDEKQSPRPAGKPAFGGKASAGKKFGGKKFGVGGKGFGARKTAGTGSKFGKSKWGKKFGENKRDGFGGNKNGPKKGPSSKLKGQRSKTGSGFKAGPRGGKAKKDFKQKKARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LM63</accession>
    <name>A0AAR2LM63_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Protein SYS1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein trafficking. May serve as a receptor for ARFRP1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with ARFRP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SYS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563882.1">
        <property type="nucleotide sequence ID" value="XM_017708393.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390669.1">
        <property type="nucleotide sequence ID" value="XM_037534772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LM63"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063922.1">
        <property type="protein sequence ID" value="ENSPNAP00000077645.1"/>
        <property type="gene ID" value="ENSPNAG00000033264.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433675"/>
    <dbReference type="CTD" id="90196"/>
    <dbReference type="GeneTree" id="ENSGT00940000154347"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016973">
        <property type="entry name" value="Integral_membrane_SYS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019185">
        <property type="entry name" value="Integral_membrane_SYS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952:SF0">
        <property type="entry name" value="PROTEIN SYS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12952">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09801">
        <property type="entry name" value="SYS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031402">
        <property type="entry name" value="SYS1_homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR031402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="156" mass="17862" checksum="CA6DBA2CBFFB430A" modified="2024-11-27" version="1">MASHFRSYIWDPVLIISQIILMQCIYYSFLGLWMAGVDGLVQTNRSLDQIFSYEVLGFSTTQGRLSMMAFILNSLTCALGLWFFIRRGKQCLDFTVTVHFFHMIGCWIYNSHLPVALSWWLVNMACIALMAVIGEYLCMRTELRAIPVNSGPKSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LQ81</accession>
    <name>A0AAR2LQ81_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LQ81"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076627.1">
        <property type="protein sequence ID" value="ENSPNAP00000076466.1"/>
        <property type="gene ID" value="ENSPNAG00000030195.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177809"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="193" mass="21982" checksum="D090D1D56F0BDAD4" modified="2024-11-27" version="1">MLIAPRLHYTNGLQKEGKKRDCWQRKGGRERGRKGGDRRERKGEYFPSLSISQRSHNATLHANKQRKATAMLGNASLFQVLPWKTVSVSRLSPCSLQPHLSNAKEHSFNCMWILQNVRNGEETTGETVAGHRLMSSVLNHRTAEWFYAYFICISLNPARLLSGNLPIRFNSTLIKKSGCAQPFKRGLIEHRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LSI9</accession>
    <name>A0AAR2LSI9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome 20S subunit beta 11b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PSMB11</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LSI9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067156.1">
        <property type="protein sequence ID" value="ENSPNAP00000079563.1"/>
        <property type="gene ID" value="ENSPNAG00000026338.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162200"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF15">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="193"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="39931" checksum="DF19F900F4E4CEFE" modified="2024-11-27" version="1">MALQDVCGFLNSREDTTFMLGKRSGSPIEFGQIRPSFDSDWTNPKADDHLPYSLPSISVQSAIPLYISPSVPLPFPLGHGTTTLGFVFQGGVIAAADSRSSCAGLVACPESQKIIPVHSHLVSTTSGTSADCALWKRILARELRLYQLRNRRRLSTGGAAKLLSHMLHPFKGTELCVAATLCGWDGDEFLGGDERSMDAGTQSKGTDGKKTGSVSEASFQPVAEPDAKTQPTASNKTSSTLITTKQTGPRLYYVCSDGLRLQGELFSVGSGSPYAYSILDGNIHWGLSVDEAVSVAREAVYRATHRDAYSGNNVDLYHVTAKGWRRRKREDLKEEYYREKERERKRVEERRREIENKNFDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZP3</accession>
    <name>A0AAR2LZP3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calpain-1 catalytic subunit</fullName>
            <ecNumber evidence="6">3.4.22.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Calpain-1 large subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Broad endopeptidase specificity.</text>
            <dbReference type="EC" id="3.4.22.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.52" evidence="6"/>
    <dbReference type="RefSeq" id="XP_037390354.1">
        <property type="nucleotide sequence ID" value="XM_037534457.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390355.1">
        <property type="nucleotide sequence ID" value="XM_037534458.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390356.1">
        <property type="nucleotide sequence ID" value="XM_037534459.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390357.1">
        <property type="nucleotide sequence ID" value="XM_037534460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LZP3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045230.1">
        <property type="protein sequence ID" value="ENSPNAP00000079816.1"/>
        <property type="gene ID" value="ENSPNAG00000006646.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443488"/>
    <dbReference type="GeneTree" id="ENSGT00940000158672"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000124">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF322">
        <property type="entry name" value="CALPAIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="14 19" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="20">
        <location>
            <begin position="48"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="606"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="active site" evidence="18 19">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" evidence="18 19">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="active site" evidence="18 19">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="704" mass="80359" checksum="0C122B5637C7A1EC" modified="2024-11-27" version="1">MEIFGGMSARIHRERLHAEGVGSNERALQIFSQDYEALKQESLESGRLFEDSCFPAQPPSLGFKELAPHSSKTRGVTWKRPTELTESPQFIVGGATRTDICQGALGDCWLLAAIASLTLNEKLLHRVVPHGQSFTDDYAGIFHFQFWQFGEWVDVVIDDRLPVKDGELMFVHSAEGSEFWSALLEKAYAKLNGSYEALSGGSTTEGFEDFTGGVAEMYELRKAPRDLHRIIAKALERGSLLGCSIDITSAFDMEAITFKKLVKGHAYSVTGLTEVNFRGNRERLIRVRNPWGQVEWTGAWSDNSSEWNAIDPAEKEEMNSKMEDGEFWMSFQEFKRQFSRLEICNLTPDALNDDSLSYWNTIKFEGGWRRGSTAGGCRNHANTFWINPQYKITLLEEDDDPEDDEKACSFLVALMQKDRRRYRKQGQDMHTIGFAIYEVPEEFQGCQSVHLKKDFFLRHSSCARSETFINLREVSSRLRLPPGEYLIVPSTFEPGKEADFVLRVFTEKQSETQEMDDDVSFILDEEGEITEQDIDASFRAMFAQLSGEDMQISVHELRTILNRVVGKHRDLKTDGFSLEACRCMVALMDKDGSARLGLVEFQILWNKIRKWLGIFRQFDLDKSGTMSSYEMRLALESAGFKLNNKLNQVLVARYADQEMIDFDNFICCLVKLEAMFRTFQQLDRDGTGLAELNLSEWLYLTMCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M2G0</accession>
    <name>A0AAR2M2G0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C1orf210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M2G0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064418.1">
        <property type="protein sequence ID" value="ENSPNAP00000080806.1"/>
        <property type="gene ID" value="ENSPNAG00000036977.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234931"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31450">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 19 LRRC19 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31450:SF3">
        <property type="entry name" value="TYPE III ENDOSOME MEMBRANE PROTEIN TEMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15176">
        <property type="entry name" value="LRR19-TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="106" mass="12032" checksum="14B9F0933DE13F77" modified="2024-11-27" version="1">PIIIRYTILYGDIKTSMRLPYCNLVNGSWKLLVAVLVTAIIISILIAVGAKCKIVHKYLASYRHSRLSSAEGGEMEEDDDGFIEDNYIQASERERCTFEFSLHFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M3N9</accession>
    <name>A0AAR2M3N9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CD37</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017551442.2">
        <property type="nucleotide sequence ID" value="XM_017695953.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M3N9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060828.1">
        <property type="protein sequence ID" value="ENSPNAP00000081739.1"/>
        <property type="gene ID" value="ENSPNAG00000008159.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426468"/>
    <dbReference type="GeneTree" id="ENSGT00940000161485"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF44">
        <property type="entry name" value="CD82 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="34200" checksum="2314901F20BDAB99" modified="2024-11-27" version="1">MASEFCLSLTKYFLFVFNLVFFFLGSALLSLGLWIMFSETTVLIPAPRFISLSLFSYLLIISGVVTISLGFFGSLGALKEVKCMLAIYFILLTVLLVAQTMGGVLLITQKSLFEKSLESHVQTLIKDFGKNDSDLRDFERTLVVLQREAKCCGWSGPDDWKPKKHCSCYSPNNFTANVTDHSDHSDTYTCNVTTWHLHEKGCKEYVEEWLSGHILIILMVVFVLAVVEVRYNCAVFSFFHHTSTAKAQLFFVQNHCKVLLLMLLQLLKAAQNKAYSIYYKAEMQRVLLCRTVNFPCPLCVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M4N0</accession>
    <name>A0AAR2M4N0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">P2X purinoceptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">P2RX7</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for ATP that acts as a ligand-gated ion channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the P2X receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574587.2">
        <property type="nucleotide sequence ID" value="XM_017719098.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M4N0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086595.1">
        <property type="protein sequence ID" value="ENSPNAP00000082102.1"/>
        <property type="gene ID" value="ENSPNAG00000010872.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440304"/>
    <dbReference type="GeneTree" id="ENSGT01020000230351"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004931">
        <property type="term" value="F:extracellularly ATP-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.490.10:FF:000001">
        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.490.10">
        <property type="entry name" value="atp-gated p2x4 ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003050">
        <property type="entry name" value="P2X7_purinoceptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027309">
        <property type="entry name" value="P2X_extracellular_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001429">
        <property type="entry name" value="P2X_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00863">
        <property type="entry name" value="P2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF13">
        <property type="entry name" value="P2X PURINOCEPTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01314">
        <property type="entry name" value="P2X7RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01307">
        <property type="entry name" value="P2XRECEPTOR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="15">
        <location>
            <begin position="379"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="583" mass="66239" checksum="9A581FF4145A0658" modified="2024-11-27" version="1">MACGLLNLCVYETNKRFRIKSVELGTLKWTLNGAVLLFICFMMLWNKEYQEHDLVTSSVTTKVKGVALASIPEMGLVVWDVVDHSGLAQGKNSFFVATNVIVTKNQKQGKCPEVAPWGLCKTDKDCQKGFSDQQSHGVQTGPCVKFDVLKKTCEVSAWCPVENRKSPPRPALLASAENFTVLVKNNIRFPAFNYIRRNILPEMTDKDLKGCLYNRYTNPFCPVFRLGDIVHEAKERFSDMAVEGGVIGIQIDWNCDLNRFFHSCLPKYSFRRLDEKESNRTLYPGLNFRFARYSTINGVEHRTLFKVYGIRFDVMVFGKAGKFSIIQLIIYIGSTLSYYALTTVFIDWLIGTNCYSRKAKHNYSEKQCESVQDQKYLLCVSFVDEEQLRVVKMSRNKRLQETKPLSQHQWKGNLGDMRALLGVLQSEQNINALIQNGQCNEFKAATALSTEHSGLVTDMPLLEISSSRCPDWCQCGCCHSSASFLEQLCCRKSKGRCITTSSLFPALVLTRSILEIALLYVAPLAEQHKDDQLRQQAYAQYIKWRFGDFTPMDAVPVIPSCCVWRIREQYPSPDGKYSGIIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BN70</accession>
    <name>A0A3B4BN70_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Four-jointed box kinase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FJX1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017578269.1">
        <property type="nucleotide sequence ID" value="XM_017722780.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BN70"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001212"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012554.2">
        <property type="protein sequence ID" value="ENSPNAP00000001212.1"/>
        <property type="gene ID" value="ENSPNAG00000008022.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442640"/>
    <dbReference type="CTD" id="24147"/>
    <dbReference type="GeneTree" id="ENSGT00390000016768"/>
    <dbReference type="OMA" id="VEDDIFW"/>
    <dbReference type="OrthoDB" id="10055077at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024868">
        <property type="entry name" value="FJX1/FJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13147">
        <property type="entry name" value="FOUR-JOINTED BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13147:SF5">
        <property type="entry name" value="FOUR-JOINTED BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02072">
        <property type="entry name" value="4JOINTEDBOX1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017458120" description="Four-jointed box kinase 1" evidence="1">
        <location>
            <begin position="20"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="41237" checksum="BFB672C48ECD69C2" modified="2018-12-05" version="1">MRTNSLSFFLALLMRASLCTPSPRASRTESASCSSSSFSTLDSSRVSTVEEAVRVQEQGGTTLRARFPAGFGAAVARRWSEWRERTGKARVVRLEAGCGRTTNRLATLEDGSRACVRYGVDQDQVLGETLCYYLSTMLGIPNVPPLVLTKPDPFSQQWSAVRDSIEALNWSSNAIVSLTQWVSNATRVSIPVQLLRKLGTGVHPAPLTLENMTSSDLRELKQWTDLIVFDYLTANFDRVASHLFNLQWDRRAMDRLTNNLLRTTKGQLVFIDNEAGLVHGYRVLDRWEHLHQTLLNSTCMFRESTVQRLTELTLCRSAAQRLRQVYQTREPLALELGFLSEAQMLTLQNRVDTLYRHFQQCGRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BP99</accession>
    <name>A0A3B4BP99_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017550455.1">
        <property type="nucleotide sequence ID" value="XM_017694966.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017550456.1">
        <property type="nucleotide sequence ID" value="XM_017694967.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017550457.1">
        <property type="nucleotide sequence ID" value="XM_017694968.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037402601.1">
        <property type="nucleotide sequence ID" value="XM_037546704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BP99"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001587"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011908.2">
        <property type="protein sequence ID" value="ENSPNAP00000001587.1"/>
        <property type="gene ID" value="ENSPNAG00000000820.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425903"/>
    <dbReference type="CTD" id="556208"/>
    <dbReference type="GeneTree" id="ENSGT00390000001360"/>
    <dbReference type="OMA" id="EHATATC"/>
    <dbReference type="OrthoDB" id="10069557at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22395">
        <property type="entry name" value="KH-I_AKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20407">
        <property type="entry name" value="Tudor_AKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047368">
        <property type="entry name" value="KH-I_AKAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047367">
        <property type="entry name" value="Tudor_AKAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF65">
        <property type="entry name" value="A-KINASE ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="866" mass="93708" checksum="D91C1C3E8C99E037" modified="2018-12-05" version="1">MPLRFRSIVPYIFPGVVALIGWWWYTSRKKEWITGHNSEEGQAMGLLASPGESSNGIVDKDCQAISVRHGRTSQERPKAKEQRPAEQEVVAQVHAPATKASSGEPLSSSAIVEASGKLDSSHSKATTDSLQSSPEAFRQKPPVPTSTSSNVVEKTSHKRPVAAALSDKSRQLPTVGELDSVRQLQDQTANKQELDTKEISPAVPWSAELPKAERPEPEGGVADDILAVAFAENVQADLVKDSLSSTKDDLPPKTLTVLDQCAQAVFQHSFLTESPVVSASFPLSASTPVSDNSIEAKHHENGDVSESAKTSNSMEELHRLATGLITQVITAATQEVMAVSSCEASVATSSITPTFNGKDIMREEEHRESQVCPSSQSQANHVEQRTAQTAEDVINGCLVPSVWEKSEDERQCTLSSQRQLESIPVLPKIQAEEAVTGTEDSGCSTCQSEDGISSEDLLLSTGLSSTVPERKEDLIQISGISRSSEEKEDGLQESSDLTGLEEPTLTAEHVAAVCEVARLNGAGLRNGTSEAEADQSGGSDVNSMDSVDSCSTLGTGDGQQSGSQSSQSQSSELIVWEIEVPKHLVGRLIGKQGRYVSFLKQSSGAKIYISTLPYTQEFQICHIEGTQQQVDKALALIGKKFKDLDLTNLYAPPPLPLSLPSLPMTSWLLLPNGVTVEVIVVNIVSAGHVFVQQHTHPTYHALRSLDQQMFLCYSQPGTPALPSPAEVGVICAAPAVDGAWWRAQVISFYKDSNEVEIRYVDYGGYDRVKIDTLRQIRSDFVTLPFQGTEVLLDNIAPLPGEDRFSTEATSALEEMTRGVPLLAQVTNYDNNTGLPLVQMWNMVGDDLVQLNRTLAERGFATWVDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPR1</accession>
    <name>A0A3B4BPR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S-adenosyl-L-homocysteine hydrolase NAD binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AHCYL1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577498.1">
        <property type="nucleotide sequence ID" value="XM_017722009.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPR1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000444"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013837.2">
        <property type="protein sequence ID" value="ENSPNAP00000000444.1"/>
        <property type="gene ID" value="ENSPNAG00000007517.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442121"/>
    <dbReference type="CTD" id="10768"/>
    <dbReference type="GeneTree" id="ENSGT00950000182981"/>
    <dbReference type="OrthoDB" id="10007170at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000002">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000007">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000035">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000009">
        <property type="entry name" value="Adenosylhomocysteinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF3">
        <property type="entry name" value="S-ADENOSYLHOMOCYSTEINE HYDROLASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="7">
        <location>
            <begin position="313"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="554" mass="61388" checksum="FD6201153D740461" modified="2018-12-05" version="1">MTESGVDAKLEVKQASKEFKESENVAEKYSAMTVSKNSDVNMNVGELSAAFTAVPAHKSVKKQIQFANQEDKQEFSKFPTKTGRRSLSRSISQSSTDSYSSAASYTDSSDDETSPREKTQVNSKGSSDFCVKNIKQAEFGRREIEIAEQDMSALISLRKRAQGEKPLAGAKVVGCTHITAQTAVLIETLVALGAQCRWTACNIYSTQNEVAAALAEIGVAVFAWKGESEDDFWWCIDRCVNTEGWQANMILDDGGDLTHWVYKKYPSVFKKVQGIVEESVTGVHRLYQLSKAGKLCVPAMNVNDSVTKQKFDNLYCCRESILDGLKRTTDVMFGGKQVVVCGYGEVGKGCCSALKALGAIVCVTEIDPICALQACMDGFKVVKLNEVVRHMDMVITCTGNKNVVTREQLDRMKNGCIVCNMGHSNTEIDVASLRTPELTWERVRSQVDHIIWPDGKRVILLAEGRLLNLSCSTVPSFVLSITATTQALALIELYNAPEGRYRQDVYLLPKKMDEYVASLHLPNFDAHLTELSDEQAKYMGLNKNGPFKPNYYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQY8</accession>
    <name>A0A3B4BQY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">KHNYN</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQY8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002117"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010987.2">
        <property type="protein sequence ID" value="ENSPNAP00000002117.2"/>
        <property type="gene ID" value="ENSPNAG00000008647.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183002"/>
    <dbReference type="OMA" id="ENINHYR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002142">
        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060088">
        <property type="term" value="P:auditory receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10833">
        <property type="entry name" value="PDZ1_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10834">
        <property type="entry name" value="PDZ2_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06751">
        <property type="entry name" value="PDZ3_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000090">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000092">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000171">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051844">
        <property type="entry name" value="USH2_Complex_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116">
        <property type="entry name" value="PDZ DOMAIN CONTAINING WHIRLIN AND HARMONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116:SF29">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="35"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="159"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="682"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="477"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="650"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="613"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="653"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002117.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="777" mass="85796" checksum="226C3A42D9F83BE5" modified="2024-11-27" version="2">RPSPSLITDCMTQTQQTEIKIKNAGILINLGERLTVTVDQTQHGRLGFSVRGGAEHGLNIFISKVEPSSAAEQAGLCVGDKLLEVNGISLENISMSSAVKVLTGHRRLRLVLQRVGWVPGVRYCNEKTTWVDLIHRRMVVDEGGAAVPDRRSDAALCRTVHLQTSLCQPYLGLNIRGGTEYGLGIYVSKLDPGGLAEQGGIKMGDQILSANGVSFEDVTHSRAVEVLKSQSHITLIIKEAGRYPAYQEMVAEYTWMDKRLCAGSSKMFHSSSQGSDSHSSASSLSSGTLLSSMTGLSQATLPLCQPFGGQMADMSSFIEGQFSLDHSDEAVHTEQMETSLSRGPTELLQDSAIRYEGGEENRREGEEKKEGRKTSVLMALSRPSPPVHRTLSHMTSISLHIHYGICCKKKRFCCVLQYSREQELKALVLPLLAILDRPEKLLLLREIRMLVIKDDLQQFNSMVAPFEEEAYDVLKRRSRRSSPLRSPKAGHTPKRHLITPVPDQQGGFELQDAEDLDRQNRLLEELKHLHVTQQQGEMSEASRSFSPLLDIPVDSYSHSDSLKPSRKSPMLPNWLLTKNTSPATDNGMVISDEDQSHLENSGTKKWRFPFRNGHSENKANRPENEEQDVFFTVVDTTRPKRPLLSQVFGPLKRSQSSTVPSTGHSGQNHHDGHTDQDYELKTVSISKTKQSLGISISGGVESRVQPMVKIEKIFPGGAASSNDILKGGFELVSVDGVSLQGITHQDAVEIIRQAFSNKLVDPMELVVKVPSTPESTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BV39</accession>
    <name>A0A3B4BV39_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Roc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MFHAS1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017569338.1">
        <property type="nucleotide sequence ID" value="XM_017713849.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BV39"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003512"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008592.2">
        <property type="protein sequence ID" value="ENSPNAP00000003512.1"/>
        <property type="gene ID" value="ENSPNAG00000005011.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437022"/>
    <dbReference type="CTD" id="9258"/>
    <dbReference type="GeneTree" id="ENSGT00940000158928"/>
    <dbReference type="OMA" id="IVCPKNG"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057263">
        <property type="entry name" value="COR-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF48">
        <property type="entry name" value="MULTIFUNCTIONAL ROCO FAMILY SIGNALING REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25497">
        <property type="entry name" value="COR-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Roc" evidence="4">
        <location>
            <begin position="386"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="993" mass="112755" checksum="651949715837FD0E" modified="2018-12-05" version="1">MAENETNLKTARLWRDAALRSRKLRSSLRHLTLSTKNSQTITLPEDIKEIEVLNLGNNSLQELPEGLGSTLTRLRILILRRNKFAAVPSAVFQLSQLVELDISHNCLGHFSEDIDLLKGLKKLCISHNKIQYLPSQIGTLQCLEELDISFNELHDFPRSFAQLRRLRTLDVDHNKLQRFPPEILALSDLEELDCSGNKLEGLPGNIMMLQSIKILWLSSTHLSSLPETFCDLQNLESLMLDNNLLTALPQSFGKMQKLKMLNLSSNSFEDFPQVILKITSLEELYLSRNRLSFLPEEVGQLSNLANLWLDNNRITFLPDSIVELEKLEELVLQGNQIAILPDNFGKLAKVNIWKVKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVKPRLKLVLMGQRNAGKTRLRQCIVSKPLDAKTIPGARGIDVTNWVADANRSLTFIVYDLSGKQNYDLIKPFFLSPGALYILVVNLKSYTCKSFYSSVGYFLHLLSAKVPHAVVCIVGTHTDLCDDSELEEKCLDIHMQISLQEKRDIDCLQMLAVQVDQALEQGYDVRASSPHVLFYGVSDKNLRRKKSQLQYMLNNRLQILSPVFCVSCMEAQKNIQRLKEKLMSVADHREIFPNLHRVLPKSWQMLEELHFKPKDLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSITLKEYVFHNLPRFIAILNVFFQKDLAAMLEKLHSEGEGGDNATASQMQQHVEGFLLHGLLPSNVIRLLLKPLVQTQQDLHLIMELLEKMGVCYCVNKPRSKPLNGATVLYKFPSLVSSEEAHAEPWMNGGSLIPGQFFSVEQLQIEYSFPFLIPPGLFARYSVQINNHVVQRSDGRHYIFAYRGKVPVMVSYRPSRNRLQPEMLAISSHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYSVHVLCSKCLKRGSPNPHSFPGELLSQPRPEGLTEIVCPKNGSERVNVALVYPPSPTLVSPSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZ69</accession>
    <name>A0A3B4BZ69_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Chromaffin granule membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017579054.1">
        <property type="nucleotide sequence ID" value="XM_017723565.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017579055.1">
        <property type="nucleotide sequence ID" value="XM_017723566.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017579056.1">
        <property type="nucleotide sequence ID" value="XM_017723567.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017579057.1">
        <property type="nucleotide sequence ID" value="XM_017723568.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397240.1">
        <property type="nucleotide sequence ID" value="XM_037541343.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397241.1">
        <property type="nucleotide sequence ID" value="XM_037541344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZ69"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003880"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007952.2">
        <property type="protein sequence ID" value="ENSPNAP00000003880.2"/>
        <property type="gene ID" value="ENSPNAG00000010331.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443129"/>
    <dbReference type="CTD" id="567877"/>
    <dbReference type="GeneTree" id="ENSGT00940000157586"/>
    <dbReference type="OMA" id="SPYVKIN"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042584">
        <property type="term" value="C:chromaffin granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031045">
        <property type="term" value="C:dense core granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08402">
        <property type="entry name" value="C2B_Synaptotagmin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21964">
        <property type="entry name" value="Syt2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000007">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000016">
        <property type="entry name" value="Synaptotagmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF379">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="149"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="280"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="99"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003880.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="432" mass="48492" checksum="7D5285B03FEAF203" modified="2024-11-27" version="2">MKWNLFKKKPEAMVGPQPTGASAMTMAPLLSTTAENATESGEHVSKNDMFGDIKNKFLNEIDKLPLPPWAIIAISIVAVVLILTCCFCIVKKTCLKKKKGKKGKKGKGDMGMKNMKGGEKQDDDDDEDDIETGMTGDEKEEEEPKEKEKLGKLQFSLDYDFADNKLTVGILQAADLLSMDSGGTSDPYVKVFILPDKKKKFDTKVHKKTLNPVFNETFSFKIPFQEMGGKTLVMSVYDFDRFSKHDVIGEVKIPMNTLDLAQPIEQWRDLDSAEKEEPERLGDICVSLRYVPTAGKLTICILEAKNLKKMDVGGLSDPYVKIHLLQNGKRLKKKKTTVKKNTLNPYYNESFSFEIPQDQMQKIQAVITVLDYDKIGKNDAIGKIWVGSKAQGTALRHWSDMIANPRRPIAQWHPLRPEEEVDAELAGLTAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C1P3</accession>
    <name>A0A3B4C1P3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C1P3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006268.2">
        <property type="protein sequence ID" value="ENSPNAP00000004880.2"/>
        <property type="gene ID" value="ENSPNAG00000001647.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:beta-catenin binding"/>
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        <property type="term" value="F:cadherin binding"/>
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    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000095">
        <property type="entry name" value="Cadherin 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
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        <property type="match status" value="5"/>
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        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF78">
        <property type="entry name" value="CADHERIN-LIKE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="25"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="108"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="232"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="340"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004880.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="637" mass="70158" checksum="2161607BA39ACBE0" modified="2024-11-27" version="2">RRWVLSTIELQEEDPGPYPKELFNDKSSMHKLRFSISGQGVNEDPKGVFTIEPFTGAVIVHKPIDRELYPVFLVEFDVADRESGSILDRTLSFSVAIKDINDNAPEFLPEVLHTSIPENTQEGVLPVSLQAKDKDEKGNENSRITMRVVSQDPPSPVITLKPLSARGSDDNVITQLIFTGCFDYDVTKAYKLLVEAQDHGNPVLTSTATVNIDIIDSNTHQPVFTMETNKEILRLSVEDKDTPNTQASKAVYKILKGNEDGNYKIETDPKTNEGVLTVIKGKDYERTTLTQLEISVENEEPLFLCIDGKPASPAMRAARKLNTTKVDVKVIDVNDPPVFQQKIQTVYKKEEGNPGEVLYEPIVKDVDSDADKIRYELVDDPAKWVKVDPKTGKVTTVMKMDRESPYVVNGTYTVVIRAIDNGEPPATGTGTLVIQLGDLNDNAPYLTSNNSIMCGNKTQRVTVKPDDADAPPFAGPFSFSIASEDKKLLRSESSLISLKSLPYGNYLIPLRIEDQQGKVGLDTLNVVVCDCGDKDVCRSPLPRSSNLNGAAIGILLGSLLLLACKTKEFILNIQEEGNQTLINYNEEGGGSLAKVCLSSHITSISLKSVFLNLCKCLPKCCMMISIKITKTEQILSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C1Q1</accession>
    <name>A0A3B4C1Q1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lens epithelial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LENEP</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C1Q1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004890"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006248.2">
        <property type="protein sequence ID" value="ENSPNAP00000004890.2"/>
        <property type="gene ID" value="ENSPNAG00000001626.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441954"/>
    <dbReference type="GeneTree" id="ENSGT00390000002996"/>
    <dbReference type="OMA" id="RDMTMGL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029194">
        <property type="entry name" value="LEP503"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16968">
        <property type="entry name" value="LENS EPITHELIAL CELL PROTEIN LEP503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16968:SF2">
        <property type="entry name" value="LENS EPITHELIAL CELL PROTEIN LEP503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15221">
        <property type="entry name" value="LEP503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004890.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="6963" checksum="7CFBACF9B552B687" modified="2024-11-27" version="2">MHPQRPLPQAMPSSLGQNLRDAALGFGRGKNLLGGNIAYGVIQSLKECLYFLLCCWCIKEILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C1W2</accession>
    <name>A0A3B4C1W2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PLK4</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000004801"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006400.2">
        <property type="protein sequence ID" value="ENSPNAP00000004801.2"/>
        <property type="gene ID" value="ENSPNAG00000011183.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161268"/>
    <dbReference type="OMA" id="KMHYKRS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060030">
        <property type="term" value="P:dorsal convergence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060031">
        <property type="term" value="P:mediolateral intercalation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003307">
        <property type="entry name" value="KAISO-like zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000235">
        <property type="entry name" value="Zinc finger and BTB domain containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000437">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF94">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
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            <end position="26"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C905</accession>
    <name>A0A3B4C905_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PHD and ring finger domains 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PHRF1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_017722516.2"/>
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        <property type="nucleotide sequence ID" value="XM_017722517.2"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSPNAT00000000191.2">
        <property type="protein sequence ID" value="ENSPNAP00000008402.2"/>
        <property type="gene ID" value="ENSPNAG00000003007.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442472"/>
    <dbReference type="GeneTree" id="ENSGT00950000183205"/>
    <dbReference type="OMA" id="VENTRAC"/>
    <dbReference type="OrthoDB" id="1935339at2759"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16635">
        <property type="entry name" value="mRING-HC-C3HC3D_PHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15536">
        <property type="entry name" value="PHD_PHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PHRF1/Atg35"/>
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        <property type="entry name" value="SFR19-like_C"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618:SF20">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
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    </dbReference>
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    <dbReference type="Pfam" id="PF23030">
        <property type="entry name" value="SCAF11-like_C"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
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    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
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    <dbReference type="PROSITE" id="PS01359">
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        <property type="entry name" value="ZF_RING_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        </location>
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            <begin position="1827"/>
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            <begin position="544"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="571"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="606"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="654"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="676"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="706"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="740"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="760"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="817"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="860"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="930"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="953"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="986"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1028"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1057"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1067"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1092"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1252"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1263"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1319"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1341"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1469"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1490"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1527"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1699"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1731"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1744"/>
            <end position="1759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1845"/>
            <end position="1854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008402.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1854" mass="206220" checksum="7953A8972B73BAC9" modified="2024-11-27" version="2">MDEEDSQDELINKNASHGKGKRPASSILSDEEENSDDGCDSQEGATDSEEDDEDDDDASVDRDDEDGEEEDGEDSGHEDAEKDLEGAVGSTSTDLAGLSSDEDAEKCPICLNSFHEQPVATPESCMHYFCLDCILEWAKNANSCPVDRIVFSNILLRKCFGGKVQKTIMVQKTVKAGQEEQVDLDLEQTSCEVCGGSDREDRLLLCDGCDAGYHMECLTPPLDAVPVEEWFCPECAPNNHNTRRERSSEDESSILPTTSRSWLPTSRPTRAIARTQHSERVRANINRHRITQARTAAQIAPRYLMQSTWLDETINAVVAGLNTAVYVRDLTPRPRPRRRRRTVKRRKTKKEGKATGGKTASTGVKRRKRRVRRTKSRKKLVQKMKTTTRGRIARSLGIKKPVRSSSIPSVYRPSEQTLGSMRADIGAASLSIYGDPFDLDPFDDESEETQQAPTPSSLLEAKRRGLSRSALRSHQPVARPISAGLPSRRGQGIPQVQEVAEAAPVPDLLGSILSGQSMLLMDSSDVIINRDGSLKATKPVSLSLPRTSISGSSASEETRSTPSTEASPFKSARQPASSSLSPSAPPLQRRSSHSSPLPCPAPMTPVSPSSPTSLTPSPVLGQSRLHTPVPHRPVPSHPAGTEQSQWGTCAFRGQTPTRIQSGASDARCGVRGDAPQQPPAKKPPPKPVWLDVSGLPRIPKIKRESSSAVHGSSGSNGNNLPASSMTSLAGDKGREHTVDQQRASVSQSRAVDSQRHRLHSAGPSSSFSNSFSAPSSSASVTSNSRPSSSSAISFRISTSGNPWHARRLSAPEQALPVKDESAKKAQKDKQKLLTSHSQAKKPSVKSEIYDPFNPTGSDSSESEAEIDCPEMEPRSSVSMQPAVEQTEGPVHQIKAEPMDMDSPVEEEHSEMFVQVKNEPAPFKEDPWSLPSDQLASGHMQMQSGSLSAVMAYSESPAMSDSESSGTPESPFTKALGRSRSSSTSSHHSDNKVKMEVKAEPEDEPSPVQPKCKSEPDVPKGNGLCEVPSLASSSDVSSKSKQQEISPCPKPEEEGRQSESASQSPNSKELERCNKKNKHSRSEERRHSPSRSPCRRQSRSRSKEKRRSRSRSKEKRRSRSRSKERRRSRSRSKERRRSRSRSKERRRSRSRSKERKRSRSRSKERKRSRSRSKERRRSRSRSKERRQSRSRSKEKRRSRSRSKEKRRSRSRSKEKRRSRSRSRERRHSHLRDRRRSTRSSSNESSRKRKAKRDSHERYDGRLKDGHKRSKDKKRSRSHSQSRSRSRSRERRREQTSSHRSSSASRDGGEARPKRKRSRSTSRDRKRRADVPESSHQKERSTNKSTISTSVEATVPNHSLIKNALALKGKKDSGGNECETRISVVSVKVQSEPSSPGYSTLIEGSKNPEEITKETPLISVSNKPYEVKPKEIKQETIWPLDEVREPQLQIDIKEEREESYELCQELKNPEEIKIEDMTTTTEKSEEFSEISLSNASTPRNQTGYSLPQDEKSTNDTFDSSIQVKIEQIWPDDDDDDDGGGDDPYSDNALIINLCPADPAPMVCKKEPDGESPSLPVVAKEEMEVEPLQAPCLVKQEPVDSSDEDINVDYLIDNLDFIKKEMKEGSVTDPAGAVEPQTDLQEVSTESKQEMESSLVVAGTKAKSQGKRVTWNIQEPEGPQPEKMSKLALFKLKLKQEGSRKAASTSFTYDSSQESATVQVGAKPTVTSLNPPAQGGRSNSGQRGSKSHKEELLQDPQQKDKYMKKLHMQERAIEEVKLAIKPFYQKRDITKEEYKEILRKAVQKVCHSKSGEINPVKVANLVKAYVDKYKHARKHHKSGSSGQSQSVEDLKDSETPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9B1</accession>
    <name>A0A3B4C9B1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PEHE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017572840.1">
        <property type="nucleotide sequence ID" value="XM_017717351.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017572841.1">
        <property type="nucleotide sequence ID" value="XM_017717352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9B1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007810"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001209.2">
        <property type="protein sequence ID" value="ENSPNAP00000007810.1"/>
        <property type="gene ID" value="ENSPNAG00000002797.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439130"/>
    <dbReference type="CTD" id="567884"/>
    <dbReference type="GeneTree" id="ENSGT00530000063688"/>
    <dbReference type="OMA" id="DDMGAVP"/>
    <dbReference type="OrthoDB" id="6022640at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035035">
        <property type="term" value="F:histone acetyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026180">
        <property type="entry name" value="NSL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029332">
        <property type="entry name" value="PEHE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22443:SF19">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22443">
        <property type="entry name" value="NON-SPECIFIC LETHAL 1, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15275">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01300">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52052">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEHE" evidence="2">
        <location>
            <begin position="851"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="685"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="722"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="912"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="687"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="800"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="951"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="974"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1077" mass="118686" checksum="33A86770A263BF7E" modified="2018-12-05" version="1">MAAMAPALTDAPAEAHRIRFKLAPPSSTLSPSGVEGNGGTSHILVSSNGTVKRKASEERALRGAGSCKREEPARVDASQTHLGKIQPLVTSYRCSDVISMHSPKESLKLQGVLLQNHSILPGFLPRKHQALELSEEQFKSIMSGGSGSGPCGPPALQGTPVNGMAKKLTKPGPDNRENTLTLNGGRHAGQESLLQSQVVASHRGLMGNGPLRGGTEQHQCTRDVSQDHPSNQESLSSFSPSIAPSDLDEEKVSDRLPSLTTDTSVVSPAAPQYPLGSVGAELRDRTQQSQSRQGEIEGRLRRLRKRLQVVQAKQVERHVQQQLGGLLRSTLGPLDALRQQQGRHSTEPLTPQERDRLGRFLKGGSVPAELERLSLNCTTNLRSAENAFDSDATESSSGGETDVEEDELARVDIEQRYIPLWRRAEGRYAVDRASIISHWNWLQAHVSDLEYRIRQQTDIYRQIRANKGPVVLGESSVCESVAEDSGLSRTDTISWPASQARDVDWTEGSVSAYGIAMETNLRKGCAPVRQVNGVINSLRASSPESAELEEQLRLQQRSSSVPDSSCVAARSRPLLSCKRRRLLRPSSITNLNRKVQKQAGTGRCGCEVSGQCVMCSGRALAPVDAQFQLPLLDRLAQFDPCVHPILSFSDDVGISLHLQRVMKCHWQGRSLEKIKPIKKLSLKHKLPLSSRLPEPSPSSSSKDKHKLTNSLLSAVRLSHHKIRPEKLLRQQSDGGESRQHFRPERSHTAFDKSHSRKRPREHSLERTENAPKLYMEVSSPCPSLPSLNTPIHSPLVRQLSSSESPSPCSLHNTTPQPIRRRRGESSFDINNIVIPMSVAATTRVEKLQYKEILTPSWREIDVTAKTISEEDDSVEIEDLSDAVFSQLHFTNEEQERSRWSWTASAIAKRRGSRSYKSVDGRTTPLLGGTNPSTPQPSSPDTAHFHLLQDYSSATSPSSPASPELLMFSGLHTPGPRDSHRLLSNEDTRCSTPDTIYEEMVSQPVQPWERRTFPLDIDPELEPKDQSSPASERSCRTMRRISGCKTGSSKSESDAGPPSPLPEDNSKQKATPHKHTHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9F6</accession>
    <name>A0A3B4C9F6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540733.2">
        <property type="nucleotide sequence ID" value="XM_017685244.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9F6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008263"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000433.2">
        <property type="protein sequence ID" value="ENSPNAP00000008263.2"/>
        <property type="gene ID" value="ENSPNAG00000003010.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412979"/>
    <dbReference type="CTD" id="798983"/>
    <dbReference type="GeneTree" id="ENSGT00940000156972"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
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        <property type="term" value="C:apical plasma membrane"/>
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        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
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        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
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        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
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        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
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    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
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    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="488"/>
            <end position="510"/>
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            <begin position="124"/>
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            <begin position="459"/>
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    </evidence>
    <sequence length="1116" mass="125971" checksum="107568D17DB75B1F" modified="2024-11-27" version="2">MDIKDQGAQMEPLLPPVQSPVSSEERNDEEAVLDRGGTRSMLNTNFEKEELEGHRTLYIGVHVPLGRRSHRRHRHHGHRHRKRSRERDSGTEDGRESPSYTDTPSQRVQFLLGMEDEDEEHIPHALFTELDEICLREGEDAEWKETARWLKFEEDVEDGGERWSKPYVATLSLHSLFELRSCILNGIVMLDMRANSLEEIADMVLDQHETSVAVGEEVRRKIRNALLKQHHHQNQKKLANRIPIVRSFADIGKKQSEPHSMDKNGQMVSPQSQPSCTEARSDGSRENSAVDFSKIDLHFMKKIPPGAEASNILVGELEFLEKPVVAFVRLSPAVLLNGLAEVPITTRFLFILLGPLGKGPQYHEIGRSIATLMTDEVFHDVAYKAKDRSDLVAGIDEFLDQVTVLPPGEWDPTIRIEPPKNVPSQEKRKIPPLPNGVTDLGEAEAHGGHGGPELQRTGKFFGGFFLDIKRKAPHYLSDYTDAISLQCLASFLFLYCACMSPVITFGGLLGEATEGRISAIESLFGASMTGIAYSLFAGQPLTILGSTGPVLVFEKILFKFCKEYGLSYLSLRVCIGLWTAFFCLLLVATDASSLVCYITRFTEEAFAALICIIFIYEALEKLVHLGELYPFNMHSDLNKLTQYSCVCTEPKEPSNATLRYWQEKNITSSEVDWLGLDVKGCVQHRGEFKGSACGHHGPYIPDVLFWSVILFFSTVAMSSFLKEFKTSRYFPTKVRQIISDFAVFITIVSMVGIDYALGVPSPKLQVPNEFKPTRDDRGWLISPLGPNPWWTIIITFIPALLCTILIFMDQQITAVIINRKEHKLKKGCGYHLDLFVVGVMLGLCSVMGLPWFVAATVLSISHVNSLKLESECSAPGEQPKFLGIREQRFTGLMIFVLMGCSVFMTSVLKFIPMPVLYGVFLYMGASSLRGIQFFDRLRLYGMPAKHQPDFIYLRHVPLRKVHLFTIIQLSCLVLLWVIKTSRAAIVFPMMVLALVFIRKLLDFIFTKRELSWLDDLMPESKKKKLEDAQQEENQSVMVEDEGIVQVPLEGHYKDDPSTVNISDEMTKTSFGNVWKSLNTDNGKKDQSAKSSPTEDIRRSEKPEEFKDRDLDRETSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9H1</accession>
    <name>A0A3B4C9H1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tetraspanin-10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Oculospanin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TSPAN10</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with ADAM10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9H1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007556"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001628.2">
        <property type="protein sequence ID" value="ENSPNAP00000007556.2"/>
        <property type="gene ID" value="ENSPNAG00000013335.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160874"/>
    <dbReference type="OMA" id="CLDPVPW"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03167">
        <property type="entry name" value="oculospanin_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000033">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF550">
        <property type="entry name" value="TETRASPANIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083961"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007556.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="346" mass="38130" checksum="F8973F2AE556BFE7" modified="2024-11-27" version="2">LSRISFWSEETPQTRPASSLTYLGTDDSSESKTDSSSSSHEEQSRMFLRPQTHTISSLTDCLLKFVLFSSNLLFTALGLATLGLGLWGLFNKESFTQEKLDSIGTDPMLLFVFLGLVLSLLCLTGCVGAVRENCCLLRTFSVVLLFLVAAQVLAAIVAYSLQGQITNLLRTGMLNAMVRYQDDLDLRFITDEIQTGLQCCGADNYRDWQINSYFNCSAPGIQACSVPPSCCVDPLENGTVWNSQCGVGAQLLDEFSAQSVIFLGGCLGSISRWIEQHSGLIGTVAIVLLGVQLIMLIVATRLLDNIQWNRTQAHNENFLGPYFSWTPSQPPQRLVNIINYIMSSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAM7</accession>
    <name>A0A3B4CAM7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="6">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">GCH1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAM7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007936"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001004.2">
        <property type="protein sequence ID" value="ENSPNAP00000007936.1"/>
        <property type="gene ID" value="ENSPNAG00000013606.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013481"/>
    <dbReference type="OMA" id="RENLHGE"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF11">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="19">
        <location>
            <begin position="142"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="320" mass="35903" checksum="7AE1FC50648B741C" modified="2018-12-05" version="1">MFIKPNVLSAPEWSFYLLWKTEEDRRSEKSHRFSVRADHSLSLSLSLSLSPSLSLSLSLSLSPLDMERSKVKQSEKEAPVGSDASLNGHCEGGRAKVAKWKDAPGEACSPSSSKQTSSVMESWKEERTRSVEDNEMSLPSIAAAYTTILRGLGEDPQRQGLLKTPWRAATAMQFFTKGYQEKIIDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPIFGRVHIGYLPNKRVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALQPAGVGVVIEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTRDEFLTLIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CHS6</accession>
    <name>A0A3B4CHS6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L1 transposable element RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CHS6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035905.2">
        <property type="protein sequence ID" value="ENSPNAP00000010905.2"/>
        <property type="gene ID" value="ENSPNAG00000016510.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171226"/>
    <dbReference type="OMA" id="KCEDMEG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.250.20">
        <property type="entry name" value="L1 transposable element, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042566">
        <property type="entry name" value="L1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="141"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010905.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="39465" checksum="2E241D6FD963C7F0" modified="2024-11-27" version="2">QSRGASEPRRSRDEYFRAPSLKVSNKLRKFKYDCNTSKLAASTRSTRQRTLEENDPQRKSSGMSEEVKAEILASIRGDISKIIREEIRGALSEDLDALKADIKAARAEIASNATTTRAEITSMKTDIEDVKSGLSTWSDEVSTLQTTVTELQSELAKLRDKCEDMEGRMRRGNIRIAGIDERPNSASPNEVSKMIKEVLKMDRDVKVDRSHRTSTSKKPRDSPRVIIAKLHNDGDAADILRRARDHAPLTYNGKRIAIFPDYTASVTKARAAFTDARKALRGRGDVRFGLLYPARFKITYKEDSKEFRDPIKLPFQLIIVSMNALFFHNGKFQEKCSTVLRLTTPLMYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJ05</accession>
    <name>A0A3B4CJ05_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">AKAP10</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017554643.1">
        <property type="nucleotide sequence ID" value="XM_017699154.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJ05"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011350"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036091.2">
        <property type="protein sequence ID" value="ENSPNAP00000011350.1"/>
        <property type="gene ID" value="ENSPNAG00000017025.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428276"/>
    <dbReference type="CTD" id="11216"/>
    <dbReference type="GeneTree" id="ENSGT00390000015077"/>
    <dbReference type="OMA" id="EFHCKYQ"/>
    <dbReference type="OrthoDB" id="5584247at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12804">
        <property type="entry name" value="AKAP10_AKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08735">
        <property type="entry name" value="RGS_AKAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08721">
        <property type="entry name" value="RGS_AKAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000018">
        <property type="entry name" value="A-kinase anchor protein 10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000019">
        <property type="entry name" value="A-kinase anchor protein 10, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000005">
        <property type="entry name" value="Putative A-kinase anchor protein 10 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037719">
        <property type="entry name" value="AKAP10_AKB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052246">
        <property type="entry name" value="Cell_Polariz_PKAAnc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155:SF1">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155">
        <property type="entry name" value="A-KINASE ANCHOR PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="101"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="356"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="638" mass="70206" checksum="E82DFDF93DD45DFC" modified="2018-12-05" version="1">MAFFRRKAKGKELERAADAKAGKAPVSPNSPSRNHHAILEAAGPSHVAINAISANMDSFARGRTAILKKQPSHMEAAHFGDLGRSSVNYQAQETRSRLSKTVEQILHDGVALPYFLQFMETRSAEHLVRFWLEAESFRSASWSRVRAHSLNSVKHSSLAEPASAIGSPGSPDSPSALSASPGVDGGESEDPFPSRIPSSPQDTNSNGGAGTGHRNSICCEGGQRPATPQQLSLRVETPTRLSSVSSRSGTPVKGQSTNALRELSDKLMKSIERDAVNIFTKYISPDAARPIPITEQIRNDIVAKICGEDGQVDPNCFVIAQSVVFTMMEQQHFSEFLRSHYFCKYQIEVLTSGSVFLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQVQLAAKKGQYDGQEAQNDAMILYDKYFSLQATNPLGFGDSVRMEIESNICREGGPLPDCFTTPLRQAWTTMEKVYMPGFLSSNLYYKYLSDLINSVRADEFVGGNSSMSGHSTTPDSDRGSNASEGSQAQQGTKKAVIKILKNFDEAITVDIASLDPESLYQRPYAGRMTFGKVNELGQFIREAEPEPDIKKSKGSMFSQAMKKWVQGNSDEAQEEMAWKIAKMIVNDVMQQGQHESPVAKSTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJK1</accession>
    <name>A0A3B4CJK1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCA7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017566691.1">
        <property type="nucleotide sequence ID" value="XM_017711202.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJK1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010694"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017441.2">
        <property type="protein sequence ID" value="ENSPNAP00000010694.1"/>
        <property type="gene ID" value="ENSPNAG00000016270.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435384"/>
    <dbReference type="CTD" id="692316"/>
    <dbReference type="GeneTree" id="ENSGT00940000159736"/>
    <dbReference type="OMA" id="WLFKRTH"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052237">
        <property type="entry name" value="Ataxin-7-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117">
        <property type="entry name" value="ATAXIN 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117:SF5">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="2">
        <location>
            <begin position="258"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="463"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="529"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="760" mass="82083" checksum="9C79EE22EE7E2668" modified="2018-12-05" version="1">MMAVRERAVKVMAALDRRVPCLDDFVGQSWSVWAERTSLTAPDGSDGEDCSKNGKKTTETMTLRKEDMSIFGHYPGHDDFYLVVCSHCGQVVKPQAFEKHYERRHGSVGKLYAHMRATPPAAQQRTHPRHGHTPPATGHGTASWSGRNQGAGGPPRPAPQSPSTPPQFRHSKTTKDAVRLSPLEKTSNSGHSESSVFKQPPPLEPPRNSPPPSLRDPPWPHGGPSPSRPVTTERSPSQRAEPSSAPATPGHARGTRPHKVSKKECDLDKHCGVLDPERKKVCTRLLTCNIHSVHQRRKVVGRSKNFDQLVAELKMGSKSRERMAQTHEGSAALSPSLEAPREPTNSPHCRRPLANSPAFRTRTLSESTPEEEKAVREEGGARASSPLVQGRISSDESEGEAAEDPPDWHSTPWHPKPLAYCTFGSCALGHGVFTFDRRLHHLRSAVSTMVESHLSAHLWKKIPQASDPQSQSPSAKPSTVPASPSSTSHHSKPKAGNHNSASVKTSLYSSQGPGKEAHPQSSSTSRTYGQSEKSGGSSQSAAAHSKPARTASQPGGPGRPKNPVGRPSKQQVRLREVERVTPASALRKRKASVGEADAVSPDRNCLSLDRDRDRVRPPVSSKTSPAPNPTPAPDRPTPPSHGQTNGSLSPGHKPPRPQLGPSSAESPHGPSAWPFKRTHTSPPDPHGRGGNSGHHSRVPGYEHRGLGKKRKSNDSSPPTKAHRLPTSPHSGFYPWKESKGAALPVGGEKKLSAPKPKLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CLT4</accession>
    <name>A0A3B4CLT4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="11">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CLT4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019534.2">
        <property type="protein sequence ID" value="ENSPNAP00000012313.2"/>
        <property type="gene ID" value="ENSPNAG00000018085.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230412"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000297">
        <property type="entry name" value="Uridine-cytidine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012313.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="262" mass="29855" checksum="F75EA852C4DE1F57" modified="2024-11-27" version="2">IMAGDSETRLNGQADDTNTVKQPFLIGVSGGTASGKSSVCEKIMELLGQNKIDHHQRQVATLSQDSFYRELTPEQKAKALKGQFNFDHPDAFDNELIMKTLHDIVQGKTVHIPVYDFVTHSRKDEYVTLYPADVVLFEGILMFYTQEIRDLFQMKLFVDTDPDTRLSRRVLRDISERGRELEQVLSQYITFVKPAFEEFCLPTKKYADVIIPRGADNLVAINLIVQHIQDILNGGFSKRQNGYVNSHGTPRQRRPSESSRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMV1</accession>
    <name>A0A3B4CMV1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15 19">Postsynaptic cell membrane</location>
            <topology evidence="15 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 19">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580652.1">
        <property type="nucleotide sequence ID" value="XM_017725163.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMV1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020033.2">
        <property type="protein sequence ID" value="ENSPNAP00000012683.1"/>
        <property type="gene ID" value="ENSPNAG00000018493.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444168"/>
    <dbReference type="CTD" id="100151589"/>
    <dbReference type="GeneTree" id="ENSGT00940000163942"/>
    <dbReference type="OMA" id="GPFNYFE"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13685">
        <property type="entry name" value="PBP2_iGluR_non_NMDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000143">
        <property type="entry name" value="Probable glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000364">
        <property type="entry name" value="Si:dkey-183j2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000398">
        <property type="entry name" value="Si:dkey-183j2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 19" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7 19" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 19" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027166548" description="Glutamate receptor" evidence="19">
        <location>
            <begin position="29"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="21">
        <location>
            <begin position="32"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="22">
        <location>
            <begin position="42"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="455"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="17">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="17">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="333"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="467" mass="51282" checksum="F521A445147A0028" modified="2018-12-05" version="1">MVIFNNMWTLLAFTVSTTVLLLTHSCTAAKSELIVTTIKQDPYTMAKGSQLEGYCMDLLSELAKKLGFKYKVHLVKDGAYGRMDDSGSWNGMIGEVVRGEADIAVAPLTLTAAREKAVGMTKPYMQTGISILLRRDIVSQDSGFFDFLSPFSGETWFGILIAYILTAVCICIIARLSPSEWSQPENEHNNFTLLHSLWYTAGALSLQGAGPHPKSPSGRVVSCTWWLFAVVLLACYFSSLSSNQSSDSSHLMIKGFEDLANQDFIEYGTLTGSSTLAFFKNSNNPSYRRIYEHMERRKSLVSSMDEGVQRAREGNFAFIGESVSLDLAVARYCELVRAHEVIGMRGYSIVTPLGSPLLKNLSVAILQLSEAGELAYLRSKWWASSCMAHSAKASSLKAHGMKGIFLVLAVGLGIGVLLALLELTSKSRSSAQEQKKSCCSVLTQELSQRLRTSNTKRDQETSDKSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CN03</accession>
    <name>A0A3B4CN03_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563640.1">
        <property type="nucleotide sequence ID" value="XM_017708151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CN03"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012196"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020352.2">
        <property type="protein sequence ID" value="ENSPNAP00000012196.1"/>
        <property type="gene ID" value="ENSPNAG00000017934.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433527"/>
    <dbReference type="GeneTree" id="ENSGT00940000156303"/>
    <dbReference type="OMA" id="ANMNVYL"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01469">
        <property type="entry name" value="vWA_integrins_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000012">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF23">
        <property type="entry name" value="INTEGRIN ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017101810" description="VWFA domain-containing protein" evidence="16">
        <location>
            <begin position="25"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1131"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="169"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="361"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="419"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="471"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="534"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="596"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1184" mass="129348" checksum="D08BA91ECCF3C31A" modified="2018-12-05" version="1">MDLISSLLLVFLTVQGWCVEQIWGFNVGTAGAKVFSGAALEEFGYTVQQFSNYQGKWLLVGSPWAGYTQNRKGDVYKCEIKSPGSTCEKLNLQNFVNIPTVQNINTNMSLGLTLTRITKNNGFMTCGPLWTQSCGTQYFVPGVCAEVSPQFYAQPAFSPALQTCGGPMDIAFVVDGSNSIYPWEPVVAFLKKVLENLDIGPQKTQVSIIQYGSDPRFEFYMNAYQTKQAMIAAASKIAQKGGLETNTFGAINFARTNAFLTSNGGRPGASKVMVVVTDGESHDNSLTDKVISQCNKDGITRFGIAVLGYYTRNNIDTTKLIAEIKSIASIPTEKYYFNVSDEVALSEIAGTLGDRIFNIEGTGKGGEFQMEMSQVGFSAHQTKNQDVLMLGAVGAYGWSGTVVHKSAQKSDIFPKHAFERILEDKNHSSLLGYSVTTLNDGSSEFYVAGAPRSNHTGQVVVYTLDSQRQPNIIDSQRGDQIGSYFGSVLCPLDLDHDGVTDLLLVGAPMYMSDQKKETGRVYIYSITIGVLSSQGFLEGSSPLENARFGMAITAVPDLNLDGYNDVVVGAPLEENDQGAIYIYNGDQRTIRKQSSQKILASKLDPSLKFFGRSLDGNTDLNDDSLPDVSVGSYGKVLQLWSRGVAVVTAKVTFTPDRISILNKPCSFGGRMVFCFTAKICFRAAFRPTNPVGPAEIKYNLTLDADLQSSRVSSRGQFSNSDRVVQRDISVSTKDTCEDHQVYVQEAPDLVNSIALRVDIKLRHPDANPVLDAFSPTAWEFFIPFSKDCGSDEVCYSDLVLSVEREPKAGSFLVTHKTKRLSFTVTVMNRKENAYNTRVSAHFSSNLFYSSVTPPADGTEVKCTSAKDTEVLTCQVGYPAIRTDQSVTFEINFEFNLGQLQKDVQIVFEAQSDSTEETPADNKETVSIPVQYSSEIILSRDANMNVYLLDNDGGITTTMKTYSDIGPEFKFSLKVSGGIFPVSLVYLNVSLPSSTKAGNPLIYITSVTTAPAGDVKCETSTLTDPLKIKEKEYTAKFTKESFRGTTELNCKTATCETMKCVLKDVNPKSDYYVNVTAWIWSGTFAMADFQSVVLSSNAQIETFQPDLLIITHKQAQVAVTVSKPGAKADVPVGVIVGSIIGGLLLLALAVAALWKVGFFKRKYQQLQKNDDGPETEGLQESATQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPZ6</accession>
    <name>A0A3B4CPZ6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein kinase (cAMP-dependent, catalytic) inhibitor gamma</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PKI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPZ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013425"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021059.2">
        <property type="protein sequence ID" value="ENSPNAP00000013425.2"/>
        <property type="gene ID" value="ENSPNAG00000019275.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002707"/>
    <dbReference type="OMA" id="TRDSHIF"/>
    <dbReference type="OrthoDB" id="8556393at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004171">
        <property type="entry name" value="cAMP_dep_PKI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15416">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE INHIBITOR/PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02827">
        <property type="entry name" value="PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013425.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="76" mass="8308" checksum="2D0FE1268D0AE668" modified="2024-11-27" version="2">MMDVESLYSDFINCDRTGRRNAVPDIKGEGTAGGTSELTKDMAQMDLKAGGRSASDTPIFGESVRTQQKCMLFKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRL6</accession>
    <name>A0A3B4CRL6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539558.2">
        <property type="nucleotide sequence ID" value="XM_017684069.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037388615.1">
        <property type="nucleotide sequence ID" value="XM_037532718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CRL6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013988"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021766.2">
        <property type="protein sequence ID" value="ENSPNAP00000013988.2"/>
        <property type="gene ID" value="ENSPNAG00000019902.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412151"/>
    <dbReference type="GeneTree" id="ENSGT01030000234599"/>
    <dbReference type="OMA" id="HMAVAIT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF28">
        <property type="entry name" value="APOLIPOPROTEIN L, 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05461">
        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013988.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="313" mass="33516" checksum="C0B2B63D16750671" modified="2024-11-27" version="2">MACSNDHRRFDDDSDSSGSADSNRRMEQPPSAELGVPPDESLMAKIHLYTSLFDSSVSDLKAQISKLTEITDNLEKTHRNATIGSLSGGVISATGGIASIVGVCLAPFTLGVSLIVTGVGVGVAVAGGVTGAASNFTDMAQQRKSREEIEEILKAFQKKISPIIKCLEGIVALLDKIQRAEFSESPVQKVQAIFGVSRGAGHAAELLRLVSFVEIGKVAAQVSRTLRVAAAMTGVISGLLLVFDIVFITKDVNELIEIDKKQKDGSEKSDVVKFIQEMRKTADDLKQTLKELKTHKDTLNAEITDKLKETSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CUS9</accession>
    <name>A0A3B4CUS9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Charged multivesicular body protein 4b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017542998.1">
        <property type="nucleotide sequence ID" value="XM_017687509.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CUS9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015155"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023321.2">
        <property type="protein sequence ID" value="ENSPNAP00000015155.1"/>
        <property type="gene ID" value="ENSPNAG00000021179.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414620"/>
    <dbReference type="GeneTree" id="ENSGT00940000154663"/>
    <dbReference type="OMA" id="KIDHELM"/>
    <dbReference type="OrthoDB" id="5592979at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1060:FF:000001">
        <property type="entry name" value="Charged multivesicular body protein 4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF14">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="174"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="87"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24708" checksum="77479779AA6289A6" modified="2018-12-05" version="1">MSVFGKLFGSGGKGGKGPTPQEAIQKLRETEAMLTKKQEFLEQKIEAELLTAKKNGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKNAHQNMDIDKVDDLMQDITEQQELAQEISDAISRPVGFGEEFDEDELLAELEELEQEELDNTLLEIGGTENVPLPSVPSTSLPSKPAKKRVEEDEDEMEELKAWAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVD0</accession>
    <name>A0A3B4CVD0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS10m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">28S ribosomal protein S10, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRPS10</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566188.1">
        <property type="nucleotide sequence ID" value="XM_017710699.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVD0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014701"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022692.2">
        <property type="protein sequence ID" value="ENSPNAP00000014701.1"/>
        <property type="gene ID" value="ENSPNAG00000020655.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435110"/>
    <dbReference type="CTD" id="55173"/>
    <dbReference type="GeneTree" id="ENSGT00390000009045"/>
    <dbReference type="OMA" id="IRWVQPA"/>
    <dbReference type="OrthoDB" id="366214at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000005">
        <property type="entry name" value="28S ribosomal protein S10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040055">
        <property type="entry name" value="Ribosomal_uS10m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13334">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13334:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US10M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="8">
        <location>
            <begin position="78"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="202" mass="22923" checksum="50368E6FC1C14022" modified="2018-12-05" version="1">MTASMRIASVRLLSKTRHYFSSEVAVQPGARTVHHQPASTRNVCLIPVAFLHKGRAYGTQSSSVTVTEEPDLLFQKLSVLVKSHDKAVLDSYEFFATLAAKELGLTLAKVFEPPKNIERLTLLKSVHIFKKHRVQYEMRTHYRCIEISRITGSTSRVYLEYIQRNLPEGVAMEVTKTAIEKVPDHIQMPLWSVQSQEKQGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CW08</accession>
    <name>A0A3B4CW08_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TPD52L1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CW08"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015550"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023897.2">
        <property type="protein sequence ID" value="ENSPNAP00000015550.2"/>
        <property type="gene ID" value="ENSPNAG00000021604.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="OMA" id="YCIRTET"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="135"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="266"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015550.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="296" mass="33005" checksum="CE21BC8BB37C06CE" modified="2024-11-27" version="2">TEQLPHLNPLVVSAAPGETVTLNCPSVGDSDADVRMWYKQRLEHAPLEVGSKFKTKDSIISAPFDKSRFKIERTAQNVSLSIERVTKGDEGMYFCAAAGEKTMNFSTATFLAVTGSRTILLYQLFLTWGSVSPRESVTLQCTVLSEIRAADLRVLWFRAAAGQSFPEIIYTHQNSSSRQCEISSSTSCSLYEFSKNILDHHHTGTYYCAVEACGKIIFGNGTSVELSKYFFALSKLFFKLSCLFLQDENAEELNCAALNFHERKTKRGRGKREQPQEDSLYSAVRGSSVNDCRATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0D4</accession>
    <name>A0A3B4D0D4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580507.1">
        <property type="nucleotide sequence ID" value="XM_017725018.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0D4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017055"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025709.2">
        <property type="protein sequence ID" value="ENSPNAP00000017055.1"/>
        <property type="gene ID" value="ENSPNAG00000003763.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444050"/>
    <dbReference type="GeneTree" id="ENSGT00940000161090"/>
    <dbReference type="OMA" id="QRANLED"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF9">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="80"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="84"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="282"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="439" mass="49731" checksum="074460EE66918C43" modified="2018-12-05" version="1">MSLRSKRISSSSSVRSSGKLGGFGYSGGFSGMSLGASSPGFSTSSTYLGAPIGSVSVNKSLLAPLNLEIDPNIQMVRTQEKEQIKSLNNRFASFIDKVRFLEQQNKMLETKFDLLQCQSPGRSNIEPMFEAYMANLRRQLDVVNNDKIKLDGELRNMQGLVEDFKHKYEDEINKRNNLENDFVILKKDVDSAYLVKADLEDKVGALTDEINFLRSIYDEELREMQASIKDTSVIVQMDNTRSLNMDQIVAEVKAQYEDIAAKSREEAESWYKSKFDQMASQASQYGDELRNTKGEIAELNRMISRLQSEIEAVKAQRANLENQIAEAEGRGELAVKEAKARIRDLEEALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEEDRLGQQAIVNIQSMPNYNAKGMNGYQQMSSSSPKILIKTTETRDNTRFSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3K4</accession>
    <name>A0A3B4D3K4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">High affinity receptor for melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibits adenylate cyclase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559587.1">
        <property type="nucleotide sequence ID" value="XM_017704098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3K4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017459"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026253.2">
        <property type="protein sequence ID" value="ENSPNAP00000017459.1"/>
        <property type="gene ID" value="ENSPNAG00000003779.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431134"/>
    <dbReference type="CTD" id="565718"/>
    <dbReference type="GeneTree" id="ENSGT00940000160321"/>
    <dbReference type="OMA" id="YTISIEC"/>
    <dbReference type="OrthoDB" id="10044919at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008502">
        <property type="term" value="F:melatonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000056">
        <property type="entry name" value="Melatonin receptor type 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002278">
        <property type="entry name" value="Mel_1A/1B_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000025">
        <property type="entry name" value="Melatonin_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF53">
        <property type="entry name" value="MELATONIN RECEPTOR TYPE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01149">
        <property type="entry name" value="MELATONIN1AR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00857">
        <property type="entry name" value="MELATONINR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="46"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="352" mass="39883" checksum="76E8FAE40E215543" modified="2018-12-05" version="1">MKTNGSRLTNTSASLAPSELTLTRPPWVSTTLGSVLIFTIIVDILGNLLVIFSVYRNKKLRNAGNIFVVSLAVADLVVAVYPYPLVLMSIFHNGWNLGFVHCQISGFLMGLSVIGSIFNITGIAINRYCYICHSLKYDKLYSDKNSLCYVLLIWILTVVAIVPNFFVGSLQYDPRVYSCTFAQSASSAYTIAVVFFHFILPILIVTYCYLRIWILVIQVRRRVKPEFRPKLTPHDVRNFVTMFVVFVLFAVCWAPLNFIGLAVAVDPVRVAPLIPEWFFVSSYFMAYFNSCLNAIVYGLLNQNFRREYKKIIVSFCTARMFFPESSNEGVDRIKSKPSPLMTNNNQVKVDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3N2</accession>
    <name>A0A3B4D3N2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pseudouridylate synthase PUS7L</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pseudouridylate synthase 7 homolog-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PUS7L</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Pseudouridine synthase that catalyzes pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573302.1">
        <property type="nucleotide sequence ID" value="XM_017717813.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3N2"/>
    <dbReference type="SMR" id="A0A3B4D3N2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017489"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026301.2">
        <property type="protein sequence ID" value="ENSPNAP00000017489.1"/>
        <property type="gene ID" value="ENSPNAG00000023742.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439411"/>
    <dbReference type="CTD" id="83448"/>
    <dbReference type="GeneTree" id="ENSGT00530000063554"/>
    <dbReference type="OMA" id="FPNNKVH"/>
    <dbReference type="OrthoDB" id="447290at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.20:FF:000005">
        <property type="entry name" value="pseudouridylate synthase 7 homolog-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056963">
        <property type="entry name" value="PUS7L_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056961">
        <property type="entry name" value="R3H_PUS7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23943">
        <property type="entry name" value="PUS7L_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25094">
        <property type="entry name" value="R3H_PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRUD" evidence="8">
        <location>
            <begin position="373"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="651" mass="72566" checksum="C6B926F9E83576D1" modified="2018-12-05" version="1">MERDTQPCFMSDHEGFCGSIKNSTRDFVVTELDVQGRPVTSTGRPEVAEVRQGSRKAGTAGVTTVVQNGDGVLSQEGFDLSAILGSSVHEALGGFASRTVACVEEQKEDTEMALGTFPEKQQRASVHRAVRHNYPFLMTVTRQTGILVKVDPDFRELSQLVSEGEAEDFFRFIDAKVPGSVFTFLPDDSKEHRTSVHHFISRRFGKLVETKSFAEQDQTAIAVRLREKGNQGKKSGKRSASDREEQAEIYSGFTLKKENLETLEAISYMAAVLGVLPSDFSYAGIKDKRAITYQSMVVKKISAERLLEKASEFERRGMQLSNIHPASEPLRLGRLQGNHFDLVIRDLKPHGKCSSSDLEALVKAAVENVKARGFVNFYGPQRFGNSQTVQADQVGLALLKEDMVTAVRLFLSPEEGEDLQNKAKRHFLRTGNAKESLLLMPAYKARERLMLRALHRYGSGQEGCGRAWLSLPHGMRVFYLHAYCSRLWNEAASYRLNTLPHWPVQGDLVCTRPQRDASATEESDAAQVHVVTHAEEKDNAFSLKQVVLPMPGNSVKYPENVLGKWYQDRLAQDSLSSCRFRITSLKLNVPGCYRPLLAHPHDVTYSLCVKEEPQVTGPSPPHSTASLTLSFDLDSSCYATVCLREIMKCEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3Y4</accession>
    <name>A0A3B4D3Y4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger transcription factor Trps1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TRPS1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017552115.2">
        <property type="nucleotide sequence ID" value="XM_017696626.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552124.2">
        <property type="nucleotide sequence ID" value="XM_017696635.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552134.2">
        <property type="nucleotide sequence ID" value="XM_017696645.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552141.2">
        <property type="nucleotide sequence ID" value="XM_017696652.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391393.1">
        <property type="nucleotide sequence ID" value="XM_037535496.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391394.1">
        <property type="nucleotide sequence ID" value="XM_037535497.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000018260"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027414.2">
        <property type="protein sequence ID" value="ENSPNAP00000018260.2"/>
        <property type="gene ID" value="ENSPNAG00000024647.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426840"/>
    <dbReference type="GeneTree" id="ENSGT00940000157893"/>
    <dbReference type="OMA" id="YESCHSM"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000020">
        <property type="entry name" value="Zinc finger transcription factor Trps1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028440">
        <property type="entry name" value="TRPS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47034">
        <property type="entry name" value="ZINC FINGER TRANSCRIPTION FACTOR TRPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47034:SF1">
        <property type="entry name" value="ZINC FINGER TRANSCRIPTION FACTOR TRPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="13">
        <location>
            <begin position="869"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="438"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="838"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="927"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1023"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1142"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="734"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="936"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="950"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1033"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1049"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1178"/>
            <end position="1208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018260.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1295" mass="140076" checksum="9FCB485B8F0E5747" modified="2024-11-27" version="2">MLSRDMVRKKNPPLRSFGGEQEEGEVVAAEATGSESGEVGSEASEPDRPMAEEQGEPGSPGPINADKPGFHYAGPADAVALNPATSPQQSDPGEAGDQARSPAGEGEVTKAGVEGQRPGCIIVQGCTTSNGDERDPTHSPVVLLGSDVLHGDGVDHSKLPPVPLHPRRPSDAPEEASSPLGAETGAPPASSPKLQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFMGQTQTQAKDGSRAAALNAGGGAGVEAGSPRASLLNGTYDVQVTLAGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGSSSVELEQHFLAAHPNKMKNPPPASPEEKVSERKGNCLSWQGSENQGTWADRVAVRAEDDSVAGYSVPIQPPDSQGHPAGDTPAAYYWCKYCSFSCEASGSSRLLEHYEKRHGQAAVREGSPGERERKSQDRGDAHVRRKENQCGKGDPDSMVTSYNCQGGKGEPDTVVTSYNCQFCDFRYSMNHGPDVIVVAPLLRHYQRAHSIHKCTIKHCPFCPRGLCSPEKHLGDISYPFACRKSSCSHCALLLLQLTAAGGSSTPPPTARASVTHLCDQCPFATTDIDLLLLHYDSAHAPHTLLDVKAEEETTGEGRGQQGEYACTKCHFYTEVEEEIFRHYRRVHGCCRCRHCSFTAADTTALLDHFNSAHCQDPGDPSSAPANGCSAPSTLRIKEESKGDLKLYSLAPPEAKPAEAVKSEAPDEKEKERACSEALGPGARGTGRGAEQAVPGLVWVPKERAAEVLRGSPAQFQTQGALGLLNAVQEQQPQKVLRDSPSLMFSLSTDAKGYLQGTPASVAEKPGQQYPTFTESKSAKEESQSLLRRRRGSGVFCANCLTTKTSLWRKNANGGYVCNACGLYQKLHSTPRPLNIIKQNNGEQIIRRRTRKRLNPDSLPSEQVGSKQQRVNSEERLNGSPLERRPDEPGADGPLPRADSAQAQLTKYEGYGPSGPKGHPSPRSTHAFLLSQTLEIHKRMPPLHMHKSPAEVGAEGNGVSGGSQGGPDSKGGSERGSPIEKYMRPSKQASYSPPGSPIEKYQYPIFSLPFIHSDLQSEADWLRFWTKYKMSVPGNPGHYLGHVASLPNPCQSFVPYPTFGLPPHFPPPPPSSAVPDSDTPLDLAMKHSKPSPMPNGANMVKEKAQLEKRASPLADRESEWDRPIEAKEEDAVPPQPTKPDRVDQSTQDDLSSKCAHCGIVFLDEVMYALHMSCHGDGGPFQCSICLHACADKYDFTTHIQRGLHRPAPDASAKATDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D701</accession>
    <name>A0A3B4D701_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017562338.1">
        <property type="nucleotide sequence ID" value="XM_017706849.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D701"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018901"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028380.2">
        <property type="protein sequence ID" value="ENSPNAP00000018901.1"/>
        <property type="gene ID" value="ENSPNAG00000025450.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432759"/>
    <dbReference type="CTD" id="116447"/>
    <dbReference type="GeneTree" id="ENSGT00940000167650"/>
    <dbReference type="OMA" id="KFKDNFW"/>
    <dbReference type="OrthoDB" id="47179at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    <sequence length="867" mass="100296" checksum="2CC2CD0861F59545" modified="2018-12-05" version="1">MKVKKEEKDEKGKRKSAHHQEKHEHRHRKKSNSPSEETVKERHSKLNGKHHRSSANHCGSGSLGNVLQESRISSSGKVKADSANSASDADSMDVDTKEGIEKSLMSLDGNNNLAAMTPWHEESGNGEVKKETPEETSKHKRKSKHNNDGHSHKKEKKRDKGDELQTSREKNNRSSKTSKSSTGDKRHKTSAKVKRSENSPMSETAIKEEKDELPNFDLISGCGQVKSDAEMTKEKHSPGKAAEKEPTARTTKEKKRGQEKKRKSNSLEHDEAKKNKVKKEEDEYEDKKEESEKKAKQAEEEADKWKWWEEGKHAENGQKWRSLEHKGPLFPPEYEPLPSSVSFLYNGTPVILRPATEEVATFYAKMLDHEFTTKEVFQNNFFTDWKEVMTETERQLISDLSQCDFSNIHKYFVEKTEEKRNMTKEEKQVLKEEANKLTEEYGYCLLDGHREKIGNFRVEPPGLFRGRGEHPKMGKLKKRVQPEDVIINCSKDVKIPEPPPGHRWKKVQHDNTVTWLASWVENIHGHIKYIMLNPSSKLKGEKDWQKYETARKLKLSVDSIRSEYQSAWTSCEMKQRQYGVALYFIDKLALRAGNEKDDDTADTVGCCSLRVEHINLHRHLDSLDYVVEFDFLGKDSIRYYNKVPVEKQVYKNLKLFMNSKNPDDDLFDRISTTYLNKKLNEAMPGLTAKVFRTFNASTTLQEQLNQLTTDDMTLPEKILAYNRANRAVAILCNHQRAAPKTFEKSMQLLQEKIKKKQEEVEKAKKELKQAKKEHKQLPSEVSKKLVQKMEKTVKRLLEQLKKLQLLETDRNENKVIALGTSKLNYLDPRISVAWCKKNNVPVEKIYNKTQRAKFAWAIDMADENFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8M9</accession>
    <name>A0A3B4D8M9_PYGNA</name>
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        <recommendedName>
            <fullName evidence="7">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="primary">CBX4</name>
    </gene>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8M9"/>
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        <property type="gene ID" value="ENSPNAG00000027611.2"/>
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        <property type="component" value="Chromosome 30"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:SUMO ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Chromobox protein homolog 7"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CBX4"/>
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        <property type="entry name" value="CBX7_C"/>
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        <property type="entry name" value="Chromo-like_dom_sf"/>
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        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
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        <property type="entry name" value="Chromo_dom_subgr"/>
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        <property type="entry name" value="Chromodomain_CS"/>
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        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE CBX4"/>
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        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE CBX4"/>
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    </dbReference>
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        <property type="entry name" value="CBX7_C"/>
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        <property type="entry name" value="CHROMODOMAIN"/>
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    <dbReference type="SUPFAM" id="SSF54160">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="7">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
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        </location>
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        <location>
            <begin position="450"/>
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    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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            <end position="461"/>
        </location>
    </feature>
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        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020712.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="513" mass="57967" checksum="C134F12EA4CC56C2" modified="2018-12-05" version="1">MELPAAGEHVFAVESIEKKRIRKGRFEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNRERQEQLMGYRKRGPKPKQLLTQVSSFARRSSVLSGLQEASQDNSLKAVVDPAQTQQYQLNSRKLHQYQPICKESSGDALVNGKKKHYYQLNSKKHHHYQPDPKMYQDVKGTEVDKESWNLPKALPQKWLQDKDVVCLNKVKDITIGLEKLPANLHGGVKSELAKEDVNQANGVSNRLKIVKNKNKNGRIVIVMSKYMENGTQAAKIKNGEMDVGARQQELLENQSDVTDKNKHSKKHSLENGWKKDNKDDGTPPPCGFTNGISGPAVDGLVTSSTNKAKELHSSSPDQSSQLTTKSCMNSKLLQKAVLHHLDQKTSQGTHKRRYSEPNSERDSEAKRFLSFRSISAPNAETSHCQSNGHQNSNGHRFEFSDCQDEPMDLSCSRSRKENSSITNCQINGNSKTSEKEVAPQQERTQKDPESEHAPSFTPFLGNIIITDVTANCLTVTFKEYVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBP0</accession>
    <name>A0A3B4DBP0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor HES-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Hairy and enhancer of split 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017560377.1">
        <property type="nucleotide sequence ID" value="XM_017704888.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBP0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020890"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031768.2">
        <property type="protein sequence ID" value="ENSPNAP00000020890.1"/>
        <property type="gene ID" value="ENSPNAG00000027846.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431617"/>
    <dbReference type="GeneTree" id="ENSGT00940000163346"/>
    <dbReference type="OMA" id="QANTILW"/>
    <dbReference type="OrthoDB" id="6085656at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097150">
        <property type="term" value="P:neuronal stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11461">
        <property type="entry name" value="bHLH-O_HES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000033">
        <property type="entry name" value="Transcription factor HES-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="15">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="16">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="163" mass="18840" checksum="75F0B4C2E21E546C" modified="2018-12-05" version="1">MTPAVASAMTISKEHLPLNNKLRKPVVEKMRRDRINSSIEQLKTLLTPEFLNQQPDSKLEKADILEMTVSFLRRQQQKQPAISTSSTAVSQGFSRCVHEVVHFLSKDEMKTQSQRKLLNHFQNLQPSTEEKWRESDLPLLSSTDQQAIRKEKSSVQSALWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEJ0</accession>
    <name>A0A3B4DEJ0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEJ0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021878"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040849.2">
        <property type="protein sequence ID" value="ENSPNAP00000021878.2"/>
        <property type="gene ID" value="ENSPNAG00000029122.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443321"/>
    <dbReference type="GeneTree" id="ENSGT01150000286911"/>
    <dbReference type="OMA" id="NFRNEPP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000100">
        <property type="entry name" value="Si:dkey-11n14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="110"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="787"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021878.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="984" mass="112609" checksum="FED12EC5E49EC3D2" modified="2024-11-27" version="2">MEHKQPDEDILQKVLRNHKASMKSKYECLFEGIPTHHNRTLLNNIYTQLYIIEGESEGVNEEHEVLQVQNMFQGCNKGIPVNYSDIFSPLPEQNLEEEHERNGLEERKFKTVLTKGIAGIGKTVSVQKFVLDWAEGKANQDVDFMFMIPFRELNLIKDDQYSLHGLLCVFHPELKGLDPKGYDVCKIMFILDGLDESRISLNITASSQSKTISDVTKNSSVDVLMTNIIKGELLPSALIWITSRPAAASQIPSRYVSRVTEIQGFNDPQKEEYFKKRISDPDQASRVFSQIKKAKTLYIMCHIPIFCWISSIVIQQILEQDTCGEIPTTLTEMYIHFMLIQTKTKNLKYHVKDDERDLKKREKEMILKIAELAFKQLIKGNILFYVEDLRECGIDVTEASVYSGVCTEIFKEESVLYQKKVFCFVHLSFQEFFAAVFGFHACVEREAEVVKMFQSRSKSNGGFVDQFMMSAIDKALTSRTGHLDLFLRFLYGISRESNWKLLYHIVPPKCRISEDFEDTIQRVRQKHPKNVSPERWINFLHCFIEMKDNSFQREVQTLLNAKRKLTGWDCTRIAYSLQMSEEVLDEFDPKKYKTSDEGRRRLIPVVRNCRKALLANFKLTKQHCEIVVSALQSANSVLTELDLSYNDIQDSGVKQLCECLKNPHCKLETLRLVMCNLTKKTCEYVASALQSPNSGLKELDLSQNDLEDSGVRLLSGGLKSSDCQLEILRLSGCLVSEEGCASLASALSSNPSHLRELDLSYNHPRESGMKLLGAKVDDPSYKLDTVNFNYGGVYTMKAGVRKYACELSVDLNTVNTELVLSENNRKVTYVWEEEKQQSYPDHPERFQCPQLLCRESLSGRCYWEAEWSGRRAGIAVAYKGTTRKFECGSVEFGSNEHSWGLFGYNYKKKYSVNHKHKKTFIPAPRSCSNRVGVYLDWPAGMLSFYSVLSDGSTLSHLHTVQCRFTEPLYAGIFVYDASLSLCHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJ66</accession>
    <name>A0A3B4DJ66_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SKICH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CALCOCO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548263.1">
        <property type="nucleotide sequence ID" value="XM_017692774.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017548264.1">
        <property type="nucleotide sequence ID" value="XM_017692775.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJ66"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023171"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013310.2">
        <property type="protein sequence ID" value="ENSPNAP00000023171.1"/>
        <property type="gene ID" value="ENSPNAG00000007712.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424629"/>
    <dbReference type="CTD" id="386968"/>
    <dbReference type="GeneTree" id="ENSGT00950000183025"/>
    <dbReference type="OMA" id="CALFHAC"/>
    <dbReference type="OrthoDB" id="10015001at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012852">
        <property type="entry name" value="CALCOCO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041611">
        <property type="entry name" value="SKICH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051002">
        <property type="entry name" value="UBA_autophagy_assoc_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31915:SF5">
        <property type="entry name" value="CALCIUM-BINDING AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31915">
        <property type="entry name" value="SKICH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07888">
        <property type="entry name" value="CALCOCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17751">
        <property type="entry name" value="SKICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKICH" evidence="10">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium binding and coiled-coil" evidence="9">
        <location>
            <begin position="295"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="500"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="144"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="369"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="518"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="65785" checksum="566618BE357A3D00" modified="2018-12-05" version="1">MEKPWRVKFRNVDQTYFPQTRVECRYTISGHHKWSSRDWIGLFKAGWASMKDYYTFAWALAPEGYTVGTEVNCSVLFQPSYLPSPGGTAFQFVYIDESGEICAVSPQFTFCAPRPLDELVTLEEERNGAEENEGEDLLLVVPKAQILQSHLDACQQELKQLQKKLDAATEEAKKQRERNETSKMDFEKERAEMKSEIEELRDSLRCSVEKIQQMEEKQKDMLKSSESISAEVSVLLAEGTENQQRIKELEEDLLNLTQQKQETDAELDRMKERVKKMTTQRKDEEDERRNLQTESVQAHEELRVLQERLEASERSTEALRRDLSELGALQSHSHAELHQVRLQAAQMTLQLSQANLALREGQAAWSQERESMRQSAELDKERIQKLSRELQKKEEWLQEERTEREKLELELGNEKDCNRELRASLRAIQKEREQQQLEKQELLDHIHLLQLRMEREADAKWTEAASATLNIESAPSAIETFGHNDLEEPPEIQPLIHKERDEDNQEQTEQESSIGTREQSKSETDRKEMVPVNLGVQDDPNANKDKALILSDIKGHIPSDLADFPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJT2</accession>
    <name>A0A3B4DJT2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dapper homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DACT1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dapper family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555333.1">
        <property type="nucleotide sequence ID" value="XM_017699844.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJT2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023740"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011930.2">
        <property type="protein sequence ID" value="ENSPNAP00000023740.1"/>
        <property type="gene ID" value="ENSPNAG00000008247.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428673"/>
    <dbReference type="CTD" id="51339"/>
    <dbReference type="GeneTree" id="ENSGT00950000183181"/>
    <dbReference type="OMA" id="HKRGDYR"/>
    <dbReference type="OrthoDB" id="9448112at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046329">
        <property type="term" value="P:negative regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000095">
        <property type="term" value="P:regulation of Wnt signaling pathway, planar cell polarity pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024843">
        <property type="entry name" value="Dapper"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15919:SF12">
        <property type="entry name" value="DAPPER HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15919">
        <property type="entry name" value="DAPPER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15268">
        <property type="entry name" value="Dapper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="279"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="378"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="556"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="748"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="295"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="595"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="752"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="90093" checksum="5462333FAE8CCD3B" modified="2018-12-05" version="1">MKTPLAEMLAALREPRERAERERAEGREEWERARTRERLDAALAGLGELEYLRHRQEGLVRGALTQREERGSASASGEEESYLNKEEKLLEENILLLRKQLNCLRRRDAGLISQLQELDRQISDLRLDTETSHDHVETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCRSSTCLCAPLDTSLCASDGRSKYADELGVCAECEGQCEDSSSGTVRRSLSAPYSPSPDGPCDSLSKYHCDLIAKNGNDVYRYPSPLHAVAVQSPIFFQSVASHLKEEGGLSKSGENPCENQKPEQPQNTSWPASQTPLNKRLDSYIFGLLQRRAQPVRTNKPRTSINTDPSKSVLRQASLCVRPPTAGQTQPRTSELKVNWQTSLQAAATSSTDPSTVSPQRQWSVESKGEHVENGTSYSQSQPPNGYLINGDINTNSLLKKRGSGANKGQPLTVVAKECQELGSPNAVSSPKLNKHSHYPAVEDCKSAQKAGTPKRNQKIQTPVAGSVKVEWPAPELISLGSSSQSQDEGGQMVSAQYIPAQEQNVKLRKGGTKNVKIVKVKNSTSTKSRTHAADPCSEAGRDRHHRAGSRRFRPVEDASHSHKASKRTSCKAKRIPVAIPEGQILEKHSSSTSRSSSQRHHGHHRHHEAVLAKPKYKRNDYHRRLRGIPEMPYEEAFRRAHRRQKRENLGHISAMYLPSSAHFTSPYAYVGSDSEYSAECASLFHSTILDTSEDERSNYTTNCFGDSESSASEADFVAESSTSSDSEGSAGVNWPQCSQASAGSTLTREMTSAQAKAFVKIKASHNLKKKILRFRSGSLKLMTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLW7</accession>
    <name>A0A3B4DLW7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">COL8A2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017549753.1">
        <property type="nucleotide sequence ID" value="XM_017694264.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017549763.1">
        <property type="nucleotide sequence ID" value="XM_017694274.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLW7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024448"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010077.2">
        <property type="protein sequence ID" value="ENSPNAP00000024448.1"/>
        <property type="gene ID" value="ENSPNAG00000008990.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425550"/>
    <dbReference type="CTD" id="1296"/>
    <dbReference type="GeneTree" id="ENSGT00940000154317"/>
    <dbReference type="OMA" id="KHGMPGQ"/>
    <dbReference type="OrthoDB" id="10021193at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF53">
        <property type="entry name" value="COLLAGEN TYPE VIII ALPHA 2 CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044005752" evidence="7">
        <location>
            <begin position="21"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="484"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="689" mass="67285" checksum="DA708E92DF533766" modified="2018-12-05" version="1">MTMLLVPVCVLLMLGGHALGGGYAPMPQMKYMQPMMKGPVGPPFREGKGQYLDYQPSMKVKGEPGPQGKPGPRGPPGPAGLPGKPGIGKPGLNGQPGPQGPSGLQGIGKPGLPGLPGKVGPKGLPGNNGEVGPQGEPGPRGLPGQPGSPGPAGLSLNGKPGLPGVRGPPGSRGDPGPKGGRGLPGDKGLKGENGNGKPGQPGIRGPSGLQGPPGPPGNPGVGKTGLNGLPGPAGPKGDQGLPGLQGLPGDAGPPGQLGPPGPTGLGKPGVDGVPGRPGPLGPKGEPGVRGPPGFPGAPGYGKPGLIGQKGDRGLSGVPGAPGDKGEPGLDGLPGDMGPNGQAGPPGQQGPMGLPGKHGMPGLKGELGPQGPPGLPGLRGDQGPSGLPGKPGIPGDKGLPGPNGAIGKPGPKGEGGHIGLPGNPGLTGGPGSKGENGFPGPPGPRGLSGIPGLPGPTGHMGPQGIPGLKGEQGLPGPPGNGKNGDQGLPGPQGPPGKTGLPGINGIQGPPGPPGPPGPPGPSNGDTTVAGLQGPDIAGESITRPGVEKPQFGVGEFSATMAPAFTAILTTPFPPSGMPIKFDRTLYNGQNAYNPATGIFTSPLPGIYYFAYHVHVKGTSLWVALYKNNVPATYTYDEYKKGYMDQASGSAVLELKENDQVWVQMPSDQANGLYSTEYIHSSFSGFLLCPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DP23</accession>
    <name>A0A3B4DP23_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 26 member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SLC26A1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>bromide(in) + chloride(out) = bromide(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75335"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>iodide(in) + chloride(out) = iodide(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72379"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>nitrate(in) + chloride(out) = nitrate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75339"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxalate(in) + sulfate(out) = oxalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:72275"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>oxalate(out) + 2 chloride(in) = oxalate(in) + 2 chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:75095"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sulfate(out) + 2 chloride(in) = sulfate(in) + 2 chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:75091"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569251.1">
        <property type="nucleotide sequence ID" value="XM_017713762.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017569261.1">
        <property type="nucleotide sequence ID" value="XM_017713772.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DP23"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003720"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008233.2">
        <property type="protein sequence ID" value="ENSPNAP00000003720.1"/>
        <property type="gene ID" value="ENSPNAG00000010144.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436973"/>
    <dbReference type="CTD" id="10861"/>
    <dbReference type="GeneTree" id="ENSGT01150000286920"/>
    <dbReference type="OMA" id="WNENQDL"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000015">
        <property type="entry name" value="Sulfate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="435"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="486"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="19">
        <location>
            <begin position="542"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="710" mass="78546" checksum="F89437A49D6B3714" modified="2018-12-05" version="1">MDTTPLEDMEVAKSPPMISLERKVRPRKGPVEIIRTKLRRQLSCSVPKVKSTITDFFPVMLWLPKYKIKEYAWGDLMSGLIVGIILVPQAIAYCLLAGLEPIYGLYTSFFGNIIYFILGTSRHVSVGIFSLMSLMVGQVVDREVYLAGFDLSDESKQSSLDSEWNITEDFSKTAINLTINAFNIKCGKECYAISIATALTFLAGVYQVVMAVFRMGFVSIYLSAPMLEGFATGASCTILTVQAKYLLGLKIPRHQGYGTVVVTWINIFKNIHNTNFCDMITSAICIAVLLGGKELQERYKNRLKMPLPTELVVVALATIVSHFADLNGHYSSSVSGAIPTGFIPPQVPDFELMPRLALDAIPLAVISFAFTVSLSEMFAKKNGYTVRPNQEMLAIGFCNIIPSFFHCFTTSAALAKTMVKDSTGCQTQVSSVISAFVVLLVLLFLAPFFYSLQKCVLACIIIVSLRGALQKFRDFPRLWRLSKIDAVVWMVTMSSAALISVEIGLLVGVVFSMICVVAQTQYPKVSLLGQIENTNHYEDMGDYNSLLSIPKVKIFRFQAPLYYANKDFFLKSLYKAVGLEPFLEITRRRKIEKKAKIRAAKKPDEENGDVSIGLITSELDFHTIVLDCSSISFIDTTGTNTLKGLLKEYKEVGVNVILACCNTKVIDSLRQASFFGSDDKEMYTVSFHTVHNAVSFAIDDAAASVNASSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQ51</accession>
    <name>A0A3B4DQ51_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574424.1">
        <property type="nucleotide sequence ID" value="XM_017718935.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQ51"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025580"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007255.2">
        <property type="protein sequence ID" value="ENSPNAP00000025580.1"/>
        <property type="gene ID" value="ENSPNAG00000010744.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440175"/>
    <dbReference type="CTD" id="558970"/>
    <dbReference type="GeneTree" id="ENSGT00940000156282"/>
    <dbReference type="OMA" id="CRCANCA"/>
    <dbReference type="OrthoDB" id="6162476at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF123">
        <property type="entry name" value="DOUBLESEX-AND MAB-3-RELATED TRANSCRIPTION FACTOR 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="8">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="6">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="372" mass="41818" checksum="F8E93EE976769892" modified="2018-12-05" version="1">MSAGVEPELTRLEGEDEEVDVETVEAKRRPSRSPKCARCRNHGVVSRLKGHKRLCRWRDCRCANCALVVERQRVMAAQVALRRQQAAVGKKSQRSASFLRRSAYQCYSRAPSLLAKSILEGYKPPVLEDWPKRLHYPPLSVRMRKRRAFADKELESVMLEREMRQREMEELPELVFLQATVSPAPSPCCCSLSDSGLPSHLPMYKSSPLLFECDLHCHTREFKLSSVEQCQRGDILTQSCLLQTAKSWETCNERDSCLESFLLPSFQSSQYYCSENSGVNTEQTVPENVTADSSSSVPNGVAADRLDIASKQDGALSLIEAKVLSRAELPNKHSRAPGLENLTQKHCDSKTNSVKNSSTRPLPFSVEALLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DSR3</accession>
    <name>A0A3B4DSR3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">creatine kinase</fullName>
            <ecNumber evidence="2">2.7.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017539254.2">
        <property type="nucleotide sequence ID" value="XM_017683765.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DSR3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026004"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006196.2">
        <property type="protein sequence ID" value="ENSPNAP00000026004.2"/>
        <property type="gene ID" value="ENSPNAG00000001648.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411951"/>
    <dbReference type="GeneTree" id="ENSGT00950000182772"/>
    <dbReference type="OMA" id="IEDFCFP"/>
    <dbReference type="OrthoDB" id="432281at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000014">
        <property type="entry name" value="arginine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF52">
        <property type="entry name" value="CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="9">
        <location>
            <begin position="39"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="10">
        <location>
            <begin position="156"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="159"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="318"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="346"/>
            <end position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026004.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="401" mass="45512" checksum="D01CDB4657562C71" modified="2024-11-27" version="2">MWKRNKVWLIKPPAEHPLEAKYRPLLESLVPPDPMSKLKLKRGSPREEFPSLDGNYTCMARVLDLHMYTRQFNRATESGVIFDDIIRPGLEDPGTRTGPMTVGCLAGDAQSYILFCDFFDRVIEAYHSYKVSCDVMQESDFNYDNLKGGDAFDPAYTESCEVSVARCVEDYCFPTHCSRGERRQLLTLAKKVLDEFSEEFPGKLYSIDDLNQETKDKGIIMDGPPASLIKTGVGRDWPDARALWVSDDGTLAVWVNMEDHLRLVSSRNDANIQEAFATICIGLLKMEALYKKLRHPFIWKPHLGWVVSSPAEVGTGLKASVTIKLVHLVTHKRLDNILDRLRLCIEKACLHGMYKICNRQTIGLTEVELTQLVVDGVNLLIRMEKRLENNEGFDDLVPAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DSY0</accession>
    <name>A0A3B4DSY0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037400055.1">
        <property type="nucleotide sequence ID" value="XM_037544158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DSY0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005671.2">
        <property type="protein sequence ID" value="ENSPNAP00000026211.2"/>
        <property type="gene ID" value="ENSPNAG00000001793.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431941"/>
    <dbReference type="CTD" id="557754"/>
    <dbReference type="GeneTree" id="ENSGT00390000018542"/>
    <dbReference type="OMA" id="RAMSFIQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035196">
        <property type="term" value="P:miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039691">
        <property type="entry name" value="ZC3H7A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14928">
        <property type="entry name" value="MICRO-RNA BINDING ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14928:SF6">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="803"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="803"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="354"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="681"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="864"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="698"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026211.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="899" mass="99848" checksum="5C2CD8B1FD46B161" modified="2024-11-27" version="2">MDPERQKRKEEIDKALGFIQSSLPFPDSEGYEAFLTQLVCNLLDEGNTVFRDGEWRQAAAHYSEGVNVARYAQSEALVIPPELLESLYVNRAAAHYNLGEYERGVQDCDSALCVSEGSRRALYRKALCLRELGRFREAYECGTACLLTAPHDRQVSELAQDLAVKLGLKLRKAYISTQDDTLASQTDSNGETASSTGDTSNGLESLTDIGSDLANAQCIPAPLATPIPVSDDPPMPSLSPVVPQDTPESPNQVPTGRVQYSVPVSEHMGECVINEDLDSLLENIPKGAEVSVNPVQGAIPTNLPNTAVGLRPPYPSGLPASSSQLTPPYFASVVSPLPQLDAFSVLGQSESSTQPMESLISFSSETGDSPGRGRAESRSTEGLDSLSEYTLPGGRISTNFIPGLRNLSAIHTQNGSTTTNLSLLSRNPLAATHEFRQACHACYSRIGPRVMDYKYQPEAAHRCKRDVLLCRLKSSDDTTWKRIRPRPARNNFLGAFVLCKEVQERQECQYGENCTFAYCQEEIDVWTQERKGALSRELLFDPLGTNERRALSVTRLLQLHMGMFMFLCEECFDSKPRIISKRSKENLGVCSNLTARHPFDDNKCLVHVVRSANVRYSKIRPLHPLCQFDVCRHEVRYGCQREDSCSFAHSVIELKCWVLQQDTGITHEEMVQESKRHWQRLEQNAQRQKPMHVPHQTSSSSNAVSSAGVGSGGMAGGGSDSIGGGGSGGGSCGRARGLNLKMKFVCGQCWREGQVNEPDKALKYCTAKAKHSWTKERRVLLVKSFEKKKWVVVRPLPFSRTYPQQYDMCVHVMKQKKCHYIGNCSFAHSLEERDVWTYMKNNSLRDMQQMYDLWLSLTNQNRRSDGTLITPPPEEKQSTLPADYTESMGGQRLSGNGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DT93</accession>
    <name>A0A3B4DT93_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oxidative stress-induced growth inhibitor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Monooxygenase catalytic activity. Involved in regulation of cytokinesis; promotes RHOA activity, probably acting locally at the midbody in late cytokinesis. Monooxygenase activity is involved in stabilizing transient structures between daughter cells, termed intercellular bridges, before abscission. Regulates differentiation and proliferation through the regulation of cell death.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the OKL38 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563931.1">
        <property type="nucleotide sequence ID" value="XM_017708442.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DT93"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026321"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005420.2">
        <property type="protein sequence ID" value="ENSPNAP00000026321.1"/>
        <property type="gene ID" value="ENSPNAG00000011608.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433705"/>
    <dbReference type="CTD" id="393155"/>
    <dbReference type="GeneTree" id="ENSGT00390000006658"/>
    <dbReference type="OMA" id="MDLHDKE"/>
    <dbReference type="OrthoDB" id="412005at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000152">
        <property type="entry name" value="Oxidative stress-induced growth inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029731">
        <property type="entry name" value="OSGIN1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15192:SF15">
        <property type="entry name" value="OXIDATIVE STRESS-INDUCED GROWTH INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15192">
        <property type="entry name" value="PROTEIN CBG05349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="340"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="519" mass="57224" checksum="C82F3C888C057FFB" modified="2018-12-05" version="1">MDPRNKEPLCEDVLPVVIIGNGPSGICLSYLLSGYTPYLSPEGFHPNPVLHSKLTQNPHLSLFEQDLEYLCEGVEGRSSNPVAVLFDSLLLPDSDFGLDYASPLEWRYEPERATPHLVLGKGPPGGAWHAMEGSMLTLSLANWMELPGLKLKDWMREKRRNVRNDRATPAEIASYYQHYVRQMGLERSFACGTTVTSVRRGSLRSGRSVWNVQGLQRRAAEGEQQTDAEIPFSVWAENVVLATGTHDIPARLGVEGETLPFVCHSFWELEAAISSGHLDQASEPILVVGAGLTAADAILAAHHLNTPVYHAFRRSVADPALIFNQLPKLLYPEYHKVHQMMSQQQRQPGESPADMVSQRLSSPRDERASSPCSYPGYLSFPKHRVVAFRPDGKCVLEGEGGRQTVLQVSMALVLIGAHPNLSFLPEQGRPLSLEPHEPISCRRNPVDVDPYTYESVKEAGLYAMGPLVGENFVRFLKGGALAIVCDLARKQTMRGQKNVQESSAGHSLSCQLSNCTLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUT1</accession>
    <name>A0A3B4DUT1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUT1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026841"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004098.2">
        <property type="protein sequence ID" value="ENSPNAP00000026841.2"/>
        <property type="gene ID" value="ENSPNAG00000002136.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162556"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001756">
        <property type="entry name" value="Si:dkey-185m8.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF107">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="20"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026841.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="274" mass="31412" checksum="FDD96F6E9780E436" modified="2024-11-27" version="2">MNKQKQREDFRLKMCETNHLSELRIVLLGCLEAGKSSAGNTILGREEFDSQITAQCVKRQGEVAGRRITVVEAPGWWINSLVEESTEFRKQEIVLSVSQCPPGPHAVLLVIPLGIQFTENDRTALQKHLKLLTESVWSHTIVLFTCGDSLGDTPIEKHVESEGRALQWLVEKCRNRYHVLNNENRSDDTQVKELLEKIEEMVAANSGRQFELDREILEEVEEKRKVEEDRAKERTTKVQKRRKDIQAKMGLQAFYFIYTAFVSTDVCVCVCVCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DY42</accession>
    <name>A0A3B4DY42_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Organic solute carrier partner 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">OSCP1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DY42"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029327"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016349.2">
        <property type="protein sequence ID" value="ENSPNAP00000029327.1"/>
        <property type="gene ID" value="ENSPNAG00000015372.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433591"/>
    <dbReference type="CTD" id="100334608"/>
    <dbReference type="GeneTree" id="ENSGT00390000004808"/>
    <dbReference type="OMA" id="GTMFNKR"/>
    <dbReference type="OrthoDB" id="2157380at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019332">
        <property type="entry name" value="OSCP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439">
        <property type="entry name" value="OXIDORED-NITRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439:SF0">
        <property type="entry name" value="PROTEIN OSCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10188">
        <property type="entry name" value="Oscp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="44502" checksum="B275DE0CE1F94B98" modified="2018-12-05" version="1">MSTRTLPLLFINLGGEMLYILDQRLRAQNIPSDKAKKATVAGWIEEDRRRVMNDIITTMFNKKFLEELFKPQELYSKKALRTVFDRLAHASIMRLNQASMDKLYDLMTMAFKYQVLLCPRPKDILLVTFNHMDAIKDFVRDTPSILSQVDETYRQLIEIYTPLSGGEFQLIRQTLLIFFQDMHIRVSIFLKDKVQNSNGRFVLPTSGPVPYGTHTPGLIRMFSCNGEEVKRTQFRDGGNYTSALREGSFELFGDRVIKLGTNMYSVSRPVETHMSGTSKNSSQHTKVNTIPNPLAKEELNLLAKLMGGLEVQKPSNTDSGFRVNLFATDEEEEEALISRPEELSYEVINIQATKDKQANAELAKIMGEFTEAGEPSPSSSSKGDDLLAMMDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZS9</accession>
    <name>A0A3B4DZS9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actin interacting protein 3-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SRCIN1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZS9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035819.2">
        <property type="protein sequence ID" value="ENSPNAP00000029912.2"/>
        <property type="gene ID" value="ENSPNAG00000016306.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157961"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061001">
        <property type="term" value="P:regulation of dendritic spine morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF5">
        <property type="entry name" value="SRC KINASE SIGNALING INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3-like C-terminal" evidence="4">
        <location>
            <begin position="183"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="284"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="574"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="808"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="853"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="954"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1214"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1327"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="620"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="699"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="577"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="588"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="821"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="955"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="996"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1021"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1053"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1089"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1107"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1231"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1276"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1349"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1384"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1410"/>
            <end position="1422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029912.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1430" mass="157616" checksum="D096FB3476D587D5" modified="2024-11-27" version="2">VGNRRGGGHIMSTDDLEYPREYRTLGNGRRFSNVGLVHTSEHRHTVIAAQSLETLSNLHKADMERKRDAFMEHLKNKYTPQHSLPPSPSPSHSSMRGSSERSAREQVQTYSYFASRSPRHSQSTQSGLADQASKLSYASAESLETMSEADMPLGFNRMNRFRQSLPLSRSASQSKLRSPGVLFLQFGDETRRVHITHELSSLETLHALIVRMFPQKLTASMLKSPNTAILIKDEARNVFYELEDVRDIQDRSIIKIYRKEPVYSSYATAAHLANGDLRREMVYTSRESSPTRRLNTLPSSTSSPSGSSPSRSRLSYGGGGRPPSFGGPGHPHPSGGSQHGHHPSPSHGPGAGGLTASPSAILERRDVKPDEEVSGKNMVLLKNEGLYADPYNLVHEGRLSIASTQSLATIADPFSFPVSGGLYRRGSVRSLSTYSAAALQGDLEDQLYKPGGSIYSDAYGPGSSTLTMGFRLPPSSPQKVPDVPLRDRDSYSGPPSRGSPVRQSFRKDSASSSVFVESPKSRPGSSSEPLGDVGRSASGFGSPLAGNEMDTRDRMEAMEKQIASLTGLVQSVLTRAPDSDSTEKTESTSDGSATGTLTPSAPLALMPPPAMTPQGSNVSRLQMQLHLHELKQNATDLRKQLDQLRKMQLQNQESVRSLMKRTEMELSVRVSDALRKQEDPLQRQRLLVEEERLKYLNEEELIIQQLHDLEKSVEEIQKESSVNHRHITVQELEEKATVLRRLGETLTELKNQFPGLQSKMRVVLRVEVEAVKFLKEEPHRLDALLKRCKTITDTLATLKRQVNEGIWKNQDDFSSPSSKHMGGEDFRKGSDFDIPTSPPLNFNESSLANWSPHVHAHTHGHSHSNPSSGQRERDSHGVGSLKGRVLEELGGRGGSDKASSVEVRLAAERDWEEKRASLTQYSAQDINRLLEETQAELMKAIPDLDFAAKQIKPVQNQNQSQNPNQNQNQNQNEAPTSAHSVPEHKPSKAQHAHKVSSKEGGSRRGSDELTVARYRTEKPSKSPPPPPPRRSFPSSPGLTTRSGEVIIPGKSLKKSESDETEVQKPHVKLRRSVSEAPRPASTPPTVSGGEREDREEEKVAAELEVETTSSANSSDVQRGPSSSTSVPQIVLTECISHPTSPTECSERGSFSDMEKLSNMPQRDKNTGSGIALSYFSHIVKIPSLSLWKQDLAGSQRKCSEMQVLDGDQKEFQARLKSSPAMPGTPSASPEQKAEKQPRKIELRIQESFEKEDQTDMCERDPRPGLLPQRRAMPEPSETSKAVASVTRTQGVGQEAGKEDIKHSTYRRLDSLEETIRELELSLMEFSTHPNMGHYPTLPQPHSPSAEYPTASSRLQRSSTGAVSSEPRVKSPGESLRPPVPPKPSISTDLTKSGSGKAISRLKHLQQGGSEKSKLGKQREDYIKSQGQQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZU1</accession>
    <name>A0A3B4DZU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZU1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028524"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014756.2">
        <property type="protein sequence ID" value="ENSPNAP00000028524.2"/>
        <property type="gene ID" value="ENSPNAG00000003059.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234513"/>
    <dbReference type="OMA" id="MPQLSKC"/>
    <dbReference type="OrthoDB" id="9441654at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF118">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="11">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="12">
        <location>
            <begin position="139"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028524.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="255" mass="29389" checksum="8F8AD329126BA424" modified="2024-11-27" version="2">MNWTGLQNLLSGVNKYSTAFGRVWLSMVFVFRVLVFVVAAQRVWGDDSRDFVCNTRQPGCTNVCYDSIFPISHIRLWALQLIFVTCPSLLVVAHVKYREEKDKKYAEAHKGSHLYANPGKKRGGLWWTYLLSLVFKAGFDTAFLYILYYIYDGYDMPQLSKCSLKPCPNTVDCFISRPTEKRIFTLFMVITSAICIFMCVCEMFYLIGKRICKLHKKEHRFAAFNDFCITLEKVVIWCVKSSASPPALSYTTSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3K1</accession>
    <name>A0A3B4E3K1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LMO1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3K1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029814"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017287.2">
        <property type="protein sequence ID" value="ENSPNAP00000029814.2"/>
        <property type="gene ID" value="ENSPNAG00000016162.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153908"/>
    <dbReference type="OMA" id="IRDRYML"/>
    <dbReference type="OrthoDB" id="6352355at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09388">
        <property type="entry name" value="LIM1_LMO1_LMO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09389">
        <property type="entry name" value="LIM2_LMO1_LMO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000015">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000016">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF2">
        <property type="entry name" value="RHOMBOTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="91"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029814.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="161" mass="18478" checksum="98DC188CAC7E4149" modified="2024-11-27" version="2">LLIKKKEKVQNMKSVPMLSVQPKGKQKGCAGCNRKIKDRYLLKALDKYWHEDCLKCACCDCRLGEVGSTLYTKANLILCRRDYLRLFGTTGNCAACSKLIPAFEMVMRARDNVYHLDCFACQLCNQRFCVGDKFFLKNNMILCQMDYEEGQLNGSFETQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E543</accession>
    <name>A0A3B4E543_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:ch211-13f8.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017570138.1">
        <property type="nucleotide sequence ID" value="XM_017714649.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570139.1">
        <property type="nucleotide sequence ID" value="XM_017714650.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E543"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030436"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018484.2">
        <property type="protein sequence ID" value="ENSPNAP00000030436.2"/>
        <property type="gene ID" value="ENSPNAG00000017176.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437511"/>
    <dbReference type="GeneTree" id="ENSGT00390000013003"/>
    <dbReference type="OMA" id="QRGRPGM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060341">
        <property type="term" value="P:regulation of cellular localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032756">
        <property type="entry name" value="DUF4685"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051741">
        <property type="entry name" value="PAR6_homolog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14102:SF15">
        <property type="entry name" value="KIAA1614 ORTHOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14102">
        <property type="entry name" value="PAR-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15737">
        <property type="entry name" value="DUF4685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="1004"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="85"/>
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            <begin position="276"/>
            <end position="314"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="666"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="884"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
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        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="898"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1092" mass="119047" checksum="E0082DB543FD5110" modified="2024-11-27" version="2">MLMEGETPGAPDSTALHISVERTADTQELLSALQSESKSPSPSSGSPSSSPLLSGSAVSALQTKFKQISQRRCKEKDKHEYLFEGSSEHCVQNIDEDKLSPSQTPSSDLRKTSSGSDEDGSEPHLTEVCDKGDKESTTFGIAGLPRGLGEGSSLENIATGGADTSLSPKPTSSPRHWAPPKGFWRVARPETLLLNGEGACSLVPCSPKNVPHTQEEELQRKRRLRVECAAVRKELQRSDSLESTFHRCFQKEADPTDTLGDLWRADSWETVCSRGGSKMERAGTDENHSEKCKTRTMETTTGKRAEGQHQPPDLIYRGLPPSYKEADWDTAITHGAELTVRSVLAPGDLTLSPRHEQAKRLLERARSKARSQNPDGDHATRPADREDLELLTLHESPPPHRTIIVATQEQLVPPPVQLLEVRGRNSERGIPLRRHGQSPTRVRFEDESEKEAERRYLDRVRERGPVVAERSQSNQQPKAEPILTISQVSKGSGTSWGSVGIPVAILTDEGEGTSLETVVLKEDLRKCEACGSILAPVHAKESSSKSLLSSAEGKSTPRWVTPGHSGRQIIRPALLGGVMLTEGSNLVENVEVAAVGGAGERMSAFGKLRRRSRKGESRVETSYGPYARGQELLAQRRNSRTRSSLEEITGFHSKHTETQTRGVTFAIDTLPGADSSQSTTTTSRNARDSPGPQLPIKSALKSTSKIRPTGQRVVKLLPSVQYRLIHLDKEMEGGSHHQDVAPEEHLSVTLGTSSQASQATSGLTPCIRPSSLRYSSPVLTPELQALAIWDTVDPVDAHLSSDDVCRDSTCASPHPPECRPAVRGMGMSKAEDLRAELLRAEHRKAELQWDENLAGSRRGAEREGKPKLSLRRFFSAMGLNSMGKLVKGNRSSSMEQLSSPAPKHSSASPSPTHRGQPPLQRTPSLQSLHTESPLAQLRKVSSVQSLQSPKRKFERSTILGELPLPLSLGPRELQVEGGMEQLEGSRSVGPLGRLVQAFPDGTLLLELKRPANGHFGFVISRGKGRPDSGIYVEQVGGSPTDNVYTGLLGVGDEILEVNGEKVTGLNLDHVTRLMTRESTATIRILPHRWIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8P7</accession>
    <name>A0A3B4E8P7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tryptophan 2,3-dioxygenase</fullName>
            <shortName evidence="10">TDO</shortName>
            <ecNumber evidence="10">1.13.11.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Tryptamin 2,3-dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tryptophan oxygenase</fullName>
            <shortName evidence="10">TO</shortName>
            <shortName evidence="10">TRPO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tryptophan pyrrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tryptophanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TDO2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-tryptophan + O2 = N-formyl-L-kynurenine</text>
            <dbReference type="Rhea" id="RHEA:24536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="1.13.11.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="10">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Homotetramer. Dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the tryptophan 2,3-dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.11" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8P7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032957"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023352.2">
        <property type="protein sequence ID" value="ENSPNAP00000032957.1"/>
        <property type="gene ID" value="ENSPNAG00000021285.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424996"/>
    <dbReference type="CTD" id="100008541"/>
    <dbReference type="GeneTree" id="ENSGT00390000008593"/>
    <dbReference type="OMA" id="FITIHQT"/>
    <dbReference type="OrthoDB" id="447477at2759"/>
    <dbReference type="UniPathway" id="UPA00333">
        <property type="reaction ID" value="UER00453"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:L-tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019442">
        <property type="term" value="P:L-tryptophan catabolic process to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3810:FF:000001">
        <property type="entry name" value="Tryptophan 2,3-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01972">
        <property type="entry name" value="T23O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004981">
        <property type="entry name" value="Trp_2_3_dOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138:SF2">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03301">
        <property type="entry name" value="Trp_dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1 10" id="KW-0349">Heme</keyword>
    <keyword evidence="5 10" id="KW-0408">Iron</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0823">Tryptophan catabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="410" mass="48273" checksum="7684E99C8088DEEC" modified="2018-12-05" version="1">MNALAMSGCPYFQRKHLLASKQQVKEDDVDAEQSEVNKPSKGGIIYGDYLQLDKVVSSQLLQSELKGNKIHDEHLFIVTHQAYELWFKQILWELDSVREIFISGHVRDERNMLKVNTRIHRIVMIFRLLVDQFAVLETMTALDFFDFREYLTPASGFQSLQFRLLENKIGVPESQRVPYNRRHYRDNFRGQDIELLQQSEKEPTLLQLVEKWLERTPGLEEDGFNFWGKLESNIFEGLRRGKDKIEQKPDSEMKEELMADFIKQKELFMCLFDEKRHNHLLSKGERRLSYKALQGALMIYFYREEPRFQVPFQLLTSLMDIDTLMTKWRYNHVCMVHRMIGSKAGTGGSSGYQYLRSTVSDRYKVFVDLFNLATFLVPRDWVPKLNPNEHTFLYTAECCDSSYCSSEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EA85</accession>
    <name>A0A3B4EA85_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017562522.1">
        <property type="nucleotide sequence ID" value="XM_017707033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EA85"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032613"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022684.2">
        <property type="protein sequence ID" value="ENSPNAP00000032613.1"/>
        <property type="gene ID" value="ENSPNAG00000020695.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432902"/>
    <dbReference type="CTD" id="100536173"/>
    <dbReference type="GeneTree" id="ENSGT00950000183079"/>
    <dbReference type="OMA" id="AYMSSYE"/>
    <dbReference type="OrthoDB" id="5859791at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16175">
        <property type="entry name" value="EFh_MICU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF10">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="419"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="479" mass="55150" checksum="B854A21C4DF8B3B7" modified="2018-12-05" version="1">MMAALRRLISAGHKCIAYTSGAGLHPKSTRSLRRAGAGLGLGLGGIAVYELWLSRDDEKQGCRISQHCGLVRLPGVAAKEKTKIRADDEDMAMSAHEHRFRLFSSVEYEGQLYMTPLDFIESVTMSEPKKKKFWRSLTKKDLDKILSDTPPVWKGSSRFFRNLHEKGIISYTEYLFLLCILTKPHAGFRIAFNMFDADGNEMVDKREFMVLEEIFRKKNDKKERGGDSDSSSQLSVELYGCQGSVSNAVLKKEQPQFEPRSFWDVLKQGARIVLFSDLVEQVQENITDTTLLLHFFGKKGKTELNFEDFYRFMDNLQTELLEIEFLAYSKGMTTISEEDFARILLRYTTVDNISSYLENVRQSIPDEKGITFEEFRSFFQFLNNLEDFAIAMQMYNFASRSIGQDEFARAVYVATGLKLSRHLVNIVFKIFDVDHDDQLSYKEFIGIMKDRLHRGSWGYRGEERFTSFKACMKKELSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EB63</accession>
    <name>A0A3B4EB63_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="21">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="21"/>
    <dbReference type="RefSeq" id="XP_017546717.1">
        <property type="nucleotide sequence ID" value="XM_017691228.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EB63"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032938"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023355.2">
        <property type="protein sequence ID" value="ENSPNAP00000032938.1"/>
        <property type="gene ID" value="ENSPNAG00000021242.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423715"/>
    <dbReference type="CTD" id="556237"/>
    <dbReference type="GeneTree" id="ENSGT00940000155422"/>
    <dbReference type="OMA" id="PIWPQAE"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000010">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF55">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 21" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="89"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="532"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="621"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="461"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="633" mass="71760" checksum="01CC64E06FD9549C" modified="2018-12-05" version="1">MLPPSRRDFVSLTVSESGGYTNGKQRRQSCWRKWKQLSRLQRSLILFVLVLLLIGGIATYPSLSAQWGDTAVEENSVEGKQPFVVELKNQGRPLLPEPPSEQRRNSHKRGPPVLQSRLQNKGNSSELRGAEVKNVEEGDGSKPLISWRGAVIEAEQGTDPNAKEKEKAVEPEDPSAAQLVESRLEAVREAFRHAWKGYKDFAWGHDELKPVSKSYSEWFGLGLTLIDALDTMWILDLKKEFEEAKQWVATELSFNKNVDVNLFESTIRILGGLLSTYHLTGDALFLEKAKDIGSRLMPAFSTPSKIPYSDVNIGKGTAHPPRWTSDSTVAEVTSIQLEFRELGRLTGDPKYQAAVMEVMQQVHKLEGKHDGLVPMFINTNNGQFTHLGIYTLGARADSYYEYLLKQWIQGGKKETELLEDYLQAVEGIKKNLLKKSSPLGLTFVGELSHGHFTPKMDHLVCFLPGTLALGAHHGLATEHMELAQQLMETCYQMYTQMETGLAPEIAHFSMQTGSIQDVDVKLADRHNLLRPETVESLFYLYRFTKDKKYQNWGWEILQSFNTYTRVETGGYTSINNVRDPKYPSPRDKMESFFLGETLKYFYLLFSDDPNLISLDKYVFNTEAHPLPIWPQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EC66</accession>
    <name>A0A3B4EC66_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EC66"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033293"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024036.2">
        <property type="protein sequence ID" value="ENSPNAP00000033293.2"/>
        <property type="gene ID" value="ENSPNAG00000021841.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286927"/>
    <dbReference type="OMA" id="SRHCELE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="104"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033293.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="722" mass="81908" checksum="4301A8C693434D06" modified="2024-11-27" version="2">MSDINMFIVIVYCNKLFSELAPACHQKFKSSLKKKFHRINERIPYHGGSTLLNEIYTELYITEGGSGEVNIEHEVRQIETASRRPATQERPIKCNDLFKDTAVRTVLTNGVAGIGKTVSVQKFILDWAEGKANQDILFIFPLPFRELNSVKSRKLSLVDLLRLFFTDIKQLKPADYYQYKVLFIFDGLDESRLPLDFQNNESLSDVTQSASVDVLLTNLIKGNLLPSALLWITSRPAACGQIHPECVDQVTEVRGFSDPQKEEYFRKRISDQSVANRVITHIKTSRSLYIMCHIPAFCWIAATVLERMLGEAESGEIPKTLTQMFTQFLIFQIKHKDQKYHGKCDTDPRQTREMILALGKLAFQQLEKGNLIFYEEDLRECGIHVREVSVYSGVCTQIFREELGLRLGKVFSFVHLSVQEFLATLFVFFSFISQNRNVLEHQTHSKSTVTDFLKSAVDRALQSENGHLDLFLRFLLGLSLESNQTLLRGLLTQTGSPSNCREETVEYIKEKLRENLSPEKYINLFHCLNELNDHSLVQEVQTYLNRGNYHRLDEVPLSPAHWSALVFVLLTSEEELDVFDLRKYAPSEECFLRLLPVVTASRKAVLSQCRLSERSCAALASVLSSNSSSLRELDLSYNRLCDSGVTLLSAGLEDQHCKLETLSLSRCRLSERSCAALASVLSSNSSSLRELDLSGNSLCDSGVTLLSAGLENQHCKLETLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECU0</accession>
    <name>A0A3B4ECU0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573339.1">
        <property type="nucleotide sequence ID" value="XM_017717850.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017573340.1">
        <property type="nucleotide sequence ID" value="XM_017717851.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017573341.1">
        <property type="nucleotide sequence ID" value="XM_017717852.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECU0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033655"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024631.2">
        <property type="protein sequence ID" value="ENSPNAP00000033655.1"/>
        <property type="gene ID" value="ENSPNAG00000022343.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439433"/>
    <dbReference type="GeneTree" id="ENSGT00940000165445"/>
    <dbReference type="OMA" id="EGNMVSY"/>
    <dbReference type="OrthoDB" id="2015213at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052896">
        <property type="entry name" value="GGT-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881:SF1">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="562" mass="60853" checksum="9FC76D9672EBEBEB" modified="2018-12-05" version="1">MKMKDRLVFSSHRSPVICLNGCVASSQPLASNIGLDILKRGGNAADAAVAVAAALSVTEPMSTGLGGDAFCLFYDAVTRQVRGLNGSGRSPTAQTLELMERHGFSQSNPPPTFHALNVTVPGAAACWCDTVALFGSKKLSLADLLQPAIGLAQRGFPVAKIAAYHWARGTETLRAAGRELGADFLINNQPPKHGQIMTNPSLACTLQELALQGKAGFYEGRIAEAVVDVVKENGGVLSLDDMKNHVTVEITPIYADYKMVRLWEVPPNSQGMAALIALNILENFPIKDMGHMSPDYLHVLVEALKLSMTDTMHFSSDPDQVKVPIEGLLSKDYAQQRAQSIQMNKANSVCERGIPTGSDTVYFTVVDREGNACSFVNSNYMGFGTGLVPRNCGFSLQNRGANFSLDPDHVNCVGPKKRPYHTIIPAMLTEASSGRLLCSYGVMGGFMQPQGHVQVLLNMLEFGMNPQVALDAPRVFVHLDQAAQKWHLNLEDGVEQRVAEDLRGRGHVVNWPIEGHERAQFGRGQVISVGTWWDPTNEKPEGSERVLWAGSDPRADGCALGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EE68</accession>
    <name>A0A3B4EE68_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NIF3-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NIF3L1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May function as a transcriptional corepressor through its interaction with COPS2, negatively regulating the expression of genes involved in neuronal differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Interacts with COPS2. Interacts with THOC7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550615.1">
        <property type="nucleotide sequence ID" value="XM_017695126.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EE68"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034822"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027035.2">
        <property type="protein sequence ID" value="ENSPNAP00000034822.2"/>
        <property type="gene ID" value="ENSPNAG00000024332.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425990"/>
    <dbReference type="CTD" id="60491"/>
    <dbReference type="GeneTree" id="ENSGT00390000003590"/>
    <dbReference type="OMA" id="KYHEFFD"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000004">
        <property type="entry name" value="NIF3-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017222">
        <property type="entry name" value="DUF34/NIF3_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037490">
        <property type="entry name" value="UCP037490_NIF3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034822.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="429" mass="46371" checksum="5941701BBCFAF0C9" modified="2024-11-27" version="2">MFSECRIVSGSLLRLSCRLSPLNLSCSATFKRPSCPCSSLHHSASNTFGNRPVLFLSHLSSLLSSPHRSQKASRWLSQAAVSMELKAVLEVLEQLAPLSLAESWDNVGLLVEPSKPHPVKAILLTNDLTSAVMDEAEAMQCDLIISYHPPLFRPFKRLVQKDWKQRLAVRAIEGGIAVFSPHTSWDSANGGLNDWLIGGLGGGRVSVLSQALCSAPQKNRLEFTARGQEELNNILKLLKETENSEAFQYTVLRNEEGGQQVILTCPSSALTPVVQTVLGNTTASQSLSITQVQQPPMLGCGQGRHIVLDEPVSVAVAVQRMKTHLGLPHLRLALGEQRSLDAVVKTAAVCAGSGASVLQGVKADLYVTGEMSHHEVLDAVAKGTSVVLSDHSNSERGFLSVFKERLASRLPDSVTIAISKRDRDPLEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFM4</accession>
    <name>A0A3B4EFM4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Presequence translocase associated motor 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PAM16</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572514.1">
        <property type="nucleotide sequence ID" value="XM_017717025.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFM4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034094"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025600.2">
        <property type="protein sequence ID" value="ENSPNAP00000034094.1"/>
        <property type="gene ID" value="ENSPNAG00000023152.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438904"/>
    <dbReference type="CTD" id="51025"/>
    <dbReference type="GeneTree" id="ENSGT00390000012037"/>
    <dbReference type="OMA" id="AKYLIQI"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13796" checksum="D62D4AC243D0CEF5" modified="2018-12-05" version="1">MAKYLAQIVVVGVQVVGRAFARALRQEFAASQAAAEARGRAGKQSAAASSFTGMTVQEAQQILNVSTLKPEEIQTNYEHLFKVNDKAAGGSFYLQSKVVRAKERLDEELQIQKQDPLEQKQQQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK32</accession>
    <name>A0A3B4EK32_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Interleukin 23, alpha subunit p19</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK32"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030514.2">
        <property type="protein sequence ID" value="ENSPNAP00000036250.2"/>
        <property type="gene ID" value="ENSPNAG00000026947.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235094"/>
    <dbReference type="OMA" id="LTAVNWY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043523253" evidence="1">
        <location>
            <begin position="24"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036250.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="190" mass="21553" checksum="12514A0C80A8DBD5" modified="2024-11-27" version="2">MGSFRCLAIVAALLALVFECAHGAPAGARTKRHLDYSAGRTLATQLNEKARYLYREVEIPNELAVVSDPPLMIRPEDKCDPDSLQSNAEPCLSKVILALKNYSRIFGDNGLFREEYTKCSKWRASATDVFNITTQLLRALKEQVEAQEPLKLEKWMSEVFLCRDSVDRLYSFSILTARIFNFLSHHHNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELP1</accession>
    <name>A0A3B4ELP1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SH3BP2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555719.1">
        <property type="nucleotide sequence ID" value="XM_017700230.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELP1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030326.2">
        <property type="protein sequence ID" value="ENSPNAP00000036189.1"/>
        <property type="gene ID" value="ENSPNAG00000026805.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428876"/>
    <dbReference type="CTD" id="6452"/>
    <dbReference type="GeneTree" id="ENSGT00390000002216"/>
    <dbReference type="OrthoDB" id="10254483at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13308">
        <property type="entry name" value="PH_3BP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000043">
        <property type="entry name" value="SH3 domain binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000147">
        <property type="entry name" value="SH3 domain-binding protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035848">
        <property type="entry name" value="SH3BP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15126:SF4">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15126">
        <property type="entry name" value="SH3-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="57"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="570" mass="63296" checksum="F90AF91771AF73EE" modified="2018-12-05" version="1">MDLLPALSSAASMFSESMAVKTIRRRMSKRNMATAQISWPVPMRAIGAQNLLTMPGGVSMSGYLHKKGGSQFSLMKWPLRYIIIHKGCIYYFKTSTSAAPQGAFSLNGYNRVMRAAEETTSSNVFPFKIVHFSKRHRTWYFSAASEDERRKWMRHLRKEIDHYNDKKDYLDASESESDAESFYGPIESPLEVTSVPDHHEDDEISQDEDSDDEDYLKPDGSPGRSTVPPPSYPPPPVPHQDRDVCTPNSHRKGPPPPLPPPPNKTPPCPYPKKFPVIVPGPSMHLDSINKGPPPLPPNPSHSPKQFLNRATGGSLNPSLPAPPPAILKKSISTLPACDKKPASSVVIKVPSTLPICHDLEKKVVLNTGEKPRLLSMHNLSQGSKSAEERKPNSSTTTANKSPLMIPKPPPPGIKPKSIRPSLQRSSPDGQSFRSSVEENPLKRRVKLQNGNEDSDDDDYENVQLPDSVFVDTTETTMVEKLFKEAASSPQDGLYCIRNSGTKTSKVLVVWDVSLNKARNYRVFEEDSTVFLEADMTFPSLAALVEHYYSHPLPNHGSLCLQQPYGYTPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EPZ4</accession>
    <name>A0A3B4EPZ4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017552509.1">
        <property type="nucleotide sequence ID" value="XM_017697020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EPZ4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037256"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033148.2">
        <property type="protein sequence ID" value="ENSPNAP00000037256.1"/>
        <property type="gene ID" value="ENSPNAG00000028903.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427081"/>
    <dbReference type="CTD" id="558571"/>
    <dbReference type="GeneTree" id="ENSGT00390000010968"/>
    <dbReference type="OMA" id="WHPVEVT"/>
    <dbReference type="OrthoDB" id="1708389at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034164">
        <property type="term" value="P:negative regulation of toll-like receptor 9 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13221">
        <property type="entry name" value="PH-GRAM_GRAMDC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037847">
        <property type="entry name" value="GRAMDC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037845">
        <property type="entry name" value="GRAMDC4_PH-GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37402">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37402:SF1">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM" evidence="4">
        <location>
            <begin position="475"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="607" mass="69440" checksum="325BCE3701DA3BD4" modified="2018-12-05" version="1">MKLCSDSRAGVTIHHVKVKPKLGVLNMLKKLDKIRFRGPKRDEFLDLAESPTASDNEGSEDILVKPRPSLRDPEELRDPAGSGSLNMATMQDLRSDSERLNEVKGHLEIALLEKHFLQEELRKLREETNVDTLKQELEKERGRRLDLEQKMSEVLKSRLEDSPPQPSRKQQSPSANGAEKQQKETLCSRLQKWLYDRFGVYIEDFHFQPEENTVEPEEPLSAKRLTENMRRLKRCARPITNFLRNLSALSNWHSVYTSAIAFIVYMNAAWHGWAIPMFLFLAILRLSLNYLIARGWRIQWSIVPEVSEPLEPPKEDLTVSEKFQLVLDVAQKAQNLFGKMADVLEKIKNLFMWVQPEITQKLYIGLWVAFISSCVLPYKLMGLIIGLYAGIKFFIIDFLFKSCPKLRDKYDTPYIVWNNLPTDPQLKERSNATVSRRLSGCEKIQPVVSRSSLATIPSGVSREDDSGRAHNTKKGAFHEIFNLPETERPLPVCENGWRCCLINRDRKMPTDYIRNGVLYVTENYLCFESSSSRSGSSKKNKVIKLVDITDVQKYKVLSVLPGSGMGISIATPSTQKPLVFGAMIHRDEAFEAIFTQYMKIVTTGPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ER46</accession>
    <name>A0A3B4ER46_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A</fullName>
            <ecNumber evidence="2 13">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TRMT61A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>adenosine(58) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.220"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(1)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55392"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12415"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2 13"/>
    <dbReference type="RefSeq" id="XP_017580477.1">
        <property type="nucleotide sequence ID" value="XM_017724988.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ER46"/>
    <dbReference type="SMR" id="A0A3B4ER46"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037626"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034208.2">
        <property type="protein sequence ID" value="ENSPNAP00000037626.1"/>
        <property type="gene ID" value="ENSPNAG00000029500.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444029"/>
    <dbReference type="CTD" id="115708"/>
    <dbReference type="GeneTree" id="ENSGT00940000154239"/>
    <dbReference type="OMA" id="RPDHRMI"/>
    <dbReference type="OrthoDB" id="1925287at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.20:FF:000002">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000097">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="15">
        <location>
            <begin position="64"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="114"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR017269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="304" mass="33222" checksum="31A4E5D230B1DA42" modified="2018-12-05" version="1">MSFVEYSEFIQEGDVVIIFLGHDSMMPIKVEAGAQTQTRYGAIRHSSDLIGRRFGSKVTCSKGGWVYVLHPTPELWTVNLPHRTQILYTTDIANITLMLDLKPGSIVCESGTGSGSLSHAILRTIAPSGHLHTVEFHAQRAEKAAQEFKEHKVAHLVTVRNQDVCKEGFGVVGVADAVFLDIPSPWEAVTHAKKAFKRKGGRLCSFSPCIEQVQRTCEALLEQGFEEICTVEVLQRVHDVRSVTLHLPDFGPEDGGAESAKMASEEVLLAANSSHPSVTCRTATAPREMAGHTGYLTFATKPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKG2</accession>
    <name>A0AAR2IKG2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037392341.1">
        <property type="nucleotide sequence ID" value="XM_037536444.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392346.1">
        <property type="nucleotide sequence ID" value="XM_037536449.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392350.1">
        <property type="nucleotide sequence ID" value="XM_037536453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKG2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050892.1">
        <property type="protein sequence ID" value="ENSPNAP00000038221.1"/>
        <property type="gene ID" value="ENSPNAG00000034176.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108437332"/>
    <dbReference type="GeneTree" id="ENSGT00390000014139"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036128">
        <property type="term" value="C:CatSper complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060296">
        <property type="term" value="P:regulation of cilium beat frequency involved in ciliary motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028746">
        <property type="entry name" value="CatSper1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053873">
        <property type="entry name" value="CATSPERG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003915">
        <property type="entry name" value="PKD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47193">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47193:SF1">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22846">
        <property type="entry name" value="CATSPERG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01433">
        <property type="entry name" value="POLYCYSTIN2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043759032" description="Ion transport domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="470"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="CATSPERG C-terminal" evidence="8">
        <location>
            <begin position="56"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="300"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="642" mass="73987" checksum="8DD835EE935E28B5" modified="2024-11-27" version="1">MKATLLVLLLSAVAGMLTCDHQHSEQEAGPGCPLGKRLAFDRNTTLEESKRLNNEYFDCPNPEPGAPCFYYGHVFYPFFVIEDTVVGESDRFHDSYTFKVVGGGRTRESVRNYSQEEILKFNSLRDSESYQQTLVWTFDDVPDSFPLTKEGFIVMRGASNRVRWLCQRNSPCGDLTPVNHLDPEFYFVVEVSNMETAGSPFCEYTVRFELHVHGFPLDPVRALFFHQMTMTFVQTILICYIGHCWRLKRKTVYTKTRDGLDEQEGHEQFNAGDVGVKVGCTLRSCFLQLYHIIYSIVQNPVLDHVILLVVLLDTSTLMAHTFQSVTVTTSWCLSAVNSCLLVFYVLEALFKILIQGRTYFRNPWNDLDFFIVLMSLLDFVLSLVQSGGGFSSRQASTLFRVLKTLKGARVFRALRLLKAIRFFKGLQAILLTCLQSFRSMSSILVLMFLFLFIFAVVFREMFNESDPEHFGSIFRTIFTLFQILTLDDWSLIYMTSRDNGAPHIIYFMSLYILVELFTFLNLFIAVLVDNFQLSICKKRHHKSQKSPDVKEGEAQSTKKAVEDLDVSRTEIEEEFYKEALRHASSDGKHSKRKIELTKRYLQLLAAMDQHMQKYRSQACLLDNLVETFFMDQDEETRSDAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKI9</accession>
    <name>A0AAR2IKI9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TSPAN33</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKI9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000088201.1">
        <property type="protein sequence ID" value="ENSPNAP00000038246.1"/>
        <property type="gene ID" value="ENSPNAG00000008502.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159484"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046931">
        <property type="term" value="P:pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03158">
        <property type="entry name" value="penumbra_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000007">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF154">
        <property type="entry name" value="TETRASPANIN-33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="156"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="283" mass="31977" checksum="E816A8BFF0B46037" modified="2024-11-27" version="1">LTRRPASESARGDEEFSFVSPVVKYMLFCFNMIFWMISLLLISIGVYARVTKHETALACLTVDPSILLMIVGVLMFFITFCGCVGSLRENICLLQTFCICLTIIFLLQLAAGILGFVFSDKVRHKVTEMINNAIVHYRDDLDLQNLIDYGQKEFACCGGLSYMDWSKNIYFNCTDDNPSRERCSVPFSCCLISKDERVINTMCGHMMQDLDHNKASAFINTNGCIDKLVNWIHSNLFLLGGIALGLAIPQLVGILLSQVLINQIQDQIKLQDYNVQHRSDPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IL70</accession>
    <name>A0AAR2IL70_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone H3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IL70"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055716.1">
        <property type="protein sequence ID" value="ENSPNAP00000040460.1"/>
        <property type="gene ID" value="ENSPNAG00000033184.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287006"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000047">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="4">
        <location>
            <begin position="44"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="99" mass="11187" checksum="327AF64B23C82883" modified="2024-11-27" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAAGGLKHGFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IS16</accession>
    <name>A0AAR2IS16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GNAT1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="GPROTEINAI"/>
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        <location>
            <begin position="40"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
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        <location>
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        <ligand>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>A0AAR2ISF5</accession>
    <name>A0AAR2ISF5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myc target 1b</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017547967.1">
        <property type="nucleotide sequence ID" value="XM_017692478.2"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSPNAT00000046964.1">
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        <property type="gene ID" value="ENSPNAG00000030878.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000011642"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Myc_target_1"/>
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    </dbReference>
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        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
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        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
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        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
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            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23043" checksum="C381EA3E78B7DE3B" modified="2024-11-27" version="1">MADNSTHPILEILQSFGLRDLILAFCLSMVVGLVLGALVYVTLTWMSRRRASATITRLPSSRCVGRSSSSSSPRSRAAFGRHNSAHDRRSNNSLASAAFSFHRQASCSSPTSDQADLLGRKSSFRASTFHPLLQCSQIAREAEEGSQGSLPRTPTLNNNPTATGSTTTVSSMVTPPRARPRPDSFWGNNGLRGVQTPPPAYESIIRAYQETCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IUU2</accession>
    <name>A0AAR2IUU2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DAPK3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_037391245.1">
        <property type="nucleotide sequence ID" value="XM_037535348.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391247.1">
        <property type="nucleotide sequence ID" value="XM_037535350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IUU2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044153.1">
        <property type="protein sequence ID" value="ENSPNAP00000043498.1"/>
        <property type="gene ID" value="ENSPNAG00000010482.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444344"/>
    <dbReference type="GeneTree" id="ENSGT00940000153424"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14105">
        <property type="entry name" value="STKc_DAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000110">
        <property type="entry name" value="Death-associated kinase 3, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000250">
        <property type="entry name" value="Death-associated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042870">
        <property type="entry name" value="DAPK3_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF18">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="13"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="378"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="453" mass="52343" checksum="C094B5BAE02CFB82" modified="2024-11-27" version="1">MAGFRQEDVEKFYEMGEELGSGQFAIVRKCREKSTGVEYAAKFIKKRRLSSSRRGVSREEIEREVNILKEIQHSNIITLHDIFENKTDVILILELVSGGELFDFLAEKESLTEEEATQFLKQILDGVHYLHSKHIAHFDLKPENIMLLDKNVPNPRIKLIDFGIAHQIKAGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSNTSELAKDFIRRLLVKDPKKRMTIEDSLEHPWIKVIKRRNVRQEDSGRRPERRRLKTTRLKEYTIKSHSSMPPNNTYVNFERFSHVLEEIGAAEEGLRRLQQSQRSCRDDVAALRSIYEEKEGWYREESESVAAELGRVRSELQRAQAQRRQSQEEARAATLAANALKRRFGRLESRYESLAQQVAAEVRWVEELLRSSSTEAEERITGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IXB8</accession>
    <name>A0AAR2IXB8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Growth arrest-specific 2a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GAS2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578349.1">
        <property type="nucleotide sequence ID" value="XM_017722860.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IXB8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074163.1">
        <property type="protein sequence ID" value="ENSPNAP00000042697.1"/>
        <property type="gene ID" value="ENSPNAG00000035504.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108442691"/>
    <dbReference type="CTD" id="557312"/>
    <dbReference type="GeneTree" id="ENSGT00940000155755"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.920.20:FF:000003">
        <property type="entry name" value="Growth arrest-specific 2 like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.20">
        <property type="entry name" value="Gas2-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003108">
        <property type="entry name" value="GAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036534">
        <property type="entry name" value="GAR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46756:SF9">
        <property type="entry name" value="GROWTH ARREST-SPECIFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46756">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02187">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00243">
        <property type="entry name" value="GAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143575">
        <property type="entry name" value="GAS2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51460">
        <property type="entry name" value="GAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="64"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="GAR" evidence="7">
        <location>
            <begin position="235"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="38908" checksum="A351A719D1A3036D" modified="2024-11-27" version="1">MHIESRRDALSRAEVSQSARRCGRSVRSSMCSSLSPKQQPAGPGLTDLQQYSQWLASRHEASLLPMKEDLALWLTSMLGVEITAESFMDRLDNGFLLCQLAETLQEKFRQSNGELLPPGKPIPKRRIPCRASAPSGSFFARDNTANFLSWCREVGVGETCLFESEGLVLHKQPREVCLCLLELGRIASRYKVEPPGLIKLEKEIEQEEKAPEPPPSPASPTQVSSPNKCSSCKKSTGKLLDDAVKHIAEEPPCKCPNKFCVERQSQGRYRVGEKILFIRMLHNKHVMVRVGGGWETFESYLLKHDPCRMLQISRVEGKTSPISSKSPSIKDLTPDSYLVVAAHYKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYS7</accession>
    <name>A0AAR2IYS7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PCDHAC2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYS7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063202.1">
        <property type="protein sequence ID" value="ENSPNAP00000043199.1"/>
        <property type="gene ID" value="ENSPNAG00000031371.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000288661"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="410" mass="43629" checksum="4663AB9C8549C580" modified="2024-11-27" version="1">KEKNQERNKGKRQMDREALLERPTTRRKAVLNAGRLSAPVLNAGRLSAPVLDAVRLFAPVLDAVRLSAPVLDAVRLSAPVLDAGRLSAPVLDAVRLSVPVLDAGRLSAPVLDAVRLSVPVLDAVRLSAPVLDAVRLSLPVLDAVRLSAPVLDAVRLDAVRLSVPVLDAVRLSAPVLDAVRLCVPVLDAVRLSAPVLDAVRLSAPVLDAVRLSAPVLDAVRLSLPVLDAVRLDAPVLDAVRLDAVRLSVPVLDAVRLSAPVLDAVRLCVPVLDAVRLSAPVLDAVRLSAPVLDAVRLSAPVLDAGRLSVPVLNAGRLSVPEWSIQSVMVYLFTSHAHGHSGRQPTQRKQQKKVSSGGVTTRRAVLLITAGRQNSCVRLVTKYPSTSHTGQLDFSGTVLMNTSNQGHRQRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J848</accession>
    <name>A0AAR2J848_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ZNF511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J848"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043593.1">
        <property type="protein sequence ID" value="ENSPNAP00000046487.1"/>
        <property type="gene ID" value="ENSPNAG00000014006.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011381"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039258">
        <property type="entry name" value="ZNF511"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21354">
        <property type="entry name" value="ZINC FINGER PROTEIN 511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21354:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="323" mass="36705" checksum="157D234F7AB83E34" modified="2024-11-27" version="1">MLQSSLLQLLNGGDAARVYIHTIPVLEKSEEIQSVSGGGGAEENPSPFRFTPHTIRLDRDDEFFEDGDVHRHLYLQDLVTALADETQAPNVSEFRCHIAGCKQLFDTLEGYEHHYNSLHRHVCSNCKRSFPSNHLLDVHILEWHDSLFQVMAEKQCMYQCLVEGCGLKFQTSKERKNHLITVHSYPSDFRFDRTKKSKRTKEKKTLKQWEVCMAVSGHAVNQHGEPENCEAMDVSLPQQSEKQELPPEQQKPRYSYKVPSSICFGQGSVRGFRGGRKKNACLTGGSGVSGLREAPRSPGKSVRPARRVRRRGRFQLYGSLLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J9X3</accession>
    <name>A0AAR2J9X3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tropomodulin 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMOD2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J9X3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074096.1">
        <property type="protein sequence ID" value="ENSPNAP00000048735.1"/>
        <property type="gene ID" value="ENSPNAG00000022863.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158280"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000006">
        <property type="entry name" value="Tropomodulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF15">
        <property type="entry name" value="TROPOMODULIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="42783" checksum="4A702B2B076B9D8F" modified="2024-11-27" version="1">MALSFKKDLEKYKEIDEDEILNQLSEEELKQLESALEDMDPENALLPAGLRQKDQTSKTPTGPFNRDGLLQYLEKEAMEYKDREDFVPFTGEKKGKVWVPKQQPIETRKEEVTTLDPELEEALSSATDTELCDLAAILGVHTLVTSTQSYDGTTNTDGYNNVIKGEKVKPVFDEPPNPTNVEETLQRIKANDPSLTEVNLNNIKNIPIPTLKDFAKALEKNAHVKKFSLAATRSNDPIAVAFGDMLRENKTLRSLNLESNFITSAGVQALVEALRDNDTLSEIKIDNQRQQLGTTVEMEIAKMLEANTSIVKFGYHFTQQGPRARAAAAITKNNDLGNTHTATDLANVNFMMNGPKEMTQNHLVKILVPLTSIKSKVCFFLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JCM9</accession>
    <name>A0AAR2JCM9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Folate receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folate receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576195.1">
        <property type="nucleotide sequence ID" value="XM_017720706.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JCM9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071141.1">
        <property type="protein sequence ID" value="ENSPNAP00000049798.1"/>
        <property type="gene ID" value="ENSPNAG00000036737.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108441274"/>
    <dbReference type="CTD" id="83546"/>
    <dbReference type="GeneTree" id="ENSGT00950000183144"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902444">
        <property type="term" value="F:riboflavin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004269">
        <property type="entry name" value="Folate_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018143">
        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10517">
        <property type="entry name" value="FOLATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10517:SF25">
        <property type="entry name" value="RETBINDIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03024">
        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043647296" description="Folate receptor-like domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Folate receptor-like" evidence="6">
        <location>
            <begin position="27"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="236"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="271" mass="29835" checksum="FBE1888FA2C227D2" modified="2024-11-27" version="1">MTVARLLLGLAYVATFFTTFVWCQEGACLQDGRHKATPSPEPYLKECSMYTENACCLEQDIDDLTSPPAGADSAPWDRCGTLSPVCEAFLKRVVCFYRCSPHAAHWPHPQRDSFLKAVPLCHSFCRDWYEACKTDLTCARDWAGDPRGQNCTGSCVPYQQMYQHGRDLCESLWGDAFMTVEDEAGEEDRVEGHGCGCLTLSPSDRDVIAALRAQKDNPDELDTTKSGLPQYRAPCAHAAAAPPQTRSSNANSVLRKRSVPIHDTEGSGSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDT3</accession>
    <name>A0AAR2JDT3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Suppressor of cytokine signaling 5b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546087.1">
        <property type="nucleotide sequence ID" value="XM_017690598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JDT3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001499.2">
        <property type="protein sequence ID" value="ENSPNAP00000048001.1"/>
        <property type="gene ID" value="ENSPNAG00000002738.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108422458"/>
    <dbReference type="CTD" id="559639"/>
    <dbReference type="GeneTree" id="ENSGT00940000164285"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000028">
        <property type="entry name" value="Suppressor of cytokine signaling 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022252">
        <property type="entry name" value="SOCS4/SOCS5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF15">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12610">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="403"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="493"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="105"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="62092" checksum="2414B4EACC104124" modified="2024-11-27" version="1">MEKAGKMWSNLRNRCHTLFHASGSETNVCSLDMDRFHCVVDLSQGPHTGESQAARGSASSQVPVVRGGRRGHNCVADIPQIVEISVDKENEDVRRVPLVRRDSYSRHAPWGGKKKHSCSTKTQSSLEADRRTGRSRGGVSRRERCYGTSSIQEVSDSGGGGSGRSLSVRSVRQRLKDTVGLCLPLPTPRRPRSAKSQVASKRKIHLTELMLETCPFPPGSDLANKWHFIKQHTAPVSPHSSTALLDAIDPVRASPEDEEERLRERRRLSIEEGVDPPPNAQIHTLEASAQRSALYKLGPKMAPGSSEGAGEVRSFGAGTSFSGGFSGIVAQVGASMAMPSQAADCDSEEDSTTLCLQARRTKQRHASGDGHSSRQSGPWKVHTQIDFIHCLVPDILAITVLPCYWGVMDRYQAEALLDGRPEGTFLLRDSAQEDYLFSVSFRRYNRSLHARIEQWNHNFSFDAHDPCVFHSSTVTGLLEHYKDPSACMFFEPLLTAPLHRTFPFSLQHLARAAICHQTTYDGIGALPLPPAMQDFLKEYHYKQKVRVRWLERELPLKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDY2</accession>
    <name>A0AAR2JDY2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JDY2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046039.1">
        <property type="protein sequence ID" value="ENSPNAP00000048429.1"/>
        <property type="gene ID" value="ENSPNAG00000036840.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167495"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000016">
        <property type="entry name" value="Protocadherin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF276">
        <property type="entry name" value="PROTOCADHERIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="10">
        <location>
            <begin position="11"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="10">
        <location>
            <begin position="70"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="263" mass="28903" checksum="DDE802A513F7C6AE" modified="2024-11-27" version="1">MAEYKNQTIVMDEATGVLSVSESLDREQQGTYVLTVLAVDGGSPPRSSTASVTIFVEDINDNQPQVILPSSNLSCLTVSPSTRTGSIVTKIYAVDRDSGMNSDITYQITARQPAWPSPFQIDSHSGNITLANRLAGGDYGMHHLFIVVRDGGKPEPLQATVWVNLLINETLEQCYLTSVPPYPSQTTEPPVFSQLEPLSKPAQSCTEPPWLVLLCGLGMMVFSACMLLAATVIFVRHKNLTRKQTSKREEITPHKLFVKLCYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JEQ2</accession>
    <name>A0AAR2JEQ2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Murine retrovirus integration site 1 homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JEQ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057416.1">
        <property type="protein sequence ID" value="ENSPNAP00000050493.1"/>
        <property type="gene ID" value="ENSPNAG00000014500.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063722"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019934">
        <property type="term" value="P:cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008677">
        <property type="entry name" value="MRVI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15352:SF2">
        <property type="entry name" value="INOSITOL 1,4,5-TRIPHOSPHATE RECEPTOR ASSOCIATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15352">
        <property type="entry name" value="LYMPHOID-RESTRICTED MEMBRANE PROTEIN, JAW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05781">
        <property type="entry name" value="MRVI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1094"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="64"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="372"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="686"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="898"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="971"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1028"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
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            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
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        </location>
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            <end position="350"/>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050493.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JGL6</accession>
    <name>A0AAR2JGL6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proline rich 5a (renal)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PRR5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PROTOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JGL6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084276.1">
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        <property type="gene ID" value="ENSPNAG00000030209.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063981"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:TORC2 signaling"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428:SF4">
        <property type="entry name" value="PROLINE-RICH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF08539">
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="49396" checksum="A072D581CC3FCA0F" modified="2024-11-27" version="1">QNVCEREREREREREREISSISGSLSHPLTMRTLHRLKLMSSPSLSELGKSEKAALEERGTQQCRAGANATWNSIHNAVIAVFQKKDLGDNELYSLNEGVRQLLKTELGSFFTEYLQNQLLTKGMVILRDKIRFYEGQKLLDSLAETWDFFFCDVLSMLQAIFHPVQGKEPSVRQLALLHFRNIITLNLKLEDALSRPRARVPPSIIQMLLILQGVHESRGVSEDYLKLEALIQKVVSPYLGTHGLYTDDGSVAQCSCVLEKRLMRRWPKPGDPPAKNPVVRSKSYNIPMLTPVAEYDTETASVGSVGVRRHSVCEMTSCIEEPAFSSVPSSPETENASLLTASRPNLSTEQDLASCAGQIEDSSPPLSPPICPECSPGPFGPGSSRDSGSDPGQEASLPSGLSSPETIVDQILESLDSDAEGIFIEFPRRCSGSSTFSRESGRQSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JH09</accession>
    <name>A0AAR2JH09_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EGF like, fibronectin type III and laminin G domains</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">EGFLAM</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JH09"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049213.1">
        <property type="protein sequence ID" value="ENSPNAP00000049141.1"/>
        <property type="gene ID" value="ENSPNAG00000013162.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158504"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000363">
        <property type="entry name" value="Agrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000032">
        <property type="entry name" value="pikachurin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000034">
        <property type="entry name" value="pikachurin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056943">
        <property type="entry name" value="EGF_Pikachurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF88">
        <property type="entry name" value="PIKACHURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25016">
        <property type="entry name" value="EGF_Pikachurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="27"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="141"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="347"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="9">
        <location>
            <begin position="387"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="561"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="9">
        <location>
            <begin position="605"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="780"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="9">
        <location>
            <begin position="831"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="806"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="983"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1012" mass="110834" checksum="A0C84A0F221ECD61" modified="2024-11-27" version="1">DLWFQIFRLGFLLWGERTLLRYLHSCPPLDIQLQTVNCTAIRVQWKMPRRHVSTITGYRVPLQPPPLPHKRIKHHSTFSLWGRDPRAEANWPVSDVVIGKLKVATHYRVSVGAYGWAGDGRPSMPRDISTASHEICMPPSPPSQPIVVAVSDTELALSWQQGESEGSAPVLHFLVAYIRPEMDTEWTYIREPVESSSMVLKGLIPETQYQFVIRAVNTLGVSPPSPINPPVRTLGAADVGSGDYDLYLMNSKTKDEDGFDVDDSDYDVFIEEVQGITPAPKSSNFTSPISVWTLTTTSGPSALTTTGSTTTTTSAVTRRISPAPSATAPPTPSPSMTVWKGAEPRVYDVPCDETVCPPNSFCINDYDSGGSRCHCNLGYQGELCVSIQFPKFYGYSHMTFEPLKNSYQSFQITVEFKADSEDGLLLYCGENDHGRGDFTSLALLRGKLHYRFNCGTGAAQIVSESSIVIGQWHTATLFRDGLNGWLRLDNDTPVSGRSQGQYSKITFRTPLFVGGAPSAYWLVRAVGTNRGFQGCVQSLSINSKPTDLRPWPRGNALSGCSQNVCEEVICANGGVCFPTRADSYICLCPLGYRGVHCEESFLLALPQFNESLQSYASAPWPQSSHTYLSFMEFEIIFQPTNQDGTLLYSDDTASQDFLSINLVGGYVEFRFDCGSGPAIIRSEEPVSMNEWHELRVSRTAKNGILQVDEQRPVEGSAEGAFTQIKCSSPLYIGGVPDYDATKSSAGVLRAFSGAIQKITLNDRAVAVATGQAGGINVLNALHPCVENPCANGGTCRPKRDGYDCDCALGYDGKHCQKECGNYCLNTVTEAIEIPQFTGRSYLTYDSRDILKRVSGSRTNVFMRFKSTSADGLLMWRGSNPMRANSDFLSVGLQDGSLMFSYNLGSGAAVIVVNGTFSDGKWHRVKAVRDGQFGKLTVDDYGAKTGRSPGKMRQLNINGDLYVGGMKEIALYTSRQYLAGLVGCVSHFTLSTDYHVSLVEDAADGKNINTCTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JI23</accession>
    <name>A0AAR2JI23_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tsukushin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TSKU</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017580878.1">
        <property type="nucleotide sequence ID" value="XM_017725389.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JI23"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068897.1">
        <property type="protein sequence ID" value="ENSPNAP00000049506.1"/>
        <property type="gene ID" value="ENSPNAG00000033622.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108444298"/>
    <dbReference type="CTD" id="25987"/>
    <dbReference type="GeneTree" id="ENSGT00940000160984"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044017638" description="Tsukushin" evidence="3">
        <location>
            <begin position="35"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="39271" checksum="A82BD3AE06C1E2BC" modified="2024-11-27" version="1">MQSNGHGAQRRRSVSTMVALLCLTLPSLLALVTAGEVKNCHPQCRCEIESYGLFDSFSLTKVDCSGVGPGTAPVPIPLDTSHLDLSHNAIGSITDSMLSGPGYTTLVSLDLSNNLITRVSPKAFSKLRYLETLDLSQNALESLGDGCFSGLPLADVDLSDNMFQNFNLDLFTTKGQETPISLDLSNNHLTTVSRNPQAHAPFIKSLMLAGNRLQTVPKLAGIPLQYLNLDGNLIPIIEEGAFEEMKDLIYLSLSGLLELSAIQPSSFRGLSNLQVLDLSNNRQLKGISPDVFNGLTSLQELNLSQSVVTPLPATIFNLMPSIKSVSLGPNMHCWKTHKQGQFHRQIGQAKANDILTCDVAGIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JIB5</accession>
    <name>A0AAR2JIB5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JIB5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042922.1">
        <property type="protein sequence ID" value="ENSPNAP00000051705.1"/>
        <property type="gene ID" value="ENSPNAG00000019607.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155816"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000008">
        <property type="entry name" value="Ras-GEF domain-containing family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000007">
        <property type="entry name" value="Ras-GEF domain-containing family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF197">
        <property type="entry name" value="RAS-GEF DOMAIN-CONTAINING FAMILY MEMBER 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="4">
        <location>
            <begin position="105"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="275"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="62257" checksum="91A6784C8761CEC7" modified="2024-11-27" version="1">MAKFLAELLGCTLPEKGASPLFEVREPVKQSELTGVLCPAACSSFNQLWSQGSWCLSSNPVVDTPGERCRQMPQTPPFPGMFASSGYNKNLYQTKEEDYGGLYYHDNNLVSGSLEALIQHLVPTVAYYPDRTYIFTFLLSSRLFIHPYELMSKVCHLCVDHQRLSDPQADKVRIRKIAPKILQLLTEWTETFPYDFRDERMMRSLKELTHRLSTGDELYRKTVQQMTQALIRKLATLSQYEEALAKINSTVTDRLTVLKTKPQSIQRDILTICNDPFTLAQQLTHIELERLSYIGPEEFVQAFVQKDPLDNEKSCFSDHKKASNLEAYVEWFNRLSYLVATEICMPVKKKHRARVIEFFIDVARECFNIGNFNSLMAIISGMNMSPVSRLKKTWGKVKTAKFDILEHQMDPSSNFYNYRTALRGATQRSITAHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVREFMTWKKVECPFERDRKILQYLLTAPVFSEDALYLASYESEGPENNMEKDRWKSLRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JJ21</accession>
    <name>A0AAR2JJ21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase</fullName>
            <ecNumber evidence="11">2.4.2.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GXYLT2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Glycosyltransferase which elongates the O-linked glucose attached to EGF-like repeats in the extracellular domain of Notch proteins by catalyzing the addition of xylose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-O-(beta-D-glucosyl)-L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-[alpha-D-xylosyl-(1-&gt;3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56064"/>
            <dbReference type="Rhea" id="RHEA-COMP:14610"/>
            <dbReference type="Rhea" id="RHEA-COMP:14611"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:140575"/>
            <dbReference type="ChEBI" id="CHEBI:140576"/>
            <dbReference type="EC" id="2.4.2.42"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.42" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017538035.1">
        <property type="nucleotide sequence ID" value="XM_017682546.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JJ21"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061256.1">
        <property type="protein sequence ID" value="ENSPNAP00000049861.1"/>
        <property type="gene ID" value="ENSPNAG00000033760.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411137"/>
    <dbReference type="CTD" id="727936"/>
    <dbReference type="GeneTree" id="ENSGT00940000158065"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140563">
        <property type="term" value="F:UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06430">
        <property type="entry name" value="GT8_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000042">
        <property type="entry name" value="Glucoside xylosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051993">
        <property type="entry name" value="Glycosyltransferase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46012:SF1">
        <property type="entry name" value="GLUCOSIDE XYLOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46012">
        <property type="entry name" value="IP22168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043703339" description="UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase" evidence="14">
        <location>
            <begin position="29"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="433" mass="50108" checksum="149BCB52AB4F86E2" modified="2024-11-27" version="1">MRIHCRVAAIALCLGLFLLLYFLGGNGADEPPLREVEKKDEQFPPSRVTKQSRGEPQQEVRADRKEPRISGRGASVVARLRQKSELGRSKVTRAEDVMHLAVVACGNRLEETLTMVKSALLFSIKKIKFHIFAEEDLTEQFEKALSQWPRPLTSKFQYSLYPITFSVGNAEEWKRLFKPCAAQRLFLPVILKDVDSLLYVDTDVLFLRPMDDIWKFLKAFNSSQLAAMAPEHEIPKIGWYSRFARHPFYGVTGVNSGVMLMNLTRIRSTMFKNSMIAGGLSWEDLLHPLYQKYKNHITWGDQDLLNIIFHYNPECLYIFPCQWNYRPDHCMYGSNCKGAEEEGVSVLHGNRGVYHDDKQPAFKVVYDAIRDYPFGENLFQSLFYPLQTKFLDTVNTLCGRIPQVFLKQIEKTMKAVFEQKVIRHARAPRRQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JPN3</accession>
    <name>A0AAR2JPN3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyl-accepting transducer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NFYC</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JPN3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084839.1">
        <property type="protein sequence ID" value="ENSPNAP00000053973.1"/>
        <property type="gene ID" value="ENSPNAG00000036234.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174258"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Methyl-accepting transducer" evidence="3">
        <location>
            <begin position="486"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="309"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="443"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="755"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="907" mass="105895" checksum="64A817BFCD9A76C9" modified="2024-11-27" version="1">MVDRKANGGTSGCRKSTVRTNTTKHRTSHREHRTSHRTPHREHRTSHRTSHREHRTSHRTPHREHRTSHRTSHREHRTSHRTPQRTQDITQRTQDTTQRTQDITQDTTQRTQDITQRTQDITQGTQDITQRTQGTQDITQRTQDITLRTQDTTQRTQDITQGTQDITQRTQGTQDITQGTQDITQRTQDITQGTQDITQGTQDITQGTQDTTQRTQDITQGTQDITQGTQRTQGTQDTTQRTQDITQRTQDITQGTQDITQGTQRTQDTTQRTQDITQGTQDITQGTQDITQRTKDITQGTQDITQRTQDITQRTQRTQDITQGTQDITQRTQRTQDITQRTQDITQRTQRTQDITQRTQDTTQRTQDITQGTQDITQRTQDITQRTQDTTQRTQDITQGTQDITQRTQDTTQRTQDITQRTQRTQDITQRTQDTTQRRQDTTQRTQDITQRTQRTQDITQRTQDITQGTQDTTQRTQDITQRTQRTQDITQRTQDTTQRTQDITQGTQDITQRTQDTTQRTQDITQGTQDITQGTQDITQRTQDTTQRTQDITQRTQDITQRTQRTQDITQRTQDTTQRTQDTTQRTQDITQRTQDITQRTQDITQRTQDITQGTQDTTQRTQDITQRTQDTTQRTQDITQRTQDITQRTQDTTQRTQDITQGTQDITQGTQRTQDITQRTQDTTQRTQDITQGTQDITQGTQDITQRTQRTQDTTQRTQDITQRTQDITQRTQRTQDITQRTQDTTQRRQDTTQRTQDITQRTQDITQRTQDTTQRTQRTQDITQRTQDITQGTQDTTQRTQDITQRTQDITQRTQDTTQRTQDITQRTQDITQGTQDTTQRTQDITQRTQDITQRTQGSGSTECYIGNIGPYIWNTGFYIENTGPCGFPCLVSLIDLIMWFLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K7Y9</accession>
    <name>A0AAR2K7Y9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K7Y9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050548.1">
        <property type="protein sequence ID" value="ENSPNAP00000060390.1"/>
        <property type="gene ID" value="ENSPNAG00000034464.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286933"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13384">
        <property type="entry name" value="HTH_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="66"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="145"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="38113" checksum="67978DFF764DB052" modified="2024-11-27" version="1">MGKQRDLSTGIVAQIQALRQQGLTQTKIAEQLGISQSSVCKALKKNCSKRTNCSGVRKTSARDNKQLRKIAVSNRFKSTSELTDLRNKQTGADVSRSTTYHRLRELGFKSRVPAVKPMLNKKQMEKRLKWAKEHSEWTAEDWQKVVFSDESRFCISFGDQGPRVWRRGGETYNHECVKRSVKFPQSVMVWGCMSARGVGKLCFLKKTVNAAVYQDILETFLIPTVEEQFGEEDFIFQQDLAPAHAAKSTKDWFTKKQLEVLAWPANSPDLNVIENLWAIVKRKIRDRKPTTLDQLKQNIATAWEAVSAETCDKLVKSMPRRLQAVIQAKGAATKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K923</accession>
    <name>A0AAR2K923_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K923"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065976.1">
        <property type="protein sequence ID" value="ENSPNAP00000058591.1"/>
        <property type="gene ID" value="ENSPNAG00000031882.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18659" checksum="6596D28796D32C0B" modified="2024-11-27" version="1">CNSKGMQWIRKRRGNPYSLLTFMLPKADGCGTGLQLSAKEVVLGRPDIIPSLPKRKSSRYPSEFAYYCCEMISDRTRWDPFISELNYLSNKKMEEGRPRTLSSTPVHLGLVFLSDHLLFSCRWSPLLQHGIHVCQWNEVMKPAFFPVSPRTTTKQTCRLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KF82</accession>
    <name>A0AAR2KF82_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LENG1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017537317.1">
        <property type="nucleotide sequence ID" value="XM_017681828.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537318.1">
        <property type="nucleotide sequence ID" value="XM_017681829.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537319.1">
        <property type="nucleotide sequence ID" value="XM_017681830.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KF82"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081420.1">
        <property type="protein sequence ID" value="ENSPNAP00000062945.1"/>
        <property type="gene ID" value="ENSPNAG00000032684.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108410645"/>
    <dbReference type="CTD" id="79165"/>
    <dbReference type="GeneTree" id="ENSGT00510000048131"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039875">
        <property type="entry name" value="LENG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER LRC MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093:SF0">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="275" mass="32737" checksum="88ED98EC25738DDF" modified="2024-11-27" version="1">MNILPKKSWHVRNKDNIARVRRDEAQAAEEEREIQRRAERAEQEARTEYLRKKSRVALEQRGGYREEGEQSGGSGGIEHLNLFPLEESSEKKGNAEYLKEKREEKEKQERAIGLLVSLGPAPGTEATPWYLKHREKEKEEDRDRKEKGKRKSITEEEREKKERKLKERLDPLLDMKKALAVKDRKEKKHKKKEKRDRGEKRSGESSIERLRAERLQREADERRRTQALLDQRNGGGEEGKRQKEMGDRDRPYNSAYFPELARKRQRKERDQYDFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KFW9</accession>
    <name>A0AAR2KFW9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Relaxin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a B chain and an A chain linked by two disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the insulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017579403.1">
        <property type="nucleotide sequence ID" value="XM_017723914.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KFW9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077254.1">
        <property type="protein sequence ID" value="ENSPNAP00000061337.1"/>
        <property type="gene ID" value="ENSPNAG00000032833.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108443329"/>
    <dbReference type="CTD" id="796288"/>
    <dbReference type="GeneTree" id="ENSGT00940000154396"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04365">
        <property type="entry name" value="IlGF_relaxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022352">
        <property type="entry name" value="Ins/IGF/rlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016179">
        <property type="entry name" value="Insulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051777">
        <property type="entry name" value="Insulin-like_neuro_ligands"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036438">
        <property type="entry name" value="Insulin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022353">
        <property type="entry name" value="Insulin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20968">
        <property type="entry name" value="ILGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20968:SF0">
        <property type="entry name" value="RELAXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00049">
        <property type="entry name" value="Insulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00276">
        <property type="entry name" value="INSULINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00078">
        <property type="entry name" value="IlGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56994">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00262">
        <property type="entry name" value="INSULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043927483" description="Relaxin-3" evidence="12">
        <location>
            <begin position="22"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Insulin-like" evidence="13">
        <location>
            <begin position="30"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="69"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="155" mass="16759" checksum="F1429942A57DF214" modified="2024-11-27" version="1">MYKEVVLTLCLLVALASRVQATDGHPIYGVKLCGREFIRAVIFTCGGSRWRRSIGMAADLSSDLLSAHDDEASDGWGSNPISALSYKPHSDSETPNWAPESPEGPTFPRPARSLISDEVLEALRTSDRKGRDVVVGLSNACCKWGCSKSEISSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KPM3</accession>
    <name>A0AAR2KPM3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="18">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="18"/>
    <dbReference type="RefSeq" id="XP_037391797.1">
        <property type="nucleotide sequence ID" value="XM_037535900.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000070436.1">
        <property type="protein sequence ID" value="ENSPNAP00000064362.1"/>
        <property type="gene ID" value="ENSPNAG00000015239.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415490"/>
    <dbReference type="CTD" id="436745"/>
    <dbReference type="GeneTree" id="ENSGT00940000165254"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02081">
        <property type="entry name" value="P-type_ATPase_Ca_PMCA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000001">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000002">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000008">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000001">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000002">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000007">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022141">
        <property type="entry name" value="ATP_Ca_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006408">
        <property type="entry name" value="P-type_ATPase_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01517">
        <property type="entry name" value="ATPase-IIB_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF284">
        <property type="entry name" value="PLASMA MEMBRANE CALCIUM-TRANSPORTING ATPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12424">
        <property type="entry name" value="ATP_Ca_trans_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 18" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="440"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="952"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="990"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1028"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="50"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1229"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="1099"/>
            <end position="1126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1296" mass="143121" checksum="AA2A2C70E2CFACB3" modified="2024-11-27" version="1">MGDMANSSVDFHPKKPGMDGKACAEDFEVSLEELRALMEFRGAEALQKIQESYGDTEGLCHRLKTSPTDGLSDDPADLEKRWQVFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYQPPGGDSEVCGNVSGGMEDEGEAEAGWIEGAAILLSVLCVVVVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNGTVLQIPVAEMVVGDVAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKAIDRDPMLLSGTHVMEGSGKMVVTAVGINSQTGIIFTLLGAGDVEEEKKENKKAEVSSSSSVQFAAAPAKECSVVNGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEREKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIETFVVQDRPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTAVQIYIGDQHFHEIPTPQHISHHALELLTTAISLNSAYTSKILAADKEGGLPKQVGNKTECALLGLVLDLKRDYQCVREQIPEEKLYKVYTFNSVRKSMSTVVQLPDRSFRIYSKGASEILLKKCSSVLSRDGGAHPFRPRDRDEMVKKVIEPMACDGLRTICVAYRDLTADPLPEWDNEADIINNLTCIAVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIQAGDDFLCMEGKDFNRRIRNEKGEVEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTICEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHGVYQLIIIFTLLFVGERIFDIDSGRDAPLHAPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCTIVLGTFAIQIAIVQFGGKPFSCSPLNVEQWLWCLFVGVGELLWGQLISSVPTSQLKCLKEAGHGPAPDDIMDEDLAEDEEEIDHAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDIRVVKAFRSSLYDGMEKPESRSSIHDFQAHPEFIITDSGHSIPLIDETDVDDESERSNHNHVRVHLRHGPLPASSLPPQRPPRSRYPTRQQSLPVTLNCNNNAAESRVYLTRPAHVPGPPSPMHSFETCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KRL2</accession>
    <name>A0AAR2KRL2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Syntaxin binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">STXBP2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KRL2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083036.1">
        <property type="protein sequence ID" value="ENSPNAP00000065042.1"/>
        <property type="gene ID" value="ENSPNAG00000016096.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160045"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="68715" checksum="389FD172767830FB" modified="2024-11-27" version="1">ISDFYQVLELILKKIVLSKQKVLISPLFSEIINGVLKSIKKDGEWKVLIVDHIGMRILSSCCKMSDIMAEGVTIVEDINKRREPISSLEAIYLISPVQKSVQALVSDFKDSAFTYKAAHIFFTDTCPDGLFAEVAKSRVSRVIKTLKEINVAFLPYESQVFSLDDPRSLELFYSPTRTNQDERTKMMEAVAEQIATLCDTLKEYPAIRYRQGRKENAELAEMVLDRLNAHKADNPSMGEGTDKARSQLLIVDRGYDPISPLLHELTFQAMVYDLLEIDQDIYKYETTGLGENKTKEVLLDENDELWVQLRHMHIADVTKKVTELLRTFCESKRMNTDKANIKDLSQMLKKMPQYQKELGLYSTHLNLADACMKKFKASLDKLCEVEQDLAMGSDAEGEPIKDAMKSIVPVLLNKEIQPYDKIRIILLYIFHKKKGISEENLTKLIQHADVQQDKNIITSMQHLGCPIIAGSLPERKERTESTYQLSRWTPILKDVMENLIEDKLDKKQWPFLSDPAPITTTQTAVSARFGHWHKNKTATEYCSGPRLIIFVIGGVTHSEMRSAYEVTRATEGKWEVVIGSSHVLTPTSFLNDLKKLDQPDQASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KT30</accession>
    <name>A0AAR2KT30_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Collectrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017574119.1">
        <property type="nucleotide sequence ID" value="XM_017718630.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KT30"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081531.1">
        <property type="protein sequence ID" value="ENSPNAP00000065654.1"/>
        <property type="gene ID" value="ENSPNAG00000033897.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108439956"/>
    <dbReference type="CTD" id="57393"/>
    <dbReference type="GeneTree" id="ENSGT00940000160862"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051957">
        <property type="term" value="P:positive regulation of amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042944">
        <property type="entry name" value="Collectrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031588">
        <property type="entry name" value="Collectrin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46884">
        <property type="entry name" value="COLLECTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46884:SF1">
        <property type="entry name" value="COLLECTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16959">
        <property type="entry name" value="Collectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52010">
        <property type="entry name" value="COLLECTRIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043647332" description="Collectrin-like domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Collectrin-like" evidence="11">
        <location>
            <begin position="26"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="218" mass="24297" checksum="38242C47D5AC46E6" modified="2024-11-27" version="1">MWTVWTVWMVWMVCLVPTTSEALCTEDDGYKVRLSIKTALGGDAYEWNESEMFFFKATLAFAMRTYSGGEQYDVSNILVCDETLRVSFVFVVTKPSNTSELVPRTEVENAIKKSRHRINSAFLLTDQTLDFVGINPTLAAPIQYDTPPWLIAFGVVMGLVCAGIVALLVSTFLNKKRSKKSADEEEAGRGNGILCESIRGKDGLFNQGLVDDDRLTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KY59</accession>
    <name>A0AAR2KY59_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KY59"/>
    <dbReference type="Ensembl" id="ENSPNAT00000088164.1">
        <property type="protein sequence ID" value="ENSPNAP00000069205.1"/>
        <property type="gene ID" value="ENSPNAG00000011845.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234574"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF10">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="17">
        <location>
            <begin position="231"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="442" mass="50286" checksum="0E505BD74085C9AC" modified="2024-11-27" version="1">MGGFWAAVVPVLQMFFLSLLLLIMLPAMFGFSLGITETYMKILIKTLKWATLRIQKSSKEEETLKHSSSNGLIQRDDSSLEKEIGERRGNRRGDFALSDVFYFSKKGIESIVEDEVTQRFSSEELVSWNLLTRTNNNFHYISLRLTILWGVGVIVRYGILLPLRIFYDIILSYCWMNPQLACCNDGVDALHCRLKDWLSELVHVMCYRICARGLSATIHYHNRENKPKKGGICVANHTSPIDVVILANDGGYAMVGQVHGGLMGVVQRAMVRACPHIWFERAEMKDRHLVTQRLRDHVNDKKKLPILIFPEGTCINNTSVMMFKKGSFEIGGTIYPVAIKYDPQFGDAFWNSAKYNMVSYLLRMMTSWAIVCNVWYLPPMTQKEGEDAVQFANRVKSAIAQQGGLVDLSWDGGLKRAKVKDTFKQEQQKKYSCMVVGDDSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L040</accession>
    <name>A0AAR2L040_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">10-formyltetrahydrofolate dehydrogenase</fullName>
            <ecNumber evidence="9">1.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ALDH1L2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-10-formyltetrahydrofolate + NADP(+) + H2O = (6S)-5,6,7,8-tetrahydrofolate + CO2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10180"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="1.5.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10181"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">In the N-terminal section; belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.6" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L040"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047932.1">
        <property type="protein sequence ID" value="ENSPNAP00000067766.1"/>
        <property type="gene ID" value="ENSPNAG00000010833.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158018"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016155">
        <property type="term" value="F:formyltetrahydrofolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009258">
        <property type="term" value="P:10-formyltetrahydrofolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07140">
        <property type="entry name" value="ALDH_F1L_FTFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08703">
        <property type="entry name" value="FDH_Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08647">
        <property type="entry name" value="FMT_core_FDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.25.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000008">
        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000009">
        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="entry name" value="Formyl transferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011407">
        <property type="entry name" value="10_FTHF_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036489">
        <property type="entry name" value="10-FTHFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 12" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="16">
        <location>
            <begin position="340"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="697"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="697"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="731"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="595"/>
            <end position="597"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="621"/>
            <end position="624"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="654"/>
            <end position="659"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="674"/>
            <end position="675"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="781"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="828"/>
            <end position="830"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="13">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="926" mass="101641" checksum="92C8F3629673395F" modified="2024-11-27" version="1">SSKRGSFCLPISCFFSQAYYQNKLKLALIGQSLFGQEVYTNLRKQGHKVVGVFTVPDKDGKADPLAVAAEKDGTPVFKFPRWRVKGKPISEVVDTYKAVGAELNVLPFCSQFIPMNVIDHPKHGSIIYHPSLLPKHRGASAINWTLIEGDKKAGFSIFWADDGLDTGPILLQKECAVEPSDTVDTLYNRFLFPEGINSMVEAVQLIADGKAPRIPQPEEGASYEGIQKKSNAKVSHTHPAEVIHNWIRGHDKVPGAWAVLDGQPVTLYGSSLLSSPVPAGQPLEVEGASQPGLITSSGLVLFGSDGKALQVKSLQFEDGKMIPAAKYFSSGDTTSIELTEEEKKMAEEIRVIWKGILSNVPVIEDTTDFFKSGAASMDVVRLIEEIKPKCADVQLQNEDVYMATSFHDFIQMFVRRLRGEDQEEELVINYVSATKDINNMTLKMPYQCFINGKFEDAENGKTYDTVNPTDGSVICKVSFASVADVDRAVAAAKEAFETGPWGKMNPRDRGRLLYKLADLMEEHQEELATIEAIDSGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGTTIPINQARPNRNLTFTKKEPLGVCAIVIPWNYPLMMLAWKSAACLAAGNTLVLKPAQVTPLTALKFAELTVKAGIPKGVINIIPGSGGLVGQRLSDHPDIRKLGFTGSTPIGKQIMKSCAVSNLKKVSLELGGKSPLIIFSDCDMDKAVRMGMSSVFFNKGENCIAAGRLFVEESIHDEFIARVVEEIKKMKIGDPLDRSTDHGPQNHKAHLDKLVEYCDIGVKEGATLVYGGKQVDRPGYFMEPTVFTDVEDHMFIAKEESFGPVMVVSRFKDGDVDGVLSRANDTEFGLASGVFTRDINKAMYVSERLEAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEEALHEYLRTKAVTVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L164</accession>
    <name>A0AAR2L164_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L164"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050367.1">
        <property type="protein sequence ID" value="ENSPNAP00000068367.1"/>
        <property type="gene ID" value="ENSPNAG00000003071.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000067">
        <property type="entry name" value="Olfactory receptor C family, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="620"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="691"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="735"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="780"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="815"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="847"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="621"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="890" mass="99191" checksum="133701460E62F178" modified="2024-11-27" version="1">MQRNRWTTVCNCRTTKCSYTVSGADKMDTEHGNKEVVLMFCLIRFMKQWMASVMFFHVILLFLSLFAKGEKPLCNIMESPEYPLLSKDGDVTIGGAFSIHSSVMMPSMLFAEKPEPLICSSLNLRELRFAQVMIFVIEEINRDSSLLPNISVGYRIYDNCGSRFLSMKAAMALMNGQELTAIEGCSGQAGVQAIIGESESSPTIVLSRTTGPFKIPVVSHAATCECLSSRTEYPSFFRTIASDYYQSRALAQLVKHFGWSWVGAVNSDSDYGNNGMAIFLEAAEEEGICVEYSEKFDRSEPAKLMKVVDIITKGTAKVIIMFVAHIEMNILVEQLILKNVTGFQMIGVEVWITAVNLITPTSYSLLGGSLGFDVGKSNIVGFADYVVKEFWETTLPCWVTGGNTPQAENNCSRYQDLFPFKNYNEDVSELRYANNIYNGVYAVAHSLHSLLKCTEKQGCEKDQTIQPWQVVESLKKVNFTTKTGEQVWFDSTGATAAKYDVINWQRGPDGEVLFKQVGYYDASLPPGQQFVITDKNIVWGGGKTEKPRSVCSESCPPGTRKAAQKGRPVCCYDCIPCAEGEISNMTDSNNCEPCPGEYWSNAERDKCVLKVVEFLSFTEVMGILLVFFSLFGTGLTMLVGILFLIKKDTPIVRANNSELSFLLLFSLTLCFLCSLTFLGRPSEWSCMLRHTAFGITFVLCISCVLGKTIVVLMAFRATLPGSNAMKWFGPPQQRLSVLAFTLIQVLICIIWLTILPPFPYNNMHYYKEKIILECRFGSAVGFWAVLGYIGVLAILCFVLAFLARKLPDNFNEAKFITFSILIFCAVWITFIPAYVSSPGKFTVAVEVFAILASSFGLLFCIFTPKCYIILFKPELNTKKHVMGKTASKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4R4</accession>
    <name>A0AAR2L4R4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L4R4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043933.1">
        <property type="protein sequence ID" value="ENSPNAP00000069642.1"/>
        <property type="gene ID" value="ENSPNAG00000015658.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163148"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF98">
        <property type="entry name" value="CRYGM5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="8"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="47"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="138"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="185" mass="22123" checksum="54ED7425DB44D3CB" modified="2024-11-27" version="1">RHGISEFLQVIFYEDRNFQGRSYECSSDCSDVFTYLSRCNSCKVENGCFMVYDDSNFMGNQYFIKRGEYSDYTCMGMRDSIDCIRSCRMIPMHRGTFRMRIFERENFSGQMHELMDDCDSIQKRYRMSDCQSCNVMEGHWLLYEQPDFRGRMIYLRPGEYRSLRDMGVGPMNFRIGSLRRIMEPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L5K9</accession>
    <name>A0AAR2L5K9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L5K9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069180.1">
        <property type="protein sequence ID" value="ENSPNAP00000071893.1"/>
        <property type="gene ID" value="ENSPNAG00000032057.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166867"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF296">
        <property type="entry name" value="PROTOCADHERIN 1 GAMMA 11 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="691"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="62"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="138"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="247"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="352"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="457"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="581"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="832" mass="91855" checksum="4CCC28CC0B2ECD92" modified="2024-11-27" version="1">MLVFLLHFSSTMQLKWQTVCFFLCIYGMDVVFCQARYSIPEEMPEGSFVGNIAKDLGIEISRLISGKARVVTKGGRQYVELSRDKGALVVKERIDREELCKQTTPCSFSFDLIIENPIQLHRITVEVQDINDNAPIFPKGAVNLEISENRLSGARFPLDSAVDMDVGINGIQSYALSPTDNFKLEINSQSDGSKHVEMVLQRELDREEHDELYLVLIAADGGSPRKSGTVRIHVTVLDVNDNAPVCKQAVYKIDVMENSPVGTVLTTVNAVDADEGINGDISYSIAQASKEAREMFDIDSDTGDITMIKMVDYEIERSYSLNIKAKDKGSLSDTCKVIIDVVDVNDNPPTIQLMSFSNTIAENSPVGTTVAVINVEDADSGQNGVVQCKINEHIPFKIESSLTDYYALVTDEILDRENIAEYNITILVSDQGSPALHSNKTLTVKISDVNDNAPVFSSDQFRTFITENNSPGVAVLTVKAGDADWGPNARLSYFLEDSDVMGHPVSSLVSINSDSGVINAVRSFDYEQMKSLTFNVTARDGGSPPLSSEVMVTISVQDQNDNAPQVLYPVQTGVSVVAEIVPRSADVGYLVTKVVAVDVDSGQNAWLSYKLQKATDRALFEVGPQNGEIRTVRQVTDKDAVKQKLTVVVEDNGQPSRSAVVNINVAVADSFPEVVSEFSDFTLSKEYNDNLTFYLVLALAAVSFLLITTVVVIISVKIYRWRQSRIFYQSSLPVIPYYPPGYADTGVTGTLPHMYNYDVCMTTNSRKSDCKYSTLGGQSVLVVDQSFSETLQRAMKEKEFLENAESPEMVEIQIRNFKNVLLTDEMSKLFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LCH6</accession>
    <name>A0AAR2LCH6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NPC1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>cholesterol(in) = cholesterol(out)</text>
            <dbReference type="Rhea" id="RHEA:39747"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000048995.1">
        <property type="protein sequence ID" value="ENSPNAP00000074308.1"/>
        <property type="gene ID" value="ENSPNAG00000012527.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156182"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
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    <sequence length="1298" mass="145274" checksum="0776147370236859" modified="2024-11-27" version="1">MASGSLPREVFQTCLTGRRPWGRPRTRWRYYISRLSWCLGIPLEELEEAQAQHCIWYGECSNSTILPEKKYNCNYTGPPKPLPKDGQGLLQELCPGLVYGNQSVCCDTQQLKTLHSNIQLPLQYLSRCPACFFNFMTLFCELTCSPHQSQFLNATEFSSDPVDNRTNVVKLSYYISNMFANAMYNACKDVEAPSSNVKALSLLCGRDASQCNPTNWIQYMFDIKNGQVPFAIDPVFEDDPVSGMTPMGNHTFDCTEPLDDGSGPCSCQDCSKACGPKPVPPPTPPPWTILGLDAMNIIMWSSYMAFLFVFITALLGAWCCRKRTITSEYGPIQDSNQPHSLNDSVTCCESLRENFANALHYVFSLWGSFCVRQPLLVIMLSMVLVAACSTGLMHMRVTTNPVDLWSAPHSEARQEKDYFDQHFGPFFRTEQLIITTPWTEWFKLVSTTGPDILFAPILNISLLQQVLDLQTDIENLEAEYKGQKVTLKDICVSPLAPYNNNCTILSVLNYFQNSHEVLNHTFADEFFIYADYHTHFLYCVSSPVALDDMGHFHDPCMGTFGGPVFPWLVLGGYEGTAYNNATALVITFPVNNYLNDTDKLGKVLAWEKEFISFMKNYSNPNLTISFSSERSIEDEIDRESNSDVGTIIISYVIMFVYVSMALGNIHSFRRLLVDSKISLGIAGILIVLSSVACSLGIYSYAGVPLTLIVIEVIPFLVLAVGVDNIFIMVQAVQRDERMQHEELHQQIGRVLGDVAPSMLLSSISETVAFFLGSLSHMPAVKTFSFFAALAILIDFLLQISCFVSLLGLDMKRQERNRLDILCCIKLPEGQQEKTEGLLFRFFKKVFAPFVLKEWVRPLVVALFVGMLSFSIAVTDKVEIGLDQRLSMPDDSYVLDYFGNLTEYLHTGPPVYFVVREGHDYRTSYGQNQVCGGVGCNNDSLVQQVYTASLMSDYSKISTTPSSWLDDYFDWVKPQSTCCRYYNATGAFCNASVVDPSCVRCRPMTPSGKQRPNGTEFMKFLPMFLSDNPNIKCGKGGHAAYSTAVVLKDNNTNVGATYFMSYHTILKTSSDFIDAIKIARELAYNISVSMGLENKTHFVFPYSVFYVFYEQYLSIAHDTALNLSMCLVAIFVVTTVLLGFELWSAVLVSLTIAMIVVNMFGVMWLWGISLNAISLVNLVMSCGISVEFCSHIVRAFSISTKSTRVERAEEALAHMGSSVFSGIMLTKFGGILILALSKSQIFQVFYFRMYLAIVLLGATHGLIFLPVLLSYAGPSVNKAKVMTTRSRFSGTERERLLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LEP0</accession>
    <name>A0AAR2LEP0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LEP0"/>
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        <property type="protein sequence ID" value="ENSPNAP00000075063.1"/>
        <property type="gene ID" value="ENSPNAG00000036710.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159653"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16254">
        <property type="entry name" value="EFh_parvalbumin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Parvalbumin, thymic"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PARVALBUMIN ALPHA"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="249"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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            <name>Ca(2+)</name>
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            <label>1</label>
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            <name>Ca(2+)</name>
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            <label>1</label>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
        </ligand>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <location>
            <position position="268"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
        </ligand>
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            <position position="273"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
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        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023179"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="280" mass="30483" checksum="855C737CCD0F76FC" modified="2024-11-27" version="1">IYHSLSDSVHYHSRSASLSTSFSLLKLYHFLSCFHYLSLCHFFSSSLSHPHTLSLPLCLSLFLSSLPPCFPLSFSRSLSPLHRTSNQSALPLPPALSLSLSFSLSVSPSSSSFSLLSSFSLSFVSLFLSSSQWVLDILAACVHTSIRHALAQDRPAAALKPGHKHSTPAAEMAMSSILNADDIKKALDAFKAADSFDHKKFFEIVGLKAKSADDVKKAFHVLDADNSGFIEEEELKFVLKGFATDGRDLTDKETKAFLQAADKDGDGKIGVDEFAALVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LES9</accession>
    <name>A0AAR2LES9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rho GTPase activating protein 45b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017556839.1">
        <property type="nucleotide sequence ID" value="XM_017701350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LES9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044199.1">
        <property type="protein sequence ID" value="ENSPNAP00000072896.1"/>
        <property type="gene ID" value="ENSPNAG00000020341.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429536"/>
    <dbReference type="CTD" id="555849"/>
    <dbReference type="GeneTree" id="ENSGT00950000183110"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20816">
        <property type="entry name" value="C1_GMIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000016">
        <property type="entry name" value="Rho GTPase activating protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054713">
        <property type="entry name" value="GMIP/FCHO2-like_FCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057028">
        <property type="entry name" value="RHG29_45_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF18">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22699">
        <property type="entry name" value="GMIP-like_FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24235">
        <property type="entry name" value="RHG29_45_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="303"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="725"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="784"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="453"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="594"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1006"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="594"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="640"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1008"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1025"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1041"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1072"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1105"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1136"/>
            <end position="1146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1146" mass="126302" checksum="99D86CB04E317EE5" modified="2024-11-27" version="1">MMKRGTGGKSSYSPYSTSQRVKKSDSKTKLDILPNRPNVWLKQLSILQEQPRKDTSDSSTSSSANPASLCPSVTDAPSCPGTPSAQHTKLVGCPSPVGTLKRPTALSRHASAAGFPLQSWVFSKGQGRAAIAQSPQAETVEMPAIIEVEDIPSLLRVVARFAEAMEKLKDVVLEDKQENRRPLAHECLGEVLRILRQVISMYPLLNTVETLTAAGKLISQVKGFHYEVCNESEKMDFEKAIETIAVAFSSNVSELLMGEVDSSTLLSVLPTEKSRSMENLYKISGQETSQTRSDSSEFVMSAEEVDILLQRSEGGVDSALMYAKTISKYMKDLIGYVEKRITLEVEFSKGLQRLYQACKQTITQPHMPFFAIYSLALEQDLEQSAGVHQAANTLHNQTFIQPLMQRKQEHEKKRKEIKELWLKAKRKLMESEANLKRAKQVYVARYEEYEKAKTAASRAEEEGSSSTTKALDKKKRLEEEARNKAEEAEATYRTCIADATTQHQELEHTKVTVLRQIQEVIKQSDQTLRSATISYYQTMHVQTVALPVHYQTLCESSKLYDPGQQYATHVKDLQMSEEPEVHYSFEPYCPISSQQAARLRTDSSNTDAHSSTEASATGEDPSSSQKEEQKKARSHMTHKSWGSTVSDTDSVCGGSGLGSPTTSTGDISKMARTASTGTMSSNEDADEKDGNMSSFETSNINGIEPEIVVPTGPFRNIGLSKAAQTHRLRKLRSPAKCRQCDSYVYFQGAECEECFLACHKRCLETLAIQCGHKKLQGRLQLFGQEFSLVSNNSSDGIPFIVKKCICEIERRALRMKGIYRVNGVKTRVEKLCQAFENGKELVELSQSSPHDISNVLKLYLRQLPEPIMPFRLYNSLMGLAKESLALVGPEGAEAGKGPDLVDLGQKTDPELMVLVHRLKELLADLPKANITTLRYIARHLRRIAELEEDNKMSPSNLGIVFGPSMMRPRPSGATVSLSSLVDYPYQARIVETLIVFYRTIFPSESHHTGSTSAVTSPSLQQESSVDGEDSGTNDGDEADHTEEHGGTEAAKLSEGRDGSTGTLGSLEHSLDSDSELEEEGQVEAPILSPMDQCSSEDPGHPSAATDLQSPSGSDSEPPALPDSEPPDLHSPEEETAMSSLGDQPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LGR1</accession>
    <name>A0AAR2LGR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CYRIA/CYRIB Rac1 binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYRI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LGR1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073370.1">
        <property type="protein sequence ID" value="ENSPNAP00000073904.1"/>
        <property type="gene ID" value="ENSPNAG00000009430.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015159"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039789">
        <property type="entry name" value="CYRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009828">
        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12422">
        <property type="entry name" value="GH09096P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="5">
        <location>
            <begin position="18"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="351" mass="40475" checksum="70DFDAFAE61B1FDC" modified="2024-11-27" version="1">MGNLIKVLGKDLENCPHFFLDFENAQPTEAETAVWNQVSAVLEEAHGILAELQSYNGAGQEIREAIQNPNDLQLQEKAWNAVCPLVAKLKRFYEFSLRLENALRSLLEALTSPPYAPMQHLEREQALAKQFAEILHFTLSFDELKMTNPAIQNDFSYYRRTISRNRLNNQQLEAENEVNNEMANRMSLFYAEATPMLKTLSNATTKFVSENKTLPIEDTTDCLSTMACVCRVMLETPEYRCRFTNTDTMLFCMRVMVGVIILYDHVHPVGAFAKTSKIDVRDSEFFIYFHTFKIESILATFTLQVILPCFDFLLRFESSASTEICIRNCLFSLRLHDHKSDMYPIQENIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LHJ2</accession>
    <name>A0AAR2LHJ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NEDD8-activating enzyme E1 regulatory subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Amyloid protein-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LHJ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064758.1">
        <property type="protein sequence ID" value="ENSPNAP00000074204.1"/>
        <property type="gene ID" value="ENSPNAG00000016102.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074901"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000174">
        <property type="entry name" value="NEDD8-activating enzyme E1 regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="494"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="526"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="569"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="613"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="652"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="776"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="804"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1056" mass="113726" checksum="CF44B7D15E8390E4" modified="2024-11-27" version="1">MCVCVCVCVCVYTCVVCVCVCVCVYITCVCACVYNPCLCVCVCVYIYLCLCVCVYIPVSVRVCVCVCVYIPVSVCVCITRVCVCVCVCIYTCVCVCVCIYLCLCVCVCVCVYTCVCACVYIPVLCVCVCVCVCVCVCIPVLCVCVCACVCIPVLCVCVCVCVYLCLCVCVYIPVLCVCMCVCIPVSVRVCVCIPVSVRVCVYTCVVCVCVCVCVYTFVCACVCIYLCCVCVCVCVCVYLCLCVCVYTCVVCVCVCVCVCVCIPVLCVCVCACVCIPVLCVCVCVCVCIPLSVRVCVYTCVCVCVCVYLCLCVCVYIPVVCVCVYLCLCVCVYIPVLCVCVCVCVCVCIPLSVRVCVYTCVVCVCVCVYLCLCVCVCVYLCLCVCVYIPVLCVCVCVCVYTFVCACVCIYLCCVCVCVCVRVCVCVCVYLCVCVCVCVCVCIPVSVRVCVYTCGVCVCVYLCLCVCVYIPVLCVCVCVCVCVCVCIPVLCVCARVCVYTCVVCVCACVCVCVCIPVLCVCVCVCVRVYLCCACACVCVYTCVVCVCVCVCVCVCIPVLCVCVCVCVCVCIPVLCVCVCVCVCVCVCVYTCVVCVCVCVCVCVCVCIPVLCVRVYLCCACACVCVYLCCVCVCVCVCVCVCVCVYLCLCVCVCIPVLCVCVCVCVCVCVCIPVLCVCVCVCVCVYTCVVCVCVCVCVCVCVYTCVVCVCVCVCVCVCVCVCVYLCCVCVCVCVCVCVCVCIPVLCVCVCVCVYTCVVCVCVCVCVCVCVYLCCVCVCVCVYTCVVCVCVCVCVCVYTCVVCVCVCVCVYTCVVCVCVCVCVYVQTAAFWLMARGVRDFVQNEGNGSLPVRGTIPDMIADSDKFISLQNVYREKALQDAAVVAKHIQSHLQAVGKPPESISEQDVKLFCKNAAFLRVVRCRSLAEEYSVDSFHKDTITSCMDSPDSEMVLYLMLRAVDRFYQQHSRYPGVYNYQVEEDISTLKLCVNSLLQEYSLNVNVKDDYVHEFCRYGAAEPHTVASFLGGAAAQEAIKLITHQFVPFNNTFIYNAMAQTSTTFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LNA0</accession>
    <name>A0AAR2LNA0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Plastin-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LCP1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LNA0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046965.1">
        <property type="protein sequence ID" value="ENSPNAP00000076147.1"/>
        <property type="gene ID" value="ENSPNAG00000019483.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183097"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21292">
        <property type="entry name" value="CH_PLS_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000059">
        <property type="entry name" value="Plastin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000012">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000014">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000025">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF35">
        <property type="entry name" value="PLASTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="9"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="118"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="262"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="392"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="513"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="71700" checksum="52222255B9DB8D38" modified="2024-11-27" version="1">MAKGEISAEEMEELREAFSKVDVDSNGYISTDELTELFKVANLPLPGYRVREIVQELSQTMDQNKDGKISFDEFVKVFHGLKSSEVAKTFRKAINKKEGICAVAGTSEQSGTQHSYSEEEKVAFVNWVNKALEKDPDCKHVLPMDPSSNDLFTAVGDGIILCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSSSAIGCHVVNIGAEDLKEGRQHLVLGLLWQIIKIGLLADIEISRNEALIALLRDGENLEDLMKLSPEELLLRWANYHLEQAGCSKINNFSSDIKDSKAYYNILNQVAPKGDEEGIPPIVIDLSGLREKDDLKRAECMLDQADRLGCRQFVMPPDVVRGNPKLNLAFVANLFNKYPALKKPENQDIDWSSIEGETREERTFRNWMNSLGVNPRVNHLYGDLDDALVIFQLYEKIKVPVDWDKVNKPPYPKLGSNMKKLENCNYAVELGKKEAKFSLVGIAGQDLNEGNRTLTLALLWQLMRRYTLNILEDLGDGQKVNDDTIVRWVNDTLTEAGKHTITAECISWTKMQTLILDLQDGSISSSMPVLDLIDAIQPGSIRYDLIKAEDLTDEEKLNNAKYAISMARKIGARVYALPEDLVEVKPKMVMTVFACLMARGMKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LP91</accession>
    <name>A0AAR2LP91_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SHROOM2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shroom family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000062214.1">
        <property type="protein sequence ID" value="ENSPNAP00000076554.1"/>
        <property type="gene ID" value="ENSPNAG00000025506.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155212"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06750">
        <property type="entry name" value="PDZ_shroom2_3_4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000100">
        <property type="entry name" value="Shroom family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014800">
        <property type="entry name" value="ASD1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014799">
        <property type="entry name" value="ASD2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027685">
        <property type="entry name" value="Shroom_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012">
        <property type="entry name" value="APICAL PROTEIN/SHROOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012:SF8">
        <property type="entry name" value="PROTEIN SHROOM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08688">
        <property type="entry name" value="ASD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08687">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51306">
        <property type="entry name" value="ASD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51307">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="12">
        <location>
            <begin position="53"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="ASD1" evidence="13">
        <location>
            <begin position="823"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="ASD2" evidence="14">
        <location>
            <begin position="1430"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="159"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="232"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="302"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="728"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="775"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="850"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="933"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1067"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1169"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1467"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1517"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1617"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="742"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="952"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="964"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1067"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1180"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1197"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1223"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1243"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1258"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1294"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1316"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1341"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1366"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1407"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1426"/>
            <end position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1733" mass="192477" checksum="2D87FE3E79C723A4" modified="2024-11-27" version="1">MYEVDTMGDHNLGSRLTDRDDPLIYDSSRTLDQNAGGEPGEGWRLVGVSLCGGAPWGFTLRGGREHGEPLIVTKVEEGSKAAAVSLQAGDELLNINDISLSGYRQEAICLVKGSHKTLTLVVKRRMKMVDIVAQKMPSESDVHVARSFLTKILRSSMRKNRFKGRNESMSRPHSWHSTKFNESQSETGKTQSTPSPVWQTRYDASSLSSDLTTGWDQTNLRRVSDQFSSLGSMESLEHGSHPYPPGRLSPSKSNANSIEHLGGGKRDSAYSSFSTSSGTPDYTLSKSNAASTENMLYKVSQWDSGSRPSNSRHSQNLNEGVQQDDRIGYLQLPSGTNDRESPKTVEQPGYRHSGSGKASTGPVWHIPDMKKTMAPSPPPPTPPTRRDSFAAIKVHEKGLVPSYPEGPGVHVQSKIHVKGVDRVSEVSDCTQKIYQASESALESCQNYNPPTKGDTVNSYKAAESYNQEAYHLNSNKTYSLSTTDVRAGHSSYIHLPHHQRQYSDESTFHAPSRMSSKPQNVSSCYSSMQELPTNSHNLHYSQNQVRRPVASLSSTTIDQNADIHSTTRYYCVTSRQHPPGLSQASLVRVEDWKANSVIDVSQSGHDRSAQGLQKDMYTPQVPLPLSNNKDINGYYRQVDSQHRTYPAVNTPFLDDAPAKASELCGIQKQSVVTNSDTHFISYPPKEESEQGRIAAPLQLRESSQEHLLSNSGADVCPQNTPLLHSLSQESSRLNENKDAMESGSSQQESVDPQTIRQVRRSDRFATTLRNEIQMRRAQLQKSRSAAALSGSLETEEEPSFWKPTENSSSSSDDSFTSSYKDHLKEAQARVLKATSFRRRDLEPVLLEHPGSEDLSSVWKDTPSMPSVSEVPLSKATSGGNQVTRICGRKRFPVEKKVRSLSEPDKIHEMGVDDEPVPLDNTVSLVGKRKFFEAKGKPAYQKPLPKQNQQIPDDPRLAKPEGKPQPSQSDTTTGDSNVKATAAENSPKEGQDGPNSLHRLGTFAEYEATWKTQRKVVEPRTSGRYHSADNILDQAVEQRNKPACFHERSRSSPSEDFYGQSILVQGRKSAEFPSSERKLSDHDNSGPSQNLSSSLSMEYGSWFFVPDRLNERGCNDLVSKNLEPPQSLSIGGDLERNLCSDPLPAHHKSLPNSACPPLYFPDHSILPETSSFTKTKGSISPRPPLPCKYPEATPPLPSSQGKATSHSPVRDTTSAPVPTQVFWKDPEYNKGPKSEEEQQSEITPPVPPSPAAPPIHQASGLTQPTMEGQRSPSPHFAPQRLTDKPPVSVSLQDEASGRMDRVTDDNTTVKKVPIKIVHSESNTEKESRQYLHPSSETPPSPQGSTVSSLSCLATPKQSCSPFCTYTRQRDQEGEVRDIMGNQKEQEPYADVTGPLEQHGEQKDNVNQSSNGVSSINLGSSSSPSEEDEKREVLARDIIDKDKSLADILDQSKMRTTMDLMEGIFPQEEQLMEEAHQRRRVAPKQASSRSSEDRREEDNMAAVSALVTSSAYYSTSAPKAELLIKMKDMQEQSVELDSEEELEEDNDSDLASKKHELIESLSKKLQVLREARESLQEDMQDNNALGEEVEATVQTVCKPNELEKFRMFVGDLDKVVSLLLSLSGRLARVENALNSLEEDASLEERRTLTEKRKLLIRQHEDAKELKENLDRRERVVYDILASCLQEESLADYKHFVKMKSALIIEQRKLEDKIKLGEEQLKCLMDSLPPDQRMPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LYR1</accession>
    <name>A0AAR2LYR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 14C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for normal heme biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LYR1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053475.1">
        <property type="protein sequence ID" value="ENSPNAP00000079461.1"/>
        <property type="gene ID" value="ENSPNAG00000025449.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154772"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070453">
        <property type="term" value="P:regulation of heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1740:FF:000002">
        <property type="entry name" value="Transmembrane protein 14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="146" mass="15328" checksum="E1F5A4D3173F9158" modified="2024-11-27" version="1">MAVDWAGYGYAALVASGGVLGYVKAGSVPSLAAGVLFGGLAGFGAYQISQNPKNIWVSLATSGTLAAIMGNRFYNSRKIMPAGIVAGARYLLHPPQQDSAPGHSEISELFLSFAGVIHTKLSYTISTTVVLTMILIITDVYCQIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M1X3</accession>
    <name>A0AAR2M1X3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M1X3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067907.1">
        <property type="protein sequence ID" value="ENSPNAP00000080984.1"/>
        <property type="gene ID" value="ENSPNAG00000032820.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000275812"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8973" checksum="096088417A68A0A7" modified="2024-11-27" version="1">RAGVSGIARAEGEHKVSLYADDMILYLSDASTSLPVVLNILSDFGKISGYRVNTQKSELMPINLAARESSFVYTLLYFLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M2C9</accession>
    <name>A0AAR2M2C9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M2C9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041579.1">
        <property type="protein sequence ID" value="ENSPNAP00000081144.1"/>
        <property type="gene ID" value="ENSPNAG00000003544.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286992"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF62">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="8"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="352" mass="40397" checksum="0568CDD38430FCF1" modified="2024-11-27" version="1">SNVCCSTKIELNIVLLGRSGDGKSSTKNTILGEKSAQERSSATSLVNQVKAKNLQRKTLRIVETPGLFDTSSTKEEFQQEVEKCITHCVPGLHAFIIILKVGRYTAQEARILDEIKELFGEDVLKYAVVLFTNGDQLEDGQTIEEFVQQGVALQELVDKCRGRVHVIDNKYWKQQQDGYRSNRVQVEKLLKTIEEMVKESRGQYYSKEMLQAVNKNKRSRRPFENVTQVVKKYSQGKGWAVVVLASVLVGFEKFQNVRGKKLNLHYKNLNNYFLIQSQSFPKTIFNSQLNQYLTHWLKHLKITKSHFSIISTSHSQPESQVIFKYFVFTSFDITARIHKKKMSHQRCVPAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRA9</accession>
    <name>A0A3B4BRA9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="14">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PYCR3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate (P5C) to L-proline using NAD(P)H. Proline is synthesized from either glutamate or ornithine; both are converted to P5C, and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCR3 is exclusively linked to the biosynthesis of proline from ornithine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:14107"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:14111"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodecamer; composed of 5 homodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017579236.1">
        <property type="nucleotide sequence ID" value="XM_017723747.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRA9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001125"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012703.2">
        <property type="protein sequence ID" value="ENSPNAP00000001125.1"/>
        <property type="gene ID" value="ENSPNAG00000000558.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443228"/>
    <dbReference type="CTD" id="65263"/>
    <dbReference type="GeneTree" id="ENSGT00950000183044"/>
    <dbReference type="OMA" id="AKQTCLG"/>
    <dbReference type="OrthoDB" id="10263291at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000367">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000003">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13 14" id="KW-0521">NADP</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 14" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="15">
        <location>
            <begin position="8"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="16">
        <location>
            <begin position="165"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="73"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="272" mass="28549" checksum="DA108C0BE6E0A854" modified="2018-12-05" version="1">MDELSQLKIGFIGAGNMAFGIARGILKSGDVPSSNVIISAPSRNNFPRFEDMGVAVTHSNKEVVDHSRLVFLAVKPHLIRTVLNGISEHVTRQHIIISVAAGITLATLEELLPVGSCVMRLMPNLPCMLQEGALLLACGKGAGQDVESLLKRLLSPCGLVEVGPETWIDVHTGLSGSGVAFVYLFAEALAEGAVKMGMPSELAQRIAAQTIIGAGQLLRDGGKHPAQLRAEVCTPGGTTIYGLHALEKGGLRAATMGAVEAATERARELGCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSN7</accession>
    <name>A0A3B4BSN7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HOXA13</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BSN7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002658"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010064.2">
        <property type="protein sequence ID" value="ENSPNAP00000002658.1"/>
        <property type="gene ID" value="ENSPNAG00000009029.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164071"/>
    <dbReference type="OMA" id="YCAKEQQ"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033333">
        <property type="term" value="P:fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000084">
        <property type="entry name" value="Homeobox protein Hox-D13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051003">
        <property type="entry name" value="AP_axis_regulatory_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022067">
        <property type="entry name" value="HoxA13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804:SF9">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A13A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804">
        <property type="entry name" value="SEGMENTATION PROTEIN FUSHI TARAZU-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12284">
        <property type="entry name" value="HoxA13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="211"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="213"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="279" mass="29860" checksum="64A03BF9CE238453" modified="2018-12-05" version="1">MTASVLLPSRWTEPVMFLYDGGSEDVGAGMEGFGAGGAGGGGGAGAGAVGGGGGAAAHFGYAPSEAPASSAAEAAKPPCPPPQPALPYGYFGGGYYPCRMAKPCAQPVTAFSADASVSGEAEFSSRAKELAFYPSYSAGHYQHVPGYLDMPVVPAEPRHEPILPVESYQPWAITNSWNSPVYCPKEQSQPGHLWKTSLQDSVSAADGGSARRGRKKRVPYTKVQLKELEREYAANKFITKDKRRRISAHTNLTERQVTIWFQNRRVKEKKVVNKFKSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSR3</accession>
    <name>A0A3B4BSR3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endothelin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">EDN1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor. Promotes mesenteric arterial wall remodeling via activation of ROCK signaling and subsequent colocalization of NFATC3 with F-actin filaments. NFATC3 then translocates to the nucleus where it subsequently promotes the transcription of the smooth muscle hypertrophy and differentiation marker ACTA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endothelin/sarafotoxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017549085.1">
        <property type="nucleotide sequence ID" value="XM_017693596.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BSR3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001560"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011935.2">
        <property type="protein sequence ID" value="ENSPNAP00000001560.1"/>
        <property type="gene ID" value="ENSPNAG00000008243.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425150"/>
    <dbReference type="CTD" id="1906"/>
    <dbReference type="GeneTree" id="ENSGT00950000183053"/>
    <dbReference type="OMA" id="EKWPARR"/>
    <dbReference type="OrthoDB" id="9362154at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031707">
        <property type="term" value="F:endothelin A receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031708">
        <property type="term" value="F:endothelin B receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003100">
        <property type="term" value="P:regulation of systemic arterial blood pressure by endothelin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014826">
        <property type="term" value="P:vein smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020475">
        <property type="entry name" value="Endothelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019764">
        <property type="entry name" value="Endothelin_toxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001928">
        <property type="entry name" value="Endothln-like_toxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13874">
        <property type="entry name" value="ENDOTHELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13874:SF10">
        <property type="entry name" value="ENDOTHELIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00322">
        <property type="entry name" value="Endothelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00365">
        <property type="entry name" value="ENDOTHELIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00272">
        <property type="entry name" value="END"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00270">
        <property type="entry name" value="ENDOTHELIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0838">Vasoactive</keyword>
    <keyword evidence="5" id="KW-0839">Vasoconstrictor</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017479604" description="Endothelin-1" evidence="8">
        <location>
            <begin position="23"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Endothelin-like toxin" evidence="9">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Endothelin-like toxin" evidence="9">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="194" mass="21633" checksum="FEE9B8E939656234" modified="2018-12-05" version="1">MELRIIFPVLSVLSSCVFHTAALPAWSLTEGAATPASARHSRTKRCSCATFLDKECVYFCHLDIIWVNTPERTVSYGLGNAPRRKRSAPRAASNDPARCRCVDSSDGACASFCHTDYPPRRKAAPDKVIPTACGHDCAKKQRKHDVAAQTRSIKRVRKSTVPSLATKQIRLLLEKWKLRRLHKTQAWAAENTMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTD6</accession>
    <name>A0A3B4BTD6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Contactin 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. Contactin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561984.1">
        <property type="nucleotide sequence ID" value="XM_017706495.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BTD6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001806"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011515.2">
        <property type="protein sequence ID" value="ENSPNAP00000001806.1"/>
        <property type="gene ID" value="ENSPNAG00000000947.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432562"/>
    <dbReference type="CTD" id="541474"/>
    <dbReference type="GeneTree" id="ENSGT00940000155915"/>
    <dbReference type="OMA" id="KNVQIWH"/>
    <dbReference type="OrthoDB" id="6138780at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000035">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000044">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000047">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000052">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000064">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF10">
        <property type="entry name" value="CONTACTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017291979" description="Contactin 1b" evidence="13">
        <location>
            <begin position="27"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="46"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="147"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="239"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="339"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="421"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="513"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="614"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="720"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="821"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1035" mass="117027" checksum="6B849376E0F7F1F7" modified="2018-12-05" version="1">MGGGITMAAPVVLLSILSSLLLPADAIKPRFFEPRIFDKEATGFGPIFEEEPLDTVYAEDSPEAKISMNCRVRANPPAAIKWWHNNWEIKLMEQPDEHYSLVGGNLVITNPEKSKHAGKYVCVAKNVYGTTISKEATVKFGYLDQFPTDEREAVYVKEGQGAVLLCAPPSRYPTEVMYRWIYNDFPNFIIPDQRRFVSQTTGNLYIAKVEASDVGNYSCFVSSPTIGKSVFSTPIPLVPQIEKEVKRYPADIRVRFPKTYALVGQNVTLECFALGNPLPHIRWRKLDADLPPNYEVSMNGALLHLINVQYEDEGSYECEALNVRGMDWYRQWLYVEGAPEWAEHINDTEKDVGSELTLSCTAVGKPLPWIRWLKDGYSYGKGELKFSSLTFEDSGMYQCIAENTWGTIYANAELRVVSCAPTFIYNPVKKTLLGAENGRVVIECKPRAAPKPRFIWKRGSELFSNSSRMFIWDDGSLEILNATKSDEGLYTCYAENDRGKANSTGTLSITEATKITVPPSDVEVTVGETTVLQCSASYDPSLDITFIWTVDSYIINFATDFDHYEQMMGRENSGDLLIKNIQIAHAGEYICTAQTIVDNTTASANVIVRGRPGPPGGVRVEEIRDTSVKLVWSLGSDHGSLLAQHVVQTRDFFALDPEDWKTAVTSPVFLDGKAEVTEVVDLYPWMEYEFRVYAINELGAGESSLPSIKIKTWDAIPTMSPTDVDGFVGLNGELIVTWTPMKPQYFFGKKFGYVVAFKPHEDFDWKWSTIADPEARRFVYRDTIIPNTEIQVKVKGYNSKGEGPYSLTKVVFSSESAPTEAPLDVYARQVTSTEALVWWLPVYQVPPHWVDGYQIRYWRKYDDNEAAASRVLVHSSVNQTRLENMRPDSHYLIEVRAYNGAGLGPPSEHCEMFTRRPPPSRRLRVYKYVSFTRKWLYIYWDHIYNYWNESYVEGYKILFRKEGERYGKLYTTGRHYIDFPMPETGDYVIEIRARCEGGDGPISQILVQGRAALGSQSLGAVSLLLLVLAWMNLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BV22</accession>
    <name>A0A3B4BV22_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small glutamine-rich tetratricopeptide repeat-containing protein beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-SGT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SGTB</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homooligomerize.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SGT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BV22"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003492"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008622.2">
        <property type="protein sequence ID" value="ENSPNAP00000003492.2"/>
        <property type="gene ID" value="ENSPNAG00000009953.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444357"/>
    <dbReference type="CTD" id="54557"/>
    <dbReference type="GeneTree" id="ENSGT00940000158321"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.420:FF:000002">
        <property type="entry name" value="Small glutamine-rich tetratricopeptide repeat-containing protein alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000103">
        <property type="entry name" value="small glutamine-rich tetratricopeptide repeat-containing protein beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032374">
        <property type="entry name" value="SGTA_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF1">
        <property type="entry name" value="SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE REPEAT-CONTAINING PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16546">
        <property type="entry name" value="SGTA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="SGTA homodimerisation" evidence="12">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="121"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="155"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003492.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="306" mass="33694" checksum="823FE4A1AD80B1FE" modified="2024-11-27" version="2">MSVEKRLAYSIVQFLRDQTHCGSLNSDEQESLEVAIQCLETTFKISSSDCNLEAPQPLREMFLNSLLKNDIVSLQETSPSPEDIERAEQLKNEGNNHMKEENYSCAVECYTKAIHLDQRNAVYYCNRAAAHSKLGNYTEATGDCERAIAIDPTYSKAYGRMGLALTSMSKYPEAITYFKKALVLDPENETYKSNLKVAEQKNKEASSSTAAGLGFDMASLINNPAFISMAASVMQNQQVQQLMSGMMSNAVGGPAAGVGGMSDISSLIEAGQQFAQQIQQQNPELIEQLRNHIRSRSFSGSAEEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVQ7</accession>
    <name>A0A3B4BVQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ETS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ERF</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563992.1">
        <property type="nucleotide sequence ID" value="XM_017708503.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVQ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003713"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008246.2">
        <property type="protein sequence ID" value="ENSPNAP00000003713.1"/>
        <property type="gene ID" value="ENSPNAG00000010147.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433745"/>
    <dbReference type="CTD" id="2077"/>
    <dbReference type="GeneTree" id="ENSGT00940000157292"/>
    <dbReference type="OMA" id="THHLHAP"/>
    <dbReference type="OrthoDB" id="8964711at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903708">
        <property type="term" value="P:positive regulation of hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000059">
        <property type="entry name" value="ETS translocation variant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF313">
        <property type="entry name" value="ETS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="9">
        <location>
            <begin position="27"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="228"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="334"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="542"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="397"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="542"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="617" mass="66907" checksum="8F179531E848F2FF" modified="2018-12-05" version="1">MKTPGDSGFAFPEWAYKPESSPGSRQIQLWHFILELLRKEEYHDVIAWQGDYGEFVIKDPDEVARLWGVRKCKPQMNYDKLSRALRYYYNKRILHKTKGKRFTYKFNFNKLVLVNYPFIDMGSAGSGVPQSAPPVPSGVGTHFRFPPSTPSDVLSPSEELRSPGVFSAVARRMARGSVSDCSDGTSTNSELEEAGVAAGDDRPADRVFRNLLHPRLSHDSLFRVYGAPPNPPGLSRGGPHAPPHRVHPEPLSPFPVSPLPGPGGLLAPPLSPALSMTPTSHLPYTPSPSLSPMLGSHFSFNPEDMKRYLQAHTQSVYNYHLSPRAFLHYPNIVIPQPQRPDKGLGGSAAAGPHPPTHPLHHHQSEEPHLSPFKFKLQPPPLGRKQREGLPASGAGHTPSITTSGLGSSSSQCSNSASSGLVNSSGGPPKIKVEPISDVESEEEVEVTDISEEDDNCDVFAPPHSNGGPAPLPCNDGIVDDDDLEEDVFKTPAAPTSGAGPGLGLALLGLKAEPRELGLGQGPPSSPGGTRCIPLKLRFKRRWSEDQRMEAEAEEAEDKKSRAEKEEPVQEAEPKRVEENRDGKSPVSLAAPLGTGQRRVSSELQRATAQLSLENNGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVY0</accession>
    <name>A0A3B4BVY0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ZFAND1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVY0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008092.2">
        <property type="protein sequence ID" value="ENSPNAP00000003807.2"/>
        <property type="gene ID" value="ENSPNAG00000010254.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111180"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035617">
        <property type="term" value="P:stress granule disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057358">
        <property type="entry name" value="UBL_ZFAND1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF37">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25327">
        <property type="entry name" value="UBL_ZFAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="5">
        <location>
            <begin position="18"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="5">
        <location>
            <begin position="72"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003807.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="284" mass="31125" checksum="F7FC1F45BCE689A8" modified="2024-11-27" version="2">MTFILKAFSCAELSMAELDIGKHCETADCGQKDFLPFVCDSCSGVFCLEHRSRDSHACPEVPVRKEVCGSGSRTSYACTFENCKGRELLPVICPQCQKHFCLAHRHQDDHKCEKLETPKPRMVATQELVQKIVDSKKSVLMSKGRKGAKNSATAAKVALMKLKLHASGDKGLPQTERTYFQVFLPKEAAASSQPMFFSSKWSVGKVVDFAASQASLKNNNNVLTARKLRLCHPETGEALKMDASLQSLLSLPDSPLFNGGNVILEYLDNESAGVDDVTAYIPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BW24</accession>
    <name>A0A3B4BW24_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017573065.1">
        <property type="nucleotide sequence ID" value="XM_017717576.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BW24"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003162"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008041.2">
        <property type="protein sequence ID" value="ENSPNAP00000003818.1"/>
        <property type="gene ID" value="ENSPNAG00000010249.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439279"/>
    <dbReference type="CTD" id="564537"/>
    <dbReference type="GeneTree" id="ENSGT00940000159004"/>
    <dbReference type="OrthoDB" id="5914531at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09945">
        <property type="entry name" value="SH2_SHB_SHD_SHE_SHF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000021">
        <property type="entry name" value="Putative SH2 domain-containing adapter protein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051846">
        <property type="entry name" value="SH2_domain_adapters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127">
        <property type="entry name" value="HEAVYWEIGHT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127:SF33">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING ADAPTER PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0727">SH2 domain</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="107"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="244"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="415" mass="46827" checksum="F2D0693DE91A4EFD" modified="2018-12-05" version="1">MAKWLKDYLSFGSKRMPPQPPKPDYTESEILKAYRVQKSLDFEDPYEDYENKGRNDSGSAESPLAGIGSMLKNSALDVKVVSPKHRLIKVDSQDLGRTKILLSSVSLEDPTEPVVPSAPAIGDTDYSDPFDARQDLQPESRQTPTTPENNGYMEPYEAQRVITELQRRAGGRGRGEVQLYDTPYEEQGSGPMEPTEEGREGRLPQDDERPADEYDQPWEWKKDHISKAFAGSDLKELAELPWPPPVGQLEEEPSLGEQVQFEGADWERSSPPPDRLRSPRAPLVTGNMKLRKPSESHPMLGERVDPTLPLEKQVWYHGALSRSDAESLLTLCKECSYLVRNSETSHSEYSLSLRSCQGFMHMKFSQSKDGKYILGQNSPPFSNIPEVIHYYTTHKLPIRGAEHLSLLFPVLVQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWM8</accession>
    <name>A0A3B4BWM8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Regulator of nonsense transcripts 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Up-frameshift suppressor 2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">UPF2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWM8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009609.2">
        <property type="protein sequence ID" value="ENSPNAP00000002920.2"/>
        <property type="gene ID" value="ENSPNAG00000009357.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064318"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000014">
        <property type="entry name" value="Putative regulator of nonsense transcripts 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.160:FF:000002">
        <property type="entry name" value="Putative regulator of nonsense transcripts 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000015">
        <property type="entry name" value="regulator of nonsense transcripts 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000023">
        <property type="entry name" value="regulator of nonsense transcripts 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="MIF4G" evidence="12">
        <location>
            <begin position="171"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="12">
        <location>
            <begin position="585"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="12">
        <location>
            <begin position="789"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="552"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1036"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1246"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1045"/>
            <end position="1095"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002920.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1318" mass="152791" checksum="8ACDE8BC51F66AF0" modified="2024-11-27" version="2">MPAERKKSVNMDEKETSSFSNKEKERDREGERRPTSSREKGKEEGKMSGKKDGIKSADDKKRRVEEEKRKKEEKERKKKEEEKQRAEEEQKRKEEEEKRQQEEQEKKLLEEEAKRQKEEAAAQLKEKEEAQQLHQEAWERHQSRKELRGKNQHAQESRPEEAFFSRLDSSLKKNTAFVKKLRTLTEQQRDSLSHDFASLNLSKYIGEAVSSLVEAKLKISDVGCAVHLCSLFHQRYADFAPLLLAAWKRHFEARKEEKSPNVSKLRTDLRFIAELTIVGLFTDKEGLSLIYEQLKNIIGADRETHTHVSVVISFCKHCGDDIAGLVPRRVKSAAEKFNLTFPPSEIINTEKQQPFQNLLREYFTSLTKHLKKDHRELQNIERQNRRILHSKGELSEDRHKQYEEFATCYQKLLANTQSLADLLDENMPDLPQDKTVQEEHGPGIDIFTPGKPGDYELEGGIWEDEDARNFYENLVDLKAFVPAILFKDNEKASQSKDKDENKEIKEGREGKETSSTEELELELEALDITDDPLELEVADEAESEELAKKLLDEQGKYSRPHSHQTASEQEDEEASTGSHLKLIVDAFIQQLPNCVNRDLIDKAAMDFCMNMNTKSNRRKLVRALFTVPRQRLDLLPFYSRLVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELAKFKMFSKTDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAQHLDARYVTMVENAYYYCNPPPMEKTVRKKRPPLQEYIRKLLYKDLSKVTTEKVLRQMRKLPWQDPDMKSYLICCMVNIWNVKYNSIHCVANLLAGLVAYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLFSFISFGVSTDGTPSPLDPPEHLFRIRLVCTLLDTCGQYFDRGSSKRKLDCFLIYFQRYIWWKKSLEVWTKDHPFPIDIDYMISDMLELLRPKMHLCSSLEDACRHVTELEREVLVKLGLAMEKDGRCSSAMGEGEALDEEDEDDDDEGGAETEEQSGNESEMNEPEEEEGSENEEEEREEEEENTDYLTDSNKENETDEENNEVMIRGGGLKHVACAEDDDFIQALDKMMLENLQQRSGEAVKVHQLDVAIPLHLKSQLKKGPAPSCTAETDTDINDTMQFVMLTRKGNKQQFKILNVPLSSHLAANHFNQQQAEQEERMRMKKLTLDINERQEQEDYQEMMQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGREESRASQRRSRVTKIGGREATQLGKQPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BX11</accession>
    <name>A0A3B4BX11_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017557368.1">
        <property type="nucleotide sequence ID" value="XM_017701879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BX11"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004222"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007359.2">
        <property type="protein sequence ID" value="ENSPNAP00000004222.1"/>
        <property type="gene ID" value="ENSPNAG00000010674.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429839"/>
    <dbReference type="CTD" id="403183"/>
    <dbReference type="GeneTree" id="ENSGT00940000159229"/>
    <dbReference type="OrthoDB" id="413520at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF1">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 21E PSEUDOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="243" mass="27949" checksum="43D794592D5EC0B0" modified="2018-12-05" version="1">MERDPAEESEAKDEAPDAELATAIMKRHFRPSLIKTEAWEGYTFADLEIKIKESTDCYGAVLWPSAMVLCHFLDTHREKYNLVDKNVIEIGAGTGLVTIVCSLLGAKVTSTDLPDVLSNLRYNVSRNTRGHCRYETQVMELSWGQQLEERFPHASCHYDYIMAADVVYAHPYLDELMETFEHLCSQDTVILWAMRFRLEPENSFVDRFQTRFHLEELYDLPSLRIKLYRAQKKVSQSQSGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2G8</accession>
    <name>A0A3B4C2G8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2G8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005031"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006000.2">
        <property type="protein sequence ID" value="ENSPNAP00000005031.2"/>
        <property type="gene ID" value="ENSPNAG00000011369.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163793"/>
    <dbReference type="OMA" id="QLYRWIH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="126"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005031.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="275" mass="32309" checksum="A3211937AA5E6570" modified="2024-11-27" version="2">MWNVSKVTLQLSTTRASDRLFQPLWDYLVFHHYSLVSSPFFPLMLTFSSYFLFSCPFAILDLLGEQSPAFYGYMMLVYWSEILQGYKCSLRCYFYICYLVSPQCFVQFLLDYNLLCLVVGALLWGAWCLDTQYFFWHVAHHKNSHLYKWVYAIHHYYISPFFWSIEHLSAVEVMTVGFWSNLDPLLLQCHPLTTWTLTVFSIWLSVEDHIGYDLPWTPSRLVPFGLLGGALAHDLHHQKPSSNFAPFFSHWDRLFGTSNNVVKKVFMNKTASKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2X2</accession>
    <name>A0A3B4C2X2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PER3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017576429.1">
        <property type="nucleotide sequence ID" value="XM_017720940.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2X2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006113"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004118.2">
        <property type="protein sequence ID" value="ENSPNAP00000006113.1"/>
        <property type="gene ID" value="ENSPNAG00000012241.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441424"/>
    <dbReference type="CTD" id="8863"/>
    <dbReference type="GeneTree" id="ENSGT00940000160817"/>
    <dbReference type="OMA" id="GISQCSY"/>
    <dbReference type="OrthoDB" id="7788983at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000013">
        <property type="entry name" value="Period circadian protein homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000004">
        <property type="entry name" value="Period circadian protein homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057310">
        <property type="entry name" value="PER1-3_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048814">
        <property type="entry name" value="Per1-3_PAS-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022728">
        <property type="entry name" value="Period_circadian-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050760">
        <property type="entry name" value="Period_circadian_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269:SF13">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23170">
        <property type="entry name" value="bHLH_PER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21353">
        <property type="entry name" value="Per3-like_PAS-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12114">
        <property type="entry name" value="Period_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="10">
        <location>
            <begin position="400"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="607"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="672"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="722"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="798"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="942"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1007"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1056"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1209"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="116"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="737"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="798"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="841"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1018"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1033"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1066"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1084"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1107"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1220"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1233"/>
            <end position="1246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1246" mass="135150" checksum="EE933CA1D87FA00F" modified="2018-12-05" version="1">MPSGDSCEDGEENVSPSPRRGAQVDQTSSEPELGPSGSASSAGPLSGSGSGEDEEAEERPRQPGIGESSVTCEESGGEPSGGEQGQEDVEMNSGHDSSSGNDSMGRSRRHSPSANRSPGSSTGSASAKSSKSATGSSSSSIRSAASGQRHHTGCNEQTEQERGHAHREMMQTVQEMKKRLPSEKRSRSKASTVEALNYALKCVKQVQANSEYYKLLMCNGLEERRDATVCTQEQLEGITSEHTLKNTDTFVIVFSLATGRVIYASEQASSILNCKRKFLDSAKFVELLFHQDVNVFYSHTAQPHLPPWNVDTDSAAVLFESAQVKSFFCRIRGGKDRDGEMRYNPFRITPYLLKVRSVGGEEEQPCCLALVERIISGYEAPRIPTDKRIFTTTHSPGCVFLEVDDRAVPLLGYLPQDLIGTSLLTCIHPEDRMLMLTMHRKVLKYAGQPPFEHSPVRFRSQNGDYVTLDSSWSSFVNPWSRKVAFIIGRHKVRTGPLNEDVFAAQNKVEPLVMHEDVKELQAIIYKLFLQPVHNNGSSGYGSLGSNGSHEHYISVASSSDSNGNLWEDSHREPVTLQQICANVNKVKRGGQQAYLDSRRKLRPVGKVTTETQAPLPRGGQVQSLQASLEESHRKQTHIPSYQQINCVDSIIRYLESCSGPALKRKSETLSIAASSSSSTSEEDKPPPAPLEDVANRDGPTLKAMSVVSSQCSYSSTIVHVPQPESEVTALEDAPMGSESADPAPAPARPALSSSSAAQEEFHIVGLTKEVLSAHTQKEEQEYVDRFRHRILESPFSSYLQQDNGGWNTSHSGKPKHKRPKPQCSSDSDVSRPGMCPPLCSPAPNNSSSESSKPNFISPMLMPVQPPYYPMAAPPGAEQAPSTVPMVLQPEQMQSMQPLVQVMNPFPIVAVILPYPANYVYPQGIPGIQGIGPHPSFHQPPCMSMVDTSPQAGPSGMPPVPNTLLDSPILSPWQGEDLDGAQPTALFSSSRSSSPLQLNLLQEELPKLTEPQSSTGRPESLHEQHAKGVDAPSESGNQDAQSTSSVMLDLLLHEDARSGTGSNASGSGSGESGGSLGSGSGLGSNGTSTSHTGSSNSSKYFASNDSSDTSRKARKSQEAQERTTYEKCVESSLWTMIQHTPEPVMMTYQIHPRDQAQVLKEDREKLLLLQPMQPWFSQEQKEELAEVHSWIHRRTVPEEINTQGCVSCNTAGLEGGEVAADSPDLTTPDSSSPQNCPTEDITPTSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4E1</accession>
    <name>A0A3B4C4E1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class II family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578092.2">
        <property type="nucleotide sequence ID" value="XM_017722603.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4E1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004796.2">
        <property type="protein sequence ID" value="ENSPNAP00000005726.2"/>
        <property type="gene ID" value="ENSPNAG00000012437.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442524"/>
    <dbReference type="GeneTree" id="ENSGT00940000161847"/>
    <dbReference type="OMA" id="ISESPEW"/>
    <dbReference type="OrthoDB" id="8925804at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002504">
        <property type="term" value="P:antigen processing and presentation of peptide or polysaccharide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001003">
        <property type="entry name" value="MHC_II_a_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF86">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DR ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00993">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00920">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0491">MHC II</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043725021" description="Ig-like domain-containing protein" evidence="14">
        <location>
            <begin position="20"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="96"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005726.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="233" mass="26171" checksum="3B78B1C7C18F0D68" modified="2024-11-27" version="2">MKPCLILIWAAFLSTEAKTQHRVVGLILCSDTEREVMYGDNEEEMYHIDFSKMKGVMTLPRFSEPISYPIAYESSMGRMEVCKSSLKIWTKNYSNPEPLDAPQSSIYSKDELEVGFENTLICYITGFYPPHVKVSWTKNNVNVTDEVSLSRYYPTKDGSFNVVSFLKFIPEQGDIYTCTVEHAALDSPLTRTWDVEVTLPNVGLSIFCGVGLAAGMMGVAVGTFFFIKGNNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4S0</accession>
    <name>A0A3B4C4S0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dystrobrevin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">DTNA</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the dystrophin family. Dystrobrevin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4S0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006055"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004226.2">
        <property type="protein sequence ID" value="ENSPNAP00000006055.1"/>
        <property type="gene ID" value="ENSPNAG00000002134.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153897"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16249">
        <property type="entry name" value="EFh_DTNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000014">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000016">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000002">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017432">
        <property type="entry name" value="Distrobrevin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF19">
        <property type="entry name" value="DYSTROBREVIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038204">
        <property type="entry name" value="Distrobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="17">
        <location>
            <begin position="242"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="553"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="559"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="575"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="716" mass="80567" checksum="A14541862CD4DD8F" modified="2018-12-05" version="1">MVLSEGMIEDHSSRTMADRRQLFAEMRALELDSIRLSTYRTACKLRFVQKKCNLYLVDIWNLIEAFRENGLNTMDLNTEFSVARLEAILSTIFYQLNKRMPTTHQINVEQSILVLLNFLLAAYDPEGQGKISAFVVKMALGTLCGGKISDKLRYIFSQIADPKGIMIYSQFDQFLKEVLKLPVTVFEGPSFGYTEQTTRTCFPQQKQISLNMFLDAFMSDPPAQCLVWLPLMHRLANVENVFHPVECSYCHSQSMMGFRYRCQQCHNYQLCQECFWKGHASGSHSNQHQMKEYTSWKSPAKKLSNALSKSLSCASNRETLRPIYPDAPEKPLNLAHVVPPRPVNSGNEYMLCHSMPSTSNPYSSRVGANDDVKQRKLLARAAPDLLKGKGVQYSFDIADRLADEHVLIGLYVNLLQKSRSCLLESSDSNQDDEHCLIARYASRLAADGTSVQPRAPGDISVSLDTNKQQRQLIAELESKNKEILQEIQRLKQQHDEASQPPADKGQQNPTLLAELRQLRQRKEELEQRMSALQESRRELMVQLEQLMLLLKTQGPAPSPRSSPSHTLSSSIPMPVQSTSGNTTPSQTPQDSLMGLGGDVQQAFAQSSRRNLRNDLLVAADSITSTMSSLVKELHSEAGSESDSNVDTEAERSQFDDFTSAAAESLSAQLNHSKARMMREEGYKNTLEQRLERELKMEEQTAAGESLHGDFATVALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C847</accession>
    <name>A0A3B4C847_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Ribophorin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RPN2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Interacts with DDI2. Interacts with TMEM35A/NACHO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Endoplasmic reticulum membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the SWP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572039.1">
        <property type="nucleotide sequence ID" value="XM_017716550.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037388429.1">
        <property type="nucleotide sequence ID" value="XM_037532532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C847"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007843"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001157.2">
        <property type="protein sequence ID" value="ENSPNAP00000007843.1"/>
        <property type="gene ID" value="ENSPNAG00000013579.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438620"/>
    <dbReference type="CTD" id="6185"/>
    <dbReference type="GeneTree" id="ENSGT00390000002635"/>
    <dbReference type="OrthoDB" id="432292at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055375">
        <property type="entry name" value="Ribophorin_II_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055374">
        <property type="entry name" value="Ribophorin_II_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055373">
        <property type="entry name" value="Ribophorin_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05817">
        <property type="entry name" value="Ribophorin_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23861">
        <property type="entry name" value="Ribophorin_II_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23860">
        <property type="entry name" value="Ribophorin_II_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5019615582" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2" evidence="12">
        <location>
            <begin position="21"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="600"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II N-terminal" evidence="13">
        <location>
            <begin position="29"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II second" evidence="15">
        <location>
            <begin position="269"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II third" evidence="14">
        <location>
            <begin position="375"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="16">
        <location>
            <begin position="528"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="642" mass="69926" checksum="1678133B77A1329E" modified="2018-12-05" version="1">MALLRLVSLLVFVLALEAQALTPSHYLVASDVDRLQAVLSQPFTDLKSAYFSVVGLSKLGVSVADSNDACSFIKANVDSSSIEALFYAAEASQAISSCEIHVSNETRDSLHAAVSEDSTVSQIHQAVSALSSLGLPLASQEVVSALKARISKEDNVVAITLALQTASRLSQQAELGEILEEIEDLAARLDDLGGVYLQFEEGLEATALFVSAAYALSDHVDMEPPLKEDQVIQLVNSIFSKKSWDSLSEAFSVASAAAALSNNRFHKPVVVSAVGPATVSHSHPLLQLHVTDVLCQPLSSANVLVESATSVASKAAILSQTPFTLRDGIFELNFMASKPASGYYQFSVAVTGDSRLVANHVELKVKVSTKVAINNMDLSVVDKDQSIGTKTTRVEYPNKGKASFIADSHQNFAMTFQLVDETTGVELTPHQTFVRLHNQKTGQEVVFVAEPDSKNLYKFELDTAERKTEFDSISGTYALYLMVGDATLENPVLWNVADVVLKFPDEEAPSTVQSKNLYVPKPEIQHLFREPEKRPPTVLSNTFTALVLSPFLLLLILWFKLGANISNFSFSPSSVLFHVGHAAMLGLMYVYWTHLNMFQTLKYLAIIGSLTFLAGNRMLAQKAVKRIAAEQSSRLAKYRSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8K3</accession>
    <name>A0A3B4C8K3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8K3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006769"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002978.2">
        <property type="protein sequence ID" value="ENSPNAP00000006769.2"/>
        <property type="gene ID" value="ENSPNAG00000037508.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171455"/>
    <dbReference type="OMA" id="AHIDKTE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043893231" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="23"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006769.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="152" mass="16648" checksum="812C76901D4AF251" modified="2024-11-27" version="2">MMNLTSEILLNILFSFLTGVSSEDLITSHGSVKHARDGESVTLSCNYSVSQYGLFWYHQYSRSKPEFLLSTTESGGTVKADPAAQFSATVDKIQNSKVLNLVISSAAVSDSALYYCALQPTVTGNPATLYKNTVWNGALFLLMFLCFCTVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8X7</accession>
    <name>A0A3B4C8X7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IRG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538197.1">
        <property type="nucleotide sequence ID" value="XM_017682708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8X7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002766.2">
        <property type="protein sequence ID" value="ENSPNAP00000006884.1"/>
        <property type="gene ID" value="ENSPNAG00000012840.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411237"/>
    <dbReference type="GeneTree" id="ENSGT00950000183007"/>
    <dbReference type="OMA" id="WPTDEYV"/>
    <dbReference type="OrthoDB" id="422720at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
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        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
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        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF10">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="6">
        <location>
            <begin position="37"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="383" mass="42266" checksum="D3E1DCBA64E547C4" modified="2018-12-05" version="1">MASQNPEINEVIQASGESTLEGATRRAEKQVDELVHVSLSVAVTGETGAGKSTFINVIRGLDDGDNDAAETGVTETTTEPTSYKHPSMENVEFWDLPGIGSPNFKAKNYLKQVQFDRYDFFIIISSERFRENDIKLAKKIQKRKKLFYFVRSKIDNDIRDEAQREQFNEEETLSKIRRNCEENLKGIENPKVFLISSMNLSKFDFGKLVATLMTELPELKRQALLQSVPVSSLALLEEKVRMFKKAVWAVALLSGGVAAAPVPGLSFACDTSILLSFFTRCYYSFGLDDKSLDKLSKRVNKPHLKSLITSPLVLGLASKSAVRLQLSALAGSALVKNLCKLVPGVGNAAAGVISFTLTFTLLKRGLKDLADSARTMLREAGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CCR1</accession>
    <name>A0A3B4CCR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Nuclear factor of activated T-cells 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">T-cell transcription factor NFAT5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer when bound to DNA, completely encircles its DNA target. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm. Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017562780.1">
        <property type="nucleotide sequence ID" value="XM_017707291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CCR1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008239"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000478.2">
        <property type="protein sequence ID" value="ENSPNAP00000008239.1"/>
        <property type="gene ID" value="ENSPNAG00000002964.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433040"/>
    <dbReference type="CTD" id="555802"/>
    <dbReference type="GeneTree" id="ENSGT00940000155213"/>
    <dbReference type="OMA" id="PYQNQVI"/>
    <dbReference type="OrthoDB" id="5346094at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01178">
        <property type="entry name" value="IPT_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07882">
        <property type="entry name" value="RHD-n_TonEBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000174">
        <property type="entry name" value="Nuclear factor of activated T-cells 5, tonicity-responsive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000002">
        <property type="entry name" value="Nuclear factor of activated T-cells 5, tonicity-responsive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015646">
        <property type="entry name" value="NFAT5_RHD_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF10">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RHD" evidence="23">
        <location>
            <begin position="291"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="39"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
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            <begin position="207"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="268"/>
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        </location>
    </feature>
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        <location>
            <begin position="641"/>
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        </location>
    </feature>
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        <location>
            <begin position="703"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="978"/>
            <end position="1014"/>
        </location>
    </feature>
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        <location>
            <begin position="1036"/>
            <end position="1102"/>
        </location>
    </feature>
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        <location>
            <begin position="765"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="22">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="653"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="703"/>
            <end position="715"/>
        </location>
    </feature>
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        <location>
            <begin position="978"/>
            <end position="996"/>
        </location>
    </feature>
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        <location>
            <begin position="997"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1036"/>
            <end position="1046"/>
        </location>
    </feature>
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        <location>
            <begin position="1047"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1082"/>
            <end position="1102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1268" mass="137239" checksum="3F6E007D8320D1DD" modified="2018-12-05" version="1">MPSDFSSLFSADLDLRSPNSLYSKESVYDLLPKELQLPPCVEENSATMSQKSGGETGSPPATVLSSDASSISSGMTMGSPSSAFPTSSSFGMHSSTSATDRGPVHENSTPGEVGSRRKAEMLAAEGKLPSGSGGSGRGCGEKGGGASQQQHHHQMMPSKRPVLNISPPPEDLFDDSHMSGCQDDLLQDSEQSNSIWTENSVSNFSLMSSSSYNDNTEVPRKARKRTPRQRPGSKPASAEETNMDVFDADSAKGPHFVLSQLGPDSKACVKTSSNGNQASGQKGGILSGQYPQKSEGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTIKLEGMKEPVVLQVFVGNDAGRVKPHGFYQACRVTGRNTTACREVDIEGTTVIEVSLDPSNNMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLVFRVNIARADGSVLTLQTPSSPILCTQPAGVPEILKKSLHSCSVQGGEEVFIIGKNFLKGTKVIFQENVSDEKSWRAEAEIDMELFHQNHLIVKVPPYQNQAITCPVSVGIYVLTNAGRSHDVQPFTYTPDPATDVSVKKETLPPVKPCSFEQIKVMDGGLMVPILPLVKSEEVTPMEVSSNTGNFKQTSDLMNSAQQTLEINNNISTNSQSFSSSLACQPAEQEQPQSSVFSSKEPLSTIQKQDIAPNSSFHVPGKPLLQQGTQQFLLDTESLTQERSGSSTGTMVGLSQMDDSPQHQLSLLPPDEVAQLEQAVRQLQAKGFCNIHLQSEDSLAKKQQHRLQQHQQMQQQIQQQQQQQQQQMQQQQVLENLQHQLFHPQVQMQCSPEQQGSSQSIVENNNALFQQPSAQQQQAALFQQAKDLISIQTSYLQQTPSHSSPPLFHTSTSLAEAQAPQEAMFHTQKTSPTQEQVQASLFQNTMNVLDGSSLSAEPQPSATSMFLSQSALPGQLAVNNNQSQQLAFLSSMQTSALEAQSVFQASAQLAPIQQSTPMDQQSPQQTSQTQPGSLFQGISQSSNAKLSPNQQQAGLLFNSLSSAVAPEQNSGLLFSGQPQMTSMNSSSLISSEQQNASRPFAQTSLVTVRQEEASEPMSFQDQSTDVANQSASKQTMFQEQQPMQLAPSSGTSSGNLFVAQPNMPRSIASQEALFAPQNGVAGLQTTTSSPVQQPGSLFQTTVSGSLNQPSQNQQPSIFLFEIHNDCTQLMNSQGTTLSDQIIAISQSGQNQRESDGHIHSLLNQSMSESGNIQNVLTTSQNMEKIDDLLVSLQEQGKNHPHSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CCW2</accession>
    <name>A0A3B4CCW2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">VAT1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541592.1">
        <property type="nucleotide sequence ID" value="XM_017686103.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CCW2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008294"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000379.2">
        <property type="protein sequence ID" value="ENSPNAP00000008294.1"/>
        <property type="gene ID" value="ENSPNAG00000013902.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413532"/>
    <dbReference type="CTD" id="10493"/>
    <dbReference type="GeneTree" id="ENSGT00940000157579"/>
    <dbReference type="OMA" id="VWMPYLT"/>
    <dbReference type="OrthoDB" id="203908at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010637">
        <property type="term" value="P:negative regulation of mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08275">
        <property type="entry name" value="MDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052100">
        <property type="entry name" value="SV-ATPase_mito-regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44054:SF1">
        <property type="entry name" value="SYNAPTIC VESICLE MEMBRANE PROTEIN VAT-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44054">
        <property type="entry name" value="SYNAPTIC VESICLE MEMBRANE PROTEIN VAT-1 HOMOLOG-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="80"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="404"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="411"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="480" mass="53090" checksum="93275BA10F6D7576" modified="2018-12-05" version="1">MSGEDAPSQQQNADEQKQQQQPQENTKPAESPKTENKPEPPPAEAAAAAAATATAAAAPAEKPAEEDAFSYRALVLTGYGGYDKVKLQVKKGKPALKAGEVMVRVRACGLNFADLMARQGLYDRLPSPPVTPGMECSGIIEAVGEEVTDRKVGDKVMVLNRSGLWQEVVVVAATHTFLMPEGMSFEEAAALPVNYITAYMMLFDFGHLRPNQSVLIHMAAGGVGIAATQLCKTVKDVTVFGTASAAKHEVISEGGVTHPIDYRTRDYVEEVRKISPKGLDIVLDPLGGSDTHKGYNLLKPMGKLISYGAANMLSGQKKNLFAVAKTWYQQFSVHTLSLIQGNRSVCGFHLGYLDTETELLTMAMNAVLDLYHQGKVKPRIDSTYHLEQVGDAMRRMQERNNIGKVILITEPMKEEPKKEEAKKDEKEKKEDKKKEDKKKEDKKKEEAKKEEKKEEKKKEEAKKEDKKAEEKKEEAKKEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CF64</accession>
    <name>A0A3B4CF64_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CF64"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009134"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015452.2">
        <property type="protein sequence ID" value="ENSPNAP00000009134.2"/>
        <property type="gene ID" value="ENSPNAG00000003214.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000285894"/>
    <dbReference type="OMA" id="NESEHFQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009134.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="278" mass="30977" checksum="9D3C8E57D734C5FB" modified="2024-11-27" version="2">AHSSRLELSELNLTHRILLNPTDTQQKSSLDPTDTQQKSSLDPTDTQQKSSVNPTDTQQNSSLDPTDTQQKSSVNPTDTQQKSSLDPTDTQQNLNPTDTQQKFSLDPTDTQQKSSLDPTDTQQKFSLDPTDTQQKFSLDPTDTQQKFSLDPTDTQQKSSLNPTDTQQKFSLNPTDTQQKSSLDPTDTQQKFSLDPTDTQQKCSLNPTDTQQKTRSVEPSTYPSEHDMNIQCGQIFQTLVWTPDEVAEGGSDKKKSPRGMRNPSSSGQQCAMELQQAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJ54</accession>
    <name>A0A3B4CJ54_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJ54"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036133.2">
        <property type="protein sequence ID" value="ENSPNAP00000011428.2"/>
        <property type="gene ID" value="ENSPNAG00000017083.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267613"/>
    <dbReference type="OMA" id="HICSDIC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903037">
        <property type="term" value="P:regulation of leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050863">
        <property type="term" value="P:regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF130">
        <property type="entry name" value="BUTYROPHILIN-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="39"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="173"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011428.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="271" mass="30727" checksum="CD29B9D98A0EEC36" modified="2024-11-27" version="2">MLGEKKEEMSVIHLHVSEFKLVSRSEDVGKPSHVEPLKPTSKQQSSPDFGPHVFCGHDVTLPCYLSPETSAVAMEIRWFKGTDCIYLYLNGQVTEGRGYEGRVSMFTDELQRGNVSLSLRDVQRSDYGEYRCEVTHGGHRVKNDGVRIKEFKLFSLPENYHSLIPQPFTVRACAGNDVLLPGFLSPKTSAVAMEIRWFKGTDCLYLYQNGQVTEGRGYEGRVSMLTDELENGNVSLKLKDYQSDGGEYRCEVTYGKHKMENKVHLHIEGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJS9</accession>
    <name>A0A3B4CJS9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactomedin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017557027.1">
        <property type="nucleotide sequence ID" value="XM_017701538.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJS9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017921.2">
        <property type="protein sequence ID" value="ENSPNAP00000011086.1"/>
        <property type="gene ID" value="ENSPNAG00000016730.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429647"/>
    <dbReference type="CTD" id="334035"/>
    <dbReference type="GeneTree" id="ENSGT00940000159148"/>
    <dbReference type="OrthoDB" id="8626508at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022082">
        <property type="entry name" value="Noelin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF27">
        <property type="entry name" value="NOELIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12308">
        <property type="entry name" value="Noelin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017380262" description="Olfactomedin-like domain-containing protein" evidence="10">
        <location>
            <begin position="17"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="11">
        <location>
            <begin position="224"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="225"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="483" mass="55151" checksum="9976B29781E5263B" modified="2018-12-05" version="1">MSVPLLKIGAVLSTMAMVTNWMSQTLPSLVGLNGTTISRGGTSERIVSALYPSPEEGWQIYSSAQDADGKCICTVVAPAQNMCNRDPRSRQLRQLMEKVQNITQSMEVLDLRTYRDLQYVRDTENLMKTVDGRLKTASENPRSLNPKSFQDLKDKVTQLLPLLPVLEQYKTDARMILRLREEVRNLSLVLMAIQEEMGAYDYEELRQRVLLLETRLHSCMQKLGCGKLTGVSNPITIRASGSRFGSWMTDTMIPSSDNRVWSMDGYYKGRRVLEYRTLGDFMKGQNFVQHLLPHPWAGTGHVVYNGSLYYNKYQSNILIKYHFRSRSVLVQRSLSNAGYNNTFPYSWGGWSDIDLMADENGLWVVYTTIPNAGNIVISRLEPQSLEVLQTWDTGFPKRSAGESFMICGTLYVTNSHLAGAKIYFAYYTNTSTYEYTDIPFHNQYSHISMMDYNPRERVLYTWNNGHQVLYNVTLFQVIKTAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CM00</accession>
    <name>A0A3B4CM00_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosinase</fullName>
            <ecNumber evidence="4">1.14.18.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Monophenol monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TYR</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Melanosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tyrosinase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.18.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CM00"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011856"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018935.2">
        <property type="protein sequence ID" value="ENSPNAP00000011856.1"/>
        <property type="gene ID" value="ENSPNAG00000017526.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155336"/>
    <dbReference type="OMA" id="HNGDYHM"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033162">
        <property type="term" value="C:melanosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004503">
        <property type="term" value="F:tyrosinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042438">
        <property type="term" value="P:melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043473">
        <property type="term" value="P:pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1280.10:FF:000003">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF124">
        <property type="entry name" value="TYROSINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00497">
        <property type="entry name" value="TYROSINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0186">Copper</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0470">Melanin biosynthesis</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017373279" description="Tyrosinase" evidence="19">
        <location>
            <begin position="20"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="475"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="20">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="21">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="537" mass="60959" checksum="D5FEF8FD4F5C5C2D" modified="2018-12-05" version="1">MWLLALITLVVQLLKPAYQQFPRQCVTREVLASKECCPIWEGDGSACGARSGRGVCQDVVVSEEPNGPQFPFEGIDDRERWPLVFYNRTCRCLQNYMGYNCGECSFGYFGPNCGERRETIRRNVFQLSTAEKQRFIAYLNLAKNTVSQDYVIATGTYSEMNNGTNPLFRNVSVYDLFVWMHYYVSRHALLGGTSNIWMGVDFGHWAPAFLPWHRVFLLRWEHEIRKLTGDFSFTIPYWDWRDAQGCEVCTDNLVGSQSRLNPNLISPSSVFSSWKVICSRAPEYSLRGVLCDGREEGPLLRNPGNHDRNLADGLPTSAEVDFTLSLSQYDTGPMDRTANMSFRNTLEGFGNPRTGLGNSPKLGMHAALHVFMNGSMSSVQGSANDPIFILHHAFVDSIYEQWLRRHQPESSHYPQANAPIGHNSEYYMAPFLPLYRNIDYFLSSKEMGYEYSYLQNPGQRFVDDVVSYLEEARVLWPWLLGAAVLGALCSTAMAVCLAKVSKSMWRCRSRRQGSEKQPLIISTDTEGSSSSYRTLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRZ2</accession>
    <name>A0A3B4CRZ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Receptor tyrosine kinase-like orphan receptor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ROR2</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552844.1">
        <property type="nucleotide sequence ID" value="XM_017697355.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CRZ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014145"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037214.2">
        <property type="protein sequence ID" value="ENSPNAP00000014145.1"/>
        <property type="gene ID" value="ENSPNAG00000020081.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427301"/>
    <dbReference type="CTD" id="4920"/>
    <dbReference type="GeneTree" id="ENSGT00940000153947"/>
    <dbReference type="OrthoDB" id="2431000at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000002">
        <property type="entry name" value="Inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000116">
        <property type="entry name" value="inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000003">
        <property type="entry name" value="Inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000139">
        <property type="entry name" value="inactive tyrosine-protein kinase transmembrane receptor ROR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016247">
        <property type="entry name" value="Tyr_kinase_rcpt_ROR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF132">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE TRANSMEMBRANE RECEPTOR ROR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000624">
        <property type="entry name" value="TyrPK_TMrec_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4 20" id="KW-0420">Kringle</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 23" id="KW-0732">Signal</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017420972" description="Receptor tyrosine kinase-like orphan receptor 2" evidence="23">
        <location>
            <begin position="32"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="27">
        <location>
            <begin position="54"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="25">
        <location>
            <begin position="166"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="26">
        <location>
            <begin position="312"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="471"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="754"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="853"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="763"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="857"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="868"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="900"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="613"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="477"/>
            <end position="485"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000624-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000624-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="30">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="935" mass="104291" checksum="1C5EDB0B5B3664AA" modified="2018-12-05" version="1">MGTERITRGILIHSFHIFILALCVLDASGSAAEVNLSESGEIEPLAEAQSGGLPTAEGYFLEFVEQPSNMTIMQGQAATLHCKVAGHPRPTIRWLKNDVPVVQEQGRISIRKTEFGSKLRIQDLDTTDTGYYQCVASNGLKVISATGVLYVRLGQSPTHGPDEATHEKGFCQPYRGIACARFIGNRSIYVESLQMQGESENRITAAFTMIGTSTQLSDQCSEFAIPSFCHYVFPLCEEGTRGPRQRQLCRDECEALENDLCRTEYTIARSNPLILMQLELPNCNLLPHPGSPDAASCMRIGVPVDHLETYHSCYNGSGANYRGTVSVTKSGYQCQPWSAQYPHNHHITPVEYPELRGGHNFCRNPGGQMESPWCFTLDPRVRVDLCDIRSCKPPENVKKEILYILIPSITIPLVIACLFFLVCMCRNKQKESVDTPPRRQLSASPSQDMEMPLLNQHKHQGKLREINLSAVRFMEELGEDRFGKVYKGHLYGTAPGEQTQVVAIKTVKDKDEGSLREEFRHEAMLRSRLQHPNIVCLLGVVTKEQPMSMIFSYSGHGDLHEFLVMRSPHSDVGSSDDDKTVKSALEQADFLHIVTQVAAGMEYLSNHHVVHKDLAARNILVCDKLNVKILDLGLFREVYSADYYKLMGSNPFPIRWMSPEAIMYGKFSTDSDIWSYGVVLWEIFSYGLQPYCGYSNQDVIEMVRNRQVLSCPDDCPSWIYTLMLECWNEFPARRPRFKDIHARLRTWESLSNYNSSAQTSGASNTTQTSSLSTSPVSNISAARYVTPKKASPFPQPQFMPMKGQMRPMVPPQLYIPVNGYQPVPAYPYLQNFYPMQIPMQMPSQQMHPQMVAKAGSHHSGSGSTSTGYVTTAPSNASGTEKAGLLSEDAKAAEENMADGASQNGLHNEDNSVPETELLGDNGIPQIDDCEMHSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSK9</accession>
    <name>A0A3B4CSK9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CSK9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022239.2">
        <property type="protein sequence ID" value="ENSPNAP00000014365.2"/>
        <property type="gene ID" value="ENSPNAG00000020272.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like"/>
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        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="10"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014365.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="130" mass="15068" checksum="4CF3462C460E5FD0" modified="2024-11-27" version="2">SCLFAWPSSYSDKTYYLVKERRNWTDAKSHCEQNYTGLAVIQNEGDWQRASAAVESTTNEVWIGLHRDPEWKWSNGEDLMYFNLALKQVCKKHFLTAVCQAAELLLSWILELQHYFTVSLKSFFLCSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CUA4</accession>
    <name>A0A3B4CUA4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ZFYVE19</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017558732.1">
        <property type="nucleotide sequence ID" value="XM_017703243.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CUA4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014975"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037533.2">
        <property type="protein sequence ID" value="ENSPNAP00000014975.1"/>
        <property type="gene ID" value="ENSPNAG00000020945.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430638"/>
    <dbReference type="CTD" id="84936"/>
    <dbReference type="GeneTree" id="ENSGT00390000016108"/>
    <dbReference type="OMA" id="CYRECHD"/>
    <dbReference type="OrthoDB" id="5407799at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009838">
        <property type="term" value="P:abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044878">
        <property type="term" value="P:mitotic cytokinesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19817">
        <property type="entry name" value="Bbox1_ANCHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15749">
        <property type="entry name" value="FYVE_ZFY19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000701">
        <property type="entry name" value="Zinc finger FYVE-type containing 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044553">
        <property type="entry name" value="Bbox1_ANCHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46603">
        <property type="entry name" value="ABSCISSION/NOCUT CHECKPOINT REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46603:SF1">
        <property type="entry name" value="ABSCISSION_NOCUT CHECKPOINT REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="238"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="436" mass="48746" checksum="080B4FAC501E2865" modified="2018-12-05" version="1">MNNRCYGCASKFTLFKKEVGCKGCGHSFCSGCLTYNALVPRFGNTQQKVCRQCHGNLTSGGKQNDAARWSPPENYKKRVAALEAKQTRNEPLRQGSGKNSTPALTSTKGLSKEDRVIAERLQKLKEETKPKSIPSEKDIESRLAALKAPIQPVPSAREMEDRLAALQGRPPPSQAPPPVHQPPDTRTQTEQANDLLTQMTEEVAIDNQPSPEANNSEGPVNSLNDLNKYETGTVDENLDDEQLSLKRMEEEKSRLLAEAMEELKHEDHSQEQLLQMAKRLAILQGRDPDKVTMTDFQQPDSDEETEEEAISRVLKRLSEEAALDEASGYNIPPERSGPENKEPSKPKSKKKQGPAQSSKSKPPATASVRQGSDSEDDDEEELPWCCICNQDATIRCHTCDRDLYCNRCFREGHDKYDRKEHHTSSYTAPKKAKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CW61</accession>
    <name>A0A3B4CW61_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DnaJ homolog subfamily C member 5B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">DNAJC5B</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with the chaperone complex consisting of HSC70 and SGTA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017572255.1">
        <property type="nucleotide sequence ID" value="XM_017716766.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CW61"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015578"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023834.2">
        <property type="protein sequence ID" value="ENSPNAP00000015578.1"/>
        <property type="gene ID" value="ENSPNAG00000021634.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438744"/>
    <dbReference type="CTD" id="85479"/>
    <dbReference type="GeneTree" id="ENSGT00940000159294"/>
    <dbReference type="OMA" id="EINKAHT"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000017">
        <property type="entry name" value="dnaJ homolog subfamily C member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051434">
        <property type="entry name" value="DnaJ_C_subfamily_member5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027:SF6">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="15"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="193" mass="21848" checksum="4519B11A48E9F159" modified="2018-12-05" version="1">MTEQRQRALSTSGEALYQVLGLDKNCTHDDIKKSYRKLALKYHPDKNPENPEAAEKFKELNNAHAVLSDVTKRNIYDKYGSLGLYVSQQFGEENVNTYFMLSSWWAKGLFAICGLLTGCYFCCCLCCCFNCCCGKCKPRTPGEEDPECYVSPEDLEEQIRTDVERDGDTPVVHQPTNASEKTQLIGDGHRTYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZD9</accession>
    <name>A0A3B4CZD9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AJUBA</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574227.1">
        <property type="nucleotide sequence ID" value="XM_017718738.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZD9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015914"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024254.2">
        <property type="protein sequence ID" value="ENSPNAP00000015914.1"/>
        <property type="gene ID" value="ENSPNAG00000022017.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440034"/>
    <dbReference type="CTD" id="84962"/>
    <dbReference type="GeneTree" id="ENSGT00940000160978"/>
    <dbReference type="OMA" id="VPPHNTH"/>
    <dbReference type="OrthoDB" id="25414at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035331">
        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09352">
        <property type="entry name" value="LIM1_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09355">
        <property type="entry name" value="LIM2_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09438">
        <property type="entry name" value="LIM3_Ajuba_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000037">
        <property type="entry name" value="LIM domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047172">
        <property type="entry name" value="Ajuba-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047245">
        <property type="entry name" value="Ajuba-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047247">
        <property type="entry name" value="Ajuba-like_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047248">
        <property type="entry name" value="Ajuba-like_LIM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24219:SF8">
        <property type="entry name" value="AJUBA LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24219">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN JUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="491"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="556"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="617"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="229"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="445"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="695" mass="75011" checksum="97653EB96AF5F0FC" modified="2018-12-05" version="1">MERIGTKLKQKFKLPDSGSVKFSKKKNELANTNNNSNSNSDTSAAPVVAHLNPTNSPALPSPAQFSSAEACGSTLSGRPSKVVNSQRVSPCVHAAATSASVPEESGPVKRRSPQQRASCYLPESVEGRGTCDQVRRGELSQGEHGDSQGAYSPNSRAALNQRRYSLELQQLVRRQQLLSQPPLSSMPPPYPTHGPVARSGSAEPSFLPEPERHKRLSLQEALFYKRWSSGGEPWDSGRPASLSHPPQRAHDSPGGGYLFPPGPTLSPCSSFSLQESVLASPRSSFASSTASGGGGGGSGSPMGSPCSSNRTSGISLGYDTRHTTPGPAAQQLQFSSTQLGGTTSGQVYPASGLGKAGAPPMEVWRDYLERGFQDGRHSYPPAVSVPTATGRRAEPEWWSGSEQRGERVSERIRHSDLPGTQYQQELTRLLLRDMALEGEELLGGLTLKEQPMSTSTPSSASAAPAGKSQQEEHPTVREPPQSALDRQEFFGTCVKCGKGVYGADNACQALDSLYHTRCFTCVSCGRTLRNKDFYNVSGSVYCKEDYMFSGFQAAAEKCNVCGHLILEQILQALGNSYHPGCFRCTVCSKTLDGVPFTVDYLNNVYCVSDYNRTFAPKCAACLQPILPAEGSEEILRVVSMNKDYHFECYHCEECGKQLSDKPGSQCFPLDSHLLCHSCHMSRVCATHSLPPQNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2A7</accession>
    <name>A0A3B4D2A7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">DYNLT3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2A7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038359.2">
        <property type="protein sequence ID" value="ENSPNAP00000017004.2"/>
        <property type="gene ID" value="ENSPNAG00000023210.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263473"/>
    <dbReference type="OMA" id="IDTNKTW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="25"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017004.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="142" mass="16046" checksum="4E4EB7C63973CF2F" modified="2024-11-27" version="2">IHRETQSNTETQTDRNTTVCIICHTGKYCSHPSLEIPLILINQKKTWREALSYCRVNHVDLVSVHTEEIQHWVETAVYYASTANVWMGLRHTCTLSFWFWVNGVSICYQNWAPGNGTGVEDCSGGERTGVVQSGSKFSVMCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2H8</accession>
    <name>A0A3B4D2H8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580499.1">
        <property type="nucleotide sequence ID" value="XM_017725010.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2H8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017281"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026103.2">
        <property type="protein sequence ID" value="ENSPNAP00000017281.1"/>
        <property type="gene ID" value="ENSPNAG00000023507.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444046"/>
    <dbReference type="CTD" id="221"/>
    <dbReference type="GeneTree" id="ENSGT00940000155904"/>
    <dbReference type="OMA" id="PLVAYWF"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004028">
        <property type="term" value="F:3-chloroallyl aldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07132">
        <property type="entry name" value="ALDH_F3AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF2">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE FAMILY 3 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="5"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="473" mass="52579" checksum="34671D7059AD295E" modified="2018-12-05" version="1">MESQKQVIERLRTAFRSGVTTPLQFRLKQLEALLSLLEDNEPQILEAMHKDLAKPKFEAVLSEVDLVVNDLCHTISNLHSWMQPSYVGKNLATKFDDCFVRREPLGLVLIIGAWNYPLQLIVAPLIGAIAAGNCAILKPSEISKATEQLLSALIPKYLSQECYAVICGGAEETKLLLENRFDHIFYTGSQAVARSILQAAAVYLTPVTLELGGKCPCLIYGRLDIKAGAKRLVWAKFFNVGQSCVAPDYVLCTTEMRDALVPAIREALEEFYGPQAQQSPDYGRIITDRHYDRLMELLGKSEGKVVIGGDGAKEDRYMAPTVIVDLKESDALMQEEIFGPILPIITIESLDQGISFINQREKPLALYVFSDESQIVTTVLERTSSGGFCSNDGIVHMTLPGLPFGGVGASGMGSYHGRWTFETFSHRRGCMLRGWGLERISMLRYPPYSERRLGWLRWATTAKKKGWAGCSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2V6</accession>
    <name>A0A3B4D2V6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2V6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026861.2">
        <property type="protein sequence ID" value="ENSPNAP00000017880.2"/>
        <property type="gene ID" value="ENSPNAG00000006517.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193390"/>
    <dbReference type="OMA" id="FREAICP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="87"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="160"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017880.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="30142" checksum="53EA000FF20443FC" modified="2024-11-27" version="2">MASSLSSLEEDLKCVLCQNVYKTPVMLLCTHSFCKSCLETSWAERETKECPVCKKRSSIENPPVNRALKSACDSFIQEKSRRVSEEERGLLCPQHLLNMQLFCLHDEQLVCAECVTQQHNNHNFCSIGKAANERRVRGRRFLHTQMQMKKEFEKLHKFLHEEEEARISALKKEMERKSQKMKEQMEQMDETLASLSKRIKLIEEYMNVRDSLFLKVSAEYTVPDPELDSGALIDVAKHLGNLRYRVWEKMKDICPYCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8W3</accession>
    <name>A0A3B4D8W3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Visual system homeobox 1 homolog, chx10-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560415.1">
        <property type="nucleotide sequence ID" value="XM_017704926.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8W3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029989.2">
        <property type="protein sequence ID" value="ENSPNAP00000019873.1"/>
        <property type="gene ID" value="ENSPNAG00000005509.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431637"/>
    <dbReference type="CTD" id="30813"/>
    <dbReference type="GeneTree" id="ENSGT00940000160793"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048318">
        <property type="term" value="P:axial mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030900">
        <property type="term" value="P:forebrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048329">
        <property type="term" value="P:negative regulation of axial mesodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048348">
        <property type="term" value="P:paraxial mesodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021501">
        <property type="term" value="P:prechordal plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000065">
        <property type="entry name" value="Visual system homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023339">
        <property type="entry name" value="CVC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052294">
        <property type="entry name" value="VSX_homeobox_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46892:SF2">
        <property type="entry name" value="VISUAL SYSTEM HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46892">
        <property type="entry name" value="VISUAL SYSTEM HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51496">
        <property type="entry name" value="CVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0844">Vision</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="135"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="CVC" evidence="15">
        <location>
            <begin position="197"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="137"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="107"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="251"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="326" mass="35295" checksum="48C6C9AA8A8A24AA" modified="2018-12-05" version="1">MEKTRAHGPGGLRAKGFAITDLLGLDADGASGGGGGGIGGACPSAVAGACPGGGVGAVGAFPFPLGLGFLCSLAAQQQQQQHQQQSARGFIPTHVPLLHARAEPPYAEQREEFSDDDCLSGDRNDGKSSGSSQKRKKRRHRTVFTSHQLEELEKAFNEAHYPDVYAREMLAMKTELPEDRIQVWFQNRRAKWRKREKCWGRSSVMAEYGLYGAMVRHSIPLPESIINSAKNGMMSSCAPWLLGMHKKSLEITKKSPGTPESTHSDSYSEDVKGKNSDLLWSGGPTAVDDPEDMAIDLSSTAKQETKSTLKTSPIHTEPNSDSENEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9A2</accession>
    <name>A0A3B4D9A2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9A2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020080"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030450.2">
        <property type="protein sequence ID" value="ENSPNAP00000020080.2"/>
        <property type="gene ID" value="ENSPNAG00000026985.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162740"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF257">
        <property type="entry name" value="GLYOXYLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="3">
        <location>
            <begin position="117"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020080.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="343" mass="38229" checksum="89E96731F7E5CC52" modified="2024-11-27" version="2">RVFIALKGIKNLNTVYCFYCLVFGKTKEKPCILDTIGQAGINKCFSSLLTRSNHLCVGSPLKVDRSLLQYLPKLKVVVNGGVGVDHLDIPLINSFGVKVYNTPHVVDNALADIGVNLMLACARKILQGTIYNLFIYHESETLLGSLLGNNVRGTTLGRIGYKVAKRAQSFDMKILYHSRSQRTEEEEKAVGAMYCEKMEELLKKSDFVTVVVNLSPQTQKLIGAKELAVMKPSSTLINIRRGKHHRDVLVDALQRKVIKAAALDVIDPEPLPTHPLLSFPNMIVGTHAVETTWVKVETLAALNDEQPPNEVKIQARPDTHHALHPSRATSNQYWFKVENCRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9M8</accession>
    <name>A0A3B4D9M8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017543485.1">
        <property type="nucleotide sequence ID" value="XM_017687996.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9M8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020148"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030516.2">
        <property type="protein sequence ID" value="ENSPNAP00000020148.1"/>
        <property type="gene ID" value="ENSPNAG00000006208.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415042"/>
    <dbReference type="GeneTree" id="ENSGT00400000024763"/>
    <dbReference type="OMA" id="WVIHPLS"/>
    <dbReference type="OrthoDB" id="8997642at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19379">
        <property type="entry name" value="TGF_beta_GSDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017455549" description="TGF-beta family profile domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="5">
        <location>
            <begin position="91"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="210" mass="23091" checksum="1A9983A3A2106E2D" modified="2018-12-05" version="1">MFLLLFTSVVLVGFPFGKTFILQPEEPATSPAIRTNRCQAESLQSVRKVILDLLNLQAEPHVSIPGMAAIRDQWKVAFKATSQSEPTQLNNSENQTSSQYSSPPNSTVLQCCKLASQIFIRDLGWDAWIIYPESFTYIQCSVCDPQVDQSITHCSGDRPPAPQVPCCEPTKQHLTPFLHLDHTGSLVIASVPLARECGCRPGTGPQTPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCF0</accession>
    <name>A0A3B4DCF0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cdk5 and Abl enzyme substrate 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CABLES1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCF0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020619"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031419.2">
        <property type="protein sequence ID" value="ENSPNAP00000020619.2"/>
        <property type="gene ID" value="ENSPNAG00000027502.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000022086"/>
    <dbReference type="OMA" id="RINRREM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000020">
        <property type="entry name" value="CDK5 and ABL1 enzyme substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012388">
        <property type="entry name" value="CABLES1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896:SF1">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025798">
        <property type="entry name" value="Cables"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="6">
        <location>
            <begin position="217"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020619.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="323" mass="36853" checksum="CCA2342142705136" modified="2024-11-27" version="2">MHRCSTGSPQPKSCKKVHFIKNMRQHDTRNGRIVLISVRRALYGMFSVVPYQEFSQLGDMRHFHSGMGTRDSVIGLVGVELGAEGKTVSYTEMLYPTNALCGHISNTASSTCSPCSQSPVRGVHSQGSRDKGTEVRDRVDYDPNLLDDPQWPCGKHKCVLVFHSYMTTVIEYVKPSDLKRDMNETFSEKFPHVQLTLSKIRSLKKEIRKLAQEDCGYEEPTVAMAFVYFEKLALNGKIDKQNRKLCAGACILLAAKIGSDLRRPEVKSLIDRLEEKFRLNRKDLLAFEFPVLVALEFKLHLPHHEILPHYRRLLLTSYNQKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DIX4</accession>
    <name>A0A3B4DIX4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Coiled-coil domain containing 136b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037396712.1">
        <property type="nucleotide sequence ID" value="XM_037540815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DIX4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024337"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010392.2">
        <property type="protein sequence ID" value="ENSPNAP00000024337.1"/>
        <property type="gene ID" value="ENSPNAG00000008840.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428076"/>
    <dbReference type="CTD" id="100330186"/>
    <dbReference type="GeneTree" id="ENSGT00940000159122"/>
    <dbReference type="OMA" id="HEGSQRE"/>
    <dbReference type="OrthoDB" id="9948923at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002080">
        <property type="term" value="C:acrosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF26">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="377"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="54390" checksum="06BDE7BE0E7CD384" modified="2018-12-05" version="1">MDCGLRLPPVIEEVIDPADDLCELKVERPGNELTAKERESLEENDEKEEKEVEVGNGEKGVEEGNGEKGLKEEKEVEEEEKAGDGERVRGLEEEGIEVEELRAQVVQLLLELEEAREVSQRHEDSYNELQGLLEEERLASAHQAETFTRQIQRLQAQLRSVQEELDSLEEEKASELWEAQEELRAAQVEVQELQQAAEEAAAERENDIALLQEELCRLRAELQRLHSTTQEYELELTTLRAEISMKSQHREQQDQANTEGEVGQLKEECRTLMDECQTLRNDNRQLSEKLHLLEQQKASSTDSYLAVRGEQDNSESARKNKMGKMEGEEKAVKAEGYMSMLQPEQSSRVVDDSIQKNISTEDRTVNPMERSVGLSEVFSLRDRLKQAEERAFQVQRECEGLKGELLELQGLYERSQRERAGLEAELQRCRAELDRLAGRKPQNSSPPSESPVLSIPFIGMVVIMALIWCCWTELLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DL65</accession>
    <name>A0A3B4DL65_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537636.1">
        <property type="nucleotide sequence ID" value="XM_017682147.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DL65"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025132"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008372.2">
        <property type="protein sequence ID" value="ENSPNAP00000025132.2"/>
        <property type="gene ID" value="ENSPNAG00000010059.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410853"/>
    <dbReference type="CTD" id="560560"/>
    <dbReference type="GeneTree" id="ENSGT01140000282547"/>
    <dbReference type="OMA" id="AYGEAHE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="CDD" id="cd10024">
        <property type="entry name" value="14-3-3_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000001">
        <property type="entry name" value="14-3-3 gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="4"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025132.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="247" mass="28278" checksum="05CB855645D42323" modified="2024-11-27" version="2">MVDREQLVQKARLAEQAERYDDMAAAMKSVTELNEALSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLNLLDNFLIKNCSDTQHESKVFYLKMKGDYYRYLAEVATGEKRSTVVESSEKAYNEAHEISKEHMQPTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDEGGEGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DM14</accession>
    <name>A0A3B4DM14_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Accepts the ubiquitin-like proteins sumo1, sumo2 and sumo3 from the uble1a-uble1b E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as ranbp2 or cbx4. Essential for nuclear architecture and chromosome segregation. Mediates nuclear localization of vsx1. Required for progression through mitosis during organogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a tight complex with rangap1 and ranbp2. Interacts with vsx1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563041.1">
        <property type="nucleotide sequence ID" value="XM_017707552.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DM14"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025437"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007604.2">
        <property type="protein sequence ID" value="ENSPNAP00000025437.1"/>
        <property type="gene ID" value="ENSPNAG00000010538.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433161"/>
    <dbReference type="CTD" id="114445"/>
    <dbReference type="GeneTree" id="ENSGT00550000075088"/>
    <dbReference type="OMA" id="TWECGIP"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036306">
        <property type="term" value="P:embryonic heart tube elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000013">
        <property type="entry name" value="SUMO-conjugating enzyme UBC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="157" mass="17926" checksum="FAEE89C8D0683DFA" modified="2018-12-05" version="1">MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPC0</accession>
    <name>A0A3B4DPC0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573061.1">
        <property type="nucleotide sequence ID" value="XM_017717572.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPC0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054081.1">
        <property type="protein sequence ID" value="ENSPNAP00000060957.1"/>
        <property type="gene ID" value="ENSPNAG00000031990.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108439276"/>
    <dbReference type="CTD" id="798390"/>
    <dbReference type="GeneTree" id="ENSGT01050000244887"/>
    <dbReference type="OMA" id="KWDRPRS"/>
    <dbReference type="OrthoDB" id="8934207at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038036">
        <property type="term" value="F:sphingosine-1-phosphate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004061">
        <property type="entry name" value="S1P_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01523">
        <property type="entry name" value="S1PRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="423" mass="45624" checksum="FAF138B7B22BBD79" modified="2018-12-05" version="1">MHLAGSQSSAHRRPLLFEPRSAIRMDIYLEEGMNSVIVTHYNHSGKWGRPRSGSTCKMAVQLLICVLIVLENATVLLALRRNKRFHSRMYLLIANLALSDLLAGVAYIVNLITSGERTFYLTPTQWLAREGSMFLALSASTFSLLAIGVERHMTMVRLRPCEAAAGGRGRLLALLAACWAVSLLLSALPSLGWNCLGQLHTCSTVLPLYAKSYVAFCISVFSALLAAVVALYVRIYRLVTSSGRRVGSRPSERSLALLRTVVIVLGVFVACWSPLFLLLLLDVGCGPERCPVLYQVEWFIVLAVLNSALNPLIYTLSSREMRAAFFRLLCCCCCQTSLDAVPTTAGPPLPGTCMPTAENSKSSAGGVGSGAVRSTLMRSKVPTPANSQHRDSSTPAVTHPSGPADLLSAVLVKAGALPSLSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DRB0</accession>
    <name>A0A3B4DRB0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LCLAT1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576478.1">
        <property type="nucleotide sequence ID" value="XM_017720989.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DRB0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025995"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006236.2">
        <property type="protein sequence ID" value="ENSPNAP00000025995.1"/>
        <property type="gene ID" value="ENSPNAG00000001633.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441453"/>
    <dbReference type="CTD" id="253558"/>
    <dbReference type="GeneTree" id="ENSGT00950000182836"/>
    <dbReference type="OrthoDB" id="186786at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="98"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="401" mass="46683" checksum="F632B5151BB4813F" modified="2018-12-05" version="1">MRAEVRRSGRVNVTFPGMAMVSVRGLYFVVTLFLGSFFGSVFMLGPVLPLMLLSPAWYRWLTDRIVATWLTLPVALLEVVFGVKVVITGDGFVPGERSVIIMNHRTRLDWMFLWCCLLRYSYLRLEKVCLKAALKAVPGFGWAMQVAAFIFIQRRWEEDRSHMENMLEYFCDIREPLQLLLFPEGTDLTDNTRARSDEFAEKNGLPKYEYVLHPRTTGFTFIVDTLRKGNNLDAVHDVTVAYPQNIPQTERHLMLGLFPREIHFHVRRYPLSTLPEGSDLLQAWCQDRWTEKEQRLREFYGASPRLFDDPEARVPPCKSELRVTLVKAVSLCYWTLFITLSFVGLWLWAPLRMYFLLVVAFFLLQQKVMGGVEMMELACHRHWNGKPPMSREKDGPRVKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTE8</accession>
    <name>A0A3B4DTE8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Olfactomedin 4-like 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTE8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026254"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005569.2">
        <property type="protein sequence ID" value="ENSPNAP00000026254.1"/>
        <property type="gene ID" value="ENSPNAG00000011539.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165829"/>
    <dbReference type="OMA" id="CEVNSTM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF31">
        <property type="entry name" value="OLFACTOMEDIN-4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017477105" evidence="5">
        <location>
            <begin position="30"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="6">
        <location>
            <begin position="196"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="116"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="464" mass="53214" checksum="CBD70D5E33FFB0C0" modified="2018-12-05" version="1">MVLLKLVVTHRLTMILLVLLLTATQAGHAQRVLGEEKDGSCVCEVNSAIWAFPTAKYETVSELLEGCNATLQQLQTQVMMTNKELPKMQATVENIMARKQRFKYLDTQGLYNPLHLRQVSDELQDLHQIISQADKDRPTAETQKLRKELQKIQKDVEEMYNNNTFNLEAVKEKLRYLNNRVQTCRTIPADFTSKGSCSHRIMSNISSPLITKLSPFGKSYISGAWGRDTSAGSEEKYWVQPLVNSQRSGYQIRYYPSFEDFMAGRNHRDEYVASSYTHTDAIQGPGTIMYDGVVYYQCYTTAELCSYNLKAKKNNRLKLPEAGANNKFPYCYYNCYDWTDIDLAADSEGLWVMYATDGNYGNMVLSRVNREGFNVTQTWKTRLFKRSVTNAFMVCGVLYATRYVDQYKEEVFYAFDTATGLEDNTLSLPLEKVSAGIANLHYNPTDMKLYMYNDGYLLSFQVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTK1</accession>
    <name>A0A3B4DTK1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sorting nexin 19a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557264.1">
        <property type="nucleotide sequence ID" value="XM_017701775.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037389461.1">
        <property type="nucleotide sequence ID" value="XM_037533564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTK1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026780"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004263.2">
        <property type="protein sequence ID" value="ENSPNAP00000026780.1"/>
        <property type="gene ID" value="ENSPNAG00000002099.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429767"/>
    <dbReference type="CTD" id="100170806"/>
    <dbReference type="GeneTree" id="ENSGT00950000182856"/>
    <dbReference type="OMA" id="QESWTTC"/>
    <dbReference type="OrthoDB" id="5582218at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF33">
        <property type="entry name" value="SNX19A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="5">
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            <begin position="92"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="500"/>
            <end position="630"/>
        </location>
    </feature>
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            <begin position="287"/>
            <end position="332"/>
        </location>
    </feature>
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            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
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            <begin position="452"/>
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    </feature>
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            <end position="684"/>
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            <begin position="316"/>
            <end position="332"/>
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    </feature>
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        </location>
    </feature>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="938" mass="105215" checksum="1430D459E9F43267" modified="2018-12-05" version="1">MPGFEGTGPSSRTLPMLMGQRSIVGFVVLITWLVLFYLLVNVWLLCIFTSLLVVLGGWLGSRAALDANSLLHLEHFLPLGQAPLPHVSLESERRLDWEIHCAVEKAVRDFVSSWYRTLVSEEEGEFQQEIRDTMLTAAAELKRRARQVDRRVLAQRMLELCGCHLQSFSQAKGLQKVVLATSVQPQQHGPRKQVGQQGPRSTLWMLYSEVSAPHPALASSAAELCYSRAVMDLLLHILVPYPHLETRTGRYLVGELITCNVLLPLVAQISNPDWFNQTIVDVFTKSSGQDAEGTMEPPSPWDRDFHTPLPWDHSETSSMQTARSNSTLPLQESSTSSLQMLWSSEDYTKEGVTSESPYLERAAPSPPELLSSPYKSSQLYYNTDYGIESPLSDSNKISNESLYLNESEVDKDETFCDCINPAEFCGLVCLDDDTLALLGKRIRPKLLISEPLSRPESPMEDPFTGSLSQDTNSSPGGTRLSSFGLENLGSLEGQMVIKNLKITGTITAKEQKGNSSHPYTLYTVKYETVVGTDCLSTDQAIGYHTVNRRYSEFLNLQTRLEERSDLKKVIKNVKGPRKLFPDLSFGNPDTEKVEARKGQLESFLKKLCSIPEAAKSEEVQEFLALNTDASAAFEKKPCSSRIDKMVENIVDTLKTAFPRSEPQSPTDDGEPDVDRRSPDSRKKSRLRFSSIIASTTLSVPDFQPRITYCFSEGSTVFKGPLGIEGFVLEQEKRLDGEPRKNNRRGAEQEDKDGDRAAEKKTRGTDTALADVALNILCLLMKDQWSWLCTENIQKTIRLLFGTIIERWLEVGIAHLTSAPCWVIYLRVLQEAVWPGGDLPVGPQPERSPQQREETRLQCLDCLMKLFPELIPDILGCEKYKLSWEHMLESLQDPHINRHLIYCIFDLLLEFLVPESSEEGFQKSLLQSLSCDVEASVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUC6</accession>
    <name>A0A3B4DUC6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycoprotein integral membrane protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GINM1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017553233.1">
        <property type="nucleotide sequence ID" value="XM_017697744.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUC6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027068"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003521.2">
        <property type="protein sequence ID" value="ENSPNAP00000027068.1"/>
        <property type="gene ID" value="ENSPNAG00000012472.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427527"/>
    <dbReference type="CTD" id="116254"/>
    <dbReference type="GeneTree" id="ENSGT00390000008373"/>
    <dbReference type="OMA" id="ISAMPRE"/>
    <dbReference type="OrthoDB" id="10022429at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042319">
        <property type="entry name" value="GINM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28549">
        <property type="entry name" value="GLYCOPROTEIN INTEGRAL MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28549:SF1">
        <property type="entry name" value="GLYCOPROTEIN INTEGRAL MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017260988" description="Glycoprotein integral membrane protein 1" evidence="3">
        <location>
            <begin position="22"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="358"/>
        </location>
    </feature>
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        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="40284" checksum="A37368A44C0EDA1B" modified="2018-12-05" version="1">MASSWGMFSVVFSLTVVFISTFSETRQLSTENVINVTAVSENKESSYNVQINLSVAMVDNKTMVNGVSLKPSEVTRMTCQALLLDDNNVTLSDGAGELVSSVMRLMLDQSEMQSDAGEEVLLLTLGQEIIQLGQENVWQPDVWEMKMLLNQNSEEVTQVHDFFPMSQSKISAMPRENDIIMTDESIPETAEDQVLHTTSHYPLKQAETTQEEIAAPGKLPETPLRMDPSTLYESAQDETVNPDDLLPEVPLRQSPSSYNVVCRWVEELRDRLKRFCSESLPLFFLIMWVVVVGVAGSAVIIKILDIVFPSCEHKGFFHLNPETLLPVDDDDEEKQSLVESMEMERETEEKSGVREDSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUW7</accession>
    <name>A0A3B4DUW7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUW7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027245"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003065.2">
        <property type="protein sequence ID" value="ENSPNAP00000027245.2"/>
        <property type="gene ID" value="ENSPNAG00000012689.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287084"/>
    <dbReference type="OMA" id="TIPHANI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF96">
        <property type="entry name" value="GRANZYME A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="39"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027245.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="194" mass="21128" checksum="81F82FD4D9933879" modified="2024-11-27" version="2">MIQFSPAMMKQSRKMLKYTAVLLFTVSVSLVSVELWKRLIPGSDCSDDQRLHHVQVVYETKGSKSGACGGTLIHKQWVLTAGHCYKGTAGTLKVIVGVHPTNSEVNKTLTIPHANIKKYSGTLQDGDIMLLKLPESVENIKPAKLPDVPCTRPPARDMLQVAGHGSASKIYGKAEARLKCLNVSVVSCQNLKCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DYY1</accession>
    <name>A0A3B4DYY1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017557862.1">
        <property type="nucleotide sequence ID" value="XM_017702373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DYY1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029617"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016894.2">
        <property type="protein sequence ID" value="ENSPNAP00000029617.1"/>
        <property type="gene ID" value="ENSPNAG00000015850.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430122"/>
    <dbReference type="CTD" id="393998"/>
    <dbReference type="GeneTree" id="ENSGT00940000163111"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14209">
        <property type="entry name" value="STKc_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044109">
        <property type="entry name" value="STKc_PKA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF116">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0114">cAMP</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="83"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="338"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="390" mass="45483" checksum="946CDA64F0499E7A" modified="2018-12-05" version="1">MAQGRDQSQSMGTLQRLDTLASRFFYKYRKGNQEKSQESDGPHISEYTIIWDGTMKEFLAKAKEDFLRKWECPPQSTTCLDDFERLKTLGTGSFGRVMLVKHKGTEQYYAMKILDKQKVVKLKQIEHTLNEKRILQAVSFPFLVRLEYAFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEHHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQHGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYEKKVEAPIIPKCRGPGDTSNFDDYEEEEIRISDTEKCAKEFLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZ46</accession>
    <name>A0A3B4DZ46_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="6">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017579726.1">
        <property type="nucleotide sequence ID" value="XM_017724237.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017579727.1">
        <property type="nucleotide sequence ID" value="XM_017724238.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZ46"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035122.2">
        <property type="protein sequence ID" value="ENSPNAP00000028728.1"/>
        <property type="gene ID" value="ENSPNAG00000003164.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443501"/>
    <dbReference type="GeneTree" id="ENSGT00940000165122"/>
    <dbReference type="OMA" id="NQNYTDY"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zgc:174895"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenine_PRTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776:SF7">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="12">
        <location>
            <begin position="56"/>
            <end position="179"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="200" mass="21882" checksum="2C353E96E7845CFA" modified="2018-12-05" version="1">MDALAVPESGGEGWYLSLMAPNTKGPSFAWVDPSRLYCNSQALSDCVKDLLKPFQNDSIDLVVGIDAMGFILGSAVAITLGKGFLAIRKAGRLCVETQTQNYSDYSGEEKLIEVRVDVLRPGLRVLLVDQWIETGGTMIAAIKLVEKQGAAVVGVAAVAIENTEGGKWIKENYKYSHCIPKELRSQIDSKYLESFKTFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E185</accession>
    <name>A0A3B4E185_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peroxisomal membrane protein PEX14-like KPWE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E185"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030387"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018422.2">
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        <property type="gene ID" value="ENSPNAG00000017092.2"/>
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        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040554">
        <property type="entry name" value="KPWE_PEX14_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17733">
        <property type="entry name" value="KPWE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peroxisomal membrane protein PEX14-like KPWE" evidence="1">
        <location>
            <begin position="13"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030387.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="77" mass="8674" checksum="F69A486142E31823" modified="2024-11-27" version="2">MDNRIYKPPQGGPLSFAEVFRMIQAGEEIPGLQKLDIKPSNQQPTVSQMLRKLKPWEKTIATALENFHSVLHVSSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4B1</accession>
    <name>A0A3B4E4B1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RCC1L</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4B1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017930.2">
        <property type="protein sequence ID" value="ENSPNAP00000030161.2"/>
        <property type="gene ID" value="ENSPNAG00000005877.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053035">
        <property type="entry name" value="Mitochondrial_GEF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46337">
        <property type="entry name" value="RCC1-LIKE G EXCHANGING FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46337:SF1">
        <property type="entry name" value="RCC1-LIKE G EXCHANGING FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="52"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="53"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="116"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="238"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="291"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="344"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="402"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030161.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="453" mass="49336" checksum="212B7235889F3871" modified="2024-11-27" version="2">MCRDCFRVCGRYGFLPGCRGFATAGSAAPKRREPTDDRPVFQYVGEHRKPREKVFVWGFSCTGALGIPSFVVPDSGRKKPRRYQLTPYRLETEQKISSAACGYGFTLLASNTKDLTKVWGMGLNKDSQLGFQRTQHDRSKTLYDYVLEPSPVSLPLANPQETRVLQVSCGRAHSLVLTDSEGVFSMGNNAYGQCGRKIVEDEVYRFVSCLQLHTVFRISRCEAVACGQDHSLFLTETGSVYACGWGADGQTGLGHHNKTALPVLVRGDLAGVKIQQVTTYGDCSLAVSTDGQLFGWGNSEYLQLASVTEATQISSPQLLPLKGVGKVTQAACGGTQVAVLNEDGEVFVWGYGILGKGPNLSESEIPEKVPATLFGRSEFSPTVKVSQIRCGLTNFAAITDHGELFVWGKNVRGCLGIGKREDQYFPWRVTVPGHVVNVACGVDHMVALVKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4E5</accession>
    <name>A0A3B4E4E5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4E5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030191"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036003.2">
        <property type="protein sequence ID" value="ENSPNAP00000030191.1"/>
        <property type="gene ID" value="ENSPNAG00000016776.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009571"/>
    <dbReference type="OMA" id="HYFRISA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF48">
        <property type="entry name" value="KALLIKREIN-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="2">
        <location>
            <begin position="51"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="295" mass="32031" checksum="AD70836DFE536C73" modified="2018-12-05" version="1">MVGAEQVLKSDMFGLFSPVQELKTFNMEKTAFLILSLWAAGVTSSVVEKRLVSAITCPDTERLHHARVVYEFRDGKSMTCGGTLIDKRWVITASHCYGGEDGTLKVELGGHPKTAVKKQLRIASNNIILYAKSSSEEPIMLLKLPESVKNINPAVLPPGDCMAPTDGDELQVSGRGATTAEGGPGVKDLMCLEVKTLSKNHCPDLAGTSYEKFTHIYCGGGVGGAEIKACKGDSGSGFLKKEMISKSFFVFWSKEKKADVLYGVLISGHEACEDKFVFVDLCAKKIKKWIQKLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E5M5</accession>
    <name>A0A3B4E5M5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-alpha-acetyltransferase 35, NatC auxiliary subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein MAK10 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NAA35</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5M5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030539"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018685.2">
        <property type="protein sequence ID" value="ENSPNAP00000030539.2"/>
        <property type="gene ID" value="ENSPNAG00000017383.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002445"/>
    <dbReference type="OMA" id="QMEWIVQ"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="7">
        <location>
            <begin position="45"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="8">
        <location>
            <begin position="344"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="554"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="565"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030539.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="732" mass="84011" checksum="63D15B5ED6783420" modified="2024-11-27" version="2">MVMKSSVEEDEGGWGLGIPEKMRSNANWVDITQDFKGACKELKLGELLHDKLFGLFEAMSAIEMMDPKMDAGMIGNQVNRKVLNFEQAVKDQAIRVKDLSLPELIGIVDTCFCCLITWLEGHSLAQTVFTCLYVHNPELIQDPALKAFALGILKVCDIAREKVNKAAVFEEVKFIYCIEDFQAMTYGFKMANNVTDLRVTGMLKDVEDELQRKVKSTRSRQGEQRDPEVELDHQQCLALFSRIKFTRLLLTALISFTKKETSAVSEAQKLMSQAADLLPAIHSTIQYGIQSQNDTTKGDHPIMMGFEPLVNQRLLPPTFPRYAKIIKREEMVNYFSKLIERIKTVCEVINTTNLHSILDFFCEFSEQSPCVLSRSLLQTTFLIDNKKVFGTHLMQDMIKDALRYFVSPPVLSPKCSLYNNHQAKDYIDSFVTHCSRPFCSLIQIHGHNRARQRDKLGHILEEFATLQDEAEKVDAALHGLLMKLEPQRQHLACLGTWVLYHNLRIMIQYLLSGFELELYSMHEYYYIYWYLSEFLYAWLMSTLSRADSSQMAEERILEEQQQKGRSSKKSKKKKKARPLGKEITMSQAYQNMCAGMYKTMIALDMDGKVRKPLFELDSEQVRYEHRFAPFNSVVTPPPVHYIQFKEMSDLKKYSPPPRSADLYMAASKHFQQAKLILENVASPDAEVNRILKVAKPNIVVMKLLAGGHKKETKALPEFDFSAHKYFPIVKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E5U1</accession>
    <name>A0A3B4E5U1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547984.1">
        <property type="nucleotide sequence ID" value="XM_017692495.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5U1"/>
    <dbReference type="SMR" id="A0A3B4E5U1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031130"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019817.2">
        <property type="protein sequence ID" value="ENSPNAP00000031130.2"/>
        <property type="gene ID" value="ENSPNAG00000032860.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108424452"/>
    <dbReference type="GeneTree" id="ENSGT01110000267181"/>
    <dbReference type="OMA" id="KVKQMNY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031130.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="126" mass="13948" checksum="17FF749927C2070C" modified="2024-11-27" version="2">MPEPAKSAPAPKKGSKKAVTKTQKKGDKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E655</accession>
    <name>A0A3B4E655_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pentraxin (PTX) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537321.2">
        <property type="nucleotide sequence ID" value="XM_017681832.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E655"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030801"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036354.2">
        <property type="protein sequence ID" value="ENSPNAP00000030801.2"/>
        <property type="gene ID" value="ENSPNAG00000017745.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410647"/>
    <dbReference type="GeneTree" id="ENSGT01060000248591"/>
    <dbReference type="OMA" id="HHDNDEH"/>
    <dbReference type="OrthoDB" id="6515930at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00152">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000012">
        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF3">
        <property type="entry name" value="NEURONAL PENTRAXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043971851" description="Pentraxin (PTX) domain-containing protein" evidence="8">
        <location>
            <begin position="41"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="9">
        <location>
            <begin position="404"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="274"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="280"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030801.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="607" mass="66899" checksum="62776DAC31F2E4C8" modified="2024-11-27" version="2">MKLCSLHSMFIPRVRLQRSTDAWTIPLVLYVLLLLSGCAGSGMPGSEHTEYLSHPRFICTPIPADADPACFPSGAVPGAGGPSMNGHHSGHHSGPGMSGSAWWGMSEEAKATILHLRESLVQQKETILDQRETIRELTAKLTLCQGFGTTLGTHDSDHLHVPPHLAHHTYPTMPDSGHHGYAPDSGHHGTHATEPSHYGHHRGKAALGDKYTTSDLTPSPSSTPEQMGRMLMALKERLENLQTRNTSTTYSSSLKDLLQRKIAALEQQMLHHTNSLSSSNHHDNDEDDGDDHHSDHHDDSGHETHSDDHEHHDEDHEHHDGQHHDDDHHDDSQDHHDSSYDDVHLANGEHEDSHHDNKAAGHSYPRMSYHSQGHSGQSKLDAVLNHLTHRLSETGLRKKSKATGGFQIGFPMRTNYMYGRVKGTLLHEIFALTLCLWMKGGAGPGLGTPFSYSVPGQANELVLIEWGNNPMELLINDRAVTLPLSLSDGKWHHVCVTWSTRDGLWEAYQDGVKRGSGENLSAWHPIKPGGVFILGQEQDTLGGRFDATQAFVGEISDLQLWSHILTPHDIYSLASCGGHMTGDIITWAESVVELHGGVTKYPFDPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E7N8</accession>
    <name>A0A3B4E7N8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Large ribosomal subunit protein mL44</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ribonuclease III family. Mitochondrion-specific ribosomal protein mL44 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E7N8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020904.2">
        <property type="protein sequence ID" value="ENSPNAP00000031693.2"/>
        <property type="gene ID" value="ENSPNAG00000019170.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155511"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007289">
        <property type="term" value="P:spermatid nucleus differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000010">
        <property type="entry name" value="39S ribosomal protein L44, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052248">
        <property type="entry name" value="Arf-GAP_FG-repeat_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044444">
        <property type="entry name" value="Ribosomal_mL44_DSRM_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055189">
        <property type="entry name" value="RM44_endonuclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134:SF6">
        <property type="entry name" value="ARF-GAP DOMAIN AND FG REPEAT-CONTAINING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134">
        <property type="entry name" value="DRONGO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22892">
        <property type="entry name" value="DSRM_MRPL44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22935">
        <property type="entry name" value="RM44_endonuclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="13">
        <location>
            <begin position="237"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="582"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="744"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="586"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="744"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031693.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="809" mass="85796" checksum="DEFB26D5D1B4405B" modified="2024-11-27" version="2">MAACWRLLSRTVSVLGHGQVCRSVLLSQTREKKRWMKAYTLLMERKRRIEGEPAPKPRSQQPNWDYHAEVEAFSARLKESFSVELLKTAFVNPCYLRSEEERRRTLGLDAEGAALNLRDNTELREHGQQFTLNFLKDWCKSSFPNLPEAGVTAIVSHLTGSEVVCDVARNLAVEELAMAAEFPVPQDILRGTFFAVVGALEQSSGTEKARLFVRDFLVTQLIGKDLFDMWRVVNPMGLLVEELTRRGKALPEPRLIQSAGASTVLPLYFVGLYRRGLNPPHRVKSISMTTFTQQEIEFLQKHGNEVCKQIWLGLYDDRTSAIPDFREPQKVKEFLQEKYEKKRWYVPPEQAKVVASVHASISGSSASSTSSTPEVRPLKTLLGETAPTLHLSRNTPSQSPVVSRTQLTQQDRKFDLLSDLGGDIFAAPPSQSAGGANFANFAHFNSHPASQNANASADFANFDAFGSSAAAAAPSAAFPQAPQQATFQPAQTVLSALPSSRSVGEFPSVPPLLATHSGSSGQANANFANFDNFPKSCSADFASYGSSQTNCAGGGAKAEGFAVPADRYAALADLDNIFSSVKPDQGTSTGTGSSGASSAPPSAGVGPPPPQAAMLGGDRYAALAALDNVFSSSSSTTSSFNTVTNTQGTVFGASTGVSSAQPQQGSLPSIPQGFTGPSTNPFVATAVPQAAVPTNPFQTNGRAVAGAASFGPGSMSMPAGFGNAAAYNLPTSFSGSFQQAFPSQGFPQQQPYPQQPNGGGFAAFGGQGKTVVAPFGQNMASAGVTNNPFLAGAPAGQYPAGNSSTNPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8G9</accession>
    <accession>A0A3B4DY19</accession>
    <name>A0A3B4E8G9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8G9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016287.2">
        <property type="protein sequence ID" value="ENSPNAP00000029307.2"/>
        <property type="gene ID" value="ENSPNAG00000015319.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000037157.2">
        <property type="protein sequence ID" value="ENSPNAP00000031983.2"/>
        <property type="gene ID" value="ENSPNAG00000015319.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168178"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044589728" description="Ig-like domain-containing protein" evidence="12">
        <location>
            <begin position="27"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1369"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
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            <end position="124"/>
        </location>
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        </location>
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    <feature type="domain" description="Ig-like" evidence="13">
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            <begin position="234"/>
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        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
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            <begin position="356"/>
            <end position="437"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="458"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="560"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="662"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="764"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="866"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="968"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="1070"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="1172"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="1274"/>
            <end position="1353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031983.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1432" mass="160963" checksum="7DE40256139B4E3E" modified="2024-11-27" version="2">MNSGVLSVKMWLCVFLLYSVLQESSGCTVERGLGDTITRSAGDSVELPCSCTQQTKEDPQTVQWGFKRKFIQGDYNADHSVFQEDGSQNQRYRGRVQRLNQNKPGTVALIISHLTEEDEGAYLCGNNRKYNRAVILQVSTGCAVLSDSERTFSRSPGESVLLPCPCPPDQHRINPDRVTWTKDQTAVSNDTESYRGRVWMFDQNHSRNFSLLISDLTKEDSGRYTCRADEKDMPFVLLDVTGCTLSENKQTVPVSRSSGESVLLSCTCTDQQGRPVGVQWRTPNQEDLLQRYSARVQTSNQSSTGNFSVLISDLTEEDGGTYSCWINQNQYRNFSLTVKDCKLSETQEKVIHKYPGESVLLPCACSDQRTTPVSVKWERVDSGRTEVSNEMEPYKGRVQMFNKNLPANLSLLISNLTEQDQGTFRCSINNKHSINITLNIRDCTLSETQEKVIHKYPGESVLLPCTCSDQRTTPVSVKWEHVDSGGTEVSNNTANYTGRVQMFNKNVPANLSLLISNLTEQDQGTYRCSINNKQSINIRLNIKDCKLSETQEKDIHKYPGESVLLPCTCSDQRTIPVSVKWEHVDSGGTEVSNKTGNYTGRVQMFNKNLPANLSLLISNLTEQDQGTYRCLINNKQSINITLNIRDCKLSETQEKDIHKYPGEFVLLPCTCSDQHTKPVSVKWERVDSGRTEVSNEMELYKGRVQMFNRNLPANLSLLISNLTEQDQGTFRCSINNKQSINIRLSIRDCKLSETQEKDIHKYPGESVLLPCSCTDQRTTPVSVKWDHVDSGGTEVSNKMAKYTGRVQMFNKNLPANLSLLISNLTEQDQGTYRCSINNKQSINIRLNIRDCKLSETQEKDIHKYPGDSVLLPCACSDQHTKPISVKWERVDSGGTEVSNETPRYTGRVQMFNKNLPANLSLLISNLTEQDQGTYRCSINNKQSINITLNITDCKLSETQEKVIHKYPGESVLLSCSCSDQRTIPVSVKWEHVDSGGTEVSNKTANYTGRVQMFNKNLPANLSLLISNLTEQDQGTYRCSINNKQSINITLNIKDCKLSETQEKDIHKYPGESVLLPCSCSDQRTIPVSVKWEHVDSGGTEVSKKTANYTGRVQMFNKNLPANLSLLISNLTEQDQGTYRCLINNKQSINIRLNIRDCKLSETQEKVIHKYPGESVLLSCSCSDQRTIPVSVKWERVDSGGTEVSNKTANYTGRVQMFNNNLPANLSLLISNLTEQDQGTYRCSINNKQSIDIRLNIKGCTLSETKDKLIIRSPGDSVLLPCSCTDQQTKPRNVKWERLDSGGTEVLTTNNTGRVQMFNKNLPANLSLLISNLTQKDQGTYRCSINNNQSINITLNITEDTGETVKLESWTLIIIVCALLLILLLLLGGAACTYCKHGKGMNKCGCTNTHRKMYHTYAAFTQLMFKFHRKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAS1</accession>
    <name>A0A3B4EAS1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thyrotropin-releasing hormone receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thyroliberin receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor for thyrotropin-releasing hormone (TRH). Upon ligand binding, this G-protein-coupled receptor triggers activation of the phosphatidylinositol (IP3)-calcium-protein kinase C (PKC) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576845.2">
        <property type="nucleotide sequence ID" value="XM_017721356.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EAS1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032925"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037626.2">
        <property type="protein sequence ID" value="ENSPNAP00000032925.2"/>
        <property type="gene ID" value="ENSPNAG00000021207.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441692"/>
    <dbReference type="CTD" id="569382"/>
    <dbReference type="GeneTree" id="ENSGT01150000286932"/>
    <dbReference type="OMA" id="TIITCGY"/>
    <dbReference type="OrthoDB" id="10036964at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004997">
        <property type="term" value="F:thyrotropin-releasing hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14995">
        <property type="entry name" value="7tmA_TRH-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002120">
        <property type="entry name" value="TRH_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46061">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46061:SF6">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00751">
        <property type="entry name" value="THYROLIBRINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01846">
        <property type="entry name" value="TRHRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="258"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="39"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032925.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="384" mass="42531" checksum="6E08DBEC15AAE177" modified="2024-11-27" version="2">MENFTSPNNTLGPWTDRSTAYKVISAFLVLTICALGIVGNVMVVLVVLTTKHMRTPTNCYLVSLAAADLMVLVAAGLPSVADSLYGSWLFGHIGCLCITYLQYLGINASSCSIAAFTVERYIAICHPIRAQSLCTYTRAKRIILGVWLTTSLYCVMWFYLADTEELVYEDTIVVACAYKVSRNLYLPIYFFDFGVFFVLPLVLSTVLYGLIARILFLNPLPKGKSKNGHDGLSAGSKMNGLKSSSQCSSTTAASRRQVTKMLAVVVILFAVLWMPYRTLVVVNSFLQEAYLDSWFLLFCRICVYLNSAVNPVIYNAMSQKFRAAFRRLCSCGPRAQAKQAAYSVALTYSVAKETSMIESTEHFSTEMDDLTEGPDDLTEEPMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EC24</accession>
    <name>A0A3B4EC24_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">trypsin</fullName>
            <ecNumber evidence="5">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EC24"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033248"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023948.2">
        <property type="protein sequence ID" value="ENSPNAP00000033248.2"/>
        <property type="gene ID" value="ENSPNAG00000021742.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144271"/>
    <dbReference type="OMA" id="MLSSKNC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000005">
        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF87">
        <property type="entry name" value="ARGININE ESTERASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043814551" description="trypsin" evidence="6">
        <location>
            <begin position="20"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="26"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033248.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="244" mass="27014" checksum="8778C214CC68A2FD" modified="2024-11-27" version="2">MALISLLLLAALLPQLGHSASVHVGIVNGKVAQSRPYMVSLQVRTQHFCGGFLVSEQFVMTAAHCKYKGKPWTDVTAVIGAHNLKTNSFQRIQLMDSYSHPDFKMDPKYPENDIMLLKLAKPVVNKKIRIPEKDEDFKENITCSIAGWGRIGNNGPTNDVLLEADVTMLSSKNCKNWWNFNNILCTDSHHGGFCAGDSGGPLVCNNTAVGVIAFYEKTCINPPRPYGYTKISAFLPWIKKIMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECP9</accession>
    <name>A0A3B4ECP9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017550676.1">
        <property type="nucleotide sequence ID" value="XM_017695187.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECP9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034317"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026047.2">
        <property type="protein sequence ID" value="ENSPNAP00000034317.1"/>
        <property type="gene ID" value="ENSPNAG00000023514.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426033"/>
    <dbReference type="CTD" id="100126139"/>
    <dbReference type="GeneTree" id="ENSGT00940000158108"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000021">
        <property type="entry name" value="Basic krueppel-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000563">
        <property type="entry name" value="Krueppel-like factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF56">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="67"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="209"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="368" mass="40429" checksum="490D73CA57A7765F" modified="2018-12-05" version="1">MMSCPEMKPSMNLSDNSINNRMLMLDGMPAVRVKAEPLESERGSPRVHSYPDMEAVPLFLSKVKAEPPEDLLSSEQFQTQTEPVDLSINKARPSPPSATGSPITSAFSPSSSSSSSSSSPSVIASVSSGMPSVLTPGPLVGSPPGVRGQPFLHIIHPVPPSSPSKLASQVHRIPVVVQSLPLMYTTAVRSPSNHLNSTIMLPLVDEAKGKAQTDPNGLSPRQIKSDSDDDDLPNVTLDSVNETGSTALSIARAVQDSMSPFSIESMRRPRRSESPDSKKRRIHRCDFEGCNKVYTKSSHLKAHRRTHTGEKPYKCTWDGCTWKFARSDELTRHYRKHTGVKPFKCADCDRSFSRSDHLALHRRRHMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECT0</accession>
    <name>A0A3B4ECT0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cystatin fetuin-B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017573358.1">
        <property type="nucleotide sequence ID" value="XM_017717869.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECT0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033645"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038068.2">
        <property type="protein sequence ID" value="ENSPNAP00000033645.1"/>
        <property type="gene ID" value="ENSPNAG00000022415.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439441"/>
    <dbReference type="GeneTree" id="ENSGT00950000182930"/>
    <dbReference type="OMA" id="NCQFAHR"/>
    <dbReference type="OrthoDB" id="9941887at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025764">
        <property type="entry name" value="Cystatin_Fetuin_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050735">
        <property type="entry name" value="Kininogen_Fetuin_HRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814">
        <property type="entry name" value="FETUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814:SF17">
        <property type="entry name" value="FETUIN-B PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51530">
        <property type="entry name" value="CYSTATIN_FETUIN_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017231338" description="Cystatin fetuin-B-type domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin fetuin-B-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin fetuin-B-type" evidence="6">
        <location>
            <begin position="137"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="319" mass="35241" checksum="05A70BC37D250193" modified="2018-12-05" version="1">MLKYVFLLFICLHLQNGIATPDPTGCQDPAIVTAAEETLDHINAYRQEGYIFSLNRIYDVKQEPKEGGASLLHLIIDVLETKCHVISKKAWKSCEVKTVSDVPVFGKCEASVTFQTTIILHNFNCTIQQVPAVAIVETCPDCPTAERLDEPIIVETANLSMQKFNKETTMPNYFALLNITSASMQWVVGPAYFVEFTIQETDCAKASTDVNFTQCRPKDGPSIKGFCTGSHTTIDDSPEIKIPIEVNCSMYKPTPDNEPEKFHDAISHRTTEKPAVQTPSGSVLLLPPPPIPKPPRVRAAASNCPGERRHSLGLRALKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EH13</accession>
    <name>A0A3B4EH13_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoplasmic reticulum membrane protein complex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ER membrane protein complex subunit 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">EMC7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017581273.1">
        <property type="nucleotide sequence ID" value="XM_017725784.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EH13"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034589"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038633.2">
        <property type="protein sequence ID" value="ENSPNAP00000034589.1"/>
        <property type="gene ID" value="ENSPNAG00000023972.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444566"/>
    <dbReference type="CTD" id="561549"/>
    <dbReference type="GeneTree" id="ENSGT00390000017490"/>
    <dbReference type="OMA" id="EMENMQM"/>
    <dbReference type="OrthoDB" id="27095at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019008">
        <property type="entry name" value="Beta_sandwich_EMC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039163">
        <property type="entry name" value="EMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09430">
        <property type="entry name" value="EMC7_beta-sandw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="12">
        <location>
            <begin position="54"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="237" mass="26766" checksum="24F5C0ABDB6782B4" modified="2018-12-05" version="1">MQHLRRLLDAYFVLQSVFVLSRCFSEPEPGPSAASPASGDRFKIEGRAIVPGFKPQEWVSSARVLVEGGEYVGFLRTDGSFAVYDIPSGSYVIEIVSPSFRFEPTRVDITSKGKMRSRLVNYIKTSEVIRQPYPLQMRSSGPHTYFMKRETWGWTDFLMNPMVMMMVLPLLIIVLLPKVVNTNDPEMRKEMEQSMNMLNPNPELPDVSEFMTKLFSKGSSKPGGGSKGSRNAALKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EIU1</accession>
    <name>A0A3B4EIU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">V-set and transmembrane domain-containing protein 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">VSTM2L</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EIU1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030795.2">
        <property type="protein sequence ID" value="ENSPNAP00000036357.1"/>
        <property type="gene ID" value="ENSPNAG00000004173.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159381"/>
    <dbReference type="OMA" id="WANKETW"/>
    <dbReference type="OrthoDB" id="9870402at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000735">
        <property type="entry name" value="V-set and transmembrane domain containing 2 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF31">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="34"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="212" mass="23461" checksum="9B3E8952D88C1860" modified="2018-12-05" version="1">MGAFGVILGSLHYMGLYIQLSGQADVENRISSNAFFTEVPHDITAQSGQDVEMACSFRGAGSPSYSLEIQWWYIRNHKEWTDKQTWTTNQVVPQDELSKDATKISVVKVAGSNISHKLRLSSVKPSDEGTYECRVIDFSDSKLRHHRVRAYLQVEQAGGKPTAPHHQGAAAASPAQEPHKHQPAHGHHNPGRELRKRSADDSTSDCTESCVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJ51</accession>
    <name>A0A3B4EJ51_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017556591.1">
        <property type="nucleotide sequence ID" value="XM_017701102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJ51"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035246"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027940.2">
        <property type="protein sequence ID" value="ENSPNAP00000035246.1"/>
        <property type="gene ID" value="ENSPNAG00000025054.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429397"/>
    <dbReference type="CTD" id="100321746"/>
    <dbReference type="GeneTree" id="ENSGT00940000157859"/>
    <dbReference type="OMA" id="HCEQFAK"/>
    <dbReference type="OrthoDB" id="435273at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF45">
        <property type="entry name" value="CALMODULIN LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="153" mass="17605" checksum="B94C50CC8EDA38D6" modified="2018-12-05" version="1">MAKFLSHDQINEFKECFSLYDKNHKGRIKAHDLIVVMRCLGCSPTLTEVDRHLKMHNIDRSGELGFSTFLIMMHEQLKQESPEEEIMKALRVMDKQKNGFVLASDLRAKLTALGEKLTDQEVDELFREAGVGNDGCIYYEEFAKMVTLQSTRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKG5</accession>
    <name>A0A3B4EKG5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NFU1 iron-sulfur cluster scaffold homolog, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580976.1">
        <property type="nucleotide sequence ID" value="XM_017725487.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EKG5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030591.2">
        <property type="protein sequence ID" value="ENSPNAP00000036273.2"/>
        <property type="gene ID" value="ENSPNAG00000026998.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444338"/>
    <dbReference type="GeneTree" id="ENSGT00940000166680"/>
    <dbReference type="OMA" id="MAAHVRW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF45">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="3">
        <location>
            <begin position="59"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036273.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="260" mass="29064" checksum="5CAF2376BC5FC03E" modified="2024-11-27" version="2">MAAAVRWNLLRVLRSQAASPPIRFSLKDRSQCYSLWLAQKHRILQQSENKQILVRCLSILTQDTPNPRSLKFLPGKPVLGTGTLDFPTSSSAECSSLAKNLFQVEGVKSVFFGPDFITLTKIDDVEWMDIKDNAVEVITKFFESGEPVTTGVTHPENSVSEEDDDIVSMIKELLDTRIRPTVQEDGGDVIFKGFEDGTVKLKLVGSCTGCPSSTVTLRNGIQNMLQFYIPEVDDVEQVEDEVDEINMKVFTEVEQKLKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EKH2</accession>
    <name>A0A3B4EKH2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="27">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="35 36">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="35 36" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="27">Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="27"/>
    <dbReference type="RefSeq" id="XP_017574800.1">
        <property type="nucleotide sequence ID" value="XM_017719311.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000036952"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040408.2">
        <property type="protein sequence ID" value="ENSPNAP00000036952.1"/>
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        </source>
    </evidence>
    <sequence length="1424" mass="158489" checksum="41D1AD3626B0AAC2" modified="2018-12-05" version="1">MMMMMMMMMMMVRRRRRLLLHWLLLAAVAVGMGASQAVCPGTDNKLSTLSDLDQQYHTLRKFYENCEVVMGNLEITSIERNRNLNFLKSIREVTGYVLVALNQFDYLPLENLRIIRGTKLYEGRYALAIFLNYRRDGYFGLRQLGLKNLTEILNGGVYVDQNKFLCHADTIHWQDIVKNPRAELLVVPSNNSGSSCRRCHRSCNGRCWGHQEDQCQTLTKTVCAEQCDGRCFGPYVSDCCHRECAGGCSGPKDTDCFACTNFNDSGACVTQCPQPFVYNPTTFQLEHNPKAKYTYGAFCVKKCPHNFVVDHSSCVRACPSNKMEVEENRIKMCIPCTDICPKVCDGIGTGSLQMAQTVDSSNIDKFVNCTKINGNLIFLITGIKGDMYHGIGALDPERLNVFRTVKEITGFLNIQSWPENMTDLGVFSNLATIGGRSLYSGISLLILKQRWITSLQFQSLQEISAGNVYITNNSQLCYYNTVNWTGLFRTSGQRALIKSNKDPRECTHERMVCDKLCSEAGCWGPGPDQCLSCRFYSRGRTCVKSCSLYSGEVREFVNGSVCVECDSQCERAGDKTLTCHGPGPDHCTKCLHLKDGPNCVEKCPDGLQGANSFIFKYAESNNECHPCHPNCTQGCTGPRIQDCIGMLDRTPLIAAGVIGGMFVAVIVALGFAIYVRRKSIKKKRALRRFLETELVEPLTPSGTAPNQAQLRILKETELKRVKILGTGAFGTVYKGIWVPEGESVKIPVAIKILNETTGPKANVEFMDEALIMASMEHPHLVRLLGVCLSPTIQLVTQLMPHGCLLDYVHEHKDNIGSQLLLNWCVQIAKGMMYLEERRLVHRDLAARNVLVKSPNHIKITDFGLARLLDADEKEYNADGGKMPIKWMALECIHYRKFTHQSDVWSYGVTIWELMTFGGKPYDGIPTREIPDILEKGERLPQPPICTIDVYMVMVKCWMIDADSRPRFKELAAEFSRMARDPQRYLVIQGDDCMKLPSPNHSKFFQSLLDEDDLGDLMDAEEYLVPQSVNASASSYPPRLHLDSNRNQCGYRDGAIPPEEIAGAAVSSGSPSNPARTQRMPLDSQEEKHCNGSLKKQTGPGLVEDSCGQRYSADPTVFLGQITQRGGTDEDGYVAPRKDKSSLEHLNPIEENPFVTRRKNREIHALDNPGYHITPDGQPNVEDEYINEPLYHNTGEKSQETLKKNGIPVPMLSLTATNTTNSHALLTTQTGRPHHHSQGAHVHFAIPPVQPPQSGPMSQNGHHAHSVKGLSTDHPSKSGHPSATGQICHISHQVQPGYTGPGSLSDQQAQMAKSAATAKSSITGLPGHVAHQSSMPQASHTGTIMVDKTYKNTFDNPEYWHHSLPSKVAQHPQDPSLICSNNFLYKQNSRVRSGIAENPEYLSESGMKPGTVLPPPPYRQRNTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IGQ8</accession>
    <name>A0AAR2IGQ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane-spanning 4-domains subfamily A member 4A-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548525.1">
        <property type="nucleotide sequence ID" value="XM_017693036.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392292.1">
        <property type="nucleotide sequence ID" value="XM_037536395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IGQ8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047009.1">
        <property type="protein sequence ID" value="ENSPNAP00000038168.1"/>
        <property type="gene ID" value="ENSPNAG00000031628.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108424800"/>
    <dbReference type="GeneTree" id="ENSGT00940000163727"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF128">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25270" checksum="27864147E5264053" modified="2024-11-27" version="1">MSATAVPLSNARNGYTIVTQVISPPTSTQPGQMIYAVGPLRKFLKGKPKLFGTLQIMIGMLKFLLGFMMFFNAFSLTVIIGIAFWCALMYIITGSLAVSASTNIHRCVVTGALVMNVISAITATITITVVSLFLAFVFRPCYYGCPTSPNFVIGILLVISSIIQFIISIYISAFACKATCSDEPSVNITVAPNQAGCPDYPGEQYLMYAVNGATMNSPPMERPPPYNEIKDNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IQ03</accession>
    <name>A0AAR2IQ03_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-X-C chemokine receptor type 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Interferon-inducible protein 10 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CXCR3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IQ03"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066972.1">
        <property type="protein sequence ID" value="ENSPNAP00000039726.1"/>
        <property type="gene ID" value="ENSPNAG00000030515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244848"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016494">
        <property type="term" value="F:C-X-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002685">
        <property type="term" value="P:regulation of leukocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15180">
        <property type="entry name" value="7tmA_CXCR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000159">
        <property type="entry name" value="C-X-C chemokine receptor type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004070">
        <property type="entry name" value="Chemokine_CXCR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF671">
        <property type="entry name" value="C-X-C CHEMOKINE RECEPTOR TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01532">
        <property type="entry name" value="CXCCHMKINER3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0765">Sulfation</keyword>
    <keyword evidence="14 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="65"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="365" mass="41242" checksum="746CBBBA6BAB0E37" modified="2024-11-27" version="1">MDSGEKITLYYPDFNFLEENDTYNESYYDDGSCCGTVCGQEASMAFEAIFIPILYSLAVVIGLLGNGLVLVVLWQKKRAWSVTDTFVLHLSVADILLLLTMPLWAVEAANEWIFGTELCKLTGALFQVNFYCGIFLLACISLDRYLSIVHAVQMYSRKKPWLIQLSCMAVWFFSLLLSIPDWVYLGATEDSRRGKTECVHWYPSDGWRLASRLLYHIVGFVIPALVLVFCYTCILLKLQSGSQSLQKQRAMRVILALVVAFFVCWTPYNITLMVDTIHTNKSNQSSNVCDTTTALDIAITTTSTLGYLHCCMNPVLYAFVGVKFRRHLLDMARPISSRLKGRVNTVSRKTSMWSESVDTSHTSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IXH3</accession>
    <name>A0AAR2IXH3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone H3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IXH3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071927.1">
        <property type="protein sequence ID" value="ENSPNAP00000042752.1"/>
        <property type="gene ID" value="ENSPNAG00000030754.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286903"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000047">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="4">
        <location>
            <begin position="44"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="103" mass="11803" checksum="5DA18396B541E6BC" modified="2024-11-27" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQRLTSCRKITQKALLRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J078</accession>
    <name>A0AAR2J078_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Circadian associated repressor of transcription a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J078"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074224.1">
        <property type="protein sequence ID" value="ENSPNAP00000045393.1"/>
        <property type="gene ID" value="ENSPNAG00000032278.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164936"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031373">
        <property type="entry name" value="Ciart"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35441">
        <property type="entry name" value="CIRCADIAN-ASSOCIATED TRANSCRIPTIONAL REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35441:SF1">
        <property type="entry name" value="CIRCADIAN-ASSOCIATED TRANSCRIPTIONAL REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15673">
        <property type="entry name" value="Ciart"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="35227" checksum="03B24C237F8B5F57" modified="2024-11-27" version="1">MQSLGSSSEWPSKAALSSTTRLVFSESDQTEDDSSSEASSEGVVEAGLGKPSPAPFTTELPQSPEPRSGCGDQRGSTVESDQTGLCHRSPAYAGLTSVSAPRAPTEGDLAFSRKCAELSGYVRPLLDLLNGLKTGRYDKGLSTFQQSVAMDRLQRIAGILQKPHLGEKYLRTLLQLEMMLKVWFPQVVSCNPNSTPLSQSSTASTQPRWHQNQLPVKKRRLSWSDSESQSPAPPNCKLSRDKEPEQSPSLTTSSTHPEDTVSRSRGPVISDSEGGSCSDTQDCSVSSTTTTSDPCQSTGRQEAAVPLHSHDQVQGKWLNFCNCTSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0U3</accession>
    <name>A0AAR2J0U3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">BACH1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0U3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086248.1">
        <property type="protein sequence ID" value="ENSPNAP00000043879.1"/>
        <property type="gene ID" value="ENSPNAG00000035464.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158923"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF23">
        <property type="entry name" value="TRANSCRIPTION REGULATOR PROTEIN BACH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="179" mass="20643" checksum="B8D3AADE9C820A26" modified="2024-11-27" version="1">MSVDGPRTSVFTFQSAVHTVHVLRSLDEQRQKDVLCDVTVEVGCRSFRAHCSVLACCSDYFYTRLLNHTGWNFVITLPDEVTVEGFVPLLQFAYTAKLHFTKENILEIHRCAELLGFHNLDKACFEFLIPRFTDGSRTTQEAKKKSKNSNKSKPCHEEQTYWTHARVLHLVHIVWTNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J5R4</accession>
    <name>A0AAR2J5R4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037401732.1">
        <property type="nucleotide sequence ID" value="XM_037545835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J5R4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043666.1">
        <property type="protein sequence ID" value="ENSPNAP00000047363.1"/>
        <property type="gene ID" value="ENSPNAG00000036879.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108416575"/>
    <dbReference type="GeneTree" id="ENSGT01050000244840"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015057">
        <property type="term" value="F:thrombin-activated receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000040">
        <property type="entry name" value="Coagulation factor 2 (thrombin) receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002281">
        <property type="entry name" value="Pro_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003912">
        <property type="entry name" value="Protea_act_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF21">
        <property type="entry name" value="PROTEINASE-ACTIVATED RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01428">
        <property type="entry name" value="PROTEASEAR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01152">
        <property type="entry name" value="PROTEASEAR2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="68"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="360"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="124"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="403" mass="45337" checksum="DC6A95DECCE0796E" modified="2024-11-27" version="1">MDPASTAECVYVPSDNQRSWIYYGFDVVEENAEGVKVSNASGPALKGDLTTVFLPAVYIIVFIVGLPTNAMALWVFLFRTKKKHPASILMANLALADLLFIVWLPLKITYHFNGNDWTFGEPLCKVLVGFFYGNMYCSAIFIACISVQRYWGIVHPLSQKLNNRVTVCVCVCVWIVVWVLTVPLYLYDQTVKVTNMCITTCHDVTRFNQTHFPVGYFLTMGTVGYVVPCVVCIVSYLLTFLSLRRSVTDSSSSKKKRKAIVLMVTVLVMFLVCFTPSNIMLMVHYSLLGAKIPNNVYGIYMVALCLGSLNSCLDPFVYYYISEEFRDHVKNSLMCRSERTSKQMKVSFSALKFSKKSNTYTSDSAHTQSRDCSSDSAHIQSRDCSSDSAHTQRRDCSSDSTHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J7S8</accession>
    <name>A0AAR2J7S8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">OTUB2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C65 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J7S8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044943.1">
        <property type="protein sequence ID" value="ENSPNAP00000046377.1"/>
        <property type="gene ID" value="ENSPNAG00000034823.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108423522"/>
    <dbReference type="GeneTree" id="ENSGT00390000006979"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000780">
        <property type="term" value="P:negative regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1300.20:FF:000001">
        <property type="entry name" value="Ubiquitin thioesterase OTUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.60">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.20">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042468">
        <property type="entry name" value="Peptidase_C65_otubain_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042467">
        <property type="entry name" value="Peptidase_C65_otubain_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="8">
        <location>
            <begin position="34"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="227" mass="26041" checksum="C9A22D277F1BAEF2" modified="2024-11-27" version="1">MDESGLVSLKEATSAQVLEEPEATKCQDICHQFGFIRRVKGDGNCFYRAFCFRLVETLVHNESAMKRIKDKLLRSHEELLKAGFDENEFKDLLSTFLRVLDQLEADDREETLLGFFNDQATSDSMVKYLRLLTSAHLQNHADFFQHFVEAPSLKAYCTQEVEAMAMECDHVEILALSETLDISVCIISMDGSNSHPVYHTIPEGSQPSLYLLYKTSHYDILYKCREH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J856</accession>
    <name>A0AAR2J856_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J856"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043044.1">
        <property type="protein sequence ID" value="ENSPNAP00000048213.1"/>
        <property type="gene ID" value="ENSPNAG00000035826.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155092"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF28">
        <property type="entry name" value="ZINC FINGER PROTEIN 37-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="101" mass="11743" checksum="145874BAF710E74B" modified="2024-11-27" version="1">MEFPDHSRQLLQCLSQQRHQGFLCDCTVLVGEARFKAHRAVLASCSMYFHLFYRDQLDKRDVVHLNSDIVTAPAFGLLLEFMYEGKLEFSTLPRSLFENLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J860</accession>
    <name>A0AAR2J860_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Guanylate kinase</fullName>
            <ecNumber evidence="4">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GMP + ATP = GDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer. Interacts with RD3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017542529.1">
        <property type="nucleotide sequence ID" value="XM_017687040.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J860"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082224.1">
        <property type="protein sequence ID" value="ENSPNAP00000048218.1"/>
        <property type="gene ID" value="ENSPNAG00000007280.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414219"/>
    <dbReference type="CTD" id="415216"/>
    <dbReference type="GeneTree" id="ENSGT00940000155815"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000879">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF23">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="14">
        <location>
            <begin position="16"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="210" mass="23229" checksum="9BD7C20EC5E01309" modified="2024-11-27" version="1">MFGRLLCRALSVMAGPRPVVLSGPSGAGKSTLLKMLMKEFNGLFGFSVSHTTRKPRPGEVNGKDYHYVSREVMQAGISNGEFIEHAEFSGNLYGTSKAAVQEVQARNLICILDIEMQGVKSIKKTDLNPIYISIQPPSMDILEKRLRDRKTESEESLQKRLRAASVDLEISKEPGLFDVVIVNDNLDDAYRELKGALIEEIQTVQGSTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDJ2</accession>
    <name>A0AAR2JDJ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zona pellucida sperm-binding protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Zona pellucida glycoprotein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Zona pellucida protein B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Zona pellucida</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JDJ2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079507.1">
        <property type="protein sequence ID" value="ENSPNAP00000050098.1"/>
        <property type="gene ID" value="ENSPNAG00000030425.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163253"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060468">
        <property type="term" value="P:prevention of polyspermy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017977">
        <property type="entry name" value="ZP_dom_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF31">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00682">
        <property type="entry name" value="ZP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0278">Fertilization</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="P-type" evidence="20">
        <location>
            <begin position="90"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="19">
        <location>
            <begin position="133"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="456" mass="49459" checksum="8F7A117733A482A9" modified="2024-11-27" version="1">SDKDIFLRLIACVALFVHLNSMGITAVSPWSPQAAKRPVPHFPGPRDPPQEAKWPLPHFPGPRDPPQEAKQPLPHFPGLLDPQQPVVTPHICGVDDVDRVPCGEPGIDAAQCGAINCCFDGQQCYYGRTVTVQCTIDGQFVLVVARDTTLPRLSLDSISLLGGSDAPCSAVDSNADFAIYQFPVTACGTRAMELGGYVVYENKMSSSYEVGVGPLGAITRDSHYELYFQCRYADATVAALAVQLASNNPPMPVAASGPLMVELRLGSGQCVTKAQAAYTSYYGDADYPVTKVLREPVYVEVRILGRTDPNIALVLGRCWATSSPDPFSIPQWELLVDGCPYQHDSYLTTPIPVDGSSGLQFPSHYKRFVLKMFAFVDHASMAPLQEKVFIHCSTSVCQLTAADSCEPRCSRQRRDVVAAGRKSSHQLTVVSSGEVIFTEKAETVPPELQGNPNLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JEQ7</accession>
    <name>A0AAR2JEQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Group XIIA secretory phospholipase A2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphatidylcholine 2-acylhydrolase 12A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PLA2G12A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564534.1">
        <property type="nucleotide sequence ID" value="XM_017709045.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JEQ7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059437.1">
        <property type="protein sequence ID" value="ENSPNAP00000050498.1"/>
        <property type="gene ID" value="ENSPNAG00000002908.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434125"/>
    <dbReference type="CTD" id="81579"/>
    <dbReference type="GeneTree" id="ENSGT00390000008798"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000004">
        <property type="entry name" value="Group XIIA secretory phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010711">
        <property type="entry name" value="PLA2G12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12824">
        <property type="entry name" value="GROUP XII SECRETORY PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12824:SF7">
        <property type="entry name" value="GROUP XIIA SECRETORY PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06951">
        <property type="entry name" value="PLA2G12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="199" mass="22272" checksum="B4B23DD40CC595D1" modified="2024-11-27" version="1">MYCVCSVGWAMQCGVTSAVLLLSIGFMTSGFEEFEPETPDWRKTLKTIRNGIHKIDTYLNYALDLIGGSDGLCQYKCSDGYKPVPRPGYKLSPPNGCGSPLFGFQFDFGIPAMTKCCNLHDRCYDKCGQEKHDCDERFQVCLETICRNVQMTLGLDQSVQACESAVTLLFDAVMHLGCKPYLDSQRASCICRYEEKTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JET6</accession>
    <name>A0AAR2JET6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CTDSP2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JET6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064959.1">
        <property type="protein sequence ID" value="ENSPNAP00000048852.1"/>
        <property type="gene ID" value="ENSPNAG00000035505.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240451"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000013">
        <property type="entry name" value="Carboxy-terminal domain RNA polymerase II polypeptide A small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040078">
        <property type="entry name" value="RNA_Pol_CTD_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
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        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01124">
        <property type="entry name" value="C0.1:_RNA_Pol_CTD_Phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="9">
        <location>
            <begin position="98"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="6">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR640078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR640078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="272" mass="30591" checksum="C7CA89771ADEB1E6" modified="2024-11-27" version="1">MESSIITQVQKEDIQLPTKTGQVKKSSPKKPRSRNIFKALFCCLRAQDVPQPPPPSQDTLLPPEENGTIAKVSVLNTFCLFLSCQVPTASLLPEVTAEDQGKICVVIDLDETLVHSSFKPISNADFIVPVEIEGTTHQVYVLKRPHVDRFLQRMGEMFECVLFTASLAKYADPVTDLLDQSGVFRTRLFRESCVFHQGCYVKDLSRLGRQLNKTLILDNSPASYIFHPENAVPVLSWFDDLEDTELLNLLPVFEELSVAEDVCAKLKQLREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JIS7</accession>
    <name>A0AAR2JIS7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017580120.1">
        <property type="nucleotide sequence ID" value="XM_017724631.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JIS7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060095.1">
        <property type="protein sequence ID" value="ENSPNAP00000050104.1"/>
        <property type="gene ID" value="ENSPNAG00000034697.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108443775"/>
    <dbReference type="GeneTree" id="ENSGT00940000163520"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF102">
        <property type="entry name" value="CEREBELLIN 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043647301" description="C1q domain-containing protein" evidence="5">
        <location>
            <begin position="26"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="6">
        <location>
            <begin position="96"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="46"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="236" mass="26235" checksum="FBD8C7D1FAA16548" modified="2024-11-27" version="1">MERTAAVAVALVMLLQSSLQVAVKSQDCSDELKQLKDLVYQPLTALAELKVKVSYMEKENTALEARLASHEKEMENLKKENEAMKRDLEKLRIEISGMPKVAFSAGLPAGQMGPVNTESTLIYSKVLTNIGGAYNPYTGAFTAPVRGVYYIRFTAAAYNSDSNNMGLNLYKNDRHLMHLGEYNSDGKARHVSGALTLELEAGDVLYARLPENYMLYDDSLFRNSFSGFLIFPRSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLN4</accession>
    <name>A0AAR2JLN4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TFIIS central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLN4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055562.1">
        <property type="protein sequence ID" value="ENSPNAP00000051207.1"/>
        <property type="gene ID" value="ENSPNAG00000035258.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162067"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035100">
        <property type="entry name" value="TF_IIS-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR A N-TERMINAL AND CENTRAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006704">
        <property type="entry name" value="TF_IIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS central" evidence="1">
        <location>
            <begin position="20"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="173" mass="19406" checksum="635E368810460E15" modified="2024-11-27" version="1">MSSNYLVPPQPRSPTDSPALRRKCTELLLQALTPSQKTDPESTNQLSTIAQAIERHVHAVHGQNQVKYKSCIRSKVANLKNPKNPHLRQGLLTGTLAPEVFAQMSVEEMAGEELQRLREGYTSAAISEHQLPQRLEGTTTTKVRCKLPHEDAMTFLTCTGCGEQWYHSRWVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JUC3</accession>
    <name>A0AAR2JUC3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558326.1">
        <property type="nucleotide sequence ID" value="XM_017702837.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JUC3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076795.1">
        <property type="protein sequence ID" value="ENSPNAP00000055595.1"/>
        <property type="gene ID" value="ENSPNAG00000000320.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430373"/>
    <dbReference type="CTD" id="406541"/>
    <dbReference type="GeneTree" id="ENSGT00940000157207"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000028">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000003">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002167">
        <property type="entry name" value="GDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00928">
        <property type="entry name" value="GRAVESDC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="184"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="278"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="375"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="469" mass="52464" checksum="37772E9DD942A3CF" modified="2024-11-27" version="1">MLCLCLYVPVPNSDPVEVEYFESNGLPSELKSLFKLSVLLPSQEFSTYRKWRKKMAKTEEQDLNGQLDFEEFVHYLQDHEKDLKLVFRSLDKKNAGKASAQEIVESLRELGVHMSLQQAERVLKSMDKNGTMTIDWNEWKNYPSQQPTESIPEIILYWKHSTIFDVGENMMVPDEFTTEEHLTGMWWRHLVAGGGAGAVSRTCTAPLDRLKVLMQVHGSRSSNMCIMSGLTQMIKEGGVRSLWRGNGINVLKIAPESALKFMAYEQIKRLMGSSNETLGITERFVAGSLAGVIAQSTIYPMEVLKTRLALRKSGQYSGISDCARQILKKEGVAAFYKGYLPNMLGIVPYAGIDLAVYETLKNTWLQRYGKDSADPGVFVLLACGTVSSTCGQLASYPLALVRTRMQAQATVDGAPQITMSRLFKQIMKTEGPSGLYRGMTPNFMKVIPAVSISYVVYEHIKSTLGVQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K3L6</accession>
    <name>A0AAR2K3L6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/potassium-transporting ATPase subunit beta-1-interacting protein</fullName>
            <shortName evidence="7">Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NKAIN4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NKAIN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037397426.1">
        <property type="nucleotide sequence ID" value="XM_037541529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K3L6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061418.1">
        <property type="protein sequence ID" value="ENSPNAP00000057067.1"/>
        <property type="gene ID" value="ENSPNAG00000032110.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108410586"/>
    <dbReference type="GeneTree" id="ENSGT00940000157989"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008516">
        <property type="entry name" value="Na/K-Atpase_Interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084:SF5">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084">
        <property type="entry name" value="T-CELL LYMPHOMA BREAKPOINT-ASSOCIATED TARGET 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05640">
        <property type="entry name" value="NKAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043501752" description="Sodium/potassium-transporting ATPase subunit beta-1-interacting protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="208" mass="23870" checksum="DFF246F9A404BE59" modified="2024-11-27" version="1">MGCCTGRCSLIFLCTVQLMVAFERQVFDFLGYQWAPILANFFHIIVVILGLFGTIQYRPRYIVVYAVWTALWVAWNVFLICFYLEVGGLSKESDLLTFNISAHHSWWSEHGPGCVRREMPPSGMRNAETHSYVSVMGCLLEYQYIEVLHSGFQILLSLLGFVYACYVVSSITEEEDSFDFIGGFDPFPLYHVNEKPSHLLLKPMYQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K8Y9</accession>
    <name>A0AAR2K8Y9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRK SH3-binding GNRP</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017539440.1">
        <property type="nucleotide sequence ID" value="XM_017683951.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K8Y9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000085896.1">
        <property type="protein sequence ID" value="ENSPNAP00000058949.1"/>
        <property type="gene ID" value="ENSPNAG00000023888.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412075"/>
    <dbReference type="CTD" id="394101"/>
    <dbReference type="GeneTree" id="ENSGT00940000156235"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000009">
        <property type="entry name" value="rap guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF224">
        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="750"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="902"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="409"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="577"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="693"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="604"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00083313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1139" mass="127673" checksum="53B0C9C2B78C5293" modified="2024-11-27" version="1">MSGKIESKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKQHQPEPAVKSTEKPVNRKVSRLEEQEKEVVSALRYFKTIVDKMAVDSKVLQMLPGSASKVLEAILPLVQVEARIQHSSAIASCHNRVYQSLANLIRWSDQVMLEGIDLDDKDTVTTVTTVIKAVLDGVKELVKLTIEKQEQPSPTSPSKPVPPVAKTESECEMPLTEREKEILNKTTPIATPTDSLTDISEEEVAPPKPPLPGLKLAEHRSRMQLCSDPVAGQWRAAVMETPPALPPKKRQSAHSPTRVAIVAPMSRTNCGLNLPPGALKQQQEYEVDLHHRRFSGGSQSYGGDSPRLSPCSSMGKLSKSDEQLSSLERDSGQCSRNTSCETLDNTEGYDPDYDFLHQDLSVSEPLPFPTVHSGCLSPLPESLSESPSPGPTHPRFSAPLSSQSSLDHWTPPSTAGASNGEQPPALPQKKRRSVPILSSCPLYERLPSHYDNLQEEDTPTPPFPLVTPVSPGLQCNGSEAETPSVFSDSPPPLPEKKSKTILQYMQFVEDYSEPQPSVFYQTPQSERIYEQRRNKHFQEVYGIHESYSSSDSVHEPAPPPALPPKQRQLASHSSSPSSSSSSSLSLLPPSAGVPEEAESALGLSLSVSNSFLSGHASLTTPMSESANDEGGEGEYVNLYSATQANGDATHHSDAPAHCDDVVIQDPAPHIPPSNSKEALAKDRLKSTDSGHVKEDVDELTLVDHKEIMNRITLKTEGDDGPDVRAGSSDILLVHATETDRKDLVLYCEAFLTTYRTFITPEDLIKKLHYRYTHFCHSPDTFKKRVSKNTFFVLVRVVDELCLVELTEDILRQLMELVFRLVCNGELSLARVLRKNILDKVEQKRLLRHTHILKPLAARGVSARPGTLHDFRSHEIADQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIIQQEKAQDREKLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLEEYCTLIDSSSSFRAYRAALADVEPPCIPYLGLILQDLTFVHLGNPDLIDGKVNFSKRWQQFNILDSMRRFQQVHYELKRNEDIISFFNDFSDHLAEEALWELSLKIKPRNITRRKTEREEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KCG9</accession>
    <name>A0AAR2KCG9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KCG9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060133.1">
        <property type="protein sequence ID" value="ENSPNAP00000061965.1"/>
        <property type="gene ID" value="ENSPNAG00000035754.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000288772"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="183" mass="16791" checksum="D7E3EA80BE756D66" modified="2024-11-27" version="1">MPSPPTPSPDPGTPPAPSPPNPSPDPGTPPAPSPPNPSPDPGTPPAPSPPNPSPDPGTPPAPTLGMPPIPGIPPAPGTVPTPGIPPAPGTAPGTPPAPGTVPTPGTPPAAGTAPGTPPAPGTAPGTPPAPGTAPGTPPAPGTAPGTPPAPGTAPGIPPAPGTALTPGIPPTPSVPPPSRPRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHK3</accession>
    <name>A0AAR2KHK3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">KPNB1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family. Importin beta-1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHK3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047232.1">
        <property type="protein sequence ID" value="ENSPNAP00000061939.1"/>
        <property type="gene ID" value="ENSPNAG00000024047.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074898"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000027">
        <property type="entry name" value="Importin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="129"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="409"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="939" mass="104732" checksum="CE6DA1B99865786C" modified="2024-11-27" version="1">MELITILEKTVSPDRNELEAAQKFLEQAAIENLPTFLVELSKVLANPANTQVARVAAGLQVKNSLTSKDPDVKAQYQQRWLAIDGNARREIKNYVLQTLGTETYRPSSASQCVAGIACAEIPVRQWPELIPQLVANVTDPSSTEHMKESTLEAIGYICQDIDPEQLQDSANQILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKETERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEATEASEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDVVPHVLPFIKENIKHLDWRYRDASVMAFGSILEGPELNQLKPLVIQAMPTLIELMKDPSVVVRDTTAWTVGRICDLLPEAAINEIYLAPLLQCLIEGLGAEPRVASNVCWAFSSLAEAAYEATDAAEDQEEPSTYCLSSSFELIVQKLLETTDRPDGHQNNLRSAAYEALMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGSDFLKYMDAFKPFLVIGLKNYAEYQVDGFFLRALVLKDLGNDNVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLDTLQQASQAQVDKTDYDMVDYLNELREGCLEAYTGIIQGLKGDQENVHPDVMLVQPRVEFILSFIHHIAEDEDHSDGVVANAVGLIGDLCTTFGKDVMKMVEIRPQINDLLTEGRRSKTSKTKTLATWATKELRKLKSQAWWGAQSGSGILLTISQAEVCFLRLESRCCAFPCVIVWFLSTVLLLCFRSVADWGEPSRTLEEKRPSGVSCVFCTRVWEQKSSMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNX8</accession>
    <name>A0AAR2KNX8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LPXN</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017577819.1">
        <property type="nucleotide sequence ID" value="XM_017722330.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNX8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064834.1">
        <property type="protein sequence ID" value="ENSPNAP00000064122.1"/>
        <property type="gene ID" value="ENSPNAG00000037151.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108442341"/>
    <dbReference type="CTD" id="9404"/>
    <dbReference type="GeneTree" id="ENSGT00940000160259"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09336">
        <property type="entry name" value="LIM1_Paxillin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09410">
        <property type="entry name" value="LIM3_Leupaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09412">
        <property type="entry name" value="LIM4_Leupaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000008">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000009">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000012">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000018">
        <property type="entry name" value="Paxillin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047075">
        <property type="entry name" value="Paxillin_TGFB1I1_LIM_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF62">
        <property type="entry name" value="LEUPAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 11" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="173"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="233"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="291"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="351"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="15"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="408" mass="44363" checksum="999407F90C20E14A" modified="2024-11-27" version="1">MDELDMLLEELALTSSKSLPAPAPPQTLALSTSDKTATISVSAQGQSSGVGSGQGGAKNVYSELPAPVEAEVMSPGTASRELDSIMNELLGLGLEVPDEVPLSKPPPLAEKSEKEKKDGADGDKREGSNATNNVQSDKRLQRQMSKNIDAIDDLLGSLSSDMEKMGVRTAAKGHCASCGKCIAGKMITALGQVWHPEHFVCVVCKEELGTQGFFERDGKPYCETDYQNLFAPRCAYCKGPILQNILTAMDTTWHPEHFFCTHCGERFGTEGFLERDGKPYCPVDFYRLFAPKCSGCGEPVRENYLSAANGTWHPDCFVCADCLKPFTDGCFLEMDGRPLCSLHYHSRQGTLCGTCGTPISGRCIAALDRKFHPEHFVCAFCLRQLSQGVFKEQGGKPYCGPCHSKLFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KPB8</accession>
    <name>A0AAR2KPB8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">Solute carrier family 25 member 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">SLC25A14</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R,3R)-tartrate(in) + sulfate(out) = (2R,3R)-tartrate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73403"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30924"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(3R)-citramalate(in) + sulfate(out) = (3R)-citramalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73227"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(3S)-citramalate(in) + sulfate(out) = (3S)-citramalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73223"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30936"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(S)-malate(in) + sulfate(out) = (S)-malate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:71615"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(S)-malate(out) = (S)-malate(in)</text>
            <dbReference type="Rhea" id="RHEA:74555"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(S,S)-tartrate(in) + sulfate(out) = (S,S)-tartrate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73407"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30927"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-aspartate(in) + sulfate(out) = D-aspartate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73399"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-aspartate(in) + sulfate(out) = L-aspartate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73395"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>maleate(in) + sulfate(out) = maleate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73199"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30780"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>malonate(in) + sulfate(out) = malonate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73195"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>oxalate(in) + sulfate(out) = oxalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:72275"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>phosphate(in) = phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:32823"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sulfate(in) = sulfate(out)</text>
            <dbReference type="Rhea" id="RHEA:34983"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>sulfate(out) + phosphate(in) = sulfate(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:71631"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>sulfate(out) + succinate(in) = sulfate(in) + succinate(out)</text>
            <dbReference type="Rhea" id="RHEA:73411"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>sulfite(in) + sulfate(out) = sulfite(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73207"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>thiosulfate(in) + sulfate(out) = thiosulfate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73215"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 28">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KPB8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047896.1">
        <property type="protein sequence ID" value="ENSPNAP00000066173.1"/>
        <property type="gene ID" value="ENSPNAG00000015703.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165884"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000006">
        <property type="entry name" value="brain mitochondrial carrier protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00784">
        <property type="entry name" value="MTUNCOUPLING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 27 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 28" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="27">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="27">
        <location>
            <begin position="110"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="27">
        <location>
            <begin position="204"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00050259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="31">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="32852" checksum="FD533ECD72A5F328" modified="2024-11-27" version="1">MQLTGSATEMANLNWKPFVYGGMASIVAEFGTFPIDLTKTRLQVQGQSQCMEVRYRGMFHALLRIGQEEGIRALYSGISPALLRQASYGTIKIGTYNTLKKLFVSRPEDETMVINVFCGVVSGVLSSTLANPTDVLKIRMQAQGSLLQGSMMANFINIYQTEGTRGLWRGVIPTAQRAAIVVGVELPVYDITKKHLIRSGLMGDTVLTHFISSFACGLAGALASNPVDVVRTRMMNQRVLSGNFLYKGTLDGLMQTWRNEGFFALYKGFWPNWLRLGPWNIIFFITFEQLKKLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KSC4</accession>
    <name>A0AAR2KSC4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KSC4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074698.1">
        <property type="protein sequence ID" value="ENSPNAP00000065394.1"/>
        <property type="gene ID" value="ENSPNAG00000030417.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204454"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="354"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="60"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="157"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="259"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="460" mass="51712" checksum="3566715503889869" modified="2024-11-27" version="1">MFNQNHSRNFSLLISDLTKEDSGLYTCRADEKDMQSVQLEVTGCTLSENKQTVPVSRSSGESVLLSCTCTDQQDRPVGVQWRTPNREDLLQRYSGRVQTFNQSSTGKFSVLISDLTEEDGGTYSCWINQNQYRNFSLTVKGCTLSETEEKLIIRSPGDSVLLPCSCTDQYTKPVSVKWERVDSGGTEVSNEMELYTSRVQMFNKNLPANLSLLISNLTEQDQGTYRCSINNKQSINITLNINGCTLSETQEKLLIRSPGQSVLLPCSCTDQHTKPVSVKWGRVDSGGIEVSNEMANYTGRVQMFNKNLPANLSLLISNLTEQDQGTYRCSINNQQSINIRLSIEAEKREITGSFVIILCVCVTLLFIVVIGGSALIFYKRRNNKTQESVYINTERRTQNTDDGDSINYPSRNWKEISMPPINQCLDSGTDQSDSVYQNMTFNTNQSYLNPYTIKCQSTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KWX5</accession>
    <name>A0AAR2KWX5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KWX5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069292.1">
        <property type="protein sequence ID" value="ENSPNAP00000066646.1"/>
        <property type="gene ID" value="ENSPNAG00000035029.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267181"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="11">
        <location>
            <begin position="9"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="110" mass="12205" checksum="7F455E4A64022DAA" modified="2025-10-08" version="2">MTVTKTAGKGGKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGESSRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KXQ6</accession>
    <name>A0AAR2KXQ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Neurocan core protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Chondroitin sulfate proteoglycan 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000075782.1">
        <property type="protein sequence ID" value="ENSPNAP00000069133.1"/>
        <property type="gene ID" value="ENSPNAG00000020159.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158649"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03517">
        <property type="entry name" value="Link_domain_CSPGs_modules_1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03520">
        <property type="entry name" value="Link_domain_CSPGs_modules_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000011">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000571">
        <property type="entry name" value="Neurocan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000537">
        <property type="entry name" value="Notch 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF24">
        <property type="entry name" value="NEUROCAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044006334" description="Neurocan core protein" evidence="21">
        <location>
            <begin position="31"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="54"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="26">
        <location>
            <begin position="158"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="26">
        <location>
            <begin position="259"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="998"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1036"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="23">
        <location>
            <begin position="1085"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="25">
        <location>
            <begin position="1203"/>
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            <begin position="1266"/>
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        <location>
            <begin position="456"/>
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            <dbReference type="ARBA" id="ARBA00023319"/>
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    <evidence type="ECO:0000256" key="20">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
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    <sequence length="1302" mass="143018" checksum="43E9E96F5C6C83F2" modified="2024-11-27" version="1">MMLGEWRVAGLQALLTVCLLLFLEHDTVWGDTVVNMRKVTHRAVQVPLSGMALLPCVFTLRPSPSHDAPRIKWTKVWGQRGLDGLQREQSILVAKGNVVKVKKAFQGRVTLPGYSDNRYNASLALTGLRSSDSGMYRCEVVVGINDEQDTVPLQVTGVVFHYRAPHDRYALSFPEAKRVCVENSAVIATPGQLQATFDDGYDNCDAGWLSDQTVRYPIQSPRPGCYGDREDSPGVRNYGSRAPDELFDVYCFAESLQGEVFYTNVPEKLSLATASTHCHTLGAQLATVGQLFLAWQAGLDRCDPGWLADGSVRYPINQPRRNCGGDEPGVRTLYHNPNRTGFPETTNLFDAYCYQETEAKALLQTSDAFSSNSTDDWEQLQQNGAFPQPSTWTGLVDLDKEEFNSIADNDSSEISEEHVVIHLQPGERSVHWGEDATKDGSPPALENQVDLQGGSAREEHDEREATEGHSLSPDPSLTTPSSSTQAQTSNSVLYNFVNSIMKPLRYWTGNGETEASSTESSLVKETSEVVKPARMKPSGGLTESKGSTDNSILEPKQAEPSTNSSTLSSEEGLLEQEKEVVLTVPGHDLKNQSKAQTEPSISQAVPFNGVIASELMEAANPTAAASPLGSVSSFGSVPVLNSQQGWALKSLKGHGPSGNSGLLMAQTSGPMEAKRGALEIMAIPVSQDNHENFSGEGRDLIEESYPLPEGPPSQTSPQSQNTEEEKDTDGSGGKDLFNTSQIKDSLPSEDQSVILRTTVQWKLVDPSAGPTGMPPSGASISGPKQNDSDSTAAEEARGEILYMRRPTEITRNDPFNKVRVPLVKRKQNLNALVTSETQPPFAATTLELSPEATRATDVSSSVTMTTINNQIPTTDATTTRDFASTTDPIMFTWLPVVQEETHDLQTHLPTTTVTVVGFETMSTTLQLTTPDAKETSQPAESRSGVSESFVVDHGWTTPGQDSLPAESQPSTTTGKVSGDNSSEAVVDFGSTPTVALVEKDPCQLNPCLHGGSCLRDSDGYSCLCPQGYSGESCEIDIDDCQSSPCQNGGTCIDEINSFVCLCLPSYGGAMCEKDTEGCDHTWRKFHGHCYRYFTHRRTWEDAEKDCREHNGHLASIHTNEEQDFINSMSHENTWIGLNDRTVEEDFQWTDNMDLQYENWRENQPDNFFAGGEDCVVMIAHENGKWNDVPCNYNLPYICKKGTVLCGNPPLVDNAFLIGRKRSHYDIHSVVRYQCADGFLQRHIPTAKCRANGKWDHPKIICTRPRRSHRYRRHHHKSHHERRKHKKHGSGGHRGGQEGHGHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L2D3</accession>
    <name>A0AAR2L2D3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Unconventional myosin-Ic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Myosin I beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Stereocilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L2D3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076537.1">
        <property type="protein sequence ID" value="ENSPNAP00000070773.1"/>
        <property type="gene ID" value="ENSPNAG00000009340.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157915"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060171">
        <property type="term" value="C:stereocilium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000004">
        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000013">
        <property type="entry name" value="unconventional myosin-Ic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF255">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16 21" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="15 21" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13 21" id="KW-0518">Myosin</keyword>
    <keyword evidence="10 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="22">
        <location>
            <begin position="73"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="23">
        <location>
            <begin position="892"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="21">
        <location>
            <begin position="633"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="166"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00068083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1070" mass="121996" checksum="CC473CECE587E66C" modified="2024-11-27" version="1">LSLSAVSLSVSCHSLSPVSCLSLSCLSLSLSPVCLSLSISPLSLSPVSLSCAVGSDGVRVMMESALTARDRVGVQDFVLLENHTSEVAFIENLRKRFKENLIYTYIGSVLVSVNPYKDLEIYTKQHMERYRGVNFYEVSPHIYAVSDNAYRSMRTERRDQCMLISGESGAGKTEASKKVLQYYAVTCPASEQVQTVKDRLLQSNPVLEAFGNAKTLRNDNSSRFGKYMDIQFDFRGAPVGGHILNYLLEKSRVVHQSHGERNFHIFYQLIEGGEEDLLRRLGLERNTQQYQYLVKGNCPKVSSINDRSDWKLVRKALSVIGFSDDDVEELLNIIASVLHLGNVQFGGEDSGNAYITTDTQIKYLARLLGVDGSVLKEALTHKKITAKGEELMSPLNQEQASSARDALSKAIYGRTFTWLVNKINDSLAFKDDSFSSKNASVIGLLDIYGFEVFQNNSFEQFCINYCNEKLQQLFIELTLKSEQEEYEAEGITWEPVQYFNNKIICDLVEEKFKGIISILDEECLRPGDASDITFLEKLEDTVGGHVHFLSHKLADGKTRKVMGRDEFRLLHYAGEVNYSVNGFLDKNNDLLFRNLKEVMCMSENKILTQCFDREELTDRKRPETTATQFKNSLAKLMEILMSKEPSYVRCIKPNDAKQAGRFDEVLIRHQVKYLGLMENLRVRRAGFAYRRRYEVFLQRYKSLCPDTWPNWDGRLVDGVSTLVKHLGYKPEEYKLGRSKIFIRFPKTLFATEDALEEIAQPFVYRSAAILIQAWWRGILARRRAQRRREAADTIRRFIKGFMYRHQPRCPENEYFLDYVRYSFLMKLHRNLPKTVLDKSWPTPPPALIETSEHLRKLCMQNMVWKYCKTISPEWKHQLEQKMVASEIFKDKKDNYPQSVPKLFVGTRLNGEDINPKVLQALGTEKMKYGVPVTKYDRKGYKARARQMLLTGSSVFIVEDTKLKQRIDYSALKGISVSSLSDGMFVLHVPCEDNKQKGDVVLQSDHVIETLTKVAICADKINSININQGSIKFTVSQGKEGIIDFTSGSELLIAKAKNGHLSVTAPRLNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L538</accession>
    <name>A0AAR2L538_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tight junction protein 2a (zona occludens 2)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555730.1">
        <property type="nucleotide sequence ID" value="XM_017700241.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L538"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050468.1">
        <property type="protein sequence ID" value="ENSPNAP00000069767.1"/>
        <property type="gene ID" value="ENSPNAG00000026370.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428881"/>
    <dbReference type="CTD" id="565446"/>
    <dbReference type="GeneTree" id="ENSGT00940000158634"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090557">
        <property type="term" value="P:establishment of endothelial intestinal barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905605">
        <property type="term" value="P:positive regulation of blood-brain barrier permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150105">
        <property type="term" value="P:protein localization to cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06727">
        <property type="entry name" value="PDZ1_ZO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06728">
        <property type="entry name" value="PDZ2_ZO1-like_ds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06729">
        <property type="entry name" value="PDZ3_ZO1-like_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000009">
        <property type="entry name" value="Putative tight junction protein ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000013">
        <property type="entry name" value="Putative tight junction protein ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000110">
        <property type="entry name" value="tight junction protein ZO-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005417">
        <property type="entry name" value="ZO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005419">
        <property type="entry name" value="ZO-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865:SF26">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN ZO-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01597">
        <property type="entry name" value="ZONOCCLUDNS"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01599">
        <property type="entry name" value="ZONOCCLUDNS2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="83"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="371"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="581"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="12">
        <location>
            <begin position="785"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="180"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="476"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="997"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1163"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="200"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="481"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1058"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1084"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1181"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1191"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1223"/>
            <end position="1233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1247" mass="140529" checksum="5F2EAF747FE83BA5" modified="2024-11-27" version="1">MKYKKFITIMQAAIGIAPLNKRELLPPGRKLWRPSGDHSGTDVFKFYLTKEAQYLCLRRLSAPNYITIIKNPVMEETVWEQYTVTLQRDPKMGFGIAVSGGRDNPNEETGEISIIVSDVLQGGPADGLLFENDRVIQVNTSLMDSVPHSFAVQTLRKCGKVAKIMVKRPRRVPVNLLKRAPSPDDRVFCNPDYSDDYDYDQDRRSVYSRPDHDVGSERGYPDSEYDYDRGSRGRTLERDVSPDRQYSRDGSRGRLLDREHSPDRRYRSDRALDRDRSPDRRYRSEHTLDRDYSPDRNYRSDRGRSPDRRYRSDYTLDRMHSPDERYRGREQRRYEPHQEEPPKRSSSRDRLDHSPSLAPSREPEPLDKPVSVLLLKNRPNEEYGLRLGSQLFIKEMTSTGLASKAGNLQEGDIILKINGTVTENLSLSDAGKLIEKSRGKLQLVVQRDRRQILVRMPPMADSDSELDDISEIESYRSYSPQDDRRGHHSDLSSHSSNERLREKPRDEPPNRLAKMGAKPTPFKVAPDDLPPPPPEEPEVVEPPAAPVVSAAPKISVPPKPKAVPKVPLRPSPEDLEIYGPNTVMMRFQKGDSVGLRLAGGNDVGIFIAGVQEDSPAEVEGLRTGDQIVKVNNMDFRGMVREDAVLYLLEIPKGEDVTILAQSKTDVYADILASGRGDNFFIRTHFDHEKDAPQSLAFGRGEIFKVVDTLYDGKLGSWLAIRTDKDNQLLEKGIIPSKSRAEQMANVQNAQKGAGNDRGDFWRLRGQRAGKKKDLRKSREDLTANPVTTRFPAYERVVLREAGFRRPVVIFGPIADVANEKLANDMPEEFVIAKTEPKDAGSEKSSGVVRLNTIRQIIEQDVHALLDVTPKAVDTLNYTQWYPIVVFLNPDSKQGVKTMRNRLIPGSNRSSRKLYEQAVKLRKTCSHLFTATVDLTSAHDAWYGSLKEAIHEQQNRAVWVCEGKLDGSEEDVDLHDDRMSYLSAMSADYLSMDSRLTSDYDDTADEGGAYTDNELDELTDEPQVPGIGRSSEPVLPDEKPAPVPRMKRAGSKEVLRDSSPPPSFVPEPPKVRSQTRGSDSRSFDSHSSSTVSSDAPISSKPLPPPVALKPSFSARPVPPQGPISEADDPANRSFMGKVKAFEQMDHLARAQRMLELQEAEHARLEISQKHPDIYAVPMKPKPNQNRPQPIGSSSNSESHSSSSKPSYSEGRGPYRSAADDDEEEYRRQLADQTRRGYYNPQKYNDTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L5R8</accession>
    <name>A0AAR2L5R8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spindle assembly abnormal protein 6 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SASS6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017558029.1">
        <property type="nucleotide sequence ID" value="XM_017702540.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L5R8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074557.1">
        <property type="protein sequence ID" value="ENSPNAP00000069721.1"/>
        <property type="gene ID" value="ENSPNAG00000010108.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430207"/>
    <dbReference type="CTD" id="163786"/>
    <dbReference type="GeneTree" id="ENSGT00390000006932"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10142">
        <property type="entry name" value="HD_SAS6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.20">
        <property type="entry name" value="Spindle assembly abnormal protein 6, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032396">
        <property type="entry name" value="SAS-6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038558">
        <property type="entry name" value="SAS-6_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041513">
        <property type="entry name" value="SAS6_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281:SF4">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16531">
        <property type="entry name" value="SAS-6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18594">
        <property type="entry name" value="Sas6_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle assembly abnormal protein 6 N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="SAS-6 coiled-coil" evidence="10">
        <location>
            <begin position="145"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="573"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="622"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="156"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="415"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="650" mass="73632" checksum="3F6D61C509131D03" modified="2024-11-27" version="1">MDLLFNKTLQVQVKCKDCDDRRTFIRVSIELQLTSNPVHRKDLVVRLTDDADLYFLYNLVISEEDFQSLKVQQGLLIDFTSFPQKFIDLLDQCIAEQDKDHPRFLLQLSSTSSALDHSPASLNVVETNAFKHLTHLSLKLLPGSDVEVKKYLAACLATVKQEKEQLQQRLRTTEEDLSRQLSYAQQTLSEKSRELDKLRSEWTSQTTSLSSRHTQDLTAEREKALEIQTKLQQQNDQLRRELEAAHQRSTQQLQVRLSELETSNRELTEKKYKSDSTIRDLKAKVLGLEEESQRAKQQVISLRRENGTLDSECHDKERLINQLQTRVAVLEQEIKDKDQLVLRTKEVLEATQQQKNSVEETSESKQLQIGKLEATVKSLSEELIKANGIIKKLQGDLKALVGKIKVKNTVTVSQEKILQETTERLQREQRELQETQQKLRLKEEEVSKLKEQLEATVQKLDESREVLKTNENVITWLNKQLNENQLSRKQEAVGMFETPQAAAGLRLGTHNMLDSKSFPSLGQPYPVTSTLNSKHSFPLSSLNTGPRSVLTLHNQSAGTKVQFNPISGKPNMPPVEVGTGGLAAQQQSSSKENGEPLGLDSKYFERRDDSIPLRGLVPSMHLNKDVTKPSANMGGVKPALQSAPSAYFPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LC01</accession>
    <name>A0AAR2LC01_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tumor necrosis factor receptor type 1-associated DEATH domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TRADD</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017581097.1">
        <property type="nucleotide sequence ID" value="XM_017725608.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017581098.1">
        <property type="nucleotide sequence ID" value="XM_017725609.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017581099.1">
        <property type="nucleotide sequence ID" value="XM_017725610.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LC01"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086959.1">
        <property type="protein sequence ID" value="ENSPNAP00000074133.1"/>
        <property type="gene ID" value="ENSPNAG00000033684.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108444432"/>
    <dbReference type="CTD" id="8717"/>
    <dbReference type="GeneTree" id="ENSGT00390000002016"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002947">
        <property type="term" value="C:tumor necrosis factor receptor superfamily complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08780">
        <property type="entry name" value="Death_TRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.680">
        <property type="entry name" value="TRADD, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035712">
        <property type="entry name" value="TRADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009095">
        <property type="entry name" value="TRADD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036729">
        <property type="entry name" value="TRADD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14913">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR TYPE 1-ASSOCIATED DEATH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14913:SF0">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR TYPE 1-ASSOCIATED DEATH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09034">
        <property type="entry name" value="TRADD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55044">
        <property type="entry name" value="TRADD, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="8">
        <location>
            <begin position="210"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="293" mass="33233" checksum="CA36778F2B9F73F5" modified="2024-11-27" version="1">MGSTEYTKMAVNSTNDRAWSGCAVLFLRCCNPGSDLLTFYKDKHGKFSMFKVIKLTLTDVAGGLDGYEILKLHDADPFLGVELKFMDALPCKRFLDSYACGSLLQFLSQHASRLLTLPDEVAMEAVLKAGTHILDHDLKNTELCLQHIHQSQPVRLRDDEVTQLEQQLQNSYAPPAQTPQEVPKNCFLFQKRVFDDRPLTSADQQRFAAHVGRDWKRVGRALQKNCRALKGTAIDNLAYEYEREGLYEQAYQLLGRFIQSEGRSARLGRLISALEESKLTNMAEIMLDIQPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LHH0</accession>
    <name>A0AAR2LHH0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine-rich repeat protein SHOC-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein soc-2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">protein Sur-8 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SHOC2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SHOC2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LHH0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051473.1">
        <property type="protein sequence ID" value="ENSPNAP00000074102.1"/>
        <property type="gene ID" value="ENSPNAG00000017556.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156270"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000115">
        <property type="entry name" value="leucine-rich repeat protein SHOC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000327">
        <property type="entry name" value="leucine-rich repeat protein SHOC-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000093">
        <property type="entry name" value="Putative leucine-rich repeat protein shoc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050715">
        <property type="entry name" value="LRR-SigEffector_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752:SF26">
        <property type="entry name" value="LEUCINE-RICH REPEAT PROTEIN SHOC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="13">
        <location>
            <begin position="207"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="13">
        <location>
            <begin position="452"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="591" mass="65712" checksum="BCBBA05E82DB251D" modified="2024-11-27" version="1">MSSALGKDKDCKEKEAKAPSGKEREKEPKTPGSLGKEGKESKTKGKDAKDGKKDASGAPPAVAFSVDNTIKRPNPAQGIRKKSSNAEVIKELGKCRDENSTRLDLSKRSIHLLPSSIKELTQVTELYLYSNKLQSLPAEVGCLSGLVTLALSENSLTSLPDSLDNLKKLRMLDLRHNKLREIPAVVYRVTSLTTLYLRFNRITAVEKDIRNLAKLTMLSIRENKIKQLPAEIGELCNLITLDVAHNQLEHLPKEIGNCTQITNLDLQHNELLDLPETIGNLSSINRLGLRYNRLSAIPRSLAKCRELEELNLENNNISVLPEGLLSSLVNLTSLTLARNCFQSYPVGGPSQFSTIYSLNMEHNRINKIPFGIFSRAKVLSKLNMKWSGPPWTLTEQDNQLTSLPLDFGTWTSMVELNLATNQLTKIPEDICGLASLEVLILSNNLLKKLPHGIGNLRKLRELDLEENKLESLPNEIAYLKDLQKLVLTNNQLTTLPRGIGHLTNLTHLGLGENLLQHLPEEIGTLENLEELYLNDNPNLHSLPFELALCSKLSIMSIENCPLSHLPPQIVAGGPSFIIQFLKMQGPYRAMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LKB8</accession>
    <name>A0AAR2LKB8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Retinoblastoma-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">107 kDa retinoblastoma-associated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">pRb1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">RBL1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. May act as a tumor suppressor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with AATF. Interacts with KDM5A. Interacts with KMT5B and KMT5C. Interacts with USP4. Interacts with RBBP9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573762.1">
        <property type="nucleotide sequence ID" value="XM_017718273.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LKB8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046007.1">
        <property type="protein sequence ID" value="ENSPNAP00000076990.1"/>
        <property type="gene ID" value="ENSPNAG00000012573.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439720"/>
    <dbReference type="CTD" id="5933"/>
    <dbReference type="GeneTree" id="ENSGT00950000183202"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000035">
        <property type="entry name" value="RB transcriptional corepressor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.140:FF:000001">
        <property type="entry name" value="Retinoblastoma-like 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000082">
        <property type="entry name" value="retinoblastoma-like protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015030">
        <property type="entry name" value="RB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF20">
        <property type="entry name" value="RETINOBLASTOMA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01369">
        <property type="entry name" value="Rb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="17">
        <location>
            <begin position="72"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="18">
        <location>
            <begin position="387"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="16">
        <location>
            <begin position="788"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein C-terminal" evidence="19">
        <location>
            <begin position="934"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="632"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="878"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1011"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="893"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1025"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1052" mass="119121" checksum="2D13C80B085FAD0A" modified="2024-11-27" version="1">MRGDETDSESGKSDDGGSIRRSLDALCQELNMDEETASEALQNFTAVWNTYTLEGEVVHWLACSLYAACRKGSTPTVGRGLVEGNCVSLTRILRSSKLSLIQFFSKMKKWADMSNLTQDFRNRISHLERNFEVSTVIFRKFEPIFLDMFQNPQGEPPRLPRGRKHRRLPCHISDVFKFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLVFGNALLCSNKKDLINPHFRGLPKDSGSSEEAPCVIEKLCELHDGLVVEAKGIKEHYFKPYIRRLFEKRILKGDEETLTELLDTPNFQDNNKAINREYEEYVLTVGDFDERVFLGADADEEIGTPRKAAIEPPSGQLSARMQVENNLQQHFEKTRSLAPSTPLTGRRYLKEKELLITPVSLATQSVSRLQSMVSGLRNAPSDALLQIFKACSRDPTETILSRVKTFGETFKQGYTKQTDDLPGAHMDFAENRLKLAEILYYKILESVMTQEMKRLHGKDMTGLLEQEVFHCSLLACCLEVVLFAYSSQRTFPWILETFQLRPFYFYKVIEVFIRSEEGLSRDMVKHLNTIEEQVLESRAWTKDSVLWEALHNANNKVPTVEEVNFPSSFDTGSNSSSGGPTHLPLVALSPIIHPRLREVRTGLGSSARKDVPPSPISLHDRFSSPAAGSAKRRLFGDDPQPQSPVKRISVTPIKIIPSTSAEGSQHANTTTVLSMSTTNGQQLNIPLPVVKNEMGGITVIQVQASDLNPLTAQVLLASPTRAPAPLACPDLQMQPVNRPRRTGSLALFFRKVYHLASVRLRDLCLRLDISAELRGKIWTCFEHSLVHCPDLMKDRHLDQLLLCAVYIISKISKEEHMFQDIMKCYRSQPQASSHVYRSVLLRRRVREQQADENMEVDPPADQSNERTAARPEFDSESEEERGDLIQFYNSVYVLKMQNFALKYALPASDSRTEAPPLSPFPSVRAQRLSPRRISQRHSIYLSPHKNGSCLTPNSAYTYKFSGSPSKELSDINRMIRQGGVSKKRAFTMDGDETESPTKRLTQENDDVLLKRLQDVVSERANH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLY8</accession>
    <name>A0AAR2LLY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">KPNA5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLY8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054195.1">
        <property type="protein sequence ID" value="ENSPNAP00000077560.1"/>
        <property type="gene ID" value="ENSPNAG00000012820.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244950"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000001">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000013">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="166"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="559" mass="62551" checksum="CBBE5B3D089EC1DD" modified="2024-11-27" version="1">MYHEMASPGKENYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKKRNVCVPSGDESMLECPIQDPDVSSTEGVITQDMVQMIFSEDPDQQLIATQKFRKLLSKEPNPPIDEVILTPGVVSRFVEFLKRSDNCTLQFEAAWALTNIASGTFLHTKVVIETGAVPIFIELLNSEYEDVQEQAVWALGNIAGDNAECRDFVLNCGILPSLQQLLAKSNRLTTTRNAVWALSNLCRGKNPPPDFAKVSPCLSVLSRLLFSSDPDVLADACWALSYLSDGPNDKIQAVIDSGVCRRLVELLMHSDYKVVSPALRAVGNIVTGDDIQTQIILNCSALPCLLHLLSSPKESIKKEACWTVSNITAGNRAQIQAVIDANIFPVLIEILQKAEFRTRKEAAWAITNATSGGTPEQMRFSHIAVVLHSKAPVTIVCIIYTHRYLVSLNCIKPMCDLLTVMDSKIVQVALNGLENILRLGEQESKQNGTGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGVEEEDASIAPQVDQNQGQYIFQQPEGPMEGFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LPP9</accession>
    <name>A0AAR2LPP9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LPP9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049615.1">
        <property type="protein sequence ID" value="ENSPNAP00000076714.1"/>
        <property type="gene ID" value="ENSPNAG00000022704.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156713"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000001">
        <property type="entry name" value="Fatty acid-binding protein, adipocyte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="3">
        <location>
            <begin position="28"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="16732" checksum="9358970990FDC820" modified="2024-11-27" version="1">MHWTCLQDVHRQECILCWLNCAVVIFTGVGFATRQVGNVTKPTIVISHEGDKVVIKTLSTFKNTEISFKLGEEFDETTADDRNVKSTVTLDGDNLVHVQKWDGKETKFVREIKDGKMVMVSSHSQILLYYCYSFTSVNVFVNGLIMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LUH5</accession>
    <name>A0AAR2LUH5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mineralocorticoid receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NR3C2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LUH5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055853.1">
        <property type="protein sequence ID" value="ENSPNAP00000078307.1"/>
        <property type="gene ID" value="ENSPNAG00000018585.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159333"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990794">
        <property type="term" value="C:basolateral part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031963">
        <property type="term" value="F:nuclear cortisol receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990239">
        <property type="term" value="F:steroid hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051414">
        <property type="term" value="P:response to cortisol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="NR_DBD_GR_PR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Androgen receptor variant"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NHR-like_dom_sf"/>
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        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
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        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
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        <property type="entry name" value="Znf_hrmn_rcpt"/>
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        <property type="entry name" value="Znf_NHR/GATA"/>
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    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
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        <property type="entry name" value="STRDHORMONER"/>
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        <property type="entry name" value="ZnF_C4"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0754">Steroid-binding</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="604"/>
            <end position="679"/>
        </location>
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    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="721"/>
            <end position="955"/>
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="247"/>
            <end position="356"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="255"/>
            <end position="269"/>
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        <location>
            <begin position="283"/>
            <end position="304"/>
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    </feature>
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        <location>
            <begin position="305"/>
            <end position="349"/>
        </location>
    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="975" mass="105920" checksum="55F4E765BF40BF7D" modified="2024-11-27" version="1">MDLLKMTKRYQSFYEGTDTENRWAQVPRSMEFCCTAEEGSLMNSDVLMDIVNGSNTSNAQVPTCKDTFEKKTEAPILRLNPNQPLSLPAFNNSLHNRKPDADSKELSKTVAESMGLYMNAAREAEFAFGQQGPAAGCQGSPGKMYPLCGQGIEDNRSGAVGSPNLKAPPTTFPTMSQLSNGNLGECVVGTAPAPSALATALSSPIENSSNISSPTGNNIVTSTTSPTYFGASCPSIGSPASLGPVGSGLAPGHRLNSSTCSPVESSTVGSPPLASPLNVMKSPISSPQSMGSVRTPPSCSTNIRSSVSSPTGSGSNQRPSMSSPPTTGSMAMSSPRNPSRSFSVSSPPSGLGLVQNDVHSPEVREPDFKSFEFPKVENIDGEIFNVGLDSMGMIKYIKNEPDTDYRSMCLGSGKSNMSHSPFISQIKIEPNRDMTCAGARYGEPQHPMGLFPGSETLRDNIDEYNLSGILGPPISSLNGNYEPGVFPNNDLPKGIKQETNDGSYYQENNNVPASAIVGVNSGGHSFHYQIGAQGTMSFSRHDMRDQTNPLLNLISPVTALMETWKTRPGLSQGPLSTRGDGYPGPGCLSDNMASASLRHSSSTAKVCLVCGDEASGCHYGVVTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRVRKCLQAGMNLGARKSKKLGKLKSVSEDSVQGSKDTVPFLSSEKELNSMALVPHSPAITPYLSPSVCSVLELIEPEVVFAGYDSTQPDTTDHLLTSLNKLAGKQMIRVVKWAKVLPGFRTLPIEDQITLIQYSWMCLSSFSLSWRSYKHTNAQMLYFAPDLVFNEERMQQSAMYDLCLSMKQVSQEFVRLQLTYEEFLAMKVLLLLSTVPKDGLKNQAAFEEMRVNYIKELRRSVGKATNNSGQTWQRFFQLTKLLDAMHDLVGNLLDFCFYTFRESQALKVEFPEMLVEIISDQIPKVESGMTHIIYFHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LUV3</accession>
    <name>A0AAR2LUV3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LUV3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041661.1">
        <property type="protein sequence ID" value="ENSPNAP00000057501.1"/>
        <property type="gene ID" value="ENSPNAG00000031118.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000044009.1">
        <property type="protein sequence ID" value="ENSPNAP00000071340.1"/>
        <property type="gene ID" value="ENSPNAG00000034157.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000044187.1">
        <property type="protein sequence ID" value="ENSPNAP00000040923.1"/>
        <property type="gene ID" value="ENSPNAG00000034919.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000047000.1">
        <property type="protein sequence ID" value="ENSPNAP00000041618.1"/>
        <property type="gene ID" value="ENSPNAG00000037705.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000048499.1">
        <property type="protein sequence ID" value="ENSPNAP00000078149.1"/>
        <property type="gene ID" value="ENSPNAG00000031160.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000057361.1">
        <property type="protein sequence ID" value="ENSPNAP00000055579.1"/>
        <property type="gene ID" value="ENSPNAG00000037848.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000060370.1">
        <property type="protein sequence ID" value="ENSPNAP00000039668.1"/>
        <property type="gene ID" value="ENSPNAG00000031247.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000061651.1">
        <property type="protein sequence ID" value="ENSPNAP00000046444.1"/>
        <property type="gene ID" value="ENSPNAG00000034255.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000063951.1">
        <property type="protein sequence ID" value="ENSPNAP00000056663.1"/>
        <property type="gene ID" value="ENSPNAG00000037446.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000064253.1">
        <property type="protein sequence ID" value="ENSPNAP00000049706.1"/>
        <property type="gene ID" value="ENSPNAG00000030900.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000065644.1">
        <property type="protein sequence ID" value="ENSPNAP00000065348.1"/>
        <property type="gene ID" value="ENSPNAG00000036467.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000072608.1">
        <property type="protein sequence ID" value="ENSPNAP00000056417.1"/>
        <property type="gene ID" value="ENSPNAG00000035068.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000075531.1">
        <property type="protein sequence ID" value="ENSPNAP00000080355.1"/>
        <property type="gene ID" value="ENSPNAG00000034944.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000086336.1">
        <property type="protein sequence ID" value="ENSPNAP00000077649.1"/>
        <property type="gene ID" value="ENSPNAG00000036172.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000088146.1">
        <property type="protein sequence ID" value="ENSPNAP00000080676.1"/>
        <property type="gene ID" value="ENSPNAG00000036140.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000088382.1">
        <property type="protein sequence ID" value="ENSPNAP00000076262.1"/>
        <property type="gene ID" value="ENSPNAG00000032894.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000277415"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="147"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="292" mass="31671" checksum="15034A2EE1C1712B" modified="2024-11-27" version="1">MATAGFFSCSECGMFSLDPFSTDSHNDSGVCSKCKLVNCLVEKVDKLELRIRGLIRDRQQGSLLAAPGTPGRASTPSTPALEPPQQGEWVTSRRHTSPPVHHAPPVHVSNRFAPLSEAPAEEPVKGTLVIGDSIVRHVKLATPLGAPAARVICLPGARAPDISGNLRLLANRRYSRVVIHVGANDIRLRQSEVTKANIKEVVKQAQTLSEEVICSGPIPMRRGDEAYSRLSSLNRWMSKWCTDNHVGFIDNWLMFEGKPGLLGRDGVHPTRDGAALLSCSIAHSLLVNPMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LW31</accession>
    <name>A0AAR2LW31_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LW31"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063511.1">
        <property type="protein sequence ID" value="ENSPNAP00000080798.1"/>
        <property type="gene ID" value="ENSPNAG00000034536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163299"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000013">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF15">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="65"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="169"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="267" mass="29700" checksum="764CEBF3683D7B63" modified="2024-11-27" version="1">MKRADIVCRQLDCGSAVTTTLRGSGSDEMEWVVSIKCNGCESALRDCTIWDEFQSQVYLICSDSVRLVDGVGRYSGRVEVKSHQSWTTVCEADFDWKDAEVVCRELGCDTPLTLQGALYGEGKLPFGTKKFQCKGTENHLLNCNTSDREENTCTRGKAVELTCSEPDDVRLVDRSSRCAGTVEIFYSGKWRKVIEDGWSLREAAVVCRQLNCGSAAAVGAALREAEREEIAWVIQTDCTGSESALRECKIYYFEDIKFFADVFCSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LXA6</accession>
    <name>A0AAR2LXA6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cystatin fetuin-B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017573335.1">
        <property type="nucleotide sequence ID" value="XM_017717846.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LXA6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077165.1">
        <property type="protein sequence ID" value="ENSPNAP00000078956.1"/>
        <property type="gene ID" value="ENSPNAG00000036370.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108439429"/>
    <dbReference type="GeneTree" id="ENSGT00950000182930"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025764">
        <property type="entry name" value="Cystatin_Fetuin_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050735">
        <property type="entry name" value="Kininogen_Fetuin_HRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001363">
        <property type="entry name" value="Prot_inh_fetuin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814">
        <property type="entry name" value="FETUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814:SF15">
        <property type="entry name" value="SI:CH211-262H13.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51530">
        <property type="entry name" value="CYSTATIN_FETUIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01254">
        <property type="entry name" value="FETUIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01255">
        <property type="entry name" value="FETUIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043366847" description="Cystatin fetuin-B-type domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin fetuin-B-type" evidence="9">
        <location>
            <begin position="15"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="258"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="302"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="311"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="387" mass="42332" checksum="D5EA8B6AF3293636" modified="2024-11-27" version="1">MHSLTWTFLIVHCLGAMATHLSPVPCNDKSVEKLARLAITYINEDRQTGYKFALNRIFNVHVHPQGPAGKVYYLDLDVLETKCHILSPKSWKRCDIRPFMETQISGNCNTTVLHTPDGFSYLYSYDCTLVPDAPEKLQRTCPDCPLLLAIDSDKAVSTAKTTLQRYNYQSTLPVKLTLGDITRASHQISPVAASFVEYTVQECTVTPVEEVLCVPVDAGKGAVGFCVGVVFGADPVQADVKVSCEIFQPQRIGISAVVPQQEEKSTTQQDTPTPPVYVPDAHVPTFQDTSQGGLVDTVQQLRPHPSHLPMSSESSESSETGSSSSEELPGSVRVARPPLDFRYAALRQKRQAPAVIKPLHSPTFLAGFPSTPSPFRSCPGAARYTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M0E8</accession>
    <name>A0AAR2M0E8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polyadenylate-binding protein-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PAIP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037387766.1">
        <property type="nucleotide sequence ID" value="XM_037531869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M0E8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046104.1">
        <property type="protein sequence ID" value="ENSPNAP00000080071.1"/>
        <property type="gene ID" value="ENSPNAG00000021307.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108416001"/>
    <dbReference type="GeneTree" id="ENSGT00940000153432"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="polyadenylate-binding protein-interacting protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051367">
        <property type="entry name" value="mRNA_TranslReg/HistoneTransl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254">
        <property type="entry name" value="EIF4G DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254:SF15">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MIF4G" evidence="6">
        <location>
            <begin position="136"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074029"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="450" mass="49962" checksum="86A08767130B3410" modified="2024-11-27" version="1">MAESFDRAPGAGRTRGKASGPAAGELYSGPPAEQDAGASVFNQCEPLRQPRTSPPTPESSAGSGELQTDRSSTRPSNSAHSNSSTAALKPLSAESLVQNSKLSANAPEFFPTSYPPYQDVVYEDGSDGYYAGASLADFVQEFLSHLNSSPGSFEFTIGQITATLNDWVTTEETLQELVELIFTQSTSMQNFAYTGARLCNHLSHHLALSPASGNFRQLLLKRCRVEFEQRDQAVLGGDETQRRFHSYVLFLGELYLNLEVRSAKGPPSRAEILLAALKDLMDTLFSRPTDANLICAVKLLKLTGSVLEDSWKQSGKSHMDEIIQRIENIVLDAQCSRDVKQMLLRLVELRSSNWGRVHTAAQFSEATPDNDPNYYMNEPTFYTADGTPFTAADPDYSEAYQELLEREDYFPDLYEENGNDSLDGEEDEMDPEIEEAFEKFCLESERKRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNV2</accession>
    <name>A0A3B4BNV2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNV2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014342.2">
        <property type="protein sequence ID" value="ENSPNAP00000000146.2"/>
        <property type="gene ID" value="ENSPNAG00000007305.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230358"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin heavy variable 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
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        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043915725" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000146.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="148" mass="16193" checksum="140BA74BB8C628E6" modified="2024-11-27" version="2">MFSASLLLLLLAAVSSTGVYSVELTQSGPTIVRPGQSMSLTCKVSGYLLTDGRYCTGWIRQPAGKALEWIGVICANGYTSHSNELNSRFEVTRDTSSNTVFLRGQNLQTGDTAVYYCAREQHTVRHISRNPVQKLLLGTDHHCKGLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPH1</accession>
    <name>A0A3B4BPH1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">LSM14A mRNA processing body assembly factor a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LSM14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPH1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014000.2">
        <property type="protein sequence ID" value="ENSPNAP00000000354.2"/>
        <property type="gene ID" value="ENSPNAG00000000205.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154415"/>
    <dbReference type="OrthoDB" id="21539at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01736">
        <property type="entry name" value="LSm14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000006">
        <property type="entry name" value="Protein LSM14 homolog A isoform b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025761">
        <property type="entry name" value="FFD_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025768">
        <property type="entry name" value="TFG_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586:SF2">
        <property type="entry name" value="PROTEIN LSM14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586">
        <property type="entry name" value="SCD6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12701">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01271">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51513">
        <property type="entry name" value="FFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51536">
        <property type="entry name" value="TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="DFDF" evidence="8">
        <location>
            <begin position="251"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="FFD box profile" evidence="9">
        <location>
            <begin position="325"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="TFG box profile" evidence="10">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="136"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="291"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="364"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="FFD box" evidence="5">
        <location>
            <begin position="325"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TFG box" evidence="6">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="236"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="397"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000354.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="429" mass="46856" checksum="97BD41F38BB2393A" modified="2024-11-27" version="2">MSGGTPYIGSKISLISKAEIRYEGILYTIDTENSTVALAKVRSFGTEDRPTDRPILPRDETYEYIIFRGSDIKDLTVCEPPKPTLPQDPAIVQSSMGSSSAPTSFQCGNSYGLFKRAPVSPYSQFTNSPLVSPPFGAVGARSSPPLESLQKSPPLEPAAPPAPSMSAAAPASVGRRSPIAVRLTTSGNQKPLDILEQRRGEDQPRLWSGHKHATGNKASVVYSSFQSATAGPSAPRRGRGGGHRGRGRFPVRRDGPMKFEKDFDFESANAQFNKEEIDKEFQSKLKLKDEKTEKAVNGEDKGDSGVDTQNSEGNAEEEDPLGPNCYYDKSKSFFDNISCDDTRERRQTWAEERRMNAETFGIPLRHSRNRGGFRGRGAMGFRNNRGRGSRGSFGSSHTGGSGFRGGFRGGQGGREFSDFEYRKDNKVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BS37</accession>
    <name>A0A3B4BS37_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NHLH2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017561994.1">
        <property type="nucleotide sequence ID" value="XM_017706505.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BS37"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001326"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012351.2">
        <property type="protein sequence ID" value="ENSPNAP00000001326.1"/>
        <property type="gene ID" value="ENSPNAG00000008099.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432572"/>
    <dbReference type="CTD" id="4808"/>
    <dbReference type="GeneTree" id="ENSGT00940000164480"/>
    <dbReference type="OMA" id="MECAPLP"/>
    <dbReference type="OrthoDB" id="10067827at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000027">
        <property type="entry name" value="Nescient helix-loop-helix 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040238">
        <property type="entry name" value="TAL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864:SF10">
        <property type="entry name" value="HELIX-LOOP-HELIX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864">
        <property type="entry name" value="T-CELL ACUTE LYMPHOCYTIC LEUKEMIA/STEM CELL LEUKEMIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="67"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="125" mass="14472" checksum="5A5D05EEB7D514E7" modified="2018-12-05" version="1">MMLSPDHSDSDLLWPQSDPETLLNVIKVERAADKPPEVGADNKSRCRSLAPPLTREEKRRRRRATTKYRLAHAMRERIRVEAFNVAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSQ1</accession>
    <name>A0A3B4BSQ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ankyrin repeat domain 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ANKRD22</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BSQ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002727"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009940.2">
        <property type="protein sequence ID" value="ENSPNAP00000002727.2"/>
        <property type="gene ID" value="ENSPNAG00000001285.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009005"/>
    <dbReference type="OrthoDB" id="2157354at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042802">
        <property type="entry name" value="ANR22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47276">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47276:SF1">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002727.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21732" checksum="1B3B650ED6E9E15D" modified="2024-11-27" version="2">MGILYSEPICQAAYNDDIHQVHQLVKEDDKNLNVQDEIYGDTPIIAACRSGSLKTVKYLLHQNADVSVRNKKERTCLHYVAKRRFSFLDYLMIIILMPILLIGYLIMIEKQKAHVSLMKSVLSSKVEVDAVDYKGNTALHYACQSKSHQIIPLLLEKNADISIKNKNDESPMDVAERLNFRKVIAMLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTF0</accession>
    <name>A0A3B4BTF0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA recombination and repair protein Rad51-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BTF0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001790"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011537.2">
        <property type="protein sequence ID" value="ENSPNAP00000001790.2"/>
        <property type="gene ID" value="ENSPNAG00000008369.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167287"/>
    <dbReference type="OMA" id="EMTITPW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28653">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28653:SF1">
        <property type="entry name" value="ATPASE SWSAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001790.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25107" checksum="E6DAF1178CF38768" modified="2024-11-27" version="2">MVVRENPSRSAVSEILRPARLAPTTTPRSKFVYPKTLDDLLEDVASLHELVAEAAALPSLVIVDSLERYVCGPAAQNRPQQETQSAAAHVVALLHDTAAFLTQNLENEAQCRVIVSIQPEWKGQGGCDQLVLDPILFVLERYLQVRCTLEKLRIGEEQNKWLLCLSGPGLQVDGNMSTLARSGGQPYPQRPGSNWGLGVLLKNTSVMYRWRWGSNRQPSGHRAGSLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTU3</accession>
    <name>A0A3B4BTU3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">pancreatic elastase</fullName>
            <ecNumber evidence="12">3.4.21.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CELA1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.36" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BTU3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009345.2">
        <property type="protein sequence ID" value="ENSPNAP00000003072.2"/>
        <property type="gene ID" value="ENSPNAG00000003113.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234528"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000017">
        <property type="entry name" value="Chymotrypsin-like elastase family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050850">
        <property type="entry name" value="Peptidase_S1_Elastase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257:SF0">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043534337" description="pancreatic elastase" evidence="13">
        <location>
            <begin position="17"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="21"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003072.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="273" mass="29384" checksum="34B3D79F852DD35B" modified="2024-11-27" version="2">MLRILYFTTFAALGECYETHVLAGYTSICSLPQVSLRVQSSTCYNHVCGGVLIKTNWVLTAHQLQNICFKFRTINLFVLCSIAVSLVVLGEHDLTASERKEQFFGVSAIYIHPSWNSDLTAGNDVALIRLTSNAILNSYVQLATLPPAGQILSGNTACYVTGWGFTQNGGFLSAQLRQASLPSVDYATCSSSTWWGAAVKTTMICAGGAGTAAGCNGDSGGPLNCVVGGRYVVHGLTSFVSTAGCNTIRKPTVFTRRIIISFYIFSIIRIINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2C6</accession>
    <name>A0A3B4C2C6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thymocyte expressed, positive selection associated 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2C6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004749"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006505.2">
        <property type="protein sequence ID" value="ENSPNAP00000004749.2"/>
        <property type="gene ID" value="ENSPNAG00000011137.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160763"/>
    <dbReference type="OMA" id="RDGPRAK"/>
    <dbReference type="OrthoDB" id="6088188at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029325">
        <property type="entry name" value="ITPR-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043444">
        <property type="entry name" value="TESPA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469:SF1">
        <property type="entry name" value="PROTEIN TESPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469">
        <property type="entry name" value="SPERM SPECIFIC ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14722">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01257">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ITPR-interacting" evidence="2">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004749.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="510" mass="56691" checksum="12E2A017E82DA3E0" modified="2024-11-27" version="2">MCAEDAEETLYHLGFGCEEPQVTARIPARFFTFPSKLRGINFRLFLESQLRRIREEDPSLSLASRFRQVEVLTAMANAFYSLYSHVSRTPLQKLAPPEFSFSSPTVERSIGQRFFGNIRSEPRSPVERLKDTVSKMCLYTGSRGSDSASPNSSPRKRNSLPDVVGMVLGTAKNEGGSDHPRRAWLQDVADNMAQRAGDKNTLQSAEVKTAEKVTNPHKERIQRWSQSGQRDGPRAKASLFKPVCVSASLESTQTEDSSSGTVVNEGSTCSTLLSATLNKPIDNKPTVTIITYDVISPQVVEYVHQAPYRCPQMDNRATVASPPDVRIHRESSCTRTLEPKDKPAVTDARPRVCFSTKDENLVQGPSEVPQDDPVPLCPRTSRRSPCRIMVTGWDGDTTTLKREPSEDFVSPLCSGSTPHLTSLGNQRYLSPIKPSTSQMKQANSFELEEVQSAGEEENLSAEQCGAHTLSVTAATQQKCESYQLPSFSRPHTNNFVKYRFKTSSVFYMQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3N9</accession>
    <name>A0A3B4C3N9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Semaphorin-6B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Functions as a cell surface repellent for mossy fibers of developing neurons in the hippocampus where it plays a role in axon guidance. May function through the PLXNA4 receptor expressed by mossy cell axons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017562936.1">
        <property type="nucleotide sequence ID" value="XM_017707447.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037387048.1">
        <property type="nucleotide sequence ID" value="XM_037531151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3N9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005663"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004931.2">
        <property type="protein sequence ID" value="ENSPNAP00000005650.1"/>
        <property type="gene ID" value="ENSPNAG00000011815.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433102"/>
    <dbReference type="CTD" id="562398"/>
    <dbReference type="GeneTree" id="ENSGT00940000159170"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
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        <property type="term" value="P:central nervous system development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:neural crest cell migration"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of cell migration"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Sema_6B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Semaphorin 6B isoform 3 variant"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="semaphorin-6A isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Plexin_repeat"/>
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        <property type="entry name" value="Semap_dom"/>
    </dbReference>
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        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Semaphorin"/>
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    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF130">
        <property type="entry name" value="SEMA DOMAIN, TRANSMEMBRANE DOMAIN (TM), AND CYTOPLASMIC DOMAIN, (SEMAPHORIN) 6BA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PSI"/>
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        <property type="entry name" value="Sema"/>
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        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
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    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
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    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043938199" description="Semaphorin-6B" evidence="18">
        <location>
            <begin position="21"/>
            <end position="873"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="617"/>
            <end position="721"/>
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            <begin position="775"/>
            <end position="796"/>
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            <begin position="620"/>
            <end position="631"/>
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    </feature>
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        <location>
            <begin position="640"/>
            <end position="655"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="708"/>
            <end position="721"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022782"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022902"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="873" mass="96962" checksum="872045798A77447E" modified="2024-11-27" version="1">MATGAPFLPMLLGILRLATCSFPEEPGPLISAPAEVARRYPVFLGRAHRSYMRQEPLYIQSVLKVNRTLYIGARDDLYRVELDHVSGDEMFYSKKRTWESNKNDIRICRMKGKHEEECRNYMKVLLSQHGGLFVCGTNAFNPLCANYTRDTLEMVGEPISGMARCPYDPKHANVALFAEGSLFTGTVTDFLAIDAVIYRSLGDGPALRTIKHDSKWFREPYFVSAVEWGPHIYFFFREMAMEFNYLEKVMVSRVARVCKGDLGGSQRVLEKQWTSFLKARLNCSIPGDSHFYFNLLQSTSPIIRMHGRDIILGVFSTPPNSIPGSAVCAFDMQQLARVFEGRFKEQKSPESIWTPVPDELVPKPRPGGCAGQGSRFSSSTAFPDEVLNFIKTHPLMDEAVPLLGHRPWIVKTMVRYQLNTMVVDTEAGPHRNRTVVFLGSTRGTVLKFLIIPGRDSPYSNTNIFLEELEGYNPERCGDDSIQARQPLSLTLDRPSHTLLLAFHSCVVRLPVARCQLHSRCMKNCISSRDPYCGWTRGSTCSLLRPGTRLPFVQDVEYGNITHLGDCDGLLHESFMDEPESLVTLNLLVVAAVSAFSTGAALSGLAVCWIMGQKHRHRSRSNTSSSSSGGNQRKSEKERSMLGPSGSGSVMSVSRHSVAERSRPQGETLFVMPNGWVKAGDLDPGLLPTPEQTPLQQKRPPPGLLRLSDSGSGWDQSQTFLNSVGTQCPPPPPSAIFLSSKLLQGAGDRRHDDTVDRQRYVSLSRYREQAGRPGTLLRKSAGDYDYPVTPQDSPDRRRVVSAPSTQMEYGGETLRWTHEGYIFSHAAPPSGHHYGPAGPHLTRSVLHGSRGLVDLAEFSHLLGKGGVERAPSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4A0</accession>
    <name>A0A3B4C4A0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMC5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4A0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005409"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005347.2">
        <property type="protein sequence ID" value="ENSPNAP00000005409.2"/>
        <property type="gene ID" value="ENSPNAG00000001858.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244894"/>
    <dbReference type="OMA" id="SLPHAYF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF5">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="411"/>
        </location>
    </feature>
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            <end position="439"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="562"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="7">
        <location>
            <begin position="352"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005409.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="601" mass="68382" checksum="2527E23F6F39AC11" modified="2024-11-27" version="2">KLPNRARMQAIRSLPMSFSERRNIRLFMVIFLTVLNLLCQHFVRHTLPLKCGSWASVRQRLVIWQDTLKEIGGRFGTSVLSYFQFLKWLLMFNIFSLLINFCFITIPQIVRSPNITHPISFRGLELLTGAGYFNQTVLFYGGYNGEVIKTQPSYNMQLAYLFTIAAYLVLCGVSLIYSMARSFQRNFVLVAGPAYGGAWRLLCSWDFSVINEKAIQHNKDNLSIQLKESLSERLQKKAVVSVSAKLQQISLQLLAWLLSLGLAVGSCTGIYFLELNLNDISDSGNLEEEASTLLVPVVVSLINLVIPLIYALVNKMEHYTNPRTNVYVIILRNVLLKMSILGILCYYWLSDCWESFVGQSVYRLVVVDFIFCLLGSFFTEFLPNIIGTKCFRSLGVPEFDVATNVLNLIYAQTLAWIGIYFSPLLPVIQVVKLFIIFYLKKVSLSMNCQPPKRTGRAAQMQTVYIALLFFPSFVGALSMVAYTVWSLKPSEQCGPFQNLGKPFQVVLDWMKGVQNIPGSEWAWWTFEHVLRNELFFYLITLIVLVAFLAVENFLIALSRKSYIVIIIVIVLSPSPRLMFLCKKLEETSLPEENEAAAPKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CB85</accession>
    <name>A0A3B4CB85_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MYL4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Myosin is a hexamer of 2 heavy chains and 4 light chains.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572855.1">
        <property type="nucleotide sequence ID" value="XM_017717366.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CB85"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007694"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001400.2">
        <property type="protein sequence ID" value="ENSPNAP00000007694.1"/>
        <property type="gene ID" value="ENSPNAG00000013450.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439132"/>
    <dbReference type="CTD" id="4635"/>
    <dbReference type="GeneTree" id="ENSGT01030000234570"/>
    <dbReference type="OMA" id="YDRTPKC"/>
    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000019">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000056">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF1">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="2" id="KW-0518">Myosin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="187" mass="21010" checksum="13A5628D6C99478E" modified="2018-12-05" version="1">MAPKKVEPKKEAAKSAPPPEPEKPKEPEFDPKGIAIEFTPDQIEEFKEAFMLFDRTPNGEMKISYAQCGDVMRALGQNPTNAEVMRVLGKPKPEELNTKLMDFETFLPMFQHISKSKDRGTFEDFVEGLRVFDKEGNGTVMGAELRHVLQTLGEKMTEKEVEQVIAGQEDGNGCINYEAFVKHIMSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CBZ3</accession>
    <name>A0A3B4CBZ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557093.1">
        <property type="nucleotide sequence ID" value="XM_017701604.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CBZ3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009467"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035331.2">
        <property type="protein sequence ID" value="ENSPNAP00000009467.1"/>
        <property type="gene ID" value="ENSPNAG00000014959.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429677"/>
    <dbReference type="CTD" id="445070"/>
    <dbReference type="GeneTree" id="ENSGT00940000164791"/>
    <dbReference type="OMA" id="CTIPLWR"/>
    <dbReference type="OrthoDB" id="8830244at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="216" mass="22869" checksum="489B4E053FA70445" modified="2018-12-05" version="1">MATTGMQLLGLILSIIGWVAGFLVCALPMWRVTAFIGNNIVTAQITWEGLWMNCIVQSTGEIQCKVYDSLLALPSDMQAARGLTVLSLLLCTLALTLGILGVKCTKCVGHQSPKARLARVSGIIFAVAGFLYLIPVCWTAHSIIRDFYDPYVAAPHKRELGPALYLGWGGSALLLIGGSLLYAGSSPPGIPSSPTFSSGESSPRRAGGPTQVKGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CG72</accession>
    <name>A0A3B4CG72_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ZU5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MACC1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017537993.1">
        <property type="nucleotide sequence ID" value="XM_017682504.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537994.1">
        <property type="nucleotide sequence ID" value="XM_017682505.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CG72"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010325"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016955.2">
        <property type="protein sequence ID" value="ENSPNAP00000010325.1"/>
        <property type="gene ID" value="ENSPNAG00000015846.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411111"/>
    <dbReference type="CTD" id="346389"/>
    <dbReference type="GeneTree" id="ENSGT00390000013151"/>
    <dbReference type="OMA" id="PPMLNHD"/>
    <dbReference type="OrthoDB" id="10000126at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056183">
        <property type="entry name" value="DEATH_SH3BP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056181">
        <property type="entry name" value="SH3BP4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056182">
        <property type="entry name" value="UPA_SH3BP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15603:SF1">
        <property type="entry name" value="METASTASIS-ASSOCIATED IN COLON CANCER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15603">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24094">
        <property type="entry name" value="DEATH_SH3BP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23637">
        <property type="entry name" value="SH3BP4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23640">
        <property type="entry name" value="UPA_SH3BP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZU5" evidence="2">
        <location>
            <begin position="215"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="848" mass="96279" checksum="A20606129570BA91" modified="2018-12-05" version="1">MANVRSKSIRRAGSLLRSRSEGTLIDLDDNITINNNNLHGTYIAQHMGKNLEWPVLQPEVQRSAQTTNPFWSELSRSNPFLDDIVCRNTTDSGVSILKEDPLALFGNGNEDSESTSSDENNFGHLLEPRRNNFNRSGRWRSASDILDDLEKKESKKEKSLNTQAPFLKPDFEWLKNDREAYKMAWLSHRQLTRSCLDLDLMKQSPGWAQTQATDMQTVCKIDHNGGSVQLPDCDITAHVPQGHIPPGEIQEMALKASLDPPRGINSNYTATVSPLLEVSLSNLNTTESITLDIKMAAEVKNDPLSQVMTTFVGLVAQRKEGPYEKVKDCYVYKDMLQMKLQELKPHMYIIAAAEATVIQPPATSVWDYLQRHITVAVYGPKHIHPSFKVVQVISWHNNVPPRLPFSDFQKGNRNLPPVVLQLWGKHRFNPCGLKDLHITTSVVDSRFEVKHTDQNKEVKKEQLKMAKVIHLPLELSKTDGGEMGPFRLVVQVKDSNSLPLTDFHVTSPEAAPLRSDKHGHKHLDRHREAPIPEESIPEFPKFQDVVVNVQWYGVTLKSVLRQPRVDYLLEYFKGDTIALLSRETVKSVGQSRVKEWYIGFLRGSIGLVHYKNIKIIPRDQVIDFSGVKVTTQSLLDNMTLPFRKLTYMYSAIQTLVTDHVTCWRTFAEALGYSNLSVDTFSRRHAETVAEKVACVLEKLKEDCHTDKTKRKFQHELIVGLFKMDAQGLIAHLIQNTVILSTAVELGVRWRELAERIGKLTNAQIAGYEVPHRGKNGEVSAQSMWKPAYDFLYSWSVQYGDGYRDMIQDLHLALDKMKSPVTRHWRQLTGALITVNCMEVLRASAFHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGJ2</accession>
    <name>A0A3B4CGJ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="4">2.3.2.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutaminyl cyclase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Glutaminyl-tRNA cyclotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017573609.1">
        <property type="nucleotide sequence ID" value="XM_017718120.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017573610.1">
        <property type="nucleotide sequence ID" value="XM_017718121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGJ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011107"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017959.2">
        <property type="protein sequence ID" value="ENSPNAP00000011107.1"/>
        <property type="gene ID" value="ENSPNAG00000016729.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439624"/>
    <dbReference type="CTD" id="564655"/>
    <dbReference type="GeneTree" id="ENSGT00390000003107"/>
    <dbReference type="OMA" id="CAHWDSR"/>
    <dbReference type="OrthoDB" id="3907302at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03880">
        <property type="entry name" value="M28_QC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000029">
        <property type="entry name" value="Glutaminyl-peptide cyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037457">
        <property type="entry name" value="M28_QC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF3">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="15">
        <location>
            <begin position="154"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="393" mass="44175" checksum="61070F67742D1758" modified="2018-12-05" version="1">MSRRSRAAAASTLRCAAAGTCDQRPVCRARVLLLCLCILLAFALTLGLILSENETSSLEDMPIRVPDLNKDKLTHRPSKPTAAQVKRLVSEVNWSRLWYAQLQPFLIERHPGSRGSRTVRRHISSLLDSLSAGWSVEIDSFNSKTPRGPINFSNILAVLDPNAPRRLLLACHYDTKIMPLASRDPKQVFIGATDSAVPCAMILELVTALDIHLKMLKQQMAQVTLQLVFFDGGEAFEKWTPSDSLYGSRHLAEHMSRIPHPPGSEHTTLLHAVDLFVLLDVIGAPSPMFVNHFENTARWFEQLVSAEKRLHKLGLLSFHPSEQIYFRKDVIMDPVEDDHMPFLQRGVPVLHMIATPFPSYWHTMEDTADKVHSHTVENLTKVLVVFLAEYLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CHD9</accession>
    <name>A0A3B4CHD9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MOGAT2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CHD9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009894"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016381.2">
        <property type="protein sequence ID" value="ENSPNAP00000009894.1"/>
        <property type="gene ID" value="ENSPNAG00000015373.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234582"/>
    <dbReference type="OMA" id="NDPKHAG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003846">
        <property type="term" value="F:2-acylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006651">
        <property type="term" value="P:diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001878">
        <property type="term" value="P:response to yeast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF74">
        <property type="entry name" value="2-ACYLGLYCEROL O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="29488" checksum="8928610539607759" modified="2018-12-05" version="1">MSFRLLRAKVKAETLKQPEPLVKTADLDPRQNYVFGFHPHGILVAGAFTNFCTQATGFSKLFPGLESHLLMLPLWFRAPFFRDYIMCAGLIPSDKDSASYLLRKEGGGNAVVIAVGGALEALDSHPGTHSVLLASKKGFIKLAIEHGAHLVPVYSFGENEVFDQVANPRGTWLRWIQDRLQSIMGVSLPLFHARGIFQYSFGLMPYRKPITTVVGKPIKVEKKEKPSAEDLDALHQLYMDELTQLFEEHKGKYGIPEDAHLDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIS8</accession>
    <name>A0A3B4CIS8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIS8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011323"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036087.2">
        <property type="protein sequence ID" value="ENSPNAP00000011323.2"/>
        <property type="gene ID" value="ENSPNAG00000033056.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230338"/>
    <dbReference type="OMA" id="HTARCVE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052309">
        <property type="entry name" value="C-type_Lectin_Domain_Fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46490:SF6">
        <property type="entry name" value="ASIALOGLYCOPROTEIN RECEPTOR 1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46490">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 12 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="6">
        <location>
            <begin position="117"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="37"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011323.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="239" mass="28060" checksum="F61B51033E75C7C0" modified="2024-11-27" version="2">MRRYYRLTAVCLGLLCVILLAAITVLWVKFNNLTIDRDQLQTSYTNLTVERDQLETSYTNLTTERDQLQTSYTSLTTERDQLQTNNDNMKNEMGQLQKEKETLQKKLSELEQKQRCFRDSFYYISTEKKSWTESRKNCTERGADLVIINSREEQEFITKAFGSSEAWIGLMDTEKEGVWKWVDNSTLTTEFWWTGEPNDYEGKENCAVTDLWFVSNKNVQTWADYPCNHPAVGICEKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIX6</accession>
    <name>A0A3B4CIX6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ZFYVE21</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017550924.1">
        <property type="nucleotide sequence ID" value="XM_017695435.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIX6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010786"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035854.2">
        <property type="protein sequence ID" value="ENSPNAP00000010786.1"/>
        <property type="gene ID" value="ENSPNAG00000016417.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426142"/>
    <dbReference type="CTD" id="79038"/>
    <dbReference type="GeneTree" id="ENSGT00940000159639"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15727">
        <property type="entry name" value="FYVE_ZF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.160">
        <property type="entry name" value="Zinc finger FYVE domain-containing protein 21, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052113">
        <property type="entry name" value="FYVE-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032031">
        <property type="entry name" value="ZFYVE21_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038632">
        <property type="entry name" value="ZFYVE21_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490:SF8">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16696">
        <property type="entry name" value="ZFYVE21_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="244" mass="27397" checksum="8059019FCD955FA2" modified="2018-12-05" version="1">MSAVPDGKKLVRSPSGLRMVPENGAFNSPFSLDEPQWVPDKECPRCMQCDTKFDFITRKHHCRRCGRCFCDKCCSKKVALPRMCFVDPVRQCGECSLISQKEVEFYDKQLKVLTAGGTFLVTVGSSEKSETMACRLSNNHRYLFLDGESHFEVELSRISSMQVLTEGSTPGEKDIHTYTTLLDSDYISEGGLSRATGMLLQYKPPGSQDAQQLRMEAADDKRIASAWLAAMHKAAKLLYESRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CN66</accession>
    <name>A0A3B4CN66_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PCNA</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Component of the replisome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574386.1">
        <property type="nucleotide sequence ID" value="XM_017718897.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CN66"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012004"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019153.2">
        <property type="protein sequence ID" value="ENSPNAP00000012004.1"/>
        <property type="gene ID" value="ENSPNAG00000017726.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440157"/>
    <dbReference type="CTD" id="5111"/>
    <dbReference type="GeneTree" id="ENSGT00390000004965"/>
    <dbReference type="OMA" id="EMKLINM"/>
    <dbReference type="OrthoDB" id="534348at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014823">
        <property type="term" value="P:response to activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000008">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000001">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.150.10">
        <property type="entry name" value="DNA Polymerase III, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00293">
        <property type="entry name" value="PCNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="12">
        <location>
            <begin position="127"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="260" mass="28641" checksum="4E64AFE395BE7A4D" modified="2018-12-05" version="1">MFEARLVQGSILKKVLEALKDLITEACWDVSSSGISLQSMDSSHVSLVQLTLRSDGFDSYRCDRNLAMGVNLSSMSKILKCAGNEDIITLRAEDNADSLALVFETLNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSQIGDAVMISCAKDGVKFSASGELGTGNIKLSQTSNVDKEDEAVTIEMNEPVQLIFALNYLNFFTKATPLSKTVTLSMSADIPLVVEYKIADMGHIKYYLAPKIDEESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNM3</accession>
    <name>A0A3B4CNM3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CSK</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 22">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNM3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012179"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036418.2">
        <property type="protein sequence ID" value="ENSPNAP00000012179.1"/>
        <property type="gene ID" value="ENSPNAG00000017944.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157431"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05082">
        <property type="entry name" value="PTKc_Csk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09937">
        <property type="entry name" value="SH2_csk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11769">
        <property type="entry name" value="SH3_CSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000272">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000023">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035027">
        <property type="entry name" value="Csk-like_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="9"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="82"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="195"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="450" mass="50930" checksum="65B89861C60B680D" modified="2018-12-05" version="1">MSGLQTAWPTGTECVAKYNFQGTMEQDLPFSKGDVLTIIGVTKDPNWYKAKNTLGREGTIPANYVQKREGVKSGGKLSLMPWFHGKITRDQAERLLYPPETGLFLVRESTNYPGDYTLCVSCEGKVEHYRIIYHNGKLSIDEEEYFENLMQLVEHYTKDADGLCTRLIKPKLMEGTVAAQDEFSRSGWALNMKELKLIQTIGKGEFGDVILGDYRGTKVAVKCIKHDATAQAFIAEASVMTQLRHNNLVQLLGVIVEDRGSLFIVTEFMAKGSLVDYLRSRGRTVLGGECLLKFSLDVCEAMEYLEVNNFVHRDLAARNVLVSEDNIAKVSDFGLTKEASSTQDTAKLPVKWTSPEALREKRFSTKSDVWSYGVLLWEIYSFGRVPYPRIPLKEVVPRVEKGYKMDAPDGCPAVVYDLMKQCWTLDPAVRPSFRMLREKLQHIRAKELYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPC4</accession>
    <name>A0A3B4CPC4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HMX3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcription factor involved in specification of neuronal cell types and which is required for inner ear and hypothalamus development. Binds to the 5'-CAAGTG-3' core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HMX homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPC4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013205"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036832.2">
        <property type="protein sequence ID" value="ENSPNAP00000013205.2"/>
        <property type="gene ID" value="ENSPNAG00000003571.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158286"/>
    <dbReference type="OMA" id="YLERASW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000053">
        <property type="entry name" value="H6 family homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051300">
        <property type="entry name" value="HMX_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="142"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="144"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="93"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013205.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="276" mass="31171" checksum="5ADC6A22ABDCBA65" modified="2024-11-27" version="2">PPTTTTRPSAKDSPFSIRNLLTCDSKASANPTNPAKADLDTALSISRLGDFAFPRFERFGLAGQCLERSPAWWWCYPYTLNSPGHLHTAAVSERPIRPPSPSPTDQSSPEPRTKEEEEEEEEEEEEEEAAQEEVQEEEKRPSRKKKTRTVFSRSQVFQLESAFDAKRYLSSSERACLASSLHLTETQVKIWFQNRRNKWKRQLAAELEAANAHHHHHHHHQQQHRIVRVPVLYRESAESARGGATGGGVGGGVGQPLLFPYYPHPIMPSVPLLRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWH2</accession>
    <name>A0A3B4CWH2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWH2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023951.2">
        <property type="protein sequence ID" value="ENSPNAP00000015688.2"/>
        <property type="gene ID" value="ENSPNAG00000020723.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183127"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019882">
        <property type="term" value="P:antigen processing and presentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000353">
        <property type="entry name" value="MHC_II_b_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF99">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DRB1 BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00969">
        <property type="entry name" value="MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00921">
        <property type="entry name" value="MHC_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="128"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015688.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="264" mass="29831" checksum="5D91AA2E84000E45" modified="2024-11-27" version="2">MNWSSVSQTLSVVGCRDKMLLSLQLLLLLTLTIRTDGYYFYTRYECSSSSSDLTDAEYIETYHFNKDPFLRFNSTVGEYVGYTELGVKNAERWNKGSDVQQARGELERYCKNNLPMLFSNILEKTVKPRIKLVAEQQASGGHPAMLMCSAYDFYPPVIDVYWLRNGEKVTSDVTATEEMADGDWYYQIHSHLEYTPTSGEKISCVVEHASSKEPIIIEWDGFLPESERNKIAIGASGLVLGIILSAAGFLYYKKKSSGRNLVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CX28</accession>
    <name>A0A3B4CX28_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CUB domain containing protein 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CX28"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037910.2">
        <property type="protein sequence ID" value="ENSPNAP00000015888.2"/>
        <property type="gene ID" value="ENSPNAG00000003704.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010209"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038811">
        <property type="entry name" value="CDCP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056266">
        <property type="entry name" value="CDCP1_CUB_3rd_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056268">
        <property type="entry name" value="CUB_CDCP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056269">
        <property type="entry name" value="CUB_CDCP1_2nd_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14477">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14477:SF1">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23665">
        <property type="entry name" value="CDCP1_CUB_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23667">
        <property type="entry name" value="CUB_CDCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23668">
        <property type="entry name" value="CUB_CDCP1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="564"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 first CUB" evidence="3">
        <location>
            <begin position="2"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 second and fifth CUB" evidence="4">
        <location>
            <begin position="60"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 third and sixth CUB" evidence="2">
        <location>
            <begin position="163"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 second and fifth CUB" evidence="4">
        <location>
            <begin position="347"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 third and sixth CUB" evidence="2">
        <location>
            <begin position="453"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015888.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="744" mass="83566" checksum="EA7348651F66982B" modified="2024-11-27" version="2">HINRSQTAQSKCEVCIGKLCQHSVTLINNTEVVFNCAHPEDIFTVEIVKQIGRDRCNEYPSLQNFSRTFTWNVSTQAQKAFSLNFTKAGLRQIRPTESCQDKHVYTVSAGRTTLGRFCRHGTIKGMKVSNEGSVSLQVPGKGQMDPTTFDISVGDHIPLLPIIQVSLPEEESSQEFLSPNYPDSFPDNDLVTWSFRVPPKYYTTVRIQNYTDPKCQRKDARLEYQLYFKRVVKKLSETQLSEYQGSFNLSLQNCPMQPSSALSLHFSISAIKRGSEDLRKEKELQIHIKKRKPKSACVLKLNSQIQETATIFSSRISYLSFYDCAEEDLLLNINQNIGRVLMVPVLEKCLLGVLQQVTWRLYVPQHGAVELMFPLGSQQQSLAGQTCNSSVLLTVTEHEQGNITVGQFCPQGPIHKVQIRSNITVTASPVDSNDLRQITYALLKGSFMETINEHYIFTVLPKKDTSVLLATPAWPVGMRPYSTVSWIVSFPSQLEAQVVFTNVSQVSCPNQHKGIKVQKQGSRDETYSWRIGEKLKDVVVEESFYLNMSNCRPSKGSFSVVNKILSITLSVLSVLLVLVVAVLAVVCIINRKKKKQAAPEVSVYNPNGHAFLPGLHGIPKTTEDEDVHIYHCIDDTMVYGHLLKDGTEMNHFEPAVDSYQAFTGPTERQPLTEPEVGVYRLFTGLEKGYVSGLVLADASVLLNPCPSKGASTTLHLSYDTNGKVLKILLWRRNWETKKKMVVCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CXX9</accession>
    <name>A0A3B4CXX9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protocadherin gamma-C3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PCDHAC2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CXX9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038021.2">
        <property type="protein sequence ID" value="ENSPNAP00000016188.2"/>
        <property type="gene ID" value="ENSPNAG00000022295.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163777"/>
    <dbReference type="OMA" id="CPGSTHY"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000185">
        <property type="entry name" value="Protocadherin 2 alpha c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF304">
        <property type="entry name" value="PROTOCADHERIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043837094" description="Protocadherin gamma-C3" evidence="14">
        <location>
            <begin position="24"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="675"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="21"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="128"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="233"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="341"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="446"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="571"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016188.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="951" mass="104646" checksum="B9970F55C874A8D1" modified="2024-11-27" version="2">MELWVLRASCPALMLSLLGCALAQLRYSVPEELEHGAFVGNVAEDLGLDPNELFLRKFQLVSGARRRYLEVNLENGVLFVNERIDREQLCEQSPSCFLHLQAVLENPLELHRVEVEVLDVNDNAPSFPRAGFSLEISESAAPGSRFPLESAQDADVGENALRSYLLSVNAHFALDVRAGKFAELVLQRPLDREQQGAHHMVITAVDGGSPEHSGSAHLNVTVVDANDNAPAFERESYRAKVVENAPRGTVVIRLNATDPDEGPNGEVTYSFSGHAPAKVRELFSMDPRTGEVRVKGVVDYEKATVHEIYVQAKDKGPNAIAVHCKVLVTVVDVNDNHPEVILTSTSAPVQEDALPGTVIAVISVMDQDAGENGHVDCEIPHHVPFQLHSSFKNYYTLVTSDFLDREKVPDYNITLTARDMGSPPLFTRKTILVQVADVNDNAPVFRQPSYTIYLAENNHPGASIFTVSAQDADGGQNSYLTYSILDSEIQGALASTYVSINSDNGNIYAMRSFDHEQLKSFQLLVQAKDSGFPPLVTNATLNVFVLDQNDNAPVIVSPLPENGTEAASEVVPRSADAGHLVAKVTAMDADEGQNSRLLYQMLQATDFSLVSIALYTGEVRMIRRLRDGDPATHRLLVLVKDSGEPSLSATASIILSVVDSEPEVGDLFQHPRDRALYLIVSLGAVSFTFLVAIIVLAAVKGYRHRLSKYDSSCCGFSSKASTAEDIKSNLNVHISPTPNGAADPMEVNGSTQLGQNYCYKMCLTPESSKSDFMFLKPYSPAASTLHVNNSKVMENNISSWSPEIHQLNINYADASQREIKQANKDWTLLKNQRNYSHKSSATVVKTLQRRVILDHSDTCTCPGSTHYWTWGSHMWDYKTSSEAGGLTDRCWTPRFDYQHNVYIPGTPGLLKAAGHGELDIYNSFSTFGKKKRITPSHDLHEDTVITNELYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CY87</accession>
    <name>A0A3B4CY87_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dermatan-sulfate isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037386748.1">
        <property type="nucleotide sequence ID" value="XM_037530851.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037386749.1">
        <property type="nucleotide sequence ID" value="XM_037530852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CY87"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015756"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024024.2">
        <property type="protein sequence ID" value="ENSPNAP00000015756.2"/>
        <property type="gene ID" value="ENSPNAG00000021804.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432251"/>
    <dbReference type="CTD" id="568099"/>
    <dbReference type="GeneTree" id="ENSGT00390000006522"/>
    <dbReference type="OMA" id="KYMDRMS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047757">
        <property type="term" value="F:chondroitin-glucuronate 5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.100">
        <property type="entry name" value="Chondroitin AC/alginate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008929">
        <property type="entry name" value="Chondroitin_lyas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052447">
        <property type="entry name" value="Dermatan-Sulfate_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15532">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15532:SF2">
        <property type="entry name" value="DERMATAN-SULFATE EPIMERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48230">
        <property type="entry name" value="Chondroitin AC/alginate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043646737" description="Sulfotransferase domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="762"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="793"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015756.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1207" mass="137366" checksum="99896E27092C9E39" modified="2024-11-27" version="2">MALECMLKSGVLLCLMTLGFSFTVLHNTSSKYTPVVESMEQGKLQEEKYMPSHVHPNLYFDLTEVKQLTKKAGSTHGHIFRIIQNAVNTMLENPTLYLPPMSHEDFGKKWNEIYGNNLPPLALYCLLKPEDSAASQFLIKYMDRMSEYPDWMVSTAPHDEVPMAHSLTGFATAYDFIYTYLDTQRRVRYLKKIRAVTEELCELSKHRGWGRQFLQNHQTTNILAILTGAIVTGVHKDPESMIWKQIAVNYMEKTMFLLNHIVDGSLDEGVAYGSYTAKSITQYVFLALRHFDIDNRRNNWLRAHFMFYYATVLPGFQRTVGIADSNYNWFYGPESQLVFLDAFVLRNGSGNWLAQQIRKHRPKDGPMVASVAQGWATLHTELIWYDAELSPKPPLHFGKANMHVFSNWGVVTYGAGLPYAQDNTFVSFKSGKLGGRAVYDIVHAKPYSWVEGWSNFNPGHEHPDQNSFTFAPNGQVFVAEALYGPKYSYLNNVLVFAPSPTSHCNKPWEGQLGECSKWLRWGEKEEGDSSGEVISASSHEDTLFVSGEAVSAYSSAMKLKSVYRALVLLNSQTLMVLDHVEKQEQSPVTSFSAFFHNLDIDLRYVPHISLDRYSGTLMDVWDAHYKMFWLDSQGASPVPRIQEVEQVAEFKKRWTQFINVTFSMDTSVSRVVYLMHGPFVKVSDCRFIDNGKNGVRVSVTLNGTETTVSIATNYKDIGARHSYLGFAGFAKAESKHRIIRFGQGIQTLPSQEKRESTFDIRFVFSIITVWILCFAFVFSSFKRKFNISFSKIIRLILISVLFFWVLELLVMSHSCGEVLCGVTWQDLSVSDEVVTRTTIPDHTPDPLPFIVMTSLPGSGAEILQHLFDNSSDFVYLRIPSPYLHVPETNFVLDSLVDACEWSRADAQSRKFLAVQGWFHSMMHSIRMHLQNIQLYKSNLEAGSKVSSAQGSSKKSRKLLMEPRATMRRRPADQDVEYIKGLRQHLVTYPNACPVLNFRSGSWSLKLPFIHEVIGRSLRSVQVVRDPRAWIYLMLYNSNPSLYSRINIKKHLLNIVNQSSMEAGQGCTRFDPAFQPLRRISGQPDASPMLLLAHLWLAYTSASLRASMDLPPHTYLRVKFEDVVLFPEETAGMIHEFLGIPIAPAAINQLTFAASTNLYHLAYEGDISPAKINMWRENMASDEIKLIEDICWPVMETLGYQRHAVMHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D039</accession>
    <name>A0A3B4D039_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Krueppel-like factor 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Interacts with FBXO38.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576821.1">
        <property type="nucleotide sequence ID" value="XM_017721332.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D039"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016411"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024915.2">
        <property type="protein sequence ID" value="ENSPNAP00000016411.1"/>
        <property type="gene ID" value="ENSPNAG00000022575.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441679"/>
    <dbReference type="GeneTree" id="ENSGT00940000155235"/>
    <dbReference type="OMA" id="QQTIWEM"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21585">
        <property type="entry name" value="KLF7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000021">
        <property type="entry name" value="Basic krueppel-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001395">
        <property type="entry name" value="Krueppel-like factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF77">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="200"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="230"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="77"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="283" mass="31378" checksum="E4FEDB9139AEF517" modified="2018-12-05" version="1">MDVLANYSIFQELQLVHDTGYFSAMPSLEENWQQTCLELERYLQTEPKRLSDLFEDELDCLLSPGFTKADSEEDMMDPLLSTLSNPPSPPPPLLSPPREPLTSPSAPLPSLDLGTTVNAAQLNAVTSLTPPSSPELGRHLVKAAHTLCASPDGTLTLKLVARKVASARTTSPEHGGGHSDGEQVGSAGGGDLAENKKRVHRCQFNGCRKVYTKSSHLKAHQRTHTGEKPYKCSWEGCEWRFARSDELTRHYRKHTGAKPFKCAHCDRCFSRSDHLALHMKRHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0E7</accession>
    <name>A0A3B4D0E7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Serine/threonine-protein kinase receptor</fullName>
            <ecNumber evidence="17">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017552685.1">
        <property type="nucleotide sequence ID" value="XM_017697196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0E7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017792"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026804.2">
        <property type="protein sequence ID" value="ENSPNAP00000017792.1"/>
        <property type="gene ID" value="ENSPNAG00000033016.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108427208"/>
    <dbReference type="GeneTree" id="ENSGT00940000156210"/>
    <dbReference type="OMA" id="ILALWMY"/>
    <dbReference type="OrthoDB" id="547665at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048179">
        <property type="term" value="C:activin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048185">
        <property type="term" value="F:activin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017002">
        <property type="term" value="F:activin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23632">
        <property type="entry name" value="TFP_LU_ECD_ACVR2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000099">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000094">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF70">
        <property type="entry name" value="ACTIVIN RECEPTOR TYPE-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00653">
        <property type="entry name" value="ACTIVIN2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017232816" description="Serine/threonine-protein kinase receptor" evidence="18">
        <location>
            <begin position="23"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="188"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="512" mass="57766" checksum="AFB58ABC69FE0BFE" modified="2018-12-05" version="1">MFARWLTLALIRGSLLAGPGHAEVGTRECVYYNDNWRAERTNQSGYERCEGEKDKRQHCYASWLNSSGTIHLVKKGCWLDDFNCYDRHECVATEESPQVFFCCCEGNYCNERFTHLPEAPAVKIQPPPAGPSLLGVLVYSLLPLVVLCLALVLACWTYHQRKPPYGHVDIIQDAGPPPPSPLVGLKPLQLLELKARGRFGCVWKAQLLSEYVAVKIFPIQDKQSWQNERDIYLTEGLRHENLLHYISAEKRGTNLQMELWLITEFHERGSLTDYLKGNVVNWAELCHIAESMARGLAYLHEDLSYRLEGPKPAIAHRDFKSKNVLLRTDLTAVIADFGLAVRFEPGKPPGDTHGQVGTRRYMAPEVLEGAINFHRDAFLRIDMYALGLVLWELMSRCTAADGPVDEYLLPFEEEIGQHPSLEDLQDVVVHKKMRPILKDCWLKHPGLAQMCETVEECWDHDAEARLSAGCVEERIATIAKATNTTNTSTSECLVSMVTSDSDSELPPKESST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D486</accession>
    <name>A0A3B4D486_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine rich repeat containing 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LRRC7</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="42514.ENSPNAP00000019157"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028803.2">
        <property type="protein sequence ID" value="ENSPNAP00000019157.1"/>
        <property type="gene ID" value="ENSPNAG00000005806.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156262"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098968">
        <property type="term" value="P:neurotransmitter receptor transport postsynaptic membrane to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098887">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000118">
        <property type="entry name" value="Leucine rich repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000061">
        <property type="entry name" value="leucine-rich repeat-containing protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF48">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="691"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="864"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1211"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1276"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="691"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="707"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="720"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="746"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1280"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1526" mass="171551" checksum="845C4A018F2B37A8" modified="2018-12-05" version="1">MDNCSTLQLASFRNQQSGATEALEWCWTVQGLEMTTKRKIIGRLVPCRCFRGEEEVISVLDYSHCSLQQVPKEIFSFERTLEELYLDANQIEELPKQLFNCQALRKLSIPDNDLSNLPATIASLVNLKELDISKNGIQEFPDNIKCCKCLSVVEASVNPITKLPDGFTQLLNLTQLFLNDAFLEYLPANFGRLSKLRILELRENHLKTMPKSIHRLSQLERLDLGSNEFTELPEVLEQIHNLKELWLDNNSLQTIPGSIGKLRQLRYLDLSKNRIESLDADISGCESLEDLLLSSNMLQQLPDTIGMLKKLTTLKVDDNQLTSLPNTIGSPRAKLGLSLLEEFDCSCNELESLPPTIGYLHSLRTFAADENFLAELPREIGNCKNVTVMSLRSNKLEFLPDEIGQMTKLRVLNLSDNRLKNLPYTFTKLKDLAALWLSDNQSKALIPLQTEAHPETKQRVLTNYMFPQQPRHDEDYQSDSDSFNPALWEEQRQQRMTVAFDFEDKKEDEDNSAKVKVPLANFVEINLKRYPTPYPEDLKNMVKSVQNMVGKTTHPLSSSGTNSSGTNMELINKDKYEPKWPIAPKEVTDREVKDFSKPPMPEQAVIRNSAIDIPKRKDKEDLNESSEISLHRVVPKPQQPENTSLAVTSSSCFCLIDSMGGSPNDIRISDMRPTLVEPPMYKPKVVLLGKDKKESTDDSDPDKMHCLNNSGSSATYSDYSPSQGSSGSSNPPGNVSSLQSAAKEGPTQTHWTNRLAQSFPKPIESKPLLSQREMPPSSTLQQRGERRPLSDTFDSWNDAPHYDNTGFVAEETPLETPSGSSGNPMLGTKPRSASTAHGRRPLMRQERIMGVPLELEQSQHTFRNVPDTEVPPPPNPWQNWTRTPSPFEDRTAFPSKLELTPANSPNPDRKDFEQEMGELPGTFPSTGAWGFLDSRDSGTAQGQPNIFSHVQGRKEPNKGTTMVISKSSERLSPMMKEVKSKFKKSQSIDEIDIGSYKVYSIPLDSYSSSIENQGSVDRADLPGPLEQSMSRSQSAPMLDDDLDGFSMNKNSQQKPAIPKKVYQFDQSFNPQGAMDVVKPDRRVPPPFPNTPEYVNQPGKTLPKELVSPRGYRGYQPMEQMFSFSQPSVTDDAITPQFTQPQRSRPGFLRRADSLVSSTEMALFRRVAEAQEHQVAEHYSRPQQLKGMLEHQNSLSSMVDTQFHKRNGRYEEEYSSYQEPKKPIMGYPTKSLTQRRPLSARSYSTETYGASQARPVSARPTMAALLEKMPSDYNLSTCTEKSPDADTKLRPVSQKPEDPTSKMPVDWRQQLLRHIEAKRLDRSAVLKHNTVNFGMLYSGGYAAPHASRSMTINFYNNTKHENQTTQWLPLPNTPSQQSNILDNGQEVVSPTTQWAPYSLGRRDVPPENLNIIKKAAIQHPHQQQGMMVTSASATPPHRTARDQQYEGAMNKVSIQQYQSPLPMPITSTSPRPQSARCFIQTKGQKSMDGYQEQFCVRIEKNPGLGFSISGGISGQGNPFKPSDMGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4Z6</accession>
    <name>A0A3B4D4Z6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Dystrophin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">DMD</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">May play a role in anchoring the cytoskeleton to the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 17">Cytoplasm</location>
            <location evidence="1 17">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000019422"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039471.2">
        <property type="protein sequence ID" value="ENSPNAP00000019422.2"/>
        <property type="gene ID" value="ENSPNAG00000026008.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154342"/>
    <dbReference type="OMA" id="SACERYT"/>
    <dbReference type="OrthoDB" id="10057795at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000118">
        <property type="entry name" value="Dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000008">
        <property type="entry name" value="Dystrophin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000001">
        <property type="entry name" value="Dystrophin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000023">
        <property type="entry name" value="dystrophin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000004">
        <property type="entry name" value="dystrophin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000032">
        <property type="entry name" value="utrophin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000056">
        <property type="entry name" value="utrophin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000070">
        <property type="entry name" value="utrophin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000075">
        <property type="entry name" value="utrophin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000044">
        <property type="entry name" value="utrophin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035436">
        <property type="entry name" value="Dystrophin/utrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF25">
        <property type="entry name" value="DYSTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002341">
        <property type="entry name" value="Dystrophin/utrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 17" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 17" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 17" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="22">
        <location>
            <begin position="33"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="22">
        <location>
            <begin position="152"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="21">
        <location>
            <begin position="2973"/>
            <end position="3006"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="23">
        <location>
            <begin position="3226"/>
            <end position="3282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="3430"/>
            <end position="3450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="3496"/>
            <end position="3548"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="1046"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="1213"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="1295"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="1995"/>
            <end position="2042"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="2840"/>
            <end position="2877"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="3393"/>
            <end position="3430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="3496"/>
            <end position="3535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR002341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019422.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="3601" mass="414785" checksum="8E176D1AFBF6DEC8" modified="2024-11-27" version="2">MARAGSPEDQWEVEAFEDEFGEIIKTRSDEREDIQKKTFTKWINSQFAKAGRAPIDDLFIDLCDGRRLLELLEGLAGHELVKERGFTRVHSLNNVNRALQVLQKNNVELVNIGGADIVDGNHKLTLGLIWSIILHWQVKDVMKDVMADLKQTNSEKILLSWVRQSTKNYPQVNVMNFSSCWEDGLAFSALIHSHRPELFDWAVVEQMDKAIDRLDHAFRIAEKHLGIERLLDPEDVATSHPDKKSIIMYVTSLFQVLPHGVSMEAIKEVETLPRTMVTKEEHFHFQTQQCYSQQITVSVAQSEVRSPSPSSKPRYKSYAFTQAAYVKTPEQKRKNPVTQSPEKLEDMWPSLSPLPQGSTQLESYQSALEEVLTWLLSAEDGLQAQPPISSFVEEVKEQFHTHEGYMVELTSHQSSVGRVLKAGSILLAEGHLTEDEESEIREQMNLLNTRWEHLRVASMERQSRLHEVLMDLQHQQLKQLTTWLDVTEARIKRMGAQPLGPEIEDVKHQIEEHKLLQEDLEMEQVRVNSLTHMVVVVDEKSGESATAALEEKLQNLGERWAAICKWTEERWILLQKILMSWHHFTEEQHLFDSWLTQKEQLVLSIQSTTDPNEIAANLRKLTILKADLELKRQNMDKLCSLVQDLLSNIKNKEAAGKLEARLERFAQRWDKLVQSLNLTSTKFTTTVTTSQSELTQKTVETKVTTHQKKIVKHTKDGLATPPPQKKRQIVVDSELRKRFDVDFTEIHSFMTRSEAILQSPEFSVSRKEGSVADLYDKNITIFDTAIDKERPEKFRKLQEATRSAQALVDQLTTDGQNCDDIQQAEQQLRTRWIDFCALLAERLAWLAYQTKVLAFYSLFQELEQAVANSENWLKVQQPPACEQEPLRIQLERCRDEIARLSAHQPQVGQLQKQLQGLREKEETPVIFDADITAFQDHYYQVLADLKAREKQLVLVQSSLPPVRYKDVMAALMAWLQQCEAKLALPSTAVTEYPVMEQRLKDMMAIHAAQQEHQCEVEDLNKMAEQVFQKAPPDMCQKYHIELDNVMARWRRAGEQVEENIQKLQEHMAKLQQFQNDTKTLQKWMADVDVFLNEEWPALGDSEALEKQLEQCTALVNDIHTVQPSLNGINEVGQALKREAETPYAIKIQRMLDELNAQWELICKQAYAKKSALKGGLDMTVSLKKEMQEMQEWITQAEEDYLERDFQYKTPEELHKAVEELKRAQEEVHQKEVKVNLLTDKVTSFISKAPQAAHDALKTELDVLTSNYHRLCSRLDGKYKTLEEVWACWCELLAYLEQENAWMDLLEKRLDETENLQRGAEEIAEALASLDTMIREHPEYNRNQIRELAQTLMDGRVLHELIHRKVEDYNIRWDELMQRALQRQQQLEKSLQWAQENDNTLRLIRDSLNTTDRHLTAYLVDGIDAAQIPQEAQKIQTDLTGHEVTLDDMKKKASEADASDKLLAEIDATYNKLLEVKAKFRLFQKPANFDQRLKECERVLEDVKGQLGVLSICSFEQEVVQSQLEQCMKLYKSLSEVKSEVEMVIKTGRQIVQRQQTEQPKELDGRLTSLKLLYNDLGAQVTDGKQELEKSLKLLRKFRKEVNSLTEWLAAIDGELTLRSSVEGLPSDLEAELTWAKATQEDTERHTPQLRSVRELAEALKALLRGQESLIDDKVSLLNCNWIAVTSRSEQWLNLLLDYQGQMKTLDQNVAQINAWMDGAEAQMDKMESQGCIEHIIKGLQTELEEMRVKLEAIQSLGEDLIKNRGEHCKAQVKPKLEQLSHRFETVAERILNAQASTHEVEEYYRQAQMWLQLLDEEIKLGENVKEDDFLEEPAVDEDALKKLFLRGENLLKRTTEPANSTVREKHNLLHDRYNTLKNLRALRRKKALAFAPQWYQFHKKSDDMLQFLDNIEKMVEELPDPEEPTVKEIGTKINQQRPELKDVQQLGKVLSEAGMVKIVEPRLIPINKRWDQLDNKFAQMHHMSVRMAKEKSSDVSNLVDEVQALLRSIAENEERLNSPVSWSAAKENLHQQENSLKEVKQNLDILETPVVEALDWNKVHLHENLLIHLLNTNWENLKRLYQDRLAYVNKCRKYSDELKDLQGWLTAAEKTLVLYEEDPNLNRSHLKDLQDVLEKQEAAVKALNVMGEDLAMQCRKEDGDHIKQQLASINTRWAKLFSELTEIKRRSAGEKIVLTELHEDIGEFQSWLDKAEGVAAIQVQPGNKEQLCTALEKVEAQVAELPSRKQALHKINIKGSSLHADKVKPLENNIKIINMRWAKVSSDLSEKQRQIEDLLRNLSLYQEQLSKLSNWASTTRSQLEQSSGDINHKIDDDIQSKKSDVETVLATERPPCQPERGQFDSLNADWMAIQVLLKEWRDKCQVSVESLTGSVTGDATTMDKFNKSWSDLNNWLSLLDNMVLTQRVTVGDLADISGMTIKLKSSLQDMEQRCPQLNKQITAAQNLKNKTNNPQTRATITDQIDKLQAHWEDSHTKLLDRVLQLQNMHQDSSDWLEARKRVEPLIKQANEKLEVWKGTSQSADDLKKLAKNIQQWQAQMDVTNELANKLLTHYAVDDTSKIKQMTESMNVAWANIKKRVADKEADLEPKQRQLQHFYIDLEKFINWLTEAETTANVLQDAAYKQRPLDNPATVCHLLEQWQDLKMEIDAHTDMYHSLDENGQRIMASLEGTDGALMLQKRLDNMGQRWHDLRKKVINMRPHLDASMEQWKHFHMSLQELLHWLQLKKEDLDQQKPVGGDVPTVQQQLITHKAFRRELNTMEPSVKGALENVKSFLIEMPSEEAKQRPGQRDIRTEERVQNVERILRKEAEDVTTQWKVLGPAAVEWQRQLELALERLMELQDAEEQLDFKLQQAEMVKDSWDPVGDLLVDNLCKQIDIVKAFQEEIAPIQDNVNQVNKLASTFKTPGVQLSPDNLNRIEDLNMRWRLLQISIEGHLTHLTEAQRDLGPHSQNFLQASVQSPFERSVSPNNVPYYINHQTQTTCWDHPKMAELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLGMSTACESFEQHNLKQNEQFIDIMQMINCLTSIYDRLEQQHSSLVNVPLCVDMCLNWLLNVYDTGRTGKIRTLSFKTGIISLCKAHLEDKYRFLFRQVASATGFCDQRRLGLMLHDSIQIPRQLGEVASFGGSNIEPSVRSCFHFANNKPELEASVFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMQYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMVILICISVPASSPQLSHDDTHSRIEHYASRLAEMENRNGSYVNENISPNESMDDEHLLIQHYCQSLSQGSPLSQPQSPAQILISMEGDEKGELERVLNDLEEENRKLQAEYDRLKKAHEHKGLSPLPSPPQRLPVSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLKRLRQLLEQPQTESKLNGTALSSPSTASQRSDTSLASLRVAASQTTETMGDDELPSPSQDASTGLEEVIEHLNNSFPHSQGNKMESLFHMVNNIGYAMESLVNVLTAQQSRVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6G9</accession>
    <name>A0A3B4D6G9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NF-kappa-B inhibitor delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NFKBIZ</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6G9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019033"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039263.2">
        <property type="protein sequence ID" value="ENSPNAP00000019033.2"/>
        <property type="gene ID" value="ENSPNAG00000025531.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153695"/>
    <dbReference type="OMA" id="MGAMAPL"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000097">
        <property type="entry name" value="NF-kappa-B inhibitor zeta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF7">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="283"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="417"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019033.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="66204" checksum="E62F44A51EA26C3D" modified="2024-11-27" version="2">MHLQKSPKEKSCYTLPTVKKLLEQKRKRETSSAPPACAAASSSAGAVAPVPVSPSAPSDGTSNSYFDMAAMSYDRWDGLLHQHSSALQPCNSFSAVALGYPQSFSQTGVYSHSLPTAEPQASSYSPHQLQDCQDGQIPQNFPGAECPVTAALSGANAVTVQSSCYSWSSGSRPQAPAGPCSLQFRTQVDIAKLEEARAFLQGMDYSRTTWQDDDGDTILHIYTAKGQREYAFAAAEKLQELGRLDSKEHKGKTALLVAVTANQPEIVQDLLSLGADISICDNKGQTALHLAATYGFPQVMQMLLSMDHGLDMEARNFEGLTPLHCAVISHSATMKALAASSSSSTWLPDSGLQSQAENKLFCLQLLLDAGASPVSQEIKSNKTVLHLAVKEGNIQLVHFFLKFQLPDMQAFINMKAHGHTALHMAAGLHGNPHQEELIRLLLSRGADPSIRNLENDQPAHLLQSGQQGEKAIKPLQLIQNVAAQLVFNLPKFSHVTPLLRSLHWLPLAARIRFKTLTLAYKAKNGPAPAYLMAMVKSQTVPRALQASSTARLDPPSFKAHGRQASRMFSALAPRWWNELALAVRTAESLAVFKRRLKTHLFTQHLNEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6Q1</accession>
    <name>A0A3B4D6Q1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">BICRA</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017541169.1">
        <property type="nucleotide sequence ID" value="XM_017685680.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000020042"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030285.2">
        <property type="protein sequence ID" value="ENSPNAP00000020042.1"/>
        <property type="gene ID" value="ENSPNAG00000026784.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413253"/>
    <dbReference type="CTD" id="29998"/>
    <dbReference type="GeneTree" id="ENSGT00940000159112"/>
    <dbReference type="OMA" id="FERVSCQ"/>
    <dbReference type="OrthoDB" id="2556847at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052438">
        <property type="entry name" value="Chromatin_remod/trans_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015671">
        <property type="entry name" value="GSCR1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572:SF1">
        <property type="entry name" value="BRD4-INTERACTING CHROMATIN-REMODELING COMPLEX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572">
        <property type="entry name" value="GLIOMA TUMOR SUPPRESSOR CANDIDATE REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15249">
        <property type="entry name" value="GLTSCR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="678"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="738"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="935"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1328"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1460"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1514"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1583"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1663"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1789"/>
            <end position="1845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="678"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="749"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="852"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="935"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1396"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1601"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1801"/>
            <end position="1824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1835"/>
            <end position="1845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1845" mass="192032" checksum="5744C4F6551D0DD3" modified="2018-12-05" version="1">MDDEDGRCLLDVICDPQALNDFLHGSETQLDTDDLLDGSSDPSSSFFSSAGGHVPEVQPQAQLSTSEPSGLPRVSVDLDFLEDDDILGGSPGGGGGDGGSNGVGSNHEPCDILQQSLAEANITEQSLQEAEAELDLSSFGLTSLTQVVQPLPDAALPGVGIGGATQIFPGQGTTTTPSSATTDMLGSVLAHPGLQIQPQVMNKAISVQPFMQQVGLGNVTLQPISSLQALQNGSQTGHLGIGQIQVVGQPTVMTINPSGQQILTKTMGGYQLQQPGPDAANVGAQAGLGGSVLSSGGGLLIQGGKATLGSPSLNGPAVCVSTTNTNSSGTTMAAASGIVGFSSAPLSTGIGSQTQPQPQSQIMQNVIIQRTPTPIQPKPPQGGTIQPKSFKQQQQQLPTPHALQNDANKALGVQQVPASAAQNVAFLTGKTGSNVVLSTQTTTQGTQFPQALFKQQGAPATGKPLSVHLLNQQGSIVIPSQAVLQGQNHQFLLPQLQTGGQILTQHTGGHIITSSGPGGQIIAANQILTANQNINLSQVLASQGHPGAAHILSGPIQLQSGQMGHPTLFQMPVSIAQTQNQAHPVTGHVQTVIQGMPIQNSVSIEGLSPAVSLQPTLQQQAGGIPTNSSTGAAAMAQCQPGENIAMLGSSTEQAVQPQVHPQTQPQTSILTVQTGPSVSASVSVPSSTAPSSTVTTSTSSVTTVGLGPQAQHSPGKVLFTPPGSSMILSQEPVQMFLQQDQQHQAGKDPPASVGVPASVIVSGGSSGSAPTGPDNLLAETHPGQSPSPSLGPAHMAALVNKVPSAAHQQALKIQSASPSQTVAPHSVTPTLSDSPQPSQASPLMLGQQIQSPHPPPQSRPPSQPQAQPPSQSQTPTRACTPSSLPPLFIIHNQIGGSPQPPQLAAQSAQQTQPQQIQVQIPQVLPQPLTQPVILQQEQPPSSSCSPKPPTAPPTFQFATPTVGTPTGQVAKQQIAVPGLSTEQQHHLQLINAQLQTMSSIAQPSLQQKQLLEKLHQLQQNIILQAKQQAQAQGQVLQPQAASQFSKLPEQSAAQVVTSSAGTAISGSVPPLLQQKSVLVKSSATGSNDTQVLSGASGPAVVSVNQGIAPPNLAQAVQAKTGVISGVGGLTLGKPGIQIQVLSSGLSQMPAPQPPAPVQTQTAALKRPFSMEPSKEARMLEQLRKLQGSVLHPDYSSPFCSFEDTLHRLLPYHLYQGMAPSPQDYRKVDDEFENVSCQLLKRTQAMLDKYRHLLFEESRRLGPSAEMVMIDRMFIQEEKIALNQDRILAKEKPEEFVANSCLLNRTADSAVRPTPVELSSVRTGPSAVAAAHVTATPSPAPPSVTPASTAAPTPSPFPPTKLVIKQGGGGALVSWSTSCSPTPAAVVRPSPEPAAQGSERSFGRTSSSTRSADDDDDALPQRTSKPPIKTYEARQRIGLKLKIKQEAGFSKVVHNTALDPVHSQTQHTPQPQTPEPLSKSRPHVTQTATVIRTPPPTCNPTSSTIFSAVTTHASSTSCGTASSTAPSSSVSHSWSSSSSSSTCTAQMNGTLEHNEMGGVKGNPTSTSTPQLTTCRLPLRKTYRENISPRHRPGVPGGGDILPIPPPAPPGPLTSSPKSERTVIASVKLERQGGSGPTQPHNDPGSQGLMAVEDEFFRGIKNAYHHHHHQQQQQFSDKEDEEDGDSRDCVGRVKGLVSKNRERALGTFQMDQHAPGPPSPGETSCTRDSSLPAKRCKSDSPDMDNASFSSGSPPPDDSLNEHLQCAIDSILNLQQGPGRGPMGRVHATNLSNQHQTHHRQGTSSSSSSTYRHSLSSSSSPSSSSMSQHPQVGGRGQNGSLVSQTHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6Y0</accession>
    <name>A0A3B4D6Y0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">SEH1 like nucleoporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SEH1L</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572415.1">
        <property type="nucleotide sequence ID" value="XM_017716926.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6Y0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028867.2">
        <property type="protein sequence ID" value="ENSPNAP00000019193.2"/>
        <property type="gene ID" value="ENSPNAG00000003951.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438850"/>
    <dbReference type="CTD" id="81929"/>
    <dbReference type="GeneTree" id="ENSGT00940000153393"/>
    <dbReference type="OMA" id="NAPTRRW"/>
    <dbReference type="OrthoDB" id="364224at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000063">
        <property type="entry name" value="SEH1 like nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF3">
        <property type="entry name" value="NUCLEOPORIN SEH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="19" id="KW-0137">Centromere</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="16" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="15" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 20" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="20">
        <location>
            <begin position="8"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="20">
        <location>
            <begin position="274"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="384"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019193.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="45891" checksum="E75BFC24D7DEFA48" modified="2024-11-27" version="2">MFVARSIAADHKDLIHDVSYDFHGRRMATCSSDQSVKVWDKSDNGEWHCTASWKTHSGSVWRVTWAHPEFGQVLASCSFDRTAAVWEEIVGESNDKQRGLSHWIKRTTLVDSRTSVTDVKFAPKHMGLVLATCSADGVVRIYEAPDVMNLSQWSLQHEISCKLSCSCISWNPSSSRAHPPMIAVGSDDNNVTYSGKVQIYEYNENTRKYAKAETLMTVTDPVHDIAFAPNLGRSFHVLAIATKDVRIFKLIPLRRENATSSGPTKFEVQVMAQFDSHNSQVWRVSWNITSTLLASSGDDGCVRLWKANYMDNWKCTGILRGDGSPVNGSSGQGTALSAVGSAGAQSQNSVVGAASAGRYFFPHLDPPRAGSRLALLLPPPSLIEPTCETDHTHAHQPPQIRRRYTPALPTQPAEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7C5</accession>
    <name>A0A3B4D7C5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mammalian ependymin-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">EPDR1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds anionic lipids and gangliosides at acidic pH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ependymin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569703.1">
        <property type="nucleotide sequence ID" value="XM_017714214.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7C5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019338"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039420.2">
        <property type="protein sequence ID" value="ENSPNAP00000019338.1"/>
        <property type="gene ID" value="ENSPNAG00000025870.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437243"/>
    <dbReference type="CTD" id="54749"/>
    <dbReference type="GeneTree" id="ENSGT00940000161910"/>
    <dbReference type="OMA" id="TMKDCYP"/>
    <dbReference type="OrthoDB" id="6084362at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001299">
        <property type="entry name" value="Ependymin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697:SF1">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00811">
        <property type="entry name" value="Ependymin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00317">
        <property type="entry name" value="EPENDYMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00026">
        <property type="entry name" value="EPEND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017328275" description="Mammalian ependymin-related protein 1" evidence="5">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="214" mass="24277" checksum="530B9788690EDFDF" modified="2018-12-05" version="1">MSRFTVLTLALCLSGVAVCFVRPSLSAAAPPQPCAAPLQWEGRTVQYDHNTGRNTRAVVSYDGQNQRLRVLEEKRKHTPCKRYFEYIFLFNSGVLFQIEQVTKTCAKLNLTEEWDPYDIPMNSTYEDQYFIGGPGDMIEVQEWSDRKPARKHETWVGVYTLKDCYPVQETYTKNSSMTTSTRFFDLALGISDPGVFVPPSSCLSAIPERMASDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7T0</accession>
    <name>A0A3B4D7T0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">BCAM</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017557529.1">
        <property type="nucleotide sequence ID" value="XM_017702040.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7T0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029354.2">
        <property type="protein sequence ID" value="ENSPNAP00000019483.2"/>
        <property type="gene ID" value="ENSPNAG00000026084.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429950"/>
    <dbReference type="CTD" id="4059"/>
    <dbReference type="GeneTree" id="ENSGT00940000161038"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF162">
        <property type="entry name" value="BASAL CELL ADHESION MOLECULE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043758522" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="137"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="250"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="340"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="425"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="568"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="570"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019483.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="600" mass="65529" checksum="82E91C68DB8814FA" modified="2024-11-27" version="2">MAGSSLRSLCLLFLSLGVCLAGITVKVSPEVDVILGETARLPCNYTISDSSVQPVVTWFIDSAGSRKRVAYRSPPDSGNDKIGLFKDRATMEADMTLVISKVNVGDEMSFFCQVRGGAVGVEEDVTKLRVFLSPEKPVISHNTHSITADQDQSSQVGTCTSRNGHPQPRIIWFKDSKPLPEVTDSKEKTYMVPRVVKESSGLFTVASELYMQLMKEDGKSVFHCTVEYAMPNGQITKESSDHFSLTVYYPAENMFFTLLNQGPFKEGDRVQMKCETDGNPQPEFEFYKGDVKLGQTGTGLWTLENVTRDDADIYKCYVLDFETFVELTRTLTLKVEYLDPVSIVPAGPLILKGGDALDLQCQTKSSNDHYVVWKKAGKELSQTGGLSLKSVTLADAGEYVCVASMRSVPGLEKQANITIRVEGKPEIDVPVKVEVKKKGDSVTLRCSALGYPAPQFNWRPSGKESVTVEGQKTISINILPATDAVITDGVTCEATNKFGTDTKKFEVAESANTADRRNHAGSGSPVFAKAEPQQAGSSIVVIAVVVSVLLLLFIVAVLYFLSKKNKMPCGKKDNAPGNGQDKHETKPFNEKDVEEAKSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDP0</accession>
    <name>A0A3B4DDP0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDP0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033029.2">
        <property type="protein sequence ID" value="ENSPNAP00000021600.1"/>
        <property type="gene ID" value="ENSPNAG00000028706.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271854"/>
    <dbReference type="OMA" id="LSHRWLY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15074">
        <property type="entry name" value="7tmA_Opsin5_neuropsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000219">
        <property type="entry name" value="Opsin 5-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002962">
        <property type="entry name" value="Peropsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01244">
        <property type="entry name" value="PEROPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="16"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="428" mass="48256" checksum="C1AD7EB70C4B7DBB" modified="2018-12-05" version="1">MFLSFFLSAGVCSLLGNSTLLYVSYKKKRYLKPAEFFIINLAVSDLGLTLSLYPLAIISSFSHRWLFGRVFCIMYAFCGVLFGICSLTTLTLLSLVCFVKVCYPLYGNRFTATHGRLLLVCAWAYALVFACSPLAHWGEYGPEPYGTACCIDWRLSNQRSSARSYMVVLFALCYLLPCVLIVASYTRILLTLHASRRTMEQHISAPTHLSNIHTIIIKLSVAVCIGFFAAWSPYAVVSMWAAFGQIKSIPPLAFAMPAMFAKSSTIYNPIVYLLLRPNFRRVMWRDLGLLQRACFRCCLCSTIPQRPCRSKTVPLQSLRRRLHKSHSVHGELPGSRAKGSMCERCGDAFECFKHYPKACHLETRDNGTSLRCPVAGTILPLTVGLKQQHKSTKKTSVRTRMWGKQRAEIEHFKISLETLPVHAKMAWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDP5</accession>
    <name>A0A3B4DDP5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CDCP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567754.1">
        <property type="nucleotide sequence ID" value="XM_017712265.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDP5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021181"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032258.2">
        <property type="protein sequence ID" value="ENSPNAP00000021181.1"/>
        <property type="gene ID" value="ENSPNAG00000028190.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436053"/>
    <dbReference type="CTD" id="200008"/>
    <dbReference type="GeneTree" id="ENSGT00940000158291"/>
    <dbReference type="OMA" id="QQNVQEY"/>
    <dbReference type="OrthoDB" id="10009301at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF47">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017218545" description="CUB domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="27"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="142"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="256"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="534" mass="59102" checksum="5881EA921B8218D5" modified="2018-12-05" version="1">MELRIAFGCLQLLYVFGKAASKKGVKCGGILSATSGNISSPNFPGLYPYNVECSWLIVVSEGSSVLLTFHHFELEYHIDCAYDYIKIYNGISEDEGNLLGKFCGDVSPPQFSSSWNVMSIIFRSDRHVTHRGFSVGYRKDMCGGVLTGLSGVISSPGYPLEYSNNADCSWTVHVSNRSVVSLVFLDFQLENNEGCNFDYIALFDGPTVKHRHIGNYCGNERPPNTVTTSNQLLVVFKSDFNIGGRGFKAYYYSGECQQILKAISGNFTSPNFPNIYPNNINCHWTVVLAAGYRIKLFFPVMELEDQNSLTDSCDFDSVVVYDGDSEADPLLGRWCGTEPPPSLTSKTNKLLVVLNTDRNSAFKGFTASYTGVVPVNVSCTRTEFQIQISQQSLPQLDRESVYLGNPSCSAQLTATTYKILARFVNCGTAGQKRRNITMLVNTLFIDFSDGKQQNVQEYEVQCDAQRKVAMVSIISVEERTRMNELARLAEASSSQGTGENSETEPHETSDIVFISVCVLAVILMLIAIVWLVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDS1</accession>
    <name>A0A3B4DDS1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Spermatid perinuclear RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDS1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034771.2">
        <property type="protein sequence ID" value="ENSPNAP00000022567.2"/>
        <property type="gene ID" value="ENSPNAG00000029975.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154687"/>
    <dbReference type="OMA" id="SILMIRI"/>
    <dbReference type="OrthoDB" id="8898434at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000001">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000003">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006561">
        <property type="entry name" value="DZF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049402">
        <property type="entry name" value="DZF_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049401">
        <property type="entry name" value="DZF_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762:SF1">
        <property type="entry name" value="SPERMATID PERINUCLEAR RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20965">
        <property type="entry name" value="DZF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
        <property type="entry name" value="DZF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00572">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51703">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DZF" evidence="8">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="7">
        <location>
            <begin position="351"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="423"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022567.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="54360" checksum="F8553F74D6DF4B45" modified="2024-11-27" version="2">MAKHSAIYPSAEELEAVQSLVSVAEGGLKQVSDWLANSAKASVVICKDQAEPSTKCGGLLVGVVRVGSVAKGLLIKGVMDLELVLLCREKPTRKLLLTICTNLPLQMKKLTEDSYTVQPCIPEAAILVFKSSRPDLPLRVTLTSMEMKMENTKAKDSSDLLDQQRCLLALASLRHAKWFQVRVSGKKSCLTVLRILRDICIKRPAWEPLKGWPLELICEKAVATSYRPLGPSEALQRVLGCISSGILLPGGPGLRDPCEKEQVDTLSAMSSDQAELLTHTAQHALRLLAFGQIHKFLQMDPLPSAKPAVEPKLAEGWYFHVWCQVLLVCYESSVVCFDFQGKSQGPVLTSRGKNPVMELNEKRRGLSYDLITENGENQQQRFIIEVQVDGLRFRGCGPNKKVAKANAALAAIERLFSAHNTNSNKKKRSYTKVNPATNGFHRGKRGVTMPRPVLPLPPPAPAYFTPGIFSQSTSTETFCRCTKKQKTNKQTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DF39</accession>
    <name>A0A3B4DF39_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-KFYI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">NADH-ubiquinone oxidoreductase KFYI subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFC1 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547436.1">
        <property type="nucleotide sequence ID" value="XM_017691947.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DF39"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022120"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041003.2">
        <property type="protein sequence ID" value="ENSPNAP00000022120.1"/>
        <property type="gene ID" value="ENSPNAG00000004579.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424132"/>
    <dbReference type="CTD" id="4717"/>
    <dbReference type="GeneTree" id="ENSGT00960000187553"/>
    <dbReference type="OMA" id="NEHQAEY"/>
    <dbReference type="OrthoDB" id="9900059at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026192">
        <property type="entry name" value="NDUFC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17097:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 SUBUNIT C1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17097">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE KFYI SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15088">
        <property type="entry name" value="NADH_dh_m_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="69" mass="7606" checksum="6E440E5C7BB2DB60" modified="2018-12-05" version="1">MPAGRLLLQTTALTKTISRNAFTAAKPDSSRPNMLRVGLAFGSTALLWALLFKQHSTDVQEYKTRNGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFX0</accession>
    <name>A0A3B4DFX0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial peptide methionine sulfoxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MSRA</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFX0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012991.2">
        <property type="protein sequence ID" value="ENSPNAP00000023302.1"/>
        <property type="gene ID" value="ENSPNAG00000000526.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165219"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000001">
        <property type="entry name" value="Peptide methionine sulfoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF23">
        <property type="entry name" value="PEPTIDE-METHIONINE (S)-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="10">
        <location>
            <begin position="66"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="223" mass="25084" checksum="CE59E65BEBD9D196" modified="2018-12-05" version="1">MVQLDRLRLIWRGFVHSRTGEMSSKMQMPPPERALPGRPDTLRVSAKHHVNGNRMVLPFPEGLQMAMFGMGCFWGAERKFWRQKGVYSTQMGYSGGYTPNPTYEEVCTGKTGHAEVVRVVFSPGKISYVHLLKVFWESHNPTQGMRQGNDMGTTYRSAIYAYTQEQLSQALASKEEYQKVLTEEGFGPITTEIDYHQQYLSKNPGGYCGLDGTGVSCPIGLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGM6</accession>
    <name>A0A3B4DGM6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Leucine zipper protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LUZP2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017563280.1">
        <property type="nucleotide sequence ID" value="XM_017707791.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGM6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022251"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034358.2">
        <property type="protein sequence ID" value="ENSPNAP00000022251.1"/>
        <property type="gene ID" value="ENSPNAG00000006803.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433293"/>
    <dbReference type="CTD" id="338645"/>
    <dbReference type="GeneTree" id="ENSGT00390000013180"/>
    <dbReference type="OMA" id="EGKTCSM"/>
    <dbReference type="OrthoDB" id="8767066at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026718">
        <property type="entry name" value="Luzp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22414">
        <property type="entry name" value="LEUCINE ZIPPER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22414:SF0">
        <property type="entry name" value="LEUCINE ZIPPER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017206385" description="Leucine zipper protein 2" evidence="3">
        <location>
            <begin position="18"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="207"/>
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    </feature>
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        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="39039" checksum="E998017F34D72350" modified="2018-12-05" version="1">MKFLGAVYVLLLLPALSFNSSYEVLEKKLKEVFTERTGILRQLSKTSKELDSIKGNLQSLKNDDTVPKKDVQRILELSHKQREEMKSLQAALQKQLDEAAERAEKQQATIKFLKMEMEKKTKIIKDLQQENKSLKNKLLSGNKLCDIHAEESKKIQAQLKELRYGKKDLIFKGQQLMDLENKLRVAKEELEKAALDKESQLKALKDTVHICFSSVLHSQTVNLHRLPATPTHLMRYSPLVNNSRVTFQQPHAKDIPKVPRITTSKLPVNVEIMRRDGAGVKDCQMVKVGAECFQNQTERTSVEISKTGHNQPRSPESSGHGSARTPADSGKTEGAPEKTQVDKHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DIE0</accession>
    <name>A0A3B4DIE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DIE0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014353.2">
        <property type="protein sequence ID" value="ENSPNAP00000022876.2"/>
        <property type="gene ID" value="ENSPNAG00000007294.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230358"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig_V-set"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022876.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="101" mass="11086" checksum="9E86B849CAF5D62C" modified="2024-11-27" version="2">QSMESLQTAVQKKPGETLSLSCRGSGFSFSCCSMQWVRQLAGKPLEWIGRVRASGIGNDYSKSFQGRTEITRDNSNSMVYLKLSGVTVEDSAVYYCAKSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPG8</accession>
    <name>A0A3B4DPG8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">One cut domain family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CUT homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPG8"/>
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        <property type="gene ID" value="ENSPNAG00000009842.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cut-like homeobox 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cut-like homeobox 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF4">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01109">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51042">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="15">
        <location>
            <begin position="50"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="177"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="179"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="144"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="247"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="371"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="387" mass="41974" checksum="5452EF1F08587A84" modified="2018-12-05" version="1">MSVKSLGEPLGLQASGHEQPQSSEPSEGSQPSTPLPLPLSTLGPTGLSIQEMVAMSPELDTYTITKKVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLSDPRNVEKLMDMKRMEKKAYMKRRHSSMSDTQPVESGLVGTDFGQSSSPQHHPQQQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAGAAASGEGGSPSVRPGKASEGDSCDGTEMDAVADDSKPPGSTLEEPSAAKAPTPSHSPTLRDPTTSAAEGPGTSGHQVPPQGSSSGLGLFGFPETAPSASSSTSRNPKDSNLRKKKAANLNNIIHRLEKAASKEDPSEWEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUJ1</accession>
    <name>A0A3B4DUJ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA decay factor PAT1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PATL2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PAT1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541816.2">
        <property type="nucleotide sequence ID" value="XM_017686327.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUJ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027133"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003385.2">
        <property type="protein sequence ID" value="ENSPNAP00000027133.2"/>
        <property type="gene ID" value="ENSPNAG00000002315.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413687"/>
    <dbReference type="GeneTree" id="ENSGT00520000055649"/>
    <dbReference type="OMA" id="YQRPFED"/>
    <dbReference type="OrthoDB" id="8251691at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019167">
        <property type="entry name" value="PAT1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF3">
        <property type="entry name" value="PROTEIN PAT1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="403"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="69"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027133.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="687" mass="77682" checksum="3052E567F1DC43EA" modified="2024-11-27" version="2">MSDSEHPEEKENSTAGPAWPENGSQWSDEGEMEESGLLQDMVEEDEEIDLYNEETFGLDLSAEDLHADPVDLLFVDGNQPPTPPSSPPPSQRQPSPPRPKPQIQPQAPAVHFKAASRRERPQRQLFDDPAVMRTVEGPPSLKSLDSAIVDCGRSSYWGDLNANTWMMPSYSSPRRTPASILQDQAIVGVIDKSTRGRMQYGSPNLRSPLHPVDQRRLPRVPVCPLLPSSPLHPQSPFSMKQRYNQSGVFLSPTPPRFHAPQALAPKMMQVRFGPNSPRPSPCYSPFSAPIQRFRLPVLATQLHPQHKRLLSQRPHGSRRKSRSWEHYAELMSAKEKEWIIKLQMMQLQSENPYQDDYYYQEYYRRMEAKLAEEEMLGDRSKREPPKLTTPYVTKTVSYAPVVHIEGSLGQVAVSTCYSPRRAIDAVHVHPPEEQKDTRQQRLEVLNTIEKCLMILLAVEEAERTKETVSDEEERRRLMKTVQRGVDEISSRLHSSSSLESMDEFLQCLLVSKGKRLLARLLPFLSQDAARHILTVITTHLPALMSRDVDEALPVLYPPLRTVISTLAFTQLVAVLQEFTSSLPDSKDSRLTLACQNKFGLSLLYALLSQGERLLTSDVPMEPSIGDFETWTDTVFHVASQLSRTSLVEPLFLPSNLLTLFCRYLDKRTVQQLKNNMESSAGHLAVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DYC5</accession>
    <name>A0A3B4DYC5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037387430.1">
        <property type="nucleotide sequence ID" value="XM_037531533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DYC5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028458"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014639.2">
        <property type="protein sequence ID" value="ENSPNAP00000028458.2"/>
        <property type="gene ID" value="ENSPNAG00000014161.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426211"/>
    <dbReference type="GeneTree" id="ENSGT01060000248852"/>
    <dbReference type="OMA" id="CTESIGH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045124">
        <property type="term" value="P:regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001204">
        <property type="term" value="P:regulation of osteoclast development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042836">
        <property type="entry name" value="SIG15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46942">
        <property type="entry name" value="SIALIC ACID-BINDING IG-LIKE LECTIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46942:SF1">
        <property type="entry name" value="SIALIC ACID-BINDING IG-LIKE LECTIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043467223" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="16"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="146"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028458.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="279" mass="31399" checksum="2B11EDA9128A731E" modified="2024-11-27" version="2">MKMQLVQVAFFLCCSPGLMADNKWSMTVPKAVEQNFGEDAIIPCSFTAPYQDYQGNLKAIWRTKSQVIFRCVSKGNPSESGQNCTESNGRYSLSGDPRRNNISLRIKNTLFMDAERYFCRVELNKQGGAYETSTGTILNIRGEQFVACDVKGTPPPTVTWIFPENINASLVSVQSSLSRASSTIPANLPNTNYTCQIHGKNRTQHLSIYFRGAQEQQQQIPFPFFLVVTILSAVFFIIFLVTLAVLYKKGSSEHVDTATQMKMKKTKYTEKDEDVYMNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZA0</accession>
    <name>A0A3B4DZA0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TCDD-inducible poly(ADP-ribose) polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TIPARP</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZA0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000232.2">
        <property type="protein sequence ID" value="ENSPNAP00000028334.1"/>
        <property type="gene ID" value="ENSPNAG00000013979.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437970"/>
    <dbReference type="GeneTree" id="ENSGT00940000155368"/>
    <dbReference type="OMA" id="IEEANCR"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
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        <property type="entry name" value="WWE-dom_subgr"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF7">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE TIPARP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PARP"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WWE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WWE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="7">
        <location>
            <begin position="204"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="7">
        <location>
            <begin position="289"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="8">
        <location>
            <begin position="408"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
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    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028334.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="616" mass="69736" checksum="AAA042D316556C77" modified="2018-12-05" version="1">MEKSLHVMPEPEGSDGEKVLEEATLSLAMEDGEFEQRLGLVDKIPLVKPYFKKKHTQRKLGAKCLRALQEPLLSTLLVAGEGVFVAPCAAAAAATIEKQATMNEVRTKGAGVSEAQTGDVAADVAADATSAPAVVASEALSDIFAPAEPLGGASAQEGEMFRDKSEEASLDLVFELLTQLQYHTHQGDDIDICADFLQGACLYGSDCERHHTVLPYHWQIRRSDTHVWQSISDDAQEQLERLYCNPDNEQVRLKFLSRVFMLDFSAMRVCDLEFDLVRRLSTASSSGATSSSTNPAPSCLTVWKYYCRDNFGWREYSEPVVRLIEEASGRGLKEVRFITLQNQYILNIREGFQQNAVFGFRRQIKKRPLFMSSVRLSSYLQTLGGVYALEVSGPQPLSLTSTTSPRPFPETWVPMSPDQDFVQVPVSRDDRSYRTVYNLFHKTVSETKFRILKILRVQNPFLWEKYKRKKEYMSRRMSEMDRLLNERHLFHGTSQDVVEGICKHNFDPRVCGKHATMFGQGSYFARKAVYSHNFSKRSARGVHYMFLAKVLTGRFTVGNPSMRRPPPLCPRDPSSDLFDSCVDNWMDPQIFVIFNDDQSYPYFIIQYEEVASTVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZE5</accession>
    <name>A0A3B4DZE5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZE5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017204.2">
        <property type="protein sequence ID" value="ENSPNAP00000029782.2"/>
        <property type="gene ID" value="ENSPNAG00000016104.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154435"/>
    <dbReference type="OMA" id="ACFLMVS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF38">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029782.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="222" mass="24668" checksum="19D4663620B26230" modified="2024-11-27" version="2">MHSTGHPVSAAVFNCPPLSVDCLLWLHLPALIVSGMSTNNNYIVKDAAGQQMFFAAEENDSCNRMCCGSSRSFTISLTDNSRREVFTLERSLNCSSCCCPCCLQELEVHAPRGKPIGYVTQNWHPCLPKFTILNERRKPVLKIDGQCCMGSCGSQVYFKIKTLDTSSLVGAIFKQPTDIVQERFTNADNYGLQFPVDLDVKIKATVLGACFLIVRHFFVQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3J3</accession>
    <name>A0A3B4E3J3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Interleukin-7 receptor subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IL7R</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017574420.1">
        <property type="nucleotide sequence ID" value="XM_017718931.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3J3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029886"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017422.2">
        <property type="protein sequence ID" value="ENSPNAP00000029886.1"/>
        <property type="gene ID" value="ENSPNAG00000016278.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440174"/>
    <dbReference type="CTD" id="3575"/>
    <dbReference type="GeneTree" id="ENSGT01120000272790"/>
    <dbReference type="OMA" id="LRWRTHI"/>
    <dbReference type="OrthoDB" id="8611929at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022010">
        <property type="term" value="P:central nervous system myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022011">
        <property type="term" value="P:myelination in peripheral nervous system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003407">
        <property type="term" value="P:neural retina development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046427">
        <property type="term" value="P:positive regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033077">
        <property type="term" value="P:T cell differentiation in thymus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048538">
        <property type="term" value="P:thymus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007632">
        <property type="term" value="P:visual behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF27">
        <property type="entry name" value="INTERLEUKIN-7 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017293902" description="Interleukin-7 receptor subunit alpha" evidence="8">
        <location>
            <begin position="20"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="43880" checksum="5699A25B7D40D7BA" modified="2018-12-05" version="1">MASCVLWIFITLCPFLSYSESGDDASPDAESSVFCSSMISLDMNSLICGLIDDAAEIESATLCKTGMKNKQTSCINGTLVKDTISFQKLEILQRYELWTHFKDGVKESQNIDLVKIVKIPTPEVYNATYNFDEAIIYIGYKHDLVVNPNFQLQVRGTSGTDKMTMTINYQQVTIGEDKLRNSKTYYVRVRAKPVDYFDGSWTAWSQEKYFSRTPAEDPSMVTYVILCTSLILVVGIALMILRWKKEIQAYISPNIPNPKATLAQIHREKEHPPVSFSPEIFNDVSINRVDYAEEKQLGPEGDEDQEDATGTCWSEAEGLRCSQPLSLCDKQNGDERRLEEEMSHLKIKLLDQAEEVENEDVCQSLATLQRDCKDESYVTMSSLYKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3W3</accession>
    <name>A0A3B4E3W3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Golgi transport 1Bb</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in fusion of ER-derived transport vesicles with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3W3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017495.2">
        <property type="protein sequence ID" value="ENSPNAP00000029924.2"/>
        <property type="gene ID" value="ENSPNAG00000016346.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014507"/>
    <dbReference type="OMA" id="MWLTDAQ"/>
    <dbReference type="OrthoDB" id="204784at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF250">
        <property type="entry name" value="GOLGI TRANSPORT 1BA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029924.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15739" checksum="D10E058A861A3C80" modified="2024-11-27" version="2">MGLTGFGVFFLFFGMILFFDKALLAIGNILFVVGLAFVIGLERTFRFFFQKHKMKATSFFLGGVFVVLIGWPIIGVVLEFYGFFLLFRGFFPVVIGFIRRIPVLGSLLNLPFVSGVSVWTDSNQIMGKLQVYRSQTVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8H8</accession>
    <name>A0A3B4E8H8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KLHL29</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8H8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031564"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020677.2">
        <property type="protein sequence ID" value="ENSPNAP00000031564.1"/>
        <property type="gene ID" value="ENSPNAG00000019010.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411895"/>
    <dbReference type="GeneTree" id="ENSGT00940000158824"/>
    <dbReference type="OMA" id="SHSYGMN"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd18468">
        <property type="entry name" value="BACK_KLHL29_KBTBD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF17">
        <property type="entry name" value="KELCH-LIKE PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="331"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="262"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="875" mass="94784" checksum="AECD6172004B0BEE" modified="2018-12-05" version="1">MSRHHHRFERDYRGPWDRRDRPSAVHNGVANNACSPAAPPLANGNNRPGLLPLPVIPSLLPSPVTIAVTTSSTDLGAKRASAASVAAPAVKAAGLSPGENQTPGLLLAPGVRWGQTPINQSTPWETDEPPAKQHRESDATGPPWVPPVVAVSQPSLLAHSYGMPQPPAYSQGVSVQSPVIGVTPAIQTPVPPHHPLMTAHFQVTPHSSQPPGGLVLSLPNQSPYATSQPPITPSPLTTGGQVAHPTPQSVVGNGHLTHPLMQPPALPSAALPLTNGQTTPQPPPASANQESPNGLQMLRTVGMGKYEFTDSGHPKEMLKELNQQRKEKEFTDLKIIVEGKEFEVHQNVLASCSLYFKDLVKRSLGETGSGEKLELKMTNISADVLDMLLEFVYTGSLVIDSANAKTLLEAANKFQFNTFCKVCVSFLEKQLTAGNCLGVLAMAEAMCCTELHNMAKAFALQNFPEVAGQDEILSISKEDLVSYLSNDSLNTKAEELVYETVIKWIKRDPTNRVQHISELLAVVRLPFIHPSYLLNVVDNEELIKSSEACRDLVNEAKRYHMLPHARQEMQTPRTRPRLSAGVAEVIVLVGGRQAIGMNQRSLTAVTCFNPQNSKWYPLASLPFYDREFFSVISAGDNIYLSGGTESGVTVADVWCYMSLLDNWNLVSRMTVPRCRHNSLVYDGKLYTIGGQGVAGNLDHVERYDTITNQWETVSPLPKPVHSAAATVCGGKVYVFGGVNEAGRSAGVLQSYVPQTNTWSFIESPMIDNKYAPAVSLNGFIFILGGAYARATTIYDPDKGNIKAGPNMNHSRQFCSAVILDGKIYATGGIVSSEGPALGNMETFDPCTNTWTLLQNLPCPLFRHGCVVIKKYIQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8Y9</accession>
    <name>A0A3B4E8Y9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Adhesion G protein-coupled receptor G11</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017562427.1">
        <property type="nucleotide sequence ID" value="XM_017706938.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8Y9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023551.2">
        <property type="protein sequence ID" value="ENSPNAP00000033042.1"/>
        <property type="gene ID" value="ENSPNAG00000021438.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432831"/>
    <dbReference type="GeneTree" id="ENSGT00940000155621"/>
    <dbReference type="OrthoDB" id="8951579at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF474">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017333250" evidence="7">
        <location>
            <begin position="31"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="510"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="544"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="613"/>
            <end position="633"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="667"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="717"/>
            <end position="739"/>
        </location>
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        <location>
            <begin position="745"/>
            <end position="764"/>
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        <location>
            <begin position="349"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
        <location>
            <begin position="508"/>
            <end position="767"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="796" mass="88129" checksum="17BD2D5FA39EF667" modified="2018-12-05" version="1">MKWMAGVKSGPYRSVVVLAVILGMSSLSLSKSCKMDKSCTSIVVIDGNYTGPMEMTKSTCPNASGPSQSDKVCFFSFNMFENLKQELAEALKNSSTSYTTPTLTVSTSKTTTKMGSKANGGESWLSWVWSWLLSTSSQNKSPQTSAQAGPNILYEYSASIKNILEEINSTDKVNNCTYEEIGMWLLKTNNTYQLRITRVENIRPKAHICFVKGKCNNTWQQWIGGGFTRCVGPNNSCQTKGCNVICLDPTTFCNNAEYTSNCDGCTSNDDIIEFKDTAPVCSKTGTPVLQPDLQLKMNTTAIPTETVSEEIKSLTSSVLQMMGDKTTASVTVGDAQGFFVKPKDRSKLEAVSFIYSENTDLSIIQDKTQTEKYPTVISIPKEAFEKAANIDNITLFTSVFRFPNFTKDAKNSTVLNNEVYSIDMGTEITNLTNTISFIFRSVKKQKDVSPHCNSWDGNGSKPNWITDGCGTTETGDNVTCECVHLTFFAVLMVPNPTETISSTDLSNLTYITSIGCGLSIFFLGVALFMHFLLRRGRQSDSVHILINLFVSLVLLNLTFLTNEYVANTGNLIGCKLMAGFMHYCLLTSFTWFGLEALHLCLQLGRNSTPIKHYLTKICIAGWAPAAVVVTVVFCLQKYNQLVIHADNGKDVKMCWITDSAVHYVVNIGYYSVIFIFTFTTFVIMLRWIFLLKGTAANRHEPLAGSGKRTTTGTSDALTVMGLCCTLGLTWGFAFFAYGALRLPSYYIFTILNSFQGFFLFIYYYKTSKFVGDQMTSEKTSAITEATSVENPYEKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAS9</accession>
    <name>A0A3B4EAS9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium channel regulatory subunit beta-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">A voltage-gated sodium (Nav) channel consists of an ion-conducting pore-forming alpha subunit functional on its own that is regulated by one or more beta subunits. Forms homodimers and homotrimers. SCN3B is non-covalently associated with alpha subunits and induces the formation of alpha subunit oligomers, including trimers. Interacts with SCN5A/Nav1.5; regulatory subunit of SCN5A/Nav1.5. Interacts with SCN7A/Nav2.1; probable regulatory subunit of SCN7A/Nav2.1. Interacts with SCN10A; regulatory subunit of SCN10A/Nav1.8. Interacts with NFASC; probably involved in targeting the sodium channels to the nodes of Ranvier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569924.1">
        <property type="nucleotide sequence ID" value="XM_017714435.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000032930"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023347.2">
        <property type="protein sequence ID" value="ENSPNAP00000032930.1"/>
        <property type="gene ID" value="ENSPNAG00000021213.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437383"/>
    <dbReference type="CTD" id="555423"/>
    <dbReference type="GeneTree" id="ENSGT00390000018560"/>
    <dbReference type="OMA" id="DNCAGEQ"/>
    <dbReference type="OrthoDB" id="8868224at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086002">
        <property type="term" value="P:cardiac muscle cell action potential involved in contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086091">
        <property type="term" value="P:regulation of heart rate by cardiac conduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000375">
        <property type="entry name" value="Sodium channel beta 1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027098">
        <property type="entry name" value="Na_channel_b1/b3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10546">
        <property type="entry name" value="SODIUM CHANNEL SUBUNIT BETA-1 AND 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10546:SF9">
        <property type="entry name" value="SODIUM CHANNEL SUBUNIT BETA-1 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017277750" description="Sodium channel regulatory subunit beta-3" evidence="21">
        <location>
            <begin position="26"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="45"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="221" mass="24523" checksum="4FAF737D9107026F" modified="2018-12-05" version="1">MSAVRLLWASLVLAALNVLLSDGACVEVDSDTEAVVGKGFKLGCISCKMRGEVEASATVDWWFMAKGESDFTHIYSYADMVGTVTDGRFSDRLDWNGSKKTLDLQDGSIYILNVTFNDTGTYRCYFDRTLTYDYYEFRTNTTKYISMNVVAKATRGMASILSEVMMYVSIIGLQLWLLVEMVYCYRKIAAAGEEALRESAAEYLAITSESKDNCAGVAVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EBT8</accession>
    <name>A0A3B4EBT8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EBT8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032696"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037491.2">
        <property type="protein sequence ID" value="ENSPNAP00000032696.2"/>
        <property type="gene ID" value="ENSPNAG00000020809.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156528"/>
    <dbReference type="OMA" id="QPKITHA"/>
    <dbReference type="OrthoDB" id="1022360at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000073">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF3">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="10">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="403"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="230"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032696.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="677" mass="75273" checksum="C0BCC20AA7684B29" modified="2024-11-27" version="2">MEGALRMFQRKKKKRKIEISAPKNFEHRVHTSFDAVRGCYVGLPPQWQSVIETLRRPKPVVDPSSITPVRLKQEKTIVRGSFIGHGDYIAAAIAEMTRLSVTSSNSLRRTSPSLRKRAQSMGRLGEVAEGDTYQYQELCKNRQTNGQPALDWHERARQVYSESGSPRPGSKKSATFQPNGLPPRAKSIIEVSTTSSEQPRLMPRDILCSPGADQTEMFSHSERLRLHQPAVTHRDSKTNPRPVSCIYNTPTLNQGSKVSNPSERMTPSPELPVVSVDTPRRPYSTYDLKTNSCGFSAMSKPNGTSSPKAGHGTHSHPPLQSSSSYTIGFSPIKASVPPIQNSPSQPRPSPTGSPATNLPGASSPCLRTSQPATINCEQPKITHAQFKAALQMVVDKGDPRTYLENFVKIGEGSTGVVCIAREKHSGKQVAVKMMDLRKQQRRELLFNEVVIMRDYRHKNVVEMYKSALVEEELWVIMEYLQGGALTNIVSETRLTEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRIKLSDFGFCAQISKDIPKRKSLVGTPYWMAPEVISKTPYGTEVDVWSLGIMVVEMVDGEPPYFSDTPVAAMKRLRDEPAPSVRNSSKISPVLRDFLDSMLTRDPLQRASASDLLKHPFLLQSGSPRCLVPLVEQYRKRMSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECQ9</accession>
    <name>A0A3B4ECQ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aggrecan core protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cartilage-specific proteoglycan core protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the HAPLN family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aggrecan/versican proteoglycan family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017545692.1">
        <property type="nucleotide sequence ID" value="XM_017690203.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000033483"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024450.2">
        <property type="protein sequence ID" value="ENSPNAP00000033483.1"/>
        <property type="gene ID" value="ENSPNAG00000022144.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108418869"/>
    <dbReference type="CTD" id="559593"/>
    <dbReference type="GeneTree" id="ENSGT00940000155971"/>
    <dbReference type="OMA" id="EDWIVTQ"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03517">
        <property type="entry name" value="Link_domain_CSPGs_modules_1_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03520">
        <property type="entry name" value="Link_domain_CSPGs_modules_2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000009">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000011">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000001">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF54">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0373">Hyaluronic acid</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="17" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017481781" description="Aggrecan core protein" evidence="20">
        <location>
            <begin position="17"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="23">
        <location>
            <begin position="35"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="25">
        <location>
            <begin position="154"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="25">
        <location>
            <begin position="254"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="25">
        <location>
            <begin position="470"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="25">
        <location>
            <begin position="571"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="1222"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="22">
        <location>
            <begin position="1270"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="24">
        <location>
            <begin position="1388"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="700"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="933"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1069"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1129"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="724"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="823"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="834"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="933"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="614"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1226"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1247"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1390"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1419"/>
            <end position="1446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1476" mass="157567" checksum="CBE47E9B40E88FDB" modified="2018-12-05" version="1">MTALLLLCVCLSVVSASVLFGDMDDQDSTLSVSIPVEQPLRPLLGGKMVIPCYFQDNTVHDPGAPTIAPLSHRIKWSYIHKGQISLVLVATGGVAHIETDYLDRVTMVNYPTMPTDVTIEITELRSKDSGTYRCEVMHGIENNYDSVEMQVQGIVFHYRAITSRYTLTFEEAKAACIQNSAVIATPEQLQAAYDDGFHQCDAGWLSDRTVRYPIHEPREPCYGDKENFPGVRTYGIRDDNETYDAYCFAEKMSGRVFYSMSFEKFTFAEASEQCAKLGAHLATTGQLYLAWKAGMDVCNAGWLADRSVRYPINVARPQCGGGLLGVRTVYLFPNQTGYPLPESRYDAICYEGEEEVPSPPTAGPEVSSPLTLVPEVDITTEPDFSVGTITFRPDAYPENVTSEVEMQGELVTQEPWSTTTIEAPLPLPPSIIGNITEDVEDVIIVATAQPGLGREQPRDNVSTVSESKGIVFHYHSDSKHYAYTFEEAQRACQQLGAEIATPEQLQAAYEPGLHQCSAGWLKDQSVRYPIVHPQYKCSGDQGDAPGVRSYGVQPAFKRYDVYCYMDQIKGEVFHVSSLVGFTYYEATAHCRKQNALLASTGELYAAWSQGFHKCSPGWLSDRSVRYPVQTPQFGCGVNKTVHTIYTNPDQTGFPDPYSRHDAYCFRANLSMIMTEDMLNATGMDFNLTHIKELIRPARPLVPPKSEEETGSGSAPDVSGSGIHSGDVSGSGIPSGDVSGSGIPSGDISGSGIPSGEISGSGIPSGDISGSGIPSGDISGSGIPSGDISGSGIPSGDISGSGIPSGDISDGGIPSGDVSGSGFPSGNISSSGFPSGDVPGSGISSGDVSGSGIASGDVSGSGISSGDVSGSGIFSEDVSGSGISSGDVSGIIVSFEGSGYMLSAEGSVSGGLQEAGKESALIFHLASGDLSGDLSGSGDISGSGQDRSGSGDLPSGSGSGDMITLIDHKQIDISSSHAKKEQELGTGHIEVSGFSGLHSGDIMSGSGLSGFQSGDVMSGSGISGFQSGDVSGSGASGFQSGDIISGSGFFGVSFVDSDMIDLTTRPVGEQEVSGISGEMSGDVSGFPSEDASGMSGLPSHPSGENFRSTSESGEVILLTEDEVIEVTSRPIESTQQGRGTLELSGEGSSPVVHTEEFFTILPSGSVLEEDISGRDLKEVNGTEKSLINTTSTTQTTLTYTTSPSILLQTPATVEQPTVTDATDPNPCDPNPCGDGSCFVQDGTAACLCPAGFTGENCATSVHGCEEGWVEFMGSCYIHFNEREMWTGAEQYCQELNAHLVSITSQQEQNFVNTLAQDYQWIGLNDRDKQNEFRWTDGSSLQYENWRPNQPDDYSNSGEDCVVMIWHEYGQWNDVPCNYHLPFTCKSGSVNCRTPPEVENARILGTKRDHYPVNSIIRYQCYTGFTQRHLSVIRCMPDGQWEEPKVECVGSNTNNRLRKRSLKWHSKAVSSRTWRKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EG17</accession>
    <name>A0A3B4EG17_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PCDH12</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017571626.1">
        <property type="nucleotide sequence ID" value="XM_017716137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EG17"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038421.2">
        <property type="protein sequence ID" value="ENSPNAP00000034239.1"/>
        <property type="gene ID" value="ENSPNAG00000023365.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438370"/>
    <dbReference type="CTD" id="51294"/>
    <dbReference type="GeneTree" id="ENSGT00940000160403"/>
    <dbReference type="OMA" id="ALFMSIC"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000003">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF42">
        <property type="entry name" value="PROTOCADHERIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017392999" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="21"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="717"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="26"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="135"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="243"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="356"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="462"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="617"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="759"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="975"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="788"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1156" mass="127783" checksum="0FC37CC5DB1DF35B" modified="2018-12-05" version="1">MLCFWVLFVAVLTVCPFCEAQYLDHAPLIIQYRVLEEQPAGTRVGRLLDDLRQRGETGSLEDFRVVEHGQALPFSVATRDGVVSTLGELDREKLCRGADHCELDFSVLYRKGGAVHFLRVRVEVMDLNDHSPVFPNPMQEVEISETVGLRTRIPLDRAVDPDAGPNGLQTYYLSPNHHFALDVRSSGGTKQAELVVIKELDRETQESFELTLEAWDKGSPPKSGSTKVKVIILDSNDNSPVFEESTPIVELPEDTLQGTEVINLHATDPDQGANGEVEYSLSKHSPEDVQRLFSVDPETGTVVLKGPLDFEQKISYEIDIQAKDRGHNAIPSHCKLQIRLQDVNDNAPRIHVTWTPPDSPVATVSEGASNETFLALVMVSDADSGPNGKVQAHIQDGSGPFRLKQIHADNYMIVTNGNLDREQQMEYNLTLLAQDSGVPSLSCVRHLTVHVLDENDNAPVFSKTHYKGVFKENNAPGFQVLTLKAHDVDLDLSGKVSYSIRESNELETPTASFFVHQTSGAVIVLKSLDYEESPKYSFIVDAIDHGFPPLTSSATVLIEVEDANDNYPIIKEPLPKKGVAVISVPVNTEKGEVVTELGDKVHRNLHPLSNRSFHHRPEGFLATTIVASDRDSGLNGKLQFRIIDGNPSGLFWLDETTGQLYVNSTNATELIGKVFKVAIEVSDMGTPALVTQTTMEVTFINLRDHLKNSSPGHQAQFSFTMLLAICLGGTCLLLLLTVALVTTFCRPEKRDNHSYNCREAESTYTSHPRRPQKHIRKTDIQLVPVLRGHRDDPPEEEVHPLSSGSPLPEDPPPQGQLSLSPTLARMARSQAYPDNERILQLTQGKTLRKPGNIELDCPHSPTMSYRTLRKARNPSLSSSSYSQTSTLRRHKLEDIITPIDSSETVAHGPFTQTTLRKPKNSERVEGMEEQDHRQILRNLVRLSMAAFGENSIELSSASPEVQQVSQLLSLLHQGQLQPRPNFRGNKYSHRTGRSGMQDADWLSTKDSGHGESEPGDVDWEAGRDSPVDQLLEEGLNNPDDVFSDFPDPAWMARLSLPLTSDYHENLYVPDGLPPPDCPNSTLGSLDDSTSFSTFGKTQEKPGSLGGEALLSEVSTLFEMLLTQKADAQPRTPADVLYRLSAAYRRSLGMDAPSGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EG41</accession>
    <name>A0A3B4EG41_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017537027.1">
        <property type="nucleotide sequence ID" value="XM_017681538.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EG41"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034708"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026811.2">
        <property type="protein sequence ID" value="ENSPNAP00000034708.2"/>
        <property type="gene ID" value="ENSPNAG00000024186.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410455"/>
    <dbReference type="GeneTree" id="ENSGT00940000163304"/>
    <dbReference type="OMA" id="CALPIGC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050918">
        <property type="entry name" value="CNF-like_PLA2_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914:SF9">
        <property type="entry name" value="COILED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043433680" description="UPAR/Ly6 domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="9">
        <location>
            <begin position="20"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="9">
        <location>
            <begin position="111"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034708.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="205" mass="21382" checksum="6B73903BA109D910" modified="2024-11-27" version="2">MRLQITLSLICVLFPKALTLKCYQYKTGPPGTCATTQTDCPDQCTTGTITVVTDGQQQQVSQKTCGGAAECFTGSINLGVWKTSFNLKCCSTDLCNGHTVEALPQESANGHKCYICDGDDCTGTVSCEGDEDRCVITTATAGDSQKMMKGCASSKLCTAGVSALQIAGVTGSVSCCEGNLCNSAEGVKLSLLIMLVPLISSILFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EI71</accession>
    <name>A0A3B4EI71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-dependent kinase inhibitor 1B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cyclin-dependent kinase inhibitor p27</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">p27Kip1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556681.1">
        <property type="nucleotide sequence ID" value="XM_017701192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EI71"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034994"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027343.2">
        <property type="protein sequence ID" value="ENSPNAP00000034994.1"/>
        <property type="gene ID" value="ENSPNAG00000003832.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429455"/>
    <dbReference type="CTD" id="368329"/>
    <dbReference type="GeneTree" id="ENSGT00940000159852"/>
    <dbReference type="OMA" id="THLRDQK"/>
    <dbReference type="OrthoDB" id="6373236at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265:SF9">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="17">
        <location>
            <begin position="31"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="92"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="175" mass="19864" checksum="4E6964481F74D80D" modified="2018-12-05" version="1">MSNVRLSNGSPTLERMDARLSDQPKPCACRNLFGPVDHEELTRELKGHLRAMEEASTETWNFDFATYTPLAHGRYLWTAVDTTDLPSFYSSTGNKSVDVNGNHERLAERSESQTERREQQHAAKGQRKRPACHDSSCQSKRSHTCVEEVSRHPPALAQQLDHHTPVKSSPQRRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EIR7</accession>
    <name>A0A3B4EIR7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acrosin</fullName>
            <ecNumber evidence="2">3.4.21.10</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.10"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.21.10" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EIR7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039037.2">
        <property type="protein sequence ID" value="ENSPNAP00000035204.2"/>
        <property type="gene ID" value="ENSPNAG00000025077.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165418"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF8">
        <property type="entry name" value="ACROSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="53"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035204.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="316" mass="35119" checksum="FBDBF335543098F3" modified="2024-11-27" version="2">HRHIMTHYDRIACFLYFDSCASHEILVVLIRPFTVFLGCGERPVMGPPSGSRVVGGHFAVKGAWPWQVSIQWMSSHICGGSILNHLWILSAAHCFNKSYSTLQVVAGLHSLSKIGSTAQIRSVERIIKHEDYDETSFENDIALLQLQHPLFFTTHVQPVCTLRNEREERDLSFSSCFVSGWGSTVFKGSLVDTLREAEVELIDTETCNQQDWHSGHVSDNMVCAGRERGGTDTCQGDSGGPLSCYSEVTGRFYIYGVTSHGEDCGLPKKSGLYTRASRYSAWLRIEERSASSGTGTKPTILHAIILSLLCQVWTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJP5</accession>
    <name>A0A3B4EJP5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dipeptidyl-peptidase IV</fullName>
            <ecNumber evidence="3">3.4.14.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DPP9</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.</text>
            <dbReference type="EC" id="3.4.14.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.5" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017561276.1">
        <property type="nucleotide sequence ID" value="XM_017705787.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017561277.1">
        <property type="nucleotide sequence ID" value="XM_017705788.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJP5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036677"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031593.2">
        <property type="protein sequence ID" value="ENSPNAP00000036677.2"/>
        <property type="gene ID" value="ENSPNAG00000004254.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432139"/>
    <dbReference type="CTD" id="91039"/>
    <dbReference type="GeneTree" id="ENSGT00940000158174"/>
    <dbReference type="OMA" id="VTHMTPQ"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.140.10.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 8-like isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000016">
        <property type="entry name" value="Dipeptidyl peptidase 8-like isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045785">
        <property type="entry name" value="Dpp_8/9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF193">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19520">
        <property type="entry name" value="Dpp_8_9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Dipeptidyl peptidase 8 /9 ,N-terminal" evidence="10">
        <location>
            <begin position="33"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="9">
        <location>
            <begin position="184"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="685"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036677.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="885" mass="101062" checksum="7015A83C68928127" modified="2024-11-27" version="2">MYRVKRVKLEDETEGSWKSLAAVKMTAVDDLSDSTEVVEMEDVPSQFFVEKHSWDGLREIIHGSRKYSGMIINKAPHDFQFVQKHDDSGPHSHRLYYLGMPYGSRENSLLYSEIPKKIRKEALLVLSWKQMLDHFQATPHHGVYSREEELLRERKRLGVFGITSYDYHAQSGLFLFQASNSLFYCHDGGHNGFIQAAPMKPVEIKTQCSGIRMDPKICPGDPSFIAFINNNDLWVTNIESGEERRLTFCHKGLNNIKDDPKSAGVATFVIQEEFDRFTGYWWCPTVSEDADGGKTLQLLYEEVDESEVEIIHVPSPALEERKADVYRYPRTGSKNPQISLKLVEIRTDEDGEIVSTQIKELVLPFKTLFPGVEYIARAGWTRDGKYAWAVLLDRSQQKLQLVLIPPALFLRVGMEDPQWEEHVAAMPEGVQPFIIHEEVTDIWINVHDIFYPFNQTNNDEITFLWVNESKTGFCHLYRVTSLLQPGCHQWTRDYTPSEDDFKCPIKEEVTLTSGEWEVLARHGSKIWVNEDTKLVYFQGTKDTPLEHHLYVASYESPGDIVRLTKSGFSHSCSVSQNFDMFISHYSNVSTPPCVHVYKLVGSDSDPLHKEPEFWASMMEAAGCPADYVPPEIFSFPAKSGFELYGMLYKPHNLKPGKKHPAILFVYGGPQVQLVNNSYKGVKYLRLNTLASLGYAVVVIDGRGSCQRGLKFEGALKNKMGQVEIEDQVEGLQYVADKYKFIDLSRVAIHGWSYGGFLSLMGLIHRPNVFKVAVAGAPVTVWMAYDTGYTERYMDVPENNLLGYEAGSVALHVDKLPNEPNRLLILHGFLDENVHFFHTNFLVSQLIRAGKPYQLQIYPNERHSIRCPESGEHYEITLLYFLQQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK29</accession>
    <name>A0A3B4EK29_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK29"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036812"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031941.2">
        <property type="protein sequence ID" value="ENSPNAP00000036812.1"/>
        <property type="gene ID" value="ENSPNAG00000027961.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164940"/>
    <dbReference type="OMA" id="DCPEMQS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF107">
        <property type="entry name" value="CRYGMX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="10"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="49"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="97"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="183" mass="21609" checksum="0FF79903A2873614" modified="2018-12-05" version="1">MSTSLHCPLLQITFFEDRNFQGRHYECTGDCVEMQSHLSRCNSIRVESGCWMAYEKPNYAGYQYMLHKGEYPDYQHWAGFNDCIRSCRMVPPYTGNYRMKIFERSDFAGQAMELMEDCPDLRERFHNGDVSSANVMEGFWILHEHPNYSGRQYFLRPGEYRRFNEWGSPSPAMGSLKRITDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK80</accession>
    <name>A0A3B4EK80_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FXYD domain-containing ion transport regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FXYD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564250.1">
        <property type="nucleotide sequence ID" value="XM_017708761.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK80"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036862"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032052.2">
        <property type="protein sequence ID" value="ENSPNAP00000036862.1"/>
        <property type="gene ID" value="ENSPNAG00000028040.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433901"/>
    <dbReference type="CTD" id="5349"/>
    <dbReference type="GeneTree" id="ENSGT01150000287666"/>
    <dbReference type="OMA" id="CRCKFKQ"/>
    <dbReference type="OrthoDB" id="9888529at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017080">
        <property type="term" value="F:sodium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043269">
        <property type="term" value="P:regulation of monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20328">
        <property type="entry name" value="FXYD3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.780:FF:000008">
        <property type="entry name" value="FXYD domain-containing ion transport regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.780">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000272">
        <property type="entry name" value="Ion-transport_regulator_FXYD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14132:SF14">
        <property type="entry name" value="FXYD DOMAIN-CONTAINING ION TRANSPORT REGULATOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14132">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02038">
        <property type="entry name" value="ATP1G1_PLM_MAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017105424" description="FXYD domain-containing ion transport regulator" evidence="7">
        <location>
            <begin position="19"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="90" mass="10078" checksum="08EC7E3503559FE9" modified="2018-12-05" version="1">MARILAMVFVTFVSLVLAEDQQTEDDPFTFDYHRLRVGGLILAAVLCLIGITILLSGHCRCKFNQDKRRRTGSNTATQQMLNDTARASEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELV8</accession>
    <name>A0A3B4ELV8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 167</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELV8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032147.2">
        <property type="protein sequence ID" value="ENSPNAP00000036895.2"/>
        <property type="gene ID" value="ENSPNAG00000028110.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010210"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028194">
        <property type="entry name" value="CC167"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31759">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31759:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15188">
        <property type="entry name" value="CCDC-167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="201"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036895.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="283" mass="31544" checksum="81E29710AFBE9C60" modified="2024-11-27" version="2">MAEASASGTSTQSGAKNLDAVENALLPEDHSKNHFDIIQLQEKEVRLLGRAESVDSAEMEVQSSTLSDKELMEMRADFDASVSPVLSLEESDLEPPEVGELLMCVEEPEVEEIMDNVPLTTLTSATPLPGSPAFISAPEIILGALHYLPCPQELPPFSMKAPGPAESYMSSKSESTDEASGSQLSSDSPFEEAPLKAQLQIDRIEEQRSRCQSSLERAEFRHRREQLLDEDRQALEDEMTIMNERIQKYEKELEVLRGENRRNMLLSVALLAISALFYYAFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EPR1</accession>
    <name>A0A3B4EPR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RUN domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RUNDC1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May play a role as p53/TP53 inhibitor and thus may have oncogenic activity.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565875.1">
        <property type="nucleotide sequence ID" value="XM_017710386.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EPR1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037768"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034599.2">
        <property type="protein sequence ID" value="ENSPNAP00000037768.2"/>
        <property type="gene ID" value="ENSPNAG00000029796.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434936"/>
    <dbReference type="CTD" id="146923"/>
    <dbReference type="GeneTree" id="ENSGT00390000007311"/>
    <dbReference type="OMA" id="THKGNHW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd17683">
        <property type="entry name" value="RUN_RUNDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000012">
        <property type="entry name" value="RUN domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058732">
        <property type="entry name" value="RUNDC1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047343">
        <property type="entry name" value="RUSC1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15591">
        <property type="entry name" value="RUN AND SH3 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15591:SF19">
        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26030">
        <property type="entry name" value="RUNDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="7">
        <location>
            <begin position="377"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00055063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037768.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="569" mass="63627" checksum="B68B423C6C6407C3" modified="2024-11-27" version="2">MSTEDLSTSDSEAPGAAAGERWAPVGAVANPEEEEWSSGRVGTEPPASTELEPCSPASKLRRLEDEREQLRAALLAVSSHFAQVQFRLQQVARAPADERERTRMLQELEEFAFRGCPHVLGHRGRENPSEREQRERLEAQREKQKELIFQLKTQLDDLERFAYQEGSYDSLPQSVVMERQRVIIDELIKKLDVNLNEDIGKLTPEELRQRVDAAIAQIVNPVRVKEQLVEQLKTQIRDLEMFINFIQDEVGNPLLSEGESSQQARASGPKPRSQGGMKQVDPEQAQRLRETGLQLIQKALAVLQIFALSQFGCAASRMPQSVWSHGDGAQDYGPLLQKLEGAVERVRLQASQRQPAGLEEHVVSYTSGLTLGGPGDELTASVRKDLAVALRDLLAHGLYTPSQGMSLVLAPISCLLPFSPSPQTLHPWELFVKYYHSKNGQAFVESPARQLSQSFSLPLGGSKVTITPKQSLLWAIHSVLREHGRYKRSADSEFKALVCMALNEQRLVSWVNLLCKSGTLVHSHYQPWSYMAQTGFEGALRILGRLSHLKFNLPVDLAVRQLKNIKDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IIE3</accession>
    <name>A0AAR2IIE3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcription factor involved in specification of neuronal cell types and which is required for inner ear and hypothalamus development. Binds to the 5'-CAAGTG-3' core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the HMX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565619.1">
        <property type="nucleotide sequence ID" value="XM_017710130.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IIE3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061864.1">
        <property type="protein sequence ID" value="ENSPNAP00000039108.1"/>
        <property type="gene ID" value="ENSPNAG00000030570.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108434765"/>
    <dbReference type="CTD" id="751654"/>
    <dbReference type="GeneTree" id="ENSGT00940000165038"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000053">
        <property type="entry name" value="H6 family homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051300">
        <property type="entry name" value="HMX_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110">
        <property type="entry name" value="HOMEOBOX PROTEIN HMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46110:SF5">
        <property type="entry name" value="SENSORY ORGAN HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="96"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="98"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="42"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="224" mass="24252" checksum="EE0F23D20D37CE66" modified="2024-11-27" version="1">MSKEDPQCRPASLKFTIDNILNLKSSGGAADSCGSKAPLCRGDFPPELANRDPDSRLQDAGSERVHADGEAPADEPLSSADGGCSQDGSAKKSKPAAKKKTRTIFSKRQIFQLESTFDMKRYLSSAERACLASSLQLTETQVKIWFQNRRNKLKRQLSTEMDGPNGDFGDGGKAVTFPGLYKENSLLGRCLLPMPLPVVYPGGSAPYLCLSNGAKYFGFFEGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKG6</accession>
    <name>A0AAR2IKG6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein VAC14 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">VAC14</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Scaffold protein component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Pentamerizes into a star-shaped structure and nucleates the assembly of the complex. The pentamer binds a single copy each of PIKFYVE and FIG4 and coordinates both PIKfyve kinase activity and FIG4 phosphatase activity, being required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms pentamers. Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14. VAC14 nucleates the assembly of the complex and serves as a scaffold by pentamerizing into a star-shaped structure, which can bind a single copy each of PIKFYVE and FIG4 and coordinates their activities. Interacts with NOS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VAC14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017579852.1">
        <property type="nucleotide sequence ID" value="XM_017724363.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKG6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044551.1">
        <property type="protein sequence ID" value="ENSPNAP00000038226.1"/>
        <property type="gene ID" value="ENSPNAG00000012941.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443607"/>
    <dbReference type="CTD" id="55697"/>
    <dbReference type="GeneTree" id="ENSGT00390000008385"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001093">
        <property type="entry name" value="Vac14, PIKFYVE complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026825">
        <property type="entry name" value="Vac14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021841">
        <property type="entry name" value="VAC14_Fig4p-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023:SF0">
        <property type="entry name" value="PROTEIN VAC14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16023">
        <property type="entry name" value="TAX1 BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11916">
        <property type="entry name" value="Vac14_Fig4_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Vacuolar protein 14 C-terminal Fig4-binding" evidence="13">
        <location>
            <begin position="543"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="340"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="782" mass="88479" checksum="E61549878EA77867" modified="2024-11-27" version="1">MNTEKDFSPLTPNIVRALNDKLYEKRKVAALEIEKLVREFVAQNNSTQIRHVIQILASEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDSDSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESNKFDLVAFVPLLRERIYSNNQYARQFIISWILVLESVPDINLLEYLPEILDGLFQILGDSSKEIRRMCEVVLGEFLKEIKKTPSSVKFAEMANILVIHCHLSDESKLTNDLIQLTAMTWMREFIQLAGRVVLPYSPGILTAVLPCLSYDDRKKITKEAASACNHSLMKLVTPEDDVEEEEESQSTTSPQSKDTSSKKEGDLNDSLNASQDSGGLINISFFTPPSERTQVTLDLDGIVQVLDRHLHDSSTGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPMLLKTLSDESDEVILKDLEVLAEIASSPAGQTESSGSCEGADKIELHVPDESKVGQQQLGVGSKGADSSPSTPSMNSYFYKFMINLLKRFSLERKLLESRGAFIIRQLCLLLHAENIFHSMADILLKEEDLKFASTMVQTLNTILLTSAELFQLRNQLKDLRTPESCTLFCCLYRSWCHNPVATVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLIEVDKLVQLIESPIFTYLRLQLLDVENNPYLIKALYGLLMLLPQSQAFQLLSHRLRCVPNPELMRTVEVPKPASAKHATKPQVDYQELLQHFDRVQSKHLEVRHQRASHAEHPDRKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKV9</accession>
    <name>A0AAR2IKV9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ADAM metallopeptidase domain 8a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551972.1">
        <property type="nucleotide sequence ID" value="XM_017696483.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKV9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046596.1">
        <property type="protein sequence ID" value="ENSPNAP00000040355.1"/>
        <property type="gene ID" value="ENSPNAG00000014023.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426763"/>
    <dbReference type="CTD" id="368917"/>
    <dbReference type="GeneTree" id="ENSGT00940000158585"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002693">
        <property type="term" value="P:positive regulation of cellular extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051044">
        <property type="term" value="P:positive regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022407">
        <property type="term" value="P:regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018358">
        <property type="entry name" value="Disintegrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF20">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043501725" description="ADAM metallopeptidase domain 8a" evidence="12">
        <location>
            <begin position="23"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="664"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="15">
        <location>
            <begin position="201"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="14">
        <location>
            <begin position="406"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="620"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="700"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="747"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="700"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="752"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="795"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="624"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="642"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="856" mass="94415" checksum="4A823361B2AD6AA4" modified="2024-11-27" version="1">MRCTGLLFSLLFHVSVWELCAAAHPRLSHVMHYDVVQLQPLKGRMKRSVSSQQKYPDTAEYALAVDGKNFTIYLERNRELLGKQYTLSYYTEDGMKVITPPSTTDHCYYQGHIQNMKDSSVSVGLCSGMRGFVRAENQVYLIEPLKKSLAGDHAVYKQEHLRTKRAAVQYINDTVYDLGPRFSGLYNSRNMNNKAPIGAQRFIEMVLVVDYKEYKKFGSLKDVQKRMLEVANHVDKLYRHLNIRVMLVGLEVWSKGDQIEVSSVPNLMLDRFLKWRQTTLLTRKKHDSAQFVTATDFDGSTVGLAPLNAMCSSRSGAVNEDHSINPLGVASTIAHEMGHNLGMSHDEPHCGCSSRSSQGCIMGESIGLVYPEAFSTCSKVALEMFLQNYDSSCLLDVPKEEDVYGGPVCGNAFVEKGEECDCGTVEECKNPCCNATTCRLTEGSKCAHGECCEKCQLKGAGGVCRHSAHDCDLEEYCTGKSAQCPKDDYKMNGLPCNYGQGYCYNGECPTLKEHCKKLWGPGADVDIDCFRHNTFDKSCARSGSGNCDQQHIKCGKIYCSGGNDFPITREKYLSTFGAKTCYSALEIFDKDSVGMVPTGTKCGTNKVCYNNLCQDLSVYKTKNCSAKCNNHGVCNHENQCHCDAGWAPPHCDVKLFNVANRKDAVIIGVTTSVVILLLITLVIGASVCCKKQKTPFNKQRSFKNTHTYSGQSNPVFQPSSAKNSPRCGPPRISQPTFMESTATQACKSLFSPTPPSRPAPQPPKLAEPRMEQTAKPSIPAPVVPSNTFVPQTKPLLPPSKPLPPSRPLPPLVSKPVYKPKSPPMPPVKPSGTQPAWNAPQVMAPPKVALKPPTKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IMA5</accession>
    <name>A0AAR2IMA5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GIPC2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GIPC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558860.1">
        <property type="nucleotide sequence ID" value="XM_017703371.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IMA5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047224.1">
        <property type="protein sequence ID" value="ENSPNAP00000039162.1"/>
        <property type="gene ID" value="ENSPNAG00000032061.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430720"/>
    <dbReference type="CTD" id="54810"/>
    <dbReference type="GeneTree" id="ENSGT00390000003420"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd21180">
        <property type="entry name" value="GH2_GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23078">
        <property type="entry name" value="PDZ_GIPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000097">
        <property type="entry name" value="PDZ domain-containing protein GIPC1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055349">
        <property type="entry name" value="GH2_GIPC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056814">
        <property type="entry name" value="GIPC1-3_GH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017379">
        <property type="entry name" value="GIPC1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12259:SF3">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN GIPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12259">
        <property type="entry name" value="RGS-GAIP INTERACTING PROTEIN GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25083">
        <property type="entry name" value="GIPC1_GH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25082">
        <property type="entry name" value="GIPC1_GH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038083">
        <property type="entry name" value="UCP038083_GIPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="123"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="323" mass="35386" checksum="AE6E14B1A826E14B" modified="2024-11-27" version="1">MPLGLRRKKNKSRESSHLVENEEVGGVGKTELNGDGVAPPPAALRPRLVFHTQLAHGSATGRIEGFSNVKELYGKIAEAFNIQPTEILFCTLNTHKIDMEKLLGGQIGLEDFIFAHIKGIKKEVEVFKAEDALGLTITDNGAGYAFIKRIKEGSVVDGVKVICVGDHIECINGKNIVGTRHYEVARMLKDLPKDQSFTLKLVEPMKAFDMLEPRSKGGKPGGENKIGTGRGTLRLRSKGPATVEEVPTEFEEKAVKKVDDLLESYMGIRDTELAATMVEVGRDKVNPDEFAMALDEALGDFAFPDEFVFDVWGAIGDAKQGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2INB3</accession>
    <name>A0AAR2INB3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SNW domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SNW1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Identified in the spliceosome C complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SNW family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2INB3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061505.1">
        <property type="protein sequence ID" value="ENSPNAP00000039484.1"/>
        <property type="gene ID" value="ENSPNAG00000013373.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010423"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="4">
        <location>
            <begin position="178"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="23"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="333"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="473"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="478"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="539" mass="61495" checksum="7EF2AB0569B6497C" modified="2024-11-27" version="1">VLYFLFYSFLPAPTQLSQDQLEAEERTRAQTSQSTALVSTRREPPPYGSRKGWVPRSLEDFGDGGAFPEIHVAQYPLDMGRKKKTSNALAVQVDAEGKIKYDAIARQGQGKDKVIFSKYTDLLPKEVLNDDAPELQRPDEEAVRELTEKTRAALEKQVSQKIAAAMPVRAADKQAPAQYIRYTPSQQGVAFNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKAREAVEMRAQVEKKMAQKEKEKKEERLRELAKMARDRRAGIKGHGEKGGEDGEVRERDEIRHDRRKERQHDRNISRAAPDKRSKLQRDQDRDISELIALGQPNPRVSSEAQYDQRLFNQSRGMDSGFAGGEDEIYNVYDQPFKSGRDMAQNMYRPSKNADKDMYGDDLDTLMQSSRFVPDREFSGTDHGPRRDGPVQFEEDPFGLDKFLEEAKQHGGSKRPSTSGRSKDYDHEKKRRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IQN7</accession>
    <name>A0AAR2IQN7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytokine-like nuclear factor N-PAC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glyoxylate reductase 1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nuclear protein NP60</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Putative oxidoreductase GLYR1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GLYR1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HIBADH-related family. NP60 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037400781.1">
        <property type="nucleotide sequence ID" value="XM_037544884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IQN7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072905.1">
        <property type="protein sequence ID" value="ENSPNAP00000042018.1"/>
        <property type="gene ID" value="ENSPNAG00000012949.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438010"/>
    <dbReference type="GeneTree" id="ENSGT00940000156435"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05836">
        <property type="entry name" value="PWWP_GLYR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000058">
        <property type="entry name" value="Putative oxidoreductase GLYR1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000011">
        <property type="entry name" value="putative oxidoreductase GLYR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000027">
        <property type="entry name" value="putative oxidoreductase GLYR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035501">
        <property type="entry name" value="GLYR1_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051265">
        <property type="entry name" value="HIBADH-related_NP60_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580:SF2">
        <property type="entry name" value="CYTOKINE-LIKE NUCLEAR FACTOR N-PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43580">
        <property type="entry name" value="OXIDOREDUCTASE GLYR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="11">
        <location>
            <begin position="8"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="509" mass="55631" checksum="781203CACB595466" modified="2024-11-27" version="1">MATVHLRIGDLVWGKLGRYPPWPGKVVSPPKDLKKPRGKKCFFVKFFGTEDHAWIKVEQLKPYHPHKEEMIKINKGKRFQQAVDAVEEYLKKAKGKEQAHASDDKNKTGKGQKTTAKPVKIIEEDDEDAFKGGSSDKDQTDSDPEPSSVQRVVAGTVAGFKWESSPVKDDPHFHHFLLSQSEKPASSMEPISKRLKIIEEDTGSTSIQAADSTAVNGSITPTDKRIGFLGLGLMGSGIVSNLLKMGHVVTVWNRTAEKCDLFIQEGARLGRTPAEVVSMCDITFSCVSDPKAARDLVLGPSGVLQGIRPGKCYVEMSTVDPETITELSQVITSRGGRFLEAPVSGSQQLSNDGMLVIVAAGDRSVYEDCSSCFQAMGKTSFFLGEAGNAARMMLILNMVQGSFMATIAEGLTLAQATGQSQQTFLDILCQGQMASTFVDQKCQNILQGNFKPDYYLKHIQKDLRLAISMGDSANHPTPMAAAANEVYKRAKALDQSDNDMSAVYRAYIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IWS3</accession>
    <name>A0AAR2IWS3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LRRC28</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017555004.1">
        <property type="nucleotide sequence ID" value="XM_017699515.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IWS3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075661.1">
        <property type="protein sequence ID" value="ENSPNAP00000042504.1"/>
        <property type="gene ID" value="ENSPNAG00000014781.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428481"/>
    <dbReference type="CTD" id="123355"/>
    <dbReference type="GeneTree" id="ENSGT00940000157771"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF12">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="104"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="366" mass="41132" checksum="D57E46A0A4BA805C" modified="2024-11-27" version="1">MASELHEAIFTAKQERHKNLFLNYRNLNIFPVELLKDEGLQFLERLYMKRNSLTTLPDNLAQKLPNLIELYLHSNNIAIIPQAIGNLAKLQSLDLSDNALQVICPEIGRLRSLRHLRLANNQLKFLPQELGDLKELETLDVSMNLLKTLPERLAQCLSLQCLTADRNRLHCVPRQLCLLPDLNELSMAANCLTSLPLDLGRSMELQFVFVDNNVHLKGLPSYLYNKVIGCSGCGLSLQVSDVKQLSLALGELSVPLPSEVKSIGSEADRVLTLEEMAMRTLHVAYSRPRKDAKFLSPFLLPNSLLDLLQCPLGHCHRCSQPMFTIVYPKLFPLRETALAGVHGRTTVSFVAYCCSSQCLQMFDLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IYE8</accession>
    <name>A0AAR2IYE8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IYE8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075855.1">
        <property type="protein sequence ID" value="ENSPNAP00000043077.1"/>
        <property type="gene ID" value="ENSPNAG00000018851.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183165"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="56"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="255"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="456" mass="50953" checksum="6DF0456B1D223665" modified="2024-11-27" version="1">MTQEDLCLRECISVHVGQAGVQMGNTCWELYCLEHGIQPDGQMPSQKPVGGHDDSFTTFFSETGAGKYVPRAIFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIIDSVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDFGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNSCFEPSNQMVKCDPRHGKYMACCLLYRGDVVPKDVNVAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSFEEDEEGEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0P2</accession>
    <name>A0AAR2J0P2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Adaptin ear-binding coat-associated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">NECAP endocytosis-associated protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NECAP1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NECAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0P2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060014.1">
        <property type="protein sequence ID" value="ENSPNAP00000045525.1"/>
        <property type="gene ID" value="ENSPNAG00000032033.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169205"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13228">
        <property type="entry name" value="PHear_NECAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000064">
        <property type="entry name" value="Adaptin ear-binding coat-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012466">
        <property type="entry name" value="NECAP_PHear"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847:SF15">
        <property type="entry name" value="ADAPTIN EAR-BINDING COAT-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847">
        <property type="entry name" value="ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07933">
        <property type="entry name" value="DUF1681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NECAP PHear" evidence="16">
        <location>
            <begin position="10"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="163"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042069"/>
        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="268" mass="29039" checksum="635DE7F2EBEA42B0" modified="2024-11-27" version="1">MTKMAAEAEYESVLCVKPDVSVYRIPPRASNRSVRAADWKLDTPDWTGRMRVTARGKVAYIKLEDKISGELFAQAPVEQYPGVAVETVSDSSRYFILRIQDDSGRSAFIGIGFGDRGDAFDFNVALQDHFKWVKQDNEFSKQALAPDSGPKLDLGFKEGQTITLNIGQSKRKEKSRPQSSGGFGLLPPPPGGKIAPPPTATSSSHSTEPLTGSSDTGCLLDLDSNNSDSQVQPTAPATNADLWGDFTSDSLSSQPAKQESSSTDWVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
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                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J207</accession>
    <name>A0AAR2J207_PYGNA</name>
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        <recommendedName>
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        </recommendedName>
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        <name type="scientific">Pygocentrus nattereri</name>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
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                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Nucleus</location>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1243" mass="139362" checksum="45BF3628FB078CE1" modified="2024-11-27" version="1">MSNNYYKSSPDDYRSRQDMYPDSYQRDDVYRQRTRSASQETMHSAASSSRPADSSALVPGKALSFLQSCGLDASDLSLLAELPEHLITVETLPKLLLQLKESKLSSSSSSRSSTAQPCAKTSSHAYESSTHVQAVGYSLDRSVRPSYPLPPDQVQSWQDRWGNRRKTSSSSSGTDSKTSTIVDYNHSRDGLYYNEQPYTSAASNTSSTISHSYTDYSKGPTQNSEPSHVISASTVFKVPTRKEASDFHGILPPVFPYACVLCNITVLTEKEWSVHIRGAQHAHNQLELVKKYPEWDQNVESAKRNARQATQQPRSTKAKDPYQSVPSRKEVSDIQGRIPPVFPIPSEKKDLSGDVRSTRHDNSQLVEKYPGWDRNIDSTNRNESQVATQAKLFENRERHYKTPTRKEASDFCGKVPPVFPYACVLCDITVLSEKDWSVHTGGVQHANGQLRLVERYPQWDQKIRSARRNVAQVISQAASTETKGGSQKENKKKNISDKEKRSNTKTPPKDHGRVVCVTFAASSVDEEYLKKLLGQFGAVVKVMMFPALALVEMGSKDQSGDIVKYFTDNPLAVEGKRIEFSISTTFSFLQSSRVVSFSPLPAGDGTTSELMAIAKRFGSVKTSLFLPSRGYVEMTDPADAKKLVEHFITKPLKLKGKAVQVTFSTEYDTLIDLTVDEKAQSLSSYSQGRSSPRRRSSQRDSPSPKRRRRSSERRRSSERRRSSERRHSPERRRSSERRHSPERRRSPEGRHSSERRHSSEGRHSPERRHSSERNCSSRSSRLNEQNDRQHSRDKSKTLSKASLVSSHCSNNDETTKPQSEDTEVKISVNSDIVASMDSDSDLEGLAVIADDGEELQHDDDEPIDHHKIDQEQKTCEEVAQQDDQTSSISAAFPKQDASSGREEVKHLTRQSQENSPSGSVGIPSVHSDVKVETLQDASKTSDSLKKNQKDEVLDIEEDFDFPESLENCITLDELVEETSSDYQESDKQNPSTPTAEEILDEEPTTINPPPLSSYMDSEENKKQSESPKEENTDSTAAETKAPEHESLNTDDQCLEQQDREKDTSNIKEEFGKVLDVRNLPKAEDYTDSDFVNIAKRYGEVKHYLLIRDCRKGFVEMVHASDAERIAADSKNQDVALRGNALKIKVSVKYSSLPTAGFNPDSDDEENTKRANSEDSTDDNQSVAGAESEKPNEPVGTEFVRPVVGYFCNLCNVIYASEEEAKDEHCRSPSHHEKLKEHKKNASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J4Z3</accession>
    <name>A0AAR2J4Z3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017548557.1">
        <property type="nucleotide sequence ID" value="XM_017693068.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J4Z3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050681.1">
        <property type="protein sequence ID" value="ENSPNAP00000045417.1"/>
        <property type="gene ID" value="ENSPNAG00000030300.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108424812"/>
    <dbReference type="CTD" id="799361"/>
    <dbReference type="GeneTree" id="ENSGT00940000158476"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18627">
        <property type="entry name" value="CD_polycomb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000006">
        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033773">
        <property type="entry name" value="CBX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF1">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17218">
        <property type="entry name" value="CBX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="7">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="373" mass="42997" checksum="FCDE2593FBC99782" modified="2024-11-27" version="1">MELSAVGERVFAAESIIKRRIRRGRMEYLVKWKGWSPKYSTWEPEENILDSRLFAAFEERERERELFGPKKRGPKLKTFLMKAQAKAKARSYEFRGESSRSIRITYPSTDALAAPRAREGLRAVVPTIFPPGTVNRGESIRLPPPEPPRELSPPRPTSDGFIYTPKKRGRKPKLRFSDGYSSGLLHPDKAKMSTEESMSGIPSKMARLELGEDDEDSEDRCSDLRAMKMSHRHQASPVYPYKPHRMIPSMDWSLHSSRMAANFQDCRTRDHLSHLHRKHLKHLKHLSHHRAFETVDQAARLQQQKPTLIAKIPVARILGEPEEEEEEDEEDEDTWSPCFSSVEKVIVTDVTTNFLTVTIKESSTDEGFFKDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J5T3</accession>
    <name>A0AAR2J5T3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">BRISC and BRCA1-A complex member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">BABAM2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the ARISC complex. Component of the BRCA1-A complex. Component of the BRISC complex. Binds polyubiquitin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
        <text evidence="15">Localizes at sites of DNA damage at double-strand breaks (DSBs).</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the BABAM2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J5T3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060991.1">
        <property type="protein sequence ID" value="ENSPNAP00000045604.1"/>
        <property type="gene ID" value="ENSPNAG00000011476.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004208"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070552">
        <property type="term" value="C:BRISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23664">
        <property type="entry name" value="BRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010358">
        <property type="entry name" value="BRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15189">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15189:SF7">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06113">
        <property type="entry name" value="BRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="13 15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 15" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 15" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="9 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043322145" description="BRISC and BRCA1-A complex member 2" evidence="16">
        <location>
            <begin position="19"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="401" mass="45428" checksum="04970172AA3FCE43" modified="2024-11-27" version="1">MHCCTSLMFLCCILTMNSLSPEVALSRISPELRPLLCSVVRNGRVGLDSTNCLRITDLKTGCTSLTPGPCCDRFKLHIPYAGETLKWDIIFNASDPELPPDFIFGEDAEFLPEPSELPHLVAWDAADPECLLQLVKELLQQYHQYQCQRLRDSSRLLFEYDSLLEDPNYGRSMEIYAGRKNSWTGEFSARFLLKLPVDFSNIPIYLLKDTALDPGEDVALLSVSFEDAEATQVFPKLFLSPSIEHALGGPSALHIPAFPSGGCLIDYVPQVCQLLTNKVQYVIQGYHKRREYIAAFLSHFGMGVVEYDAVGFTKLTLLLMWKDFCFLVHVDLPLYFPRDQPTLTFQSIYHFTSSGQLYSQVQKSYPYSPRWDGNEMAKRAKAYFKSFIPQFQEGAFANGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J823</accession>
    <name>A0AAR2J823_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycolipid transfer protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GLTP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553142.1">
        <property type="nucleotide sequence ID" value="XM_017697653.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J823"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046309.1">
        <property type="protein sequence ID" value="ENSPNAP00000046467.1"/>
        <property type="gene ID" value="ENSPNAG00000035997.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108427458"/>
    <dbReference type="CTD" id="565337"/>
    <dbReference type="GeneTree" id="ENSGT00940000165048"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032691">
        <property type="term" value="P:negative regulation of interleukin-1 beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000002">
        <property type="entry name" value="Ceramide-1-phosphate transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF93">
        <property type="entry name" value="CERAMIDE-1-PHOSPHATE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Glycolipid transfer protein" evidence="3">
        <location>
            <begin position="87"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="294" mass="32935" checksum="FCE52BD62554A9EF" modified="2024-11-27" version="1">MGVKGRAAFAIVFIIVFLCSLWLYGGLEPWGYCLKVYIQDEQSLSLYGNNVSEDDMVQLGFCPGQRFQVGVLLMHLQAAPLSTDDVLLEPYLASWDELIKFMDSLGPLVGFISQEIEAKTSIIRDLAQKEATAEEQVSGTSILKDEPTHPSAGVMGRAYTSVRSMIKAELAQGLVDFTQPQASGCRTLLRLHRALLWLQLFLHSLAEDGRAQSPSELCRDAYRVTLAQHHTWWVRKAAELAFVAMPDRAFFYRLVCVDTQEEAAVVLNHVVRAIQKVYNQTQMALEEHGMLELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J9X1</accession>
    <name>A0AAR2J9X1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor SYF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SYF2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing as component of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">May be part of a spliceosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SYF2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J9X1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063990.1">
        <property type="protein sequence ID" value="ENSPNAP00000047024.1"/>
        <property type="gene ID" value="ENSPNAG00000015570.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017845"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264">
        <property type="entry name" value="GCIP-INTERACTING PROTEIN P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="241" mass="28699" checksum="4286E18FCDE9F37C" modified="2024-11-27" version="1">MSAALKTKWTASDTTDDEPAETTASQKREERLRKFRELHFKRNEARKLNHQEVVEEDKRLKLPSNWEAKKARLEYELMVDAKKKECAERGEDYERVKLLEISAEDAERWEKKKKKKNPDPGFSGYAEAQLRQYQRLTKQIKPDMESYERQREEYGEDFHPTSNSLIHGTHVPSKEAINRMQEDVEKQIEKRSKYSRRRAYNDDADIDYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JD96</accession>
    <name>A0AAR2JD96_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RASL11B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017543058.1">
        <property type="nucleotide sequence ID" value="XM_017687569.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JD96"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075752.1">
        <property type="protein sequence ID" value="ENSPNAP00000048204.1"/>
        <property type="gene ID" value="ENSPNAG00000031984.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108414670"/>
    <dbReference type="CTD" id="65997"/>
    <dbReference type="GeneTree" id="ENSGT00940000158643"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04146">
        <property type="entry name" value="RERG_RasL11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="200"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="247" mass="27202" checksum="5F0F99CFC116B0F2" modified="2024-11-27" version="1">MRLVQNMSTIAEYPGTDCLSNRVIKIAVVGGSGVGKTALVVRFLTKRFIGDYERNAGSLYSRDVQLDGEQVAIQVQDTPGVQVNANGLSCTDHVTRSIQWADAVVMVYSVTDHRSFDLIGQLHQLVSHAHAERSTLPPIILLANKADLLHVRRVDAQQGPLLASALSCFFYEVSASEDYSQVHSAFHRLCRELAKQQPLPSQPSVTAAGSEKRRSPLIPRPKSPNMQDLKRRFKQALSAKVRTVTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JFE8</accession>
    <name>A0AAR2JFE8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Early endosome membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017538681.1">
        <property type="nucleotide sequence ID" value="XM_017683192.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JFE8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073823.1">
        <property type="protein sequence ID" value="ENSPNAP00000048571.1"/>
        <property type="gene ID" value="ENSPNAG00000037789.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108411561"/>
    <dbReference type="CTD" id="337737"/>
    <dbReference type="GeneTree" id="ENSGT00940000154337"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000180">
        <property type="entry name" value="Member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="216" mass="23488" checksum="D6F731477DB7F430" modified="2024-11-27" version="1">MANRGGATRPNGPNAGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQDAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKRLPKSEPQAAGTNSGRSRGVDLTETAPPAKAPCCSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JFQ6</accession>
    <name>A0AAR2JFQ6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JFQ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047377.1">
        <property type="protein sequence ID" value="ENSPNAP00000049157.1"/>
        <property type="gene ID" value="ENSPNAG00000033551.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000279404"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="181" mass="18281" checksum="41E80674C9830CC9" modified="2024-11-27" version="1">YSSYANSMPIPNPTSTSIPNPTSTSIPNPTSTPISNPTSTSIPNPTSTSIPNPTSTPIPNPTSTSIPYPTSTSIPNPTIPNPTSTSIPNPTSTSIPNPTSTSIPNPTSTSIPNPTSTSISNSTSTPVPNSTSTPIPTRSSIPNPTGTLISNPTSTSISNSTSTSIPNSTCTPIPNPTSTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JPM8</accession>
    <name>A0AAR2JPM8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JPM8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050463.1">
        <property type="protein sequence ID" value="ENSPNAP00000053965.1"/>
        <property type="gene ID" value="ENSPNAG00000031890.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164735"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="29606" checksum="A09667DA8C36B1B7" modified="2024-11-27" version="1">IIDNQILSNPDPHLLKKKTELQSKFDLLSTTQAEKRLLQSKGYLGIHITRKFSHLHDINLETLLQKTKLYFQKWKCLPLTLMGRVNVVKMTILPKFLFVFQCIPLFIPKSFFRSLNQLISSFLWDGKPPRISIRTLQKPHSQGGLALPNFLFYYWAANIQKLIFWLQTPRADWCLLEASSCNQSSLPALIHSSLPIALARKSTNPIVYSTLRIWSQFCSHIKDFNQGITKFKDLFDENTKIFLNFPDLQIPNSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JQZ3</accession>
    <name>A0AAR2JQZ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 10">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 10"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JQZ3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073204.1">
        <property type="protein sequence ID" value="ENSPNAP00000054438.1"/>
        <property type="gene ID" value="ENSPNAG00000007451.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000707"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF5">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="570" mass="64262" checksum="6A9847B9DBC305B4" modified="2024-11-27" version="1">TILYCFYEVILKDIICCLWFLPTMTSGTLEQWYKSNAGGLNMRQMFDADKERFSKFSLNLETDDGDILLDYSKNLINEEVLKMLFDMARSIGVETARDQMFAGEKINFTEGRAVLHIALRNRANTPIMVDGKDVMPEVNRVLKKMKDFCHRVRSGEWKGFSGKTITDVVNIGIGGSDLGPLMVTEALKPYSKGGPNVWFVSNIDGTHMAKTLAQLNAETTLFIIASKTFTTQETITNAESAKEWFLQTANDPSAVAKHFVALSTNAPKVKEFGIDTENMFEFWDWVGGRYSLWSAIGLSIALHIGFENFEQLLAGAHWMDKHFCSAPLEQNAPVLLALLGVWYVNFFQAETHALLPYDQYMHRFAAYFQQGDMESNGKYISKSGTRVNYHTGPIVWGEPGTNGQHAFYQLIHQGTRMIPADFLIPAQTQHPIRDNLHHKILMANFLAQTEALMKGKTPDEARKELEAAGKSGDALEKLLPHKVFQGNKPSNSIIFKKLTPFMLGALVAMYEHKIFVQGVVWNINSYDQWGVELGKQLAKKIEPELQDDSEIHSHDSSTNGLISFFKKNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K0M5</accession>
    <name>A0AAR2K0M5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">N-acetylglucosamine-1-phosphotransferase subunits alpha/beta</fullName>
            <ecNumber evidence="17">2.7.8.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">GlcNAc-1-phosphotransferase subunits alpha/beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Stealth protein GNPTAB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">GNPTAB</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(4)-[alpha-D-mannosyl-(1-&gt;2)-alpha-D-mannosyl-(glycan)]-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-[6-(N-acetyl-alpha-D-glucosaminyl-1-phospho)-alpha-D-mannosyl-(1-&gt;2)-alpha-D-mannosyl-(glycan)]-L-asparaginyl-[protein] + UMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13581"/>
            <dbReference type="Rhea" id="RHEA-COMP:14507"/>
            <dbReference type="Rhea" id="RHEA-COMP:14508"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:140357"/>
            <dbReference type="ChEBI" id="CHEBI:140369"/>
            <dbReference type="EC" id="2.7.8.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the stealth family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.17" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2K0M5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084660.1">
        <property type="protein sequence ID" value="ENSPNAP00000057863.1"/>
        <property type="gene ID" value="ENSPNAG00000018089.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006747"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003976">
        <property type="term" value="F:UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046835">
        <property type="term" value="P:carbohydrate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016256">
        <property type="term" value="P:N-glycan processing to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21600">
        <property type="entry name" value="RRM2_GNPTAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.320:FF:000002">
        <property type="entry name" value="N-acetylglucosamine-1-phosphotransferase subunits alpha/beta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041536">
        <property type="entry name" value="GNPTAB_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035993">
        <property type="entry name" value="Notch-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047141">
        <property type="entry name" value="Stealth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031358">
        <property type="entry name" value="Stealth_CR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021520">
        <property type="entry name" value="Stealth_CR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031357">
        <property type="entry name" value="Stealth_CR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031356">
        <property type="entry name" value="Stealth_CR4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24045">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24045:SF0">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-1-PHOSPHOTRANSFERASE SUBUNITS ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18440">
        <property type="entry name" value="GlcNAc-1_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00066">
        <property type="entry name" value="Notch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17101">
        <property type="entry name" value="Stealth_CR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11380">
        <property type="entry name" value="Stealth_CR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17102">
        <property type="entry name" value="Stealth_CR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17103">
        <property type="entry name" value="Stealth_CR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01137">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00004">
        <property type="entry name" value="NL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90193">
        <property type="entry name" value="Notch domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51912">
        <property type="entry name" value="DMAP1_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50258">
        <property type="entry name" value="LNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="LNR" evidence="25">
        <location>
            <begin position="444"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="DMAP1-binding" evidence="26">
        <location>
            <begin position="707"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="24">
        <location>
            <begin position="991"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="777"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="647"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="777"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00082117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1253" mass="141337" checksum="E028EEB0AEAA456F" modified="2024-11-27" version="1">MISSVLKVLQRQTYTCLSHRYGLYLCFGGIVLMIVSAFQFGEVVVEWSRDQYHVLFDSYRDNVAGKSFQTRLCLPMPIDVVYTWVNGTDTDLLKDLRAVRQQLEEDQRKLREKLGKNATDTTEAPKGRPECLLSHCIMAPALVLDPSLPANITVKELSSLSAALSSTKQLLQVAKPLHPSTSVSVLLFHSQSEGAVTHTHILGSWSRHVFRGYKYITIYYHKDAPSLVRIQTLAYLSGFPASFKETEQLRIKLPAVVTSKIKQLQLYSEASIALLHLNTAQDFTDLLQQAKKNLTLDGKELTVSPAYLFWDISAVSQSKQDEDVSASRFEDNEELRYSLRSVERHAPWVRHIFIVTNGQIPSWLNLDNPRVTVVTHQEIFQNQSHLPTFSSPAIETHIHRIPGLSQKFIYLNDDVMFGKDVWPDDFYSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKACNNSACDWDGGDCLGTAGGSRFGGGGAGGSVIGGAQPWQFAGGLGGLGGVSFCNQGCANSWLADKFCDQACNVLACGFDVGDCGQDNFGQLYRVVLQRNKTQYALPQGELRPYFIFSAFANRVSEAQVGDNPMVRHTSVANKWKTIHLLLLPGYNATQIHYNLTFQGTGDHEFMMSITVAVDTRELPKRNASDSMQDKGNEEHKPTVATPEPEVQFADIPVEKQAPKVRKKPSGEVEVIVEVPALNTSLLPEVVRHELQRLDEKLRVGDITLKGYNLTKATLLKPFQDRAALETQTDKRIDEGKADEGGIVVERVADASKSDGKQSQMEKKDEQKVSLIPVKIDNEPALADQSQLKSTEKSPASRLLETLVGSMSKGRGSEAQAHEQNPQAGDEKAELEPHVGRKLQLYVGPEWSFLPWEKSKYFQDLLEERERLERELEYKADGAATGRRLKDTFADSLRYVNKLLNGQFGFTSRKVPAHMPHMIDRLVMQELQDTFPGEFDTTSSHRVRHSEDMQFAFSYFYFLMSALQQLNVSQVFDEIDTDHSGVLSDREIRTLATRIHELPLSLQDLTGLEQMLINCSKTLPSNQTQLHMVNPTQETYYDPSMPPVTKGLVIHCKLIAEKIQKAFKDQNKYKFEIMGEEEIAFKMVRTNVSHVVGQLDDIRKNPRKFICLNDNIDHSHKDASTVKAVLRDFYESMFPLPSQFELPREYRNRFLHMGELQEWRIYRDKLKFWTHCVLVTLVVFTVISFFAEQVLSGAHLIVIWCSPHGIRYITILILQSPKNVFGTCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1E9</accession>
    <name>A0AAR2K1E9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1E9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073456.1">
        <property type="protein sequence ID" value="ENSPNAP00000056322.1"/>
        <property type="gene ID" value="ENSPNAG00000034822.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248873"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045314385" evidence="2">
        <location>
            <begin position="19"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="279" mass="29141" checksum="1A720530DBF64E65" modified="2025-10-08" version="2">MLWTVLSLFYCTVVCCTAVCCTAVCCTAVCCTAVCCTAVCCTAVYFCCTAVCCTAVYCTVVCCTAVYCTVVCCTAVYCTAVCCTAVCCTAVCCTAVYCTAVYCTAVCCTAVYCIAVYCTAVYCTAVCCTAVCCTAVYCTAVCCTAVCCTAVCCTVVCCTAVCCTVVYCTVVCCTAVFCCTVVCSTVVYCTAVYCTVVCCTVVYCTAFFCTAVYCTVVYCTVVCCTVVYCTAFFCTAVYCTVVYCTVVCCTVVYCTAFFCTAVSLLACVRVTAAVLSSHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K5Q8</accession>
    <name>A0AAR2K5Q8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxyhypusine synthase</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K5Q8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061320.1">
        <property type="protein sequence ID" value="ENSPNAP00000057792.1"/>
        <property type="gene ID" value="ENSPNAG00000008224.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008063"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="362" mass="40498" checksum="DF69181C80E11E53" modified="2025-10-08" version="2">MEYYYSVPPLAREAVLKESTALPEDVPQIRGYDFNQGVDHRALLHSYLTTGFQASSFALAVQEINKMIEKRLEPVGRDEEQQDQRDCPGCTIFLGYTSNLISSGVRETIRYLAQHKMVDVIVTTAGGVEEDLIKCLAPTYLGEFSLPGKELRQRGINRIGNLLVPNDNYCKFEDWLMPILDQMVLEQKTEGTHWSPSKMIHRLGKEINNPDSVYYWAYKNDIPVFSPALTDGSLGDMIYFHTYKNPGLVLDIVEDIRRLNSKAVFAKSTGMIILGGGLVKHHIANANLMRNGADFAVFVNTGQEFDGSDSGARPDEAVSWGKIRMDAKPVKVYADASIVFPLLVAETFALHASRLTVDKKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K637</accession>
    <name>A0AAR2K637_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lon protease homolog 2, peroxisomal</fullName>
            <ecNumber evidence="8">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 17" type="primary">LONP2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9 12 13">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2K637"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074492.1">
        <property type="protein sequence ID" value="ENSPNAP00000057571.1"/>
        <property type="gene ID" value="ENSPNAG00000015490.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063553"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000207">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000003">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.40:FF:000003">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000019">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000382">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03121">
        <property type="entry name" value="lonp2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027501">
        <property type="entry name" value="Lonp2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="2 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="16">
        <location>
            <begin position="13"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="15">
        <location>
            <begin position="651"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="598"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Microbody targeting signal" evidence="8">
        <location>
            <begin position="851"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 10 12">
        <location>
            <position position="743"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 10 12">
        <location>
            <position position="786"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="387"/>
            <end position="394"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="853" mass="93691" checksum="04829D91542C30E5" modified="2024-11-27" version="1">MSSSGGIQIPSRLPLLLTHEGVLLPGSTTRFSVDSARNMQLVKSRLLKGTSLKSTMIGVIPNTKDPEHDSDELPSLHRCHGCACCSVFCSIGTAGLAVQVVGSNWPKPHYTLLITGLCRFRVSHLLRERPFPVAMVEQLDRLEQYTEGGQLDGELGELSQRFYQAAVQLVGMLDMSVPVVAKLRRLLDSLPRETLPDVLASMIRTSNLEKLQVLDAVDLEERFKKTLPMLTRQIEGLKLLQKTRKLRPDDDKRVLAMRKGGVFPGRQFSLEEDAEDEDGDDMAMLERKVKEAGMPEPALRVCLKELKRLKKMPQSMPEYALTRNYLELMVELPWSKSTTDCLDIRAARVLLDNDHYALEKLKRRVLEYLAVRQLKSALKGPILCFVGPPGVGKTSVGRSIARTLGREFHRIALGGVCDQSDIRGHRRTYVGSMPGRIINGLKMVGVNNPVFLLDEVDKLGKSLQGDPAAALLEVLDPEQNHSFTDHYLNVAFDLSQVLFIATANTTATIPPALLDRMEVLQVPGYTQEEKVEIAHRHLIPHQLEQHGLTPQQLQIPQDTTQEIISKYTREAGVRSLERKIGAICRAVAVKVAEGHKLSRTDSSSEPGALFTKPAEDSDMTAPPEMPIVIDQVALKDILGPQIFEMEVSERLTLPGVAIGLAWTPLGGEIMFVEASRMEGEGQLTLTGQLGDVMKESAHLAITWLRSNAKTYQLTNIAGCADPLEGNDIHLHFPAGAVTKDGPSAGVTIVTCLASLFSGRLVRSDVAMTGEITLRGLVLPVGGIKDKVLAAHRAGLKRIILPKRNEKDLEEIPSHIRAELDFVTASTLDEVLNAAFDGGFPAATVTHSQIASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K6Z1</accession>
    <name>A0AAR2K6Z1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZCCHC24</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K6Z1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062413.1">
        <property type="protein sequence ID" value="ENSPNAP00000060073.1"/>
        <property type="gene ID" value="ENSPNAG00000036552.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286934"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF152">
        <property type="entry name" value="SI:DKEY-210J14.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="239" mass="26895" checksum="8E12773B1594C96C" modified="2024-11-27" version="1">LSSSFLVKFLMSFNILCKNELQWNKFVSDCNMLFICVVLGSAFPGTEQDGGHSSENFIPYNDPLNSTEQTQEGDQTFDPRPSTLLDGAASSNMNLDEFGGYYPSSVHLGIPKTGLEQKKIECVFCGKNFNYLSYLKVHLRTHSGEKPFVCSVCGRRFAQKTYLKIHMRTHSGERPYSCMECGKSFSQKSSLNIHLRSHTGEKPYSCVDCGRRYAYKHGFNTHHCTKVEKQGITKTGAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KB77</accession>
    <name>A0AAR2KB77_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ubiquitin-related modifier</fullName>
            <shortName evidence="7">SUMO</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SUMO2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex sae1-sae2 and linkage to the E2 enzyme ube2i.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KB77"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065047.1">
        <property type="protein sequence ID" value="ENSPNAP00000061573.1"/>
        <property type="gene ID" value="ENSPNAG00000016341.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182895"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd16115">
        <property type="entry name" value="Ubl_SUMO2_3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000022">
        <property type="entry name" value="Small ubiquitin-related modifier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="101" mass="11677" checksum="F84D07D994441D39" modified="2024-11-27" version="1">RHHPTPLSLEDKFSFITTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLTMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KC33</accession>
    <name>A0AAR2KC33_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017564086.2">
        <property type="nucleotide sequence ID" value="XM_017708597.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KC33"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069771.1">
        <property type="protein sequence ID" value="ENSPNAP00000059656.1"/>
        <property type="gene ID" value="ENSPNAG00000032747.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433797"/>
    <dbReference type="CTD" id="566196"/>
    <dbReference type="GeneTree" id="ENSGT00940000155128"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF7">
        <property type="entry name" value="FH2 DOMAIN CONTAINING 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="37"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="697"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="697"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="764"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="783" mass="87269" checksum="A5536CF70C69DE24" modified="2024-11-27" version="1">MADPPPPPPPPPPPPPPPLPPPPPPQPPALNSGGNLLSRGSRRSSRMRNFYWEAIPQDTVLGKHNIWTAEKKKEYELDTQRMEELFSRSDLTDSKGPASVRRSIKGTQSKAQKLQGPEVVSILNGKRNMNVGIFLKQFRRSVKEVIELIRLGKNEAFGSGKLEELLKLLPDSGEVKQLLAYKGDYAILSEADQFMVQLVKVPCYEERLKSLVLKEEFPHFMDEVNHCIAIMTAAGKELLDCLDLHSIIRLVLKTGNYMNADGYAGSAVGFRMMSLLKLADTKANRPGMNLMHYVVMQAQKSDAALLKFPEQLAHIGEASRIHKQDIESEFQRKVSEVQELKKSTDKQRDLAEQMNDFFKCAESRIAETKASFQTLNAVTESVAEYFCEEASDFRLDECCSIFHSFCERFKRALQENMDRDLAEAKRRQREKLQCAAKRRSTATCSMRDKDMEGVALESVLQRFLTNRGSHRQSRTLSPTGIRLSEITSQENCPAKNSKKPNWTLNLSQASVDKENVEDKASKKSESKISSPMWKKPASSTPAGNRALMTLAASEDEDGQTEEEAQKLRETSQKVLRYQSSRSSVSSGEYCSPASSPRRRVFQEDKEKTLANSNVEDVPKPPASPLVLTQNRLGSISRRHSISLPTRAPQGSDSEEDMFIPSSEVNGSPRGQIVSLGNIGKMRSVDGCLLSTKARLGSETTGNVSNNMSTNMPADAPKESSKDVISPAELIKVEAKEQTDESSSKSKVSQRQTKESSRFISFFRRLGEKGKTNNREAESSSVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KGA0</accession>
    <name>A0AAR2KGA0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KGA0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060866.1">
        <property type="protein sequence ID" value="ENSPNAP00000061479.1"/>
        <property type="gene ID" value="ENSPNAG00000036242.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000274684"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043568828" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="35"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="185" mass="21034" checksum="906410E4107DCF50" modified="2024-11-27" version="1">MIKEELFICLFIFYLAASLSSVLWQTPGTVWVPVGEAFTLQCIHEMKVQYCYSATSWHKVSKRTGRLNEIRPELNMKQTDTKVCTLTIPNATLQDSGMYHCTGLYNKIVLVGNGTRVIVTGELVSVFHFTHRVYSTLSIQDLKTLFSHQGRKWKAVTYRHASLPNANRMPLLSEENSISVISFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KIJ0</accession>
    <name>A0AAR2KIJ0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Selenoprotein P N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KIJ0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069856.1">
        <property type="protein sequence ID" value="ENSPNAP00000064138.1"/>
        <property type="gene ID" value="ENSPNAG00000031728.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000049326"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001887">
        <property type="term" value="P:selenium compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007671">
        <property type="entry name" value="Selenoprotein-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037941">
        <property type="entry name" value="SeP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10105">
        <property type="entry name" value="SELENOPROTEIN P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10105:SF4">
        <property type="entry name" value="SELENOPROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04592">
        <property type="entry name" value="SelP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Selenoprotein P N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="171" mass="19575" checksum="FAA302A9BB498F76" modified="2024-11-27" version="1">MYWELKRRTAEGIPVYQQRPLQSDVWETLQGDKDDFLVYDRCGQLTFHIVLPYSFLHYPYVEAAIRATYHKDICNCTVDSNSTLSADLRNSSSVAKGENQTMTSSEPTRPLVDRADHHSHHHHDHPSQSHGHTSHSTNPLITKAGESHHSTHNTEYEGHITHKHEHHPADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KPX6</accession>
    <name>A0AAR2KPX6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating per1 and per2, which may lead to their degradation. Participates in wnt signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer. Interacts with per1 and per2. Component of the circadian core oscillator.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KPX6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082683.1">
        <property type="protein sequence ID" value="ENSPNAP00000064534.1"/>
        <property type="gene ID" value="ENSPNAG00000026439.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153536"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000194">
        <property type="entry name" value="Casein kinase I isoform delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="301"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="473" mass="54114" checksum="89E26F47308C54DE" modified="2024-11-27" version="1">MDLRVGSKYRLGRKIGSGSFGDIYLGSNIATGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAVDEGDRERRGEEREGGGVRGSGVRGHAPPGPNRVRNGPEQPSSNPASRIQQSGNTSPRAISRGERDRKVSMRLHRGANVSSSQVHTRTLFHPHKYTHFQESLIHTATHTHKPCRECSYPPHPSLLFCSFIAYSLCRLACHMSTLESELSNHTHNDRPVFFDPLFDSPTHQCTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KQU1</accession>
    <name>A0AAR2KQU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetratricopeptide repeat protein 7A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TTC7A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane. The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis. In the complex, plays a central role in bridging PI4KA to EFR3B and HYCC1, via direct interactions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017546092.1">
        <property type="nucleotide sequence ID" value="XM_017690603.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KQU1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084014.1">
        <property type="protein sequence ID" value="ENSPNAP00000064859.1"/>
        <property type="gene ID" value="ENSPNAG00000013367.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108422500"/>
    <dbReference type="CTD" id="57217"/>
    <dbReference type="GeneTree" id="ENSGT00940000158638"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000066">
        <property type="entry name" value="Tetratricopeptide repeat domain 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000105">
        <property type="entry name" value="Tetratricopeptide repeat domain 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045819">
        <property type="entry name" value="TTC7_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF475">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19440">
        <property type="entry name" value="TTC7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide repeat protein 7 N-terminal" evidence="13">
        <location>
            <begin position="38"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="11">
        <location>
            <begin position="440"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="886" mass="98085" checksum="D4A3F7A79E0252A2" modified="2024-11-27" version="1">MANLCKENETAKSIFRRLECAVTPLVSGDYGHRDIAGMSSRISFSQYRLEAEIERCRAECQWDKIPPLLEQLAAARLHEQDDYGTLLLAEALLEECLLENVTLLKTSTPLSDQTQPKLAKAKAHLNSLLSRGRLEPHYLNEALLLLAKVHYVQGRYRDAQGMCARAGIEEFTRDEQPIYHLRLLAEAFVIKGLSLDHQTVSAASRVRLSEREEECLSCYLRACDTALLYLQELDKTVSTPSAKTAKSSISSPPVEVDPGYFLQAALQSAYLCLLHKGHLAQGTHQLRRVMRVMESRGSRSFRKGVSRKLAEVLLSSVSEDSYWGPLSTPPPTWLHMEGATHPKETIYPSSRPPQRYSTEGAFCPQDVVEEAVLLLLITESMASGEAVISRTPEHQEAREASIQEASSVYDMLSIGMARRGQYAMLSQCLERAMKFSFNEFHLWYQLGLSLMASGKAEGAVSVFKECARMKPQDPTPPLLAAKVCINQLHWIEEAEALAQSVVALGEGAEESLARAHLAIGLCCSLRASDATLKGDRDEFNRRALQALQTAHEVDPQDPQIALYLSLQLALVRQVSAAMEPLQLALRLRSEDLHSLHLLALLLSAQKHYQHALDTLTVALNQYPENFNLLFTKVKLEEALFGPAVALQTCEDMLQLWQSRYDFARPSEVDDSSSIPPPEPSPISRKSSGLHLTLPDFQETETGSQSAPSLAVSRLEQAMSEVSAISSTHRQGPAYIWTTLERIWLQAGELFMADGRVKEAQFCVQEAAALFPTSHSVLLLKGQLAELRGNDAEAKSLYDEALAINPTGQHILIHMGKLLVRTGRVGLGEKVLRDAVQAHSVSHEAWSGLGEALQSRASTQAPDCFLTALELEASCPIRPFTVIPREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KS07</accession>
    <name>A0AAR2KS07_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KS07"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059165.1">
        <property type="protein sequence ID" value="ENSPNAP00000067055.1"/>
        <property type="gene ID" value="ENSPNAG00000037502.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234621"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF41">
        <property type="entry name" value="P2Y PURINOCEPTOR 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="59"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="415" mass="47636" checksum="A9A01D3C2DB05076" modified="2024-11-27" version="1">MKSSASFRAMAGSSVDSNFSSNHSASTLTELFNCSNDESYKYIVLPLCYCITFLLSVILNSTVLLRSYRGAHRWNASLIYMVNLASTDLMYSLSLPLLVASYVMRDNWVFGDFMCRLVRFLFYFNLYCSIFFLTCISVHRYMGICHPIRTIALESKRAVKSVCAMVWVMVFLLTCPIFRFAQTGKVMRVVENGDEEDLGSRQRLSRVGLEMYRNCFDVATDNEFSEYVPYGIILHLLGFFVPFIIIAWCYSQVVRTIFQTLHTHPYVQEFGDTERGPSEDRYRTSISIAGSQYSPHISRRRKSIKTIITITFLFALCFLPFHITRTLFLILKQRNAECNTMHAISVCYKITRPLASCNSWLNALLYFLTGDKTIPCCGCTEGNAHICLVWPWRIFRGLKKREGGEQQGKTCKPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LC66</accession>
    <name>A0AAR2LC66_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Meteorin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the meteorin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LC66"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075957.1">
        <property type="protein sequence ID" value="ENSPNAP00000071971.1"/>
        <property type="gene ID" value="ENSPNAG00000017173.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001390"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090336">
        <property type="term" value="P:positive regulation of brown fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051998">
        <property type="entry name" value="Meteorin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28593">
        <property type="entry name" value="METEORIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28593:SF1">
        <property type="entry name" value="METEORIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043389372" description="Meteorin-like protein" evidence="9">
        <location>
            <begin position="31"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="305"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="375" mass="41806" checksum="821530CBC0072F79" modified="2024-11-27" version="1">MKARMRMRRALCSFALALLLLAGCVWSGAAQVSTDQCSWRGSGLTHQSHVRDVEQVYLRCSEGSLEWLYPTGAVIVNLRPNTDPLLTPKHAQMVCLRPRPGSSGASVYLWRDGELTLLLSEEDQAAGRQRCFTLQQQRALFIEATAHTDISKRVTAFQYQLINGETGDQPYSPTAPCRPCTDEQILMAVCTSDFVVRGSIQVIEQADDDEEDQASLVVLSSRVYRQKKRVFVPAGGGTRASSRWTGHVKVPRKCGLRIEQGVEFLFTGSVMFGEAWLGCAPRYKDFLQVLSLYGKIHRSVHVPMSVTSGDGGKKKKAKEETLRGTKTQKENPSTSGRHRTANNISIKYHNAKKGKSRNVLYIVLYRTLKGFYTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LH89</accession>
    <name>A0AAR2LH89_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Vav guanine nucleotide exchange factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">VAV1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LH89"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075320.1">
        <property type="protein sequence ID" value="ENSPNAP00000075943.1"/>
        <property type="gene ID" value="ENSPNAG00000016844.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159125"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20867">
        <property type="entry name" value="C1_VAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01223">
        <property type="entry name" value="PH_Vav"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11979">
        <property type="entry name" value="SH3_VAV1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000009">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000015">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000019">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037832">
        <property type="entry name" value="PH_Vav"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035730">
        <property type="entry name" value="VAV1_SH3_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818:SF2">
        <property type="entry name" value="PROTO-ONCOGENE VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="16">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="15">
        <location>
            <begin position="195"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="403"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="517"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="606"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="683"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="796"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="859" mass="98290" checksum="AC80F6FE802EFC9F" modified="2024-11-27" version="1">MELWRQCASWLIQCRVLPENHRVTWDSAQVCDLAHALRDGVLLCHLLNNLLPHAVNLREINLRPQMSQFLCLKNIRTFLSACHDRFGIKKSELFEAFDLFDVRDFGKLSCLSLSLSVSLSLSLSLSLTLSLSLSLSVSASLPVFLSLSLSLSLSLSDTVDEDDDLYDCVEDEENEGDDIYEDLMRTEEPPETVDKRSCCLQEIQQTEEKYTQTLESILLHFMKPLQAFLKPNDIENIFINVEDLAGTHRCLLKEVQSSISTYGAENLYQVFIDYKEKLLLYGRYCSQVESATKHLDKIASMKEDVRMKLEECSMRANSGRFSLRDLLMVPMQRVLKYHLLLQELVKHTVNPTDKENLRTALDAMRDLAQCVNEVKRDNEIIRQITAFQLCIENMTQSLALFGRPKIDGELKICSPEKKSKQDRYAFLFDKALLVCKKRSGESMELKELIDLQHFQLRDEPSGDKDHKKWTHTFLLMDSYGQGGYDLFFKTRELKKKWLEQFEMALSNLCPDNSTANGHDFQMYCFDDTTSCKACQMLLRGIFFQGYRCSRCKMAAHKECLGRVPACGRIPSLGTASGPTARLLTSRLIPVAKPRRSAGAQETGLKIRLPKMEVCHDYYGMPPPPVAFGQPLHLTIGDVIELTRADVDLQWWEGRNLTVGEVGWFPCSKVQPFLPVSEPRVKSSFAGNMDRTEAKSLLMSRSDGTFLVRQKDGGEFAISIKFNMDIRHIKITLAEGLYRINDKKAFKGLIELVQYYQENSLKECFKDVDTRLQIPFKQPEQSGSSYTHQPTRSASERYFGTAKVRYDFSARDRTELSLREGDTVKIISKKAHNGWWKGEVYGRVGLFPANYVEEEHSDYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIH5</accession>
    <name>A0AAR2LIH5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SMARCA2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551000.1">
        <property type="nucleotide sequence ID" value="XM_017695511.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017551001.1">
        <property type="nucleotide sequence ID" value="XM_017695512.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000052323.1">
        <property type="protein sequence ID" value="ENSPNAP00000076358.1"/>
        <property type="gene ID" value="ENSPNAG00000014635.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426196"/>
    <dbReference type="CTD" id="6595"/>
    <dbReference type="GeneTree" id="ENSGT00940000154821"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05516">
        <property type="entry name" value="Bromo_SNF2L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000004">
        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.120:FF:000001">
        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000089">
        <property type="entry name" value="Putative global transcription activator SNF2L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003020">
        <property type="entry name" value="SNF2-related domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006576">
        <property type="entry name" value="BRK_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037259">
        <property type="entry name" value="BRK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07533">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08880">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00592">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00951">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160481">
        <property type="entry name" value="BRK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="10 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="QLQ" evidence="21">
        <location>
            <begin position="175"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="HSA" evidence="20">
        <location>
            <begin position="449"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="748"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1066"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="1413"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="105"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="212"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="565"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="637"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1356"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1500"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="662"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1365"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1504"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1541"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1557"/>
            <end position="1567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1590" mass="180484" checksum="7D20129252BDA3B7" modified="2024-11-27" version="1">MSTPTDPSVGMSHPGPSPGAGLSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVPHSMQSQGTADYSQDGMHSMHKPMEGMHEKGMGDDMHYGQMKGVGMRSLHSGMGPPQSPMDQHSQGYLSPHPSPMGPIPEHVSSPMSGGGPTPPQMPQAQSGPMMQMDPQGMGQQGRGHSAFSPVQLQQLRAQILAYKILGRGQPLPENLQLAVQGKRSLPTMQQQPVNTGPYNRPPGMPMPHGGPASGPCPTPAMQTHAQSTGPKPWAEGQGAEAPAGPQKLPVPAPSGRPSPAPPQAPTAPQPIPSTSLTPQPQPPAQPQPQQQPQQGPGQPSLIIQLQQKQNRITPIQKPQGLDPVELLQEREYRLQARIAHRIQELENLPGSLPPDLRTKATVELKALRLLNFQRQLRQDVVACMRRDTTLETALNSKAYKRSKRQTLREARMTEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSISGKIQKLSKAVATWHTNTEREQKKETERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTTLVYEHKAAQAAKEKKKRKKKKKKEDGDAEGLGVIGPDGEPIDESSQMSELPVKVIQTETGKVLQGTDAPKSSQLEAWLEMNPGYEVAPRSDSEESGSEFEEEDEEEVVSRAESEEKKIIDPNSDEVSETAAKHIIESAKQDVDDEYSVQAGETSSQSYYGVAHAVIEKVEKQSSLLINGTLKQYQIQGLEWMVSLYNNNLNGILADEMGLGKTIQTIALITYLMEYKRLNGPYLIIVPLSTLSNWVYELDKWAPSIVKIAYKGTPSMRRSLVPQLRSGKFNVLITTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGERVDLNEEETILIIRRLHKVLRPFLLRRLKKEVESQLPEKVEYVIKCDMSAIQKVLYRHMQGKGILLTDGSEKDKKGKGGAKTLMNTIMQLKKICNHPYMFQHIEESFAEHLGYPNGIINGPDLYRASGKFELLDRILPKLQATNHRVLLFCQMTSLMTIMEDYFAYRNFLYLRLDGTTKSEDRAALLKKFNDEGSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEENEEEDEVPDDETLNQMIARNEEEFELFMRMDLDRRREDARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKIFGRGSRHRRDVDYSDTLTEKQWLRAIEDGNLEEMEEEIRLKKRKRRRHVDKGSRREDGEKAKKRRGRPPAEKLSPNPPKLTKQMTAIIETVINYRDGSGRQLSEVFVQLPSRKELPEYYELIRKPVDFKKIKERVRNHKYRSLGDLEKDVMLLCHNAQTYNLEGSQIYEDSIVLQSVFKSARQKIAKEESEDDSNDDDDDEEESEAESKSVKVKIKLSKREERSHDKGKKRTSRAKAKPVVSDDDSDEEQEDIFTRKMSNLRAAPQKTSDEDETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJ09</accession>
    <name>A0AAR2LJ09_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transposase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJ09"/>
    <dbReference type="Ensembl" id="ENSPNAT00000088039.1">
        <property type="protein sequence ID" value="ENSPNAP00000074759.1"/>
        <property type="gene ID" value="ENSPNAG00000033807.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271870"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057667">
        <property type="entry name" value="HTH_SB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25787">
        <property type="entry name" value="HTH_SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sleeping Beauty transposase HTH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="68"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="148"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="38184" checksum="546CE0978CF0617E" modified="2024-11-27" version="1">MKSKELSVDLRDKIVSRHKSGEGYRKITAALKVPMSTVASIIRKWKKFGTTRTLPRAGRPSKLSDRGRRALVREVTKNPMITLSELQRSSVERGEPCRRTTISAAIHQSGLYGRVARRKPLLSKRHKAARLEFAKRHLKDSQTMRNKILWSDETKIELFGVNARRHVWRKPGTAHHEANTIPTVKHGGGSIMLWGCFSAAGTGRLVRIEGKMNAAMYRDILDKNLLQSALDLRLGRRFIFQQDNDPKHTAKISKQWLQDHSVNVLEWPSQSPDLNPIEHLWRDLKMAVHRRLPSNLMELQRYCKEEWAKLPKDRCAKLVASYSKRLEAVVAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LXB6</accession>
    <name>A0AAR2LXB6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Telomere-associated protein Rif1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RIF1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017547918.1">
        <property type="nucleotide sequence ID" value="XM_017692429.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000058560.1">
        <property type="protein sequence ID" value="ENSPNAP00000078966.1"/>
        <property type="gene ID" value="ENSPNAG00000016685.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424396"/>
    <dbReference type="CTD" id="55183"/>
    <dbReference type="GeneTree" id="ENSGT00390000012204"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14267">
        <property type="entry name" value="Rif1_CTD_C-II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022031">
        <property type="entry name" value="Rif1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928:SF3">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12231">
        <property type="entry name" value="Rif1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomere-associated protein Rif1 N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1107"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1137"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1230"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1298"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1869"/>
            <end position="2132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2145"/>
            <end position="2175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2303"/>
            <end position="2323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1142"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1190"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1232"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1318"/>
            <end position="1330"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1332"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1375"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1393"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1424"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1449"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1470"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1541"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1559"/>
            <end position="1573"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1576"/>
            <end position="1590"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1627"/>
            <end position="1636"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1687"/>
            <end position="1704"/>
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        <location>
            <begin position="1889"/>
            <end position="1898"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1915"/>
            <end position="1941"/>
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        <location>
            <begin position="1942"/>
            <end position="1968"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1997"/>
            <end position="2013"/>
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        <location>
            <begin position="2061"/>
            <end position="2074"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2088"/>
            <end position="2109"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2154"/>
            <end position="2174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="2307"/>
            <end position="2320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2421" mass="263305" checksum="486E06AEEC910A69" modified="2024-11-27" version="1">MMATVPLSSSSLLPLLESLEDNAAGASEQTDAYLTIANRLSGEDGRQFFPIVVKHFSRLGKALQTHISSENEELSQAALQALGFCVFNTNIVSGISANFAEEILSALCALVLKSTDKNTCTRALWVISKQNFPAEVVSKKTLEILRTLDAMQTKDIQSILMDHESLNVVIKLLEQAPTQMGEGAVCWAKLVIPLVVHSASKVRLRAAAALEMGLPLLLEKQQEVAAIVEPMMSSKLIPEMQKLFSTKNESNVLKLWPLFVKLLGKLLHKGGAFINSLLYLEELGFRSSSPSIKKIAFIAWKSLIDNFALNPDILCSSKRLKLLMQPLSSILVRTEALLLTKLEVWWYLVVKLGPNLASNFDQVGVPLLHSTLSPDASVLQCPATPARGTSQCNGIGLATPKSGVPSCSSPGATSRSLNSSMSVPQSYTSIQLLGLEMLLHYFLGSQATAAATKNKLQLSLEPLTHNLLSSQSFFGKHSSTFISAVKSGFIAVGKDAPEALLSLIWNSLVNLVTATIETAGNKKERQGSEVLTLLLQALQSILSSEALPAPRALALLEATVKGIPQKVLGSAAYQVANMDVLNGTPALFLILLFYNNNLLSSFVADERFFVCLETLVGCGLCGPTSPLAFVEAVLGTISRNVEALENKEHLWRMWSVVVNPLTDTITQTNEVNQGDALEHNFSAIHSALMFPITHLLPGSALPQMMLKTMLSTWSRLYKAFARCSALVATAEENVCCEELCARISATLEGQALKNLSTLDAIANILLSVIESVDFSPFTPQFQQKMKSPHTPLGWVRKRNKALGNLSNFHVLLVQSLEAFLALNSPYEGSAVLSSIGVMLVSIVSTLFVNLSLPTVIQESLASLTKPLALLYGQVGSSEGEPSKSHSVIGSKLEKLVGEMLGCLQTRSSLQYDDELLAVLSPLLCVLFPHKSKHIRSAVTQFWNGTFGHALVLTYPEMLTPVLSQAKQKAPLILPGFQAVENAEDFSGPCSSECSQLDAEISGMKMSSAGKRESLLAKAAELKEKNSGTSKPVSVKLDFGSPKPPRREILEEEASVDFVFIPPEPKERVLTEHQKEIKRTKRDDIPAMYNNLDASLDTTVFTQYTQSQEESMDVLPADEQPKTTDTNKTETMGLEIEELPAGDAKEAQEEICSKTDAPEKVPNDANQATEVPSPVDISVADVSGHEDSAKIDQSPNVSGSSDMISGTPPKPSSRRQSFITLEKYAEGKPASPISVTKFTGPLTRTSCSPDIMKTQQSTPDAAYCSQPETDKKDAQASTFTAQHSMPQTEEKVEEIMAEVKSVVKRSSEGTEDEDDVIPDTQTQLLNGTVGDQSLDKFNVEVEPEKASLEGHSEVPAQSSSHGEPRRSGRRRSRPVRPGEDSVDQDSKNKLQKTDPVNESALTDAQKPTFTKQTESILPGRGRRSKVLEENKGESSRSQIGELKEHSQTDSQVSPSTTSNSSHGRRRKNKELSQTESPNKQSPLSQTDNTSDSDGPSHSRPLRRTRVSVGPEGSGKKQNGKDFQSDSQTGVSILKNKHADTLVSDTGADSQPTGRPKRTRRSEGTDAQNKDKPVIENESVSDLSQSDSQTFTPAKGRIGRPRKAGGEMNARKDTPPSDAINSESSQAEAVRDTKDSQGRGKYNTRRSSRGSLAPLETSESDGSDNREGQRSKKARGQRSLASEVIPDNMTHENVSESKMDKENADPIEMSEKEPKACLNVSMPCEAKEEVSIAKEPVIEHCISETKSTEQKSDESKMEVDAIKETGAVLEVKPLEDGTVASLQNSLVLPEKVVLKTPELHRCPHVKRSRGRRRSTSCNCLLASTSSAHSQEIVSSDSQVCEDVKDKVLLEFNSLPSESELSNIKPVFMEKHSETSPDSCPVVANDERSPVLLNSTDTGVPTESPEKELCATNTQLSDVKEGLTQAKEDQNAEDSEIKHPEKENSSTSENIYDQEQLQSSSTQGASQNHSATVDGKIECPDQSEAPVSQNNAIDLSLNEDGEKLEMPEKEDMHEMDDGVAPLPEGTELADAPCLKGDGEDTQMDDKDIQPETDLPSEVSMPLQMVSTETCLDSPTKQKTSDARQGDLDMGQSPSSSGTRGVWSPSASPSTSILKKGQKRQFEEDSPSPLLKSRRVSFANPIYQQELADDIDRRSPVIRTSSPRSKTVGQPKYITTPTKGLLTLSPRNLRSPGYKSSKKCLISEMSQEPRPIPKDCVYPALVCCSTPVEAVLPQISSHMWPRGLGQLVRAKNIKTVGDLSALTPSEIKLLPIRSPKLSNVKKALKNYHEQQRKGRSDDLKGFDEMEKMTSEPEEMDAAPNQEEEQTSKVATDVSDAQEVVSSEVASASEVSSDFLSALEQKQEAPVSVELLSDVSALGARLTSEELSRCSPRELGLMHEQLSGMMKSIVDHLQSRLVKSLEDSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M0K0</accession>
    <name>A0AAR2M0K0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcium-activated potassium channel subunit alpha-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">BK channel</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">BKCA alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Calcium-activated potassium channel, subfamily M subunit alpha-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">K(VCA)alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">KCa1.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Maxi K channel</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Slo-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Slo1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Slowpoke homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037400133.1">
        <property type="nucleotide sequence ID" value="XM_037544236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M0K0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065873.1">
        <property type="protein sequence ID" value="ENSPNAP00000080509.1"/>
        <property type="gene ID" value="ENSPNAG00000017117.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414676"/>
    <dbReference type="GeneTree" id="ENSGT00940000154935"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060072">
        <property type="term" value="F:large conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000098">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000005">
        <property type="entry name" value="calcium-activated potassium channel subunit alpha-1 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000015">
        <property type="entry name" value="Calcium-activated potassium channel subunit alpha-1 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048735">
        <property type="entry name" value="Slowpoke-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF33">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT ALPHA-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21014">
        <property type="entry name" value="Slowpoke_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01449">
        <property type="entry name" value="BKCHANNELA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="30">
        <location>
            <begin position="357"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="30">
        <location>
            <begin position="801"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="724"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1176"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="1180"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="28">
        <location>
            <begin position="1209"/>
            <end position="1218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00030518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00031999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00033447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1233" mass="137629" checksum="ED3D703A3FF3AF62" modified="2024-11-27" version="1">MTNLVSSEVSRGPARMDVVIPYSPDVPCDSNGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCSVKKKDVQRVSNPIGAGDGGGKVGDKKEAAAAVSEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSDPIESCQNFDKDITLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNSQPLSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPVVCELCYVKLKLLLIAIEYKSDQRESSTLINPGNHVKMQEGTLGFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRTRKAGSHRMRLACCLDCGQAERGCLCMSHRDHSNMETPPPRAFPLSSTSLSDKTAALHSSKSNYNGYIKSIEEVHQSTLSPKKKQRNGGMRTSPNCSPKTLRHDPLLIPSHDQMETMDENVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGLLQANSQGFTPPGMDRSSPENSPVHGLVRQTSVTTGANIPIITELAKSDRLLNAVCVRQDKSSGTNIPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEGLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSSPSQCTKRYVITNPPYDFELVPTDLIFCLMQFDHNAGQSRATLSHSSHSSHSSSKKSSSVHSVSTSTRAGRSKSREPRDKQNASRMTRGVAEDLVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M1X1</accession>
    <name>A0AAR2M1X1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M1X1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054614.1">
        <property type="protein sequence ID" value="ENSPNAP00000082810.1"/>
        <property type="gene ID" value="ENSPNAG00000031192.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="111" mass="12099" checksum="DF73BDD834EDF7BF" modified="2024-11-27" version="1">IFPKGVAAPTLTVLPPSSVELQQGKATLVCLANKGFPSDWRLSWKVDGSSWSSGVSQSPGLLQKNDGLYSWSSTLTLQEDQWLKKTVTCEATKDSQTPVSQTLRREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M3U8</accession>
    <name>A0AAR2M3U8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">RNF19B</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RBR family. RNF19 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017549837.1">
        <property type="nucleotide sequence ID" value="XM_017694348.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M3U8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045062.1">
        <property type="protein sequence ID" value="ENSPNAP00000081819.1"/>
        <property type="gene ID" value="ENSPNAG00000008914.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425590"/>
    <dbReference type="CTD" id="127544"/>
    <dbReference type="GeneTree" id="ENSGT00940000158451"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20355">
        <property type="entry name" value="Rcat_RBR_RNF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000001">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000004">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000052">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="334"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="387"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="104"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="108"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="592"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="611"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="654"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="703" mass="74507" checksum="7BEE080E9955FCF4" modified="2024-11-27" version="1">MGSEKDSESPHSSVSGIPNPKCRGPGKKQGRISFSSLFHSKRGSKGSKASAGAPLAQQLHQQQQLQQQLLLQPPTPTAASPDPPATSDSAEPLSSSQASLGGQDLLECPLCLVRQPRDQLAELQGCSHRSCLCCLRQYLRIEITESRVQLSCPECAERLAPRQVALILDDAALLEKYEEFLLRRCLASDPDCRWCPAPDCGFAVIASGCASCPRLVCRREGCGAEFCYHCKQAWHPNQTCDSARQQRALSLRTHSNHSPSYTAEQGPTDDIKPCPRCGAYIIKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYLSPSGCTFWGKKPWSRKKKILWQLGTLIGAPVGITLIAGIAVPAMVIGIPVYVGRKIYAHYEGKKTSQHRRNLAITGGVALSIITAPVIAAVSVGIGVPIMLAYVYGVVPISLCRGGGCGVSRGKGRGVRIDFDEDDGPITVADAWRALKSPSLGESSLEGAASGLSTTSPSEGLSVAPGGLGDTPHFNTLAGGALGAKTGKYSRLEVQGAELGKEGTRRETGSLGAASDCASTRGMAGSITSSYTLPDREGTNLEIQVDIETKPSHLCLTSEEDLAPPTAAVAPGSGEEPQDCSSRRGGILSSSSLGLSPGGSLREGLRDVTLAQPESIRSDLEMSDTQSDDIAELTSDDCDSPHPKSSRPPPLPQPTCRALNSTDGLHCPDNVILYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BKR8</accession>
    <name>A0A3B4BKR8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BKR8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000148"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014344.2">
        <property type="protein sequence ID" value="ENSPNAP00000000148.2"/>
        <property type="gene ID" value="ENSPNAG00000007303.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230358"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="19"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000148.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="142" mass="15701" checksum="046A692D2F9D89B0" modified="2024-11-27" version="2">MNWTGHVILESIPSDPVLVRPGGSFSVSCKAVGYDFGSYSMHWIRQPSGKALQWIGRIYTNTGTTAYTKGLEGRIEIMKDNSKSTSYLKLSGLTVEDTAVYYCARLAQCDKQMGRVYKNQQLSSLVMQGSVSLHPVQTILLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BND1</accession>
    <name>A0A3B4BND1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BND1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014346.2">
        <property type="protein sequence ID" value="ENSPNAP00000000150.2"/>
        <property type="gene ID" value="ENSPNAG00000007301.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244936"/>
    <dbReference type="OMA" id="GQRMESI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000150.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="101" mass="11078" checksum="934C52A8870B0447" modified="2024-11-27" version="2">QNMESLQTAVQLKPGETLSLSCRGSGFTFSSYNMHWIRQQAGKPLEWMARVSDGGGSKSYPASFQGRTEITRDNSNSMVSLKLSGVTVEDSAVYYCARSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BP83</accession>
    <name>A0A3B4BP83_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548412.1">
        <property type="nucleotide sequence ID" value="XM_017692923.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BP83"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000286"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014115.2">
        <property type="protein sequence ID" value="ENSPNAP00000000286.1"/>
        <property type="gene ID" value="ENSPNAG00000007414.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424729"/>
    <dbReference type="GeneTree" id="ENSGT01030000234599"/>
    <dbReference type="OrthoDB" id="8920155at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF59">
        <property type="entry name" value="APOLIPOPROTEIN L, 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05461">
        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="479" mass="52680" checksum="B77215D61E01378C" modified="2018-12-05" version="1">MSLGDNPKPKRPARPQALDPLTIQPTRPAPPPPTDLQSKIPRPPSEYPISPVYTNVLPAEVGTPSRPAPPIPRRPVLSREPCFSHSTETEDCTTPQNPLPAVRRPPAPPRPLARPRPGVVKPRRPFLSESLSEQPEEHIYEYIPDVLPDTPEDEGVNGGRGVCAVEMKPLTPHPLRSLSEQSSVDGDSSAKELDILLEWWRSVEGWEEVHMGHQREEPERKLMLVAQRVKMGMRLYQLLVFQHGRILLNHITELHCTADSLSRVGKNTRIAGITGGTTGAVGVAAVVAGVALAPITFGASLVAAGIGVGVAAAGGVTGASAAITSKVKSSQVRNKVERILKNYRFHMEDIIECLEFIDVGMEHIRKYNHSRLKNLDPCTLKIVQMAQEMGSFCSTDAIDKYSCILQGFASGLDSYFSEEDKLKLKKGSEHQFADNIREVAGKLKEGTDELIRIKNLFNSIVDHVSLNGPHFVEYCKAWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPD3</accession>
    <name>A0A3B4BPD3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PR domain zinc finger protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017548354.1">
        <property type="nucleotide sequence ID" value="XM_017692865.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037388100.1">
        <property type="nucleotide sequence ID" value="XM_037532203.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000000314"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014084.2">
        <property type="protein sequence ID" value="ENSPNAP00000000314.2"/>
        <property type="gene ID" value="ENSPNAG00000000180.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424679"/>
    <dbReference type="CTD" id="797296"/>
    <dbReference type="GeneTree" id="ENSGT00940000159410"/>
    <dbReference type="OMA" id="MASRCPS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19188">
        <property type="entry name" value="PR-SET_PRDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003271">
        <property type="entry name" value="PR domain-containing 2, with ZNF domain a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044414">
        <property type="entry name" value="PRDM2_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF37">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="6">
        <location>
            <begin position="24"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="465"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="1176"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="1204"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="1233"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="1396"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="1525"/>
            <end position="1552"/>
        </location>
    </feature>
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            <begin position="148"/>
            <end position="330"/>
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            <begin position="373"/>
            <end position="446"/>
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    </feature>
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            <begin position="1597"/>
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    </feature>
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            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
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            <begin position="198"/>
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    </feature>
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        <location>
            <begin position="508"/>
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        </location>
    </feature>
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        <location>
            <begin position="645"/>
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    </feature>
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    </feature>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRK1</accession>
    <name>A0A3B4BRK1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ANKRD40</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017561175.1">
        <property type="nucleotide sequence ID" value="XM_017705686.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRK1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001215"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012548.2">
        <property type="protein sequence ID" value="ENSPNAP00000001215.1"/>
        <property type="gene ID" value="ENSPNAG00000008028.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432087"/>
    <dbReference type="CTD" id="91369"/>
    <dbReference type="GeneTree" id="ENSGT00390000007792"/>
    <dbReference type="OMA" id="DYTEDTQ"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039195">
        <property type="entry name" value="ANKRD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24192">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24192:SF3">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="401" mass="44770" checksum="3104377DED5DDF7D" modified="2018-12-05" version="1">MSTSSLDKELQERLREASAIGDIDEVRTLVESGVNINSQNEINGWTCLHWACKRNHKHIVAYLLNSGADKEILTAKDELAVQLTSKPEIRRLLGVEEEEEDVPELKEPELPIIPNYLSNPPFLYGKMDKDELNLARHVSQNGTSDHSESVDSEPATLSPTQEQQQPPQQQQLPQSLYAESQSQQAVSYISVVEQNGIVPSPVVNRSLSMEVSPEAHHGNHSEFTHNHTIAQNGPVCPPLPSPGAGTNQASVSNPTVTRQQSLPQQLNCSQPGGSMPAFQPFFFTSTFPVNVQELVLKVRIQNPHARENDFIEVELDRQELTYRALLRVCCRELDISAEHVEKIRKLPNTMLRKDKDVARLQDFQELEVVLEKAEGLSLFSGGPGGLTDRPCYNMKASRLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BU71</accession>
    <name>A0A3B4BU71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BU71"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003173"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009168.2">
        <property type="protein sequence ID" value="ENSPNAP00000003173.2"/>
        <property type="gene ID" value="ENSPNAG00000034122.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282554"/>
    <dbReference type="OMA" id="IAWIQEG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="222"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003173.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="607" mass="68758" checksum="6A489DD056E07CE0" modified="2024-11-27" version="2">MELDSLTDYLENTLRSTLENVAPLKIKRIRQKKFAPWYNDKTRTLKQTARKLERKWRHTKLEVFHTAWKDSLIEYRNALTEARSPYLASLIENNKYNPRVLFNVISQITQNQAGTEPEIPATLTSEVFMDFFNNKIDNIRQQIQPTVSNSNPAWLSSDLADIEQNTAVEKNLETFYPLPQLELEKIISSANCTTCTLDAIPSKLLKEVLPAIINPILTIVNSSLSLGHVPKAFKLAVIKPLIKKPNLDVTVLSNYRPVSNLPFISKILEKAVAQQLSSYLHKNHIYEKFQSGFRPHHSTETALVKITNDLFLASDQGCVSLLVLLDLSAAFDTIDHNILLERLENMVGVTGTALSWFRCYLSERYQFVRVNNLSSTYSKVRFGVPQGSILGPLLFTLYMLPLGTVISNHGVNFHCYADDTQLYISAKPDDQYRLKKMEDCVKDVKGWMSQNFLLLNSNKTEVLLLGPKLARSKSPDLILNLADFPATPGSAAKNLGVIIDSDLSFDQHIGSITRTAFLHLRNIAKIRNALSLRDAETLVHAFITSRLDYCNALLSGCTSRNLNKLQLVQNAAARVLTKTRKFEHISPVLSSLHWLPIKFRIDYCRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVK6</accession>
    <name>A0A3B4BVK6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Upstream stimulatory factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Major late transcription factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Upstream transcription factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">USF2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVK6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002530"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010280.2">
        <property type="protein sequence ID" value="ENSPNAP00000002530.2"/>
        <property type="gene ID" value="ENSPNAG00000005658.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160704"/>
    <dbReference type="OMA" id="TANTRMA"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18923">
        <property type="entry name" value="bHLHzip_USF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000045">
        <property type="entry name" value="upstream stimulatory factor 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF2">
        <property type="entry name" value="UPSTREAM STIMULATORY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="225"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="203"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="277"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002530.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="336" mass="36642" checksum="FB7912827DFB5F52" modified="2024-11-27" version="2">SALPSPYFSVAEYHVLNRLPSWICSNRAWTRRRGEGVGAEEPTAVTIASAQQAAVFAADHNVQYQFRTENSGGQVTYRVVQVTEDQLEAAGDGGAAVSVVSTTTFAGAQQAVAQAVIQNPFSNGGSPAGETVGGETRFAYFPAATVSDGTATAVSVQAATDSALTPAGGQFYVMMSPPDVLQTGTPRTIAPRTHTYSTDFGENEMLQHGSSNWKVDGPRAPRDERRRAQHNEVERRRRDKINNWIVTLSKIIPDCNMDNTKTGASKGGILSKACDYIRELRQTNQRLQESFKEVERIQVDNELLRQQIEDLKNDNALLRAQLQQHGLEISGDTTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVU6</accession>
    <name>A0A3B4BVU6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568778.1">
        <property type="nucleotide sequence ID" value="XM_017713289.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVU6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008209.2">
        <property type="protein sequence ID" value="ENSPNAP00000003748.1"/>
        <property type="gene ID" value="ENSPNAG00000010156.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436665"/>
    <dbReference type="CTD" id="335651"/>
    <dbReference type="GeneTree" id="ENSGT00510000047107"/>
    <dbReference type="OMA" id="VMMLLAC"/>
    <dbReference type="OrthoDB" id="9923952at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034632">
        <property type="term" value="F:retinol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00743">
        <property type="entry name" value="lipocalin_RBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000005">
        <property type="entry name" value="Retinol binding protein 4, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022271">
        <property type="entry name" value="Lipocalin_ApoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002449">
        <property type="entry name" value="Retinol-bd/Purpurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873:SF1">
        <property type="entry name" value="PURPURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036893">
        <property type="entry name" value="Lipocalin_ApoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500204">
        <property type="entry name" value="RBP_purpurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01174">
        <property type="entry name" value="RETINOLBNDNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017107656" description="Lipocalin/cytosolic fatty-acid binding domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="8">
        <location>
            <begin position="41"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="24"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="90"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036893-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="196" mass="22132" checksum="CD0DE4697FD76EBD" modified="2018-12-05" version="1">MEYYKFALLVVFLSYIERCWSSSCVVDSFTVKQDFDPKKYAGKWYAQQKKDPEGLFLQDNISAEYTIDEDGTMTASSKGRVTLFGFWVVCADMAAQYTVPDPSTPGKMFMTYQGLASYLSSGGDNYWVIDTDYDNYAITYACRTLKDDGTCEDGYALVFSRNPRGLPPAIQRIVRHKQEEICMAGQFQPVLQSGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWU7</accession>
    <name>A0A3B4BWU7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWU7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004162"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007503.2">
        <property type="protein sequence ID" value="ENSPNAP00000004162.2"/>
        <property type="gene ID" value="ENSPNAG00000001411.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286911"/>
    <dbReference type="OMA" id="EICHIAN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000100">
        <property type="entry name" value="Si:dkey-11n14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="25"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="772"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004162.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="967" mass="109425" checksum="816C68CCE0BE9187" modified="2024-11-27" version="2">MQKCQDTPINCNDIFSLPWKNEENKVVLTKGIAGIGKTVSVHKFILDWVEGEANQDIDCIFLLPFREINLIKPGEYSLHELLQEFHPELEELNATKMYEACKLGFVFDGLDESRLPLDFDSRNVRSIKKKATVDALITNLIKGNLLPSSVIWITSRPAAANKIPSQYVSLFTEVRGFTDKQKEEYFKKRIADETEALKIISHIKTSRSLYIMCHIPVFCWITATVLQAMLVENHGENIPTTLTEMYIHFLLIQMNMKNQKYDQKVERDLKKLLESNRGIIFKLAKLAFEQLKRENIMFYEDDLRECGIDVRGDSEYTGMCTEIFKQESVLHEKKVYCFVHLSIQEFLASLHVFYCYLNKDRDELQFLLEGPLPEGFTFIVDSPKTLSLYYLLVKAVDKSWRSQRGYLHLFLRFLLGITLDSNQKLLKGLLAQTEDSKETVEKSIRYITKIQRKDISAEASINLLFCLLELKDRTLFKQIQTYLSSEETPETELSLSVCSALVYILQMSEEILDEFNPKMYNTSNAGCRRLVPAVRCCRKALFNSCGLTEQCCETVASALQLPNSPLRELDLSNNSQMDAGAKLLSEGLMSPQCKLEKLSLASCHFSKEQCEVIALAVNSASSPLRELDLSYNDLQDSGVKLLSDGLKTSDSKLEKLRLGWCKLSADSCKTLTSVFSSSSPLTELDLSNNDVQDSGVKFLSAGLENPNCKLEVLRLSGCLVTEKGCSHLASALISNPAFLRELDLSYNHPGHLGTKLLTGKLEGPQCRLQILNIDSGGECRIKSGPRKYTCEITLDPNTAHRNLRLSERNQTATRVLELQGYEPNPERFDQCIYVLCRESLSDRCYWEVQFSEDLAGVALTYKGIERNGRGDDSTFGNNRMSWQLVCFGSGSTYCAFHNNNRTEIPALSFYSNRVGVYLDWPAGILSFYSVSSDTHALTHIHTFYSTFTEPLYVGFCFGNAGSVISLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZ10</accession>
    <name>A0A3B4BZ10_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fibroblast growth factor</fullName>
            <shortName evidence="6">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567608.1">
        <property type="nucleotide sequence ID" value="XM_017712119.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZ10"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008167.2">
        <property type="protein sequence ID" value="ENSPNAP00000003756.2"/>
        <property type="gene ID" value="ENSPNAG00000036000.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108435957"/>
    <dbReference type="CTD" id="65089"/>
    <dbReference type="GeneTree" id="ENSGT00940000159518"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042664">
        <property type="term" value="P:negative regulation of endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000007">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003756.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="199" mass="23022" checksum="8506070D9CF13CDF" modified="2024-11-27" version="2">MTGNMKACDVTTMQSVSVPNFKHHVTEHSRLSDRMSRRLTRTYQLYSRTSGKHVQVLGNKRVVANGEDGDAHAKLVVETDTFGSRIRIRGAKTGFYICMNKKGKLIGRRKGHGRDCIFTEIVLENNYTALQNAKYKSWYMAFTRKGRPKKATRTMQHHREAHFMKRLPKGHLLTERKPFDVIPYQLNKRTKHSHRPGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZ58</accession>
    <name>A0A3B4BZ58_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zgc:153911</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZ58"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003870"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007967.2">
        <property type="protein sequence ID" value="ENSPNAP00000003870.2"/>
        <property type="gene ID" value="ENSPNAG00000010303.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154641"/>
    <dbReference type="OMA" id="QYVVLDC"/>
    <dbReference type="OrthoDB" id="5857426at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="96"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="40"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003870.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="360" mass="40329" checksum="02245DB36A76F359" modified="2024-11-27" version="2">MVIAFFVICLKGKKKFSPLTADCLVYCSSAVLKMAPLQPRLEKEKPKQSRGTPERGAPDWDGGEMERRRDEERSIKTTKAEFEISVPSGGQVGVYGQSVVLFCSFPTGGSWDVSSSVITWQRGLEVVHSFYHSQDQLDRQHPRYANRTSLYHQEMAEGNASLRLDRVSLEDHGIYTCSVSTQIGSQKKSFRLRVAAFYPEPHLHISLSSNGQVDLLLTSQGGYPSPSLQWLMGNTQDVTEETQTQLEQDQHTRLYNVNSKLNLTRGTNSSITFILKNQELGQEIRRNIDLFLEDGAGGPQSRHRVHSVLLGAVVTVAVGLAVLIVAFFIRKQKKQKRDFRAVSAPDEEQSINHQNGKTVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4P0</accession>
    <name>A0A3B4C4P0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">KIND domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the spire family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548817.1">
        <property type="nucleotide sequence ID" value="XM_017693328.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4P0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005549"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005102.2">
        <property type="protein sequence ID" value="ENSPNAP00000005549.1"/>
        <property type="gene ID" value="ENSPNAG00000001932.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424974"/>
    <dbReference type="CTD" id="557962"/>
    <dbReference type="GeneTree" id="ENSGT00390000003058"/>
    <dbReference type="OMA" id="EEDSPNH"/>
    <dbReference type="OrthoDB" id="10043757at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
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        <location>
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    <sequence length="780" mass="88428" checksum="DD362C102F99D1A9" modified="2018-12-05" version="1">MAKRPGNDDDGGTLTLISPTVGQDITDGAEDTAEALSLEEILTLYSQPINEEQAWAVCYQCCRFLAPRRRTKSAKSPARRIEGPGDVRIRKDGTVHLNRQSSPDKHTRLSTSSTEVIESLGIMIYKALDYGLKENEERELSPPLEQLIDLMTNMTDTESDPCPDEGYEATEEEDEGEEDEGKEEPEEVSSIRGYRDIIMLCTSHLPSPLDAPNHYQAVCRALYAETRELRTFLEKIKSAKENLRRMEGETPEEPVRDLNELQNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPHPPRPPSLHERILEEIKAERKLRPVSPDMIRRSRLVMRPLSMSVSFDSSGAGKSMSTPQDLFRSSDLSIPDGPRKLAVSTLTLANGTPSAHSPVNGVAGAQPLSQRKRLLKAPTLAELDSSDSDEEPSTRRSVSSSSMSTSLVDDTSPESVTGKKSPPQFLPISSTPQPDRRTAPQRRHSIEKEAPTSVRPFVPPSRQSSRSLLPSEKKEEFCYPVECLALTVEEVMHIRQVLVKAELEKFQQYKDIYNALKKGKLCFSCRTKRFSIFTWAYTCQFCKRPVCSQCCKKMRLPSKPYANLPIYSLGSTTLPKEETGAAAQPEKASGSHHRHSLNRSISRFSKHGERSFSKDEVELPKELTEDWSTMEVCVDCRKFINDIISSSKRSLSLATKRARLKRKTQSFYMSPSNSRDFRPSERTINEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6B8</accession>
    <name>A0A3B4C6B8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C6B8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002167.2">
        <property type="protein sequence ID" value="ENSPNAP00000007243.2"/>
        <property type="gene ID" value="ENSPNAG00000013110.2"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="Spore_Coat_Assembly"/>
    </dbReference>
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        <property type="entry name" value="LP04239P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35365:SF18">
        <property type="entry name" value="MUCIN-19-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007243.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="159" mass="16999" checksum="162564E7D57C1D2A" modified="2024-11-27" version="2">LQSVQTECCGLQSVQTECCELQSVQTECCGLQSVQTECCGLQSVQTECCELQSVQTECCELQSVQTECCELQSVQTECCGLQSVQTECCGLQSVQTECCELPSVQTECCELPSVQTECYGLQSVQAECCELQSVQTECCEVPAAGGWGVCTDVCAGSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6L1</accession>
    <name>A0A3B4C6L1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017556201.2">
        <property type="nucleotide sequence ID" value="XM_017700712.2"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSPNAT00000001730.2">
        <property type="protein sequence ID" value="ENSPNAP00000007497.2"/>
        <property type="gene ID" value="ENSPNAG00000013288.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429164"/>
    <dbReference type="GeneTree" id="ENSGT01110000268427"/>
    <dbReference type="OMA" id="GIECIYL"/>
    <dbReference type="OrthoDB" id="9898017at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF130">
        <property type="entry name" value="BUTYROPHILIN-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="467"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="561"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="145"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="386"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="471"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007497.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="616" mass="71590" checksum="136D78D069D40EE9" modified="2024-11-27" version="2">MFILVCRYNRVFAYPSDDVTLTSHLSPETSAVGMEIRWFRGIECIYLYRNGQVTVGRGYEGRLSLFNEELQIGNVSLLLRDVGQADTGIYSCQVITGENKENRVEKTIHLYMAGTEIVSPDRSGLKIAEQDSLDMEESVHSLEMKKLLQKKDKELQDKIKELESATDKLRRMTSLLQDRETDLDNMRKVSNAGEASLKNMAGELEACKEELEKLGLKIQEKDNQSKVMLEELQARTRAVQVLTVTLQEKERELEEEKKQQEEREQLLQTVNEAGPGTANAPQAPVVSELVEKSEEMLREMYQDPKDQLEDIERWIHTKREERRSREEEESTEALEKEFFNYVLMIKGQIEEEHGKIVTLAKYIADLQEAYEHVATDEERGELEVYLKEALERQREGEGEIERLTKEIDKQRNAVEEKHKHEVEQIQEKYEVVSRIEAETRLLKIILPDVQAYFRNITAEVHRVFTKQMQGTDREKELETGGEREREPDREETSVREKMQEDRMSPIEEESSNEILEVQEQRMEPEQARHIVVGLGNENSVETLAQGTQADARVQREINEEIQKSEGTHSESETLELEENPSLVNGVMTLRQEEVQVELRVREHDEERNEVDMDYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7H2</accession>
    <name>A0A3B4C7H2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C7H2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007613"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001532.2">
        <property type="protein sequence ID" value="ENSPNAP00000007613.2"/>
        <property type="gene ID" value="ENSPNAG00000013387.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164012"/>
    <dbReference type="OMA" id="SEHYGKT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF135">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING 3-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="168"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007613.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="294" mass="33360" checksum="CDBCA31C4163A4EF" modified="2024-11-27" version="2">MAFVVKKLSIALDPVNASNTFTNGDCLSGRVTLEVSRETRIQCFSLKAKGKASVLWSEHYGKYSTVVYHDKETVFKSVHQVCNENGSVLLGLHSTNLIFFFVYRDMPASFKGRHGKVDYFLEAKLCRSMQISRKAKIQFNYVPQGDLSMPDLTMPQCGNTEKDMKFFSSGNVAMNIHTERMGYRLGEGLKVQIYIENNSSQRVKPKFCFYQKQSFFALKKRKLATKKLLKYKGDAIEPSAKTTVTKVLIIPEDTCVSILNCRVLKVEYRLKVSYSVLAFSPTFLSNIVMSNSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CA71</accession>
    <name>A0A3B4CA71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 229B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM229 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546110.1">
        <property type="nucleotide sequence ID" value="XM_017690621.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CA71"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007314"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002033.2">
        <property type="protein sequence ID" value="ENSPNAP00000007314.1"/>
        <property type="gene ID" value="ENSPNAG00000002586.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108422651"/>
    <dbReference type="GeneTree" id="ENSGT00390000010899"/>
    <dbReference type="OMA" id="VWDWCST"/>
    <dbReference type="OrthoDB" id="5946847at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010540">
        <property type="entry name" value="CmpB_TMEM229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06541">
        <property type="entry name" value="ABC_trans_CmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21169" checksum="279AEC22140F0030" modified="2018-12-05" version="1">MEFRTQLVNRGDSKSYNSGFETNTPTDVYGQVTLLPLSPLARLYIYALHGCACEVAFTAAWNWYYTRDQRLPGSTSLWALLIYSSAIFLMEFMSARLHQRHCPLLLRATVYTLFIYLWEFSWGFLLRLLEACPWDYSDFKYNLIGLVTLEYAVPWAFAALLAEKHVIGNTLKIRLNAESILSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CB77</accession>
    <name>A0A3B4CB77_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Aminopeptidase</fullName>
            <ecNumber evidence="18">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ANPEP</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="16 18">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16 18">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CB77"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008136"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000660.2">
        <property type="protein sequence ID" value="ENSPNAP00000008136.1"/>
        <property type="gene ID" value="ENSPNAG00000014278.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154876"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000005">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000012">
        <property type="entry name" value="Aminopeptidase N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF300">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 18" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 18" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 18" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 16 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="21">
        <location>
            <begin position="93"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="19">
        <location>
            <begin position="326"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="20">
        <location>
            <begin position="620"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="971" mass="111475" checksum="6CA496B2CC4CA16F" modified="2018-12-05" version="1">MGKGGFVASVVGTIAVIIIIGLSVGTQKHKQEESRTKVMPKVEVKPKTAVMPKTVVKPKTAVKPKTVVKPKTEDDPIHEAKLWKNYRLPTSLVPLYYNVTLWPQLKMDIYGRYIFIGHSGAAFMCVNETDLILIHSYELKYSLTWGRHHAMLRGLSGAKAPAIKKIWLEVETQYLVVQLSEKLQVGMTYWLYTEFQGELADDLRGFYRSEFFENGVKKVVATTQMQPTFARKAFPCFDEPAMKAVFHMTLIHPLRTVALANATEIIFHNKKLVLQTKFEPTEKMSTYLLAFVVSDFISIKSPAEAKVLVRTIWAQRKAIEAGQGKYALNITGNIINFYQNYYNSTYPLQKSDQIALPTFSFGAMENSFKEKALLFDPSMSSNQDHEWALMANSHEVAHMWFGNLVTMKWWNDLWLNEGLATYVSYLGADDVQPTWNMKDLMVLYEVQKAFALDAVASSHPLSSPEEKVNTPAEIADQFDYITYSKGAAVLRMLSEFLSEPVFARGLSAYLKQFAYGNTVYTDLWGNLQEVTVNLPASVTEIMNRWILQMGYPVVTIDTQTGRISQEHFLLDSESEPDRPSLYNYEWFVPIKWMKNGTVMGQHWLLEKTATHEPMRTNSEWVLANVNVSGFYRVNYDSQNWQRLISQLTSDHSVIPVINRAQILDDAFKLARAKIISITVALSITKYLSEEREYMPWESAIKKLDHFFLMFDWTEVYGAMEEYLRGKVQPLFNYFKNITADWTEIPSSHTDQYNQVNAISLACITGVEGCVNLTKTWYRQWMENPDKNPIHPNLKMTVYCNAIAAGGVEEWEFGWNMYKSSSSAAEAEMLLYGLSCSKDLSLLSRYLKYTLGVNMIRKQDATSAILYVANNKHGHLLTWFFIRDNWDHIIYDFVCCRGGLFSFSRIILGVSKRFSTLFELIWLRQFREELLYSGFINTAFVVHHAIETTKANMKWVAQNKKEVMRWLTEESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDZ9</accession>
    <name>A0A3B4CDZ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IRS-type PTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CDZ9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010167"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016744.2">
        <property type="protein sequence ID" value="ENSPNAP00000010167.1"/>
        <property type="gene ID" value="ENSPNAG00000015682.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160725"/>
    <dbReference type="OMA" id="VKLMVQE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000110">
        <property type="entry name" value="Docking protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF45">
        <property type="entry name" value="DOCKING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="7"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="2">
        <location>
            <begin position="130"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="28158" checksum="F9CA391CD2038CA2" modified="2018-12-05" version="1">MSSGFNDIIKQGRVTIRSQHFRIYQKCWLVMKRASSEGPFWLEKYSKQRSTNTKSPQKVMGLTNVKNVSRSPRQQKKHAVMLEFREESSLTFACKSEAEADEWYNVLHIYCIQEKKKKLDFGEPDLLCHIRNEALQYHVFLLPSDRFTAQGECTLQITHKAIHLYNAQDPRQRIASWPLKVLRRYGRDCTCFTFEAGTMCDTGEGLFRFLTAEGEAIYHRVHTAVLAIMESHCSRLVKTQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFJ9</accession>
    <name>A0A3B4CFJ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017566395.1">
        <property type="nucleotide sequence ID" value="XM_017710906.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFJ9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010243"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016845.2">
        <property type="protein sequence ID" value="ENSPNAP00000010243.1"/>
        <property type="gene ID" value="ENSPNAG00000015761.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435230"/>
    <dbReference type="CTD" id="149951"/>
    <dbReference type="GeneTree" id="ENSGT00390000012419"/>
    <dbReference type="OMA" id="SQQWGEH"/>
    <dbReference type="OrthoDB" id="76101at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04755">
        <property type="entry name" value="Commd7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047155">
        <property type="entry name" value="COMMD4/6/7/8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037358">
        <property type="entry name" value="COMMD7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF2">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="133"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="200" mass="22817" checksum="2BDC8CF8DE0E14F4" modified="2018-12-05" version="1">MLQLHFTNDALPDSVSNDFQTLNKFSEQQFVSLTELLYQFLLEPKESERFLAQLSEFAGEHSMGAGPLRGLMKSILLLPHGALKKNLTAEQVKEDMLSLGLNEDKAIHFSDQWRVHYPILSRLAVGQTLMVNQLVDMEWKFGVTVGTSELQKAGNIFLQLKLVVRKGNTTENVYMELTLPQFYNFLHEMERAKASMDCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGA6</accession>
    <name>A0A3B4CGA6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017559032.1">
        <property type="nucleotide sequence ID" value="XM_017703543.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGA6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009489"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035347.2">
        <property type="protein sequence ID" value="ENSPNAP00000009489.1"/>
        <property type="gene ID" value="ENSPNAG00000014969.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430817"/>
    <dbReference type="CTD" id="565419"/>
    <dbReference type="GeneTree" id="ENSGT00940000156912"/>
    <dbReference type="OMA" id="CEPRGDT"/>
    <dbReference type="OrthoDB" id="10025005at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18414">
        <property type="entry name" value="BTB_KCNC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000011">
        <property type="entry name" value="Potassium channel, voltage-gated Shaw-related subfamily C, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000014">
        <property type="entry name" value="Potassium channel, voltage-gated Shaw-related subfamily C, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily C member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003974">
        <property type="entry name" value="K_chnl_volt-dep_Kv3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005403">
        <property type="entry name" value="K_chnl_volt-dep_Kv3.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF87">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01581">
        <property type="entry name" value="KV31CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01498">
        <property type="entry name" value="SHAWCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="413"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="21">
        <location>
            <begin position="8"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="120"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="130"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="603" mass="67503" checksum="18A4D2BE69100D28" modified="2018-12-05" version="1">MVQGDEKDRVVINVGGIRHQTYRSTLRTLPGTRLAWLAEPDAHSHFDYDAQIDEFFFDRHPGVFAHILNYYRTGKLHCPADVCGPLYEEELAFWGIDETDVEPCCWMTYRQHREAEEALDSFGGGPAEMGNDDTEGELGDPGDGDEELEMTKRLALGDAPDGKAGGLWQRWQRRVWALFEDPYSSKYARWVAFASLFFILVSITTFCLETHEAFNPIINRTDIDPLDNSTRVYQETETEVYLTYIEGVCVVWFTFEFLMRITFCPDKKKFIKNALNIIDFVAILPFYLEVGLSGLSSKEAKDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGAKPNDPRASEHTHFKNIPIGFWWAVVTMTTLGYGDMYPQTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKKNKHIPRAPQLGSPNFCKSGMNSPHHSTQSDTCPLAAQDEVLEMNRADPKVNGEAAKAALANEDCPHIDQAISPEDGQIFSPCEPRGDTPCFLLNMAGRPTHTGTRVRKAGTFSGRSGGEPAGPTLGPQGYPLLQLGWTHSQEDGHARVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJ74</accession>
    <name>A0A3B4CJ74_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Inner centromere protein ARK-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Centromere</location>
            <location evidence="4">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the INCENP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJ74"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011415"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018373.2">
        <property type="protein sequence ID" value="ENSPNAP00000011415.2"/>
        <property type="gene ID" value="ENSPNAG00000017123.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111073"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990385">
        <property type="term" value="C:meiotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051257">
        <property type="term" value="P:meiotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051310">
        <property type="term" value="P:metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022006">
        <property type="entry name" value="INCENP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005635">
        <property type="entry name" value="Inner_centromere_prot_ARK-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13142">
        <property type="entry name" value="INNER CENTROMERE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13142:SF1">
        <property type="entry name" value="INNER CENTROMERE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03941">
        <property type="entry name" value="INCENP_ARK-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12178">
        <property type="entry name" value="INCENP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome passenger complex (CPC) protein INCENP N-terminal" evidence="19">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Inner centromere protein ARK-binding" evidence="18">
        <location>
            <begin position="658"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="46"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="484"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="653"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="494"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF03941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011415.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="84001" checksum="9CBE94527F7C4EE5" modified="2024-11-27" version="2">MSSLPEAMRSLTQVFTGKLQDFTDEVENVHMLWLEEIQQEAQRMFSSDFSTEPELMPKTPSQKKTSRRKRVSVELNESRSKRRFSKGKRSNLRRSSVQNTLNSISELVVSHAPSSSPDTVAEEPVRRTRRNKAAAADSEPVKRSTRNKAAGKAKEQAEVMEEEAVEVAAVADAPQPPDVPENVLEVKESNSSSSPVQTLLSQAVVKIPSAERLSANKLLESGPSPGRSANKITIAAAGAQASSRTSVRRSLVLRHSLVGLRHSMTQEAVRRASRRSFLKKKARLGNSTCSSSVSGETPHMQKCTHLISVSFRASRSGSKRRAAESAAIALSTPKKKHSPPKKCMTSITPSMRFLQTVQKNQLLMMTPSSLGRSSIMKSFIKQTGKTDIKVSLFLSHTTQLICSHRQADKKRDERLKRVVEARVKGEKEKEQEKKKKIEEKMAQIEKKHDLLRVERLAEEKAKKKVATKRQEELELRRKQEEAARKKKLQQAEDEERRHQELLAKRKAEEEREKARKQAEAARALELKKEQERERERERQAAAERERVEREKAIALQKELDRAAKEKERRELEKKRVGILLTVLRVIHLTTVLCGGLAHPRPVFCSLATCVFLPWLNWPFPGAGLNVTVDVEQSPQSYQITPKGKKIDVLVNPEDYGMDQNSDDSTDDESAPRKPIPSWAEGMQLQQAIMKRYYDPLDLDSYFGEIEQPRLEKIFQKTKPRFFKRTSSAVWHSPPRMGNMPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CK29</accession>
    <name>A0A3B4CK29_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Basal body-orientation factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Coiled-coil domain-containing protein 176</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">BBOF1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BBOF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CK29"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010879"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017658.2">
        <property type="protein sequence ID" value="ENSPNAP00000010879.1"/>
        <property type="gene ID" value="ENSPNAG00000016479.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435776"/>
    <dbReference type="CTD" id="565282"/>
    <dbReference type="GeneTree" id="ENSGT00940000154427"/>
    <dbReference type="OMA" id="MEADKWT"/>
    <dbReference type="OrthoDB" id="441129at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032777">
        <property type="entry name" value="DUF4515"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845:SF5">
        <property type="entry name" value="BASAL BODY-ORIENTATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14988">
        <property type="entry name" value="DUF4515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4515" evidence="12">
        <location>
            <begin position="85"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="53"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="274"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="521" mass="60204" checksum="338DAAEAE2E16B35" modified="2018-12-05" version="1">MPKKKGKKGKKAKGKGQKEGKHELKLDKESDVERAKANAALWEARLEVTEGSRVEYREAARRLARANEELTNQQYRSEKDTIDIIAFLKKKDLEKEAKIVALEEQLKEQKSKALQEKELVVAEYMQKIDELEEKFKKRSNDFRMIQGELKNIKEFHKKKAQMEHELSTIKESMYIADREHKDSLARMEHKFFSEKVRLEKEAEQRIAQLAEKAHNEAIVQMDDASRSVFKENVRLNEALGYHLKEVEDLRKTNEALAKENTSLTLHKEMSELMMKENVAQLAAQRSEIAELRAKVATLEQALSIMAGEFEREKAAVRERAVVSTQTSSVELEKLQKLLSMREHEMSRVKRLAHRIVEQRTQLELFFHEALAQVKQEITASQLQYRQKAMEAYRRRMSEARTGRTEYPRIRTFNKAPYSTNNVYTDLEEAEKWNNLQSSKVDISELTWEQKERVLRLLFAKMNGLKIRKAAEPLALTASSKRDRSGSDPGVTEGESHATFITQAVVSNITSNPSSLPDLQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTH5</accession>
    <name>A0A3B4CTH5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTH5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021522.2">
        <property type="protein sequence ID" value="ENSPNAP00000013829.2"/>
        <property type="gene ID" value="ENSPNAG00000019881.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="OMA" id="EDNIWIF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043792217" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="17"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="31"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="128"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013829.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="326" mass="36489" checksum="4EE37847DF6E2869" modified="2024-11-27" version="2">MITVLTAFCIFVSVQTSGFDDLRVQAVKYKENVTIKCEQSFVKGHKHYLAWYKQSFGKLPQFIARAAKHNEDARYARPFVERFRLTATEETFDLKIMNTVEEDTGTYFCVKVKEYVTEFGSGILLLFPDEKSEKRNLTEVDVKSGESVTLQCSVAPLSCSGDHSVYWFRHGSGEPHPGIIYTRGDTDSRCTRSSETDSPTQSCVYKLPKRNLSLSDAGTYYCAVAACGQMLFGSGTNLCIKGDNSQTVIALATSNVITVIVTVILAVLLLKNQWKGSFNDHPNHTNQVANTDALNYAALNFAMKPRPFRTSRVKECQDIYSQVKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTQ8</accession>
    <name>A0A3B4CTQ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">alcohol dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="5">1.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">S-nitroso-CoA reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids. Acts as an aldehyde-detoxification enzyme. Also acts as an inhibitor of protein S-nitrosylation by mediating degradation of S-nitroso-coenzyme A (S-nitroso-CoA), a cofactor required to S-nitrosylate proteins. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADPH-dependent reduction of S-nitrosoglutathione. Displays no reductase activity towards retinoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-nitroso-CoA + NADPH + H(+) = sulfinamide-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:78375"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145546"/>
            <dbReference type="ChEBI" id="CHEBI:145548"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:78376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-nitrosoglutathione + NADPH + H(+) = S-(hydroxysulfenamide)glutathione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63500"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTQ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015472"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023683.2">
        <property type="protein sequence ID" value="ENSPNAP00000015472.2"/>
        <property type="gene ID" value="ENSPNAG00000021493.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166411"/>
    <dbReference type="OMA" id="PRISFWR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000006">
        <property type="entry name" value="Aldo-keto reductase family 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="14">
        <location>
            <begin position="22"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="13">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015472.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="322" mass="36379" checksum="E07250BF1FD6C7F9" modified="2024-11-27" version="2">MSNGQPKNIKLRDGSFMPLLGQGTFNLKVATSGRSQEILEEAIDAGYRHLDTAIVYQTEVDVGKAVRSKLQQGVIKREDMFIVSKLWCRYFAPEAIPVCLDKSLSDLQLDYIDLYLVHFPVGLQDVGDDLFPKKDGKTLTTDFDYVDVWKGMEALKATGKVKSIGVSNFSVSQLERLLSVAKVPPVVNQVELHPYLVQTDLIKYCKSKNIALTAYCPFGSPDKPKEMHRGETDPEKLLEDPVVGTIAVKHKRTPAQVLLRYHIEQDIAVIPKCVNSIQILENKKVFDFSLDEEDMTTLRSLNRGWRAAPFNEMKSHPFYPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTT5</accession>
    <name>A0A3B4CTT5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RGS4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTT5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015502"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037738.2">
        <property type="protein sequence ID" value="ENSPNAP00000015502.1"/>
        <property type="gene ID" value="ENSPNAG00000021526.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159522"/>
    <dbReference type="OMA" id="NFCQSGA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003309">
        <property type="term" value="P:type B pancreatic cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000176">
        <property type="entry name" value="pancreas/duodenum homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF11">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="144"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="146"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="312" mass="34766" checksum="036F344348272FD9" modified="2018-12-05" version="1">MQTLSHQEGNTDYSSVSKTLLKPLHLNSVVQHCSPCVLQEGSSAMEPGHHAGHVWDPLFLQKSHTPCAGFAQNTTRIGPHHTEQVNSLVKMTGTSARNVFRKTTYPWMSERQSTPSHGAANFCQSGASDEACSPERVRENDSRSARKRARSAFTSSQLLELEKEFHFSAYLCRPRRLEMASLLKLTDRQIKIWFQNRRMKYKKDHKGKATAWPSYSCQAVGASSSAEACAEMDVKAQDHLQFLNCSQSSYGQPTYKDWFLLAKAAPAITLDFSSAPQLASRPNDSPLASVSDLYLHQQEEGCLLVPPAPSYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CU40</accession>
    <name>A0A3B4CU40_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017567886.1">
        <property type="nucleotide sequence ID" value="XM_017712397.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CU40"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022088.2">
        <property type="protein sequence ID" value="ENSPNAP00000014261.1"/>
        <property type="gene ID" value="ENSPNAG00000020156.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436118"/>
    <dbReference type="GeneTree" id="ENSGT00940000162676"/>
    <dbReference type="OMA" id="HETGDTH"/>
    <dbReference type="OrthoDB" id="6370831at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017379996" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="146"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="363" mass="40509" checksum="63F9A84E034437D0" modified="2018-12-05" version="1">MITVILTFMLSLGPGITAYVSVIQSETLKTFIPGNDINLHCKIEEFHENYFSWFKQSLGQAPTCILTLYADSSPVRHGDFTHDERFTAEKKGTRFTLTIRNIREADSGIYYCAARDYDLVFFSNGVFLKYEGPNIKSFNYQINKIPSMDQTEDSPKLKEAVYPGDSVTLQCSVLTESCSGDHSVYWFRHGSGESHPGIIYTHGNRSDECEKSSETDSPTQSCVYKLPKRNLSLSDAGTYYCAVAACGQILFGNGSRLEITDPNSETAQMYWNLIALAASNVLCVIIIVVLLCARTKKRNISTSGQPERLHSTSAPFPSAVNEADERKELSYAAIHFPARKSKRRETQEETCTNVIYSSVHGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWT2</accession>
    <name>A0A3B4CWT2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zona pellucida glycoprotein 3d tandem duplicate 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWT2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023045.2">
        <property type="protein sequence ID" value="ENSPNAP00000014994.2"/>
        <property type="gene ID" value="ENSPNAG00000020968.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234567"/>
    <dbReference type="OMA" id="HFSPARG"/>
    <dbReference type="OrthoDB" id="8902383at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF26">
        <property type="entry name" value="ZONA PELLUCIDA GLYCOPROTEIN 3D TANDEM DUPLICATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043725025" evidence="2">
        <location>
            <begin position="22"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="3">
        <location>
            <begin position="107"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014994.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="378" mass="42325" checksum="BDF9B9AC1C53D970" modified="2024-11-27" version="2">MGVILLSMCILLGLLSPCARAEEGSSVWAELVLPELRVNQNQLGPPYLHLPVFLHTRVPLLDKAHFSPARGSGLEKLPERMRNVLVPPPRPTRPSDAENGRNRLRVACSATQMRVKIPRETVGSAEGLRLGTCDVSWSTKQHLVFLHYLHQCGIKREVINDRVVYSSMLHYTPLEALGSERPLPFSIPVQCHFNRFHYSYKIGFVPQVETLSDSCGCLCCSVSAQWSRLNSSEGYVVGQPMYFELDVYSVEEGERLFVNSCHVTMNNSRLSTPRFSIIDNYGCMVDSRSSSESRFLKSSRRNVVRFSVEAFVFQGKLAKQLYMHCETTVSSVTPTATSKFCTYNQKSGRWEELYGSNALCSCCNSTCLPGAPTGEECK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CXS5</accession>
    <name>A0A3B4CXS5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017553955.1">
        <property type="nucleotide sequence ID" value="XM_017698466.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CXS5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015586"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023813.2">
        <property type="protein sequence ID" value="ENSPNAP00000015586.1"/>
        <property type="gene ID" value="ENSPNAG00000021640.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427885"/>
    <dbReference type="CTD" id="569092"/>
    <dbReference type="GeneTree" id="ENSGT00940000155946"/>
    <dbReference type="OMA" id="HTSEMGR"/>
    <dbReference type="OrthoDB" id="7735550at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017298312" description="Fibrinogen C-terminal domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="10">
        <location>
            <begin position="302"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="223"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="267"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="526" mass="59996" checksum="732E2F9C7B70D9AF" modified="2018-12-05" version="1">MPALIMKFSLSVLLTLTITKPDLTVSGEVQEFDSRDEVLEREFLYAGRSKRSADPAGSPPPDKCSYTFIVPQQKTTGAICVNSKEPEALLENRVNKQELELLNGELHKQRRQIESLQQLVEVDGGVVNEVKLLRKESRNMNARVTQLYMQLLHEIIRKRDNALEVSQLENRVLNHTAEMQRLASRYRDLEHKYQHLASLANNQTVLIAQLEEQCLRGGFRGAGGGGGVARPARPYQPPVAPPQQQQPQLRLPHQPPAKPFHPPPFTRHNQITNEIQSDQNSKALPPPLPTMPAGTHSPSTTDKLSGPFKDCLQALEEGHTNSGMYLVKSETGNKLMQVWCDQSHDPGGWTVIQRRQDGSVNFFRNWETYKQGFGNIDGEYWLGLENIYWLTNQGNYKLLVTLEDWSGRKTFAEYASFRVEPEADFYRLRVGRYHGNAGDSLTWHNGKQFTTLDRDHDVYTGNCAHYQKGGWWYNACAHSNLNGVWYRGGHYRSRYQDGVYWAEFRGGSYSLKKVTMMIRPNPNTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0H7</accession>
    <name>A0A3B4D0H7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Grh/CP2 DB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GRHL1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0H7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016344"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024842.2">
        <property type="protein sequence ID" value="ENSPNAP00000016344.2"/>
        <property type="gene ID" value="ENSPNAG00000022549.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157612"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF16">
        <property type="entry name" value="GRAINYHEAD-LIKE PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
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    </dbReference>
    <dbReference type="Pfam" id="PF25416">
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    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="7">
        <location>
            <begin position="247"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016344.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="620" mass="70476" checksum="3F5985FC29AFDA2B" modified="2024-11-27" version="2">TEQEQELKRAVLVLQNDPAYNQRRAFTSEDEAWKSFLENPLTAATKAMMSINGDEDSAAALGLLYDYYKVPREKRTISQPKADILGSDVDPNKSRNLMAQLPETSMTSTDNRIQVLKGVVLPGNLVLPGNQLAAQDKKALFPSPDTTVTVSIAAVPNYPVKTEGPSHGFSITVPNPHCSEPDNHAVVFDRQLTHNQFSPNTQPRTPDSTFPETFKDGTQEVFSFPGDLQLRMASMAQEEYATFDTVSGNNFEYTLEASKSLRQKSGDGTMTYLNKGQFYPITLKETDNSKVLHHPICKVRSVVMVVFGEEKCRDDQLKHWKYWHSRQHTAKQRCIDIADYKESFNTISNIEEIAYNAISFTWDINEEAKIFISVNCLSTDFSSQKGVKGLPLNLQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQSRRKVKCPEVNTVFLGVFGWNVDVKVPLLHKRSDITTFKMMTELETQPVLFIPDIHFANFQRHVSISNFLFSGMKRLPYSEEEFGSPPSKLARVDEPKRVLLYVRKESEEVFDALMLKTPTLKGLVEAVSSSLLVSEHRLTHHYAATKILVNMDDNIIKHYSNEDTFQIHMEEMGGMVKLTLTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D498</accession>
    <name>A0A3B4D498_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">IL6R</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D498"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017921"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026924.2">
        <property type="protein sequence ID" value="ENSPNAP00000017921.1"/>
        <property type="gene ID" value="ENSPNAG00000024241.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165521"/>
    <dbReference type="OMA" id="QVIWISA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003530">
        <property type="entry name" value="Hematopoietin_rcpt_L_F3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015321">
        <property type="entry name" value="TypeI_recpt_CBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF22">
        <property type="entry name" value="CYTOKINE RECEPTOR COMMON SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09240">
        <property type="entry name" value="IL6Ra-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01354">
        <property type="entry name" value="HEMATOPO_REC_L_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017255846" description="Fibronectin type-III domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="499"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="359"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="593" mass="66356" checksum="D263DCBA40480CBC" modified="2018-12-05" version="1">MRLQSALLLLSGLFAVKVQCVQEPEICPRKEPPPGVLALTMGSEVILGCTGDITVDGVPLEMDTKQKERLRRRGGKTGLWTYQREGGNTGTTGTSHSTIRNTAVTGMYQNKTATGTYAETKGEPVTTVKTPTITTEEQTRNGRDLAVSHSTQPNRVGRVAEEGGAFSVTMEMGMSSERGTSMEYEDYEDYEDKEEGLRVTRGIKRQTRWTRNGQKVRGVKRGGVLRLPALRPTDSGNYSCYRGGQLISTVQISVGVPPETPTLSCRKKFHTSKLRCEWISRKPIIPRPVCYLLIKTGLRDFSRVQCSYSAERSRCWCALSSDEGDKDTYVAKLCVTNTAGNTTSQPHPFTLQNIIKPDPPAKVEVKSADSRPHTLGISWSYPATWKRGYYSLQFEIRYRPSLAKYYQYVEIDVEHEALVWWIMDALPHTQYEVQLRAKDEFDGVWSEWTDPVYGHTWTAPKPTIVSDVDATLEPFWTVPEGSGSEGDSKEEAPEAPNIAWVYVLWVFGLCLLVTVIVIFAFLLRHRLRFMSKMDKPSYSPPCSCSSPPPAALNQPLMAPRQQSQHHFLPAGEEEGKGQPVLMVLILNVVFSCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4W5</accession>
    <name>A0A3B4D4W5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CD163L1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D4W5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027748.2">
        <property type="protein sequence ID" value="ENSPNAP00000018503.2"/>
        <property type="gene ID" value="ENSPNAG00000024935.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183145"/>
    <dbReference type="OMA" id="NLTQCGH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000003">
        <property type="entry name" value="Deleted in malignant brain tumors 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000009">
        <property type="entry name" value="WC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF22">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="32"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="137"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="242"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="347"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="432"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="537"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="665"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="770"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="853"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="57"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="70"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="162"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="175"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="267"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="280"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="457"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="470"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="562"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="575"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="690"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="703"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="734"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="878"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="891"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="922"/>
            <end position="932"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018503.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1015" mass="109642" checksum="4D3D49BA534F38A7" modified="2024-11-27" version="2">MFITISNNRHAHDIILDKDISLEFSYRWQSGIRLVKGKRSCCGRVEIQYNYQWGTVCDDNWDINDAAVVCRQMGCGRAVSAPHSAHFGQGSGSIWLDDVACSGTESYLTACSNNGFGTHNCGHGEDDGVVCSNGLNIRLVDGDGVCSGRVEVYYNGQWGTVCDDSWDDKDAAVVCREMGCGRADRATESAHFGQGHDPIWMDDVYCSGRESSITACRHNGWGKHNCKHNEDAGVVCSGPDEIRLVNGKSRCCGRVEIKHNGWWGTVCDDDWDINDAAVVCRQMGCGKAVSAHSDAHFGQGSEPTWLDDVGCRGTENYLTQCSHRGFGKEDCGHQEDAGVVCSSNLNIRLVNPNGVCSGRVEIYHNGQWGTVCDDNWDMNDAAVVCREIGCGRAVSAHQSAHFGQGSDNWHHRFSLTDRDIYVLNNKSGPDEIRLVNGESRCCGRVEIQHNGQWGTVCDDDWDINDAEVVCREMGCGKAVSAPHSAHFGQGSEPILLDNVGCSGTESNLTQCSHRGFGTHNCGHQEDAGVVCSNSLNIRLVNTYGVCSGRVEIYHNGQWGTVCDDKWDMDDAAVVCRKMGCGTAVSAPHSAHFGQGSGPILLDDVGCSGNESSITACSHKGFGEHDCSHGEDAGVTCSGKLIFINVLSYIFFLQTMPNACIKSLQLQLVNGESRCCGRVEIQHNGQWGTVCDDDWDINDAEVVCRQMGCGKAVSAHRSAHFGEGSEPTWLDDVGCSGTESYLTQCSHGGFGKENCGHEEDAGVVCSKGFNIRLVSSDAVCSGRVEVYHNGQWGTVCDDGWDMNDAAVVCREMRCGRAVSAYGSAHFGQGSGPILLDDVDCSGSESSITAWQSGIRLVKGKRSCCGRVEIQYNYQWGTVCDDNWDINDAAVVCRQMGCGRAVSAPHSAHFGQGSGSIWLDDVACSGTESYLTACSNNGFGTHNCGHGEDAGVVCDYSYQRSSSSTSSRSSSIDITVGECTELITSHFITVILDVSLWQTHLGQTLHEFNINDLFQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBA8</accession>
    <name>A0A3B4DBA8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBA8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020366"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030872.2">
        <property type="protein sequence ID" value="ENSPNAP00000020366.2"/>
        <property type="gene ID" value="ENSPNAG00000027182.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234537"/>
    <dbReference type="OMA" id="WHELKEC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020366.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="210" mass="23840" checksum="B881C5FAD31559AF" modified="2024-11-27" version="2">MITRRVSRNPRTTQGDLVNDLQKAGTNLTKATVSNTLRRQGLTSCSARRVPLLKPVHIRARLKFAREHLDVLEEYWENVIWSDETKVVLFGTNTTRRVWRRVNAELRPKNTIPTVKHGGGNIMLWGCFSPSCSSSKGIAELVRVYEIMNGAMYCEILGANLLPSARALKMKCGWVFQHDNDPKHTARATKEWLRKKHSKVLDGRTCTIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DE52</accession>
    <name>A0A3B4DE52_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IRG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DE52"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040436.2">
        <property type="protein sequence ID" value="ENSPNAP00000021224.2"/>
        <property type="gene ID" value="ENSPNAG00000028650.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183007"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF10">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="71"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021224.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="356" mass="39647" checksum="0FBD8E5826B2BA91" modified="2024-11-27" version="2">MPREVSQASPPERRPWGRPRTRWRDYIAQLAWERLGIPLGELVEVAGEKEVWASLLRIKKAQEQIEHLPKVSLSVAVTGESGAGKSTFINAFRNLDDEDEGAAETGVTQTTTEPTPYEHPTVSNVVFWDLAGVGTPNFQAKTYDFFLNISSERFRGNDSMLAKEIQKKKKLFYFEFGNPKVFLISSLDVSEFDFNWLVATLLSELPQHKQCALLLLVPVSSVAILEEKVKMFEKEIWAAALLSAGIAVAPVPRSFFTRCYYAFCLDDKSLEKLSERVNKPDLTTLEKSPLLKELAQTSAVRMGVSALGQFLCSLIPVAGSAAAADISFTTTRDLLQKSLKELADEARKVLREAGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEW9</accession>
    <name>A0A3B4DEW9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ASB4</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548344.1">
        <property type="nucleotide sequence ID" value="XM_017692855.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEW9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022050"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033894.2">
        <property type="protein sequence ID" value="ENSPNAP00000022050.1"/>
        <property type="gene ID" value="ENSPNAG00000029289.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424675"/>
    <dbReference type="CTD" id="51666"/>
    <dbReference type="GeneTree" id="ENSGT00940000158874"/>
    <dbReference type="OMA" id="PIQYVLK"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03723">
        <property type="entry name" value="SOCS_ASB4_ASB18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="105"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="378"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="425" mass="47897" checksum="FB3F5ACAAFF895B8" modified="2018-12-05" version="1">MTEARASRRSEAVQQLKSRFLEALKRNDAAEVEKFLRTTNIDIDTVLDVKDTSMVLASYKQGYWLPGYKLESSWAMGIHVCMMYNALESALVLLQRGAAVNRKPNGKTPLHVACEVSHTDCVNLLLNWGAKVNSVSLSGHTPLHYCITQESVDCAKQLILRGANVNAESHNSDEDTPLHTAARFGISELVALYAAHGANVDAVNARMETPLITAAFWAMDTREQTYSEDHHLVCRMLLDYGANPNLQEEDKKTALHKASWNCDHVLIQVLLEGGANPSIMDVNGCAALQYVLKVMQFRPLSIPERCFQLLLNYGAVRVYPKQFHKVLQNCYDYPRAVEVMANSYEHLMHTKKWRAAIPDATYERHRTFYDSLFAVCTNNPRTLQHLARCTIRIAMHGCCETGIQQIPLPPPIRRYLLLEPVGIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFJ6</accession>
    <name>A0A3B4DFJ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Guanylate cyclase</fullName>
            <ecNumber evidence="3 14">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Photoreceptor outer segment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 14"/>
    <dbReference type="RefSeq" id="XP_017540159.1">
        <property type="nucleotide sequence ID" value="XM_017684670.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017540160.1">
        <property type="nucleotide sequence ID" value="XM_017684671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFJ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023182"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013286.2">
        <property type="protein sequence ID" value="ENSPNAP00000023182.1"/>
        <property type="gene ID" value="ENSPNAG00000007726.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412585"/>
    <dbReference type="CTD" id="140425"/>
    <dbReference type="GeneTree" id="ENSGT00940000165991"/>
    <dbReference type="OrthoDB" id="1890790at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06371">
        <property type="entry name" value="PBP1_sensory_GC_DEF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14043">
        <property type="entry name" value="PK_GC-2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000404">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000013">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000114">
        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF349">
        <property type="entry name" value="RETINAL GUANYLYL CYCLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12 14" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="550"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="19">
        <location>
            <begin position="889"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="540"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="826"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="540"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1123" mass="126437" checksum="A6112397CC2125B1" modified="2018-12-05" version="1">MQRQLCAFYSLPPEWAGSRHQKLNQRTFSHKYNHVSYCSTWPFWAYIVLLCFLLFPGSSVAIFRVGVVGPWSCDPLFAKAYPSTAARLAVSRINRDPSLWPGITFDYVILEEDCQTSQALSRFLSYYTRASGFIGPVNPGYCDAASLLGKSWNKAVFSWSCFSYELGNPRSHATFTHTMPLPALVLVRFMRHFHWAHVGIVASGDDVWIETAHELASALRSHGMPVGTVVSTGNDSVSMRKALAKVKKAEHLRLVILCMHSVLVGGTTQKLFLEMAHDMRMVDGSLVFVPYDTLLYSLPHHHLPQPILRNNSKLLQAYDAVLTITVESPEQHSFIHAFTEAQESGELPDSFQPHQVSPLFGTIYSSILLMAHAMRGVQAAGKWVSGGNLAQHAHNLTFKGFSHTVRTTQSGSALLDYVVLDTDGFTWELKPSYRIDMETDMVHFLGHSIHFPAWGPPKTDSSCWFTDGIICSGRMDVFQLIVISLSVFLLVISCIASAYFIRRRINQIRLVRGPDKILLTLEDVTFIIPSLSNKKLSFDDCRTSDTRKSFSERSKSPMSSQSPVTHENTNVAVYEGDWAWLKRLPDGNFRSINPSTSDVFELMKDMRHENVNPFMGFFHDCGVFAIVTEFCSRGSLEDLLLNDDVKLDWMFKSSLLLDLIKGMKYLHHKNICHGRLKSRNCVVDGRFVLKVTDYGYNEVLEAQKFPYVRPPAEELLWTAPEILRGSFPGLSGTLPGDVYSFSIVMQEVVVRGPPFCMLEQSAEEIIQKVMKPPPLCRPVVSVDHAPVECIQLMKQCWNEQPERRPTFEEIFDQFKNINKGKKTNIIDSMLRMLEQYSSNLEELIRERTEELEVEKQKTEKLLTQMLPPSVAEALMLGTTVEPEYFDSVTIYFSDIVGFTTISANSEPIEVVDLLNDLYTLFDAVIGNHDVYKVETIGDAYMVASGLPVPNGNRHAAEISNMALDILSAVGTFKMRHMPDVPVRIRIGIHTGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGMPYRIHVHSSTVKILLQLKAGYKVQLRGRTEMKGKGTEETYWLIGQDSFTKPLPIPPELKSGPEPKDFKLMLQKAVRKISAVRQVTQLTMADEGNVMIHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGP7</accession>
    <name>A0A3B4DGP7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Piwi-like protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the PET complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the argonaute family. Piwi subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541194.1">
        <property type="nucleotide sequence ID" value="XM_017685705.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017541195.1">
        <property type="nucleotide sequence ID" value="XM_017685706.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017541196.1">
        <property type="nucleotide sequence ID" value="XM_017685707.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017541197.1">
        <property type="nucleotide sequence ID" value="XM_017685708.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017541198.1">
        <property type="nucleotide sequence ID" value="XM_017685709.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGP7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022271"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034391.2">
        <property type="protein sequence ID" value="ENSPNAP00000022271.1"/>
        <property type="gene ID" value="ENSPNAG00000004662.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413269"/>
    <dbReference type="CTD" id="55124"/>
    <dbReference type="GeneTree" id="ENSGT00950000183200"/>
    <dbReference type="OMA" id="CILNTAN"/>
    <dbReference type="OrthoDB" id="445936at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034584">
        <property type="term" value="F:piRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02845">
        <property type="entry name" value="PAZ_piwi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000141">
        <property type="entry name" value="piwi-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000014">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000003">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23278">
        <property type="entry name" value="Piwi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="18">
        <location>
            <begin position="471"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="19">
        <location>
            <begin position="751"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="44"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="122"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1056" mass="117082" checksum="FCFC2A06ED3A9363" modified="2018-12-05" version="1">MDPSRPPGATRMPWLQPAGGRGFPADQPPVGRVRGLAVAAEELDAGRGRGRASETPALPYRRGIPLPATEPRSLPPVGFARGVPAPGEEQRLGRARGFLLSSLLPPVGIGRGLSLFVEPPAEPAPPVKRETVPLPAPEATPGPQEVKREEPPLPVQVDVPGPGSSLLSMMRGMGIEPAKTWGRGAVSVGRGSVGEFAPPVGSPSVAGEETAPEGVMLRGTSFVGRGLIGAGRTAMPPVGFGRGPTVSPALAPGPDAPSAIPVTPKDAPPVSADVPVTPGSSQAKAELKMEAACEPRKKAGTKGNPVVIGSNYIPIHCKNEAVFQYHVAFTPNVESLSMRFGMMRDHRSATGEVVAFDGSILYLPKRLEEVVHLKSERRTDNQEIDIKIQMTKILPPHSDLCIPFYNVVLRRVMKILGLKLVGRNHYDPNSAVVLGKHRLQVWPGYATCIKRTDGGLYLSVDVSHKVLRNDSVLDVMNVIYQQSRESFQDECTKEFIGAIVITRYNNRTYRVDDIEWSKSPKDAFTMADGSKMTFIDYYRKNYGITIKEVDQPMLVHRPKERSRPGGKQVITGEILLLPELSFMTGIPEKMRKDFRAMKDLTMHINVSAEQHTHSIKQLLQNISTNPEALHELSRWGLEISPEILVTQGRTLPMETICLQSASFISSPSVDWSREVVRDSSISTIPLYCWAVFYPRRAAEQAEELVAMFGKVAGPMGMRLDRPIRMELRDDRTETYVKSIHSQLTSEPNVQLVVCIITGNRDDLYSAIKKLCCVQSPCPSQAINVRTIAQPQKLRSIAQKILLQINCKVGGELWTVSVPLKHLMVIGVDVHHDTSKKSRSVMGFVASLNSLLTRWYSRVTFQMPHEEIINGFRVCLLAALQKYYEVNHSFPEKIVIYRDGVSDGQLKTVELYEIPQILKCFETFPNYEPKMTFIVVQKRINTTLYSYEGDRFGTPPPGTVLDHTVTNRDRVDFYLMAHSIRQGCGFPTHYISIYNTANLTPDHLQRLTFKMCHLYWNWPGTVRVPAPCKYAHKLAFLSGQYLHSEPAIQLAEKLFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLP9</accession>
    <name>A0A3B4DLP9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial</fullName>
            <shortName evidence="1">Glu-AdT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GATC</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (GATB) and C (GATC) subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017564781.1">
        <property type="nucleotide sequence ID" value="XM_017709292.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLP9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024385"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010253.2">
        <property type="protein sequence ID" value="ENSPNAP00000024385.1"/>
        <property type="gene ID" value="ENSPNAG00000001239.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434281"/>
    <dbReference type="CTD" id="283459"/>
    <dbReference type="GeneTree" id="ENSGT00390000018351"/>
    <dbReference type="OMA" id="RFAMLKH"/>
    <dbReference type="OrthoDB" id="5394539at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="223" mass="24648" checksum="6D5FCCAD19F87426" modified="2018-12-05" version="1">MFPCCFIRRSFLSSVVNVRSGASGLGATAPGSKVTAGRRLFTSQSLHRAAPLRPTQPRCRLSELPANGAWSTGRRAAGCRWSSNRSSSNADFSSKIPQFPTWEPVAESQLPPPTKVSLDLVDKLERLALVDFGNQEGVNCLEKAIRFADQLHVVNTDGVEPMDSVLEDRPLYLREDTVREGDCAEELLRLSKHIVEEYFVAPPGNIPLPTREERSAMLKHADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMJ5</accession>
    <name>A0A3B4DMJ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="10">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="10"/>
    <dbReference type="RefSeq" id="XP_017560187.1">
        <property type="nucleotide sequence ID" value="XM_017704698.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMJ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024331"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010391.2">
        <property type="protein sequence ID" value="ENSPNAP00000024331.2"/>
        <property type="gene ID" value="ENSPNAG00000001221.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431494"/>
    <dbReference type="CTD" id="55739"/>
    <dbReference type="GeneTree" id="ENSGT00390000000917"/>
    <dbReference type="OMA" id="QDDSGEC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000013">
        <property type="entry name" value="ATP-dependent (S)-NAD(P)H-hydrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000032">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 10" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="13">
        <location>
            <begin position="329"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="209"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="482"/>
            <end position="488"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="522"/>
            <end position="526"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024331.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="623" mass="68948" checksum="5F26B2CFA1CDA57F" modified="2024-11-27" version="2">MLVNPTRGFHWSDAETRALLNIWGEHDVQTALDGNFRNSHVYRDVAGRLGDMGYDRTPDQCRVRVKSLKRQYFQAKKASKKNSQCHKIFKFYEEMERILSSRSSATLDSQDIDSVAVEGDETMDGTEEDGESIDQPQDSDMEGTGECSNVEYPVKIEYPSYPIPVTVGSSSSIPAKQSSLQPNSMPSSSASRAKRFKKRFTNLSLEKLMEKFLEQSLEAEENFYRLEEQRLQAEDLRRETEHTRELHMLQMLGQMFAGLSRPVATQSPATQPTRNSHTGAPVPPTRLYFGNHSSQASEAEYANSSQQAAPVIERSFSLGRAAHRSMDSVLPLIKNTIPPLTSKKHKGQDGRIGVIGGCQEYTGAPYFAAISALKVGADLSHVFCTKDAATVIKSYSPELIVHPVLDSPNAVEEIEKWLPRLHSLVVGPGLGREDVILKTAKEVIEKSKSKGIPVIIDADGLWLVAKEPSIIQFYQRGILTPNFMEFSRLYEAMHREPLDSTDLKGSAQQLSIAMGNVTLVLKGEEDIITDGNRVITCSHQGSGRRCGGQGDLLSGALGVFAHWAFSSFPDATKGMNPTLVAAFAATSLTRQCNRQAFDKHGRATTTSDMIQEICTAFRKLFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DN57</accession>
    <name>A0A3B4DN57_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cell division cycle 40 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">PRP17 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CDC40</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017568494.1">
        <property type="nucleotide sequence ID" value="XM_017713005.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DN57"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024369"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010295.2">
        <property type="protein sequence ID" value="ENSPNAP00000024369.1"/>
        <property type="gene ID" value="ENSPNAG00000008910.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436483"/>
    <dbReference type="CTD" id="51362"/>
    <dbReference type="GeneTree" id="ENSGT00530000063583"/>
    <dbReference type="OMA" id="TLWHPHE"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000034">
        <property type="entry name" value="Pre-mRNA-processing factor 17, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="2 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="283"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="327"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="413"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="513"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="545"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="578" mass="65133" checksum="ACB5D11A50235EC6" modified="2018-12-05" version="1">MAAVVGSLVAYGSDSDSENEAESNTSPGKVDPDAVAHLQPLKSGRTTSLAVLDSAPEVAVKEDVETGTHLDPSLKEVNYNPTYETMFAPEFGPVNPFKSQQMAAPRNMLSGYAEPAHVNDFMFEQQRRTFSTFGYALDPSVDTTQVSSSSYIGAVEEAEKNKGLTVFEAGPKKAEKRKKVKGGDASEIDSFLGPWAKYQDEKGVAKPSEDEQKELDDIIAKRQKKGRNEDESPAEEKTILHVKDAYDYQGRSYLHIPQDVGINLRSADIPEKCYLPKKQLHVWTGHTKGVSAIRLFPASGHLLLSCSMDCKIKLWEVYNDRRCLRTFIGHSKAVRDICFNNSGTQFLSAAYDRYLKLWDSETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEVVQEYDRHLGAVNTITFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGAQNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVVSGDADGKLNIWDWKTTKLYHRIKAHDKVCISALWHPHETSKVITCGWDGQIKLWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DST5</accession>
    <name>A0A3B4DST5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574314.1">
        <property type="nucleotide sequence ID" value="XM_017718825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DST5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026515"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004935.2">
        <property type="protein sequence ID" value="ENSPNAP00000026515.1"/>
        <property type="gene ID" value="ENSPNAG00000011822.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440097"/>
    <dbReference type="CTD" id="503601"/>
    <dbReference type="GeneTree" id="ENSGT00390000018525"/>
    <dbReference type="OMA" id="CVREAIW"/>
    <dbReference type="OrthoDB" id="73614at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF1">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="166" mass="18563" checksum="1DB895AE70DE79C2" modified="2018-12-05" version="1">MDKLRAFVNGREGNNDNRNILQAANEASTLGWGTRVKGFIVCFAIGVGCSILGVCCLFIPKIGLILFIVFYTFGNICSLASTMFLMGPVKQLKRMCDKTRAFATVVMITCLVLTLCAVFWWKIFALSLLFVILQVIAFAWYGLSYIPFARDAVLKFFSMLCTMCMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVH8</accession>
    <name>A0A3B4DVH8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27429"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27430"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017570220.1">
        <property type="nucleotide sequence ID" value="XM_017714731.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DVH8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027076"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003479.2">
        <property type="protein sequence ID" value="ENSPNAP00000027076.1"/>
        <property type="gene ID" value="ENSPNAG00000012500.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437569"/>
    <dbReference type="CTD" id="560638"/>
    <dbReference type="GeneTree" id="ENSGT00940000157219"/>
    <dbReference type="OMA" id="XIKHESS"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031703">
        <property type="entry name" value="Lipin_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF10">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE LPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16876">
        <property type="entry name" value="Lipin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="7">
        <location>
            <begin position="719"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="159"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="340"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="604"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="633"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="648"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="936" mass="102386" checksum="D977273F22D4D78F" modified="2018-12-05" version="1">MEESGNERERRMVTSLECLEDFQDDPHDIDSSWSWSQTMNYVGQLAGQVFVQVKELYRGLNPATLSGCIDIIVVRQPDSSLQCSPFHVRFGKMGVLRSREKVVDIEVNGEPVNLHMKLGENGEAFFVTQTENDQEVVPSYLATSPILSDAAVLMGSSVQSLGPSLSGTESSAMKKRRKRRRKSKMDSVKREPSAEYSEDEEVFTIDISCNGGADHPADRNRDMINDDFSSVSSKQNVYARSDGDWSPMHSPNVSRPCSPKSDSELVTKAGEEKQQNPAMLWAWGELPQPSFLPSKPGSPAVCPVSIPVSHSTHFKVIPDASAATKHSRGDNTTSLRLFIPTPTTAEGTGSEHAMRMGSNHRGGGAENTTEGDEPAGVQNGVQGQDPSAARTNSETTAMLAARLVSEVEEGAVAAVGAVQQSKTDSPSKRKDKRSRHLGSDGVYLDDITELDPEVAALYFPKSESSGAGRVCCENTVRSVSMSPQSVGSSGADSGVDSYSEQTTDLPSIALSLCGGLTDSREITKEHFQEKAVSYSQFAANPALIDDPNLVVKIGSKYYNWSTAAPIMLSMQVFQMPLPKATIESIVKEKMPRKGGRWWFSWRGRGSSSKTDSASVNGAAGGAEHAGKHATISRRKDESSSSDEDHLSTKQTTSSSTQSESLLSQGGVSYRKTLRLTSEQLASLQLKEGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKIIISDIDGTITRSDTLGHILPTLGKDWTHQGIARLFHKVSQNGYKFMYCSARAIGMAGMTRGYLHWVNESGTMLPQGPVLLSPSSLFSALHREVIEKKPEKFKVECLTDIKNLFSPNTQPFYAAFGNRATDVYSYKEVGVPLNRIFTVNPKGELVQEHAKTNVSSYVRLGEVVDHVFPLLRRSSSSDFPCSDTYSHFNFWREQLPLVDSQECAGKISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXH6</accession>
    <name>A0A3B4DXH6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037400998.1">
        <property type="nucleotide sequence ID" value="XM_037545101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXH6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027699"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001875.2">
        <property type="protein sequence ID" value="ENSPNAP00000027699.1"/>
        <property type="gene ID" value="ENSPNAG00000013236.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438695"/>
    <dbReference type="GeneTree" id="ENSGT00980000198913"/>
    <dbReference type="OMA" id="GEKDQMN"/>
    <dbReference type="OrthoDB" id="8837635at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00098">
        <property type="entry name" value="IgC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017243401" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="45"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="149" mass="16891" checksum="D3929805E59266C5" modified="2018-12-05" version="1">MKVEALITAVLLLSLSCAELEEKMISFSRGVQLIVKAIKIRTVAPKVQILAPVGLQTPTSERVILTCVIRELQSNQISITWKINNTVVSQKHSSGEVFKQPDGTFTALGFYFVPLHDWRSDDVYRCEVKQEGVIYYEEVWHSLCDQYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E066</accession>
    <name>A0A3B4E066_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Janus kinase and microtubule-interacting protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">JAKMIP2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the JAKMIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E066"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028654"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015019.2">
        <property type="protein sequence ID" value="ENSPNAP00000028654.1"/>
        <property type="gene ID" value="ENSPNAG00000014515.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153713"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024836">
        <property type="entry name" value="JAKMIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031994">
        <property type="entry name" value="JAKMIP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935:SF7">
        <property type="entry name" value="JANUS KINASE AND MICROTUBULE-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16034">
        <property type="entry name" value="JAKMIP_CC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Janus kinase and microtubule-interacting protein C-terminal" evidence="5">
        <location>
            <begin position="409"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="206"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="281"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="389"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="471"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="667"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="864" mass="100883" checksum="EBC5E789879AB867" modified="2018-12-05" version="1">MAKKGRPKGEKPEALISALQAANEDLRSKLTDIQIELHQEKCKVNKLERDKVQESKRIREQEQHRHTAALTEQRARWHEEKLKELAALRESLTRQHEQELARTAKIKDGEIQRLRTALSALRDGSGEKVRTALTLEAREEARRFFDHERVKLVQEIAELKASKRQTDEALSTMIQADKMKAGDLRTEHQAHQEQISKIKWDCERDIRRLVDEIKAKDRTIFSLEKELETTTGFLQKLQLQKDALDEQLFLVKEAECNLGSPKREIPGRAGDGAEHCGSPDMRRNQRRIAELNSSIRKLEDRNALLVEERNELLKRVRETEKQCKPLLEKNKLLNKKNDELTQSLQRVEEKLKSLTKENLEMKEKISSHPPLKKLKSLNDLDQAQDDQEIEFLKLQVLEQQSMIDELTRDREKLLRKKRHKRSRPIKRHIVVDTFFGYDEESMDSETSSVASLRMDRTPATPDEDLDEGLAAEESELRFRQLTREYQALQRAYALLQEQKGGVLDAEMEAKAHEQLHADVQRYKAKIEDLEKELTLKGQDSKWVEEKQLFLRRNQELLEKVEKIDGENARLQQELQDSKDQNELLEFRILELEERERRSPPFTLTIHPFSEGVSALQIYCIKEGVKDVCIPDLIKLLDILGDNGNLRNEEQVAIIQASTVLSLAEKWIQQIEGTEAALHQKMMDLELEMEMFCKQKGYLEEELDYRKQALDQAYMQIQELEATLYNALQQDKVIKYGEPLSELQRDELRTAVEKLRRQMLRKSREYDCQVLQERMELLHQAQQRIRDLEDKTDIQRRQIKDLEEKALWQPRCLTSNCSLPLAKAKTVLVAIFCACMGGRGKCSAMVMDYRHLFFSLKLISNDVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0E2</accession>
    <name>A0A3B4E0E2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017568099.1">
        <property type="nucleotide sequence ID" value="XM_017712610.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0E2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017859.2">
        <property type="protein sequence ID" value="ENSPNAP00000030117.1"/>
        <property type="gene ID" value="ENSPNAG00000016650.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436239"/>
    <dbReference type="GeneTree" id="ENSGT00990000204023"/>
    <dbReference type="OMA" id="CPVFRAC"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="131"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="275" mass="31091" checksum="E40F7CB38FA1E4DB" modified="2018-12-05" version="1">MDKISTVFKKTQNVLREKLRNKRVGHRRDHITDTQQVEEKTGPTSDCLAQNGPGVHSQSPCGHIVTSSSLFNMVNQCLKKKNLDFSCPVCEEEWSWSQIRGQLQLSYAQTVILDTQVNRIIQDLPDVYKKCPVCLSILRRPMDSAGRPCPYSSCVHCPHTHHFCWSCLAPWFFPNAASSGQCSNQYCHVVATLLACETVTDAGSSVLGCPVFRACPQCYTLITPQPDCKFVVCPECFHRFCYICLDSETECVKREDPYQSPACKKPRAERQKFIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0G0</accession>
    <name>A0A3B4E0G0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">trypsin</fullName>
            <ecNumber evidence="13">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017551970.1">
        <property type="nucleotide sequence ID" value="XM_017696481.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0G0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028791"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015272.2">
        <property type="protein sequence ID" value="ENSPNAP00000028791.1"/>
        <property type="gene ID" value="ENSPNAG00000014603.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426761"/>
    <dbReference type="CTD" id="100008445"/>
    <dbReference type="GeneTree" id="ENSGT00940000164426"/>
    <dbReference type="OMA" id="WPWCYVQ"/>
    <dbReference type="OrthoDB" id="9406323at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031639">
        <property type="term" value="P:plasminogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033628">
        <property type="term" value="P:regulation of cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000001">
        <property type="entry name" value="Urokinase-type plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF38">
        <property type="entry name" value="UROKINASE-TYPE PLASMINOGEN ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 14" id="KW-0420">Kringle</keyword>
    <keyword evidence="11" id="KW-0617">Plasminogen activation</keyword>
    <keyword evidence="5 15" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017449009" description="trypsin" evidence="16">
        <location>
            <begin position="16"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="17">
        <location>
            <begin position="50"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="18">
        <location>
            <begin position="155"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="412" mass="46278" checksum="55CE1B7744751B01" modified="2018-12-05" version="1">MKQLVMAFLITCCFAASSTLRHKSWRENGILSVNDGDYAESAGCVPKGSDGSQYRGTVSVTARGNRCLRWSRIQSLDRIVASRGLGHHNYCRNPDNSLMPWCGVKRGTRIVREFCNIPQCETRPEPDQRPKGPEASYQDTETTCGEQSLNVLNKIIGGYRSPIESQPWVASIFRKKRGFDCGGTLIAPCWVLTAAHCFLYSQRKEDYTVYLGKNAINETDLSKEQMFTVTKLMIHERYESENYNNDIALLQIVNSKGQCAQRTSSVRTVCLPPAKQMMPYGAACKISGYGRVSLSSELKYSQYLKETYVNAISQIACDRDYYGKHEIPLTDNMFCAASPTWKEDACQGDSGGPLVYEQNGRMFLFGIISWGDQCATELKPGVYTKVTNYNKWIAKHTGLHSFTTGIMYPQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0G6</accession>
    <name>A0A3B4E0G6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1</fullName>
            <ecNumber evidence="6">2.4.1.122</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Core 1 O-glycan T-synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Core 1 beta1,3-galactosyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N-acetyl-alpha-D-galactosaminyl derivative + UDP-alpha-D-galactose = a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15621"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28257"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="EC" id="2.4.1.122"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.122" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0G6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029203"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035383.2">
        <property type="protein sequence ID" value="ENSPNAP00000029203.2"/>
        <property type="gene ID" value="ENSPNAG00000015077.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164651"/>
    <dbReference type="OMA" id="YHRERAD"/>
    <dbReference type="OrthoDB" id="414175at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016263">
        <property type="term" value="F:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000017">
        <property type="entry name" value="Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026050">
        <property type="entry name" value="C1GALT1/C1GALT1_chp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033">
        <property type="entry name" value="BETA1,3-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033:SF13">
        <property type="entry name" value="GLYCOPROTEIN-N-ACETYLGALACTOSAMINE 3-BETA-GALACTOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="24">
        <location>
            <begin position="67"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00041226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00059245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029203.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="319" mass="37173" checksum="101BB24752789806" modified="2024-11-27" version="2">MIPETLPPSLGPSSFKTYRSGEVSCDPTVVNMPLSQLFWNVLLGSNSKWANIFQRTIKTQVRLLCWVLTGPQFLESRAKHVHATWGKHCNKVLYMSSEESDFPTVKLNVTEGRDQLYWKTIRAFQFIYEHHLDDADWFLKADDDTFVVPENLRRLLSQLNTEDPLYLGRRFRPFIAQGYMSGGAGYVLSKEALRRFVKGHDDGSCTHFSDVEDMALGRCMETMKVTAVDTRDERRRQTFHAFPPDKYLLKQLTKSQPSHLLYEFYERVQGPGCCSDLTISFHYIRPVEMYMLEYFTYHLRPYGYKYRFNPEAALHKGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E139</accession>
    <name>A0A3B4E139_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynactin subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DCTN2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynactin subunit 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560699.1">
        <property type="nucleotide sequence ID" value="XM_017705210.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E139"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030342"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018310.2">
        <property type="protein sequence ID" value="ENSPNAP00000030342.1"/>
        <property type="gene ID" value="ENSPNAG00000017051.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431820"/>
    <dbReference type="CTD" id="10540"/>
    <dbReference type="GeneTree" id="ENSGT00390000003427"/>
    <dbReference type="OMA" id="YKFGDWE"/>
    <dbReference type="OrthoDB" id="4977at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15346">
        <property type="entry name" value="DYNACTIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0243">Dynein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="96"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="44386" checksum="54E7FF2667E9DEFE" modified="2018-12-05" version="1">MADPKYANLPGIAFNEPDVYETSDLPEDDQAQFESEELCSDSVERIVVNPNAAYDKFKDKRVGTKGLDFSDRISKTRRVGYESGEYELLAEGCGVKETPQQKYQRLVNEIQELTQEVEHIQANVKESSAEERLTPVVLAQRAAQLKQQLVSAHLDSLLGPNAHINLADPDGALAKRLMTQLELARGVRSSAGGEGKGSGLKGPDGVVLYELHSRPEHERFTESAKIAELGRRLADLETAVGSGTDKQGPLNAGLQGASVMETIELLEARVSALDAATLDQVEARLQSVLGKMNEIAKHKAAIEDAETQNKVSQLYDVVQKWDAMATSLPQVVQRLIAVKELHEQAMQFGQLLTHLDTTQQMINNALKDNSTLLTQVQQTMKENLVSVEENFAALEQRMKKLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1M1</accession>
    <name>A0A3B4E1M1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZNF516</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1M1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030517"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036193.2">
        <property type="protein sequence ID" value="ENSPNAP00000030517.2"/>
        <property type="gene ID" value="ENSPNAG00000017286.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161408"/>
    <dbReference type="OMA" id="ARMEKTH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000075">
        <property type="entry name" value="Putative zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000761">
        <property type="entry name" value="Zinc finger protein 449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051967">
        <property type="entry name" value="Krueppel_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN 516"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="912"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="48"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="173"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="430"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="575"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="660"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="694"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="767"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="822"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="852"/>
            <end position="865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030517.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="963" mass="106938" checksum="3D026B2F3D855FC4" modified="2024-11-27" version="2">MEVERQEITAGDPISKATHNKADEEKNQGHNCELCGRTFPFLSSLSQHMRKHTGEKPYKCPHCQHRSAQKGSLKAHIRSHKLDSMNQSPGVGDEEAEDEGEKEGGVPEEQGGCSSPTESTSACNKVVNGEEATKARKKGAKKERTSNEGGKQCSLCRKRLRSQAELEQHMQDLHDASQEEHPQARMEKTHPVEEESMIEEGPGVEEAEKENELVKGDFPCDQCDQVFTQAWFLKAHMKKHQSALDHGCRICGRRFREPWFLRSHMKTHGTKAKPKSDSEPPATVNNVSQDEASLVNEVCLYELCAKCGNFFHDRKSLRLHERVHNTEQPSNKSDDILSPATKRRFMECLNLRAAGEMEKLSEGSFGKRIPELDPVSSYQAWQLATRGRVVEVSEKSLGWEERLADADVAYDQEKGEYVLLRQEKRKKQLDASLSIPNKKKNSGQVSTGDGSAESLSDSEYRPSPRHSRKNSQNKTSECLECGKGFRTQQQMVIHMLIRHGGMSDSMNGVSLQESLFGKRQPSLSKPGDSATDVKDQRRHLVGKCRSTVESGTVAFRAFYHMAYSSVLIFADLRSTTPPSKNSDGRGGGKSKGQGEVSTEKVDASLLNLSVEADNSKEGEVQKSLVRHQCPYCSHATLYPEVLWIHQRIAHKVDSATLAPKWAPKNGLKGPKSILDFKRRTGPPPFLEGKDCPSLPQTRTFRTTPPESGPTRAKKPKPQNSSCESSSSQSKGGHTGGSAHTSKHKASNSQTDEIGTPKSRMEAQQHIATSTSTARPTASPQKTGSPKTGSRVMESSLLPPEGLRFMLSSKHNVSEQRSPKAQAPQTPSRSEPQVQDIQTSAGYDPWSRLGLPGSSSHSQPKSQQTAEGPEAFTDIFSFLKNCSPHDLAALYHHWGFSNIMMEQAGKRRERGEYVCPVCGKSFTQPSHYRTHMRSHTGKENTKAYTSRGVVYHSTKQDFPLRQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E266</accession>
    <name>A0A3B4E266_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ALDH16A1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017552963.1">
        <property type="nucleotide sequence ID" value="XM_017697474.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E266"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030707"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019001.2">
        <property type="protein sequence ID" value="ENSPNAP00000030707.1"/>
        <property type="gene ID" value="ENSPNAG00000017626.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427386"/>
    <dbReference type="CTD" id="126133"/>
    <dbReference type="GeneTree" id="ENSGT00940000161135"/>
    <dbReference type="OMA" id="MDYGPAP"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="1">
        <location>
            <begin position="537"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="795" mass="84676" checksum="5E8BCD6F4A6FD3A4" modified="2018-12-05" version="1">MANSTSKTVYDIFQSMEYGAYSPSTAATARAWLERHSYSLGFFMDGKFVCPVDRQTHTVTDSTGGTVCSVICAKDEDISSCGSSASAGFKTWSELSGHRRAKVLLRLASTLQRYTQCVSELCDLCQSPCSLPALIRLTQYYAGWAQLQDTLMPEWIPRGVVAVVVSDDCSFHSIWLKVLPALAMGNAVIMVPGVKLAPPTLLLAQLFMETALPAGVLSVVTGDTSVGARVAKNNQISYLTYSGSQQDGEVLAKQTAGWGLTISLCLSVSAECPFIIFESADIDSAVDGVIEAAFKKKKDWQWVLCVQESVLDSVVSRLKLRITGLKCVPLGSESDRTLVDVAVQDALQQGATLIQSCSPPASGALYPPTVLCGLAPPSSAAVSPPPGPLLPLLSFRSAAEGVALGNRTPHGQAASIWTEDLNLALETAKSLCVGSVWVNSHSVMDPSLPQCGRRDSGNCTDGGKEGLYQFLRPSPSPALPRSTPTSLDYAAFGTTASGFMVPEGFDPSSVTCSCSQYVGGKLCKADSGCSRTVQAPGGAVLAHCPDGGRKDVRNAVEAALKVQPGWLRKSPAARALSLYSLSDHLEKRRREMAASIQAQTGLSMEEADKEVELSISRLSDWAARCDKQSGGMPPLPQTGSALSAPEALGVVGVVLPDAKPLLSLVSLLGAAVSMGNAVILVPSEKYPLPAIDFIQVLQASDIPAGVVSVITGRRNQLTQALANHSEIQAIWYWGSLEGCQFLQHTCSSPLKRLWLHCEEVEGNDGGKYWSHSSPSLQEEMWREAVVWKSVWIPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4E7</accession>
    <name>A0A3B4E4E7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000018752.2">
        <property type="protein sequence ID" value="ENSPNAP00000030568.2"/>
        <property type="gene ID" value="ENSPNAG00000017559.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155638"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045995">
        <property type="term" value="P:regulation of embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000130">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009254">
        <property type="entry name" value="Laminin_aI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010307">
        <property type="entry name" value="Laminin_dom_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF47">
        <property type="entry name" value="LAMININ SUBUNIT ALPHA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06008">
        <property type="entry name" value="Laminin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06009">
        <property type="entry name" value="Laminin_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043837104" evidence="14">
        <location>
            <begin position="31"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="108"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="155"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="1196"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="1395"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="15">
        <location>
            <begin position="1572"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="240"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="410"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030568.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1762" mass="196069" checksum="42DC583F7D4AAE57" modified="2024-11-27" version="2">MSARRVRIGVLSSGALWLLIIWALLGLCVAGPRPFHRTPRHPTRQDWPGVQRVSFPSVDDREGQRIQFPSLNDVDTQKIEFPFVDAGQVTQRCARGFYRDRSSRCVPCACNGLSDECEESTGKCRNCQDNTAGDHCERCKDGYYGSAALRTCKICPCPLQLESNNFAVGCSEVAGRVQCLCKTGYTGERCERCASGYYDDPEFLEDRCRPCNCNGNSCSPKTGACNNGQDPKDTNTDEQCQECDNCAQTLLNDLDRLEDELSQIKAQLDSLNSSSSAYDRLKKLEAAIADTKNVVTDYSAAVDRLSPKVAELEKDALSVSEDIKLLLFKAQKRSNDAQTAAANIEKTHQRAKDLESQTLDLLRKILELLQQLKDAERGNGTVPSGDVATLMAEAERMVKEMQERSLKPQKDAAVKELDEAQKLLEYVKNNCTKQYDQNQEAAKRIKGLLSDYEAKLKELEEALKQASDTVMQANDQNSLNNKTLNDLLEKIKKLEKERDRVFADIVLAKRQLNDTENLLKMLSDSKKEYEQLAAQLDGAKKDLINKVNSISQAAAKEGLVKEAEDHAATLSRLANDLQEAVRNSSGRTDVQDALAAIDAYKNITDAIKAAEEAAKRAKDAADKALNDVVQGDVTNRAKDLKDEGNRLLKDATNAAKDLKQATDDLTNQQQRLDDAEKKKNALEKELAAINTDLKGVQRDDIGKMIDAAKKTAEAANTSATDTLDQLKAINKEVAKINATPSGSNIDRMLNDVDMTVKNLSSSIPSLLDKIAQVERLSSQIDPNNNISNNINRIKDLIDQARAAANRIAVPMKFIGDGYVELRPPKDLDDLRAYAALSLLLQRPNYNARGDGRRRRRQTNTDNGNLFVLYLGNKDSSKDYLGMALRNNVLYVIYKLNGRSYELETDSITLSGKEPFYFDRVDMHRIYEDVQIKLTKVFTSTNPPNPLIYTRRGEPLLNLLNLDPNEVVFYVGGYPDNFTLPPSLNYPKYRGCIEFSTLNEKFISLYNFKSLTPQNISDREPCKRYVIPRNDYFEGSGYAKVELVNMQSTLMISQWMESKADSAVLLYFTNEASFYCLTIEKGRMVMRGRDGDTILKEEISVGRFDLNGELKVIFQSSDKKIRVRMKSVEVLVFPYTLKKFDSYYIGGIPKDLRERDNITVLPLKGCVHSLSAAGTNPKVLERVGVGRGCYPELLTARKAEFNVGGSLETAPTGFDLSKDMVLSVGFRSTEKNGLLLQNKQDVNELELDMVDGHVMLTFQNNVWKSKKQYQDGEWHYLSVVKTGQNIEFRVDEDDDGEKNTVSPPALTFISNDIVLGKGTFTGCLSNLYMRRPDFDFKAEDLSTFSPYGDVLLDVCSAARPPENMLAGRNQTLNQDGREDAKGDEGSNCFTPTPIPHSFHLAGASSSLSYAVASTTLTNMPYFSLDIKTRSAEGLLFYIPAEQDNYHVALYVSKGRIRFSIGSQREILNRDKYNDGKWHTVTLSLEKRKFRLVIDGLRAKDGELNPREGSSIQLSSAIYLGTPPPSTHKVLKGKRLPEHGVIGCIRSFKMNGSLMAAAAVNRGVGPCFEGQTESGAYFSGHGAYVIVDESFVVGQSFELVFELRPSKQSALLLHVGNQNHLTVFMRREEVVAQVNSGGGEFSVVVQPKQSLCDGVFHRIAVIQRNNVVEMHVDTEGNYTIGPSFSSPTQDTHPVYVGGVPDHLKFSLPVTESYVGCLQNLVINRNVVVFEKLSGVFGAVNLRECPVNLRPPQHSPSAKSPVRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4K4</accession>
    <name>A0A3B4E4K4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">KPNA7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4K4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030685"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036283.2">
        <property type="protein sequence ID" value="ENSPNAP00000030685.2"/>
        <property type="gene ID" value="ENSPNAG00000017566.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244891"/>
    <dbReference type="OMA" id="QNHENGI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000009">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="245"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030685.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="520" mass="57551" checksum="D6FE64013184C455" modified="2024-11-27" version="2">MPTENLSDKRMAKFKNKGKEPTKMRERRVAECVELRKAQKVESILKRRNISSQPDEEPLSPEYSSDNQQQMMSVTIQEIIANVNSDCPKKQTQGCQTARKLLSRERNPPLKEIVEAGLLSRFVEFLSRNDNPTLQFEAAWSLTNVASGTSWHTQQVVEHGAVPAFIALLASPMLNISEQAVWALGNIAGDGPSYRDALIDCNVIPALLARLTPDAPTGYLRNLTWTLSNLCRNKNPFPRFSAVQQMLPSIIQLLHHSDKSVLSDACWAISYLTDGPNERIDVVIKTGILPRLVELLGFEELAVVTPALRSIGNIVSGSDLQTQMAIDSGVLNALPKLMRHPKPSVQKEAAWAVSNIAAGPRQQIQQLITCGLLGPLVDLLKNADFKTQREAVWAVTNYTSGGTVEQVVQLVKCGGLEAILNLLTVKDAKTVLVILDAVNNIFLAAEKLGEVEKLCLLVEEMGGLDRIELLQNHENNAVYRAAQGLIEKYFSENGEECLKTEATETDFVFGPTEVQRRFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4W5</accession>
    <name>A0A3B4E4W5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small nuclear RNA activating complex, polypeptide 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017538875.1">
        <property type="nucleotide sequence ID" value="XM_017683386.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4W5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031657"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020840.2">
        <property type="protein sequence ID" value="ENSPNAP00000031657.2"/>
        <property type="gene ID" value="ENSPNAG00000019162.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411688"/>
    <dbReference type="CTD" id="393224"/>
    <dbReference type="GeneTree" id="ENSGT00390000018691"/>
    <dbReference type="OMA" id="RDDMQNV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019188">
        <property type="entry name" value="SNAPC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15131">
        <property type="entry name" value="SMALL NUCLEAR RNA ACTIVATING COMPLEX, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15131:SF3">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09808">
        <property type="entry name" value="SNAPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031657.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41626" checksum="F9E8377C8A778189" modified="2024-11-27" version="2">MEHYRRAVQKDCERILGTFQASDSVRYEEFSTIWRQMNFSSIFYGQMERSETRAFARLALSVVSPYLFPPYTFQIRVGGLYLLYGLYNTQFTAPKEKIRLALKDWGELIRFQQDAVNAQHYDVVYILRKLLSEKAFYFTAMPDILFFNLKRKRGEKGRKLCDAFVDRPSRPQELISADMLEELANVHEHYEKLKQAVSPLPDTSGLSLIKQNLVPKLHSAVLSYYSWQQNQAEFDSQGGKSDVGAGEGTSCQESSQRAQLLQSIKSRSYGQAAEASKSRRHRQVELTSAATETTTGEVSNYKKVLSLKERTELRFMSHGNLNKELMGVTRLWCLTTLGEKTEEKKKRRFNWESEQESDEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E6C6</accession>
    <name>A0A3B4E6C6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E1 ubiquitin-activating enzyme</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin-activating enzyme E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UBA1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4E6C6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030866"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019247.2">
        <property type="protein sequence ID" value="ENSPNAP00000030866.2"/>
        <property type="gene ID" value="ENSPNAG00000017767.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165405"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.180:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000002">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF195">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="13">
        <location>
            <begin position="936"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="17"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030866.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1071" mass="119602" checksum="0480C7E09821566A" modified="2024-11-27" version="1">MAPNFWNDLPMLLRSMSSSPLSKKRRVTGSETKTGSHCSSSNSVRTELPHTPANGMAKNGNDAEIDEGLYSRQYVLGHDAMKRMQSSNVLISGLRGLGVEIAKNVILGGVKSVTLHDQGVAEWQDLSSQFYLREEDLGKNRAEVSQGRLAELNSYVPVTAYTGALTDDYLTQFQVVVLTNSTFDEQTHIGDFCHSKGIKLIIADTRGLFGQLFCDFGEEMMVHDTNGEQPLSAMISMITKDSAGVVTCLDEARHGFETGDYVTFTEVQGMTDLNGCQPIEIKVLGPYTFSICDTTSFSDYVRGGIVTQVKMPKKVAFKSFSSSISEPEFLLTDFAKFDRPGQLHVGFQALHSFEKKHSRRPKPWSQSDADELVALAGEVNAVQSGSAKQESLDQALLKKLSYLAAGDLAPVNAFIGGLAAQEVMKACTGKFMPIMQWLYFDALECLPEAEESVFTEEDCAPRSCRYDGQIAVFGSKMQELLAKQRYFLVGAGAIGCELLKNFAMMGLASGEGEVIVTDMDTIEKSNLNRQFLFRPWDVTKMKSETAAAAVKQMNPSVRITGHQNRVGPDTEKIYDDDFFESLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENAMQYLTDPKFMERTLKLPGAQPLEVLEAVYKSLVTDCPRSWEDCVAWARNHWQCQYNNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLEFSTSNDLHMDYIMAGANLFAQTYGLPGSTDRAALAKLLQEIKVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKTLLPPVESASQFKLCPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELLKIIQGHKKLESYKNGFMNLALPFFAFSEPIAAPKHKYYEIDWTLWDRFEVKGVQPSGEEMTLRQFLDYFKNEHKLEITMLSQGVSMLYSFFMPAAKLRERLDLMTEIVTKVSKKKLGKHVKALVFELCCNDLTDEDVEVPYVRYTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E757</accession>
    <name>A0A3B4E757_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">OB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E757"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031600"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020710.2">
        <property type="protein sequence ID" value="ENSPNAP00000031600.2"/>
        <property type="gene ID" value="ENSPNAG00000019037.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010045"/>
    <dbReference type="OMA" id="TFHFIDC"/>
    <dbReference type="OrthoDB" id="25571at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04478">
        <property type="entry name" value="RPA2_DBD_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031600.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26776" checksum="11D00CF2AB9ED14F" modified="2024-11-27" version="2">MHFIYQETSYMRFCSENCDEQTRSCINVSTQVGVVGIVRRTKPAVAYVVYSLDDMTGPSLDVKQWMDLEAANMNDRVIPPGTYIKVVGSLRSFENHRSMVAVSVRRVEDYNEITSHMMEVVQANLLNDGSSCSVVSVTSGVECWPFASKAKSLTVTTIMSLSSSNKSTSYGFTANQSQVGQNPQSPQHDFRLVCVSFFVSFCSRTCLKFLINEGHIFSTVDDDHFKSTYILFNINDELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E804</accession>
    <name>A0A3B4E804_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lipoxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the lipoxygenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E804"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031813"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021144.2">
        <property type="protein sequence ID" value="ENSPNAP00000031813.2"/>
        <property type="gene ID" value="ENSPNAG00000019011.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155191"/>
    <dbReference type="OMA" id="PPVNITC"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000001">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoxygenase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031813.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="515" mass="58902" checksum="CBF6220D4C2493D4" modified="2024-11-27" version="2">MPHIVQVDSASGLPYEVRFSFTKEKEFQHSLLNTSLDLKLKNFHPTNTWNSMEELEDLQWNAPWTHEYIKKHWMTDTFFGYQFLNGSNPMMIKRCSKLPNNFPVTNDMVKSALPKESSLEKEMEKGNIFLCDYARLDGIVGNVIDGIQQYLAAPLCLLFSNPQGKLLPIAIQLKQKPEAQNPIFLPTDSKHDWLLAKLFVRNAEFNEHELNFHLLRTHLLAEVFTMATLRALPSVHPLFKLLFPHIRYTLQINIMARKQLMSAEGIFSTSCAIGLKGMITLMRRATASLTYSSLCLPDNIKERGLENIPHYYYRDDGLKLWNIIHKFVNGVVAHYYTDRDVQEDSELQTWLTEIVEHGFLKNDNSGIPKSFHTVAELVKFVTMLIFTVSAQHAAVNNGQLDFGAWMPNFPSTMKAPPPTTKGQATAESILAALPDVKHTLKLMSALHLLSKKSSDHYPFGYYPEELFVEAVPLQKMEQFRQDLKALEGEIEARNKNLELPYDYLNPKNVANSVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EBM2</accession>
    <name>A0A3B4EBM2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 82</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM82</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017564392.1">
        <property type="nucleotide sequence ID" value="XM_017708903.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EBM2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032704"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022886.2">
        <property type="protein sequence ID" value="ENSPNAP00000032704.1"/>
        <property type="gene ID" value="ENSPNAG00000020823.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433986"/>
    <dbReference type="CTD" id="388595"/>
    <dbReference type="GeneTree" id="ENSGT00500000045021"/>
    <dbReference type="OMA" id="HVCQLYE"/>
    <dbReference type="OrthoDB" id="9943056at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031648">
        <property type="entry name" value="TMEM82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35257">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35257:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15816">
        <property type="entry name" value="TMEM82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="37090" checksum="527B79762920E5F9" modified="2018-12-05" version="1">MFSFFSSFVPSLPTWFTLETNPLNCILQGLVGACGISVLCNLLRVYLFVEAQSIADSKDDIKKKSSHGGLIQKLQFWILTVILGFVGSRVASLVVLEFCLRAVSSRLTAPSDKLGDSLLQLLVQCQFSLGCAFNCSLHFLHEEAPQAWLSLLLAAGLSWFLASQCSRLWHHVKAMYPIHSTQRYCGVCIGLLTTGTSILPFLCSALILTFSVASIAAISSINQHFLSTTEALRFWTPLTICYTLLIIYMNDEQHRQPSGQALLNMVIVRLGGLLVLMLTVGRWADVLHILVCFIGEAACLLPAQDLLDSAFKDVADVSRRSVMKTENQRRQKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EBQ2</accession>
    <name>A0A3B4EBQ2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">UHRF1BP1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017548846.1">
        <property type="nucleotide sequence ID" value="XM_017693357.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000033128"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023716.2">
        <property type="protein sequence ID" value="ENSPNAP00000033128.1"/>
        <property type="gene ID" value="ENSPNAG00000021546.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424987"/>
    <dbReference type="CTD" id="54887"/>
    <dbReference type="GeneTree" id="ENSGT00600000084428"/>
    <dbReference type="OMA" id="HSLISCN"/>
    <dbReference type="OrthoDB" id="43807at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026728">
        <property type="entry name" value="BLTP3A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774:SF15">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774">
        <property type="entry name" value="CHOREIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24917">
        <property type="entry name" value="BLTP3A_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="401"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="720"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="834"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="999"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1057"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1272"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="834"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="848"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="868"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="886"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1081"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1280"/>
            <end position="1289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1340" mass="145949" checksum="2A6BC30967677039" modified="2018-12-05" version="1">MAGIIKKQILKHLSRFTKNLSPDKINLSTLKGEGQLSNLELDEEVLQNMLDLPTWLAVTRVYCNKAAIRIQWTKLKTSPICLFLDKVEVEMRTCEEPRPPNGPSPIAITAGQSEYGFAEKVVEGMSVIINSITIKVQARAFHASFELWQLQGYSLNPKWQKTDLRYTRITHPKRGEVLTFKEINWQSLRIEADAIESEDQDLGSTPLRLITNQGRIRIALKRRVKDCNVLASKLLFILDDLLWVLTDSQLKAVIHYAKSLSEAMEKSAQQRKSMAAESLQTAPPSPGPHSLWAEPPPAPVGTPSTLAQYFDLHDVKESSYHTFISRLDLHICNDSSSAEPDDPPVPGSQGAMQLTFRKLGCDYYPFHRPGDGCRHWECYCGAMDSRAQWAAKLLQEFQQRVDSSGFPGPPTDQQTPSTQSPARKTQGDGESIPKSSPDGGPASRTAPGAALKRLRSSCMVIRVDDLDIHQVSTGGRNSKRTQSLLSCNRKSLQLAENTPAIHLQFTEYYFPHSPGMPVPFSNLYGQLNGLQLCVDAPSLLWLVLFSRGLLRTLDQVKAFYHLQDSSKSEEHVDIRVDVTQQKLVVPLDSSILDHPDRPQTLCFTLPQAVLSNTRHCPHGSCSDLQGIFSSFSKRPFFQTSLSPTFPCDRNTFHPLPPEFLCPPPEKRPTRAQDIWCLSLSRAALFFEGARRGPKGKALPFVEPFALSMWMCHPNALNQSSGSDLDASRSPDKLSQSGTNHVFPGQDVQEVPGAPSAPVHILAQSITPVKVWLNHYQYVALLRMKDFLARLAGDLTRDPQDSQSLEKKRTHPPAVCVSVLLEALELWLLLPPAVGEPEEVGGSPEETDTQSLTDSELSPSHQPGEPALLQNSAIRENAAANTRETHDQDDEGDGEGEGSVEEACEAVEEVDDEASQEEPQHATLVTSPPLSPGLTRDTSTFSLEGELSNALNATKDVTKDALNASLDLTKGALSITKDAFSILSRGSAMTKLFSTQIKEQAARPDDPGLRLQPLKHSPSQNSCDSAILDSSAADDGLSVDSDTSENFIILLDSESGLESMRPNGTPVHSGSRVSPAAGTEGGSSADLSSSLSQSTEDLSQDSVSVLLLSLGGVTSVMELRGEDVVMATEAQSLTPKQLGNQRVMELLAGLTVVQGGSSSPASPGALPAVCMRVEAGPSAGRYCAAAEGAGFLEVCVQGCRAELLASTLNTIGPFLEDELTADPQPIRLRIYNTSLTLKDDGPRAYPTAPQPVPVAFYLDGVVLERLDDGRLTLRPTTSQSRDGDAGRQDRACCGPGGEGKAEKIESLQSQLSETRSALAAALSERERLLQEIHKYNPAFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EC92</accession>
    <name>A0A3B4EC92_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integrator complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">INTS5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017545610.1">
        <property type="nucleotide sequence ID" value="XM_017690121.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EC92"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034162"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025711.2">
        <property type="protein sequence ID" value="ENSPNAP00000034162.1"/>
        <property type="gene ID" value="ENSPNAG00000023251.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108418237"/>
    <dbReference type="CTD" id="80789"/>
    <dbReference type="GeneTree" id="ENSGT00390000008374"/>
    <dbReference type="OMA" id="KDFCVHS"/>
    <dbReference type="OrthoDB" id="69088at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040316">
        <property type="entry name" value="INTS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029444">
        <property type="entry name" value="INTS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029445">
        <property type="entry name" value="INTS5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31697">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31697:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14838">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14837">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="27"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 5 C-terminal" evidence="2">
        <location>
            <begin position="267"/>
            <end position="1023"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14837"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF14838"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1032" mass="111169" checksum="5084ACFF65E5A698" modified="2018-12-05" version="1">MSAMFEGNALSALQTTHCPPQSNYSPQELALEIKAFISGVDQTKGRKLSVRDHARCAVRLLRTVPACRGAVLEHLRGVYDEYVAAFFQDLEAESDSGSNSTTGGTNANVSNLEDVIKEIHAVLLEFIRLNPKAWAPLVSSWAVDLLGQLSSKHAGRRATPHSSSLNELLQLWMSCAATRSLMECYSQCLAAMLAWCPDACVDALLDTSVQHSPHFDWVVAHIGSAFPGTIISRVLACGLKDFYAHGYPSGDKSTQLQAIDKASRVPKIGSVVGILGHLASRHSDSIKRELLRMFQDSLSPPPQNAPSSSGAAGWENSPHLRRATVPFLLQLAALSPTLLGAVSAELVELLRPPVLLQLQAQFQGLPREELENMLGLAVHLISQSPAGGARVLRFLADTATPASVIISGPTPSPHDGVREACDRLLQMLLLHLHKLVYNRPEGDEPYSHTTSSIAMPRVIPFLEELQAHVGELCAETLRLERKRHLWLHQLLCLLSVYGGPSIATEALCQLLTQARNPEELALASQLHSPLSSSIPGLLPSAVARCVAQIHTQNLNSRELAQLLNNLATAVQSQEEDCKAGGSTAGSSLHSSMAAQVGAAVSSHLHDFCPLLLHGDTMVSRAAARLLSCSQLPRACSPAHLLLISRAAVAHFFIALRQRGEGLKIGGQSGGEAVGYSMRLLSRLAAYSSLMLKAVLQQLVEGALHKGNRELFGGQREGPGEDGAPLPTLGPDLGVSLLNINCKFGTTVNFSGSVWSVFHAGVIGKGLKPPHTPSQSDADKVNQNMQTLLAVIVQCCSSGGGVSCSNGSGTSSGNGRYNHAGETISDEPSPVNAEAAKVIAVALVENVCPDVANGELSWPPEEHAKTTVERDIHIRRCFEANPVLFHLLRVVAAGRPALCYCSAVLRGLLATLLAHWEASREPSVLDSPWHLQASCLLVSCMGEGQLLPPVLSNIHEVFPHLSPFEVRLLLLGVWEYMRGNSPMPQKFTFSAEKGVFFRDFSRDGDVARYIAPIHSVLHKNIDRLGHLCWRFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECE9</accession>
    <name>A0A3B4ECE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine rich repeat containing 18b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017569811.1">
        <property type="nucleotide sequence ID" value="XM_017714322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECE9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034217"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038410.2">
        <property type="protein sequence ID" value="ENSPNAP00000034217.1"/>
        <property type="gene ID" value="ENSPNAG00000023343.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437307"/>
    <dbReference type="CTD" id="568967"/>
    <dbReference type="GeneTree" id="ENSGT00940000156026"/>
    <dbReference type="OMA" id="DSQEDWR"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF42">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 18-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="28831" checksum="5A8B405ADE527171" modified="2018-12-05" version="1">MAKGKKKSSESKGRKITLKMAKNALKLTVDGKRRLDLSNMEITTFPKCILKLCDVDELDLSRNMLKKIPDIIDSFVNLRWLDLHSNQLEQLPEAIGRLPKLHSLNLCNNLLTTYGIPYELGLLRNLRSLNLGMNRIETLPPSLAALKELRHLGLFNNLLMGVPQWLHSLPNLQTLNVKSNPICSDEPQKLDPIRRVECLYLVREGCLCSECLQKCKEERGKVDSRISKGLVQRRSILAGLITPNSVAQDDKAVCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECT5</accession>
    <name>A0A3B4ECT5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558669.1">
        <property type="nucleotide sequence ID" value="XM_017703180.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECT5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033650"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024734.2">
        <property type="protein sequence ID" value="ENSPNAP00000033650.1"/>
        <property type="gene ID" value="ENSPNAG00000022456.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430596"/>
    <dbReference type="CTD" id="10552"/>
    <dbReference type="GeneTree" id="ENSGT00950000183183"/>
    <dbReference type="OMA" id="YVWEPSP"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000030">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF11">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="370" mass="41560" checksum="877E0A23EBCF19E1" modified="2018-12-05" version="1">MSLQQFLLEPITCHAWNRDRTQIAISPNNHEVHIYKKSGNQWVKAHELKEHNGHITGIDWAPKSDRIVTCGADRNAYVWSQKDGIWKPTLVILRINRAATFVKWSPLENKFAVGSGARLISVCYFESENDWWVSKHIKKPIRSTVLSLDWHPNNVLLAAGSCDFKCRVFSAYIKEVDEKPAPTPWGSKMPFGQVMAEFGGAGSGGWVHSVCFSASGNRLAWVSHDSTVTVVDPTKSSTPSQLKTEFLALLSVIFVSENNIVAAGHDCCPMLFSFDDGGTLTFISKLDIPKQSIQRNISAMERFRNMDKRATTEDRNSTLETLHQNSITQVSIYEGDKRDCRKFCTTGIDGAMTIWDFKTLESSIQGLRIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ED49</accession>
    <name>A0A3B4ED49_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Trace amine-associated receptor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4ED49"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033648"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024742.2">
        <property type="protein sequence ID" value="ENSPNAP00000033648.2"/>
        <property type="gene ID" value="ENSPNAG00000030937.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182934"/>
    <dbReference type="OMA" id="LEPFNWF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF415">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="54"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033648.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="319" mass="35858" checksum="15B92A08CE89D290" modified="2024-11-27" version="2">VSETSRKYAWNYIRNISLCYEHHPNSCQKFIYPLAMRVIVYYVIGVIILLTLFGNLLVITAITHFKQLHTPTNYLTLSLAVADLLVGGAVMPASMIRSVETCWYFGSLFCKMHSSFAIMLCTASIFNLAFISVERYYAVCHPLQYHSKITPLTTLFMITVCWSVAAVVGIIYPELNVQGVEAYSDVICKGACVLVLGPMTSLSVSLFSLYAPTIIMLSIYLKIYLVARRQSRLVRNALSHVNKSRGQRNVQKADSKATKTLYCYHKMFHLNCCHTSTIFVLQHNNNNKKNIIITSSSSSSICLYLGIHCSCKISNSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK40</accession>
    <name>A0A3B4EK40_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THAP domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540381.1">
        <property type="nucleotide sequence ID" value="XM_017684892.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK40"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035576"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028759.2">
        <property type="protein sequence ID" value="ENSPNAP00000035576.1"/>
        <property type="gene ID" value="ENSPNAG00000025663.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412733"/>
    <dbReference type="GeneTree" id="ENSGT00940000166194"/>
    <dbReference type="OMA" id="RTDGNPD"/>
    <dbReference type="OrthoDB" id="413122at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001935">
        <property type="term" value="P:endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF7">
        <property type="entry name" value="SI:DKEY-228B2.6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="231"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="116"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="119"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="443" mass="50783" checksum="E5979F7464FF05AD" modified="2018-12-05" version="1">MVFYCCAYNCKNNTKDKSLAFHSFPLKNPELLKKWLRNIRWKDWTPEPHSRICSSHFERRCYSLISGPRRRLHTWAVPTIFSFPDHLQNRKRKIDPRSRRALRTIDESVLDLPSKRSLHSSTVLSPEPQPSTSITVRQSRPSEESGSQNPAELGTPQEPIQTPQTPNSSQSPVEPDKRPSPSWTILGDESLDRSMTIPSFFHSGYCLPRSFHWDGDDELNIMPHVVSGITGQTKPRIIEVTERWDWLGLDIRGPFPITSHKHTHIMTLTDYYSKWVEVFPLTQNKIRDAAVCLAEVIFQLGFPLGVLSRLPKHLLLEINRELKKQLNVNTSSLVLHHRQTGYLDLVTESLLNELVVKLVEEYPRSWDIQLPVASLRLSCTQHPTTGKKPFTVMYSKSLPAFIAPRELPCSAPDIRLSSFIIPHIEASKRSNKEETPSALPDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IHH0</accession>
    <name>A0AAR2IHH0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DEUBAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ASXL1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Asx family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000041757.1">
        <property type="protein sequence ID" value="ENSPNAP00000038725.1"/>
        <property type="gene ID" value="ENSPNAG00000021381.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055578"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035517">
        <property type="term" value="C:PR-DUB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042975">
        <property type="term" value="F:peroxisome proliferator activated receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026905">
        <property type="entry name" value="ASX-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024811">
        <property type="entry name" value="ASX/ASX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028020">
        <property type="entry name" value="ASX_DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13578">
        <property type="entry name" value="ADDITIONAL SEX COMBS LIKE PROTEIN ASXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13578:SF19">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN ASXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13919">
        <property type="entry name" value="ASXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13922">
        <property type="entry name" value="PHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DEUBAD" evidence="11">
        <location>
            <begin position="193"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="38"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="334"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="408"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="901"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="972"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1113"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1213"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1301"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1426"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1500"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="334"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="462"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="529"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="579"/>
            <end position="590"/>
        </location>
    </feature>
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        <location>
            <begin position="662"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="681"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="739"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="764"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="817"/>
            <end position="829"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="857"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="925"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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            <end position="995"/>
        </location>
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        <location>
            <begin position="1122"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1141"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1302"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1318"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1366"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1440"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1514"/>
            <end position="1530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1658" mass="174582" checksum="8A527F822AD9C0B3" modified="2024-11-27" version="1">MLHSQVRGDRVKNSIFFKLPGRMSLFTLKKNALQWTKNPTASETSDPTGASAASTTGSVGAAEGVEQESCDSTETAAASGENDASVDETSSSASCSTEPQTRLSRSSQSGRQRKKAVMMPRVVLTPLKVNGEHVPSGAAGRRREGSRGGPGPTLRARSELGWKRPQHFKSMRGLRSGPMKRNRGGVEVDFETPGSILVNTNIRALINMRTFAAFPPHSQQQLLQLLPEVDRQVGPDGMARLSSSALNNEFFTHASQSWKERLAEGEFTHEMQVRFRQEMEKEKKVEAWKEKFFEEYHGQKSGLTREEALKLTMSDAGDVAEAVLGADTAAVATPKRRGVGRRRREGRIRRRSRADLRRRARRPLGKTAPAAVVEAEQTEDQAASDVTATAASPVPETAAVQGEIVLPADSEPEATAEPICPEAAPSPVPASTPAPTSTSSTCDEPEASTRLLPEVVEPAVASTSSPSSSSSSTSSSSSPSSSPSSTSDRQGAFATSSDSSSSSSSSSSSSSSSSSSSTAAVATDPLDDGASVITTGTAGTGASSRESSPAASPATASPAIQLKDQKRRPDESQAYTNFPEKRARLDERQSFRNTVDCVHSEKPLPTTEEPKVPPIRIQLSRIKPPWVKGPPTYQICPRIVPPNEGSRRSGTGARTLADIKARAQQARAQREAAAAVAATGDGAGPGGGGPGGGTGIPDRSSGRRSREHPGPVEPGGGGGGGGGGGRRGGARVDVEEQESPASSHSSGAQLQLSNIETTEKTQASTPQTIPSPSSVSSNPSVLPSESPKTLTPSPPETDSPASQDPVEEICGVEEVATCSSDKASEQTLDTPVPTPSEPESVSSHQETRGEEADSSDSRTVTPSYTPVPSEAVNLVPTSIPDSLPRFGAQGVDVNRTLAVSSQSWEGNQNQSELRSGPTGVIQHGSDVKGSKETHVTVRNGFVESSDERAVRERILQEHKAGEVEADGKYGASYETSLPCLPDTTREDDTGMHSDSTETASDFENETQEDEPVDWHRTMDHNGVHSQNTKSQNQPVIHTQSRITSCSLSPPQHQQPVIQAHVSNPSHSQTVIQARFPNGMPNHPVIQAQKQHTIRHAPEQNSSALTQAHVQSYLTQGERDQSRAQNLNENSNGGKPFIPAEDDSKPFSRGPADDAGLKNSAAVLAVKRIQSSVRPVSSVEANNPLVTQLLQGSLPLEKVLPQPHSISKLEINRLPGASSGLPPGRQPGGHLRNLAPRFRGPAETGGPTESVGPEFQHKAPSTRVSPGPGRTYGPSPPGTAQSRLACLFEEASSRGTVVQQFIPQQPGGTTPPGAVPVITSLPSTSSSSRHPMDMNSQSAPGALESAVIKEHTTPQPSRAETPERQTAGFQQHSNNLPQPMCRTTPDAPSPPHGDLCPSEVVPTVKISWRPSKIQQPQHQSYQQQQQLSHAATVKNEVSARPSCQQALTKNSQAPISGNGSVVVVTKKEPQSSMDSYISGGGGGGAMEGLLNMELSLARMAKKEQGKNPYARQTDSSASPVSSSSSSPSATSASSLPYHLYGKLPKLQQSGGGGVLSGSTGGSSSGFSYTANVSVVDSSGFSRSIADSVLQLRPRVGIGNTGGQGTTLSIQAFADTAAEEVALKCSCRLKAMIMCQGCGAFCHDDCIGPSKLCVSCLVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJ95</accession>
    <name>A0AAR2IJ95_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Rabphilin-3A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Exophilin-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Plays an essential role in docking and fusion steps of regulated exocytosis. At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release. In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Also plays a role in the exocytosis of arginine vasopressin hormone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Interacts with RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B. Interacts with RAB3A; this interaction recruits RPH3A to synaptic vesicules. Interacts (via C2B domain) with SNAP25. Interacts with deubiquitinating enzyme CAND1; this interaction results in the deubiquitination of RPH3A. Interacts with GRIN2A and DLG4; this ternary complex regulates NMDA receptor composition at postsynaptic membranes. Interacts with SNCA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJ95"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067904.1">
        <property type="protein sequence ID" value="ENSPNAP00000039800.1"/>
        <property type="gene ID" value="ENSPNAG00000030906.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157468"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098850">
        <property type="term" value="C:extrinsic component of synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017158">
        <property type="term" value="P:regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061669">
        <property type="term" value="P:spontaneous neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04035">
        <property type="entry name" value="C2A_Rabphilin_Doc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08384">
        <property type="entry name" value="C2B_Rabphilin_Doc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15762">
        <property type="entry name" value="FYVE_RP3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000032">
        <property type="entry name" value="Double c2-like domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000023">
        <property type="entry name" value="Double C2-like domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000182">
        <property type="entry name" value="rabphilin-3A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028698">
        <property type="entry name" value="FYVE_RPH3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043566">
        <property type="entry name" value="Rabphilin/DOC2/Noc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047022">
        <property type="entry name" value="Rabphilin_Doc2_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729:SF3">
        <property type="entry name" value="RABPHILIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 26" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RabBD" evidence="30">
        <location>
            <begin position="42"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="29">
        <location>
            <begin position="90"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="28">
        <location>
            <begin position="271"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="28">
        <location>
            <begin position="428"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="221"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="27">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00066191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00074062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00075517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="572" mass="64094" checksum="A055C4BD9C4EBEBC" modified="2024-11-27" version="1">HTSCNFSNAENAALFALMGGVSISKINSNMAQTELQTQCSGPGPGPELTDEEKEIINSVIARAEKMEAMEQERIGRLMNRLDNMKKTVCGDGLSRCLLCGEQLGSLGKSVVCEDCKKNMCTKCGVQNNSRPRSVWLCKICNEQREVWKRSGAWFFKGLPKQFLPSPMPISKSKEPNFQKAAENQATLDASVAAAQPQPQFGNTFLFAATALQEPERGAYLTTGFQDRVPPAEREERKQSASYNTPPARHQAPPPEEDEDEANSYDSDETTTLGALEFSLLYEQENNSLHCNIIRAKGLKPMDSNGLADPYVKLHLLPGASKSNKLRTKTLRNTRNPAWNETLVYHGLTDDDMQRKTLRISVCDEDKFGHNEFIGETRVALKKLKNNQKKNFNVCLERVVPTKRTATAGAARGIALYEDEVNDGGEVEERGRILISLMYNSQQSRLIVGVIRCVHLAAMDANGYSDPFVKICLKPDMGKKAKNKTQIKKKTLNPEFNEEFSYEIKHAELAKKTLDISVWDYDIGKCNDYIGGCQLGISAKGERLKHWYECLKNKDKKIERWHTLLNENHVSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJY5</accession>
    <name>A0AAR2IJY5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PIP5K1A</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJY5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062016.1">
        <property type="protein sequence ID" value="ENSPNAP00000039948.1"/>
        <property type="gene ID" value="ENSPNAG00000007665.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154703"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17301">
        <property type="entry name" value="PIPKc_PIP5KI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000001">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 5-kinase type-1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF54">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE TYPE-1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="5">
        <location>
            <begin position="59"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="476"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="485"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="619" mass="68554" checksum="893C59C88149F0EB" modified="2024-11-27" version="1">MATAGQVPTDGVNPTGTTGTGRMASLEGLGSPSSSQTNKKTIGHRGIDPTGETTYKKTTSSALKGAIQLGIAHTVGSLSQKPERDVLMQDFEVVESIFFPSEGSNLTPGHHHGDFRFKTYAPIAFRYFREMFGIRPDDYLYSLCNEPLIELSNSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYFMNLNQNKRTLLPKFYGLYCVQSGGKNIRIVVMNNLLPRAVPMHLKYDLKGSTYKRRASPKERNKSLPTYKDLDFIQDLPDGLLLENDHYNALCKTIQRDCLLLQSFKIMDYSLLMGVHSVDRAGEEASTAVHDHRRAQGQKALYCTAMEAIQGEAKGKTPPQSNESLGGIPARNSKGERLLIFIGIIDILQSYRLVKKLEHSWKALVHDGDTVSVHRPGFYADRFQKFMCNTVFRKMSLKTSPSKKIRAGVVSAVRKANTAVSGQSHQPQPLDAPSSANVIQTNAEQTHTDQDTTRLSNSSAVSLTSETEKPGDGDMQPRPDLLPRPLPHDDVTSNSADTTHSTFSLGSLEPAPSSPALRCVHVCVVIAFMCVTVSHCCRRLYFHFHCTFCYVFLAFQRGRAEAMQCRSEPVCSRKHCICPVVRGQSEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ILQ6</accession>
    <name>A0AAR2ILQ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2ILQ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048565.1">
        <property type="protein sequence ID" value="ENSPNAP00000038646.1"/>
        <property type="gene ID" value="ENSPNAG00000005641.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286927"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000782">
        <property type="entry name" value="Si:ch211-196h16.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001632">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="411"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1267" mass="141607" checksum="3EFA3057836D01B7" modified="2024-11-27" version="1">MEEADRQQDLKVVGAGKSESGNKVRQLIAKFEKQAAANAGRGYTNTDNEMEDLERRRKHQHLGGGRLTSKISGSGEHKTEELKSLRKRKRPDSPEPSCVSMKRDQSMGRGIDFKQGESSPELSHFQSERPDSPEPSCVSMKSDDSMGKGIYFKQGESSPELSHFQSERPDSPEPSCVSMKSDASMFRDTTFKQGESSPELSHFQSERPDSPEPSCVSMKSDQSMGRGIYFKQGESSPELSAQKERAKITMTHLESIFQELEHKVISLVKNQLKRFKKVLSPDFPACSEREVEGEVDQTVREGTLQIALHVLRNMNQTDLASTLQTKLVPTCHQKFKSTLKKKFQRINEGISYHGGSTLLNEIYTELYITEGGSGEVNNEHEVRQIETASRRPATQERPINCSDLFKDTAIRTVLTNGVAGIGKTVSVQKFILDWAEGKANQDILFIISLPFRELNSVKSRKLSLVDLLRLFFTDIKQLKPADYHQYKVLFIFDGLDESRLPLDFQNNESLSDVTQSASVDVLLTNLIKGNLLPSALLWITSRPAACGQIPPECVDQVTEVRGFSDPQKEEYFRKRISDQSVANRVITHMKSSRSLYIMCHIPVFCWIAATVLERMLGEAESGEIPKTLTQMFTQFLIFQIKHKDQKYHGNCDTDPQQTREMILALGKLAFQQLEKGNLIFYEEDLRECGIDVRGVSVYSGVCTQIFREELGLHLGKVFSFVHLSVQEFLAALFVFFSFISQNRNVLNHQTHSKSTMTDFLKSAVDRALQSENGHLDLFLRFLLGLSLESNQTLLRGLLTQTGSTSNCREGTVEYIKEKLRENLSPEKYINLFYCLNELNDHSLVQEVQTYLNTGKYCRLSGFTLSPAQWSALVFVLLNSEEELDVFDLRKYDRSEECLLRLLPVVTASRKAVLSDCRLSERSCAALASVLRSNSSSLRELDLSGNSLCDSGVTLLSAGLEDQHCKLETLRLSGCRLSERSCAALASVLRSNSSSLRELDLSHNSLCDSGVTLLSAGLEDQHCKLETLRLSRCDFSERSCAALASVLRSNSSSLRELDLSYNSLCDSGVTLLSAGLEDQHCKLETLRLSGCRLSERSCAALASVLSSNSSSLRELDLSDNSLCDSGVTLLSAGLEDQHCKLETLRLSRCDLSERSCAALASVLRSNSSSLRELDLSDNSLCDSGVTLLSAGLEDQHCKLETLRLRDCSITEKGCKALVKALKLNPSHLRELNLEDNEPGDSGVKMLSALLEDPHYKLEELHIRRRSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IPN9</accession>
    <name>A0AAR2IPN9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Functions in replication-dependent translation of histone mRNAs which differ from other eukaryotic mRNAs in that they do not end with a poly-A tail but a stem-loop. May participate in circularizing those mRNAs specifically enhancing their translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with eif4g1, eif4g2 and slbp; probably tethered by SLBP to the 3'-end of mRNAs ending with the histone stem-loop, it also interacts with eif4g1 which is bound to their 5'-end.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIF4GD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577124.1">
        <property type="nucleotide sequence ID" value="XM_017721635.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392251.1">
        <property type="nucleotide sequence ID" value="XM_037536354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IPN9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087940.1">
        <property type="protein sequence ID" value="ENSPNAP00000039984.1"/>
        <property type="gene ID" value="ENSPNAG00000030163.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108441885"/>
    <dbReference type="CTD" id="393355"/>
    <dbReference type="GeneTree" id="ENSGT00940000153432"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051367">
        <property type="entry name" value="mRNA_TranslReg/HistoneTransl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254">
        <property type="entry name" value="EIF4G DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254:SF17">
        <property type="entry name" value="MIF4G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MIF4G" evidence="9">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="221" mass="25667" checksum="166B8D237569E7B4" modified="2024-11-27" version="1">MDHIWEECKIQAFDSETQNLIKAALKDSSAVDLEKLSEAIVDQSLKDRSLCRDAGHICYALVQTEAKHSNTNVFRRNLLTRLQKEFVEREETRKRSVQEWVCVVSFICNIFDYLKVNNTPMTALVDPVYDCLFRLSQPDALLNEEEVDCLALQLHRVGEQLEKMNAQRMDQLFYLLRDGFLLQDNLSSMTRLLLLEVLEFRASGWTLSAAAQRYYYSEVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ISH2</accession>
    <name>A0AAR2ISH2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ISH2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060494.1">
        <property type="protein sequence ID" value="ENSPNAP00000042673.1"/>
        <property type="gene ID" value="ENSPNAG00000037447.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267173"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000022">
        <property type="entry name" value="Cardiac titin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050958">
        <property type="entry name" value="Cell_Adh-Cytoskel_Orgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080">
        <property type="entry name" value="CONTACTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080:SF8">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="14"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="170"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="262"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="356"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="453"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="576" mass="62881" checksum="084591955F787F0F" modified="2024-11-27" version="1">NTTVRFKSTFKGTPPFTVKWFREDTELITGPSCFIGLEGLSCFIDLYAVGISNTGTYSCQVSNDAGTTSFFYVLLPPSSLLRALHRFQFVYFVVAFYSKVTGTPPLKISWYKNDATVTDTDNMRMTFDDSVAVLEIFSTSNNDDGVYTCEAQNDAGTKSCSTTLSVKEPPTFHKKPTPVEGLKGKDASLNCELKGTVPFEITWFKASKQLKESRKYKFVSEGHSATLHILGLEASDAGEYECKASNNVGSDACQATVKLREPPVFVKKLSNTTAVSGEEVTLVATVKGSKPISVSWVQDKDHILTDSDNRKIAFENNQVTLKIFKVDPTAAGKYTCQLKNDAGVAECMANLTVLEPAAILDKTESVSVTAGDAAALECSVSGTPELKPKWYKDGVELSSGRNYKITFSKRISSLKVLSAQKGDTGEYTFEIKNEVGSDSCKMHLTVLDKIIPPTFSRKMKDTQSIIGKHIEMDCKVSGSAPLTISWYHNGEEVTSGPNYEISFADNTCTLKLPNLKLSDSGSYTCKALNSAGAAETTLIFATLESLEGNWSSKLLLVWLVCLSLIYAQFSCEHCPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IT04</accession>
    <name>A0AAR2IT04_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ADCK5</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037391277.1">
        <property type="nucleotide sequence ID" value="XM_037535380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IT04"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068664.1">
        <property type="protein sequence ID" value="ENSPNAP00000040746.1"/>
        <property type="gene ID" value="ENSPNAG00000024279.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438864"/>
    <dbReference type="GeneTree" id="ENSGT00940000159821"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF28">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="171"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="580" mass="66358" checksum="AEC44BE8D101AD5C" modified="2024-11-27" version="1">MAGDPFRTLRLCRRHLALLQKCSSPINHQVLLRRPLSSHRAVRSRRLLRNLLIGASVGVPLVVGVRYAVSDPKDQRRMRILAEGVGRFCRSLYVGLLISVDYWWTSSIVLRGTDESSAEYAAAMSACHQRAASGMVVGAVQNGGLYIKLGQGLCAFNHLLPPEYIRTLQVLEDRALNRRYKEVDALFLEDFGSTPDKMFKTFDYEPVAAASLAQVHKAELHDGTPVAVKVQYIDLRDRFDGDIRTLEILLDVIKFMHPSFGFRWVLKDLKGTLAQELDFENEGRNSERCAEELKHFKFIVVPKVFWGLTSKRVLTAEYCEGCKINNVEEIKRQGLSLKDTADKLIRTFAEQIFYTGFIHADPHPGNVLVRRGPDKKAELVLLDHGLYEHLSKSDRVALCQLWRSIILRDEAGMKRYSNDLGVKDYFLFCEMLLQRPISMRELTLGNILSREETSYMRDMAVHHFDSIMQVLKSLPRPMLLVFRNINTVRCINITLGAPVDRYFVMAKCAVRGWGKTRADDAAVLPSLRILRWVWSFWENLKFECALRSEMAMMSLTRSLLRLLSYCGVITVDPDVYQYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITC8</accession>
    <name>A0AAR2ITC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ITC8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056829.1">
        <property type="protein sequence ID" value="ENSPNAP00000042970.1"/>
        <property type="gene ID" value="ENSPNAG00000030967.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286899"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="147"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21744" checksum="0998DECE5AEFB8D4" modified="2024-11-27" version="1">MASISVDQDQFSCPVCLDLLKDPVATPCGHSFCMVCINGCWDQEDQRGVYSCPQCRETFTPRPVLRRNNVIAEVVEKLKKTELQAGSPALCYAGPGDVECDFCTGRKHKAIKSCLVCVVSLCEAHLKPHLEIPALVKHKLVTASSKLQEKICPQHNKQIEIYCRSDQQFICYLCTMDEHMGHSTVAAVAERKETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITV4</accession>
    <name>A0AAR2ITV4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Copine-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Copine III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">RBM12</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Calcium-dependent phospholipid-binding protein that plays a role in ERBB2-mediated tumor cell migration in response to growth factor heregulin stimulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Monomer. Interacts with ERBB2 (preferentially with the tyrosine phosphorylated form); this interaction occurs at the cell membrane and is increased in a growth factor heregulin-dependent manner. Interacts with SHC1; this interaction may mediate the binding of CPNE3 with ERBB2. Interacts with RACK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2ITV4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059439.1">
        <property type="protein sequence ID" value="ENSPNAP00000043178.1"/>
        <property type="gene ID" value="ENSPNAG00000007429.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162210"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903265">
        <property type="term" value="P:positive regulation of tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12745">
        <property type="entry name" value="RRM1_RBM12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12512">
        <property type="entry name" value="RRM3_RBM12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000042">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000099">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000193">
        <property type="entry name" value="RNA-binding motif protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034591">
        <property type="entry name" value="RBM12_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034855">
        <property type="entry name" value="RBM12_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF2">
        <property type="entry name" value="COPINE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 20" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="23">
        <location>
            <begin position="303"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="23">
        <location>
            <begin position="435"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="22">
        <location>
            <begin position="588"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="89"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="377"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="104"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="401"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00058857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1096" mass="119516" checksum="F29AA6317D935A6D" modified="2024-11-27" version="1">MAVVIRLQGLPIVAGTMDIRHFFSGLTIPDGGVHIVGGEHGEAFIVFATDEDARLGMMRTGGSIKGSKVSLLLSSKTEMQNMIELSRRRFETGSAEGSAGNGNRSGPPTSTGGSGRGSLPTTAQGFSNTASTTATTAVSTNEPVNNKNAPAFASTTMGNMPANFNNFSSPSLSVGSTMTTAMSSLSSVPPPIPPLPTMPTLPPMPPMPVPPPVSTLPPVPAVNPLTSVPPVPPMTHMPHLSAIPPFNPGVPPPVGPVTGGLAASGPPLSLGNPNPMFLNPLNPLNPLSLQAHLKSAVGNPDELYVQLQGLPFSASEIEIRDFFHGLGVDSVRILRDNLGRSNGRALVKFFTPQDSFESLKRNSGMIGQRYVEISPATERQWRDSQGSSIGHDKLGNDSEHNRHHHHHHHHRGSASSSSPSGRERARSRSPHKQEFCVYLKGLPYEAENKQIFEFFKNLDIVEDSIYIAYGPNGRATGEGFVEFRNEMDYKAALGCHMQYMGSRFIQVHPISKKNMFEKIDTIRKRLQGSQGDQKGGSSEGGRGARTCAHITNIPYNVSKKDVRLFLDGIQLFEDSLKVLVDGNGNGLGQAVVQFKSEEDALKMANQCVTKIELSISCKNLLDKDVGSKSDPLCVLLQSVGDDKWTEVDRTEKVKNCQDPEFSKRLCIDYYFEKVQKLKLGVYDIDNKSVDLKDDDYLGGYECTLGQIVSSKKITNPLQLKIGKPAGKGTITVCVEMLDLFGKSDPFLEFFKQDDDGKWLLVHRTEVIKNNLNPSWKKLTVSLHTFCSGDLNKPIKVHCSDYDNDGSHDLIGVFQTNLNILLLYKNSGVVRIKYCKIEAQYTFLDYVMGGCQINFTVGIDFTGSNGDPRSPESLHYLSPNGVNQYLTAIWSVGQVIQDYDTDKLFPAFGFGAQVPPDFKVSHEFSLNFNPSSPYCQGIDGIVQAYRMALPQVRLYGPTNFSPIINHVARIAATAAQQQNAAQYFVLLIITDGEITDLDQTKQAIVNSSKLPMSIIIVGVGEADFKAMEVLDGDNGVLKSPTGEPVARDIVQFVPFKDFANATRESLAQTVLAEVPNQLVSYFKMRNLSPVNPPAPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IX21</accession>
    <name>A0AAR2IX21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein MON2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MON2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MON2 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000053113.1">
        <property type="protein sequence ID" value="ENSPNAP00000042597.1"/>
        <property type="gene ID" value="ENSPNAG00000024980.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013286"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF333">
        <property type="entry name" value="PROTEIN MON2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="9">
        <location>
            <begin position="8"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="7">
        <location>
            <begin position="212"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HDS" evidence="6">
        <location>
            <begin position="842"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="8">
        <location>
            <begin position="925"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="511"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="521"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1713" mass="188379" checksum="86181B1FD57B14BD" modified="2024-11-27" version="1">MSTTSPEAVKKLLENMQTDLRSLSMECKKKFPPVKEAAESGIVKIKTIAARNTDILAALKENSSEVVQPFLMGCGTKEPKITQLCLAAIQRLMSHEVVSEAAAGNIINMLWQLMENGLEELKLLQTVLVLLTTNTVVHDEVLSKAIVLCFRLHFTKDNITNNTAAATVRQVVTVVFERMVAEDEKFKGMAEQPPAVQGNSNRRSVSTLRPSAKDAYMLFQDLCQLVNADAPYWLVGMTEMTRTFGLELLESVLNDFPGVFLQHQEFSFLLKERVCPLVIKLFSPNIKFRQGSSSSAASPAPVEKPYFPICMRLLRVVSVLIKHFYSLLVTECEIFLSLLVKFLDGEKPQWLRAVAVESVHRLCVQPHLLRSFCQSYDMKQHSTKVFRDIVNALGSFIQSLFIVPSVGNASSTNTPTGGSASSAQASAQGGTGATGGSGGLTTQAAFEYRGTWIPLMTVSVQGSAKATYLEMLDKVEPPSIPEGYAMSVAFSALLDLVRGITSMIERELAKEEEEAAARVGSEPAASAQSEDSSQEPQPHQVWEEMVSACWCGLLAALSLLLDASTDETATESILKAELTMASLCGRLGLVTPRDAFITAICKASLPPHYALTVLSSNAANLSSKTYSIQGQNVQIISPSSESHQQVVAVGQPLTSQPQGTVVLTAKNIQCMRTLLNLAHCHGAVLGTSWQLVLATLQHLVWILGLKPGVGGALKPGRAVEGPSTVLTTAVMTDLPVISSILSRLFESSQYLDDVSLHHLINALCSLSMEAMEMAYGNNKEPSLFAVAKLLETGLVNMDRIEILWRPLTAHLLEVCQHPNARMREWGAEAVTSLIKAGLAYKHDPPLSQNQRLQLLLLNPLKELSNILHADIRQKQLECVLQILQSQGDSLGPGWPLVLGVIGAIRNDQGESLIRTAFQCLQLVVTDFLPTMPCTCLQIVVDVAGSFGLQNQELNISLTSIGLLWNISDYFFQRGEIITEELEKEEAVLLKQSRENGEPLNRPFHPAPPFDCLWLCLYAKLGELCVDPRPAVRKSAGQTMFSTIAAHGTLLQQATWHIVVWKVLFHLLDCVRKSSTTADKEKIESGGGNILIHHSRDTAEKQWAETWVLTLAGVARIFNTRRYLLQQLGDFFNAWEVLLDHIQSAALSKNNEVSLAALKSFQEILQLVTPVKDTDKPDALGAMDIPPVLMDPLTASGPGRPLPRSDSLAERLAQRYGAQPPPPPPGEEQDDSALWWSAWSTWYRTGIECTRPPSGGTDKLAFVPSQPFLTALIQIFPALYQHIAGGFSMEDLRKLGVILHGAVSVPISSDASPFILPSYTEAVLTSLQEAVLTALDVLQKAICVGPETLQVMYPAIFEQLLQFVEFSCKPPQYGKMETKHVANAKYNQASLQSTHAEWVALNYVPFAERSLEEVVDLYHKTACHKAVINEKVLQSIIKTLRMPLGLKYACPAESTWKLAVSSLLKVLSIGLPVARQQASSGKFDTMWPELANAFEDFLFTKSTPPDNLSIQEFQKNESIDVEVVQLISTEILPYANFIPKEFVGQIMAMLNKGSIHSQSSSFTEAEIDIRMREEFSKVCFETLLQFSFSNKASTPQEGYISRMALSVLLQRAQDVLRRYVEDERLSGRCPLPRQQVTEIIFVLKAVSTLMDSLKKTQPENVDGNTWAQVIALYPTLVECITCSSPEVSSALKEALGPFKDLMQPPVSKVQNGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZL4</accession>
    <name>A0AAR2IZL4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZL4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047470.1">
        <property type="protein sequence ID" value="ENSPNAP00000045188.1"/>
        <property type="gene ID" value="ENSPNAG00000034394.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271870"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="19134" checksum="F7A08F4D810E829E" modified="2024-11-27" version="1">MRNKILWSDETKIELFGVNARRHVWRKPGTAHHEANTIPTVKHGGGSIMLWGCFSAAGTGRLVRIEGKMNAAMYRDILDKNLLQSALDLRLGRRFIFQQDNDPKHTAKISKQWLQDHSVNVLEWPSQSPDLNPIEHLWRDLKMAVHRRLPSNLMERHRNTALYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZS9</accession>
    <name>A0AAR2IZS9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-type tyrosine-protein phosphatase kappa</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">PTPRM</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070433.1">
        <property type="protein sequence ID" value="ENSPNAP00000043096.1"/>
        <property type="gene ID" value="ENSPNAG00000024266.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155020"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14633">
        <property type="entry name" value="R-PTPc-M-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14635">
        <property type="entry name" value="R-PTPc-M-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000003">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000005">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000019">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000006">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase T isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000009">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000048">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045911">
        <property type="entry name" value="R-PTP-mu_cat_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF206">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043871124" description="Receptor-type tyrosine-protein phosphatase kappa" evidence="21">
        <location>
            <begin position="21"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="771"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="24">
        <location>
            <begin position="25"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="25">
        <location>
            <begin position="187"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="285"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="383"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="482"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="22">
        <location>
            <begin position="958"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="23">
        <location>
            <begin position="1132"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="22">
        <location>
            <begin position="1244"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="23">
        <location>
            <begin position="1420"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="899"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1510" mass="169942" checksum="A946DB83CED68925" modified="2024-11-27" version="1">MDKVFTCLVTAVSLLVSVRGQVFKGDCNFEKPFATCGYSQGRDDDLDWEQANTKEKPSANQWMPPGSAFMMVNTSGRFAGQKALFLSPQLKENDTHCVIFQYFVSGREGGSPGHLNIYIKENNSPMGLPVWNTSGPVSRSWIHVELAISTYWPNFYQIVFEVVTSGQRGLLAIKDVVVQGHQCMTTPHFLHIKGVEVNAGQTATFHCTVNGEWRDNFHLWLQGIGGREAPMKSTKPWNNQRFIGMFDVVNTTKQDSGRYRCIIHSNQGVGVSNYGELTIKQPPVPIAPPQLTAVGATYLWIQLNANSINGDGPIINREVEYRTVSGTMYDLQPVDKTSHKIGHLDPDTEYEISVLLTRPLEGGTGAPGPPLRARTKCAEPMHGPRQLKVVDIKSRQVTIRWEPFGYNVTRCHNYSLTVQYRARAGGKEESREEVFYDSKSSAPQHTIHNLTPYTNLSVRLVLRNREGVKESDEIHIQTDEDVPGEVPLDSIQASVYEEKIILKWREPAHTYGIITQYEISYKAVSSFDPVLDLSNQSGKVIKLGNETSHVFLGLYPGSTYSFTLRASTAKGYGPPAITQATTKISAPSMPAYDQETSLNQTDSTVTVLLKPAQSRGAPVSVYQVVVEEERPRRARGGEAEILRCYPVPVHYQNATSLNSKYYFTAEFPSGGIPSPQPFTVGDNKTYSGFWNAPLLPHKSYSIYYQAVSTANGETKIDCVRVATKAAILVTQLTTPYIRIAPAAGDNQLTGAATHKPNAVEPEKQTDHTVKIAGVIAGILLFVIIFLGVVLLMKKRRTYHSYTYYLKLAKKRKETLSSTRQEMTVMVNSMDKSYTEQGTNCDEALSFMDTHNLNSRSGSSPCSLTVKTNTLSTALPNSYYPDPFVPTAILVPINDETQTMASDTSSLAQSHTHSTHTHSLRKRDGVDVPYQTGQLHPAIRVADLLEHVTQMKCAEGYGFKEEYESFFEGQSAPWDSAKKDENRMKNRYGNIIAYDHSRVRLQSLEGEQSSDYINANYVDGYHRPNHYIATQGPMQETVIDFWRMVWQENTATIVMVTNLVEVGRVKCCKYWPDDTEIYRDMKVTLIETQLLSEYVIRTFAVEKRGAHEIREIQQFHFTGWPDHGVPYHATGLLGFIRRVKAKSPANAGPIVVHCSAGAGRTGCFIVIDIMLDMAEREGVVDIYNCVRELRSRRVNMVQTEEQYVFIHDAILEACLCGDTTIPANQLRSVYYDMNRLDPQTNSSPIKEEFRTLNMVTPTLRVEDCSIALLPRNHEKNRCMDVLPPDRCLPFLITIDGESSNYINAALMDSYKQPSAFIVTQHPLPNTVKDFWRLVLDYHCTSIVMLNDVDPAQLCPQYWPENGVHRHGPLQVEFVSADLEEDIISRIFRIYNAARPQDGYRMVQQFQFLGWPMYRDTPVSKRSFLKLIHQVDKWQEEYDGGEGRTVVHCLNGGGRSGTFCAISIVSEMLRHQHSVDVFHAVKTLRNNKPNMVDLLDQYKFCYEVALEYLNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZZ3</accession>
    <name>A0AAR2IZZ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ferroxidase</fullName>
            <ecNumber evidence="4">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZZ3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078994.1">
        <property type="protein sequence ID" value="ENSPNAP00000043604.1"/>
        <property type="gene ID" value="ENSPNAG00000027434.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158517"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000002">
        <property type="entry name" value="Hephaestin like 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024715">
        <property type="entry name" value="Factor_5/8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF233">
        <property type="entry name" value="FERROXIDASE HEPHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000354">
        <property type="entry name" value="Factors_V_VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043916299" description="ferroxidase" evidence="18">
        <location>
            <begin position="22"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1071"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="20">
        <location>
            <begin position="100"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="20">
        <location>
            <begin position="437"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="19">
        <location>
            <begin position="954"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="285"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="534"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="635"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000354-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1095" mass="123489" checksum="9F5096ACDA18FB2F" modified="2024-11-27" version="1">MEVRVNGWWLLCLLSFCCATGQNTGVNRTFYIGIREEIWDYCPGGLNRVNGKTAVEDEHASVFLQQGPHRIGHVYKKAMYRQYTDKSYTQEIPKPAWLAYLGPLIRAEVGDVIVIHLKNFASRRYSMHPHGVFYDKNSEGALYPDGTSGEQKQDDGIPPGETHTFNWMVKPEYAPTQGDANCLTWAYHSHVVASRDISAGLIGALLTCKKGSNYTQVQRTDVDQDILLMFSVVDENLSWYLQENIQTFCSDPAGVDINDPDFQLSNKKNTINGYMYGNLPGIELCQNRTVSWHLFGMGNEVDIHSAYFHGHTLLMRGHRVDAVSLFPATFLTAEMTPLTTGIWLLNCQVNAHIQDGMQALFHVSTCGMQEDTLASLSGTERKYFIAAELVQWNYAPSGKDTLTNVSLTESQSPSEVYFGKDGGRLGEEYVKVIFRAYTDHTFTNKTEQTSTDTHLGILGPVLRAEVGDVLQVTFLNKADRNYSIQPHGLQYMKTSEGAQYEDGTVKNGSHVQPRDTFTYRWQVREGPSDSDPPCISYLYFSSSDPVRDTNSGLIGPLLVCKRGALSSNNTEIDVDNEFFLLFSILDENLSWYLDKNIQIYGTNESNPQDDAFVESNKMHAVNGYMYGNLPGLDVCESSRVRWHLLGLGTEVDVHGVHFQGNTFLKEGTTRDTVTLFPHTSLSVLMQPDTVGLFEVSCRVSEHYMGGMKLQYRVRKCNESSTATPAVPTVYYFISAEEVEWDYSPTRTWELQNFNTTEDESPGSIFVGTGENRLGSKYKKVVYREYTDATFKIRKQRQTAEKHLEILGPIIRAEVGEVLQITFWNNASRPYSIHGLGLVMFVKNNMYPLSVGNVSSYLWSVPERSGPGTSDPNCITFAYYSAVDFIKDTVSGLIGPLVVCRKGMLSQNRQRLDVNQEFALLFFIFDENKSWYLEENIQTYYNSSVPLLRNDDFQESNKMHAINGKLYGNLHGLELMKGLKVNWYVLGMGSEVDMHTVHLHSQTFIYKTDLSHRADVLDLFPGVFQTLQIVPETEGTWLLHCHVSDHIEAGMETTYTIRSGTGETAASEYDGWNYFAVGPHLALIFILAFVSVLRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J292</accession>
    <name>A0AAR2J292_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SEC16A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Plays a role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">SEC16A and SEC16B are each present in multiple copies in a heteromeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">Belongs to the SEC16 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000071661.1">
        <property type="protein sequence ID" value="ENSPNAP00000046133.1"/>
        <property type="gene ID" value="ENSPNAG00000037695.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159324"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:vesicle-mediated transport"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="11" id="KW-0492">Microsome</keyword>
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    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
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            <begin position="1181"/>
            <end position="1278"/>
        </location>
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            <begin position="1349"/>
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            <dbReference type="RuleBase" id="RU364101"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF12931"/>
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            <dbReference type="Pfam" id="PF12932"/>
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    <sequence length="1817" mass="194875" checksum="BE2DCF2EEDB5EF8D" modified="2024-11-27" version="1">MLCNTVFPSIVLPQGQSSHTLSVLSSGGQHHLEEMQPPPRAAPPGACGPPPSSSGGPNAFRRTRPHKHGAAAAGAAAAAAAPVPPSQPVSDPFAFVRQVPPSMPVGSQAPPPNNSPLPMQAPQPAGLTLTAPQVPTSGGQGAAAGPPPTFFTPHPCPSGPSPAPNPPPAEPGYFNSQEPMPYIARLPNPTTPFSSSQFQSPPASLTPPIQSNPPFQPTPPAPSHLVPDHGSRPPSVQNYFQPTSDPPQPFHAPPQAQALHPAPSPHPGPPQAQALHPAPSPHPGPPQAQALHPAPSPHPGPPQAQALHPAPSPHPGPPLQAHLQNHSQSTPLNSGGPAGSQQQNFHFSAQNYFSQTGGAPEPWFSQVPQDPSQRACPIPYVPADSGTLSMFFQGNDVENEETLSGEGRVNGISHPPHAGAVAEASAQYLNDSGNIRAGAGPSDSVENLECIPNQEVLPSEPLPSLTYEAGPNLETPDSAPRPTRSASVSSSYSNVSHGSGHASHSGGHPPRRHQGVVGTFIQQESPRPPDSLASHSSPAGYFEQIDSAPAPEQSPTFPTPSPPKPVGVFQASANSSFEPVRSHSVGVRPAEVDRARMVVEKVGADVLPGNLEQPPDNMETFYFPGGQDRRPSSRAQGSRRPCESPATTLWAQSDPASLGANILLAPAAPPLAAALVPVRQSSAEVIQPPEDGPLDLQPPRTHPQPHSENLENPPDSASQASLGYASLLVATPSVEALNHPVLIAPPSSNYSVISSQSPPQTANQTSPRKATSPVQPPSQTPLASQLPPASSSNQLPPLFSQAPVSSYPRSSQSPLNLAREVKDAPPPGQSAHPPLSRAQSLRGDGQAVQSPNSQVTSSASTTNQNQPSNFELLDFSMHQPQSTNQPVALPISQSVPPAATVNNTPGFYLQVTKDAQQAGRADLEPPHQPPTPNPPRPSSNQTNSEPTGPSQSAHQIPTQYQPPPHAPPTSQAAPPPVSGTAPPPTYPPQPGNTTASHEPQRPLSSLGSQPGYGAPPSVPPGPGYAMTTSASRHFDMLVSMVSTIFRPEGYDDPWRYYPGYDSSFDDDCRRRDPYVDEFDRRSVHSERSAHSVHSAHSRRSSFSSRSQQSQVYRSQPDLVAASYDATATTLPVDYSYGQFPDQTDAQGYSQYNAAESTWTAPEQPPPRPLTPEKFSVPHCCARFGPAGQLILVQPNLPSAGQPALVELHSTETMMQDSPEQTELRAFPGPLVKEETHKVDVIKFAQNKAQECARNDDLLDKDSAVLVWEFIVLLCRQNGTVVGTDLADLLLKEHRSVWLPGKSPNEANLIDFTNEALERPEEEGSGPLSLLSDTFMGTPENAGKETERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDNRTHARVMTRFANSLPINDPLQTVYQLMSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTHTLDLDTRTISTMGDTLASKGLVDAAHFCYLMAQVGLGVFTKKSTKMVLIGSNHSLPFVRFCSTEAIQRTEAYEYAQSLGSQPLSLPNFQVFKFIYACRLAEAGLCAQAFHYCEVISRALLTLPDYHSPVFISQLIQMSERLRFFDPQLKEKPEQELFQEPDWLLLLRKLSSQIQVCGEAVKVKLDHASNLKSLILCSRRYYKPRLPDCGKNLPFIVWDEKKQRWVNLDEPEEEVSMWIGKYPQSSAEYVSCAFVYVSGTKGRYVDVLNQGRGDSKPAAAVPPPADLFAPLAPMAMPANLFVPTAGRSLRVNKSFNRKDFGLFVSSAPDDQQPMEGSVPDSSGANTEHTQPSATVPQVRTHRFFPSYDSENSTVMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDB4</accession>
    <name>A0AAR2JDB4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">G protein-coupled receptor 65</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JDB4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061123.1">
        <property type="protein sequence ID" value="ENSPNAP00000049930.1"/>
        <property type="gene ID" value="ENSPNAG00000037922.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286937"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF15">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="221"/>
            <end position="241"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="39"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="333" mass="37776" checksum="A9FBA7A371362AAE" modified="2024-11-27" version="1">MALNESMNKTNSQCTFNDTVDAYMYPTAYSLFFILGLPGNCLSLYVHIKQENELGVYLFNLALSDFFFIAGLPIWMDFELKDHWHHGKTLCTVCIFFLFTNFYSSALLLSCISVDRYFAVVYPLSFPFLRKPSTAATVSVAVWTFTLMFNAISIDPNQIYDDQFKICLDVFPISSKQIKVYVVSFFVGFLLPAVVVMFCYWRICREVRTNQATGLSERRRVFRLLGSVLLSLCLCFGPVHITMVLRAGLENVDTCPPAWLYFLYKAGVALAMLNCLADPLLYCFITRRGLYTMLTGSYKLPQASSLAGLMCRAHGLSSQCWLCEQHTLSSNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JKV3</accession>
    <name>A0AAR2JKV3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JKV3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081251.1">
        <property type="protein sequence ID" value="ENSPNAP00000050471.1"/>
        <property type="gene ID" value="ENSPNAG00000003355.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157665"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08403">
        <property type="entry name" value="C2B_Synaptotagmin-3-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000005">
        <property type="entry name" value="Synaptotagmin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000011">
        <property type="entry name" value="Synaptotagmin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF45">
        <property type="entry name" value="SYNAPTOTAGMIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="215"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="344"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="501" mass="56623" checksum="382C5C54EAFCA99B" modified="2024-11-27" version="1">FECSLFVSSSVTYICPVSALPPFLSISSSLDISFSVLLLVFVLCGLALLGVISLATWKLCWVPWRSEALSSSATALNPSRLDRERIRKVDQDYYSTQGHIMADDKLKGPGNFLEAAVKISHTSPDIPADVQLSMKDHLLRRTRIARQTTEPASSNRSHLPRQMHHVTSVDRGSDFLDVEDHSTCTAASLGRIQPELYKQSLQETEESSKNGAAKTCGKINFSLKYDYEGELLLVTILKAMDLPAKDLCGSSDPYVKIYLLPDRKCKFQTRVHRKTLNPIFDETFQFPVPYEQLTGRKLHLSVFDFDRFSRHDMIGEVILENLFEMSDLSRETSIWRDIQYESVDLGEIMFSLCYLPTAGRLTLTVIRCRNLKAMDITGYSDPYVKVSLICDGKRLKKKKTTIKKNTLNPTYNEAIIFDIPPENMEQVSLHISVMDYDLVGHNEVIGVNRVGSHAEGLGRDHWNEMLAYPRKPIAHWHPLLEQGRSLAFYSLASCLNVPVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JS05</accession>
    <name>A0AAR2JS05_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vesicular inhibitory amino acid transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Solute carrier family 32 member 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Vesicular GABA transporter</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SLC32A1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals. The transport is equally dependent on the chemical and electrical components of the proton gradient. May also transport beta-alanine. Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4-aminobutanoate(out) + n H(+)(in) = 4-aminobutanoate(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>beta-alanine(out) + n H(+)(in) = beta-alanine(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70987"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>glycine(out) + n H(+)(in) = glycine(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70983"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JS05"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057893.1">
        <property type="protein sequence ID" value="ENSPNAP00000054813.1"/>
        <property type="gene ID" value="ENSPNAG00000033621.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043380"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060077">
        <property type="term" value="C:inhibitory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140800">
        <property type="term" value="F:gamma-aminobutyric acid:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051939">
        <property type="term" value="P:gamma-aminobutyric acid import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000062">
        <property type="entry name" value="Vesicular inhibitory amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF689">
        <property type="entry name" value="VESICULAR INHIBITORY AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="490"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="21">
        <location>
            <begin position="115"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="525" mass="57540" checksum="C5B0FAA118F6D7DF" modified="2024-11-27" version="1">MAHLIRHKISNKITNAVNTASNKSQAKVSGVFARMGFQAATDEEAVGFAECDDLDYDYRQGMRMATLQTDEAGGEVDGEGGLEGDSHYQRDGAGPRGSRGSTGTCEELGSQDKPRITAWEAGWNVTNAIQGMFVLGLPYAILHGGYLGLFLIIFAAVVCCYTGKILIACLYEENEDGELVRVRDSYVDIANACCAPRFPALGGHVVNVAQIIELVMTCILYVVVSGNLMYNSFPGLPVSQKAWSVVATAALLPCAFLKNLKAVSKFSLLCTLAHFVINVLVIAYCLSRAHDWAWEKVKFYIDVKKFPISIGIIVFSYTSQIFLPSLEGNMQNPKEFHCMMDWTHIAACVLKGLFALVAYLTWADTTKEVITDNLPAGIRAWVNIFLVAKALLSYPLPFFAAVEVLEKSLFQDGGQALFPNCYGPGGQLKSWGLSLRVGLVVFTLLMAIFVPHFALLMGLTGSLTGAGLCFLLPSLFHLKLLWRKLLWHQVFFDVSIFVIGGICSISGFIHSIEGLIEAFRYNIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JVI3</accession>
    <name>A0AAR2JVI3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JVI3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086966.1">
        <property type="protein sequence ID" value="ENSPNAP00000056063.1"/>
        <property type="gene ID" value="ENSPNAG00000030184.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01080000257344"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001495">
        <property type="entry name" value="Si:dkey-234i14.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="107" mass="11934" checksum="507A56912691F44C" modified="2024-11-27" version="1">SYCDLKTVSPGDSVTISCRTSTAVYNNNRLSWYQQKPGEAPKLLIYYATSRQSGVPARFSGSGSGSDFTLTISNVQTEDAGDYYCQSYHYISIQIFTEVSVKLKHRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K472</accession>
    <name>A0AAR2K472_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TENT5A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:11333"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TENT family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2K472"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051422.1">
        <property type="protein sequence ID" value="ENSPNAP00000059055.1"/>
        <property type="gene ID" value="ENSPNAG00000033115.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108434215"/>
    <dbReference type="CTD" id="100330676"/>
    <dbReference type="GeneTree" id="ENSGT00940000157191"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012937">
        <property type="entry name" value="TET5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12974">
        <property type="entry name" value="PRION-LIKE- Q/N-RICH -DOMAIN-BEARING PROTEIN PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12974:SF25">
        <property type="entry name" value="TERMINAL NUCLEOTIDYLTRANSFERASE 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07984">
        <property type="entry name" value="NTP_transf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01153">
        <property type="entry name" value="DUF1693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="399" mass="45718" checksum="68F209CE688BF095" modified="2024-11-27" version="1">MAEEDSAAPGALQGSSFSVLSWEQVQRLDSLLTESIPIHGRGNFPTLQMKPRQIVKAVRRRMEERELRVRDVRLNGSAASHVLHEHSGLGYKDLDLIFCAELKGEAEFQMVKDIVLDCLLDFLPEGVNKEKITPLTLKEAYVQKMVKVCNDSDRWSLISLSNNRGKNVELKFVDSLRRQFEFSVDSFQIKLDSLLLFYECSENPMAKTFHPTIIGESVYGDFNVALEHLRNKVICTRNPEEIRGGGLLKYCHLLVRGFRAASETEMKSLQRYMCSRFFIDFSDINEQQRKLESYLQNHFVGLEDRKYDYLMTLHGVVNESTVCLMGHERRQTLGLIAMLAVRVLAEQNVIPNVANVTCYYQPAPYVADGNFSNYYIAQVQPVFACQQQQQTYSTWLPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K4P2</accession>
    <name>A0AAR2K4P2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent clpX-like chaperone, mitochondrial</fullName>
            <ecNumber evidence="2">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">ATP-dependent chaperone that functions as an unfoldase. As part of the ClpXP protease complex, it recognizes specific protein substrates, unfolds them using energy derived from ATP hydrolysis, and then translocates them to the proteolytic subunit (CLPP) of the ClpXP complex for degradation. Thanks to its chaperone activity, it also functions in the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis. This chaperone is also involved in the control of mtDNA nucleoid distribution, by regulating mitochondrial transcription factor A (TFAM) activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homohexamer that forms a ring structure; this hexamerization requires ATP binding. Component of the ClpXP complex formed by the assembly of two CLPP heptameric rings with two CLPX hexameric rings, giving rise to a symmetrical structure with two central CLPP rings flanked by a CLPX ring at either end of the complex. Interacts with TFAM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2K4P2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042201.1">
        <property type="protein sequence ID" value="ENSPNAP00000057429.1"/>
        <property type="gene ID" value="ENSPNAG00000007809.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017625"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19497">
        <property type="entry name" value="RecA-like_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000002">
        <property type="entry name" value="ATP-dependent Clp protease ATP-binding subunit ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial"/>
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    </dbReference>
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        <property type="entry name" value="ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ATP-dep_Clp_protease_ClpX"/>
    </dbReference>
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        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
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        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059188">
        <property type="entry name" value="Znf_CLPX-like"/>
    </dbReference>
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        <property type="entry name" value="Znf_ribbon_CLPX-like"/>
    </dbReference>
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        <property type="entry name" value="PRK05342.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26040">
        <property type="entry name" value="Zn_ribbon_CLPX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ClpX-type ZB" evidence="20">
        <location>
            <begin position="96"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="70"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="614"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="615"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00077425"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="637" mass="69613" checksum="F191E80C8A9E794C" modified="2024-11-27" version="1">MLLENSKLKTGVCPGLIHKCMLKFSAFAGFPCSRIQFFALSRPVSREAHLPAAVQIRSFSETAVCFASKENTPNSGSSDGGKKSMTDMAGKRSMGSGGSGKGGSQLRCPKCGDPCTHVETFVSSTRFVKCEKCHHFFVVLSETDSKKGLNKEAESAAEAVKLAFAQKPPPPPKKIFAYLDKYVVGQSYAKKVLAVAVYNHYKRIYNNLPAGGRQQVEAEKQSSLSPRELEIRRREDEYRFTKLLQIAGISPHGNALGASVQQQAAQQALQEKRGGEVLDSTHTDIKLEKSNIVLLGPTGSGKTLLAQTLARCLDVPFAICDCTTLTQAGYVGEDIESVIAKLLQDANYSVEKAQQGIVFLDEVDKIGSVPGIHQLRDVGGEGVQQGLLKLLEGTIVNVPEKNSRKLRGETVQVDTTNILFVASGAFNGLDRIISRRKNEKYLGFGTPSNLGKGRRAAAAADLANISGSETDAVAEIEEKDRLLRHVEARDLIEFGMIPEFVGRLPVVVPLHSLDEETLVRILTEPRNAVVPQYQALFSMDKCELNVTPDALRAIARLALERKTGARGLRSIMEKLLLEPMFEVPHSDIMAVELNKDVVLGKSKPCYIRAPAKETTEEEYDSGIEEENWPRQADVANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K8Z9</accession>
    <name>A0AAR2K8Z9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1731 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SDR39U1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017543344.1">
        <property type="nucleotide sequence ID" value="XM_017687855.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K8Z9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060291.1">
        <property type="protein sequence ID" value="ENSPNAP00000058959.1"/>
        <property type="gene ID" value="ENSPNAG00000033583.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108414933"/>
    <dbReference type="CTD" id="56948"/>
    <dbReference type="GeneTree" id="ENSGT00390000000337"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05242">
        <property type="entry name" value="SDR_a8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013549">
        <property type="entry name" value="DUF1731"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010099">
        <property type="entry name" value="SDR39U1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01777">
        <property type="entry name" value="yfcH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092:SF0">
        <property type="entry name" value="EPIMERASE FAMILY PROTEIN SDR39U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092">
        <property type="entry name" value="SUGAR NUCLEOTIDE EPIMERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08338">
        <property type="entry name" value="DUF1731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1731" evidence="2">
        <location>
            <begin position="252"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="32991" checksum="E88FF2098B2C7A37" modified="2024-11-27" version="1">MRVLIGGGSGFVGRELTRLLKSKGHEVTIISRQPGPGRITWADVESGGLPPCEGAVNLAGENIMNPLRWWNDSYKKDLFSSRVDTTRALAQAIAASSSPPRSWVLITGVACYKPSLQSQYTEDSEWTPFDLLSQLVKEWEEAGRLPHSLAQSTRQVVIRPGAVLGRDGGAMKQMLTPFWLGLGGPLGSGRQPFPWIHVSDLAGIILHALETSSSPPSTQLEVFNGVAPALNTNYEFTKELGRILRRPTLFPVPGFLMDTLLGSERAVILMQGQKVVPKRTLESGFQFRYPDLTSALREIVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KED1</accession>
    <name>A0AAR2KED1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CLASP1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Centromere</location>
            <location evidence="4">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CLASP family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000068921.1">
        <property type="protein sequence ID" value="ENSPNAP00000062640.1"/>
        <property type="gene ID" value="ENSPNAG00000014488.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154817"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000005">
        <property type="entry name" value="CLIP-associating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000006">
        <property type="entry name" value="CLIP-associating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000031">
        <property type="entry name" value="CLIP-associating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF28">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="19">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="17">
        <location>
            <begin position="168"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="19">
        <location>
            <begin position="308"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="19">
        <location>
            <begin position="1246"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="237"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="535"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="612"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="248"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="567"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="668"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="710"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1489" mass="165010" checksum="D8E35D1E04FD4583" modified="2024-11-27" version="1">METNVEYIVGQVLQKDLGRRLQVGQEIIDYVLDRQKSHDLEHDQTMLDRMVDGLATSWVNSSNFKVALLGMDIVSALVTRLQTRFKTQIGTVLPSLTDRLGDAKDQVRDQAQTLLLKIMDQAANPQYVWDRMLGGFKHKNNRTREAVCFCLISTLNIYGAQGLTLSKIVPHICNLLGDPTSQVRDGAMNCLVEIYRHVGERVRVDLGKKGLPQSRLNVIFSKFDEVQRSGNMILSADKNIDDDDSVDGGRSSASSKGASSARRTGSMRRPSSASSTKSTGKEGPSAGAVDEEDFIRAFEDVPTIQIYSSRELEDSMNKIREVLSDDKHDWEHRVAALKKVRSLLLAGATDHEGFLQQLRQLEGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAESIMPMLLNLVPNSAKVMATSGVAAIRLIIRHTHYHRLIPIMTISCTSKSVAVRRRSYEFLDLLLQEWQTNSLERHVSVLRETIKKGVHDADSEARSVARKCYWGFHGHFSKEAEQLFQALELTYQKALQSHLKSSDSIMSLPQSDRSSSSSQESLNRPLSAKVPVGSSVTRSKITSKVSSTPGSLQRSRSDIDVNAAASAKSRMPTVPAAAPFSSATALPPGSYASLGRVRTRRQSSGSSVGGGPSVTDSRGRSRAKVVSQSQRKFTPSRSAGSRSSSPGKLIGQSYGKVHHARGSGTTSEKRSKIPRSQGCSRETSPSRSGQVRGSRIPRPSMSQGCSRDTSRESSRDTSPARGFTPLDRFGLIHQARISASVNAMRILNTGMEVEAAVADALRRPMRRRYESPGMYSDDDANSDASSACSEWSCSSRNGGVPHYLRQTEDVAEVLNHCASSNWSERKEGLNCVFLLSYCDFSLAVFSMFLETLVDFITLHRDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDITRDSFPYDQQFNILMRFIVDQTQTPNLKVKVAILKYIESLARQMDPTDFVNSSETRLAVSRIITWTMEPKSSDVRKAAQMVLISLFELNTPEFTMLLGALPKAFQDGATKLLHNHLKNSSNASPLFLCFPPFLLLSSLSVFPPPCFIPRSWHSRLWGWSSNGPSKPPCPFTPQHPPGPATPPLRSLRRALSPSMLEYDTENMNSDEIYSSLRGVTEAIQSFSFRSQEDSIEPIKRDAKKDDACGVASSTADPRLTADVMEGGRMALDNKTSLLNTPSPRQFLGPRTREYNPYNYTDSISAYDKGALREAMFDDDVEQFRDGETFLVGDLLKELSNHSERVEERRAALLELLKVTRDDSVAVWEEHFKTMLLLLLETLGDKDHTIRALALRVLKEILRSQPARFKNYAELTIMKTLEAHKDSHKEVVRAAEEAASTLAASIHPEQCIKVLCPIVQTADYPIDLAAIKMQTKVIERIPQDSLHQLLPDIIPGLLQGYDNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDISSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKL8</accession>
    <name>A0AAR2KKL8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKL8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074807.1">
        <property type="protein sequence ID" value="ENSPNAP00000064883.1"/>
        <property type="gene ID" value="ENSPNAG00000034147.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278942"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38634">
        <property type="entry name" value="PROTEIN CBG16428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38634:SF1">
        <property type="entry name" value="PROTEIN CBG24827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="984" mass="106108" checksum="59C1D5619A9F7625" modified="2024-11-27" version="1">MQLNLKFSVKGLIKSIPNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNINPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKPTSTLNLNPNLKAKPTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSSLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKPTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKPTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNINPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKAKLTSTLNLNPNLKGVDNQLNITGKLINDIGVGRASIGDHPNIACFFIYFSFQKKPRKPGGFSAPRRAQACDKEVGGDFFLSYIKVEEQAHELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNA5</accession>
    <name>A0AAR2KNA5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Semaphorin-2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNA5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083845.1">
        <property type="protein sequence ID" value="ENSPNAP00000063586.1"/>
        <property type="gene ID" value="ENSPNAG00000002901.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156712"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000369">
        <property type="entry name" value="semaphorin-2A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000021">
        <property type="entry name" value="semaphorin-5B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000003">
        <property type="entry name" value="semaphorin-5B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000007">
        <property type="entry name" value="Thrombospondin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057563">
        <property type="entry name" value="Sema5A/B-like_TSP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF39">
        <property type="entry name" value="SEMAPHORIN-5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23260">
        <property type="entry name" value="TSP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="939"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="18">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="976"/>
            <end position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1058" mass="117467" checksum="CAAC0B26885C4B29" modified="2024-11-27" version="1">FSLSVTFSLSLCSPSLSLFLSLSLSLSLTWLSSFSISGVSDYSQLSVDLSRNQLIVGASAEWGVDEDTRRSCQSKGKTEATFLCQDLRQNLFLSQDECQNYIRVLLLSGPRLFTCGTNAFSPICTTRPILDLAQVVDSVNGVARCPYDPKHNSTAMLTDSGELYAATVIDFSGRDPVIYRSLGSMPPLRTAQYNSKWLNEPHFVSAYEVGRFTYFFLRETAVEQDCGKAVFSRVARVCQNDVGGRFLLEDTWTTFVKARLNCSRSGDVPFYYHELQSTFYLPEQDIIYGVFTTNVNSIAASAVCAFNLSGITQTFNGPFRYQENPRTSWLSTPNPIPNFQCGTVGDGGPGGNLTERSLQDAQRLFLMSDVVQPISVDPLISQDNIRFSKLAVDIVQGQETLYHVIYIGTEYGTIIKALSTSNNSLRGCYLEEMKLLPEEKWEPILNLQILQSDRSLFVGLNSRVLKIPLERCSNYQTQRECLEARDPYCGWDRKQRRCSTIEESTNMSQWIQNITHCPERNLTQDGRFGPWTPWQSCTHNDGGENTSACQCRSRSCDSPAPQCGGSECVGPTIEVANCSRNGGWTPWSSWSSCSTSCGVGFEVRQRSCNNPAPRHGGRVCVGQAREERLCNENTACPLPVSWVPWSSWSKCSAPCGGGVQSRVRSCENGNTCPGCSLEYRACNLESCAEVRRNTPWSPWYPVNVTVGGARQEQRVRYTCRALLPNPHDLQLGKRKLETRTCPGSEGPPGCETDGLVEDLVRVSRPPTRIQGATWSPWETWSSCSKECSRGFRTRKRSCLAPDGKSSPFSCSGTPVEYQDCNTQPCPVKGTWSCWSSWSECSTSCGGGHYQRSRSCSNPAPKHSGDICIGLHTEEALCNTQECEGGWGAWSEWSECEEGFQRRSRTCEISPAPCEGNSTNWRACGPSETVLSVSLSGFSLFHLLLTGVSCFLAALFGSVLVYVFCQRVQKPSQDSAVIHPSTPNHLHHKDPSGTPKNEKYTPMEFKTLNKNNLLPDESCNFFPSPLQQTNVYATTYYPTPLGKYDFPSLDSPCRSYMHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KRE9</accession>
    <name>A0AAR2KRE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cathepsin D</fullName>
            <ecNumber evidence="5">3.4.23.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Napsin-A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">May be involved in processing of pneumocyte surfactant precursors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.</text>
            <dbReference type="EC" id="3.4.23.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.5" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KRE9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070085.1">
        <property type="protein sequence ID" value="ENSPNAP00000066893.1"/>
        <property type="gene ID" value="ENSPNAG00000017899.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155733"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05490">
        <property type="entry name" value="Cathepsin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1960:FF:000001">
        <property type="entry name" value="Cathepsin D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000048">
        <property type="entry name" value="Napsin A aspartic peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000066">
        <property type="entry name" value="Napsin A aspartic peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033144">
        <property type="entry name" value="Cathepsin_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF42">
        <property type="entry name" value="CATHEPSIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07966">
        <property type="entry name" value="A1_Propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="13 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 20" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase A1" evidence="21">
        <location>
            <begin position="90"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="121"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="286"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="329"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="410" mass="44326" checksum="24EA80BA716E8279" modified="2024-11-27" version="1">MEQITGNIPCLPQVVLGLRDSVVGATDIKATSVLTIPLKKFRSIRRSMSDSGRALEELLAGSRSTKYNLGFPSSNGPTPETLKNYLDAQYYGEIGLGTPVQTFTVVFDTGSSNLWVPSVHCSLTDIACLLHHKYNGAKSSTYVKNGTAFAIQYGSGSLSGYLSQDTCTIGDIAVQNQIFGEAIKQPGVAFIAAKFDGILGMAYPRISVDGVTPVFDMMMSQKKVEKNVFSFYLNRNPDTQPGGELLLGGTDPKYYTGDFHYINISRQAYWQVHMDGMNIGSQLTLCKGGCEAIVDTGTSLITGPAAEIKALQKAIGAIPLIQGEYMVDCKKVPSLPTVSFTLGGKSYTLTGEQYILKESQAGKEICLSGFMALDIPPPAGPLWILGDVFIGQYYTEFDRENNRVGFAKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KT64</accession>
    <name>A0AAR2KT64_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KT64"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079349.1">
        <property type="protein sequence ID" value="ENSPNAP00000065689.1"/>
        <property type="gene ID" value="ENSPNAG00000010418.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167011"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000009">
        <property type="entry name" value="SEC14-like 2 (S. cerevisiae)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051064">
        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF83">
        <property type="entry name" value="SEC14-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="77"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="2">
        <location>
            <begin position="272"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="46995" checksum="BFD09AA70AFAEFFB" modified="2024-11-27" version="1">MSGRVGDLSPKQAEALDEFRERIGDVHDKLPIQSDHYLLRWLRARNFSINKAEAMLRKHVEFRKHMKVDSILEDWMPPEVIERYVSGGMCGYDREGSPVWYDVIGPLDPKGLLMSASKQDFLRTKIRDSEFLRRECLRQSEKLEKPVEAITLIYDCEGLGMKHMYKPAVEAYGEILTMFEDNYPEGLKRVLVIKAPKIFPIAYNLIKHFLCEETRRKIIVVGSNWKDVLQKYVAPDQLPVAYGGLLVDPDGDPYCKSKIKYGGVVPKSYYIRESVKVQYDNFVTISRGSDLQLEYEILVRNCLLRWQFAGDGADIGFGIYMKTKAGVKQKVGDMQEVFPTTRYNAHLVPEDGSYTCEEPGIYVIRFDNTYSMLQSKKISFSVDVVLPETHTNSEGQKQQMSTDVYFAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KTU0</accession>
    <name>A0AAR2KTU0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KTU0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070944.1">
        <property type="protein sequence ID" value="ENSPNAP00000065531.1"/>
        <property type="gene ID" value="ENSPNAG00000033208.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267406"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF102">
        <property type="entry name" value="IMMUNOGLOBULIN V-SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043602480" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="130" mass="14798" checksum="7ABB7BAAB733BBAE" modified="2024-11-27" version="1">MHYFCVISLMCSLTGCFTCIFAGDSGVTQTSRILWAQKGKYAEITCNHDKGGFYFHMYWFRQQQEKPMVLIVYTSTTSELDYGNFSQEKFSVTKERGESGSFTVKNVESDDSALYFCAVSEHSDTVLLHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KYP2</accession>
    <name>A0AAR2KYP2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RBMS3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KYP2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045019.1">
        <property type="protein sequence ID" value="ENSPNAP00000069468.1"/>
        <property type="gene ID" value="ENSPNAG00000026763.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157131"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12244">
        <property type="entry name" value="RRM2_MSSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000012">
        <property type="entry name" value="RNA-binding motif, single-stranded-interacting protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000014">
        <property type="entry name" value="RNA-binding motif, single-stranded-interacting protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="46"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="125"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="426" mass="46768" checksum="841D34D7861AC1C8" modified="2024-11-27" version="1">MYQYTYYYPHYLQTKQPYAPPQHPMAPPSPGSTSCNQSGVEQLSKTNLYIRGLSPTTTDQDLIKLCQPYGKIVSTKAILDKNTNQCKGYGFVDFDSPAAAQKAVSSLKATGVQAQMAKQQEQDPTNLYISNLPVTMDEQDLESMLKPFGHVISTRILRDASGVSRGVGFARMESTEKCEVVIQHFNGKYLKTPPAIPAPPEPLLCKFADGGQKKKPSQSKYTQNRAWSREGENGVTLTYDPAALQNGFYSSPYSFASNRMITQTSITPFITASPVSSYQVQSTSWMPHQQYVMQPTGTVITPSLEHTTVSMQPAHLISPLTQQMNHLTLGSTASVSIHTAPLTYYMTAASASVAPMQGTYIPPFTPVPPSALSVEGVVTDASPQTVAPSQELNTLQQQITMETSDHIPSYSYQSKNWSAEETKFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L0U6</accession>
    <name>A0AAR2L0U6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neurensin 1-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L0U6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075662.1">
        <property type="protein sequence ID" value="ENSPNAP00000068359.1"/>
        <property type="gene ID" value="ENSPNAG00000035110.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063877"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024883">
        <property type="entry name" value="Neurensin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14796:SF3">
        <property type="entry name" value="NEURENSIN 1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14796">
        <property type="entry name" value="NEURENSIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14927">
        <property type="entry name" value="Neurensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="21487" checksum="2D9E274664281AF1" modified="2024-11-27" version="1">MASCSEVCGSERSARFKSMAGSGCHGFGVRSYLHHFYEECTASMWDQEDAQSQATSPQWWSSGLCKVSLAFGAVVLAVGLVVLTVGFAVPSRIEAFGEGELLFVDRQAVQYNQGLHMCVQAGTGMLSLGGLLMAAGLLVSAFSRPAGREGEHSPQTIGRERKGGSGGQIGTNGRSNTSLVTKAPSPAAGDGAVPVSLSKLKSEIAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L316</accession>
    <name>A0AAR2L316_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L316"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082373.1">
        <property type="protein sequence ID" value="ENSPNAP00000070988.1"/>
        <property type="gene ID" value="ENSPNAG00000009483.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154360"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF541">
        <property type="entry name" value="PROTEIN TOLL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043378096" description="LRRNT domain-containing protein" evidence="10">
        <location>
            <begin position="33"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28042" checksum="8A6ADADC92D90BCE" modified="2024-11-27" version="1">MSTDLRADNECVGAICWLCALLSASLWVQVCAGCPEGCKCAWESKTVLCVSAGLLEVPRDLPLETVSLHLDHNFINSIPEDAFQDLLHLQALHLSHNKIDSLASGALRHLSTELRLLDLSYNQLRYARQEEFGVTRAKTRLYHNPWHCDCSLQQLIESLNLEPETVHEIVCKSSARGTNEGSRWEEAGPVTGHVGQPLVKLLDSGVNFCSLQRRTIDVAMLIIMFVWFFMVIGYVVYYMPMGKLCLWGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LAN7</accession>
    <name>A0AAR2LAN7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LAN7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053437.1">
        <property type="protein sequence ID" value="ENSPNAP00000073658.1"/>
        <property type="gene ID" value="ENSPNAG00000032927.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177531"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF102">
        <property type="entry name" value="IMMUNOGLOBULIN V-SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="117" mass="12793" checksum="B7A86ED604D7C3D9" modified="2024-11-27" version="1">MGAKTADSVKFQQVPSMIVNQIAIVKLQCSHDDNSLTVMLWYRQETASTTLTLIGYGYTAGTPTIETGFTDQFKMTSQNTTSGELIISDVKPSDSAVYFCAASLHSAQLRMTRSLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LC07</accession>
    <name>A0AAR2LC07_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metalloendopeptidase</fullName>
            <ecNumber evidence="10">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LC07"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083610.1">
        <property type="protein sequence ID" value="ENSPNAP00000074055.1"/>
        <property type="gene ID" value="ENSPNAG00000033217.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161051"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04283">
        <property type="entry name" value="ZnMc_hatching_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000040">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034039">
        <property type="entry name" value="ZnMP_hatching_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF839">
        <property type="entry name" value="HATCHING ENZYME 1.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043101441" description="Metalloendopeptidase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="11">
        <location>
            <begin position="60"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="264" mass="29492" checksum="D81ACE809A681F45" modified="2024-11-27" version="1">SNMEPTASLSILALLLLGLSQAVPLMETQHVDITRNILTINNGSRELLVEGDIVLPRTRNALVCPSNNCFWKKSSNGLVLVDLHNLYSPLPDLLMLLSDYLSVIAGAMATFHSKTCIRFVSRTFEADYLSIQSRDGCYSAVGRQGGVQVVSLSRNGCVYHGIAQHELNHALGFYHEHTRSDRDRYVRINWANISPTMQYNFNKENTNNLNTPYDYSSVMHYGKTAFSINGQETITPIPDPSVPIGQRVDLSAIDIKRINILYNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LC18</accession>
    <name>A0AAR2LC18_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 18">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="3 18">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Binds 3 Ca(2+) ions per subunit. Two of the Ca(2+) ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="3 18"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LC18"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065621.1">
        <property type="protein sequence ID" value="ENSPNAP00000072207.1"/>
        <property type="gene ID" value="ENSPNAG00000019852.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158392"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13363">
        <property type="entry name" value="PH_PLC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08593">
        <property type="entry name" value="PI-PLCc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000088">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000058">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000022">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028391">
        <property type="entry name" value="PLC-delta1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
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        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF210">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
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    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 18" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="10 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="20">
        <location>
            <begin position="28"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="23">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="22">
        <location>
            <begin position="520"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="636"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
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        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="549"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="576"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="679"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
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        <location>
            <position position="681"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
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        <location>
            <position position="705"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
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        <location>
            <position position="734"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
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        <location>
            <position position="735"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
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        <location>
            <position position="736"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
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        <location>
            <position position="196"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LPB8</accession>
    <name>A0AAR2LPB8_PYGNA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSPNAG00000031122.1"/>
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        <property type="component" value="Chromosome 6"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="125" mass="13656" checksum="97C0B95422DEEF22" modified="2024-11-27" version="1">IRNRSRRFKELLPPVGIGADVFKNSCLQSASRHRSRCFKELLPPVGIGADVLKNSCLQSASDRHRSRCSKELLPPVGIGADILKNSCLQSASEPTRRFKELLPPLGIGADVLKNSCLQSASEPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LUX7</accession>
    <name>A0AAR2LUX7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="primary">HGH1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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            <taxon>Characoidei</taxon>
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        </lineage>
    </organism>
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            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
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        <property type="nucleotide sequence ID" value="XM_017718963.2"/>
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        <property type="nucleotide sequence ID" value="XM_017718964.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037535206.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSPNAT00000062647.1">
        <property type="protein sequence ID" value="ENSPNAP00000078457.1"/>
        <property type="gene ID" value="ENSPNAG00000033081.1"/>
    </dbReference>
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    <dbReference type="CTD" id="51236"/>
    <dbReference type="GeneTree" id="ENSGT00390000016546"/>
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        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04063">
        <property type="entry name" value="DUF383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04064">
        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="3">
        <location>
            <begin position="99"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="4">
        <location>
            <begin position="275"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="382" mass="43791" checksum="2D18F1F0D3C1455B" modified="2024-11-27" version="1">MLSDSEATELLSFLRRDMRADVKGQATEYILGLTGNREGCRYLRTKPDFLKALVTLTADPSIAIVKDCYHALVNLSADETLHKPLVKEAAILPTMFHNLLDPEYMFADRVSTILTNLSRHEATCREVFQALQEQEISLAKIVEIFCTEGFNKKASLHYLGPLLSNLTQLPEARQFILDKERCVIQRLLPYTQYEASVTRRGGVVGTLRNCCFDYAHHEWLLSDAVDILPFLLLPLAGPEELSEEENEGLPVDLQYLPEDKTREEDPDIRKMLIETLLLLTATKKGRQIMKEKNVYPIMREFHKWEKDLQVISACEKLVQVLIGDEPEPGMENLMEVNIPEGVEQKFKDLDAKEQEEIEKEKQELLKEEAEKHPNTVPEVLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LVN8</accession>
    <name>A0AAR2LVN8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CACNA2D3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel subunit alpha-2/delta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LVN8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071457.1">
        <property type="protein sequence ID" value="ENSPNAP00000080630.1"/>
        <property type="gene ID" value="ENSPNAG00000026389.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155766"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12912">
        <property type="entry name" value="PDC2_MCP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01463">
        <property type="entry name" value="vWA_VGCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000012">
        <property type="entry name" value="Calcium channel, voltage-dependent, alpha2/delta subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000007">
        <property type="entry name" value="Calcium voltage-gated channel auxiliary subunit alpha2delta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF25">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1089"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="19">
        <location>
            <begin position="275"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1110" mass="125654" checksum="0B1049A7908EFA16" modified="2024-11-27" version="1">MYQKGRTFQALAREGVFVKMRGVKVVPARLRCGLFLFMWIIQSTAVVDGAGTHQGIPPQVVKLWASAFGGEMKSIAAKYSGSQLLQKKYKEFERSVRVEEIDGLKLVKRLAENMEEMFRKKAEAMKRLVEAAEEAHLQHEEDPNLQYEYFNAVLINEQDEDGNSVELGGEFILQPNDHFNNLSVNLSLSVVQVPTNMYNKDSAIVNGVYWSEALNKVFVDNFERDPSLIWQYFGSAKGFFRQYPGIKWTPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIAKQTVSSILDTLGDDDFFNIIAYNEELRYVEPCLNGTLVQADVTNKDHFREHLDKLFAKGIGMLDRALTEAFELLSDFNQTGRGSVCSQAIMLVTDGAVDTYDAVFAKYNWPDRKVRIFPYLIGRESAFADNLKWMACANKGYFTQISTLADVQENVMEYLHVLSRPKVIDQEHDVVWTEAYIDSTLPQAQKLEDNQGPVLMTTVAMPVFSTKNETKNKGILLGVVGTDVPVSELLKTIPKYKLGIHGYAFAITNNGYILTHPDLRPLYEEGKKRRKPNYSSVDLSEVEWEDKDDELRNAMVNRKTGIFSMEVKKTVDKGKRVLVLHNDYYYTDIKGTPFSLGVALSRGHGKYFFRGSVTVEEGLHDLEHPDVALADEWTYCNTDEHPEHRYLSQIEAIKLYLSGEQPHLKCDKELIQEVLFDAVVTAPIEAYWTSLALNKSENSDKGVEIAYLGTRTGLSRINLFVVPDQLSNQDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFIYSLPFNTGSENKSVVLASTAIQLLDERKSPIAAAVGIQMKLEFFQRKFWTASRQCAALDGKCSISCDNDDINCYLIDNNGFILVAEDSSLTSVFFGEAEGAVMSKLLTMGSFKRVTLYDYQAICRIYAESSDSSSSLLDPYFAFFAAVKWLLTELVIFLVEFNLYSWWHSDFTAKAQRDGRTMLVPCDTEYPAFISERTIKETTGNIGCDDCNKSFVIQQIPSSNLFMVVVDNKCTCRDFGPITMDPIEIMYNESLKCERLKLQKHRRRPDTCHPFHPEENSMECGRALGLTASLTVTFFALLVALFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M155</accession>
    <name>A0AAR2M155_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SOCS7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M155"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057719.1">
        <property type="protein sequence ID" value="ENSPNAP00000080642.1"/>
        <property type="gene ID" value="ENSPNAG00000037708.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000284445"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="379"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="717"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="804"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="862"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="906" mass="96347" checksum="D27773CA743F7EC3" modified="2024-11-27" version="1">QPSPTPTSMHVPAMPSSLGRRSKCKQRGESPCSAANKFSNGCVLFLAPGDRLSSPRLEPLSPTPVEPLSSPEFGPLFPAPGDRLSSPQLDPLSSAEFGPLFPTPGDRLSSPRLDPLSSAEFGDRLSSPQRKPLSPTPVEPLSSPEFGQLFPTPGDRLSSPQLDPLSSPLFGPLFPTPGDRLSSPRLDPLSSPEFGPLFPTPGDRLSSPRLDPLSSPEFGPLFPTTGDRLSSPQRKPLSPTPVETLSSPEFGPLFPTPGDRLSSPRLDPLSSAEFDPLFPTPGDRLSSPRLQPLSSAEFGPLFPTTGDRLSSLRLGALSSPVFGPLFPTPGDQISSPRLDPLSSPEFGPLFPTPGDRLSSPRLEPLSSPVFGPLFPTPGDRLSSPRLDPLSSAEFGDRLSSPRLDPLSSAEFGPLFPTPGDRLSSPQRKPLSPTPVEPLSSPEFGPLFPTPGDRLSSPRFDPLSSPVFGPLFPTPGDRLSSPRLDPLSSPVFGPLFPTPGDRLSSPRLDPLSFPVFGPLFPTPGDRLSSPRLDPLSSPEFGPLFPTPADQISSPQRKPLSPTPVEPLSSPEFGPLFPTPGDRLSSPRLDPQSSPEFGPLFPTPGDQISSPQRKPLSPTPVEPLSSPEFGPLFPTPGDRLSSPRLDPLSSPEFGQLFPTPGDRLSSPQRKPLSPTPVEPLSSPEFGPLFPTPGDRLSSPRLDPQSSPEFGPLFPTPGDRLSSPRLGPLSSPVFGPLFPTPGDRLSSPQWKPLSPTPVEPLSSPEFGPLFPTPGDRLSSPQWKPLSPTPVEPLSSPEFGPLFPSPGDRLSSPRLDPLSSPEFGPLFPTPGDRLSSPQWKPLSPTPVEPLSSPEFDPLLPTPGVQPPTSQWEQLSPTPTSMQVTAMPSSLALHSNFKKGEALCSAANSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M3V2</accession>
    <name>A0AAR2M3V2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vesicle associated membrane protein 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M3V2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063912.1">
        <property type="protein sequence ID" value="ENSPNAP00000081824.1"/>
        <property type="gene ID" value="ENSPNAG00000035833.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164382"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043860107" evidence="4">
        <location>
            <begin position="19"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="91" mass="10624" checksum="EABE277A1FB3AB35" modified="2024-11-27" version="1">MVLYFILLLLSAHHFGLICSPVQVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFESCAAKLKNKYWWKNCKVRVLIRYFVHLSLWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNX9</accession>
    <name>A0A3B4BNX9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNX9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001457"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012123.2">
        <property type="protein sequence ID" value="ENSPNAP00000001457.2"/>
        <property type="gene ID" value="ENSPNAG00000000745.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160802"/>
    <dbReference type="OMA" id="PICGQSH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031102">
        <property type="term" value="P:neuron projection regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000445">
        <property type="entry name" value="Oligodendrocyte myelin glycoprotein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051071">
        <property type="entry name" value="LRR-bact_E3_ubiq_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47114">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47114:SF4">
        <property type="entry name" value="OLIGODENDROCYTE MYELIN GLYCOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043456105" description="LRRNT domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="5">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001457.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="390" mass="43407" checksum="F578965EFA7F8B17" modified="2024-11-27" version="2">MESTWIILLLLLLCAGYVPAICPPMCSCGRGHRVVDCSARGLSLLPDSLQHNIHFLNLSHNRLQDLDGLLGHFGHLRTLDISHNRLMHLPDGLPRALWDIHASANDIRQLVKNDTAYHWNLQALDLSNNQLERVVFINNTLPSLRALNLSHNKFWTVPTNMPHNLEIVDLSHNFLLQILPGSLDRLHQLSRFYLHANRFASVSEGVFNRLESLQLLTLGDNPWACEDEENMTHLLNWVHQTPAKVLGCPCYVRPTCGEAHLATSRTWHSASYTEPPLGTDTRDSGHEPPIQVVTSVYLSKSALLNLPYNRSTGNATGAGEQALTKGGGFLTSTPHTLSTQSSTTIRTRSTKKAIPGKARSISIQMHTCSPHTVALNLLILHNIYCLLQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BP13</accession>
    <name>A0A3B4BP13_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DNER</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017542423.1">
        <property type="nucleotide sequence ID" value="XM_017686934.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BP13"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000179"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014296.2">
        <property type="protein sequence ID" value="ENSPNAP00000000179.1"/>
        <property type="gene ID" value="ENSPNAG00000007330.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414131"/>
    <dbReference type="CTD" id="92737"/>
    <dbReference type="GeneTree" id="ENSGT00940000158872"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035282">
        <property type="term" value="P:segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="Delta and Notch-like epidermal growth factor-related receptor"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000566">
        <property type="entry name" value="delta and Notch-like epidermal growth factor-related receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000609">
        <property type="entry name" value="delta and Notch-like epidermal growth factor-related receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000377">
        <property type="entry name" value="Delta/notch like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000703">
        <property type="entry name" value="Delta/notch like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000597">
        <property type="entry name" value="Delta/notch-like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045769">
        <property type="entry name" value="DNER_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051830">
        <property type="entry name" value="NOTCH_homolog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24033:SF227">
        <property type="entry name" value="DELTA AND NOTCH-LIKE EPIDERMAL GROWTH FACTOR-RELATED RECEPTOR PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24033">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19330">
        <property type="entry name" value="DNER_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017342021" description="EGF-like domain-containing protein" evidence="10">
        <location>
            <begin position="35"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="637"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="43"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="84"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="306"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="345"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="388"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="426"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="466"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="503"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="541"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="579"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
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    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="470"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="735" mass="79882" checksum="F48925037D7DD941" modified="2018-12-05" version="1">MVMVMMIWMRLPTAFLPPLLFLTIYSMFLTPVTPAVTSAPTDPPDPCEGWPCLNGGVCSSLLDSQWTYTCTCRPAFTGHNCEFFADPCTSGPCLHGNCSRDTNGAGFVCECNDGYGGVRCDQAMTTFDLPESWDLAESHAPESVTPVTPATTTQPSQPITANPTTQAPPTLQPWQPKPGQRVVEVQWDEQQITDGSECGSSAGPDSAGTMTVSMEAAEETAVKLVVNISGPAVLWRVKDEGYSHCSVDDGTIVWFQQAADGLVIPDQSLPLGHNYFITVLRVGAPSAPEVTVRLQLTVKASSCMEPGSGFSDPQCSGRGRCITQPSASTFYCQCDDGFSGIFCEEFDACHTKPCENNGTCTDLRQGDEGRNYTCICPPGFEGERCQAVVDHCASEPCRNRATCTSTLDGPRCYCTEGYQGSACEVRVDPCLSTPCHNNGTCYPQVGGRGFSCTCPAGFTGPTCGQLVDFCALNPCAHGICRSAGTSYRCLCVPGYHGLYCEEEYNECLSAPCQNYATCRDLINAYECICTPQYTGRHCEVYRDPCVRLQCQNGGRCESRGSNTSCVCTPGYKGVNCEVDINECESNPCHHGGTCIDQSNDFTCHCPPGWVGGNCEIHLQWKMAHPVDSLTNMPRHSLYIIIGALCVAFVLMLIILIVGICRISRIEYQASSRHAYQEFYNCRSIDSDFSNAIASIRHARFGKKSRPAMYDATPISYEDYSPDDKPLVTLIKTKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPC8</accession>
    <name>A0A3B4BPC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EFCC1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017548325.1">
        <property type="nucleotide sequence ID" value="XM_017692836.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPC8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000309"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014064.2">
        <property type="protein sequence ID" value="ENSPNAP00000000309.1"/>
        <property type="gene ID" value="ENSPNAG00000000187.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424666"/>
    <dbReference type="CTD" id="79825"/>
    <dbReference type="GeneTree" id="ENSGT00940000163561"/>
    <dbReference type="OMA" id="RCDDKAF"/>
    <dbReference type="OrthoDB" id="10054715at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031601">
        <property type="entry name" value="CCD48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15799">
        <property type="entry name" value="CCD48"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="477"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="624" mass="71120" checksum="FA0C8D87D7B72842" modified="2018-12-05" version="1">MEISDACDPYRRPARRTQWIVSTLAYHYGLDRGVENEIIVLATGLDQYLQEIFHHLDCQGDGKIPAEDFRILCEVLGLEAEAEPEECSGILESLPVEFTFRQFHAKLCGYFSTKAGRCYEDGRLLVGGDSEHIETQIRLRSPLRRRGKLLTAAGSAASPGDSSCKAGSCTRECYEEIVALEEAEDRMAKLEEENASLRELVEDMRAALQSSDARALALQVGLWKSHAKHRPDSGCFIGHHKRAAQKFKCQGNLKSVQGFQREMEMAQVGQVEEALLLKQKRLEQELRGSREATLMLEECNRTLKKEHMEMRKKVEEARQALFSGLAKVKELEAKASQIPTLQRHVQQLEMELLYYRSEVSKVSVVFSSVEEMQYSRQNRRCCLDSQRDRDSPTGRAENAHDNVEEQLFRSVEGQAASDEEEERWTGEQQRQVDEVKRILTRLSCCGDRCDERALKRLLSHCGESRAEESYTAVLELLDRVTRLHKQLELRESQARRDHPDPEQIKDALLHELQQKAEETEMLHRELQMLETERVRLSLVEEKLMDVLQLLQQLRELNVSRRSLGKILLSTLESCSDPQHGKAHILEVLNALCHELAACEILSASATLEKAQSHQSLTNSLLISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPK9</accession>
    <name>A0A3B4BPK9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-box/LRR-repeat protein 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">F-box and leucine-rich repeat protein 20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">FBXL20</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with SKP1 and CUL1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017578258.1">
        <property type="nucleotide sequence ID" value="XM_017722769.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPK9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001518"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012021.2">
        <property type="protein sequence ID" value="ENSPNAP00000001518.1"/>
        <property type="gene ID" value="ENSPNAG00000008217.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442633"/>
    <dbReference type="CTD" id="84961"/>
    <dbReference type="GeneTree" id="ENSGT00940000153845"/>
    <dbReference type="OMA" id="LCNRIRY"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22115">
        <property type="entry name" value="F-box_FBXL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000339">
        <property type="entry name" value="F-box/LRR-repeat protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000578">
        <property type="entry name" value="F-box/LRR-repeat protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000042">
        <property type="entry name" value="F-box/LRR-repeat protein 20 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000013">
        <property type="entry name" value="F-box/LRR-repeat protein 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF47">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="10">
        <location>
            <begin position="22"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="436" mass="48349" checksum="154F40CA343F602B" modified="2018-12-05" version="1">MGKEVNGVSRSRFEMFSSSDEAIINKKLPKELLLRIFSFLDVVTLCRCAQVSRSWNVLALDGSNWQRIDLFDFQRDIEGRVVENISKRCGGFLRKLSLRGCLGVGDSALRTFAQNCRNIELLSLNGCTKITDSTCNSLSKFCPKLKHLDLASCTSITNLSLKALSEGCPLLEQLNISWCDQVTKDGVQALVRCCPGLKGLFLKGCTQLEDEALKHIGAHCPELVTLNLQTCSQITDEGLITICRGCHRLQSLCVSGCANITDAILNALGQNCPRLRILEVARCSQLTDVGFTTLARNCHELEKMDLEECVQITDATLIQLSIHCPRLQVLSLSHCELITDDGIRHLGSGPCAHDRLEVIELDNCPLITDASLEHLKTCHSLDRIELYDCQQITRAGIKRLRTHLPNIKVHAYFAPVTPPPSVGGSRQRFCRCCILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQK5</accession>
    <name>A0A3B4BQK5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RUN and FYVE domain containing 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RUFY2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017571366.1">
        <property type="nucleotide sequence ID" value="XM_017715877.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQK5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000870"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013117.2">
        <property type="protein sequence ID" value="ENSPNAP00000000870.1"/>
        <property type="gene ID" value="ENSPNAG00000007790.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438201"/>
    <dbReference type="CTD" id="55680"/>
    <dbReference type="GeneTree" id="ENSGT00940000155595"/>
    <dbReference type="OrthoDB" id="79871at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15759">
        <property type="entry name" value="FYVE_RUFY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17695">
        <property type="entry name" value="RUN_RUFY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000001">
        <property type="entry name" value="RUN and FYVE domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000046">
        <property type="entry name" value="RUN and FYVE domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047333">
        <property type="entry name" value="FYVE_RUFY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047335">
        <property type="entry name" value="RUFY1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047332">
        <property type="entry name" value="RUN_RUFY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45956:SF3">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45956">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RUN" evidence="8">
        <location>
            <begin position="125"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="630"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="302"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="696" mass="79105" checksum="3AF5B22EC8F78571" modified="2018-12-05" version="1">MATPAEPEMSLAEAEGSKEKTHVFGILRLQEEKGGGEKAGVKPGDGRWQAPIFALARKASETISGGIHGLPKAAEPKASPFPPHEWSGTAFRDPMAMERANLLNMAKLSIKGLIESALSFGRTLDSDYPPLQQFFVVMEHCLKHGLKVKKTFLGYNKSLWGPLEMVEKLCPEAGEIAASVRDLPGLKTPLGRARAWLRLALMQKRLADYLRVLITRKDILSDFYESSAVMLEEEGAVIVGLLVGLNVIDANLCVKGEDLDTQVGVIDFSMYLKNDIDDYRTEERNGQIAAILDQKNYVEELNRQLNSTVQGLQGRVDSLEKSNSKLIEELAIAKNNIIKLQEENHQLRTENSVILMKAQQRLEVTKGDVNCELDTYKQSRQGLDEMYNEARRQLREECQLRQDVENELVVQVSMKQEMELAMKLLEKDIHEKQDTLIGLRQQLDEVKAINVEMYQKMQSSDDGMKQKNDMIARLEEKTNQITATMRQLEQRLQDAERDRLSAEDGMRRFKQDFANKADSLQKQIGQREKQMLQLETDLKIEREWRQTLQNDLERERDTVAQLNAEAQQINALKKEFHRLQDENTQLKGICEEQEQALEELGCKLSESKLKIEDIKEANKALQGGQVWLKDKDATHCKLCEKEFSISRRKHHCRNCGEIFCNACSDNELPLPASPKPVRVCDTCHALLLQRCSSNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRS3</accession>
    <name>A0A3B4BRS3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EVX1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the even-skipped homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRS3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012655.2">
        <property type="protein sequence ID" value="ENSPNAP00000001149.2"/>
        <property type="gene ID" value="ENSPNAG00000007983.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159854"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072498">
        <property type="term" value="P:embryonic skeletal joint development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033334">
        <property type="term" value="P:fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021521">
        <property type="term" value="P:ventral spinal cord interneuron specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000256">
        <property type="entry name" value="Even-skipped homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052002">
        <property type="entry name" value="Even-skipped_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294:SF2">
        <property type="entry name" value="HOMEOBOX EVEN-SKIPPED HOMOLOG PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294">
        <property type="entry name" value="SEGMENTATION PROTEIN EVEN-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="30"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="32"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001149.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="258" mass="27977" checksum="D6E13851CA3060D1" modified="2024-11-27" version="2">IGAPQGLGKESGADSGKLSSSPNALSYGPDQMRRYRTAFTREQIARLEKEFYRENYVSRPRRCELAAALNLPETTIKVWFQNRRMKDKRQRLAMTWPHPADPAFYTYMLSHAAATAGGLPYPFPSHLPLPYYPPLGGAASTGGAPLIAGHAAARSLEGLRVLSSHHPYPRPELLCAFRHPSLYAGATHALGPSSSPCSCLACQTSGAPPPPPPPPPPHPRSANADFSRAEGFITFTPAVLSKPTSVALDQREEVQLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRY9</accession>
    <name>A0A3B4BRY9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Neurotrypsin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Serine protease 12</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in neuronal plasticity and the proteolytic action may subserve structural reorganizations associated with learning and memory operations.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571301.2">
        <property type="nucleotide sequence ID" value="XM_017715812.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRY9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012326.2">
        <property type="protein sequence ID" value="ENSPNAP00000001345.2"/>
        <property type="gene ID" value="ENSPNAG00000000696.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438184"/>
    <dbReference type="GeneTree" id="ENSGT00940000164412"/>
    <dbReference type="OMA" id="ALVRMWG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000053">
        <property type="entry name" value="Neurotrypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000005">
        <property type="entry name" value="Neurotrypsin isoform A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF22">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0420">Kringle</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043971827" description="Neurotrypsin" evidence="14">
        <location>
            <begin position="25"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="15">
        <location>
            <begin position="54"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="17">
        <location>
            <begin position="123"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="17">
        <location>
            <begin position="232"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="17">
        <location>
            <begin position="339"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="17">
        <location>
            <begin position="459"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="16">
        <location>
            <begin position="588"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="148"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="161"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="257"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="270"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="484"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="497"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001345.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="834" mass="91725" checksum="17B331DED32CB131" modified="2024-11-27" version="2">MATRTAGGISAVLSLCWFLLHVLGDDTQQDGHLNSLQSPGPLSCSEGFTELGYYNGSVSQTESGFPCLKWTEFPDYMLQYPNRGLGHHSYCRNPDREVNPWCFFRQRSGAIGWAYCDCHQGAARLMGGPSNKSGRLEVYLNGRWGAVCDTHLTDRDASVICRQLGLGEIGTALQHSYFGPGSGLFHYERLGCCGNENSLVECRNRKFVSGDCNHGNEAGVVCAAPEGNGTPLRLVGGLEEFEGRVEVYHKGKWGTICDDQWDDIDAEVVCRQLGLGGVPKAWMWSHFGQGSGPILLNRVQCTGNELSLEECPHMPWGQHNCDHMEDAGVSCNPYTDGVLRLVGADGPWEGRLEVYHSGEWGTVCDDNWTERHAQVVCRQLGFSRGRAEVAPDGAYGEGTGLILLDEVTCEGGESSLLECEHAEWGRHDCSHSEDVGVRCERAVDTNDIPAVPPVSGPLVRLVSGESRKEGRVEVFLNGQWGSVCDDGWNDINAGVVCRQLGFIGVSKARSMAYFGEGQGPIHLDNVRCVGTESSLGECPADGPGTHDCRHSEDAGVICDYVSQPADDSAMAKQSCGLRANTQRRRRRIIGGDKSLRGDWPWQVSLWLRSQSKRNHPLCGATLINSCWVVTAAHCFKRFGSDPSRYVLRLGDYHTEERDDFERTLSPERIVIHKKYQSQGWEYDIALLRLKGTEGKCVSFNPHTNAACLPAQSNRRAKRPAACVITGWGVTDSEYSRTLLQAWLPLLPSWKCKKRYGSRFTGRMLCAGSLSNHRRVDSCQGDSGGPLVCQGQGGRWTLTGIISWGHGCGDPSYPGVYTRVSRFLKWIEKVTLTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZD1</accession>
    <name>A0A3B4BZD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Coagulation factor VII</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">F7</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557288.1">
        <property type="nucleotide sequence ID" value="XM_017701799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZD1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005062"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005929.2">
        <property type="protein sequence ID" value="ENSPNAP00000005062.1"/>
        <property type="gene ID" value="ENSPNAG00000011383.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429788"/>
    <dbReference type="CTD" id="2155"/>
    <dbReference type="GeneTree" id="ENSGT00940000154474"/>
    <dbReference type="OMA" id="QGRNCET"/>
    <dbReference type="OrthoDB" id="10004439at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000513">
        <property type="entry name" value="Coagulation factor VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000013">
        <property type="entry name" value="Coagulation factor X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000434">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF26">
        <property type="entry name" value="COAGULATION FACTOR VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017415747" description="Coagulation factor VII" evidence="16">
        <location>
            <begin position="19"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="19">
        <location>
            <begin position="38"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="84"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="18">
        <location>
            <begin position="197"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001143-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="49096" checksum="8EC4DBD6FAFA6D34" modified="2018-12-05" version="1">MQLLSLFSLILSLCCCHSASVFVKRNEAHGVLTRSKRANSGWFEEMKMGNLERECLEEKCSYEEAREVFEHAEVTNEFWKVYNAEDHCQSNPCENNGVCSPLKESYACLCPPGFSGRNCERVIKDIPDSCLHNNGGCEHFCEEQKGQRNCSCADGYFLGTDGQSCLSQETFPCGKVPLLQSNLTSKKALEQDPRARIVGGTECPRGHCPWQVLLKYGEKGFCGGAIIKPTWIVTASHCLEKLLVKFLKIVAGEHDIDVNEGSEQTIEVEEIIMHPNYISATADSDIGLLRLRRPIVYSTYAVPVCLPHKVFAERELWAVHLHTVSGWGRRSEGGPTSRVLRKLEVPRIRTQQCVEQSNVSLTANMFCAGYFEGNQDSCKGDSGGPLVTHYRNTTFLLGVVSWGKGCARPGHYGIYTRVSNYLEWIHKYTSAPGVNSMKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZK6</accession>
    <name>A0A3B4BZK6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LRRC42</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LRRC42 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561783.1">
        <property type="nucleotide sequence ID" value="XM_017706294.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZK6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005137"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005809.2">
        <property type="protein sequence ID" value="ENSPNAP00000005137.2"/>
        <property type="gene ID" value="ENSPNAG00000011453.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432464"/>
    <dbReference type="CTD" id="115353"/>
    <dbReference type="GeneTree" id="ENSGT00390000002727"/>
    <dbReference type="OMA" id="FYGKTHR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039631">
        <property type="entry name" value="LRRC42"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31994">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31994:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="370"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005137.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="413" mass="47310" checksum="A0538DC494BA165B" modified="2024-11-27" version="2">MWSEHGSVYVREKGELHCVRTDGQLLSTYKAPRLFQKDFTVRLCIESSAPISRQRSDHFIFTYNREGSLCYTVKPLLDISLLFIADNIEHVDSLVGFPEQMAHKLFVVAEEKQKFTDPPTSARALQVFSEAYGGLVLKSLCLRDRKLLVSERMDEIQVFHQLEVLDLYGCGLGDSHDIFRHLSSEAFTRLVRLFLGDNCLTDKGLQRLTAPIRVMKKGLENLQQLDLSGNPLSEKGLWYLTCFKKLEELDVSRTSVKLDASLKSFFRAMMRMELSAEPLKVFTHAKCQTEGWAEEVINQWERKASELPNKEPKPRTNALQFYGREKFVRERIHSTSNDRRTEGQMTIIHFYKPHSPTQQTGHSKDTTAALTVLMGRKRKLSGEKEEQAESSPPAKRPPESTFSAEDLNLLNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0Y0</accession>
    <name>A0A3B4C0Y0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ASB17</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0Y0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005587"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005044.2">
        <property type="protein sequence ID" value="ENSPNAP00000005587.1"/>
        <property type="gene ID" value="ENSPNAG00000001913.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432459"/>
    <dbReference type="GeneTree" id="ENSGT00390000018077"/>
    <dbReference type="OrthoDB" id="6419934at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03716">
        <property type="entry name" value="SOCS_ASB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039147">
        <property type="entry name" value="ASB17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20966">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20966:SF2">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="172"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="231" mass="26259" checksum="8FF4E4CC850FFDB7" modified="2018-12-05" version="1">MSRRAHVLLHLVARVMIRARAHIPRSRADLQSFLLDFIHGASPHSAACVRRRTLREDAVRVLLERSAHTGLTITASPGIVGHITPLAFLAQWRCSRVLKQLFQCGHLERESRPSNVLLSILFSPPRLEEAPDKRSHTQVVKDIEDCMALCFRVLTHISIADIELQVIHGCSPLLEDWRVFIPPSRYQQPCELTHLCRVAIRRTLMLTGGLPDSIKTLPLPTYLKNYLNLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7S3</accession>
    <name>A0A3B4C7S3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">HERC2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006489"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003475.2">
        <property type="protein sequence ID" value="ENSPNAP00000006489.1"/>
        <property type="gene ID" value="ENSPNAG00000012669.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154975"/>
    <dbReference type="OMA" id="WRNHGST"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd08664">
        <property type="entry name" value="APC10-HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14402">
        <property type="entry name" value="UBA_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02344">
        <property type="entry name" value="ZZ_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000006">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000074">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000033">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000010">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000006">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000006">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006624">
        <property type="entry name" value="Beta-propeller_rpt_TECPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021097">
        <property type="entry name" value="CPH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
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<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Pygocentrus nattereri</name>
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            <taxon>Neopterygii</taxon>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
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                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <property type="entry name" value="AMIGO"/>
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    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24368">
        <property type="entry name" value="AMPHOTERIN-INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24368:SF62">
        <property type="entry name" value="AMPHOTERIN-INDUCED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043904620" description="Ig-like domain-containing protein" evidence="14">
        <location>
            <begin position="27"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="261"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008042.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="492" mass="55495" checksum="8584EF00918EEBA0" modified="2024-11-27" version="2">MWFHTRGMTSPALLVLFSFWLGFSEANCPSGCLCPSDIVNCGSLGLDRFPPVLPPTTSILDLSHNRLTWIAAGSFHGLPRLDVLRMSHNQISLLSPAAFHNATTLRHLDLSSNRLQVVAKHHFQDLLGLEELLLYNNRITRIESNALMGLSNLRKVYLSINQITDFPFFSIRKHSHPKLAALDLSSNRMSRLPLDDIVLLPVAVQRGLFIHNNSLACDCSTYRMFWHWDKEGYEAIRDFKDKYLCRMYGEPLTSIRFLRSPRFFENCTIEKMISLISPKAEMMVYEGEQVRLDCTGTLSSVVISYSWVIPHQENLTLLIQNGTLRLNQDGSLEILAVQSADSGIYQCIAVDTVRMINESREVNLTVVPQRLSEESFSTGYTTLLGCIITLVLILMYLYLTPCRCGCCKPPPPSPTISGLGEDRCTLASIFGAPPSAELKQKSKSNPERHVVFLEPLMQDKNGHLTAALMEEEPTGQWDMPQLNRIKEFDENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9U7</accession>
    <name>A0A3B4C9U7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-hydroxytryptamine receptor 1B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Serotonin receptor 1B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">HTR1B</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Homodimer. Heterodimer with HTR1D.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9U7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008393"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000226.2">
        <property type="protein sequence ID" value="ENSPNAP00000008393.2"/>
        <property type="gene ID" value="ENSPNAG00000013992.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222287"/>
    <dbReference type="OMA" id="HIGAMIV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046849">
        <property type="term" value="P:bone remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050795">
        <property type="term" value="P:regulation of behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002147">
        <property type="entry name" value="5HT1B_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF201">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00513">
        <property type="entry name" value="5HT1BRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0085">Behavior</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 18" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 18" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="20">
        <location>
            <begin position="37"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008393.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="362" mass="39963" checksum="D1959E5EA15D197E" modified="2024-11-27" version="2">MTRKRRRGQNASGAAPALVQALVATLLSLLTCATALSNAFVVATIARSRKLHTPANVLIGSLAFTDLLVSVLVMPVSIVYTVRRVWTLGRVLCDLWLSSDVTCCTASILHLCVIALDRYWAITDAVGYSKRRTISRAATMIAAAWVIAVSISLPPFFWQQVRAEDEVTTCTVNTDHVFYTIYSTFGAFYIPTLLLIALYGRIYAEARKRILKQSPKRVGKRLTSARLISGSPGSVASTTSLNYGTHEASSSDAMPVANNQVKVTVSDALLEKKRISAARERKATKTLGVILGAYIMCWLPFFIYTLLVPICTTCTFYPELFDVFTWLGYLNSLINPIIYTMSNEDFKQAFHKPSLFIKASIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9Y5</accession>
    <name>A0A3B4C9Y5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">KAT6B</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017552040.2">
        <property type="nucleotide sequence ID" value="XM_017696551.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017552041.2">
        <property type="nucleotide sequence ID" value="XM_017696552.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037394212.1">
        <property type="nucleotide sequence ID" value="XM_037538315.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000008752"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035042.2">
        <property type="protein sequence ID" value="ENSPNAP00000008752.2"/>
        <property type="gene ID" value="ENSPNAG00000003109.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426805"/>
    <dbReference type="GeneTree" id="ENSGT00940000157372"/>
    <dbReference type="OMA" id="AFQHQSG"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070776">
        <property type="term" value="C:MOZ/MORF histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010484">
        <property type="term" value="F:histone H3 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15527">
        <property type="entry name" value="PHD2_KAT6A_6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000123">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000132">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000035">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048589">
        <property type="entry name" value="SAMD1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
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    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF73">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21524">
        <property type="entry name" value="SAMD1_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="19" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="13" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAMD1-like winged helix (WH)" evidence="27">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
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    <feature type="domain" description="H15" evidence="25">
        <location>
            <begin position="103"/>
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    <feature type="domain" description="PHD-type" evidence="24">
        <location>
            <begin position="213"/>
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        </location>
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    <feature type="domain" description="PHD-type" evidence="24">
        <location>
            <begin position="269"/>
            <end position="320"/>
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    </feature>
    <feature type="domain" description="MYST-type HAT" evidence="26">
        <location>
            <begin position="716"/>
            <end position="990"/>
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    </feature>
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="372"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="433"/>
            <end position="537"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
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            <end position="589"/>
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    <feature type="region of interest" description="Disordered" evidence="23">
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            <end position="661"/>
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    <feature type="region of interest" description="Disordered" evidence="23">
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            <end position="1050"/>
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    <feature type="region of interest" description="Disordered" evidence="23">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
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            <begin position="1532"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
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        </location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
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    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS52014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008752.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2083" mass="230620" checksum="4A70A4999C80B85B" modified="2024-11-27" version="2">MVKLANPLYTEWILEAIQKIKRQKQRPSEERICHAVSTSHGLDKGVVLQQLELAVKDGSVLKVTSKGCASYKDPDNPGRIAAIKHGGPASSVVGSVLPAGDLRHVDWNKVLRRAIEGLGVPAGSSLRNIERFLRSQDDLVNVVANPRFQQRLRLAAKRAVNNGRLLKEGPLYRIKNAGAGGKAGFRSSVALALDLPPVTLLPHEKDQPRADPIPICSFCLGTKESNRDKRPEELLSCADCGSSGHPSCLKFSADLTANVKALRWQCIECKTCSSCQIQGKNADEMLFCDSCDRGFHMECCDPPLSRMPKGMWICQVCRPKEQEEKRLLHRTAAQIKRRYAKPGRPRKNLSHPTTLDSGDAGLLELSEREEHGRGLKSTICTPPSSSTIAPTTDARSRLSLTDPLTSTPASLTALTMLPPYNKKTKSLIDGLSKFFTPSPLGRRMRGEASDSSEQRRPKKRGYRKRLCNPHSSTTSTGTSQRLTAPASAISAPCPLSGHSPASLNPSQSPSPPSAQSPQSSSSQSSDPSLSSLPSSSQLKGLFDGLSHIYATQGQSRQKGLPSYAPPKRGRGTRDASSSPKTPLQLHGNKSIKGFNSKLASVSCSSSGWAPKRGRPFKTALHFKRTPFLKKHRLLGKFRFKASPQRGNPTPGRGSLADGRIKPDQNHVLNTLRCLKREAQEGGLPSPNDQVSEDDLNMFRQAQELSAKRTELVSSSDQGQYPSLIEFGKYEIQTWYSSPYPPEYSRLQKLYLCEFCLKYMKSRNILQRHSKKCGWFHPPANEIYRKDNLSVFEVDGNVSKIFCQNLCLLAKLFLDHKTLYYDVEPFLFYVLTQNDEKGCHLVGYFSKEKLCQQKYNVSCIMIMPQYQRQGFGRFLIDFSYLLSRQEGQAGSPEKPLSDLGRLSYLAYWKSVILEYLHNHPDKSVSIKGMSKATGMCPHDIATTLQQLNMIDVQNGRFVIVRKRHLIQRHMERLREKPRLCEVDPDCLVWTPGGSACRNPSGGWQRSRPHPPEETGDLADRNASATEKAAGMMFSRRKPPLTKCKNYSRRPPEPLFKSIVEKQLEASHDSSSDDDDDASVQSQSMTGLKRKRAGTLRKKRGRRRVRINSSVTTETISERTEVLDEPFENSDVESPAPQLEHSIRREDSDEDEKKNLRAVVRRPGRPRKRERPDDNHSDPSKEEKVEGIIDAVKKDNPRPVKRKKGWPKGVKRGPPKWRLKKERKMGFKLNLYTPPETPMVSVDQEPDEEQVASPVSVPEDCEPCREPEPVKAEQNTLVSENLRPEPQEPTEEHEDDEHLDKPDSVDTSCGAESNEAPIGPVNFAEQSTENTEEGGNQEPDEEEEHECTASQDPEERDDEEEEEEEPEPTHVEDHDADDEDDGHVDSSEPRKEDPVAVPDDKEPLDDTEPTPEPCNDKKEQDLPEECAGEVASDSGLVNMDAQDNTRDSDHHPDSGSEEDESSQNVPQKEQDAGPLTPAIKEDHSICPEIDMETAQAVQSLTQEAEQERHFQDCVETQEACRSLQRYVHVDQSPQMTPVDDCQQSDHSSPLSSVHSHPSQSVRSVNSPAVSILESSYTQISPEQGAISVHSLHNNMETSPMMDVPSVSDHSQQVVDSGFSDLGSIESTTENYENPSSYDSTLGSSICGTGASAGASSQSSCSYGNLSSGGSHGQSSCAVSQQMAGPVVNNTGACSMLQQNSMSSPQGCSVKSPQGCVPERQPSHGHGHGHGHAHGHGHGHGHSHHHNHHHHHPHQHQHQHHQHQHQHQHHQQPLSHCSMPASFPPPMQLPDIPESNNPSTNLGLYERMGQGEYGGGHYGQPSATFSLAKLQQLTNTLIDHPLPFSHSGSHPITSYANNNGSLSSQLGPHNPTLVSLSQPPHTVTGGGPQTQATMTPPPNMTPPPMMLQRNMATPNMNLPPSQRLQTQMTPKNHHHHPVSIRSKSAPLPPHHQQQMYGRSPQTVTMQAPSRTLAMPPRMNMGVNLMPASAYNVNSMNVNVNMAPLNAMNTYRVTQPMMNGGYHSNPPYMNQTPQYSMQMGMMGTQPYPQQPMQAPPHGNMMYSPAGHHGYVNTGMSKQPLNGPYMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEH6</accession>
    <name>A0A3B4CEH6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PRKR interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PRKRIP1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRKRIP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555063.1">
        <property type="nucleotide sequence ID" value="XM_017699574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEH6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010352"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016985.2">
        <property type="protein sequence ID" value="ENSPNAP00000010352.1"/>
        <property type="gene ID" value="ENSPNAG00000015892.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428520"/>
    <dbReference type="CTD" id="79706"/>
    <dbReference type="GeneTree" id="ENSGT00390000005003"/>
    <dbReference type="OMA" id="ETPSFIM"/>
    <dbReference type="OrthoDB" id="10067079at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009548">
        <property type="entry name" value="Prkrip1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507:SF0">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06658">
        <property type="entry name" value="DUF1168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="117"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21175" checksum="26B2BF599E117C9C" modified="2018-12-05" version="1">MAAPQKDAKPVKTGGKEPQPLIIAKSPAEEQRLKLERLMRNPDKPVPIPERPKEWTPRAPPEFVRDVMGSSAGAGSGEFHVYRHLRRREYQRQDFLDRLSEKQNMDEEYLMKIEENQKAAEARTSKRRKKREKLKLKKQMAKKAKVESKKESDKEESSASDNDSEEREAEDDAEVPSFVMGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGA9</accession>
    <name>A0A3B4CGA9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger protein SNAI2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Protein snail homolog 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SNAI2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578769.1">
        <property type="nucleotide sequence ID" value="XM_017723280.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGA9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010360"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035679.2">
        <property type="protein sequence ID" value="ENSPNAP00000010360.1"/>
        <property type="gene ID" value="ENSPNAG00000015896.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442954"/>
    <dbReference type="CTD" id="6591"/>
    <dbReference type="GeneTree" id="ENSGT00940000154681"/>
    <dbReference type="OMA" id="HFNSAKK"/>
    <dbReference type="OrthoDB" id="5428132at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000085">
        <property type="entry name" value="Snail zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000942">
        <property type="entry name" value="Snail zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000207">
        <property type="entry name" value="zinc finger protein SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001114">
        <property type="entry name" value="Zinc finger protein SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF42">
        <property type="entry name" value="ZINC FINGER PROTEIN SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="258" mass="28622" checksum="9050FC0CB842ECE0" modified="2018-12-05" version="1">MPRSFLVKKHFNSAKKPNYSELESPTVFLSPYLLKSIPVPVIPQPEVLSPVAYNPITVWTTSDSLLSPLPSDLSPVSGSLSPLSDASSKGRAGSESPRSGDEDERASAKLGVEDTEKFQCGLCAKSYSTYSGLMKHKQLHCDAQARKSFSCKYCEKEYVSLGALKMHIRTHTLPCVCKMCGKAFSRPWLLQGHIRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGCCVAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJ29</accession>
    <name>A0A3B4CJ29_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ZC3H18</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017546471.1">
        <property type="nucleotide sequence ID" value="XM_017690982.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJ29"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011408"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018372.2">
        <property type="protein sequence ID" value="ENSPNAP00000011408.2"/>
        <property type="gene ID" value="ENSPNAG00000017048.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423564"/>
    <dbReference type="CTD" id="124245"/>
    <dbReference type="GeneTree" id="ENSGT00730000111190"/>
    <dbReference type="OMA" id="PYRNYRR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052647">
        <property type="entry name" value="Zinc_finger_CCCH-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46582">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46582:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="504"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
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            <begin position="45"/>
            <end position="61"/>
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    </feature>
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            <begin position="79"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
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            <begin position="201"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
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            <begin position="231"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
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            <begin position="258"/>
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    </feature>
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            <begin position="511"/>
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            <begin position="604"/>
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="899"/>
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    </feature>
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        <location>
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            <begin position="1004"/>
            <end position="1021"/>
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        <location>
            <begin position="1080"/>
            <end position="1091"/>
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    </feature>
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        <location>
            <begin position="1095"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011408.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1139" mass="128773" checksum="03D1DF72D8B3C7EA" modified="2024-11-27" version="2">MDTPESPTQSPQSPEEEEKGLSDSELLESPESPAEENNAISDSELLNDEEGGDSDFDDEDRFEAGGRDPEEISDQEDDKMDRDSDREMHQQERAGTMLEDRSHMSRTPEHEEEEILETPRSPDPDSPEPEQEQPFTPAGEEQEDPRQEVEEEEDRGEAATGLGMSRPELEEDDDDERRRRRVMVVSDLKDESSSVSRELDEHELDYDEEVPEEPNPSVPEEEDTEKGPGTEDGEADEEEKDDAEKRKKSERNPILPPDQKDSQPKKPEDPRGQERGRRDSFRDKKKEEDDGEIDEGEIDDDDLEEGEVKDPMDRKIRPRPICRFFMKGNCTWGMICRFIHPGVNDKGNYSLISKPDPFSPNGAPPGGGGPHPLMPSNPWGGPTIEELPPPPLPVEPPVESAWERGLRHAKEVLKKATIRKEQEPDFEEKRFNVTIGEDEWEFDKENDFFRDRGYRITREIRDPGEVEFRDPLYGDPYADPYYDYEMEAFWRGGQYENFRVQYTETPLPYHYSDRERERDPRERHRERERERERDHRERERRQREREREREREREKERQRRKEEWERDRQKRDEKEGRPRERPLREPREKKEEKEKPLKPRSPTAMPPRGPMEPPPKKEMVPMVKRPDEWKDPWRRSKSPRRRPGLMGSPPRGRRRHRPSGSSVSLSNSSRSSSRSSSYTGSGSSRSRSRSRSHSRSSSLSSYSSHSSQRSSFSGSRSRSRSYSSSPSPTPSAQRNANKNKAEQPPGHLLKPGTLPPPRREKGPPKKIPSPSLPGQLNKPPKTFTEGSKPPNPREGGRPPPPRELGKPTNMREGRRKERHPQNPRRRTLSGSVSGSGSSYSGSSSRSRSRSSSASLSRSRSGSRKSRSLSVSSVSSVSSASSSSSSVRSADSDDMYADLASPVSSASSHSPTAGHARKERGPPRDRPTARDRDPDTDRGKLPKKDETFKDERKRVDTSDLPPKADSGTSRSGPGNRGHALNAPSVGPPGAYGSHKDIKLTLLNKQADRSNRKRYFPADKERPTSPLSKRIALSPDRGRDRRMPGRPPLSPRMDHSRGQGPRPMPTQGERKRPLSPPTKSSGKGPAASTGKPAAPGPGSGSGSGSGSGKPSSTLSRREELLKQLKAVEDAIARKRAKIPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CLQ2</accession>
    <name>A0A3B4CLQ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial fission regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MTFR2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role in mitochondrial aerobic respiration. Regulates mitochondrial organization and fission.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MTFR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537264.1">
        <property type="nucleotide sequence ID" value="XM_017681775.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537265.1">
        <property type="nucleotide sequence ID" value="XM_017681776.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537266.1">
        <property type="nucleotide sequence ID" value="XM_017681777.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537267.1">
        <property type="nucleotide sequence ID" value="XM_017681778.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CLQ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012952"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036724.2">
        <property type="protein sequence ID" value="ENSPNAP00000012952.2"/>
        <property type="gene ID" value="ENSPNAG00000005333.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410614"/>
    <dbReference type="CTD" id="113115"/>
    <dbReference type="GeneTree" id="ENSGT00950000183215"/>
    <dbReference type="OMA" id="VCQKKEY"/>
    <dbReference type="OrthoDB" id="2133332at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007972">
        <property type="entry name" value="Mtfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215:SF2">
        <property type="entry name" value="MITOCHONDRIAL FISSION REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215">
        <property type="entry name" value="PROTEIN OF UNKNOWN FUNCTION DUF729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05308">
        <property type="entry name" value="Mito_fiss_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="303"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012952.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="346" mass="37658" checksum="5821FDAFFF1AC331" modified="2024-11-27" version="2">MSLLEDLVDLLRYVLEYFGVPTDMLVPVWENTLCGQYRSIVRMIGTNLPLAPNPRVHFQIPLQTFRRHGHIDVTVDAPGIPSLADVLYLAEDEGDSCTKFRNTIPLRKCEWPQRAERPASVAPSAQNGQRALGRSTQPEALQKISALEAELMKLRAQIALIVATPTADSPLLSAPATPCLAPLPAPVLTSTPLCAPPPPPPPPPPPPPPPPPPAVAGSSQVLVTQVIRQRRAARKDKAEPSECTSTAALPSMLDILKDMNQVKLRAVERSPGGTPVRKRRSKGAVCMSDPAALIAEALKKKFAHKQKDDSFDKENRSAELSPFSSPDTPRVRVSHPLRRGQGRIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMG1</accession>
    <name>A0A3B4CMG1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017565671.1">
        <property type="nucleotide sequence ID" value="XM_017710182.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017565679.1">
        <property type="nucleotide sequence ID" value="XM_017710190.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017565688.1">
        <property type="nucleotide sequence ID" value="XM_017710199.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017565696.1">
        <property type="nucleotide sequence ID" value="XM_017710207.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMG1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012006"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019145.2">
        <property type="protein sequence ID" value="ENSPNAP00000012006.1"/>
        <property type="gene ID" value="ENSPNAG00000017730.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434789"/>
    <dbReference type="CTD" id="100330315"/>
    <dbReference type="GeneTree" id="ENSGT00530000063631"/>
    <dbReference type="OMA" id="PYCTFNG"/>
    <dbReference type="OrthoDB" id="8891572at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009950">
        <property type="term" value="P:dorsal/ventral axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000199">
        <property type="entry name" value="Activity-dependent neuroprotector homeobox b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038861">
        <property type="entry name" value="ADNP/ADNP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045762">
        <property type="entry name" value="ADNP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15740:SF1">
        <property type="entry name" value="ACTIVITY-DEPENDENT NEUROPROTECTOR HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15740">
        <property type="entry name" value="NEUROPROTECTIVE PEPTIDE-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19627">
        <property type="entry name" value="ADNP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="782"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="881"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="933"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="952"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="994"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1030" mass="113304" checksum="AD1EE6082EBCED78" modified="2018-12-05" version="1">MFQLPVNNLGSLRKARKNVKKVLGDIGLEFCKEHIDDYKDFVPNEFYIKNTTWDDVCMWDPSLTKTQEYRSKPFCCSGCPFSSKFFSAYKSHFRNVHSEDFENRILLNCPYCTYNGNKKTLETHIRLFHMPNNVTRQSTGGLQGTGTSLKDGVRLDKARDSVEQAVYYCKKCTYRDPLYNVVRKHIYREHFQHVAAPYMVKPGEKTATPNGSAAGSDTGTGNTTDSNSTAGAGNSSSTAIHCKRCLFVPRTYEALVQHVIEDHERIGYQVTAMIGHTNVVVPRPKPGIMVSPKAQVEKNVIGVTPKGTLVTAGVRPLSAQQLSRIVIPKGSLNSSGVLSGVHLKPGTISLKAGTTQAVSIGGQQVRITLPGNAQLSVPQQSQTARPHLSASHLRSAVSASTSTSVLKIPSLTSRVQAAAANVSSAPPKKGASVLGTSYTQKWKICTICNELFPENVYSSHFEKEHKAEKVPAVANYIMKIHNFTSKCLYCNRYLPSDTLLNHMLIHGLSCPHCRATFNDVEKMVAHMRASHPNESVGVRTDSPLTFDLTLQQGNPKNVQLIVTTYNMRDAPEESVAFHAQNNASPIPQGNKPATSHIPKIHNEAPDGTHARSAPQAAVPYKRDVGKTLCPLCFSILKGPISDALAHHLRERHQVIQTVHPVEKKLTYKCIHCLGVYTSNMTASTITLHLVHCRGVSKTQNGQDSRPAPTTRVVKAQGASLKRAGFQHDEEADLKKRKIGPGNQARPSLPASVELKEEPVVLALDPKGHENESYEARKAFLTKYFNRQPYPTRREVEKLASCLWLWKSDISSHFANRRRKCLRDCETQKANVLLGFNMHDLAQLQHPMDFDSKWFFEGHIQDQESRTSRDFIARSASAIIRFHENTTTNGDMQETEEARSTPKASPHSNGEKDTLGNNCNESVKIIVKPQGVLNSEPISVDSDSEDENVKAAQENTDSKVSNQLASGVGIEMESKGGASDSEPEDALSGENGYGSKEELHTSERPSEEGQTGIAAKLGQSGAGTCLGEQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMY5</accession>
    <name>A0A3B4CMY5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Histone-lysine N-methyltransferase NSD3</fullName>
            <ecNumber evidence="22">2.1.1.370</ecNumber>
            <ecNumber evidence="23">2.1.1.371</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Nuclear SET domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">NSD3</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K4me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-lysyl(27)-[histone H3] + 2 S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(27)-[histone H3] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:64452"/>
            <dbReference type="Rhea" id="RHEA-COMP:15539"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.371"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-lysyl(4)-[histone H3] + 2 S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:64448"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.370"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with BRD4. Interacts (via KIKL motif) with BRD3 (via NET domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.370" evidence="22"/>
    <dbReference type="EC" id="2.1.1.371" evidence="23"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012181"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019394.2">
        <property type="protein sequence ID" value="ENSPNAP00000012181.2"/>
        <property type="gene ID" value="ENSPNAG00000018015.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155355"/>
    <dbReference type="OMA" id="DAGWPTY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140952">
        <property type="term" value="F:histone H3K27 dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140946">
        <property type="term" value="F:histone H3K4 dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="SET domain"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="AWS_dom"/>
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        <property type="entry name" value="C5HCH"/>
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        <property type="entry name" value="NSD_PHD"/>
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        <property type="entry name" value="NSD_PHD-1st"/>
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        <property type="entry name" value="PHD5_NSD3"/>
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        <property type="entry name" value="PHDvar_NSD"/>
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        <property type="entry name" value="PWWP_dom"/>
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    <dbReference type="InterPro" id="IPR047451">
        <property type="entry name" value="PWWP_NSD3_rpt1"/>
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        <property type="entry name" value="SET2_Histone-Lys_MeTrsfase"/>
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        <property type="entry name" value="SET_dom_sf"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_PHD"/>
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    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="SET DOMAIN PROTEINS"/>
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    <dbReference type="SUPFAM" id="SSF57903">
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15 27" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 26" id="KW-0863">Zinc-finger</keyword>
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            <end position="425"/>
        </location>
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            <end position="852"/>
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            <end position="1021"/>
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        </location>
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            <end position="1130"/>
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            <begin position="152"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1529" mass="170974" checksum="25B519DC88DCF19B" modified="2024-11-27" version="2">MDFSFSFMQGIMGNTIQQPPQLIDSANIRQDDVYDAISDPGDEGGPPTYESPLESGFSYPPEDPPVVANGYPTGIGSYEHQAKFALYSQYPNGSANGYGAIRNYGDHGLLLGEGTVLRTPVVQEKPSSHISSPPAPSHSHLHHQPHPPPPPHPHHQHQHQQQHQHHLPAPNPPLLPQHHHHPHPPPSSHLLPPVFTPPPPILLPTSPPPPPQSIQDGAATSNPLAHTIAPKKTEKSSPEIKIKIIKTYQNGRELFESSLCGDLLQEFQATQASRRRHEQKKEKRKKRSGRHAPTAELPEQASEEKTDQTPGPAQPSEGHPAAQEQQAAAGLKNPKAEPKEHKVEKHFPSVITSTGVCVEYEIGDLVWAKVGTYPWWPCMVSSDPQLKVHTRINTRGHREYHVQFFGSVAERAWIHEKRIVVYKGENQFEELQAETLRKTTNPTERQKLLKPQSQRERAQWEVGVGHAEAALLMTREERNENYTFIYIDKQPCDEPEPVTSPTTKTRKTARPERRLRRSRSTRKEVLPRRQQPRRQCSITSTEEHSSPNRDEEDTDLSPAGQPPSPEESQPSPRKPSPPPVRTPWRTAAARKLLPLSITMKKLNVEIIKCDWQLPKVKVEPPPKQVEREERARDQAQSPEGSSDKAEAETCSSEEERAASPTTWSDPESGEHASKEAMERKQQRRCVRSRSESEKSVEPIPKKKVKKEQAETAPQMDFKTGSQKGASEISDSCKPLKKRSRASTDVEMASSLYRDTSDSDSRGLNDPQSGFGKRSDSPATVDADGSDAQSVDSNVSRQGSSSSKKDLVCQVCETFGDSLLSCEGDCNRLFHPECLASAGGMEGEAICTECRTGSHPCFSCKVAEGDIKRCSVNGCGRYYHETCARKYTGSTAEPRGLRCPQHSCATCCLERDLHKANKGRMMRCIRCPVAYHTGDGCIAAGSVVITPHVIICSNHSSARRNGHLSSPVNVGWCFICARATKKNIGKGGRLLCCEACPASFHPSCLNIEMPEGVWLCSECRSGKKPHYKQIVWVKLGNYRWWPAEICNPRLVPSNIQSLKHDIGDFPVFFFGSHDYYWIHQGRVFPYVESDKNFAEGQVGINKTFKKALEEAARRFQELKAQRETKEALEQERNSRRPPPYKLIKFNKPVGKVQVHVADLSEIPRCNCRPTDERPCSQDSQCLNRMLQYECHPQVCPAGERCLNQSFSKRIYADTEVIKTSARGWGLKTKQDLKKGDFVMEYVGELIDLEECRQRISHANKNHVTNFYMLTLTKDRVIDAGPKGNLSRFMNHSCSPNCETQKWTVNGDIRIGLFTLCDIAADTELTFNYNLDCLGNGRTSCHCGAENCSGFLGVRPKSAVVMEKEEKVKNSKLKAKKRKLKLESRQTHEYYCYCCGEGGELVMCDKKDCPKAYHLLCLNLTKPPYGRWECPWHQCNLCQRPASAFCHFCPTSFCREHEAGQLALSAQDSRPCCPKHDPAQTSRARVKKEQPEPGEEDGEGEGEEEEEEEEEEGVAGGGGKNGERGGEAEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CP62</accession>
    <name>A0A3B4CP62_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037392683.1">
        <property type="nucleotide sequence ID" value="XM_037536786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CP62"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013837"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021535.2">
        <property type="protein sequence ID" value="ENSPNAP00000013837.2"/>
        <property type="gene ID" value="ENSPNAG00000019717.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412170"/>
    <dbReference type="GeneTree" id="ENSGT00390000008061"/>
    <dbReference type="OMA" id="QLANICQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33488:SF2">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33488">
        <property type="entry name" value="ZGC:162509"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013837.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="685" mass="78077" checksum="F353F5E862D15A41" modified="2024-11-27" version="2">MDSKIAKTCQSLTTAEEMRNQTKLVMQPYANWEEYLTPAPLSIAILGELVFISSNTDFSINKNPPKDGYKHIKYPDSFRACLMQVCNSGWWAFNEAHKNMDQIRLHTMAVPDYMKTAVKILFQGNDEIVEAHLPDQLENISVIANDCLALAESTEKRFTDVIIIIQELLEACINAEHFYGEELEEIKKKLEENKIRKKSSEEAAKRSKKAVKFMKKQLKEAQATYQKAMDSLPDGWNMLAMEFAGAMAESFTSVVTGMTAIVAQPVVPILNVSKTITVAAVKDTNSPADGVDEMNIYSKSAEILTWTVNIQQYVRSNNIDWKDLYDQKNKSTKTDFVKNQFERINSSLNSFPECKPKQHAKDLCEEAINICEELAKYAPDGKCEDKKTNELHERIRKLIESARTFDCKSKNITKTPSLIPKPPMLSKEGSKMQRAFDKGTENVRFSVEQSRAQLDNTRELYDKSVENMEKNQKELTEILITMRNCEVKEIDFNTTIKMLIKGMDAMGKVKEQWEKMSRFFQMVSNIVKVSLTTTLKDFVSTSTKTQSLSYNSKLFSKDLLYSQAFQATNIASLVHMISATYTEVSDKYLMDRVSSLGKLMAMDKTKSEFQKERLQLQNSCDKAQKDILRLVLKNKAEFERNSDSRIKKIDKELLAILPSAAPEEMKSIQAAVENGFTKEEEADYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRH7</accession>
    <name>A0A3B4CRH7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">aromatase</fullName>
            <ecNumber evidence="17">1.14.14.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Cytochrome P-450AROM</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Estrogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">TLR3</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the formation of aromatic C18 estrogens from C19 androgens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>androst-4-ene-3,17-dione + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = estrone + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:38195"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:17263"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>testosterone + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = 17beta-estradiol + formate + 3 oxidized [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:38191"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16469"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.14" evidence="17"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013159"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020663.2">
        <property type="protein sequence ID" value="ENSPNAP00000013159.1"/>
        <property type="gene ID" value="ENSPNAG00000019022.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166529"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070330">
        <property type="term" value="F:aromatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000137">
        <property type="entry name" value="Toll-like receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041015">
        <property type="entry name" value="TLR3_TMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF193">
        <property type="entry name" value="CYTOCHROME P450 4V2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17968">
        <property type="entry name" value="Tlr3_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 22" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0391">Immunity</keyword>
    <keyword evidence="15" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="12 22" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 23" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017472478" description="aromatase" evidence="23">
        <location>
            <begin position="27"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="24">
        <location>
            <begin position="760"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="22">
        <location>
            <position position="1260"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1315" mass="150624" checksum="FF4A5EB92972D857" modified="2018-12-05" version="1">MVLRSSALSLLLCVGLLRSCPGSASPRRTGCTVERRRADCSHLKLSDVPPDLPRNISCLDMSHNRLTTLDPTALGRYPDLERLDVSYNSITALARGLCDSLPHLRRLSVQRNEVHQLTQSDLQNCSGLTHLDLSDNRLKLRGEPFSVLKNLTWLDVSRNKLTSASLGSRPQLPSLVTLILSGNDFTTLKKDDFSFLSSSSSFRVLGLSSLPLKKVESGLFKPIAGLRDLVLDGCNLSPQFLNKLCVELSHMPLRNLSLRNTQQAMLMINTFQGLITTNLTTLDLSNNKIAGIAEGSFQWLPLLESLSLENNNIKSLTNGTFLGLKSLIKLNLQKALIKTKTSHYPTIDDFSFQPLVKLEYLFMKEACFSKISENMFYGLKSLRKLDLSWSKTGLKTVDNTTFASLRGSPFLQTLNLTGTGITKLEPGAFSRLGSLTTLVLRYNFISQELTGEEFQGLNSVEEIYLSTNQQKISLTPASFIHVPTLRILTLNRALTGTLYMEPSPFRPLTNLTVLDLSNNNIANINRGLLEGLHNLKVLRMQHNNLARVWKNAYPGGPVLFLRDTQNLSILELDYNGLDEIPLRALQGLFQLKELSLSGNLLNFLQSSIFDDLQSLRYLRMQKNLLTSVPMETFGVPLSNLTELRMEHNPFDCTCDSIQWFSEWLNTTNTSIPGRAQGYICNTPPAYFNRSVIYFETDSCKNLTLFQALYVLSSTLVLGLMVIAFLVHFQGWRIQFYWNILINRTLGMKGSRYERLAEDRYEHDAYVIHAPDDRAWVERSLLPLEDKNLTFFLEDRNAVPGYSMVETIVDNIRRSRKIIFVVTEALLEDPWCRQFKAHHALHQLMEDSRDSVVLIFLQDVTDYRLSQSLLIRRSMLKSRCIVHWPLQKERIVAFHQKLMIALNSSNRTVLNNPVHMDKAYAYKFLHPWLGTGLLTSTGDKWRRRRKLLTPTFHFSILTEFLEVMNEQAEVLIEKLEKQVGKGPFNCFNYITLCALDIICETAMGKKIYAQSNYDSEYVRSVYRMSDIITKRQRMPWYWPDIVYNYFGEGREHDRSLKILHSFTESVINERAEYISYIESDSESDQGMKKRRAFLDMLLKAQDEDGNKLTNRDIQEEVDTFMFEGHDTTAAAMNWAVHLLGSHPDVQRKVHQELFEVFGESERPVNTEDLKKLRYLECVIKEALRLFPSVPFFARSICEDTHIGGYKVPKGTNAIIIPYALHRDPRYFADPEEFRPERFLPENSAGRHPYSYIPFSAGLRNCIGQRFALMEEKVILASILRYFDIKACQKREELRPVGELVLRPEQGIWITLQRRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWD5</accession>
    <name>A0A3B4CWD5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">KIF25</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538237.1">
        <property type="nucleotide sequence ID" value="XM_017682748.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWD5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015680"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037820.2">
        <property type="protein sequence ID" value="ENSPNAP00000015680.1"/>
        <property type="gene ID" value="ENSPNAG00000021740.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411267"/>
    <dbReference type="CTD" id="3834"/>
    <dbReference type="GeneTree" id="ENSGT00940000162166"/>
    <dbReference type="OMA" id="CEIYNNE"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF63">
        <property type="entry name" value="KINESIN FAMILY MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="171"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="445"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="264"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="590" mass="65658" checksum="75837B2449D817AD" modified="2018-12-05" version="1">MPLFINRDQIFAHQVHLLEHKLRSKEERILELETENALLHLRLAECMGKLRRDQEEEISAKRRCQHQGNVQKRTRAALAVLLSEVQDLKQDLQDVFAVYVGFATELEKQSKQLLEQVSCTAVQAYQGDDIQKLQAQVAILEHGLQEEKERCRTERMRRRLLHNTLVELRGNIRVHCRVRPILPFDCSQVPSTNGSSSSEVVVQAFSDDSVLVNCAKPGSPVMNKMFEFERVHGPEDTQDAVFEEVKPLLTSLLDGYNVCIMAYGQTGSGKTYTMIGSQPEDLTVPVHNSQQGIIPKAANELFKLISEKPAESHTVEMSVVEVYNNELLDLLAKDEEGARVGVKREVITTSTGTSEVPCLTHELVERCAEVMQLISAVLRLRAHSPTLVHRDSSRSHLIVTLTVTSKSPNALALARRLQGGQKNVHRGSQKEWWSPRCRRAASSCSSSPATSPYHSPCQSPCPSPRPSISQTPIRTKLQLVDLAGSESVGMSGVSGAALWENSCINRSLSALSDVLGALAEQRPHVPYRNSKLTHLLQDSIGGDAKLLVMLCVSPTQRFLTESLQSLGFGSRARQIQRDPPRRKNTGIKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWQ5</accession>
    <name>A0A3B4CWQ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transmembrane protein 237b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the transition zone in primary cilia. Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM237 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557695.1">
        <property type="nucleotide sequence ID" value="XM_017702206.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWQ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015795"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024081.2">
        <property type="protein sequence ID" value="ENSPNAP00000015795.1"/>
        <property type="gene ID" value="ENSPNAG00000021847.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430049"/>
    <dbReference type="CTD" id="447898"/>
    <dbReference type="GeneTree" id="ENSGT00390000005159"/>
    <dbReference type="OMA" id="WRIINLM"/>
    <dbReference type="OrthoDB" id="550113at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029409">
        <property type="entry name" value="TMEM237"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15383">
        <property type="entry name" value="TMEM237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="46772" checksum="F1B8DD8C1D1983FD" modified="2018-12-05" version="1">MDTDNEKVPSIRRRELPPISHRRGPRALPSLPSQETADEMPVLRTKKKKAKREVDTEGVEAPEATGDTGIEMGDMDSHRQSESQERLTPEPQENPPQKKKKKKKAQVIDPESDQTDLVQNGDMPDQNTDEEPTRKPKKRKLKPKVTDVQLNNELAIEDDDIITDAQPPIPQHALFSAPQGPSQPMAKVFIERGRRFQPAERIDRRKTSSGQIEQNFMDVQSMWTTRDVSMRVHRGFRVIGLFSHGFLAGYAVWNIVVLYILAGEQMSTLPNLLEQYHTLAYPAQSLFYFLLALSTVSAFDRVNLAKASVALRSLLTLDPLALASFLYFSALVLSLSQQMTSDRINLYSSPNASLWVPGPDHSALYPWIIVNLVVTLLVGLAWVLVSTSPEVDYTEDFLMAMEIEYPKPEEKDIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ49</accession>
    <name>A0A3B4CZ49_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550639.1">
        <property type="nucleotide sequence ID" value="XM_017695150.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ49"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017367"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026132.2">
        <property type="protein sequence ID" value="ENSPNAP00000017367.1"/>
        <property type="gene ID" value="ENSPNAG00000023609.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426006"/>
    <dbReference type="GeneTree" id="ENSGT01030000234518"/>
    <dbReference type="OMA" id="HHRAFTV"/>
    <dbReference type="OrthoDB" id="10021141at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15159">
        <property type="entry name" value="7tmA_EBI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF38">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="50"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="343" mass="38937" checksum="7555160DBFEE3CD8" modified="2018-12-05" version="1">MATQASVVSSSAIPSESIPNQTCNVFTYKESARVLFPTFYIIVLVISVSGNSLVLYITCQKKQKFNSTSLYLINLAISDTLFTLALPGRITYYILGFDWPFEEALCRLTAMIFYSNTYAGIAFMTCISIDRYLAMVHPRRCLRLRNVKVVRGICVLVWVIVLLETSPLLFRTMMKKWDEHRTCMEYSNLDESPKTPYVLLFACATGFCIPLGLILGCYSQINSKLSRMAKENPMTSKSGKSQRAKNVILLILLSFVVCFGPYHINIMQFMVRKLLHEPSCEEKKVFKMSLQVTVAMMNLNCCLDPVIYFFAIKTYKQRVVSLFKGYMPSLSSKSMPENSSSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ84</accession>
    <name>A0A3B4CZ84_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">LRRC8 pannexin-like TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>iodide(out) = iodide(in)</text>
            <dbReference type="Rhea" id="RHEA:66324"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>taurine(out) = taurine(in)</text>
            <dbReference type="Rhea" id="RHEA:66328"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LRRC8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539447.1">
        <property type="nucleotide sequence ID" value="XM_017683958.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017539448.1">
        <property type="nucleotide sequence ID" value="XM_017683959.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ84"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017397"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026185.2">
        <property type="protein sequence ID" value="ENSPNAP00000017397.1"/>
        <property type="gene ID" value="ENSPNAG00000023673.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412078"/>
    <dbReference type="CTD" id="327642"/>
    <dbReference type="GeneTree" id="ENSGT00940000154043"/>
    <dbReference type="OMA" id="RGIQYEL"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000053">
        <property type="entry name" value="Leucine-rich repeat-containing 8 VRAC subunit Ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000424">
        <property type="entry name" value="Volume-regulated anion channel subunit LRRC8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021040">
        <property type="entry name" value="LRRC8_Pannexin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF58">
        <property type="entry name" value="VOLUME-REGULATED ANION CHANNEL SUBUNIT LRRC8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12534">
        <property type="entry name" value="Pannexin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="LRRC8 pannexin-like TM region" evidence="18">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="794" mass="92404" checksum="EEEC75937B359D3D" modified="2018-12-05" version="1">MIPITELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVVNESCPPLNTSVRPSTEPRGIQYELDRHQYNYVDAVCYENNLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEETDPKPPGKADGFTDKKTSSISDQDVEASVPMLQRTKSRIEQGIVDRSDTGVLDKKEGEQAKALFEKVKKFRIHVEEGDIVYRLYIRQTIIKVIKFVLIISYTAYYVGHITFNVVCRVNIEQLTGYKLYYCAHPLATLFRILGWFYISLVIVYGLICMYTLCWMLGRSLKHYSFESIREESSYSDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLEKLRQRITKNSQDKLELHLFMLSGIPDTVFDLLELEVLKLELIPDVTIPPIIAQLSNLREMWLYHTPAKIEAPALAFLRENLKSLHIKFTDIKEIPLWIYSLKNLSELHLTGNLSADNNRYIVIDGLRELKRLKVLRLKSNLTKLPQVVTDVGVHLQKLSINNEGTKLMVLNSLKKMVNLTELELVRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLVCLKLWYNQIAYIPIQIGTLTNLERLYLNRNKIDKIPSQLFFCRKLRYLDLSHNNLTSIHSDIGLLQNLQYFAVTANRIETLPAELFQCKKLRTLNLGNNCLTSLPSRVGELTGLTQLELRGNRLEGLPVELAECRLLKRSGLVVEEDLFKTLPTEVKEQLWRADKEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0F2</accession>
    <name>A0A3B4D0F2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription factor AP-2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TFAP2E</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558572.1">
        <property type="nucleotide sequence ID" value="XM_017703083.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0F2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017797"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026720.2">
        <property type="protein sequence ID" value="ENSPNAP00000017797.1"/>
        <property type="gene ID" value="ENSPNAG00000024083.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430541"/>
    <dbReference type="CTD" id="339488"/>
    <dbReference type="GeneTree" id="ENSGT00950000182848"/>
    <dbReference type="OrthoDB" id="6252992at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030318">
        <property type="term" value="P:melanocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF13">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2-EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="212"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="428" mass="46391" checksum="45F1B34AB774A475" modified="2018-12-05" version="1">MLWKSRAKTDPYCIQAPERADGVSGSSPPSRLPQLSSFSAAPPLCHTPASDFQPPYFPPPYPQASLPYPQSQDASYPHLPDPYQSLSSLHAPQHLPQHLPPQQQQHQQQQQQWLSQRSRAEDGGLLSPTLRRDYASVPRLLHGLGEDGPLAMHHGVDDLQGMEEASSLGILDHSVIKKVPVPSKLNGSALSVLSLGKDGLGLGGISNPGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRCLRERLEKIGLNLPAGRRKAANVTLLTALVEGEAVHLARDFGYVCETEFPARATAEYLCRQSDPEQLPTRRSMLLATKEICKEFVDLMSQDRSPLGASRPTPCLEPGVQGSLTHFSLLTHGFGTPAICAALSAFQSYLLEAVKLLDKGEGGGKGHQEKELKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D101</accession>
    <name>A0A3B4D101_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CD83</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017569865.1">
        <property type="nucleotide sequence ID" value="XM_017714376.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D101"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016529"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025057.2">
        <property type="protein sequence ID" value="ENSPNAP00000016529.1"/>
        <property type="gene ID" value="ENSPNAG00000022687.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437339"/>
    <dbReference type="CTD" id="9308"/>
    <dbReference type="GeneTree" id="ENSGT00510000051337"/>
    <dbReference type="OMA" id="GTTRWYK"/>
    <dbReference type="OrthoDB" id="9422899at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15193">
        <property type="entry name" value="CD83 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15193:SF1">
        <property type="entry name" value="CD83 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017298642" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="32"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="218" mass="24446" checksum="320C119C0BECE6AB" modified="2018-12-05" version="1">MHYTGVLSLFLLSLAVREHSASGEHEALSVCGGDAVLRCLAATEPGVRYQSVIWYKVSEAPSHKLTGLVLKRLTQNNGTIQRYRGLMREVELLEGSQSLLLPNVTVEDAGTYLCFLSAPLGHQNQQREIHLRVYEQPKDDAGKYEESDTFYLVLAITLLIAALLMFYVSYICLRNTLISHKDKFSKDARLKTTQKMKHLIVSLESKGVVSKISAQEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D200</accession>
    <name>A0A3B4D200_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">R3H domain and coiled-coil containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D200"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017590"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038605.2">
        <property type="protein sequence ID" value="ENSPNAP00000017590.2"/>
        <property type="gene ID" value="ENSPNAG00000023875.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063711"/>
    <dbReference type="OMA" id="IFPCQAS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039884">
        <property type="entry name" value="R3HC1/R3HCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21678">
        <property type="entry name" value="GROWTH INHIBITION AND DIFFERENTIATION RELATED PROTEIN 88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21678:SF6">
        <property type="entry name" value="R3H AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017590.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19949" checksum="CF4FEF9FF5969477" modified="2024-11-27" version="2">MCPIFVCFLFAKITAHLKEGEVVIENAQNDYSSFENAWINQEEFAHVIEIYDFPAIFKTDDLLDAFADYSEGGMKIKWVDNTHALGVFSSASAAMQALSIRHPLLKTRTLSEGSKKAKGKAMRRAEFIQPVKERPRTDTAVARRMVTRALGLRGGSRGKQQQESLQNLCLPSNCFTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6I8</accession>
    <name>A0A3B4D6I8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">BMP6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551650.1">
        <property type="nucleotide sequence ID" value="XM_017696161.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6I8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019120"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028743.2">
        <property type="protein sequence ID" value="ENSPNAP00000019120.1"/>
        <property type="gene ID" value="ENSPNAG00000003948.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426564"/>
    <dbReference type="CTD" id="654"/>
    <dbReference type="GeneTree" id="ENSGT00940000158768"/>
    <dbReference type="OMA" id="ERQQPWP"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19396">
        <property type="entry name" value="TGF_beta_BMP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000003">
        <property type="entry name" value="Bone morphogenetic protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000016">
        <property type="entry name" value="Bone morphogenetic protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF137">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017466031" description="TGF-beta family profile domain-containing protein" evidence="13">
        <location>
            <begin position="23"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="295"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="283"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="423" mass="48393" checksum="E9EC608038BF407B" modified="2018-12-05" version="1">MKSHRFALAGLWWSSYCMFLVAGSGNLADFNNDLQLHRRLRTHEKREMQKEILSILGLPHRPRPHLLNGKYNSAPLFMLNLYNTINSEERNEVDGILDQYKSMFTTPGPPLATSQDNAYLNDADMVMSFVNLVEYDRELSPHRRHHKEYKFNLSQIPEGEAVTAAEFRIYKECVSSTFRNETFLLSVFQVVGEHPDRDADLFLLESRQLWAVEEGWLEFDITATSNLWVMSPHHNLGLQISVETSSGQNISPREAGLVGRDGALERQPFMVAFFKVSEVHVRTARSTGKRRQQSRNRSNHPQEAHKGPGHTDYNSSDQKMACRKHELYVSFRELGWQDWIIAPEGYAANYCDGECSFPLNAHMNATNHAIVQTLVHLMNPENVPKPCCAPTKLHAISVLYFDDNSNVILKKYKNMVVRACGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D7S1</accession>
    <name>A0A3B4D7S1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RING finger protein 207</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RNF207</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D7S1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019166"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028806.2">
        <property type="protein sequence ID" value="ENSPNAP00000019166.2"/>
        <property type="gene ID" value="ENSPNAG00000025744.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048612"/>
    <dbReference type="OMA" id="YEDSYRH"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055117">
        <property type="term" value="P:regulation of cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901207">
        <property type="term" value="P:regulation of heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19814">
        <property type="entry name" value="Bbox1_RNF207-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039320">
        <property type="entry name" value="RNF207"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22635">
        <property type="entry name" value="RING FINGER PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22635:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="36"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="102"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019166.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="641" mass="73638" checksum="9CD69FC87D286380" modified="2024-11-27" version="2">MTQELFWIPHLSIYRKADTMAGLPLFPLDDLHPLVCHMCHEQYRQPCLLDCYHIFCASCLHGRASENRLICPLCGCPSAVKGTNALPPEDRLLKFLVENSAESVVQCSNCETECKTQDAGMMYYCNTCSQPLCGPCRETTHKARMFSCHEIVSLAKRAKEGHRKCSLHDELYIMFSTEKKSMLCIKCFRDMQVESRMHCIDIETAYAQGCEILDQAVLAVKELQTSAREAILLLKAMLGEVRANVEEEESAICNLFNSMQEKLADRKRILLKAAQSQHEEKEKALKEQLSHLAALLPTLQVHLVTCSAFLSSANKYEFLDMGYQLMERLCRIVKLPHRLRPTQSSKINTDFCAEFARCLEPLLLRGQRRPGSVSGGVTVSNLVQSSWSTLCRSPSLSDMPQVPSLGRRPTSHRYICTKVLLAEGGDTPFTEHCRNYENSYQMLQTEIQKLKDQVQEMHRDLTKHHSLINTDTMGEILERSLHIDQQITSQYSSVQTLRGVFEEIWEETFQRVANEQEIYEAQLHDLLQLKQENSYLTTIARQIGPYILSIAKVKERLEPRFQEPKELEDDRTETMLKIYEDSTMTSESQHSDDDIIYSWDSNRNKRALIFQPSEIPPKNTDHRWQNKRNISEASCRKELPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8F1</accession>
    <name>A0A3B4D8F1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8F1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020632"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031353.2">
        <property type="protein sequence ID" value="ENSPNAP00000020632.2"/>
        <property type="gene ID" value="ENSPNAG00000013970.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162676"/>
    <dbReference type="OMA" id="YSFHRTV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051755">
        <property type="entry name" value="Ig-like_CS_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19971">
        <property type="entry name" value="SIGNAL-REGULATORY PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="28"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020632.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="138" mass="15026" checksum="0EBA6C98093F7263" modified="2024-11-27" version="2">MYFCVESGKKMLNFSTATFLAVTGQLNTSVLQTAVPWSVSPGESVTLQCTVLSEIRAADLRVLWFRAAAGQSFPEIIYTHQNSSSRQCEISSSTSCSLYEFSKNILDHNHTGTYYCAVAVCGKIIFGNGTSVELSKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DA05</accession>
    <name>A0A3B4DA05_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="20">HMG-CoA reductase</shortName>
            <ecNumber evidence="20">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:15991"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Endoplasmic reticulum membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DA05"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021172"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032225.2">
        <property type="protein sequence ID" value="ENSPNAP00000021172.2"/>
        <property type="gene ID" value="ENSPNAG00000028218.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155305"/>
    <dbReference type="OMA" id="DCHIAMD"/>
    <dbReference type="OrthoDB" id="310654at2759"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3270.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.770.10:FF:000002">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004816">
        <property type="entry name" value="HMG_CoA_Rdtase_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00920">
        <property type="entry name" value="2A060605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8 20" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0521">NADP</keyword>
    <keyword evidence="12 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="18" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="22">
        <location>
            <begin position="61"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="869"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="874"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021172.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="889" mass="97074" checksum="BA799A89F065FF2D" modified="2024-11-27" version="2">MLTRLFRIHGLFVASHPWEVIVATVTLTICMMSMNMFTGNDQICGWNFDCPKFEEQVLSSDIIILTITRCIAIIYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSKACALAKFALSSNSQDEVRENIARGMAVLGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGHPIWQLSHFARVLEEEEDNKPNPVTQRVKMIMSLGLVMVHAHSRWIADPASPNGTLDVPQVGISLSYHTPKRIDPDMPLWQFYLSRMISMDIEQVITLGLALFLAVKYIFFEQVEMESTLSLKVPNPSSMLTQKWSPEQCCRKEIALRPSKAEKPPTLPPVITKEERGTANYLVIKPLPTITEPAPKSTFVVGEEESLDSAAVEQPQPNIPSKPRPVDECLTILKNLEMGARYLSDAEVIILVNAKHIPGYKLEAMMESPERGVMIRRKMLCQKLPNPSALTCLPYKDYDYSQVMGTCCENVIGYMPVPVGVAGPLLLDGKQFQVPMATTEGCLVASTNRGCRAIALGGGASSRVLADGMTRGPVVRLPSACQAAEVKAWLESPEGFKCIKEAFDHTSRFARLEKLLVGLASRNLYIRFQSRTGDAMGMNMISKGTEQALARLKEEFPELQVLAVSGNYCTDKKPAAINWIEGRGKSVVCEATIPAKVVKEILKTSTEALVDVNINKNLVGSAMAGSIGGYNAHAANLVAAIYIACGQDPAQTVGSSNCITLMEASGPTGEDLYISCTMPSIELGTVGGGTNLAPQQACLQMLGVQGASEAHPGENARQLARVVCATVLAGELSLMAALAAGHLVKSHMTHNRSKLNLQETPGTRTKQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBH2</accession>
    <name>A0A3B4DBH2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptor 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GPR20</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568574.1">
        <property type="nucleotide sequence ID" value="XM_017713085.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBH2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020798"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031607.2">
        <property type="protein sequence ID" value="ENSPNAP00000020798.1"/>
        <property type="gene ID" value="ENSPNAG00000006782.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436547"/>
    <dbReference type="CTD" id="2843"/>
    <dbReference type="GeneTree" id="ENSGT01150000286998"/>
    <dbReference type="OMA" id="WRQPACA"/>
    <dbReference type="OrthoDB" id="6069656at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15163">
        <property type="entry name" value="7tmA_GPR20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000243">
        <property type="entry name" value="G protein-coupled receptor 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF7">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="78"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="368" mass="41453" checksum="F007B4835130AAF2" modified="2018-12-05" version="1">MHRHRANPRTMEMTRIEGSLSLNVTSAPPVTTNSSPPSREPYLHRLAHLDEGLYNDFYSLWIALVVINTLIFMVGMALNSLALYVFCFRTKPKTTSVIYTINLAVTDLLVNLSLPTRIILYYSGGKCLNCSYVHIFSYFVNMYCSILFLTSICVDRYLAIVQVEASRKWRNPNVAKVVCACIWLFAIIVTYSFLTTAFKHSACCVSKLFALTVFEFFLPLVIIVVFTVRIMCALSSPGLMQQSREKRMKAVQLLTTVLVIFTICFTPFHVRQVLVYFHPDLPHHVIVYHVTVTLSSLNSCLDPVVYCFVTTNFQSTMNRFFRKAEAEQTSGDIISMQKSSKGSGTVFAIANSMIMVNMSSPQPGSLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DGC7</accession>
    <name>A0A3B4DGC7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017544448.1">
        <property type="nucleotide sequence ID" value="XM_017688959.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DGC7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022156"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041030.2">
        <property type="protein sequence ID" value="ENSPNAP00000022156.1"/>
        <property type="gene ID" value="ENSPNAG00000029421.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415875"/>
    <dbReference type="CTD" id="692351"/>
    <dbReference type="GeneTree" id="ENSGT00940000158038"/>
    <dbReference type="OrthoDB" id="10069833at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01215">
        <property type="entry name" value="PTB_Dab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000035">
        <property type="entry name" value="Disabled homolog 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048559">
        <property type="entry name" value="DAB1/2_SBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048561">
        <property type="entry name" value="Dab_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47695:SF4">
        <property type="entry name" value="DISABLED HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47695">
        <property type="entry name" value="PID DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21792">
        <property type="entry name" value="DAB2_SBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
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        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="272"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="505"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="272"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="595" mass="63728" checksum="E4A3E43DBFDAA27D" modified="2018-12-05" version="1">MSTEAELQPAARPSVRKDSKRRGQDRSEAALIKRFKGDGVRYKAKLIGIDEVTAARGDKLCQDSMMKLKGIAASARSKGEHKQKVFLTVSFGGIKIFDEKSGVLQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQSAEPVILDLRDLFQLIYEIKQREEIEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDQSEESIYQVPTSQQKEGVYDVPKRHPNGHNGQTNLLDLDAELVLVPQNINQLELFGDMSTPPDITSPSTPASPANTLDPTLAHQPPSELFTPFNPASVPSGYVTMGAVPPAWAQQQFAAQTPLAFGVQSPVPMAQVLPGGQPLIWGQANLFPATQQQWAAMAGAHFSPAAFMPAQTVGPLPAAMFQTLAPMQVAAAAAASCETPIVGVAGAGAGVGPVPGKSASTASSPQHGERSLPRQAKMSKEMFKDFQMAKPPAVPTCKSEQPSLACTTDAFTSYFSRVGMAQDNDDCDDFDISQMNLTPVTSTTPSTNSPPTPAPRQSSPSKSSSQASDPPTDDSFGEVEGSPSRSGEEDAAGDAQSPCVSEPQAECGGSEPDSPQVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHA1</accession>
    <name>A0A3B4DHA1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pro-thyrotropin-releasing hormone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TRH</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561181.1">
        <property type="nucleotide sequence ID" value="XM_017705692.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHA1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022833"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014156.2">
        <property type="protein sequence ID" value="ENSPNAP00000022833.1"/>
        <property type="gene ID" value="ENSPNAG00000007396.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432093"/>
    <dbReference type="CTD" id="7200"/>
    <dbReference type="GeneTree" id="ENSGT00390000016951"/>
    <dbReference type="OMA" id="QESFTCN"/>
    <dbReference type="OrthoDB" id="9949225at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008437">
        <property type="term" value="F:thyrotropin-releasing hormone activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042755">
        <property type="term" value="P:eating behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001692">
        <property type="term" value="P:histamine metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014050">
        <property type="term" value="P:negative regulation of glutamate secretion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014054">
        <property type="term" value="P:positive regulation of gamma-aminobutyric acid secretion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032024">
        <property type="term" value="P:positive regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TRH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17530">
        <property type="entry name" value="PRO-THYROTROPIN-RELEASING HORMONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17530:SF2">
        <property type="entry name" value="PRO-THYROTROPIN-RELEASING HORMONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05438">
        <property type="entry name" value="TRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001795">
        <property type="entry name" value="TRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5 9" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017106923" description="Pro-thyrotropin-releasing hormone" evidence="9">
        <location>
            <begin position="25"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="61"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="217"/>
        </location>
    </feature>
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        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="263" mass="29766" checksum="6F2D3B60D347A7F1" modified="2018-12-05" version="1">MDMRAASLILLSSLVLCSLSGIQCQNIPAEEEPGERPSLEEILQRAHSILMRSILKKMEEEENADEMLAPQPEWLEKRQHPGKRQHPGKREEEDEEDDGDDEDYLGLQKRQHPGKREDEADLIELQRRQHPGKRWMLEQMQETPGAPNAFLSELAKRQHPGKRLVMYSKRQHPGRREADAGEPGAGDLLEMDKRQHPGKRFWDASAASDASAGGPCDLLASPSCSRASLLLELLDNVSKSRAEEKRQHPGKRLAVEEDLPELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJJ2</accession>
    <name>A0A3B4DJJ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ATP6V1G1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538343.1">
        <property type="nucleotide sequence ID" value="XM_017682854.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJJ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023623"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012163.2">
        <property type="protein sequence ID" value="ENSPNAP00000023623.1"/>
        <property type="gene ID" value="ENSPNAG00000000731.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411339"/>
    <dbReference type="CTD" id="9550"/>
    <dbReference type="GeneTree" id="ENSGT00940000154399"/>
    <dbReference type="OMA" id="ARKYRQD"/>
    <dbReference type="OrthoDB" id="250802at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097401">
        <property type="term" value="P:synaptic vesicle lumen acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2950:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="8"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="118" mass="13500" checksum="BC22BD36B4EEC43D" modified="2018-12-05" version="1">MASQSQGIQQLLQAEKRAAEKVAEARKRKNRRLKQAKEEAQAEIEQYRLQREKEFKTKEAAALGSHGNSAVEVDKETLDKMSCIQKSYQQNREAVLNNLLKMVCDIKPEIHTNYRVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJW9</accession>
    <name>A0A3B4DJW9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein regulatory complex protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">IQ domain-containing protein D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IQCD</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the nexin-dynein regulatory complex (N-DRC). Interacts with CFAP52.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DRC10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJW9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023758"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011835.2">
        <property type="protein sequence ID" value="ENSPNAP00000023758.2"/>
        <property type="gene ID" value="ENSPNAG00000000851.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111354"/>
    <dbReference type="OMA" id="AKIQKYW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042815">
        <property type="entry name" value="DRC10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31598:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31598">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="218"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="311"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="222"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023758.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="401" mass="45656" checksum="5D49C209FC731EED" modified="2024-11-27" version="2">ISPPGDELPPVALTPVSPNKLSLKIIDPSRKKLVSPEPQRIVGVLDECIRKMELVALLPSELTHPESLSVSLGGKAVGFLTEHLRVVKERRALTGDTEEEERDAATKGARNSLLNLLRLLRGCPTAGAGLKGAGPITEDRRGVQELVAGLQELRAVVLERLLTTASEERERSRHIQEVSMRHRSNLEVVATLEKEVAAAIKDKDEEISKQNEAIRRLKGSLHQKEKSSEDFVARTQKEAQRQDESDQKTSEGKQTRLQQEANQLRVQLNNLITEHKEAELALRKKKYKLETEIENWIQKYDTDMGEKQTELEEMTVIYEEEKAELRELQEHYAVLELEYSQIMEERRIAQEKKEEEEREREVKSHAVVIIQAFWRGHCVRKVMKAKVKSKKAKKGKGKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKA4</accession>
    <name>A0A3B4DKA4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysyl oxidase homolog</fullName>
            <ecNumber evidence="13">1.4.3.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-lysyl-[protein] + O2 + H2O = (S)-2-amino-6-oxohexanoyl-[protein] + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24544"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:12448"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:131803"/>
            <dbReference type="EC" id="1.4.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>lysine tyrosylquinone residue</name>
            <dbReference type="ChEBI" id="CHEBI:20489"/>
        </cofactor>
        <text evidence="13">Contains 1 lysine tyrosylquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="13">The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the lysyl oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.13" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017566718.1">
        <property type="nucleotide sequence ID" value="XM_017711229.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKA4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024812"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009186.2">
        <property type="protein sequence ID" value="ENSPNAP00000024812.1"/>
        <property type="gene ID" value="ENSPNAG00000009564.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435404"/>
    <dbReference type="CTD" id="404621"/>
    <dbReference type="GeneTree" id="ENSGT00940000154779"/>
    <dbReference type="OMA" id="PRTNERQ"/>
    <dbReference type="OrthoDB" id="547291at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004720">
        <property type="term" value="F:protein-lysine 6-oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050912">
        <property type="entry name" value="LOX-like_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001695">
        <property type="entry name" value="Lysyl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019828">
        <property type="entry name" value="Lysyl_oxidase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817">
        <property type="entry name" value="LYSYL OXIDASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45817:SF6">
        <property type="entry name" value="PROTEIN-LYSINE 6-OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01186">
        <property type="entry name" value="Lysyl_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00074">
        <property type="entry name" value="LYSYLOXIDASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00926">
        <property type="entry name" value="LYSYL_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 13" id="KW-0886">LTQ</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0765">Sulfation</keyword>
    <keyword evidence="8 13" id="KW-0801">TPQ</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017218831" description="Lysyl oxidase homolog" evidence="15">
        <location>
            <begin position="29"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="63"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="395" mass="45105" checksum="C55FB56F3B8766FF" modified="2018-12-05" version="1">MAARNFGSVFYACAHVCLLGCVLQSAHSQREPRPVGNNAALRQTIQWQHNGKLFSLLSQGSEYQPPLRRDAEGEQAEARRPVAVISRPSRVGGDPSGSSQYARGRGEARARSRSGARERATGEPRRTNGTEWENKRNESAPEDAMVGDDPYNPYKSTDPDNPYYNYYDTYERPSPRQRPGYGTRYFQYGLPDLVADPYYIQASTYVQRVPMYNLRCAAEENCLASSAYRSSVRDYDTRMLLRFPQRVKNQGTSDFLPSRPRYAWEWHSCHQHYHSMDEFSHYDLLDASSHRRVAEGHKASFCLEDTSCDYGYYRRFACTAHTQGLSPGCYDTYNADIDCQWIDITDVKPGNYILKISVNPSYQVQESDYTNNVVRCDIRYTGNYAYASGCHVSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DL58</accession>
    <name>A0A3B4DL58_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Serine/threonine-protein kinase VRK1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Vaccinia-related kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">VRK1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017566774.1">
        <property type="nucleotide sequence ID" value="XM_017711285.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DL58"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023851"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011604.2">
        <property type="protein sequence ID" value="ENSPNAP00000023851.1"/>
        <property type="gene ID" value="ENSPNAG00000000923.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435441"/>
    <dbReference type="CTD" id="7443"/>
    <dbReference type="GeneTree" id="ENSGT00940000155554"/>
    <dbReference type="OMA" id="RETHDTH"/>
    <dbReference type="OrthoDB" id="2687620at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000348">
        <property type="entry name" value="serine/threonine-protein kinase VRK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="37"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="345"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="421" mass="46794" checksum="E007400C2F23A8F7" modified="2018-12-05" version="1">MPPKARAAGKGKAPAKRKLAEEFPPGEILTDTAKKSWKLGAPIGQGGFGLLYLASDDASKAVGADAPYVVKVEPSDNGPLFSELKFYMRAAKPELIGAWIKSRKLKYLGVPKYWGSGLHEKGGKRYRFMVMDRFGTDLQKKFEQGGKRFPRKLVLQLGLKLLDILEYIHAHEYVHADIKASNLLLSHKNPNQVYLVDYGLAYRFSPDGVPKEYKEDPKRCHDGTIEFTSIDAHKGVTPSRRGDLEILGYCMIQWLCGRLPWEDKLQDPVYVRDSKLRCRDNIRDFMKSCFTTGSEPGEMAKFMEEVKSLGYTDKPDYQKLRGTLLQGLKSIGATDDDKLDFSVSVNGASPSAAKSTKRKKPVEKGASNDESDGSPAKKSRPPPKKELNGVKKAGPKKRLVEMGTQTSPGLKKSRGRPKKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DMB8</accession>
    <name>A0A3B4DMB8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FA complementation group G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FANCG</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017542812.2">
        <property type="nucleotide sequence ID" value="XM_017687323.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DMB8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025542"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007364.2">
        <property type="protein sequence ID" value="ENSPNAP00000025542.2"/>
        <property type="gene ID" value="ENSPNAG00000010666.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414453"/>
    <dbReference type="GeneTree" id="ENSGT00390000007195"/>
    <dbReference type="OMA" id="CCLAWRA"/>
    <dbReference type="OrthoDB" id="6355951at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039684">
        <property type="entry name" value="FANCG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15254:SF2">
        <property type="entry name" value="FANCONI ANEMIA GROUP G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15254">
        <property type="entry name" value="FANCONI ANEMIA GROUP G PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="235"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="460"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025542.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="630" mass="70793" checksum="648F11892CEDCA20" modified="2024-11-27" version="2">MSVTPCLVDRWTEENNNIVRKWKEYENRRQPLQDVRKHCYAEFLKLWQKIQGLPATTEQLQLEITASYNTLILSLSYSKPQDTQHHLTNSLVRALEAVGAQVSKTDLGTLLQAVLKTFGGTVYQPCIHKLLLIQWAIWLSECQFDGILHLLQQINPKNKITPSGDLLTEIRNLTFSIQEDISLLVAMAAKDLKDLLHICTFVSKGVEQMRKENYTEALLAIQEAATLSSPRALLAHIHTLTGLCFTKLGRPQSALQCYRKALEVDFSCQRALYQSSLVYRQSGNSQAEIEALHLLHSAVLLHTERDSSSTPAALISPDMLLGGEQMAFISQVPSPVLILHTLAHRCLLNDRNSESAEFYVDLLATLQSPDSRPHGPTIDALPFPRIPVVYLEAAFAVLKAKRFWDSIAICDEVIAKTVDLVPERLLLEPHHRSAGPTELAAVGVESEAVEEKLDCVLWSGAAYFLQGHAYLQLKDTKEALTIFTRAINQLVKVCVKQKCWTVSNLGQTEDSDNKVATLEALKGQTLAGRGVCFIERGQLKEALRDFQLSLQASGCRNTEMWLVETLWRLDRKKEAAAMWHQSYNSTNSPTPVELPLYLQTWQEDATNLDHSNLNEKMEKFIHSCGDITMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DPA9</accession>
    <name>A0A3B4DPA9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Potassium voltage-gated channel subfamily A member 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KCNA10</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Voltage-gated potassium ion channel that mediates K(+) permeability of excitable membranes. When opened in response to the voltage difference across the membrane, KCNA10 channel selectively allows the flow of potassium ions across the membrane down their electrochemical gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.8/KCNA10 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DPA9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024779"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009299.2">
        <property type="protein sequence ID" value="ENSPNAP00000024779.2"/>
        <property type="gene ID" value="ENSPNAG00000009480.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159534"/>
    <dbReference type="OMA" id="TTFCVET"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000033">
        <property type="entry name" value="potassium voltage-gated channel subfamily A member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000012">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF286">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="18">
        <location>
            <begin position="120"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="528"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="540"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024779.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="559" mass="62829" checksum="FD317FCC77AF802C" modified="2024-11-27" version="2">MEVPLVNFENLDDIGINLGDPSDSGYPTSPTSEAPEANQMTPGMNSPTHSPQRHRQHGNTPASPTTLTKKGTSSCNSLVSNWKILMNSEGSPGDALLGKLAKDCCEDLFGEKQKLEEGDQRVVINVSGMMYETTLKTLNQFPDTLLGDPKRRIDYFDPMKNEYFFDRNRPSFDGILYFYQSGGKIRRPANVPLDVFADEIVFYRLGHEVMEQFREDEGFIKEPEPQLPTSELHRQFWLLFEYPESSSAARSVALVSVLVITISICIFCLETLPEFRDDREFTHETVNLTRDANGTLLSPSPLPKIRESVFTDPFFVVESICIIWFCFEAGVRFVVCPSKSEFFSNIMNMIDIVSIMPYFITVITELMATHGDDAAANQNMSFATLRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAIYFAEVDEPETQFVSIPEGFWWAVVTMTTVGYGDMCPVTLGGKMVGILCAIAGVLTIALPVPVIVSNFNYFYHRETEQAEKHVMDAAAEAAANQKNSDEKYESTYSLDKSNGNWQMEKNGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQ85</accession>
    <name>A0A3B4DQ85_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCAAT/enhancer-binding protein zeta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CCAAT-box-binding transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CEBPZ</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Stimulates transcription from the HSP70 promoter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQ85"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025643"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007091.2">
        <property type="protein sequence ID" value="ENSPNAP00000025643.1"/>
        <property type="gene ID" value="ENSPNAG00000010829.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006395"/>
    <dbReference type="OMA" id="EIWCNDE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000805">
        <property type="entry name" value="Similar to transcription factor CBF/MAK21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040155">
        <property type="entry name" value="CEBPZ/Mak21-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048">
        <property type="entry name" value="CCAAT-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12048:SF0">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="12">
        <location>
            <begin position="533"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="114"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="642"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="876"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1058"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="123"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="642"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="659"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="686"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="886"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1077" mass="122941" checksum="F8F69CD868DA21BA" modified="2018-12-05" version="1">MTGVFFGVSSMAAKGKQRPKAVRSEENEEDTFENDNDEAEEGKQDGDDEFTLEDVLRLGGTKADYVMLVGLDDNNELVNGGKKGAIDDLEDGELEQFITKLGICNYLDQQIVKEDGDDDEQEEKPTEPAEEKEVKKKEPSKAESVKAKQEAERKKKDKEAKKAAAALKKAKQNASIFEFYPRQTLLVKPGGKWYDIDYTSETTSSAQDESLVSQYKVLAQKLFEAEAELYKTKKNFQKGANTTWMKNVVSTGTLADRMAALTILIQDAPVHCLEHIESLVTLIRKKGSRREGLMAVETLKELLLSDLLPENRKLRAFAQRPFNELEERASGNRDVRDRRLVLWYFEHKMKLLMAEFVVALDTLAHDSILASKTKALTTAHELLCNRPEQEKALLAQIVNKLGDPEYKVASKASYLLEMLLHKHPNMKGVVCTEVENLMFRPNISPKAQYYATCFLNQVMLSHEEADLATKLITIYFTFFRVCVKKKDVESKMLSALLTGVNRAYPYTKMGDKKVQEQMDTLFKVVHMVRFSTALQALMLLFQVMNSQQSVSDRYYVALYRKLLDPGLSVCSRQSMFLNLLYKSLKTDVVLRRIKAFVKRLLQVSCEQSPAFACGALFLVSEVMKSKPGLKLLLQEQEDAEEEKFEDLKGEDDDEDEERFVDAEKVEEGQSQKPELPKPTASWVHHKNLEGGKSRESYDPTHRNPLYCGANYTTLWELQKLCLHFHPSVALFARTIMQGEFIHYTGDPLQDFTLIRFLDRFVFRNPKQMKGKQNTEATVMQPKHRQSMNNIRSLPVNSEEYLAKEESQIPVDEVFFYRFFKKREGEKRLQKPRGGDDESVEDVDDDEFDRMLDTFEGDSFFADFKDAELDFAGNVKDKAKKGKKDEDSDLDSEDDLDDEEVSLGSMDEEEFGDELEEEGGEFMDLEGEDEEEVPELEDDGDFGDGEDEDLDEPADVRTKLKKGKRKSSEQFGFSGSLGLKPEKKKKKGKKEDVAMFASAEEFGELLDENVGSMFDNLGMNAMANKDKASVKQLKWEAQRDDWIRGCDVKTLRRKKNMFKKRKQFGKAKPAKRTAGRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTU0</accession>
    <name>A0A3B4DTU0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CFAP20DC</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTU0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026508"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004946.2">
        <property type="protein sequence ID" value="ENSPNAP00000026508.1"/>
        <property type="gene ID" value="ENSPNAG00000011806.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005497"/>
    <dbReference type="OMA" id="KSHIAFG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040441">
        <property type="entry name" value="CFA20/CFAP20DC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12458">
        <property type="entry name" value="ORF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="774" mass="85420" checksum="F24C8E986D6FC804" modified="2018-12-05" version="1">MYICAFAMFKGGAIVEIFSAQGKDPVAKWKLSGGQASISKVFDKEVKGFVYSLEGSSQTHRMQLPKDGKTPLVLIQRFLVLQVNVPPGKDFSTELVVTDQGHLKRRLYLSTVHKEFSATPLHARIPLTGLRRNIWCNLCIDMGSFTAELFRGATFLSLEGIVISACCKVKRIFSMRAEPADHVDRDLYGIRNGPKDEIPKSCQFPPDVQHVTLLVNMERLGQVEVRNAPINSESEQTGTGRAATARGPKTQDSSHIAFGSKVAGRPPLTARKSSSSVNIAEPKARTDPSISALSGRSGNLQPRPPTERASSERMRSRRPQQIHSGGRKSSVTRNNGDIPYPSHEGEESSQRASQHLIQPDCSSIVQQDPPSEQYIPGQACISETPTPRPLQCPSPSALLPHLCVQENSINDISDTEPQLSEREAEEEDGEELFTFSSRPHSAKRGQPISDPFVDLDSEIKGDSLRGEPERREARLEDDFIGSEGEEDDRYSPFQRFAAHSSPASPTPLSVPVHKSTTSPRPCTDSAQSQPSTAPPASSHSATGSSWRTEPACMAPTRCLSPSTARQRSRLEWSGAERTKPDGETGTSISRTSVREILSGDLRLHKVRESLRSTPVGSSRCDLHKRPDQEVDEEEELRMLASLRREQEEEEGGMSDPGLSASQVDQCNVSLSMSSDDTSTWTQCIRLAAEQGQYYQKEMNPLLHSNPREWMDVLSPPIVHPSQQLTQSRGDHSKISTKGSEAQENSGEDEFLSLLYDPCLNCYFDPQTGKYYELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTX2</accession>
    <name>A0A3B4DTX2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017557209.1">
        <property type="nucleotide sequence ID" value="XM_017701720.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017557210.1">
        <property type="nucleotide sequence ID" value="XM_017701721.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017557211.1">
        <property type="nucleotide sequence ID" value="XM_017701722.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017557212.1">
        <property type="nucleotide sequence ID" value="XM_017701723.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017557213.1">
        <property type="nucleotide sequence ID" value="XM_017701724.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017557214.1">
        <property type="nucleotide sequence ID" value="XM_017701725.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTX2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027827"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001572.2">
        <property type="protein sequence ID" value="ENSPNAP00000027827.1"/>
        <property type="gene ID" value="ENSPNAG00000013348.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429740"/>
    <dbReference type="CTD" id="541526"/>
    <dbReference type="GeneTree" id="ENSGT00940000154890"/>
    <dbReference type="OMA" id="MACGPCM"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061762">
        <property type="term" value="P:CAMKK-AMPK signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000429">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000091">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase kinase 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF26">
        <property type="entry name" value="CALCIUM_CALMODULIN DEPENDENT PROTEIN KINASE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="114"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="479"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="508" mass="56674" checksum="844337DFD0A1859B" modified="2018-12-05" version="1">MAMSSDPGCMGTEPDPDPEPFSQSELADKMAAITITDPSTNGLRNGAGVRKVPPQRPHLSGRKMSLQERGTYLSAGGGAERYHHISPRAARRPTIESKRVSISDAPDCIQLNQYKLKSEIGKGSYGVVKLAYNEDDDKYYAMKVVSKKKLMKQYGFPRRPPPRGPKAALGEQPKVLGPLERVYQEIAILKKLDHLNIVKLVEVLDDPAEDNLHMVFELMQKGPVMEVPTENPFSEEQARLYFRDIVLGIEYLHYQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDALLSSTAGTPAFMAPETLSDNRKSFSGKALDVWAMGVTLYCFVFGKCPFIDEYILALHNKIRSKPVEFPETPAVSEGLRNLILRMLDKNPDNRITIPEIKLDPWVSQDGTDPLPLEEEHCTVVEVTEEEIQNSVKFVPSLSAVILVKAMLRKRSFGNPFECPSRREERSMSAPGSLLMDSWPFRPSLKLHPSLRKGSSGEGGREVELEDLHEDEPPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DWX6</accession>
    <name>A0A3B4DWX6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">SLIT-ROBO Rho GTPase activating protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017568254.1">
        <property type="nucleotide sequence ID" value="XM_017712765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DWX6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027489"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002428.2">
        <property type="protein sequence ID" value="ENSPNAP00000027489.1"/>
        <property type="gene ID" value="ENSPNAG00000012990.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436338"/>
    <dbReference type="CTD" id="561231"/>
    <dbReference type="GeneTree" id="ENSGT00950000182824"/>
    <dbReference type="OMA" id="ECAGARG"/>
    <dbReference type="OrthoDB" id="5981864at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07656">
        <property type="entry name" value="F-BAR_srGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04383">
        <property type="entry name" value="RhoGAP_srGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000026">
        <property type="entry name" value="Rho GTPase activating protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000136">
        <property type="entry name" value="Rho GTPase activating protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000059">
        <property type="entry name" value="Rho GTPase activating protein 4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051627">
        <property type="entry name" value="SLIT-ROBO_RhoGAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14166">
        <property type="entry name" value="SLIT-ROBO RHO GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="22"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="489"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="714"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="772"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="909"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="7"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="773"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="823"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="859"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="916"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="963" mass="107354" checksum="E01B4FC93664B1E3" modified="2018-12-05" version="1">MTSHGKLRKEKGSLAEYETQIKEVRSQLSEQLKVLDAQLEQKTQQLQDLSDYLRRRGEIEAEYARSLEKLSERFTLKTKKKEQSDQSVAQCWSVLLAQTKQESRDHSSLSEMCSTTLTQRLSHCIEDTHRLAKKSKEVGLQMQDELLKVTTELQTALKTYHQYHTECLAAEGKLKEATRLEERQTGKSADLGLGQSAGQRRSSVKKMERLMEKRQGKVQETQLKCTKARNDYLLNLSAANAAMNKYYLEDICTLIDCTDLGYHLSVSRVMRGYLSNKNRAQQNLKNCLQQLDDSISGLDQGQDRDALLQAHNSAFCLPFRFQHQPHEGDQVCEVSADCQVRYEMETRFQQLQSRLAAVTLETEEVSKTLKATHTALLDNICDDDCNPTPDISATLSLEGTGDGPGTKQSLAKRRANQQETETFYFTKVKEYLNGSSLISKLQAKHDLLKEAIQKAEAIDSDPSRMQPGRAVRMRKARPCSQYNHKLFTGDMVSFIQSSGQQIPLVVESCIRFINLHGLHHEGIFRVPGSQSEVNHIRDAFERGEDPLTDSECDIDSVAGVLKLYFRGLEKPLFPEESFNQLMECVQIENVAERAAQIKTVISSFPRPIIIVMRYLFAFLHHVSQYSDENMMQPYNLAVCFGPSLLRGAESGDAVTLQPQINALVKTLILQHESIFPGPAELPGPVYEKCMTLEQEYCEPITEEGEGDAEHVPSEDEWDAVAMFDYVARSAAELSFKQGDHILLHSKASSDWWRGEVGGVKGLIPHKYISVSEGAERKQERGKREDARGGSTGNLAEEQQLAEHSTRMRVNSDSASLPGRQRTGSGGGGMGGGGSGSGSPVRKFTLQVPDGRFILPPQSAGATRQVSGSQERRHTLDSLRPIGGPADRQTVHVDKEISRQMNSVFKELLSRQPAQDSSVSQSPSSPSSSSTSSVSTGVTRQSVKKGTPFSLRGRGLFKPPDQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXU3</accession>
    <name>A0A3B4DXU3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXU3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028293"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000340.2">
        <property type="protein sequence ID" value="ENSPNAP00000028293.1"/>
        <property type="gene ID" value="ENSPNAG00000002987.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286931"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017296092" description="RING-type domain-containing protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="93"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="223"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="261"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="283"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41867" checksum="47B12447FE1257A0" modified="2018-12-05" version="1">MDKNIWTWIRKGFICLLCNSLIVYTQNSKTCKTGPTTKPHLFTYLLCQPSFSLSHKHKEASFCSDRLISTTPDYSESVKMAEVAQEDQDPFSCPICLDPIKDPVTIPCGHNYCMACIRDYWNEREHTETYSCPECRENFRPRPVLNKNTMFAEVVERFKKAKLQDASPASCGPRKCSMCAGQKVQAVKFCPQCEESSCEAHTARHNSMSLREKHTVIVVGGEPPKNICVLHNMPLEIYCQTDQQLICSLCFVEHHRGHDAVSVGPKNTERQVDINSPQNNGGRRPRNRHRSHEHGRRRHKQSRHGRGHGSGHRKSLHSKCGHEAGHHESHGCTLGRHGHAEGGGHTLGRNRSGTHGGRHRSRRHERSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZJ5</accession>
    <name>A0A3B4DZJ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase</fullName>
            <ecNumber evidence="1">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CHST15</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZJ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028424"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014622.2">
        <property type="protein sequence ID" value="ENSPNAP00000028424.1"/>
        <property type="gene ID" value="ENSPNAG00000014113.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004719"/>
    <dbReference type="OMA" id="MWDKKGL"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="260"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="571" mass="65705" checksum="CD961BEAB64F9885" modified="2018-12-05" version="1">MKVQTHSAPCSVRPCPRPTPCLSGSEGSLPGARGWPGGAMAHLHYKYSLLSSAAEEYHKRPLPLQLDYAPLGVFAVLEVQRELQRPWTPMSCLRRPKVYSFIFGLTIIFFIMASYVLSSERKGLLLTPSPYHADSSLNLGTLPFNFSSNKDHGHITQVLHQILSKVEFSPRKLPDLRMLVHSEPHMFSVIPRHFLPDVKNPCWYEQLSGNVSADPYRRNLYSLYSRQSRATFQYLRSSFRKHLLFRDGKFFRIRCLPYFYIIGQPKCGTTDLYDRLRLHPDVRVTALKEPHWWTRKRFGIIRPSDGLHARYPVEDYLDLFDLPAHQIQDRLLANSSGSSNQANIIIGEASASTMWDNNAWAYFCDNATEGEPPFLIQDFIHALQPEARFIAILRDPVERLYSDYLYFGMANKSSEDFHEKVWESVQLFDACLQDFSMRACVYNSSLNNAMSVRLHVGLYVVYVLDWLSVFGRDQLLVLRLEDHAADRTLSMHRVFQFLSLRSLSEQKEAEITKRPASNTRRLSDKNLGPMLPLTREILSDFYGPFNRKLAAVLQDDSFLWDTHSKPASRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E443</accession>
    <name>A0A3B4E443_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Active breakpoint cluster region-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ABR</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017554524.1">
        <property type="nucleotide sequence ID" value="XM_017699035.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E443"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030458"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018520.2">
        <property type="protein sequence ID" value="ENSPNAP00000030458.1"/>
        <property type="gene ID" value="ENSPNAG00000017401.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428209"/>
    <dbReference type="CTD" id="29"/>
    <dbReference type="GeneTree" id="ENSGT00940000153491"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08686">
        <property type="entry name" value="C2_ABR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04387">
        <property type="entry name" value="RhoGAP_Bcr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000057">
        <property type="entry name" value="active breakpoint cluster region-related protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000014">
        <property type="entry name" value="active breakpoint cluster region-related protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000004">
        <property type="entry name" value="active breakpoint cluster region-related protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.30">
        <property type="entry name" value="Bcr-Abl oncoprotein oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037769">
        <property type="entry name" value="Abr/Bcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015123">
        <property type="entry name" value="Bcr-Abl_oncoprot_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036481">
        <property type="entry name" value="Bcr-Abl_oncoprot_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182:SF5">
        <property type="entry name" value="ACTIVE BREAKPOINT CLUSTER REGION-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182">
        <property type="entry name" value="BREAKPOINT CLUSTER REGION PROTEIN BCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09036">
        <property type="entry name" value="Bcr-Abl_Oligo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19057">
        <property type="entry name" value="PH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69036">
        <property type="entry name" value="Bcr-Abl oncoprotein oligomerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="DH" evidence="12">
        <location>
            <begin position="337"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="547"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="731"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="13">
        <location>
            <begin position="894"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="146"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="219"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1107" mass="125487" checksum="A621DEA6D9151EA9" modified="2018-12-05" version="1">MWEQEEFEKHWRNEFPDGEVPKMNLSSVEDVEKELERCKAKLKTLQQALSEEKFKVIYLQTTIAQKKKSYDTERWGSKEEDEAPTPAAAVVSEVDGNKPAGVAGKVERLDSKVHTRVKLPGLGSIGSKGSEELSSSLAARLDVARGSKTGKPILVPPRKKPVLLKESSSAPVLEQQPVRDLVPQQQKVEERDSDHEYEDVELNENFVRRNLLNPKAKAQLRWSRDLDSGDEGRLSPSVPSRCSFGSGRSTPDRRSDGYISSDHDDSSSADFNYNTDGYEGDGAEDRKSQDGSETMPFIDESPTMSPQLCVRPDGEAVSPTPPEGLLPGGDIEKGLEMKKLVLSGFMASEEIYINQLEALLLPMRPLKATATTSKPVLTMEQIETIFYKIQDIYEIHKEFYDALFPNIQQWDDKVTVGHLFQKLASQLGVYKAFVDNYKVALETVEKCSQANSQFQKISENVKVKGPKDSKDCTTTVTMEALLYKPIDRVTRSTLVLHDLLKHTPKEHPDFALLQDALRISQNFLSSINEEIDPRRTAVTTPKGEARQLVKDGFLVELSEGSRKLRHVFLFTDLLLCAKMKKTAVGRNQQYECKWYIPLADLTFQTLDDSDSCPHVQTLPEHEIEEMKIKISVIKSEIQKEKKAQKGQSRIVDRLRKKMNEQESCLLLHSPTIPFRIHSKHGKSYLFLLSSDYERSEWRESIQKLQKKDLQSCVLSTLELQVLTSSCFKLRTVHNIPVTSNKDDEESPGLYGFLHVIVHSATGFKESANLYCTLEVDSFGYFVSKAKTRVFRDTTEPHWNEEFEIELEGSQCLRILCYEKCYDKSKLNKDDNEIVDKIMGKGQVQLDPQTVQSKNWHVDVIEMNGIKVEFSMKFTSRDLSLKRTPSKKQSGVFGVKISVVTKRERSKVPYIVRQCIEEVEKRGIEEVGIYRISGVATDIQALKAAFDTNTKDILVMLSDMDINAIAGTLKLYFRELPEPLLTDRLYPAFMEGIALSDPAAKENCMMHLLRSLPDPNLITFLTLLEHLKRVAEKEPVNKMSLHNLATVFGPTLLRPSESESSKPHITLASDIWSHDVMAQVQVLLYYLQHPPISFAELKRNTLYFSTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4Y0</accession>
    <name>A0A3B4E4Y0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EBNA1 binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EBNA1BP2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the processing of the 27S pre-rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EBP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558886.1">
        <property type="nucleotide sequence ID" value="XM_017703397.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4Y0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030753"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019084.2">
        <property type="protein sequence ID" value="ENSPNAP00000030753.1"/>
        <property type="gene ID" value="ENSPNAG00000017674.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430739"/>
    <dbReference type="CTD" id="10969"/>
    <dbReference type="GeneTree" id="ENSGT00390000014984"/>
    <dbReference type="OMA" id="RETMFHR"/>
    <dbReference type="OrthoDB" id="443772at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="217"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="276"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="35145" checksum="75B25938E5DE316B" modified="2018-12-05" version="1">MADVEMETQMGLDSEEDYGELSDAELQEAFSKGLLKPGLNVPLEEPKKAVNNIEGLKMCLAEFKKNLPWVERLDLTNHPAVDVLAKIEGTLNKKQSSGEIDAEDDFQREMYFYRQAQATVLEALPKLKKLQIPTKRPDDYFAEMAKTDQHMQKVRKKLMQKQMAIEKSEKAKKLREQRKFGKKVQVEVLQKRQKAKKAMMTAVKKYQKGMTDKLDFLEGDQEGKKGSTASKSQMSKKGPNAKRKYKDQKFGFGGKKKGSKWNTKESFNDVSGFRAKTAHGKGGKKFNKGGKKNKRPGKETRRKMKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E699</accession>
    <name>A0A3B4E699_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetylserotonin O-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Hydroxyindole O-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ASMT</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.4" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017573468.1">
        <property type="nucleotide sequence ID" value="XM_017717979.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E699"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032147"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021810.2">
        <property type="protein sequence ID" value="ENSPNAP00000032147.1"/>
        <property type="gene ID" value="ENSPNAG00000019935.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439529"/>
    <dbReference type="CTD" id="438"/>
    <dbReference type="GeneTree" id="ENSGT00940000161561"/>
    <dbReference type="OMA" id="FWPYVFG"/>
    <dbReference type="OrthoDB" id="1606438at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017096">
        <property type="term" value="F:acetylserotonin O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030187">
        <property type="term" value="P:melatonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000358">
        <property type="entry name" value="Acetylserotonin O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000146">
        <property type="entry name" value="Acetylserotonin O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0471">Melatonin biosynthesis</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="13">
        <location>
            <begin position="14"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="12">
        <location>
            <begin position="119"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="345" mass="38795" checksum="AB2B8BA1DC86EB9E" modified="2018-12-05" version="1">MAVVKEDVYPKKILEYMEGYLVSKTLFAACELGVFDLLQDSQRPLATAEVAQALDSSEDGMDRLLSACAGLELLTTQPTTDGQVLYSNTEMSSMFLTKSSPRSLYHSIDYNSNTMYLCWHYLTNAVREGKNQYERAFGVSSKDLFEAMYRSDEEMVKFMQLMNSIWNICGRDVVMAFDLSPFKSVYDLGGCSGALARQCVQIYPDCNVTIFDLPKVVHMSREHFPSDDRIHFHEGDFFKDSLPEADLYILARILHDWTDERNVELLTKIHQSCSPGGGVLLVEALLSEDNTGPLTAQLYSLNMLVQTEGRERKGSEYAALLTKAGFTTVHIHKTGKVYDAVLALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EEB4</accession>
    <name>A0A3B4EEB4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">General transcription factor IIF subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Transcription initiation factor IIF subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570598.1">
        <property type="nucleotide sequence ID" value="XM_017715109.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EEB4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034867"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027118.2">
        <property type="protein sequence ID" value="ENSPNAP00000034867.1"/>
        <property type="gene ID" value="ENSPNAG00000024442.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437770"/>
    <dbReference type="CTD" id="415217"/>
    <dbReference type="GeneTree" id="ENSGT00390000016051"/>
    <dbReference type="OrthoDB" id="26094at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07980">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015849">
        <property type="entry name" value="TFIIF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="12">
        <location>
            <begin position="16"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="11">
        <location>
            <begin position="192"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="265" mass="29826" checksum="5328C91B934FE0B3" modified="2018-12-05" version="1">MSDRGEVDLTGAKQNTGVWLVKVPKYLSQQWAKATGRGEVGKLRIGKNQGKAEVSFTLNEELTTIDSSSGKAPVVRAPREHPFTMQTVGGQTLAVFTETSSGQSDAEGSSSGTGTGPDKIALEGVVVQRAECRPAASENYMRLKRLQIEESSKPHRFSQQLDKAVTTNYKPVANHAYNLDYEKKKKEEGKRARADKQQVLDMLFSAFEKHQYYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHYQNEEKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EEP6</accession>
    <name>A0A3B4EEP6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Cappuccino subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EEP6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034310"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026066.2">
        <property type="protein sequence ID" value="ENSPNAP00000034310.2"/>
        <property type="gene ID" value="ENSPNAG00000023517.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154289"/>
    <dbReference type="OMA" id="PERTHCI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001265">
        <property type="entry name" value="Formin_Cappuccino_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF7">
        <property type="entry name" value="FORMIN-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00828">
        <property type="entry name" value="FORMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="260"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034310.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="636" mass="71242" checksum="BCD62659907049E0" modified="2024-11-27" version="2">MNDYYLPQSYESEERTPGRLQAVWPPPKPKDKEEKIGLKYTEAGKSACTKKQMQMFALSHIFALHVMTAIAELKEKAKRSHGDLCDVAVSTEDNVTPRAFRTVCIQTDRETFIKPVEDAELSSGLCPQPVVPKKLDLTSINLSLSGKQPEAKQGLPPLALPLPPPPPPLPVQSESETNRAPPPPPSLSGHCDSNDFSKPDKSLDRHLPLEKGSGPPPPLPPPPMAGYGLPPPPPPIPGSAPSLPTGTSLLLSKGEEWPQRKPRVEPVCPMKPLYWTRIHVQNNRNDTLWSSLKEPAIINTNEFAELFAKMSSPAKRKPLSEAYEKKGKAKKIIKLLDGKRSQAVGILISSLHLDMKDIQQAVLMLDNSVVDLDAIEALYENRAQPEELERIRKHYETSDEEQVKLLDKPEQFLYELSRIPEFSLRACCIILQSTFADAIASIRRKTEIVLHVCKDLLEQDSVKEVMGLVLALGNYMNGGSRTRGQADGFGLDILPKLKDVKSRDNHTSLLDYVVSYYLRNLDENAGTENCIFPLPEPQDVFLAAQVKFEDLSKELRKLGKDLADEPCSLWTVVLPFSPSLLTPLLSFRDLVEYFGLKPRSGEQDVLPGHVFMLWFEFCTDFKTRWKRENKAISKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGC8</accession>
    <name>A0A3B4EGC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RAB5 interacting factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553678.1">
        <property type="nucleotide sequence ID" value="XM_017698189.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGC8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034349"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026084.2">
        <property type="protein sequence ID" value="ENSPNAP00000034349.1"/>
        <property type="gene ID" value="ENSPNAG00000023602.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427742"/>
    <dbReference type="CTD" id="55969"/>
    <dbReference type="GeneTree" id="ENSGT00390000004786"/>
    <dbReference type="OMA" id="ANSEWPD"/>
    <dbReference type="OrthoDB" id="286395at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010742">
        <property type="entry name" value="RCAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12906:SF0">
        <property type="entry name" value="GEL COMPLEX SUBUNIT OPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12906">
        <property type="entry name" value="PROTEIN C20ORF24 RAB5-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14438" checksum="801628F775C13D70" modified="2018-12-05" version="1">MKNSTKRKEEAAQSNGVKQSTWSKALRSGAVWEDKDEFLDVVYWFRQIIAVVLGIIWGVAPLKGFLGIAIFCIINAGVLYVYFSSFQQVDEEEYGGTWELTKEGFMTSFALFLVVWIIFYTALHYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EI63</accession>
    <name>A0A3B4EI63_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GCF C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PAXBP1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GCF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537355.1">
        <property type="nucleotide sequence ID" value="XM_017681866.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EI63"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028829.2">
        <property type="protein sequence ID" value="ENSPNAP00000035555.2"/>
        <property type="gene ID" value="ENSPNAG00000025922.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410663"/>
    <dbReference type="CTD" id="94104"/>
    <dbReference type="GeneTree" id="ENSGT00390000000455"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012890">
        <property type="entry name" value="GCFC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022783">
        <property type="entry name" value="GCFC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214">
        <property type="entry name" value="GC-RICH SEQUENCE DNA-BINDING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214:SF2">
        <property type="entry name" value="PAX3- AND PAX7-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07842">
        <property type="entry name" value="GCFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GCF C-terminal" evidence="5">
        <location>
            <begin position="579"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="240"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="510"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035555.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="902" mass="102012" checksum="51FE7EB6A95A091F" modified="2024-11-27" version="2">MFKKARRCNFRRRNESEDEEKDELLSAEQPPPPPPASYGPAAAAAGDLGAASGESNAVNSHGNGFQAAAVKTAKEKKKKKESREEPKASLLSFNDDEEDTEVFRVKKSNHSKKIVKQLKKEYREEKSVCVAHDAAPRLDGVPSLPSTGVKDEPEDSRGSSEQGEGEMEVDSNDEQDDESEAKSSGGGAFSQTLSALSSLRPGEIPDAAFIHAARKRRQMARELGGETPLVDMEVANKRLVREDEQGGSDEDEKRIVFSGVTQKSQRQKIAEEIGIEGSDDEALDTAGNDEEVSRWEQEQIRKGISIPQVQTSQPEDTNMYYQGGYEGQPYGVSYGMPYGYAAVGADSSKPVKAESSVPYPIPSSSLTPITTALVKKRLTDRLSSMRQGRDASAQRYEQIQEELQASNSSILQLEESSQHTAEQYKFLQEMRGYVRDLLECFSEKVPAVLELEAAMHQLLRQRAVRRVQRRQDDIKDESAEFSSLSSKAVMAPNLDPFGRDRAAYQELSKQRRIAEREARRARRRQAREQSGKRAEHKEGLSSDDEETSTDITSFNLEKDRILKESKKVFEDVIEDFHSLDCIKSRFEVWRKLYFTCYRDAYIGLCLPKLFNPLIRLQLIPWSPLEDDCPNFEYMLWFESLLFYGCEEQTNTQEEDIDTGLLPAIIERVILPKLAVLSDQVWDPLSRSETCRLVAFMRRLIKGYPTVLHGENRNTQELLRTIVIRTRRSLDEDVFMPLFPKNVMENKNSGPYLFSQRQFWSCVKLLGNVLQWDGILSQNTLKELAIDSTLNRYILSALQMADVGEDSVEKCQQVVECFPVHWFSTLKGQQTLPELENLCRYMKHLASSLYRSSLTASDVDKRSAREHIKEVVKVLGRLNALDHVIAVASEHGIKDIKTLLESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJC8</accession>
    <name>A0A3B4EJC8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017542021.1">
        <property type="nucleotide sequence ID" value="XM_017686532.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJC8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035985"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029768.2">
        <property type="protein sequence ID" value="ENSPNAP00000035985.1"/>
        <property type="gene ID" value="ENSPNAG00000026406.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413846"/>
    <dbReference type="CTD" id="56731"/>
    <dbReference type="GeneTree" id="ENSGT00940000156172"/>
    <dbReference type="OMA" id="IFKGAPG"/>
    <dbReference type="OrthoDB" id="5950721at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052253">
        <property type="entry name" value="CR1/CR2-DNA-binding_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006">
        <property type="entry name" value="PAPILLOMAVIRUS REGULATORY FACTOR PRF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13006:SF8">
        <property type="entry name" value="SLC2A4 REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01366">
        <property type="entry name" value="c-clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="224"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="513" mass="55578" checksum="94253087BB1F9226" modified="2018-12-05" version="1">MTSKRLLDKSPAGVRASCAGSIITHAGHGEGVAAVDYGVLWARKKGAIAVNGFNTKLLDKGSLHEPSKETSLNGNHCSGREKHYSRRKMGCAGEEDGREVASSGGVLKRGSPTHLLVLNGQRPEEHIHVEAMTPVRRPQEPRCVLPLRVTDPSPEDPDASSHIPVPRNRNPSGHADKEVMAATVLTTLSTSPLVLNPSSGASGAVPEPASRGWKESLSASYSSSTSGNWSWDASDQSVPSTPSPPLSNDVSKSFLLSSQGEDNIEEAESTHFLFEDPIPRKRKNSVKVMFKCLWKNCEKVLSTSSGIQRHIRTVHLGRNGDSDYSDGEEDFYYSEIEVNMDTLSEGLSNLTPTSPTTAGPPPVFPLASDMAQSESYMIHSPLSQSAPSTLCHIRTDHAYQATAPVNIPSVSSELPHTQSTISVSWQSPPVIFKGPPGALNHVRTVSIGEKRQPVTHTTVSKTHTINLSTPKPTAGTRKPRGEAKKCRKVYGMEKKDLWCTACRWKKACQRFTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJT1</accession>
    <name>A0A3B4EJT1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Barrier-to-autointegration factor-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Barrier-to-autointegration factor 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017560447.1">
        <property type="nucleotide sequence ID" value="XM_017704958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJT1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036028"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029884.2">
        <property type="protein sequence ID" value="ENSPNAP00000036028.1"/>
        <property type="gene ID" value="ENSPNAG00000026487.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431661"/>
    <dbReference type="GeneTree" id="ENSGT00390000018613"/>
    <dbReference type="OMA" id="TEWCDAF"/>
    <dbReference type="OrthoDB" id="9997163at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.40:FF:000002">
        <property type="entry name" value="Barrier to autointegration factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.40">
        <property type="entry name" value="Barrier-to-autointegration factor, BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051387">
        <property type="entry name" value="BAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004122">
        <property type="entry name" value="BAF_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036617">
        <property type="entry name" value="BAF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47507">
        <property type="entry name" value="BARRIER TO AUTOINTEGRATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47507:SF2">
        <property type="entry name" value="BARRIER-TO-AUTOINTEGRATION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02961">
        <property type="entry name" value="SAM_BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01023">
        <property type="entry name" value="BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47798">
        <property type="entry name" value="Barrier-to-autointegration factor, BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00079764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="128" mass="14412" checksum="26B8A8D77CAD52C5" modified="2018-12-05" version="1">MQRCPLQQGLLSRRSQREQAGVFQTSSFKRVLITRQPEMSTTSQKHRDFVAEPMGDKPVTALSGIGEVLGKKLEEQGFDKAFVVLGQFLLLRKDRELFAEWLKDTSGANARQAASCSQCLTEWCDAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELF3</accession>
    <name>A0A3B4ELF3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMEM107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELF3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033275.2">
        <property type="protein sequence ID" value="ENSPNAP00000037302.2"/>
        <property type="gene ID" value="ENSPNAG00000028932.2"/>
    </dbReference>
    <dbReference type="OMA" id="YHFFNET"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF5">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037302.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="149" mass="17521" checksum="33E59B7E13AD9EAE" modified="2025-10-08" version="3">MYERDVLHISVLLFGMQNLRSPPIQFYFVREHKTQLDAKTHCRDKYTDLVTIHNVTEMRAVMAAVDGSDSDFVWIGLERETSPNFIWSNTKFNDLSCSTALPFIFSYKQTFCFINSAVFFYWPCVKAVFVFIYLYDFKKTSYPESVQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELI1</accession>
    <name>A0A3B4ELI1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tubulin-specific chaperone C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Tubulin-folding cofactor C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TBCC</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Tubulin-folding protein; involved in the final step of the tubulin folding pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551384.1">
        <property type="nucleotide sequence ID" value="XM_017695895.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELI1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036041"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029905.2">
        <property type="protein sequence ID" value="ENSPNAP00000036041.2"/>
        <property type="gene ID" value="ENSPNAG00000026533.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426440"/>
    <dbReference type="CTD" id="6903"/>
    <dbReference type="GeneTree" id="ENSGT00940000162058"/>
    <dbReference type="OMA" id="YFQHEIT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1250:FF:000001">
        <property type="entry name" value="Tubulin-specific chaperone C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000007">
        <property type="entry name" value="tubulin-specific chaperone C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1250">
        <property type="entry name" value="Tubulin Binding Cofactor C, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027684">
        <property type="entry name" value="TBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031925">
        <property type="entry name" value="TBCC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038397">
        <property type="entry name" value="TBCC_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139">
        <property type="entry name" value="TUBULIN FOLDING COFACTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16752">
        <property type="entry name" value="TBCC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="12">
        <location>
            <begin position="158"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036041.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="340" mass="37456" checksum="3845D600357AA08B" modified="2024-11-27" version="2">MEVFSAEVGPSEPVRIPESVLRRDQARQEELERRRNAKDSQSVSEENAAYFSAAFSAERAAIEELVSSCCSADHEKITNTLEEATLKSQQLQKFLNDSTMFLTQYELRQAQATLQKLQASIAELKPKKKFAFRSRTVTAGSSQQPPAAAASSGSTDKPAGLGTAVDGGAVSATQCGFSNVQNVVLIKRSEEIQQRDVLLSHLTNCKVWLYGSPSTLHIKNVRDCQILSGPVSSSVFVDECTGSTLGFACQQLRTHNTTHTQIYLHVTSRAIIEDCHGLLFAPFNWTYDGMDKDFKVSGLDPERNNWSEVDDFNWLAAGTPSPNWAIIAEPERISSWETAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ELT6</accession>
    <name>A0A3B4ELT6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Piezo TM25-28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ELT6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036743"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040254.2">
        <property type="protein sequence ID" value="ENSPNAP00000036743.2"/>
        <property type="gene ID" value="ENSPNAG00000027847.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164142"/>
    <dbReference type="OMA" id="DNFLHCS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031805">
        <property type="entry name" value="Piezo_TM25-28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF7">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL COMPONENT 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15917">
        <property type="entry name" value="Piezo_TM25-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo TM25-28" evidence="2">
        <location>
            <begin position="276"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036743.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="440" mass="50639" checksum="EFF2EC7289FD79D7" modified="2024-11-27" version="2">MYQLKFIKPSEYSLNCTTDLQVSNGSWYSGNVAHLLQKSVLYVEPVDPALWIGGLRKCENSILPCLWNHLLILGLLVFDVTIHRHQLYHRLHNDLKTPSKGAIFQNVTWQHLDHGVLPCLKYFCNYFFYKFGLEMSFVVAVNVIGQRMDFYAILHSFALIVVLGRRRRKAIGEMWPKYCCFTASLMVFQYLLCIGLPPALCTDYPWRKSTPAFTSNLIKWLYLPDFAMQPNPTFLLYDFLLLMAASLQWQVFEDENKASVRLLAGENVEISRSLNPRSLNQYTSVGNFLHCRSYLDMAKVFVFSYFFWLVLCLVFITGTTRINIFCLGYLVACFYFMLFGDTVLLQPVRYILKLWDCLIAYTSVVICLKNLISLGSCAYLDSLLKNGCWLIQAFSMFCTIKGYDVLASRRGWYCVGCDLFCCSVGSKENIPQLLLSVCCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EP34</accession>
    <name>A0A3B4EP34_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EIF5</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC. When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP. Start codon recognition also induces a conformational change of the PIC to a closed state. This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC. Finally, EIF5 stabilizes the PIC in its closed conformation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the 43S pre-initiation complex (43S PIC), which is composed of the 40S ribosomal subunit, EIF1, eIF1A (EIF1AX), eIF3 complex, EIF5 and eIF2-GTP-initiator tRNA complex (eIF2 ternary complex). Interacts with eIF1A (EIF1AX) during scanning. Interacts through its C-terminal domain (CTD) with EIF1 or with eIF2-beta (EIF2S2) (mutually exclusive) through a common binding site. Interacts through its C-terminal domain (CTD) with the CTD of EIF5B. Interacts with FMR1 isoform 6; this interaction occurs in a RNA-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EP34"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037705"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034481.2">
        <property type="protein sequence ID" value="ENSPNAP00000037705.1"/>
        <property type="gene ID" value="ENSPNAG00000029712.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016478"/>
    <dbReference type="OMA" id="YRYKMEK"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000018">
        <property type="entry name" value="eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
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    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
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            <begin position="418"/>
            <end position="428"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00018059"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00053999"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00065967"/>
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            <dbReference type="PROSITE" id="PS51363"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037705.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
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    </evidence>
    <sequence length="428" mass="48964" checksum="1B815B0C0B23D51B" modified="2018-12-05" version="1">MSVNVNRNVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKFFGCELGAQTQFDSKNDRYIVNGSHEANKLQDMLDGFIRKFVLCPECDNPETDLHVNPKKQTIGNSCKACGYRGMLDTRHKLCTFILKNPPESDSGSVKKEKEKKNRKKDKENGSGSGDAGNHNEIDAPDAVHGDDDDEDWAEETTEEAQRRRMEEISDHAKNLTLSEDLEKTLEERVNMFYNFVKQKKESGAIDSADKEILAEAERLDVRAMGPLILSELLFNENIRDQIKKYKRHFLRFCHNDKKAQKYLLGGFECVVKLHQTQLLPRVPIILKDLYDADLLEEDVILTWAEKVSKKYVSKELAKEIHAKAAPFVKWLKEAEEESEGSEEEEKEEDENVEVVYSSSARELKVETVQPEKKEEDDDLDIDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ERL9</accession>
    <name>A0A3B4ERL9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytoplasmic protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017705067.1"/>
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    <dbReference type="STRING" id="42514.ENSPNAP00000037939"/>
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        <property type="protein sequence ID" value="ENSPNAP00000037939.1"/>
        <property type="gene ID" value="ENSPNAG00000004692.2"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:signaling adaptor activity"/>
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        <property type="term" value="P:cell migration"/>
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        <property type="term" value="P:ephrin receptor signaling pathway"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of actin filament polymerization"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of translation"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="NCK"/>
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        <property type="entry name" value="SH2"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051184">
        <property type="entry name" value="Tyrosine-phos_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969:SF16">
        <property type="entry name" value="CYTOPLASMIC PROTEIN NCK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969">
        <property type="entry name" value="SH2-SH3 ADAPTOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF037874">
        <property type="entry name" value="Cytoplasmic_NCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
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    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6 10" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="107"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="190"/>
            <end position="252"/>
        </location>
    </feature>
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            <begin position="283"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR037874"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037939.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="380" mass="42977" checksum="DED87D3580430C31" modified="2018-12-05" version="1">MTEEVIVIAKFDYMAQQDQELDIKKNERLWLLDDSKSWWRVRNATNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKTGMRETASSAEAEFYADNGERLYDLNLPALVKFNYTAEREDELSLVKGTRVIVMEKCSDGWWRGSYSGHSGWFPSNYVTEDADGSASGDMGLSEKLAAVVHSANGNRVLHTVQALYPFSSVNDEELNFEKGEVMDVLEKPENDPEWWKCRKADGQVGLVPKNYVTVLQESQNSANMAGPPTPDCDYIEPSMSGRFAGKEWYYGKVTRHQAEVALNQRGTEGDFLIRDSESSPNDFSISLKAQTKNKHFKVQLKDNLYCIGQRKFGSMEELVEHYKKAPIFTSEQGDKLYLIKALPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ERN1</accession>
    <name>A0A3B4ERN1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017549442.1">
        <property type="nucleotide sequence ID" value="XM_017693953.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ERN1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037949"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034889.2">
        <property type="protein sequence ID" value="ENSPNAP00000037949.2"/>
        <property type="gene ID" value="ENSPNAG00000030053.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425361"/>
    <dbReference type="GeneTree" id="ENSGT01150000286944"/>
    <dbReference type="OMA" id="VEHECEV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11657">
        <property type="entry name" value="TIN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001009">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029400">
        <property type="entry name" value="TINF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF331">
        <property type="entry name" value="ZINC FINGER PROTEIN 322A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14973">
        <property type="entry name" value="TINF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="617"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="701"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="757"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="785"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="230"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="392"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="410"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037949.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="812" mass="91548" checksum="E10915D695F4FA67" modified="2024-11-27" version="2">MSSAPPISSLRLLVPPLRLMSAFMWQIAQRQNLEHYGKLEEFVSVVTKLVPEVLTSRQKATLIMGLRAKMILEMCRDEVPADFQTVKSHINSIQSSHSSKGSDPEVESLQANLLKLVLSLLEDPAKKEHFFQEVFPFEYGPDFDKALQVLVGHFLSRLEQLLPVPNFKQTSSLLGACHWELKECVESVCRTESLLPLMQSDICGPLEDNVLPSIVEERIISSLSLPPMMDTVLSSQSSDQPDTQPFLGAESPSHAPQVVEHECEVEDSCEHDEELTSGLDETASSEHPNTLQSPRANEGATEEEAERQNFEIEITEAIKHLEKESKMPTQVAESLPSTEMPEVSAEPKNTEGSESGVSEAPVAAVANTGQESKMQIPRIPLTKITLPLELSRSTRDAQNNDVPEEPGSFQEPSSPSAPLSSGKTSLQGSYLLSRRLYKCTECSKRFKYRSELLKHQQTHSSRTPPKCCECGQVFNEAKQLSLHRQKSCKMRTYKCIKCGAGFKSLSNWYTHQRTHKVVCTHKCPECGKVFRSLTGLVAHRREHTASTVNGGYTCDKCDLTFGSYRARVMHQKKHEIKPQVPPRKEREPGKCRFCDETFIVVSDLRVHLKTHPEYRPHQCDQCGKCFALRSSLLAHLSNHTGEKPLLCTQCGKRFFNKIQLKSHMRCHSGEKPHMCPHCGKCFSLLGNLNIHVRIHTGEKPYVCRQCGKGFISAGDLQVHQRSHTGEKPYCCTVCDKRFVVSSHLTAHKRVHTGERPFSCPQCGKTFVRRYDWSKHMYTHSGVKPYPCTICQKSYTRRTHLNRHMQSHVGHQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJ91</accession>
    <name>A0AAR2IJ91_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glycerol kinase</fullName>
            <ecNumber evidence="5">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJ91"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073786.1">
        <property type="protein sequence ID" value="ENSPNAP00000039648.1"/>
        <property type="gene ID" value="ENSPNAG00000003929.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214434"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07792">
        <property type="entry name" value="ASKHA_NBD_FGGY_GK1-3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000043">
        <property type="entry name" value="glycerol kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000033">
        <property type="entry name" value="glycerol kinase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042018">
        <property type="entry name" value="GK1-3_metazoan-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000756">
        <property type="entry name" value="PRK00047.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="17">
        <location>
            <begin position="13"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="18">
        <location>
            <begin position="277"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="555" mass="60872" checksum="FAAAC9C32F94B312" modified="2024-11-27" version="1">MSTSSRPEADSLVGAIDQGTSSTRFLVFNAKTAELISQHQVEINQSFPKEGWVEEDPKEILQSVCECMERTSEKLIQLNINISNVKAIGVTNQRETTVVWDKETGEPLYNAIVWLDLRTQSTVEKLIKKTPGKNKNHLKDKTGLPISTYFSALKLRWLMDNIESVAEAVQTNRAVFGTVDSWLIWCLTGGKNGGVHCTDVTNASRTMLFNIHTMQWDPELCKYFDVPIEILPEVRSSSEIYGYMNSGSLVGVPISGCLGDQSAALVGQMCFKDGQAKNTYGTGCFMLKNTGVKPVMSDHGLLTTVAYKLGRDQPVYYALEGSVAIAGAVVRWLKENLGIIKSSSDIEKLAATAGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNSSHLAFAALEAVCFQTREVLDAMNQDSGVPLSQLQVDGGMTANKLLMQLQADILCIPVVRPSMSETTALGAAMAAGAAEGVCVWTINPDGMTSVTSEKYQPKINPDESEFRYTRWKKAVQKAMNWETMEPVPTENGRHIMLTVGIIGSSDVQCSAKVWNQQKFGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKX3</accession>
    <name>A0AAR2IKX3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TNS1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKX3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087504.1">
        <property type="protein sequence ID" value="ENSPNAP00000038361.1"/>
        <property type="gene ID" value="ENSPNAG00000035808.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183062"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051971">
        <property type="entry name" value="E3_ubiquitin-PDZ_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545">
        <property type="entry name" value="PDZ DOMAIN CONTAINING RING FINGER PROTEIN 3, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545:SF4">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="876" mass="97545" checksum="5CFEED3AF49AAA09" modified="2024-11-27" version="1">MGCRLSGPWLGDGMGVNTRDLSHIRKREALRILASYEQPITLQIEGRRGQNLQFHRTSDCSTQTERPWDTLHTLAITHGPYSDRPYYSHVTLPGDHGDGGRYDYLPNAPRDLEDMRAIPCHDPELSARGPPGQSCLIGCCNTNLEEPSSFQSQTEDEDYMLEKTMGYLPLHHELDSGLGWTDGSFHQGELSGVETEEGLEECHTHRGGGGGGGSPSSESFISSELSDSGFYSVSTGEFLRFQRLLEKRMRLYNARIHHQGELRDSQKGHRELEAIPEALTVQPQNVCVPGGVGGHPRGLFRVSSVQFRKTERPCLNRHSSSSGALFTPHATLSTCSTPSSQRRMPPHHSSSTGTLRRSRTLHHRPPHVEHRRRASHPASPNYCMTLQNYGHRIQQELPEESETDVPHHANRPAHHANQAINYTNHPAHHASQATHHANQANQVPHHVNHPAHPADYATHHANHPTHPADYATHHANHPTHPADYATHHANHPTHPADYATHHANHPTHPADYATHHANHPTHPADYATHHVNHPTHPADYATHHVNHPAHSADYATHHVNQATHRAKHPVHSAEYATHHANHPVHPANYATHRANHPTHHANHAPHPATHHPHHTLPAHATLPAQHSHRLRPKHAISQPSLTSSFEDPHCDLPHLPHEPRRHDNGDRSPSSLREDRSFASEPPVGREKERSREREREARFHTLSHTPSQEVNDTWPKPASRQSTGGLYSTLEGHVTATSPTSAQKAPAGATNPRAVRNQLLRDRALRLADERGGMSTDEESHSEIRTGRYWSRTERREHMMQKKARSSSGAGTAITAAGGASAVLELSQRKLSRLRNRKLLDDWTTVEELLTHGSRLGVSDDALQIPSSLLTVTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IRS7</accession>
    <name>A0AAR2IRS7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein argonaute-2</fullName>
            <shortName evidence="12">Argonaute2</shortName>
            <ecNumber evidence="12">3.1.26.n2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Eukaryotic translation initiation factor 2C 2</fullName>
            <shortName evidence="12">eIF-2C 2</shortName>
            <shortName evidence="12">eIF2C 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Protein slicer</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 17" type="primary">AGO2</name>
        <name evidence="12" type="synonym">EIF2C2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include ago2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by ago2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.n2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of dicer1, ago2 and tarbp2. Note that the trimeric RLC/miRLC is also referred to as RISC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
            <location evidence="1 12">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However, while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.n2" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IRS7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065602.1">
        <property type="protein sequence ID" value="ENSPNAP00000040732.1"/>
        <property type="gene ID" value="ENSPNAG00000029030.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155239"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070551">
        <property type="term" value="F:endoribonuclease activity, cleaving siRNA-paired mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098808">
        <property type="term" value="F:mRNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035278">
        <property type="term" value="P:miRNA-mediated gene silencing by inhibition of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035279">
        <property type="term" value="P:miRNA-mediated gene silencing by mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000005">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10 12" id="KW-0943">RNA-mediated gene silencing</keyword>
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    <keyword evidence="8 12" id="KW-0810">Translation regulation</keyword>
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        <location>
            <begin position="1"/>
            <end position="30"/>
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        <ligand>
            <name>a divalent metal cation</name>
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        <location>
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        <ligand>
            <name>a divalent metal cation</name>
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            <dbReference type="Proteomes" id="UP001501920"/>
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    <sequence length="868" mass="98734" checksum="4E42B98D235608A1" modified="2024-11-27" version="1">MLQSICLLTVFVRGVVHLSTIFTNITCISGEYVFKPPQRPDFGTMGRTIKLQANFFEMDIPKLEMYHYDIDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGRDKLEVTIPGEGKDRSFKVAIKWVSCVSLQALHEALSGRLPSIPFETIQALDVVMRHLPSMRYTPVGRSFFTPSEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISKLMRSANFNTDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRNKAIATPIQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNVQKTTPQTLSNLCLKINVKLGGVNNILLPQGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQDIIQDLAAMVRELLIQFYKSTRFKPTRIIYYRDGISEGQFHQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQVHQDTLRTMYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IY21</accession>
    <name>A0AAR2IY21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="11">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:11093"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IY21"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078455.1">
        <property type="protein sequence ID" value="ENSPNAP00000044638.1"/>
        <property type="gene ID" value="ENSPNAG00000035761.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
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        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF46">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
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    </dbReference>
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        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 10" id="KW-0520">NAD</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="12">
        <location>
            <begin position="29"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="13">
        <location>
            <begin position="219"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="33"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
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        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
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        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="292"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="372" mass="40657" checksum="EF9AA866117BD5E6" modified="2024-11-27" version="1">GKCKIMKTTKAVALRHVPQTAESMPGKRVCIVGSGNWGSAIAKIIGHNVKASNRFEPLVNMWVFEETVNGRKLTEIINNDHENVKYLPGHKLPKNVIAVPDVTEAVAGADILVFVIPHQFIGKLCDQMKPHIKPRAIGISLIKGIDEGPAGLTLISDIIRTKLEIDVSVLMGANIANEVADEKFCETTIGATNEQNGHLFKELMQTPNFRITVVSESDTVELCGALKNIVAVGAGFCDGLGFGDNTKAAVIRLGLMEMVAFSRLFCKNPVSSATFLESCGVADLITTCYGGRNRRVAEAFVTARKSIAELEAEMLNGQKLQGPQTSAEVYKILQKRSMVDKFPLFVSVYEICFEGKDVKEFIKCLQNHPQHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZE7</accession>
    <name>A0AAR2IZE7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZE7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074736.1">
        <property type="protein sequence ID" value="ENSPNAP00000045070.1"/>
        <property type="gene ID" value="ENSPNAG00000025063.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158973"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000002">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000001">
        <property type="entry name" value="calcium/calmodulin-dependent protein kinase type II subunit gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="13"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="605" mass="67620" checksum="3B9B2315D4CB4837" modified="2024-11-27" version="1">MATTTCTRFTDEYQLYEELGKGAFSVVRRCVKLCTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLLFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKNAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAQEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLVSRNFSVFPLFNTTVVFSAQPQSNSTKNSIVTSPKGNIPSPALVFTSLLLSVLSPSVSFLFILFVSLALLSCLSLSVFVCISAPSKLTDILGCVRRGSGPTAPPDTEGGAPSVPAPPHTPAIQMLLAYMQCLNGEKAWSWCLRVWPLPCVTLWVLCVLCVLCPARKQEIIKITEQLIEAVNNGDFDAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLSKNSKPIHTTILNPHVHLIGEDAACIAYIRLTQFVDSQGRPRSSQSEETRVWHRRDSKWQNVHFHCSGAPAAPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J5Y0</accession>
    <name>A0AAR2J5Y0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform</fullName>
            <ecNumber evidence="5">2.7.1.137</ecNumber>
            <ecNumber evidence="4">2.7.1.153</ecNumber>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Phosphoinositide-3-kinase catalytic alpha polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine protein kinase PIK3CA</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55632"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83416"/>
            <dbReference type="ChEBI" id="CHEBI:83419"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:55633"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol 3,4,5-triphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77137"/>
            <dbReference type="ChEBI" id="CHEBI:83243"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:43397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:21293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS and KRAS. Interaction with HRAS/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Interacts with FAM83B; activates the PI3K/AKT signaling cascade.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="5"/>
    <dbReference type="EC" id="2.7.1.153" evidence="4"/>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_037397294.1">
        <property type="nucleotide sequence ID" value="XM_037541397.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397295.1">
        <property type="nucleotide sequence ID" value="XM_037541398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J5Y0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079484.1">
        <property type="protein sequence ID" value="ENSPNAP00000047428.1"/>
        <property type="gene ID" value="ENSPNAG00000002579.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413670"/>
    <dbReference type="GeneTree" id="ENSGT00940000164913"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005943">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005944">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IB"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046934">
        <property type="term" value="F:1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08398">
        <property type="entry name" value="C2_PI3K_class_I_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00872">
        <property type="entry name" value="PI3Ka_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000001">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000041">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.770:FF:000005">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000055">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000007">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000593">
        <property type="entry name" value="Zgc:158659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003113">
        <property type="entry name" value="PI3K_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF95">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 3-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02192">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00794">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00143">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51544">
        <property type="entry name" value="PI3K_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51546">
        <property type="entry name" value="PI3K_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K-ABD" evidence="25">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K-RBD" evidence="27">
        <location>
            <begin position="187"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2 PI3K-type" evidence="28">
        <location>
            <begin position="332"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="26">
        <location>
            <begin position="518"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="24">
        <location>
            <begin position="766"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00065166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1070" mass="123667" checksum="D3E7AF5C7B6B9E99" modified="2024-11-27" version="1">MAPRPSSGELWGLHLMPPRILVDCCLPNGMLVSLECLREAPLISVKQQLFTEARKYPLYHLLQEESCYIFVGVTQEAEREEFYDETRRLCDLRLFHPILKVIEPLGNREEKILNREIGFAIGMPICEFELVKDPEVQDFRRSILSVCREAMEEREGGGAHTQALYVYPPSVESSPQLPQHIYCKLDKGRLIVTIWVVVSPSNAKQKYTLKISHDCLPEQLIAEAIRKKTRSMHLSAQQLRLCVQEYQGQYILKVCGCDEYLLEKYPLSQYKYIRSCITVGKLPHLMLVSKDSLYSQLPASGFVAPSYSRRTPQPSPCPGGADPSPPRSLWAFNTLLRVRLLCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLTYDIYLTDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNVNLFDYKDTLVSGKVALSLWPVPHGLEDLLNPIGVAGSNPNKETPCVELEFSSFNQMVVFPDEQQIEEHANWIISRELGYNYSLGLSNRLACDSTISQAELEQLRSLCSRDPLYELSEQEKDFLWRHRHYCVNIPECLPKLLLSVKWNSRDEVSQMYCLLRDWPLMQPESALELLDCNFPDPMVREFALRCLIQGLTDDKISQYLLQLVQVLKYEMYLDNPLARFLIKKALTNQRIGHFFFWHLKSEMHNKTVSRRFGLLLEAFCRGCGMYLKHLNRQVEAMDKLVNLTDTLKHEKKDETQKTQMKFLVEHMSRPDYMEALQGFVSPLNPVHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFTNNEVIFKNGDDLRQDMLTLQIIKIMENIWQNQGQDLRMLPYGCLSIGDCVGLIEVVKNSFTIMQIQCKGGLKGALQFNSNTLHHWIKDKNKGEAYDKAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKENGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGVQECTKTKEFERFQEMCYKAYLAIRQHASLFINLFSLLLGCGMPELQSFDDIAYLRKTLALEKSQQEALEYFTKQMNDAHHGGWTTKMDWIFHTIRHMPNEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J9L9</accession>
    <name>A0AAR2J9L9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J9L9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073166.1">
        <property type="protein sequence ID" value="ENSPNAP00000048733.1"/>
        <property type="gene ID" value="ENSPNAG00000036455.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163630"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="145"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="436" mass="49657" checksum="1AC448B916FF5D79" modified="2024-11-27" version="1">LCGSVLTHFVWESGYFEFGDKPQRLLARQLRGFQANRAIHQIKSPSGVLITDPIAINDCFREYYEKLYQSKAKGDVKTWLKNFKLPKLDEAAQRALDSELTSEEIIDSIKSFPSAKAPGPDGFGIEFYKKFASQITPLLLRMFIHSTKVGTLPKTLYAANISLLLKPGRDKTDFSSYRPISLLNTDLKILSKVLANRLKQYISSIIHPDQTGFIPNRFSFFNVRRLMDIIYHKYHGVSAQAVLSLDAEKAFDQVEWGYIIATLKEFGFGHAFISWIQMLYAHPTASILTNQEQSPYFSLYRGTRQGCPLSPLLFSIAIESLAISIREDENIKPIILGGVEHKLSFEISGYTINWQKSEFMALSANHNPRFLSSLQFKMSTVKIKYLGIEVTKNPNALFKHNFAEKMNRLKQDIDRWRTLPLSLIGRVNAIKMVSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JAD5</accession>
    <name>A0AAR2JAD5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CXXC-type zinc finger protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CpG-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">PHD finger and CXXC domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JAD5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078013.1">
        <property type="protein sequence ID" value="ENSPNAP00000046801.1"/>
        <property type="gene ID" value="ENSPNAG00000006542.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111044"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15553">
        <property type="entry name" value="PHD_Cfp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000138">
        <property type="entry name" value="CXXC-type zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022056">
        <property type="entry name" value="CpG-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF5">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12269">
        <property type="entry name" value="CpG_bind_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="45"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="18">
        <location>
            <begin position="136"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="245"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="364"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="279"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="589" mass="69077" checksum="10A64689B3D8B556" modified="2024-11-27" version="1">MFRFTHTHTHTHTHTHTHTHTHTHTQKYELMHKPHNEGSINQNAPLYCICRKPDINCFMIGCDNCNEWFHGDCIKISEKTAKTIRVWYCERCRSKDDSLEVKFRPKKSREKDAEADGVDSQADTDTPDFKTGRRGSRVKRSARMCGECEACRRTEDCAQCDFCKDMKKFGGPNKIRQKCRLRQCEVRARKMLRVKDEEYSASKSPHMPQRDCPLSDEFSEDELELYEQYRAGRYGDHSWNSDEDDGTCLDPAMRKRAVKVKHVKRREKRSEKKKEENKPRKHKPKQKHRDKVRHSERTEARDSGGTRQCLGPSCVEAARPNSKYCSDDCGMKLAANRIYEILPQRIQQWQQSPCIAEEQGKKLLERIRREQQSARLRLTEMEKRFHELEGIIAKAKQQVVDHDEEVNEGDDDTDLQIFCVSCSHPVNPKVALRHMERCYAKYESQTSFGSMYPTRIEGATRLFCDVYNPQSKTYCKRLQVLCPEHSRDPKVPADEVCGCPLVRDVFEPTGEYCRVSKRKCNKHYCWEKLRRAEVDLERVRVWYKLDELFEQERNVRTAMTNRAGLLALMLHQTIQHDPHATDLRSGKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JBA9</accession>
    <name>A0AAR2JBA9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TEA domain family member 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JBA9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073179.1">
        <property type="protein sequence ID" value="ENSPNAP00000049235.1"/>
        <property type="gene ID" value="ENSPNAG00000035912.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182956"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000001">
        <property type="entry name" value="Transcriptional enhancer factor TEF-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016361">
        <property type="entry name" value="TEF_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002603">
        <property type="entry name" value="TEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00554">
        <property type="entry name" value="TEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="9">
        <location>
            <begin position="50"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="7">
        <location>
            <begin position="50"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="448" mass="49941" checksum="2CC71C5B71A341DA" modified="2024-11-27" version="1">MPLAGSCLQSSPVLGLEVPAVAMDPGSWSGSESGGEDIERMSDSADKPLDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSRELQAKLKVTNLDQSVKDKALQSMAAMSSAQIVSATAIHNKLGLAGLPQPSFPGAPGFWQLSTGHPGSSQDVKPFAQQAYPIQTATAISGFEPPTAPTASPPAWQGRSIGTSKLRLVEFSSFLEQQRDPEAYNKHLFVHIGQTDHLHIDPLLESVDIRQIYDKFPEKKGGLKDLFAKGPQNAFFLVKFWADLNCNLQEDSGAFYGVTSQYESPDNMTITCSTKVCSFGKQVVEKVETEYARFENGRFVYKINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRETQETLLCMACVFEVSNSEHGAQHHIYRLVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JF96</accession>
    <name>A0AAR2JF96_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Probable phosphatase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CTDSPL2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037398936.1">
        <property type="nucleotide sequence ID" value="XM_037543039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JF96"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067600.1">
        <property type="protein sequence ID" value="ENSPNAP00000048516.1"/>
        <property type="gene ID" value="ENSPNAG00000013283.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411888"/>
    <dbReference type="CTD" id="558181"/>
    <dbReference type="GeneTree" id="ENSGT01040000240503"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000015">
        <property type="entry name" value="CTD small phosphatase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="6">
        <location>
            <begin position="287"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="72"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="222"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="470" mass="52925" checksum="5A873C238D51B1A6" modified="2024-11-27" version="1">MRLRVRKASQQLNPTPPVRTTKAKRKHSEVEHTRPSGGAKMQKSDTGIFSTIKRFIRGNAVKVEQCTPAKRSRVDCDSDSNLITSTPPAGNLPSRTNPRTRRKGPANGDTMACESKLVKPNGKLEVQTEAPSSPPRTTLLGTFFSPVFNFFSPANKNATPGSDSPGQAVEAEEIVKQLDMEQLEETPTSTTTDGSRDITFDPALNSRPLLPTETAVEEGEIATEADMPPLTAVGSNSNYPDAPPSPPAEGTYEEDWEVFDPYFFIKHVPPLTEEQLTRKPALPLKTRSTPEFSLVLDLDETLVHCSLNELEDAALTFPVLFQDVIYQVYVRLRPFFREFLERMSQIYEIILFTASKKVYADKLLNILDPKKQLVRHRLFREHCVCVQGNYIKDLNILGRDLSKTIIIDNSPQAFAYQLSNGIPIESWFVDKNDNELLKLVPFLEKLVELNEDVRPHIREKFRLHDLLPPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JGV5</accession>
    <name>A0AAR2JGV5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TTC9C</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein light chain Tctex-type family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JGV5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057469.1">
        <property type="protein sequence ID" value="ENSPNAP00000051185.1"/>
        <property type="gene ID" value="ENSPNAG00000033939.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160019"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF55">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19847" checksum="7B8865529A55D114" modified="2024-11-27" version="1">MEPRSERRLSLGRGRRESLWGGRRESLWGGRRESLWRGQEEEDSAQAGWRGGLRPKLANTYRLEPLHPFAPHLLRQSAEELMGAFGDLQYQQETCRRLARRVADQLLALAKEQEFDRYRYAVQVSVGQKLGQGVKLASKALWDHEKDSFITLRYENTYLFAVGTVFALYMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JJ75</accession>
    <name>A0AAR2JJ75_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cellular communication network factor 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CCN6</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037393517.1">
        <property type="nucleotide sequence ID" value="XM_037537620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JJ75"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070955.1">
        <property type="protein sequence ID" value="ENSPNAP00000052088.1"/>
        <property type="gene ID" value="ENSPNAG00000026744.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415047"/>
    <dbReference type="GeneTree" id="ENSGT00940000160119"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017891">
        <property type="entry name" value="Insulin_GF-bd_Cys-rich_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF3">
        <property type="entry name" value="CELLULAR COMMUNICATION NETWORK FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00222">
        <property type="entry name" value="IGFBP_N_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043501742" description="Cellular communication network factor 6" evidence="7">
        <location>
            <begin position="21"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="9">
        <location>
            <begin position="23"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="8">
        <location>
            <begin position="242"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="36180" checksum="E98A217C89E23245" modified="2024-11-27" version="1">MLSLFYSCLLLLLSQHACRGQADLQLCSWPCKCPAVRPQCAPGVSAVLDGCGCCKSCARQIGEPCNERDVCDPHKGMYCDFSRDKPRFQNGICAYLMAVGCDMNGAHYDNGQSFQPNPLYKCVCIAGAIGCTPAFIQKPAAMLSPAGLQSSAPAGLKAKHQQDTTYRTMSAYRDPPLAWKKNCLVQTAPWAPCSRTCGIGISVRISNDNGKCEMRKERRLCLLRPCEKKTLKNVKIPAGKTCKPTFQARKAEKLSLSGCTSIKKYRPTFCGVCTDRRCCVPNQSRAVQVQFRCTGGATVNWKMQWITSCVCQRRCDEPGDMFSELYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JKU7</accession>
    <name>A0AAR2JKU7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein-lysine N-methyltransferase SMYD4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">SET and MYND domain-containing protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SMYD4</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protein-lysine N-methyltransferase. Monomethylates PRMT5, modulating its transcriptional activity. May also act as a histone methyltransferase. Plays a critical role in cardiac development. Acts as a key epigenetic regulator of gene expression during cardiac development via its dual activities as a methyltransferase and negative regulator of HDAC1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysyl-[protein] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51736"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JKU7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042891.1">
        <property type="protein sequence ID" value="ENSPNAP00000050824.1"/>
        <property type="gene ID" value="ENSPNAG00000022974.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111079"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10536">
        <property type="entry name" value="SET_SMYD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052097">
        <property type="entry name" value="SET-MYND_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044421">
        <property type="entry name" value="SMYD4_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46165:SF2">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="17">
        <location>
            <begin position="231"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="18">
        <location>
            <begin position="290"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="363"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="809" mass="89091" checksum="793C37DB8844539D" modified="2024-11-27" version="1">SSRASAAHSIKSFHVWSPYYFSLSVCNICLSLFSKIKVCKHVTFVGGDLLGFVFRAVIWVVRGGPAVAVSQLPAFSSQWTDICLLSGKPGFDVQEDLEVLSRISERCPVKKSAECAARFREQGNLSFKIKDYRSAALYYSKAACHAGKSTEQLSLCYANRSAALFHLGLYSKCLEDVQRALDEGYPSHLWHKLTVRRTQCLNRLKEQEQPKAPSSKHQTPQTADKKDGNIRPPAVRFTPEKGRHLLVAEDKSAGEVVLEDEAFSFVLIPGDGHDTKTDKACVFGTEHRYCHHCLCLTLSTVPCVGCSYAQYCGQRCASEAWRQYHCLECPAGSELLALGVLAHLALRAALKAGLKEVQRARHRYSNTDDVPTSACVRSDTSKDKPASHSPTSHSDLNVEESSRFSDHSNCYHGSSYLGIYSLLPHVMNHPPSLRFLLAFTMVALSQRLNEVGSFPEAWSCREQEGELGDWREEQSMLGATALRHMMQLRCNAQAVTAVRVKEDGGLAVQSSGEVRIATAVFPVLSLLNHSCCPNTSVSFSLGLSSEAPVSSASGVAVTIRTCKDVAAGQELLHCYGPHCSRMDVGQRQRLLLEQYFFHCHCEVCKMELTAGTRTLATASHGLRCGRCGSSLQARAEVHVCPQSPCNYQISNTELQGRIETLQQRLGRAVQLMENDGLDGALRVLQEAATQADSILMKTHPLQGELADATARVFAALGNWRQAASHLKRSVAAVRCQFGEDSVELGQQLFKLAQLYFNGRDMQSALSVIPSAHRLLSLHCDPHCEELQELQQMEACLRGAVQREDVLTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JN95</accession>
    <name>A0AAR2JN95_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JN95"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061251.1">
        <property type="protein sequence ID" value="ENSPNAP00000053493.1"/>
        <property type="gene ID" value="ENSPNAG00000034103.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287339"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="36156" checksum="C8C4A17B6A3B5DCF" modified="2024-11-27" version="1">MQGIDYMQCIHYIVLTIRSVLTIRSVLTIRSVLTIRSVLTMCSVLTIRSVLTICRVLTIRSVLTMCRVLTICSVLTIRSVLTIRSVLTICSVLTIRSVLTICRVLTIRSVLTIRSVLTIRSVLTIRSVLTIRSVLTIRSVLTMCSVLTIRSVLTICRVLTIRSVLTMCRVLTIRSVLTRRSVLTIRSVLTIRSVLTICSVLTIRSVLTICILTIRSVLTICSVLTIRSVLTICSVLTICMVLTIRSVLTICRVLTIRSVLTICSVLTICRVLTIRSVLTICSVLTIRSVLTICRVLTICLNYLTLRSFIDRWRDNLKCTQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JV79</accession>
    <name>A0AAR2JV79_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor 25 (basic helix-loop-helix)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TCF25</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JV79"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077215.1">
        <property type="protein sequence ID" value="ENSPNAP00000054109.1"/>
        <property type="gene ID" value="ENSPNAG00000004300.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005563"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="654"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="734" mass="84489" checksum="881624872D8714D4" modified="2024-11-27" version="1">MSSRALRRLKGKQRGQEAIDIGDLKLNSEEAGGAELEEEKDEELDEVLEPAKASSSRRAKKSKSQKNISNIYELIGEIDNDVDTNPTDEESVLAARSPEESRVSPELPKQETETPKSDGDRKLNNKKKKKRKKKKASAGDVQEESGAGDDIDALLETIKKTNGLSHTSEDCGAPGYRPVLYVEHRNLNSENELKRYFGARAVLGDQKPRQRQRQFHRNTWMTTPKDTWPRFSRPGISMSLLESKDGLQFFVFEHSRDYQQVQFKFFDAVESMDPNNIVVLLQLNPYHVDSLLQLSDVCRIQEDQETARDLIERALYSFECAFHPVFSLTSGTSRLDYRRPENRAFYLAVYKHMMFLEKRGCPRTALEYCKLILSLDPDNDPLCMLLLIDFLCLRCREYASLIRLYEEWKVHRNLSQLPNFAFSVALACYHLSQQEETTSDESQRMKVKSDIMLQEALIMFPGVLMPLLDLCTVQPDAAVSSHAFFGPKSQLEGLYVGRCHSLWKEAGVMLWMEGNVREVLKRVDSQDPLVDDCQNKRKQRYQSAPRSIHRHVLLSEIKEATSTLPLEVTTQPVMSYDPLPPLDSIMSYTRPERYCTLQVTLFFLDNAIVKYKWGNLSGHRGELFLIGQETLTGEKKEVQQDNNPRHKMWCLNTYFLFLYNEVIIIIIIILSLYKDRLKYIMILDLTPTVRITSTHAVITFPCSVNLCVKQQPRHNLELVWNSGLVIFVNEKFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JX05</accession>
    <name>A0AAR2JX05_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JX05"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049345.1">
        <property type="protein sequence ID" value="ENSPNAP00000056598.1"/>
        <property type="gene ID" value="ENSPNAG00000032798.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164296"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF3">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="240"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="3">
        <location>
            <begin position="481"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="602" mass="68288" checksum="3E6291DEEB5A351F" modified="2024-11-27" version="1">MSLPLNVIQKKAECSDEERQNIWMTSITDRYKRRPKTEEFAKMCLATFASEYRILSKSEGSSTNSIKLDKGLGFVKKRTRTDAAVILYARFSPTKDPEKYYQSILQLFLPHYLESQLKPSYFNTYQEFYETGYVKFCDELCSVKLIVDSNRAMFEKEADIIDQAQEDLEMHGPMEDAWAEICPESEGERLECLASRSDQTTDMDEHSDVIPDLLPKSTCTWQDNPHCMPKQQAMILIRALNEKQSEIFYKIRQWCLQKTRGENPEPFQVFISGPGGVGKSMLIKALHYEAYRILSHLSDNPDEIHVLLTAPTGVSAYNIDAATIHTCLSIGKDIKLPYQPLGDDKINSLRSKLGNLQILIIDEISMVDHKLLAYVHGRLRQIKQAGDYSPFGKVSIIAVGDFYQLGPVKGKPLYIEPPGVNLWQNHFALAELTDIMRQKDAEFAQLLNRLRKRKKGEVLPEQDINMLKQKHGHHATVHNTCLTQALHIGVNARVMLLKNIDVSDGLVNGAFGTVNEVSGKALRAKHPGLKPGLTKATSIQPEEERVTNNGGIRRQFPLKFYKPVIETAPSQSSYKILLHNVQGLTCHIEDIRHMTGGTWWLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JXX5</accession>
    <name>A0AAR2JXX5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Class II aldolase/adducin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldolase class II family. Adducin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037390437.1">
        <property type="nucleotide sequence ID" value="XM_037534540.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390438.1">
        <property type="nucleotide sequence ID" value="XM_037534541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JXX5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045953.1">
        <property type="protein sequence ID" value="ENSPNAP00000056908.1"/>
        <property type="gene ID" value="ENSPNAG00000018374.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440689"/>
    <dbReference type="CTD" id="556762"/>
    <dbReference type="GeneTree" id="ENSGT00940000155257"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000004">
        <property type="entry name" value="gamma-adducin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF5">
        <property type="entry name" value="GAMMA-ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="12">
        <location>
            <begin position="139"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="465"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="527"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="569"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="589"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="631"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="666"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="690" mass="77396" checksum="4E40E9747521CE8E" modified="2024-11-27" version="1">MSREVSAGVVTTPPPPSVGQKERYFDRINENDPEYQRSRNMSPDLRQDFHMMEQKKRVTQILQSPAFKDELEGLIQEQMNKGNDPAGLLALRQIADFFMTSSIAGFSTSPLSLGMVTPINDLFSTESSNMVKGEKQTRCKLASLYRVVDLFSWAHFANSYITVRVSKEQDHILIIPRGLSFAEASASNLVKVNILGDVVDQGSTNLQIDPSGFSPHAAIYSMRPDVRCIIHIHTPAVAAVSSMKCGLLPISQESLILGDVAYFNYQGSLDEQEERVELQKALGPSAKVLVLRNHGVVALGETIEEAFHYTYTAQLACEIQVNAFSCAGGVENLIVLDLEKYRSRTQGVAEVGVNLGSHHKWKIGELEFESLMRMLDNLGYRTGYAYRHPIVRDKPRHKSEVEIPATVTAFSFDEDEVPRLPFKLLQQRQQREKTRWLNSPNCYTKVNVADGSSEESVRVRTMWMKSEDGSSSSGTPIRIEDPNQFVPLNTNPSEVLEKRNKIREQNRYDMMSAGPQSQKLAGIVVERPPEPSSAKPYTGGDEDQTEPLPPNPFSELTERELNDYKKRVERQQLGLDEEEQDQLTSDDASTLSQSVSQTQSPQNTPAKEENHTNILLNGKDDHGEEEELSKRMSQLTTSVESVEITVRSGEKIEEALSPESSPSKSPNKKKKKFRTPSFLKKNKKKEKVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K4R0</accession>
    <name>A0AAR2K4R0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Follistatin-related protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Follistatin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation. Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter. Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes. Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with SCN10A. Interacts with DIP2A; DIP2A may act as a cell surface receptor for FSTL1. Interacts with BMP4. Interacts with CD14; this interaction promotes TL4-mediated signaling cascade.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K4R0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054055.1">
        <property type="protein sequence ID" value="ENSPNAP00000059255.1"/>
        <property type="gene ID" value="ENSPNAG00000006423.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157784"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030510">
        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16233">
        <property type="entry name" value="EFh_SPARC_FSTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000274">
        <property type="entry name" value="Follistatin-like 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057020">
        <property type="entry name" value="EF-hand_FSTL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015369">
        <property type="entry name" value="Follistatin/Osteonectin_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF13">
        <property type="entry name" value="FOLLISTATIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23564">
        <property type="entry name" value="EF-hand_FSTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09289">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23244">
        <property type="entry name" value="VWF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="14">
        <location>
            <begin position="58"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="315" mass="35748" checksum="FCF3B2139EED533C" modified="2024-11-27" version="1">MLLTGFDVTWILRSRKCNQLLRLNFGGETQSKSKVCANVFCGAGRECAVTEKGEPSCLCIEQCKPHKRSVCGSNGKTYRNHCELHRDACLTGLKIQVAHDGHCKGERGAMSFIYLVCYVADRNELRRRVIEWLQTEVVPDGWFTKGSNFTDILLKYFKNYDNGDSQLDSSELLRFIQHNETAVELRSYAEEENNRLLRSLCVDALIELSDQNADWKLSFDEFLNCLRPGFNPPDKKCALEDETYEDGAETQVECNRCVCACGNWVCTAMTCDEKSPAPEAAAGDEMTEEEWSLRVAELNKHQETMEKMKSSTKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K7T1</accession>
    <name>A0AAR2K7T1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">COPA</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with SCYL1. Interacts with JAGN1. Interacts with TMEM41B. Interacts with SVEP1. Probably interacts with PEX11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Golgi apparatus membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K7T1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045014.1">
        <property type="protein sequence ID" value="ENSPNAP00000058156.1"/>
        <property type="gene ID" value="ENSPNAG00000010849.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155451"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000010">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016391">
        <property type="entry name" value="Coatomer_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF1">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003354">
        <property type="entry name" value="Coatomer_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="5 16" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="201"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="17">
        <location>
            <begin position="341"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="19">
        <location>
            <begin position="657"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="18">
        <location>
            <begin position="861"/>
            <end position="1270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR003354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1270" mass="143454" checksum="021D0068700CFEE7" modified="2024-11-27" version="1">MLTKFETKSARVKGLSFHPKRPWILASLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFHKQQPLFVSGGDDYKIKVWNYKLRRCLFTLLGHLDYIRTTFFHHEYPWLLSASDDQTIRIWNWQSRTCVCVLTGHNHYVMCAQFHPSEDLVVSASLDQTVRVWDISGLRKKNLSPGAVETEVRGITGVDLFGASDAVVKHVLEGHDRGVNWAAFHPSMPLIVSGADDRQVKIWRMNESKAWELDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVWDMSKRTGVQTFRRDHDRFWVLGAHPNLNLFAAGHDSGMLVFKLERERPAYAVYGNMLYYVKDRFLRQLDFNSSKDTAVMQLRSGSKFPVFSMSYNPAENAVLLCTRATNLENSTYDLYSIPRDSDSQNPDAPEGKRSSGLTAVWVARNRFAVLDRMHSLLIKNLKNEIVKKVQVPSCEEIFYAGTGSLLLRDADGVTLFDVQQKRSLATVKIAKVKYVVWSADTSHVALLAKHGTAATALHFKNSQLYINNNVMSYLRWFQKMFNVMLSSKIMETVLLVSIMICNRKLESLCNIHENIRVKSGAWDESGVFIYTTSNHIKYALTSGDHGIIRTLDLPIYVTRVRGNSVYCLDRECRPRVLNIDPTEYRFKLALVNRKYEEVLHMVRNAKLVGQSIIAYLQKKGYPEVALHFVKDEKTRFSLALECGNIEVALEAAKALDERSCWERLGEAALLQGHHQVVEMCYQRTKNFDKLTFLYLITGNLAKLRKMMKIAEIRKDMSGHYQAALYLGDVSERVRILKNCGQKSLAYLTAATHGMDEEAEALKETFDLEKEMIPEVDTNAQLLQPPPPINPLDTNWPLLTVSKGFFEGAIAAKGRAGQMAADLDMDTPGGEGWGEDAELHLDDDGFMDAQEGLGDEGGVGKEEGGGWEVEEDLDLPPELDVPAGAGGGAEGEGFFVPPTKGVSPAQMWCNNSQLPVDHLLAGSFETSMRLLHDQVGVVQFGPYKQLFLQTFSRGRTCYLGLPSLPCLQGHPQRNWKDSGLKAGLPAVGLRLADLIARLQQCYQMTTAGRFEEAVERFRVILLSVPLLVVDNKQEIAEAQQLITICREYIVGLTMETERKKLPKDTLEQQKRLCEMAAYFTHCNLQPVHMVLVLRTALNLFFKLKNFKTAAGFARRLLELGPKPEVAQQTRKILAACEKTLTDAHQLNYDPHNPFDLCAASFTPLYRGRPVEKCPLSGACYCPTYKGQVCRVTQVTEIGKEVIGLRISPLQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KC30</accession>
    <name>A0AAR2KC30_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Striatin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">STRN</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26. Interacts with CTTNBP2; this interaction may regulate dendritic spine distribution of STRN. Activation of glutamate receptors weakens the interaction with CTTNBP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat striatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KC30"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048471.1">
        <property type="protein sequence ID" value="ENSPNAP00000061835.1"/>
        <property type="gene ID" value="ENSPNAG00000010727.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183095"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070016">
        <property type="term" value="F:armadillo repeat domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.300:FF:000001">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000058">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000079">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000211">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF2">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 14" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="17">
        <location>
            <begin position="42"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="472"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="525"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="578"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="713"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="258"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="377"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="60"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="791" mass="87284" checksum="CD4ABB23752451F8" modified="2024-11-27" version="1">MDEHAGPGVFFNNNNNSLLQAGAKGAAAESDGGEAGRAQYSIPGILHFLQHEWARFEVERAQWEVERAELQAQVAFLHGERKGQENLKKDLVRRIKMLEYALKQERAKYHKLKYGTDLNQGDMKPPSYDSDEGNEKETSGSLNNQLSWKQGRQLLRQYLQEVGYTDTILDVKSQRVKALLGLAGDGSGDRSSQEPLLNGTDTAPKGANTAGKVELSDSSTVLEVFKFIESAAAEFSDEDDEEDSEGKEKSIIDSRTIVRKKPSSFSPTHASDDPDTEDALKGFDFLSNPEDMDTSPESRSAGDGTDWEKDRQAWDVDQSLITKLKEQYRKERKGKKGVKSKWTWPPLSSPFSSSLSLNLKKIKNALQDMLANLRDTEEPSSLQPSIAPPPRPNANRFNEHDSSNTDEVEAITFPPSSGKAFIIGTDEAMETGELGLGELAGLTVANEADNLTYDITNNKDALRKTWNPKFTLRSHFDSIRSLAFHPVDPVLITASEDHTLKMWNLQKTTPAKKNASLDVEPIYTFRAHRGAVLCVVMSNTGEQCYSGGEDGTIQCWNTPSPNIDPYDSYEPSVLRGALCGHADAVWGLVHSSAHQRLLSCSADGTVRLWNASNPQAALAVFNENKELGLPSSVDLVSSEPAYMVTSFTSGRIGLFNMETRQLVLNLESTVEPGASCQINKVLSHPTLPITITAQEDRHIKFFDNNTGKLIHSMVAHLDAVTSLAVDPNGLYLMSGSHDCSIRLWNLENKTCIQEFTAHRKKFDESIHDVAFHPSKCYIASAGADSLAKVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KFP1</accession>
    <name>A0AAR2KFP1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KFP1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060162.1">
        <property type="protein sequence ID" value="ENSPNAP00000063133.1"/>
        <property type="gene ID" value="ENSPNAG00000013499.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160876"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000293">
        <property type="entry name" value="zinc finger protein 385B isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051845">
        <property type="entry name" value="Znf385"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF13">
        <property type="entry name" value="ZINC FINGER PROTEIN 385A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="251" mass="27481" checksum="140491617F12C0CB" modified="2024-11-27" version="1">KEFKDFKDCSTPLHVKALSTMGTPTSMVHGSHSSASGQVECGVEKPCASPALSTDRSEEGKVNTRLFCYLCKIAVNSPSQLQEHNRGTKHKTILEARSGLGPIKAYPRLYFSSNPGHSAQERTFHCEICNVRVNSEPQLKQHISSRRHRDAVAGKSNPLVGHHKSLGSAELMYTLLFHKDLSKSFNYGLLPNTMAVAAAATSSNQLALCTAGPSPHLYLPIIHTTQLNPTLLRPALRSIHSSHKPVFFSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKB6</accession>
    <name>A0AAR2KKB6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E3 SUMO-protein ligase NSE2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E3 SUMO-protein transferase NSE2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Non-structural maintenance of chromosomes element 2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NSMCE2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571486.1">
        <property type="nucleotide sequence ID" value="XM_017715997.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKB6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078372.1">
        <property type="protein sequence ID" value="ENSPNAP00000062551.1"/>
        <property type="gene ID" value="ENSPNAG00000032764.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108438271"/>
    <dbReference type="CTD" id="286053"/>
    <dbReference type="GeneTree" id="ENSGT00940000164859"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="14">
        <location>
            <begin position="138"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="229" mass="25796" checksum="6D49773107011D1C" modified="2024-11-27" version="1">MSLSSIQPTLSTLKTCQVDIGTGMDIVSDVALDLVEAQGTDDSPSLRKLEQMMLECATLDREINCFVESVEQMTARVRHEPPHAMFTLLDSVKDRFSELMAEDADIQRHSKVVAFRDSIRKSVSQGNQMAAANVEEELDEDIAVTQSQTNFTCPLTQVEMVNPVRNKKCQHYYDQEAALAMIKTKHNNKKKFRCPVVGCGNMDVKQSDLELDLVMKRMIQNHKRQSGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNG0</accession>
    <name>A0AAR2KNG0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.22" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNG0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074403.1">
        <property type="protein sequence ID" value="ENSPNAP00000065840.1"/>
        <property type="gene ID" value="ENSPNAG00000012345.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157938"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004398">
        <property type="term" value="F:histidine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042423">
        <property type="term" value="P:catecholamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001694">
        <property type="term" value="P:histamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006548">
        <property type="term" value="P:L-histidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF68">
        <property type="entry name" value="HISTIDINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="467"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="467"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="702" mass="82368" checksum="3733FFFD64D60702" modified="2024-11-27" version="1">MQAEEYTRRGKELVDYIGQYLTEIRSRRVLPDVQPGFLRTLLPSSAPCEPEPWENILQDVENIIMPGVVHWQSPHMHAYFPALNSWPSLLGDMLADAINCLGFTWASSPACTELEMCVMDWLCKALGLPDHFLHHHPDSRGGGILQSTVSECTLVALLAARKDKILQMKSSDSTEMLSDSLTDTDESILNSRLIAYASDQAHSSVEKAGLISLVKIRFLETDEKFSLRGETLQRAIQEDRRRGLLPVMLCATLGSTGVCSFDCLAELGPICAREGLWLHVDAAYAGSALLCPELRYFLSGMEFADSFVFNPSKWMMVHFDCTAFWVKDKFKLQQTFSVDPLYLRHDNSGATDFMVQTHTHTHTHTHTHTKTHRHTHTHKYTHRHAHTHTHTHTHTQRHTDTHAHTQTHTHAHTHKDTHKYTHTYTQTHTRTHTQIHTHVHTHTHTDTHKDTQTRTHTHIHIHLYIHTQRHTHRHTHTHKDKQTHTRTNTHTQTHTHRHTHTHTFRYTRTKTHRHRDTDTHIYTQTQTHIHTHTHRHTHKDTKIHTKTHTHTHIYTHRHRHIHTQTHIYTHRHTHTHTHTHTQRHTHTDIHKLTHRYTHTHTHIQTHTLHTHPHTHTYRHIHTYTHIDIYIHTTHTVSFSHTHTLKYIHTLTHTHTDIYTHIHTHRRIYIHTTHTVSFSHTHTLKYIHTLTHTHTDIYTHTHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KPJ1</accession>
    <name>A0AAR2KPJ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CLASP2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Centromere</location>
            <location evidence="4">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CLASP family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000049526.1">
        <property type="protein sequence ID" value="ENSPNAP00000066200.1"/>
        <property type="gene ID" value="ENSPNAG00000027362.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155574"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902903">
        <property type="term" value="P:regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000001">
        <property type="entry name" value="CLIP-associating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000005">
        <property type="entry name" value="CLIP-associating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000006">
        <property type="entry name" value="CLIP-associating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF30">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="19">
        <location>
            <begin position="8"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="17">
        <location>
            <begin position="167"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="19">
        <location>
            <begin position="309"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="19">
        <location>
            <begin position="847"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="19">
        <location>
            <begin position="1251"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="233"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="533"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1092"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1160"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="629"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="654"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="672"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="700"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1112"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1163"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1188"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1221"/>
            <end position="1234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1496" mass="164180" checksum="F4310AC7339B5029" modified="2024-11-27" version="1">MEENDNMDYFYQQVLQKDVSRRLQVGPDLIDYLSDPQRSADVEQDKSRLDKTIDELTGWVNSSNYKFSVMGLKVQSQGVIPCMLKVFFQCMVPALVDRLGDGKDQVRDQAQALILKLMEQTATPMYIWERLFPGFKHKNFRSREGLCLCLVATLNAYGEQPLSLSKFVPHLCVLTEDQNPQVRETAISTLVEVYRRVGERVRADLGKRGLPAARSARSRSRALSLLLSHRSFDDDDSVDGSRPSSVQAAFKVPKVPKKPGDSASSSRRPSATGAAKTSASKEGAGAVDEEDFIKAFTDVPTVQIYSTRDLEDNLNKIREICSDDKHDWDQRANALKKIRSLLVAGANNYDCFYQHLRLLDGAFKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPVLFNLIPNCAKVMATSGTAAIRIIIRHTHVPRLIPLITGNCTSKSVAVRRRCYEFLDMLLQEWQTHSLERHAAVLVESIKKGIRDADSEARVEARKAYWGLRAHFPGEAESLYNSLEASYQKTLQSCLKSSGSVASLPQSDRSSSSSQESLNRPLSSKWSAAPGRVPASSKSSGSPGSLQRSRSDVDVNAAAGAKARHSGQAGGAGRLTTALPPGSYASLGRLRTKQPLSTPSGVGSSQVDSRGRSRTKMVSQSQPGSRSGSPGRVLASTALSTLSTGAQRVSAVPGSHRRSRIPRSQGCSRDSSPTRLSVARGSRIPRPSVSQGCSREASRESSRDTSPVRSFTPLASRHYSRSTGALHAPDAFGAAGSGLGMSQSSRLSSSVSAMRVLNTGSDVEEALADALKKPARRRYDTYGMCSDDDANSDASSVCSELSYSSRNGSIPTYMRQTEDVAEVLNRCASANWSERKEGLMGLQTLLKNQRTLSRVELKRLCEIFTRMFADPHSKVFSMFLETLVDFIVVHKEDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRESFPNDLQFTILMRFTVDQTQTPNLKVSAAFSSGCVKVAILKYIETLTLQMEPQDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQSVLIALFQLNTPEFTMLLGALPKTFQDGATKLLQNHLRNTGNVAQAPVGSPLTRHTPRSPASWSSPLTSPTNTSQNTPSPSTFDYDTENMNSEEIYSSLRGVSQAIQNFSVRSQEDMTEPPRKREGDGGEEVRGGPDTTDSGRTALDNKTSLLNTMPLLSSSPRPTREYQPGSYSDSSFGSSPFNKSLKDALDQDAESLTDDSNVDQSEVVAELLKELSNHSERVEERKAALCELMRLIRETQLHVWDEHFKTILLLLLETLGDGEHVIRALALRVLKEILNRQPWRFKNYAELTIMKTLEAHKDPHKEVIRAAEEAAAMLASSISPEQCIKVLCPIIQSADYPINLAAIKMLTKVIERLSKDGLLQMLPEIVPGLIQGYDNSESSVRKACVFCLVAIHSIIGEDLKPHLSQLSGSKLKLLNLYIKRAQSGSSGSDPSSDMGGQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KQA7</accession>
    <name>A0AAR2KQA7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KQA7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065469.1">
        <property type="protein sequence ID" value="ENSPNAP00000066460.1"/>
        <property type="gene ID" value="ENSPNAG00000032878.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287822"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="86" mass="10283" checksum="180E1A4506ADB0A9" modified="2024-11-27" version="1">PPWTTHALSIRLTHLRSQSIWPCRYLDTSTPAFAYSIWYCSTRRINHRHETNKAQVLSREVNIICVELEALWKLFLRKAEVAKSWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KRN5</accession>
    <name>A0AAR2KRN5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein Jade-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">JADE3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the JADE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017576277.2">
        <property type="nucleotide sequence ID" value="XM_017720788.2"/>
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    <dbReference type="Ensembl" id="ENSPNAT00000067246.1">
        <property type="protein sequence ID" value="ENSPNAP00000065067.1"/>
        <property type="gene ID" value="ENSPNAG00000002125.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441314"/>
    <dbReference type="GeneTree" id="ENSGT00940000158722"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
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        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PHD_JADE3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Jade family PHD finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein Jade-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039550">
        <property type="entry name" value="JADE3_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF27">
        <property type="entry name" value="PROTEIN JADE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="201"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="253"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="571"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="652"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="723"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="660"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038371"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="803" mass="89954" checksum="45F9847B43282A6F" modified="2024-11-27" version="1">MKRLRTSSSSDSSDNETPSTSFSSNKYGSKPGTPASAQKKPAEVFRKDLISAMKLPDSHHISPEDYYLLADTWRQEWEKGVQVPASPDTIPQPSVRIIAEKPREILFSRQRKYIQCCSQDSTETGYVNIKELAEAMCRYDLDDMDLYWLQELNMELGQMGEGPMNELTMERAMEALERKCHDNMNHAIETEEGLGIEYDEDVICDVCRSPDSEEGNDMVFCDKCNICVHQACYGIVKVPDGNWLCRTCVLGIDPQCILCPKKGGAMKATRAGTKWAHVSCALWIPEVSIACPERMEPITKVSHIPPSRWSLICSLCKLKTGACIQCSVKNCTIPFHVTCAFEHNLEMKTILDEGDEVKFKSYCLKHSKSKAGEPGLSPARHKPPTETEKVGLRAQKLQELEEEFYKLVQPEEVAQDLGLPLHLLDFIYQYWKLKRKTTFNKALLPPKEDEENLLLQPQEDSIHTRMRMFMHLRQDLERVRNLCYMVSRREKLKLSQSKAQEQIFNLHVKLINQELSAGLPVSSAVENLLFRPPPRITLKLKMPKVSLGNKASTKSGNGPLCPDNSSNVYDLSGTGVGQGKPQLHHSRTRREERTNGLLPVARRDSGTPSASPLPLKPTGKPLALHAALHGHSSNGKEKPEQDRPCLMKSNGIMEKPVVQRDTSFQTSSEQATRNEDSGKGRTGSFHKSAIEHFSRSFKEATVSLVRTTEDFWSAEKNARKVVSSKERTNCAKPAIERPQGSSRAYQDSDGYCPDLELSDSEPEAKGKRLRAGRGPSERSSATIYGRGGSRGKVSLGSRSTVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KWD0</accession>
    <name>A0AAR2KWD0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ACYP2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KWD0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063493.1">
        <property type="protein sequence ID" value="ENSPNAP00000068565.1"/>
        <property type="gene ID" value="ENSPNAG00000034777.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="64"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="175" mass="19807" checksum="4AE3C8BC54A720C0" modified="2024-11-27" version="1">MRYVPHTAQANITAERWNRDGALIQSQSSYHTRECVNWLKRFGKTNLDQTGSISFFQRTAFSPVVCHVAGFCPSAMKISWRRDGKDMKENVRIGAALPDGEGTYLRDVSFNITELDSNSFTCVVGNTSRDLDKHQLKVEKSECNQLTPSYISIYCIYTQLQMYAKSFGAPLKGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KZX0</accession>
    <name>A0AAR2KZX0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homeobox protein cut-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the CUT homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037402862.1">
        <property type="nucleotide sequence ID" value="XM_037546965.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000061220.1">
        <property type="protein sequence ID" value="ENSPNAP00000067922.1"/>
        <property type="gene ID" value="ENSPNAG00000005948.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415605"/>
    <dbReference type="CTD" id="797445"/>
    <dbReference type="GeneTree" id="ENSGT00940000159751"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000004">
        <property type="entry name" value="Cut-like homeobox 1a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000010">
        <property type="entry name" value="Cut-like homeobox 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000116">
        <property type="entry name" value="Cut-like homeobox 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057476">
        <property type="entry name" value="Cux_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF4">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25398">
        <property type="entry name" value="CUX1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01109">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51042">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="10 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="16">
        <location>
            <begin position="528"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="CUT" evidence="16">
        <location>
            <begin position="888"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="CUT" evidence="16">
        <location>
            <begin position="1038"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="1168"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="1170"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="244"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="340"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="507"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="614"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="789"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="967"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1238"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="423"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
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            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="832"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
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            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
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            <begin position="979"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1285"/>
            <end position="1301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1337" mass="147846" checksum="57B3ED67D76CF9B2" modified="2024-11-27" version="1">MAANAGSMFQYWKRLDLQQLQKELDATAAALTNRQEENEQSRKKLIDQSRELKKNTPEDLRKQITPLLKGFQTEIDALCKRSKESEAAFLSVYKRLIDVPDPVSALEAAQQLQLTVRKMHDVETENQKLHEKLQEYDRDSAQVKGHEVTIKALQEKLEEFERLFQKQQGLKTEDDEEEEEAEHPPSNYADKDGELQERLNSEEIKLQTLQTALEKTQAELQELKAKYEEESRTKASEIEAMKADMEKANQRAEAAERETESLREQLVNQQQETPDRVCEVECVSDDVQKLQNDLTELTESMANQISRLQQQLSAKEEQLKHLEEKLEAQSDYEELKKELSVLRSMESSSSDQPAAQDPLRPLELLLSGKESSHRDGRSTHSELTGSAGRKDKGSPVESSQRLPATPVPTNTTQGSPPQLLMRTGTATSESAASANGAHPFSPTGIGPDFFTPAVASVGATFPPLAGKMALSSLLQRQLMQSFYTKALQESSPGALLFAPQHYASSPLPPAASPDANGMVPSPSRSEGAGSASEGEELDTAEIARQVKEQLIKHNIGQRVFGHYVLGLSQGSVSEILARPKPWSKLTIRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQNLNRVFQEVPKRRTGSEGSSKPGNITTRIRTPDGGSDEAIKSILEQAKRELQVQKVESVQPLSSSSDENIRSILDHARREMEVQHGALEPGLKAQPEISLLGSKLLTSPLSGLAYSPLALTLKKPLDYHALVKRENSEAALSETSRNSDAGGSWREQWWNTIQSERRGVAQLSNEDAQSQEDSKEKPPRLASTAPPVPGSSLDCWKDWPSAESPFSPFSQSSELSLPMAGGSGSETPQNSPLPSCPLLAIAKTASKPAVAPLTPEQYEQYMYQEVDTVELTQQVKDKLAKNGICQRVFGEKVLGLSQGSVSDMLSRPKHWSKLTQKGREPFIRMQLWLNGENTHQSQVSAEITPKTSASCSPAPESPVSSAEESVKSQLEDPCVQPAGPESSEASESQPDTLLPLPVPGHAGLSIQEMVAMSAEVDTYAITKRVKEVLTDNNLGQRLFGERILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNSVDRLMEMKRLEKKAYMKRQHSSLSESPSREGGVCMGDYSRAVSPQPIVRDHQPQLKKPRVVLAPEEKEALKRAYQQKPYPSPKTIEELANQLNLKTSTVINWFHNYRSRIRRELFIEEIQAAVGGKSGADGESSCDGAESDSRASGLEEPNPDEPAASGHTDALFTFPESSSALASSSASSATPSSNPRDSSLRRKKAANLNSIIHRLEKAANRDEPHEWEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L992</accession>
    <name>A0AAR2L992_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Beta-glucuronidase</fullName>
            <ecNumber evidence="5 12">3.2.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GUSB</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">Plays an important role in the degradation of dermatan and keratan sulfates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:17633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="ChEBI" id="CHEBI:83411"/>
            <dbReference type="EC" id="3.2.1.31"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Inhibited by L-aspartic acid.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.31" evidence="5 12"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L992"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047316.1">
        <property type="protein sequence ID" value="ENSPNAP00000071227.1"/>
        <property type="gene ID" value="ENSPNAG00000020143.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001752"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019391">
        <property type="term" value="P:glucuronoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000027">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000628">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000029">
        <property type="entry name" value="Beta-glucuronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006101">
        <property type="entry name" value="Glyco_hydro_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023232">
        <property type="entry name" value="Glyco_hydro_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023230">
        <property type="entry name" value="Glyco_hydro_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Ig-like_GH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007538">
        <property type="entry name" value="PRK10150.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10066:SF67">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00132">
        <property type="entry name" value="GLHYDRLASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="beta-Galactosidase/glucuronidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00719">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00608">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycosyl hydrolases family 2 sugar binding" evidence="15">
        <location>
            <begin position="36"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 immunoglobulin-like beta-sandwich" evidence="13">
        <location>
            <begin position="228"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 2 catalytic" evidence="14">
        <location>
            <begin position="328"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="653" mass="73748" checksum="E2AB46A3F5CDD12F" modified="2024-11-27" version="1">GLPGGALACLAAVCGLTVCVTGGMLYPRESPSRERKELDGLWAFRADFSPDRSAGFNQAWFKQPLAETGPVIDMAVPSSFNDITQDAELRDFIGWVWYEKEVWVPVRWLQDKDTRIVLRVGSAHYYSVVWVNGVKVVEHEGGHLPFEADIDGVIRKDVGMPCRITIAVNNTLDLHTLPPGTINEILPVNLFLLQIRYPTGYFIQGYNFDFFNYAGIHRSVLLYTTPRAYIDDITVDTNFSDSTGFVFYNVSVVGSAKPAVKVALSGKDGRCVATADGPSGVLKVPEVNLWWPYLMHANAGYLYSMEVQLTAESGQIDVYVMPVGIRTVEVTSSKFLINKKPFYFHGVNKHEDADIRGKGFDWPLIVKDFNLMKWMGANSFRTSHYPYAEEILQMADRHGIVVIDECPGVGIKDIHSFGNESLAHHLVVMEELVRRDKNHPSVVMWSVANEPAAEMPPAAFYFKQLVAHTKSLDRTRPVTFITDSNYARDLGAPYVDVICVNSYFSWYHDPGHLEVIPIQLNTQFDNWYGKYQKPIIQSEYGADAVPGLHNDPPTMFTEEYQKQVLQNYHKVFDQKRKEFVVGELIWNFADFMTSQGITRVVGNKKGMFTRQRQPKSAAFLLRERYWTIANGTAHFSCRFITSGCVHSPEGLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBQ7</accession>
    <name>A0AAR2LBQ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5-oxoprolinase, ATP-hydrolysing</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">OPLAH</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559994.1">
        <property type="nucleotide sequence ID" value="XM_017704505.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017559995.1">
        <property type="nucleotide sequence ID" value="XM_017704506.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000057249.1">
        <property type="protein sequence ID" value="ENSPNAP00000074028.1"/>
        <property type="gene ID" value="ENSPNAG00000012172.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431399"/>
    <dbReference type="CTD" id="26873"/>
    <dbReference type="GeneTree" id="ENSGT00390000013463"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="5">
        <location>
            <begin position="10"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="3">
        <location>
            <begin position="236"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="6">
        <location>
            <begin position="566"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="4">
        <location>
            <begin position="757"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1270"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1315" mass="142756" checksum="044BA25BA513484B" modified="2024-11-27" version="1">MAETRGKFDFAIDRGGTFTDVFARLPDGRERVLKLLSHDPQNYRDAPTEGIRRILEQETGLSFPRDQPLDSSLIGWIRMGTTVATNALLERQGERTALLVTRGFKDLLHIGTQARPNLFDLEVRMPEVLYEEVVEVDERVVLRQDGCQLPRKEPKRIVTGSTGDSLEVWQELDLQRVERDLKAVLSRGITSLAVLLLHSYIWPAHEKAVGSLARKLGFTQVSLSSEVMPMIRAVPRGYTVCADAYLTPKIHQYLNGFTRGFKGALKDVDVLFMQSDGGLTPMEQFSGSRAILSGPAGGVVGYAVTSYNSSENKPVIGFDMGGTSTDVSRYAGQYEHVFEATTAGVTLQAPQLDINTVAAGGGSRLFFRSGMFVVGPESAGAHPGPACYRKGGPLTVTDANLALGRLLPDFFPHIFGPGENEPLSTEETMRQFQQLTHHINHFLSTNQSQHNANGAGQSNSDGPKGCESEMSVEEVAMGFIKVANETMCRPIRALTQAKGHDTSQHVLACFGGAGGQHACAIARALGMKTVFTHKYSGVLSAYGLALADVVEEVQEPCSLQYNEDSYSELDHRIQELTQRCHGTLHQRGFSSSQISTAVFLHMRYEGTDCALMVNTSDYPAHAQSCQSGDFRTAFTKRYMKEFGFTIADRPIMVDDIRVRGSGRSGIKSVTMAMEGHRSPAPTKITKCYFEEGYLDTSVYMWEDLSCGYSIEGPAIIIDKNSTILVEPGCSAQLTSSGDVCIAVGTDSQCVLGTELNTVQLSIFSHRFMSIAEQMGRVLQRTSISTNIKERLDFSCAVFGPDGGLVSNAPHIPVHLGAMQETVQYQLKAIGSSLKEGDVILSNHPCAGGSHLPDLTVITPVFWHGVNGPVFFVASRGHHADIGGITPGSMPPHSTSLQQEGAVFTSFKLVTEGVFQEKAVTEALLDPAQYPGCSGTRNLHDNLSDLRAQVAANQRGSQLVGELIDCYGLAVVQAYMGYIQSNAELAVRDMLKEFARRQQQQTGSLEVEAEDQMDDGTPIRLKVQINEEEGSAVFDFSGSGTEVWGNCNAPRAITLSALIYCLRCMVGQDIPLNQGCLTPIKVIIPPGSILQPSQNAAVVGGNVLTSQRVVDVIFRAFEVCAASQGCMNNVSFGSDKSGYYETVAGGAGAGPGWSGRSGVHSHMTNTRITDPEILEKRYPVILQQFSLRPGSGGRGRYHGGDGVIRKLLFRSKVVLSVLTERRSTRPYGLHGGQSGSAGLNLLHRADGKVLNLGAKTTVGLEPGDMFCLHTPGGGGYGREEEEDEDDNRSLSKRQCLNEAFAERGSVFEYRRTQESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LL41</accession>
    <name>A0AAR2LL41_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">IPO8</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LL41"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046316.1">
        <property type="protein sequence ID" value="ENSPNAP00000075387.1"/>
        <property type="gene ID" value="ENSPNAG00000001408.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158848"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000053">
        <property type="entry name" value="Importin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF26">
        <property type="entry name" value="IMPORTIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="888"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="893"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="912"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="925"/>
            <end position="941"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1040" mass="119978" checksum="7A329E7AFA00536B" modified="2024-11-27" version="1">MDPNRIIQALKGTIDPNLRIAAENELNQSYKIINFAPTLLQIIVSDQVEFPVRQAAAIYLKNMVSQYWQDREPSLGEVVFPFNIHENDRGQIRENMVEAIIQCPESIRAQLTVCLRTIIKHDFPGRWTGIVDKISLYLQSQNSGSWYGSLLALYQLVKTYEFRKAEERAPLLAAMQLFLPRIQQLITQLLPDATVFSVLIQKQILKIFHALVQYSLPLQLLNNTVMTQWMEILRAVVDRDVPPETLEVDEDDRPELIWWKCKKWALHIITRLFERYGSPGNVTKEYFEFADFFLKTYTVGIQQVLLKVVELHRQKQYVSPRVLQQTLNYLTQSVSHSITWKQMKPHMQTITQEVVFPLMCYKDEDEKLWQEDPYEYIRMKFNMFDDHVFPATAAQTLLCKASRMRKEVLPQMMEFCHHILMDPNADPRRKDGALHVIGALADPLLKKRVYRDQMELMLQNYVFPLLNSNLGYLRARSCWVLHCFSPLKFHNELVLRNAVELVKQDLIADQEMPVKVEAAIALQTLVSNQEQAKIYIRPFIRPVMQELLHVIKETENDDLTSVIQKMICEYNQEVAVIAVDMTQNLAEIFSKVLQSEEYEESEDKTVMALGILSTIDTILTVMEDHKEITQQLEGICLQVIGLVLQKPIIEFYEEILSLAFGLTCQTISPQMWQLLGVLYDVFQHDCFDYFTDMMPLLHNYVTVDTDTLLSNPKHLEVIYTMCKKVLTSDAGEDAECHAAKLLEVIILQCRGRGIDQCIPLFVEAVLERLTRGVKSTELRTMCLQVAIAALYYSPSLLMHTLENIRFPHSPEPITAQFINQWMNDTEFFLGLHDRKMCIIGLSLLMELSSRPAVLEGVASQIIPSILLLFLGLKHIYASRVLNKPEQLSRVQGADEDENEEIPSDEDEVGEKSSSMQPASASSGNDNEDEDDDDDDDDYWGEEGLEGTALEEYSTPLDYDNGEDEYQFFSASLLRVQSSDVSWYQSLTQCLSDDQKKQLQEIYSLAQQRRSAGFTFLLKLCLFRNSVKLLCDDIRCKKRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LN42</accession>
    <name>A0AAR2LN42_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037391312.1">
        <property type="nucleotide sequence ID" value="XM_037535415.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391313.1">
        <property type="nucleotide sequence ID" value="XM_037535416.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391314.1">
        <property type="nucleotide sequence ID" value="XM_037535417.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391315.1">
        <property type="nucleotide sequence ID" value="XM_037535418.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391316.1">
        <property type="nucleotide sequence ID" value="XM_037535419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LN42"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063688.1">
        <property type="protein sequence ID" value="ENSPNAP00000076169.1"/>
        <property type="gene ID" value="ENSPNAG00000015276.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442974"/>
    <dbReference type="GeneTree" id="ENSGT00400000025005"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF128">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="27706" checksum="5B87F5F225BF518F" modified="2024-11-27" version="1">MGIESQGPTAPAGNLTAQQVKTFQKMEPKALGSIQIIIAVLCLCLSVSILQIYYEVHFYPDVIVVVVIVVQLLASGSILIHAGRFPSLFWVKTTLVVHLVSAAFATAMLGLLSRHLPYRQDSYHCEHCRRLEIYAVQQYCFKKCTYTVERNCKLLIDGLLATLVLFLVVELVICIVTILFGLKVLAKGGLQLLGVGQRVERPAAAPTPEIAPVQQAEVVVTEVEPEPAPAAVIEEVPTPPTEPQVEPIDSIAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LPK2</accession>
    <name>A0AAR2LPK2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LPK2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045001.1">
        <property type="protein sequence ID" value="ENSPNAP00000078503.1"/>
        <property type="gene ID" value="ENSPNAG00000031516.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007888"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000022">
        <property type="entry name" value="60S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL14" evidence="6">
        <location>
            <begin position="69"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="177" mass="20562" checksum="A7555DC0C65A8A8C" modified="2024-11-27" version="1">MREKWYARTSGSAPSFLPRSAIMVFKRFVEIGRVAFVAFGPNEGKLVTIVDVIDQNRALVDGPCTGVRRQAMPFKCMQLTDYVIKIPHSARQKFVRRAWEKAEINKKWTESSWAKKIEARQKRAKMNDFDRFKVMKAKKMRNRIIKREIFVDLQLAALLVSIFTNPPQNRRGPHSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LUP4</accession>
    <name>A0AAR2LUP4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MRPS33</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS33 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551333.1">
        <property type="nucleotide sequence ID" value="XM_017695844.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017551334.1">
        <property type="nucleotide sequence ID" value="XM_017695845.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037401955.1">
        <property type="nucleotide sequence ID" value="XM_037546058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LUP4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079452.1">
        <property type="protein sequence ID" value="ENSPNAP00000080313.1"/>
        <property type="gene ID" value="ENSPNAG00000034613.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426400"/>
    <dbReference type="CTD" id="51650"/>
    <dbReference type="GeneTree" id="ENSGT00390000011401"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="106" mass="12648" checksum="501F6CCBB0D53D96" modified="2024-11-27" version="1">MSGLSSYAVRMARLSARIFGDVVRSTDSKSMKVVQLFKEPPMAQRKEVYDWYPQHKIYYSLTQRLRYMGLFRDEHEDFKEEMRRLRKLRGKGKPKKGEGKRATKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M041</accession>
    <name>A0AAR2M041_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2M041"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065662.1">
        <property type="protein sequence ID" value="ENSPNAP00000080354.1"/>
        <property type="gene ID" value="ENSPNAG00000028573.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160511"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050254">
        <property type="term" value="F:rhodopsin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF29">
        <property type="entry name" value="RHODOPSIN KINASE GRK7-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="13">
        <location>
            <begin position="56"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="184"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="445"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="525"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="567" mass="64903" checksum="B38B572577712B71" modified="2024-11-27" version="1">MGGLENLVANTAYLNAQGGDDKELRRRRRSLLLPRPEQCAAIRSSIPLDFEQVCERQPIGKKLFRQFLLASKPEYTAAAEFLDELNEWDLEESGSKDKARQNIMNKFCRAESNSFLSYLSGDVTDKCKAVSEKDFDEVMMGKVKDATKKFLKGKPFTEFHSSLFFDRFVQWKAYEKQPISEKYFYEFRTLGKGGFGEVCAVQVKNTGHMYACKKLDKKHLKKKGGGAMSMLEKRILEKVNSRFLVNLAYAFDTTTHLCLVMSLMTGGDLKYHIYNMGERGIEPNRVIYYTAQITVGLLHLHAMDIVYRDMKPENVLLDSQGQCRLSDLGLAVELPSGKTICQKAGTSGYMAPEILKEEPYRMSVDWWALGCSIYEMVAGRLPFKDYKEKVQKEEVARRTLEDECKFEHKGFDAASKDIISLFLKKKPEERLGCNGDDLRKHEFFKSISFPRLEAGLIKPPWVPKPNVVYAKDMGDIRDFSEVKGVEFDSNDEKFFKEFSTGAVSIPWQQEMIDSGLFDELNDPNRKESSWGLKENKQSKSCKKRQQQGSQKRLSVNKQQKCTDGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M5L6</accession>
    <name>A0AAR2M5L6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM165</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M5L6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069184.1">
        <property type="protein sequence ID" value="ENSPNAP00000082301.1"/>
        <property type="gene ID" value="ENSPNAG00000035207.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005261"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="GDT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049555">
        <property type="entry name" value="GDT1-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN HTP-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="GDT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01214">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043093269" description="GDT1 family protein" evidence="6">
        <location>
            <begin position="31"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="376" mass="41129" checksum="CEC88B8E9D699584" modified="2024-11-27" version="1">MPLRAGFRHAEANLFGVLLAIILILTRVSAIQEDSKVIEEQQPVEKAATGQPAGPAVSQDETGKGNLGFIHAFVAAISVIIVSELGDKTFFIAAIMAMRYNRLTVLAGAMLALGLMTCLSVLFGYATTIIPRIYTYYISTALFAIFGIRMLREGLRMSADEGQEELEEVQAEIKKKDEELQRYKLANGATDVETGTAATMLPQRKWHSVISPIFIQALTLTFLAEWGDRSQLATIVLAAREDPFGVAVGGTLGHCLCTGLAVVGGRMVAQKISVRTGKNLLLNKMGFTSKQYKLINSWITALCLPPQLQSLEGSFSLPLLSLLSSSSQKLDSDWRKDLLVFFCKYQLSCALLTKRDCKQRLSVNIGVCMTYVCLCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M5T3</accession>
    <name>A0AAR2M5T3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translation initiation factor IF-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MTIF2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568366.1">
        <property type="nucleotide sequence ID" value="XM_017712877.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017568367.1">
        <property type="nucleotide sequence ID" value="XM_017712878.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017568368.1">
        <property type="nucleotide sequence ID" value="XM_017712879.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M5T3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045160.1">
        <property type="protein sequence ID" value="ENSPNAP00000082672.1"/>
        <property type="gene ID" value="ENSPNAG00000006293.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436416"/>
    <dbReference type="CTD" id="4528"/>
    <dbReference type="GeneTree" id="ENSGT00900000141103"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000007">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000072">
        <property type="entry name" value="translation initiation factor IF-2, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF20">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="160"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="708" mass="78709" checksum="A5E6CEF4AE2F7CD1" modified="2024-11-27" version="1">MKQMAVLIRQALSASRRDIPNMAAVCPSTAWAHTPPYAHHARYLAVKQGRGHNNSQKTSVKPLKVEKQEKQEVEIKQSMSVSELAQAMNKDIDHVYEALLNTSVDLEHLEPNTMLGEKWIKEAIKRCGMKYKWAKIVETKVRENKDIQRQPPPDLARLVNRPPVVTIMGHVDHGKTTLLDSLRKSQIAAQEAGGITQHIGAFLVQLPSGEMITFLDTPGHAAFSAMRARGALITDIIILVVAADDGVMNQTIESIQHAKKAKVPIIVAVNKCDKPQADPKKVKRDLLAYDVVCEELGGDVQAIHISALQGENLLELAEAIIALAEVLELKADPLGLTEGTVIESRTDKGKGPVTTAIIQRGILKKGCILVAGKAWAKVRLLFDVNNQAVAEAGPGTPVEIVGWKELPSVGEEILEVESEQRAREVVEWRTYTEEQNKLKEDQRVIVANQMQHQESYKKERERLAHLSWKQRKAVLYRANKHRMACRPSERAETEELSLSLIVKGDVEGSVEAILNILESYDADEQCQLNIVHFGVGDISENDINLAEAFSGTIYGFNVAANKSIQDIAAKKQIPLKMHRVIYHLIDDLKEELGSKLAPTTEEKIIGEASVLATFDVTIGKKKVPVAGCRIQKGQLDKKMKFRLTRGKEILWEGSLTALKHHKDDVQIVKTGMECGVSLDKVIEFNVGDEIICYEEIEKPQRISWDPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BJZ0</accession>
    <name>A0A3B4BJZ0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Complement component 1, s subcomponent</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">C1S</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
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        <text evidence="13">The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.</text>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548312.1">
        <property type="nucleotide sequence ID" value="XM_017692823.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000000320"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014050.2">
        <property type="protein sequence ID" value="ENSPNAP00000000320.1"/>
        <property type="gene ID" value="ENSPNAG00000007432.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424659"/>
    <dbReference type="CTD" id="2199"/>
    <dbReference type="GeneTree" id="ENSGT00940000156047"/>
    <dbReference type="OMA" id="YTAGHTF"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000341">
        <property type="entry name" value="Fibulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000078">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000139">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000020">
        <property type="entry name" value="Anaphylatoxin/fibulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050751">
        <property type="entry name" value="ECM_structural_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056612">
        <property type="entry name" value="FIBL-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055088">
        <property type="entry name" value="Fibulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034:SF158">
        <property type="entry name" value="FIBULIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24532">
        <property type="entry name" value="FIBL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00104">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01177">
        <property type="entry name" value="ANAPHYLATOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01178">
        <property type="entry name" value="ANAPHYLATOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017376794" description="Fibulin 2" evidence="13">
        <location>
            <begin position="28"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphylatoxin-like" evidence="14">
        <location>
            <begin position="616"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphylatoxin-like" evidence="14">
        <location>
            <begin position="692"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="778"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1079"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1122"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1164"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="1203"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="525"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="824"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1405" mass="156469" checksum="58BFAF92F0FEFF1D" modified="2018-12-05" version="1">MQTTGSPLVRMGLLKLMLLICVSGGLCQRDCTGVDCPLLQNCIEEVLEVDACCASCVQTGCTCEGYQYYDCVNAGFQKGRVPEGESYFVDFGSTECSCPMGGGRISCHFIPCPDLPANCIEMSEPADGCIQCERIGCVYKEQKYHAGHSFHMDPCQVCHCPNDGGKLMCYPIPDCDPRKVHKPMLATTTEENPLGRLHKDPIRHIFNNHQGSRGSISKPFPVSHSDNLPAFKVNPHKSHMNEEEEEEDYDYPTTDSLEPSMHDLVSPAGSSIISDSYPENVTAHQVFHTGTKQELRERFGFHEATTDRPWFPLHKERTDEGKYILHKGNPKKDTLSITKDETRGAIFNIPDDSASKEKLPVFREMTYEEQFSIDRDTSERQSFSLYKDNANEEVHEKSITDSEGLAAPTGTTDAEMFDLYGDTTHSEGVTDSPVIVDPTTHELPKMETTTTWQTTRNTVSSLQNQSLTTEAVEHYGTLIEEDITQEERNERTVMLSSVTDIHRNHGPGGMGGAQEENTVSNLDDKEVTSMSPVTPEKASKEHGTKPESYTIPFIRFSPTSQPALRVKADERQPPTKQSRSLLEEAGEEEREERENTLSFLPKAHEEPSDPALLERCCESGQNWASEHQHCNHLPTLTHHNSVCGALQEQCCAGTLRESRCLTGMNAAKAGEVCEESNSPLCGEDTKKECCSCCALGLRLRQEGKGCDVHQHLSYPCGHIFLTCCEEEEEGLSTPVLRRKEKPKPTLLPKRVAERQFPKQPFSVEDSEHAANSVEKLEDVDECQRYEGQHCHHTCINTPGSYKCACRTGYLLLQDGHTCALENPEEENRVKEEDSPFTLATPPQETTPQITPLHNLCAGKGPCSQQCSVVRGKAQCSCFPGFSLKADGQNCEDVDECRKATHTCSLEKVCVNTEGSYQCVSLNERCDVGFILNYNRECVDFNECVTNTHTCLSNERCVNTVGAFVCERQISCSSGYQLRNGVCEDIDECVLRSHNCAPGLQCQNTLGSFHCSPKQQRCLTGFTQDPHGNCIDVDECSVLSEPCTLGFNCINTVGSYTCQRKIMTCSRGYHSSPDGARCVDVDECVMGTHRCGEGQVCHNTPGSFRCDCQTGYQYDSSRRTCVDVNECWRFPGRLCAQSCENTPGSYQCSCTTGFSLAFDGKNCEDLNECDSNPCSQECANIYGSYQCYCRLGYYLKEDGHTCEDIDECSQSIGNLCAFQCVNVPGSYQCACPPEGYSMSPNGRTCRDIDECATGVHNCSATQTCYNIQGSFRCLSFTCPENYRKVSDTRCERTSCPSFQNCQNMPLRITYYQLSFQTNIVIPAQIFRIGPSPVYSGDNIIISIPRGNEEGYFSTRRLNSFTGAVYLQRQIHGPRDFFIDVEMKLLRQGTFTTFLARIYVFITAHSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQI4</accession>
    <name>A0A3B4BQI4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQI4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000786"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013266.2">
        <property type="protein sequence ID" value="ENSPNAP00000000786.2"/>
        <property type="gene ID" value="ENSPNAG00000000426.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287001"/>
    <dbReference type="OMA" id="RMFYHNY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031686">
        <property type="term" value="F:A1 adenosine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001621">
        <property type="term" value="F:G protein-coupled ADP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045031">
        <property type="term" value="F:G protein-coupled ATP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14982">
        <property type="entry name" value="7tmA_purinoceptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF2">
        <property type="entry name" value="P2Y PURINOCEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="31"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="308"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="431" mass="48938" checksum="2F18F73CD25F69A0" modified="2024-11-27" version="2">MTSPGLGVMRIVQLFLMPGFFLGVMVLGLPLNLFSLWVFSRRLSQSNRSVLFLHNLALADTFWLLALPFLIQYHLAGMQWSLGQAFCKAIRLLYHNYFYLSIFFVTCLSVDRYLAIVHPLRSPVLLTHRRSVMLCISVWLLAIVISLPVAQLSTTAHCQQGNRTVCIIYVFMGDIEESLLYSVCCSFLGFFFPFTALSYCYICSVCQLRRRADPRLRGLACELGAAMVLFGLFYLPYHLSRNAALVVMALMPQDSSAREVADLTFSLEMCVCSLTSCTNPLFSCFAGRPIRRELRAALTRLWKRHRVEPETRRKKQERNLSEARRPVRQELKSKSHSMPPKHHADSDVTPTHSDVTPTPLKRSLSSALMLAYFLLSCLRGPWRPSLSWFPCHRLSSAGLTLTFKIMKSKHTHVPMAESGDCGCDGDQPYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BR04</accession>
    <name>A0A3B4BR04_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fibrillar collagen NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">COL11A1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BR04"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001010"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012885.2">
        <property type="protein sequence ID" value="ENSPNAP00000001010.2"/>
        <property type="gene ID" value="ENSPNAG00000000539.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154535"/>
    <dbReference type="OMA" id="KGNMQGP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060536">
        <property type="term" value="P:cartilage morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030903">
        <property type="term" value="P:notochord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000002">
        <property type="entry name" value="Collagen XI alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000016">
        <property type="entry name" value="Collagen XI alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000885">
        <property type="entry name" value="Fib_collagen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1082">
        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01410">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00038">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51461">
        <property type="entry name" value="NC1_FIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibrillar collagen NC1" evidence="9">
        <location>
            <begin position="887"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="411"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="583"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="669"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="785"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="447"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="785"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="823"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001010.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1117" mass="117955" checksum="88F4484B55F4A004" modified="2024-11-27" version="2">VTATDLRWNNFTISAFSLLTAETVDVLKVLEFHNSPEGVQKTSGFCMNRRASQPDTAYRVGKAAQISAPTRQLFPNGVFPEDFSIMTTIKPKAGLQSFLLSIYSERGVQQLGVEVGRSPVFLYEDQTGKPAPEDYPLFRTLNLADGKWHRVAISVEKKTVTIIVDCKKKITKPLGRSDQAALDTKGITVFGSRILDEEVYEGDIQQLLIVSDPKAAFDYCEHYSPDCDTPHKDMLQAQEPEEEYTTAVDYELYDYYDGPATTDTTSEFSETQLNNLDGEYYTDSELDYGTVDATQIQPSVPTTGPNEAVEEEIVDHFVPEPAQTGLDPTAGPEPTQPVATGADAYDFKEYDLKEYDVNELDKSQYEFGAYDEFGNAAPNPTATYEDEFGLGVPAETDVEETQVGTDVSIGMLIEGPPGAPGPAGLPGPPGLQGPPGPPGDSGDRGPAGRAGLPGADGMPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARVSAAGGKTAQEHFTAGPGLGGVTGGDRGFDGLPGLPGEKGHRVSPASLLSCLTPSFFSSFCFQGQLGPQGPIGYPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGVLGPRGEDGPEGPKGRAGPPGESGPMGLAGEKVRISFCLLLSRFIIGFIKRLTLTDGEPGPRGQQGMFGQKGDEGSRGFPGPPGPIGLQVSPSQPRGEPGAEGPPGKTGPVGPQGPPGKPGPEGLRGIPGPVGEQGLPGASGQDGPPGPMVREEGFHFLDVHVSVSQGHPGLIGLIGPPGEQGEKGDRGLPGPQGTPGPKGDGGPQGPKGAKGSSGPAGQKGDTGVVGPPGPPGPPGEIVQPLPIQSPKKTRRSSDMQADEATIMDYGEGMEDIFSSLNNLKQDIERMKYPMGTQNNPARTCKDLQLCHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIHPDRKSSGVSIMSSWYKEVPGSWYSSFTHGQLLSYVDAEGNSINPVQMTFLRLLTASARQNFTYSCHQSVGWQDATTDSYHKALRFLGSNDEEMSYDNSPYIKALVDSCATRKGHGKTVIEINTPKIDQVPVVDVMLSDFGDPNQKFGFEVGPACFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BR92</accession>
    <name>A0A3B4BR92_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SEC16B</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. Involved in peroxisome biogenesis. Regulates the transport of peroxisomal biogenesis factors PEX3 and PEX16 from the ER to peroxisomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">SEC16A and SEC16B are each present in multiple copies in a heteromeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SEC16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570446.2">
        <property type="nucleotide sequence ID" value="XM_017714957.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BR92"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002232"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010791.2">
        <property type="protein sequence ID" value="ENSPNAP00000002232.2"/>
        <property type="gene ID" value="ENSPNAG00000008668.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437689"/>
    <dbReference type="GeneTree" id="ENSGT00940000160138"/>
    <dbReference type="OMA" id="YAPVPMY"/>
    <dbReference type="OrthoDB" id="8918678at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09233">
        <property type="entry name" value="ACE1-Sec16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024340">
        <property type="entry name" value="Sec16_CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF11">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC16B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12932">
        <property type="entry name" value="Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="15">
        <location>
            <begin position="256"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="14">
        <location>
            <begin position="429"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="184"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="749"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="842"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="753"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="846"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="903"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="923"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="963"/>
            <end position="973"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002232.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="973" mass="107659" checksum="9FC88E7B12797890" modified="2024-11-27" version="2">MESRGHGWYGPGPQYHPSAQPPRHRDRDDYYRPQHPGQGHYPSPDPRERERERQWYSPDPRYRQYLTSPPPRPEFMPGYSSASQSNQSYGPNRARPLSRQAYDYPPQSYWDYRDHYGYYDHGYYRGQYGYPEAGGWPAQDGWRSEVYQGRGAEQEWTGATYPDQQGYGHRRHSVPYDGGVSGMERRKSMENGNGAGETCEPGALASSKTSGLSSSSYELSQYMNGVEQSDAAPPPLSEPEVETKAFAPLKFCVPHVAVSFGPAGQLVRVSPALPSHGEPAQVELHSLEVILSDTREQHDMRDFPGPLAREDLHKVDVINFALQMADACLKDSALSDASTAALLWHLLVLLCRQNGRIVGSDIAELLVRGSQSSRGVCEGDSERSDIGSLIDLSETPSPEMANLNNSDLLTGNPLTEEQTSEQNLQNYTKLLLAGRKKEALESAMRSGLWGHALFLASKMDGRAYTTVLSRFTGSLASSDPLQTLFHLLSGRIPTVATCYSTEKWGDWRPHLAVMLSNETEDSDMHCKSIVCMGDLLASRGLLHAAHICYLTAHSPFGWYTNKAQRLVLLGSSHGVSFPKFCQNSVIRCTEVFEYCQRLGEPSFTIPSFQVYKFLYACRLLDCGLASQAFHYCEVVAKSLLRIQEPHIILLGEVIKLVDRLKHSEAQFSETGADLTWLDLLRRRLQDIQMGSSGHSGAYVSAAEDSAWTHEESREHADFNPDDLSNSEEMIQCFHPDTSHSGAVQEHCEHHESAAQQPHSFVPSEEQSYPSYTSEECSYPPEAQPYAPEEQLPQTYAPVPMYSWQNTLAAPPSQPLGMPGLPPVPAGQDGANQSSYHHTAAGLIPRSHSNTGVEFSTDTNMTPENGTRFGGEDLETEANTKETTSQKSEKSSKSGWFSGWFRSKPRESPQEEITQDKPAHMEPAPAPASPPLMPPIPMASPILASQPQAAGINPFSRRAGQKPGLTSGSMTQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSU0</accession>
    <name>A0A3B4BSU0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferlin family.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000001586"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011904.2">
        <property type="protein sequence ID" value="ENSPNAP00000001586.2"/>
        <property type="gene ID" value="ENSPNAG00000005929.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154741"/>
    <dbReference type="OMA" id="KHCGRTQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08373">
        <property type="entry name" value="C2A_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04011">
        <property type="entry name" value="C2B_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04018">
        <property type="entry name" value="C2C_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04017">
        <property type="entry name" value="C2D_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04037">
        <property type="entry name" value="C2E_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08374">
        <property type="entry name" value="C2F_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000009">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000026">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037726">
        <property type="entry name" value="C2A_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037720">
        <property type="entry name" value="C2B_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037722">
        <property type="entry name" value="C2C_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037723">
        <property type="entry name" value="C2D_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037724">
        <property type="entry name" value="C2E_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037725">
        <property type="entry name" value="C2F_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012968">
        <property type="entry name" value="FerIin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012560">
        <property type="entry name" value="Ferlin_A-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012561">
        <property type="entry name" value="Ferlin_B-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032362">
        <property type="entry name" value="Ferlin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055072">
        <property type="entry name" value="Ferlin_DSRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF55">
        <property type="entry name" value="MYOFERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22901">
        <property type="entry name" value="dsrm_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08165">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08150">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08151">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16165">
        <property type="entry name" value="Ferlin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01200">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01201">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01202">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2023"/>
            <end position="2045"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="190"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="358"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1128"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1292"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1541"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1770"/>
            <end position="1920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="128"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="960"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1974"/>
            <end position="1996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1974"/>
            <end position="1987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001586.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2057" mass="232902" checksum="DC826E2622A7E945" modified="2024-11-27" version="2">MLKVVVESAKGIPKKTLGNPDPIAGVIFRGEKKKTKAISNELNPVWNEVIEFDLKGSSLDSSSFIEVVVKDYETIGKDKLIGSTKISLRDLASGQARSLPSRNVPLINEKKQDIGATINLVIGYEPPASAPPNPNAQIDGDVPAGESAVSPAGKDGTDGDEDPGEWGDTRASGEPPTPGQPGKPPQKMMRMGRKRQKALANKPQDFQIRIRIIEGRQLPGNNIKPVVKVSVCGQTHRTRIRRGNNPFFDEIFFYNVNMLPSELLDENIYIRTAETFSHYLSHDNTTVRTVFLMYLNVLYHLYIIYSVMCEAHAIMRKWLLLSDPDDSSSGARGYLKVSMIIMGTGDDPPVEKREQIEEVDDIESNLLLPAGVTLRSATLSLKVYRAEDMPQMDDAFAQSVKHMFGGESDKKNLVDPFLEVSFAGKKLCTKIIEKNANPEWNQLVNMQVKFPSMCERIKLTMFDWDRFSSNDAVGTTYLDLTKIASSGGEIEAETFENGTLPAAEVERVGTEVGFLPAFGPCYLNLYGSLREFSDLSDPYEELNLGKGEGVAYRGRVLIELSTRLDDKIDKKVDDLSNDDILVAQKYQRKRKYSLCAVFHSACMLQEPGEPIQFEVSIGNYGNKMDSTCKPLASTTQYSCAVFDGNNYYYLPWANTKPVVILTSFWEDISHRLDAVNIVLFTADRLKANVTSLKTAIFAKVPETRLAEIWLKLINQLIEDLSSFQLPDIEGKANLTALDIQIKKLRDRAIVSIREAARHMREEATDVKATLTDIEEWLERLQQIAEEPQNSMPDIIIWMLRGEKRMAYARIPANQVLFSTHSDQATGKYCGKTQTIFMQYPMDKNKGVKIPVQLRLNVWLGLSAHEKKFNTFAEGTFSVYAEMYENQAQMFGKWGTTGLVGRHKFSDVTGKIKLKQECFLPPRGWEWEGDWFIDPERCLLTEADAGHTEFTDEVFQNESRFPGGEWKPATEPYTDVNGEKAQSPEEIECPPGWKWEDEWSFDSNRAVDEKGWEYGITIPPDDKPSSWVAAEKMYHIHRRKRLVRLRKKTKNTPTVEKKEAGDGWEYSSLIGWKFHRKERSSDTFRRRRWRRKMAPGDSVGASAIFKLEGALGVDIDEKTSKKDAAKLFGANTPTVSCSFNKSFMYHLRVYVYQGRNLLPMDKDSFSDPYCHVSFLHVSKTTEIIKATLNPTWDQTLLFNDIEIYGDPQTIAHNPPSVVMELFDSDQVGKDEAMGRCVCPPVVKLNPGSPVTPKLLWFPVTMKGRSAGEILVAAELLLKDKTNESEMPLVPPKRAEKLYMVPQGIRPVVQLTAIEILAWGLRNMKTYQMAAVSSPSLIIECGGHTVQTAVIKNIKKCPNFPGSVLFLKVLLPKEEMYTPPIVLKVIDHRPFGRKPIVGQCTVDSLEEFRCDPYMDIAEVSRTARVAMMSAAPGDVIIDIEDRRPVLDSQVQIVDEAVDWWSKFFASVGEHEKCGPYLQKGYATLSVYDKELEAMPDFHGLTDFCNTFKLFRGKTDDDEEDPSVVGEFKGSFRVYPLSDDPEVPAPPRQFRELPESGPQECVVRIYVVRCIDLQPKDNNGLVRIYSCELRKVYCMINKTSGFVGSVLMFEMSCFLPQDKDLKISIYDFDLLSRDEKVGDTIIDLENRLLSRFGSHCGLPQMYCVSGVNQWRDQLKPSQILQNLARLRGLPPPQIADDGSSLSFGGREYNLHDLEASKVLHQHLGPAKERICLHVLRKQGLVPEHVETRTLHSSYQPTLSQGRIQMWVDVFPKNLGIPGPPVDITPRKAKKYFLRAVIWNTSDVILDETSITGEHMSDIYVKGWMPGMEEDKQKTDVHYRSLDGDGNFNWRFVFGFDYLPAEQLCLVSRKEHFWSLDKTEFRIPPKLIIQIWDNDKFSLDDYLGTVELDLIHLMPPSKTPEKCSLNMLTQGAGRNLSSNSLFAQKSVRGWWPCAMEEDGKKLLTGKVEMTLEIVDEKEVEERPAGKGREEPNMNPRLEPPNRPDTSFLWFTNPCKTMKFIVWRRFKWVFIGGIILILVLLFVGVLLYSLPNYISMKIVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BTT8</accession>
    <name>A0A3B4BTT8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">KASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SYNE3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus outer membrane</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nesprin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566769.2">
        <property type="nucleotide sequence ID" value="XM_017711280.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BTT8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001961"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011236.2">
        <property type="protein sequence ID" value="ENSPNAP00000001961.2"/>
        <property type="gene ID" value="ENSPNAG00000001037.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435438"/>
    <dbReference type="GeneTree" id="ENSGT00440000039367"/>
    <dbReference type="OMA" id="EISACHD"/>
    <dbReference type="OrthoDB" id="9838382at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045111">
        <property type="term" value="C:intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012315">
        <property type="entry name" value="KASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052403">
        <property type="entry name" value="LINC-complex_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057932">
        <property type="entry name" value="Spectrin_SYNE1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057933">
        <property type="entry name" value="SYNE3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535">
        <property type="entry name" value="MUSCLE-SPECIFIC PROTEIN 300 KDA, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535:SF2">
        <property type="entry name" value="NESPRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10541">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25803">
        <property type="entry name" value="Spectrin_SYNE1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25804">
        <property type="entry name" value="SYNE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01249">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51049">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1103"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="8">
        <location>
            <begin position="1124"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="domain" description="KASH" evidence="11">
        <location>
            <begin position="1094"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="784"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="876"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="929"/>
            <end position="962"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001961.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1152" mass="131914" checksum="3A316587D1587C5D" modified="2024-11-27" version="2">MTQQEQNEFKESCEDALSWMQAVQERLRLNDNTQGPRAALEARLRETEKIRESEQDGRLKMDLVLAAADTLLNSGDEEMKNQTHSKLKDLKALWEETSTYIIHCHSRIEWVWLHWSEYLKAHEEFNMWLVKMQRTLEPQLELQLGPREKLWQLDHHRVLCSDVKAQAQLLERLVDEAAALHNRTQDTSVDENAQDALQEAYNSIRTKAEERLALVQKVAEEHQQYQNHVLEFQKWLVLKTEEVSRFTELEDTSENRLQALQELDASVAHEEKTLRYIEGLAEAVKVNTSPAGAEYITEEVEELRLAWQRLRQALVDIQEELQSSLDSHSQYLARREGLVADIGQLRALVQKMSRELESKDGERTEEHMVTQWRKYTGVRNALLAEEPRVEQLKTQLKELFRFPQDHRELSDDVLAAVKEYQSVKGRSSRLSSESETALRQVLQDPLHGFSQWSQAVSQVLEASAEVTEFSHIALLVQNIEKLLKHSVQLQERLSLLQVKADLLTAVFGKEKADSLLEELTADMRKRELLHSQLMQRKSRLQGLISRTKDFGDAYDSIQKRLASIKERFLAAEGLQPDILAKKSQADQLKVIKRDLEDSEAHITALETLVSSSPTNRTKFERLYADWKLLYKAVRVKVNESEESISEHESFHESLLNVEKWLMIMRQKLESFRGPNGEWSVENRQHEAERALGEFPEKELQLHRTDGHGQAVLAKTSEEGKVHIQRDLKRLRESWMSLHSLSLNLYRLLNGHRASGEPDSSFYAHETLNEADDFLELDQRRVPLEEAGSLRGSGSTSRARADGHPGQELDWPYEGSGLGRGSGFEADGRFVTGHDGGGQIADGQFSPGGKVKVRPEMRGKTAVDDVDSMVIGRGYEEQKDRGDEYGAGLVRRGGSRSGGRTREWVEDHAPGIEDLDLEGKRGLSKDQYLSEDQTGAGLTRRIRTSDAEFRKRQPSPYKPGAAHGFLESDVRERDHDWEALRREFEAWLRKENDKLSAVVNNQRVSSAKELKIRQNTLKGLRAGVTRGQALFQQLMDAWHSDAGREDVSMEELRYKWMLYKSKLKDAEHVKARLKPKGDGVQQEELIRTSKEKSCCGFLYRVCRVALPLQLLLLALLLLAFLLPMMDEGTSCSLSNNFARSFNIMLRYHGPPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUK1</accession>
    <name>A0A3B4BUK1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatidylinositol-glycan biosynthesis class W protein</fullName>
            <ecNumber evidence="5">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PIGW</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIGW family.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017570364.1">
        <property type="nucleotide sequence ID" value="XM_017714875.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570366.1">
        <property type="nucleotide sequence ID" value="XM_017714877.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUK1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003317"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008928.2">
        <property type="protein sequence ID" value="ENSPNAP00000003317.1"/>
        <property type="gene ID" value="ENSPNAG00000009728.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437648"/>
    <dbReference type="CTD" id="284098"/>
    <dbReference type="GeneTree" id="ENSGT00390000013520"/>
    <dbReference type="OMA" id="GLYVMQP"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="437"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="492" mass="54701" checksum="9A0092BCC29F7AAB" modified="2018-12-05" version="1">MAEAEVKVAFVSNLNGTTVAEVSLGSFLAPLCLLSRGLFLIVFHFCRGVLPLAKGAHLLLDFTMLIMPLVLSCTVLSGILHWVIIGLAVADLFVLFLLYRNKKTSSHGSILSSLDSFVHSHVESNFVPFVTAFRVLVNVKTAISILAVDFSVFPRRYAKTETYGTGVMDFGVGAYVMANALVCPEARGKKIQGSKFSHLVKQLLSVWPLILLGLVRLASVKSTGYHEHVTEYGLHWNFFFTLAIVRVVASALLVVFPVNSSWLMALVISGVYQVVLETTDLKSFITQNGGRNNFVEANREGIFSVVGYIAIYMAGVQAGLYLMQTRTLVKDWIRVVRNLLLGSFGLWLILHMCQTYIEPVSRRMANLSFCMWTVAQSLFFLSCLSIGDIVLLFAKVVSNFSHVSVSWNPSGSSQTEGKLRNKMDMLCLIQSVNRNQLLFFLLANLMTGLTNILVDTLKSNNFFAVCVLLLYMFVNSLAIFILHIKNITIKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0G2</accession>
    <name>A0A3B4C0G2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH2 domain-containing adapter protein E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SHE</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037392990.1">
        <property type="nucleotide sequence ID" value="XM_037537093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0G2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004144"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007522.2">
        <property type="protein sequence ID" value="ENSPNAP00000004144.2"/>
        <property type="gene ID" value="ENSPNAG00000010592.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443676"/>
    <dbReference type="GeneTree" id="ENSGT00940000159107"/>
    <dbReference type="OMA" id="PELVYHY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10391">
        <property type="entry name" value="SH2_SHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000067">
        <property type="entry name" value="Src homology 2 domain-containing E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051846">
        <property type="entry name" value="SH2_domain_adapters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035042">
        <property type="entry name" value="SHE_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127">
        <property type="entry name" value="HEAVYWEIGHT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127:SF29">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING ADAPTER PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="346"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004144.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="445" mass="49006" checksum="BF1329B96D00CAC8" modified="2024-11-27" version="2">MAKWFKDFPTSLKTGSDRIRSASESGPQTRPKPGLTVSAGAKGSLRKNSENGGGGGGVGSLLSGRTRKNSAIELGRNHWSSGSGSVKDGKIWEILIPGKNKKNPKMEGFEDHRTLRTTSIADAYMSRMIKVDKQDKCAKYGSGPAEGSGMDEKGKSPVKTETLIILEDYADPYDAEKTREQREAEREGENDGYMEPYDAQLIITEIRRRGSKDLLKVCVVLNGGEGEGSGPPQIYDVPYEEVLEGIPLTRPESDPRPPAEYELPWEWKKEQIVRALSAQFDGMERACKEDGSTPAVKQQTLRSVKSWTPKSLRPSPPSPTSPSPNTSPDTESPKVDPTLPLEKQSWYHGSVSRQEAEAQLQNCREASFLVRDSESATSKYSIALKTSQGCVHIIVAQTKECGYTLDQSSCVFPSIPEVVHHYCTQRLPFTGAEHMTLQHPVPRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2C8</accession>
    <name>A0A3B4C2C8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase CHFR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 18" type="primary">RNF8</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CHFR family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the RNF8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551114.1">
        <property type="nucleotide sequence ID" value="XM_017695625.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2C8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006057"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004220.2">
        <property type="protein sequence ID" value="ENSPNAP00000006057.2"/>
        <property type="gene ID" value="ENSPNAG00000012148.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426266"/>
    <dbReference type="CTD" id="9025"/>
    <dbReference type="GeneTree" id="ENSGT00400000022349"/>
    <dbReference type="OMA" id="RRKDECP"/>
    <dbReference type="OrthoDB" id="341587at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22663">
        <property type="entry name" value="FHA_RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16535">
        <property type="entry name" value="RING-HC_RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000242">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03067">
        <property type="entry name" value="RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017335">
        <property type="entry name" value="RNF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FHA" evidence="16">
        <location>
            <begin position="42"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="386"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="174"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="230"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006057.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="482" mass="54655" checksum="878F8499FB8C7565" modified="2024-11-27" version="2">MEEVDEEQSSAEGENCVNDKTWCLKRVGKDTGWLRLYENSEVTIGRGLNVTHQILSVSCPLMISRNHCMLKQREDGQWTVTDNESINGVWVNGTRISPRKPHAIQQGDVVRLGVPLDGNPVEFEYILVRRRLSKIKDFLTKGVAAEAVANISVPKPKKSKRKFGTDEVEAGTTLDSKSKLYRSSNTDKSSAQPCPTAAAHQSVLPAYSGPNDPPGSSAHCSDGSQQLATLQRYNNNLRALKERMEVTQKRAAELAGQERASAEQQRKMQELQEQMEVLRGQLNSQQEMALHRVEMLEKSICEEERRLEMQQAQQNEVGLKKQLEEALKEQRKVIEELKHAREGFKEVLQAKDKELEVTKEEKEKARAQKEEVVTQMTEVLENELQCIICSELFIEAVTLNCAHSFCQHCIGDWRRRKDECPICRQAILSQTRSLVLDNCIDRMVENLSTEMKERRLALIRERKGERSEQSPICGSGSDPGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2N6</accession>
    <name>A0A3B4C2N6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bestrophin homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BEST4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Forms chloride channels.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C2N6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005270"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005572.2">
        <property type="protein sequence ID" value="ENSPNAP00000005270.2"/>
        <property type="gene ID" value="ENSPNAG00000011559.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160852"/>
    <dbReference type="OMA" id="FIDNIAC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000615">
        <property type="entry name" value="Bestrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021134">
        <property type="entry name" value="Bestrophin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736">
        <property type="entry name" value="BESTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10736:SF55">
        <property type="entry name" value="BESTROPHIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01062">
        <property type="entry name" value="Bestrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005270.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="456" mass="53265" checksum="0BE5EC5A37A3C56B" modified="2024-11-27" version="2">MTVSYTLEVANARFAGFTKLLFRWKGSIYKLLYKEFLVFCFLYSFFSVIYRCVLSGGQQELFERAARHCNKFAKLIPMSFVLGFYITQAFQRWWGQYTSFPLPDNLMMVVSGNVHGADERGRLLRRTMMRYANLSSVLILRSISTRVHKRFPTMQHIVEAGFMTEEELKTLDHLYSDFNKYWMPLTWFANLASKAREEGRVKDDMALKLLMDELNNYRAKCSMLFHYDWISIPMVYTQVVTLAVYSFFAFCLIGRQFVKPERADEHKVHVDLYVPVFTLLEFFFYAGWLKVGELIINPFGEDDDDFETNQLIDRNIQVSMLAVDDMHQSLPPLEKDKYWTEKSFSLPFGPAKPVFMGSTYDMSYQIPMLPVSVWPPTPKKQKKSQRLSCCTKKVKSKKKTHTKDMETVDIMPAKPCGCEQQQQQHYEESVERPQCVVTLGEAEKEQETDYTNKSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3F1</accession>
    <name>A0A3B4C3F1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ELP5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037400377.1">
        <property type="nucleotide sequence ID" value="XM_037544480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3F1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005555"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005109.2">
        <property type="protein sequence ID" value="ENSPNAP00000005555.1"/>
        <property type="gene ID" value="ENSPNAG00000011749.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413726"/>
    <dbReference type="GeneTree" id="ENSGT00390000009210"/>
    <dbReference type="OrthoDB" id="166907at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19496">
        <property type="entry name" value="Elp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="262"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="295" mass="33358" checksum="E8CD94DFE5A5D754" modified="2018-12-05" version="1">MLLEVSEAAQAGGVVIIRDTTSYSGRHLLKYYINCALKREEDVHVLGFEVPEQELRAGLDSNYMHWFHFHNAYIDPLGWTKQSSFTVKQFTTADVTRLVREIQHAKASILVIDSLSWVIRHHNPVAVCQQLQELKRGGSVRMIFGLLHTDLHQQGIVDSICHLASTVISVTSVNHAHHAVAKTMHRKKSGKVIREEEFFTVKDDLTLSIEKRSGQSGHVQMQPDTKADPTSNLTFNLRLSEVEREAREKVALPFVFSQEKKSALLKQSQGSGRIIYEPDASDDFDEEDPDDDLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3G6</accession>
    <name>A0A3B4C3G6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cold shock domain-containing protein E1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CSDE1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of a multi subunit autoregulatory ribonucleoprotein complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1. Interacts with STRAP. Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, HNRPD and SYNCRIP. The interaction with PABPC1 is direct and RNA-independent. Interacts with EIF4ENIF1/4E-T.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the UNR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570246.1">
        <property type="nucleotide sequence ID" value="XM_017714757.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3G6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005570"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005075.2">
        <property type="protein sequence ID" value="ENSPNAP00000005570.2"/>
        <property type="gene ID" value="ENSPNAG00000001958.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437583"/>
    <dbReference type="CTD" id="7812"/>
    <dbReference type="GeneTree" id="ENSGT00390000016950"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905172">
        <property type="term" value="F:RISC complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000055">
        <property type="entry name" value="Cold shock domain containing E1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000088">
        <property type="entry name" value="cold shock domain-containing protein E1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000093">
        <property type="entry name" value="cold shock domain-containing protein E1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000094">
        <property type="entry name" value="cold shock domain-containing protein E1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056400">
        <property type="entry name" value="CSDE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024642">
        <property type="entry name" value="SUZ-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12913:SF2">
        <property type="entry name" value="COLD SHOCK DOMAIN-CONTAINING PROTEIN E1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12913">
        <property type="entry name" value="UNR PROTEIN N-RAS UPSTREAM GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23456">
        <property type="entry name" value="CSDE1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12901">
        <property type="entry name" value="SUZ-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51938">
        <property type="entry name" value="SUZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CSD" evidence="15">
        <location>
            <begin position="78"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="15">
        <location>
            <begin position="257"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="15">
        <location>
            <begin position="421"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="15">
        <location>
            <begin position="593"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="CSD" evidence="15">
        <location>
            <begin position="748"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ-C" evidence="16">
        <location>
            <begin position="823"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005570.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="873" mass="96665" checksum="B961DBF369916745" modified="2024-11-27" version="2">MEKLCADAPAPSVVTTQAPAPSTFCQTIPRSASVSCHAPRAATGKRHKRTPLYQRSMSFDPSLLHSNGFANGTGVGLRETGVVEKLLTSYGFIQCSERQARLFFHCSQYNGNLQELKIGDDVEFEVSSDRRTGKPIAVKLVKIKAEVLPEERISGQVGPDLHASPFTMLHGYIHPHPVVSAIPNHLDGKSAPGQVPTGSVCYERNGEVFYLTYTPDDVEGNMHLDTGDKVSFYMETNKHTGAVSARNIVLVKKKQMRCQGVVCATKEAFGFIERADVVKEIFFHYSEFKGDLEALQAGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEQFEGTVTKVIPKVPTKNQNDPLPGRICAKINATEKELLFGEKDTKSKVTLLEGDHVQFNISTDRRDKLERATNIDILPDTFHFTKETREMGVIAAMRDGFGFIKCVDRDARMFFHFSEVLEESPLHISDEVEFTVVPDMLSAQRNHAVRIKKLPKGTVSFHTQSEQRFIGVVEKETTGASNTKTNASPSKGKEKDAEEGVIAYEDCGVKLTVPYHIKDLDGCVFPQLGDKVEFAISEVKRTGQQSAVSIKVLNRTTNTKRLLGFIATLKDNFGFIETANHDQEIFFHYSEVCGNVDNLELGDTVEYTQAKGKGNKVSAEKVTKVPPVNGVGEDVSSNVSLGKVIRPLRSVDPSQTEYQGLIEVLEEGSSKGQNYPFGIVGMTNKGDCLQKGELVKFQLCTIPQTGQRMACNVVPQRRALVECVKDQFGFITYEVGESKKLFFHMKEVQDGLELQAGDEVEFSVILNQRTGKCSACNVRRVSECPKPVATPRPDRLVNRLKSITLDDASAPRLVIIRQPRGPDNSKGFNVERKLCKVSVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4K8</accession>
    <name>A0A3B4C4K8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PHD finger protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PHF10</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAYP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546179.1">
        <property type="nucleotide sequence ID" value="XM_017690690.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4K8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005980"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004366.2">
        <property type="protein sequence ID" value="ENSPNAP00000005980.1"/>
        <property type="gene ID" value="ENSPNAG00000012074.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423349"/>
    <dbReference type="CTD" id="55274"/>
    <dbReference type="GeneTree" id="ENSGT00940000155172"/>
    <dbReference type="OMA" id="EKHREYA"/>
    <dbReference type="OrthoDB" id="1903104at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:npBAF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="PHD1_PHF10"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WH_NTD_PHF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000202">
        <property type="entry name" value="PHD finger protein 10 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHF10_PHD_finger_1"/>
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    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
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    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888">
        <property type="entry name" value="HL01030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888:SF4">
        <property type="entry name" value="PHD FINGER PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="370"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="426"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="284"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="491" mass="55363" checksum="501CC0B2635381AB" modified="2018-12-05" version="1">MATVLSPRQCDSSPATPGTQSLKDDIEENSSDGSQAPKRRRMGSGDSSRSCDTTSHELGPTYFPAENLTEYKWPPDDTGEYYMLQEQVSEYLGVTSFKRKYPDLERRDLSHKEKLYLREQNVITETQCTLGLTALRSDEVIDLMIKEYPVKHAEYSVILQERERQRITKEYSVSTLSAHMQQQNPQRVEASKVPEYIKKAAKKAAEFNSNFNRERMEERRAYFDLQTHIIQVPQGKYKVLPPERTKTGPYPVALIPGQFQEYYKRYSPNELRYLPLNTALYEPPLDPELPALDSDGDSDDAEEGKGEDSKKNKGSSDSSSGNTSDGDSQDSGVQSKGKNKDRAGTQSKDATPRPLQHKSVPGYKPKVIPNAICGICQKGKESNKKGKPEALIHCSQCHNSGHPSCLDMSAELVVLIKTYPWQCMECKTCTVCEQPHHEEEMMFCDKCDRGFHTFCVGMDSIPLGCWVCDCCNNDSSSPKKKWTAKTPKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4T2</accession>
    <name>A0A3B4C4T2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RAMP2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4T2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005032.2">
        <property type="protein sequence ID" value="ENSPNAP00000005589.2"/>
        <property type="gene ID" value="ENSPNAG00000001922.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000237581"/>
    <dbReference type="OMA" id="MDTRERC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050828">
        <property type="entry name" value="C-type_lectin/matrix_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45710:SF8">
        <property type="entry name" value="RERATING FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043343883" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="47"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005589.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="18989" checksum="FF776C20332D575D" modified="2024-11-27" version="2">TAYAIVALLFFSFLLVILMITLRAAASIPTNPPSNLEQVLEHGWTYFNYSLYHLSKEREPWTEARVACQHKNAELVIINSIEEQEFIQSLLTKSSSAWVGLTTMYSKWQWVDGTPETGISSTNDPPLSQCKNILKSEMRLMTEQCSNPHLWIFKLWAKYIVCTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C6Q5</accession>
    <name>A0A3B4C6Q5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pyroglutamyl-peptidase I like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547672.1">
        <property type="nucleotide sequence ID" value="XM_017692183.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C6Q5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006795"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002926.2">
        <property type="protein sequence ID" value="ENSPNAP00000006795.2"/>
        <property type="gene ID" value="ENSPNAG00000012782.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424270"/>
    <dbReference type="CTD" id="145814"/>
    <dbReference type="GeneTree" id="ENSGT00390000015368"/>
    <dbReference type="OMA" id="ANNCCIE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00501">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000816">
        <property type="entry name" value="Peptidase_C15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00706">
        <property type="entry name" value="PYROGLUPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006795.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26410" checksum="AD6D56C740F01FE9" modified="2024-11-27" version="2">MDSPQKELVVVTGFGPFRQYLVNASWEAAEGLQRAGLGEGIETHTVEIPVSYSKAQQAVAEIWQNMKPKFAVHLGIAPEAKCIILEQTAKNHGYKDRDVCGFSPANNCCIEEGLERLDSIICMKSLAKHFKGLGLDVIYSRDAGRYLCDFVYYCSLQYGQRRAALFHVPASGSLAHPETLVPQLQAIIQALLCQLDAYSISMQNQTGPREEECTRRHNTTNIVMCSGRLQRQWGPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CBB9</accession>
    <name>A0A3B4CBB9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein unc-119 homolog A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">UNC119B</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in synaptic functions in photoreceptor cells, the signal transduction in immune cells as a Src family kinase activator, endosome recycling, the uptake of bacteria and endocytosis, protein trafficking in sensory neurons and as lipid-binding chaperone with specificity for a diverse subset of myristoylated proteins. Specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated GNAT1 and is required for G-protein localization and trafficking in sensory neurons. Probably plays a role in trafficking proteins in photoreceptor cells. Plays important roles in mediating Src family kinase signals for the completion of cytokinesis via RAB11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PDE6D/unc-119 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559863.1">
        <property type="nucleotide sequence ID" value="XM_017704374.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CBB9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009232"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015514.2">
        <property type="protein sequence ID" value="ENSPNAP00000009232.1"/>
        <property type="gene ID" value="ENSPNAG00000014760.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431312"/>
    <dbReference type="CTD" id="678653"/>
    <dbReference type="GeneTree" id="ENSGT00390000014595"/>
    <dbReference type="OMA" id="QKITEHY"/>
    <dbReference type="OrthoDB" id="10248777at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070121">
        <property type="term" value="P:Kupffer's vesicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001287">
        <property type="term" value="P:negative regulation of caveolin-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900186">
        <property type="term" value="P:negative regulation of clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.40:FF:000001">
        <property type="entry name" value="protein unc-119 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.40">
        <property type="entry name" value="GMP phosphodiesterase, delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051519">
        <property type="entry name" value="PDE6D_unc-119_myristoyl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037036">
        <property type="entry name" value="PDED_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951:SF5">
        <property type="entry name" value="PROTEIN UNC-119 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951">
        <property type="entry name" value="RETINAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0844">Vision</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="16">
        <location>
            <begin position="125"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="284" mass="30822" checksum="20C13CF1D891C7BD" modified="2018-12-05" version="1">MSYSCTSTGNSQDPSSSNKSGGGVNPGSCSGSSSSGVSSSNSSSSSSVSSGGGGGGSSASHSVRGGKSDSKPASAEAMKVKKGCNTTDVGVPVTTEEELLANTIITPEDVLGLQKITENYLCSPEDNIYNIDFTRFKIRDMETGTVLFEITKPPASDKGGEKRDIDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDIPINNFRMIERHYFRDQLLKSFDFEFGFCIPSSKNTCEHIYEFPPLSEDIMREMILHPYETQSDSFYFVDNKLVMHNKADYSYSGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CCY3</accession>
    <name>A0A3B4CCY3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CCY3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009817"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016260.2">
        <property type="protein sequence ID" value="ENSPNAP00000009817.1"/>
        <property type="gene ID" value="ENSPNAG00000015300.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286980"/>
    <dbReference type="OMA" id="SKCYVFH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055077">
        <property type="term" value="F:gap junction hemi-channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055003">
        <property type="term" value="P:cardiac myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF106">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="148"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="301" mass="33842" checksum="C97E90F76982B95C" modified="2018-12-05" version="1">MTEWTLLKRLLDAVHQHSTMIGRLWLTVMVIFRLLVVAVAAEDVYADEQEMFVCDTLQPGCPNVCYDAFAPISQPRFWVFHIIAVSTPSLCFIIYTWHNLAKRACGLSCDSDSCSITSHRHLGHTPDGSSSGVLSKIYVCHVCFRAMLEVGFLVTQWLLFGFHVPAHFVCTIPPCSQRVDCYVSRPTEKTIFLVFMFSVGAFCIFLNILELNHLGWKKIKKSMNVKDGYGATNHDGRSMTSLTFREVTSTTSLPTLDLVLDHRPDWTCAGNCSPKDVHNPKGKKQPHKGKSTKSRGAEVWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CD76</accession>
    <name>A0A3B4CD76_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">COMMD1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the COMM domain-containing protein 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572000.1">
        <property type="nucleotide sequence ID" value="XM_017716511.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CD76"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008831"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015001.2">
        <property type="protein sequence ID" value="ENSPNAP00000008831.1"/>
        <property type="gene ID" value="ENSPNAG00000014415.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438599"/>
    <dbReference type="CTD" id="150684"/>
    <dbReference type="GeneTree" id="ENSGT00390000012029"/>
    <dbReference type="OMA" id="MPTAIVE"/>
    <dbReference type="OrthoDB" id="10251426at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000009">
        <property type="term" value="P:negative regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902306">
        <property type="term" value="P:negative regulation of sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04749">
        <property type="entry name" value="Commd1_MURR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033776">
        <property type="entry name" value="COMMD1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037351">
        <property type="entry name" value="Murr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21199">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21199:SF1">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17221">
        <property type="entry name" value="COMMD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="3">
        <location>
            <begin position="118"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="189" mass="21000" checksum="C0736355954FE36D" modified="2018-12-05" version="1">MAEADSVKALSGLLNGIAQKAYYGNSDITEDLLKSEIYPDMSQEEFHALHEKMRGVLKSIATADMDQTQLEAFLTAQTCKQGGGGVSSEQAAALGRFWKAHRMRIRESLLAQSRWEPGLRSVNWRVDLQTASSRGSTSSTPVALVELELGRNRQDSEFVCLEFDELKVNQVLKKMAEIQESIDSIVHHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CG04</accession>
    <name>A0A3B4CG04_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Serine/threonine-protein kinase PRP4 homolog</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">PRP4 pre-mRNA-processing factor 4 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts with CLK1 C-terminus. Associates with the U5 snRNP and NCOR1 deacetylase complexes. Identified in the spliceosome C complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CG04"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009811"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016280.2">
        <property type="protein sequence ID" value="ENSPNAP00000009811.2"/>
        <property type="gene ID" value="ENSPNAG00000015304.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155562"/>
    <dbReference type="OMA" id="PWLKKIN"/>
    <dbReference type="OrthoDB" id="3967at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14135">
        <property type="entry name" value="STKc_PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000078">
        <property type="entry name" value="Serine/threonine-protein kinase PRP4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000123">
        <property type="entry name" value="serine/threonine-protein kinase PRP4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044092">
        <property type="entry name" value="STKc_PRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF103">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PRP4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="17" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="669"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="135"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="198"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="254"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="417"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="461"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009811.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="989" mass="113717" checksum="4CCAB5DFFBBCBC75" modified="2024-11-27" version="2">MSEEDEEEEGEEQAEVNGENRREGSKHHGSSGSKHKKKKHKHRSKHKKHKHASEEEKERKRKHRHKHKKHRRKETTSPSVAAVNRKAEDASPSSENPNMDDRALLEDLEKQRALIKAELDSQLMEGKVQSGMGLILQGYNSGSEEDGEGQRARNGDQRQRSTGGKTRSPRDQSSKGGKARRESPDAGKASAKRRSRSKSREKSGKDSKAGKMTKSSRELAKERSKSKDRKCSRSRDRSKDRGRKSPSPPSRRRSSTERRNEQKGRSDRRSSPTRAEEKRSQEQGSRRSVSRRSRSRDRRSRSKDGHPARSEIDREKRPGKSPSKDASSGKENRSPHIRRPSSPQRRRSSSPRHRERQSHPPPSGSTERSSKQSHSPPRNRSPARRIRSRSGERRRDSPARKRSRADGIGRSHDSSPVRRRVSRSPLRRRSMSPRRQARPSPRRRSRSPLRRRSPERDRFGRPRPRRSTSRDRERRRRRSRDEDKFKGSLSEGMKADQDSSSEDVMEDFDAEEEDEEALIEQRRQQRLAIVQKYKAVNEDSNMGSMASEPSSPQSSTRSRSASPDDILERAAADVKEYERENVNTFEAIVKAKHNLIAQEKEGQSNTKKPSAPDMFTESDDMFAADFDSARLRAVGIGKDFKENPNLRDNWTDAEGYYRVNIGEMLDKRYDVYGYTGQGVFSNVVRARDTARAGQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPYLVSRFYRAPEIIIGKPYDYGIDMWSVGCTLYELYTGKILFPGSSNNHMIKLSMDLKGKMPNKMIRKGLFKDQHFDQNLNFLYIEVDKVTEREKVTVMSTINPTKDLLADLIGGQRLPEEQRKKVMQLKDLLDGTLMLDPAKRISINQALQHPFIQDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CI97</accession>
    <name>A0A3B4CI97_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein AMBP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">AMBP</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CI97"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011153"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018037.2">
        <property type="protein sequence ID" value="ENSPNAP00000011153.1"/>
        <property type="gene ID" value="ENSPNAG00000016836.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433551"/>
    <dbReference type="GeneTree" id="ENSGT00940000160109"/>
    <dbReference type="OMA" id="CPWLKRI"/>
    <dbReference type="OrthoDB" id="9949223at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22596">
        <property type="entry name" value="Kunitz_bikunin_1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22597">
        <property type="entry name" value="Kunitz_bikunin_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000005">
        <property type="entry name" value="Pancreatic trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002968">
        <property type="entry name" value="A1-microglobln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029856">
        <property type="entry name" value="AMBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46676">
        <property type="entry name" value="PROTEIN AMBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46676:SF1">
        <property type="entry name" value="PROTEIN AMBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01215">
        <property type="entry name" value="A1MCGLOBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0157">Chromophore</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017447546" description="Protein AMBP" evidence="13">
        <location>
            <begin position="19"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="227"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="14">
        <location>
            <begin position="283"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="347" mass="38748" checksum="B62DE1E675A3256C" modified="2018-12-05" version="1">MKSALVFLLLGFVALLQANPLPSQPVLQTQENFNLNRFLGKWYDIASASNCPWRKKYKGDSPIGSLELNRSDSSKALSMKRIMSRHGVCKMITGDYELTETPGRFYYHSVKWSADVDAYVVHTNYDEYALVVMLKQQQGGNKTTSVKLYGRKMELRPTLLDDFKALVAEQGMSADTISIKQNKGECIPGEQTTPKPQTQVRARRTAVLPAPEEGSGDMPMFRGAESCTAEKDSGPCFGMLQRYHYNSSLMACQKFIYGGCVGNQNNFMTEKECLQTCRTEAACRLPMDAGPCTTPQELWAFDSSSGKCVTFKYGGCKGNGNKFYSQKECEEYCGVMKDGDEEFLKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJC4</accession>
    <name>A0A3B4CJC4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CFAP53</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP53 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574423.2">
        <property type="nucleotide sequence ID" value="XM_017718934.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJC4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010614"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035780.2">
        <property type="protein sequence ID" value="ENSPNAP00000010614.2"/>
        <property type="gene ID" value="ENSPNAG00000016196.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440177"/>
    <dbReference type="GeneTree" id="ENSGT00970000193642"/>
    <dbReference type="OMA" id="IQAQHND"/>
    <dbReference type="OrthoDB" id="75950at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043596">
        <property type="entry name" value="CFAP53/TCHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31183">
        <property type="entry name" value="TRICHOPLEIN KERATIN FILAMENT-BINDING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="9">
        <location>
            <begin position="159"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="88"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="211"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="402"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010614.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="514" mass="62813" checksum="375519B0601E606F" modified="2024-11-27" version="2">MLMSQRHRARCREFTGPTPHSVAVRARYPSSRPPDYLILEQRKQETIRDKVLEFTKDQSACDLRMRWERNTEHRMVSATIDRRVQEAMAQYQMSIDEKRERLREMLESEESEFLREQEEKKETVLERQTKMRERARYLKETREHERQKIVADKLDQLFREQCEELRAAQTRRRQDEVCRERAAQIRSREEERHLHQGEEMLFAQLWESDRLAKEERENQQAQRQRESNFQTLAFLQAQMEAAEQQRLQAKQLKEEEAQILREQREMLRLEEQRGHMQKLQSQQSRRRQLDLCLRLKMKRVAQEQQDELALDMSILEQLLTHERDEKKEEALRKLELGEEQQKYRQYLAEQLEEQRRQEAETEKLIEAELQQTWRRRAEQWRLEKEARDRLMRDVLDIRRLQIQEKLERNAHKQAELAKESEELNRIIQENKQLDEQEKTCLREACQEYQTDLLAQMLHQQQIREAEKAEKEREFQRGLIYQEKYNQKIQDILSRPVSNTTAVHPFRRRDRPTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CK44</accession>
    <name>A0A3B4CK44_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CK44"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018789.2">
        <property type="protein sequence ID" value="ENSPNAP00000011730.2"/>
        <property type="gene ID" value="ENSPNAG00000035589.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160877"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000001">
        <property type="entry name" value="Serine protease inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF363">
        <property type="entry name" value="SERINE (OR CYSTEINE) PROTEINASE INHIBITOR, CLADE A (ALPHA-1 ANTIPROTEINASE, ANTITRYPSIN), MEMBER 1, LIKE PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="2">
        <location>
            <begin position="14"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011730.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="175" mass="19623" checksum="DBF41E0DFBCC749C" modified="2024-11-27" version="2">MSSLLAPHNADFTFSLYKKLAAHPEYQGKNIFFSPLSISMALSMLALGAKGETHSQIFSTLGYSTLTPDQVNEGYEHLLHMLGHRQDTMLLEAGSAVAVQEGFKPVDKFLKDAQHYYQGEAFSVDFSKPEVAVQEVNKYIAKKTKDMITDMVKELDPDTVMMLINFIYFRGTVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CKS3</accession>
    <name>A0A3B4CKS3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor HES-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Hairy and enhancer of split 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ATP6V1G2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CKS3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012627"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019951.2">
        <property type="protein sequence ID" value="ENSPNAP00000012627.2"/>
        <property type="gene ID" value="ENSPNAG00000018430.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163346"/>
    <dbReference type="OMA" id="LSHCEVQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097150">
        <property type="term" value="P:neuronal stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11461">
        <property type="entry name" value="bHLH-O_HES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000033">
        <property type="entry name" value="Transcription factor HES-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="14">
        <location>
            <begin position="23"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="15">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012627.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="156" mass="17779" checksum="866013FE03241DC5" modified="2024-11-27" version="2">MILTCYPTITAALISSKQHLPLTNKLRKPVVEKMRRERINNSIEKLKSLQGREFLKQQPDSRQEKADILEMTVHFLMQQQKQQQREQQSYNSVCSASASEGYGRCVQEAVIFLSQCGVQTPSQRRLLSHFLTMQPSMEKSTSVLLICSSVCNISSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRS8</accession>
    <name>A0A3B4CRS8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CEP170B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CEP170 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558741.1">
        <property type="nucleotide sequence ID" value="XM_017703252.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037398123.1">
        <property type="nucleotide sequence ID" value="XM_037542226.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000014802"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022825.2">
        <property type="protein sequence ID" value="ENSPNAP00000014802.1"/>
        <property type="gene ID" value="ENSPNAG00000020760.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430645"/>
    <dbReference type="CTD" id="792564"/>
    <dbReference type="GeneTree" id="ENSGT00940000157058"/>
    <dbReference type="OMA" id="VHVYILE"/>
    <dbReference type="OrthoDB" id="444265at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="CDD" id="cd22725">
        <property type="entry name" value="FHA_Cep170B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029300">
        <property type="entry name" value="CEP170_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF18">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15308">
        <property type="entry name" value="CEP170_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="23"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="555"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="664"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="751"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="822"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="892"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="982"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1530"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="751"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="924"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1003"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1039"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1065"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1092"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1125"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1176"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1210"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1263"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1302"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1577"/>
            <end position="1586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1586" mass="174671" checksum="BBAC961D78E837C4" modified="2018-12-05" version="1">MSVTSWFLVSSSGTRHRLPREMIFVGREDCELMLQSRSVDKQHAVINYDSAADEHLVKDLGSLNGTFVNDLRIPDQTYITLKLSDVVRFGYDSHVYIMERSQHKVPEEALKHEKYTSQLQMGLKALEAKKREQAEEKSRKPTESPCSKQGKSEGKSTTAAEPPVSKPTPLYGQPSWWGDVDENMGQQREPNAGNQKEDSRHEVNGSTSEHQDGQGKLVYSYQKDADYFEIPTKELHLRPKTAEPEVQVIPTKDTDNQAPSTPPVVQSHASFTIEFDECTPGKIKIKDHVTKFSFRQRKTPSKESVCAPTEVMSAESKVADWLVQSDANMARRKPRSEDLYSTKSDLSINRTILKDGHHEDGTQSDSEDPATKPPHVSKSDLSPPRLFTPPEREEPHASSTPHSQSQGRQEPQQAFVIEFFDDVAHKKRSQSFTNNISPPESHEPLKNRMEKMKSPSQTGEKSIPTQQFTVPLKDSGGFQRAGSLRREKTDIRMSSFNFSSRSASSKTFNSVGRRSKLAQDFAAEFLRASKPISTATWDKPPSSTSSLASNIVSMVTNNSSTPSQPPVPTSIQTKVSSPSHQQVPSKSLLTPTPANCTDVKNTKGSRQEEEDSLSDAGTYTIETEGPDKELEEARNKIDQVFGVGECPQHSSQPSAATVYRPVGRDREGQDSLPLSESSPGPVLGHYPGFSDPSIGPAQVESMILTTGSTRWVSRWASLADNYSDSGSVSGLFDIPAQMDLSADGRIIHQTMLNRNTDSTESESGQNSRGRRILPQVPSIEKTETLPPSIHIRSDSYLNYEGMERNIRGSQQKEDLQKLYVPDDLEPDSLSDASKSDDGSIVEQVRKSGAARPEKIGLEVKSQTSSKHEALASVAAKMLKDQDSTQKFSTATLTRQYGNKPNHAPPNRDASHCEENPVSLIRQESFTKDRSSDDIHFTRLPHISSPSSSNDKNAFKGVCSQDTQSYLKETENVLASLEARLQVQKRDSGPCPLEDSLSGESDVDTSSTVSQRSGKNAVTSAPKKPVILSELLKERKAASQHAQEQNRFQSPGDPLSQACRSSQADSDCKGESDKRFQLRRSSGKHGSLDFGSEPQSSTLRQWPETVSDQESNSRPTQRKFTIPLQKDNSSKSTKGTVAQALARSNSLSAPRPTRTSMLRRARLGEASDNDGTETDRTSQNSDANPSTTRPQESKKLSRLDILALPRKRAGSFTTPSDTESSAGKTGFSNRSSESGFSARKASISDQKTGAQKGSGGISRQPIIRGRSSSAKYASSTASSRRRQKGSDYTSTSEEEYESNQSTPKHKRSHHSGALHSLRTQNLLPVQPISRTRDSEGEGHESDAFQSWTTHSAEIARLSQDLAKDLAILAQEIHDVAGDAEPQNSASADTVEPVSAISTCEELRHQIPETSLNYPKVPPISAVVRNSDQIINDQSLKQCSWNQEQVALDNGMLNPVSQLSVAIRENTEQLTEKIKLLFHNKMKVWEEIEAKINTADDPPPLDTPNKEIMAILQDLRGIQKRLEVINRILEPSGNPDVAKSPVTGGQSTTGLRSFRVSPRDWRTTSSQRGGGPSASSRRFGREGESFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWD1</accession>
    <name>A0A3B4CWD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MRS2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Magnesium transporter that mediates the influx of magnesium into the mitochondrial matrix and regulates magnesium metabolism. Also permeable to calcium, sodium and potassium ions. Required for normal expression of the mitochondrial respiratory complex I subunits. May play a role in maintaining the inner mitochondrial membrane potential.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555918.1">
        <property type="nucleotide sequence ID" value="XM_017700429.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWD1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014844"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022848.2">
        <property type="protein sequence ID" value="ENSPNAP00000014844.1"/>
        <property type="gene ID" value="ENSPNAG00000020806.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428998"/>
    <dbReference type="CTD" id="57380"/>
    <dbReference type="GeneTree" id="ENSGT00390000009988"/>
    <dbReference type="OMA" id="TRNNCII"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000007">
        <property type="entry name" value="Magnesium transporter MRS2 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000003">
        <property type="entry name" value="Magnesium transporter MRS2 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF0">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="375"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="432" mass="48989" checksum="01052325BDA3C3F0" modified="2018-12-05" version="1">MEACVRLLCRESPLTVSTRCWTRRTFTLKRSRSPFTRPLSVRHSPLVWGGKHRGVGPGEALKHSSLFCRGRVTDASHAAALSSVAPEFVVMKFEPDGNVTSFEKKKTEFYQELGLQARDLRFQHLTSITSRNNTIIIRMESLKAVVTPDCLLVLDFRGLGLEKWLVLELGPQLAGDGALATYSLPFEFRALEAILQHRINTLRARLNEVQPQILDCLESLVDPKLLSADRSKLHMLLLNSKSLSELETDIKVFKDSLLKILDEDELIEELCLTKWTDPRVFEQSSLGIDHAHEMELLLENYFMQAEELGNKTRELKGLIDDSESVIFINLDSHRNVMMRLNLQLTMGTFSLSLFGLMGVAFGMNLESSFEEDPRVFWLVTGFMFLGSGLIWRRLLSFLGRHLEPTSPPPIPPVWRKNQIGTLKGTEIKPGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CWW0</accession>
    <name>A0A3B4CWW0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Vasoactive intestinal peptide receptor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">VIPR2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CWW0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015024"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023093.2">
        <property type="protein sequence ID" value="ENSPNAP00000015024.1"/>
        <property type="gene ID" value="ENSPNAG00000021029.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158089"/>
    <dbReference type="OMA" id="IWIAFRI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004999">
        <property type="term" value="F:vasoactive intestinal polypeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001571">
        <property type="entry name" value="GPCR_2_VIP_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002284">
        <property type="entry name" value="GPCR_2_VIP_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF22">
        <property type="entry name" value="VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00491">
        <property type="entry name" value="VASOACTVEIPR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01155">
        <property type="entry name" value="VIP2RECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="14">
        <location>
            <begin position="12"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="97"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="45302" checksum="A5800A0D3911E386" modified="2018-12-05" version="1">NFYQELQRAKEECLTRLNQQQSSGCKGFWDNISCWDGAEVGQSVTIPCPPVLKTLFNRNGNISKNCTVDGWSDVFPNITSVCGSETPQDKLVFYMVVKTLYTLGHSLSLIVLTTGSAILCMFRRLHCTRNYIHVNLFLSFILRAVAVLVKDSVLFSHSTHCSNQPSLVGCRASLVFFHYFIMANFFWLLVEGLYLHTLLLVTFAESTRFLSYLMIGWGARSLSSNSVQLPIQLISTHCWERNDHPVPIWVINGPIVFSMMVNFVLFISIIRILLKKLRCPDVGGNDQSQYRRLAKSTLLLIPLFGIHYVIFVSLNESLVDYKIFFDLALGSFQVGLLCVSCLMCQVQGELRRKWQSVWLNCQIWNYISQNASVLRNGSEAVMSEGRSRTASALQTETTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYV5</accession>
    <name>A0A3B4CYV5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYV5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026063.2">
        <property type="protein sequence ID" value="ENSPNAP00000017272.2"/>
        <property type="gene ID" value="ENSPNAG00000033146.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163148"/>
    <dbReference type="OMA" id="VMMRTIN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF119">
        <property type="entry name" value="GAMMA-CRYSTALLIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="12"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="98"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="139"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017272.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="185" mass="22308" checksum="C2477D1DAA1B5E58" modified="2024-11-27" version="2">MHITTHSAFYSMGITFYEDRNFQGRSYETSSDCAELTSYLSRCNSCRVQSGCFMVYDRSNFMGNQYFVRRGEYSDYQHMGLSDCFRSCRMIPMHRGPYRIRIYQRDNFGGQMYEIMDDCDSFMERYHMSDIQSCHVMDGHWLMYEQPHYRGKIAYLRPGEYRSYREMGLSSNMRFRSMRRIMESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D057</accession>
    <name>A0A3B4D057_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methyltransferase like 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">METTL22</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D057"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016943"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025578.2">
        <property type="protein sequence ID" value="ENSPNAP00000016943.2"/>
        <property type="gene ID" value="ENSPNAG00000023128.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048539"/>
    <dbReference type="OMA" id="AYERIQQ"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038899">
        <property type="entry name" value="METTL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108:SF0">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016943.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41510" checksum="DD6674A427F66675" modified="2024-11-27" version="2">MDQVAFRSDTVLSDVHLLLPNARHLMARLNSVGQPVFVSKFRILREEEQVEVQDSVKSDLGVLLDEDGDLVVTRKPNRMLCDREKIHPVILSQAGEAVSDEEDDEAEDKECAKDIIRIEHTMATPLEDVGKQIWRGAFLLSDFILSHPAVFQEATVLELGAGTGLASIIMARVAKKVYCTDVGEDLLSMCQRNVALNSHYTEPAAERGVKVRQLDWTADDFLTDADAEFGWSEEEVADLHDNTTFVIAADVCYDDDLTDALFQTLYRITSNLRSPSTAYFSIEKRLNFTLRHMDVSCEAYNHFRLCLKQLADMKDGKMKFTVEPVPCSFPQSFQYERVDQLVRLSASGCMDGCMERWVCGWMNGWMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0G7</accession>
    <name>A0A3B4D0G7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Gamma-aminobutyric acid (GABA) A receptor, alpha 6a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017567045.1">
        <property type="nucleotide sequence ID" value="XM_017711556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0G7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017090"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025786.2">
        <property type="protein sequence ID" value="ENSPNAP00000017090.1"/>
        <property type="gene ID" value="ENSPNAG00000023320.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435602"/>
    <dbReference type="CTD" id="393704"/>
    <dbReference type="GeneTree" id="ENSGT00940000156722"/>
    <dbReference type="OMA" id="QVVYFHL"/>
    <dbReference type="OrthoDB" id="203862at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000043">
        <property type="entry name" value="Gamma-aminobutyric acid receptor alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022255761" description="Gamma-aminobutyric acid (GABA) A receptor, alpha 6a" evidence="18">
        <location>
            <begin position="19"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="20">
        <location>
            <begin position="28"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="21">
        <location>
            <begin position="242"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="49304" checksum="4810DE80E7F5DACF" modified="2018-12-05" version="1">MGRILALLSLMCLGDVLGNVKISSENITRILDRLLEGYDNRLRPGSGVSVTEVKTDIFVTSFGPVSDVEMEFTMDMFFRQMWVDERLAFEGPIEILRLNNRMVDKIWTPDTFFRNARKSLAHNTTTPNKMFRIMQNGTVFYTMRLTVSAECPMKLMDFPMDGHICPLRFGSYAYTNREIVYTWRKGLVESVDVPPESSNLLQYDLIGKTLSSETLKISTGHYSVQLVHFILQRKLGYHLIQTYIPLIMVVVLSQVAFWINKESVPARTVAGVTTVLTMTTLSISARSSLPKVAYATAMDWFIAVCFAFVASALVEFAAVNYFATLEANRMRRRLSRGSVLESIAEGSDDEDESTVSDPSVGGRVRKRMSSISEAPKTRLPIFLQQGSATPPNVTLAGPSPIDQYARMLFPLAFAVFNIIYWYIYLSKDTIEKAREID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0P9</accession>
    <name>A0A3B4D0P9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aquaporin 1a (Colton blood group), tandem duplicate 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574170.1">
        <property type="nucleotide sequence ID" value="XM_017718681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0P9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038409.2">
        <property type="protein sequence ID" value="ENSPNAP00000017128.2"/>
        <property type="gene ID" value="ENSPNAG00000023354.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439997"/>
    <dbReference type="GeneTree" id="ENSGT00940000157015"/>
    <dbReference type="OMA" id="IKLWSFW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035379">
        <property type="term" value="F:carbon dioxide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015168">
        <property type="term" value="F:glycerol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003097">
        <property type="term" value="P:renal water transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF161">
        <property type="entry name" value="AQUAPORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017128.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="264" mass="27824" checksum="D535E06B4313C3EB" modified="2024-11-27" version="2">MVRELKTLSFWRAVLAELFGTAMFVFIGILAMIGNGNSSYPDQEVKVALAFGLAALSLCQSLYNISGAHLNPAVTLGMVVSCQVSVCRAVWYMLAQILGGVLASGVVLGVRPAAVESLGLNKLNGVSPGQAFGIEFLLTLQLVLCFLATTDKKRTDVFGSAPFAVGLSVGLGHLAGISYTGCGINPARSFGPALVTVAFENHWVFWAGPMCGGVVAALLYDFVLCPRGYDFAGRLKVWCHGVESLYGEPEPLLEGAGGEPSEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D135</accession>
    <name>A0A3B4D135_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein sprouty homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SPRY1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sprouty family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565215.1">
        <property type="nucleotide sequence ID" value="XM_017709726.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D135"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016776"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025346.2">
        <property type="protein sequence ID" value="ENSPNAP00000016776.1"/>
        <property type="gene ID" value="ENSPNAG00000022949.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434528"/>
    <dbReference type="CTD" id="10252"/>
    <dbReference type="GeneTree" id="ENSGT00950000183055"/>
    <dbReference type="OMA" id="YERESQP"/>
    <dbReference type="OrthoDB" id="10038884at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040037">
        <property type="term" value="P:negative regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007875">
        <property type="entry name" value="Sprouty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051192">
        <property type="entry name" value="Sprouty_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12365:SF10">
        <property type="entry name" value="PROTEIN SPROUTY HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12365">
        <property type="entry name" value="SPROUTY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05210">
        <property type="entry name" value="Sprouty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51227">
        <property type="entry name" value="SPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="339" mass="36529" checksum="C8A91221798650D6" modified="2018-12-05" version="1">MDRRGHRGATGSVAVIREPGGERLDPDLPAGAILSLEQIRAIRSSNEYTEGPAVAARRPPAPAPRLNDKHERTHEVILVNVNNNYEARPTATRPPALSRSTSMGSAGSSGSSGSASSEQGLLAQSPPSRHQHHRHHHHHHNNRLEQAIRTQPKSLSSPMQPLLPVDALLKQPVGFGPPLAGKGDLSPSSPHQFICERCGKCKCSECTAPRMLPSCLACNGQCLCSAESLVEHGTCMCLVKGLFYHCSNDDDDGDSCADRPCSLSRPRCLPRFLCMGLMAPLFPCLLCYPPAKACVKACQHLHDRINRPGCRCKNSNTVYCKLQGWGQGQGHGLGQGKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D1H4</accession>
    <name>A0A3B4D1H4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017577725.1">
        <property type="nucleotide sequence ID" value="XM_017722236.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017577726.1">
        <property type="nucleotide sequence ID" value="XM_017722237.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D1H4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016719"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025279.2">
        <property type="protein sequence ID" value="ENSPNAP00000016719.1"/>
        <property type="gene ID" value="ENSPNAG00000022937.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442267"/>
    <dbReference type="CTD" id="64844"/>
    <dbReference type="GeneTree" id="ENSGT00940000178451"/>
    <dbReference type="OMA" id="RMMSGNR"/>
    <dbReference type="OrthoDB" id="2154780at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16812">
        <property type="entry name" value="RING_CH-C4HC3_MARCH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052297">
        <property type="entry name" value="RING-CH-type_E3_ubiq-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14471:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14471">
        <property type="entry name" value="MARCH7/10 E3 UBIQUITIN PROTEIN LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="10">
        <location>
            <begin position="603"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="42"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="461"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="739"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="259"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="360"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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    </feature>
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        <location>
            <begin position="485"/>
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        </location>
    </feature>
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            <begin position="498"/>
            <end position="513"/>
        </location>
    </feature>
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        <location>
            <begin position="515"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="759"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="772" mass="84920" checksum="41A1445CBD825351" modified="2018-12-05" version="1">MDSKPRRLPFTISSSSSLSSPSSLSSSSSALSASRLYGRGSVLGSDRFSRGGAGKLDSDYQSPCLSGGPRDYSSSESRHSRWKLSTPLSSSSLSSSSSCDRQWTESSYGGRSKHVDSERGLGSYSSLLGAPQDSESKRAKLSYTNRATYSRSPFTSTPSSTYSNLGGLPDSSWKSTFSPLSRSSSSSSSSSSSSSSEGLWARREAEKRVDPGLTSLGEHHSYRPTSLTSSLYRPERVTSTYAQGARPKESLYSSRRESSSTSHRLSSEYLSSSLERSSHRLASDYQPSPRLPRDTPSSSATRTSVSRSDPLCLSSWSSSPYTALNECTSSPPPSRPAPVPNPAPEGGDSDGRSTTRRLLSRLFSRRSNQESSSTSSTNSVSRLFDTTPEEAPHSDAPPTVSRESVEADPAQAFAFLRRREQALSPVQENTDVEPEAESLRPPTGLSWLNWNRCTPLFSRRRREGRDESARMEARQSPPFPLSSPEGRKTPDRDTHHDDEEEEEEEEEEDDDVSEGATAAPPAGASGLSSALLQDASRLQGRSPGVTRVLSSPLFRMPENVIVGLDVGIEGRSQAEAQAKEKPAPSRDPERLRKIQESLLLEDSDEEEGDLCRICQMGETSSSNPLIEPCRCTGSLQYVHQDCIKKWLRSKISSGSTLEAITTCELCKEKLHLNIENFDINELYRSHERSEYEFISCGLYLVVLLHLCEQRFSDVLGAVNEAGLFNLARTLHEHMDNLESSYAESDEDEVQDSRPSIDFCDLEDEEEEEEEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D1N7</accession>
    <name>A0A3B4D1N7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PHF24</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017552714.2">
        <property type="nucleotide sequence ID" value="XM_017697225.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D1N7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025621.2">
        <property type="protein sequence ID" value="ENSPNAP00000016981.2"/>
        <property type="gene ID" value="ENSPNAG00000023164.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427229"/>
    <dbReference type="GeneTree" id="ENSGT00390000002865"/>
    <dbReference type="OMA" id="KGHIEWQ"/>
    <dbReference type="OrthoDB" id="9978298at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031946">
        <property type="entry name" value="KIAA1045_Zf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46453:SF4">
        <property type="entry name" value="PHD FINGER PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46453">
        <property type="entry name" value="PROTEIN KINASE C-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16744">
        <property type="entry name" value="zf-RING_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="302"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016981.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="410" mass="46448" checksum="0221CDE9915AF5BC" modified="2024-11-27" version="2">MGVLMSKKQQVEKVQKCSVVVSAFREGLKNQSSSVQAHNGAENAGDEALEQLDTEESGHKEDEGKPGPSSAQPASAPTREENKVNQEAWGRLRDGKGVEPEELKKAHQLTPPAFVRPKRQPNDDQPLQIELGQREQPVNDEMCEVCEVWTADDLFPCRTCTRVFHDGCLREIGYLRADALQEMRETAHTTTGWSCYYCDNVNLLLTEEEMYSLMETFKQCKIIPESCLVSDELLQYRHFVSKQLFEKDLTDEQEEEVLAQFAALDPEKKGQIEWSDFLYHETLSVLQKFRTENSLVRLLTAKERDRARAIFQSLDQDKDGVITGGESRWAQASWFHKISKESQSCNVSISHVGPISESSPASSSSDKSRDKAMEKYDNRPVTWDAFLRESAIYILAARPNSAAMHIRLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3R3</accession>
    <name>A0A3B4D3R3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cystinosin, lysosomal cystine transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CTNS</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cystine(out) + H(+)(out) = L-cystine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:66172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:66173"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystinosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569791.1">
        <property type="nucleotide sequence ID" value="XM_017714302.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017569792.1">
        <property type="nucleotide sequence ID" value="XM_017714303.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017569793.1">
        <property type="nucleotide sequence ID" value="XM_017714304.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3R3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017524"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026358.2">
        <property type="protein sequence ID" value="ENSPNAP00000017524.1"/>
        <property type="gene ID" value="ENSPNAG00000003785.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437293"/>
    <dbReference type="CTD" id="1497"/>
    <dbReference type="GeneTree" id="ENSGT00390000005338"/>
    <dbReference type="OMA" id="WIDVIYT"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000016">
        <property type="entry name" value="Cystinosin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000022">
        <property type="entry name" value="Cystinosin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00951">
        <property type="entry name" value="2A43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017249323" description="Cystinosin, lysosomal cystine transporter" evidence="12">
        <location>
            <begin position="24"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42730" checksum="EC2748DA68E2AFFF" modified="2018-12-05" version="1">MMNTGHSAAVFLALCALLIRCDAKVSLSALHTVDLEEKFSKNITITASFYLNTTANIYFNITYSSKNDSSSIIHLPKEVVMSAGSTSATFEVQGENVGQVTVYLYSNNTDIESIKTRIRFLVVRSYALAVINQIIGWIYFIAWSISFYPQAYENWKRKSVVGLNFDFLALNLTGFISYSVFNVGLFWIPYIKEEFLKKDPNGVNPVDANDVFFSLHALALTLVYVCQCTIYESGGQKVSKVAMILLGIAWTFAFVTLFVAVAQKITWLQYLYYFSYIKLGVTLVKYIPQAYMNYQRQSTEGWSIGNVLLDFTGGSFSLLQMFLQSYNNDQWRLIFGDPTKFGLGVFSIFFDVIFIIQHYCLYRSKAPEYRILGSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4W0</accession>
    <name>A0A3B4D4W0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18 26">Postsynaptic cell membrane</location>
            <topology evidence="18 26">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561386.2">
        <property type="nucleotide sequence ID" value="XM_017705897.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037400704.1">
        <property type="nucleotide sequence ID" value="XM_037544807.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000017939"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026936.2">
        <property type="protein sequence ID" value="ENSPNAP00000017939.2"/>
        <property type="gene ID" value="ENSPNAG00000005556.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432208"/>
    <dbReference type="CTD" id="100003342"/>
    <dbReference type="GeneTree" id="ENSGT00940000156964"/>
    <dbReference type="OMA" id="DIYVEHQ"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017146">
        <property type="term" value="C:NMDA selective glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004972">
        <property type="term" value="F:NMDA glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098655">
        <property type="term" value="P:monoatomic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06378">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13718">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000026">
        <property type="entry name" value="Glutamate ionotropic receptor NMDA type subunit 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000020">
        <property type="entry name" value="Glutamate receptor ionotropic, NMDA 2B, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000007">
        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018884">
        <property type="entry name" value="NMDAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10565">
        <property type="entry name" value="NMDAR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 26" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 26" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 26" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 26" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 26" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="13 26" id="KW-0675">Receptor</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 26" id="KW-0732">Signal</keyword>
    <keyword evidence="9 26" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 26" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043098619" description="Glutamate receptor" evidence="26">
        <location>
            <begin position="31"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="639"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="828"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="28">
        <location>
            <begin position="451"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="29">
        <location>
            <begin position="458"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1090"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1361"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="521"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="697"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="698"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="739"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="24">
        <location>
            <position position="670"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="24">
        <location>
            <position position="703"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="753"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017939.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1415" mass="158522" checksum="F129324ED18B62F7" modified="2024-11-27" version="2">MGVRLGRLSDTLWPPLLLLLLLCALPSCQGRPFTMVEPTVNVAVVFSGSAYQLEIKGRLSRENFLDLPMEVNPITVLVNNTNPRALLTRICDTLAANRVHGVVFEDNIGSEAVAQILDFISTQTAVPIVGISGGSAVVLPHKGDGSTFLQLGSSIEQQINGMFKVMEEYNWDSFVVVTSQYPGYEAFVDYIRSFTDTSYFLWELQDVLTFDMSADGMNDLRARRLLQQIDAQVLLVYCSHDEAQYLFSMAKEVGLLGPGYIWIIPSLAVGNPNIPPPDSFPVGLISIITDRWKMSLRNRVRDGVAIVVKGVESFRKHRGFIPDGHTDCQTPLRPDTNDTLFKHMLNVSWEHTDFSFNSDGYLVNPSMIIITLDRERQWDKVGSYESGILQMRYPVWPRYGTYMEPVSDNRHLTVATLEERPFVIVEAVDPITGTCLSNTVPCRRQSNKTETIIGHTEPYTKLCCKGFCIDILKKLSRNIKFSYDLYLVTNGKHGKLVRGVWNGMIGEVVYKRADMAIGSLTINEERSEIIDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAVTVFVFEYFSPVGYNRSLVSAKDPGGPTFTIGKSVWLLWGIVFNNSVPIENPKGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEQYIDTVSGLSDKKFQKPQEQYPPFRFGTVPNGSTERNIRSNYPEMHSHMVKYNQKGVEDALNSLKTGKLDAFIYDAAVLNYMAGKDEGCKLVTIGSGKVFATTGYGIALQKGSRWKRPIDLALLQFLADGDTAKLQTVWLTGICRNEKKEVMSSKLDIDNMAGVFYMLLVAMGLSLLVFAWEHLLYWKLRHSVRKSERLDFLLAISRGIYSCFNGVEDTDRNGSMQSPDVTTNYTQANMLKMLKTAKDIVSSARVENSLDNATKTIENLSRRGPDATKPCLPTRLISTDSSHGRLPYISSVTDNHAPYPHTPTFPLHYGDSGTQPLLEKPRVVTRPTPLRYTLPARTSHHHIYERTLPISSLSSPHIAMRDPPPPTAPKPHHSGRFYMDSQACHQTSPFLPYREFHVPDIYVEHQEPSGSRKLYAEGLGRKKRSHSYVAPSPETRRRNDYDEPTSCLAELRANHLLDNHAHTTPCMGSHYQRPSGSCNSCMKPYLDPEMDDVPLLGKEKLNRRASFLRATWGGDRIRHLDEKPSTSASSSSRINMPDLFPRVVVANPKPSKMYSSAGNNLCYNMAVEPAYLDPAHMGSYPYKPQKLKYSQSTRLPSYREAILQNAAALRRSSSTVVHRHYSTYLNSYTDLPVYVGPLAQGPAHFYDHSKDMCHLFPCVNHCPDDAALLPRLSGRPVMYQNTAFGGYEGAGRSRDEPPLQPLHTLPASSGNRDRRQVLVARRVNSPYVPKPWGRVSSLESEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5F9</accession>
    <name>A0A3B4D5F9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger, AN1-type domain 5a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017565132.1">
        <property type="nucleotide sequence ID" value="XM_017709643.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017565133.1">
        <property type="nucleotide sequence ID" value="XM_017709644.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017565134.1">
        <property type="nucleotide sequence ID" value="XM_017709645.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5F9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018149"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027237.2">
        <property type="protein sequence ID" value="ENSPNAP00000018149.1"/>
        <property type="gene ID" value="ENSPNAG00000024513.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434484"/>
    <dbReference type="CTD" id="406312"/>
    <dbReference type="GeneTree" id="ENSGT00940000164966"/>
    <dbReference type="OMA" id="QQPHIND"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4770:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF26">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="9">
        <location>
            <begin position="148"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23153" checksum="1BDCFF79430D0B25" modified="2018-12-05" version="1">MAQETNQSPVPILCTTGCGFYGNPRTNGMCSVCYKEHLSRQQSSDRSPVSPLAGSPSSEATAIQRLEASLNKTEPSSAANIESMRENMPAASLPVTQQMTEMSISREEKNPSPKPDTVEPVVTQPTSSYSPVAGAQASDEGKSPDASKPKKNRCFTCRKRVGLTGFDCRCGNLFCGIHRYSDKHNCTYDYKAEAAAKIRKENPVVVADKIQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DBJ4</accession>
    <name>A0A3B4DBJ4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017559351.1">
        <property type="nucleotide sequence ID" value="XM_017703862.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DBJ4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021787"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033411.2">
        <property type="protein sequence ID" value="ENSPNAP00000021787.1"/>
        <property type="gene ID" value="ENSPNAG00000028939.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431004"/>
    <dbReference type="GeneTree" id="ENSGT00940000154821"/>
    <dbReference type="OMA" id="DIYLLCH"/>
    <dbReference type="OrthoDB" id="784962at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000004">
        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bromodomain-like"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF22">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="106"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="224"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="268" mass="30381" checksum="B4FA2D3975155B3E" modified="2018-12-05" version="1">MKRLSARCNAGLLILSPPDKRQPGDHSQSQAIQNGVSEDMEEESSLKKRKSDHQGNQASETGQEVVEKVKKRRGRPPAEKLPPNPPKLTKQMNTLVDMVMNYKDTLGRQISKGFVQLPSRKEVPEYYELIRKPVDFRRIRERVRNHKYRCIADLEKDIIQMCHNAQTYNLEGSQIFEDSIVLKSVFESARQRIVEIHQEDNDAVGGSEADCADHLNLELKSEATNTKFDKNRERSKTAPANSSAKVKDIYSDEDSAEDQGDNTGLEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DC76</accession>
    <name>A0A3B4DC76_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 25 member 36</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574829.1">
        <property type="nucleotide sequence ID" value="XM_017719340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DC76"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020544"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040088.2">
        <property type="protein sequence ID" value="ENSPNAP00000020544.1"/>
        <property type="gene ID" value="ENSPNAG00000027426.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440473"/>
    <dbReference type="GeneTree" id="ENSGT00940000154369"/>
    <dbReference type="OMA" id="IACLIAY"/>
    <dbReference type="OrthoDB" id="269120at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 25 member 36"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF2">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="116"/>
            <end position="203"/>
        </location>
    </feature>
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        <location>
            <begin position="224"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="311" mass="34324" checksum="8639070D9DA8C939" modified="2018-12-05" version="1">MSQRDTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSSAITLYISSVQLSTVSGAGVARMAPPGPLHCLKLILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAYSTAKERLNGLLVPDSTQVHMLSAGIAGFTAITATNPIWLIKTRLQLDARNRGEYRMSAIECVRRVYQTDGLRGFYRGMSASYAGISETVIHFVIYENIKQRLLQARAATHMDDEDMTTRDASDFVGMMLAAATSKTCATCIAYPHEVIRTRLREEGTRYRSFFTTLSTVCREEGFRALYRGLTTHLVRQIPNTAIMMSTYELVVYLLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEJ5</accession>
    <name>A0A3B4DEJ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane and ubiquitin-like domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TMUB1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017541228.1">
        <property type="nucleotide sequence ID" value="XM_017685739.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEJ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021481"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032809.2">
        <property type="protein sequence ID" value="ENSPNAP00000021481.1"/>
        <property type="gene ID" value="ENSPNAG00000028556.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413293"/>
    <dbReference type="CTD" id="83590"/>
    <dbReference type="GeneTree" id="ENSGT00390000014069"/>
    <dbReference type="OMA" id="TLLWYCQ"/>
    <dbReference type="OrthoDB" id="161999at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17131">
        <property type="entry name" value="Ubl_TMUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040352">
        <property type="entry name" value="TMUB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557">
        <property type="entry name" value="PROTEIN C7ORF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="12">
        <location>
            <begin position="142"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="34"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="285" mass="30583" checksum="81520A9F7FBE6A87" modified="2018-12-05" version="1">MTLIEGVGDEVTLLFGVVFLVLVVVLAWASTHTAEPPEHLLPSTPAPAAPSSEAESQEPLPSSDITSPSHSLRDDGKAEAESGAEGQASDEGGGDRGLLGADGLRHREAAGPSLATQPPASASGPTQPTSSEDVSNDTVRNMVLRLKFLNDTERIAQVNPEDTIGYIKRTYFAGQEHQVRLIYQGQLLQDDAQTLSSLNLADNCVLHCHISQHATRAVPAGARAADQVHVALNVGSLMVPLFVLMLSVLWYFQFQYRQFFTAPATASLVGITIFFSFVAFGVYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DF98</accession>
    <name>A0A3B4DF98_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 20">Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase</fullName>
            <ecNumber evidence="3 20">2.4.1.135</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">B3GAT1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 20">
            <text>3-O-(beta-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24168"/>
            <dbReference type="Rhea" id="RHEA-COMP:12571"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132090"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="EC" id="2.4.1.135"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Golgi apparatus membrane</location>
            <topology evidence="20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.135" evidence="3 20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DF98"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022180"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034171.2">
        <property type="protein sequence ID" value="ENSPNAP00000022180.2"/>
        <property type="gene ID" value="ENSPNAG00000029477.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157165"/>
    <dbReference type="OMA" id="RTEYAYG"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00218">
        <property type="entry name" value="GlcAT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000010">
        <property type="entry name" value="Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF50">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 17 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="7 20" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043411236" description="Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase" evidence="21">
        <location>
            <begin position="32"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="15">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="91"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="195"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucuronate</name>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="18">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="18">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="19">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022180.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="334" mass="38148" checksum="08FBBE296FA49B21" modified="2024-11-27" version="2">MPRRRDVLALVLIVLPWTLLITVWHQSPVTPQPVTRKDNRRVVSKTHSNGLQPKESCVIYNSVSGVVRTEYAYGRPQPWSDLLPIIHIITPTYTRPVQKAELTRLANTLLHVPNLHWIVVEDAPRRTALVSRLLRSTGLNHTHLNVETPRAYRLHREPRAARVPRGTTQRNLGLRWLREALGHGAENRGVVYFADDDNTYSLELFEEMRSTQKVSVWPVAFVGGLRYESPKINTMGKVYGWRTVFDPHRPFAIDMAGFAINLQLILSKPQAYFKLKGVKGGYQESSLLRELVTLGDLEPKADNCTKVLVWHTRTERPVLVNEGKRGFTDFSVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFD9</accession>
    <name>A0A3B4DFD9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCLAF1/THRAP3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571410.1">
        <property type="nucleotide sequence ID" value="XM_017715921.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017571411.1">
        <property type="nucleotide sequence ID" value="XM_017715922.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFD9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037252"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033116.2">
        <property type="protein sequence ID" value="ENSPNAP00000021634.1"/>
        <property type="gene ID" value="ENSPNAG00000028762.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438233"/>
    <dbReference type="CTD" id="407984"/>
    <dbReference type="GeneTree" id="ENSGT00950000183163"/>
    <dbReference type="OMA" id="GEGTDKW"/>
    <dbReference type="OrthoDB" id="9948513at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029199">
        <property type="entry name" value="THRAP3_BCLAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15268">
        <property type="entry name" value="THRAP3/BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15268:SF16">
        <property type="entry name" value="THYROID HORMONE RECEPTOR-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15440">
        <property type="entry name" value="THRAP3_BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="391"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="635"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
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    </feature>
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        <location>
            <begin position="112"/>
            <end position="124"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
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            <begin position="125"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="261"/>
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    </feature>
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        <location>
            <begin position="362"/>
            <end position="374"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="445"/>
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    </feature>
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        <location>
            <begin position="446"/>
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        <location>
            <begin position="666"/>
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        <location>
            <begin position="696"/>
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        </location>
    </feature>
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        <location>
            <begin position="729"/>
            <end position="771"/>
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    </feature>
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        <location>
            <begin position="780"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="819"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="881"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="900" mass="102795" checksum="19D9A16B9FEF2CC0" modified="2018-12-05" version="1">MSKPPNSPSRGSRSRSRSRSRSYSRSRSSSRSRSRSRKHRYSSRSRSRSRSRDRERNYPTRDFQGNHGYTRGFRGYRRPFYHNRGRGRGYFPRGYHRGGGNYGYRSNNWHGGHRDQQQSYEHHSHSPKRGRSRSRTPRKRSGSRSRSRYSDRSSSRSRRSSSSSRSSSPRRRSSGKPHSKDSKSKGSPGEGKGPNKEEDIKPSAAVPEEPPSKWESLSDYGTSPKQPNQGQTSAGAQPEVKVSLTGASGNGASLWRSIDGSKSPPKQASSSSGFGFFSKEDMKAGDKSAQISTAFKKFLAEKKKPSSEWDNGQDEDVNSGDAEKEKSRKQRGLFDVEPAYGEAKPDKGLPFLDEEEEAYLKSLKERKTEDESKYKPKGTLSARELFEERFGKWDDDSIASNKDSAQREEEVDEDENVMEELYRSRKQAARKEEKASKKKEKKKNRVSPSSPSPSRSTERGRPLFPAAREVSPPARSSKKKDPEFNFSLKTFNEEAESSSGALAKERRLTQDLVHPVKKEHEEFRSIFQHIQAAQLRRSPSELFAQHIVTIVHHIKAQHFRSSGMTLNERFGMYQRKAAQMEMMRQKKSPEIHRRIDVSPSAFKKHFRQFEEMEESEYKDYGKKYEGESMDLRLDIERRKKYPKREGGKGSAGSRTPSHEQSPDKPSKHKKSKKSKKKRERSPSSSSSSSPSPHPYRPREYHGEEVEHVEKGGFDKSRLGPREYPGLIDRGPRDYEGGHLERGYERGRGGYDRGGYDRAGYDRGRGGYDRGFPRMRGRGWNRGNYPGNNNNGNPANMGGPIRPQDEEWDPEYTPKSRKYFQHDDRDREGEMKWTDGRGRGRGLYPRSRGSFTVRRGSSGSSPKWTHDMFQGATEEGELPDDGTDHSHKEEDKTAESETSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFM8</accession>
    <name>A0A3B4DFM8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-hydroxytryptamine receptor 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serotonin receptor 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR4 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via C-terminus 330-346 AA) with GRK5; this interaction is promoted by 5-HT (serotonin).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFM8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021714"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040746.2">
        <property type="protein sequence ID" value="ENSPNAP00000021714.2"/>
        <property type="gene ID" value="ENSPNAG00000028860.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182934"/>
    <dbReference type="OMA" id="WMPFFTV"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032098">
        <property type="term" value="P:regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15055">
        <property type="entry name" value="7tmA_TAARs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000030">
        <property type="entry name" value="trace amine-associated receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001520">
        <property type="entry name" value="5HT4_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF66">
        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01059">
        <property type="entry name" value="5HT4RECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="36"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021714.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="353" mass="39573" checksum="2171E40E626230D5" modified="2024-11-27" version="2">KPKCSVNILSVSGKACVKSLSMYTFIIVGIFCTVVGNLLVVLAIAYFKQLQSHTNSFVMSLAVADFLVGLVVMPYSMVRTIEGCWHFGATFCQLHSSLDVMLCTASIFHLSCIAFDRYYAVCNPLVYTFKMSRSRVALLIVVCWAVPLLISFGPILLGLHKLGVDISLPENVCVFLVNRVYAVIASLVAFYLPMVSMLVAYWKIYKAAKRQAMQISAMEAQMAAGVGKDSSKKQKHRNSMRRERKAAQTLGIIMGVFLIFWLPFFTVNIVDPFIEYTTTGVIWDVFLWLGYVNSSLNPFLYGFFNRSFRRAFLLIMGCRICLPGSASGMDLSHSRKDANEQTFFPPELYITKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DFY3</accession>
    <name>A0A3B4DFY3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DFY3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034513.2">
        <property type="protein sequence ID" value="ENSPNAP00000022358.2"/>
        <property type="gene ID" value="ENSPNAG00000031656.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153212"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000194">
        <property type="entry name" value="UDP glucuronosyltransferase 2 family polypeptide a4 short isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF162">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 2 FAMILY, POLYPEPTIDE A1 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043994390" evidence="4">
        <location>
            <begin position="23"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022358.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="257" mass="29813" checksum="3B30D0763A6F91E1" modified="2024-11-27" version="2">MTYRHFLHLVALLLIGSEVTFSGNILVLPGEFSHWYNMRVIVDELITRNHSVTVLIHSASSSISYMQKENFKYTVFKINMEKTHTEIMWQNFINAWMNETVTGIKTLSLFWNILSEFEEYFGAACEGMLLNQELIASLRESHFDVLLFDPMMPCSDLLAENLGLPQVLSIRFTLAYSLERLCGQLPTPPSYVPAVASQGHLTDHMNFIQRVENVLLYVAHTAVFKRQIMLMFDKYYTKITDTFIQSDLQIMRYIEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DHJ7</accession>
    <name>A0A3B4DHJ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CFAP100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017553264.1">
        <property type="nucleotide sequence ID" value="XM_017697775.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017553265.1">
        <property type="nucleotide sequence ID" value="XM_017697776.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DHJ7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022576"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034779.2">
        <property type="protein sequence ID" value="ENSPNAP00000022576.1"/>
        <property type="gene ID" value="ENSPNAG00000029969.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427550"/>
    <dbReference type="CTD" id="348807"/>
    <dbReference type="GeneTree" id="ENSGT00940000153110"/>
    <dbReference type="OMA" id="AWKLSMT"/>
    <dbReference type="OrthoDB" id="10264063at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF3">
        <property type="entry name" value="CILIA AND FLAGELLA ASSOCIATED PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="4">
        <location>
            <begin position="126"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="526"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="351"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="566" mass="66597" checksum="F1A8B634FAF0B472" modified="2018-12-05" version="1">MLSSVSSVQKDSRDGTRTDPNPFKMPEDNSIFLMRNAEKAWRKQEYQKQLCLPVHEKKTYTGRMRARQTELRMKLSEGLEEEEEEEEEESNADHTHTTVQLLQDTPACKVIMIKDRNIEKESIREFIDKKRKMFYLEHALAVKREEIKQLEEVASHEEKKLMKAEKLLEDDTILFDSFLKENDKNSVEAIKIAEQETKAKLEKVAEIKKITAKMVAIKSDISKYEDTLQEYKLYKDFLFKLSPPEWQEKQREKNRRSPKTTSANEKRESEVKSRETDRGKKTPQSDRCREKRSSVGSRELPPREGRATSRHSVKSTPQSGKISSISDTDSSEYEEDPELYFTEPQQLLDLLSELEEQNLSLIQNLQETEEGLEEFRQTMEQTRKNMDHEADQLKRQIDVMTETIQREKERTTELELKARLFNFGKYKAEEDDTLNALSRKVEEVYRSCVGDSEANLSTLQMLTAIEGRLGELLENVEMIPSDRLAIAEKAKEKERRLRLRDEKIYLQKQHQEERMKKAMERAQAEIKKTTGRKLMPRSQPPVRKLKNNQHDDIADKEKEEHMYFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJ89</accession>
    <name>A0A3B4DJ89_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546372.1">
        <property type="nucleotide sequence ID" value="XM_017690883.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJ89"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024447"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010076.2">
        <property type="protein sequence ID" value="ENSPNAP00000024447.1"/>
        <property type="gene ID" value="ENSPNAG00000008991.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423517"/>
    <dbReference type="CTD" id="402848"/>
    <dbReference type="GeneTree" id="ENSGT00940000155490"/>
    <dbReference type="OMA" id="SMLPLNC"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF48">
        <property type="entry name" value="PROTEIN VAPYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="312"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="354"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="384"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="535"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="617" mass="67541" checksum="E337F90C3EDA589C" modified="2018-12-05" version="1">MTRFSYTEYFSLFHSSGMKSSSALAVLSQCFDSGGRRGNLGDVTSISPEDVEPMIQAIRKGDVQTVSELAASTSCIQTQMEGWTALHEAAYWGQAGCVKALLKAKPTSVDKRTLQEQTALLIATDGKHLDCVKCLLEAGADPDISNKNKETPLYKACEQESIDIVRVILAFGGAVNQRCHRGWTALHEAVRRGNTELCETLLQAGAAIDPPSTYGITPLIEAAQHGRTKVVDYLIQKGAKVNLQSCEGTTALSEASKQGHLATVELLLRHHADANKASNTGLLPLHIAAQHGHKEIVSLLLPVTGRAKIRQSGITPLHLAAEFNHENVVSFLISSGSDVNSRLSNQRSSLFHDHRTTALYCAVAAGNAGVVDTLLKAGANPNLDPLSPLMVAVRQGCFRTISTLVEYGADVNARIPAHPTDFPGVLLCTHHLGILQYLLDNGCQAHNCFRCYHKETRQPYITSQSRRGDAGQRIHTMMENTSIPSRTTCTESATRNLQFCEWISSASVCHMAAPLISLLLDYVGNVHLCCRVTEQLNRKEEWIGIKEKALSPRPLMHLCRLKIREQVGAHRLKSLNTLPLPGRLLHYLRCSQTSTREYPDLMLGTAQTQHAGPQRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DK34</accession>
    <name>A0A3B4DK34_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DK34"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023294"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013011.2">
        <property type="protein sequence ID" value="ENSPNAP00000023294.1"/>
        <property type="gene ID" value="ENSPNAG00000007836.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166182"/>
    <dbReference type="OMA" id="MYGTHAS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF4">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="378" mass="37150" checksum="9034BF51B756271E" modified="2018-12-05" version="1">MVFPRYDHGFISADQPTFQRRMLKGDQGQAGPPGPPGPPGAPGPRGPPGNTGKDGPRGPPGEQGAPGRDGMEGPRGLPGDPGEDGQPGYAGPQGPPGLKGEQGEPGFKGDRGMDGFPGLKGDKGDVGESGPQGEMGPPGEKGATGSSDVIAFNDKLLDAFQGPPGPPGPPGPPGKDGSKGELGLPGPAGLDGEKGQKGEIGDPGDPGERGEKGEMGLSGPAGMDGPKGEKGDSRLEDNLVQMISQPGPPGPPGPRGPPGFMGHHGMPGSKGDPGESIKGEKGDSGASGIQGPHGPQGPPGPAGLVGLPGSKGEKGKQGEPGLDGFPGLVGEKGDTGEKGEKGDRGSVGKRGLKGQKGEQGPPGLDQPCPVVRGPELLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKB3</accession>
    <name>A0A3B4DKB3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tripartite motif-containing protein 2</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">E3 ubiquitin-protein ligase TRIM2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase TRIM2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Forms homooligomers. Interacts with TRIM3; this interaction reduces TRIM2 activity. Interacts with myosin V; myosin V may not be a substrate for ubiquitination. Interacts with NEFL. Interacts with phosphorylated BCL2L11. Interacts with SIRPA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKB3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001796"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011519.2">
        <property type="protein sequence ID" value="ENSPNAP00000023898.2"/>
        <property type="gene ID" value="ENSPNAG00000008378.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155905"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19824">
        <property type="entry name" value="Bbox2_TRIM2_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20482">
        <property type="entry name" value="CC_brat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14960">
        <property type="entry name" value="NHL_TRIM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16767">
        <property type="entry name" value="RING-HC_TRIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000007">
        <property type="entry name" value="Putative tripartite motif-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000004">
        <property type="entry name" value="Tripartite motif containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000032">
        <property type="entry name" value="Tripartite motif containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000154">
        <property type="entry name" value="Tripartite motif-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057750">
        <property type="entry name" value="TRIM2/3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF58">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="23">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="18">
        <location>
            <begin position="348"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="503"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="550"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="594"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="639"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="686"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="730"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="453"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="183"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023898.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="774" mass="84086" checksum="7241EB67662F3135" modified="2024-11-27" version="1">MLNAEVKFPRSPSLSLSFSLSVQASFRMASESSTIPSPVVRQIDKQFLICSICLDRYDNPKVLPCLHTFCERCLQNYIPAHSLTLSCPVCRQTSILPEKGVAALQSNFFITNLMEVLKRSPDSGRGDEHAGLDSIDAVATGQPLSCPNHGGNVMEFYCPPCETAMCEECTSGEHAEHATVPLKDVLEQHKASLQEQLDAVKKRLPEIDSALQVLSEVLQQLTNQKSSIEEDIHATFDELQKTLNVRKSVLLMELEVNYGLKQKVLQTQLASLLQGQDGMRSSCSFTEQALSHGSEAEVLLVKKQMSERLGELANQELPLRPEENGQLEFLAETEGLRKSIHNLGAIVTTNAIAGETVATGEGLRHCTAGRPTSITITTKDKDGGLCRTGGAQLTAELIGAEDGAPAGEGELVDHKNGTYEFPYTLPHEGRFTLSLKLYGHHIRGSPFNIRATKAADPSANADGAKKRLKSPGSGHVKQRAIKRPASMYSSGRRKENPIEDDLMFRIGTKGRNKGEFTNLQGVAASNFGRVLIADSNNQCVQIFSNDGQFKSRFGVRGRSPGQLQRPTGVAVHPNGDIIIADYDNKWVSIFSSDGKFKTKIGSGKLMGPKGVSVDRNGHIIVVDNKACCVFIFQPNGKLVTKFGNRGNGDKQFAGPHFAAVNQNNEIILTDFHNHSVKVFNPEGEFLLKFGSNGEGNGQFNAPTGVAVDVNGNIIVADWGNSRIQVFDGSGSFLSYINTSADPLYGPQGLALTSDGHVVVADSGNHCFKVYRYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DNK5</accession>
    <name>A0A3B4DNK5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA binding protein fox-1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">RNA-binding protein that regulates alternative splicing events.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DNK5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025962"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006305.2">
        <property type="protein sequence ID" value="ENSPNAP00000025962.2"/>
        <property type="gene ID" value="ENSPNAG00000001620.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166165"/>
    <dbReference type="OMA" id="KPQTPLM"/>
    <dbReference type="OrthoDB" id="5382468at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055013">
        <property type="term" value="P:cardiac muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003015">
        <property type="term" value="P:heart process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12407">
        <property type="entry name" value="RRM_FOX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000004">
        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025670">
        <property type="entry name" value="Fox-1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034237">
        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017325">
        <property type="entry name" value="RBFOX1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF40">
        <property type="entry name" value="RNA BINDING PROTEIN FOX-1 HOMOLOG 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12414">
        <property type="entry name" value="Fox-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037932">
        <property type="entry name" value="Ataxin_2_bd_A2BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="148"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037932-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025962.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="389" mass="41205" checksum="97FE949B9E0810DB" modified="2024-11-27" version="2">MLSSPTVILQPYGLPVYPQTASCYPSLVQGAPAQEAGPGSADPTLPQVYAPPPSYPPPGQAPPTPAGRLPPLDFNSAHPSSEYPDHHQLRVYQSTQHEGVEPITTNNTDDALAPVTSDPQPLSASVASCGGGAGSGGEEEGSGKAQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENAVEADRAREKLNGTIVEGRKIEVNNATARVVTKKPQTPLVNAAGWKINPVMGAMYAPELYTVASFPYPVPTPALAYRGSALRGRGRAVYNTIRSAATAATPAAVPAYPGVVYQDGLYGTEVYGGYPATYRVAQSPSAAATAAYSDGYGRVYATATDPYHHSVGPTTTYGVGTMVRDPVVSYSVTLLNQNTFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQH8</accession>
    <name>A0A3B4DQH8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ferrochelatase</fullName>
            <ecNumber evidence="14">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FECH</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:22586"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017569206.1">
        <property type="nucleotide sequence ID" value="XM_017713717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQH8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025356"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007813.2">
        <property type="protein sequence ID" value="ENSPNAP00000025356.1"/>
        <property type="gene ID" value="ENSPNAG00000010385.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436946"/>
    <dbReference type="CTD" id="2235"/>
    <dbReference type="GeneTree" id="ENSGT00390000016258"/>
    <dbReference type="OMA" id="DPYHCEC"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000003">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9 14" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="409" mass="46244" checksum="CA2A429468B9999D" modified="2018-12-05" version="1">MALLGVACRFLRFSRCGGQVSLCVRAPLRWQSTATAAAFASPPTPEAQENRKPKTGILMLNMGGPEKLEDVHDFLLRLFMDTDLMQFPVQNKLGPFIAKRRTPKIQEQYSKIGGGSPIKAWTTMQGEGMVKLLDEMCPETAPHKFYIGFRYVNPLTEEAIEEMEKDGVERAVAFTQYPQYSCSTTGSSLNAIYRYYSNRATRPKMRWSVIDRWPTHPLLIECFAEHVRNELDKFPAEKRDDVVILFSAHSLPMSVVNRGDPYPQEVGATVQRVMERLGHCNPYRLVWQSKVGPMAWLGPQTDEVIKGLCQRGKKNLLLVPIAFTSDHIETLHELDIEYSQILGEECGVENIRRSESLNGNPLFFKALADLVRSHLKSNEPCSRQLTLRCPLCVNPTCGQTKAFFASQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DT68</accession>
    <name>A0A3B4DT68_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lysosomal-associated transmembrane protein 4B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LAPTM4B</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Required for optimal lysosomal function. Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation. Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation. Plays a role as negative regulator of TGFB1 production in regulatory T cells. Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homooligomer; upon reaching the lysosomes. Interacts with MCOLN1. Interacts with NEDD4; may play a role in the lysosomal sorting of LAPTM4B; enhances HGS association with NEDD4; mediates inhibition of EGFR degradation. Interacts with PIP5K1C; promotes SNX5 association with LAPTM4B; kinase activity of PIP5K1C is required; interaction is regulated by phosphatidylinositol 4,5-bisphosphate generated by PIP5K1C. Interacts with HGS; promotes HGS ubiquitination. Interacts with SNX5. Interacts with SLC3A2 and SLC7A5; recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation. Interacts with LRRC32; decreases TGFB1 production in regulatory T cells. Interacts with BECN1; competes with EGFR for LAPTM4B binding; regulates EGFR activity. Interacts with EGFR; positively correlates with EGFR activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endosome</location>
            <location evidence="5">Multivesicular body lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LAPTM4/LAPTM5 transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548948.1">
        <property type="nucleotide sequence ID" value="XM_017693459.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DT68"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026296"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005505.2">
        <property type="protein sequence ID" value="ENSPNAP00000026296.1"/>
        <property type="gene ID" value="ENSPNAG00000001815.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425070"/>
    <dbReference type="CTD" id="55353"/>
    <dbReference type="GeneTree" id="ENSGT00940000153446"/>
    <dbReference type="OMA" id="LTDPGQY"/>
    <dbReference type="OrthoDB" id="10002163at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004687">
        <property type="entry name" value="LAPTM4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051115">
        <property type="entry name" value="LAPTM_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12479">
        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12479:SF6">
        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03821">
        <property type="entry name" value="Mtp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="17" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00010076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="225" mass="25247" checksum="0753C8806DD1BAC1" modified="2018-12-05" version="1">MISPWDRWYTTSCCLCCHVRTGTIILGIWYMLINAVVLLILLSALNDPVQYHYHLTSAELGTDLDVMDDANMCIAAAISLLMILICGMATYGAYKQHAAWIIPFFCYQIFDFALNTLVAISVVVYPNTIQDYLQQLPGTFPYKEDLMSTSNMCLVFAVLLFIGCILAFKAYLIACVWNCYRYVSGRGTTEVLVYVTTNDTTVLLPPYEEVVAIPPKEPPPQYVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTL2</accession>
    <name>A0A3B4DTL2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sodium channel modifier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SCNM1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017577192.1">
        <property type="nucleotide sequence ID" value="XM_017721703.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTL2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026790"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004249.2">
        <property type="protein sequence ID" value="ENSPNAP00000026790.1"/>
        <property type="gene ID" value="ENSPNAG00000012139.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441929"/>
    <dbReference type="CTD" id="79005"/>
    <dbReference type="GeneTree" id="ENSGT00390000010811"/>
    <dbReference type="OMA" id="NGKYACT"/>
    <dbReference type="OrthoDB" id="1924550at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033570">
        <property type="entry name" value="SCNM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031625">
        <property type="entry name" value="SCNM1_acidic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031622">
        <property type="entry name" value="Znf-SCNM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32297">
        <property type="entry name" value="SODIUM CHANNEL MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32297:SF1">
        <property type="entry name" value="SODIUM CHANNEL MODIFIER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15805">
        <property type="entry name" value="SCNM1_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15803">
        <property type="entry name" value="zf-SCNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Sodium channel modifier 1 zinc-finger" evidence="12">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium channel modifier 1 acidic C-terminal" evidence="13">
        <location>
            <begin position="211"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="168"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="231"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF15805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="261" mass="29851" checksum="B0CC53EA9283FAD9" modified="2018-12-05" version="1">MSFKREGDDQSQLNVLKKRRVADLLSNFIPEDEAVLMKNGRYSCLVCSHRPVFDTVDMLAIHRKGKRHLEGLKWFYGRKARLQNETDKKRHQEYVNKEEDQQEAGRSAPLLTETRKITHHALLRSAPYSSCHKKASERSESVTMGFSPMVSADSVSQSYKATAKNNIHTETKPAYAETNPNQGQNGKQMKKKDAKKDSKAVSLSESEPWTEQRRSELEHYLKLKSAGWLQDPSGKWVKDENVEFDSDEEEPLPLLPSSETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DW66</accession>
    <name>A0A3B4DW66_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Replication factor C subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Activator 1 subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RFC4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA. The RFC4 subunit probably functions as a scaffold on which the other complex components can assemble.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Subunit of the RFC complex, an heteropentameric complex consisting of a large subunit RFC1 and four small subunits RFC2, RFC3, RFC4 and RFC5; the RFC complex interacts with PCNA. Forms an heterotetrameric complex with RFC2, RFC3 and RFC5; this complex has ATPase activity but is not stimulated by PCNA. The heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 interacts with RAD17. Interacts with ATAD5. Interacts with CTF18. Interacts with CNTD1; this interaction facilitates crossover formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DW66"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027219"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003144.2">
        <property type="protein sequence ID" value="ENSPNAP00000027219.2"/>
        <property type="gene ID" value="ENSPNAG00000012677.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074917"/>
    <dbReference type="OMA" id="GCQSGSF"/>
    <dbReference type="OrthoDB" id="10249205at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000032">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000010">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="64"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027219.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="358" mass="39682" checksum="26D447DDBDEB2BD6" modified="2024-11-27" version="2">MQAFLKGSTQSVKPQRDKAAAGSGGEKRQKAVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELYGPELYRQRVLELNASDERGIQVVREKVKNFAQLTVAGTRPDGKACPPFKIIILDEADSMTSAAQAALRRTMEKESRTTRFCLICNYVSRIIEPLTSRCSKFRFKPLANDIQQERLIEICEKEIVLYILLQGIEALVKVSEGDLRKAITLLQSAARLSTEKEITESVIIEIAGVVPPKVIENLLQTCYKGTFEKLEVAVKTMIDEGYAGTQILNQLHDIIIEESLSDKQKSVITEKMAVVDKCLVDGADEYLQLLGLSSVIMQQATQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXQ4</accession>
    <name>A0A3B4DXQ4_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heat shock factor binding protein 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HSBP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXQ4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029182"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016051.2">
        <property type="protein sequence ID" value="ENSPNAP00000029182.2"/>
        <property type="gene ID" value="ENSPNAG00000015119.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006293"/>
    <dbReference type="OMA" id="MERANMD"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009643">
        <property type="entry name" value="HS1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424:SF0">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06825">
        <property type="entry name" value="HSBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029182.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="70" mass="7936" checksum="CA59B77FC07DB3AF" modified="2024-11-27" version="2">GGSSDPKHPHTLREVQTLLQQMQDKFQTIFSLIGTLDEMSTRIDDLEKNISDLMTQAGVEEFEGENKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DYJ9</accession>
    <name>A0A3B4DYJ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580212.1">
        <property type="nucleotide sequence ID" value="XM_017724723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DYJ9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035593.2">
        <property type="protein sequence ID" value="ENSPNAP00000029482.2"/>
        <property type="gene ID" value="ENSPNAG00000030344.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108443848"/>
    <dbReference type="GeneTree" id="ENSGT00940000163149"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF62">
        <property type="entry name" value="CRYGM2B PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="3"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="132"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029482.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="176" mass="21693" checksum="30D541F52FAE5618" modified="2024-11-27" version="2">MMGKIIFYEDKNFMGRSWECSSDCSDMSSYISRCHSCRVESGCWMVYDRPNYMGNQYFIRRGDYADYMSMWGMNSWIKSCRMIPMYSGPYRMRVYERENYMGQMMEMTDDCESFMDRYHWSHGCMSCHVMDGHWLMYDQPNYRGKMWYFGPGHYRSFRDWGNMRFMSMRRIMDSWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E058</accession>
    <name>A0A3B4E058_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hepatocyte growth factor-regulated tyrosine kinase substrate</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HGS</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
            <location evidence="1">Multivesicular body membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037394844.1">
        <property type="nucleotide sequence ID" value="XM_037538947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E058"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029065"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015830.2">
        <property type="protein sequence ID" value="ENSPNAP00000029065.1"/>
        <property type="gene ID" value="ENSPNAG00000003331.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429678"/>
    <dbReference type="GeneTree" id="ENSGT00940000158297"/>
    <dbReference type="OMA" id="DQQCSAK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15720">
        <property type="entry name" value="FYVE_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21387">
        <property type="entry name" value="GAT_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03569">
        <property type="entry name" value="VHS_Hrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1940:FF:000003">
        <property type="entry name" value="Hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000014">
        <property type="entry name" value="Hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000028">
        <property type="entry name" value="Putative hepatocyte growth factor-regulated tyrosine kinase substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017073">
        <property type="entry name" value="HGS/VPS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024641">
        <property type="entry name" value="HRS_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46275">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR-REGULATED TYROSINE KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46275:SF1">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR-REGULATED TYROSINE KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12210">
        <property type="entry name" value="Hrs_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036956">
        <property type="entry name" value="Hrs_Vps27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VHS" evidence="14">
        <location>
            <begin position="15"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="13">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="279"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="707"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="463"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="304"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="718"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="739"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="763" mass="85257" checksum="3478539B30CD1453" modified="2018-12-05" version="1">MGKGGGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAIGAIKKKLNDKNPHVALYALEVLESVVKNCGQTVHDEVASKTTMEELKDLLKQTEPNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVMTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCFELLNNHPSLSPPPRKAEGKSSSGGQAELPPEYLTSPLAQQSQMPPKRDEAALQEEEELQLAIALSQSEAEEKERMRQKNSYSVYPKADPTPVTSSAPPVSTLYSSPVNSSAPSAEDVDPELARYLNRTYWEKKQEEVRKSPTPSAPAPVPLAEPLPISQPVESHAPVQPINIVEQQYQNGESEENHEQFLKALQNAVTTFLNRMKSNHMRGRSITNDSAVLSLFQSINNMHPQLLDILNQLDEKRLYYEGLQDKLAQVRDARAALNALRDEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEMQRQLAIQRLQEQEKERQLRLEQQKHTIQMRAQMPAFSLPYAQMQSLPPNVAGGVVYPPAGPPSYPGTFSPAGSVEGSPMHGVYMNQPGQTGAGPYQAMPVSATDPNMVNAYMYQTAGTGGQPAAPGQAPPTTNPAYTNYQPTPTQGYQNVVSQAQSMPPMSQAAPTNGMAYMGYQPYNMQSMMSALPGQDPNMPPQQQYMPGQQPMYQQMPPPGGPQQQAQQQPQQAPPGSAEAQLISFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0W2</accession>
    <name>A0A3B4E0W2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S100P-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017550863.1">
        <property type="nucleotide sequence ID" value="XM_017695374.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0W2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029350"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035489.2">
        <property type="protein sequence ID" value="ENSPNAP00000029350.1"/>
        <property type="gene ID" value="ENSPNAG00000015356.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426109"/>
    <dbReference type="GeneTree" id="ENSGT01140000282706"/>
    <dbReference type="OMA" id="DEWQNLN"/>
    <dbReference type="OrthoDB" id="8945510at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026097">
        <property type="entry name" value="S100PBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14455">
        <property type="entry name" value="ASKOPOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14455:SF0">
        <property type="entry name" value="S100P-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15427">
        <property type="entry name" value="S100PBPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="585" mass="64423" checksum="85B8A7030A62D936" modified="2018-12-05" version="1">MADQKIPLKTIFHSRGSDKTEFAHLRPLSAYSRMITCETTDTSSSDLSSIPNSPFCNIKVEIINSHAFNLKRRLDDSCLDETYETPLKKKPCLATCHSPDLACVLDSVLPDPKNITPSSSLVAEKVNNAENSSSNEETDPQVTSLKSSSLVWPESSDESDGQSSPSLSLSNDRSSSPPILVSGDMGDLGPAFDFDIDEIMCLSPIDRYDEQPSSDGLDDFVQSCQTFCEEQRSCKHSEACEGHGSSTKGQTSEQVQGVMDMGVESDEGYFTKSYKIAKLKGSKDVSPPESQTVTPVMSTPLDKLRQLVKRSPVLKKLWKTGQGSPVVNQEPLGASSPITLYGSVSLDASNTESFPETLFLDHNENKEGEVGTSIQKFLGCHAQQAPVVIVVDSHAEKMKDVPSASQKVDPKFQVTFQEEADSSTSVESPSPLQVKVKSKVVLPSIQIPQVKTKPAVKVAAEAAATEQPEEKPQSGDTKLVVFYEEEDWEREKTVYVDSVIRHMKDNTGAGDGVMTELLNLMNTVANQGNGSYGRPWQHPSDLTHRNYQPRHSGHLFSLDEWQNLNYRYHRRFAKVPQPFIRSPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2D4</accession>
    <name>A0A3B4E2D4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 223</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017553997.1">
        <property type="nucleotide sequence ID" value="XM_017698508.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2D4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029853"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017351.2">
        <property type="protein sequence ID" value="ENSPNAP00000029853.1"/>
        <property type="gene ID" value="ENSPNAG00000016213.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427920"/>
    <dbReference type="CTD" id="79064"/>
    <dbReference type="GeneTree" id="ENSGT00390000012589"/>
    <dbReference type="OMA" id="PLEHISC"/>
    <dbReference type="OrthoDB" id="5950063at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026100">
        <property type="entry name" value="Tmem223"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14549">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14549:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="30178" checksum="69E9BAFA9CD64D39" modified="2018-12-05" version="1">MGFQGVLLAARICRSSCAGLLFPHRLTSVQSLRAFTAVSKRSVQVTDQTSVFTPSRWFSPLSPSPRGRAHGLVSQQLGGRSSHSFTSTAVVKDVLLFEHDRTRFFRVLALFCGGQFLFWTYLAYFAFTGLRDTRKSRKEPQRVRTDLGLFSFDMNLGSNAWRFGFTLGCLVIGGGIVGLGVLFSRRSVSRVVLHKGGGKVTVHTQSPLGANKAWHLTVPLNQVACHAHRQESPSFIPLKVKDHKFYFLMDKEGTLNNPRLFDITVGAYRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2G1</accession>
    <name>A0A3B4E2G1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin tyrosine ligase-like family, member 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamyl-[protein] + glycine + ATP = glycyl-L-glutamyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67180"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:17207"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:167890"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67181"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2G1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029419"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016506.2">
        <property type="protein sequence ID" value="ENSPNAP00000029419.2"/>
        <property type="gene ID" value="ENSPNAG00000015497.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154857"/>
    <dbReference type="OMA" id="SHHMGRL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070736">
        <property type="term" value="F:protein-glycine ligase activity, initiating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000032">
        <property type="entry name" value="tubulin monoglycylase TTLL3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051437">
        <property type="entry name" value="TTLL_monoglycylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870:SF2">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="133"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029419.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="822" mass="94442" checksum="384FD679FD4495D5" modified="2024-11-27" version="2">MPVLTRARKMQLVSIEKKVDVDVKSDLEHSDMQHHMPGITLQHTALDREVCYQMENNWVCVCEAVPPVEGRVRCSRVSLPVLKLDRLRMAKALVDKAVKERKVFSIEGPYPVIRAALRARGWVERQLPRRPLGCCHQGDQEAEGTEETDSSDDDGADRGQDGDREDDTDEMTDLMSRLVRNETTYFYWTTRRDTVDCLSLRKDQMTNHYAKAGSFTTKVGLCMSLRNLQWFDSTDPDTFFPRCYRLGAEDEKHAFTEDFRRTACTSLLRYVVERAEGGEVDTTGEFCNVKHSQGMDKQHKLRSNQRLGARLIDTALHVCQEYLSSQEHNDIDVDCPSSLSEQQWDDFIQKYYLVIHDGAIIEGCSEYAERCRSMLTRMQKVCPQLQTDGLNNIWIIKPGALSRGRGIVCMNRLDEMLSLVDSEPALIKDSKWVVQKYLERPLLVHGTKFDLRQWFLVTDWNPLTVWFYDECYLRFSTQPYSTHRLDSSVHLCNNSIQKHFRPSAGRDPSLPAENMWSCAEFRSWLASSGRGRLWEEVVLPGMRLAVIQTLLTAQDSVEPRKASFELYGADFILGRDLHPWLLEVNVSPTMASSTAITARLCPAVQEDTLRVVLDRRYDRNAHTGGFQLIYKQAAVEVPQYIGVNLLVEGAQIRRHRASPCKTFIRSHCEPLHKSRPALPVSECSSDKENQSNEVKRTWPNVSRKFTIERSSTCQPLLEKRRRRRALPSASCPLPNLSDRHHLQTSYARLHAYTELAHTRPHRTRRNVLSLNHLSPSLEIISIRPELKYSQSTHAPTRTAHVSHPTLRMHRYLSPINTQTPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E4V9</accession>
    <name>A0A3B4E4V9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Voltage-dependent L-type calcium channel subunit beta-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Calcium channel voltage-dependent subunit beta 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Increases CACNA1B peak calcium current and shifts the voltage dependencies of channel activation and inactivation. Increases CACNA1C peak calcium current and shifts the voltage dependencies of channel activation and inactivation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1C) and the ancillary subunits CACNB3 and CACNA2D1. The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio. Interacts with CACNA2D4. Interacts with FASLG. Interacts with CBARP; prevents the interaction of CACNB3 with the alpha subunit CACNA1C thereby negatively regulating the activity of the corresponding calcium channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537228.1">
        <property type="nucleotide sequence ID" value="XM_017681739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E4V9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031647"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020819.2">
        <property type="protein sequence ID" value="ENSPNAP00000031647.1"/>
        <property type="gene ID" value="ENSPNAG00000019154.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410585"/>
    <dbReference type="CTD" id="565158"/>
    <dbReference type="GeneTree" id="ENSGT00950000182837"/>
    <dbReference type="OrthoDB" id="5962384at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000023">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000049">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046937">
        <property type="entry name" value="CAB1-4_N_A-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008079">
        <property type="entry name" value="VDCC_L_b3su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000584">
        <property type="entry name" value="VDCC_L_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11824">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01696">
        <property type="entry name" value="LCACHANNELB3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="Guanylate kinase/L-type calcium channel beta subunit" evidence="17">
        <location>
            <begin position="222"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="438"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="529"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="546"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="561" mass="63131" checksum="E92B8403FA1E5F25" modified="2018-12-05" version="1">MVQVKMSQSAADPLPGAESGSQSHYFQLPRKLPKRKSRFKRSDGSTSSDTTSSFIKRQGSADSYTSRPSDSDLSAEEDREAYRREAERQAQLQLDRAKSKPVAFAVRTNVNYCGALDEDCPVQGAAINFEAKDFLHIKEKYNNDWWIGRLVKEGADISFIPSPLRLEAMRLKQEQKQRKSGGNSSSSLGDMVSGGWRPTPPSAAKQKQKQAEHIPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFEGRISITRVTADLSLAKRSVLNKRPIMERSNTRSSLAEVQSEIERIFELAKTLQLVVLDADTINHPAQLAKTSLAPIVVYVKVSSPKVLQRLIKSRGKSQSKHLNVQMMAGDKLAQCPPEMFDVILDENQLEDACEHLAEYLEVYWRATHLPGTAPLNPLLEQNLMSLPSAISSLQNQNQQLLSAGGAGGVEEEEQGEQDSLMPSDEASDSRSLRRSTSRSLHPPEDDEEEEEEEEECEEEEEEYEPPRHSHTYRDAYPPNRNHPGGVHSANGHESQDRLLQQRDSQDGHNHRGNRQRPRAWPRDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EAL4</accession>
    <name>A0A3B4EAL4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Muscarinic acetylcholine receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Postsynaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EAL4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032276"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037245.2">
        <property type="protein sequence ID" value="ENSPNAP00000032276.2"/>
        <property type="gene ID" value="ENSPNAG00000020131.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430663"/>
    <dbReference type="CTD" id="553978"/>
    <dbReference type="GeneTree" id="ENSGT00940000158450"/>
    <dbReference type="OMA" id="SFRNQAC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000047">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000522">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000995">
        <property type="entry name" value="Musac_Ach_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF209">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR M5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00243">
        <property type="entry name" value="MUSCARINICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0770">Synapse</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="48"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032276.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="545" mass="61454" checksum="DE55DBB092782E6E" modified="2024-11-27" version="2">MGAMDRGLTDNSSISANASDIHLVTHSLWEVITIATVSAIVSLITIIGNVLVMLSFKVNSQLKTVNNYYLLSLAFADLIIGIFSMNLYTSYILMGYWSLGNLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWFVSFVLWAPPILCWQYFVGKRTVPERQCQIQFFSEPVITFGTAIAAFYVPVSVMTILYCRIYKETEKRTKDLAELQGVNHSTDSDSKAQPQRAIIRSCFSSISRDQKQKQSSWASSSRSKAAKSATASNDEWSKADQLATFNSYASSEDEERPVSPGLLPAYRNHACGENKAALSNDGEQLSSHEDENFFPSPIKANSQKNKKCVSYKFKPAAKDSNSLQTRNEDTKAASFSSAESMSVPSTASSSKPIDATLKSQITKRKRMVLIKERKAAQTLSAILLAFILTWTPYNIMVLISTFCSDCIPLSLWHLGYWLCYVNSTVNPMCYALCNKTFQKTFRMLLLCQWKKKREDEKLYCNCFLTVIHPFRLMVLSVFSPWKDGRKNLWIFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECC7</accession>
    <name>A0A3B4ECC7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase family A palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">POLN</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ECC7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032886"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037599.2">
        <property type="protein sequence ID" value="ENSPNAP00000032886.2"/>
        <property type="gene ID" value="ENSPNAG00000021241.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159015"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08638">
        <property type="entry name" value="DNA_pol_A_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000002">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.10">
        <property type="entry name" value="Taq DNA Polymerase, Chain T, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040940">
        <property type="entry name" value="DNA_pol_P_Exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF27">
        <property type="entry name" value="DNA POLYMERASE NU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18049">
        <property type="entry name" value="DNA_pol_P_Exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="3">
        <location>
            <begin position="703"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="549"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032886.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="910" mass="103358" checksum="763F56968FC6489A" modified="2024-11-27" version="2">MESPSCSTLTAGPLSLAAQTVMAALRAQYAGRIHRTRHSELGTAEKGRRFYNHSPEMELPSQPAISLFQSKDNTFSEWETDEPGSLKLRDYGLDGSLLALNDNKITVSGNHILKIHTQSEEPFPMKDFSDWEDPQAVPDAIVVPEKQREISRTKKNIRLMCLSEDVFKAERVTRAPPRKRWSFSHLQMPAMSCSKDFIEEQKSDFGRQNREGSSKKMEADCFEGKMETVEGVMTMPKQRKDQEVGMSEKIQLNGLWSEGPDKESVVYTKTTNHSYISEFPKQKRSASEIDNQIHPEHPEGVKERYAISASSRLTLALPSDARVCDTSRLSQEERVCVLEEASRARALVVSMVYQDGTTQLDPEQKPLPAVCGLLVLLKKSLESLDLDQTGAAKERVLLLRLEQRPVWVQQDSQHNQDLFTREILLQMVCGMQSLVCYKSKDLLRTILRHFSRDLSWKQVAACQVLDPQIAAWLLDPADSAHCFQDLLSKHCSLPATHTPAQPALGHSKVRTHANTHTLQQHKQEGQKTVTPVLSLSLSAMESHSIHVDKENLKKTSDMLGNKLKQLEQEAHQAAGQQFLVSSSAQLRLVLFEKLRLHERCENKKLPKTVFKQQQSTSEAALLQLQDLHPLPKIILEHRQVKYPLSLSLSQSFISSTWNQTSTVSGRLSAKHPNFQALPKLPVQITKKQYIQGKEEEFVTVHPRSMFIPQEGWTFLSADFCQVELRLLAHLSSDPELLRIFQNPEADVFTMLASQWWGINEDSVSSEDRDHAKRIVYSVVYGAGKKRLSGILRVSLEEASHFQDSFLQTYKEVQNFIQHTVQHCHKYGYVKSIMGRRRFLPHVHSTDWGVRNQAERQAVNFVVLVAQIHDELLFEVEDSQVEDFAVLVKEIMESLEHIDCLGVSLNVTSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EDA7</accession>
    <name>A0A3B4EDA7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA polymerase epsilon subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA polymerase II subunit 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">POLE3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Accessory component of the DNA polymerase epsilon complex. Participates in DNA repair and in chromosomal DNA replication. Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit POLE and the accessory subunits POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Heterodimer with CHRAC1; binds to DNA. Component of the CHRAC ISWI chromatin remodeling complex at least composed of SMARCA5/SNF2H, BAZ1A/ACF1, CHRAC1 and POLE3; the complex preferentially binds DNA through the CHRAC1-POLE3 heterodimer and possesses ATP-dependent nucleosome-remodeling activity. Within the complex, the heterodimer with CHRAC1 interacts with SMARCA5/SNF2H; the interaction is direct and enhances nucleosome sliding activity by the SMARCA5/SNF2H and BAZ1A/ACF1 interaction. Within the complex, the heterodimer with CHRAC1 interacts with BAZ1A/ACF1; the interactions are direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017552522.1">
        <property type="nucleotide sequence ID" value="XM_017697033.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EDA7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034512"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026407.2">
        <property type="protein sequence ID" value="ENSPNAP00000034512.2"/>
        <property type="gene ID" value="ENSPNAG00000023860.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427085"/>
    <dbReference type="CTD" id="54107"/>
    <dbReference type="GeneTree" id="ENSGT00940000164386"/>
    <dbReference type="OMA" id="KQNHRTI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22928">
        <property type="entry name" value="HFD_POLE3_DPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000041">
        <property type="entry name" value="DNA polymerase epsilon subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051377">
        <property type="entry name" value="DNA_Pol-Epsilon_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46172">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46172:SF1">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="12">
        <location>
            <begin position="9"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="89"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034512.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="148" mass="16741" checksum="990ABDC412E4AAA5" modified="2024-11-27" version="2">MAERPEDLNLPNAVITRIIKEALPDGVNVSKEARRAISQAASVFVLYATSCANSFAMKAKRKTLNAGDVMSAMEEMEFERFLPPLREALEAYKKGQKGKKEASEQKRKDKEKKNGTENDKSREEEEDEEEHMEDDDGDNEGEEDDVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EE55</accession>
    <name>A0A3B4EE55_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Adhesion G protein-coupled receptor G3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ADGRG3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EE55"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033579"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024589.2">
        <property type="protein sequence ID" value="ENSPNAP00000033579.1"/>
        <property type="gene ID" value="ENSPNAG00000022293.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166567"/>
    <dbReference type="OMA" id="TLFKGPQ"/>
    <dbReference type="OrthoDB" id="6134459at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF285">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017181583" description="Adhesion G protein-coupled receptor G3" evidence="7">
        <location>
            <begin position="21"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="8">
        <location>
            <begin position="68"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
        <location>
            <begin position="222"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="55646" checksum="B2DF56AF8A2243F7" modified="2018-12-05" version="1">MGRCGLFAVFILSSVALTEGDPSSNGSRIFCYKVPRHCQENESNIPRCIEKQMRNCRFSLPRTKNPDFYLQTVNSSYEVTVDTVYGHVIHIPSEAVLKSSGEQLGNGHELHVTVSVLNRSLFEAQSDEGSPHEQVLGVWLGNQDVHDLSHPVRMVFVNVHQNGRGKCVFWKLLTNSEQGSWSTDGCNTIQNNTDFICECSHLSFFAVLISPDVPDPVDVQHLEYISYIGSSLSVVFTVVVIVMYLWHKKPKAGQTLIIHIQLTGSLLLLHLFFLASSVWSSAEWASCWTLGFILHWALLATFTWMAIEGFHLYLLLVRVFNICISRYRLKLSLVGWGVPTATVMICGMAGAYGKYTLMENASRTTSLCWVTTKAARYITVNGYLGLVLLFNAAILGLMVVKMQQLRARKIQAGNRGRRMWKDWASLLGLSCVLGLPWGLAFITHGPQSISLSAIYLFTVLNAFQGFLMFLWFLSITCKSFHEEQRSTKGSSMSNVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EF89</accession>
    <name>A0A3B4EF89_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">IGKV4-1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EF89"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035197"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027799.2">
        <property type="protein sequence ID" value="ENSPNAP00000035197.2"/>
        <property type="gene ID" value="ENSPNAG00000024961.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01080000257344"/>
    <dbReference type="OMA" id="AGHCLAW"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001495">
        <property type="entry name" value="Si:dkey-234i14.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043534358" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="22"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035197.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="151" mass="16416" checksum="4C4B698DD0AB7B0E" modified="2024-11-27" version="2">MTLVSIFIWTLTLWAQGSSGQVTVTQTPSVKIVSPGDSLTISCRISTAVYKDDYLSWYQQKPGEAPKPLIYRATSKQSGVPARFSGSGSGTDFTLTISNVQIEDVGDYYCQSVHRISGSWVITQCLVGVQKPPSVQLHRDCTAAAGLYCRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFV7</accession>
    <name>A0A3B4EFV7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Interleukin-1 receptor type 1-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017579477.1">
        <property type="nucleotide sequence ID" value="XM_017723988.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFV7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034730"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026843.2">
        <property type="protein sequence ID" value="ENSPNAP00000034730.1"/>
        <property type="gene ID" value="ENSPNAG00000024177.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443377"/>
    <dbReference type="CTD" id="8808"/>
    <dbReference type="GeneTree" id="ENSGT01090000259985"/>
    <dbReference type="OMA" id="LRYHMPP"/>
    <dbReference type="OrthoDB" id="9940746at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF26">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017272304" description="Interleukin-1 receptor type 1-like" evidence="10">
        <location>
            <begin position="22"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="114"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="11">
        <location>
            <begin position="370"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="62694" checksum="750043D30C113795" modified="2018-12-05" version="1">MIHTVSFQLMLILILSSQTEGDHKTKQLEITAGHVFPLECKQFLQEDTEVIWSWGQKQNSDEHGVPAGVRLRNQLLWFFPAQPSHSGNYSCNFRRKGRKEETVVNISVANRTCPSVKSFPFTLKKGINQFLDCNFGHISKILNLGADIHVTWMKDCSPLPSKEIILQFLNVKESDRGEYTCLVNFTHEGNWYTAAQTLTVSVVSGSIPQKPTVVKPHQQETHTVKVGIRYELNCRVFVGTGEDDTEPTVVYWLVNGIYSEDIPESIVTGDVNCTSVDNTKYCERKLTILEVQAKFLHIPFMCFITNSKGTSNGTLTLVPASQNELYCGVALLVAYTMVVLGLLVLHFFKVDLVLAYRKLSPCLKTSSDGKLYDAYVSYLHGSDQSKCSATAFALDVLPEVLEDHLGYKLFISGRDDLPGAAVPDVIADTVGKSRRLIIILTSQAFTKPINSATVGLLQGISAEHVSINNNNTAIDPAVSCPAGTYGLSWGPYEWWMGLYDALVKEGLQVILVQVGAKVDDAQLPESLRYICCTQGVLHWKQHYSTKPDAKFWKQLRYRMPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJ50</accession>
    <name>A0A3B4EJ50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557492.1">
        <property type="nucleotide sequence ID" value="XM_017702003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJ50"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035334"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028167.2">
        <property type="protein sequence ID" value="ENSPNAP00000035334.1"/>
        <property type="gene ID" value="ENSPNAG00000025228.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429924"/>
    <dbReference type="CTD" id="2357"/>
    <dbReference type="GeneTree" id="ENSGT01020000230438"/>
    <dbReference type="OMA" id="MMEQASY"/>
    <dbReference type="OrthoDB" id="6088892at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15116">
        <property type="entry name" value="7tmA_CMKLR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000034">
        <property type="entry name" value="G-protein coupled receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="C3A ANAPHYLATOXIN CHEMOTACTIC RECEPTOR-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00526">
        <property type="entry name" value="FMETLEUPHER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="52"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="372" mass="42271" checksum="81129E0383C2E026" modified="2018-12-05" version="1">MSLHNNSSAGNWSDEEYYDDDYYDDVHAELRKSLNIMSLIVYCLAFVLGVFGNGIVIWVSGFRMKKTVNTVWFLNLAVADFLFTAFLPLSVAYTAMDFHWPFGRFMCKLNSTLSFLNMFASVYILVVISIDRCVSVVRPIWAQNHRSVSRASVLSLGVWVFALILSSPYFVFRDTAPEDENENVINCFNNFVFSNDNETAEVEDLRLLRHRAMIITRFLLGFVVPFSIIVSCYAVIIHRLKRNRSMSGRTGRPFKIIAAVITAFFLCWAPYHILMLIEMANHLASNYSPTLEHVTTIGIPIATSLAFLNSCLNPLLYVFIGQDFKDKVRKSVLKVLETAFTEEVSRTNTCTNSMITSRCKEQGSKSFSDAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK75</accession>
    <name>A0A3B4EK75_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sine oculis homeobox homolog 1a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Sine oculis homeobox homolog 1b</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SIX/Sine oculis homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017565972.1">
        <property type="nucleotide sequence ID" value="XM_017710483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK75"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036857"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040335.2">
        <property type="protein sequence ID" value="ENSPNAP00000036857.1"/>
        <property type="gene ID" value="ENSPNAG00000028036.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434988"/>
    <dbReference type="CTD" id="404627"/>
    <dbReference type="GeneTree" id="ENSGT00940000156487"/>
    <dbReference type="OMA" id="TSHMNEN"/>
    <dbReference type="OrthoDB" id="3501850at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021984">
        <property type="term" value="P:adenohypophysis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043282">
        <property type="term" value="P:chordate pharyngeal muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002074">
        <property type="term" value="P:extraocular skeletal muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048935">
        <property type="term" value="P:peripheral nervous system neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014857">
        <property type="term" value="P:regulation of skeletal muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000063">
        <property type="entry name" value="SIX homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031701">
        <property type="entry name" value="SIX1_SD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390:SF13">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16878">
        <property type="entry name" value="SIX1_SD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 15" id="KW-0371">Homeobox</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="17">
        <location>
            <begin position="122"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="124"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="167"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="284" mass="31975" checksum="AB1A56291D620F92" modified="2018-12-05" version="1">MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYTHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENSENNNSGANKQSQLSPLDGGKSLMSSSEEEFSPPQSPDQSSVLLLQGNMSHPGAPAYTMSGLGATQTVHGMQGHPHQLQDSLLGPLTSSLVDLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EM50</accession>
    <name>A0A3B4EM50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Perilipin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EM50"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032027.2">
        <property type="protein sequence ID" value="ENSPNAP00000036853.2"/>
        <property type="gene ID" value="ENSPNAG00000028098.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182920"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.340">
        <property type="entry name" value="Flagellar protein FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.170">
        <property type="entry name" value="Perilipin, alpha-beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF11">
        <property type="entry name" value="PERILIPIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036881">
        <property type="entry name" value="PAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109775">
        <property type="entry name" value="Mannose-6-phosphate receptor binding protein 1 (Tip47), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036853.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="421" mass="45309" checksum="85604B88A9F48591" modified="2024-11-27" version="2">MSESEKSSQMETETPAAAQDQQSLMSRVSNLPLVSSACGAVSSAYSSTKDNIPLLRGVMDAAESGVRTLGAAASTGTKPILDRLEPQIAMVNEYAMKGLDKVEEKLPILHQPADKLVSETVDKVYHSVTGAKDVVVGAVLGGVEITRTAVNEGINTVMGTSLGQMVSSGVGLALTRSEDWVDQHLPLTEKELAALAEPLPNEEGGAALSSASSYFVRLGKLSARVRERALELSLIKARNARDATHAAVTQITSTLDLLEAARAARTTASQIGGAPEQLLKRWKEWKEGQPKEEEEQGTEEAQTTLVEDQGEQLEWRTLSMVRGLSEQLRMACASVVSSTQGLPGAVQEQLQNARRTAEDLHSSLGKTSTLTPLLLQQSRQQLTQVRQSLDGVTEYLLNNTPLNWLVGPFAPQLTEKRLSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKM3</accession>
    <name>A0AAR2IKM3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKM3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041780.1">
        <property type="protein sequence ID" value="ENSPNAP00000040213.1"/>
        <property type="gene ID" value="ENSPNAG00000012675.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157386"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000001">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000008">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000007">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF4">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043905164" description="Rab-GAP TBC domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="130"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="432"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="559"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="674"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="686" mass="76917" checksum="9C38104971B029BA" modified="2024-11-27" version="1">TFLSTNRTGWLFSWCALCADAGLKLLMARLEKLENGRQPADTVSLQTVGSQLDEESSLGSDSEVNGFPSSRETDKYGFIGGAQKYSAESAQDVPPEVLRQREVKWLDMLSNWDKWISKRFKKVRLRCQKGIPPSLRGRAWLYLSGGKVKKERNGEKFKELDSMEGDPKWMDVIERDLHRQFPFHEMFVSRGGHGQQDLLRVLKAYTLYRPEEGYCQAQAPIAAVLLMHMPAEDAFWGLVQICEKYLPGYYSAGLEAIQLDGEILNALLKRVCPIAYKHLDKHKIEPILYMTEWFMCAFSRTLPWASVLRVWDMFLCDGVKIIFRVGLVLLKSMLGTREKLKACPGQYETMELLRALEPKYMQEAYLVQQVLELPISVRNVEREHRVQLKRWRKTHGELRYKAAPRMHGARIIMDAEPHTRQDLRQKPTIIVHYPSAPELNPDFNRGKKRGTLRKNPTPAPNIPNPYALPSDHTSAQQAATANQSGQPSAAAEPPQNPAASKKTSQPLQQPSLTDQQGHVKELPPVEGLKLSPNNSVKQNSSAPNTSFIEPANPSTQPLYFPPPPSPPFNEPPPPPPPPPTPPLPPPTPPPLAPPVEVPLQNWRPHLQDYPPLHGLGSVTTPVPIPEDSEVHEIVENSTALAPPPTTDSAPLLTPRAPANKDFHATHDNDILCHSSESVNSSEDTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ILN2</accession>
    <name>A0AAR2ILN2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Member of the ionotropic glutamate receptor family, which plays a crucial role in synaptic organization and signal transduction in the central nervous system. Although it shares structural features with ionotropic glutamate receptors, does not bind glutamate as a primary ligand. Promotes synaptogenesis and mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis of cerebellar parallel fiber-Purkinje cell (PF-PC) synapses through the NRX1B-CBLN1-GRID2 triad complex. In the presence of neurexins and cerebellins, forms cation-selective channels that are proposed to be gated by glycine and D-serine. However, recent research disputes this ligand-gated cation channel activity. Cation-selective ion channel activity can be triggered by GRM1 in Purkinje cells.</text>
    </comment>
    <comment type="function">
        <text evidence="22">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14 22">Postsynaptic cell membrane</location>
            <topology evidence="14 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2ILN2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046051.1">
        <property type="protein sequence ID" value="ENSPNAP00000038909.1"/>
        <property type="gene ID" value="ENSPNAG00000016618.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155910"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051963">
        <property type="term" value="P:regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000024">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000068">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000045">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000040">
        <property type="entry name" value="Glutamate receptor, ionotropic, delta 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 22" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 22" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 22" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6 22" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 22" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="781"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="24">
        <location>
            <begin position="386"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="25">
        <location>
            <begin position="396"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="859"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="692"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="20">
        <location>
            <position position="615"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="20">
        <location>
            <position position="642"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="706"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00056512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="963" mass="107235" checksum="BECB944480A14185" modified="2024-11-27" version="1">LRLVALCDLPVVRNYWCLSKNQQKRAISLMNQGILALVSSTGCAAANALQSLTDAMHIPHLFIQRNGDGTPRAECQLNPSPDGERYTLAARPPVRLNDVMLTLVSELRWQKFIMFYDSEYDVRGLRGFLDQASRMGLDVSLQQVERNVSKVFSTLFSTMKTEELNRYRDTLRRAILLLSPRSAQVFIHQAVETNLASKDSHWVYANEEVSDTEIMELVHSALGRMTVIRQIFPLWRDTNIRCMRNNHRISSLLCDPQEGYLQSLEVSSLYLYDSVLMLANAFYRKLEDRKWHSMASLNCMRKSTKPWNGGWSMLDTIQKGRISGLTGLMDFRSNGANSHAQFEILGTTYSETFGKDVKRLATWDSIRGMNGSLKESRVESGMQGVTVKVVTLLEEPFVMVAENILGQPKRYKGFSIDVLDALAKILGFKYEIYQVADGKYGSPQPNGSWNGMIGELIKKRADLAISAITITPERENVVDFSKRYLDYSVGILMSKSVEKLNIFSLLAPFDLAVWACIAAAIPVVGVMIFLLRRIQSIRSQNTPGPHQPASVTTSFQSAIWIVYGAFVQQGGESIMSSVALRIAMGSWWLFTLIVCSSYTANLAAYLTVSRMDNAVRTFQDLSKQADLSYGTVRESAVYEYFRVKGTNPLEQDSTFAELWRTINKNKGQDNSVATPAEGIQKVKRESYAFLWDMAVVEYAALTDDECTLTVTGSSMSTKGYGLALQHGSPYRDLFSQKILELQEKGDLDILKQKWWPRKGRCDLDRGTEAQSEGRALRLHSFAGVFCILAAGLLLACLVAALETWWNGPRCRRAQPKEDKEVNLEQVHQRLTGLIDDDLAHKQLPGQSLEISALDMGSMGSMGSLGGMRQRQGSQEAVRDFPPGGMSVTSFLQEGSGVGRLPGRNLPLPLSSSTLPPSMRCKHRAPNGGLFRQSPVKTPMPIAYQAVPGGPIPEAMDSSHGTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IY32</accession>
    <name>A0AAR2IY32_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TAAR1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IY32"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074410.1">
        <property type="protein sequence ID" value="ENSPNAP00000042501.1"/>
        <property type="gene ID" value="ENSPNAG00000035636.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244823"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000279">
        <property type="entry name" value="Trace amine-associated receptor 16f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="11">
        <location>
            <begin position="24"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="41"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="37265" checksum="56D04A196E9E20F6" modified="2024-11-27" version="1">MSIQYCFPGVNSSCRKEVRTGPAYIFLFIVLSCVSVFTVFLNLLVIISISHFKQLHTPTNLLILSLAVADLLVGLIVMPVKIMELIDSCWYLGKIACSVSPIIYYLSMSASLCSLVFIAVDRYIAVSDPLLYSTRVTVCKISVFIIIGWSFCSFYAIIYLYTNDHLLLSQISGECYGECVVFVKDSWVIIDMVICFIAPCSVILVLYSIIFTVARRQARAVRAVKNGASKIQRAKPSNSSETKAAKTLGIIIFVYLACWIPFYVTSLSVESVTSSSMVWTVYGWLLYLNSSVNPLIYAIFYSWFRESAKCILTCRIFESSSSRFNLYPERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J1Q0</accession>
    <name>A0AAR2J1Q0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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                <person name="Pippel M."/>
                <person name="Winkler S."/>
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                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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    <accession>A0AAR2J3W0</accession>
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            <authorList>
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                <person name="Karagic N."/>
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                <person name="Howe K."/>
                <person name="Fedrigo O."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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    <accession>A0AAR2J6X8</accession>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
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                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds to MUC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J6X8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079322.1">
        <property type="protein sequence ID" value="ENSPNAP00000046097.1"/>
        <property type="gene ID" value="ENSPNAG00000033288.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193372"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000009">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="14">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000046097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="169" mass="19836" checksum="6FB720E492E8D0A8" modified="2024-11-27" version="1">MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLEWRDLKIVYKRYASLYFCCAIEDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLLGGEAQETSKKNVLKAIEQADLLQENLDFQIRLFPGVLGPNVSAESSGLFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JBZ8</accession>
    <name>A0AAR2JBZ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL33m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L33, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MRPL33</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546254.1">
        <property type="nucleotide sequence ID" value="XM_017690765.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JBZ8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079091.1">
        <property type="protein sequence ID" value="ENSPNAP00000047754.1"/>
        <property type="gene ID" value="ENSPNAG00000036336.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108423459"/>
    <dbReference type="CTD" id="9553"/>
    <dbReference type="GeneTree" id="ENSGT00390000010130"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.120:FF:000005">
        <property type="entry name" value="39S ribosomal protein L33, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="entry name" value="Ribosomal protein L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052008">
        <property type="entry name" value="Mitoribosomal_protein_bL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_bL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="Ribosomal_bL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L33, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL33M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00471">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="65" mass="7499" checksum="B8148A5823C26AE7" modified="2024-11-27" version="1">MFLTAVNLAKSKSKTVLVQMMSAAGTGYCFNTKRGRLRDKLVLRKHDPFVNKHVLFFEKKKIRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JCW1</accession>
    <name>A0AAR2JCW1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Microtubule-binding protein required to ensure proper cell division and nuclear envelope reassembly by sequestering the endoplasmic reticulum away from chromosomes during mitosis. Probably acts by clearing the endoplasmic reticulum membrane from metaphase chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560430.1">
        <property type="nucleotide sequence ID" value="XM_017704941.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JCW1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058849.1">
        <property type="protein sequence ID" value="ENSPNAP00000049775.1"/>
        <property type="gene ID" value="ENSPNAG00000034761.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108431646"/>
    <dbReference type="CTD" id="641577"/>
    <dbReference type="GeneTree" id="ENSGT00940000158794"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF39">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="177"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="256" mass="29531" checksum="2DF8E4F4106547ED" modified="2024-11-27" version="1">MVSWIISRAVVLVFGTLYPAYYSYKAVRTKNVKEYVRWMMYWIVFALYTVLETITDLSMAWFPLYYELKVAFVIWLLSPYTRGASLIYRKFLHPLLASREREIDDYIVQAKERSYETMVSFGRQGLSIAATAAVSAAVKGQGAISERLRSFSIPDITQVPSDQSAFGRSFQRAGQNLNPSIEDSEHYPYKQEEDGHEEEVDGEYLEDESALPKGLRRSQSVKITRSKVVRKEPRYGSLKIKSRRRPMITSSTFEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLX9</accession>
    <name>A0AAR2JLX9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adiponectin receptor 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537413.1">
        <property type="nucleotide sequence ID" value="XM_017681924.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537414.1">
        <property type="nucleotide sequence ID" value="XM_017681925.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLX9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055883.1">
        <property type="protein sequence ID" value="ENSPNAP00000053028.1"/>
        <property type="gene ID" value="ENSPNAG00000034329.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108410708"/>
    <dbReference type="CTD" id="436740"/>
    <dbReference type="GeneTree" id="ENSGT00940000154563"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033211">
        <property type="term" value="P:adiponectin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF40">
        <property type="entry name" value="ADIPONECTIN RECEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="42619" checksum="784CF16744D7C916" modified="2024-11-27" version="1">MSGRNGSANDADCRISEECHVPADVELMELGPLLEEPGPLVANTLLSEQVESDLAVEDNDDDEVGEVLTLPLQAHHAMEKMEEFVHKVWEGRWRVIPFHVLPEWLKDNDYLLHGHRPPMPSFRACFGSIFRIHTETGNIWTHLLGLILFLCLGTLTMLRPNMYFMAPLQEKVVFGMFFLGAVLCLSFSWLFHTVYCHSEKVSRTFSKLDYSGIALLIMGSFVPWLYYSFYCSPQPRLIYLTIVCVLGIAAIIVAQWDRFSTPRHRPTRAGVFMGLGLSGIVPTMHFTIEEGFIKATTVGQMGWFYLMGAMYITGAGLYAARIPERYFPGKCDIWFHSHQIFHVLVVAAAFIHFYGVSNLQEFRYGLEGGCTDDTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JRW8</accession>
    <name>A0AAR2JRW8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">B3GALT2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JRW8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044720.1">
        <property type="protein sequence ID" value="ENSPNAP00000052616.1"/>
        <property type="gene ID" value="ENSPNAG00000030894.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155117"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045821">
        <property type="entry name" value="B3GT2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF19">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19341">
        <property type="entry name" value="B3GALT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-1,3-galactosyltransferase 2 N-terminal" evidence="14">
        <location>
            <begin position="59"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="390" mass="44689" checksum="9BFCA7587F4E3A28" modified="2024-11-27" version="1">MQWKWRHCCTSITGFLVLLLCVGVFLFISEYSLLARKKDHQVNSVTHTVQKLERNHSVTKQNMWTEEATSPFPATSEGNLSSILEDSSQQGLKNSVESNASFSEEIIAESILKVGPYTYIINEEDKCQEKDPFLVLLITVEPQNNQSRDAIRQTWGNESVAEGVGFVRLFLLGIREGPTSQLQSSIEAESQQYHDIIQQNYIDSYYNLTIKTLMGMHWIATYCPGASYVMKTDSDMFINTEYLIQKLLNPSLPPRLKYFTGNVMYNEPPHRNKNSKWYISEELYPDDVYPPFCSGTGYVFSGDLAELIYRTSLSIRRLHLEDVYVAICLAKLKIEPVPPPNQSLFNVNRVPYSSCKYSNLITSHGFLPTEIIKYWQDLQTNKHNACDHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JT86</accession>
    <name>A0AAR2JT86_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glutamate decarboxylase 1</fullName>
            <ecNumber evidence="4">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:17786"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JT86"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069239.1">
        <property type="protein sequence ID" value="ENSPNAP00000055225.1"/>
        <property type="gene ID" value="ENSPNAG00000019728.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155526"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009449">
        <property type="term" value="P:gamma-aminobutyric acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.170:FF:000003">
        <property type="entry name" value="Glutamate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000241">
        <property type="entry name" value="Glutamate decarboxylase isoform 67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF5">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 13 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="36"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="606" mass="68471" checksum="4B1D4B64C6460CE4" modified="2024-11-27" version="1">PGLDFGSSSSGTTQRGTQARAHCGTEPVKCAMFGHARSKFQSSSPSPNNPGFLQKNNNVDEKGRIVGPFTEQPSPKNLLTRDNERGSRFRHTETDFSNLYARDLLPAKNGEEPTIQFLLEVVDILTNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDQPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQLTLKKMREIVGWPNGEGDGIFSPGGAISNMYSVMVARYKHYPEVKTKGMTAAPRLVLFTSEHSKITWKPQKFSHHYSIKKASAVLGFGTENLILLKTDERGRVIPADLEAKVIDAKQKGCVPLFVNATAGSTVYGAFDPINEIADICEKYNMWLHVDGAWGGGLLMSRKHRHKLNGVERANSVTWNPHKMMGVPLQCSAILVREKGLLQGCNSMCAGYLFQPDKQYDVTYDTGDKAIQCGRHVDIFKFWLMWKAKGTSGFEKHIDRCLELSEYLYNKIKNREGYEMVFQGQPQHTNVCFWYIPPSLRDMPDREEKRQRLHKVAPKIKAMMMECGTTMVGYQPQGDKVNFFRMVISNPAATRSDIDFLIDEIERLGQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JU67</accession>
    <name>A0AAR2JU67_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">hypoxia-inducible factor-proline dioxygenase</fullName>
            <ecNumber evidence="9">1.14.11.29</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-prolyl-[hypoxia-inducible factor alpha subunit] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[hypoxia-inducible factor alpha subunit] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:48400"/>
            <dbReference type="Rhea" id="RHEA-COMP:12093"/>
            <dbReference type="Rhea" id="RHEA-COMP:12094"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.29" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017555289.1">
        <property type="nucleotide sequence ID" value="XM_017699800.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JU67"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049988.1">
        <property type="protein sequence ID" value="ENSPNAP00000053401.1"/>
        <property type="gene ID" value="ENSPNAG00000008631.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428652"/>
    <dbReference type="CTD" id="112399"/>
    <dbReference type="GeneTree" id="ENSGT00940000158745"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160082">
        <property type="term" value="F:hypoxia-inducible factor-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000005">
        <property type="entry name" value="Egl nine homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051559">
        <property type="entry name" value="HIF_prolyl_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907">
        <property type="entry name" value="EGL NINE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907:SF28">
        <property type="entry name" value="PROLYL HYDROXYLASE EGLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="12">
        <location>
            <begin position="163"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="285" mass="32049" checksum="D453ACD854AB9FB9" modified="2024-11-27" version="1">MEEASVRSGGEDSAVGEGARGAGRRRRRSGDNTEDGVPRELRQPRSNRPVSSERRRLNTHKLVLQYVAPCMKSYGLCIVDNFIGNRIGERVLHEVRRIHDGGRMQDGQLACQTRGKSKAIRGDKIAWVDGTEAGCHNIGFLLSRMDKLITFADGQLGSYKIRGRHKAMVACYPGNGAGYVKHVDNPNADGRCVTCIYYLNKNWNAKEHGGILRIFPEGKSYVADIEPLFDRLLLFWSDRRNPHEVQPSFATRYAITVWYFDSEERAEAKRRFKDLTASSQGSTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JXF6</accession>
    <name>A0AAR2JXF6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kinesin-associated protein 3b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JXF6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068208.1">
        <property type="protein sequence ID" value="ENSPNAP00000056690.1"/>
        <property type="gene ID" value="ENSPNAG00000029466.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003574"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008658">
        <property type="entry name" value="KAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05804">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="601"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="87305" checksum="8439F633B0183C57" modified="2024-11-27" version="1">TPKQKMVLQGFFTLFFFSCFVFFRKVKPGNIDVHPTEKALVVHYEVEASIRGERGDAVHEERKECQKIIRLKSLNASTDVALLARKVVEECKLIHPSKVAEVEHLLFYLQNRKKPGKEKKLMKPRELTPFEGMEIDEEANINKIDEYVELLYENIPEKIRGSTLILHLARNPDNLEELLRNETALGALARVLREDWKQSVELATTIVYVFFCFSSFSQFHGLITHYKIGALCMNIIEHELKRYDLWQDELDKKILNIPLRDEDPDNLALRKDHEKAYKKYQSLLVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHLLVKTLDRDSEELLVLVVSFLKKLSIFLENKNDMAELDTVEKLARLVPCEHEDLLNVALRLLLNLSFDTGLRSKMVQVGLLPKLTALLGSESHKQIVMCILYHISIDDRSKSMFAYTDCIPQLMKMVFEHGEERIDSELISFCINLAANKRNAHIICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPLKQLFLDYVGDLAAQIGPDEDEEFVIECLGTLANLTIPDLDWELLLREYNLVPYLKDHLKPGSAEDDLILEVVIMIGTVSMDDSCAVMLAKSGIIPALIELLNAQQEDDEFVCQIVYVFYQMVFHQATRDVIIKDTQAPAYLIDLMHDKNTEIRKVCDNTLDIIAEYDEEWGRKIQSEKFRWHNSQWLEMVESRQMDEAEPYLYGDDPDGLEQADLFYSTGKSRISNLSLSLSLSLSLSGTILGQYIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1G7</accession>
    <name>A0AAR2K1G7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">LSM1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in the degradation of histone mRNAs, the only eukaryotic mRNAs that are not polyadenylated. Probably also part of an LSm subunits-containing complex involved in the general process of mRNA degradation.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Probably involved with other LSm subunits in the general process of degradation of mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with SLBP; interaction with SLBP occurs when histone mRNA is being rapidly degraded during the S phase. LSm subunits form a heteromer with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017543229.1">
        <property type="nucleotide sequence ID" value="XM_017687740.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1G7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082042.1">
        <property type="protein sequence ID" value="ENSPNAP00000058163.1"/>
        <property type="gene ID" value="ENSPNAG00000037298.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108414839"/>
    <dbReference type="CTD" id="27257"/>
    <dbReference type="GeneTree" id="ENSGT00730000111133"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000021">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="5"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="133" mass="15188" checksum="EF205F5BA1928A11" modified="2024-11-27" version="1">MNYMPGTASLIEDIDKKHLVLLRDGRTLIGILRSIDQFANLVLHQTVERIHVGKKFGDIPRGIFVVRGENVVLLGEVDLDKECDQILQRVSIEEILQEQRVEQQAKQESEKRKLQAVKERGLSIPKADTLDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K6L3</accession>
    <name>A0AAR2K6L3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Unique cartilage matrix-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the negative control of osteogenic differentiation of osteochondrogenic precursor cells in peripheral zones of fetal cartilage and at the cartilage-bone interface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UCMA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K6L3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055208.1">
        <property type="protein sequence ID" value="ENSPNAP00000058087.1"/>
        <property type="gene ID" value="ENSPNAG00000037392.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000011492"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0031012">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048706">
        <property type="term" value="P:embryonic skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045667">
        <property type="term" value="P:regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031386">
        <property type="entry name" value="UCMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28647">
        <property type="entry name" value="UNIQUE CARTILAGE MATRIX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28647:SF2">
        <property type="entry name" value="UNIQUE CARTILAGE MATRIX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17085">
        <property type="entry name" value="UCMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0765">Sulfation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="49"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="106" mass="12724" checksum="5BAF882B93E1C5B6" modified="2024-11-27" version="1">CCSTVMTVLSCCSMKLCICSQKQGLILRNSALTTKPVLFLPSEQRQRLAADERKREYHEEQRNEFESYAEEEHDEQDERTREKTEQWREFHYDGLDPSYEYNRHTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KB58</accession>
    <name>A0AAR2KB58_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KB58"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052424.1">
        <property type="protein sequence ID" value="ENSPNAP00000061553.1"/>
        <property type="gene ID" value="ENSPNAG00000033754.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163847"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin heavy variable 9-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="93" mass="10681" checksum="EC26C6FC36542EF7" modified="2024-11-27" version="1">SHKLTCACSGFSVGGTNMHWIRQAPGKGLEWIIYYHSNSYKSSAQSVQGRFTASKDSSNLYLHMSQLKPEDTAVYYCARDSLWWNSYRQLHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KH72</accession>
    <name>A0AAR2KH72_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">IGKC</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KH72"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067205.1">
        <property type="protein sequence ID" value="ENSPNAP00000061814.1"/>
        <property type="gene ID" value="ENSPNAG00000030501.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="110" mass="11989" checksum="DF7E026EDC0E5042" modified="2024-11-27" version="1">FPKGVAAPTLTVLPPSSVELQQGKATLVCLANKGFPSDWRLSWKVDGSSWSSGVSQSPGLLQKDDGLYSWSSTLTLQEDQWLKKTVTCEATMDSQTPVSQTLRREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHT8</accession>
    <name>A0AAR2KHT8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Integrin alpha-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ITGA6</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHT8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062773.1">
        <property type="protein sequence ID" value="ENSPNAP00000063860.1"/>
        <property type="gene ID" value="ENSPNAG00000003629.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155353"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
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    <sequence length="1091" mass="120792" checksum="50D848BAFA70CFB5" modified="2024-11-27" version="1">MGALGVCLWLGVWICPLLLWAGAGAFNLDTQNVLRKNGDSGSLFGFSIALHRQLQPADKRILLIGAPRAKALANQGANITGGLYSCDITTQRDDCDRIEFDNDADVRVENKENQWMGVTVQSQGPGGKIVTCAHRYQRRLFVNTPQESRDITGRCYVLSQDLSIDSQSDEDGGNWKFCEGRARGHERFGACQQGVAATFTKDYHYVIFGAPGAYNWKGIVRVEQKNNTLLEMGFYDDGPYEVGDESRLESDLVPVPANSYLGFSLDSGHSITEKGKLIVVSGAPRANHSGAVILLKKAGEVSTMLSPEHILEGPGLASSFGYDVAVVDLNGDGWQDIVVGAPQFFQRDGEVGGAVYVYINKAGKWSSVVPVRLNGTKDSMFGLAVENIGDINLDSYEDIAVGAPYADEGSGRVYIYHGSVDGINTKPVQILEGRHHGIKMFGYSLAGNMDLDENSYPDLAVGSLSDAVIVYRPVISIKKEIKATPREIDLTKKTCGNSICLNVEACFTYKANPATYNPKLMISYTLETESHRRKQGLPSRVSFLDKKPTDQDFQSTGTLELRGQNKRACYKAKLRLQENIKDKLRALPIEVSVAIDSAKRGKRQNSLPELVPVLDSSLPSKISTEVNFLKEGCGTDNICQSNLQLQYRFCYRASNQDIFPPLPVTNGVAVVSLSDQKDIALEVTVKNRNGDDAHEAKLVGQFGDLLSYSGFRSHRTTDKQVICVANQNGSQADCELGNPFKRDSEVTFYIILSTAKISLSTTEVEVDLQLETTSLQEGLGKVRAKAKVMIELLLSVQGVAKPSQVYFGGDVRGESAMKSEEEAGSLIEYEFRVINLGKPLKSVGTAFLNIQWPKESAVGKWLLYLVKISSKELEEITCSPETEINPLGLTEGSMVSRQKREIGKSRLSEENKMSVFTERRKYKSLSCFGDAKCVEIKCPLLNLDSSADIILRSRLWNSTFLEDYASYNYLDLIVKASISLDVSAQNIVLKNPETQVRLTVFPEYTVSHYGGVPWWIILVAVLAGILMLALLVLILWKCGFFKRSKNDDSIPKYHAVRIRKETRHFKDDNVMFDPFEKKQWMTTWSENESYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHY8</accession>
    <name>A0AAR2KHY8_PYGNA</name>
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        <recommendedName>
            <fullName evidence="8">ELYS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="primary">AHCTF1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037397867.1">
        <property type="nucleotide sequence ID" value="XM_037541970.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSPNAP00000062069.1"/>
        <property type="gene ID" value="ENSPNAG00000016765.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108410654"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="ELYS-bb"/>
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        <property type="entry name" value="ELYS/MEL-28_NucAsmblyFactor"/>
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        <property type="entry name" value="ELYS_dom"/>
    </dbReference>
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        <property type="entry name" value="ELYS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21583:SF8">
        <property type="entry name" value="PROTEIN ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13934">
        <property type="entry name" value="ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16687">
        <property type="entry name" value="ELYS-bb"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELYS beta-propeller" evidence="5">
        <location>
            <begin position="1"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="ELYS-like" evidence="4">
        <location>
            <begin position="723"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1070"/>
            <end position="1094"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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            <end position="1149"/>
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        <location>
            <begin position="1191"/>
            <end position="1216"/>
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            <begin position="1247"/>
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        <location>
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        <location>
            <begin position="1519"/>
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            <begin position="1582"/>
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    </feature>
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        <location>
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            <begin position="2443"/>
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        <location>
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            <begin position="1452"/>
            <end position="1480"/>
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        <location>
            <begin position="1643"/>
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        <location>
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        <location>
            <begin position="1925"/>
            <end position="1944"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1945"/>
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    </feature>
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        <location>
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        <location>
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        <location>
            <begin position="2109"/>
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        <location>
            <begin position="2127"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="2375"/>
            <end position="2390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2517" mass="278728" checksum="6535F5DAA45D6A89" modified="2024-11-27" version="1">MRDLAAQVTSSLLRFPEVTVDALGEDEVTLDSVLHGKFTVGRSGLAWLACGPQLEVVHAVTGERFSAYRFSGVTEHPPTILAVRDFSWLKRSGLLVGLEEDEGSMLCLYDLGISRVVKAVVMPGRITAIEPLVSYGGASTSTQHLHQSLRWFFGVAAVVTDVGHVLLVDLCLDDLSCTQSELEASDLEVVNKSPSEIPRLREGVTQQGRHLCLQLCSPTGTGATALQYIHRTNQLAVGFSDGYLQLWNMKTLKKEYHSQLEGGRVPVYAFTFQEPENDPRNCCYLWAVQSAQDHEGDVVSLHLLQLAFGERKCLTSGKILYEGLEYCEERYSQDLNGTAFPLRAQATNTRLLSCQTIEKFRHHPDRDDSMSEVASPDTSVSMFTWQVKSYGQGQPSTFVGVFDINRWYHAQMPDSLRAGESLRNCPYLAIWSLDSVVEMTAPSVLLDMVVHERSLSRGLPYTCPPPEQFFNPTTYNFDASCLLNAGIVHLTCSGYQKETLSFLKKSVPSLSDGIANGYSRCLMSGLLAPRLADVQPSSLSQEEQLDAILTTAVETSSLGLITGCIKQWTAEEQPGSAVKLRYILEWAWDKVVHTKEELDSICAPLFDSSSNFTDPQTMQLLQHSQRLLSNLSTIFHCLLNEAQELTQRGLIGLMNKSMVSSLISQYAQVLLWFCRTGLLPESSDDDVLQISRPFYSFSVIKNYYMGQREELQRLAKDKWSADCLMIDGLVAQCGERLTELWKRDEGGTGQYPPPSLHALLDLYLLENVEESAKHAIVIYLLLDVMYSFPNKTGASVESFPTAFAIPIGLVKLVQGLWLLDHHDHENSLELLLHPATSHSLWAWQHGRVLHALMCQGKHSIALRYLHVMKPPLSSTSQAKLCLSVLLHNRCLVEAWALLRQHANKLNLEDLLRFLYETCQELDLMKELLKLPLGLSEQECLERFLQGTGGFQNRELLMVHYLQQANYVPALQINQTLKMNLAADRDPKMKERSNTRNSILNQYGKVLPRAQRKLAIERAKPYQHPTTILREVSRPQPLSTVAKRSATETVLTRAAFIKNVLTKIEEVWVGKDATPEPSPQKSPREPEVLVPSPNPPTLHVPEAFVGTPINMLTKRMSRLFDLVVQPSSQTSPEASRTQGTPPRSISSWVAPKSITKAPELSLLQTPQVVKRARALAASAPVFSSFTPQSILRSSLRPTPLGTPSASPGRSVTPPLRPKESRITFMEEAGTPELPKSSVHWRNGLAADTEIGQLKRPVLPPEVGVEHWSEHSGEEEDDEKEPSLEEKEKPGQKVESESSSPVKELQTQDPRQVLPTLQRRPSLGQETSEVSIQSDTTLEFHDALSPGDSEKQRQERQLVVETSVDEEVVIRFPRAAESVRTLANDAPAASQPAEESTEEKSQENTSEVQGQLDASRVKDEEQSKEKGHTQDLHKDFMPVPHVEKPSSEPLLDEVLNHHPEVVKESNQDKAVHEEPALIKEAEPEPQESVCEPSTRAEELINEPPELTVIEDSEPGLQQPVTEASTIDTEPICESPSVLPSRELESPQEPAGEPVSEPFAEELENHETGAEDAVEIAGKVERKAEAMETSDLDDYVDRHLFGNDLSPPLTRSGSKDPSETWTSFNSEPDGLKESESEPSAVDDVDAPAANTQKSATSSEAATSESQSVVSLNDSDELSSADSEEESGEDEEEEDSGSEVEIIEEVEGNGRQHQPAVYLEELPPHEQFLQEQTTAVLSLVGSDAQLKMMDGVMEQDDGEGEVVMVGLAPADLDEDADVPVCFTELKPSTTLLVPLELAAEHQEILDGGALHALASEEPEEPLPGGSSTFSLILDAGDEEEEKVASEEKPSNEITQHPEHIECQPVANPDVLCVEYTEVPHELMEKMEDLPTAEAEPVVLAAPHVEQPDRLEVLEMKPMQMLESASAGEKGAHSHLNEGRENGDIKDSASAEDEDDEKVPDEKKAHGRTMHHIDVAVEDHELSTAGMEVIPVAEDHASRCDEGALPQNDKLKEALKPSEEHPEAPSRDEVAEDKQNKAETVQQSPSRRGKKAVKFPLLITDFEKDLSDEESRVPSTPRRVTRSSRQLQDFEVPVTPRRSTRKAEPELPHGLEMPSTKSSKPSSPSRTPQKATTRKGTRRTRNTNVGSDEEAQSMEESVINDVAVPQVRQSVRKALKSSVDLPEPQHEVPEGKAPEEPPKASASPSRVTRKSTRGLSLALESLPKDSAEDTVAPKQSVVTPPRSRRKTRAGTAEKANNLTFVVDEAQLQSVSRRLTRSQLWSHEEEPKKEQLVLEAPQDMESANPLANALMERLQDDRAKEGEVVTEIVRAKRRTTKSVSENQNKGVDIPAEVVSTETQDTTKKANVRRTWASKVPEPDSSPATDSFAFTSSLQQTRGRKKDSAKTDISVSEMLLSPPVTRTRRAAALAEEHHEDQIPSNDIAVQVEEVKTKKKRTTRTKAAPEPVPSVEVDLVSPLASPAQPAARTLKRAEEKDEPVLKMNLRRKRVMEAIFPKPVTRRKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KMC7</accession>
    <name>A0AAR2KMC7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037392897.1">
        <property type="nucleotide sequence ID" value="XM_037537000.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392898.1">
        <property type="nucleotide sequence ID" value="XM_037537001.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392899.1">
        <property type="nucleotide sequence ID" value="XM_037537002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KMC7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044538.1">
        <property type="protein sequence ID" value="ENSPNAP00000065450.1"/>
        <property type="gene ID" value="ENSPNAG00000036569.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413103"/>
    <dbReference type="GeneID" id="119263082"/>
    <dbReference type="GeneID" id="119263083"/>
    <dbReference type="GeneTree" id="ENSGT00930000151196"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF14">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="31"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="229" mass="25954" checksum="6C54008BACD43612" modified="2024-11-27" version="1">MPTTTRILPVSRKTGRRTKAGNKLSEKPPLYDANIPEPACRAELLKYWVSLSMDERTANKLLWLTEGGAKVSRMNDEVCPYLDRPERYEHSPQVLCKEVLWGSRGYWEVEYSGWVMIGVAYESEGRRNSDGPCGFGENEGSWSLGWVGSSYHAWHKGVNQELQVPQCSSIGVYLDQPAGLLCFYTVERSSAGKKEVKLLHRVKNPIKEKTLPGFWVGRQSSCRLLPKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KQ16</accession>
    <name>A0AAR2KQ16_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="15">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LYN</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KQ16"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080313.1">
        <property type="protein sequence ID" value="ENSPNAP00000064584.1"/>
        <property type="gene ID" value="ENSPNAG00000021099.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158011"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000553">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000036">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="56"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="122"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="240"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="505" mass="57465" checksum="59D03CFA616B7076" modified="2024-11-27" version="1">MGCAKSRGKNGTLEQTQNHTHHKNRYTKDPTQPGPNTKNSSLLPGQDLQSIDDGSAKQKVVVALYAYEAGNKTHLSFRKGEKLQVLDDNGDWWRAKSLTSGKEGLIPYNYVEAEESIESNDWYFKNIPRRDAERYLLAPANKPGAFLIRKSETTEGCYSMSVRDVNANGSAAVKHYKIRPLDDGGFYISPRITFKKMEELIKYYQKQADGLCRQLGKPCVKPKAEIQWDEDAWEISKENIRMIKRLGAGQFGEVWLASYNNTTKVAVKTLKPGTMSVEAFLQEANLMKTLRHDRLVRLFAVVTKTTPIYIITEFMANGSLLDFLKSPPGRKLQLPKLIDFGAQIAEGMAYIEKMSYIHRDLRTANILVSESLLCKIADFGLARVIEENEYSASEGAKFPIKWTAPEAISYGSFTIKSDMWSFGILLYEIITYGKIPYTGLSNAEVMARVHRGYRMPRPENCPNKLYEIMTSCWKAEPEERPTFDYMQSILEDYFTATEDQYQQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KQ38</accession>
    <name>A0AAR2KQ38_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cell adhesion molecule 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Synaptic cell adhesion molecule</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Homodimer (via Ig-like V-type domain). Interacts with FARP1. Interacts (via Ig-like V-type domain) with CRTAM (via Ig-like V-type domain); the interaction competes with CRTAM homodimerization and CADM1 homodimerization. Interacts (via C-terminus) with EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts (via C-terminus) with MPP2 (via PDZ domain). Interacts (via C-terminus) with MPP3 (via PDZ domain); this interaction connects CADM1 with DLG1. Interacts (via C-terminus) with PALS2 (via PDZ domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KQ38"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061525.1">
        <property type="protein sequence ID" value="ENSPNAP00000064609.1"/>
        <property type="gene ID" value="ENSPNAG00000034848.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156093"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008037">
        <property type="term" value="P:cell recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051606">
        <property type="term" value="P:detection of stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042271">
        <property type="term" value="P:susceptibility to natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05881">
        <property type="entry name" value="IgV_1_Necl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000200">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000184">
        <property type="entry name" value="cell adhesion molecule 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF2">
        <property type="entry name" value="CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043355580" description="Cell adhesion molecule 1" evidence="19">
        <location>
            <begin position="28"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="31"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="133"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="233"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="441" mass="48279" checksum="E9E289619BAF1C49" modified="2024-11-27" version="1">MASSGSGSLSIPLFICLVSAVLSKGATQTIPAQGQNLVTGNVSVIEGETATISCRVKNNDDSVIQLLNPNRQTIYFRDVRPLKDSRFQLVNFSDNELRVSLSNVTLSDEGRYVCQLYTDPPQEAYADITVLVPPGNPIIESREDVVSEGNETEITCTAMGSKPASTIMWMKGDQILQGEETVEETYDRMFTVTSRLRLTVSKEDDGVAVICIIDHPAVKDFQAQKYLEVQYKPEVQIVVGFPEGPTREGENLELTCKAKGKPQPHQINWFKVDDDVPSHAVITGSDLFIENLNKSYNGTYRCVASNLVGEAYDDYILYVYDVLATTPPPTTTTINPDAAQGTVSTIICCCLFSPSHFFFADSRADGGPQKIDHAVIGGVVAVVVFAMLCLLIILGRYFARHKGTYFTHEAKGAEDAADADTAIINAEGGHNNTDDKKEYYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KVR0</accession>
    <name>A0AAR2KVR0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD repeat domain 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">WDR13</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017573674.1">
        <property type="nucleotide sequence ID" value="XM_017718185.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KVR0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044871.1">
        <property type="protein sequence ID" value="ENSPNAP00000068355.1"/>
        <property type="gene ID" value="ENSPNAG00000014009.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439660"/>
    <dbReference type="CTD" id="64743"/>
    <dbReference type="GeneTree" id="ENSGT00940000153634"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="232"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="468"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="102"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="55561" checksum="E6E3396FD8483EEC" modified="2024-11-27" version="1">MSLSPVLSSGPGVNPAALSLCGMAAVWQQVLAVDARYNAYRTPTFPQFRTQYIRRRSQLLRENAKCGFEPGLRRQYLRLRSQLLALRYGPLSEQSSFRASSVRSSRTTLDRMEDFEEDPRAQGARGHRRSVSRGSYQLQAQMNRAVYEERPPGSLVPTSVAEASRAMAGDTTLSENYAFAGMHHIFDQHVDSAVPRLQFANDDKHLLACCSLDGTLSIMTLSPSPPTVKVTLKGHAGPVTDFAWSLSNDIIVSTSQDGTLRIWNTEDGRCIREVVDPEASELLCCTFQPMNNNLTVVGNSKHLLQVVNISTGKKVKGGSSKLTGRVLSLSFDAPGRILWAGDDRGSIFSFLFDMATGKLTKAKRLVVSEGSSISSISARSWISREARDPSLLINACINKLLLYRVVDNEGTLQLKRSFPIQHSSQPLHSIFCPLMSFRQGACVVTGSEDACVYFFDVERNTKAIVNKLQGHSGPVLDVSFNCDESLLASSDATGMVIIWRREQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KZK0</accession>
    <name>A0AAR2KZK0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037388533.1">
        <property type="nucleotide sequence ID" value="XM_037532636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KZK0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046633.1">
        <property type="protein sequence ID" value="ENSPNAP00000069690.1"/>
        <property type="gene ID" value="ENSPNAG00000012724.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414885"/>
    <dbReference type="CTD" id="559198"/>
    <dbReference type="GeneTree" id="ENSGT00940000155777"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106018">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14532">
        <property type="entry name" value="PTP-MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000135">
        <property type="entry name" value="Myotubularin related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048994">
        <property type="entry name" value="PH-GRAM_MTMR6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF124">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21098">
        <property type="entry name" value="PH-GRAM_MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="7">
        <location>
            <begin position="125"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="544"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="589"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="547"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="4">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="337"/>
            <end position="343"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="635" mass="72049" checksum="97FB7F20AA61723C" modified="2024-11-27" version="1">MEHIRTPKVENVRLIDRLTPRKSVVGTLYLSSTHTIFVENSDARRETWVLHSLVGSIERPPTTPSGSPLVIRCKNFQVYQLLLPQEKDCVDIQASLIRLSRPDRYGELFCFSFQPNVDKEEREKAWGFLDLHAEFSRMGIPSNLWHITPANHEYRVCDTYPSELFVPKSASPAVIVGSAKFRSRGRLPVLSYFHRDTSAAVCRSSQPLSGFSARSLEDEQMLQAIMKSNPASEYMYVVDTRPKLNAMANRAAGKGYENEDHYTNIKLHFIGIENIHVMRNSQQKITDVGDMRSPSMGDFLWGLENSGWLKHIKAVLDAGVFIVKAVAEEGVSVLVHCSDGWDRTAQACSVASVLLDPFYRTIRGLMVLIEKDWVSFGHKFSHRYSHLEGDPKEVSPVIDQFLECVWQLMEQFPCAFEFNERFLLQLHTHIHSCQYGNFIGNSQKERRDLRINERTHSVWPHLWENRAEYINPLYRADHSQSKGILRPVTTPYCFKFWKGMYSPAERGVALRQSPSDSLNAVREESQQLEEELSAHQERLAQLVGRKTSGETRKRNGVTERSHRLIPKDSEVSSPQDYFTCVSHAPSFTLPLKSPTTPKSSDPDDLSACSDLESGVADLSSHASSACDDAKDPDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L307</accession>
    <name>A0AAR2L307_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555494.1">
        <property type="nucleotide sequence ID" value="XM_017700005.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L307"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063907.1">
        <property type="protein sequence ID" value="ENSPNAP00000070978.1"/>
        <property type="gene ID" value="ENSPNAG00000033500.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108428750"/>
    <dbReference type="CTD" id="405792"/>
    <dbReference type="GeneTree" id="ENSGT00940000159483"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="120"/>
            <end position="177"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    <sequence length="404" mass="43221" checksum="54A9810BD82ABBB2" modified="2024-11-27" version="1">MAYPQGFLYQPAAPLALYSCPPPYGARAEELSRSSSSSSSSSSSSSSSCSSGSAFAPYAGSTAFTSPAPFSSLQYGAEPAPLSAFVGSPYDPGPGVGGTMGFPPYGGALGPFPPLDPAYRKNATRDATATLKAWLSEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWIPRNRSEDEEEEENIDLEKHEEDDEPLKATEAATEASKELGGLPICPSSITCESHTESPSDEADRTTEREKRASPLPTAPSPEPRAPGQLPGPGEPAQPPNPPPKPKLWSLAEMATSDKGNSERAQPCVRTHSPAPLTPPRAHHCHFPRVQHLYCAPAAFYPAFTNYSALSHLSTSPAPGQPRAGGLRAGAVLRESALRAPTATDTRRESAFEHRLGTANVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L388</accession>
    <name>A0AAR2L388_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Neurobeachin-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NBEAL1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat neurobeachin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000046986.1">
        <property type="protein sequence ID" value="ENSPNAP00000070995.1"/>
        <property type="gene ID" value="ENSPNAG00000013975.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 30"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:membrane"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:myeloid cell differentiation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Beach"/>
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        <property type="entry name" value="PH_BEACH"/>
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        <property type="entry name" value="neurobeachin isoform X1"/>
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        <property type="entry name" value="Neurobeachin-like protein 2"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
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        <property type="entry name" value="WD40_rpt"/>
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    </dbReference>
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        <property type="entry name" value="NEUROBEACHIN-LIKE PROTEIN 1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Beach"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
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        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BEACH"/>
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        <property type="entry name" value="PH_BEACH"/>
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043860089" description="Neurobeachin-like protein 2" evidence="9">
        <location>
            <begin position="23"/>
            <end position="2753"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH-type PH" evidence="11">
        <location>
            <begin position="1943"/>
            <end position="2040"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="10">
        <location>
            <begin position="2051"/>
            <end position="2343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="2547"/>
            <end position="2588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="769"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1332"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1436"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="776"/>
            <end position="788"/>
        </location>
    </feature>
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        <location>
            <begin position="1344"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1376"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1440"/>
            <end position="1455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008498"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068540"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2753" mass="309422" checksum="F6B8EAB6F0B12D0A" modified="2024-11-27" version="1">SMSLFNFHFSLSLSLSLSLSLSLSLSLSLSLSQRDPDYLRLWLEIFIGSYDRCLDVDFEKPPSRYSCTVPPVLSLLPDNILQVLRVQLLQCVQKASAGLEQEQQHLALLLLKFLIIICRNLSNVEEIGTCSYINQIITMTTLYIQQLKSKTKEKELADQTQAEEFVRHALAFCESLYDPYHNWRHRIHRQQLGTVEKSRQKFKAAPLTVEFVPFFYQCFQESEHLKDSLKCCLLHLFGAIVAGGQRNALLAISPATLEVLLAVLGDSECMGGVGDGEDWESEAPDRKALLTLACLREVVHSLLASSSDQRQVEISSVLESYFKLLNSDPAATAQAKGRPAPPRSRHWESRFVALQVKMLETIQDMFECSDRPVLQAIFLNSNCFEHLTRLLQNSKLVNARCTSADKEQKDLTNRLLTGESETQVFQGRLDSLAVATIKALTTVMHKSPAAKEVFKERIGYTHLYEVLVSLGQPSRHLLKELMNMAVEGEHSSVGLLGISNVDPLLLLIQWLPELDSSELQIFTADWLRRICGLNRCTRATCVNARMSMRLLSTLEKHTRLHRACGESLVALLGSLGGQSLSGSELHQLLSFLRQDDPSRPHPYVGPVLRSLLAMLRKHGLESAMQYFDLTPPMAGIAVPKVSRWPGFAFSFLAWLSLDQDQLGPPSKGEKRKQLYSFFTPGGTGFEAFISSAGALVVAVCSKKEYVTVMLPDYCFCDSLWHFIGVVHVPGKRPFGQSLVYIYVDGQQKLSAPLKYPTMTEPFTSCCIGSAGHRTTTPPPSQIPDPPLSDAPSGRSSLGSILSPQTWGGLLGGKPESLTKLISAGTQDSEWGSPTSLQGQLGSVMVFHDALQTSHVKALFSAGPNCISPFKSQEAELGELSSKLLLHYSPKACRNPICLDLSPNLLHGRLTGNKVVNWDIKDMINCVGGLPVLFPILEQLTLLTPDPADTSGSEFITPETATPADGDWVILPSNRASEARLEKNLAATFLLVVKHFLQRHTVNQETLLHCHGAATLGTLLQKVEDLPLLSDVVLVAVQLLIEQVTFEKNPQLLQQLHTHLLFNFNIWNQGDFPLRIGHIQYMSTVIKDSRKQFRKKYGVQFLLDTIRLYYGSGNREVDLSEDDIRTIRASLCGLLKYYISKGTTQEEIQSILGYIAAIGDDEQLCGMLELLLTLLQTSAARDQLFLLLFEPGAADSCYALLLNHKHSDRLRELVFKVFERMLKCDRVYEKSKQRLRLREVGYSGLTLLFSDLQLTPSLVKCLINQVLHTDTVVNYKDLMALVQLTHRAGPSVRLLVCKRLYSLLQSQSDAALQISRQLCWQDSLMRLLVRSPGAEGSTGSFRADSGSLGSLELGRSGGGNRLEPPLDRRTLPSTEGRLNDERDSVSDSRSIDSLDNGDMLSLTETPGDAPFNYYVNKSPKTGKAGGLSLDLSQVHLFDQGDSGSQTPGSMPSTPSPLENAKPFPGAAAERETSLPDDSFLFSDNVSLGESFNSRAEDELWRILLEIVLCVMWRGVGGSDDNAWLERGQVFSALTKLGTANELLLPVDHIKLSLLERMVEWAVNDNRESTAVTLPQHTENAVRLLHVVQDFLQAEGLVNPALWTEKVLEETVTLMDGLMVWYTSGSQWLQLSQVGLRLLLGFMAQPDPQVCAMATAKLNGILQTKSIDSQDEACFLLGRVEGILRRSVSEQKEETYSFLVPLLRTLLSKVHKLLYMELHLPSLPDTNGSPSFFEDFQLYCNSPEWRVYLDKYIIPYMKQYEIETFSQGHETMALFWKDCYEAFMINLHRRERERGESKIRFQEQFVEPFSRRGRQENLRYNSMLKQMHTQNSAILRQWKAARRGLMCERGPWVDKQQKEMNWKLSSAENYSRMRLKLVRNYNFDPHREASALRDNLGVQHQQINAESLLLEAVKQVKVSDLEEEILDLPEEEQSGSTQADDEDASQKEKLVMSEDCELVTVVDIYPGRLDITTHHIYFYDSSTEKEEGVGQDFKWPLSQIREIHLRRYNLRRSALEIFLIDQTNYFLNFKKEVRNKVYSRMLLLCSLSLHGTRSPQELLKASGLTQKWVNREISNFDYLMQLNTIAGRTYNDLAQYPVFPWILSDYTSGELDLSDPRVFRDLSKPVAVLNEKNAKAVREKYESFEDPTGTIDRFHYGTHYSNAAGVMHYLIRVEPFTSLHIQLQSGRFDCADRQFHSIPATWQTLMDNPNDVKELIPEFFYFPEFLENQNGFDLGRLQISKERVNDVVLPKWAKSPEDFIYKHRKALESEYVSAHLHEWIDLIFGYKQRGPAAVEALNVFYYCTYEGAVDLDAITDEKERKALEGMISNFGQTPCQLLKEPHPVRLSLEELEKRKSRLDSCPLSIFEHLTELKSFFVEGISDNCALVKAVVPKNQSHSFISQGSPDTLVTVSQNCLIGTHGWLPYDKTISNYFTFIKDPTVTSQKTQRFLAGPFSPGVEVTARLFVVSHDGKLLFSGGHWDNSLRVTSLLKGKTVGQHIRHMDIITCLATDYCGIHLISGSRDTTCMVWQVLQQGGAPVGLSPKPLQVLYGHTDEVVSVCISTELDMAVSGARDGTVIIHSVRRGQYMRSLRPPCESSLPVSITHLAVSWEGHVVVHTCVEGKATLKDKNALHLYSVNGKHLCSDPLKEQVTDLCVSGEYIVMGSEQGFLSIRDLYSLALCVSPMAMRVPIRCVSVTKEQSHVLVGLQDGKLIVVGVGKPAEVKNSFRNYITQRMHGSPLMNTLRMHAHTHLLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L6T3</accession>
    <name>A0AAR2L6T3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="2 12">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="2 12"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2L6T3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061134.1">
        <property type="protein sequence ID" value="ENSPNAP00000072308.1"/>
        <property type="gene ID" value="ENSPNAG00000000418.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154704"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12086">
        <property type="entry name" value="DD_cGKI-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05572">
        <property type="entry name" value="STKc_cGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000096">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000035">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000037">
        <property type="entry name" value="cGMP-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000046">
        <property type="entry name" value="cGMP-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002374">
        <property type="entry name" value="cGMP_dep_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031831">
        <property type="entry name" value="PKcGMP_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035014">
        <property type="entry name" value="STKc_cGK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF68">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16808">
        <property type="entry name" value="PKcGMP_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000559">
        <property type="entry name" value="cGMP-dep_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00104">
        <property type="entry name" value="CGMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0140">cGMP</keyword>
    <keyword evidence="9 12" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="117"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="235"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="373"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
        <location>
            <begin position="644"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="657"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="11"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="379"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000559-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000559-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="695" mass="79198" checksum="360A2D0E1D64306C" modified="2024-11-27" version="1">MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQVHKPTSALQEQQRTKRQAISAEPTALDIQDLSHVTLPFYPKSPESKELIKEAILDNDFMKNLELSQIQEIVDCMYPVEYDKESCIIKEGDVGSLVYVMEDGKVEVTKEGMKLCTMGPGKVFGELAILYNCTRTATVQTLTNVKLWAIDRQCFQTIMMRTGLIKHAEYMEFLKSVPTFQGLPEEILSKLADVLEETHYNDGEYIIRQGARGDTFFIISKGKVNVTREDSPNDTVYLRALGKGDWFGEKALQGEDIRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKGYEDAGAKAKYEAENAFFSNLKLVDFNIIDTLGVGGFGRVELVQLKSEETKTFAMKILKKRHIVDTRQQEHIRNKWPHWLSLLLLKCHHFERIILLIAHDMNLTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKITKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPDVSSPTDTSNFDSFPEDSEDPPPDDNSGWDADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLZ7</accession>
    <name>A0AAR2LLZ7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED31</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLZ7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046962.1">
        <property type="protein sequence ID" value="ENSPNAP00000075356.1"/>
        <property type="gene ID" value="ENSPNAG00000014326.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015531"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1340:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="137" mass="16525" checksum="6AF4ACD48B83EA6A" modified="2024-11-27" version="1">QMAGVLETEEQARNRFQSELEFVQCLANPNYLNFLAQRGFLREKPFVNYLKYLLYWKEPEYAKFLKYPHCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRTRLQQALAEQQQQQQQAAPPHGNPASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LR55</accession>
    <name>A0AAR2LR55_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Androglobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">COX4I1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LR55"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066240.1">
        <property type="protein sequence ID" value="ENSPNAP00000077147.1"/>
        <property type="gene ID" value="ENSPNAG00000035092.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014904"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053033">
        <property type="entry name" value="Androglobin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46298">
        <property type="entry name" value="ANDROGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46298:SF1">
        <property type="entry name" value="ANDROGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24695" checksum="FD20644196351833" modified="2024-11-27" version="1">MKQCRSSQQRKRNLYPVASLHRKDNRPLKSKNHYGPGDLKRSRFPIWPEWNEAEVSAEKWDAAKGAKDGKAGKSPLSVSQFFEDPEGKVELPASLKVHTWKRPSEYIVNKTLVVVENESTFDLTSANEHLLSSELMRWIISEIYIVWKVCSGAVGMASEAPNPWRPWEHIYSLCKVTKDHVPQYNIYGKYVVRLYWMVISTYGEIVTHSNLLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M3Q0</accession>
    <name>A0AAR2M3Q0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M3Q0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000087992.1">
        <property type="protein sequence ID" value="ENSPNAP00000081381.1"/>
        <property type="gene ID" value="ENSPNAG00000037363.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282554"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="222"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="696" mass="78601" checksum="0FD69F2C29D24FC6" modified="2024-11-27" version="1">MELDSLTDYLENTLRSTLENVAPLKIKRIRQKKLAPWYNDKTRTLKQTARKLERKWRHTKLEVFHTAWKDGLIEYRNALTEARSAYLASLIENNKYNPRVLFNVISQITQNQAGNEPEIPVTLTSEVFMDFFNNKIDNIRQQIQPTVSNSNPAWLSSDLADIEQNTAVEKNLETFYPLPQLELEKIISSANCTTCTLDAIPSKLLKEVLPVIINPILTIINSSLSLGHVPKAFKLAVIKPLIKKPNLDVTVLSNYRPVSNLSFISKILEKAVAQQLSSYLHKNHIYEKFQSGFRPHHSTETALVKITNDLFLASDQGCVSLLVLLDLSAAFDTIDHNILLERLENMVGVTGTALSWFRSYLSERYQFVRVNNLSSTYSKVRFGVPQGSILGPLLFTLYMLPLGTVISNHGVNFHCYADDTQLYISAKPDDQYRLKKMEDCVKDVKGWMSQNFLLLNSNKTEVLLLGPKLARSKSPDLILNLADFPATPGSAAKNLGVIIDSDLSFDQHIGSITRTAFLHLRNIAKIRNALSLRDAETLVHAFITSRLDYCNALLSGCTSRNLNKLQLVQNAAARVLTKTRKFEHISPVLSSLHWLPIKFRIDYKILLLTYKALHGLAPEYLQDLISHYEPSRPLRSQSAGLLIVPKILKVSAGGRAFSYKAPKLWNDLPETVRDSDTVSIFKTRLKTHLFSLAFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQG0</accession>
    <name>A0A3B4BQG0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serum amyloid A like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SAAL1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAAL1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017562124.1">
        <property type="nucleotide sequence ID" value="XM_017706635.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQG0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000669"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013473.2">
        <property type="protein sequence ID" value="ENSPNAP00000000669.1"/>
        <property type="gene ID" value="ENSPNAG00000007645.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432649"/>
    <dbReference type="CTD" id="113174"/>
    <dbReference type="GeneTree" id="ENSGT00390000004737"/>
    <dbReference type="OMA" id="NMFQELT"/>
    <dbReference type="OrthoDB" id="2156856at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901647">
        <property type="term" value="P:positive regulation of synoviocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052464">
        <property type="entry name" value="Synovial_Prolif_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424:SF23">
        <property type="entry name" value="PROTEIN SAAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23424">
        <property type="entry name" value="SERUM AMYLOID A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="54258" checksum="486C803857946A2F" modified="2018-12-05" version="1">MMDHKESCTEEVDDDESSSSSHPDSPEMDRNPSPPPEGAEESVEEADAIGSTVYSKHWLFSTLTRLIEMVTEQVSEQGEGLEELSEELEDELCKVWDMAMDKDVASFLQEFKAPDILLGVIAKSHCPRLTEICVGILGNMSCFHDTCVSLSQNADLCAVLILLLGDSDPPTLLETCRLLVTCVSQPDVAPMWMERIQQQSATCSNLCFIMRSSTNVELLMKVGELVDKLFDEDEGLMKSWLSELPSESDRDKDTHQDITSSLLEAAIQLRKESPEALEVYLHALQLLTTVHEGVQSLVSDGGCGEALWRFLCEVVCEDLCQPGDPPLILQEQKALLAPAVALLSVLHNSLQTDISLSLVCSLLRILYLHTENQQSLPNGEQGSRDGQKEGVRDEEDKDVQLQVLVEMAVELLSTILTDLPKDIISELVKQDDLTEKIWMSAFKNLLPQNITAAQRLVSTLSEVEPKLADILKKEYSISSEPDQTRLELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BT83</accession>
    <name>A0A3B4BT83_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="17">The full-length protein can bind eight Ca(2+) ions via the annexin repeats. Calcium binding causes a major conformation change that modifies dimer contacts and leads to surface exposure of the N-terminal phosphorylation sites; in the absence of Ca(2+), these sites are buried in the interior of the protein core. The N-terminal region becomes disordered in response to calcium-binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572179.1">
        <property type="nucleotide sequence ID" value="XM_017716690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BT83"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002853"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009732.2">
        <property type="protein sequence ID" value="ENSPNAP00000002853.1"/>
        <property type="gene ID" value="ENSPNAG00000009223.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438700"/>
    <dbReference type="CTD" id="334724"/>
    <dbReference type="GeneTree" id="ENSGT00940000155221"/>
    <dbReference type="OMA" id="FMENQEQ"/>
    <dbReference type="OrthoDB" id="37886at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019834">
        <property type="term" value="F:phospholipase A2 inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071385">
        <property type="term" value="P:cellular response to glucocorticoid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000007">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002388">
        <property type="entry name" value="ANX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF17">
        <property type="entry name" value="ANNEXIN A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00197">
        <property type="entry name" value="ANNEXINI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0041">Annexin</keyword>
    <keyword evidence="9 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="16 17" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0593">Phospholipase A2 inhibitor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="360" mass="39870" checksum="7788F425927E0B1C" modified="2018-12-05" version="1">MRSFQLCCPSLRRHSERTSIMSSMSFVQAFLQQTVYLGGPDDSTVQGTVGAASQFSASGDAAILDKAIKAKGVDEATIIEVLVKRSNDQRQQIKAAYQQASGKPLDAALKHALSGELEDVVLALLKTPAQYDAQQLKLAMKGLGTDEDTLIEILASRTNREIRDIKKAYKDEFKKELEDDIKSDTSGDFRNALLALCKAARSEDKTVNEDLADKDARALYEAGEKRKGTDCTVFIDILTTRSANQLRKVFQQYQKYSKVDVAKAIDLELKGDIENCLTAVVKCAGSKPAFFAEKLNLAMKGSGYRGKILTRIMVSRSEVDMVQIKQEYKKKYGKTLYKDILDDTKGDYEKILLAIVGSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BT95</accession>
    <name>A0A3B4BT95_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 7">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CA7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 7"/>
    <dbReference type="RefSeq" id="XP_017579204.1">
        <property type="nucleotide sequence ID" value="XM_017723715.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BT95"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001740"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011624.2">
        <property type="protein sequence ID" value="ENSPNAP00000001740.1"/>
        <property type="gene ID" value="ENSPNAG00000008342.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443210"/>
    <dbReference type="CTD" id="766"/>
    <dbReference type="GeneTree" id="ENSGT00940000159757"/>
    <dbReference type="OMA" id="CNSINIY"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF124">
        <property type="entry name" value="CARBONIC ANHYDRASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="9">
        <location>
            <begin position="99"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="358" mass="39838" checksum="7562D8DBF8DE1424" modified="2018-12-05" version="1">MEEPARCSPALIPKGANREVRRKQELPSNPASSASISCEGGVTTRARLPLLPVNHTALEIPPPVRAGIHLQLPQRDQSSPSVCTFLFTVQHLSSAMTGHRWGYGEDDGPSAWHKDYPIAEGPRQSPINIVPEEAVYDSNLTPITLNYDNCASVNISNNGHSVVVDFDDADDRSVMQGGPLDSTYRLKQFHFHWGGKGCHGSEHTVSGKTYTSELHLVHWNSARYSSFGEAVAAPDGLAVLGIFLERGDEHRGLHAITDALYMVKFKGTVADFRDFNPKCLLPRNLNYWTYPGSLTTPPLYESVTWIILAEPIVVSEKQMGKFRMLLFSGEEEEDRKRMENNFRPPQPLKGRTVRASFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVT1</accession>
    <name>A0A3B4BVT1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CERT1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVT1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008168.2">
        <property type="protein sequence ID" value="ENSPNAP00000003757.2"/>
        <property type="gene ID" value="ENSPNAG00000010522.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271828"/>
    <dbReference type="OMA" id="NSWIMES"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07698">
        <property type="entry name" value="IgC1_MHC_I_alpha3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000001">
        <property type="entry name" value="H-2 class I histocompatibility antigen, alpha chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="241"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="537"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="339"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003757.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="673" mass="77090" checksum="713891ABBF623065" modified="2024-11-27" version="2">MCGWISKNSRISSCCFWWGEELDGSAFSFSVCIELHSGGTKIMKSLLFLALTINLASAASHSLHYIYTAVTPGMQFPEFTTVGVVDREQCDYYDSNISRVIPKAEWMKKIEADDPEYWNRNTQTLLSDQETFKANLNTLMQRFNHSDGFHTFQWMYGCELHDDGTRRGYMQYGYDGEDFISLDLNTETWIAANQKAVITKYNWEHVNWAAQVKTYLENSCTEWLNKYVNYGRSTLERKVSPEVSLFQKDSSSPVVCLATSFFPKAVMISWQKDGEDLDEDVELRETLPNQDGTFQKRSVLTVSPEELKKNQYSCVIQHSGLEKEMVLQMADRRVLSVEPSGSSKSGPTKSTPQSASHSLHYIYTAVTPGMQFPEFTTVGVVDREQCDYYDSNISRVIPKAEWMKKIEADDPEYWNRNTQTLLSDQETFKANLNTLMQRFNHSDGFHTFQWMYGCELHDDGTRRGYMQYGYDGEDFISLDLNTETWIAANQKAVITKYKWEHVNWAAQVKTYLENSCTEWLNKYVNYGRSTLERKVSPEVSLFQKDSSSPVVCLATSFFPKAVMISWQKDGEDLDEDVELRETLPNQDGTFQKRSVLTVSPEELKKNQYSCVIQHSGLEKEMVLQMADRRVLSVEPSGSSKSGPTKSTPQSGESDLFVSLNVFEIIKVFSMSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWL5</accession>
    <name>A0A3B4BWL5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Collagen, type IV, alpha 5 (Alport syndrome)</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWL5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004072"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007636.2">
        <property type="protein sequence ID" value="ENSPNAP00000004072.2"/>
        <property type="gene ID" value="ENSPNAG00000010600.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157678"/>
    <dbReference type="OMA" id="TCSNNES"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.240.10:FF:000001">
        <property type="entry name" value="Collagen IV alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.240.10">
        <property type="entry name" value="Collagen IV, non-collagenous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001442">
        <property type="entry name" value="Collagen_IV_NC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036954">
        <property type="entry name" value="Collagen_IV_NC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF903">
        <property type="entry name" value="COLLAGEN ALPHA-1(VIII) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01413">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00111">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51403">
        <property type="entry name" value="NC1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Collagen IV NC1" evidence="10">
        <location>
            <begin position="334"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="21"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004072.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="559" mass="56033" checksum="2947262AAA50B818" modified="2024-11-27" version="2">MCLLIEYYFFVCTGNPGPTGQPGFPGLPGPKGEPEPGFGIPGPQGPTGLKGFKGSSGPKGDSGFPGSTGGPGRPGSKGDMGVMGAPGPAGNPGNPGVPGFPGPKGDDGFQGQPGSPGIPGTKGDKGDAGIPGPPGFISPENLPKGAKGEPGPPGDPGPAGLDGRPGLPGPPGPKGDPGLSGAHGAPGTPGLKGIMGEMGLPGPPGAKGSPGLPGQPGQPGFSGAPGFSGPKGEPGTPGFGQPGLPGPKGDPGSPGFPGSPGPKGLQGPPGLPGLPGSPGSKGDLGLPGLQGESGAPGSLGFPGEKGQAGRDGIPGPAGVKGDPGAPGLVSAAHGFLITRHSQSVDLPRCPDGTTPIYNGYSLLYVQGNERAHGQDLGTAGSCLKRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPIKGESIKPFISRCTVCEAPAMVIAVHSQTIQVPECPENWIDLWIGYSFMMHTSAGAEGSGQALASPGSCLEDFRSSPFIECHGHGTCNYYGNSYSFWLATVDKNEMFRKPQSETLKAGYLQTRVSRCIVCMKRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWN7</accession>
    <name>A0A3B4BWN7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">OSBP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWN7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004097"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007591.2">
        <property type="protein sequence ID" value="ENSPNAP00000004097.2"/>
        <property type="gene ID" value="ENSPNAG00000010662.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157987"/>
    <dbReference type="OMA" id="LPEMKGW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13284">
        <property type="entry name" value="PH_OSBP_ORP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000074">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000003">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF194">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="22"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="250"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="666"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="666"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004097.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="750" mass="85822" checksum="F4661D111C88DC7E" modified="2024-11-27" version="2">MKTSSASASSAATTTATPAPGSDTYKGWLFKWTNYLKGYQRRWFVLSNGLLSYYRTQAEMAHTCRGTINLATAHIDTEDACNIVLSSGGRTYHLKASTEVERQKWVTALELAKAKAIRMMNDQSGEEEPTSRSDRSELQGALKTLASKLDDLCTCNELIAKHGAALQRSLSELEALRVPLEGSEKIKAVNERATLFRITSNAMINACRDFLELAESHSRRWQRVLQHEREQRTHLEETIEQLAKQHNSLERAWREAPTHSTNTPDTPTTHTGELSQPRKEEASDEDEDTEYFDAMEDSPAFITVTAADPSQHRLESLTHTHTHTHLLYNDLSGREVQPRRKRRTRIPDKPNYSLNLWSIMKNCIGKELSKIPMPVNFNEPLSMLQRLTEDLEYSELLDKAARCENSLEQMCLVAAFSVSSYSTTVHRTAKPFNPLLGETYELDRLEEFGYRSLCEQVSHHPPAAAHHVMSQRGWTLWQEITIASKFRGKYLSIMPLGAIHLQFHASGNHYVWRKVTSTVHNIIVGKLWIDQSGDIDIVNHRTKETCQLKFSPYSYFSREVPRKVTGVVADSEGQAHYILSGTWDERMESAKIVQSSRGSSGAEGKQKTVYQTLSPRLLWKKYPLPYNAENMYYFSSLALTLNEEEVSVSLTDSRLRPDQRLMEEGRWDEANTEKQRLEEKQRAVRRRREADASNAVDEGEEGEGKEYEGYQPLWFHQRMDPLTGETSFVYKGGYWEAKERRDWSMCPDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXR3</accession>
    <name>A0A3B4BXR3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Estrogen related receptor beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ESRRB</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the nuclear hormone receptor family. NR3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546327.1">
        <property type="nucleotide sequence ID" value="XM_017690838.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BXR3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008889.2">
        <property type="protein sequence ID" value="ENSPNAP00000003341.1"/>
        <property type="gene ID" value="ENSPNAG00000006181.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423503"/>
    <dbReference type="CTD" id="2103"/>
    <dbReference type="GeneTree" id="ENSGT00940000153433"/>
    <dbReference type="OrthoDB" id="5799427at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07170">
        <property type="entry name" value="NR_DBD_ERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06946">
        <property type="entry name" value="NR_LBD_ERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000009">
        <property type="entry name" value="estrogen-related receptor gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000008">
        <property type="entry name" value="estrogen-related receptor gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024178">
        <property type="entry name" value="Est_rcpt/est-rel_rcp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050200">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027289">
        <property type="entry name" value="Oest-rel_rcp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002527">
        <property type="entry name" value="ER-like_NR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500939">
        <property type="entry name" value="ERR1-2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="14">
        <location>
            <begin position="125"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="15">
        <location>
            <begin position="233"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR002527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="50864" checksum="B874C911E7142EA9" modified="2018-12-05" version="1">MDISELCIPDPLSYHNQLLNRMAAEERHLPSSCGSYIKTEPSSPSSLVDTASHPSPGAHSDASGGYASAINSHSNGLDSPPMFTAAGPLGAAGSACRKRYEDCSSTLLEDSGPIKCEYMLNSIPKRLCLVCGDIASGYHYGVASCEACKAFFKRTIQGNIEYSCPATNECEITKRRRKSCQACRFMKCLKVGMLKEGVRLDRVRGGRQKYKRRIDTESSAYLGLTLPPPAKKPLTKIVSHLLVAEPEKIYAMPDPTMPESDIKALTTLCDLADRELVVIIGWAKHIPGFSSLSLGDQMSLLQSAWMEILILSIVFRSLPYEDELVYAEDYVMDEEHSRLTGLLDLYVSILQLVRKYKKLKVEKEEFVTLKAIALANSDSMHIEDVEAVQKLQDALHEALQDYESGQHTEDPRRAGKLLMTLPLLRQTATKAVQHFYSIKVQGKVPMHKLFLEMLEAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0F3</accession>
    <name>A0A3B4C0F3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein disulfide-isomerase A6</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PDIA6</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May function as a chaperone that inhibits aggregation of misfolded proteins. Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling. May also regulate the UPR via the EIF2AK3 UPR sensor. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGGT1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with MICA on the surface of tumor cells, leading to MICA disulfide bond reduction which is required for its release from tumor cells. Interacts with ITGB3 following platelet stimulation. Interacts with ERN1; the interaction is direct. Interacts with EIF2AK3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0F3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004134"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007509.2">
        <property type="protein sequence ID" value="ENSPNAP00000004134.1"/>
        <property type="gene ID" value="ENSPNAG00000010615.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155646"/>
    <dbReference type="OMA" id="QCAAAHP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02983">
        <property type="entry name" value="P5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03001">
        <property type="entry name" value="PDI_a_P5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000032">
        <property type="entry name" value="Protein disulfide-isomerase A6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000050">
        <property type="entry name" value="protein disulfide-isomerase A6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057305">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24541">
        <property type="entry name" value="Thioredox_PDIA6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017427403" description="Protein disulfide-isomerase A6" evidence="16">
        <location>
            <begin position="22"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="17">
        <location>
            <begin position="10"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="17">
        <location>
            <begin position="158"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="416"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="444" mass="48364" checksum="B2CEF55DAFBFD716" modified="2018-12-05" version="1">MSSSSSLRSVVVSALILAVHGFYTASDDVVELNPSNFNKQVLQSDGLWLIEFYAPWCGHCQRLTPDWKKAATALKGIVKLGAVDADQHKSLASQYSIRGFPTIKIFGANKNNPEEYQGGRTSQAIVEGAMTALRSLVKDRLSGRAGGSGQSKQSGGGSGAGNKNDVVELTDDNFDRLVLNGDEVWMVEFFAPWCGHCQRLEPEWTAAATEVKEQTKGKVKLGAVDATVHQGLASRYGIRGFPTIKVFNKGENPEDYEGGRTRSDIVAHVMELFSANAPPPEVTEVLNQDVFKKTCDDHQLCIVAVLPHILDTGASGRNSYLDVMRMMAEKYKKKMWGWLWTEAGAQMELESSLGIGGFGYPAMAAINARKMKFSLLKGSFSETGIHEFLRDLSVGRGSTSPVGGGALPKIHTVEAWDGKDGQLPEEEDIDLSDVDLDDLEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0V7</accession>
    <name>A0A3B4C0V7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phospholipase A2, group IVC (cytosolic, calcium-independent)</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0V7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004461"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006961.2">
        <property type="protein sequence ID" value="ENSPNAP00000004461.2"/>
        <property type="gene ID" value="ENSPNAG00000005575.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234606"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF39">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2 3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLA2c" evidence="5">
        <location>
            <begin position="6"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004461.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="566" mass="64842" checksum="CBA46F0DA532C669" modified="2024-11-27" version="2">MDTSRTCESSEVRIDHSLSEVERQHLTRRTETVLDCLKQHGISCSEHDMPNIAVLGSGGGLRAMVGLLGSLSRLKKEGLLDCIMYLSGVSGSTWCMASLYNGPNWSTKLETVEDNIVQRLAEGRVSWSQMGQKLANYYANNENFSLTDVWAALIVSNMVNEIDEHKLTEQRANYTNDPYPIYTVIDKQCKYDTLNADVWFEITPDESGYSLSGAFVDSSCWGSQFKSGERIKDQPETDMLYLQGLCGSAIADMEENKKFLYEALKQLIEYIFGYTEERHTSQIPDVKKGQQALLALVELNLSVIRGKDPSIYLNIIEDLLKDKYDEIGRMTLRLKKMMTSEGKMTETELQNYNLRVCSFITDLFRGQGSWFDMWMAIVRSLILLVSWTWGTNYDFLYRMQVEVIDPSVLDSETRQYEDAGLLLNSPYFSVLRKERHIDLIISLDFSAGNPFETVVTTAKLCKHLQIPFPEVVVPAEDTEPQDFYVFRGYSKAPTVIHIPLFNAVNCKGEIKQWEKRYQTFQKSYTPEMITDLLEKAAENIKNNKEKLLKEIINIIQEKKTYKHGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C125</accession>
    <name>A0A3B4C125_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017579018.1">
        <property type="nucleotide sequence ID" value="XM_017723529.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C125"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006855.2">
        <property type="protein sequence ID" value="ENSPNAP00000004531.1"/>
        <property type="gene ID" value="ENSPNAG00000010972.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443104"/>
    <dbReference type="GeneTree" id="ENSGT01030000235435"/>
    <dbReference type="OMA" id="ISMERDH"/>
    <dbReference type="OrthoDB" id="8807033at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="24770" checksum="4D4CEE880EF65202" modified="2018-12-05" version="1">MKSKGVMMSWPDPHSILRHFVLLKYLQVMALPLMNGLLSGMGALASLYTALLLNSPRASRKASSVLLGQLAQADSLLLLHWGLGAVRPALGVPQEALEGLEHGLLASHRLASLLLLGCVSLEALLVCRRPAETRSLRTVHCARLASTTVWAAVALEFVVLRTSEYESALRDRGGFITLLALTCTFLAPFIQILSFCVTLMLWLANTWTCYTIFCYKPQRQTSCYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7C9</accession>
    <name>A0A3B4C7C9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C7C9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002906.2">
        <property type="protein sequence ID" value="ENSPNAP00000006801.2"/>
        <property type="gene ID" value="ENSPNAG00000012852.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156765"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001843">
        <property type="term" value="P:neural tube closure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000047">
        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF12">
        <property type="entry name" value="VASODILATOR-STIMULATED PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="WH1" evidence="11">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006801.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="127" mass="14399" checksum="2E345803A8F9ACF4" modified="2024-11-27" version="2">MCSENATELFDMCQARATVMMYDDAGKRWVPAGSGAQAVSRVHIYQNPNNNSFRVVGRKLQADQQVVINCPLMRGIKYNQATPNFHQWRDARQVWGLNFSSKDEAMQFANTMMYALDVLSGEKNNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDG1</accession>
    <name>A0A3B4CDG1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Junctional cadherin 5 associated a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017566469.1">
        <property type="nucleotide sequence ID" value="XM_017710980.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000008499"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014591.2">
        <property type="protein sequence ID" value="ENSPNAP00000008499.2"/>
        <property type="gene ID" value="ENSPNAG00000014111.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435259"/>
    <dbReference type="CTD" id="100001168"/>
    <dbReference type="GeneTree" id="ENSGT00940000166891"/>
    <dbReference type="OMA" id="AYSEERM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903589">
        <property type="term" value="P:positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028221">
        <property type="entry name" value="JCAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34757:SF2">
        <property type="entry name" value="JUNCTIONAL CADHERIN 5-ASSOCIATED A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34757">
        <property type="entry name" value="JUNCTIONAL PROTEIN ASSOCIATED WITH CORONARY ARTERY DISEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15351">
        <property type="entry name" value="JCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="555"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="631"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1362"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1460"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="720"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="748"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="776"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1371"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1389"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1401"/>
            <end position="1414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008499.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1484" mass="165786" checksum="A52CB4D8610537DA" modified="2024-11-27" version="2">MYSVEDLLISHGYKVSSSNNNVPPSHSPSSHHTTYDQRPPTRSSSRCEIGEKRTGHGTVNGYKGDCVYGGGGVRQMASRGGPSDTESRDRKQRTEEEDNGNLGDGQSPGDSLTTDSGFHESHRVMYSQPRPERDVSYWRRRGQDFNALLDYADFRELHGKESGVPIKPEGMQRRPESALSTEEHRRERQRRADSARARERELALYQWRIAAERKYQSLGTEEWRPAVGMGRQTSESESERWAQEQQRRPRTAEGAVPPRPKAKSQSLPRMAMPSDSVQFLSTSSPGQDPYGSYRTNGHQIRESHGRHVSEGESRERWIDNVRTGVQSAPPSKPRFSRPLRPPSYEVHQQMRGSSEMLSGEFMTHARDRTPLPFTRQEYFAQEPGGSGVEPPGYIPPPSYRRQPPIRGGHRTYANSMGNYQYRGDPYMQGPAMAEVQEWFIRQTGMSWPDHHRDGRRSMPCRRQAYPAYSEERMGNVQYIPFDDPRVRHISGGGIDGNSLTDADKIRNIRKEIPIVSLSEKAPDDSAFPLTEKIYSSTEASKSNISDCINEAAMQKDVSKDSKSVSDENSNRYLSGHDYSDSFQMTPSQQSCETITQVKQFEAQTAAENKKNKKKLKETMFCLVSVPINVMASKGTADPNNNEKVPSPVVTPSENTVTQPHSQDSSTISISSESHIQPSSASAIKNSKRAPLKKEIVDVWSLQASADKELCYAGSWPGDQYRNQETQTGSPEVSRGVYSPSSHTQATYDPPSSDTTTDSGVSTDCNATFGYPMKGQKNLNLSSNSAFSRTRAGSSSSAKSPAQLLTSPTPPSPDHQPPMRKSAPKSRAPGGQEIFGQFLLKPVNRRPWDAIGELESFNKELQDQNSKQQPSIDQTLEDLDEALQNILEVTNTCTEVMQPGKTPKNLKRYLSHKEQSPEQLLKKDNLNIKSDLENKGKTEVDQEYREVRSAFSRPKERVANSDIAQRQDMPVQNDTSFVSYEVLQRIQGEPRGQRLDIPVPKESLLKDVGLTVYTAIPDTVTSGSPTGLSPDSPMLTSPSDNSETCEALHITSSDSGGDRSRNSPEFIVDQSLNIQTCITAKLDLEKEVESRSKGVTKQRSPHIHGARTQHFRFKGNYADDDDDGYSDYLSWSNEKTLADEHLESLLSQEKANSLPTEDLSKLYQVQCAQGIPEHESIEQRAARILGIAVPAEALVVNQNDGKQNEGKAKVMTQAGFAMQTREISLNEDTQYVSREYEEVVETSVHSCELKMVKKDPGIRQDNASREELSTESQTVSAVLELPEFPPSNLRLSLPVTEDEELALSVCGGEKKASLAEEVSEGQEDKSSAHLVSYMFRNEELSAEHINPVEEGEPISCISTFTKEMEKEEEMGEEKSVQEEEVLEGEKEQTVESEMEADECEAAFEKKEDEDDRKSEEPEEEMFQESSQQQPKFILCPVPQPRSGTVTKREITLPATFSMSDDLAALDDDDVHSVSDAYDPSRVERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDK2</accession>
    <name>A0A3B4CDK2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551004.1">
        <property type="nucleotide sequence ID" value="XM_017695515.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CDK2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008951"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035127.2">
        <property type="protein sequence ID" value="ENSPNAP00000008951.1"/>
        <property type="gene ID" value="ENSPNAG00000014527.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426198"/>
    <dbReference type="CTD" id="450035"/>
    <dbReference type="GeneTree" id="ENSGT00940000160420"/>
    <dbReference type="OMA" id="CPIVTKQ"/>
    <dbReference type="OrthoDB" id="5842031at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046661">
        <property type="term" value="P:male sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14419">
        <property type="entry name" value="CUE_DMA_DMRTA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005173">
        <property type="entry name" value="DMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF120">
        <property type="entry name" value="DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03474">
        <property type="entry name" value="DMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="10">
        <location>
            <begin position="28"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="8">
        <location>
            <begin position="28"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="436" mass="47396" checksum="BEF71F7401B6FCFE" modified="2018-12-05" version="1">MNGYGSPYLYVGGPVAQARAPLQRTPKCARCRNHGVLSWLKGHKRYCRFKDCTCDKCILIIERQRVMAAQVALRRQQASESLESLIPEALRTPGAELRWSGEGLSTQPGPELNEDLSGEQSGGENSGSTSDREQETASSPEEPKPNPCYTPNTPEPPSHPEEPRYSASKPCTDPEPKPDSPKYPSEPNLLIEGLSGSVSLPFNLRANRPPLEVLKKIFPGHKPAVLELILRGCGGDLVGAIEILLSSRSSAKVDKPSPDGSEALVLPSNGHLFEHPLSSYPVSSTKWSVGSAFRVPDSLRFSPESPTGGMVPGPLGVPLQHGFPQPPRYPLMLRNSLSRGQAGGPFVHSDVTLWNTMTLQQQYQLRSQYVSPFSGPSPGAFRSSPILTSRAAQEEQRISVQEDGCPVTAKQTIYAADEEYDERSDSSDSRILNTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGG8</accession>
    <name>A0A3B4CGG8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGG8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015970.2">
        <property type="protein sequence ID" value="ENSPNAP00000009554.2"/>
        <property type="gene ID" value="ENSPNAG00000015067.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157340"/>
    <dbReference type="OMA" id="MTYSNFR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900026">
        <property type="term" value="P:positive regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052223">
        <property type="entry name" value="Actin_Cytoskeleton_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271:SF14">
        <property type="entry name" value="TRIO AND F-ACTIN-BINDING PROTEIN-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009554.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="50667" checksum="94863A0CAE750352" modified="2024-11-27" version="2">MKFYEIDFTRDEPDGEIDVRRCVRVVEFEADKNYGLQIHTRDGEVTLSAMTSRIRRNWIDILQRHIRPNGLPDITLISNVDSSDLWTLFHFNFSNGLYLFFRHMNSDKDTSSSHPDQHNSGSEVMSPVQDEPDMMSSPSTNQREAGVGRDKDQEKRLEDRTKWFQEGLLDREGESPWDKVQLKKGTVASVTHMMPQISETLIGTDIEKKWEDFEKIPFGEMMLISSNGSQVLSLRQQVEALRKGRIGGGPIVPCGPDTPCASKLEQIEREHRERLQEMEREHERERREMEREKERLLKEEAQNAIQDVQIFRCVCAFRPESVSLQQELEGLSERYSQRCVELRRVQHTAGQKHTLIQEKEREVLQLRKENQDLQARLTEEMSLMRSFITGQKSGMLLRVKENEIEYLHKEIGCLRNEIQSLTKVTLDHVLFILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIG8</accession>
    <name>A0A3B4CIG8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CCDC39</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC40 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC39 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570121.2">
        <property type="nucleotide sequence ID" value="XM_017714632.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIG8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011218"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018105.2">
        <property type="protein sequence ID" value="ENSPNAP00000011218.2"/>
        <property type="gene ID" value="ENSPNAG00000016861.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437501"/>
    <dbReference type="GeneTree" id="ENSGT00390000015010"/>
    <dbReference type="OMA" id="NSKNCDE"/>
    <dbReference type="OrthoDB" id="10259720at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033290">
        <property type="entry name" value="CCDC39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18962">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18962:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24161">
        <property type="entry name" value="CCDC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="867"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="923"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="24"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="178"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="269"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="458"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="664"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="733"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="803"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011218.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="955" mass="110037" checksum="35AE6B9A714752AA" modified="2024-11-27" version="2">MASAVLSAIDWDEGFTIPVPNAANKALEEKLQRKQKEKASIDNKLSENKDRINAMTEHLKNVQLELSHTQALIRAREKETESELHFKALAEREMGRLRQVIAQLENELGSLREKKNSQENNIFKATQKLEELKSQLNWDQQTLDAWLEESARKDEDTMAIIKYAQQDESRIREVTLSIEKLTLEANRKKKALDNELTETITAQIGLDKTAETFRQAHMERQELIRQWENTIEQMRRRDQEMQQCALTLAEKNQMIREREALIKEKKSFLTSEVENNKECERKIAAAERQATRLRQQLQEEEGNRIRLQDELETLKGTVDGAATDVEVLRSNLTSMKKEINDKTIKLEEARLQNVALGEKLQVATEAALSVEERAEQMEQMLCDQELHSKEIDAQLQRQRELLFRKRQELQTLRAKEKNATAELSGSRAALSNLDSRLNKLDQHALKQQEIICNQDVQIQMLKMKMARLKGEVNTEEKQVLEKRTSELNKALEEKKKTAIMLTSQLKKLQDDIRCLRKETEKTGAEKRDLTTKIEELRLFNDTSDKELKKLRLKKQDSMVELNILRLEVKRLRDLLYNKADGVLSLEKRRLQLQAAMKERQEEIRVHRETLTKQLKITDQERQRLSAEVHERLSKIDKMRKRYEIMTIAMAAPEGEEDKSQAYYIIKAAQEKEELQREGDELDTKIRKTEKEVKALENTLHVVNNWNSTYRKSFSKATESSPEYQEKMKLDEQKRAAEDKYKYKRRQIRELQEDIEGMNSSLNSLQQEEAAQSKRNGDVQTRILSLNKELGSQQEKLGRVTKQCSRLTKEIRSAKKTKEETFEERDIELKELKDLNKTVNKMLLEVIEEHPDLQATLQMYFMQASLALPSPASTPSSRQSSKISSARSSISLRSSGSSAGSSSRGSAVQSRTVKTVELGLGLSVSSPLASSTSPRASCSSSSSSSGRSRSSQLKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIZ1</accession>
    <name>A0A3B4CIZ1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pentraxin (PTX) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540412.1">
        <property type="nucleotide sequence ID" value="XM_017684923.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIZ1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011378"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018342.2">
        <property type="protein sequence ID" value="ENSPNAP00000011378.1"/>
        <property type="gene ID" value="ENSPNAG00000017039.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412755"/>
    <dbReference type="CTD" id="553230"/>
    <dbReference type="GeneTree" id="ENSGT01060000248591"/>
    <dbReference type="OMA" id="AWKNEVG"/>
    <dbReference type="OrthoDB" id="8871962at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051966">
        <property type="term" value="P:regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00152">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000012">
        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF1">
        <property type="entry name" value="NEURONAL PENTRAXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017480121" description="Pentraxin (PTX) domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="10">
        <location>
            <begin position="234"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="134"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="442" mass="49148" checksum="8BA439FA4ED26F58" modified="2018-12-05" version="1">MITLAVGLLYVYALGSGRVARASAQAKGSRFVCTAVPPGADLSCPVETTNRVQSSSPQEDEFRSTIIQLRETVLQQKETIVSQQGTIKELNAKLSRCEAAAQDARDSKARGGARRKDYGKNTMGDLPRDPTETIEQLGKTMQSLKDRLENLEQQQLRANVSGAAFPNELRDLLKRRLGDLESQLLRRVAELEEEKSQLYNETAAHRQRTENTLNSLLERITELERSNSAFKSPEDFKISLPLRTNYLYGRIKKSLPEMYAFTVCMWLKSSASPGIGTPFSYGVPGQANEIVLIEWGNNPIELLVNDKVAQLPLSVSDGRWHHICITWTTRDGFWEAYQDGERLGTGENLAPWHPIKPGGVIILGQEQDIVGGRFDATQAFVGELSHFNMWDRVLRPIDISGMANCSAYMPGNVVPWIDGNVEVFGGATKAALEICEDRLFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CKC8</accession>
    <name>A0A3B4CKC8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RNH1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CKC8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018893.2">
        <property type="protein sequence ID" value="ENSPNAP00000011815.2"/>
        <property type="gene ID" value="ENSPNAG00000035969.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287004"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000538">
        <property type="entry name" value="Si:ch211-127b6.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011815.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="393" mass="43559" checksum="38A067DCBC924A6D" modified="2024-11-27" version="2">MGLLKPEEEQRNDSSVTTALLNHLRKVPLQELREKLQRLLESRSGVLLCGEDLTEKSCAVLASALSSKNSNLTELDLSLNELQKSGVKKLCEGLRSENCKLETLTLAYCRIRDEGCADLIKALKSNPQSKLTELNLRGNDPEEVAVELFRDPRRILEKLQLRGEGLTEKSCAVLASALSSQNSKLTELDLSYNKLQDSGVKKICEGLRSPNCKLETLRLSACRLTEESCADLASALMVKKSPLRELNLSRNELQDSGVKKLCDGLRSENCKLETLNLSACRLTEESWADLASALMVKNSPLRELDLSKNTLQDSGVKKLCDGLRSENCKLETLKLSSCSITDEGCAELVTVLKSNPSYLTELDLTANQLEDSGVEQLIELKDNPDYKLEKLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CL15</accession>
    <name>A0A3B4CL15_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Stress-associated endoplasmic reticulum protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SERP2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Interacts with target proteins during translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation and facilitate correct glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SEC61B, SEC61A1 and the SEC61 complex. Interacts with CANX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the RAMP4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566273.1">
        <property type="nucleotide sequence ID" value="XM_017710784.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CL15"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011531"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018522.2">
        <property type="protein sequence ID" value="ENSPNAP00000011531.1"/>
        <property type="gene ID" value="ENSPNAG00000006130.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435163"/>
    <dbReference type="CTD" id="387923"/>
    <dbReference type="GeneTree" id="ENSGT00940000162467"/>
    <dbReference type="OMA" id="ATKFITQ"/>
    <dbReference type="OrthoDB" id="16679at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010580">
        <property type="entry name" value="ER_stress-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15601">
        <property type="entry name" value="STRESS ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN SERP1/RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15601:SF20">
        <property type="entry name" value="STRESS-ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06624">
        <property type="entry name" value="RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="65" mass="7413" checksum="4A689CEF04336C06" modified="2018-12-05" version="1">MVAKQRIRMANEKHSKNITQRGNVAKTLRPQEEKYPVGPWLLALFVFVVCGSAIFQIIQSIRMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CL99</accession>
    <name>A0A3B4CL99_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="15">PARP</shortName>
            <ecNumber evidence="15">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PARP16</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Intracellular mono-ADP-ribosyltransferase that plays a role in different processes, such as protein translation and unfolded protein response (UPR), through the mono-ADP-ribosylation of proteins involved in those processes. Acts as an inhibitor of protein translation by catalyzing mono-ADP-ribosylation of ribosomal subunits, such as RPL14 and RPS6, thereby inhibiting polysome assembly and mRNA loading. Mono-ADP-ribosylation of ribosomal subunits is promoted by NMNAT2. Involved in the unfolded protein response (UPR) by ADP-ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors. May also mediate mono-ADP-ribosylation of karyopherin KPNB1 a nuclear import factor. May not modify proteins on arginine or cysteine residues compared to other mono-ADP-ribosyltransferases.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with KPNB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4CL99"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011319"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018259.2">
        <property type="protein sequence ID" value="ENSPNAP00000011319.1"/>
        <property type="gene ID" value="ENSPNAG00000016973.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183129"/>
    <dbReference type="OMA" id="LLYGQSC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000005">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051838">
        <property type="entry name" value="ARTD_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041400">
        <property type="entry name" value="PARP16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21328">
        <property type="entry name" value="POLY ADP-RIBOSE POLYMERASE FAMILY, MEMBER PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18084">
        <property type="entry name" value="ARTD15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="17">
        <location>
            <begin position="123"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="364" mass="41762" checksum="707F0BC0D1B1E217" modified="2018-12-05" version="1">MDCKVPVIIAAYAPHSSRCYTDLYCYFLSAGVAVVGMQPPLPPAPVRELVRSCLHLDPVAADLRCSLFVAAVQNYKRDSVLRPFPSRYLRGHVKDFEELQKDVSSLPSVRDLVRLGLGEGDHHLALMHWVLSSKSFSVKTIQKEEWARLSQLIQNEGLCSPVPDFLFELEYCEQMNSKFEKTRAGRDILYAFHGSRLENFHSIIHNGLHCHLNKTSLFGEGTYLTSDLSMAILYSPHGNGWRESVLGPLLSCIALCEIIDHPDVKCQVKKKDSEGLDRQRLRARNSEGGDVPEKYFVVTNNELLRVKYLLVYSQKRYRTRHTQRTSWLVRHHFAIMMGLYLLLLIFIGAFNSTSFQSFWHRIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMJ3</accession>
    <name>A0A3B4CMJ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone H1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMJ3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012573"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019905.2">
        <property type="protein sequence ID" value="ENSPNAP00000012573.2"/>
        <property type="gene ID" value="ENSPNAG00000030480.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183089"/>
    <dbReference type="OMA" id="CEEIRVI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000075">
        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF20">
        <property type="entry name" value="H15 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="11">
        <location>
            <begin position="27"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012573.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="113" mass="11364" checksum="7D718C51D287EE75" modified="2024-11-27" version="2">MAEAAPAPAAAPPKAPKKKSAARPKKAGPSVGELIVKAVSQSKERSGVSLAALKKALAAGGYDVDKNNSRVKIAVKSLVTKGTLVQTKGTGASGSFKLNKKQAEARRGNVGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNV6</accession>
    <name>A0A3B4CNV6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GMP phosphodiesterase delta subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDE6D/unc-119 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNV6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013048"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020581.2">
        <property type="protein sequence ID" value="ENSPNAP00000013048.1"/>
        <property type="gene ID" value="ENSPNAG00000018885.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014595"/>
    <dbReference type="OMA" id="MEEWNGF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001287">
        <property type="term" value="P:negative regulation of caveolin-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900186">
        <property type="term" value="P:negative regulation of clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071632">
        <property type="term" value="P:optomotor response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048841">
        <property type="term" value="P:regulation of axon extension involved in axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.40:FF:000004">
        <property type="entry name" value="protein unc-119 homolog A isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.40">
        <property type="entry name" value="GMP phosphodiesterase, delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051519">
        <property type="entry name" value="PDE6D_unc-119_myristoyl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037036">
        <property type="entry name" value="PDED_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951:SF6">
        <property type="entry name" value="PROTEIN UNC-119 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951">
        <property type="entry name" value="RETINAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="6">
        <location>
            <begin position="85"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="246" mass="28726" checksum="020DFF62E1BA90E9" modified="2018-12-05" version="1">MSSEREEESGEERGEENVDGEGEGEEENEGEVRDMEEWNGFLLEGTTEKGQEDDVLVEWKPGDPVTPQYVLRLAGYTEDYLCSPEDNIYNISFSRFKIRDLEGGSVILDLKRHCPTEIKDVNEQEAARFIQYHFTPAFLNLREIGATLEFTVGSKAVNRFRLIERHYFRDMLLKTFDFEIGFCIPHSRNTCEHIYSLPDLDPRTIEEMISHPFQTRSDSFYFANNKLIMHHKAEYSFCKGMEMDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQL3</accession>
    <name>A0A3B4CQL3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a hydroperoxide + L-cysteinyl-[protein] = S-hydroxy-L-cysteinyl-[protein] + an alcohol</text>
            <dbReference type="Rhea" id="RHEA:67124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:17193"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:67125"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sestrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017562551.1">
        <property type="nucleotide sequence ID" value="XM_017707062.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQL3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014357"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022225.2">
        <property type="protein sequence ID" value="ENSPNAP00000014357.1"/>
        <property type="gene ID" value="ENSPNAG00000020253.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432922"/>
    <dbReference type="CTD" id="100149048"/>
    <dbReference type="GeneTree" id="ENSGT00950000183168"/>
    <dbReference type="OMA" id="ILAHCHA"/>
    <dbReference type="OrthoDB" id="337464at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070728">
        <property type="term" value="F:L-leucine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071233">
        <property type="term" value="P:cellular response to L-leucine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990253">
        <property type="term" value="P:cellular response to leucine starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901031">
        <property type="term" value="P:regulation of response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1290.10:FF:000001">
        <property type="entry name" value="Sestrin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006730">
        <property type="entry name" value="Sestrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474">
        <property type="entry name" value="P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474:SF1">
        <property type="entry name" value="SESTRIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04636">
        <property type="entry name" value="PA26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="439" mass="50135" checksum="7EFAB8F270469663" modified="2018-12-05" version="1">MIICAKKMDYHLGTPCQLVKNQVMKVNSEKERASPLCMKALASRGRLDTVSQQMASHPQYLESFLRTQHYILYMDGPLPHHYRHYIAIMAAARHHCSYLVSLHSAQFLRVGGDPLWLQGLEAAPPRLQQLDHINKVLAHQPWLTAHSHIQALLKPGEQCWSLAELVQAVVLLAHCHSLCSFIFGSASETDTAPTLRVPHGTPPGYCLCDAANGNTALPPTERTPRRRSLDSSCELTCLRERIQKSQEERKGDPILHSHTLLHIDAEEEEEEMMCSTDPSRFVTDPDFGYQEFARREEDHFQVFRVQDYSWEDHGFSLVNRLYSDIGHLLDDRFRSVASLPSPHDPDLKRAIWNYIHCIYGIRYDDYDYGEVNQLLERGLKLYIKAVACYPDSTKNLLCPLPLTPLKASEKVHVNLLVMEARLQAELLYALRAITQYMIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRT1</accession>
    <name>A0A3B4CRT1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CRT1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014053"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037189.2">
        <property type="protein sequence ID" value="ENSPNAP00000014053.2"/>
        <property type="gene ID" value="ENSPNAG00000032581.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162888"/>
    <dbReference type="OMA" id="EVHNERQ"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000006">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000013">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000007">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF44">
        <property type="entry name" value="MYOSIN HEAVY CHAIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="7" id="KW-0518">Myosin</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin tail" evidence="12">
        <location>
            <begin position="14"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014053.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="984" mass="113320" checksum="7FCD5DB785045AAC" modified="2024-11-27" version="2">NVSSNSGDNDSSIKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENEKQQSEEKIKKKDFEISQLLSRIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEFKAKNDEHVRQLNDLSAQRARLQTENGEFTRQLEEKEALVSQLTRGKQAYTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRSKYETDAIQRTEELEEAKKKLAQRLQDAEESIEAVNSKCASLEKTKQRLHGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEEALDQLETLKRENKNLQQEISDLTEQIGETGKTIHELEKAKKTVETEKSEIQTALEEAEGTLEHEESKILRIQLELTQVKSEIDRKLAEKDEEMEQIKRNSQRVIESMQSTLDSEIRSRNDALRVKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQGQLKDAQLHLDEAIRGQEEMKEQFAMVERRNNLMLAEIEELRAALEQTERGRKVAEQELVDVSERVNTSLINTKKKLEADLVQIQGEVDDTVQEARNAEEKAKKAITDVSAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAESLAMKGGKKQLQKLESRVRELEAEVEGEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKSYKRQAEEAEEQANTHLSRFRKVQHELEEAQERADIAESQVNKLRARSRDAGKVKSTYSNKHGNSIKLKETIKCLSFFILSNRDSSSNVPGLHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTI5</accession>
    <name>A0A3B4CTI5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">POLRMT</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Has DNA primase activity. Catalyzes the synthesis of short RNA primers that are necessary for the initiation of lagging-strand DNA synthesis from the origin of light-strand DNA replication (OriL).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner. Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM. Interacts with MTRES1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017556883.2">
        <property type="nucleotide sequence ID" value="XM_017701394.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000013839"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021575.2">
        <property type="protein sequence ID" value="ENSPNAP00000013839.2"/>
        <property type="gene ID" value="ENSPNAG00000019790.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429560"/>
    <dbReference type="GeneTree" id="ENSGT00390000008060"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1320.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000031">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="12">
        <location>
            <begin position="345"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="487"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="116"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="823"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013839.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1328" mass="152301" checksum="18D01EBCE33381D4" modified="2024-11-27" version="2">MRLLLNSQRSVKMSLLRLCSFPKCLAGRLVAERNVRHLLNVEHCLLCLRNRSHYERGIRDNPWTSQRRQYSAIPKKDDGNKRMWEQSHLLDVLEARIQQLQSDVLFDVKHSKVEFLKMPQRDKGPSPSKSQKTRPGEKTELPKAGPSNKKGATTDNPSTKTHTSRWFEKLNREKWNRTSKQSSLQQNVPDKLAKSSKGKSNKKQQTMKSSKGSMMLPGSSMKSISAVKRSKPVTKQEHAKPAASPVSHSMPKASESPVNEAIPVPEELSRDAELAEVEELEQMIKQWSSTGTEKVIEVKEVNRDADTQLNVCCHLEVCVFMGDLERAQRYLLFHHQNLFKRKTLTVSSYNIMMRMWAKKGSLHHIRQLFFLVEEAGLKPNVASYCAALECMGRNPESSSRVISRCLQQMEEDGLSVDDVFRQCVFRQDERDMVLKAVRTVRPDYEPSVTTENTLCSLPLVKDYYTQREDVRYPKLDFSLADLQDRFKRQLALEQANTITIDSVETMKPVKENTIRMRKLLAEQRAHWQKNLLRALRDSKLILGKSKLRNWKTDIYPFLCILRDEDYVNIMLQGLAKLPPSGDSLLNMARELGTAVHNIFCIQQKSHSQVVEKLACIYNSYTELLAKDTKSSSVLPREQWNRLEAERSAGPSLLSDDMSWKHVLIIQLGSFLVDLLVHELKIQNNILNPAQDKKLIPALYHMYTFRSNRQIGFIKPHPILTQIQLEAMETKLTFDSYVMPMLCPPVPWTSPKSGAYLLTPTKLMRTVEGAIQHQHLLEKCRNEDLHAVLDSLNQLSSCAWKINKPLLDIIISIFNDKGSEKLDVPPPVSEAPEIPRFNPHDPSYTPQEKAHMKREVLKAKKKFGEMHSLRMDALYKLSIANHVRDEVFWFPHNMDFRGRTYPCPPYFNHLGSDVTRAILLFAEGKPLGPKGLDWLKIHLVNLTGLKKRSSLAGRLEYANSIIEDILDSADHPLTGRKWWMDADEPWQTLACCMEIANASRSPDHTKFISHFPVHQDGSCNGLQHYAALGRDVIGATSVNLMPCELPQDVYSAVAQQVEEFRVRDAAKGLKIAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLKEIDDFPKEHIWDASHYLVHQVFSSLKEMFTGTREIQDWLTESARLIAKSGSTVEWVTPLGLPIVQPYHRARSHVLKSNIQFLSLQINYDIHERPDTVKQKNAFPPNFIHSLDSTHMMLTALYCYSAGITFVSVHDCFWTHALTVDTMNKVCREQFVALHSQPILQDLSKFMLQKYCSGPVGERQNKKFLEYWKMMQLLAKVPQTGDFDLHRVKESTYFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVE6</accession>
    <name>A0A3B4CVE6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">SVEP1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017574246.1">
        <property type="nucleotide sequence ID" value="XM_017718757.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000015318"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023484.2">
        <property type="protein sequence ID" value="ENSPNAP00000015318.2"/>
        <property type="gene ID" value="ENSPNAG00000021584.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009913">
        <property type="term" value="P:epidermal cell differentiation"/>
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        <property type="term" value="P:epithelial cell-cell adhesion"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0036303">
        <property type="term" value="P:lymph vessel morphogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="neurogenic locus notch homolog protein 3"/>
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        <property type="entry name" value="neuronal pentraxin receptor"/>
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        <property type="entry name" value="Protein crumbs 1"/>
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    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
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    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="9 20" id="KW-0732">Signal</keyword>
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    </feature>
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    </feature>
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        </location>
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        </location>
    </feature>
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            <begin position="1383"/>
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        </location>
    </feature>
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            <begin position="1424"/>
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        </location>
    </feature>
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        <location>
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    </feature>
    <feature type="domain" description="Sushi" evidence="24">
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    </feature>
    <feature type="domain" description="Sushi" evidence="24">
        <location>
            <begin position="2694"/>
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    </feature>
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    </feature>
    <feature type="domain" description="Sushi" evidence="24">
        <location>
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            <dbReference type="Proteomes" id="UP001501920"/>
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    <sequence length="3614" mass="392209" checksum="C898E933350CB389" modified="2024-11-27" version="2">MATLRLSMTLWLSLVFWTLLWSRMATSLPSQAQQPKPPQARRLSSQIQESAESKVERLGQTFRRNIRLLRERGGRLDLIFLLDESSSVGAANFGSELHFVRKLLSDFPVAPEATRVALVTFSSKSHVVTRVDYVSAPKSHQHKCSLFSKEIPAITYRGGGTYTRGAFQRAAQILRNSRQNSTKVIFLITDGYSNGGDPRPVAAALRNNGVEIFTLGIWQGNIRELHDMASAPKDQHCYLVHNFTEFEALARRALHEDLPTGSYIQEDMSHCSSLCVEGKDCCDLMASCKCGTHTGQYDCVCEKGHYGKGLQHECTACPPGTYKPEAAPGGIGTCLPCPKPQHTSRPGSTDISDCVCKDGYTLHNNTCQAVMCEALSPPENGFFIQNVCNNQYNSACGVRCMPGYELQGNSIRLCQADGTWSGAPPICTVRSCPVLSPPQHGALNCSEGGSPYSLECTVRCQQGYRLEGRAKLTCLSNSQWSGPPPRCVEVRCPPIISPKHMRVSPPACGERAMRSGATCRLTCRRGYRLQGNAEARCLLSGEWSHNLLKSACADAEPPWIQCPGNVIVDTDEHQGSSNVTLSAPVVGDNSGDQVIVHVTPILNPAQPFPIGTELITYTATDHAGNKANCSFTVTVIDMEPPLIDRCRSPPTVEATDTETPVYWEEPQFSDNSGAHLNISSTHSSGDAFPVGETTVFYTATDSSGNNRTCELIVTVQGSECEQPYVPVNADFSCVKEERGVNCTLICREDYSLTLNAVHSYFCPNNGRWEPPRSPERPDCSLNRVANNGFKPFEMLFKASRCDDPDLLKSFTGDFTTILGGLVPNICSGDDINCKLEVMSQGQCLEYNYDYPNGFAIGPGGWGSNWASQNGQDYAYFDGGFSPDRQHTPLQQDDAFQHSRSKRHRKISRPTRDQKIQIYFNITASIPLPLSRNDSVEVANQKRLLRTLEQLTNRLKRTLSKQHLSTFHVSSEMIVADPKSLESKRATLYCRPGSVLKGRMCVQCPVGTYFSVEYAECESCWRGSYQDEEGQTECKPCPDGYSTPYLHSRSVAECKAQCKPGSSSLSGLETCESCPLGEYQPGYGSRSCLHCPPNTSTVNRGAVDSTECGVPCSAGHFSRAGLVPCYPCPRDYYQPEEGRSYCLSCPFYGTTTVTGAKSIQQCSSFGSSFLPKEESVTTAPEVVVRKDYQASSQMFHECFLNPCQNQGTCEEVGVGYVCTCLPGFTGAKCESDIDECDSAPCQNGGLCRDGIGGFQCQCQPGFVGLLCEAEVNECSSSPCLNDGVCVDEVNQFTCSCTDGFTGPRCELEIDECESSPCQNGGVCKDLEAGYSCSCSQGFTGGSCEVNLDECFSTPCLNGGTCVDGVNDFRCECVKGYQGKLCQVDEDECQPNPCVNGATCEDGVGSYTCRCPPGFNGTRCETEMSSSFDLEFEVSGIHGYVMMDGLMPALTQITCTFWMKSSDTFNYGTPISYAVEGGSDNAFLLIDYNGWVLYVNGRERITDCPAVNDGRWHHIGVTWRSRDGDWRVYIDGSPSDGGKGLSIGNKIPGGGALVLGQDQDQRGEGFNPVESFVGSLSQLNIWDYVLSPQQIRSLASSCPSDLQRGNVFAWPDFLLGVTGRVKTSPQSEFCADCPLLQDAVPNLLSSSSAVSPGSQVKFSCNPGYYLVGEPVQRCLNRGTWSHAQPQCERVNCGAPPPLEHGQYHGEDFYAGSSVEYQCKPGFYLLGESKMQCTNSGMWASNPPACLDVDECALGSDCDDHASCQNTDGSHICTCIYPYTGDGRNCTEPVKCKDPGHPDFGHRDGNVFIVGSEVVFACEDGYELVGSSRLSCSETGTWEGTFPYCTALSCSPPTVPAHGIMKGTNFTYRSKVTFSCDNGYILDGPTEILCQSNLKWNSAPPSCQPVMCSEPPTVEFAEFTFNSKVYLSTLSYTCMEGYRLQGSGELRCEASGEWTSPVPVCARVDCGEPPPLKDAVIKGDNFTLGSHVQYICKQGFTLLGAETRECLPSGEWSHISAQCVPRQCGSPPHVDHALSDAGHQLFGDTAIYFCDDGYTIGNNTKLFCNAEGVWAPPEGFSMPHCTANFCQRPPDLPHAILDSINKPKYVSNTEVSYKCEEGFVLNTTATLKCLMGGEWSPSPLDIGCVPVRCSKPESIDRGYISGTNYSFGAVVAYSCDKGYYIRGEKRRTCKANGEWGGVLPTCQPIACSSPPRLANGYIQEPSRKASYVYSSKVTYACNAGYRLVGKPERMCMANKQWSSTDPPVCVLLICPTPPEVKHGHYRGSRFEVGSKVEYICDEGYDLTGDAVWTCLKYGKWDKSKTPHCSPVKCPEPPLEENHLILRSFDSDSGTVELSCEDGYVLHGARTLRCTQSQEWNTSFPVCKQVSCGPPPEVSFGDPSPASSYFGGVVSYSCMAGFTLRKEASISCQADGSWSTPYPECIPVECPHPEEIPNGIVDVQGLMYLSTALYSCKPGYDLVGNSTVLCGQNGLWIGGVPACHPIECPPPKDIPNGSAKYSRYQFSRSVTYSCQRGYRLEGPETLTCLESGKWDMEVPSCKQVYCSPPKPIENGFVEGRDHKFGVTIFYSCFPGFLLVGQNHLTCEEHGWSSSVPTCIPADCGLPPHIDFGDYFKIQDQYAELSRGDSKPTGQPSPADTSFLHGTMVQYRCHAGYDVNGGVALVCQEDGTWNGTAPVCVPAECETPPQPEHGSAIVTDTALGSLVEYSCEDGYELDGQTVRQCISGGQWSDEAPHCIPVNCGDPGGIADGEVVGSSFHFKGVIHYECHDGFILQGLETRTCQMDGKWDGKAPCCRPVSCGRPVVSSYVLVNGDSYTFGKRLLFSCNPGFILHGATTSVCLANGTWSESTPVCLPADCGQPPIIKNGRFRGTNYGYNGKVNYECDVGYILTGNPTLVCRGDGLWDDPPPRCDIVTCDPPEDISHGFLNGSSFNFEDVVEYVCFLGYEIVGSPILRCAAEGSWLGEVPECRPCMCNPPVLKFGAVLGRDHTCGASVWFRCDDGYKILGPTEAVCDKGGMWSPGVPICSRSRCSTPPPALPNAVVIGSPATLDTVTYGCRPGYFLRGHPNLSCGRLGRWGIPNLHCDPVSCGEPPPVPNGMIVGSAFTYGSKAQYRCTEGYEMVTKTDSVNCQSDGTWSKHSVRCRPVPCSLPTNLTNIVVVGARLTPVGGTVTISCKPGFYLEGPGLSECGLFGRWSPSFSSNSCVPVICEKPLPLLHGNIEGKSYNYGDMVMYTCLPGFELQGDNVQICQRDKTWSGAQPLCVAISCSSPPFVENAVLVATGESYESNVTYFCNSGLELIGSQSLTCQADGTWSLPAPTCKVPEGCARPDRILNGKLQEHSLASGKALEFFCDKGYTLQGESLVACMGNGSWSSPFPICTPKTCPPPPGWTGNTTVFLVGQTLSFRCPKGQQVKGQKGKKLATITCRSDRTWSPVSAVCERVSCGPPLHMANGVVRGAVFQFGDVAVYSCFAGYVMDGISRSVCLENGTWTPSPACRAICWLQCQNGGVCQRPNICSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCECPAGWTGTRCHSAVCSTPCLNGGRCIRPNRCHCSQGWGGHDCTRKRKAGYFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CX76</accession>
    <name>A0A3B4CX76_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017568927.1">
        <property type="nucleotide sequence ID" value="XM_017713438.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CX76"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015960"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024314.2">
        <property type="protein sequence ID" value="ENSPNAP00000015960.1"/>
        <property type="gene ID" value="ENSPNAG00000022057.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436755"/>
    <dbReference type="GeneTree" id="ENSGT00940000153431"/>
    <dbReference type="OMA" id="YNHIARY"/>
    <dbReference type="OrthoDB" id="6496718at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052224">
        <property type="entry name" value="THAP_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927">
        <property type="entry name" value="AGAP005574-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46927:SF3">
        <property type="entry name" value="THAP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="10">
        <location>
            <begin position="115"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="THAP-type" evidence="10">
        <location>
            <begin position="376"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="672"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="679"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="879" mass="100085" checksum="22FE4EF1264926C9" modified="2018-12-05" version="1">MSFSRFDEVEKYLRFGIYTQHDKASKGVLRRCCKKFRWAGDALWYIDGKRQRKVLRSEEEVRAVLVEQHNNRNHAGRKRCLKAVSDLYYWRSISKDITEWIDNCEGCSHPSNPIIRLQCIVPGCESSGLVDNEDGVTFHRFPFGEPSRLNCWISFAQRDRWSIHSRSAICSKHFTEDSFERSGDRVFLKCDAVPTLESSAYPQEVSNECWVDDSMESVEDEHPSVDEDLVHIEHPYSKGHTVQTSANGSSMNSPLLISTSEPYFRKYDALEKYLRTGVYSSMPYCRRDAVKRCAKRFCLQDGVLHYRHGSRLRRVLRSREEVINVLKEHHDKGGHFGLKRCLNSISELFYWGTITRDIEEWIGNCRFCMEMDEATQKYRCAVYSCNNYNGPLERMLGLTFHRFPFDEPERLNLWIRNLHRVNWQPRPRSVVCSTHFTEDCFDHSGASVCLKPHAVPTLLLCTTQDNEGEDSAYGLDEKSVSCETQDDLSAQHAFFSKYDAIGKYLNGKGYPTGLTSVEKNTFRRLCSRFAIHDGFLFYTSGRGIQEHGRRRVLRSKEEVQSTLTVYHDDMNHLAFEKCLRLISRHFFWGSLKADVALWIQRCPQCSGSEEPPGIEPCASPGSPEVTELLEPMEDQYSLGLAGQTNGRMTTENDLHLSISEVAVEQCVISSGPKRLPSCQPDSNTSDGTVTIRTDSVTSSSGSRQSPEDLAQERRNYSLSARTVVQQCTHALLQVKPQVAADADSEWVEIHEGMVIYVCFYKGATEKIIPKMVNSLLNAKFFPTSNGRHLSILEVPGSVLIVPQDTMTGVMKGSRVQHHDSVERWKGLHLYKRFVSLCEKEVAASTKCSEAGAVVRHGVYGNMQAIYLNSSGPLTHVIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CYY9</accession>
    <name>A0A3B4CYY9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017554606.1">
        <property type="nucleotide sequence ID" value="XM_017699117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CYY9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016533"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025081.2">
        <property type="protein sequence ID" value="ENSPNAP00000016533.1"/>
        <property type="gene ID" value="ENSPNAG00000022698.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428245"/>
    <dbReference type="GeneTree" id="ENSGT00940000156166"/>
    <dbReference type="OMA" id="HSRKHYR"/>
    <dbReference type="OrthoDB" id="6155966at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000245">
        <property type="entry name" value="zinc finger protein Gfi-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000208">
        <property type="entry name" value="zinc finger protein Gfi-1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000432">
        <property type="entry name" value="zinc finger protein Gfi-1b isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="120"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="252" mass="28535" checksum="78BDC8DF1B099210" modified="2018-12-05" version="1">MPRSFLVKKRRRGGSRSPLPTDCTPYLGIPSTAPIVSPITREEPEQSVESSSTQEANIHQDFCNSWTTDTKSFSEGSSLVPWHHLQATSTEDKQELGLLQPSNKDRDCSQISALNSSSTTHCPNCSKFFSSLSSLEAHVYKYHQGYSLPVVYCNSNKPVTPYRTKERTFDCQVCGKVFKRSSTLTTHLLIHSDTRPFPCQYCGKRFHQKSDMKKHTFIHTGEKPHVCRVCGKAFSQSSNLITHSRKHYRPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ47</accession>
    <name>A0A3B4CZ47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Double-stranded RNA-binding protein Staufen homolog 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">STAU2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017551701.1">
        <property type="nucleotide sequence ID" value="XM_017696212.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ47"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017362"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038497.2">
        <property type="protein sequence ID" value="ENSPNAP00000017362.1"/>
        <property type="gene ID" value="ENSPNAG00000023606.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426595"/>
    <dbReference type="CTD" id="27067"/>
    <dbReference type="GeneTree" id="ENSGT00940000154977"/>
    <dbReference type="OMA" id="PQNITEM"/>
    <dbReference type="OrthoDB" id="10037267at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032839">
        <property type="term" value="C:dendrite cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098964">
        <property type="term" value="P:anterograde dendritic transport of messenger ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007281">
        <property type="term" value="P:germ cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008354">
        <property type="term" value="P:germ cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035418">
        <property type="term" value="P:protein localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19880">
        <property type="entry name" value="DSRM_STAU2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19882">
        <property type="entry name" value="DSRM_STAU2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19884">
        <property type="entry name" value="DSRM_STAU2_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19886">
        <property type="entry name" value="DSRM_STAU2_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19888">
        <property type="entry name" value="DSRM_STAU2_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000073">
        <property type="entry name" value="Double-stranded RNA-binding protein Staufen homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000007">
        <property type="entry name" value="Double-stranded RNA-binding protein Staufen homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000024">
        <property type="entry name" value="double-stranded RNA-binding protein Staufen homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000013">
        <property type="entry name" value="double-stranded RNA-binding protein Staufen homolog 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051740">
        <property type="entry name" value="DRBM-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044476">
        <property type="entry name" value="STAU2_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044464">
        <property type="entry name" value="STAU2_DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044473">
        <property type="entry name" value="STAU2_DSRM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044474">
        <property type="entry name" value="STAU2_DSRM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032478">
        <property type="entry name" value="Staufen_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46054:SF1">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING PROTEIN STAUFEN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46054">
        <property type="entry name" value="MATERNAL EFFECT PROTEIN STAUFEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16482">
        <property type="entry name" value="Staufen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="78"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="165"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="277"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="377"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="8">
        <location>
            <begin position="561"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="126"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="650"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="656"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="674" mass="73050" checksum="4C92A60FE7DAA325" modified="2018-12-05" version="1">MAQVKVQTSAPQSVPGLVPSVPAVPSPGALGLQPTVNGTVPTTMPACSSPPAGFVDSTVPASPPGPPQDNMANPKEKTPMCLVNELARFNRIQPQYKLLNERGPAHAKIFTVQLCLGEQEWEAEGTSIKKAQHSTATKALAESSLPRPPPRSPKVDTNSNPGSITPTVELNGLAMKRGEPAIYRPLDPKPVPNYRANYNFRGIFNQRYHYPVPKVFYVQLTVGNKEFIGEGRTRQAARHNAAMKALQALRNEPIPERPPQCPEEKKGVEENSDASKSEISLVYEIALKRNLPVSFEVLKESGPPHMKSFLTRVTVGEFSAEGEGNSKKLSKKRAAFSILQELKKLPIIPVVEKPKLHYKKRPKTILKTGPEYGQGMNPISRLAQIQQAKKEKEPEYMLLSERGMPRRREFIMQVKVGAEVTTGSGPNKKVAKRAAAEAMLLQLGYKASTVQDSPDKSQMDNKGWNGQRAGFPEASANTQKGILHLSPDVYQEMEASRNKGSSGASVSFMSTKELGQGSAGPFYSSPGSSSTASMARELLSGTPTGEGLKGKPTTLTCSSVQPSQQLEYLAHIQGFQVQYSDGQTDKEFMTYLTLSPVQMTFHGIGSSIQASHDQAALSALKQLSEQGLDPVDGPIKVENGSCEIQAKHLAERTDSKQTNSGTTAQDCKDSKAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3B2</accession>
    <name>A0A3B4D3B2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="10">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GCLC</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:13286"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a catalytic heavy chain and a regulatory light chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="10"/>
    <dbReference type="RefSeq" id="XP_017549637.1">
        <property type="nucleotide sequence ID" value="XM_017694148.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3B2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017374"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026139.2">
        <property type="protein sequence ID" value="ENSPNAP00000017374.1"/>
        <property type="gene ID" value="ENSPNAG00000023665.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425473"/>
    <dbReference type="CTD" id="2729"/>
    <dbReference type="GeneTree" id="ENSGT00390000011908"/>
    <dbReference type="OMA" id="IAHMFIR"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.960:FF:000001">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000003">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="631" mass="72296" checksum="B9EC9449D0C41E5B" modified="2018-12-05" version="1">MGLLSQGSPLNWAETKKYADHVRKHGILQFLNIYNKVKDRQKDVLKWGDEVEYMLVELDDKHEKVHLVLNGKEVLETLQEKGEKINPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMGKRRREASSVLNENETLCTITSFPRLGCPGFTKPEFKPTPVEKGVSKSLFFPDEAINRHPRFSTLTRNIRHRRGEKVAINVPIFKDKNTPSPFVEVFPEDDGEAARAALPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATFCPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNKFRIHKSRYDSIDSYLSCCGEKYNDIELTKDEEIYKQLLDAGIDNLLAQHIAHLFIRDPLSLFEEKIHLDDENESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFIVLLTRVILSYKLDFLIPLSKVDDNMKVAQKRNAVQEGMFYFRKDIFKGCNLVFDSASTAAQNGVECDGDGEEYTLMSIDTIINGKEGVFQGLIPILNCYLDNIEVDVDTRCTILNYLKLIKRRASGELMTMAKWMREFVAKHPHYKQDSIITNKINYDLLRKCDRIAKGQEGCPELIGDPVNRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D3E8</accession>
    <name>A0A3B4D3E8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Nuclear receptor subfamily 2 group F member 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">V-erbA-related protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Binds DNA as dimer; homodimer and heterodimer with NR2F2 and probably NR2F1. Interacts with THRB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569800.1">
        <property type="nucleotide sequence ID" value="XM_017714311.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D3E8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017404"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038519.2">
        <property type="protein sequence ID" value="ENSPNAP00000017404.1"/>
        <property type="gene ID" value="ENSPNAG00000023674.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437297"/>
    <dbReference type="CTD" id="406522"/>
    <dbReference type="GeneTree" id="ENSGT00940000160748"/>
    <dbReference type="OMA" id="VETQFRC"/>
    <dbReference type="OrthoDB" id="5873264at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06958">
        <property type="entry name" value="NR_DBD_COUP_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06948">
        <property type="entry name" value="NR_LBD_COUP-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000016">
        <property type="entry name" value="Nuclear receptor subfamily 2 group F member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000020">
        <property type="entry name" value="Nuclear receptor subfamily 2 group F member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 16" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="18">
        <location>
            <begin position="50"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="19">
        <location>
            <begin position="164"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="406" mass="44113" checksum="8596E2CDBB13AA5F" modified="2018-12-05" version="1">MAMVGGGWGNPNGSTNGLGEKGYLQGEEDESSPQAGNSDAEGGEDDKICVVDCVVCGDKSSGKHYGVFTCEGCKSFFKRSVRRNLSYTCRSNRECQIDQHHRNQCQYCRLKKCFRVGMRKEAVQRGRIPPSHSGISPTSLVGGGGGGGGVGGPAMGGDFFNGQPAPELITQLLRAEPYPNSRYGAQCGQQLGGGNSAVMGIDNICELAARLLFSIIEWARNIPFFPDLPVSEQVALLRLSWSELFILNAAQSALPLHMAPLLAAAGFHASPMSAERVVSFMDQVRVFQDQVDKLTRLQVDSAEYSCLKAIALFSPDACGLTDPAHVESLQEKAQVALTEYERMQYPTQPQRFGRLLLRLPSLRAVPANLISQLFFMRLVGKTPIETLIRDMQLSGSSISWPYVPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D455</accession>
    <name>A0A3B4D455_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RNF121</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017556646.1">
        <property type="nucleotide sequence ID" value="XM_017701157.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D455"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019127"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028753.2">
        <property type="protein sequence ID" value="ENSPNAP00000019127.1"/>
        <property type="gene ID" value="ENSPNAG00000025675.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429434"/>
    <dbReference type="CTD" id="55298"/>
    <dbReference type="GeneTree" id="ENSGT00390000013075"/>
    <dbReference type="OrthoDB" id="446635at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16475">
        <property type="entry name" value="RING-H2_RNF121-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000074">
        <property type="entry name" value="Ring finger protein 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040176">
        <property type="entry name" value="RNF121/RNF175"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13407:SF1">
        <property type="entry name" value="E3 UBIQUITIN LIGASE RNF121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13407">
        <property type="entry name" value="RNF121 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="231"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="332" mass="38465" checksum="DEC46039144AA095" modified="2018-12-05" version="1">MAGVFEVEVDGVEHDHGHGVHDEAAQIDLSQLSPEEKWRLEHARMHAKHKGHEAMHAEMVLILIVTLVVAQLVLVQWKQRHPKSYNLVTLFQMWVVPLYFTTKLHWWRFLVTWFIFSVVTAFITFRATRKPLECTTPRLVYKWFLLLYKISYTTGIVGYAVVMFTLFGINLIFRIKPEDAMDFGVSLLFYGLYYGVLGRDFAEMCADFMASTVGYYSASGMPTKHLSDSICAVCGQPILVDVDEEGIMENTYRLSCNHVFHEFCIRGWCIVGKKQTCPYCKEKVDLKRMFSNPWERPHVMYGQLLDWLRYLVAWQPVIIGLVQGINYTLGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6J1</accession>
    <name>A0A3B4D6J1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017557562.2">
        <property type="nucleotide sequence ID" value="XM_017702073.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017557563.2">
        <property type="nucleotide sequence ID" value="XM_017702074.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6J1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018746"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028121.2">
        <property type="protein sequence ID" value="ENSPNAP00000018746.2"/>
        <property type="gene ID" value="ENSPNAG00000025187.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429970"/>
    <dbReference type="GeneTree" id="ENSGT00940000164700"/>
    <dbReference type="OMA" id="KTHRCHG"/>
    <dbReference type="OrthoDB" id="8667566at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF235">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="727"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="839"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="973"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1001"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1049"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1102"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1147"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="1191"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="227"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="275"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1017"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1231"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1017"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1033"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1245"/>
            <end position="1256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018746.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1256" mass="140939" checksum="10E2381FA9D0420A" modified="2024-11-27" version="2">MLGLQETKSAGTPPSKTYSCVACSATFNGLSSLLVHQASHASEISDQKAAMLPTCSHCSGLFASPELLQKHHCVVLPPPVTQDVYACDCGEEFHELEAIQEHKKIHESKTHVKIQETSNEQSETIEQSETIEQSETNEQSEMNEKSELTEPSELAQMDCHSSPVPSNENSPSLCTDESLHQNTQTASEECISTSDSHSVQNTISEVHHDEGSEPSDLTSPLVLTEAQDQSLENKVENVTSENTCVTDSEQTQEESQESSNKKSLLKMLASAYMHCRQPAQKQSEQSKRTLPPRKVSPRAPVQAPVMSASSSGYFINQLIQKVTKYGPDRMGFRQSSNMLSVKPVKKKKKKRIVSTTKTLYPVVALETCQKLVRDNIEGRHQCGLCRRVFQDVDSLIMHHALHKKERVKFCRRCRQYVISVISVPHNHICASSVGFSRHFTLLASNSPTMQHSRKLFHCTKCNRSYTRRHRLNKHNCQWLASLKHSAAGVQSDDKVSNFEEDSNTEKQIMSVQQDLSCQVNVGVNTGVLKLVKTEELDTDSLLEGKYQTGLSAKCPPLNSGLHIGSAKSFPPFRPNVFKFSSVQSRELVHSFAPTAAQDEMRPGSDTEAGESLGESQWTVPLDDAEIDVMDVDEEKHDDLMEAPKDNSDEVVVLEPTVKSPTSSILSCKDFQVHISPGGVKRFSCNRCQRTYSRRFTVKQHLKICGARKLMEQHSFGKANIIALKKKFPCPNCGTSFTRKDNMNMHRKRCQSMRSAGTLEAIKLSEKKNQASQENIFVLPPVSKNQVTQESSNNNSGSGNWGIMSLPSVLPRKVTCECGVAFTCPRLLFEHLQLHAMESYICSHCGENLQSWAEFEAHQKFHTQTSVQKSEQKVSQPQQPQLQQVPRFQLAVQNQNNQSRLNHRPLAEPCVCHRCKKVFRRRKSLLRHLRLSCRGDIAGQNKHSCSRCGMTFQSPLAHKVHVQGNTCTPSFKPIRCPVCVRWFSCVDGLKRHLVSHSQQKVLTCQICEHKCSSHEDLEEHKRRVHGAKEAAESPTIQSAHVSQSNPSNAFQCQICHRSYSKLQSLKDHLRKVHRPQGLKPGEPKVGGLQSLVGTVQQGQSKQFQCHICSRTYPDIQSLKNHKRRVHRILGSGIQPSKGSLQQSHSNPFQCHICSRSYPDLRSLRNHRRRVHRILGGLEMFKGIVHQTQGTPYRCQICQRSYPDVRSLKNHRRRVHHILGDVPESAKVVATDVEQSEVKSEPLDVTLSPSTAPETAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D6U8</accession>
    <name>A0A3B4D6U8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuole membrane protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">VMP1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017579439.1">
        <property type="nucleotide sequence ID" value="XM_017723950.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037402630.1">
        <property type="nucleotide sequence ID" value="XM_037546733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D6U8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018654"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027961.2">
        <property type="protein sequence ID" value="ENSPNAP00000018654.1"/>
        <property type="gene ID" value="ENSPNAG00000025107.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443352"/>
    <dbReference type="CTD" id="81671"/>
    <dbReference type="GeneTree" id="ENSGT00390000007230"/>
    <dbReference type="OMA" id="EEPYDKR"/>
    <dbReference type="OrthoDB" id="2016540at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="45704" checksum="9A1D346985E478A6" modified="2018-12-05" version="1">MAENGADCDLPQKRVGPKENRNGNFTDASLRERKQQDREERLSLVLWRKPLLTLQYSFLETLVTLKEWTGRLWQRRGAVFLVLVLFSVFSMAYSIEGAHQKYVQYLEKKILWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSVNFPEPPYPAQIICPEEEVVQDSVSLWTIMSKVRLEACMWGAGTAIGELPPYFMARAARLSGAGPDDEDYEEFEEMLEQAQSAQDFATRAKLAVQNLVQKVGFLGILACASIPNPLFDLAGITCGHFLIPFWTFFGATLIGKAVIKMHIQKLFVIITFSRHIVEQMVSLIGVIPGVGTSLQKPFREYLEAQRTKLHNPVADGTPAGENWLSWVFEKVVLVMVCYFILSIINSMAQSYAKRLQQRKYSEEKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D731</accession>
    <name>A0A3B4D731_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547609.1">
        <property type="nucleotide sequence ID" value="XM_017692120.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D731"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018739"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028078.2">
        <property type="protein sequence ID" value="ENSPNAP00000018739.1"/>
        <property type="gene ID" value="ENSPNAG00000025205.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424226"/>
    <dbReference type="CTD" id="559392"/>
    <dbReference type="GeneTree" id="ENSGT00950000182864"/>
    <dbReference type="OrthoDB" id="6274823at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000631">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="455" mass="52569" checksum="C4EAE35CCE259E5F" modified="2018-12-05" version="1">MLSPVLCSELSALEPEYTEADVISAVEFSQSGEFLATGDKGGRVVIFQREPQGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQLNSAHFLLTTNDKTIKLWKVSERDKRPEGYNLKDEEGRMKDISKITSLQVPVLRPMDLLVEASLRRVFANAHAYHINSISINSDCETYLSADDLRINLWNLDITDRSFNIVDLKPENMEELSEVITVAEFHPHHCHLMAFSSSTGTTRLCDMRSRALCDQHAKLFEEAVDPANRSFFSEIISSVSDVKFSHSGRYLLTRDYLSAKVWDLNMENKPIETYQVHDYLRSKLCTLYESDCIFDKFECAWNGTDSVIMTGAYNNFFRMFDRASRRDVTLEASREVCKRRAVLRPRRVCVGRKKRESDVSVDSLDFRKKILHTAWHPTDNIIAIAASNNLYIFQDRHSHHGHHAGHRLEQDRPPQLQDVPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9M1</accession>
    <name>A0A3B4D9M1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plac8 onzin related protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cornifelin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578537.1">
        <property type="nucleotide sequence ID" value="XM_017723048.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9M1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019669"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029659.2">
        <property type="protein sequence ID" value="ENSPNAP00000019669.1"/>
        <property type="gene ID" value="ENSPNAG00000026305.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442818"/>
    <dbReference type="GeneTree" id="ENSGT00940000163927"/>
    <dbReference type="OMA" id="TLMQHEK"/>
    <dbReference type="OrthoDB" id="1045822at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13957" checksum="49A6E16EF97B4576" modified="2018-12-05" version="1">MAVQQQVVSVTTHTTHSSGTWTTGLCDCCSDMGTCCCGLWCFPCLQCQAVSQFGWCFCLPLLDCCLTVSCCLRSKMRERYGIHGSCCDDCCTLCFCYPCAWCQMSREIKIRAHATSSTVVTQQIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9Q0</accession>
    <name>A0A3B4D9Q0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017580385.1">
        <property type="nucleotide sequence ID" value="XM_017724896.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9Q0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020225"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030653.2">
        <property type="protein sequence ID" value="ENSPNAP00000020225.1"/>
        <property type="gene ID" value="ENSPNAG00000027011.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443970"/>
    <dbReference type="CTD" id="64812"/>
    <dbReference type="GeneTree" id="ENSGT00940000165992"/>
    <dbReference type="OMA" id="MYSLMEH"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF283">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="59"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="59"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="290" mass="32338" checksum="C810B73395B254CB" modified="2018-12-05" version="1">MMYSMMEHELKSTAVQQSSHHGGQVLQAGGGCGNAPPHQHHQQQQAAQKNSCPDPMDKVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLTDAEKRPFIDEAKRLRAVHMKEYPDYKYKPRRKTKPLLKKDAPPGKYPLTAAQGQALSPRMDGYGWAPGGGYTAMQSDALGYTQQLHRYDLSGLQYPTHTYMNGANSYSPMSYSSSPQQPSPVMSMVKPEPLPHSPTGASNHHRGAIQGDLRDMISMYIPGGDVGDPTTQRGYPSVQQHYLGSTVPLTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDP4</accession>
    <name>A0A3B4DDP4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metabolism of cobalamin associated D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MMADHC</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDP4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021605"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033050.2">
        <property type="protein sequence ID" value="ENSPNAP00000021605.1"/>
        <property type="gene ID" value="ENSPNAG00000005431.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425955"/>
    <dbReference type="CTD" id="402886"/>
    <dbReference type="GeneTree" id="ENSGT00390000015050"/>
    <dbReference type="OrthoDB" id="10263782at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009235">
        <property type="term" value="P:cobalamin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019362">
        <property type="entry name" value="MMADHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13192:SF3">
        <property type="entry name" value="COBALAMIN TRAFFICKING PROTEIN CBLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13192">
        <property type="entry name" value="MY011 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10229">
        <property type="entry name" value="MMADHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="34414" checksum="1029E7D3F44C3A6A" modified="2018-12-05" version="1">MFCPSVDCKQEKPNRMTSVLCSRARLVTYLPGLHVLVRRITAARSFSGASGSDEPHLNITPPDMGLRTVWPDETMGPFGPQDKRFQLPGNAGFACHLEGIAPQKTNVVHRTVPDVLTAQSSSERHEFVLAQFVGEFHGKDEASSQNVGSVEKYFDHSKVECAIQSCPELLKKDFELMFPEAPSSGMMVVTVTQKTENDMTAWSEEVDQEREELLAKFIAGAKEICLALRTEGFWADFIDPSSGLAFFGSYTNNTLFETDERYRHLGFQIEDLGCCKVIRHSLWGTHVFVGTVFTNAPPNSLIMKKLQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEN8</accession>
    <name>A0A3B4DEN8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Insulin receptor substrate 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">IRS1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017574790.2">
        <property type="nucleotide sequence ID" value="XM_017719301.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEN8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021531"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032899.2">
        <property type="protein sequence ID" value="ENSPNAP00000021531.2"/>
        <property type="gene ID" value="ENSPNAG00000004388.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440437"/>
    <dbReference type="GeneTree" id="ENSGT00940000161579"/>
    <dbReference type="OMA" id="MTMQMGC"/>
    <dbReference type="OrthoDB" id="946068at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005158">
        <property type="term" value="F:insulin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01257">
        <property type="entry name" value="PH_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01204">
        <property type="entry name" value="PTB_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000029">
        <property type="entry name" value="Insulin receptor substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000129">
        <property type="entry name" value="Insulin receptor substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039011">
        <property type="entry name" value="IRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10614">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10614:SF11">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00628">
        <property type="entry name" value="INSULINRSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="13"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="6">
        <location>
            <begin position="142"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="243"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="450"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="698"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="957"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021531.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1098" mass="118396" checksum="9DF44532A8A35283" modified="2024-11-27" version="2">MASPSAEQGCFSDVRKVGYLRKPKSMHKRFFVLRAASAAGPSRLEYYENEKKWRHKSGAPKRSVPLESCFNINKRADSKNKYLVALYTKDEYFAIAADSELEQDAWYQVLVDLHNRGKVHENAGSNGIGEDSYGETTPGPAFKEVWQVILKPKGLGQTKNLTGVYRLCLTNKTLGFVKLNSDAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMKAMSEEFRPRSKSQSSSNCSNPISVPVRTRHHHNNPPPSQVGLGRRSRAESVTATSPAGMGKHSHSFRVRASSDGEGTMSRPASVDGSPGSPSAVRPQSQRHRGGSRLHPPLNHSRSIPMPSSRCSPSAISPVSLSSSSTSGHGSTSDCLFPRRSSASISGSPSDGGFISSDEYGSSPCDFRSSFRSVTPDSLGHTPPAKEEELNNYICMAKPGSLTSNGSQSQSQSRGTPSRLDEPELEKCFRKRTHSSGTSPPTLCHQKTPSQSPAASLDEYTVMTPAYSNSRSASSSSSTYRNSFMPARLYPEEGLEMPPTEGSSGLSHKDDGYMPMSPGVAPSVVTGKSGDYMPMSPKSVSAPQQIINPRQHSRVDSNGYMMMSPSGSCSPDVSTNYGKIWTNGVNPKLSVESTEGKVSSCGDYINMSPASCSTTGTPPDCYFNPVDEPSRPAYAYFSLPRSFKHTNKKQDQSPLRMSQGASRLVCADSSSSSASSDSLGGQCNSHPPAVRPKRSEVDSRLARPTRLSLDANKASTLPRMRESPFPPEPKSPGEYVNIEFDDKAFSASLASLFSPVFTGNGADVLSEPPSSDYMNMQLGSRRLLSDSRIPMASSSSDYAIITPSETSASSHPLPCESICERDYISMQLGASCTAYTNSQMMLMNASLPDSEVPLCSDSPNDDIATLRGGPGRPVLIGPLTGLSAFTRVNSTPARDQGAKVIRADPQGRRRHSSETFSSTPTRGAVGTAAGFQSEDVKRHSSASFENVWLKPAEVAAAASPAASASTGRRETLTAASLAHQNQNGLNYIDLDLAHNREQPVPDWSLFQARQAELEATGGAEELRAYASISFQKADETRGNLSHREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DK81</accession>
    <name>A0A3B4DK81_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ras-specific guanine nucleotide-releasing factor RalGPS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RALGPS1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017540086.1">
        <property type="nucleotide sequence ID" value="XM_017684597.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017540087.1">
        <property type="nucleotide sequence ID" value="XM_017684598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DK81"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023868"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011587.2">
        <property type="protein sequence ID" value="ENSPNAP00000023868.1"/>
        <property type="gene ID" value="ENSPNAG00000000941.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412534"/>
    <dbReference type="CTD" id="9649"/>
    <dbReference type="GeneTree" id="ENSGT00940000154079"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13310">
        <property type="entry name" value="PH_RalGPS1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="581" mass="65746" checksum="596BB0149B91CE2E" modified="2018-12-05" version="1">MYRRNGLPASVSITSATTQDSSSSESLDGRSLDSAKSYDAVVFDVLKVTPEEFAGQITLMDAPVFKAIQPEELASCGWNKKEKHSLAPNVVAFTRRFNQVSFWAVREILTAQTLKIRAEILGHFIKIAKKLLELNNLHSLVSVVSALQSAPIFRLSKTWALISRKDKATFEKLDFLTSKEENYTRMREYIRSLKMAPCIPYLGIYLFDMTYIDSAYPATESIIETEQRANQMNNLLRIISDLQMSCNYDHLLTLPHVQKYLMSVRYIEELQKFVEDDNYTLSLRIEPGNSSPRLVSSKEDLGGPSEESVSVRYNRRPTCPDTSVVAHLPTPPPARHRKSHSLGNNMMCQFGVVESKSATFSAKEKARHLLDDSFLESHSPVRNHTHDSVFTNGISLGSRESSFSDELSSTVERGRMYATLGPNWRVPVRNSPRSRSYVYSASGAVSSYECSSLSSITIEGPLRRKTVMKEGKKPTLSSWKRYWIVLSGSTLIYFGSKALRANERRHYKSRPCKKISLTGWIVVLPDDPEHPNIFQLNDPDKGNVYKFQTGSRFSAIIWHKHLAEACRCIRPQIPANLMSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DSB7</accession>
    <name>A0A3B4DSB7_PYGNA</name>
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        <recommendedName>
            <fullName evidence="13">[histone H3]-trimethyl-L-lysine(27) demethylase</fullName>
            <ecNumber evidence="13">1.14.11.68</ecNumber>
        </recommendedName>
    </protein>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(27)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
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            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
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        </reaction>
    </comment>
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        <cofactor evidence="1">
            <name>Fe(2+)</name>
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        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the UTX family.</text>
    </comment>
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        <property type="gene ID" value="ENSPNAG00000011248.2"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
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            <begin position="1862"/>
            <end position="2025"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1396"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1458"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1517"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1529"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1542"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1585"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1761"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1824"/>
            <end position="1839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2166" mass="238970" checksum="FDBDB42DD5A9CB5E" modified="2018-12-05" version="1">MMLQLSQDGIICVLEFTKQLSGRASRERERDSDRGLTGPGRSRSQTGRGQQTAGRSSRGALWGRRRTSCTCMRTSVTAEFSGAAVDKFSSVSVCCPLSAAVEGFTHTGSVVSNCRINIQINTCLETIWCQFISGMLIVSLPSATLRLLIFSFYYYVLFTGWMHHTAEQFGARSTRDPFPLDGLNRGTWAPVSSRAWPPPARCSPGISQSQFLPNIPPSHMAGLNHPSKFFNSGPLARGGDKLELSQALLPGLHRVPPATPRPWEPIGQHYDQLPSDDHFRLHNGYNTGPLAHLTARANQLLKYGPLSAHHGSRQMPPLCNDIWAQSQQQQQAPPTRDPYSHASQLKRSAPPLGEQSVIQHTPHPQNRPSEDCPSPSKRKRSSESDQHGAYQFPGPIGSSLSSLQQPQSHCPPQKQGFWNPLQKGNASWSTAERKVESTEFQDLRKPGIDDFSYKQSPSSKLSPLSPPPISTPGGYGQGRGPPTQRHKPSLSPQSMGSSSHSAHLNDRTYPYPNNKSSAQTQGEQQGIPSHRAHDSGVSATRSQAPPPRPSLTQSNRDCPAPTPAHHATVPYSHPNFQPHPGLVHTTSSSSSSRTQATAPQCNSQKPWKNQTKHGHHIPDSGPYHPLVEPQVTPSSLQQKQNGTRENQDASTPQHLSPPQALTRGPVITPNMPSLEISSPSCSLGLYSSGETVPTVSSLPSTNSAVASTVKSWKATDSSELISETNPAPSTHQGYRGSQFQGTHHQQEAKTQRKPPPLPHEDQGRPHYTQIVSSVPSTLSSGFQRSGSSVITSRASNNLPQVANSPLHHPQSAAPNTVNTAPQPHSDPLYPKMPHHAAPAVSQVPPAQAYQKPCQQPAISSPESIEDTLKKLAELQDHMQAEERRREQEEEEQKQKRERKEISMKVHEESAIESLERLLSNSATDPPPPRLSPAIAPSSVSPPSQTSPPWLSRGGVPNRPPVPGTASLERSRPPPLTPQTDYAREKQRQREQWNSRASLSPPSQNTSGIPTHTYPSKPTPADSSSNQTILSTSCLSKDSPIQKSPHEVRAMGAIPNPPNLREPPKLYQAFPRDTLPSCQPTGTSSGNLHKSMPSGGLGSLGSSASSCSGDSDSAQFEEEQSELFPDGLANIMKMLDESIKKEEELYSGQSGTQTCSKQTFSVTVAPMKSYLGAPDLMPALKQPSSEDYLTDTHASPPVLSRQGSLASPCSRTSSLEEEEEMLKIIPKPANTQHQDSGVSITGTNYRHSDLAKLYGLPEAVKSECEEDEEEAEQEDDTPSCSPPHMRPHLHQTGVNSMFKNLASVLESQKYAYRGGPFGRPPPSALVGVKYSSSLSLGPDICRQQSTSPTSGSTSHPGFSNPTQAPTNTTSEQTHPQSPSVCAAERKGGPLSSQSDLSGHESEEGMRKGPTDKEDTCDVKVSKEVEGRPKLMTISESSLAELGRSCEVMLNRHKLPSKALLDHSKPETQDMYKPEKEKDRNRERERERDPERKHKHSSKKHEERKERKKKHRERREEMSLSSSSSSSSSSSRRHKEGKPHKEKRGNVDLQIKEIREKDREQDGEKKKRKEEGSEREEWVSWNKEKSGNSSDRSSSSSALGSTDFQKLKALTDGPPKELKIRLIKVESGDRETFIASEVEEKRIPLQEISIKNTASEIIRSCKAARVKGKFKESYLLPSFSVKPVFPMEHPIPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKSLVEANADQAVEVRTQVQQPADENWDPSGTGQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKGSDDDDDDDEKKPVSNSDSTNSNSTNSGSSPEQKSVGKIIKFGTNIDLSDPKRWKPQLQELQKLPAFMRVSSSGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHDNYWKAISDFCEKHGVDYLTGSWWPVLEDLYRSNIPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLNAYQYQLALERFEWNEVNKVKSIVPMIHVSWNVARTVKVTDPDTYKMIKHCLLQSLKHIQILRGQLVAAGKKISYQSRVKDEPAYYCNECDVEVFDLLFVTSENGSKKMYVVHCEDCARQRSPNLSNVVVLEQYRIEDLMNTYDSFSLASAPSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DSS8</accession>
    <name>A0A3B4DSS8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fibroblast growth factor receptor-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Has a negative effect on cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017559198.1">
        <property type="nucleotide sequence ID" value="XM_017703709.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DSS8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004922.2">
        <property type="protein sequence ID" value="ENSPNAP00000026513.2"/>
        <property type="gene ID" value="ENSPNAG00000001952.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430892"/>
    <dbReference type="CTD" id="497135"/>
    <dbReference type="GeneTree" id="ENSGT00940000156736"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005007">
        <property type="term" value="F:fibroblast growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000016">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000246">
        <property type="entry name" value="Fibroblast growth factor receptor like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000593">
        <property type="entry name" value="Fibroblast growth factor receptor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052615">
        <property type="entry name" value="FGFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890:SF10">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0732">Signal</keyword>
    <keyword evidence="2 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043938221" description="Fibroblast growth factor receptor-like 1" evidence="15">
        <location>
            <begin position="24"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="27"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="150"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="247"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="123"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026513.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="486" mass="54017" checksum="66355087BC1BA4F4" modified="2024-11-27" version="2">MIPDREKVFILAGILMMALSCEARGPPWVTRGVEERQTAQLGRTVRLPCPVEGDPPPLWHWMKDGRTVSPGWSRFRVLRGTHSLKIKEVEAEDAGVYVCRAINGFGSVTLNFTLSVIDDATVPENKPPSLEEPTIEPTQHDPTGQPLAKPRFSKPEKMRRRFREQPVGSSVRLKCLASGTPDPVITWWKDQVQLPGPARGNQYQWTLTLKNLQPQDSGRYTCRVSNAAGYINYTYKVDVIERTNSKPILTGTHPVNTTVEFGGTASFQCKVHSDVKPVIQWLKRVEPGTENRYNSTLEVGGQHFMVLPTGDVWSRPDGSYLNKLAIVKARDEDAGMYICLGANTMGYSFRSAYLTVLPDPQVEMPVPPAHLSSGLPWPLIIGIPAAALLMMGTIVLWLCHSRLRQGSLPPRPVPHRDHHAPDKDPSSPSNGSSDFPVQRFIGIGAPALSGPPKIYSKVYTDTHTHTHTHSHAHVDGKVHQHFHYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DT57</accession>
    <name>A0A3B4DT57_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MyoD family inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MDFI</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDFI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550220.1">
        <property type="nucleotide sequence ID" value="XM_017694731.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DT57"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005486.2">
        <property type="protein sequence ID" value="ENSPNAP00000026286.1"/>
        <property type="gene ID" value="ENSPNAG00000011579.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425779"/>
    <dbReference type="CTD" id="4188"/>
    <dbReference type="GeneTree" id="ENSGT00940000160187"/>
    <dbReference type="OMA" id="HCPHHTH"/>
    <dbReference type="OrthoDB" id="8958154at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026134">
        <property type="entry name" value="MDFI/MDFIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304:SF1">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15316">
        <property type="entry name" value="MDFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="27488" checksum="9B250131B19B6DFE" modified="2018-12-05" version="1">MWEEEHSPSACSPEPPDGAHPAPETSSVEPPTAPSSGGDGSSENQAQEPPTPPTAEKTSEADNGNNNNSLSQLDFSSTPSKSITCQPQARSTPLLPSLSSSSEAAALLKNGSKPVTHQAHSNGVKNGAPLLHAASSSSSSSSPSHKNPKKLQSNPSLNSQSSKRSKGSSKSNSSQIPMEAQDDCCVHCILACLFCEFLTLCNIVLDCATCGSCASDDSCFCCCCASEECGDCDLPCDMDCGIIDACCESADCLEICMECCGLCFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTM4</accession>
    <name>A0A3B4DTM4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017551150.1">
        <property type="nucleotide sequence ID" value="XM_017695661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTM4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026818"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004147.2">
        <property type="protein sequence ID" value="ENSPNAP00000026818.1"/>
        <property type="gene ID" value="ENSPNAG00000002124.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426295"/>
    <dbReference type="CTD" id="447809"/>
    <dbReference type="GeneTree" id="ENSGT00940000157993"/>
    <dbReference type="OMA" id="QIRIECT"/>
    <dbReference type="OrthoDB" id="27389at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04402">
        <property type="entry name" value="RhoGAP_ARHGAP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047886">
        <property type="entry name" value="ARHGAP20-like_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047887">
        <property type="entry name" value="ARHGAP20_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE ACTIVATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179:SF26">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22286">
        <property type="entry name" value="RHG20_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="279"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="471"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="652"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="861" mass="95549" checksum="96293D72BF3D8B20" modified="2018-12-05" version="1">MRRGSYDDAAAPIRPHLRSLAQRRRSAPNLMFGKALGMSWSPIREEAPFPMAVEQSPFVLGLATEDSELLLDDTVQLTEGLKTRERHLFLLSDVLVIAKLKSCASYRLKHRVNLEDLWVFGFKSEDDEVDDEDGEIDLKTTVLLAWSLSLCLVSFSLPEVKERWLDTLHRKVREVRKRTGCTSPPPSVLMKVLSSTTTNKTLSGGGMDSVIELPLHGNKISALPKELCKQEAHTEPSTADHERSTGSKRSLFSFKLKRSSTHSGLPTRSENRKSLLFGQPLARHATPPKPITELLVLLWRKGPATEGIFRKACNSRNLSAVREQLDSGAEVDMEALPVALLVGLLKTFLKELPGSLLVVEHSDEWVEALEKEEPEEKQNELRRMIGKLPEANSVLLQYLLCVLHHIAQHSERNKMDIRNLAVCIAPNLLQDNSQLLDVVKVEKVTKLTQFLIENCVELFGEKILTLLGDPEEEEMGDNSDSMSSHQHDSAYDSTDPDGDGESGEALGPQEEEEGALGNCQIRIECTPVPSCSSDTIFHPKPFNRRSSEPTIFPPTSIKSLRGLARSQDDCSSAREEYEDQPLKKQISDDSFLHPHSCHRLLTRKLAGSLNMDAMINGTRSAPISKACSCSSSCSLESAASNVSETSVFMVASSPVPSPGTARKSQASSKARADTNKADLDLRRHSSPFKRAKSLGSFSTTRGSAKKADCQKDVGFSCETLQEDSQNEAEGPEELVRRQRPLSAIEVFQHVDSRIPNCPPSYTQAMLSGSLPAPPQYRKMTVHEARKSRPISMNDCILSSCPAAQFTDLDSFELSDSNVVQQGSFRQRAMSESVARSRFEKVNRRCSQPVFEEFSYAKESYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUA7</accession>
    <name>A0A3B4DUA7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 25">Receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3 25">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24 35">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3 25"/>
    <dbReference type="RefSeq" id="XP_017557988.1">
        <property type="nucleotide sequence ID" value="XM_017702499.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUA7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027048"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003590.2">
        <property type="protein sequence ID" value="ENSPNAP00000027048.1"/>
        <property type="gene ID" value="ENSPNAG00000012443.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430177"/>
    <dbReference type="CTD" id="378478"/>
    <dbReference type="GeneTree" id="ENSGT00940000155450"/>
    <dbReference type="OMA" id="KNTMATQ"/>
    <dbReference type="OrthoDB" id="6219513at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048408">
        <property type="term" value="F:epidermal growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005006">
        <property type="term" value="F:epidermal growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010647">
        <property type="term" value="P:positive regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050679">
        <property type="term" value="P:positive regulation of epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023056">
        <property type="term" value="P:positive regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05108">
        <property type="entry name" value="PTKc_EGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000001">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000002">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000044">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000006">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Receptor tyrosine-protein kinase erbB-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044912">
        <property type="entry name" value="Egfr_JX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006211">
        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000494">
        <property type="entry name" value="Rcpt_L-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049328">
        <property type="entry name" value="TM_ErbB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016245">
        <property type="entry name" value="Tyr_kinase_EGF/ERB/XmrK_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF91">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00757">
        <property type="entry name" value="Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01030">
        <property type="entry name" value="Recep_L_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21314">
        <property type="entry name" value="TM_ErbB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000619">
        <property type="entry name" value="TyrPK_EGF-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0010">Activator</keyword>
    <keyword evidence="12 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 25 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 31" id="KW-0732">Signal</keyword>
    <keyword evidence="20" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17 25" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017342025" description="Receptor protein-tyrosine kinase" evidence="31">
        <location>
            <begin position="22"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="32">
        <location>
            <begin position="711"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1157"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="26">
        <location>
            <position position="836"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="717"/>
            <end position="725"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27 28">
        <location>
            <position position="744"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRNR" id="PIRNR000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000619-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1214" mass="134474" checksum="71B626F59D26E518" modified="2018-12-05" version="1">MANSAEAGFLLTFLLLSSGWCVTPEKKVCQGGNNRLTLLSSVDDHYQNMVKMYSNCTVILENLEITYTTDKHDLSFLKTIEEVGGYVLIALNQAPEIPLTNLRIIRGHTLYSDNSFALAVLSNYNQSTRMGMKKLPLTRLTEILKGGVRFANNPSLCHMETIQWADILNMKSSPLDLELSNKFCENCDSSCYNGSCWALGPENCQTFTKLTCAEQCSGRCKGPKPSDCCNEHCASGCTGPRPTDCLACRDFQDEDTCKDACPPLMRYNPNTHQLAPNPDGKYNFGATCVKSCPHNYVVTDHGACVRACSPGTHEVDEDGVRKCKKCDGLCPKVCSGLGVGHLTNVLSVNASNIDSFENCTTINGDVLILKTTLTGDIFTKTPKMDPARLNVFKTVKEITGTLLVQYWSPTMPSLSPFENLEVIRGRTKYQGTISLGVSRLPITHLGLRALREISDGDVSITYNENLCYNSPEHWTRLFKSDKQSARRMENANEAACAAQNRTCDEMCTSEGCWGPGPTMCFSCEHRSRKRSCVASCNLLEGEPRECEVNKTCIECDPECKPLNGTLTCKGPGPDGCEECANFRDGPHCVARCPQGVPGERDTLVWKYPDSMRVCQLCHQNCTQGCAGPDLKDCRDFKPYSGMSVIAAGVVGGLLAFVILSLGVAVLIRRRHIKRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWVPEGEDVKIPVAIKVLREATSPKANKEILDEAYVMASVEHPHVCRLLGICLTSTVQLVTQLMPYGCLLDYVKENKDNIGSQHLLNWCVQIAKGMSYLEERHLVHRDLAARNVLVKTPQHVKITDFGLAKLLSADEKEYHADGGKVPIKWMALESILHRTYTHQSDVWSYGVTVWELMTFGTKPYDGIPASEIAGVLEKGERLPQPPICTIDVYMIMVKCWMIDADSRPRFRELIAEFSKMARDPTRYLVIQGDERMHLPSPTDSKFYRSLMSGVDLDEAVDADEYLVPNHGFFSSPSTSRTHLLHSVSLNSSIGSCLNRNGNLFPVRENSLVLRYIPDPTDQFLDGDFQPAPEYMNQQNGAVLSDMSNPNYQHPAPPRTAHPAGVLDETEEEYLNCFKNPAASSGISPPEYLNTSQAHLNNSQARLLSPSSSSHFRPNGHSFIAQSSIDNPDYQQDFCPLGVKPKANGHLPAAENAEYMGVEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DV99</accession>
    <name>A0A3B4DV99_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">H2AX</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DV99"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027001"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003684.2">
        <property type="protein sequence ID" value="ENSPNAP00000027001.2"/>
        <property type="gene ID" value="ENSPNAG00000035495.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230360"/>
    <dbReference type="OMA" id="RRNICHA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000004">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="14">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027001.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="137" mass="14492" checksum="A106FE2B98ECA80D" modified="2024-11-27" version="2">MSGRGKTGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTGQALKGNSTTKDGLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVA6</accession>
    <name>A0A3B4DVA6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin C1-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DVA6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027380"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002716.2">
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        <property type="gene ID" value="ENSPNAG00000012876.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000275826"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:immune response"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
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        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF191">
        <property type="entry name" value="CLASS I HISTOCOMPATIBILITY ANTIGEN, F10 ALPHA CHAIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043310347" description="Immunoglobulin C1-set domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin C1-set" evidence="3">
        <location>
            <begin position="129"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027380.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="289" mass="32851" checksum="E2CDDCB71FCC55B3" modified="2024-11-27" version="2">MRANLCLLCFAGSHSLCSVATFIIGETPFPEFIAPHYDKEQGVAKMVFGALHKRMLKCANFLKQHFNYTNGVHTEQRLDCCELLSNNTAGQMRMIDAFNGLRITYLGQVISPPVILQEFAYFPTHDSGGASVTCLITGFYPRHINLTLLRNSQPVSEHLVSGGELLPNGDETYQMRKSLRATHSFTNVCRSSLQKLQRSLILIPFLFHCYTELLRGNISVISHNKFLYDTECLKITNKKTLTDVSHIFGWIKMIFYVLINANTSLHFINIWNTKHTLCHYKGRNMGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVE1</accession>
    <name>A0A3B4DVE1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BAR homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017551958.2">
        <property type="nucleotide sequence ID" value="XM_017696469.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DVE1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026939"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003843.2">
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        <property type="gene ID" value="ENSPNAG00000002188.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426748"/>
    <dbReference type="GeneTree" id="ENSGT00940000165215"/>
    <dbReference type="OMA" id="FTIDWIL"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF5">
        <property type="entry name" value="VENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="120"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="122"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038196"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026939.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="264" mass="30086" checksum="0207CC759CE30685" modified="2024-11-27" version="2">MVKNFSVDWLAQSYHDSTQDPEHLDATAAPRRHVPCSVQPRPPTSYDKGYLQPKPKCTEQKIPTTRLMEKEPVLFDPLTPRSCSSPSSSENSGYSSGYESEAASSECPSVEDVNEEKDLGSQRRIRTKFTLEQIDKLEKIFSKHKYLDAGERVKTALKLGLSETQVRTWFQNRRMKLKREVQEMRGDYLVPALAPVFFPGVPGVQHHCYSGQRQALAPLMMPPLALQKTPFPHQHMIPPLSVQQASSHHQYMIPQHSHAMQLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DYN1</accession>
    <name>A0A3B4DYN1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Globin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">HBE1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the globin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DYN1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028535"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014785.2">
        <property type="protein sequence ID" value="ENSPNAP00000028535.2"/>
        <property type="gene ID" value="ENSPNAG00000014815.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157809"/>
    <dbReference type="OMA" id="AVIANMW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031838">
        <property type="term" value="C:haptoglobin-hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005833">
        <property type="term" value="C:hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031720">
        <property type="term" value="F:haptoglobin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043177">
        <property type="term" value="F:organic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08925">
        <property type="entry name" value="Hb-beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000001">
        <property type="entry name" value="Hemoglobin subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002337">
        <property type="entry name" value="Hemoglobin_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050056">
        <property type="entry name" value="Hemoglobin_oxygen_transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442">
        <property type="entry name" value="HEMOGLOBIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442:SF7">
        <property type="entry name" value="HEMOGLOBIN SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00814">
        <property type="entry name" value="BETAHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="8">
        <location>
            <begin position="3"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028535.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="16317" checksum="4769597BD4A8E851" modified="2024-11-27" version="2">MVEWTEFERATIQDIFSKMDYESVGHNALARCLVVYPWTQRYFGKFGNLYNAAAIMGNPLVAAHGTVVLHGLDKAVKNMDNIKAAYAELSVLHSEKLHVDPDNFRLLADCLTIVVAGRLGSGFTPDVQAAFQKFLAVVISALTKQYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZC1</accession>
    <name>A0A3B4DZC1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IFI44 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017538107.1">
        <property type="nucleotide sequence ID" value="XM_017682618.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZC1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028354"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000201.2">
        <property type="protein sequence ID" value="ENSPNAP00000028354.1"/>
        <property type="gene ID" value="ENSPNAG00000013999.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411182"/>
    <dbReference type="CTD" id="565601"/>
    <dbReference type="GeneTree" id="ENSGT00940000163581"/>
    <dbReference type="OMA" id="SLMEKPW"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF19">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="2">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="439" mass="48981" checksum="9C01D5B610753FE5" modified="2018-12-05" version="1">MSVVTSRLSRQQEQKLCSMLGCNRLSLLFKASVHGYTGNAFHQKCDRQGPTVTVAYNNSGYIFGAYTSRDYAQTNQNFTDDKAFLFSFNEREMKKDPLRVVSGAGQHSLYDGTGGPYFHSLVFLYNEAATVYSNPGNYPFDPTEMHGNDLQLVECEVYRVEGSGSFMEKPWRNVLWNFERRKALMDMITGWTHGVSSVKQARVLLVGPVGAGKSSFFNSINSVFKGYVSSQANTGCAGTSLTTQFRTYSVKAGKEGKLLPITVCDCMGLEEGLNAGLDIEDFTSILRGHMQDKYRFNPSMPLQPESNSFLREPSMTDRIHTVVYVIDACKVKLLSDRMIEKLVAIRRKANQMGVPQLVLLTKVDEACSSVAADLKTVYQSLYIHKMVQEISADLGVSMSAVVPVKNYSQELELDPQTDILLLNAVIQMLRATESFFDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZM0</accession>
    <name>A0A3B4DZM0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">GABA(C) receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577988.1">
        <property type="nucleotide sequence ID" value="XM_017722499.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZM0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000028516"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034996.2">
        <property type="protein sequence ID" value="ENSPNAP00000028516.1"/>
        <property type="gene ID" value="ENSPNAG00000014272.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442458"/>
    <dbReference type="CTD" id="570876"/>
    <dbReference type="GeneTree" id="ENSGT00940000159906"/>
    <dbReference type="OrthoDB" id="442503at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19059">
        <property type="entry name" value="LGIC_TM_GABAAR_rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000007">
        <property type="entry name" value="Gamma-aminobutyric acid type A receptor rho2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000005">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit rho-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022262456" description="GABA(C) receptor" evidence="18">
        <location>
            <begin position="23"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="20">
        <location>
            <begin position="73"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="21">
        <location>
            <begin position="279"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="403"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="404"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="473" mass="54939" checksum="2785316031BDE5BA" modified="2018-12-05" version="1">MKPTALALRLLYLAWLWPTTLLSESQFPSRRKHKDVYLGENTRHKHGGRIDFKMKKSDSTKSLLIKSEQLLRIDDHDFAMRPGFGGSAIPVGIDVQVESIDSISEVNMDFTMTLYLRHYWQDDRLAFPSSSNKSRTFDSRLVKKIWVPDVFFVHSKRSFIHDTTMENIMLRVYPDGNILYSVRITVTALCSMDFSSFPLDTQNCSLELESYAYNENDLMLYWKNGNDSLRTDEIVLSQFFIEEFQPSYGLAFYSSTGWYNRLYINFILRRHIFFFMLQTYFPTMLMVMLSWVSFWIDRRAVPARVSLGITTVLTMSTIITGVSASMPQVSYVKAVDIYLWASFLFVFLSVIEYAAVNYFTTVEEMKKLKRGKLPVSYDATQAMAYDGCFHDNELDLSPFPELPTTPSTEQAAPPRNSSAEPPPTEGTRLWRKRSVRHNINFIMSNSYMIDSYSRIIFPVAYLLFNIIYWSLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0P3</accession>
    <name>A0A3B4E0P3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">OCIA domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the OCIAD1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E0P3"/>
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    <dbReference type="Ensembl" id="ENSPNAT00000016243.2">
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        <property type="gene ID" value="ENSPNAG00000015288.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063690"/>
    <dbReference type="OMA" id="YYEFTQE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040187">
        <property type="entry name" value="OCAD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009764">
        <property type="entry name" value="OCIA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13336:SF4">
        <property type="entry name" value="OCIA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13336">
        <property type="entry name" value="OVARIAN CARCINOMA IMMUNOREACTIVE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07051">
        <property type="entry name" value="OCIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OCIA" evidence="6">
        <location>
            <begin position="44"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029275.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="16245" checksum="698F5A05D9CFFCF9" modified="2024-11-27" version="2">MDRSSLSGFTGSDHPGETTPLPAEQTDHTSAQLPSASGKGPLGMDYIPTEEERRVFKECNQESFWYRSVPISVSSMALTQFLVSRGQLSSMYSSPRFGSVPKVLFAGACGYFAGKLSYMKTCQEKFKRLENSRLGEALRQRAGHRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E1Q2</accession>
    <name>A0A3B4E1Q2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E1Q2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029164"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035374.2">
        <property type="protein sequence ID" value="ENSPNAP00000029164.1"/>
        <property type="gene ID" value="ENSPNAG00000015047.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164685"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000069">
        <property type="entry name" value="calcium-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000037">
        <property type="entry name" value="calcium-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043582">
        <property type="entry name" value="CaBP1/2/4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45917">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45917:SF13">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="95"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="206"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="237" mass="27141" checksum="5541A7F33513AA22" modified="2018-12-05" version="1">MPMSVISMEAGSMDTIEVPQDKIVKKGSIKKKIEKLRRWSTSSGGSFKRPPKEKTLSLRQPTETEEDSGEGERVRRKLRPMVASVFGQDRDLRPEEIEELKEAFMEFDKDRDGFISCKDLGECMRTMGYMPTEMELIELSQQICGGRVDFEDFVELMGPKMLAETADMIGVKELRDAFREFDSNGDGQISLAELREAMKKLMGEQLNTREIDEILRDVDLNGDGLVDFEEFVRMMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E5J9</accession>
    <name>A0A3B4E5J9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PDZRN4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E5J9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031045"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019651.2">
        <property type="protein sequence ID" value="ENSPNAP00000031045.2"/>
        <property type="gene ID" value="ENSPNAG00000018179.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183062"/>
    <dbReference type="OMA" id="TMEQDYY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd06716">
        <property type="entry name" value="PDZ2-PDZRN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000028">
        <property type="entry name" value="PDZ domain containing ring finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051971">
        <property type="entry name" value="E3_ubiquitin-PDZ_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545">
        <property type="entry name" value="PDZ DOMAIN CONTAINING RING FINGER PROTEIN 3, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545:SF6">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING RING FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="143"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="402"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="500"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="449"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="512"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031045.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="749" mass="84991" checksum="F47875E37A78115B" modified="2024-11-27" version="2">MGCNLCSLQKREEHYKLLYEISQVNGKDLSRASHEEAVEAFRSAKDPIVVQVLRRNPLPRGQGSSQDVHLVDVCTQTDITFEHIMALAKLRPSSPPVPDICPFLLSDSCHSLHTIGQEYYEGSEYLSTMPADAERTDELEYEEVELCRLNSQEKLGLTLCYRTDDEDDIAIYVSEIGPNSIAARDGRIREGDRILQINGQDVQDREEAVAVLSSEDCRNIVLLVARPEMPLDDAAWLDDEHSEFLEELKMEMLEEQRREEEEMERAKSSESYQERTCGAGAQRHCSDTSDHNVLAQIQKRLSQCLRQRREAVGQRSESPGLARCSGEGLEGEESEGDRYQQLLELKCQIRNSGEYDLFYSRSSTIECSLTEQSGGVERELRLLNEELRSIELECQSIMQAHQLRKKDQRQSVPAKEQLQWSPRRGCGQQQGSKLANINEHPEKSDKDSSSAYNTAESSRSTPLAMDRSPEHSLQRMVSLTNQKNLKSSLSSLIAGCGSPPCPAVRRAPSGSPDHSNPSESEQTPPAEDESSPKKGKALDSRIPYLSHRHQQADVPAHARHYQSYMQLVQQRSAVEYGQSQLSLLAAGGPQAPRPSEPKMEWKVKVRSDGTRYITKRPVRDRLLRERALKIKEERSGGMTTDDDAMSEMKMGRYWSKEERKQQLLRAKEQRRRRELMQRCRLDSLKESPQSSGEGRKEVSIIELSHRKMMKKRNKKILDNWMTIQELMTHGAKAPEGAKVHNAFLSVTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E844</accession>
    <name>A0A3B4E844_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E844"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020452.2">
        <property type="protein sequence ID" value="ENSPNAP00000031461.2"/>
        <property type="gene ID" value="ENSPNAG00000019107.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156118"/>
    <dbReference type="OMA" id="LEMKWHA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902495">
        <property type="term" value="C:transmembrane transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052076">
        <property type="entry name" value="TRP_cation_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47143:SF1">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47143">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL PROTEIN PAINLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="692"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="734"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="796"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="836"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="901"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="314"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="452"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="518"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="728"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031461.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1072" mass="120404" checksum="17942D0DF98EEC7E" modified="2024-11-27" version="2">VHSVRHSYLDGSPCRCKVRDDSSSASFFSALEKHPKQLAVRDDSGASPLHYAVSRGHLRAIKLIVQVAGLLELNAVDEEGNTPLHWAVQKDQRGSCSTLLGLGADPNILNKCLLSPLHLAVSLRHNGLVAELLFHSLTDPNLEGDLGNTPLILAASLDNHEALYEQLSGCFWTYDVSPHYIFSTVASLLHVGEEAGMSIEAHINCLDRSISSPLHLAVRGGNLEVIELCIAHGAKIDQQQCDKSTALHFACTQGAIEAVKVMLNAYDRVCDIINITDGACQTPLHKAAIFDHCELAEYIISQVIGADIDFIDCKGHSPLLLATSCGAWRTVNLLLSHGADLKVKDKAGCNFLHLAILQPRGLKNLPPEVLQSVRELLNDEDSEGCTPLHYACRLGIPDSVKNMLGLEVSLDQKSKQKKSALHFAAEYGRINTCHRLLETMTDTRLLNEGDKKGMTPLHLASRCGHVKVVELLLRKGALFHSDYKGWSCLHHAAAEGFTQTMETLLISNIKLLDKTDGDGNTALHLAARAGHVAATRLLLCRGAQIILNKGEASFMHEAVQNGRKEVTIAVIDSDRCEEVITTFKLNSAKRCIVMDMIEFLPESFKHLLDTCIKESDEDVNSCNYYVEYNFQWLQQPEQYAKKTENSEKEDNYKPLAALNAMVEFNRVELLTHPVCKKYLEMKWNAYGVKAHLLNMTVYALGVFPLTYLIGLISLAKSHLCRIGHVLTSFSQQCYFITTCMFLVLAMNMYAVGKEVVQMFQQRLKYLQDVSNLMDWAAAICSLLFVLPLLLDVKSSWHWQAGALAALISWFNLLLYLQRFERFGIYVVMFREICRTLLSIIVLFVYLILAFALAFYALMIEQRHFGRMFLSLMQTFVMMVGEINYQDNFLKPYMVAKLPFPVLTYGIFVFFVLLVPILLMNLLIGLAVGDIAEVQTNACLKQIGMQIELHTNLEERLPHWFMKRVDQASVKEFPNKSCKSKVTIRSVTPRLSPTPRQSTPMERELAKQKYRLKEISEFMEKQHNLLKLVVQKMEISSEAEDNDGPPVFQEFKQKLLARSRWGPLLRAVTARKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E8M3</accession>
    <name>A0A3B4E8M3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017556874.1">
        <property type="nucleotide sequence ID" value="XM_017701385.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017556875.1">
        <property type="nucleotide sequence ID" value="XM_017701386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E8M3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062470.1">
        <property type="protein sequence ID" value="ENSPNAP00000038197.1"/>
        <property type="gene ID" value="ENSPNAG00000035641.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429556"/>
    <dbReference type="CTD" id="557421"/>
    <dbReference type="GeneTree" id="ENSGT00390000009230"/>
    <dbReference type="OMA" id="QSIDEWY"/>
    <dbReference type="OrthoDB" id="6247020at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045955">
        <property type="term" value="P:negative regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037658">
        <property type="entry name" value="CBARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28597">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT-ASSOCIATED REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28597:SF2">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT-ASSOCIATED REGULATORY PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="730"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="670"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="787"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="880" mass="96684" checksum="A686482F5C02469C" modified="2018-12-05" version="1">MSNESAVWKNLTESPTELPLEPGGQDGYVLLLVLLSIFLGGTLVLLSVLLIACRRCCEGDRRYTRFILCFRASDDPEKTTASYLEESQPVHEITIRVDESDCVSAGSSHDAETERFLSTGTTGRRVSFNEAALFDHNKKTQEKGRRYTLTEGDFHHLKNARLTHLHLPPPALKIVTIHECESSENSIAMTTRPVAKSSLSIFQPPICPLPQTALVSLSISPSSALPGDALNSTVDTSFCESPPAPGPQPPSPSLMEVRRLSPVSRLEGASLSATGLSSSGGAPQGPVLQFFTKLRRHASLEGASPYFKIKRWKFDSSQRASSLDTRGSPKRRPFQRQRAASESMDEEEHGAHHIDLIQYIARTKDMAYCPSRPAPRLLSPPSTPPPSLGRLEVEVMVEPSCSRAMGPGVIGLSAEPQDEAPGMGYRRESTDTQTLYRDIWTLRASLEQYISSDQSSNNDKDSVHSDADSVCSVRLRMERGGLPRYPSEDTGDGMEGETELPGDAGLEKRGKRDSVDSERGSDGESGTRKLLQMDSGYASIEAPSRGPDEVRLFRSSSESKDGSAVERRHFFTSSGHRGTVCESFETDIFDEELEEEPMGASGGSDGLTELDEGVMVWSPYGQMFTQSDTTQPRAPVLSRRDYSIDEKTDALFHEFLRHDPQFDQQESPVRSKHRSRVHLRKQWQRSKQYSDPGQRFHSSFDRYRTPLRRGETVNYPLESSYHSTLPRIVSAPDEESSEGAASLPETPKADQSSAAASENVAALRDDHTGVSSSTDTSGDLKGKVEIASSTSLPLSESTVGQPEVPAEESGQMRPLLDPGDEHLPPQLTETGYGPQTITTELSDKLAMNLEERLYSGLRRTREQPECALTATHASPDHSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EH94</accession>
    <name>A0A3B4EH94_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 222b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EH94"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035230"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027935.2">
        <property type="protein sequence ID" value="ENSPNAP00000035230.2"/>
        <property type="gene ID" value="ENSPNAG00000025085.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007371"/>
    <dbReference type="OMA" id="GKMKQFH"/>
    <dbReference type="OrthoDB" id="267284at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008496">
        <property type="entry name" value="TMEM222/RTE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20921">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20921:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05608">
        <property type="entry name" value="RTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035230.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19788" checksum="F9DE0E3BBFAB43E9" modified="2024-11-27" version="2">MKNYQIAEEKIDPNSSRYPYCIVWTPIPVLSWLFPFIGHMGICTSSGVIRDFAGPYFVSEDNMAFGRPTKYWMLDVSKVYASGSNAWDTAVHDASEEYKHRMHNLCCDNCHSHVAMALNLMRYDNSTSWNMVNLCLLALIHGKHVSCAGFLKTWLPFLILTAVVVSLALYVNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EMA2</accession>
    <name>A0A3B4EMA2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NACHT LRR and PYD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EMA2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030856.2">
        <property type="protein sequence ID" value="ENSPNAP00000036399.1"/>
        <property type="gene ID" value="ENSPNAG00000027168.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286904"/>
    <dbReference type="OMA" id="ITEVGCT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="70" mass="7642" checksum="040D2C6C1BA97793" modified="2025-10-08" version="2">MENPHCKLETLGLKFCMITAEGCAAFASALKSNPSHLRDLYLSQNDLGDSGVKMLSVGLESSHCKLETLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ENA7</accession>
    <name>A0A3B4ENA7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor HES-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Hairy and enhancer of split 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017560375.1">
        <property type="nucleotide sequence ID" value="XM_017704886.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ENA7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036759"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031783.2">
        <property type="protein sequence ID" value="ENSPNAP00000036759.1"/>
        <property type="gene ID" value="ENSPNAG00000027873.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431616"/>
    <dbReference type="GeneTree" id="ENSGT00940000163346"/>
    <dbReference type="OMA" id="NNIQMEY"/>
    <dbReference type="OrthoDB" id="6085656at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097150">
        <property type="term" value="P:neuronal stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11461">
        <property type="entry name" value="bHLH-O_HES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000033">
        <property type="entry name" value="Transcription factor HES-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="15">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="16">
        <location>
            <begin position="90"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="123"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="163" mass="18791" checksum="2FCA9722ED0D0048" modified="2018-12-05" version="1">MTPAVASAMTISKEHLALNNKLRKPVVEKMRRDRINSSIEQLKTLLTPEFLNQQPDSKLEKADILEMTVSFLRRQQQKQPAFSTSSTAVSQGFSRCIHEIVNFLSKDEVKTQSQRKLLNHFQNLQPSTDKSNRKLVLPQTRCPDQQTMSTKENPVKSSLWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IH47</accession>
    <name>A0AAR2IH47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">RPS3A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569019.1">
        <property type="nucleotide sequence ID" value="XM_017713530.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IH47"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071890.1">
        <property type="protein sequence ID" value="ENSPNAP00000038368.1"/>
        <property type="gene ID" value="ENSPNAG00000020251.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436826"/>
    <dbReference type="CTD" id="6189"/>
    <dbReference type="GeneTree" id="ENSGT00390000018433"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03122">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_eS1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027500">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="234"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="243"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="265" mass="30055" checksum="3AC7E6EECA89DDD9" modified="2024-11-27" version="1">MAVGKNKRLTKGGKKGAKKKIVDPFSKKDWYDVKAPAMFNIRNLGKTLVTRTQGTRIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRTNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVYVRKVKMLKKPKFELGKLMELHGEGGASSAKPAEGDTGAKVERADGYEPPIQESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IX13</accession>
    <name>A0AAR2IX13_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translin-associated factor X-interacting protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IX13"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083482.1">
        <property type="protein sequence ID" value="ENSPNAP00000042136.1"/>
        <property type="gene ID" value="ENSPNAG00000013726.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183166"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012331">
        <property type="entry name" value="Clathrin_H-chain_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032755">
        <property type="entry name" value="TSNAXIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF11">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN LINKER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15739">
        <property type="entry name" value="TSNAXIP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translin-associated factor X-interacting protein 1 N-terminal" evidence="3">
        <location>
            <begin position="18"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="465" mass="53105" checksum="BE8574FC428DEC2D" modified="2024-11-27" version="1">MHFIYNNMLNLRSQLHSIIDVVIDYATAYEPILTAIKKEYDEFIAAVKTNDRKAKLARGKLKALVTQPTSLMYCQKRAAQLQERKNRRDSQQPEALDDQAQIPVGQIPGTLNSIRSVYFGLDYLQKCRHKKKSQYVPAQVKRELVIKMKSTLNQRDELGLENNKLQLRYGINKDVFEEDDPCKVRESELLMDYIERFPNHIKHGNEIDENHPYKNVILCVFYVIKKMTNVKLPPALLFFQALTISVQPGKRLPGEVLSLEGVRCALQHGYIQLVTFWVTQHRLTYSEELGDVICSHGAEDPRVADTCLALAHIVYTACGVLRKAALSMCRRGLISGAFEFIYKSRDFTVGETRHITGCPSLDLMQDFTQLYEGKPALLSVGFICHSLLKSDLEDLAFQLLEKIYKTILNDVMCSAESWSEIATRCEQRGWHQLAQKIISILLSQPGAVCLSQEQDSVELMEHVFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IY66</accession>
    <name>A0AAR2IY66_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ELKS/RAB6-interacting/CAST family member 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Presynapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IY66"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046378.1">
        <property type="protein sequence ID" value="ENSPNAP00000042541.1"/>
        <property type="gene ID" value="ENSPNAG00000003494.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00650000093320"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098882">
        <property type="term" value="F:structural constituent of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019323">
        <property type="entry name" value="ELKS/CAST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18861">
        <property type="entry name" value="ELKS/RAB6-INTERACTING/CAST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18861:SF1">
        <property type="entry name" value="ELKS_RAB6-INTERACTING_CAST FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10174">
        <property type="entry name" value="Cast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="552"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="750"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="945"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="336"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="787"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="552"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="750"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1022" mass="115982" checksum="9490A2E8DFE21914" modified="2024-11-27" version="1">MYGSSRSVSKMDGNHSGSRANQGNTGRSPRLPRSPRMGHRRTNSMGGSGGGPGGSGGKTLSMENIQSLNAAYATSGPMYMSDNEVALATSSDIPKSSAATGRFGGSIPYGVRGTVMGSTPDMAMVPAVSTDSMGFGSELHTASTVPHSLRQARDNTIMELQAQLKEVLRENELLRKEVEVKESKLSSSMSSIKTFWSPELKKERALRKDEASKVAVWKEQYRAVQEETQHLQTTVQALQAELRIQRDLNQLLQPPGEPLACEPGEEHERQARELFLMRRTLEEMELRLETQRQTLAARDDSVKKLLEMLHSKGPSTKASEEDHELTCRLADAEMHAHHLENMLEQRDKEIVALREELHRHLEGTPESTKTKALQTVIEMKDSQINSLERGLRELEDQVMMLRSSSMLSCEERQEEVKQMEVYRNHTKFMKNKMDQVKQDLSRKDTQLLGLQTKLETLTNQFSDSKQHIEVLKESLTAKEQRAAILQTEVDALRLRLEEKETLLNKKSKQIQDMSEEKSTLNGEIHDLKDMLDVKERKINVLQKKIENLQEQLRDKEKQMGSLRERVKSLQTDTSNTDTVLTTLEDSLAEKERIIERLKEQRDREERERSEELDSSKKELKELKEKVSLLQGDLSERETSLLDLKEHASSLASSGLKKDSKLKTLEIALEQRREECSKLENQLKRAQNAAVEAQANSELSERIASLEADVTRHREESGKAQAEVDRLLDILRQMENEKNDKDRKINELERQMKEQGKKPSGKHREPASKGRNTSLCSLNLVIMTCQSQESLRQKSERIEELEEALRESVQMTAEREMVLAQEEAARTHHEKQMEELLGAMEKVKQELESMKAKMSSTQQSLAEKEAHLTTLRAERRRHLEEVLEMKQEALLAAISEKDANIALLELSSSKKKKTQEEVAQLRREKDRLVQQLKQQTQNRMKLMAGNYDDGHLKTPPDQTNHKPPKSPDQVTLCCPFPFPPHATPSKAHLAEASVGFQGYVIVAQITAPPPPYPPEDITLELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZ00</accession>
    <name>A0AAR2IZ00_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZ00"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059115.1">
        <property type="protein sequence ID" value="ENSPNAP00000043302.1"/>
        <property type="gene ID" value="ENSPNAG00000031014.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234513"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF20">
        <property type="entry name" value="GAP JUNCTION BETA-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="11">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="12">
        <location>
            <begin position="161"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="291" mass="33341" checksum="017F3F5A5076CD0C" modified="2024-11-27" version="1">MLLTPRAELVNYLESKMNWASFYAVISGVNRHSTGIGRIWLSVIFIFRILVLVVAAESVWGDEKSGFTCNTQQPGCNSVCYDQFFPISHIRLWALQLILVSTPALLVAMHVAHRRHIDKKILKISGRGTAKDLEQIKNQKFKITGALWWTYMISIIFRIIFEVAFLYIFYLIYPGFKMVRLVKCDSYPCPNTVDCFVSRPTEKTIFTVFMLAVSGVCVLLNIAEVVYLIGKECVRYFQRPEGEPKGPWLAQKLSSYKQNEINQLISEHPFKPRLNVGRKSPIDKGERCSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZ44</accession>
    <name>A0AAR2IZ44_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZ44"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060890.1">
        <property type="protein sequence ID" value="ENSPNAP00000043319.1"/>
        <property type="gene ID" value="ENSPNAG00000031443.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000245198"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="1">
        <location>
            <begin position="128"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="228" mass="25264" checksum="D77208C35A03E2EC" modified="2024-11-27" version="1">MQTAEKYTIQRALSEQGQLLGQHQLVLSGVTRSLESLSNQQSEQQAQLVQLVNSIKEMSSHFRVTNLTDQTAVLTPTSPAAATPQTTFPVSKPDKFSGDPDQCRGFLLQCSVFFDNSPVGTDQAKISFVVSRLAGKALDWATASWSSFSNWTYAHFLKEFRLVFDNPYEGKVSGERLAQLRQGSHSVVEYALEFRTLAANGGLRPCIDYRALNQISKAYPYPLPLVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0T1</accession>
    <name>A0AAR2J0T1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CMTM3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037390247.1">
        <property type="nucleotide sequence ID" value="XM_037534350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0T1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079281.1">
        <property type="protein sequence ID" value="ENSPNAP00000045570.1"/>
        <property type="gene ID" value="ENSPNAG00000037005.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108415519"/>
    <dbReference type="GeneTree" id="ENSGT00940000160432"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF3">
        <property type="entry name" value="CKLF-LIKE MARVEL TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="27"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="171" mass="18724" checksum="D777C45D559377F1" modified="2024-11-27" version="1">MGDMEDPDRRGDHPNPSVVSLLFNKDFVSSRKGQLLIGEVASSFISFVCFCASSAAAFITAPLVFFLSALFLLFAYSTKFNQRFKGFHWPLMDFLRCVSASIVFFIISIMAVSKYPDGSSKAAGVFGFIATIVFALDFYFIFNELAAFLKGGGSHDEPQNTQEAGSDSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JA65</accession>
    <name>A0AAR2JA65_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PHD finger protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PHF14</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JA65"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057306.1">
        <property type="protein sequence ID" value="ENSPNAP00000048830.1"/>
        <property type="gene ID" value="ENSPNAG00000012397.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156923"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15674">
        <property type="entry name" value="ePHD_PHF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15561">
        <property type="entry name" value="PHD1_PHF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15562">
        <property type="entry name" value="PHD2_PHF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15563">
        <property type="entry name" value="PHD3_PHF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000086">
        <property type="entry name" value="PHD finger protein 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF150">
        <property type="entry name" value="PHD FINGER PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="290"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="353"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="697"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="840"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="26"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="780"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="891"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="200"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="797"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="808"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="897"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="920" mass="102370" checksum="45045514D703F1E8" modified="2024-11-27" version="1">MGDAEGSVDRTSKRRQVKPLAASLLDALDYDSSDDSDFEVGDAEGSGSEGTGNGSDEEGSKDSSAGTDSDSDAAGSADEEGVDEEEAKDLNEDAQEEEKAKEQPSSEETSSKETGGTSRSRKKSEKTTDTEPNGSGTTEESPAEPKKWNLRRNRPMLDFTTMEQLNEMDDYDSEDDNDWRPSAGKKKNKAAGKDKGGGSEGEEDEGSDEDDNDDDDDDDDDDDENDDDNENSSSDGEEVVKKSKKKAAKNTGAFDEEETNDSHSTSHGKGNEDSLLERAQPWSAQRMEHILICCVCLGDNSEDADEIIQCDNCGVTVHEGCYGVDGESDSIMSSASENSTEPWFCDACKNGVSPSCELCPNQDGIFKETDAGRWVHVVCALYVPGVAFGDIDKLRPVTLTEMNYTKYGAKECSLCEDTRFARTGVCISCDAGMCRSFFHVTCAQREGLLSEAAAEEDIADPFFAYCKQHADRFDRKWKRKNYLALQSYCKVSLHEREKQLTPEAQARITTRLQQYRAKAELSRNTRPQAWVPREKLPRPLTSSASAIRKLMRKAELMGISTDIFPVDTSDTSASIDGRRKHKQPALTADFVNYYLERNMRMIQIQDNISEQKNLKDKLESEQEKLHVEYNKLCESLEDLQNVNGLLRTEGQAMWSMMGAILGQKLNGPAILKAPKERKPSKKEGGSPGKSCSLPAVLYSCGICKKNQDQHLLLLCDTCKLHYHLGCLDPPLTRMPKKTKNSYWQCSECDQASSDEADIAMETLPDGTKRSRRQIKGPIKFIPQDMSPEPKKLQVRGTRTRGQKRKRVSICEEEKIEEPAPRERRQRQSALQKKPKADDTRTECTTCKGPGDNENLVRCDECRLCYHFGCLDPPLKKSPKQTGYGWICQECDTSSSKEEEEAREVEEESVKAETAEPECPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JC86</accession>
    <name>A0AAR2JC86_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein RD3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017545338.1">
        <property type="nucleotide sequence ID" value="XM_017689849.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JC86"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066558.1">
        <property type="protein sequence ID" value="ENSPNAP00000047844.1"/>
        <property type="gene ID" value="ENSPNAG00000034110.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108417108"/>
    <dbReference type="GeneTree" id="ENSGT00390000002089"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028092">
        <property type="entry name" value="RD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28489:SF4">
        <property type="entry name" value="PROTEIN RD3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28489">
        <property type="entry name" value="RENTINAL DEGENERATION 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14473">
        <property type="entry name" value="RD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25102" checksum="EEE4D4C15D5E0C0F" modified="2024-11-27" version="1">MFPWSAVFSLEPKVPGQRTPEELVTNTLMLELGAMVKRTERIRLERATEVRRRRGSSSSVDYSWLAGPQPHVPYELTPGDVLDLQELCAQIPPSQCGPVIVRFRKLVTGYEPEVHEVPQLFRSVLRACLEEARGDAELQARNGRCDKQRSKSLSFVTFRSKLRTLSRGRGSFGGSRGNLQEEPAWSDEEEEEEVAGHAGAVARARRGRSQSMPEIIPMEQAAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JEL2</accession>
    <name>A0AAR2JEL2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NFXL1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JEL2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058639.1">
        <property type="protein sequence ID" value="ENSPNAP00000048777.1"/>
        <property type="gene ID" value="ENSPNAG00000035702.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286971"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003288">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000761">
        <property type="entry name" value="Zinc finger protein 449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="559"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="587"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="615"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="638" mass="73343" checksum="922FA19E8B43ED98" modified="2024-11-27" version="1">MKTNSAKRGRPPRKKTEIIGTKCAKYTFVKTEPLPTEGVLINSEADKPPSQYPYNGTKDYSSSEINQMKQEPGHFQLQAAQIQELMQSSSSLQPLPSDFKTRFPKTEPHCVPIHLDDVELKAEPDIRDVVMNGERCLHLKEEQEDGTKAKLEATERFAERTLRQDEEPASNSILSCPYCSVSFTDFKYLEKHLKWTHRSNYLAWLKHHKAPPQCTKISSSMLSCSYCPNRFFTQRQLKAHMQRNHLPSPAPARKRYTCPQCDRSFDYIGNLQNHCRKCHDLDTLCTGGELSCSACGKSFAGMWGLGPHCCNELEEDEEHKPDLKSDRPLCTDRGFLCRHCGKNCPTLQSLTIHMRTHTGEKPHACRECGRGFSELGSLRRHMAIHTGIKPYECPECGKHFARMNHLSTHLRTHTGERPYQCSDCGMKFSHKATLHMHQRVHSREKPFSCPDCSKVFATLGNMKAHQLMHNQDRIYECEQCGKMFARPDVLKKHLHTHSGERPYLCTVCGKSFSRIQHLTNHQRTHTGERPYNCEECGTTFSQSGDLTKHMRSHTGEKPYACPDCDRRYNNSGDLSKHRRSHTGQRPYRCQQCNKAFLMPHHLKTHILTHTGERPHSCPQCCRTFIRAHHLTQHIIKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JFG1</accession>
    <name>A0AAR2JFG1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome-recycling factor, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ribosome-releasing factor, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MRRF</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563655.1">
        <property type="nucleotide sequence ID" value="XM_017708166.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JFG1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061695.1">
        <property type="protein sequence ID" value="ENSPNAP00000049062.1"/>
        <property type="gene ID" value="ENSPNAG00000033645.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433537"/>
    <dbReference type="CTD" id="92399"/>
    <dbReference type="GeneTree" id="ENSGT00390000005084"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.20:FF:000001">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000007">
        <property type="entry name" value="ribosome-recycling factor, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982:SF3">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME RECYCLING FACTOR PSEUDO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="RIBOSOME RECYCLING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="Ribosome recycling factor, RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosome recycling factor" evidence="9">
        <location>
            <begin position="91"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000049062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="254" mass="27916" checksum="354916D658E85B14" modified="2024-11-27" version="1">MACSRLGLLRPVLFYIRSPPPTCMRLPPLLQNVYPHVVGTPPCFVRHYATKKSKAKAKGQAAKVSINAALVEDIISLEEVKEDMATVLATLKDDFSRNLGIRTSAGALDHITVTTKDGKFPLNQLGQISMKSPQLIVVNMTSYPEATAAATRALQESSMKLNPEVEGTIIRVPVPKVTREHRENLAKLAKQFSNKAKESVRRVRSSALAKVKKSKEGVSEDTIRLIEKQVQQIADSYAADIDKQLATKTKELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JG31</accession>
    <name>A0AAR2JG31_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037397501.1">
        <property type="nucleotide sequence ID" value="XM_037541604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JG31"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044293.1">
        <property type="protein sequence ID" value="ENSPNAP00000050905.1"/>
        <property type="gene ID" value="ENSPNAG00000036535.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413848"/>
    <dbReference type="GeneTree" id="ENSGT00940000168127"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050853">
        <property type="term" value="P:B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050868">
        <property type="term" value="P:negative regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026072">
        <property type="entry name" value="Lime1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032748">
        <property type="entry name" value="PAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16322:SF1">
        <property type="entry name" value="LCK-INTERACTING TRANSMEMBRANE ADAPTER 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16322">
        <property type="entry name" value="PHOSPHOPROTEIN ASSOCIATED WITH GLYCOSPHINGOLIPID-ENRICHED MICRODOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15332">
        <property type="entry name" value="LIME1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="465" mass="49809" checksum="A339447A7D02BFA4" modified="2024-11-27" version="1">MDLLDLPYTKVLILGAIAAASAFVVTILIVLVCVGCQRKSKSKHAQSNGGSHKVDMGVLRQSKLSSMSKSDTRLHEMHRVPCNANGAPKNRPASMDLLLLHSRRSTSDLHPPGSSRQLPRIPTSPEVEEREHTYSEVGRRSSPTPRCLDDGLYESVGVKGPPPDPSTPSLAPPPTSTNTSAQGCTHSPPTNGNGRGGGGKAGPNEALTAEYATIRKVRKLDRGRRVNGSEEGESVSSTESSTGNLHRKTLEPFHLPNFPKEAVFMGNGEQYIWKPPEESDIVISGGSSLENGQSHPSAVEISEMYSTVCKTLKKKHQQQQQQQQQGLSQQASGQTGSAPSLGGEEGSGASQPWPPGGAPGEEAQGRMCPPEEHCYEAVGEKTWPKAVESDPAYATIDSHRKRDRPVSNTLKPKKKPSGQPQGPGRALTCENFYEAIGDAKQGANAMSTTTIFTFNDGMEMYVTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLE2</accession>
    <name>A0AAR2JLE2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557577.1">
        <property type="nucleotide sequence ID" value="XM_017702088.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017557578.1">
        <property type="nucleotide sequence ID" value="XM_017702089.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLE2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062453.1">
        <property type="protein sequence ID" value="ENSPNAP00000052835.1"/>
        <property type="gene ID" value="ENSPNAG00000031164.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429979"/>
    <dbReference type="GeneTree" id="ENSGT00940000166158"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000761">
        <property type="entry name" value="Zinc finger protein 449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF250">
        <property type="entry name" value="ZINC FINGER PROTEIN 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="122"/>
            <end position="149"/>
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    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="150"/>
            <end position="178"/>
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        <location>
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        <location>
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        <location>
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            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="210"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
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        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
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            <begin position="211"/>
            <end position="221"/>
        </location>
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            <begin position="290"/>
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        </location>
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        </location>
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            <begin position="393"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="406" mass="45873" checksum="5420D7BE8A604FC2" modified="2024-11-27" version="1">MDSDILKIEEIVMGGGETAAPADLNPEKAEQPYTTIIIQDPPPPPIIQSYQHESLQCFQCFITFCNSKAKERHMKKSHREEYKQQLQQCDTLFTCYVCDRTFPSSEELTQHQSTHNKEDKPFKCAHCQESFRTFSELTTHRRQVCPERQFGCKECGETFRSPALLRSHRLAQHPARQEAEDADDPTKTHRCGKCGRGFEEEAELLQHQENHAGNQHCNGSTPVKRRGRPPKAEPAVVVEKKQKRSKKEETEAAEEPGVSNHSEDVSTSPATAEAKAKTGARRGRPPKSTQEPKDEEDKAESKKMKAAPPPAREIPCPECDLTFPALAQLRAHKKEKHTQRKPHPCSECEESFNRPEQLEAHMTRAHTAGRYACPTCGKSFGRESNLKAHQQSHGKEEEKPAGRGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JN39</accession>
    <name>A0AAR2JN39_PYGNA</name>
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        <recommendedName>
            <fullName evidence="1">GPCR family 3 nine cysteines domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSPNAG00000034837.1"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Vomeronasal 2, receptor 82"/>
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        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
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        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
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    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
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    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GPCR family 3 nine cysteines" evidence="1">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="61" mass="6717" checksum="3CEC5D505AA1CA18" modified="2024-11-27" version="1">MIHFLKYHKKINVFQVPRSVCSESCPPGTRKAVQKGKPVCCYDCIPCAEGEISNKTGCASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JVN1</accession>
    <name>A0AAR2JVN1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Cadherin EGF LAG seven-pass G-type receptor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">CELSR2</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor that may have an important role in cell/cell signaling during nervous system formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000069414.1">
        <property type="protein sequence ID" value="ENSPNAP00000054312.1"/>
        <property type="gene ID" value="ENSPNAG00000020029.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157493"/>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120036">
        <property type="term" value="P:plasma membrane bounded cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000059">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000112">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000005">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000047">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000020">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000005">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000286">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
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        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
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        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 3"/>
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    </dbReference>
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        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
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        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        </source>
    </evidence>
    <sequence length="2948" mass="325457" checksum="3CAB2D5967CFF852" modified="2024-11-27" version="1">MKLGLFLAVLSRFSLLHGFVVHVGEDARPGTVITSAHGSASCTLEQVISPRFAQNFLRVDAPAGTASTSDALRCESLLTNPFAVHTVADCTTAGVTGVKFRHLVVSRYLVHVRGSSCARGPRRREKWDVEVVSLLQAPGPQVDCLEVGAGLFRVQEVLPGARTPGLSCNVTGTAISFAEEWLVVSAPLCLLDGSLLEFDLHCMEKVARGRRRSTHVRWHLGQGPFRQRYLEKLLWRTAEDSESGLLSRRKRSANANPQFQPPMYQVSVPENKAAGTSVVLLRAMDPDEGEAGRLDYFIEALFDSRSNNLFAVDTESGLVSTVEELDRETKDTHVFRVTAVDHGTPRRSAMATLTITVSDTNDHDPVFEQQDYKESVRENLEIGYEVLTVRATDGDAPINGNILYRILNSNGSNDVFEIDSRSGVIRTRGPVDRETVESYILLVEANDQGRVPGPRSATATVHIVVEDDNDNAPQFSEKRYIVQVPEDLAPNTEILQVTATDQDRGSNAVVHFSIMSGNTRGQFYIDAQTGKMDLVSQLDYEMNKEYTLRIRAQDGGRPPLSNISGLVTVQVQDINDNAPIFVSTPFQATVLENVPIGYSVIHIQAVDADSGDNSRLEYHLTETTPNFPFTVNNSTGWIIVAAELDRESLDFYNFGVEARDHGIPVMSSSASISMTILDVNDNNPEFTQKAYYMRLNEDAAVGTSVVTVSAVDQDINSVVTYQISSGNTRNRFSITSQSGGGLITLALPLDYKLERQYVLSVTASDGTRFDTAKVYVNVTDANTHRPVFQSSHYTVNINEDRPVATTVVVISATDEDTGENARITYFMDDSIPQFNIDPDTGAVTTQMELDYEDQVSYTLAITARDNGIPQKSDTTYLEILVNDVNDNSPRFLRDHYLGSIMEDVPIYTSVVQVSATDRDSGLNGRVFYTFQGGDDGDGDFIIESTSGIVRTLRRLDRENTPIYNLQAFAVDKGVPALKTSVDIQVTILDVNDNPPVFEKDEFDIYVEENSPIGLVVAHISATDPDEGSNAQIMYQIVEGNVPEVFQLDIFSGELTALMDLDYETRSEYIIVVQATSAPLVSRATVHIKLIDKNDNVPVLKNFQIIFNNYVTDRSNSFPTGVIGQIPAHDPDISDQLRYSFEAGNDLNLLILNQTTGQIRLSRALDNNRPLEASMRIAVSDGVHSVSAQCLLQVTIITDEMLSNSITLRLANTSQERFLSVLLAQFLEGVARVLSASREDVVVFNIQDDTDVNASILNVSLSVAVPAGGMRGRSDPGVEFFGSEELQERLYLNRSLLARISSQQVLPFDDNICLREPCENYMKCVSVLKFDSLAPFVASDTILFRPIHPIAGLRCRCPTGFTGDYCETEIDLCYSNPCGVHGVCRSHEGGFTCECLHDYTGERCEVSSRSGRCVEGVCKNGGTCVNLLVGGFRCECPLGGFEKPYCSISTRNFPPNSFITFKGLRQRFHFTLSLTFATREPDGLLLYNGRFNEKHDFIAMEIVNEQIQLTFSAGETKTTVSPFIQGGVSDGHWHTVQVHYYNKPILNQAGLPQGPSDHKVVVVTVDNCDTSMALRFGYVIGNYSCSAQGSQSGSKKSLDLTGPLLLGGVPRLPEDFPIQNRQFVGCMKDLRIDSRNMDMAAYIANNGTLPGCSAKRQFCINNPCLNGGTCVSLWGSFRCDCPLGFGGRNCEKVMSNPQRFQDNSMLLWSNLAVVVTVPWNLELMFRTRQPTSTILHVTTSQHHNFTVQLRDGSVVMMLQHGEDSAVSRVEDVSVDDGEWHHLQVELLGPAGGAAHSEVYKALLTIDHGLYSSSMEVDSKLKGVKLKTLSVGGVAVGNNKVLHGFHGCIQVQLLWSRGGAAAYPSSPRVLRVNVESGCSVPDPCSSNPCPPHSSCTHRWDSHTCTCHPGYYGNNCTDACALNPCEHQSACSRKLSSAHGYVCDCRSSYFGRYCEKKTDLPCPRGWWGHKTCGPCNCPIDKGFDSDCNKTSGECRCKDNHYRPAGSEVCLLCECYSVGSFSRVCERESGQCQCKPGVIGRQCDRCDNPFAEVSPNGCEVIYDSCPQAIEAGIWWPRTKFGLPAAVACPKGSIGTELQSWLSESCVSVLSLQSEQVGRNLSVLDFSNVQQTAALLFNATLHTHSYYGSDVKIAYRLTQGLLQHESSQQGFSLAATQDVHFNEHLLRVSSAMLASETRPHWEQIQQAEGGTAALLRHFEEYTHTLAQNMRRTYLSPFTIVTPNIVISIDRLDKMNFAGAKLPRYESLRGPRPLDLETAVTLPDSVFIPALEGKGHRHHSESVTEPARNQSGNRKRRHPDKREQDAVASVIIFHSLSTLLPQRYDTDKRSLRVPKRPVINTPVVSITVHDNEELVQHALDKPITVQFRLVTTEERSKPICVFWNHSISTGGPGGWSSKGCEVVFRNSTHISCQCYHMTSFAVLMDISHRENGEILPIKLVTWSTVGVTLFFLFLTVVFLTCLRAMYSNSTSIITNGALTLFVAELLFILGINQTDNPFVCTIIAILLQFFYMCVFSWLFLEGLHVYRMLSEVRDINYGPMRFYYLIGWGLPVPVYLLMFLLLLCVTFTGLAVGLDPEGYGNPDFCWLSLYDTLIWSLAGPIALVVAMNLFLYILASRASCSLRSNNYEKKQPPVYGLRTACVVLLLVTVTCLLALLSVNSDMILFHYLFAAFNCVQGPFIFFFRIVFNKEARGAMRYCCGKKRPEHVIKSKPSYTCSSSYADGRLYHLPYAESSVSLNGTLQSAKSQHSYVPFVLRYSHLFHETKPHPDDHDSDSDSDLSELEDDQSGSYASTHSSDSEDEEGQYLQEECWENLATSTAGGGGARLRVETVTHPEEKAEDYGKENTLPLLPSLHNPHTHPHKGILKKKPLSPIAERNGINRIHNQLSVNTSDPASSRGSSSSEGQGGGAKQDQLNGVAMSIKAGTVDDDSSGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JZE9</accession>
    <name>A0AAR2JZE9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ZC3H13</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSPNAT00000059899.1">
        <property type="protein sequence ID" value="ENSPNAP00000055637.1"/>
        <property type="gene ID" value="ENSPNAG00000030461.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234571"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01221">
        <property type="entry name" value="PH_ephexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000007">
        <property type="entry name" value="rho guanine nucleotide exchange factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047271">
        <property type="entry name" value="Ephexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047270">
        <property type="entry name" value="PH_ephexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845:SF2">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="1314"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="1530"/>
            <end position="1632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="646"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="798"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="898"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="964"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1017"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1134"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1233"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="502"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="554"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="688"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="735"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="746"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="769"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="837"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="923"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1031"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1065"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1239"/>
            <end position="1253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1653" mass="194133" checksum="E55770805BC55D62" modified="2024-11-27" version="1">MGTKRANWTIEDPSQLSPSPATPDRSYRSNEWISREARPQAGTKIRADRDREGEVRRAREMDRILEKAKGGRREEEQELREREMLTAFKARTGETEWRERERRRDEGRHSNGRIASVQEYSDHREREREKGDTFPRTGRQSNEREGRTRLSVDQSNERLMERGMGRLKELDQSESERERERVKKRDRDMQRYRETERQRENERARIREVSKRDGGRRLEKEDSDRKRGRGWDLGRRERRGERNAEVNDQQIMRLREVDRERERDRLGYRDIEKWLYSLQRDRIMEGESGNLSDREQRGERNSGWRRERHRHTKSEGDSDGEKEREVRRKETSEHLRYDQSKSERDIERERTRERWRERERCREMEWQRERDRNVGRERETDMLGNRERDKEWVIERHRERDMRDRDWVREERGPERQRGGVRDTQDRRRERESNERARRDQERYTQARPYASERVMDVNPERREMERRRSWEVGETTESSQTQSEAQRKVQVEAQSSGEWMSRSDVEHEQRMRERKDSFEDKERESQPESDREKKKKKINEEGQDATSGQGAGEAREGSEKEETETERDRDREKPESKQRQQRKMWLEPRSGRVRTESLEEEFRERERARERYAQRFKVGNASGEEDEMYNLRYERETEDRYRQLESAGEFTRDVDQENVEERRKGDEGRLDTPWQMETENVGVNMEGTDREEERGSGRSGDSEGEEGSEWELRGEGRERVTSADDGFVTVSSGGDEDEAEEDKFEDCKEFWDGGVREDLTRQSPTRTHQSEGEIETKMIEERSDRTVTVFCVVGQTLPRSGSNQLPHLDPMEQEDAGQHSAQELGGGSDVDQDVALPRDTEGTDESVKESYDLTLSEKEITNNHTTKGEKTECDEMNKEVTSAQNVELTGSVCAEKEIESHPQESSETLANAEDQEYAQKIMTDEESRKKEETHNTEGERKRYSAAPHVKWAKNVLKEILGSSDDGVLSMPEPGIQTEQPSDRAPAEAETQGESPASPLYGIVQKPCKHSIERKCIDPELLQCKPEDTESGSQLDTQGNKILIQVDRESETEEELDHLEQDKEMLEEDTDEEDNYDRLLPSPDLHSFSSEGEAEKEGSGKKDKKQKKKKTAWDFLSSSSFRDFGNEARARRKGFRKVVEKQSEDEEGEGIARDRRPRVFTTEEDEYDDLRHSFNESELRNFKARISRTRMRNSKFYNSQLYQQYNEVVQNREILHQSHSDASSVFQEIVTVPQSSSPCPSPPPARRPLPPLPSMLHPHSLSHTNSSRPLAIPQHTVTRPLSPRLSRSSSLLWQELPGVSNNPELQNLSEDERRLQEVRFEVVTSEASYCSSLDIVVEHFVKSKQLNSLLATQDKNWLFSRLGDVRAISNSFFSQLEERVESDVMHFTVCDIIIKHCPRFRAVYVPYLTNQSYQDKTYQRLMNENAGFRNIVEKLEHSPKCQRLPLRSFLILPFQRITRLKLLVQNIVKRTAPKTKDEAQAIKAMKLLEKMIQESNDSITQMKSIESLVSLNAKVDFECKTLPLISQSRRLVREGLVTELRDFSLKETERSVYIHLFNDYLLLSLPKEGGRFTVIDHTPVSELRAENCRVKLHSLQKNLFRLHMGHKVALLLRTETQADKLRWISAISRPHPEIDFGAAQGTCHFQSKMRLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K3K5</accession>
    <name>A0AAR2K3K5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K3K5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064905.1">
        <property type="protein sequence ID" value="ENSPNAP00000057039.1"/>
        <property type="gene ID" value="ENSPNAG00000031850.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271879"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="391"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="602" mass="69009" checksum="45D78EC85FF14F3A" modified="2024-11-27" version="1">MDIECIGLNVSLSPEMSFIVICLYRPPSSNSLFYEHLYSILKECDEKKELILMGDFNINWENKQDRKRLKEITDKFSLTQLVQGPTRVTKCSQTQIDLMFTNRQERITKSSNLLTGLSDHNFILVVRKLTKKRFKNIIKPKHYYQKIPKSEMHNLENALKQIDWLNILSCKDIDDNCDIFLSTINKVMSSFTRKVQSKPRQRETLPWLNDALLQQMKERDFALKRTLKTGLNNDRLIFTSLRNKVVRNIRKAKAEFFIKIIDNARGNGKMIWQNLNKLTGRNNDQRTKEYELKVNGTLIQDHDMIANTFNNFFINSIEQLTQSFSSKTIISTIIDDDIPIFRIMETTESAVLNIINSLRDSKAKDVYGLDTTFLKLHKGILASPIAKLINHSIRQGIFPSAWKSAVITPIFKAGDRTVVENYRPISILPAVSKVAEKWVVQQLTAHLNKSYTPLHSMQFGFRSNHSTETAICYFLEGVKSKLDKGGVIGAVFLDLKRAFDTVNHKVLLSKLSCFNFSIDSVKWMNSYISNRKQRVRIGKTQSTYLSCNTGVPQGSVLGPILFSLYINDLPSVCSSVNIQMYADDTVLYVHAKNKQQAASELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K909</accession>
    <name>A0AAR2K909_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">WD repeat domain phosphoinositide-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">WIPI2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K909"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042962.1">
        <property type="protein sequence ID" value="ENSPNAP00000058937.1"/>
        <property type="gene ID" value="ENSPNAG00000022384.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155537"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000145">
        <property type="entry name" value="WD repeat domain phosphoinositide-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="52883" checksum="40AAEE1E121D5C4A" modified="2024-11-27" version="1">MNLASQSGEAGCSQLLFANFNQDNTSLAVGTKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPSGLCALSISNDNCYLAYPGSATIGEVQVFDTVNLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMEGLYLSASSNTETVHIFKLETQREKPQEEPTTWTGYFGKVLMASTTYLPAQVSEMFTQGRAFATVRLPFSGHKNICALAIIQKIPRLLVAAADGYLYLYNLDPQEGGECTLMKQHKLDGSAEPANEILEQTAHERPLVAQTYSAAVAKGKNCFTSLYSFHWARNFSLKANAVKERFFQQIMFCILWLIEKNCKFWYASCSFSPQCVSFRLKKKKSQLININVLSIDQFGCQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K9G1</accession>
    <name>A0AAR2K9G1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microsomal glutathione S-transferase 1</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16438"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer; The trimer binds only one molecule of glutathione.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPEG family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017551253.1">
        <property type="nucleotide sequence ID" value="XM_017695764.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K9G1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062114.1">
        <property type="protein sequence ID" value="ENSPNAP00000060910.1"/>
        <property type="gene ID" value="ENSPNAG00000031321.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426350"/>
    <dbReference type="GeneTree" id="ENSGT00390000011980"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.550:FF:000002">
        <property type="entry name" value="Microsomal glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040162">
        <property type="entry name" value="MGST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689:SF6">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="152" mass="17459" checksum="9BAE66512A309AF1" modified="2024-11-27" version="1">MAHITDTEVFQAFSTYATIVILKMMLMGPLTGYFRVTRKAFANFEDTHMGKTIEEKKRMIRVNEDVERVRRCHQNDLENIIPFVVIGLLYTLTGPDLSTALLHFRLFVGSRFVHTVVYVMALPQPSRGLSWILGMVITFSMAYRILTTVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KCD4</accession>
    <name>A0AAR2KCD4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine in rRNA + S-adenosyl-L-methionine = N(6)-methyladenosine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58728"/>
            <dbReference type="Rhea" id="RHEA-COMP:15198"/>
            <dbReference type="Rhea" id="RHEA-COMP:15199"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KCD4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069220.1">
        <property type="protein sequence ID" value="ENSPNAP00000060102.1"/>
        <property type="gene ID" value="ENSPNAG00000006270.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183142"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034246">
        <property type="term" value="F:mitochondrial transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000209">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF13">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 13 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 14" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="17">
        <location>
            <begin position="248"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="82"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="569" mass="63113" checksum="AD16DEBC1CCD3ACC" modified="2024-11-27" version="1">MCVCVCVCVCVYVYMYIVLCILSSQNHKQYFISIEMMMMMIIIIIIVSFLVAGKGFHDDKCFGSFYFVTPTVASPESDAVSARLDRGSQQTAVCTRTERQTHAERPSPSSPSPVRSAGDHHQDLQLSMALCGGCRFLVLAVRSMHVSARSTPALAQAVRVFSSSSRVCSLDSFSMGQMKVPAGGVKTERTCPSVGSAQRNLSAVAVSLQGQRRPLCRYDLLDLGEVEENTRKALACKHLRRFIIDPSLAKLVAEHLSQDIEDGKAVIFECNPGPGVLTRTLLNCGAQRVVALESDKAFLPDLQALENNLDGQLEVVHCDFFKLDPIGHGSMKPPAMYSEKLFTDLGISEVPWTADVPVKVVGIFSQRNERNMLWKLVYALFERLSIFRYGRVELIMFISEKNYSKLVAQPGNNKSYQAVSALFQMSCNIELLHQEPWSSFVTTSRNGGLSIPKSTVVPNDHLCLVRITPRADLFTPTVTPFNSSTLVVMVKQCLAKRRGKLIDKLNSWSPGIGQDLLAQIGLSEDVLTGQVYPDQYKHLFELMEKSEQFTQSWLYSEVLENTKNTGCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHB4</accession>
    <name>A0AAR2KHB4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Exosome complex component RRP46</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Exosome component 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ribosomal RNA-processing protein 46</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">EXOSC5</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Homodimer. Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC3, EXOSC8, and EXOSC9. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6. Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. Exo-9 is formed by a hexameric base ring consisting of the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and a cap ring consisting of EXOSC1, EXOSC2 and EXOSC3. The RNA exosome complex associates with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with GTPBP1. Interacts with ZC3HAV1. Interacts with DDX17 only in the presence of ZC3HAV1 in an RNA-independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHB4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061314.1">
        <property type="protein sequence ID" value="ENSPNAP00000063680.1"/>
        <property type="gene ID" value="ENSPNAG00000030099.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153348"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11372">
        <property type="entry name" value="RNase_PH_RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000012">
        <property type="entry name" value="exosome complex component RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="14">
        <location>
            <begin position="12"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="15">
        <location>
            <begin position="136"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="220" mass="24097" checksum="B7CF03F44B10DAD3" modified="2024-11-27" version="1">FVEERSGVGSVLREYGSEQNLLSKPDGSASFIQGDTSVLAGVYGPAEVKVSKEIYDRATLEVVIQPKVGMASVRERAREQCVRETCEAALLSTLHPRSSFTLVLQEVHDDGSLLSCCLNAACMALMDAGLPMGRLFCGVTCAIDAESQIITDPTAQQEKESRALMTFAIDSKDCSVMMSLTKGSFSVKELQQCIAVSQKASERIFQFYRDSVQRRYSKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHQ9</accession>
    <name>A0AAR2KHQ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Phosphoacetylglucosamine mutase</fullName>
            <shortName evidence="13">PAGM</shortName>
            <ecNumber evidence="4 13">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PGM3</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 13">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, a sugar nucleotide critical to multiple glycosylation pathways including protein N- and O-glycosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13 16">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4 13"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHQ9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044842.1">
        <property type="protein sequence ID" value="ENSPNAP00000061989.1"/>
        <property type="gene ID" value="ENSPNAG00000023300.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000509"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000003">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000013">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000015">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000019">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 13 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="18">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="18">
        <location>
            <begin position="114"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="20">
        <location>
            <begin position="180"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="19">
        <location>
            <begin position="300"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="17">
        <location>
            <begin position="459"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="14">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="16">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="373"/>
            <end position="375"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="501"/>
            <end position="505"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR016408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="547" mass="60428" checksum="C35E4E872BA2EB2D" modified="2024-11-27" version="1">MDQLEKASQKSMLHPKPKGLMLQYGTAGFRTTAKHLDHVMFRMGLLATLRSRKTKSTIGVMVTASHNPEEDNGLKLIDPMGEMVTPSWEGYATQLANAEQEVLCSALKDIIEKETINMSETASVFVGRDTRPSSQRLSQAVLDGVSCLGGQIQDYGLVTTPQLHYMVRCRNTHGCYGTDTLQGYYQKLSQAFIELTKNARNHTDDQKRLLVDGANGIGALKVRELEACLQSELQMVLFNDGSNGKLNYLCGADYVKVQQKPPQGQLEYSPWCERCCSFDGDADRIVYYYNDSDGSFHLLDGDKIATLISTYLKELLTQAGLNLQIAVVQTAYANGSSTQYLEDIMKVTVSCTKTGVKHLHHAAQKFDIGVYFEANGHGTALFSEKAEKEIQQLVKDPNVSDEKKHAAKLLENTINLINQATGDAISDMLVIEAVLAIRGMTVQEWDSIYTDLPNRQLTVKVADRRMIDTADAERRAVTPAGLQDSIDALVKKYTKARAFVRPSGTEDLVRVYAEAETQESADVLAHEVSLAVYHMAGGVGEKPKPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KIX8</accession>
    <name>A0AAR2KIX8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MSH2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15 16">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15 16">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558710.1">
        <property type="nucleotide sequence ID" value="XM_017703221.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KIX8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086980.1">
        <property type="protein sequence ID" value="ENSPNAP00000062051.1"/>
        <property type="gene ID" value="ENSPNAG00000009400.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430621"/>
    <dbReference type="CTD" id="4436"/>
    <dbReference type="GeneTree" id="ENSGT00550000074867"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002204">
        <property type="term" value="P:somatic recombination of immunoglobulin genes involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03285">
        <property type="entry name" value="ABC_MSH2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000003">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000009">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000003">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000523">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032642">
        <property type="entry name" value="Msh2_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF35">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 15 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13 15 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="18">
        <location>
            <begin position="744"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="554"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="940" mass="104323" checksum="61165360A08FA07F" modified="2024-11-27" version="1">MAVQPKQNLSMDSASESGFINFFFSMPEKPDTTVRVFDRSDYYTVHGKDALFAAKEVFKTNGVIKHLGSGSRRLESVVLSKMNFESFVRDLLLVRQYRVEVYKNKSGSKSSKDHDWQIAFKASPGNLTQFEEILFGGGGLGEGAAGVVGIRLGTGGDGQRVVGVGYVDSTLRKMGVCEFPDNDQFSNLEALLVQIGPKECLLPGGDVAGDVGKLRQVIQRGGILLSDRKKAEFTTKDIVQDLNRLLKACKGETVSSAALPEMEKQIAVSCLAAVIKYLELLSDEANFGSFRMTTFDLNQYMRLDNAAVQALNLFQVSSDDPSGTHSLAGLLNKCRTPQGQRLVNQWLKQPLIDKNKIEERLDLVESFVEDSELRQSCQEDLLRRFPDLNRLAKKLQRQSANLQDCYRVYQAVGQLPGLVLALERHTGKHQILLNAAFISPFKDLISDFSKFQEMIETTLDMNQIEHHEFLVKPSFDPTLSELRENMDQLEKNMQATLNSAARELGLDAGKTVKLESNAQMGYYFRVTCKEEKNLRNNKKFTTLDVQKNGVRFTNSKLSSMNEEYIKNREEYEEAQNAIVKEIISIAAGYVEPLQTLNEVIAQLDAVVSFAVVSHAAPVPYVRPRILEKGSGRLILKGARHPCVEAQDDVAFIPNDVTFTRGEQMFHIITGPNMGGKSTYIRQVGVIVLMAQIGCFVPCDEAELSVVDCVLARVGAGDSQIKGVSTFMAEMLETAAILRSATEDSLIIIDELGRGTSTYDGFGLAWAISEYIATRLKSFCLFATHFHELTALAQQVPTVCNLHVTALTTDSTLTMLYRVRNGVCDQSFGIHVAELANFPKHVIANAREKALELEEFQDISRVDKEGVDAGPEAKRRCMEKQEGEKIIEAFLAKVKSLPVEGMTDEAIKAELCKLKAEVVIQNNSFVNEIVSRAENVKMVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJR1</accession>
    <name>A0AAR2KJR1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mortality factor 4-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MORF4L1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KJR1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055727.1">
        <property type="protein sequence ID" value="ENSPNAP00000062356.1"/>
        <property type="gene ID" value="ENSPNAG00000014280.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182965"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18983">
        <property type="entry name" value="CBD_MSL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.30:FF:000001">
        <property type="entry name" value="Mortality factor 4-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000024">
        <property type="entry name" value="Mortality factor 4-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF48">
        <property type="entry name" value="MORTALITY FACTOR 4 LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038133">
        <property type="entry name" value="HAT_Nua4_EAF3/MRG15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="13">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="339" mass="39177" checksum="97A4FAF95E408647" modified="2024-11-27" version="1">MAPKQDPKPKFQEGERVLCFHGPLLYEAKCVKINIKDKQVKYFIHYSGWNKNWDEWVPESRVLKYVDSNLQKQKELQKANQDHYVEGRMRGVAPSKKIAAVQQKNVDVGVQQCFCSCVFNLLYRKTKKNKQKTPGAGEGTSTGEMPHPPRKKRARVDPTVESEETFINRVEVKVKIPEELKPWLVDDWDLITRQKQLFHLPAKKNVDVVLEDYANYKKSRGTSDNKEYAVNEVVAGIREYFNVMLGTQLLYKFERPQYAEILANHPDTSMSQIYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLSYLQDFLKYLAKNSSSLFNASDYEVAPPEYHRKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNJ9</accession>
    <name>A0AAR2KNJ9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017571505.1">
        <property type="nucleotide sequence ID" value="XM_017716016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNJ9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077841.1">
        <property type="protein sequence ID" value="ENSPNAP00000065880.1"/>
        <property type="gene ID" value="ENSPNAG00000036366.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108438287"/>
    <dbReference type="GeneTree" id="ENSGT01120000271858"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF188">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="23"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="240"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="587"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="642" mass="73525" checksum="B02E5CB0EF8CE099" modified="2024-11-27" version="1">MASCIDPSHSRRKKSLTPPLSMSETLNLVVCGSDGAVKASISDFILGQRELSTESSSVCVRREGEVCGRLVTLVEMPALYNTQLSEEEVMQETLRCVSLCDPGVHAFLLMVHEGPLTDEDKGEMEKIQRIFGSRVKKHTILHFTTQSQQTVELSEASQFITETYKEQICVLDTSDISVLLQQIDRVVAQNNGSFYTTTMYLEAQVETQLRYKTEIEELKNTISYLRKQIKIQAQETAPGRGDLRIVLLGKTGVGKSASGNTILDKEVFRELLSSLSVTSICQKESAEIGQRWITVIDTPGLCDTNIPEIQVRKEITKCITMATPGPHVFLLVLSLGRFTQEEKEAVKIIQELFGEESRRYTMVLFTRGDDLRNTCIKEFIHSDNRLQNIIHQCGDRYHVFSNRKTEDRSQVTDLLEKIDSMVAVNGGSCYTNEMFQQVEEALQEEQERILKEKDDAIERKKEELKAKHEAEMEKLKRIMQEERRNLASERREKETEFLKREAEIKMETNEELKKEVDKELGEQQEAFNKEMERREQDHEKQMHKHLEYLKEQYEREMENIRKKGEEKARQKAEEEFCSKLEEEVAKAREEGKKEGYEEARRDAEPKQSHAGRLSHVLRKKAGPVGGAVGGAFGSLLDWIGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KS20</accession>
    <name>A0AAR2KS20_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Serine/threonine-protein kinase receptor</fullName>
            <ecNumber evidence="17">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KS20"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083701.1">
        <property type="protein sequence ID" value="ENSPNAP00000065197.1"/>
        <property type="gene ID" value="ENSPNAG00000027259.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156210"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048179">
        <property type="term" value="C:activin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048185">
        <property type="term" value="F:activin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017002">
        <property type="term" value="F:activin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23632">
        <property type="entry name" value="TFP_LU_ECD_ACVR2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000099">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000094">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF70">
        <property type="entry name" value="ACTIVIN RECEPTOR TYPE-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00653">
        <property type="entry name" value="ACTIVIN2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043882572" description="Serine/threonine-protein kinase receptor" evidence="18">
        <location>
            <begin position="25"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="190"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="512" mass="58057" checksum="EF50162CC18084E7" modified="2024-11-27" version="1">FGAVQLQSVDLLIVMCLCVGPSHGEVETRECLYYNINWEIEKTNQSGVERCEGEKDKRSHCYASWRNNSGTIELVKKGCWLDDFNCYDRQECVATEENPQVFFCCCEGSFCNERFTHLPDVSGPVIEPPPATLSLLNVLVYSLLPVIMLSMAMLLAFWMYRHRKPPYGHVDINEDLGPAPPSPLVGLKPLQLLEIKARGRFGCVWKAQMMNEYVAVKIFPIQDKQSWQNERDMFSTPGMKHENLLRYIAAEKRGTSLEMELWLITEFHEKGSLTDYLKGNVVSWNDLCHIAETMARGLAYLHEDVQRYKGEGPKPAIAHRDFKSKNVMLKNDLTAVIGDFGLAVKFEPGKPPGDTHGQVGTRRYMAPEVLEGAINFQRDAFLRIDMYAMGLVLWELVSRCTAADGPVDEYMLPFEEEIGQHPSLEDLQDVVVHKKLRPVLKDCWLKHTGLEQMCETIEECWDHDAEARLSAGCVEERISQIRRLTSAPTSDCLVSMVTSVTNVDLPPKESSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L2B2</accession>
    <name>A0AAR2L2B2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L2B2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061373.1">
        <property type="protein sequence ID" value="ENSPNAP00000068516.1"/>
        <property type="gene ID" value="ENSPNAG00000033166.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163096"/>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="64682" checksum="00B70029AA569387" modified="2024-11-27" version="1">MSKKRKIDSECRIFSQQWTSDDFFVQCKEKAVCLICQESISVFKSDKIAKLKHVLTTQQNSFLKQKQLNISSVRASFQLAKLIAKSGKPFTEGEFVKQCLTAVVMEMCPEKTDSFSAVSLSAPTITRRVEEMGDNLHLQLQTKTNSLCCFSLALDESNDVHDMAQLLIFIRGINEKFKVTEELAALQSIKGTTTEEDIYEKVCQTVADLGLDWAKLTSVTTDGAPSMVGSARGVIVRINREMSERRHPRPITIHCLIHQQALCCKAVKWDSVMKVVVSCVNFIKANALNHRQFQELLSELDAAYGDVLYHTEVRWLSRGRVLRRFNDLLPEINTFMRSKSKVVPELTDAEWKWHLSFLTDVTELLNSLNVQLQGKGRLICDMHSYVKAFEVKLGLLLKHVKERNFCHLPTTQKLAAEEPSQSFPTEKCAEALDILQSEFQVRFRELHIHTKDIRLFQNPFAADIDCVDPIYQLELAKLQNVDSLKDAFTTYICEQTFSRLKHIKSPIRSRLTDEHLHHLLRLGVTNMEPDIGCLLANVKHSSATCTAQRIDDTIDTCRNKTKKTLISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L654</accession>
    <name>A0AAR2L654_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actinin alpha 3b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L654"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062924.1">
        <property type="protein sequence ID" value="ENSPNAP00000072078.1"/>
        <property type="gene ID" value="ENSPNAG00000012529.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153968"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000156">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="41"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="154"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="756"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="794"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="440"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="674"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="916" mass="105434" checksum="92AAACC09F700484" modified="2024-11-27" version="1">MTAVETQVQYQSSYMMTSTEEYMIQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDKGKMRFHKIANVNKALDFICSKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHISWKDGLALCALIHRHRPDLIDYSKLRKDDPIGNLNTAFEVAEKFLDIPRMLDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMQEYEKLASELLEWIRRTIPWLENRVPEQTMPAMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDIANAWKGLEQVEKGYEEWLLTEIRRLERLDHLAEKFKQKCSLHETWTAGKEELLSLKDYESASLMEIRALMRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAATINARCQGICDQWDNLGTLTQKRRDSLERVEKLWETIDQLFLEFAKRAAPFNNWMDGAMEDLQDMFIVHSIEEIQSLITAHDQFKATLPEADKERMATLGIQNEILKIAQTYGIKLTGENPYTNITPQDISNKWEAVKQLVPMRDQMLQEEVARQQSNERLRRQFAAQANIIGPWIQTKMEEISHVSIDIAGSLEEQMSNLKQYEQNIINYKSNIDKLEGDHQLSQEGLIFDNKHTNYTMEHVRVGWEQLLTTIARTINEVENQILTRDAKGISQEQLNEFRASFNHFDRKRNGMMDPDDFRACLISMGYDLVSGEVEFARIMTLVDPNNTGAVTFQAFIDFMTRETAETDTAEQVMASFKILASDKVSFLIVHSAYLDCFSAIHYSGGAETGAASRAGRVLHQPCSGLHLLLQLAPLLLLVTSSLMFPTKEAQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L683</accession>
    <name>A0AAR2L683_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative nuclease HARBI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Harbinger transposase-derived nuclease</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transposase-derived protein that may have nuclease activity. Does not have transposase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L683"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075530.1">
        <property type="protein sequence ID" value="ENSPNAP00000069881.1"/>
        <property type="gene ID" value="ENSPNAG00000032434.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154348"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026103">
        <property type="entry name" value="HARBI1_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF267">
        <property type="entry name" value="NUCLEASE HARBI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02086">
        <property type="entry name" value="PUTNUCHARBI1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="13">
        <location>
            <begin position="148"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="335" mass="37654" checksum="39FE52CDB193C4A1" modified="2024-11-27" version="1">MAFLALLEDLASRQIRRQRIFRDHEDFFANDDDWLMSRFRFPRAILLTLCAELGPDLERPTLQNRAIPVHLQVLTTLGFLATGCFQRELADRSGISQPSLSSMMSGVLSGIIKLTGRYIRFPYTLSEQADIKVQFAAVAGFPNVIGAIDCTHVAIRAPSQNEAAFINRKQIHSINVQVICDATMTLTNVVARWPGSTHDSFILRNSSVGTRLENGAVRDGWLLGDSGYPLKRWLLTPFPNPQSTEERRYSDVPTLTRSAVERTIGLLKGRWRCLDASGLHLRACAVLHNMAQQNGLPLIMTDQQANDPEPDPHVYPPDARNIQAVRLREDVMRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L6R9</accession>
    <name>A0AAR2L6R9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actinin, alpha 2b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L6R9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046616.1">
        <property type="protein sequence ID" value="ENSPNAP00000072250.1"/>
        <property type="gene ID" value="ENSPNAG00000023103.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153968"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="869"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="38"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="151"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="816"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="920"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="933"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="943" mass="108261" checksum="E61F4A42AEF31C1A" modified="2024-11-27" version="1">MTQIENMLPYDNGYDQDEYMMQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGVQIENIEEDFRNGLKLMLLLEVISGERLSKPDRGKMRFHKIANVNKALDFITSKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHVSWKDGLAFCALIHRHRPDLIDYAKLNKDDALGNLNLAFDIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKILGVNQENEKLMEEYERLASELLEWIRRTTPWLENRVSEKTMALMQRKLEDFRDYRRMHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIASAWQGLEQAEKGYEEWLLTEIRKLQRVDHLAEKFRQKATSHETWASGKEEILTQKDYESASLMEIRALLRKHEAFESDLSAHQDRVEQIAAIAQELNELDYHDVAAVNQRCQSICDVWDQLGTLTQKRREALERTEKLLETVEQLFLEYAKRSAPFNSWLEGAMEDLQDMFIVHTIEEVQSLLAAHEQFKSTLPEADAERQAILGIQQEVQKIFHSYGIHGNLTNPYSTISPEEILSKWEKVKKLVPQRDGALQEELARQHGNERLRRQFAAQANLIGPWIQTRMEEIGRCSLEIGGTLEDQMTQLKQYEHVIISYKPNIDKLEGDHQLIQESLIFDNKHTNYTMEHIRVGWELLLTTVARTINDIETQILTRDAKGISQEQMNEFRSSFNHFDRVLFSDQSLSPTTTKISELQQQLQPRSEKKNGAMDTDDFRACLISMGYDLGEVEFARIMMLVDPNATGVVTFQSFIDFMTRETADTDTSEQVVASFRILAADKVSVCLCVMLFTAVINSIFAVVSFSSKSCPGTRLSTASPECHPILAPGLSQGPWTIPPSPPPSTERVTFNLTNGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L6T2</accession>
    <name>A0AAR2L6T2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleoporin NDC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Transmembrane protein 48</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NDC1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the nuclear pore complex (NPC), which plays a key role in de novo assembly and insertion of NPC in the nuclear envelope. Required for NPC and nuclear envelope assembly, possibly by forming a link between the nuclear envelope membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561804.2">
        <property type="nucleotide sequence ID" value="XM_017706315.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L6T2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080329.1">
        <property type="protein sequence ID" value="ENSPNAP00000070409.1"/>
        <property type="gene ID" value="ENSPNAG00000011490.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432480"/>
    <dbReference type="GeneTree" id="ENSGT00390000014590"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019049">
        <property type="entry name" value="Nucleoporin_prot_Ndc1/Nup"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269:SF6">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="380"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="507"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="678" mass="75195" checksum="3E9CB388C5D36E81" modified="2024-11-27" version="1">MPSCAESSWFARKVVRWRATASVSWSVLLLPATTMLFIFLIRFQLFHPIQWTSECLGLLTAASTIFSLLLLSGVVLVIGFFNLEYYTVVPSIPCSRVALLAKVLHPRQCVHSLVHCTVGMVVMWCASVMAGGRYRTLGFPCTHSESSSGAHDACLNEYHLFFLLAGAFMGYSHSFLGVVQNMNYVSFQIIQQYKYLRFKGSLPWVVKCSAVQSLYSVRNYVALYFFFGHIPRAWISNTLNLQIDSSLHSLDSLAGLLDFSLLYHLWISGTFLLFTWYITVLLFRIYVTESYSFPVQSTFAEDAEQCLPKVLAEKNTLIMKFLALQDLALLSQHSPSRRQEVFSLSQPGGHPHNWNAISKECLALLNELMQRLVAHHDAVASNGRAKPPSASSDARSSSETSVSSVVMSGTEDLPQTPRPSVLMRTPGSVFLKSSNRSVGSPVTAPFTPDLDSPFSTPALRRLAPQPGPQQATWFSSVQSPHVMRRGPKLWSASTDSQTNGSATASPAPAPSSPPPEQKPSFIAQWLQNRKEQVRGFLAKRVLVVYLFNKLPEASSQALFADGQAHIWALEGLSHLVASSFSEDKYGVVQTTLPSILGSMLSLQEAVDRHFKLPHASSKPVKTSCSLGDSTYKTLRFALRAALKTAIYRITSTFGEHLNAVCISAEHQKRLQQFMEFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBB6</accession>
    <name>A0AAR2LBB6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acylphosphatase</fullName>
            <ecNumber evidence="2">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ACYP2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBB6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058051.1">
        <property type="protein sequence ID" value="ENSPNAP00000071681.1"/>
        <property type="gene ID" value="ENSPNAG00000036063.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011103"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000011">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10029:SF23">
        <property type="entry name" value="ACYLPHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="8">
        <location>
            <begin position="58"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="148" mass="16395" checksum="36C3FE8D85B33614" modified="2024-11-27" version="1">MAQFYTVTLHHQHHQTVCLSVYLSISLSIYVFVCLSISVLALLIMSGDTGSSGSNLCSVDFEIYGHVQGTCLIPFTEREGLTLGLVGWVKNTNRGTVVGQVQGPRQQVHEMKVWLSKEGSPSSRITRAVFKNERDISKLELSGFSTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LD19</accession>
    <name>A0AAR2LD19_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RPS7</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017537939.1">
        <property type="nucleotide sequence ID" value="XM_017682450.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LD19"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054026.1">
        <property type="protein sequence ID" value="ENSPNAP00000072557.1"/>
        <property type="gene ID" value="ENSPNAG00000022404.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411068"/>
    <dbReference type="CTD" id="6201"/>
    <dbReference type="GeneTree" id="ENSGT00390000014122"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000554">
        <property type="entry name" value="Ribosomal_eS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047861">
        <property type="entry name" value="Ribosomal_eS7_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01251">
        <property type="entry name" value="Ribosomal_S7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00948">
        <property type="entry name" value="RIBOSOMAL_S7E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000072557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="194" mass="22153" checksum="FD1E956C43C4162F" modified="2024-11-27" version="1">MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGSRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRILPKPTRKSHTKNKQKRPRSRTLTSVHDAILEDLVFPSEIVGKRIRVKLDSSRLIKVHLDKAQQNNVEHKVETFAGVYKKLTGKDVVFEFPEFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LFV6</accession>
    <name>A0AAR2LFV6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 65</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TMEM65</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017573887.1">
        <property type="nucleotide sequence ID" value="XM_017718398.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LFV6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000056157.1">
        <property type="protein sequence ID" value="ENSPNAP00000073599.1"/>
        <property type="gene ID" value="ENSPNAG00000010787.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439799"/>
    <dbReference type="CTD" id="157378"/>
    <dbReference type="GeneTree" id="ENSGT00390000017802"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003231">
        <property type="term" value="P:cardiac ventricle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903779">
        <property type="term" value="P:regulation of cardiac conduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019537">
        <property type="entry name" value="TMEM65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21706">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21706:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10507">
        <property type="entry name" value="TMEM65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="27488" checksum="89BDC18BC39CF721" modified="2024-11-27" version="1">MLRSCLLRAVQPCMPGLRGPYGCTSPRHPALLSLLQRCSLGTHKHKEKLELLNSPKGAKDFIYSLHPTERSCLLKELQSFESMAIAQESAPFRRGLSRDKVQAEVTEEKLEALPPNSSQLRYVLLHNAIPFVGFGFLDNAIMIAAGTQIELSIGVTFGISTMAAAALGNLVSDLAGLGLAGYVEALAARLGMQIPDLTPKQVDMWQTRLSSHMGKAIGVTIGCLLGMFPLFFLSDEDEEETMDTPLTTPTDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LHE1</accession>
    <name>A0AAR2LHE1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gem (nuclear organelle) associated protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">GEMIN8</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017565491.1">
        <property type="nucleotide sequence ID" value="XM_017710002.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017565492.1">
        <property type="nucleotide sequence ID" value="XM_017710003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LHE1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048174.1">
        <property type="protein sequence ID" value="ENSPNAP00000075988.1"/>
        <property type="gene ID" value="ENSPNAG00000034787.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108434681"/>
    <dbReference type="CTD" id="54960"/>
    <dbReference type="GeneTree" id="ENSGT00390000013608"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034754">
        <property type="entry name" value="GEMIN8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16238">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16238:SF7">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15348">
        <property type="entry name" value="GEMIN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="25142" checksum="C73FC92F4E2E8F20" modified="2024-11-27" version="1">MDAWYAHPVYARYWQHYQQAMSWHHRHRRAYSKALQAAYGPALYPVFPTVTPRYADWHGEERDDRAQGRRRRWGREEEEEQDDPENEEESESSEESEIECNVSNMDISAELRQFFAQTERHREELKKQQQMEAERQEAYVLADQDLHRVSWRSAQPPAERPGERRSAEMKKLYGKDAAKIQGMEAAMQLNFDRNCDRKQPKYWPVIPLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJE8</accession>
    <name>A0AAR2LJE8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="10">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJE8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044833.1">
        <property type="protein sequence ID" value="ENSPNAP00000074451.1"/>
        <property type="gene ID" value="ENSPNAG00000022113.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009048"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000003">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000002">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000003">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000013">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF13">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="12">
        <location>
            <begin position="52"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1002"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1004"/>
            <end position="1016"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1188" mass="135413" checksum="8F9CD63183F9F60B" modified="2024-11-27" version="1">MSITSITTFYSESDVLIIRYEEIHHVKLKKKRTEESCLIKTKCLKFASERQISVGICSMMKKSNSKPMTQIMERLCKFEYISVVIFPEDVILNEPVEKWPVCDCLVSFHSKGFPLDKAVSYAKLRNPLLINDLNMQYFIQDRREVYRILQEEGIDLPRYAVLNRDPDRPEECNLVEGEDHVEVNGEVFHKPFVEKPVSAEDHNVYIYYPTSAGGGSQRLFRKIGSRSSVYSPESTVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEIRYPVMLTAMEKLVARKVCLAFKQTVCGFDLLRANGHSFVCDVNGFSFVKNSMKYYDDCAKVLGNMIMRELAPQFHIPWSIPMEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRNALFFDLFEKYGGYKTGKLKLKKPKQLQEVLDIARQLLAELGQHNDCEIEEKKSKLEQLKTVLEMYGHFSGINRKVQLTYLPNGQPKASSEEEDSLKEGPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDLKIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLTDCQQRVKARLHEIMQKNQDFTEEAYQKLAPTGSPSLVNSMKIIGNPVKTCDQVYALIQSLTCQIRKRLEDPKSANLQLYHSETLELMLQRWSKLERDFRMKSGRYDISKIPDIYDCVKYDTQHNSSLSLDNMLELFRLSRALADIIIPQEYGISKLEKLDIASAYCLPLIKKIQLDLQRTHEDEAVNKLHPLYSRGVMSPGRHVRTRLYFTSESHVHSLLSIFRYGGLLNEEKDEQWKQAMDYLSAVTELNYMTQIVIMLYEDNNKDPSSEERFHVELHFSPGVKGCQDDENAPMGFGFRPASVENQEKQEDLGSLEDLSKDEPDRAILTEPLSIHRKSPLIRSRKAGSMEVLLFSSLFTGLFSASFRGVSSSAPNLRELARAQRKKTTSPRRWSKRDEAAEEEQQVAELLGGLSADPALTHHLHRCSYHFRLFTAWLRSHSEPPDCFSGFEGCSMVPSIYPLETLHNSLSLKQVDEFLNKVCESRSETLTKTLKFLQCLEKVFTPLEVFHILSCYAHKLTCILLGSYVIDQHKVASNCEVKRKFYVVFKLKKKIKNLESVAFTQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LKD5</accession>
    <name>A0AAR2LKD5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Upstream-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UBP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the grh/CP2 family. CP2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LKD5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046604.1">
        <property type="protein sequence ID" value="ENSPNAP00000077010.1"/>
        <property type="gene ID" value="ENSPNAG00000027222.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156148"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000036">
        <property type="entry name" value="upstream-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF13">
        <property type="entry name" value="UPSTREAM-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25416">
        <property type="entry name" value="GRHL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="11">
        <location>
            <begin position="71"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="247"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="332"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="560" mass="63194" checksum="CCB05D4B376C03A2" modified="2024-11-27" version="1">MRGFPSAGLCDNCFHSDYDRKGLCLACSHECCTDKVLSLSLSLSLSLSSDVLALPIFKQEDGSVPVESDSKNPPFQYVLCTATSPAVKLHEETVTYLNQGQSYEMRLLDNRKKGEVPELNNKTVKSIVRVVFHDRRLQYMEHQQLEGWKWNRPNDRLLDIDIPMCVGITEPRTHGSQLNAVEFLWDVSKRASVFVQVHCISTEFTPRKHGGEKGVPFRIQIDTFTQDENGEYTQHLHSASCQIKVFKPKGADRKQKTDREKMEKRSAQEKEKYQPSYDTTILSETRLEPVMEEAVEQELKKSSKRTLPADCGDSTAKSKRGSCSPWPENAYVNPNTAATPTFTSNTHSYSSSNAVPDSETSSPNHQGDAATHVAMESLSPTASMQDVQQWLHKNRFNSYSRLFTHFSGSDLLKLTREDLVQICGPADGIRLYNALKSRVVRPRLTVYVCQEVSHGESPLLERRCHSKNGEHGSPTAIHVYHALYLEEMTACELTRKISNVLSLPLTHINQVYRQGPTGIHILLSDQMVSNFPDESCFTVSTLKGNELIFYMKVLDFIHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLM6</accession>
    <name>A0AAR2LLM6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial glutathione transporter SLC25A40</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 25 member 40</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>glutathione(in) = glutathione(out)</text>
            <dbReference type="Rhea" id="RHEA:74819"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575294.2">
        <property type="nucleotide sequence ID" value="XM_017719805.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLM6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077245.1">
        <property type="protein sequence ID" value="ENSPNAP00000077445.1"/>
        <property type="gene ID" value="ENSPNAG00000015789.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440749"/>
    <dbReference type="CTD" id="55972"/>
    <dbReference type="GeneTree" id="ENSGT00940000165993"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF5">
        <property type="entry name" value="MITOCHONDRIAL GLUTATHIONE TRANSPORTER SLC25A40-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="39"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="165"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="258"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="371" mass="41319" checksum="478BBF75357965DD" modified="2024-11-27" version="1">MKSDRNKIITQLFTERERETELRFVKMTSQSSQVSKDSITPLQQMMASCSGAILTSLFVTPLDVIKIRLQAQTSPFPKGKCFVYCNGLMDHICVCVNENGKAWYKPRGHFKGTLDAFAKIVRMEGIKSLWSGLPPTLVMAVPATVIYFTSYDQLCNALRLRMDEQADKAPLLAGAIARVGSATLISPLELIRTKLQSERQSYRELSAVIRSAVHTEGWLALWRGLGPTLLRDVPFSAMYWYNYEKGKSLLCRYKSSEPTFTITFIAGALSGSVASIVTLPFDVVKTRRQVELGELQARNLSAQGSSTYTVMKRIVGESGIRGLFAGFMPRLIKVAPACAIMISTYEFGKAFFRKSNEEKQTQVLALQTSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LNA6</accession>
    <name>A0AAR2LNA6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Contactin 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CNTN1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. Contactin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LNA6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051247.1">
        <property type="protein sequence ID" value="ENSPNAP00000075826.1"/>
        <property type="gene ID" value="ENSPNAG00000018336.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428400"/>
    <dbReference type="CTD" id="353150"/>
    <dbReference type="GeneTree" id="ENSGT00940000155915"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000035">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000044">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000047">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000052">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000054">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000064">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF10">
        <property type="entry name" value="CONTACTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043938833" description="Contactin 1" evidence="15">
        <location>
            <begin position="25"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1014"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="39"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="137"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="242"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="332"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="414"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="506"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="620"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="724"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="826"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="919"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="701"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1034" mass="114482" checksum="0ABE4FBA2A722586" modified="2024-11-27" version="1">MRHLTSLLLAVSLTLSSSPAVVCGDPSGIFGDDSMGYGPVFEEQPVDTIYPEESPEDKIIMSCRARASPPATYKWRLNSWEIEVQDGSDDHYTVVGGNLAISNPDKSKHAGNYSCLATNEFGTVISREASVQFGYLDMFSTEEREAVYVKEGQGVVLLCAPPPHFPNDLSFRWMLNEFPIFIRLDKRRFVSQTTGNLYISKVEASDSGNYSCFVSSPSIAKSVFSKFIPLVPLAERPIRKYPADIKVKFPDTYALVDQNITLECFALGNPIPQIRWKKVDGALPAHHEISTVGGLLHLFNVQFEDEGIYECEALNSKGKDWHKAHLYVEAPPEWVEHISSSEKGIGSDYTMSCLASGKPKPQIHFLKNGQMYGKHELRFTDMTFDDSGMYQCIAENRHGVIYANAELQVFASPPSFELNPLKQRVLGAKNGHVVIECKPRAAPRPTFSWSKGTELLSNSSRIFIWEDGSLEIRNVTKSDEGKYTCFAENDRGKANSTGSLLVTDATKITLAPSNADVSVGENTKMQCVASHDSSLDLTFIWTLNDHVLDFDKEEEYYQYVMDGQAGISSCELLIKNTQMKHAGRYTCMAQTPVDNVTSSADLVVRGVSLCNPLICGPPGPPGGVRVEEIGPKSVRLLWSQGSDNLSPISKYTIQYRETRTEEAWKDAVTSPPNVEGNAETATVIDLIPWTEYEFRVIATNTLGTGEPSDPSPKTRTQDAVPVVAPSDVGGGGGTSRELTITWTPVQPQYYYGSNFGYIIAFKPEDGFQWRKVTVADPEARRYVHKDPSIPPATKFDVKVKAFNSKGEGPYSLTAAIYSAQDVPSEAPTIVEARTLSATEAIIWWQPVLQQTVEGYQVRYWRKSMENEATAQRISNRENQTRLDNMKPDSHYVIEVRAFNGAGYGPPSQRYEIHTKKAPPSRPPKIINTKMNPSRTSVNIAWEQVEPLVNESTVQCYKVLYRQEGKSTGVLYTTDKQYIDLPMRKDGDYLVEVRAHSEGGDGAVAQVRISGGGVLVAQSLSICVLLLVAVLSLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LTA0</accession>
    <name>A0AAR2LTA0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ras homolog family member Q</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RHOQ</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558623.1">
        <property type="nucleotide sequence ID" value="XM_017703134.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LTA0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050288.1">
        <property type="protein sequence ID" value="ENSPNAP00000079828.1"/>
        <property type="gene ID" value="ENSPNAG00000037738.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430567"/>
    <dbReference type="CTD" id="23433"/>
    <dbReference type="GeneTree" id="ENSGT00940000155970"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000438">
        <property type="entry name" value="Rho-related GTP-binding protein RhoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27650" checksum="55588030D441ACA9" modified="2024-11-27" version="1">MSGVLSCRLASHRSRQSGALVTPEEEEEGEDDDDDEEDDDDNFACVMANGTGTIMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYLLGLYDTAGQEDYDRLRPLSYPMTDVFLICFSVVNPASFQNVREEWVPELQEYAPNVPYLLIGTQIDLRDDPKTITKLNDVKEKPIVTEQGQKLAKEIGACCYVECSALTQKGLKTVFDEAIIAILAPKKGALKRRLGPRCINCCLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LUW1</accession>
    <name>A0AAR2LUW1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Syntaxin-binding protein 5-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568483.1">
        <property type="nucleotide sequence ID" value="XM_017712994.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LUW1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065364.1">
        <property type="protein sequence ID" value="ENSPNAP00000078442.1"/>
        <property type="gene ID" value="ENSPNAG00000009133.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436475"/>
    <dbReference type="CTD" id="556789"/>
    <dbReference type="GeneTree" id="ENSGT00950000182906"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15893">
        <property type="entry name" value="R-SNARE_STXBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000521">
        <property type="entry name" value="syntaxin-binding protein 5-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000664">
        <property type="entry name" value="Lethal2_giant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013577">
        <property type="entry name" value="LLGL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF22">
        <property type="entry name" value="SYNTAXIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08366">
        <property type="entry name" value="LLGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00962">
        <property type="entry name" value="LETHAL2GIANT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="230"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="17">
        <location>
            <begin position="1078"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="551"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="672"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="873"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1143" mass="126219" checksum="C20152C420E6453A" modified="2024-11-27" version="1">MKKFNIRKVLDGLTAVSSPSSSPQTGAKENDFIIPETLQSEHFQLCKTVRHGFPYQPSSMAFDPVQKILAIGTQSGALRLFGRPGVECYCQHESGAAVIQLQFLINEGALVSALADDSIHLWNLRQKRPAVLHSLKFNRERITFCHLPFQSKWLYVGTERGNIHIVNVESFTLSGYTIMWNKAIELSSKAHPGPVVHISDNPMDEGKLIIGFESGIVVLWDLKSKKADYRYTYDEAIHSVAWHHEGKQFICSHSDGTLTIWNIKSPAKPAQTITPHGKQPKDGKKPEPCKPILKVEYKTTRAGDPYMILSGGLSYDTVGRRPCLTVMHGKSTAVLEMDYPIVDFLTLCETPYPNDFQEPYAVVVLLEKDLVVIDLAQNGYPIFENPYPLTIHESPVTCCEYVADCPVDLIPALYSVGSRQKRQGYSKKEWPISGGNWGQGTQSYPEIIITGHADGSVKFWDASAIMLQVLYKLKTAKVFEKTRSKEEKPNTEIVDEDPFAIQLMSWCPDSRMLCVAGVSAHVIIYRFSKQEITTEVVQLLEVRLQYELNDLESPEGGGYQTPAPPTPSTSPSPQTSGPQTHHSTSSNSSDGIRDNVPCLKVRNSPLKQSPGYQVELVVQLVWVSGEPPQQISSLAINSSYGLVVFGNTNGLAVVDYLQKTVLLNLSTVELYGSNDPYQRQPRSPRKSRQPSGGLCDINEGSATSEDRCKSPTSVLMSNHQQFYNGGGRGAKMSRKLSLPTELKPDLVLLSVCPPRAALPLDNRDNSFSRSRSSSVTSIDKEAREAITSFYFCETYTRKTDSTLTPALWVGTSLGTVLSISLSMPPAGEQRLLQPVIVSPSGTLVRLKGSILRMAFLDATGSFLPPPSELWYEPNAPANGEERERVRKRRPVSVSPSTSQELNEHQYAVVCSEKQAKVIALPSQTCIFKHNITETSFVLRTDVVQTVPGICVACFCANGHIMTLSLPGLRPLLDVNYLPLTDMRIARTFCFTNLGQAMYLTSPTEIQRITYSQDVCENLQEMLGELFTPVETPEAPNRGFFKGLFGGGAQSLDREELFGETGAGKASRSLAQHIPGPGGIEGMKGAASGIVGDLARARIALDERGQKLSEVEERTAAMMASADSFSKHAHEMMLKCKDKKWYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LXE7</accession>
    <name>A0AAR2LXE7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">R3H domain containing 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">R3HDM1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LXE7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079997.1">
        <property type="protein sequence ID" value="ENSPNAP00000078991.1"/>
        <property type="gene ID" value="ENSPNAG00000005436.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156095"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000001">
        <property type="entry name" value="R3H domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051937">
        <property type="entry name" value="R3H_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21 RELATED R3H DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672:SF12">
        <property type="entry name" value="R3H DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="3">
        <location>
            <begin position="132"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ" evidence="4">
        <location>
            <begin position="196"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="472"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="649"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="781"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="352"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="659"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="784"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="850"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="869"/>
            <end position="881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1074" mass="117575" checksum="A0675854DB17B802" modified="2024-11-27" version="1">MRMSDTVNVKETSETMRGLEVEESNSTPGGNSLTKSESREQIEPDGDENCCDDKSETQDEIQIKLSQSFDKEDAPASKNEDDKDKSGDKLEKLEKMPRKMLSRDSSQEYTDSTGIDLHEFLVNTLKNNPRDRMMLLKLEQDILDFISNNESQRRKFPPMTSYHRMLLHRVAAYFGLDHNIDQTGKCVIINKTSNTRIPDQKFSEHIKDDKTDDFQKRYILKRDNSSMDKDDNMMRMRLKEDRRSKSIEEREEEYQRARERIFADVSFSALLIFCRIQEDDACNSTQQRRQIFRLKDGRSGNSRQSSSENEPKYSEPRPWSSTDSDSSNRNLKPAMTKASSFSGISVLIRGDSSSSSKSAGRLSKTGSDSSSSVGSSTGSLSRSQPPPPPAPALTQPAHTTPLAYPAVSTSSSVSYEGGRSGPPATTANASYYLLPLEAAGIPPGSILVNPHTGQPFVNPDGSAVVYNPTVAPQASRPQQPLPPPPPPPPPPPPGPLQHQSNSHPLWPVQPSAQPVQYSAMSYPPQLLSVSPNQQYTVQDNLSAQFSHMSLVRQPSGEGRDAHTAMYPSSVVLQAPPPPPPPPQQQAGYMVPSPGQSVPAPPYSTPGPAPVNQPVMQQQGYMQQPMQQMPACYCAPGQYPLSSQQYRSVGTVQYSSPQSQPLPPPPPAQQPGYQTVMPNQQPSYQSMVQQPQSQTLVSGQHSNMGNQMQGMMVQYPSMPSYQVSMPQGSQGVPQQTYQQPIIIPNQSSHGPMPASSIQVYYSVIPPSQQNTMSSSVGFLPPPGTEQMSFPRTSSPCNSQQIPTQQCVPPPPPGGGMVMMQLTLPPTQQPRPHSPPQWKHNKYHSLDHQRSQKSTELCSLDTSQSSPQLGSPSTSPAQSPTPSHLKNVRPGLTSVPIMPQFSRSFVPGQGDARYPLLGQPLQYSPQVRPPLLHAPPMVSNHQGPVGIRHVGRGRKPPRKALSTDLSVGEAVSGCVLEVTELPVGISRAEADLLLRDLSRAGAVVSWLPERRADGDRSEPLPSDPASAYTVLATFPSRQAAQGALLCHSFALSPAPFRLTPSKRHCEQPSLERASSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZL9</accession>
    <name>A0AAR2LZL9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2D 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LZL9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000063230.1">
        <property type="protein sequence ID" value="ENSPNAP00000079791.1"/>
        <property type="gene ID" value="ENSPNAG00000014633.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153169"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000029">
        <property type="entry name" value="Ubiquitin conjugating enzyme E2 D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="18"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="18805" checksum="FF9AE3DB85459A0B" modified="2024-11-27" version="1">RCSFRIERLLLLSWEHGSHFTPFTDELTDLARDPPAQCSAGPVGDDVFHWQATIMGPNESPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDTEKYNKMAREWTEKYAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BPE0</accession>
    <name>A0A3B4BPE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PRDM11</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017562177.1">
        <property type="nucleotide sequence ID" value="XM_017706688.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BPE0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000455"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013827.2">
        <property type="protein sequence ID" value="ENSPNAP00000000455.1"/>
        <property type="gene ID" value="ENSPNAG00000007519.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432680"/>
    <dbReference type="CTD" id="56981"/>
    <dbReference type="GeneTree" id="ENSGT00940000159837"/>
    <dbReference type="OMA" id="CKNNRSR"/>
    <dbReference type="OrthoDB" id="9930943at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057456">
        <property type="entry name" value="Znf_C17orf113"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF11">
        <property type="entry name" value="PR_SET DOMAIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25431">
        <property type="entry name" value="zf-C17orf113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017194050" description="SET domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="3">
        <location>
            <begin position="110"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="360"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1154" mass="130947" checksum="7BE75B2D86D9E045" modified="2018-12-05" version="1">MKRGTQGLVLLPALSMADPATFQTEPCMETEPDCSTGVINNCSSEEPNKKLKVNHERCANVEQQNVDFWFCEECKEYFLEECPTHGPPVFVPDTPVALAVPDRAALTVPSGIEIIKEGQEVDVCCMDENIPKGALFGPYQGQLIAQDKPSGMHSWMMVNDDNTYKSIDGCDETLANWMRYIRMSSEENERNLSAFQHGEHIYFRVCRRLAVGERLRVWYSDDYMKRLQSVSQDSIDHNLDMGLNSEDLTQHQEETQGPILRSALHGRRTLSKHGSDEADSQPQAKKKKIDLIFKDVLEASLEASQSQDNPLNSTLSLPRARTQVANVFNQSSSFSHHVAESLLGQTESKVALVASGLAPVEQLESSLGERYVKMENAEEEPAMTNDGPSTSFCPNCVRLKRRIRELEAELLRFKQQEQTENLAPSEPLPSDDLRAAEAMTPIPVVLEEDDQDVDSADESIAADMMVAADDTSKLSVGPGRRIRRFKQEWLKKFWFLRYSPTLNEMWCHVCRQYTVQSSRTSAFIIGSKQFKIHTIKLHSQSNLHKKCLQLYKLRMHPEKTEEMCKNMMTLFNAAYHLAVEGRPFSDLRPLAELLKKCELKVVDQYMNESDCQILIHHISRAFKEDLAEKIRLSPFLGVIMDGQNDDLLADTVAVYVQFITAEGPPATEFLSLQRLSGGSVDGYLQAVDRAFGVLGLRFQDMLVVGLGIDGSNISSSLRANLYVTLRKTIPYVLCLPIMIHRPHMEILDAICGKELSCLDDLENNLKQLLSFYRYSPRMMAELRSTAPTLSEETEFLGDIKAVRWIIGEPNVLNALIKDYLEVVTHLKSISSQTQRADASAIALSLLQFLMDYQSVKLIYFLLDIIAILSRLAFIFQGDYLLVSQVDAKIEEAIQEIGQLVDSPGEYLQEFEENFRESFNGVDLKNLRVAESKFQSIREKICQRSQGILAQRIDQRSRSVVQACKVLDLATWPRNRDDLRMYGEEEILLIFDHLETIPSSGRELSHDRIDIRGSLVGEWRDLKADYYSMNGFKEVMSHICRYKQRFPLLNQILQVVRILPTSTACCDKGRESLHKVRRNNRSRLTLEQMNDLLTLAVNGPPIANFDGKRPLDSWFEEKSGNSYTLSAEVLSRMSNAEQKAVLHTMDVNSEFYPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BR71</accession>
    <name>A0A3B4BR71_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Muscarinic acetylcholine receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HRH1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Postsynaptic cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560236.1">
        <property type="nucleotide sequence ID" value="XM_017704747.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BR71"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002133"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010968.2">
        <property type="protein sequence ID" value="ENSPNAP00000002133.1"/>
        <property type="gene ID" value="ENSPNAG00000001104.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431530"/>
    <dbReference type="CTD" id="3269"/>
    <dbReference type="GeneTree" id="ENSGT00940000160690"/>
    <dbReference type="OMA" id="ITFMVMA"/>
    <dbReference type="OrthoDB" id="10071887at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15050">
        <property type="entry name" value="7tmA_Histamine_H1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000147">
        <property type="entry name" value="Histamine H1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000995">
        <property type="entry name" value="Musac_Ach_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF223">
        <property type="entry name" value="HISTAMINE H1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00243">
        <property type="entry name" value="MUSCARINICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0770">Synapse</keyword>
    <keyword evidence="8 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="54"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="533" mass="62040" checksum="034A51BA3525CFD2" modified="2018-12-05" version="1">METTPYSRVKKILQDHSNLSSTAYSNVSTPFHHHMNNAMLGILLGMLSLLTVIMNILVLFAVRNERTLHTVGNLYIVSLSLADLIVGATVMPLNMVYLLEDEWRLGRVVCQFWLVMDYVASTASIFSLFILCLDRYRSVRQPLKYLKYRTRGRATLMICAAWLLSMTWIIPILGWRMFAHVDRKPELESKCDTDFRFVTWFKVLTAILNFYIPSFLMLWFYSQIFIAVREHYKQWESITCPRVPTDQDGTIQNSNQLQRTFYKPSEKENLALRATFSKNDGILDQYTLEQPFSSKDTNEEIDDSSLDEKKPKNRKTSFFNISKRKRKSVRELNEISSMSQEEDIEDEASSKEPSFPLRFLHCEENDELKMFVSVNDCNVLVPNSVANICEITPTTDVHKYTTVLCSDDVAQSPTSPWGHSTSPPMDTSNTLTLKQTWQKFCVQSRQCVQNMRVHKERKAARQLGFIIGTFMVCWIPYFITFMVMALCNTCVHHDLHMFTIWLGYINSTLNPFIYPLCNENFKRVFKKIFHIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRU6</accession>
    <name>A0A3B4BRU6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger protein 367</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558209.1">
        <property type="nucleotide sequence ID" value="XM_017702720.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRU6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012395.2">
        <property type="protein sequence ID" value="ENSPNAP00000001305.2"/>
        <property type="gene ID" value="ENSPNAG00000008097.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430304"/>
    <dbReference type="CTD" id="195828"/>
    <dbReference type="GeneTree" id="ENSGT00670000098074"/>
    <dbReference type="OMA" id="NKHPHVI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000474">
        <property type="entry name" value="zinc finger protein 367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000535">
        <property type="entry name" value="Zinc finger protein 367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF26">
        <property type="entry name" value="ZINC FINGER PROTEIN 367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="106"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="278"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001305.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="355" mass="39164" checksum="E34914BA6B7076DD" modified="2024-11-27" version="2">MLFTALRLLRGGFYAARFVSKALGLFYYCIILDINPCSSPAMADSSVKQPQVIFCNDSPKRVLVSVIKTTPIKPKTADSAMPTSPGFSDFMVYPWKWGENAHNVTLSPGSGSGAASPTRNGSAAETDGSSAEHLKDGIRRGRPRADTVRELISEGENSTSRIRCNICNRVFPREKSLQAHKRTHTGERPYLCDFPECGKAFVQSGQLKTHQRLHTGEKPFVCSEKGCGSRFTHANRHCPKHPYARLKREEPTGEPGKSQGADNKAVAEWLAKYWQMREQRAPASSKGKTLSKVAMEDQEQQDPMDFLPSDEGEEEEPEEGKSSSGGGAARRRLQEQRERLHGALALIELANNLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSE2</accession>
    <name>A0A3B4BSE2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000002568"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010224.2">
        <property type="protein sequence ID" value="ENSPNAP00000002568.2"/>
        <property type="gene ID" value="ENSPNAG00000009004.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183166"/>
    <dbReference type="OrthoDB" id="2113814at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045334">
        <property type="term" value="C:clathrin-coated endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000007">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000095">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.730:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015348">
        <property type="entry name" value="Clathrin_H-chain_linker_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022365">
        <property type="entry name" value="Clathrin_H-chain_propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF6">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09268">
        <property type="entry name" value="Clathrin-link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01394">
        <property type="entry name" value="Clathrin_propel"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clathrin heavy chain linker core motif" evidence="8">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="538"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="687"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="834"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="980"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1129"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
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            <begin position="1275"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1424"/>
            <end position="1567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002568.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1683" mass="191229" checksum="8CE505D533CFF008" modified="2024-11-27" version="2">MAQILPIRFQEHLQLQTIGVNPANIGFSCLTMESDRFICIREKVGEQNQVVIIDLSDPSNPIRRPITADSAIMNPASKVIALKGAARTLQIFNMEMKSKVKAHTMTDEVMFWKWISINTIALVTDTAVYHWSMEGDSQPIKIFDRHASLAGCQIINYRTDEPQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFVQYKLEGNPNPSTLFCFAVRSQAGGKLHIIEVGQPAPGNQPFAKKAVDVFFPPEAQTDFPVAMQIGAKHGVIYLITKYGYIHMYDLESGVCIYMNRISAETIFVTAAHESTSGIIGVNKKGQVLSVCVEEENIVNYTTNVLQNPELGLRMAVRSDLPGAEELFTRKFNTLFTQGNYAEAAKVAASAPKGVLRTSETIRRFQSAPAQGGQASPLLQYFGVLLDRGQLNRLESVELCRPVLQQGRMQLLEKWLKGEKLECSEELGDLVKAADPTLALSVYLRANIPAKVIQCFAETGQYQKIVLYAKKMGYSPDWIMLLRSVMRSGPDQGLQFAQMLVQDEEPLANINQVVDVFMEGNLVQQCTSFLLDVLKNNKPDEGHLQTRLLEMNLIHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAIVHTHLLNPEWLVNFFGSLSVGDSMECLRAMLAANLRQNLQLTVQVASKYHEQLGTATLVELFESFKSYEGLFYFLGSIVNFSQEPEVHFKYIQAACKTGQIKEVERICRESSCYDPESVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVVGGLLDVDCSEDVIKSLIIAVRGDFSTDLLVEEVEKRNRLKLLSPWLEARAQEGSEEAATHNALAKIYIDSNNGPERFLRENPYYDSATVGRYCEKRDPHLAFVAYERGQCDMELIKVCNENSLFKSEARYLVRRKDPDLWAVVLEENNPYRRALIDQVVQTALSETQDPEEVSVTVKAFMTADLPTELIELLEKIVLENSIFCEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELYEEAFSIFKKFDVNASAIQVLIEHIGNLDRAYEFAERCGEGPVWSQLARAQLQKNLIKEAIDSYIRADEPSAYMEVVDAASRTDNWEDLVKFLQMARKKARESYVETELLFALAKTNRLSELEEFINGPNNAHIQQVGDRCYEEGMFEAARLLFNNVSNFARLASTLVHLGEYQAAVDSARKANSTRTWKEVCFACVDGEEFRLAQICGLHIVIHADELEELISYYQERGYFEELIMLLEAALGLERAHMGMFTELAILYSKYKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMIAHPTDAWRDASFKEIISKVANVELYYKSLQFYLEYKPMLLNDLLTVLAPRLDHTRAVSFFTKVNQLTLVKPYLRSVQSHNNRAVNEALNSLLTEEEDFQGLRASIDAYDNFDSISLAQKLEQHELIEFRRIAAYLYKINQRWAQSIQLCKKDNLYKDAMQYAAESKESEHAEALLRWFLEEGRRECFAACLFSSYDLLAPDVVLELAWRHGLIDFAMPYFTQVMREYLTKVDELAGQVKVDKLEEAESKRSTEEGSEPTSIVYGQQLMLTAGQTPVAPQAGYPGYGYTAPGYAPPAYGFNLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSL4</accession>
    <name>A0A3B4BSL4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ZNF646</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017551430.1">
        <property type="nucleotide sequence ID" value="XM_017695941.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017551431.1">
        <property type="nucleotide sequence ID" value="XM_017695942.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017551432.1">
        <property type="nucleotide sequence ID" value="XM_017695943.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017551433.1">
        <property type="nucleotide sequence ID" value="XM_017695944.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000001469"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012115.2">
        <property type="protein sequence ID" value="ENSPNAP00000001469.2"/>
        <property type="gene ID" value="ENSPNAG00000000750.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426464"/>
    <dbReference type="CTD" id="9726"/>
    <dbReference type="GeneTree" id="ENSGT00940000164729"/>
    <dbReference type="OMA" id="MECHQER"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000414">
        <property type="entry name" value="Zinc finger protein 398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000729">
        <property type="entry name" value="Zinc finger protein 646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001345">
        <property type="entry name" value="zinc finger protein 646"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002243">
        <property type="entry name" value="zinc finger protein 646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002353">
        <property type="entry name" value="Zinc finger protein 646"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF127">
        <property type="entry name" value="BLOODY FINGERS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="27"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="677"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="736"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="845"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="872"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="900"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="993"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1020"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1048"/>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1139"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1166"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1194"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1223"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1262"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1313"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1341"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1431"/>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1458"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1486"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="1514"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="333"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="483"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="696"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="948"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="915"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="704"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="957"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="968"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001469.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1563" mass="175936" checksum="36DCA235CFAA8892" modified="2024-11-27" version="2">MDIQEPSRTKGFLCKNCGIVCSNMSSFMEHTEACQQSESDRKFKCDECGRGYRHAGSLANHKKTHEVGTFQCHICARKLSNALALKSHLRIHTSRKKYSCTECGKAFRLATQLATHQKVHRNQESRGRTKSNDLTHNQTESICEDDQLQQVGEQDFSTASDLSPDIGLDVAPENSFSNGLMSELIPSFAPESDAFANGDDEDRPYKCDMCEKTYRHHGSLINHKKSHQMGIFDCTVCYKQFNNLAALNSHQRIHYKIKGRPNSVTQVTPEAQFQNPKSEQVSNVLQNGATNVYFCHLCQVAFATDDEFQNHVMLHNSSSVSFELPTNIPEDHSFSYNHSITQSPEPNLYPSSSDTPPLPPLLDKADDYDQNQGPMENGHIFVPHPIGDNSTVVDTQGEPLPLPSDNLNPELPEQATDDGKQDQSVSSDRRFKCHICGKSYRHAGSLINHKRSHQTGVFQCSICRKNYPHLAALRSHLRIHKGRPSSITTTPEGDWLSSEPLTHENQHGGFPSHEGDVDGILGLPQDLGDSVGMDSDGELDGTEFQEQFNSPFSEDGQMHLPQGEALMERHMCADCGNTFSDIAGIKSHVCPLLNQQYQSMSNGLHGHLDYKTENHQQPLGDTGECISFDGQNGSTSGTYFAGQNFHDTMHGQIVSDHEDKGHCEDDGDEEEDDGEMYQCSVCGNHYTSMRALRSHLRGHTQTHGTPSTSGPSSMSSLEAEKDDEPSEIQQGDCSLIICSTCGESFLKKQDLLAHQLLHSNTLVGQQEQYVTENQNQVKPKEETDSIICGKCGMVSASVHQLESHNCTGQRTEELEQHDEKPDIGSVRGQEVGYFQESPSSGDRQYRCEQCGRSYRHAGSLLNHKKSHKTGVFRCFVCQKRFYNLLALKNHQRTHFDVKKHSCTECGKAFKIHKQLLNHQRVHQENKAKIEELNKQIQTLMQMSGNVSVSGVQPLGINRKRSSRRRRQKDTPHSDQSSPEKDCQSGDSSDPRPFVCDQCGRSYRHAGSLVNHKNSHKTGEYYCAVCNNTYSNQLAMKNHLRIHFSVKKHCCQDCGKAFRGKKQLLNHTCSHNRKNAAAVVRGKGRGRRRAKALKCKDCSLVFHTANQLASHICSNQPCSNSATEQSSNDSVPVVKEERPFKCNICGRSYRHAGSLLNHKNTHKTGHFTCSFCAKPFSNPMALRNHTRIHTQKKKYVCPTCGKAFRLSSILYNHQKVHARGVTRYSCQTCGKSFQGKSGLKRHRCYKSVTSTSALNQDAGDKCYTCDQCGRSYRHAGSLLNHKKTHSADLLHCTLCLKTFTDPLELQSHSQMARHCCPDCGKTFCEFAHLQSHMEVHSKGLPYYCSLCQQNFPNIASFQQHQEVHHCVQGQSHHQQGMQMSQDLDWDSGLDQQMGLQDLPKMDPAFSRIQEDFSEQQVPQESSEEFAREEKSHVCEHCGRTYRHAGSLLNHKNSHKTGSFICSVCQKKFNNLMALKNHRRIHTEPKRYQCLECGKAFRVSTQLICHRRIHTKEKPFSCLLCEKRFSSKSNLRHHQKMHQNAQQSFESSFSMDPNAFMGLGVDPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BT77</accession>
    <name>A0A3B4BT77_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF234</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BT77"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002848"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009741.2">
        <property type="protein sequence ID" value="ENSPNAP00000002848.2"/>
        <property type="gene ID" value="ENSPNAG00000034873.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286918"/>
    <dbReference type="OMA" id="CARHERT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001968">
        <property type="entry name" value="chorion transcription factor Cf2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003287">
        <property type="entry name" value="Zgc:113343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000355">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000848">
        <property type="entry name" value="Zinc finger protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002604">
        <property type="entry name" value="Zinc finger protein 715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="624"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="652"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002848.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="714" mass="82952" checksum="B79E42262074BE3D" modified="2024-11-27" version="2">MFRYGTLQLHQRTHTGEKPYHCSECGKSFRQQSNLLVHQRIHTGEKPYQCSECGKNFNRLDVLQHHQRVHTGEKPFKCSVCGQSFRQQNNLQVHQRIHTGEKPYCCPYCERSFSRHSNLKEHERTHSGEKPYQCSECGKSFRQHTNLQVHQRIHTGEKPHSCLECGKTFNRNLQVHQRIHTGEKPFQCTECEKSFIDRGTLTKHQRIHTGERPFDCSLCGKCFRDNDTLKKHHITHTGEKSYYCSCCGRSFSRYSNLQLHQRTHTGEKPYVCSDCGKHKRTHTGEKPYQCPDCGKSFYRGSHLQTHQRVHTGEKPYHCSECGRSFSHLRSLQRHECIQTEHKSHQCLDCGKSFTHLSNLQTHQRIHTGERPYQCSECGKTFNDHGNLQAHQRTHMEEKPYCCSECGRGFTVYGLLQRHQQIHRGEKEHHCPECGKSFSCQRSVQRHQLVHTGEKPYQCSECGKSFNNQNNLQVHQRIHTGEKPYECLECGKTFNNQNNLQVHQRIHTGEKPYVCFQCGKSFGDRGAFKAHQRTHSLEKPYHCSECGKGFKEKGTLKRHQRIHTGEKPFCCSDCGKSFRDRSTLNTHQRTHTGEKPYQCSQCGKSFTVHSSLQLHQRIHTGEKPYHCSECEKSFTLQSHLQRHQQIHTGEKPYHCSVCRKNFRHSSTLKIHKCAPMRDGDNSVTVVLEPDSSDEAEDPALISIPTKVLKEEHHLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BU56</accession>
    <name>A0A3B4BU56_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridylate-specific endoribonuclease</fullName>
            <ecNumber evidence="11">4.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ribonucleotidyl-uridine-RNA = a 5'-end dephospho-uridine-RNA + a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA</text>
            <dbReference type="Rhea" id="RHEA:67792"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17354"/>
            <dbReference type="Rhea" id="RHEA-COMP:17356"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173117"/>
            <dbReference type="ChEBI" id="CHEBI:173224"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ENDOU family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BU56"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011058.2">
        <property type="protein sequence ID" value="ENSPNAP00000002069.1"/>
        <property type="gene ID" value="ENSPNAG00000008556.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063825"/>
    <dbReference type="OMA" id="YRHGRHI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21159">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039787">
        <property type="entry name" value="ENDOU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF33">
        <property type="entry name" value="URIDYLATE-SPECIFIC ENDORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="EndoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
        <property type="entry name" value="ENDOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="EndoU" evidence="12">
        <location>
            <begin position="7"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="285" mass="32495" checksum="4C22201DD03E114C" modified="2018-12-05" version="1">MAKGPAVDQELSAVLNELWKLDVNRLKPGRDYTINLQGKADFVAQGSNNARDKANTPLFSYVNEDKLKSIATFACFINLLDNYEMSTGVAEKVTSEELEENDLFLDAILKTEVMKRTHKYLVSKGQAKSDIAQFKKQLFDIWFKLYNRDRSGGEDSCGFEHVFVGETKYNKEIMGLHNWVQFYLQEKLNYVDYKGYKGGDKDKPTADDHVVNLQFSWKGLVKPLGSSFIGVSPEFEVALFTIAFLMNNERVTKTVVKVDKYVLEIVVCRYGRAIGTSYPKMLQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BV83</accession>
    <name>A0A3B4BV83_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alkylglycerol monooxygenase</fullName>
            <ecNumber evidence="12">1.14.16.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Transmembrane protein 195</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">AGMO</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Glyceryl-ether monooxygenase that cleaves the O-alkyl bond of ether lipids. Ether lipids are essential components of brain membranes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-O-(1,2-saturated-alkyl)-sn-glycerol + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2 = a 1-(1-hydroxyalkyl)-sn-glycerol + (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:36255"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="ChEBI" id="CHEBI:73418"/>
            <dbReference type="ChEBI" id="CHEBI:83957"/>
            <dbReference type="EC" id="1.14.16.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sterol desaturase family. TMEM195 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.5" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BV83"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002409"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010484.2">
        <property type="protein sequence ID" value="ENSPNAP00000002409.2"/>
        <property type="gene ID" value="ENSPNAG00000008797.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033807"/>
    <dbReference type="OMA" id="FMPTGWR"/>
    <dbReference type="OrthoDB" id="6354873at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050479">
        <property type="term" value="F:glyceryl-ether monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006643">
        <property type="term" value="P:membrane lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056853">
        <property type="entry name" value="AGMP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051689">
        <property type="entry name" value="Sterol_desaturase/TMEM195"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21624:SF1">
        <property type="entry name" value="ALKYLGLYCEROL MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21624">
        <property type="entry name" value="STEROL DESATURASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24858">
        <property type="entry name" value="AGMP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="17">
        <location>
            <begin position="117"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Alkylglycerol monooxygenase C-terminal" evidence="18">
        <location>
            <begin position="334"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002409.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="450" mass="51731" checksum="EE38C7EC117703E9" modified="2024-11-27" version="2">MAQLESGDVTFSQGTRMLFYLMTPNETSFSDVDSVPRYVQKATPFFIGLVLLEVFVSWLKTGELLVKINDGTTSLSAGMISRLPHVFVRSLEVSSYIYVWNNYHILELPWDSAWTWWLAFLGVDFGYYWVHRCCHELNILWAAHQVHHSSEYYNLTTALRQSVTQQCASWVFYLPMALVVPPSVFAVHIQFNLLYQFWIHTEIIRDLGPLEWILNTPSHHRVHHGRNLYCIDRNYGGTLIIWDRLFGTFTPEGDKVVYGLTRPINTFDILSVQFHYYSYLWEQFLKADGIGHKLSIIFKGPSWAPGKPRLGDHAEVPIVTGEEVPHNPYWSLRLQVYVHIQFLLVLGVYNDLFATQYPLSQMTILLITGYILLTLTSLGYIIEQRPNAAVWEALRCLIMMALYRFGYVGPVVSQLALRPIMADRISLCVCVCIYVVCELAPGSTTQHSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVP0</accession>
    <name>A0A3B4BVP0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Regulator of G protein signaling 14a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017559329.1">
        <property type="nucleotide sequence ID" value="XM_017703840.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BVP0"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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            <dbReference type="PROSITE" id="PS50898"/>
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        </source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="64141" checksum="A5FED6EC54D11C81" modified="2018-12-05" version="1">MVAVRGLAKALASRWALRGSFQKKVSTRLTPKMLPTQHKMQAVSDGELNMSRLSGTGSSPSLNGSSSGNAGPPGADGPASRVVSWAVCFERLLEDPVGVRYFTAFLKSEVSAENILFWQACEKFRKIPSTNLDELKKEARTIYDLYLSDTSFHAVNIDDTARTEESDLEKPTPDMFSKAQQQIFKLMKFDSYARFVRSPLYQNCMLASVEGRPLPDLQPQNKGSRECVVPAGDRKSMEVKMAFSDSKAGKKKMMIEKRGSWGADVSYRRVTVSRQESQISVKSSSSVELGSLPGRSENGCSSPIGLDPDSGSGGMRADKYCCVYLPDGTASLAPARPGLSLRDMLSSLCEKRGFPLKDVVIYLHGKDKQPLSLDQDSSVLRDQQVHLELRVTFTLEVVFAGKASGIVAKSSKTLGEALSSVLQKHNLRLQDVVVSVSGSKEPLNMNTSVFGLANKTLSLDRAKSAGEVKGAPAAETGRTSLRPKRMHDMDGLVEMLNRAQCSRADDQRGLLTKEQLELPQFLQLPVEQKDEQSPKGPESPSNHTHSAGSSEEQRKRNINGVNKDPSKEKTVKDCKKDKIGSNNGTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXD1</accession>
    <name>A0A3B4BXD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ZNF385D</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037389666.1">
        <property type="nucleotide sequence ID" value="XM_037533769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BXD1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003134"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009250.2">
        <property type="protein sequence ID" value="ENSPNAP00000003134.2"/>
        <property type="gene ID" value="ENSPNAG00000009509.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439301"/>
    <dbReference type="GeneTree" id="ENSGT00940000160242"/>
    <dbReference type="OMA" id="RNIMYFX"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 385B isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051845">
        <property type="entry name" value="Znf385"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 385D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="277"/>
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            <end position="333"/>
        </location>
    </feature>
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        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
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            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003134.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="409" mass="43449" checksum="2D135DE11B72ABD3" modified="2024-11-27" version="2">MESSSMFLGNVCQSGLLPLVRAPLPLQSSLRLKQILPFTLDASPLSLVPGFSSMDPVQKAVLHHTFGVAASAKKKHASCNVCQLRFNSQSQALAHYKGTKHAKRLKSLDAPKCKQKSLVVARENASKEPSKSLSLPAPLQNGTDWKEGSPVPPSPVQSGSSSGPAEAQSTSAAPAAGGEESPCQTPGAPQSTEQEGEPEPETEKGETEEEKARRLLYCSLCKVAVNSPSQLEAHNSGTKHKTMLEARNGNGSIKSFPRPGVKSKLTTPTGSTTGLQNKTFHCETCDVHVNSETQLKQHISSRRHKDRAAGKPAKPKYSPYTKTQRGQTKQQTQVKLPLGKDLCQPLTATIVPSHLAAVAAAAAAVGSAFPLRANHNPSVFQTQSLPAALLRPAPGPIRTSHAPVLFAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C132</accession>
    <name>A0A3B4C132_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C132"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004630"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006697.2">
        <property type="protein sequence ID" value="ENSPNAP00000004630.2"/>
        <property type="gene ID" value="ENSPNAG00000011058.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000277137"/>
    <dbReference type="OMA" id="SPLGQCG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004630.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="216" mass="24098" checksum="1298A6B742A1C09A" modified="2024-11-27" version="2">MGNLISRPSCLGQKSKQVRSNEDFLKECYQRQREWPLPEPPREERKNVDNVDHVDQVPPQVPTPDKNRSSPAPTVTTTSTLDNAWRSTPSPAKTPYNGSLPRRANVPEYRRSPLGQCGTAEGRATLQRRDSGSPWSWKTLSSREVTEVTEVTETIVTEIVEVTEYPAGQKGGDPIVTRTVRVLTGAAEELAELQNDGQSSTEQDSSLVVWKVPFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C2S9</accession>
    <name>A0A3B4C2S9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">METTL5</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547151.1">
        <property type="nucleotide sequence ID" value="XM_017691662.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_037539664.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A3B4C2S9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006212"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003966.2">
        <property type="protein sequence ID" value="ENSPNAP00000006212.1"/>
        <property type="gene ID" value="ENSPNAG00000002178.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423964"/>
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    <dbReference type="GeneTree" id="ENSGT00390000000227"/>
    <dbReference type="OMA" id="DVVYSIH"/>
    <dbReference type="OrthoDB" id="419617at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008988">
        <property type="term" value="F:rRNA (adenine-N6-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="3">
        <location>
            <begin position="43"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00041374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="207" mass="23331" checksum="B100C1284DF7EB21" modified="2018-12-05" version="1">MKLKELESCLQQVDGFEEPKILLEQYPTSPHIAGCMLYTIHNTFDDIQNKLVADLGCGCGVLSIGAAVLDAGLCVGFDIDSDALDIFKRNVEEFELPNIDMVQSDICSVGSSYAKKFDTVIMNPPFGTKHNQGIDMQFLKTALSMARKAVYSLHKTSTREHIQKKANDWKVKMEVIAELRYDLPASYKFHKKKSVDIQVDFIRFTPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3C0</accession>
    <name>A0A3B4C3C0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017554328.1">
        <property type="nucleotide sequence ID" value="XM_017698839.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017554329.1">
        <property type="nucleotide sequence ID" value="XM_017698840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3C0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005515"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005160.2">
        <property type="protein sequence ID" value="ENSPNAP00000005515.1"/>
        <property type="gene ID" value="ENSPNAG00000006535.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428120"/>
    <dbReference type="CTD" id="792588"/>
    <dbReference type="GeneTree" id="ENSGT00940000160309"/>
    <dbReference type="OMA" id="QHHSAHT"/>
    <dbReference type="OrthoDB" id="8895262at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000250">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001677">
        <property type="entry name" value="Zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001049">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001217">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000184">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000788">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF95">
        <property type="entry name" value="ZINC FINGER PROTEIN 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="462"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="518"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="546"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="574"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="184"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="610" mass="68623" checksum="F20BE50FD716911C" modified="2018-12-05" version="1">MTEAWQQHAVAPPPVVHTIPPGAENALGCAVYGIVLQPDAALQQQQSQQQQQQQQQQQQHHSQQHSQQQHPAQAQQPSLPVGSEGGHKCGACGHDISHLANPHEHQCMVSQDRSFQCTQCLKIFHQATDLLEHQCVQVEQKPFVCGVCKMGFSLLTSLAQHHNAHNSSNPMKCSICEKTYRPGSSGNATPTSSSGNQGQPSNDGASASSSAAVGTSGQPTFESSAPYRPYKCSVCSKGFRHLSELTRHERVHTGEKPFKCETCDKSFSQSSHLVHHQSTHSSERPFKCAVCEKSFKHRSHLVRHMYAHSGEHLFKCNMCELHFKESSELLHHQCQPQGARPFRCATCGKGFKRPSDLRQHERTHSEERPYHCEDCQMSFKQQYALVRHRRTHTASADRPFKCNLCDKGFLQPSHLLYHQHVHGMDNLFKCASCGKGFSQSGELLRHKCGESSSTPTNPDKPYKCDVCGKAYKKATTLQRHQTTHCNEKPLKCSLCDRRFLSSSEFVQHRCDPSREKPLKCPDCEKRFKYSSEMNRHRRVHTGEKPYKCASCDKGFKQREHLAKHQSVHSRDAQFKCVWCGERFADLGALQEHTVQHTAEGGGYPVNSLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3J2</accession>
    <name>A0A3B4C3J2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3J2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005164"/>
    <dbReference type="Ensembl" id="ENSPNAT00000005768.2">
        <property type="protein sequence ID" value="ENSPNAP00000005164.2"/>
        <property type="gene ID" value="ENSPNAG00000033315.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272426"/>
    <dbReference type="OMA" id="NKKFCID"/>
    <dbReference type="OrthoDB" id="8934837at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF183">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043388728" description="Chemokine interleukin-8-like domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="6">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005164.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="99" mass="10809" checksum="6C37D113EEC4FAF1" modified="2024-11-27" version="2">MRTLSALLVLLLICSLQLTSSAPIATEQHSCCPYLTPMKIPLAQVVSYTWTSSDCHLKAIVFLTKKGKQICVDPDTVWVSSHVAAVDKLPTTLSLKKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C4W2</accession>
    <name>A0A3B4C4W2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transmembrane and coiled-coil domain family 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEX28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568023.1">
        <property type="nucleotide sequence ID" value="XM_017712534.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C4W2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004145.2">
        <property type="protein sequence ID" value="ENSPNAP00000006105.1"/>
        <property type="gene ID" value="ENSPNAG00000012169.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436190"/>
    <dbReference type="CTD" id="563581"/>
    <dbReference type="GeneTree" id="ENSGT00940000155189"/>
    <dbReference type="OMA" id="IMEKWIA"/>
    <dbReference type="OrthoDB" id="10072335at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019394">
        <property type="entry name" value="TEX28/TMCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17613">
        <property type="entry name" value="CEREBRAL PROTEIN-11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17613:SF11">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10267">
        <property type="entry name" value="Tmemb_cc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="392"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="503"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="658" mass="71446" checksum="87E491133750FB18" modified="2018-12-05" version="1">MDQGGSEQPGAEEPDSGGRAEPEVGRRASEPEHGLSKITHNALENMGALGHGLKQLFQPQRRRSSVSPHDTAASSIGPASEAMEAGPELGEASTPSATTSDSDAPTSAPPAALSRVLQQIRGAPPMMKRGTSLQSRRSKAGGGGEAPQKGSPQIHRRSTQEALLQAGRPRSSSTTDTPSSPALADMLLTSGYHSTEESDRVDRLEVSAPAVSPNALSISSDGGPYGVDSVDGTPDPQRTKQAIAQLQQKILKLTEQIKIEQTARDDNVAEYLKLANNADKQQSARIKQVFEKKNQKSAQTIQQLQRKLEHYHRKLREVEHNGIPRQPKDVLRDMHQGLKDVGAKVTGGLSSFSQATHSAAGAVVSKPREFASLIRNKFGSADNISALKDSLDETQGDETMSGAGPSGARALAPGQLQSSPKYGSEDDCSSATSGSAGANSTTGAPGGPPSSKGNTLELGQSSGFDALFHEIQELRENQSRLDESFDSLKSHYQRDYTLIMQALQEERYRCERLEEQLNDLTELHQNEILNLKQELASMEEKIAYQSYERARDIQEALEACQTRISKMELQQQQQQVVQLEGLENATARTLLGKLINVLLAVMAVLLVFVSTVANCVVPLMKTRSRTLSTLLLVILLAFLWRNWEVLSLYLDRFLLHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7H6</accession>
    <name>A0A3B4C7H6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gastric inhibitory polypeptide</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glucose-dependent insulinotropic polypeptide</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GIP</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glucagon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C7H6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007618"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001537.2">
        <property type="protein sequence ID" value="ENSPNAP00000007618.1"/>
        <property type="gene ID" value="ENSPNAG00000013374.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005121"/>
    <dbReference type="OMA" id="ICLGSAW"/>
    <dbReference type="OrthoDB" id="8874823at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031769">
        <property type="term" value="F:glucagon receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042304">
        <property type="term" value="P:regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050796">
        <property type="term" value="P:regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039078">
        <property type="entry name" value="GIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000532">
        <property type="entry name" value="Glucagon_GIP_secretin_VIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15211:SF0">
        <property type="entry name" value="GASTRIC INHIBITORY POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15211">
        <property type="entry name" value="GLUCOSE-DEPENDENT INSULINOTROPIC POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00123">
        <property type="entry name" value="Hormone_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017391368" description="Gastric inhibitory polypeptide" evidence="8">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Glucagon / GIP / secretin / VIP family" evidence="9">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="110" mass="12403" checksum="1F6356A8C19B6BAF" modified="2018-12-05" version="1">MKMKVELIALVFLCMGVVLAVGARAVDNSPADDGQRLDRRYAESTIASDISKIMDSLVQKNFVNFLLNQREKKSMPTMTSEDPDIHTFSDLLKKDFTMLIHSKGDRSKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7U9</accession>
    <name>A0A3B4C7U9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoethanolamine/phosphocholine phosphatase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PHOSPHO1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541831.1">
        <property type="nucleotide sequence ID" value="XM_017686342.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C7U9"/>
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    <dbReference type="Ensembl" id="ENSPNAT00000002613.2">
        <property type="protein sequence ID" value="ENSPNAP00000006991.1"/>
        <property type="gene ID" value="ENSPNAG00000012917.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413699"/>
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    <dbReference type="GeneTree" id="ENSGT00390000007741"/>
    <dbReference type="OMA" id="FHSHECQ"/>
    <dbReference type="OrthoDB" id="10267182at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006384">
        <property type="entry name" value="HAD_hydro_PyrdxlP_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
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        <property type="entry name" value="Pase_PHOSPHO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01489">
        <property type="entry name" value="DKMTPPase-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889">
        <property type="entry name" value="PHOSPHATASE, ORPHAN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE_PHOSPHOCHOLINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06888">
        <property type="entry name" value="Put_Phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031051">
        <property type="entry name" value="PyrdxlP_Pase_PHOSPHO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="32346" checksum="7221A45BEE7607B4" modified="2018-12-05" version="1">MRDSVFNCCFAPPHPPPGRRDESHFQGNRALAPPPNDRRFLIFFDFDETLVDECSDDAVVSAAPGGSLPSWLKDTYRPGRYNEYMQRVLAYLAEQGVTPGTIISTIERLPPCPGIPALLRFLMSRPPRDFEVVCVSDANTVFIEAWLQNLGFRQLFLRIFANPAHFDDNGQLQLRPFHSHSCLRCPMNMCKAEIVRQYTAQRVRERGGRRYQKVLYVGDGANDFCPSLTLSPGDTAFPRRDFPMHRLIQEMGEAKPGEYKANVVPWSSGEDIINRLRKIVEEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C7Y5</accession>
    <name>A0A3B4C7Y5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tantalus-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PRR14</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017570954.1">
        <property type="nucleotide sequence ID" value="XM_017715465.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000008017"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000857.2">
        <property type="protein sequence ID" value="ENSPNAP00000008017.1"/>
        <property type="gene ID" value="ENSPNAG00000013679.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437995"/>
    <dbReference type="CTD" id="78994"/>
    <dbReference type="GeneTree" id="ENSGT00520000055626"/>
    <dbReference type="OMA" id="DSWMALE"/>
    <dbReference type="OrthoDB" id="6163216at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026320">
        <property type="entry name" value="PRR14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028149">
        <property type="entry name" value="Tantalus-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14522">
        <property type="entry name" value="EMO2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14522:SF2">
        <property type="entry name" value="PROLINE-RICH PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15386">
        <property type="entry name" value="Tantalus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tantalus-like" evidence="3">
        <location>
            <begin position="1144"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="558"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="771"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="842"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="915"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1108"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1216"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="777"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="844"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="863"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="923"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="940"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1022"/>
            <end position="1043"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1287" mass="142022" checksum="C638EBB0050FB8F8" modified="2018-12-05" version="1">MLTSSQERVPRSVTPMEEDVLCAVHHDEPRPQTVHRSSVLNSCDEVRRSSSRRRSGRLLKGKGQTEERIKPENSVRTPELQQKNTSLIMKERGSWKNHEDNEEIMDMWPADKTVRSCKRKLELPSITSESHVLKNSSTVQEHSRSSLDMDPKCPESPPPAKRWVIGPLLQSFKSKMASFTEIVMSPVRLFKPTDSSLSNSLTSHCEQLTGSNKESSSISKEQQPDVERDVTKDLFSKEETVCPPSKISIVVERLNFNTNLSNESDSDENKALVSPSTSDIQRNGVQCLLQSIQEGHGSLNSSARDLSCHVEQPMGPSNEYQNKVYNATSVSEQSGSKSSQEKSRFSDPRLLTDTPDTIDLKLAHVEVPCPNTSCSLSLTKNTNRKGLMDSEESILRMPIRTSPRKMSKLPLDSNRLTPLRSAKKVSVTLSEQNILVGATKVSEYTHNNKARIQPFSKDDGVPPLGCERKNRKTVPKIELVRCLEKDKLVKKVKSCSGRTKIENKANFEQEFPASILARVPVNETSIQIGKRRKVQVSGNLTLSSRGGVEMMSFASEMSGSCESPERDKIGQERVSRSRSRRDGKCRTPATLGIENDTIGSLSSSDASKSALVDSCLQGNGTSGMGASDCNDSNPVEARPSLRSRGMVTVGGSVKSVKSWNARQRKTACHFESGVSLGSSTEQPGEPWAKNGHRDLEEASNATDQEQAKFAQKNNVCQAKNKLDASAVASVLSSSKKPLVHVKRLESREVKDIDAVEEPRVSMSLTLQVGTKTDEQKGSVTLSPVQTGSTSQWQAGRPIENCRTTRGMGKWGTPAKEINTSKTALVHFKDQSFIDKELGQLGKAKEKKKSKVGRPRRKCISLLRKKDARDDEEQTRNQTEEAIPVVSSSGSGSSRLLRSFSCPDIPSLLHGDNVPLAPLHDQIPPSPPKKFCPAPPDAHHPHSPSKRARRHTVCSVEIEREIAPRCLRKEVHPTGWSSASNHVYPHSHSSSLTALASCFLSSPLAFLSKKSSQGRSDDDVDLASTSPRSFVKSPSPSFLKSPTSPTLTANPAFFASVPPTPEQSSASVSSPCSVFEPVPVEADVVQREREEDSQSSFSLKLSSRSISEERALSDSEIKTDDKREERRKVSSIRIRKTLPKPQYNLTPMGLPKAIRIKKKVFSVEEIYTNKNFSKPPEGRFETIFEVPQSRQDSSQSLFGQKRMKRFIEFPELGVPRKPRKPRKPLVGGAGVGGAQRKAAGNSGIGRTRRGVWASSRDEDALNLQDLDSLLCSKLNELDSWMALEQLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8K5</accession>
    <name>A0A3B4C8K5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8K5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002165.2">
        <property type="protein sequence ID" value="ENSPNAP00000007241.2"/>
        <property type="gene ID" value="ENSPNAG00000013107.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007241.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="58" mass="6359" checksum="15FD4F09B80CD850" modified="2024-11-27" version="2">NLKLALQEATLPTSLEHHMYWSGLPGLHPACTPSPCTDNGHPGRHSTAREREGCRQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9U2</accession>
    <name>A0A3B4C9U2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CHST12</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9U2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008707"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014841.2">
        <property type="protein sequence ID" value="ENSPNAP00000008707.2"/>
        <property type="gene ID" value="ENSPNAG00000030191.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156614"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF4">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008707.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="404" mass="48006" checksum="09474BF3D556950F" modified="2024-11-27" version="2">MKKLRYFYVLSTTAGVLLMFFTNRYWTNRSEAEEFIRPSPNTTLFNPEHGYSEPKTLSSSYFWSNLHHYYNLVMKEFATSDEETDEENDLFEDPELENRRREWRIHSSPIPLELKHRQYARKKLIHDLCDSNGTLGFNGKWQTIDDIPSWELANLLVDDRHGIIYCYVPKVACTEWKRIMIVLSESLKVNGVPYRDPSDIPVELIHGNSNQYLNRYNRTVMKQKLQQYKKFMFIREPFIRLISAYRDKFQKENQPFYKQYGIPILKRFGKIPNPPASVKEALAAGIAPSFSNFIQYLLSLPADSYELLDEHWRQTAHLCHPCQIHYDFIGKMETMEEDAAHLLRMLRVDNIVQFPKIKSSTTDENWIKTWFPTITADSRRKLFKLFETDYKLFGYQVPEYLLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CBZ5</accession>
    <name>A0A3B4CBZ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fructose-1,6-bisphosphatase isozyme 2</fullName>
            <ecNumber evidence="8">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">D-fructose-1,6-bisphosphate 1-phosphohydrolase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Muscle FBPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">FBP2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homotetramer. Interacts with ALDOA; the interaction blocks inhibition by physiological concentrations of AMP and reduces inhibition by Ca(2+). Interacts with alpha-actinin and F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Myofibril</location>
            <location evidence="4">Sarcomere</location>
            <location evidence="4">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 24">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017551013.1">
        <property type="nucleotide sequence ID" value="XM_017695524.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CBZ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008820"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014993.2">
        <property type="protein sequence ID" value="ENSPNAP00000008820.1"/>
        <property type="gene ID" value="ENSPNAG00000014425.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426202"/>
    <dbReference type="CTD" id="8789"/>
    <dbReference type="GeneTree" id="ENSGT00390000015513"/>
    <dbReference type="OMA" id="NSRFWEP"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="PRK09293.1-1"/>
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        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE ISOZYME 2"/>
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        <property type="entry name" value="F16BPHPHTASE"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="18 24" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="16" id="KW-0965">Cell junction</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="13 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="25">
        <location>
            <begin position="13"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="26">
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            <begin position="204"/>
            <end position="333"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00042757"/>
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            <dbReference type="ARBA" id="ARBA00043165"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="338" mass="36851" checksum="1CD7BA1BAF46D699" modified="2018-12-05" version="1">MTDQSAFDTDVWTLTRFVMETGRQVKGATGELTQLINAMLTAIKAISSAVRKAGLAHLQGIAGHINVTGDEVKKLDVLSNDLVINMLQASYSTCVLVTEENKEAVITPAEKRGKYIVCFDPLDGSSNIDCLAPIGTIFAIYKRSSEGEPTEKDALQPGNQIVCAGYALYGSATVVAMSTGAGLNFFMLDPAIGEFILTDRNVKIKKKGKIYSLNEGYAKYFDSAVTEYIKHKKFPEDGSAPYGARYVGSMVSDVHRTIAYGGIFLYPANEKSPKGKLRLLYECNPIAFLVEQAGGLATTGTQRILDVQPEALHQRVPFVVGSPDDVEEYMAFVKKYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDI5</accession>
    <name>A0A3B4CDI5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017556230.1">
        <property type="nucleotide sequence ID" value="XM_017700741.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000008936"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015131.2">
        <property type="protein sequence ID" value="ENSPNAP00000008936.1"/>
        <property type="gene ID" value="ENSPNAG00000003149.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429182"/>
    <dbReference type="CTD" id="795766"/>
    <dbReference type="GeneTree" id="ENSGT00940000156497"/>
    <dbReference type="OMA" id="NQEDHES"/>
    <dbReference type="OrthoDB" id="5836549at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF9">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="536"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="833"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="908"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1039"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="870"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="924"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="962"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="993"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1048"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1239" mass="137507" checksum="778A5F0EDDD67704" modified="2018-12-05" version="1">MAIPTMLHSSSSILEASAMANLTEDTSSSGTDMGHDPMIQTETATLLLQETGMEEAGITAVAAQTEGERWQEFNPSLVEHNLFQSQFRSSSSEAVALLMHPRDTHSVTPSLRIHSKEPREGTVEGRDRHKEANGLGQQHWRDRIVTKSPNGEEEKRAPLIFPEQDNNSRPQSTVKVCSRPEYESREQDATLASAFPHHMCQYLECPLSNAVGLCLHRLEVGCCSDIYSSNGGGSGCAGLNSEPESVYIYAESIKGCDSTCCSLLRECDSDCLYTEDPQREDGCYNTMQRKKSKIKKRLDSFYSNTEILCMYTEESSSFDMPDYSIEAVAGSDKSCNSIRSDCDSDSTSAYADWEHSFDSDGAEASMNSLDSLAEAQWGSVSSRLSSGSDASCYTADCGSESSYGTVEQSSSLVSEGSFGAGRCDSPVGYIQEDSCDLYNAWAENSDVDKNLYRSQTMDGATGFPCLQDDSELTLNELRGAVTHTERCHFSLFFREMVKEAAQDRDQEKLNVSEGFLFHPQKFLQAKNSEYREGREYSLLRNIQNGSYGDVFSIRDKQTGFTCAAKRIPLCSFSWEEVGTWSRLDSPQVLQLYGAVREGLNVVLFMDLKTGSLAQLLKARCSFPEDMALYYHSQVLQALEHLHSRQVIHLDVKVDNVLLSKDRRECFLCDFGLSEMLDKTGCSTKTFRGNGLRGTESHMAPEVARGDPRSDKADVWSSCCMLLHMLTGHQPWTRYYTHPLCLKIVSEPAPLWEIPPGCDPLTCEVIRAGLVKEPKERDSARKLLEKANKALQAVGGFSDPVLSHSSGTPELQNCPPVSLQVVPPLIALTTQETPPPLSAVAAPTSTSQQEFSAPRIQWVSTWRERAKEEDKTDSEERESDEESEWDSGGWTEEVKKDEMAVWMERYWGTEEDSSVGSNKKQMLLEGEREPCQKRGDDDDIYTERKKVSDNQEDHESGGGPGEADSEEMDDTDAAVDCILNEEGPYEGETRSSVDEAEGESAEEEVESDLESLRELGSDWAEEEWEPFHRLQILYGTKGQNKPYLKTEDDHSDTEEESEASTTASGKMTLHEPNSKLTLTCHRSTCASEPELTDKNSDWSDDLSSGVFSSYSSFTDGQSFNVDWSVSTHQPPSCCFEGLGVDIWVEDVSGETLRIRERLKVKLGHVAVGISAQISMRAFSLATLDGKLVSPDTEVLESGMWLQCVPASDSSANWDWRVRDGKLEKQEHDEGTSDTCSTLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEK5</accession>
    <name>A0A3B4CEK5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ZNFX1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017560736.2">
        <property type="nucleotide sequence ID" value="XM_017705247.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017560737.2">
        <property type="nucleotide sequence ID" value="XM_017705248.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037390967.1">
        <property type="nucleotide sequence ID" value="XM_037535070.1"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000010382"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017040.2">
        <property type="protein sequence ID" value="ENSPNAP00000010382.2"/>
        <property type="gene ID" value="ENSPNAG00000015929.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431837"/>
    <dbReference type="GeneTree" id="ENSGT00940000155154"/>
    <dbReference type="OMA" id="PVCQVPI"/>
    <dbReference type="OrthoDB" id="2423195at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17936">
        <property type="entry name" value="EEXXEc_NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000742">
        <property type="entry name" value="NFX1-type zinc finger-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001140">
        <property type="entry name" value="Zinc finger NFX1-type containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046439">
        <property type="entry name" value="ZF_RZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20173">
        <property type="entry name" value="ZnF_RZ-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51981">
        <property type="entry name" value="ZF_RZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RZ-type" evidence="9">
        <location>
            <begin position="1859"/>
            <end position="1930"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="896"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="834"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="73"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="896"/>
            <end position="908"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010382.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1947" mass="220041" checksum="F5EF562D6ED30975" modified="2024-11-27" version="2">MENRQRGGGNEQPRREPGQRGEHGMPNQERQRDGTGRRGGAGEGAGRGRGGAGGGGRGRGGAGGGGGRERDGAGGGGRGRGGAGGGGGGRGGGRGRGQGGGGREEAGRDRGERRGENRINRRPDIQRERNGGGVAQRGRGGRGEGEGQRGRGDHGRGNSRDGTPEVRKLGYKRLEELLEKDASVVAITLSSSAELKALLDDRTMRQDLVHLLCQVLRKAFQSKNDRTILLHLVQVVKDSAFFQTVLPHYVTGIMIDYSIPARREQYPLHLDNIICLLADVVSMFPYSSVRSVSMLLAVLKPGVNLLRASGIDICDTINENLEKIQGMVDHLQEKAREGTLRSDNYTLLIGNEDAPPDGEDFRNMTIYPTPEEFQQDQKPFLRPNIMSQSYPSAYAYLDTHFRLLREDFVRPLREGIRELLRSQRDETLGKVPMRKRRFDDIRVYFDTRVIVPLCTPTGTAYIVQFDARPLEFVRWENSKRLLYGSLVCLSMDNFETFLFATVSDRDPALLRKGAVQLSFSRDSRAELARIPASESFLMVETTAYFEAYRYVLEGLQEQVEEDLPFQRYIVECNQDVLAPAYLTQLRTTYDLSCIAVEDHKDNMKPFNPLIPNAWPTEEDLGLDESQLKAVKLALTKELAIIQGPPGTGKTYVGLKIAQALLKNSEVWSESTPMLVVCYTNHALDQFLEGIHGFLKKGIVRVGGRSNSEILKRFSLRELTRSSSFHRDLPPHLKKAYSEIQSEMRTAEVQIQRQSAQMECSLRGIINEHFLQQYISDEHWYSLNLLPMMDEFPHVGEKLSMMKEWLGLGNSVFQQAEQAEQAAANGNAGANEEGVAAEEEELLEMDEEAALIQAERIVVDAEPRERNARERKIEQEENLAELAEIMLAMNLDQIEEQPPQEQQQGQGQAADEEWKVQPHQKKKIKQMIQKELRKSSTMSEEEEEQVTNVWQLTLKNRWKLYRLWVLRYRIDLRTKSLEAEQVYQEAAERLSEIRRNEDLCVLRRAKVIGMTTTGAAKYRQALQALQPRLVVVEEAAEVLEAHTITTLSQDCQHLILIGDHQQLRPSATVYELAKNFNLEVSMFERLVTMDFPFVRLNYQHRMRPDIARLLTPHIYSELENHSSVLEYENIKGISTNLFFMDHKHLEEEIKDGRSHQNLHEAHFVVALCRYLLCQEYQPSQITILTTYTGQLYCLRKLMPSKEFTGVKVHVVDKYQGEENDIVILSLVRSNLVGKVGFLQIPNRVCVALSRAKKGLYCLGNMELFSKVKLWSNILHTLREKGQVGRALTLSCQNHPETRTLVSHAKDFAMVPEGGCNKPCEYRLDCGHVCIRMCHPYDADHKKYKCIKDCPKVLCELGHKCPRRCHQKCGDCMVRVEKVIPKCQHKQMIPCHQSPESFICKVPCQKTLSCGHLCQASCGEPCSAFCLVPVLMKLKCGHTQEEACSTSKDPTKQPPCRTKCNIVLKCGHPCSGTCHTCRQGRLHKACTYQCKSILPCSHECRVPCTRGCPPCTRPCENRCIHSVCKKKCGQPCVPCVEPCEWRCPHQSCTKLCHEPCDRPPCSEKCNKVLPCGHPCIGLCGDPCPKKCRICNHDEVTEIFFGSEDEPDACFIQLEDCGHIIESTAMDHYMGMDKDEEADLDQRAIRLKECPKCRKPIRRNVRYGAHVNRSQAEIDRVKVKIIGLQSDITQKQKDLKLFLERKVNFMQYLPAEYARVNENLQKSDLSLLDLWHQENLISFLERLGKLIKMQKDYMSFEKGQLFSLRVGECLKFLLDSTQRFSEQQVGDLESEMKRLAHLAELNIRCNKAGYTVTVMLVKVNAKVRQAREILEDNRPFTEENDLEVKRIFKELDAYLPCTGLGISDEERVMIVKAIGLTPGHWFKCPNGHVYAIGECGGAMQSGRCPECKATIGGANHTLTADNALATEMDGAQHAAWSEAVNLQNFDPMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEY7</accession>
    <name>A0A3B4CEY7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NLRC5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="42514.ENSPNAP00000010522"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017209.2">
        <property type="protein sequence ID" value="ENSPNAP00000010522.2"/>
        <property type="gene ID" value="ENSPNAG00000005083.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160652"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045345">
        <property type="term" value="P:positive regulation of MHC class I biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045348">
        <property type="term" value="P:positive regulation of MHC class II biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47189">
        <property type="entry name" value="MHC CLASS II TRANSACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47189:SF1">
        <property type="entry name" value="MHC CLASS II TRANSACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="791"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="818"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="203"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010522.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1492" mass="166799" checksum="49047DAAE77A12A1" modified="2024-11-27" version="2">MKMEEVDLEEDVMNVVAEEATVIVDVLCRERSNVLEQIYGLVERSTRDWLQSLPSLRERVLGIVDYFRASDPQKCCQFLQTMWMFCQSIPLDLETKVLSIAGSSSGVQGCFPENENSPRSCNVKRLCLDQVESYTNAVKLLLQQKFERVTKDVVKEVCLDEAWLCLRQRHSARVRDKSAQAQEGEEPSEHKESVESLLRTTGRVVVLLGQAGSGKTLLMHCLAHHWAQGFYPSIKLLFLLEFRQLNLVSQPLSLKELLFSFFLLPEGGVEQSEAVFSYILSNPEKICFIFDGYDEFGTKFTDPEKLPSPLGPYQQLSVTDLLSGICSYKILPKCTVLVTCRPRDVIDLFGSSGYFVAELLGFSQRRVKEYTETYFQEKGSELKERAVNLLMGNRHLLSMSHVPALCHVCCICLDHFLSEDTSQSHVKLPTSLTQIYLHILSAFLSRCQGGGSTNVSMPLLQKYRSQIAELSRLAMHGLENSRIVFLAQDLSTELMNFGANAGILSRVDLTCADGSHLGCAFMHLTMQEFLSALHLMTSPGISESQLKRKLNLKSRWTARTDPKTVFTDSLHLFMCGLAAKACRPSLALLEGSDDATAVVKKRQETVLKILRSFVGTASQTGPKIIELCRCAHETQDVSLAKDVASRTTFELRNIRLSPVDMDALAFVTSAADQMVCLDFGGCSIELECLSIIPNCKNLEHLIFRSRKYDDKFAEALSSILPDLQDLKQLHFISGGLTDTGTEKLIKALECCPQITHLNVSDNSLSDEVMYFSSLLCFVLNQTKALHNTNELLFNALLSLLLLLVVVAVVVVPIVVVKIVEVVVFFNYFVFRVFFSLTHSDIHPTDMNRLCKNLAKCKHLLELDFSHGTLKDESVERLQMLLPGMTSLQLLNLSYVQMSTDGALLLVRSLADCPRITTMELRQQGESFVRFRQITAGAATCKLIQYKLNGDNVKDLSGILEHCSYLADLDLSSNFLRDEDVKSFVNLLPRLQISNSLSLSNNKLTQLGALYLVNSLNSCEKVAAFIFICNLTLKIQKDMMSSLIIIFCVNNGWSYCFSVRIEEPWIKAEAAVRLVSSCLDLNSHIREIRVEKTCFEISLEGLPSRILYFVNDCIEVFSALYCDFSFFSFGDCEVEGQHLTFLQSTLQKCSSLLKLHFSQTTLGTNGAEFFSSVLPCLKKLKTLSLHSKGEGLDEAVIFALQSSQKHLENLSLSHHVINDSEAAKLGSALQGWTQMRSLNLSHCTGWTAAGGYKLVCGLVHCITLEEMRLNKIQVEVVEGSGVLCLLACLQSLDGLEELELEGLRMGDQGIKELVKYIPKWTTLRKINLSENCVSDQAGEMLVKALSHCRALQQLHLFRNKLGHEFAVRLGQVLPSLPQLSELECLFCSLTAIGTSDLICIASCLKHCTSIESISLAWNECGNDVAEKLAEVLPQCSTLKKIDLEANNINITGAKALVKCLQCCPWIEVIRLWRNPIVKDDQILRDQRLNFSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CF14</accession>
    <name>A0A3B4CF14_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="25">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557834.2">
        <property type="nucleotide sequence ID" value="XM_017702345.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000010599"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017279.2">
        <property type="protein sequence ID" value="ENSPNAP00000010552.2"/>
        <property type="gene ID" value="ENSPNAG00000016300.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430119"/>
    <dbReference type="CTD" id="57987"/>
    <dbReference type="GeneTree" id="ENSGT00940000156348"/>
    <dbReference type="OrthoDB" id="1100386at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071603">
        <property type="term" value="P:endothelial cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035331">
        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043114">
        <property type="term" value="P:regulation of vascular permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22845">
        <property type="entry name" value="Gal_Rha_Lectin_LPHN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000011">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.610:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003924">
        <property type="entry name" value="GPCR_2_latrophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003334">
        <property type="entry name" value="GPCR_2_latrophilin_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF61">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02354">
        <property type="entry name" value="Latrophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01444">
        <property type="entry name" value="LATROPHILIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043859450" evidence="19">
        <location>
            <begin position="48"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="880"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="943"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="979"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1018"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1062"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1091"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="22">
        <location>
            <begin position="63"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="24">
        <location>
            <begin position="162"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="21">
        <location>
            <begin position="491"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="20">
        <location>
            <begin position="687"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="23">
        <location>
            <begin position="874"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="462"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1354"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1395"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1359"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1407"/>
            <end position="1426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010552.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1518" mass="170274" checksum="AEC9826D0E5C94E2" modified="2024-11-27" version="2">MDCCDCSDFRPNMRRMSKPSTMACSAWSLSTLCCLMFALAAIQCTEGFSRAALPFGLVRRELSCEGYPIDLRCPGSDVIMIESANYGRTDDKICDADPFQMENINCYLPDAYKIMSQRCNNRTQCIVITGSDVFPDPCPGTYKYLEVQYECVPYKVEQKVFVCPGTLKAVGDPSFLFEAEQQAGAWCKDPLQAGDKVYFMPWTPYRTDTLIEYSSLDDFQNSRQTTTYKLPHRVDGTGFVVYDGAVFFNKERTRNIVKFDLRTRIKSGEAIINNANYHDTSPYKWGGKTDIDLAVDENGLWVIYATEQNNGMMVLSQLNPYTLRFEATWETAYDKRSASNAFMICGVLYVVRSTYEDNENEVSKGLIDYIYNTKQSHGEFVDINFPNQYQYIAAVEYNPRDNQLYVWNNFYILRYNLVFGPPDPAHAPPISEVTSAPVLPRTTTTTTTTTTQQKGLVTTITTTGTRVGNKPPKPPAAPPRTTTPPSLDSFPPPERFCDSVERRDIIWPQTQRGMLVERPCPKGTRGTASYLCVLSTGAWHPKGPDLSNCTSHWVNQVAQKIRSGENAANLANELAKHTKGPIFPGDISSTMRLMEQLVDILDAQLQELRPSEKDSTGRSFNKLQKRERTCRAFMKAIVDTVDNLLRPEALKSWRDMNSTEQTHAATMLLDTLEEGAFVLADNLMEPAIVKVPASNIILDVYVLSTDGQVQDFKFPQSSKGGISIQLSANTVKLNSRNGVAKLVFALYKNLGQFLSTENATIKLGSEAHGRNLSVAVNSDVIAASINKESSRVFITEPVIFTLEHIDMQHYFNSNCSFWNYSERSMMGYWSTQGCRLIDSNKTHTTCSCSHLTNFAILMAHQEVLGPDGLHKLLLTVITRVGIVVSLICLGMCIFTFCFFRGLQSDRNTIHKNFCINLFIAELTFLIGIDMTEYRIDCSVIAGILHYFFLASFAWMCLEGVQLYLMLVEVFESEYSRKKYYYVSGYLLPAIVVGVSAVVDYRSYGTTKACWLRVDNHFIWSFIGPVTFIIMLNLIFLVITMYKMVKHSTSLKPDSSRLENIKSWTLGAFALLCLVSLTWSFGLFFINEKSVVLAYLFTIFNTFQGMFIFIFYCLLQKKVRREYSKCFRHTYCCGGLPTENSHSSTKTATTRTSARYSSGTQSRIRRMWNDTVRKQSESSFISGDINSTSTLNQGMTGNYLLTNPLLRPQGTNNPYNTLLAETVVCNTPTAPVFNSPVTYRETRHSMNNARDTSAMDTLPLNGNFNNSYSLRNGDYGDSVQVVDCGLGLDDAAFEKMIISELVHNNLRACGKSHRSESRINTKKVAGADVGSQDDIIVAETSSLVHLGTETNHIVGGLSLHPHHHHTHNPHHPRELEAPLIPQRTHSLLYHTQERARTEPAAPLPPHSYATQLPAQPQAQDSLQSPNRDSLYASLPNLRDSPSAESGPDTLEDLSPSKQSESEDVYYKSMPNLGASSQQLHAYYQISRGSSDGYIIPITKEGCIPEGDVREGQMQLVTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFP9</accession>
    <name>A0A3B4CFP9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RAB22A, member RAS oncogene family</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RAB22A</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017539046.1">
        <property type="nucleotide sequence ID" value="XM_017683557.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFP9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009696"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016166.2">
        <property type="protein sequence ID" value="ENSPNAP00000009696.1"/>
        <property type="gene ID" value="ENSPNAG00000015161.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411808"/>
    <dbReference type="CTD" id="57403"/>
    <dbReference type="GeneTree" id="ENSGT00940000157009"/>
    <dbReference type="OMA" id="SESHRTC"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000346">
        <property type="entry name" value="RAB31, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21976" checksum="ECE075F2D135225D" modified="2018-12-05" version="1">MSLRELKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQERFRALAPMYYRGSAAAIIVYDITKEESFQTLKNWVKELRQHGPPNIVVAIAGNKCDLSDAREVPEKDAKDYADSIHAIFVETSAKNAININEIFTEISQRIPVLDTGGSSGVRGFKLRREPSDTTRERACC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CH18</accession>
    <name>A0A3B4CH18_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573622.1">
        <property type="nucleotide sequence ID" value="XM_017718133.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CH18"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009764"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016238.2">
        <property type="protein sequence ID" value="ENSPNAP00000009764.1"/>
        <property type="gene ID" value="ENSPNAG00000015270.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439633"/>
    <dbReference type="CTD" id="571626"/>
    <dbReference type="GeneTree" id="ENSGT00940000157398"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07615">
        <property type="entry name" value="BAR_Endophilin_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000053">
        <property type="entry name" value="endophilin-A1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000021">
        <property type="entry name" value="Endophilin-A2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032469">
        <property type="entry name" value="Endophilin-A3_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF45">
        <property type="entry name" value="ENDOPHILIN-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="15">
        <location>
            <begin position="18"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="322"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="43416" checksum="B812F76CCAACB316" modified="2018-12-05" version="1">MSVAGLKKQFHKASQLLSEKISGAEGTKLDEDFMEMERKIDVTNKSVFELLTKTTEYLQPNPASRAKLGMLNTMSKIRGQVKTTGYPQTEGLLGDCMLKYGRDLGDDSAFGCALVDVGEAMKQMADVKDSLDISVKQNFIDPLQTLQDKDLKEIGHHLKKLEGRRLDFDYKKKRQGKIPDEEIKQAVEKFEESKELAERSMFNFLENDVEQVSQLAALIEAALEYHRQSSEILEDLNSKLQNRIASASSRPKKEFKPKSIMSSLEALDVTQQNGLSYSSSLKSTDIQVSHSVNGKTDHISYHPPMTHTWSESPRDSETELHMDQPCCRSLYDFEPENEGELGFKEGDIIILTNQIDENWYEGMINGESGFFPINYVEVLVPLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CID4</accession>
    <name>A0A3B4CID4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial proton/calcium exchanger protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Electroneutral mitochondrial K(+)/H(+)exchanger</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Leucine zipper-EF-hand-containing transmembrane protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LETM1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) + 2 H(+)(out) = Ca(2+)(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72199"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LETM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CID4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010581"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017276.2">
        <property type="protein sequence ID" value="ENSPNAP00000010581.1"/>
        <property type="gene ID" value="ENSPNAG00000016131.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183167"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099093">
        <property type="term" value="P:calcium export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000290">
        <property type="entry name" value="LETM1 and EF-hand domain-containing protein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059005">
        <property type="entry name" value="LETM1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF8">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26561">
        <property type="entry name" value="LETM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="15 24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 23" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Letm1 RBD" evidence="26">
        <location>
            <begin position="263"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="25">
        <location>
            <begin position="694"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="478"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="563"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00035046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="771" mass="88571" checksum="DEF0750C92E113B4" modified="2018-12-05" version="1">MALILFSRSWTPLMKPSNVRSLKNELRKGNLTEGACFSCTALRLAANSPDILRGTSATQTFPLRKLLYPDHTSVRHRSPATSSHLYCAILIPDSLPGHAYSTSHPTISQLYTTPVRWVHTTGCLHDDSKVERSLRSLKDRNKKLEEGGPVYSPTVDAEPVRRTLRQRVVDEIKHYYHGFRLLWIDTTIAGRMLWRVLNGHALSRRERRQFLRTCADVFRLLPFLVFIIVPFMEFLLPVALKLFPNMLPSTFETQSKKEERLKKELRVKLEMAKFLQDTVEEIALRNKADKGNVTEEFSTFFQKIRDSGEIPSNEQIIRFSKLFEDELTLDNLTRPQLVALCRLLELQSIGTNNFLRFQLIMKLRAIRADDKLIAEEGVDSLNVNELQAACRVRGMRALGVTEDRLREQLKQWLELHLNQHIPTSLLLLSRAMFLPDTLSPADQLKTTLQTLPEIVAKEAQMKVAELDFSKVDNKTKLETMLQEEAAILQENREKEMERLADAAEKAKEQQKAEVEADIALPNVDVDVHSETLRDAAPVLEGIKFEQWQKYLRLQGEEITKEEIDMLSDACTKLKEQKKLLTKEKEELEELKDDVQEYSEDLEEIKKELSKTGQEKEVEESKASKHLSRRVNRMISRMDKIINELEKDKLVLDGQIDSGTTPPVGLFFEKRSDLQRENLISINELITIMRQIQSIPEQKLLSIADALDENKDGKIDIDDVMKVVELIDKEDIDISTTQVAEIMVMLQKEEKLVGKEKAKEKAEKEQAAKVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIH2</accession>
    <name>A0A3B4CIH2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017573613.1">
        <property type="nucleotide sequence ID" value="XM_017718124.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CIH2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010299"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035662.2">
        <property type="protein sequence ID" value="ENSPNAP00000010299.2"/>
        <property type="gene ID" value="ENSPNAG00000015841.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439627"/>
    <dbReference type="CTD" id="797538"/>
    <dbReference type="GeneTree" id="ENSGT00940000154484"/>
    <dbReference type="OMA" id="QDPWYTI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050795">
        <property type="term" value="P:regulation of behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046883">
        <property type="term" value="P:regulation of hormone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000248">
        <property type="entry name" value="5-hydroxytryptamine receptor 1A-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000610">
        <property type="entry name" value="5HT1A_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF191">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00512">
        <property type="entry name" value="5HT1ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0085">Behavior</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="52"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010299.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="409" mass="45785" checksum="B4C46BF00F2E9CFD" modified="2024-11-27" version="2">MEEEHNLSSLFQNDSGFYYQNDTVTVSAKVPLSYQVSTSLLIGALILCSVFGNACVVAAIALERSLQNVANYLIGSLAITDLMVSVLVLPMACLYQVLDKWTLGQVTCDIFISLDILCCTSSILHLCAIALDRYWAITDPIDYMKKRTLKRAAVLISVTWLVGFSISIPPMLIMKSQPKSKAEDIANPEACTISHDPWYTIYSTFGAFYIPLILMLVLYGRIFKAARFRIRKTVRKSEKVKCLTVSPALLKKSNGELKSWRSSVEPKPDACANGAAKRADDGESLEIVEVQGTKSTLPLPSAASQVPLFENRHEKNTEAKRKLALARERKTVKTLGIIMGTFIVCWLPFFTKALVMPFCPSCQMPVWLRDVINWLGYSNSLLNPIIYAYFNKDFQSAFKKIIKCHFCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNR0</accession>
    <name>A0A3B4CNR0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNR0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013682"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021351.2">
        <property type="protein sequence ID" value="ENSPNAP00000013682.1"/>
        <property type="gene ID" value="ENSPNAG00000019619.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163390"/>
    <dbReference type="OMA" id="TILITYH"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF176">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="466"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="526"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="555"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="595"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="626"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="665" mass="76113" checksum="7972170B5CE9D000" modified="2018-12-05" version="1">MKPLDLWFRFICSLSVIFLSVSGFYLPGLAPVSFCEEDKKTGDCQSEIQLFVNRLDSVESVLPYEYDVFDFCQDEEEKRPSENLGQVLFGERIETSPYRFTFKKDEACQKVCTKSYDTAKLEDKANLDFLKRGMQLNYQHHWIVDNMPVTWCYDVEDNQKYCNPGFPIGCLVTAEGRPKDACVINSEFNKKNTFYVFNHVDITITYHSGEHENWNGARLVSARLEPKSVKHSSENSQTCQGPPMEIPGEFSSNLNLTYSYSVTFESTDLIKWASRWDYILVSMPHTNIQWFSIMNSLVIVLFLSGMVAMIMLRTLHKDIARYNQVDQANMVKIHRQTGVAYEDAQEESGWKQVHGDVFRPPQKGMLLSVFLGQGTQIFIMTFITLFLACLGFLSPANRGALMTCAVVLWVLLGTPAGYVSARLYKSFGGEKWKTSVFLTALLCPGIVFADFFLMNLILWVEGSSAAIPFGTLVAVLGLWFGISVPLTFVGAYFGFKKPVIEHPVRTNQIPRQIPEQSFFTKPVPGIIMGGILPFGCIFIQLFFILNSIWSHQMYYMFGFLFLVFIILVITCSEATILLCYFHLCAEDYHWWWRSFLTSGFTAVYLFVYAIHYFFSKLQIQGAASTILYFGYTMIMVLIFFLFTGTIGFFACFWFVHKIYSVVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPE3</accession>
    <name>A0A3B4CPE3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Neuronal cell adhesion molecule</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NRCAM</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000036639.2">
        <property type="protein sequence ID" value="ENSPNAP00000012666.2"/>
        <property type="gene ID" value="ENSPNAG00000018713.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155419"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd05731">
        <property type="entry name" value="Ig3_L1-CAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000057">
        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000363">
        <property type="entry name" value="neurofascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000038">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000078">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000114">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000238">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000347">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000100">
        <property type="entry name" value="Neuronal cell adhesion molecule a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026966">
        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF15">
        <property type="entry name" value="NEURONAL CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043792207" description="Neuronal cell adhesion molecule" evidence="15">
        <location>
            <begin position="26"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1179"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="42"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="141"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="264"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="357"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="450"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="541"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="645"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="742"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="844"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="954"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="1074"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="729"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1209"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1209"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1288"/>
            <end position="1300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012666.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1313" mass="146106" checksum="ACCD150E52C2B35D" modified="2024-11-27" version="2">MLMMDRPRGCRTLLLVLFLGNMATALEVPLDPKVLEGLPQPPTITHQSPKDYIVDPRENIVIHCEAKGKPHPSFSWTRNGIHFDIDLESKVTMKPHSGTLIIDISGGDKAEAYEGVYQCTARNEHGTAISNNIVIRQSRSPLWSKEKIKPIVVQEGVSLVLQCRPPAGLPPPIIFWMDNNFQRLPQSRRVSQGLNGDLYFSNVVTEDSRSDYICYARFPHTQTIQQKQPITVRVLNMDAINETMAAYLNDTDLFGDDPAEERRPSFLVPTGSTSSKMVLRGQVLELECIAEGLPTPEVTWSKVSGELPTKRAFFQNFQKTLQIMDVSEADAGEYRCTAKNRLGSVHHTISVTVKAAPYWISAPRNLVLAPLETGVLTCRARGNPKPTISWFMNGVPIENAPTDLTRKVEDDTIIFTEVQTGSSAVYQCNASNEYGYLLSNAFVNVLAEPPRVLTPLDKVYQVIMNKRALLDCASFGSPLPGIRWFKDSRSSVLDGDPFVFHENGTLEIPVAQAFNSGKYTCVATNSFGISENRVHLEVKEPTRILKQPEYKVVQRNRNVVFECKVKHDPSLIPTMTWLKDNGELPDDERFIVDSDSLTIMEVTENDKGTYTCIMNTTLDQDSASAQLTVVEATPTPAVIYEKPDAPTDLELTDPGARSVQLTWIPGDEHNSPIKKFLIQYEDALHDSGTWHNLTEVPGTKTTAHLKLSPYVHYSFRVIARNEIGYSLPSQPSHQYKTNPAAPDENPSEVKGMGTEPNNLVISWKPLTGLQSNGPGLQYKVMWKQMHVDEKWTSVTVANVSKFVVAGTPTFVPYEVKVQAVNNYGHGPNPAVIIGYSGEDLPVEAPEISRVLVQNSTLAEVNWRSVPAKSVRGKLQGYRVYYWKERSLHKHNGHGHPHHMEQQTLTFGENRSHGKLPGLHPYSLYKVNIRVFNGRGEGPASTSQQFETPEGVPGQPAFLRVTDNNLDSLTLEWGPPHEKNGRLTGYTLKYHSVNNSNELGHLEELTVPANETSITLHNLKYSTRYKFYFSAKTLKGPGPSITEEFATIMDTGIPASPYPLSPTSQFLRPPHHKALPVSPVFGNVSSSVGEDGALITWEYWGPEKHIYVEYIVENSNEDWQKEMVNGSQSHVLKGLKEGLAYKVRLVAKGHTDQTVHRSEELLVTLPAMASRQVDIATQGWFIGLMCAIALLILVLLIVCFIKRNKGGKYPVKEKEDAHADPEIQPMKDDDITFGEYSDNEDHKPLKGSRTPSSGTVKRDESDDSLVDYGEGGDGQFNEDGSFIGQYSGKTERDTVQGHESSEAPSPVNAMNSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CSE4</accession>
    <name>A0A3B4CSE4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EXPERA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TM6SF2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TM6SF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556848.1">
        <property type="nucleotide sequence ID" value="XM_017701359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CSE4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022140.2">
        <property type="protein sequence ID" value="ENSPNAP00000014278.1"/>
        <property type="gene ID" value="ENSPNAG00000020190.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429540"/>
    <dbReference type="CTD" id="791179"/>
    <dbReference type="GeneTree" id="ENSGT00390000012913"/>
    <dbReference type="OMA" id="PELQEPY"/>
    <dbReference type="OrthoDB" id="8181520at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21106">
        <property type="entry name" value="TM6SF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047195">
        <property type="entry name" value="TM6SF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059044">
        <property type="entry name" value="TM_Tm6sf1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14568:SF13">
        <property type="entry name" value="SI:DKEY-19F23.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14568">
        <property type="entry name" value="TRANSMEMBRANE SUPERFAMILY 6 MEMBER 1/2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EBP"/>
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        <property type="entry name" value="TM_Tm6sf2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
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        <location>
            <begin position="58"/>
            <end position="183"/>
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    <feature type="domain" description="EXPERA" evidence="9">
        <location>
            <begin position="214"/>
            <end position="348"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034760"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="374" mass="42283" checksum="09316B1E4395C776" modified="2018-12-05" version="1">MVQEICVFFFSLTALGVLHIMNTVPAFQEPTVIMQTGLAVLLIVFLLIYLASRYNPPKDPLFYVFAEFCFTCVIDIVSALEYDGFISGYMDFYQRLGEPYLGSAYAIMMCYWDGIVHFIIYLVLIRRMSSRKPYRNLGLFWAGSLLANMTVFLMGIVVGKYGSEIYPAFWINMPFLLLPIWGAICLFQRPSDMPAVAVSKAVREQKKALIWRPLDLLLVIYLLGAMGFTLFRGLAVLDCPVEVFTSYTNQYEPYLKDPTGFPKVMMLLLLFHALPLFSLFVYGLIMPGCTWMLDWTVYVAGAILQTQWCHVGASLHPRTSEAYRAPESTLLPVLLLNLLYAVGPLLLALRCTGDPAYFLSTAPVGQANNEKKWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVS3</accession>
    <name>A0A3B4CVS3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVS3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015480"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023691.2">
        <property type="protein sequence ID" value="ENSPNAP00000015480.2"/>
        <property type="gene ID" value="ENSPNAG00000021544.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164374"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19800">
        <property type="entry name" value="Bbox2_xNF7-like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="TRIM/RBCC"/>
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    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
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        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
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        <property type="entry name" value="ZF_RING_1"/>
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        <property type="entry name" value="ZF_RING_2"/>
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    </dbReference>
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    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
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            <begin position="86"/>
            <end position="127"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015480.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="37447" checksum="F662400F36F35220" modified="2024-11-27" version="2">MALYKQIQCAICLCDFTDPVSLPCKHSFCRLCILGHLQACNGQSSCPECRQQFTKDDLQPSPLLRNVIEAIQGHSKEKQNRANLNTSGLVCAEHDERLRLFCETDQTLVRMICRDGTKHQGHQFKPISSQFEELHQFLRKKEEEIRRYGYGGEKIDEDILKSALNLNQPEMFLKICYTNFKKEALILKNLVEFPNTLFTVYYSCPPVAECFTISPRTPLIVSPDGLSVRRRDIDLDDHEDIYSNETVSAEETFTTGQHCWELEVGQKPDWTVGVEKRMEGSDLLARLTGWKRCISEVASDHGTTLEFTELLRETHSFTNVCRSSLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0F1</accession>
    <name>A0A3B4D0F1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Olfactomedin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">GLDN</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575002.2">
        <property type="nucleotide sequence ID" value="XM_017719513.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0F1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016526"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038155.2">
        <property type="protein sequence ID" value="ENSPNAP00000016526.2"/>
        <property type="gene ID" value="ENSPNAG00000022685.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440585"/>
    <dbReference type="GeneTree" id="ENSGT00940000158020"/>
    <dbReference type="OMA" id="YIYDHGY"/>
    <dbReference type="OrthoDB" id="8397025at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF85">
        <property type="entry name" value="GLIOMEDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="5">
        <location>
            <begin position="339"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="203"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016526.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="585" mass="64341" checksum="51DA7FECF7C29EA9" modified="2024-11-27" version="2">MKMLDPLAEARRGGSLRPCTGRLPWTLRWTLGSVCVLTLLNSAGLLLLLIQSRELGDRVAQAESRLEEIAQSSVVEFMTEMSRGQEEIQEELYQYSRNKRSQELRQEQVLEQLELEEKLAEQSGMGIREDLPKDLHREADHERKKIQEHPDLHPSQTHQRTQLHDGMMMMMTYSMVPVKVLLDICNSTKGVCLTGPPGPPGLPGVDGMPGYNGTDGIPGLPGEPGTPGKRGKKGPPGEKGDRGEPGEKGDSGPPGEKGEPSNDVIVEGPPGPAGPPGAPGPMGPPGLPGPPGPPRSGRNRNHRVHLNTDQTTEGHTGFLQTVPNDDQGPGNKVKASKNECIIKSVQNPSNLAKMTTTYGAWMKDTAVKDDEKIWVAEHFSGRIVKAYQSIAALQNSSSQSIDVRKFFQGCSHLVHNGYIYFHIAGTFNIAKLNLQTNRLHTLAIENALYHNLSYLLNNSKTYFKVAADESGLWLVFASSIDENIVVAQLDEKTFSITTYINTSYPRSKAGNAFIACGVLYVTDTKDTRVTYAFDLLKQKPVNVSFDLWSPSGVLAMLSYNPTDRHLYVWDGGYVKSYEVHFLSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D0W3</accession>
    <name>A0A3B4D0W3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D0W3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027023.2">
        <property type="protein sequence ID" value="ENSPNAP00000017947.2"/>
        <property type="gene ID" value="ENSPNAG00000024281.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175037"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF11">
        <property type="entry name" value="GALECTIN 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017947.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="188" mass="21257" checksum="66609A07D33460FC" modified="2024-11-27" version="2">MPSFSPLVSAKPQINISAKVGSLVLLPCDFSSKLSSIQKPHIEWSISSETVFERGGNESFQGYSYEDRVDVPEDKLSHGNGSLVLKDVRHADERIYKCKLKMKTGRQNKWVFIQAVSLSVEGKDVMLRGTDIRSSVYVGLYCYGLSCTENCYTNSSKCVECEQYSNVTFIFFVQNFQLPQNQLRRVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2N5</accession>
    <name>A0A3B4D2N5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">VDAC2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2N5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038694.2">
        <property type="protein sequence ID" value="ENSPNAP00000017815.2"/>
        <property type="gene ID" value="ENSPNAG00000024146.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183116"/>
    <dbReference type="OMA" id="HIAPNCA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF102">
        <property type="entry name" value="CEREBELLIN 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="6">
        <location>
            <begin position="69"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017815.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="207" mass="23455" checksum="99BFF8F35E1AF041" modified="2024-11-27" version="2">CVCHVCLTLPNINIIAEMKKLQDLEKRLKNVEEMLEEMKKEKEALLSFHLCTSTCMYVCICIYMYVYMFPEKKVAFSASLLASGEGYTGPMKPVPTPLIYKKVFSNYGNGYNPDTGVFTAPIKGVYFFRFYAHCQGGTTMAVSLYKNDERQCSVFSWKPAANGNATNGTVLTLEKGDRIYTKLWDETWVFDDTAGYTSFSGFLLFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D348</accession>
    <name>A0A3B4D348_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Coatomer subunit beta'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">COPB2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.</text>
    </comment>
    <comment type="function">
        <text evidence="14">This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasmic vesicle</location>
            <location evidence="1 15">COPI-coated vesicle membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Golgi apparatus membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
            <orientation evidence="15">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="15">The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578577.1">
        <property type="nucleotide sequence ID" value="XM_017723088.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D348"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018767"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028150.2">
        <property type="protein sequence ID" value="ENSPNAP00000018767.1"/>
        <property type="gene ID" value="ENSPNAG00000025316.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442844"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 15" id="KW-0931">ER-Golgi transport</keyword>
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    <keyword evidence="9 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="11"/>
            <end position="52"/>
        </location>
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            <begin position="319"/>
            <end position="577"/>
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    <feature type="domain" description="COPA/B TPR" evidence="19">
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            <begin position="594"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <end position="911"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="942" mass="105882" checksum="74FA145856030F6B" modified="2018-12-05" version="1">MPLRLDIKRKLTARSDRVKSVDLHPTEPWMMASLYNGSVCVWNHETQTLVKTFEVCDLPVRASKFVARKNWVITGADDMQIRVFNYNTLERVHMFEAHSDYIRCIAVHPTQPYILTSSDDMLIKLWDWEKKWSCSQVFEGHTHYVMQIVINPKDNNQFASASLDRTIKVWQLGSSSPNFTLEGHEKGVNCIDYYSGGDKPYLISGADDRLVKIWDYQNKTCVQTLEGHAQNVSCVSFHPELPIIVTGSEDGTVRIWHSSTYRLESTLNYGMERVWCVCGLRGSNNVALGYDEGSIIIKLGREEPAMSMDTNGKIIWAKHSEVQQANLKAMGDAEIKDGERLPLAVKDMGSCEIYPQTIQHNPNGRFVVVCGDGEYIIYTAMALRNKSFGSAQEFVWAHDSSEYAIRESNSVVKIFKNFKEKKSFKPDFGAEGIYGGFLLGVRSVNGLAFYDWENTELIRRIEIQPKHIFWSDSGELVCIATEESFFILRYLADKVAASQESNEGVTEDGIEDAFEVQGEIQEIVKTGLWVGDCFIYTSSVNRLNYFVGGEIVTIAHLDRTMYLLGYIPKDDRLYLGDKELNIVSYSLLVSVLEYQTAVMRRDFGMADKVLPTIPKEQRTRVAHFLEKQGFKQQALAVSTDPEHRFELALQLGELKIAYQLAVEAESEQKWKQLAELAISKCQFGLAQECLHHAQDYGGLLLLATASGNATMVSKLAEGAERDGKNNVAFMTYFLQGKLDQCLELLIRTNRLPEAAFLARTYLPSQVSRVVKLWRESLAKVNQKAAESLADPTEYENLFPGLKEAFVAEHYLRETCLGKTRPATDYPLVTPNEERNVLEEASGYEPKGVPPTLIQKVEDTQEATEEAGVLASVMAAVSAPIAAAVASVAPPEPEPTKKPEVATTIASTLQDKTLEELEDDLDNMELDDIDTTDVNLDDDFLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D539</accession>
    <name>A0A3B4D539_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D539"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018573"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027811.2">
        <property type="protein sequence ID" value="ENSPNAP00000018573.1"/>
        <property type="gene ID" value="ENSPNAG00000024998.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183023"/>
    <dbReference type="OMA" id="ICIIRAC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF871">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="315" mass="36396" checksum="647B982B7C1F00C7" modified="2018-12-05" version="1">MENSSRELMFVLHGLSETRTNKYIYFAFGLVVYIVTLLVNLTLIIAIILDKTLHEPMYLFICNLFVNGICGASAFYPKILADLFSDLHVISYTGCLAQIYIICCYIFCEITCLTVMAYDRYVAICRPLEYYTIMNKQKVMKLLIFMWFFSVLETSVGIVLTARLPLCGNDIDKIYCSNWEVVKLSCTDVTVNNIYGYLTFYHVCQAAFIFVSYIRIIRASLRSKTERVKFMQTCLPHLITLINFTISVLFDVLYARYGRSLGLQALRNILGMEFLVVPPLLNPIIYGLKMTQIRHLKMYNSKQCNKTEIYSRITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D715</accession>
    <name>A0A3B4D715_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SERPINI1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D715"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018609"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028072.2">
        <property type="protein sequence ID" value="ENSPNAP00000018724.2"/>
        <property type="gene ID" value="ENSPNAG00000025048.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158168"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.310.10:FF:000001">
        <property type="entry name" value="Serpin family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000005">
        <property type="entry name" value="serpin I2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF50">
        <property type="entry name" value="NEUROSERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043691522" description="Serpin domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="10">
        <location>
            <begin position="33"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018724.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="420" mass="47242" checksum="B37FD75C8AAD7867" modified="2024-11-27" version="1">MHVCVCLVPLLLLCESSCRAADVPEDVTAEFSVRLYHQLQMVGGEDNIIFSPLSVALALGMVELGARGSSLQEIRQAVGYSHFREDEEFSMLRNVTQALSVDEAQYIIRLANSLFLQTGVHFSSEFLRLMKQYFHADAETVDFSESAAVAQHINTWVQNHTESKIYDLVSADDFSSVTRITLVNAVYFRGSWKNQFRPENTRTFSFSKDDGSEVQTLMMYQQGDFYYGEFSDGSAEAGGVYQVLEMPYEGEDLSMMIVLPRQEVPLATLEPIIRPQLLDEWANNVKKQKVEVYLPRFKVEQKIDLKDTLQQLGIKNIFTKDADLSAMTAERADGSDLFIGKAVQKSYLEVTEEGAEGAAGSGMIALTRTLVLYPQVMADHPFFFVIRNRRTGSLLFMGRVMNPEVVDPFDQDLDSADTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D823</accession>
    <name>A0A3B4D823_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Biliverdin reductase A</fullName>
            <shortName evidence="1">BVR A</shortName>
            <ecNumber evidence="1">1.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">BLVRA</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Does not reduce bilirubin IXbeta. Uses the reactants NADH or NADPH depending on the pH; NADH is used at the acidic pH range (6-6.9) and NADPH at the alkaline range (8.5-8.7). NADPH, however, is the probable reactant in biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4Z,15Z)-bilirubin IXalpha + NAD(+) = biliverdin IXalpha + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15797"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57977"/>
            <dbReference type="ChEBI" id="CHEBI:57991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4Z,15Z)-bilirubin IXalpha + NADP(+) = biliverdin IXalpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57977"/>
            <dbReference type="ChEBI" id="CHEBI:57991"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoheme degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.24" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017555749.1">
        <property type="nucleotide sequence ID" value="XM_017700260.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017555750.1">
        <property type="nucleotide sequence ID" value="XM_017700261.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D823"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019099"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028707.2">
        <property type="protein sequence ID" value="ENSPNAP00000019099.1"/>
        <property type="gene ID" value="ENSPNAG00000025610.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428891"/>
    <dbReference type="CTD" id="644"/>
    <dbReference type="GeneTree" id="ENSGT00390000011072"/>
    <dbReference type="OMA" id="IQVAFIC"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="UniPathway" id="UPA00684"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106276">
        <property type="term" value="F:biliberdin reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106277">
        <property type="term" value="F:biliverdin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042167">
        <property type="term" value="P:heme catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017094">
        <property type="entry name" value="Biliverdin_Rdtase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015249">
        <property type="entry name" value="Biliverdin_Rdtase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09166">
        <property type="entry name" value="Biliv-reduc_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037032">
        <property type="entry name" value="Biliverdin_reductase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="1 2" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Biliverdin reductase catalytic" evidence="5">
        <location>
            <begin position="128"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR037032-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR037032-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="292" mass="32569" checksum="C9A676AEE650043F" modified="2018-12-05" version="1">MLGSVVVGVGIAGSVRIRDLLAPLSSSAAEKLTVKGFVSRRTLPEQHGVQQISIEEALSRNDIQVAFVCTENSSHEEHIRQFLNAGKHVCVEYPMALSYSSAADLWDLAQQKGLVLHEEHIELFTPDYKQLKKDIAGKKLEEGSLHFTGGSLKPDFGFPSFSGIARLTWLVDLFGELTVKEATLVEDEERKYMKLTAHFLTKDQKPLTWVEERGVGMGRAKHIHLRFDSCTLTELPAGQREPVGLFMQDLVLFGRKLQGEVPTEELQAEQSRILHCLQLAENIQQFCQSTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8F3</accession>
    <name>A0A3B4D8F3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">COPS8</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8F3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019708"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039608.2">
        <property type="protein sequence ID" value="ENSPNAP00000019708.1"/>
        <property type="gene ID" value="ENSPNAG00000026378.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440484"/>
    <dbReference type="CTD" id="10920"/>
    <dbReference type="GeneTree" id="ENSGT00940000166277"/>
    <dbReference type="OrthoDB" id="5351233at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010387">
        <property type="term" value="P:COP9 signalosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000011">
        <property type="entry name" value="COP9 signalosome complex subunit 8-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033205">
        <property type="entry name" value="COP9_CSN8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13339">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13339:SF0">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="54"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="239" mass="26470" checksum="C02C3EB8695E28B9" modified="2018-12-05" version="1">MNPFTVDWLAPRSLPVGVSVVWSLCARAREEVAAPFAHRLAAKAAPARAMPVAVTMGDYGKLLEQSETQELEAPGGIATPQVYSQLLALYLLHNDMNNGRYLWKRIPQAIKAANPELAAIWAVGQRIWQRDFPGIYSAIAAYQWSETILPVMESLRESTRRRAYGLVAQAYTSISAEDFASFVGYSVEEAVKGVVSHGWQADPNTRMIMPQKPDPPPVSLVPNEQQLARLTDYVAFLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D9H6</accession>
    <name>A0A3B4D9H6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM167 (SEC) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566014.1">
        <property type="nucleotide sequence ID" value="XM_017710525.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017566015.1">
        <property type="nucleotide sequence ID" value="XM_017710526.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017566017.1">
        <property type="nucleotide sequence ID" value="XM_017710528.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393352.1">
        <property type="nucleotide sequence ID" value="XM_037537455.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393353.1">
        <property type="nucleotide sequence ID" value="XM_037537456.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393354.1">
        <property type="nucleotide sequence ID" value="XM_037537457.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393355.1">
        <property type="nucleotide sequence ID" value="XM_037537458.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393356.1">
        <property type="nucleotide sequence ID" value="XM_037537459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D9H6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030434.2">
        <property type="protein sequence ID" value="ENSPNAP00000036214.1"/>
        <property type="gene ID" value="ENSPNAG00000026883.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435018"/>
    <dbReference type="CTD" id="567341"/>
    <dbReference type="GeneTree" id="ENSGT00940000159097"/>
    <dbReference type="OMA" id="YNTMATQ"/>
    <dbReference type="OrthoDB" id="5965452at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024280">
        <property type="entry name" value="FAM167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051771">
        <property type="entry name" value="FAM167_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32289">
        <property type="entry name" value="PROTEIN FAM167A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32289:SF3">
        <property type="entry name" value="PROTEIN FAM167A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11652">
        <property type="entry name" value="FAM167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="209" mass="23519" checksum="E478E58BBB8CB590" modified="2018-12-05" version="1">MDTGTIPQINVEDISGLDYDNLEVATDDHLRTLKALAEKLRLETRRPSYLEWKAQVEAQSAKAPSEPAECSEPGQAVGQEHADRPERNGQNSDLIQESDLTSGSLKGFGNIDEALIWLRKELKEMRLQDQQLARQLMRLRGDINKLKIEQTCHLHRRMLNDATFGLEEHDELSDLLCDGPVTPGFGLSAPLRLIGVTKMNINSRRFSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCF8</accession>
    <name>A0A3B4DCF8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ceramide transfer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Collagen type IV alpha-3-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-hexadecanoylsphing-4-enine(in) = N-hexadecanoylsphing-4-enine(out)</text>
            <dbReference type="Rhea" id="RHEA:45720"/>
            <dbReference type="ChEBI" id="CHEBI:72959"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017546606.1">
        <property type="nucleotide sequence ID" value="XM_017691117.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCF8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021165"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032205.2">
        <property type="protein sequence ID" value="ENSPNAP00000021165.1"/>
        <property type="gene ID" value="ENSPNAG00000028148.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423664"/>
    <dbReference type="CTD" id="796455"/>
    <dbReference type="GeneTree" id="ENSGT00940000155123"/>
    <dbReference type="OMA" id="LETCHRI"/>
    <dbReference type="OrthoDB" id="2344588at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13283">
        <property type="entry name" value="PH_GPBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08872">
        <property type="entry name" value="START_STARD11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000104">
        <property type="entry name" value="collagen type IV alpha-3-binding protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000003">
        <property type="entry name" value="Collagen type IV alpha-3-binding protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041952">
        <property type="entry name" value="STARD11_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF53">
        <property type="entry name" value="CERAMIDE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="16">
        <location>
            <begin position="408"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="197"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="617" mass="70370" checksum="29428AC255223C0B" modified="2018-12-05" version="1">MSDQSSSPTSSDEDVDPESEQTDELGGTLSKWTNYIHGWQDRWVVLKNNTLSYYKSEDEREYGCRGSLCLSKAVITPHEFDECRFDVSVNDSVWYLRAEDPDLRQQWIDTIELHKAESGYGSESSLRHHGSMLSLTSAASGLSTTSTSSFRKGHSLREKLAEMETFRDILCRQVDTLQRYFDNCASGDYRDEYHRDKVEDDEDDFPSTRPDGDYLLNNNSSKEKLFPMTRGPTGIDFKGEAITFKATTAGILSTLSHCIEIMVKREESWQKRLDKEMERRRRAEEAYKSALTELKKKSHYGGPDYEEGPNSLINEDEFFDAVEAALDKQDKFEEQSEKCRAPRPITLSPDACCSISAQRYFAKPNSHASSLSSLDLVSASDDVHRFSAQVEEMVHSHMTYSLQDVGGDANWQLITEEGEMKVYRREVEENGVVLDPLKATHAVRGVTGHEVCHYFWDTAFRNDWETTVEHFNVVETLSDTAVIIYQTLKRVWPASQRDILYLSVIRKILSTNENEPNTWLVCNFSVDHDSYPPTSRCIRAKINVAMICQTLVSPPEGNKEISRDNILCKITYVANVNPGGWAPASVLRAVARREYPKFLKRFTSYVQEKTSDDAILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCY5</accession>
    <name>A0A3B4DCY5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cilia- and flagella-associated protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CFAP45</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP45 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557460.1">
        <property type="nucleotide sequence ID" value="XM_017701971.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCY5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021345"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032542.2">
        <property type="protein sequence ID" value="ENSPNAP00000021345.1"/>
        <property type="gene ID" value="ENSPNAG00000028452.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429899"/>
    <dbReference type="CTD" id="25790"/>
    <dbReference type="GeneTree" id="ENSGT00730000111174"/>
    <dbReference type="OrthoDB" id="1902038at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033253">
        <property type="entry name" value="CFAP45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15504:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15504">
        <property type="entry name" value="NASOPHARYNGEAL EPITHELIUM SPECIFIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="10">
        <location>
            <begin position="215"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="407"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="200"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="580" mass="68387" checksum="AB9ABC08D4D182FD" modified="2018-12-05" version="1">MELLCPMDIDTTRDWIDPAFLPNQVMWRTLQPVPRSATSSSARLSCGGSSSSSGARSRRYRTRALTSQVDESLFGAPKQSACELKDEAKGSRALSHSAPASKSQKTETVRIITKDLIRDLRIRNRDPSGQSIIICTEEIGRITAESHAPAKEEREASLESQRRFREEAVNAAEERKTLMHQADLSRQKNQGLSDLEAEARQRAQYLLERANALRMEQEDEVKKLNELILGAQCHAVREAQILERKQVLKELQEEERRLDAMMEVDRRRALELQEQIDQLCKQQRIQGKMCILKQIEERLEERMLDDEVKEQEGQQMLESLERMQMEELEAMERKKAEQRRLLLEIQKINEENLLAKEHKKEEERLADLRALEYTSKKLEREAEYEAEQKLIKREKEKEVARLRALQERDRDHKAEQDELRARRNQEAAEREWRRKEKEQARKKLEEEDRLKAARLEQVTHKEHMLSIEAGRERAEFERVLRTQQELIAREKEKEEGHRQQVLRHAEGVRQQIREREMLAIARRREIFREGEKLDEESRSRRVRLDEIKDKKLKELRAAGLPEKYCNEVERKIQALPTVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEN3</accession>
    <name>A0A3B4DEN3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microtubule-associated protein 1 light chain 3 alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MAP1LC3A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548326.1">
        <property type="nucleotide sequence ID" value="XM_017692837.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEN3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022872"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014065.2">
        <property type="protein sequence ID" value="ENSPNAP00000022872.1"/>
        <property type="gene ID" value="ENSPNAG00000007430.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424667"/>
    <dbReference type="CTD" id="84557"/>
    <dbReference type="GeneTree" id="ENSGT00940000158853"/>
    <dbReference type="OMA" id="VNERSMV"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17234">
        <property type="entry name" value="Ubl_ATG8_MAP1LC3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000059">
        <property type="entry name" value="Microtubule-associated proteins 1A/1B light chain 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="11">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="14342" checksum="01F8963354A32CFF" modified="2018-12-05" version="1">MPSDRPFKQRRTFADRCKEVQQIREQHPNKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPISEIYEQERDEDGFLYMVYASQETFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEU8</accession>
    <name>A0A3B4DEU8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TSG101 and ALIX binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEU8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022025"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033858.2">
        <property type="protein sequence ID" value="ENSPNAP00000022025.2"/>
        <property type="gene ID" value="ENSPNAG00000029342.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047961"/>
    <dbReference type="OMA" id="CELHATM"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1180">
        <property type="entry name" value="Geminin coiled-coil domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038926">
        <property type="entry name" value="CEP55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022008">
        <property type="entry name" value="EABR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31838">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 55 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31838:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 55 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12180">
        <property type="entry name" value="EABR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TSG101 and ALIX binding" evidence="6">
        <location>
            <begin position="162"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="129"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="196"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="346"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022025.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="456" mass="53210" checksum="92234038171523A9" modified="2024-11-27" version="2">MPAGLILFYRLSTMASKFLRGKTVEKSPSPQTQREIGQLREENADLKKTLDELGRQKGAPSDSENSKLLERILALETLREKNSQQILSRDQEIASLRQQLRPDTAEVVAGLQAQLNQQRHESEMREKLFQSLKQETEEVKNKLAAVSAKCQDLESRQEQAEEHLKDALEKNLQWLAYDQQREAYVKAVLERTCILEQQLNQANEALQQKDKEASSEGRLQAMETQRCYEELLRSARAELEEERERVAQGRRLLSQLQQQCEERQREVLEMRQQLQAVHLSCKNELKEERRHIERLRAELDGVTARLEDERLRSAELRQQVLESLMDVFHPSVLRQGNNYLFTHPQIQLSAKDLEDEKRDNQHLQRQLHKILKELRKAKDRVARLECENAQREPPSSELRAYSKVEKACKEGQQSFISPTRAHSLLDESFLECPNCRAQYPTSRHRELLVHIDQCLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJ61</accession>
    <name>A0A3B4DJ61_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin carboxyl-terminal hydrolase 15</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Deubiquitinating enzyme 15</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin thioesterase 15</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquitin-specific-processing protease 15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">USP15</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJ61"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023166"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013320.2">
        <property type="protein sequence ID" value="ENSPNAP00000023166.2"/>
        <property type="gene ID" value="ENSPNAG00000007714.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154932"/>
    <dbReference type="OMA" id="PCHAQQS"/>
    <dbReference type="OrthoDB" id="265776at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000003">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000013">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000034">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000020">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 15 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028135">
        <property type="entry name" value="Ub_USP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF28">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14836">
        <property type="entry name" value="Ubiquitin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="18">
        <location>
            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="17">
        <location>
            <begin position="290"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="625"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="222"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="655"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023166.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="936" mass="107127" checksum="0E3E7097BAFE8640" modified="2024-11-27" version="2">MAEGGAADLETQRGEIAALLKTQLRKGDTWYLIDSHWFKQWKKYVGFDSWDKYQMGDQNVYPGPVDNSGLLKDGDVLAIKEHLIDELDYILVPTEGWNKLVSWYGLTESQEPIARKVVEQGMFVKHCKVEVYLTELKLCEDSNMDNIVTRRFSKADTIDTIEKEMRKLFSIPDERETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGSSTPKSSGASNLSALPKISPTSLTNNHNSSFSSRNVKNSSYSLLSYPPYNSYEYSDQSRHSEKEGLCGLSNLGNTCFMNSATQCLSNIPPLTEYFLKDKYQDELNEDNPLGMKGEIAKAYAELIKQLWSGKYSYVTPRPFKTQVGRFAPQFSGYQQQDSHELLAFLLDGLHEDLNRIRKKPYIQLKDAEGRPDKVVAEEAWENHIKRNDSIIVDIFHGLFKSTLVCPVCSKVSVTFDPFCYLTLPLPMKKERTLEVYLVRLDPLAKPTQYKLTVPKVGYISDLCASLSALSGVPAEKMIVTDIYNHRFHRIFATNENLSSIMERDDIYVFEVAVNRLEDTDHVVIPVHLREKYKQSGYNHSSTLLFGQPFLITVPRTLSEEKFYNLLLLRLCRFVRSALEEEESDETHSSKQHAINGNATNGILEEGSPSEMETDEQDDESSQDQELPSENDNSQSEDSVGGDNDLENGVGPETLTKGQQLTGHKKRLFTFQFNNMGKTDLSFIKEEPRQIRFDEGHLRLSDRSYLSLDWEPEIKKKYFDESVAEDFDKHESMEYKPQKKAFFKLKDCIELFTTKEKLGAEDPWYCPSCKQHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDSLVDFPLRDLDMSEFLINPNAGPCRYDLIAVSNHYGGMGGGHYTAYAKNKDDGKWYNFDDSIVSPVSEDQIVVRQFLHLASSTALFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKA9</accession>
    <name>A0A3B4DKA9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GSC</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017566759.1">
        <property type="nucleotide sequence ID" value="XM_017711270.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKA9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023910"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011462.2">
        <property type="protein sequence ID" value="ENSPNAP00000023910.1"/>
        <property type="gene ID" value="ENSPNAG00000000960.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435432"/>
    <dbReference type="CTD" id="145258"/>
    <dbReference type="GeneTree" id="ENSGT00940000160635"/>
    <dbReference type="OMA" id="QCSCVPA"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000210">
        <property type="entry name" value="homeobox protein goosecoid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051440">
        <property type="entry name" value="Goosecoid-like_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="140"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="195"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="236" mass="26107" checksum="A1F0CA83C6F37171" modified="2018-12-05" version="1">MPAGMFSIDSILAGRPSCKDPALLQRSAPLVFSNLADSLYSAADYNGLYSPTGAAAASLPPVSGARLGYNSYCYGQLHVQGPSGPACCGAMPTQCPCIPTGYDSGGSVLMSPVPHQMVSYLNMGSLSRTDLHLLSQLQCRRKRRHRTIFTDEQLDALERLFQETKYPDVGAREQLARKVHLREEKVEVWFKNRRAKWRRQKRSSSEESENTQKWSKPTKSAAEKTEESKSDADSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKR4</accession>
    <name>A0A3B4DKR4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibroblast growth factor</fullName>
            <shortName evidence="5">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568837.1">
        <property type="nucleotide sequence ID" value="XM_017713348.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKR4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024967"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008784.2">
        <property type="protein sequence ID" value="ENSPNAP00000024967.1"/>
        <property type="gene ID" value="ENSPNAG00000001336.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436698"/>
    <dbReference type="CTD" id="359830"/>
    <dbReference type="GeneTree" id="ENSGT00940000158907"/>
    <dbReference type="OMA" id="DCHLIER"/>
    <dbReference type="OrthoDB" id="10008525at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035118">
        <property type="term" value="P:embryonic pectoral fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048919">
        <property type="term" value="P:posterior lateral line neuromast development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048920">
        <property type="term" value="P:posterior lateral line neuromast primordium migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902036">
        <property type="term" value="P:regulation of hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060021">
        <property type="term" value="P:roof of mouth development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048794">
        <property type="term" value="P:swim bladder development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23306">
        <property type="entry name" value="beta-trefoil_FGF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000004">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017099539" description="Fibroblast growth factor" evidence="5">
        <location>
            <begin position="33"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="212" mass="23495" checksum="06DD579D06A1B957" modified="2018-12-05" version="1">MCKWTVTKGAAAWFRPPRVCALLLLLALLCSSLPVACHGARSSRPARSPPPSRVATNSSSSAVAAVVVGRHVRSYSHLTGDVRKRKLFSYQKFFLRIDKNGTVNGTKSKDDPFSILEIKSVDVGIVAIKGLDSNYYLAINKKGAVYGAREFGIDCKLIERIEENRYNTYASAEWRNKKKPMFVGLSANGKPMRAKKTRRKNTATHFLPIPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DX01</accession>
    <name>A0A3B4DX01_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017579837.1">
        <property type="nucleotide sequence ID" value="XM_017724348.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DX01"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002217.2">
        <property type="protein sequence ID" value="ENSPNAP00000027586.1"/>
        <property type="gene ID" value="ENSPNAG00000013097.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443578"/>
    <dbReference type="GeneTree" id="ENSGT01030000235274"/>
    <dbReference type="OMA" id="NEYFHET"/>
    <dbReference type="OrthoDB" id="8786186at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017408677" evidence="1">
        <location>
            <begin position="21"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="534" mass="62348" checksum="6314C5EB0A1B0150" modified="2018-12-05" version="1">MDKLWVILTLAGLLTSGAESFKISEKEYENIDRMLEVGVQIFLNCLDKVKEATEKLVKEDMLRTNRIAIQDNWNSFRETANSISVEERGKGVQDDLIVAARTVRRLVLHYKDDVKKIFHTVIMEQNVQLYLPVVNMFKGTIERAKVNEIWGNLVSNYLPGAPSIESEPLEFLCYLLSTSTVETYIVKVLKFGVEVISEIKKIDFDKVTERVRTIFKGVGEKCKEFERIEREKEKDDFLVMVSVTSRVFIFYWDSFLNATDTPELGEKYEDIKRKLMDLKFDEELKEALKPLMESETLNDILRLHFEFAAEFYFIGFSPLITLFITAPERFVRTVRVVGMSAVIHAYTIYEQLSERGLFSEMKRLYFRAVDIGIALAGNEVFQYFRETYFEKWKSFREELNKLVNEERRKSHILLPQVRAIARMLISKFEELVDKVNADFDDDTVQQMSTKWSNIKEKCIKKLLTSGALTQDQLEEAQRIFQEDAFGFYNKYLEFTKTMDPQRVEATLKALVKMLVLGVNFIYSPLAQAWEFLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3J9</accession>
    <name>A0A3B4E3J9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Ubiquitin-like protein that plays a role in cell proliferation and sister chromatid cohesion by associating with spliceosomal proteins. Participates thereby in pre-mRNA splicing by maintaining spliceosome integrity. Promotes the functional integrity of the Fanconi anemia DNA repair pathway by interacting with FANCI component and subsequently mediating the formation of FANCI homodimers. Also plays a protective role against ER stress-induced apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with CLK1, CLK3 and CLK4. Interacts with coilin/COIL. Interacts with spliceosome components SART1 and EFTUD2. Interacts with FANCI; this interaction promotes FANCI dimerization.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3J9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030268"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018147.2">
        <property type="protein sequence ID" value="ENSPNAP00000030268.2"/>
        <property type="gene ID" value="ENSPNAG00000016895.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001945"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd01791">
        <property type="entry name" value="Ubl_UBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000052">
        <property type="entry name" value="Ubiquitin-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="5">
        <location>
            <begin position="88"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00059610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030268.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="159" mass="18242" checksum="D97F66F8B9083E25" modified="2024-11-27" version="2">FTSCSYDTSANDIRSRQSVPPYFPRISDFIGHSLTSIKAFRASDWLWPLACLSEDGPRCLFAEERVLFSREKKTADSVKFTVGSAEMIEVVCNDRLGKKVRVKCNSEDTIGDLKKLIAAQTGTRWDKIVLKKWYTIFKDHVTLGDYEIHDGMNLELYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E889</accession>
    <name>A0A3B4E889_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">VPS9D1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017544784.2">
        <property type="nucleotide sequence ID" value="XM_017689295.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E889"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032000"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021498.2">
        <property type="protein sequence ID" value="ENSPNAP00000032000.2"/>
        <property type="gene ID" value="ENSPNAG00000019676.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108416248"/>
    <dbReference type="GeneTree" id="ENSGT00390000015057"/>
    <dbReference type="OMA" id="CLKPAMP"/>
    <dbReference type="OrthoDB" id="10264848at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF98">
        <property type="entry name" value="VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="2">
        <location>
            <begin position="522"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032000.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="686" mass="76670" checksum="E0EE678B95101AB7" modified="2024-11-27" version="2">MASVIVSGQRPLQNAMRLVRVAIQLDTGNRHKEAYCEYLKSINYISHALLQDAACKAEGEMEDLEVEKMVKLVEQCLERVKSCIGKRNDTSDPDSSATPVHGTPLTQITAHVLNVVASPTKSDETAMESKTQSKPSSRHRRVLSEGEGAGCPFLPPEVFQKLQIVDSQDSKKELTPIEEASRLNQKLKANYEARLARLAPSQATQKTSLTLSLQRQMMENLIIARARQDALQRKMEERRLRLQEQANRRFSSSGTLTPEEQEQRVLYANILEYEQDNEWPKVWKANLKKNPNDATLVSGLVTYLLSCPDHPVMKLLKKLQYQVYNKLYPIVSQSVPSSPLPGRSLSLKPSRSAQSLLFTDSPKGQLKSGLVHSLSDASISLSLVHDLNTTDTEPELDSEEPVTPAPPDRENSFEDLEQFLAQLGWNPYQSADETVSYVTCDSTHGDLEVHVLGLEERALKEHLKAIVKDIHNAIDRLLSLCLLSFECLNTASAKDHCVASIEEVFFTPIWSPLLALFRRVHQERELAFESSVQLYRNASPGDIGVASKLYPKNSALLHGSYPYESAVQELRLLCRDYSPQRKLECIVRTLRLICACAEHYRLLQENDPSPKTAAIGADDLLPILSFVALRTELPQLVSECAALEEFIHEGYLIGEEGYCLTSMQSALTYVESLHLGGIVQQAAKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ECC4</accession>
    <name>A0A3B4ECC4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Ruffle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000033485"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037962.2">
        <property type="protein sequence ID" value="ENSPNAP00000033485.2"/>
        <property type="gene ID" value="ENSPNAG00000022139.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155061"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13295">
        <property type="entry name" value="PH_EFA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000004">
        <property type="entry name" value="PH and SEC7 domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000054">
        <property type="entry name" value="PH and SEC7 domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF334">
        <property type="entry name" value="PH AND SEC7 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="9">
        <location>
            <begin position="1007"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="1216"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="54"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="124"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="427"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="562"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="645"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="748"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="948"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1444"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="520"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="573"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="599"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="761"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="779"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1444"/>
            <end position="1464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033485.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1498" mass="165650" checksum="5CD77F88DC9FA72D" modified="2024-11-27" version="2">MENCSTSTPPPKASVSFQLQPAHHPDFSTQTSAPPEDLSKHVCAALSQSMASKHVKHFHSSSPSASVPGSSSGTPCRGCRRPYGSSRTSEVGHMSVVTFSYIEKANIKKVESPHDLLIEEDDQPHKRFSDPAWTPSTCSNNSPLYQQAGSPALNRANVDSVGRAATQRALEDFGSPQLRSKFARGSDWSPGVRHNQQARCQSWSGTPVPDSSTHTNNNADRMEVSHGLSRSPTSNRLSSHTGHSNIPLDQNSSPKLQKGPNEHQQTQQVPSSPSQKNGGPLSGQKGSLVITNTHAEGLNQLSGSNTSSVLNSPEVARRLAEEATKVSVIFDEVRKSPSHNVFGGQPLYGPLHHQSPVMKMNIPVHGPHSESPITNGAPPCAQESTANQSTEARCALANHSHSPIPIGGPPDSPAIPSQIMHPWMSKMDVGSPLRDPRLHRSDSTSTESPTLHRRRPPQYIGWSHEEAWDLYPERCSEVRVDHTPSDSVEISRRLLIGQRIEEAPISWTSMQTWREQEEKEIEMFRSGEKQENEIEGSEQPGPMERQTEWRRRQALLLGPVVLEGEHEERGEVEQNGVPGSSPSSSGVTGSLGDRECLSPESSSNSNQTEHASSGIQSDGGPLVMGPSLHCQKIARAKWEFLFGTTTETTTKGREKNVLDPTTAPPSGHSTPTPPNSLPLEELALRANHDVHHVEVELVTPPPAAPGTSPKTGIIRRTLKYSETDLDAVPLRCYRETDIDEVLLADKDDSDSAFGSNRSVMGTSGTGSSPLGGTPYERTDGEEEEEDGAKEEEELEEDEEEDVASWASVRTQGDLRRQLTSQEQEEEEILSMLLKRPVDHFYKFHPALKSPVLVRGPRRHSGDNLDTFSRHFESIMESHRAKGTSYNSLDSLDLLTSSTQTVLTFDLPTLTPQVKGQMYQSARQIVELSFAPLAHVELPSVSESALTITGDSDATRAPSSERLSSGSDDRSGGHSWTSNPVYILPREKSSRLATDVDLDQELSERLGLGSSDTLTNGNRADLEAAKRLAKRLFNLDGFKKCDVARHLSKNNDFSRMVAEEYLGYFNFTGQTLDQALRTFLLEFALMGETQERERVLAHFSRRYLQCNPLLMLSEDSIHTLTCALMLLNTDLHGQNVGKRMSCTQFTGNLEGLNSGHDFPKELLKALYNSIKNEKLQWTIDEEELRKSFSELGEQRGDSNSRGMKRTGSGMVPPSGALLYKTGFLVRKVHADCDGKRTPRGKRGWKTFYAVMKGLILYLQKGEYRPDKQLSNEDLKNAVSIHHSLAIRATDYHKRPNVFYLRTADWRVYLFQAPNAEQMQSWITRINTVAAMFSAPPLPAAIGSQKKFSRPLLPGSTSKLSQEEQVQAHEARFHATSTELAELRSYPPDRKVKGRELEEYRLREEYLEFEKTRYETYSMLLRAKQRCGDDNLSVFEAMLLEEGSLQRAQSSPTLQESSLTCSTRDGPSTARDPSATGNGTGTGCVRGQGQRHSYRQAVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EIG1</accession>
    <name>A0A3B4EIG1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PSMG2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms a heterodimer with PSMG1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037391163.1">
        <property type="nucleotide sequence ID" value="XM_037535266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EIG1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028685.2">
        <property type="protein sequence ID" value="ENSPNAP00000035538.2"/>
        <property type="gene ID" value="ENSPNAG00000025672.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438869"/>
    <dbReference type="GeneTree" id="ENSGT00390000018415"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035538.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="255" mass="27853" checksum="B3CB02034E5CD14D" modified="2024-11-27" version="2">MFVSAEDSTPSFKGFTLILPAVSVGNVGQLAVDLIISTLNMPKVGHFHTDCLIPMVGNGPYSSLEQLSTNAEVYSHSDLKLAALQIRSPVLQNKGKLFRKLLVSWIKSSGFSRTVLLSSSHAYQRDDQQLKGPALRYLLSPALQDVGDTLRELGWAELEKVSVFPGINDSDKRHYIPGGGVTKGLYADCCAESVPLAVVLLFCSEGDNVPDAFTLTNHLNYWLHLLEKSPQGATPWKVPSSWRLLFGSGIPPLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EIJ8</accession>
    <name>A0A3B4EIJ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BAG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">BAG2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017549610.1">
        <property type="nucleotide sequence ID" value="XM_017694121.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EIJ8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035026"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027456.2">
        <property type="protein sequence ID" value="ENSPNAP00000035026.1"/>
        <property type="gene ID" value="ENSPNAG00000024676.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425463"/>
    <dbReference type="CTD" id="9532"/>
    <dbReference type="GeneTree" id="ENSGT00390000017590"/>
    <dbReference type="OMA" id="LHATKMI"/>
    <dbReference type="OrthoDB" id="6284251at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037689">
        <property type="entry name" value="BAG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12334">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12334:SF6">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="2">
        <location>
            <begin position="112"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="208" mass="23596" checksum="359669A0F394F233" modified="2018-12-05" version="1">MAQAKISAKMNDAKSAKFSRTISMADRSTRLLESLDEVEMRVEALREAATVMEQERESLLEMIQLIQNSQEMRTICDGEREELTLTANRLMNRTLTVQVTVDTIRNEQQEDALRRATAVIDEIAAKVLDDMEGARKRLQALHAACVTEAPPVPIDQKFQTIVISCALEDQKKIKRRLETLIRNMGNAERTIKIMDHQKVDESKNANGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJV0</accession>
    <name>A0A3B4EJV0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">trypsin</fullName>
            <ecNumber evidence="8">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJV0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035481"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039241.2">
        <property type="protein sequence ID" value="ENSPNAP00000035481.2"/>
        <property type="gene ID" value="ENSPNAG00000025514.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160085"/>
    <dbReference type="OMA" id="WIDSHIN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF54">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035481.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="96" mass="10158" checksum="FB47A9D3542E780F" modified="2024-11-27" version="2">LPTVLQKAEVSLMSQSECKRAYGPVSPRMLCAGVPSGEQDACRGDSGGPLSCQVSGGRWFLTGIVSWGSGCGRPNLPGVYTKVAKFISWIDSHIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK49</accession>
    <name>A0A3B4EK49_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EH domain binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">EHBP1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017560467.1">
        <property type="nucleotide sequence ID" value="XM_017704978.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK49"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035586"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028917.2">
        <property type="protein sequence ID" value="ENSPNAP00000035586.1"/>
        <property type="gene ID" value="ENSPNAG00000025894.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431671"/>
    <dbReference type="CTD" id="23301"/>
    <dbReference type="GeneTree" id="ENSGT00940000157597"/>
    <dbReference type="OMA" id="QQSSCDN"/>
    <dbReference type="OrthoDB" id="5972258at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd21254">
        <property type="entry name" value="CH_EHBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000023">
        <property type="entry name" value="EH domain-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF43">
        <property type="entry name" value="EH DOMAIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51848">
        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="454"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="BMERB" evidence="9">
        <location>
            <begin position="1099"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="251"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="642"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="832"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="907"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="982"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1071"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1239"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="642"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="803"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="838"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="914"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="992"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1071"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1248"/>
            <end position="1270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1270" mass="141682" checksum="2149D58175D6B9EE" modified="2018-12-05" version="1">MASVWKRLQRVGKHASKFQFVASYQELMVECTKKWQPDKLVVVWTRRSRRKSSKSHSWQPGIKNPYRGVVVWPVPENVEITVTLFKDPHAEEFEDKDWTFVIENESPSGRRKALATSSINMKQYASPMPTQTDVKLKFKPLSKKVVAAALQFSLSCIFLREGKATDEDMQSLASLMSMKQADIGNLDDFEEDNEEDEENRVNQEEKAAKITEIVRQLNALSSVDGDEDDPKLKPACSPAELISKLNFLEEDEQVQTHSSSNPFEEADDPLALNPFSDPDEEEVQPSLQSTLKDEESSNPFNPFDEPELEQDQEPQYKNPFDEPDPEPQVESTPPRGSARKMVRPVDMSKYLYADNSKTEEEELDESNPFYEPKSSSPALSTTEGSTEKTMKRRAPLPPAVTSVTPASPSVTPKPAVSEKTPSGPPAFPALAGRELASSSPKPSPVPSPILSGRPNASQSLLVWCREVTKNYRGVKITNFTTSWRNGLAFCALLHHFRPDLIDYKALNPQDIKENNKKAYDGFASLGISRLLEPADMVLLAIPDKLTVMTYLYQIRAHFSGEELNVVQIEANSGRSTYKVGDFETDTNSSIDQDKFYAELNSDLHRNESLKEPDGMAANGEGVEPEVASVTGASLGMSLSEAGLTSATSGPASVVTTTVTDSYPKPAPRTKISPDSSSTRPVALETEKAGATDMADSAGLMEREKERDGAPPAKVIDIQPKRPGSLRSLKADSGGLEPGTSPPHTQKLGFSYSRDADLSKKKRSSIRNAEAETKADSSPLQTNHRDAPVKHMQNVAPVPASTTNEEKKVQSRQEELKERARLLLEQARRDAALKAKNKTFPNATSPTRPQQIIDERDEERRRQLRERARQLIAEARSGVKMAEFPLASDASTTAAEFKGRSKAAGDVVDMVDGICVDDEEEGRADDEEQVAASLDDDDDVTGGQNEEEPVASCLMEEDFTNSATDLASLGVMNSRHVDLKLKKLTDVRPQGGSSPSSSSSSSSLTASTTSPLSPGEGPELGIQNNADELKAEHLRRATERLKNPVVFSKESAVRKTQLKSFSQYVETRPEVRRQRSVPEELKRVSEDRGGFSDMEGRRPTEDEQNVFRDTSQYVVGELAALESEQRQIDSRAARVEKRLRYLMDTGKNKDEEEAMMQEWFTLVNKKNALIRRQNQLSLLEREHDLERRFELLNRELRAMLAIEDWQKTEAQKRREQLLLDELVILVNKRDALVRDLDAQEKEAEEEDEHLERTLEQNKGKMAKKEEKCVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EMB9</accession>
    <name>A0A3B4EMB9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COX assembly mitochondrial protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CMC1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the CMC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559426.1">
        <property type="nucleotide sequence ID" value="XM_017703937.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EMB9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036923"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032214.2">
        <property type="protein sequence ID" value="ENSPNAP00000036923.1"/>
        <property type="gene ID" value="ENSPNAG00000028155.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431052"/>
    <dbReference type="CTD" id="152100"/>
    <dbReference type="GeneTree" id="ENSGT00940000166989"/>
    <dbReference type="OMA" id="CCQETGF"/>
    <dbReference type="OrthoDB" id="6224010at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013892">
        <property type="entry name" value="Cyt_c_biogenesis_Cmc1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22977">
        <property type="entry name" value="COX ASSEMBLY MITOCHONDRIAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22977:SF5">
        <property type="entry name" value="COX ASSEMBLY MITOCHONDRIAL PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08583">
        <property type="entry name" value="Cmc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="107" mass="12738" checksum="FDBD99FA4F44206A" modified="2018-12-05" version="1">MDTPNTEEPHLRHVELDVLIPKKMREKARELCSQHVQAFSTCCKESGFLMVLKCREENAALKDCLTKHYKDPEFFEECKQEYMREKLEYERTGIPTKKRKEKLPTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQH9</accession>
    <name>A0A3B4EQH9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017563937.1">
        <property type="nucleotide sequence ID" value="XM_017708448.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EQH9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037391"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040829.2">
        <property type="protein sequence ID" value="ENSPNAP00000037391.1"/>
        <property type="gene ID" value="ENSPNAG00000004523.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433684"/>
    <dbReference type="CTD" id="569156"/>
    <dbReference type="GeneTree" id="ENSGT00940000155960"/>
    <dbReference type="OrthoDB" id="10030361at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14229">
        <property type="entry name" value="STKc_HIPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000029">
        <property type="entry name" value="Homeodomain-interacting protein kinase 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000022">
        <property type="entry name" value="Homeodomain-interacting protein kinase 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF45">
        <property type="entry name" value="HOMEODOMAIN-INTERACTING PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="241"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="84"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="890"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="944"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1081"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="893"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="944"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1005"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="1088"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1262" mass="137806" checksum="73E3980FE8B1588F" modified="2018-12-05" version="1">MASQVLVYPPHVYQTQTSAFCSVKKLKVEPNNCVYHDRAFPLTFLNGRTLGIAQPTYKTRDFAAGQHRWQNFPVQTVAVRGVQRQKTSAAAPHKERGVLLQGKEPEDPEKGRKSSSGASGAAGGVRGEDSGSRGGGGGEGGGGADERKGEDTCNFNLADSSQRCGLKRKSEELENVGSAMQIVEDLSMLPAMLQANVGNPPVAVPAAVGGGPVKQGGGTGNGDGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTSEVVAVKILKNHPSYARQGQIEVGILARLSGENADEHNLVRAFECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIRPILQQVATALKKLKAMGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGEHLLNVGTKTSRFFCRESDSPYAAWRLKSTEEHETETGMKSKEARKYIFSCLDDIAHVNLVMNLEGSDLLAEKVDRGEFVVLLKKMLLIDAEKRIVPSDALNHPFVTMQHLLDFPQSNHVKSCFNIMDVCRSRPGTYDTINRNKAPFVRPVTTSAPNLTVPFSKMAGVHPQPLAPSAPSVMHPGIPLQTGAQFGDSFQQALILCPPTIQGLPPNPNKPAGYSVRMENTVPLVTQAPAIQPLQIRPGVITQQAWSNRAQQILVPAWQQVTPVAPPATTLASDSVAGPQRLADWGKVRPHGNHYSSIIPHPQPLLTNQMTMSAHQPISIGIAHVVWPQPAANKRNKPCGNRSNFLHNTNTQNSTCQSPKMANLGKNPEEEEEVEDHCPEAECVVEAESQAVEEEEPMCCKVEPEPNELGVSQKQRQAIVIADTPSPSSSVISISSDTDEEDEQRAQRHSLECKGSQDCEACQSTLNMERVCSLSSPDSSLSTSSSTSLQSSASPCKRPNSMSDDEQESGCDTVDGSPASDSSGRADSPFLESRYIADRNENCKPRVTTETESGKPPIRTMVVPPMRVQNNNEPLARTVLESSCLSKGRSGHNRQFHHSVSLLNRQPKITPASQAQHPPQPQQPPPTFGQVQHYNSSHQEWNGNYSHRRPQAFIPPTIHGHTFTLPHGSPTHTTAHPPPPHLGVHPHAHAQPALLSYPASAPLVTATPMAHLLASPGATRTHVLQPAYSLSHPAGHMVHPVAVGLNPRLLPSPTLHPQGQFKPLFPPHSYIASPAYAGFPLSPTKLSQYPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQW7</accession>
    <name>A0A3B4EQW7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017550255.1">
        <property type="nucleotide sequence ID" value="XM_017694766.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EQW7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037541"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033995.2">
        <property type="protein sequence ID" value="ENSPNAP00000037541.1"/>
        <property type="gene ID" value="ENSPNAG00000005438.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425807"/>
    <dbReference type="CTD" id="30170"/>
    <dbReference type="GeneTree" id="ENSGT01000000214804"/>
    <dbReference type="OMA" id="AENHCHQ"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793:SF12">
        <property type="entry name" value="TETRAPEPTIDE REPEAT HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="124"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="126"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="196" mass="22236" checksum="4AC94DA2181AF5EF" modified="2018-12-05" version="1">MDASKFSNFTIDYILGENSHTPHTQPVAESPGAAQVFRGDLSEANLLPSSGFNSQTRGGAVMGLQHPCPGIYDLTPYYTFNCCGPVTYYQANINTTCCGSERWFHPHPDCDREEAHCYQNGQQRQRNRIRTVFTENQTKQLDQLFSITEYPSVEARAELARNTGLSEETVRVWFKNRRARRKRQKPSSDPPQVDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ERA1</accession>
    <name>A0A3B4ERA1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein FAM47E</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546557.2">
        <property type="nucleotide sequence ID" value="XM_017691068.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ERA1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034420.2">
        <property type="protein sequence ID" value="ENSPNAP00000037681.2"/>
        <property type="gene ID" value="ENSPNAG00000029640.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423635"/>
    <dbReference type="CTD" id="100129583"/>
    <dbReference type="GeneTree" id="ENSGT00940000165114"/>
    <dbReference type="OMA" id="RSGCFTK"/>
    <dbReference type="OrthoDB" id="6755972at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032743">
        <property type="entry name" value="FAM47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46449:SF5">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 47 MEMBER E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46449">
        <property type="entry name" value="ZGC:158260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14642">
        <property type="entry name" value="FAM47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037681.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="48506" checksum="B8E4847B55B15FE6" modified="2024-11-27" version="2">MTERKRPIGLGEGAPLHPWYKERLRTKCLKDPVRKQQLSGALDGRGWRFLHPGQDDFRDGYPALRGEPLTQPQRRTGPPVLGLVNRDRLVTSPKKRFTKEQACFSKLNCLGHARRQYVEAVEQKLSAHPLALYPHLTSGLQPELINEILSVLDPEIHVKRESDHSCFGNEEECSEETGRKCEGPTQKPSKEKTTKTSAGTKPLMEGVKDSDSRNPYKWQETKETHAKEDQMVSDKFLHSPSQDEDIKKVTKLFCDWVTSLGGETNSLTESTILSLFFNGYEKKPALMFPIQVLGPNQIPEELHNTVEYATCKDYSHSAQLKVCTPNVKTTYGTWYLDSKTWKKQPANKPLRDPSVSKDLEQGQQLTEKDEQLKQIHGTQAFRQFIISKGLRMPRFISSLFLEEEQENRTTGTDTIASDSVHKGTAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJL8</accession>
    <name>A0AAR2IJL8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin related protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ACTR3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the actin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJL8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042428.1">
        <property type="protein sequence ID" value="ENSPNAP00000038207.1"/>
        <property type="gene ID" value="ENSPNAG00000024434.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155065"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000315">
        <property type="entry name" value="Actin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000803">
        <property type="entry name" value="Actin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000006">
        <property type="entry name" value="Actin-related protein 3 (ARP3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000002">
        <property type="entry name" value="actin-related protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="49580" checksum="17CBA405A3E5A749" modified="2024-11-27" version="1">MLCNLTVYTKLGYAGNTEPQFIIPSCIAIKESAKVGDQAQRRMMKGVDDLDFFIGDEAIDKPTYATKVSIQHTPTFLYFLSHNFSLYFCLKWPIRHGIVEDWDLMERFMEQVIFKYLRAEPEDHYFLLTEPPLNTPENREYTAEIMFESFNVPGLYIAVQAVLALAASWTSRQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFTQQLLREREVGIPPEQSLETAKAVKERFSYVCPDLVKEFSKYDTDGTKWIKQYTGINAISKKEFTIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGKLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJR4</accession>
    <name>A0AAR2IJR4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Perforin-1-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017578814.2">
        <property type="nucleotide sequence ID" value="XM_017723325.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391177.1">
        <property type="nucleotide sequence ID" value="XM_037535280.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037391178.1">
        <property type="nucleotide sequence ID" value="XM_037535281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJR4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062669.1">
        <property type="protein sequence ID" value="ENSPNAP00000039868.1"/>
        <property type="gene ID" value="ENSPNAG00000034381.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108442979"/>
    <dbReference type="GeneTree" id="ENSGT00530000063725"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF5">
        <property type="entry name" value="PERFORIN 1.2 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043568773" description="Perforin-1-like" evidence="8">
        <location>
            <begin position="24"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="10">
        <location>
            <begin position="31"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="391"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="64656" checksum="40897C8C9068170F" modified="2024-11-27" version="1">MGWMCRYLVSCWIWCSLLSMGNAEEQLELGSPKNCRNALFVPGHNLGGEGFDIVKMERKKSYIIDMEKWNIGNGSCKMSRNAYMNNARQKIPVAVVHWRNSAKCSMKVSSQVYDSSEALVKESTSSISNNWKNGLDLGFTGSMMVGGTYSREARFSMKKSKEDKFSFTKHAVECNFYSYKIKSSPPLHREFLRAVRSLPPNYNPHAYQHIIDMYGTHYTEGVVLGGKMKAVTAIRSCKAAMNGLSDTAVKDCLDVEASGTYKSATLSAEARECQRLKRTIGRDGKFSSMFNERQTEIVGGNINGEDLLFSRASHPYALHRWLNSLKTIPDVVTYTLRPLHSLLNNRHPARKGLKKAVEKYILQNALRKVCSRSCKIGHRNNPRDRCACICHASQNIRSNCCPSGTGLATLKVFNLRAKGLYGDFWTKTDGFVLVKYGHTVKRTAVITNNDNPHWRESFEYGPIKLSNSKTINFEVYDEDTYWNTDFLGKCSFPLTSGYVSKACYFRYGTFFFSYIAKCAPSLQGPKCADYSPSPMATPLAKIFHSRNGILVKDMWKLHTAQNYSDTVYFEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IKR5</accession>
    <name>A0AAR2IKR5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IKR5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067775.1">
        <property type="protein sequence ID" value="ENSPNAP00000040258.1"/>
        <property type="gene ID" value="ENSPNAG00000012007.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156097"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000008">
        <property type="entry name" value="Ubiquitin associated and SH3 domain containing B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000052">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF27">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="245"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="646" mass="72226" checksum="C37E8AA200EBF652" modified="2024-11-27" version="1">SDSGGQPNPMAAKDELYAKVTPRRQRQSRPGTVKHGSSLDVLLSMGFPKTRFSSFSLSVFFSRLFSHVDDPFLDDPLPREYVLYLRPSGPLLHQLSHFWQQSRAMCGKNKAHNIFPHITLCQFFMCADAKVEALCESLQTVVGQWRGRFPSPLPLELYTSSNFIGLFVEEKVAEVLKGFAADFSAEAAAKADVHVEPHKKQLHVTLAYHFPTNHLSSLEKLARGVDVSLGCDWLAVLFSRDIRFANHETLRVMYPYAPQNEDELELAPNDFIFMSSMEQGSASEGWVYGTSMSTGLSGLLPENYVSRADECDTWVLHVSHSFLNCTSPTNSGGAMGGSFLDGQLDGRLLDDPSLMDPASLNVICHPMQMLRTNSQSLLPKRALFVCRHGERMDVVFGKHWITQCFDAKGRYMRTNLNMPCSLPARSGGYREYDKDCPITVFGATQARLVGEALFDSHTVIDFVYCSPSLRCVQTAHNILRGLQQDGKTKIRVEPGLFEWTKWVSGTSLPAWIPPTELAAANFSVDTTYRPHIPISKLAMSESYDTYISRSFQVTREILTECKSLGNTVLIVAHASSLEACTRQMQGLSPQNSKDFVQVVRKIPYLGFCACEEMGETGVWQLVDPPILPLTHGPNHSFNWRETLLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IPG3</accession>
    <name>A0AAR2IPG3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037398319.1">
        <property type="nucleotide sequence ID" value="XM_037542422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IPG3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073173.1">
        <property type="protein sequence ID" value="ENSPNAP00000039561.1"/>
        <property type="gene ID" value="ENSPNAG00000031735.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108410946"/>
    <dbReference type="GeneTree" id="ENSGT01150000287002"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000005">
        <property type="entry name" value="MHC class I antigen ZKA transcript variant 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF193">
        <property type="entry name" value="LOC571647 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043714523" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="211"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="402" mass="44782" checksum="58B61AA18D114770" modified="2024-11-27" version="1">MGVSALVCAVLLCGGLVCALGDKHSLYYIYTALSKDVALPGIYEFTALGLLDDREIDYYNSQEQVKVPKQDWMKEKLQPDYWDKGTQSRKSKEQWFKVNVEILMQRMRHNETNLHILQWRHGCEIEEADGNLTFLRGIDEYSYDGDEFLSFDDENMRWIASVAQALPTKRKWDDVAILNQYTKGYLEKECVDWLSKFMQYGKESLRNHSKPEVHAFAKKSTTNSDKLTLTCLATGFYPKDVTLRVRKFRTSLPEHLLTSSGVRPNDDGTYQLRKSVEIQEDETAEYDCYVNHSTLEEPIIKQWDKMCFDCQSSGSGAVGPVIGGVVGLLLSLLSSGGIIFALIKKGIIANPCSGNATPALQAPPNGNGPVMTNGAANGHAEEVSLVPNGNGHIHENGTPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0T4</accession>
    <name>A0AAR2J0T4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NTM</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the immunoglobulin superfamily. IgLON family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0T4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044197.1">
        <property type="protein sequence ID" value="ENSPNAP00000043869.1"/>
        <property type="gene ID" value="ENSPNAG00000036407.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166369"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000305">
        <property type="entry name" value="neurotrimin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050876">
        <property type="entry name" value="IgLON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757">
        <property type="entry name" value="IGLON FAMILY OF IMMUNOGLOBULIN SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757:SF41">
        <property type="entry name" value="NEUROTRIMIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043400586" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="134"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="219"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="379" mass="41576" checksum="12EA81A9D11DA256" modified="2024-11-27" version="1">MEVYGCWKRLLPLCLRLLFLVPAGLPVRSQGDSQSDNKVMDNITVRQGETVFLSCSQGDVVTHTAWLNRSSILYAGEDKWSVDPRVSLVTLNRDEFTIKIENVDMSDEGLYVCAVQTSSKPRTTSVHIIVQVPPKIVNFSKDLVVNEGANVTLMCLASGKPEPAISWKMLSPSGDGLGSDDEYLEIPSITRQRAGEYECSAVNDIATETKTMQLVVNYPPTVFEGRDVGVTLGQRGVLQCEADAVPEADFEWYRDDRRISNGFDGIEIEDSGALSKLTFFNVSETDYGNYTCVAINKLGSANTSFVLYEVIEPTSSTLLQVDTASLLEVTSSVLEQTATEYNQFWEGPGAVQDGNSGTSGTYTPICLLLILALQLLLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J176</accession>
    <name>A0AAR2J176_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J176"/>
    <dbReference type="Ensembl" id="ENSPNAT00000042140.1">
        <property type="protein sequence ID" value="ENSPNAP00000045725.1"/>
        <property type="gene ID" value="ENSPNAG00000013715.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164944"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01873">
        <property type="entry name" value="RhoBTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000177">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000014">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043591170" description="BTB domain-containing protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="520"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="324"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="324"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="738" mass="84004" checksum="751ADC3E953D07CD" modified="2024-11-27" version="1">MMILHVSLSVFLSLHRALSMDIDTDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSLANPNSLRHVRTMWYPEIKHFCPRTPIILVGCQLDLRYADLDAVNRARRPLAKPIKPTDILPPERGHEVAKELGVPYYETSIVAQFGVKDVFDNAIRAALISRRHLQFWKSHLKKVQRPLLQAPFLPPRPPRPVVGIPDPPPMDGEGPDSLFCQPLCADVLFLLQGGATRVFAHKVYLATSCSKFYDLFTMDLGDAEEDKDGEDTLEGSEEEEQSRIGAKEQAGRTKSLDIDKDGEGGTRGPKRPLLLEQGSLRTSQSDNALPARSQCTMGPLGAGRALLGWGRGFLGVYLERVDDPVTGRPRLMTVVSMDELIQEASFRAVLQYLYTGHLDEGRSDLMQVATIAELLEVFDLRMMVANVLNRESFMNQEITKAFHVRRANRIKECLCKGTFADVVFQLDDGYLPAHKPLLISSCDWMAAMFRGSFMESYIEEVSIPNTSTACMRAVLEYLYCGLLTPCPELEPMDLIILANRLCLPRLVALTEQHAVEELLQWAVKGADIDGQVLAYLEMAQFHNAKQLSAWCLHHICTNYNSICRKFPKDMKAMSPENQKHFEKQRWPPVWFLKEEDRYNRSKKEREREEEILRKQHIKRGWCFWRHPSHPKSLAPSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JC44</accession>
    <name>A0AAR2JC44_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JC44"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062270.1">
        <property type="protein sequence ID" value="ENSPNAP00000047902.1"/>
        <property type="gene ID" value="ENSPNAG00000030914.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161131"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF9">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="207"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="299" mass="33175" checksum="478578FF0FF7EFB4" modified="2024-11-27" version="1">GQMIVKDLQPLSIVEDQGFQAFGELSLIKQTVDALRPFEEVTREVSADKYVSVSKVIPLVSLIHRAVAACECQGSSLAAQLAQQCQRRFRGIESIYCLAASTFLDVRFKHLGFRDKDNVEVMKKRLLSEMQEVYQTRQAAPTLAPSSTATVSSSPYTSTSGSSTSEPNQSPAPSGSAVSKGGIWTDFDMQVVSGQHHRSAASDVLIEIRRYSEEKVIPRDSDALVWWKEHEPTFPALSKLAVRHMGITATSVPAERVFSKAGEVLSKKRNRLKGKTVNMILFLNKNLLLPQYTLFKRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JDL0</accession>
    <name>A0AAR2JDL0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017558221.1">
        <property type="nucleotide sequence ID" value="XM_017702732.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JDL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075846.1">
        <property type="protein sequence ID" value="ENSPNAP00000048314.1"/>
        <property type="gene ID" value="ENSPNAG00000035398.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430313"/>
    <dbReference type="GeneTree" id="ENSGT00990000204366"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF34">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043871127" evidence="2">
        <location>
            <begin position="24"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="271" mass="29858" checksum="6425BA9114325018" modified="2024-11-27" version="1">MGHPIAVLTFTMFLGQGMVVVLAQSSPSVSLGSVRKKSGCIKVNHCKCILGDGSGLIDLEPLADVQGFLEHSKHVPLEDSLQDTEVLLSFSPCLPFSQPEFTITDCTDVAACLILRSRSHTDRYVNYGRHEGNKFHYNETTKTLSVSYSVTPYSEAQTVVHYSCSPNRSISYNLRLTHSEAPLQIWVQSPCACPNACAVVDVGPGNIFLIILCLSITAYFILGSCALRPFRSSNGVQIAPEDSVWCMLCYKFTDQPSSKRKRHCSLREDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JES2</accession>
    <name>A0AAR2JES2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC9B2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JES2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083063.1">
        <property type="protein sequence ID" value="ENSPNAP00000050435.1"/>
        <property type="gene ID" value="ENSPNAG00000029014.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013285"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000012">
        <property type="entry name" value="sodium/hydrogen exchanger 9B2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051843">
        <property type="entry name" value="CPA1_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102:SF23">
        <property type="entry name" value="SI:DKEY-162B23.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="9">
        <location>
            <begin position="145"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="557" mass="59367" checksum="B75FF0049A903779" modified="2024-11-27" version="1">MDDAQSRPHSSRSVCSQRTEDECPVVGNHDEHHLSVNQTLQVLARRPDSPSTTVTEETVFIPRAVHSVDACTNTDPPHKCCGWLRRQCPCPPRGLLAYIITKVSMAALLFGVVWSITEKECLPGGNLFGIVTVFICALTGGKLVGLIHIPNLPPIPPLLGMLLAGVLLRNIPVVTEAVYIDFRWSASLRNIALAVILARAGLGLDGSALKKLKAVCVRVAMGPCLIEASTVALVSHFLMGLPWIWGFILGFVLGAVSPAVVVPSMLLLQKDGYGLEQGVPTLLMAAGSFDDILAITGFTTCLGIAFATGSTWFNILRGGLEVLGGMVAGLAFGFFLHYFPSKDQKDLAVKRSFLLLGLSVFAVFGSNVVGIPGSGGLCTLVLSFVAGVGWRKAKAPVEEIVGKAWDVFQPLLFGLIGAEITISALDPRTVGLGLAALCIALTVRIFFTFLMVLCAGFNFKEKLFIALAWMPKATVQAAIGSTALDMARSKQEKQMEQYGMDVLTVAVLSILLTAPIGALLIGLCGPRLLQRPKNPEWAMSQGALSASETPVTYESAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JK83</accession>
    <name>A0AAR2JK83_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Neutral ceramidase</fullName>
            <ecNumber evidence="2 6">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the neutral ceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="2 6"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JK83"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058428.1">
        <property type="protein sequence ID" value="ENSPNAP00000052443.1"/>
        <property type="gene ID" value="ENSPNAG00000007696.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015792"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2300:FF:000001">
        <property type="entry name" value="N-acylsphingosine amidohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2300">
        <property type="entry name" value="Neutral/alkaline non-lysosomal ceramidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038445">
        <property type="entry name" value="NCDase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031331">
        <property type="entry name" value="NEUT/ALK_ceramidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670:SF1">
        <property type="entry name" value="NEUTRAL CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04734">
        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17048">
        <property type="entry name" value="Ceramidse_alk_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="7">
        <location>
            <begin position="60"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="7">
        <location>
            <begin position="313"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase C-terminal" evidence="8">
        <location>
            <begin position="511"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="683" mass="75460" checksum="286FFB96FAE8137B" modified="2024-11-27" version="1">MARKAVCCGLSSLEALLMVLFLLMTGISVGLVTALAINLNSQTQETKEPIPSAKPLDNPFLIGVGRADCTGPVGDIPMMGYANLDQTAGGIHTRLFSRAFIVDDGSRRVVFVSAEIGMVSQRLRLEVLKELEVKYGDLYRQDNVILSGTHTHSGLAGYFQYTLFMITSKGYLKSSTQPIVRGIVKSIDVAHNNMKPGRIFLNKGEIVDSSLNRSPHSYMNNPKEERERYKSNTDEQIVTLKFTDLDGDGMGIFSWFAVHPVSMNYTNRLVSSDNLGYASYLFEQEKNIGSLPGEVRLKCVLCAIHSCFHLYLYKAEQELHGSIHAAHQWVNMTDVTVQLNSTHNVKTCKPALGHSFAAGTIDGGGDLNFTQGAVEGDPFWDGIRDSVLGTPSKDIQDCHDPKPILFSTGEMNRPLPWHPAIVDVQIITIGSVAIVAVPGEATTMSGRRIREAVKQELEMHNSFTNTEVVITGLCNIYTHYITTFEEYQVQRYEGASTIYGPHTLSAYIQHFRGLAKAIVEGKVGELPKGPEPPFFTNVFPLLGEPPVDKVPENTTFGQVLEQVDSLYKVGEVVSVTFVAGNPRNSGDMRGKTFITVERFHNSSGTWEVIHTDASWETRFHWIKGSGGQSNATVEWHIPLSAAAGTYRIRHFGHYKKRVVITPVITAYEGASDVFQVSKTLYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLS0</accession>
    <name>A0AAR2JLS0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Furry homolog b (Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSPNAT00000058381.1">
        <property type="protein sequence ID" value="ENSPNAP00000051139.1"/>
        <property type="gene ID" value="ENSPNAG00000016552.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00610000086058"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045842">
        <property type="entry name" value="Fry_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029473">
        <property type="entry name" value="MOR2-PAG1_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF29">
        <property type="entry name" value="PROTEIN FURRY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19421">
        <property type="entry name" value="Fry_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14228">
        <property type="entry name" value="MOR2-PAG1_mid"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="2">
        <location>
            <begin position="206"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1237"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1665"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1806"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="3">
        <location>
            <begin position="2075"/>
            <end position="2328"/>
        </location>
    </feature>
    <feature type="domain" description="Protein furry C-terminal" evidence="5">
        <location>
            <begin position="2354"/>
            <end position="3053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1594"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1987"/>
            <end position="2028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2046"/>
            <end position="2068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2397"/>
            <end position="2423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2463"/>
            <end position="2486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2532"/>
            <end position="2620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2011"/>
            <end position="2020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2046"/>
            <end position="2059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2400"/>
            <end position="2410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2562"/>
            <end position="2574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2586"/>
            <end position="2599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="3053" mass="342295" checksum="5DA8150EEF16A0D1" modified="2024-11-27" version="1">MSKEDEGNTGDVVPSKPDSWDTQSQDSIVLVQIASLSMDMFTPGDFKGKFRKIQPKKRPTIIRDSTPASPVGKPPLPPVSIGQGERKGPVAIMPVTVDPESKPGEYVLKSLFANFTTLSERKIRIIMAEPLEKPLSKSLQRGEDHQFDQLISTMSSLAEYCLPSILRTLFDWYKRQNGLEDESHEYRPRANTKSKNDEQQKDYLMERRDLAIDFIFSLVLIEVLKQIPLHPLLDGLIQEVINLAFKHFKYKEGYLGPNTANMHIVADLYAEVVGVVAQSRFPAVRKKFISELKELRQKEQSPYVVQSIISLIMGVKFFRIKMYPVEDFEASFQFMQECAQYFLEVKDKDIKHSLAGLFVEILVPVAATVKNEVNVPCLRNFVESLYDTTLDLSSRKKHSLALYPLVTCLLCVSQKQVFLNRWHVFLNNCLSNLKNKDHKMARVALESLYRLLWVYMIRIKCESNTATQSRLTSIISTLFPKGSRSVVPRDMPLNIFVKIIQFIAQERLDFAMKEIICDLLSVGKSAKAFSLNPERMNIALRAFLVIADNLQQKDGEPPMPNTGATLPSGSTLKKKKTYLSKTLTEEEAKLIGMALYYSQVRKAIDNILRHLDKEVGRCMMLTNVQMLNKEPEDMITGERKPKIDLFRTCVAAIPRILPDGMSKPELIDLLARLTVHMDIELRLIAQNSLQSLLLDFSDWREDVLFGYTNFLLREVQDTQQGLQDTSVKLLLQLLTQWRLALQTPGKRSAELGSRLPVERSPHSTVLHAVEGLALLLLCSCQTSTRKLAVAVLREIRCLFTAIGQAEDDDKPMIEVMDQLSPIVLDSFVHVAVSDSATLPLSHHVDLQWLVEWNARLVNSHYDVKSPSHVWIFAQSVKDPWVLCLYSFLRQEHLPKHCPMALSYAWPYAFTRLQLLMPLVDPNSPVYAKKTSTPGPADGYVTLWRNYLILCLGVAKPSIMSPGHLRASTPEIMATTPDGSVTYDNKVIGTPSVAWLLKQLVPLMRLESIEMTESLVLGFGRTNSLVFRELVEELHPLMKEALERRPENKKRRERRDLLRLQLLRIFELLADSGVISDSTNGALERDSLALGALFLEYVDLTRMLLEAENDKELEILKDIRSHFSGMVANLIQCVPVHHRRFLFPQQSLRHHLFILFSQWAGPFSVMFTPLDRYSDRNHQITRYQYCALKAMSAVLCCGPVFDNVGLSTDGYLYKWLDNILGCHDLRVHRLGCEVVILLLELNPDQVNLFNWAVDRCFTGSYQLASGCFKAIATVCGSRNYPCDIVTLLNLVLFKSSDTSREIYEISMQLMQVLEAKLSVYSKRIVGQKAGNILYGTHGPLPPLYSVSLSQLSNQLACMYPELTLPLFSEVSQRFPTTHPNGRQIMLTYLLPWLNNIELVDTGLLPPASSPCTPEEETREQTDGIGLAHRLKGNGWGSLQATSLVLNNLMYMTAKYGDELPGPEMENAWNALVTNDKWSNNLRITLQFLISLCGVSSDTTLLPYIKKVVIYLCRNNTIQTMEELLFELQQTDPVNPVVLHCDNPPFYRFAASSKISAVASGTTSSSNTVVAGQENFQETDEAKIVRENEERLSNAARAHNRLESRYSNSSGGSYDEEKNDPLPPYAGWLLNVLESNRPQPLPMPVNGGCWAPLVDYLPETITPRGPLHRCNIAVIFMTEMVVDHSVREDWALHLPLLLHALFLGLDHYRPEVFEHSKRLLLHLLIALSCNNNFQVIASVLLLTREISDNKTLTVKSSFQPEYLHTGGLDFLREWQASPVADSGLSSSSTSSSVSLGGSVGNLPQITADLEDLDESSNESEEKTNKLIEFLTTRAFGPLWCHEDITPKNPNSKSADQLTNFLRHVVSVFRESKSDFHLEQQLSDVALQTALCSSSRHYAGRSFQIFRALRQPLSAHAVSDLLSRLVEVVGEHGDEVQGYVMEVLLTLESVVDNLAECLKNSDLMAALTRASSPDFLVSGKLNCNRKSTGQLNLLQEGGTGERSRHQRSYSVPKKFGESGERSSSDPPRSATLDRIQACTQQALARSAHSALSSSSTRADSSAVTDPSHASHPSNLLSTIFWVAVSLMESDFEFEYQMSLRLVHKLLARVPLEMPENRERLEKLQAQLKWSGFAGLQQLMLKGFTSPATSDLTLQLFCQLTPVSRVPVVDTSQAIGFPLNVLCLLPHLVQHFDSPTQFCKESAERIAQVCLEEKEKNTKLSHLAHVMTLYKTRSYTRDPFSWVSVVCRYLHEAFSDITLNMVTYMAELLDKGLPSMQQPLLQIIYSLLSHMDLAGIQVKPFNVEVLKTIEKFVQTVHWKEALNVLKLVVSRSASLSLPSYAHGDLANLEVSRMMWDGSSKALPGKTLDFHFDISETPVIGRRYDELQGSAGRDGKARAIAVTRSASSTSSGSSSNPVLVPVSWKRPQSSQKRTREKLVNVLSLFGQEVGLTKNPSVIFSSCGDLEMMERQPSQVSSEDGTREDTLDDTASEQQFRVFRDFDFLDVELEDGEGENLDSFNWGVRRRSLDSTDLTELLEESQHSDSTPSLGPHDPQHDSDDSSEEESNSTSQSLSHSQLTMNLSPSEDTNHTDSLSTSYDTTSADPNSLNAATPCLGTSLPDDTVIPPPLPEDEDSNVQEDDLSFCVSELPPGFDCGSSFTLNLPPEPERSEMDFDPNRLPGYSFGEDRDDLDDLGFPPPPSPFFSAILAAFQPTACDDAEEAWRTHINQLVCDTDGSCAVYTFRIFYSLFQNIQNKFCSLTTDAAGYLGDSLRGIGTKFVRSSQMLKTCSDCPTLFVDADTIMSFGLLEMMKFSVLELQEYLDTYINREEAAVSWLQNCKATFPRCPDDGVITCQPGDTEEKQMETLAQLELCQRLYKLHFQLLLLFQCYCKLIMKVGSISSVPELLNMSRELGELKVSLKLAATGAAGDQDLHQRPGAQPSFSTSEAAVQAVLECLRNSHYATAVRHIRQCRRLWPNDIFGGNSEDEVQTLLNIYFRHQSLGQTGTFALVGSSQDPSQICTKLMELNGEIRDMIRRAQGYRVVSSSLVDSSASAVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JR57</accession>
    <name>A0AAR2JR57_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JR57"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059472.1">
        <property type="protein sequence ID" value="ENSPNAP00000054498.1"/>
        <property type="gene ID" value="ENSPNAG00000029845.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165002"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002980">
        <property type="entry name" value="Na/ntran_symport_GABA_GAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF316">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 6 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01195">
        <property type="entry name" value="GAT1TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="633" mass="71185" checksum="0B8D1C46278E2760" modified="2024-11-27" version="1">MGKTGQSTVDLNSPTEVKQAVPFEELNHNMTLPAEEKKPDLPDRGTWKGKFDFLLSCIGYAIGLGNVWRFPYLCGKNGGGAFLIPYFTTLIFAGIPLFLLETALGQYTSVGGLGVWKLIPMMKGVGLAAAVLSFWLNIYYIIIIAWALYYLFSSFRSELPWQSCDNPWNTENCFSNYSLPDTTNLTSAVTEFWERNMHQLTNGLEEPGELRWPLAGTLALAWVLVYFSIWKGVEWTGKVVYFSATYPYFMLFILFFRGVTLPGAKDGVLFYITPDFKKLLKSEVWLDAATQIFFSYGLGLGSLIALGSYNPFNNNVYKDAVIVCCINSFTSMFAGIVIFSIVGFMSYITKKPIQELAASGPGLAFLAYPQAVTQLPMSSLWAILFFSMLLMLGLDSQFCTVEGFITALMDEYPTVLRKRKKVFILIVCIISFLIGLSNITQGGLYVFKLFDYYSASGMCLLFLVFFETISIAWFYGADRFYNNIEDMIGYQPCSWWKLCWLYFTPLILKLPLVAIEMTPLTLGKYVYPAWGQGIGWLMALSSMLLIPGYVIYMFFSTKGSIRQARLLLGPVLSICGQTQLINTQAFPFCAPSMLSYSLFPSLFGFILANSTAPALLPSTHLFHCELERVYKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JSQ8</accession>
    <name>A0AAR2JSQ8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Spartin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Lipophagy receptor that plays an important role in lipid droplet (LD) turnover in motor neurons. Localizes to LDs and interacts with components of the autophagy machinery, such as MAP1LC3A/C proteins to deliver LDs to autophagosomes for degradation via lipophagy. Lipid transfer protein required for lipid droplet degradation, including by lipophagy. Can bind and transfer all lipid species found in lipid droplets, from phospholipids to triglycerides and sterol esters but the direction of lipid transfer by spartin and its cargos are unknown. May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with ITCH and WWP1. Interacts (via MIT domain) with IST1; leading to the recruitment of SPART to midbodies. Interacts with MAP1LC3A and MAP1LC3C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037402986.1">
        <property type="nucleotide sequence ID" value="XM_037547089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JSQ8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083389.1">
        <property type="protein sequence ID" value="ENSPNAP00000053312.1"/>
        <property type="gene ID" value="ENSPNAG00000020145.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438974"/>
    <dbReference type="CTD" id="100005765"/>
    <dbReference type="GeneTree" id="ENSGT00390000012235"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061724">
        <property type="term" value="P:lipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000009">
        <property type="entry name" value="spartin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF43">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MIT" evidence="19">
        <location>
            <begin position="12"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="124"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="562"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="576"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000053312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="589" mass="63134" checksum="B5E761D5D84C043B" modified="2024-11-27" version="1">MEASEPAELQVIKSHYEKAFECLDAGLLEDKAGNKGRAVVLYRLGRRHLLQGLEVATRGEHCAGADWDCARQMQLKMNETLSSITTRLAVLETACQGASGAQPLYPSLPVLQQPQRPARRPILNSRLSPTASGGVPFLPSSPVTASDVPSELPPAYTPQPTDGHFSLSHSEGKDWPSLPRPKQISRHQSLNEGGEDLLFLPYGVQIFFVTADGQVSAPSYPGYLRIILNDRQRHRDSNDISDIRQPPAYLQVCDWHYPLFPDSPVLLSNTGVFTFPDTTAAVPGSYVGVVLSSELSATDRALFQEHLSVLTQLRVQAADDEAGEPTGTDVVNLNEKAPISPSSDTATAMDTSPGKEENILPEWSEKMSQSILAGASWLSRGLVQGAEVTGKAIHKGASKLREHITPEETPAEVSPRVTRGLYVAKQATGGAVKVSNFLVDGLSSVVELVGKELAPHVKKHGSKLIPESLKKNKDACTNMGGAKVVAASSLKGLSTLWSGLETAAKTIGKSASSETVLTVKHKYGEEAGQATDTAVQSVVDVGVTAFNMDNIAVKAVLKGAGKQTSKALAEEDETMEPEKAAKRKEQEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JT27</accession>
    <name>A0AAR2JT27_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017557120.1">
        <property type="nucleotide sequence ID" value="XM_017701631.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JT27"/>
    <dbReference type="Ensembl" id="ENSPNAT00000054137.1">
        <property type="protein sequence ID" value="ENSPNAP00000055203.1"/>
        <property type="gene ID" value="ENSPNAG00000036773.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429688"/>
    <dbReference type="CTD" id="768149"/>
    <dbReference type="GeneTree" id="ENSGT00530000063936"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09583">
        <property type="entry name" value="SAM_Atherin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048589">
        <property type="entry name" value="SAMD1-like_WH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF139">
        <property type="entry name" value="ATHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21524">
        <property type="entry name" value="SAMD1_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52014">
        <property type="entry name" value="SAMD1_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAMD1-like winged helix (WH)" evidence="7">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="317"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="83"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="42311" checksum="3D2A39108D99477A" modified="2024-11-27" version="1">MSEPRFREWILDTIDSLRSRKARPDLERICRMVRRRHGSEPDRTCVELEKLIQEHAVLKVNYKGSVSYRNAAKVQRGRRKLEQASALKPAAKRACTAEVNRDGGGDNALGPADEEPMEDGEDELSTTAGLDSSMDEEEASLDAPCMQSTADSGIGLSPITDAGASLPAASPSAPKSSPSHSSMEPERETPHTPPEHTQPAAPQDTGSPELELARQPAAICPSAQRSTLTGEEGPRVEESALTPDQLLPDCRASLHQDEAVGSDREPAKGSVPAEEGKKCSKGDDVACPQEIEQAKNGGIKRGVSVKPEEIAQDLLQWSVADVVSYFTAVGFPEQAVAFRTQEIDGKSLLLMQRSDVLTGLSIRLGPALKIYEHHVKVLQRSHFQEDGDVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JYC5</accession>
    <name>A0AAR2JYC5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JYC5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049899.1">
        <property type="protein sequence ID" value="ENSPNAP00000057063.1"/>
        <property type="gene ID" value="ENSPNAG00000011671.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234532"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000037">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000005">
        <property type="entry name" value="sodium/potassium/calcium exchanger 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF21">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="69"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="349"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="517" mass="57114" checksum="598194BBF64BEC13" modified="2024-11-27" version="1">MVVFPVFPGHGMIVDGGSQNHDHWGRRLMASMADNDTQEKNCTEPAVHEFPDDFFTNTERKSGAVLFHILAALYMFLALAIVCDDYFVTSLEKICEKLDLSEDVAGATFMAAGSSAPELFASVIGVFITHGDVGVGTIVGSAVFNILCIIGVCGIFAGQVVLLTWWAVFRDSVYYIVNLTWEISTKWIVPQTLMLCCGCVCITVYALPLVKPSKAFTRGSVVMVDEIINASPPNYRFPEAGLRVMVTNHFGPKTRLRMASRLIITERQKLVQGANGVETAVIDGKPDIENGNVPEDKPSEEEDGELTSPFRIPRGCMNKTKWLISWPLLLLLFFTIPNCAKPRWEKYFMFSFLLSTVWIAIFSYFMVWMVTIIGYTLGIPDVIMGITFLAAGTSVPDCIASLIVARQGLGDMAVSNTIGSNVFDILVGLGVPWALQTMAINYGSVVMINSRGLVYSVVLLLGSVALTILGIHINKWKLDFKLGVYVLILYAVFLCFSIMIEYNVFTFVNLPMCQEDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1C6</accession>
    <name>A0AAR2K1C6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Schwannomin interacting protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017558862.1">
        <property type="nucleotide sequence ID" value="XM_017703373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1C6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071155.1">
        <property type="protein sequence ID" value="ENSPNAP00000058050.1"/>
        <property type="gene ID" value="ENSPNAG00000030869.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430722"/>
    <dbReference type="GeneTree" id="ENSGT00390000011127"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035332">
        <property type="term" value="P:positive regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039045">
        <property type="entry name" value="SCHIP_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015649">
        <property type="entry name" value="SCHIP_1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13103">
        <property type="entry name" value="SCHWANNOMIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13103:SF4">
        <property type="entry name" value="SCHWANNOMIN-INTERACTING PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10148">
        <property type="entry name" value="SCHIP-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Schwannomin interacting protein 1 C-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="252" mass="28635" checksum="AE38F52ACED4AB3C" modified="2024-11-27" version="1">MVHQEKCLYQAQRNERESIRQKLALGSFYDDGPAFFTGCSKGGKNCLSSRLQNGVNLQMCFVNDSSSDKDSDAEDSRTETSLDTPLSPMSKQSSSLSDRDTGEEDLEQEDCEFWRVQRRLQEEARGALALARPMARMQVEVERQIQLHRRSPVADLLPHLPHISESLMKRSLRPVDLRDMSLGQLQVITNDLHSQIQGLNEELVQLLLLRDELHVEQDAMLVDVEDLTRHAHSQQRHMAEKALGKRKGACSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K559</accession>
    <name>A0AAR2K559_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556025.1">
        <property type="nucleotide sequence ID" value="XM_017700536.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K559"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041876.1">
        <property type="protein sequence ID" value="ENSPNAP00000057592.1"/>
        <property type="gene ID" value="ENSPNAG00000032482.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429061"/>
    <dbReference type="CTD" id="559124"/>
    <dbReference type="GeneTree" id="ENSGT00940000164486"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000030">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF94">
        <property type="entry name" value="PHOSPHATIDIC ACID PHOSPHATASE TYPE 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="149"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="325" mass="35878" checksum="86FB570A0DCCE2F2" modified="2024-11-27" version="1">MHKLNPTDGERDLEVQQPLAQSSLACEGMRENGKHTLGQSPSAAGAPCSQRKMLVGLDILCLFVASIPFFACELKAVKPYRRGFFCGDPSITYPYLENEAIPDSLLIAGGIIITGLTIAVGECYRVRFRGVQSRAFVRNCYVSCLYKELGAFLFGCCIGQSLTNMAKLSVGRLRPNFLSVCNVTYASLRCTPDTYVADVNCICTSKEEEEARKSFFSGHASFAMYTMLYLAFYLQARLSWRGARLLRPLLQFLLVMLAMYTGLSRISDYRHHPTDVLTGFLQGALTAYWVAFHISSMFKACTSNLSPTNLSLDSPLSVSSGQTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K796</accession>
    <name>A0AAR2K796_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017578374.2">
        <property type="nucleotide sequence ID" value="XM_017722885.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K796"/>
    <dbReference type="Ensembl" id="ENSPNAT00000084700.1">
        <property type="protein sequence ID" value="ENSPNAP00000060193.1"/>
        <property type="gene ID" value="ENSPNAG00000005403.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442713"/>
    <dbReference type="GeneTree" id="ENSGT00940000163961"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF358">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="26"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="345"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="505"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="30"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="195"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="560"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="583"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="742" mass="82539" checksum="9A9101B4711A4B4F" modified="2024-11-27" version="1">MSLGLSSLASLSMPGSSRVSVTAADSRESMRGLNTRLADYLAQVRALQEANSKLEMQIRERVSSRGSEERDWTEYDKTIAHLRNQLTELNMDNARLMLQIDNARLAADDFKVKFEAEHAMRQGVDQDVANLRKMIDDTHMSRMQLESEIESLKEELIFLNKNHEEDAANLKGQIRGSSVDVQMEARKGDDLSETIDKIRQQYEKTMQKSREETEAWYQAKFDSMTAEVSESTETIQQGKSEVNDLRRQRQMMEIDLQALRSMNHSLEDNLKDTAGRSAQEMNHHNATIQQLEAELGEVHAQVGRQGVEYQALLNIKIKLEEEIATYQHLLEGTGVDDSFRDTGSRSGGNIRGAGAGTRDDVKGTGTREDVKNKSTGTRDVNVKGTGARDDVNVKGPGAREDVTIKGTGTRDNVNIKGTGTRDNVNIKGTGTRDNVNIKGTGAQEDVNVKNNGVPEDPNSHIRDTTVPEDDLVEFSLEQALNAAPPVPTPDQIHIISEEIQEEEVVSHSELKLNAANGTVEEEEEERGRDGGEEEAVVPKEVPPSRSTVEDVWGYVGVKPAAEEKKDEAEQKKEEKPAAQVKPVVEEKKEDPEQKVEVKPAVVEKKEDPEQKVEVKPAVVEKKEDPEQKVEVKPAAEEKKEAAEQKVEVKPAAEEKKEAAEQKAEVKPAAEEKKEAAEQKAEVKPAAEEKKEAAEQKVEVKPAVEARKDDAEQAAEKKHEDVVRQDGEVLEVKPVLNEEEQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K8Z1</accession>
    <name>A0AAR2K8Z1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Centromere protein K</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CENPK</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-K/MCM22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017577297.1">
        <property type="nucleotide sequence ID" value="XM_017721808.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K8Z1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000071134.1">
        <property type="protein sequence ID" value="ENSPNAP00000058556.1"/>
        <property type="gene ID" value="ENSPNAG00000034677.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108441993"/>
    <dbReference type="CTD" id="64105"/>
    <dbReference type="GeneTree" id="ENSGT00390000006243"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020993">
        <property type="entry name" value="Centromere_CenpK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14401">
        <property type="entry name" value="CENTROMERE PROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14401:SF6">
        <property type="entry name" value="CENTROMERE PROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11802">
        <property type="entry name" value="CENP-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="31072" checksum="83DE829793B0188B" modified="2024-11-27" version="1">MEQQSKIPETPAETDTGCSEAMKAELLDECEEQFGLLQKLQNEIILADTDCNDEESNEAVNRLNAMTTELEQWQEMEPKLLSTNPEILFALGKDELQRLNSQLKMVLSCSQVKRDALKDLLQREQKWLGEKKEVLSEVTEQVATLRNENEKLSEHSILQDMKRKITRVKDYHGNLMETLSDMLAEHFPLPNQQAVGTKKKRGTVPDSGENLISLSEILEQLTNKTLETPHEPYVSLDDSFWPPYIEMLLRNGIAVRHPEDCNKIRLENFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KBP9</accession>
    <name>A0AAR2KBP9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Guanine nucleotide binding protein (G protein), alpha 13b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(12) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580807.1">
        <property type="nucleotide sequence ID" value="XM_017725318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KBP9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065366.1">
        <property type="protein sequence ID" value="ENSPNAP00000059877.1"/>
        <property type="gene ID" value="ENSPNAG00000035889.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108444258"/>
    <dbReference type="CTD" id="326825"/>
    <dbReference type="GeneTree" id="ENSGT00940000157054"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031752">
        <property type="term" value="F:D5 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000004">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000754">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000469">
        <property type="entry name" value="Gprotein_alpha_12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF85">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT ALPHA-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00440">
        <property type="entry name" value="GPROTEINA12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="57"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="196"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="221"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="290"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000059877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="43793" checksum="8226D2AC8638C8DC" modified="2024-11-27" version="1">MADFLPSRTALVCIPACFLSSNEVEQQRKSKEIDRCISREKTFVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQRAKEEFRATIYSNVIKGVRVLVDAREKLHIPWGDPSNQAHGEVMMGFDTRSSTMAQGIVETKIFLHYLPSIRALWADSGIQAAYDRRREFQLGESVKYFLDDMDRIGQQDYLPTQQDILLARKPTKGIHEYDFEIKNVPFKMVDVGGQRSERRRWFECFDCVTSVLFLVSSSEFDQVLMEDRQTNRLMESLNIFETIVNNRVFSNASIILFLNKMDLLEEKVKIVSIKDYFPEYTADPHDLSEVKKFLVECFRSKRREQLKPLYYHFTTAINTENIRLVFRDVKDTILHENLKQLMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KE04</accession>
    <name>A0AAR2KE04_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Neutral alpha-glucosidase AB</fullName>
            <ecNumber evidence="14">3.2.1.207</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Alpha-glucosidase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glucosidase II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GANAB</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55996"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="Rhea" id="RHEA-COMP:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59080"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="EC" id="3.2.1.207"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:56000"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59080"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.207"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.207" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KE04"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059051.1">
        <property type="protein sequence ID" value="ENSPNAP00000062563.1"/>
        <property type="gene ID" value="ENSPNAG00000023237.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159139"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106407">
        <property type="term" value="F:Glc2Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033919">
        <property type="term" value="F:glucan 1,3-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000046">
        <property type="entry name" value="Glucosidase alpha, neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000039">
        <property type="entry name" value="Glucosidase, alpha neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1760:FF:000002">
        <property type="entry name" value="neutral alpha-glucosidase AB isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000023">
        <property type="entry name" value="neutral alpha-glucosidase AB isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF162">
        <property type="entry name" value="NEUTRAL ALPHA-GLUCOSIDASE AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043400609" description="Neutral alpha-glucosidase AB" evidence="18">
        <location>
            <begin position="26"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="20">
        <location>
            <begin position="82"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="19">
        <location>
            <begin position="396"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="21">
        <location>
            <begin position="731"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="944" mass="107544" checksum="6EE28330ECE2E76D" modified="2024-11-27" version="1">MSFPWFRMGLLLGLCISLLLGGSWAVDRGNFKTCDQSAFCKRQRALKPGQSPYRALLDTLELSDSRLTLQLINDNNKVHLLLELYRLQGNITRVKINELKPLKPRYEVPDVLVGDQPTEPLSVLSQDDNGVVLSLGAESQRLIVSARPFRLDIMEGPEVLLSLNSRGLLAFEHLRLRKDTFVKHWRLNPLSLHSSSFTLFCHLFSLFPLMHQSCHLYLCQQAEDGGKDKEEDGLWEETFKTHTDSKPNGPTSISLDFSLPGVEHVYGIPEHADNLKLKTTDGGEPYRLYNLDVFQYELYNPMALYGAVPVMLAHNAQRTMGIFWLNAAETWVDISSNTAGKTVFGKMLDFVQGSSETPQTDVRWISESGIIDVFIMLGPKPSDVFSQYASLTGTQSFPPLAGLGYHQCRWNYNDQDDVKAVDQGFDQHDIPYDFIWLDIEHTDGKRYFTWDPEKFPQPKEMLHGIKDKRRKMVAIVDPHIKVDSGYKIHSEIRARDLYVKNKDGGDYEGWCWPGNSGYPDFTNPEMRAWWASMFAYDKYEGSMENLYTWNDMNEPSVFNGPEVTMYKDAMHGKWEHRDVHNLYGLYVQHMATTDGLIQRSGGTERPFVLTRAFFAGSQRYGAVWTGDNAAEWDHLKISIPMCLSLGLVGISLCGADVGGFFKSPSAELLVRWYQTGAYQPFFRAHAHLDTPRREPWLFGPENTALIRDAIRQRYTLLPYWYQQFYQAHKTGQPVMRPLWVEYPKDTATFTIDDEFLIGQDLLVHPVTEEGARGVTAYLPGQGEVWYDVHTFQKHNGAQNLYIPVTMSSIPVFQRGGSIVPRKVRVRRSSDCMENDPYTLYVALSPQRVAVGELYIDDGHTFNFQTKKEFIHRSLTFANNALSNFSTDSWIEKVLILGASKPNKVTLQMDGKETSVEFEFEASMSVLTLRKPGMNAGADWTVVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KH25</accession>
    <name>A0AAR2KH25_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NAD(P)(+)--arginine ADP-ribosyltransferase</fullName>
            <ecNumber evidence="11">2.4.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Mono(ADP-ribosyl)transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KCND3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-arginyl-[protein] + NAD(+) = N(omega)-(ADP-D-ribosyl)-L-arginyl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19149"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:15087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142554"/>
            <dbReference type="EC" id="2.4.2.31"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Arg-specific ADP-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.31" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KH25"/>
    <dbReference type="Ensembl" id="ENSPNAT00000075013.1">
        <property type="protein sequence ID" value="ENSPNAP00000063585.1"/>
        <property type="gene ID" value="ENSPNAG00000036402.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234601"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106274">
        <property type="term" value="F:NAD+-protein-arginine ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.176.10">
        <property type="entry name" value="Toxin ADP-ribosyltransferase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050999">
        <property type="entry name" value="ADP-ribosyltransferase_ARG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000768">
        <property type="entry name" value="ART"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10339">
        <property type="entry name" value="ADP-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10339:SF25">
        <property type="entry name" value="SECRETED EXOENZYME S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01129">
        <property type="entry name" value="ART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00970">
        <property type="entry name" value="RIBTRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51996">
        <property type="entry name" value="TR_MART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0843">Virulence</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044959486" description="NAD(P)(+)--arginine ADP-ribosyltransferase" evidence="11">
        <location>
            <begin position="22"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="308" mass="35509" checksum="45F9ABBA82013F16" modified="2025-10-08" version="2">MNSTAMMRLFILLILTVHTDSVMNELIMTFACFQITMDLYLNSIDDQFMGCKAKMYCVVTEQLLKNELDSSENFRKDWNAARKKLHLLNTPDSELTETDMTNIALQAYTRNDVYKELNDKMGEGRGTYKTRFGLISLHFLITYGIQTRNAQHECRTTYRRSKIPIVIRGPSVRFGSFASSSLYATLTHFGKETCFIITTCYGADISDISLLPHEAEVLIPPYEVFKNEPVTEIPEDFKDCTRIYTLRSVGKISIMNCEYLKKDKMGPMYMNWTQSSLFLYISTRLTSSFRTSYLSNSTQLKLITHILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHL2</accession>
    <name>A0AAR2KHL2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Sidekick cell adhesion molecule 2a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the sidekick family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561437.2">
        <property type="nucleotide sequence ID" value="XM_017705948.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000045912.1">
        <property type="protein sequence ID" value="ENSPNAP00000061927.1"/>
        <property type="gene ID" value="ENSPNAG00000023248.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432234"/>
    <dbReference type="CTD" id="561852"/>
    <dbReference type="GeneTree" id="ENSGT00940000155761"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000202">
        <property type="entry name" value="Sidekick cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000253">
        <property type="entry name" value="Sidekick cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000158">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000177">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000206">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000209">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000231">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000236">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000237">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000261">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000266">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000267">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000271">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000301">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000359">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000360">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000420">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000434">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000485">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF154">
        <property type="entry name" value="PROTEIN SIDEKICK-2-LIKE ISOFORM X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 17" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043647327" description="Sidekick cell adhesion molecule 2a" evidence="17">
        <location>
            <begin position="27"/>
            <end position="2198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1936"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="32"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="221"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="314"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="408"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="502"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="598"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="699"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="800"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="903"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1001"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1105"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1208"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1309"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1408"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1510"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1632"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1732"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1830"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="885"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1192"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2017"/>
            <end position="2036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2048"/>
            <end position="2075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2100"/>
            <end position="2198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="2049"/>
            <end position="2067"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="2125"/>
            <end position="2144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="2159"/>
            <end position="2170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="2183"/>
            <end position="2198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2198" mass="242630" checksum="DE62ACB56A62C55C" modified="2024-11-27" version="1">MPRRTFTRTWGALWMPLLAVICGARAQDDVPPYFKMEPVATQVHLEGNRLVLTCMAEGSWPLEFKWLYNGTELTRFSLEYRYLIPSLDRSHAGFYRCIVRNRVGALMQRSSQVQVAYMEGFTEGERSQVVSQGEAALIFPPRIPSFPRPQITWFRDGRKIPPSSRIALTLDNTLVILSTVAPDAGRYYAQAVNDKNGENKTSQPVILNVENVGGPADPIAPTIIIPPKNTSVMVGTFEVTLECVANARPLIKLSITWRKNGVVVNRGLRDFGRRLTISGPLVSDSGYYECEASLRSSSVPSVTAGAYLHVLEHPQFVKEPERHITAEMEKVVDIPCQARGVPQPDIVWYKDAVPISPVKTPRYRVLVGGSLQINGLLPDDTGMFQCFARNLAGEIQTNTYLAVTSIAPNITAGPTDSAVIDGMSVILHCETSGAPRPAITWQKGERVLASGSVQLPRFTLLESGSLLVSPSHLSDAGTYTCMASNSRGIDEASANLVVWARTRITNLPQDQSVIKGTKATMSCGVTHDPSVTVRHIWEKGGLAIDVKSSPRLRVDPNGTLHISQTWSGDIGTYTCRVTSVGGNDSRSAYLRVRQLPHAPENPVAMYSSTEKRSINLTWAQAFDGNSPLIRYILEVSENNAPWTILLANIEPEATSIGVGGLIPARSYQFRLCAVNDVGKGQFSRETERLTLPEEPPSAPPQTVIASGRTNQSIMIQWQPPPESHQNGPLQGYIIRYCLSGLPVDCQIKNITNPDQTNLLLEDLIIWTNYEIEVAAYNGAGQGVFSQKVTEWTLQGVPTVPPGNVQAEAVNSTTVRFTWSAPSPQFINGINQGYKLLAWEPGHEDDIAMVTVRPNFQDSVHVGYITGLKKFTEYFTSVLCFTTPGDGPRSPAQRVRTHEDTPGPVGHLSFTEILDTSLKVSWKEPHEKNGLLTGYRISWEEFNRTNTRVTHYLPNVTQEYRVTGLTALTTYTIQVAGMTSKGQGQLSSSTISSGVPPEMPGPPTNIAISNIGPRSVALQFKPGYDGKTSISRWVVEAQIGVIGENEEWVTVHQLSNEPDARSLVVEGLNPYTFYRFRMRQVNIVGTSPPSQPSRKIQTLQAPPDIAPANVTLRTASETSLWLRWVPLPEWEYNGNPELVGYRVQYSRANSKAGVLSHIISDRLEREFTVEDLEEWTEYEVRVQAVNGIGSGPWSQPVHARTRESVPSSGPANVSAFATTSSSVLVRWGEVPEPDRNGLILGYKVVYREKDSDSALHFWTVEGNTSHSVQLTGLDKYVLYEIQVMAFTRIGDGVPSSPPILERTLDDVPGPPVGILFPEVRTNSVRLIWQPPAQPNGIILAYQITYRRNSSNSNSATVDVLSPSARQYTATGLKPESVYVFRLTAQTRKGWGEAAEALVVTTEKRARPQPPSRPVVPQDDVQARSVLLSWEPGSDGLSPVRYYTVQYKELPSNNWTVHSASVNHEASSYIVDKLKPFTSYQFHVKATNDIGDSEYSEESEAITTLQDAPDKAPTILSVTPHTTTSVLVRWKPPTEEQINGILLGFRVRYRELHYDRLRSFTVRTVNNPSANWAELTAPYSVRNLSESSLTQYELDNLSKHKRYEIRLSVYNAVGEGPISSPQEVFVGEAVPTAPPQNVAVQSSTATQLDVTWDPPPLEAQNGDIQGYKIYYWEFQLQNETERLRTLFLPELGVKLKNLTGYTTYMISVAAFNAAGDGPRSPPTRGRTQQAAPSAPSFIHFSELTTTSVNVSWGEPKQPNGIIEGYRLVYEPCTPVDGVSKVVTVDVKGSAPLWMKIKDLADGVTYNFRIRAKTLTYGPEVEANITTGPGEGAPGPPGEPFISRYGTALTLHWTSGDPGRAPITRYVIEARPSDEGLWDILIKDIPKEVTSYTLNLDMLREGVTYDFRVIAVNDYGYGSPSAPSPSISAQKVSPFYEEWWFLVVIALVGLIFILLLIFILVIRGQSKKYTKKTDSGGHSNCTSLPLSHGEMMRLDEGRFPALELNNRRLSVKNSFCRKNGIYTRSPPRPSPGSLHYSDEDVSAKCNDLIPAESSSLTEKPSEVSDSQGSDSEYEMDPNRQKTHSFVNHYISDPTYYNSWRRQQKGVSRPPAYGYGQPDGVAEQESRPHPPPLPPLPPLLPHSTPSPQPQGHQGTLFRPKGSRTPTPSLLSSEAHSQHALYRPPSSLGASTQTPATGFSSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KKM7</accession>
    <name>A0AAR2KKM7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipase A2</fullName>
            <ecNumber evidence="8">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017563008.1">
        <property type="nucleotide sequence ID" value="XM_017707519.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KKM7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050717.1">
        <property type="protein sequence ID" value="ENSPNAP00000062671.1"/>
        <property type="gene ID" value="ENSPNAG00000033836.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108433143"/>
    <dbReference type="CTD" id="8399"/>
    <dbReference type="GeneTree" id="ENSGT00940000157803"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000001">
        <property type="entry name" value="Basic phospholipase A2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF4">
        <property type="entry name" value="GROUP 10 SECRETORY PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043112513" description="Phospholipase A2" evidence="8">
        <location>
            <begin position="17"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="53"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="70"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="153" mass="17404" checksum="B128425612E3B6E4" modified="2024-11-27" version="1">MTALYRTFLLFSVSMASLVPQRPIRNKRGLLELAGVIKCSTGRSALAYMMYGCYCGLGGQGWPRDRADWCCHKHDCCYGDAEFAGCETRTHKYQWTCEGKEADCDSLEDKCEKILCQCDREAAMCLRNSPFNKKYALWPDFMCGCVHPTCNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KLY9</accession>
    <name>A0AAR2KLY9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF268</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KLY9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079455.1">
        <property type="protein sequence ID" value="ENSPNAP00000065353.1"/>
        <property type="gene ID" value="ENSPNAG00000036445.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161335"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004935">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000012">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001155">
        <property type="entry name" value="Zinc finger 30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002604">
        <property type="entry name" value="Zinc finger protein 715"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043602475" description="C2H2-type domain-containing protein" evidence="13">
        <location>
            <begin position="19"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="547"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="577"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="605"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="633"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="661"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="717"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="745"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="802" mass="93934" checksum="61CADD04FCEA7879" modified="2024-11-27" version="1">MYADLLLHLLTQLKSVAGNTSGYSTKRDVKLLKQVKYKTVVSKRHSGIQQSSSGPLHMNTDKNKHSFFFFRLLPSGVATMDGRQKQTQQGEKSLLLRGGKSFTVQTRLKIHTGQKPYHCSECGKSFTSNLQQHQRIHTGEKPYQCTECRKSFTVQTTLQIHQRIHTGEKPYHCSECGKCFNHRSTLQQHQRIHTGEKPYHCSECGKSFSQQSHLKQHQLIHKEKKLYECSECRKSFTLQSHLQQHQRIHTGEKPYDCSECGRSFNRQSTLQEHQRIHTGFKPYYCSECGKSFTQQSSLHRHQRIHTGEKPYHCSECGKSFTQLCHLQEHQRIHTGEKPYHCSDCGKSFRLAQAQCRELHGMGFHELNVIYKNMASVKHPVTKQKSSGTFHTNTSHRQTQTWSKERNHHCLECEKSFTQLSNLRRHQRVHTGEKPHHCSECGRSFTRRGTLKRHQRIHTGERPYQCSECGKTFNHQSHFKTHQLIHAGIKPYHCSECGKNFSQPSSLHQHQRIHTGEKPYHCLECRKSFNQRGSLQQHQRIHTGEKPYYCSECGKSFSFNQRSHLQQHKRIHAREKLYHCSECGKSFTRQATLQIHQYIHTGEKPYRCLECGRSFNQSSHLRQHKRIHTGEKPFYCSECGKSFTRQATLQIHQYIHTGEKPYRCLECGRSFNQQCHLRQHKHVHTGEKPYYCSECGRSFSRQSHLQQHQHIHTGEKPFHCSECGKSFNQKSNFQQHMHIHREQKPYHCLECGMSFNRLNHLQRHQRIHTGEKTYYSSECEKSFRHSKTLKAKKCIKSSDGNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNE9</accession>
    <name>A0AAR2KNE9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNE9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072414.1">
        <property type="protein sequence ID" value="ENSPNAP00000065863.1"/>
        <property type="gene ID" value="ENSPNAG00000037462.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000076382.1">
        <property type="protein sequence ID" value="ENSPNAP00000066960.1"/>
        <property type="gene ID" value="ENSPNAG00000030726.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272266"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF29">
        <property type="entry name" value="SI:CH211-196P9.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="173" mass="19037" checksum="AF283F9394AE9CC3" modified="2024-11-27" version="1">MSSDERQSSSESSFCSEESASSGSTLILESTKARRRQLIDGPPGGDQIFQEYHKTSGLSDGTRRKMVNILVADMVEGHGRIPPVNVRISYALGIITLFPHLKDLDSVNGYVAQDFTLLFGEEVSGRFLAKWPTIFKPKIIADCKTIPSNSHVDDLLALAFQESDHGNVILHEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KPS0</accession>
    <name>A0AAR2KPS0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">C11orf86</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KPS0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086920.1">
        <property type="protein sequence ID" value="ENSPNAP00000064121.1"/>
        <property type="gene ID" value="ENSPNAG00000030377.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234589"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001918">
        <property type="entry name" value="Immunoglobulin light 1 constant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="8"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="110" mass="12055" checksum="86A53AAC14D41A7D" modified="2024-11-27" version="1">FPKGLSAPTLTVLPPSSMELQQGKATLVCLANKGFPSDWRLSWKLDGRSWSSGVSQSPGLLQKDDGLYSWSSTLTLQGDQWLKKTVTCQATKDSQPPVSQTLRREQCTGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KV70</accession>
    <name>A0AAR2KV70_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Isoleucine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">IARS1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="4"/>
    <dbReference type="RefSeq" id="XP_037388270.1">
        <property type="nucleotide sequence ID" value="XM_037532373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KV70"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065944.1">
        <property type="protein sequence ID" value="ENSPNAP00000066297.1"/>
        <property type="gene ID" value="ENSPNAG00000008441.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431535"/>
    <dbReference type="GeneTree" id="ENSGT00550000074921"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000056">
        <property type="entry name" value="Isoleucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000414">
        <property type="entry name" value="Isoleucine--tRNA ligase, cytoplasmic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000004">
        <property type="entry name" value="Isoleucyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000050">
        <property type="entry name" value="Isoleucyl-tRNA synthetase,cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057033">
        <property type="entry name" value="Ubiquitin_IARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23567">
        <property type="entry name" value="Ubiquitin_IARS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="18">
        <location>
            <begin position="18"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="19">
        <location>
            <begin position="693"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="Isoleucine--tRNA ligase cytoplasmic ubiquitin-like" evidence="20">
        <location>
            <begin position="1082"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="domain" description="Isoleucine--tRNA ligase cytoplasmic ubiquitin-like" evidence="20">
        <location>
            <begin position="1174"/>
            <end position="1263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF23567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1266" mass="144677" checksum="CDFE031C3D240A58" modified="2024-11-27" version="1">MVEPVPESIHFPSEEERILQFWKEKDCFQECLKQSKNRPRYTFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGIAEYNKQCRSIVMRYSNEWETSVMRMGRWIDFKNDYKTLYPWFMETVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVIVNFPLLDDESVCLIAWTTTPWTLPSNLALCVNPEFMYVKVKDNTTEKVYIMMEARLGALFKSESEYTILDKFPGKTLKGKRYKPLFEYFIKCKENGAFSVVLDNYVREEEGTGVVHQAPYFGADDYRVCMDYNIIQRDSAPICPVDSSGCFTPDVTHFAGQYVKDADKHIIKFLKENGRLVNASTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEKLLDNNGKCYWVPEFVREKRFGNWLREARDWAISRNRYWGTPIPLWVSDDFEEVVCVGSVVELEELTGVKVTDLHRESIDGLTIPSRCGKGQLKRVSEVFDCWFESGSMPYAQVHYPFENRREFEDTFPADFIAEGIDQTRGWFYTLLVLSTALFGKPPFKNVIVNGLVLASDGQKMSKRKKNYPDPSLIVQNYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVFLPWYNAYRFLVQNVQRLHKEEDIQFLYNENTAKPSDNIMDKWIQSFTQSLIQFFKTEMEAYRLYTVVPKLVKFVDMLTNWYVRTNRRRLKGESGTEDCLWALETLFSVLFSMCRLMAPFTPFITEMIYQNLCHLIDPASVEEKDSSSIHYLMLPQVRESLIDKRIESAVSQMQSVIELGRVIRDRKTLPVKYPLREVVVIHQDPEALRDIQSLQKYILEELNVRQLTLSTDKDKYGIRLRAEPDHMVLGKRLKGAFKSVTASIKELKSEQLEAFQKTGSIVVDGHELHEEDLRLMYSFDQMSGTAVQYEAHSDAQVLVLLDITPDQSMVDEGVAREVINRIQKLRKKGHLVPSDEITVYYSSEPAGDYLDKVIQAHTDFILATTKAPLKPYPVPKNASVIVQERAQLKGSDLDLTIVKGAAVSRAPLTGPACAYVNLKVSLNNKQQEGTVLLENPKGDNKLDVEKLKMVCRSIFKLNDTPLAVFSGTSELKVNSDLLALSGKTVSVTAGASPKATNGSDSVICPYINLQLVNASPAECLSGDVGTLLLANPAGQNGLQHAGLLSETAKVFGLRGRRLKLYKDEDLAEEFTETSLKTLNTKTLYVRVLPTTAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KWR3</accession>
    <name>A0AAR2KWR3_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KWR3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045882.1">
        <property type="protein sequence ID" value="ENSPNAP00000068705.1"/>
        <property type="gene ID" value="ENSPNAG00000034641.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000274369"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043534924" evidence="2">
        <location>
            <begin position="16"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="197" mass="19589" checksum="68EC434250C9A13C" modified="2024-11-27" version="1">MGLLFLLSLFPVALLCLLSLFPRGPPVSPVSVPRGPPVSPVSVPRGPPVSPVSVPCGPPVSPVSVPRGPPVSPVSVPRGPPVSPVSVPRGPPVSPVSVPRGPPVSPVSVPCGPPVSPVSVPCGPPVSPVSVPRGPPVSPVSVPRGPPVSPVSVPRGPRVSPVSVPCGPCVSPVSVPRGPRVPAPCFGDSSPDYFLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KXV0</accession>
    <name>A0AAR2KXV0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein light chain Tctex-type family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KXV0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061007.1">
        <property type="protein sequence ID" value="ENSPNAP00000067212.1"/>
        <property type="gene ID" value="ENSPNAG00000035677.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160185"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21451">
        <property type="entry name" value="DLC-like_TCTEX1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.40">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005334">
        <property type="entry name" value="Tctex-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038586">
        <property type="entry name" value="Tctex-1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255:SF7">
        <property type="entry name" value="DYNEIN LIGHT CHAIN TCTEX-TYPE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21255">
        <property type="entry name" value="T-COMPLEX-ASSOCIATED-TESTIS-EXPRESSED 1/ DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03645">
        <property type="entry name" value="Tctex-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="121" mass="13853" checksum="79ED2DF71E7D7C35" modified="2024-11-27" version="1">SRVTIKAENDSKQQRNRTKKNAGAVKTEESIPWTRSITIHPGESAWRPYQTLSDSIKDAAKRLSYERYKLISYVAIGQLRDSDVTCSSRGVWCPAADTFTEYVFKNEHLFALCILFAVYQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L1W3</accession>
    <name>A0AAR2L1W3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">XK-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">XKR4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Phospholipid scramblase that promotes phosphatidylserine exposure on apoptotic cell surface. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the XK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017561475.1">
        <property type="nucleotide sequence ID" value="XM_017705986.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L1W3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082335.1">
        <property type="protein sequence ID" value="ENSPNAP00000070603.1"/>
        <property type="gene ID" value="ENSPNAG00000036184.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108432262"/>
    <dbReference type="CTD" id="114786"/>
    <dbReference type="GeneTree" id="ENSGT01140000282533"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902742">
        <property type="term" value="P:apoptotic process involved in development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043652">
        <property type="term" value="P:engulfment of apoptotic cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070782">
        <property type="term" value="P:phosphatidylserine exposure on apoptotic cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018629">
        <property type="entry name" value="XK-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050895">
        <property type="entry name" value="XK-related_scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024:SF16">
        <property type="entry name" value="XK-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09815">
        <property type="entry name" value="XK-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="170"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="623" mass="69928" checksum="D8020435CAEF7EC5" modified="2024-11-27" version="1">MAAKSDGVLKMKKSDVAFTPLQNSDHSGSVQGLAPGSQPDSGAGEADFNVNGGSRCCGGGGSNSTCLRLGRERQRYTVWDCLWILAAVAVYFADVGTDVWLSVDYYLRRDYWWFGLTLFFVVLGSFSVQVFSFRWFVHDFGAADGSSSSSGCSHMDGKLLSCSASHGDVGAAHPSTPQRQASTASKSNTNTTATNSSSNSSSSTTNAARTNQKRSASCSFCIWLMQSVIHILQLGQIWRYFHTIYLGIRSRRSGENDRWRFYWRMVYEYADVSMLHLLATFLESAPQLVLQLCIIIQTHKLQAVQGMTAAASLVSLAWALASYQKALRDSRDDKKPLSYLAVIIQFCWHFFTIAARVVTFALFASVFQLYFGIFIVLHWCVMTFWIVHCETEFCITKWEEIVFDMVVGIIYIFSWFNVKEGRTRCRLFVYYLVILLENAALSTLWYLYRIPASTDAFAVPALCVIFSSFLTGVVFMLMYYAFFHPNGPRFGRSSSLTQLDEASAAFAMPSEGATNSLRSNRGGTLARDADREAKYGTERDGCVPVFQVRPTAPSTPSSRAPRLEETVIRIDLCRNRYPAWERHVLDRSIRKAILAIDSSPTPPRLQYKDDTLVQERLEYETTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L9Y3</accession>
    <name>A0AAR2L9Y3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Family with sequence similarity 155 member A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NALF1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NALF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L9Y3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076106.1">
        <property type="protein sequence ID" value="ENSPNAP00000073403.1"/>
        <property type="gene ID" value="ENSPNAG00000036246.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155696"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015275">
        <property type="term" value="F:stretch-activated, monoatomic cation-selective, calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055288">
        <property type="entry name" value="NALCN_aux_factor_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819:SF9">
        <property type="entry name" value="NALCN CHANNEL AUXILIARY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN FAM155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="45612" checksum="DA57E8D129C5D37A" modified="2024-11-27" version="1">MTSGARKARHRQRDDGSKTWTCCWCWWWWCWWWWCARGQSSDHNEDDTPPLFTDCERAQRWRRSLASLLFLTALLSDRLGPGAEARLTGSLDKVMSANLGTGTGAGTGREPGILRSNEGTTFVGNSSGASARGPEKKTCAAKNCLTFVDADHLCRGLWGASEWNASAADLYLSFCDSYSLMDLLDGSSSPDRLNCSVDAMRAGGVGRCSLCVQAYQRYDLHAQEKYEEFELMTDRYVTDAYSVRTCMEECKMVYKPWLCAQYFQSNQSSCSRKVPCEQYCLEVQQRCPFILPDNDDLIHGGTPSFICTGLSETASAEQEPQCCDVRWELNASNRSRDTFKRTHPSCQHSSSVTTAAASASSGAPRLGQGRLKLCLLVLVLLHTVATMTASQNSTCLAGIFPLEDNNAPREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LBW4</accession>
    <name>A0AAR2LBW4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LBW4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062885.1">
        <property type="protein sequence ID" value="ENSPNAP00000074093.1"/>
        <property type="gene ID" value="ENSPNAG00000036411.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271879"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="376"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="846" mass="97587" checksum="F9C55DE7B4906FA2" modified="2024-11-27" version="1">MSFIVICLYRPPSSNSLFYEHLYSILKECDEKKELILMGDFNINWENKQDRKRLKEITDKFSLTQLVQGPTRVTKCSQTQIDLMFTNRQERITKSSNLLTGLSDHNFILVVRKLTKKRFKNIIKPKHYYQKIPKSEMHNLENALKQIDWLNILSCKDIDDNCDIFLSTINKVMSSFTRKVQSKPRQRETLPWLNDALLQQMKERDFALKRTLKTGLNNDRLIFTSLRNKVVRNIRKAKAEFFIKIIDNARGNGKMIWQNLNKLTGRNNDQRTKEYELKVNGTLIQDHDMIANTFNNFFINSIEQLTQSFSSKTIISTIIDDDIPIFRIMETTESAVLNIINSLRDSKAKDVYGLDTTFLKLHKGILASPIAKLINHSIRQGIFPSAWKSAVITPIFKAGDRTVVENYRPISILPAVSKVAEKWVVQQLTAHLNKSYTPLHSMQFGFRSNHSTETAICYFLEGVKSKLDKGGVIGAVFLDLKRAFDTVNHKVLLSKLSCFNFSIDSVKWMNSYISNRKQRVRIGKTQSTYLSCNTGVPQGSVLGPILFSLYINDLPSVCSSVNIQMYADDTVLYVHAKNKQQAASELTAAMVLISDWLSNSCLYLNINKTVCMFFSQKLTEMIQADVLVRGEKLKLVSDTKYLGIILDSNLSFKNQVKKVINTVKLNLANFRHIRPYLTTEAAKLFMHAMIVSHITYCFTSWSQTSLSILKPIESLYKQALKILDRKPNSYHHCHIVQKYNLLSFDSFKHLLDACLMFKILYGLAPPPLRQFIKQKDSRGSMVTRAFTRGDCIVQYRRSKFGQTVFSIRASQYWNSLPTELRTCTNYLIFKYKLKTWLKANQICNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LHF3</accession>
    <name>A0AAR2LHF3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Early endosome antigen 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">EEA1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSPNAT00000069164.1">
        <property type="protein sequence ID" value="ENSPNAP00000073816.1"/>
        <property type="gene ID" value="ENSPNAG00000018513.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156910"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.390">
        <property type="entry name" value="L1 transposable element, trimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164:SF30">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69979">
        <property type="entry name" value="Eea1 homodimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1270"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1295"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1333"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1373"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1415"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1459"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1534"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="616"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1065"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="767"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1066"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1559" mass="176139" checksum="66B0625981BCF719" modified="2024-11-27" version="1">MEIHSSVISCVFSELEMKLHEAETEKFNIKQMKDLFEQKAAQLATEIVDIKSRYDEEKSLREVAEQRLASLQQDLQRERQEKDKLSTELLQRPGVEDVEVLKRELVQVQTLMDKMTREREEESERLKNKYEQLQANFTTSEIRKLETTITQLKAELEKGPQEAAVYTQQIHQLQSNLNNLQQQSQLLSEKLTRKEKENQELDERLGQEQASKKNLQANLHQRELELQEVQARVSAGENSLGRTQSELAERGEEAARLRRELTELEKSYQELKAERKQLQQQREERESQGLQQQSEISQLHAKLLEAERQLGEVQGRLKEQRQLSGEKLKDREQQVADLQLKLSRLEEQLKEDATKSTDLQHQLDKSKQQYQELQALQQSTNGKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKASLSQLEAEREDLCAKIQAGEGEAALLNQLQEKNHMLQEQITQLTDKLKNQSESHKQAQENLHEQLQEQKSQLRSAQDRCQGLESTVTDLTAQLTQSKERVAQLDTQLKAKTEMLLSAEAAKIAQRADLENHLETAQNALQDKQQEVSKVQAQLEEQGHRLQEKQEQCSQLEASLKESKDRLLAAEQRIDTLESQAKKAEAELTELRSGKEQAQKEQQRQQQHISELEQKNKELSRLLDTEKQGAAALQEAVQKKSSALADTRKHLEKLEKEKIGLQTSLDKLTKEGQARQAELEKKLQGQNVELQKAHQERDMQAKELVSVKDALSKASAAFKENQTRLEKEKKTSIAALEEKEKAHKVAQQELQKSAEATAKDLSTAKSQLEKMAEVEKGLQAQVAAANAQLKQTQDVLKEREKEVQQLQAQLKTAQESFNKEAKKLQTQVAELQTAQTQKVEELTKLKEQMAGLSKELSSEKSLSTELQKICDNTKESLAKLQSDYYGKESEVSALRQDLKVCDDKLALAQEELVASTNQLNNREAQIQELKAGFAALEKDLSKRDENLKQQEEALKELQKQKELTQEELKKAQSQVEQLRAAHAALEKDRSKLSAELKALAEKNEKEVKELQAAKQLLLQQKVDIQGKVEATQAALEKEQREHQTTRESVTQTRQQHKAETDKLHKQLALEVKAKEEVEKQREEAETKLSMQVSALNENVATLKREWQSSQRRVGELEKQTDELRGEIAVLEATVQNNQDERRALLERCVKGEGEIEKLQAKVVEMRRKLDDATAAMQELGRENQSLQIKQSQSLTRKWAEDHEVQNCMACGKGFSVTIRKVTFVLSLPSFVSVLPPPSSLNAIFPLILFLACSLARLRSLSLSLSLSLSLSLLLFLSLSRSLSVTLSLSLSLSLSRSLSLCLSLSLSLLLCLSLTVALCLTVALSVCLSLSLSLSLSLSLSLTLSLSLSLSLLLFLSLSLSLSYSFSLSLSLSLSRSLSLSLSLSLSLCLSLTVALCLSLSLSLSLCLSLTVALCLTVARSLSLSLSLSLSLSLTLLLSLSLSLSLSLSLSLSLSLSLSLSSHNHTQHTDWLSYLMELFVLSIKAPNQYYVNGLPQGPTFIQNPFKVLCFFFFFFPTAVNRQYYVETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJK5</accession>
    <name>A0AAR2LJK5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Denticleless E3 ubiquitin protein ligase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DTL</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat cdt2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037393602.1">
        <property type="nucleotide sequence ID" value="XM_037537705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJK5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073177.1">
        <property type="protein sequence ID" value="ENSPNAP00000074934.1"/>
        <property type="gene ID" value="ENSPNAG00000012221.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426253"/>
    <dbReference type="GeneTree" id="ENSGT00530000064210"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002879">
        <property type="entry name" value="Denticleless protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051865">
        <property type="entry name" value="WD-repeat_CDT2_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852:SF0">
        <property type="entry name" value="DENTICLELESS PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852">
        <property type="entry name" value="LETHAL 2 DENTICLELESS PROTEIN RETINOIC ACID-REGULATED NUCLEAR MATRIX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="133"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="353"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="435"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="674" mass="73794" checksum="F55A9A2BA7866119" modified="2024-11-27" version="1">MLFQCVVDRGVTKRRNVNRREFPLSSLLEGYQCARHDEHISYGSTALAVPPFGCSFSTAPGQSNTLAVANEEGVVCIYNTAEKQRAVLREWPAHDNAVFDIAWVPGSNSLVTASGDQTARLWDVLTEDLLGSFKGHQCSLKSVSFPKQEKALFCTGGRDGNIMVWDTRCSKKDGFYRQVKQISGAHMKTEGNTPQTKKKRASSRGMAPSVDSQRGVTVVLFRDENTLISSGAVDGTIKMWDLRKNYTAYHQNPTPLQMYKYPGSSTRKLGYSGLTLDSTGSRLFCNCTDDNVYMFDISGLKTAPVAVFSGHRNSSFYVKSSVSPDDQFLASGSSDFHAYIWKISNPAQAPVMLQGHSQEVTSVTWCPTDFTKIATCSDDNTVRIWRLSRGVEGERSSIGEANLVGWAQRKIQTPSRSSGHFTHVELTPARISSMASVGGLASPQPGSCPSSNADLPPASSTSAPAHRNSPSHPKTPKSPQTPSSIRHWVSRTPGSHGNRTTPPLRKVLSPCPQSPERVSPSERKAKRRLETGDTTLDHHDNRNGVAELWPGAKRSRGAAGTFQHSVEEEEEKSESLVMKEEPLTSSVQADKENSSPSKPDWLSAMSRRLKQAQKGAALSKSPNGGKRHESRTQSVSTLSSPHSSRKISSPQAKQTSSPRSMKKISSYFQKMPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LPK3</accession>
    <name>A0AAR2LPK3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Ribonuclease H1</fullName>
            <shortName evidence="15">RNase H1</shortName>
            <ecNumber evidence="6 15">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="6 15"/>
    <dbReference type="RefSeq" id="XP_017537901.1">
        <property type="nucleotide sequence ID" value="XM_017682412.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017537911.1">
        <property type="nucleotide sequence ID" value="XM_017682422.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LPK3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060486.1">
        <property type="protein sequence ID" value="ENSPNAP00000076669.1"/>
        <property type="gene ID" value="ENSPNAG00000022344.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411045"/>
    <dbReference type="CTD" id="246243"/>
    <dbReference type="GeneTree" id="ENSGT00390000003466"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09280">
        <property type="entry name" value="RNase_HI_eukaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000049">
        <property type="entry name" value="Ribonuclease H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.970.10:FF:000001">
        <property type="entry name" value="Ribonuclease H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050092">
        <property type="entry name" value="RNase_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017067">
        <property type="entry name" value="RNase_H1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642:SF26">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036852">
        <property type="entry name" value="Ribonuclease_H1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="17">
        <location>
            <begin position="138"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="92"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR036852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000076669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="288" mass="31959" checksum="5A04A3F0531F7D0E" modified="2024-11-27" version="1">MLRLPTLCSIVRRALCVSAEEMGKKGSFFYAVRKGHNPGVYQTWDECKHQVDKFPCAQYKKFASEVDAWAFVRNEATGLLLTTDSDVQLPKSVPVGAKRPCEDNKDDETNMKRFKVSEEPTQPKASSAAGSTDGFTHMGDAVVVYTDGCCSGNGRNGARAGIGVYWGPDHPLNVAERLPGRQTNQRAELQAACKALEQARDMNIKKLVLYTDSKFTINGITSWVKNWKINGWRLRTGGEVVNKEDFQKLDKLNGELEVIWMHIAGHAGYKGNEEADRLSREGAAKPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M189</accession>
    <name>A0AAR2M189_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M189"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050608.1">
        <property type="protein sequence ID" value="ENSPNAP00000080754.1"/>
        <property type="gene ID" value="ENSPNAG00000032206.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214408"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="328"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="675"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="746"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="802"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="889" mass="100592" checksum="D6DB5A29CFBDA232" modified="2024-11-27" version="1">MRVIAYASRGLSRSESKYPAHKLEFLALKWAVTEKFNDYLYGSNFTVITDSNPLTYILTTAKMDATSYRWLAALSTFTFKLKYRAGKQNSDADGLSRRSHGGLTEDVMSQKERERICQFTHTHLDDSSVSISKDAVYALCEKHLVNNVTSEENSAVALVQSLAVSSDAIPDSFASEDQHGGLPIIPHFSEEEIRDKQRADPCIREVISMIETGEKLVPAIRTELPQITYLLRQLSRLSLHNQILYRRRLEGSTPTYQLVLPEELCPVVLKSLHDDMGHMGIDRTMDLIRTRFYWPKLADDVERKVKTCERCVRRKSLPEKAAPLTNIVSTRPLELVCMDFLSIEPDSKNTKDVLVLTDHFTKYAIAIPTPNQKAQTVAKCLWDNLIVQYGIPECLHSDQGPDFESHVIKELCQLMGIRKTHTTPYHPRSNPVERFNRTLLNMLGTLRDEDKSRWRNFVKPLVHAYNCTRNDVTGFTPYELMFGRQPRLPVDLAFGLPLQSDQRKSHSQYVQELKSHLEDSFKIASLNSEKNAKANKTRFDAHITASKLEAGDRVLVRAVRLRGKHKLADRWETDIHVVVKKAGDLPVYTVRPENHDGPLRTLHRDLLRPCSFLPVSVGRPVTPQRPRRPQTRQFAECNTEDVFSDFEEDFEIHRHGRLPAMTPTRFTAVYEIPRRDESNLPGDDPVNESNLPADGPVDKNDLPVVVPVEDDYLTPGTPVREKTQDQPESQSLHLTFAVDTNVAEPAEADSNANSSEPADEAEPQEMAEQSIRMYEQPEEVSNVPLEESNAVSSDDAATETLDGIRRSTRNRERPDRLQYSRLGHPLISIVQSLFQGLSTAFTDVLQDAQDSEYLLTSCPKLTTKQPPPCTGTCMISRGEGVAQTNNLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M1W7</accession>
    <name>A0AAR2M1W7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2M1W7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068719.1">
        <property type="protein sequence ID" value="ENSPNAP00000080601.1"/>
        <property type="gene ID" value="ENSPNAG00000037112.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164468"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="6"/>
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        <property type="entry name" value="Cadherin_C"/>
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        <property type="entry name" value="Cadherin_CS"/>
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        <property type="entry name" value="Cadherin_N"/>
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    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
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        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR24028:SF296">
        <property type="entry name" value="PROTOCADHERIN 1 GAMMA 11 PRECURSOR-RELATED"/>
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        <property type="match status" value="5"/>
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        <property type="entry name" value="Cadherin_2"/>
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        <property type="entry name" value="Cadherin_C_2"/>
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        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="CADHERIN_1"/>
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        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="691"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="726"/>
            <end position="743"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="813"/>
            <end position="832"/>
        </location>
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            <begin position="62"/>
            <end position="137"/>
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    <feature type="domain" description="Cadherin" evidence="15">
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            <begin position="138"/>
            <end position="246"/>
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    <feature type="domain" description="Cadherin" evidence="15">
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            <begin position="247"/>
            <end position="351"/>
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            <begin position="352"/>
            <end position="456"/>
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    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="457"/>
            <end position="566"/>
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    <feature type="domain" description="Cadherin" evidence="15">
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            <begin position="581"/>
            <end position="678"/>
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            <begin position="834"/>
            <end position="867"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="PROSITE" id="PS50268"/>
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        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080601.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="867" mass="96331" checksum="31B4B502E055941F" modified="2024-11-27" version="1">MLVFLLHFSSTMQLKWQTVFFCLCFYGMDVVFCQARYSIPEEMPEGSFVGNIAKDLGIEISRLISGKARVVTKGGRQYVELSRDKGALVVKERIDREELCKQTTPCSFSFDLIIENPIQLHRITVEVQDINDNSPKFPKSKIDLTISENTASGTRFPLDSAVDLDVGINGIQNYALMPTDNFKLDVNNRADGKKYVEMVLQRELDREERNELKLVLTAYDGGSPKKSSTAQIHISVLDVNDNTPACKQALYKAEVKENSPIGSVLTTVSAADADEGVNGEVSYSFAQASEDARHVFDINSETGQITLLNRLDYETEKSYQLDIKAMDKGTLADTCKVIIDVVDVNDNPPTIQLMSFSNTIAENSPVGTTVAVINVEDADSGQNGVVQCKINEHIPFKIESSLTDYYALVTDEILDRENIAEYNITILVSDQGSPALHSNKTLTVKISDVNDNAPVFGSDQFRTFITENNSPGVAVLTVKAGDADWGPNARLSYFLEDSDVTGNPVSSLVSINSDSGVIHAVRSFDYEQMKSLTFNVTARDGGSPPLSSEVMVTLIVQDQNDNAPQVLYPVQTGGSVVAEIVPRSADVGYLVTKVVAVDVDSGQNAWLSYKLQKATDRALFEVGPQNGEIRTVRQVTDKDAVKQKLTVVVEDNGQPSRSAVVNINVAVADSFPEVVSEFSDFTLSKEYNDNLTFYLVLALAAVSFLFITTVVVIISVKIYRWRQSRIFYQSSLPVIPYYPPGYADTGVTGTLPHMYNYDVCMKTNSRKSDCKYSTLGGQSVLVVDQSFSETLQRAMKEKEFLENAESPEMVGPFFYILSFCGGVEWYILYVALKKGRKKGRKKGRKKERKKERKKERKKERKKERKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M526</accession>
    <name>A0AAR2M526_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Glycine receptor subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Glycine receptor 58 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Subunit of heteromeric glycine-gated chloride channels. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="26">Forms heteropentamers with glycin receptor alpha subunits. Heteropentamers with GLRA1 can be composed of two GLRA1 and three GLRB subunits, or three GLRA1 and two GLRB subunits, or four GLRA1 subunits and one GLRB subunit. Forms heteropentamers with GLRA2. Functional GLRB-GLRA2 heteropentamers contain four GLRA2 subunits and one GLRB subunit, although alternative subunit composition cannot be excluded. Forms a heteropentamer with GLRA3. Interacts with GPHN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Postsynaptic cell membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRB sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037396464.1">
        <property type="nucleotide sequence ID" value="XM_037540567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M526"/>
    <dbReference type="Ensembl" id="ENSPNAT00000072870.1">
        <property type="protein sequence ID" value="ENSPNAP00000082317.1"/>
        <property type="gene ID" value="ENSPNAG00000015085.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411140"/>
    <dbReference type="CTD" id="445193"/>
    <dbReference type="GeneTree" id="ENSGT00940000156344"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016934">
        <property type="term" value="F:extracellularly glycine-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19010">
        <property type="entry name" value="LGIC_ECD_GlyR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19061">
        <property type="entry name" value="LGIC_TM_GlyR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000070">
        <property type="entry name" value="Glycine receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000014">
        <property type="entry name" value="Glycine receptor subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008060">
        <property type="entry name" value="Glycine_rcpt_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047031">
        <property type="entry name" value="GlyR_beta__ECD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047029">
        <property type="entry name" value="GlyR_beta_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01677">
        <property type="entry name" value="GLYRBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0868">Chloride</keyword>
    <keyword evidence="15" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="21 29" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 29" id="KW-0406">Ion transport</keyword>
    <keyword evidence="20" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="12 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="7 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 29" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="31">
        <location>
            <begin position="166"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="32">
        <location>
            <begin position="381"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00060802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00064974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00077540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000082317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="604" mass="68054" checksum="6F554D782A3164E1" modified="2024-11-27" version="1">MTGARTHTLILHLRQATCTRRHSGESNLLPTPEPTPAVRSEPDRTEPDRTVAWGWPARNERGERLDGEQHALSRQTVTPARALLLCIATASENQRDARAMMAVRVSCQKMKFLKLLLLLCVLCLWMGGGSAKEKSAKKGKKKGKQVYCPSQLSSEDLAQVPANSTSNILNRLLISYDPRIRPNFKGIPVEDRVNIFINSFGSIQETTMDYRVNIFLRQRWNDPRLRLPQDFKSESLTVDPKMFKCLWKPDLFFANEKSANFHDVTQENILLFIFRNGDVLISMRLSVTLSCPLDLTLFPMDTQRCKMQLESFGYTTDDLQFMWQSGDPVQMDEIALPQFDIRQEDIEYGNCTKYYAGTGYYTCVEVIFTLRRQVGFYMMGVYAPTLLIVVLSWLSFWINPDASAARVPLGILSVLSLSSECTSLASELPKVSYVKAIDIWLIACLLFGFASLVEYAVVQVMLNSPKLVEAEKARMASREKAEGKTPSKINSLNGTGGTPLHVSTLQVTETRCKKVCTSKSDLRSNDFSIVGSLPRDFELSNFDCYGKPIDVGNAVGKSQAKNNKKPPPPKPVIPSAAKRIDLYSRALFPFSFLFFNIIYWSVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BLM7</accession>
    <name>A0A3B4BLM7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017578399.1">
        <property type="nucleotide sequence ID" value="XM_017722910.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017722911.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017722912.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037396208.1">
        <property type="nucleotide sequence ID" value="XM_037540311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BLM7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000458"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013821.2">
        <property type="protein sequence ID" value="ENSPNAP00000000458.1"/>
        <property type="gene ID" value="ENSPNAG00000005354.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442730"/>
    <dbReference type="GeneTree" id="ENSGT00950000182914"/>
    <dbReference type="OMA" id="SQAFAMF"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17313">
        <property type="entry name" value="MFS_SLC45_SUC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 45 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 45 member 3"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF35">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 45 MEMBER 3 ISOFORM X1"/>
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    </dbReference>
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        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="551" mass="60645" checksum="761FB0C25A3829F7" modified="2018-12-05" version="1">MPGWRSQWRLVLLNLLTCGLEICVAAGITYVPPLLLEAGVEEKYMTMVLGIGPVLGLLFIPLIGSASDHCNSIYGRRRPFIWLLSLGVLLALLIIPHADVLAVHLSWGSSRSNQALQIGLLILGVGLLDFCGQVCFTPLEALLSDLYREREDCAQAFAMFSFMVSLGGCVGYLLPALDWSGGLLAYYLGGQAECLFTLLILIFIVSVLVTMKVSEEPSCRPLVPEAGPSNAGRCGPRSCCYVLQCKLRLLKSGPLLCFLRTCWSMTPAIYRSYCHVPRVMRQLCVAQLCSWMAVMSFMLFYTDFVGEGLYEGVPSAAPGTVLRQRYDEGIRMGSLGLFLQCATSTFFSLVMSRLVHVFGSRKVYLSSMVSFTVSALIICLSKSVLLVTAMSALTGFAYATLQTLPYTLTCHYHKEKEVYMQTKKTHKSGLSITHESVCLTLDDDTGDLNHKSGYANGHVHYDQEYYPSQHSQNGTPPALGSDDYDKRGVGLDFAILDSTFLLSQVFPTFFMGMIVQFTQSVTAYVASSAIFGAIGVYFATHIIFDQKDLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQB6</accession>
    <name>A0A3B4BQB6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Multidrug and toxin extrusion protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550411.2">
        <property type="nucleotide sequence ID" value="XM_017694922.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQB6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001902"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011363.2">
        <property type="protein sequence ID" value="ENSPNAP00000001902.1"/>
        <property type="gene ID" value="ENSPNAG00000000998.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425891"/>
    <dbReference type="GeneTree" id="ENSGT00940000163922"/>
    <dbReference type="OMA" id="CTETWAY"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="521"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="632" mass="68804" checksum="E27F6F1AF312B10E" modified="2018-12-05" version="1">MSLVKERTEEMMDGSSAGAEAPSMEPSSRLFCCGWLRRCLPLTYREELYHILCMTGPLVASRILNYLLPFVITMFCGRLGNSELAGYGIASATISITTAATGTGLALACDTLVSQTFGRKNLLRVGVILQRGILILLLFCLPCWALLINTQPLLLLLGQDPEVARIAQLYVLVHLPAVPAMFLHQLQVAYLQNQGVILPQMYAAAAANIANVLTNYILIVWLDFGLYGSAAASTLAEVYICVFLYAYIRWKRLHTETWGGWSLESLQEWGAYMKLAVPSSMMLCFEWWIYELGGFLAGMLSELDLAAQHAVITLATLNYMFPLGIQGAACVRVGNALGAGDTAGAVLTCKVSLTCTAILAVLQGAVLASLKSFIGFLFTDDRQIVELVSKLMSVYCALEFFDGLVCVCMGILLGSGQQKIAAVANLFGYYCIGLPLGISLMYAAKLEIIGFWLGLLICVCIQSTFFIIVIFKLNWERLTKQAIERAGKYKKSGMISDFESDPNRLMPESLGPMECMSNNAENGNDYQAVSSQDSDDVRSTPVDQERLSISQEGARMHQDGGKSSEPLSTSQLIIRRGLTTLAAVLIFAVGMSIHIIVPLPEVPHALGANATQSNTTLHYTTPSPQTTSLMSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BQP7</accession>
    <name>A0A3B4BQP7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Exosome complex component MTR3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Exosome component 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">mRNA transport regulator 3 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Non-catalytic component of the RNA exosome complex which has 3'-&gt;5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the RNA exosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017581112.1">
        <property type="nucleotide sequence ID" value="XM_017725623.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BQP7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001938"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011288.2">
        <property type="protein sequence ID" value="ENSPNAP00000001938.1"/>
        <property type="gene ID" value="ENSPNAG00000008453.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444441"/>
    <dbReference type="CTD" id="118460"/>
    <dbReference type="GeneTree" id="ENSGT00940000153348"/>
    <dbReference type="OMA" id="MCCVYGP"/>
    <dbReference type="OrthoDB" id="2504340at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11371">
        <property type="entry name" value="RNase_PH_MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000035">
        <property type="entry name" value="Exosome complex component MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="14">
        <location>
            <begin position="42"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="271" mass="29555" checksum="FDCBBDCCAE5F3D18" modified="2018-12-05" version="1">MPVDTKRVRGPDESQSPLVFLPPTAQTCEVLRRPGDRGKGDVRPLFARCGLVSQAKGSAYIEAGNTKIMCSVYGPRETERKDDTDMKSGRLVCDVRLAPFSCQKRGAWIQGSEERDLSLALSESMQPGVCLHRYPRSQIEVNVMVLENDGGVLAHAVTCASMALADAGIEMYDVVLGCALRQDGDSCLVDPDFSEERGSWQAGYGENQGRVTVALLPNFNQVSGLHADGEMREDTLTAAVRTCMDGCHKLYPVVQQAILRAVKRKAPPPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWQ5</accession>
    <name>A0A3B4BWQ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017549648.1">
        <property type="nucleotide sequence ID" value="XM_017694159.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWQ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002976"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009526.2">
        <property type="protein sequence ID" value="ENSPNAP00000002976.1"/>
        <property type="gene ID" value="ENSPNAG00000009329.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425478"/>
    <dbReference type="CTD" id="2701"/>
    <dbReference type="GeneTree" id="ENSGT01150000286954"/>
    <dbReference type="OMA" id="TSVCYDK"/>
    <dbReference type="OrthoDB" id="8878488at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043473">
        <property type="term" value="P:pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF39">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="174"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="319"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="354" mass="41190" checksum="EBCF804E4F7599D0" modified="2018-12-05" version="1">MSRADWGFLERLLVEGQEYSTNVGRVWLTVLFLFRLLVLGTAAESAWDDEQSDFICNTKQPGCEAVCYDKAFPVSHFRYFVLQVIFVSTPTIFYFGYVALKTHEDQQTAEETREEQRGRQRNGKANQSKLDVIKEEDEEDEKQVENKKKQGAPPKLKGKLLSVYAVSIVLKILLEVGFIVGLWFLYGFVVAAKYECKRQPCPHTVDCFVSRPTEKTIFTIYTQIIAAISVFLNVLELFHLLKLAITRSLEKRYQYHELGRQIERTPTKLELAEVHALSYEEKGHLFLPVDNDSYTQSSLDWTEREHHLAEDMLPSYSNCVRNSKPRHQSKKHIHSKKHSRSDHKEKRSKDKYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYF3</accession>
    <name>A0A3B4BYF3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017579023.1">
        <property type="nucleotide sequence ID" value="XM_017723534.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYF3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004608"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006730.2">
        <property type="protein sequence ID" value="ENSPNAP00000004608.1"/>
        <property type="gene ID" value="ENSPNAG00000001501.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443108"/>
    <dbReference type="GeneTree" id="ENSGT01150000286932"/>
    <dbReference type="OMA" id="TIFMVGI"/>
    <dbReference type="OrthoDB" id="10037617at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004962">
        <property type="term" value="F:endothelin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048484">
        <property type="term" value="P:enteric nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15977">
        <property type="entry name" value="7tmA_ET-CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000076">
        <property type="entry name" value="Endothelin receptor type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000499">
        <property type="entry name" value="Endthln_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001112">
        <property type="entry name" value="ETB_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051193">
        <property type="entry name" value="GPCR_endothelin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46099:SF4">
        <property type="entry name" value="ENDOTHELIN RECEPTOR TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46099">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00571">
        <property type="entry name" value="ENDOTHELINBR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00366">
        <property type="entry name" value="ENDOTHELINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017193309" description="G-protein coupled receptors family 1 profile domain-containing protein" evidence="14">
        <location>
            <begin position="18"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="335"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="92"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="418" mass="47260" checksum="9059F1C57178D65B" modified="2018-12-05" version="1">MKAVGLVILLLAGCSWASRFSRDSTQVSLGDAANQEVEAAGWNTSDVEQPVMQSPPRGPFLPMCNKPTEIREAFKYINTVISCLIFVVGIIGNSTLLRIIYKNKCMRNGPNVLIGSLALGDLLYILIAIPINVFKLLAEDWPFGVHICKLMPFVQKASVGITVLSLCALSIDRYHAVTSWSRVKGMEIPLWKILEVTLIWLVSVVLAVPEALTFDMLEFTYRGRKLRVCLLHPQQSTPFMQFYQDVKDWWLFGFYFCLPLACTGVFYTLMSCEMLNRKKGMRIALNDHMKQRREVAKTVFCLVVIFALCWLPLHLSRILKKTIYDPTDPNRCELLSFLLVIDYIGINMASLNSCINPVALYFVSQKFKNCFKSCLCCWCQRNITPIDERGSGIRWKGSCHGNGLDRSNSRSSQKCSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3N0</accession>
    <name>A0A3B4C3N0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MASP2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3N0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003448.2">
        <property type="protein sequence ID" value="ENSPNAP00000006502.2"/>
        <property type="gene ID" value="ENSPNAG00000012586.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="14"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="131"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006502.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="290" mass="32549" checksum="1AD010612EC89FFB" modified="2024-11-27" version="2">MLRLCFCLAGHLQGKILQLDSVVTAKEGDDVTLSCLHPTDQINKVLWYQQPIGQKPLLVASSYYHSLPFEFYNDFDKTKRFDLSRRSGSSNLTITKVEPSDSAVYYCAGSFSNTVTFGAGTVLVLKGQDYKKYTVLQPTVPQKLHPEDSVTLQCSVLTERCSGDHSVYWFRHGSGESHPGIIYTHGNRSDECKKSSETDSPTQSCVYKLPKRNLSLSDAGTYYCAVAACGQILFGKGAELKVGKHFKFTLNISSSSFHPYYNICIYIYIYICLIYRCINKAPSHICLYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C3Y9</accession>
    <name>A0A3B4C3Y9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Actinodin3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C3Y9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006448"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003560.2">
        <property type="protein sequence ID" value="ENSPNAP00000006448.2"/>
        <property type="gene ID" value="ENSPNAG00000012457.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005067"/>
    <dbReference type="OMA" id="NVDPRWY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006448.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35234" checksum="B74977B0827CC5BA" modified="2024-11-27" version="2">LLNMIKQEDVAPVSIDAEKANGFLSSSRGRRSVDPRWYRQTPDFQAYYRYYSSIGHTEGLYEIDKIRMLYQQMRHLEHVYGPNASYYQSKLGIPVLPPLPKCDPAKDKNCKPPPPSPAPEKKPAPPVPALVKGLTPPAPISQADVVYLCNAKDPLCKPHIVYMPTGAVPVLCDPRYHPTCKLEGPPAPAPVPIVIKKVAPPPKVAPPPPPIMYKGMEYDCDPYWDPDCLIDNPPRFIKGKVAMPPPVPQPEEDEEEEEEEEASTPAPPAPVVKKHPVPYYGYPYYPYYPYDFQSELYDSRRYSYPSADTNTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5I5</accession>
    <name>A0A3B4C5I5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017575474.1">
        <property type="nucleotide sequence ID" value="XM_017719985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5I5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006978"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002625.2">
        <property type="protein sequence ID" value="ENSPNAP00000006978.2"/>
        <property type="gene ID" value="ENSPNAG00000012914.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440839"/>
    <dbReference type="CTD" id="570544"/>
    <dbReference type="GeneTree" id="ENSGT01150000286934"/>
    <dbReference type="OMA" id="RLYDYEW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022010">
        <property type="term" value="P:central nervous system myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031643">
        <property type="term" value="P:positive regulation of myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048714">
        <property type="term" value="P:positive regulation of oligodendrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001227">
        <property type="entry name" value="Zinc finger and BTB domain containing 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001344">
        <property type="entry name" value="Zinc finger protein 16 like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000761">
        <property type="entry name" value="Zinc finger protein 449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="313"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="497"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="239"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="339"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006978.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="540" mass="58858" checksum="B95E9C16C68325AD" modified="2024-11-27" version="2">MSRKRSHGFIETGQSSESQPALLEAVGSSARSEESFSELETEEELLCSVSQITEHLGRNITVVLEAALSEIRKIVSVRIRVLKVELREKTEEIELLKARLECVERDGRGELGLSGLDAAAFSRRAELLGQKYASEQKKAKTGAPVVKKENINAICDYLMKDKNARGGGAEVDSEQLSQSGAEPERSLWTDGGMGAGAAAEPGSDAGAEDLFSMLPSGSKRLYDYEWMAGVELNSADFKGESESKCENVTAAREADEEVEESEEGRGGLGLVSDTPSASFPLDTHASSGDDRSSPVGDSMDRLDPGQHFSAHTFICPFCGTLCPDSTFLEEHMKLMHRDESMQAMQSTSSASRVEGADGEAGRAAGGSEVPPVRVPRERKVEGGYECGDCGRHFNYLGNLRQHQRIHTGEKPFVCPECGERFRHAARLKSHRLSHSGAQSPFPCPQCGKGFPVLSGLKRHQRVHTGESPYACPQCGRRFKELGNLYTHMRIHSGATPYSCYQCGRSFRHLGTYKSHRCTPATQLPSGHSPAWAQEDKVQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5P0</accession>
    <name>A0A3B4C5P0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZNF7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5P0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006400"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003617.2">
        <property type="protein sequence ID" value="ENSPNAP00000006400.2"/>
        <property type="gene ID" value="ENSPNAG00000032284.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282534"/>
    <dbReference type="OMA" id="HSKSHEG"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000902">
        <property type="entry name" value="Zinc finger protein 445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002604">
        <property type="entry name" value="Zinc finger protein 715"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000286">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="244"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="497"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="581"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="609"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="636"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="664"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006400.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="701" mass="80776" checksum="DC93047DCA258DBF" modified="2024-11-27" version="2">CLNKGLCGGDGRGWWGGAHSTQKISSADLHTNSPCKPTQKTTGKKRAHSCSECGKGFTTQSHLQRHQRIHTGEEPYHCSQCGKRFSRQSNLQTHQRIHTGEKPYYCSECGKSFAVQCNLQSHQRIHTGEKSYYCSECGLSFRRCSDLRKHQRIHTGEKPYHCSDCGKRFSQQSHLQRHERIHTGEKPYHCSDCGKSFTQQSSLQKHQRIHTGEKPYHCSDCGQSFAVQSSLQTHQRIHTGEKPYYCSDCGQSFTTQSHLQRHQRRIHTGEKPCRCSECGKRFSQQSNLRKHQRVHTGEKPYQCSDCGKSFTTQNSLQQHQRIHSGEKPYHCSECEKSFSSQSNLQQHERIHTGVKPYYCSDCGKSFTTQSHLQRHQLIHTGEKLYHCSVCGKNFREIDSLKNHQRIHTGEKLYHCSECGKSFNQQCSLRKHQRIHTGEKLCHCSDCGKSFIQQSNLRKHQRIHTGEKLYHCLECGKSFNQQSNLRTHQRIHTGEKPYHCSECGKRFSQQCKLLNHQRIHTGEKPYQCSECGKRFRQQSALKTHQRIHTGEKLYHCLECGKSFRESGALKRHQRIHTGEKLYHCLECGKSFRESGSLKNHQRIHTGEKLYHCSECGQSFKSGALKTHQRIHTGEKPYQCSECGKSFSQQSSLRTHQRIHTGEKPYQCSECGKSFRASGALKKHQHIHMGEKLHHSIECGGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C9M9</accession>
    <name>A0A3B4C9M9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amyloid-beta A4 precursor protein-binding family B member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein Fe65-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">APBB3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May modulate the internalization of amyloid-beta precursor protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with APP (via intracellular domain). Interacts with APLP1 and APLP2 (via intracellular domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037401961.1">
        <property type="nucleotide sequence ID" value="XM_037546064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C9M9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007616"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001535.2">
        <property type="protein sequence ID" value="ENSPNAP00000007616.1"/>
        <property type="gene ID" value="ENSPNAG00000013376.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431352"/>
    <dbReference type="GeneTree" id="ENSGT00390000000002"/>
    <dbReference type="OMA" id="SWQEEYF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01272">
        <property type="entry name" value="PTB1_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01271">
        <property type="entry name" value="PTB2_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000003">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000153">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000143">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039576">
        <property type="entry name" value="APBB1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058:SF10">
        <property type="entry name" value="AMYLOID-BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="12">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="11">
        <location>
            <begin position="122"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="11">
        <location>
            <begin position="292"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="480" mass="53787" checksum="2BFDA4351321963E" modified="2018-12-05" version="1">MLGKDYMLAIIIVNYDDDIWNDQSLALDSDLPPGWRTIRDSSGTYYWHVPSGTTQWQHPSFSTEEEHNAIDGLTTNQQSDGADGRRESRGRLTERPMTSLSDQVSWQEEYFCTNMDPDSKCFAVRSLGWVEIPEEELAPGKSSLAVNNCIQQLSHSKAEGRDAVGAWGEGQDMMMVLKKDTLSLLDPVDRSLIHCQPIINIRVWGVGCNSGRDFAFVASDKDTCMLKCHVFRCNAPAKTIASALHEMCSKIMAEKSAMHPSMARSVTMESISPEDLPHQVNFLDAVRQRVQKFDVEYIGNLPVSRAMGMEVLNRAIESIMHSRDRDEWEPIVIHVSDTLLSLWRGEDGEDPFWECQVRYLTFLGVGHDTHTFAVIVDAGTQRFECHVFWCEPDAGIISEAVQAACMVQYQKCLVAQTPPLRSKMWRAGSKVKRANSMDGATFPPCHQGHTTPKMASSSVKKGMLAFFETFRNKPSAVPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAW1</accession>
    <name>A0A3B4CAW1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear receptor-binding factor 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAW1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009077"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015306.2">
        <property type="protein sequence ID" value="ENSPNAP00000009077.2"/>
        <property type="gene ID" value="ENSPNAG00000014632.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000275011"/>
    <dbReference type="OMA" id="AISCHGE"/>
    <dbReference type="OrthoDB" id="3694230at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039679">
        <property type="entry name" value="NRBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015056">
        <property type="entry name" value="NRBF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14964">
        <property type="entry name" value="NUCLEAR RECEPTOR BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14964:SF2">
        <property type="entry name" value="NUCLEAR RECEPTOR-BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08961">
        <property type="entry name" value="NRBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140361">
        <property type="entry name" value="MIT domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear receptor-binding factor 2 C-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009077.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="213" mass="24489" checksum="353791F6285DE019" modified="2024-11-27" version="2">MSRSTHQRHVAPQTVQELADALVQVWEEIPQETIHHLIRSMPRRCREVKLSLELQRGRHLQQQRLIRDTWKKGKLAKKPSIYTKETPLPPTQSPPATLTTSPKAIWCVAQESPQGCKAAKDDCTRLEEQSMAIADLRKALAVLLKENEKLLEENESLKVENARLKRDSYTDQQSPLLTPLLNSVEATQWPLLLDLPLLHLPPDLQEEIRLLWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CAX7</accession>
    <name>A0A3B4CAX7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATPase H+ transporting accessory protein 1 like b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vacuolar ATPase subunit S1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568187.1">
        <property type="nucleotide sequence ID" value="XM_017712698.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CAX7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008415"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000175.2">
        <property type="protein sequence ID" value="ENSPNAP00000008415.1"/>
        <property type="gene ID" value="ENSPNAG00000014011.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436292"/>
    <dbReference type="CTD" id="561786"/>
    <dbReference type="GeneTree" id="ENSGT00940000158156"/>
    <dbReference type="OMA" id="SRKGQWE"/>
    <dbReference type="OrthoDB" id="9442153at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033176">
        <property type="term" value="C:proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030641">
        <property type="term" value="P:regulation of cellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.110:FF:000003">
        <property type="entry name" value="ATPase H+ transporting accessory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008388">
        <property type="entry name" value="Ac45_acc_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046756">
        <property type="entry name" value="VAS1/VOA1_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046755">
        <property type="entry name" value="VAS1_LD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471:SF3">
        <property type="entry name" value="ATPASE, H+ TRANSPORTING, LYSOSOMAL ACCESSORY PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12471">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20520">
        <property type="entry name" value="Ac45-VOA1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05827">
        <property type="entry name" value="VAS1_LD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017379744" description="ATPase H+ transporting accessory protein 1 like b" evidence="7">
        <location>
            <begin position="24"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1 luminal" evidence="8">
        <location>
            <begin position="112"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="V-type proton ATPase subunit S1/VOA1 transmembrane" evidence="9">
        <location>
            <begin position="274"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="37626" checksum="DC0892AF14D1094A" modified="2018-12-05" version="1">MAERRLYTLFSLLLFALVQPSSPFNQVPAILDRSSEVPPYPAIRIRSDGVGVESPSASHEESYSPAAENPLRRILQPYGWHLHAQPRTKRKLLQSTSSGPYSPLNVAYNGKICILFKAKRLAIRYRNNTFLDLTERVFGPNAPVDTKGSVCTKEKATLSLRLGDVEDIKGLVIRLQMSNTFYESAGQNWFTLDSVHIHYNWTHEATFNATEVYAPATYSYHCQHVSSLQKYDTLLVPSSHTDSSANWHITFTDFQIQAFNVQSNKFASASDCATFFTPAILMGLITSLILLLVLAYALHMVVHLKHIDRYEEHKTTVYFPRTPEAELPDKNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDN7</accession>
    <name>A0A3B4CDN7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RBM41</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017559825.1">
        <property type="nucleotide sequence ID" value="XM_017704336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CDN7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010047"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016600.2">
        <property type="protein sequence ID" value="ENSPNAP00000010047.1"/>
        <property type="gene ID" value="ENSPNAG00000003395.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431292"/>
    <dbReference type="CTD" id="55285"/>
    <dbReference type="GeneTree" id="ENSGT00530000063786"/>
    <dbReference type="OMA" id="ELMALFC"/>
    <dbReference type="OrthoDB" id="277802at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030626">
        <property type="term" value="F:U12 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045164">
        <property type="entry name" value="RBM41/RNPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16105:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16105">
        <property type="entry name" value="RNA-BINDING REGION-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="346"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="446" mass="50478" checksum="84B949DA7201F0C7" modified="2018-12-05" version="1">MKRVTRQECDEGPVPEEQETEGQRQLHSLLLQQLDTDVNVDRCVAKKKCFAPAALYKPFGEQAAGVRSLAQFQALQDGEKELASLRELGLTDAEIELWQSRDLIETEWKNRGVHVASEVRDARLQVIRDKMTARVELLSRPQRFSASRALSRREMEIEKSLFHGNDRCSFLTALYHQEEDSQNGQQGATSSNPMDTLYRDFLKDQQKASDYSTLDSHSPLLAPSNKLYLTETDTCHNQSLLPDQDPDRLHAPKDCPKSSVSHEPVPPIADKAQCQNQEKHSGLKITVNQPIGRLTASSSQIQNGPVTVSGRIEEITDEEIRNNREMEEGIRKIPRFQNYQRGEPSHVLCVKNMSPRASLAQLVSLFSRFQKDDTNPILYRLLTGRLKGQAFITFSDIETAHTALDMLNGYRLLDKPLVIEFGRERKNASGDSTTTTTQVVLEDYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDU2</accession>
    <name>A0A3B4CDU2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017561664.1">
        <property type="nucleotide sequence ID" value="XM_017706175.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CDU2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015283.2">
        <property type="protein sequence ID" value="ENSPNAP00000009046.2"/>
        <property type="gene ID" value="ENSPNAG00000003207.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108432391"/>
    <dbReference type="CTD" id="561772"/>
    <dbReference type="GeneTree" id="ENSGT00940000156131"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01178">
        <property type="entry name" value="IPT_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07881">
        <property type="entry name" value="RHD-n_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF6">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="11">
        <location>
            <begin position="433"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="75"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="219"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="748"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="874"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="874"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="946"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="975"/>
            <end position="1003"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009046.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1088" mass="116662" checksum="3CC19E56DC885C7D" modified="2024-11-27" version="2">MTSANCSGNEELDFRLIFGEDGQQQPPPLGPADLEPDDNTSFYILNAAQPPSVTNQSLGIQRHGVQLHSQLTATSPPLQPLLKPFEPSYESQGSKYPPTGGGGVGGGGGGGGPPKAFECPSIQITSISPSCQQEMDVSEDEMRAKGPDGDYHDRPLSRDHLYLPLDHSYRDSSLSPSPCSSLSSRSWFSDASSCESISHVYDDVDSELNEAAARFTLGSPLTSPGCSPQGGPLEEPWHQQQPQQHHHHPAFAHSLSHPHAHSLSPRGSPCHSPRTSVTDENWLSPRPPSRPSSRPTSPCGKRRHSSADICYPGSPHHSPTPTPGPSPRGSVTEDTWIGSPSMGMSPFQCCPADTDIPSKTRKTSQDRTAVMQPGKGELVLDDSGNMSPLLDSPAEEAMHGLKKDGPGEQFLSVPSHFTWNKPKPGHTPIFRTSSLPPLDWPLPSQYGQFELKIEVHPKAHHRAHYETEGSRGAVKAASGGHPIVKLIGYNEKPVNLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIMIASTKVLEIPLLPENNMSASIDCAGILKLRNSDIELRKGETDIGRKNTRVRVVFRVHIPQPNGKVLSLQAASIPVECSQRSALELPQVEKVSVSSCPVSGGEEMVITGSNFFPESKVIFLEKGPDGRPQWEVEAKIIREKSQGLSIVVEVPPYHSKSVTSAAQVQFYVCNGKRKRSQSQRFTYVSVLVKQEHREDLEPPAVPPMPMPLAHATGLVHSQPLSSPEHSHPQDGLLSSSPRGLAPSLHPLQAPIAPMGSPTFPHLPPHLQVRGLHPAPECQMPFHPAPRASYQPLQHNLPFNGQPGLPMPGTTSPQAYERVPFQPDPAGCQPLGMGMIYGPPVSAPASSGAGGPASSPSHVACTGSNQPMAHSTPHLHSLGYHCPTPGQLPPSPTHALGQPASQLQRPLGYHPAGQRSASCPTPSSAPVRMVPSPHSGPSSPQLHSLPYQSPTSPSPTSSPLGPLSHSGQPSPQASSPVLTGLPATGPLVHPLTQQQQQGPFPAEGERLNIKQEPEDRELTFRSIGLQDITLDDVNEIIGRDMSEAPGVHGTSTAHSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEE7</accession>
    <name>A0A3B4CEE7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyrosine 3-monooxygenase</fullName>
            <ecNumber evidence="8">1.14.16.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Tyrosine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Plays an important role in the physiology of adrenergic neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tyrosine + O2 = (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin + L-dopa</text>
            <dbReference type="Rhea" id="RHEA:18201"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:57504"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="EC" id="1.14.16.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 21">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.2" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017570894.1">
        <property type="nucleotide sequence ID" value="XM_017715405.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEE7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009883"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016388.2">
        <property type="protein sequence ID" value="ENSPNAP00000009883.1"/>
        <property type="gene ID" value="ENSPNAG00000015389.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437952"/>
    <dbReference type="CTD" id="414844"/>
    <dbReference type="GeneTree" id="ENSGT00950000182885"/>
    <dbReference type="OMA" id="QKYALTI"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="UniPathway" id="UPA00747">
        <property type="reaction ID" value="UER00733"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004511">
        <property type="term" value="F:tyrosine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006585">
        <property type="term" value="P:dopamine biosynthetic process from tyrosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03345">
        <property type="entry name" value="eu_TyrOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000002">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000024">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041903">
        <property type="entry name" value="Eu_TyrOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049321">
        <property type="entry name" value="TH_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005962">
        <property type="entry name" value="Tyr_3_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01269">
        <property type="entry name" value="Tyr_3_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF38">
        <property type="entry name" value="TYROSINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21417">
        <property type="entry name" value="TH_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16 20" id="KW-0408">Iron</keyword>
    <keyword evidence="13 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="14" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="23">
        <location>
            <begin position="131"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="479" mass="54720" checksum="0752CCE8E089F35F" modified="2018-12-05" version="1">MKTDGAAQSAPFTGRKRSLIEDARRDRDRERDRASSSASPGPSLSSDSFVFEEEKSAKVTINLLFALKNEKNAGFFKAGKVFETFEAKLLHLESRPGRRSKNSPSDDLEFFMKCEVHSSDIEIFINSLKRVADDVRIAQDEKVPWFPRKISDLDKCNHLITKYDPDLDQDHPGFSDPEYRKRRAQISELAFNFKHGDPLPYVEYTPEEVATWREVYRTLTAIYSSHACRQFLEGLQQLERECGYGEDKIPQLRDVSAFLKDRTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHSSAPMHSPEPDCCHELLGHIPMLTDREFAQFSQEIGLASLGASDEDIEKLSTLYWFTVEFGLCKQNGAVKAYGAGLLSSYGELLYALSNEPEYKPFNPVETAVQPYQDQTFQPVYFVSQSFEDAKNKMRQYSSTIQKPFSIRYDPYTCCIEVLDNPCKIQNVLGQMREELKILHSALERLGQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFK9</accession>
    <name>A0A3B4CFK9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Myosin VIIAb</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000035846.2">
        <property type="protein sequence ID" value="ENSPNAP00000010752.2"/>
        <property type="gene ID" value="ENSPNAG00000016536.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155350"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17092">
        <property type="entry name" value="FERM1_F1_Myosin-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17093">
        <property type="entry name" value="FERM2_F1_Myosin-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd13198">
        <property type="entry name" value="FERM_C1_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13199">
        <property type="entry name" value="FERM_C2_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01381">
        <property type="entry name" value="MYSc_Myo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000012">
        <property type="entry name" value="Myosin VIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.530:FF:000004">
        <property type="entry name" value="Myosin VIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000007">
        <property type="entry name" value="Myosin VIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000013">
        <property type="entry name" value="Unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000075">
        <property type="entry name" value="unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000079">
        <property type="entry name" value="unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000036">
        <property type="entry name" value="Unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000051">
        <property type="entry name" value="Unconventional myosin-VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057130">
        <property type="entry name" value="Myosin_VII_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041793">
        <property type="entry name" value="MyoVII_FERM_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041794">
        <property type="entry name" value="MyoVII_FERM_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036106">
        <property type="entry name" value="MYSc_Myo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF34">
        <property type="entry name" value="MYOSIN VIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21998">
        <property type="entry name" value="FERM_C1_MyoVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24123">
        <property type="entry name" value="Myosin_VII_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8 12" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="18">
        <location>
            <begin position="65"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="17">
        <location>
            <begin position="1015"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="16">
        <location>
            <begin position="1256"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="1586"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="17">
        <location>
            <begin position="1730"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="16">
        <location>
            <begin position="1887"/>
            <end position="2190"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="14">
        <location>
            <begin position="2116"/>
            <end position="2143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="12">
        <location>
            <begin position="618"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="858"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="158"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010752.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2201" mass="253785" checksum="8A6B654E21854A98" modified="2024-11-27" version="2">MVILQQGDYVWLDPKRGHEFDVPLGAVVKLCDSGQILVVDDEGHEHWISPQNATNIKPMHPASIHGVEDMIQLGDLNEEGILRNLFIRYNESVIYTYTGSILVAVNPYQLLPIYTVDHIRLYTNKKIGEMPPHIFGIADNCYFNMQRHNKDQCCIISGESGAGKTESTKLILQFMAAISGQHSWIEQQVLEANPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAPDERNYHIFYCMLKGMSPDQKTKLGLGLATDYNYLTMGNCTVCDGRDDMKEYSSILSAMKVLMFTDTENWEISKLLAAILHMGNLRFEARTFKNLDACVVVRSPDLTKAAALLEVDHQDVMSCLTTRTLITRGESVSTPLSVDQGLDVRDAFVKGIYGRLFVWIVDKINAAIFRSPSTEDNTIRRSVGLLDIFGFENFNFNSFEQLCINFANENLQQFFVRHVFKLEQEEYNLERINWQHIEFTDNQDALDMIAIKPMNIISLIDEESKFPKGTDATMLSKLNSQHKLNINYIPSKHSHETYFGIQHFAGVVHYETKGFLEKNRDSLHSDIIQLVHSSKNKFIKQIFQADVAMGVETRKRSPTLSSQFKRSLELLMQTLSVCQPFFVRCIKPNELKKPMLFDRGLCVRQLRYSGMMETIRIRRAGYPIRYTFAEFVDRYRVLMPGVKPAHLQENLRGTCQRIVTSVLGRNDDWQIGKSKIFFKDHHDMQLEIERDKTITNKVILIQKAVRGMKDRANFLKAKRAVTLIQTMWRGYICRKNYRVMRAGFLRLQALCRSRKHYLAYRDTRTRIILLQGRCRGFLVRRTFWRRLNAIHTIQAYARGMIARRLCKRLRLEHRLEAERQRLAEEERLRNLMTARRARAEAERKHQERLAQLVREQEEREMAKREEARRKKELLVQMEQARQEPVSDSEMVDKMFGFLGNSTSLPNLEGEAPAGFEDLEQVSKDYEEDSIDEALPLPEEEQEDLSEYKFPKFAATYFQGNITHTYVRRPLKQPLLFHEDEGDQLAALAVWVTVLRFMGDLPEPKYQISVSDGSEKIPVMTKIYETLGKRTYKRELQALQGEEESSIIDSQKRNSVRHKLVSLTLKKKTKITEEVTRRLTDGDYSIQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQICKQLIQNPSKSSHARGWILLSLCVGCFSPSEKFVKYLRAFINVGPPGYAPYCEERLRRTFVNKTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNSLADKIGLKDRFGFSLYIALFDKVSSLGSGKDHVMDAVSQCEQYAKEQGAQERNAPWRLFFRKEIFTPWHNPSGDSVATNLIYQQIVRGVKFGEYRCDREEDLAELASQQYYVEYGVDILQDRLLSLIPSYIPDREISSARTMDKWAQVIIATHKKGIYTQKRFNPQRVKEEVVDFARFKWPLLFSRFYEAFKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEITAVSSSRSFLNCCTVKSLRFNLQSDLNVKGGGKLQGQSFTLATVKGDEYTFTSNNAEDIRELVVTFLDGLRKRSKFVVALVDSHNTVGQDSSFLSFSKGDLILLDEHTGEHVLTSGWAHGVNDRTKKRGAFPADSVYVLPTVTRPQYDIVALVTMSKDQRKDSISLSQLNMMESEEKVKPYTLEEFSYDHFRPPPKSTLSRVMITKGRGKDKLWCCSREPLKQPLLKKVLNHEELSQDACLAFIDILNMLYMGDYPSKRTRTVNELTDQIFEGALKAEPLKDEIFCQIMKQLTENHVKYSEEKGWELLWLCTGLFPPSNNLLPHVQKFLQSRKHHPLAPDCMQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCLNISGRMRLKSSEGFSLFVKISDKVISVPDGDFFFDFVRHLTDWIKKARPVKDGIVPSLTYQVYFMKKLWTNTVPGKDSMADTIFHYHQELPKYLRGYHKCTPEEVFQLAALIYRVKFEEDKSQFINISKMLKELVPQDQIRQLSPDDWKRSIVACFNKHAGKTREEAKLMFLKIVYKWPTFGSAFFEVKQATDPHFPEILLIAINKHGVSLIDPKTKDILMTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTTMTKQRSSRGNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFL4</accession>
    <name>A0A3B4CFL4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HNRNPD</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_037403153.1">
        <property type="nucleotide sequence ID" value="XM_037547256.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037403154.1">
        <property type="nucleotide sequence ID" value="XM_037547257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFL4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010757"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017515.2">
        <property type="protein sequence ID" value="ENSPNAP00000010757.2"/>
        <property type="gene ID" value="ENSPNAG00000016368.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440733"/>
    <dbReference type="GeneTree" id="ENSGT00940000158010"/>
    <dbReference type="OMA" id="LQDEHTI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12583">
        <property type="entry name" value="RRM2_hnRNPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000030">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000156">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein d0 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012956">
        <property type="entry name" value="CARG-binding_factor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF3">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08143">
        <property type="entry name" value="CBFNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="52"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="137"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010757.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="307" mass="34535" checksum="5A6A1F7CAF6D1F64" modified="2024-11-27" version="2">MSEDLLSADDPTMKMEEDGEGSAEDTAACGDGGPGEAEGSKIDASKNEEDEGKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPMTGRSRGFGFVLFKDAESVEKVIQQKEHKLNGKVIDPKKAKAMKSKEPVKKIFVGGLSPDTPEEKIREYFDAFGEVESIELPMENKTNKRRGFCFITFKEEEPVKKIMEKKYHNIGLSKCEIKVAMSKEQYQQQQWGGRGGYPSRSRGRGGPNQNWNQAYGNYWNQGYGNYGNYGYNNQGYGGYGGYDYSGYNNYYGYDQQSGYGKSPRRGGHQNSYKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CGW5</accession>
    <name>A0A3B4CGW5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GNG8</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017553993.1">
        <property type="nucleotide sequence ID" value="XM_017698504.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CGW5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011247"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036051.2">
        <property type="protein sequence ID" value="ENSPNAP00000011247.1"/>
        <property type="gene ID" value="ENSPNAG00000016907.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427916"/>
    <dbReference type="CTD" id="94235"/>
    <dbReference type="GeneTree" id="ENSGT01100000263497"/>
    <dbReference type="OMA" id="NIERMMI"/>
    <dbReference type="OrthoDB" id="6264244at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="70" mass="7884" checksum="C81E0B4150409FB7" modified="2018-12-05" version="1">MSNNMAKIADARKTVEQLKLEVNIDRMMVSKAAAELMSYCETHAKEDPLVTPVPSSENPFREKKIFCAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CJ40</accession>
    <name>A0A3B4CJ40_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin T, slow skeletal muscle</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Slow skeletal muscle troponin T</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TNNT1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with TPM3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552867.1">
        <property type="nucleotide sequence ID" value="XM_017697378.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CJ40"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012027"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036359.2">
        <property type="protein sequence ID" value="ENSPNAP00000012027.1"/>
        <property type="gene ID" value="ENSPNAG00000017742.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427325"/>
    <dbReference type="CTD" id="7138"/>
    <dbReference type="GeneTree" id="ENSGT00940000160609"/>
    <dbReference type="OrthoDB" id="330499at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031014">
        <property type="term" value="F:troponin T binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031444">
        <property type="term" value="P:slow-twitch skeletal muscle fiber contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000001">
        <property type="entry name" value="Troponin T, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF6">
        <property type="entry name" value="TROPONIN T, SLOW SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="268" mass="32198" checksum="066CA21D4FE5D577" modified="2018-12-05" version="1">MSDVEEEYEEQAEEGEEEQEEATGQEDEEQEYTEYQEETQEEEEERPRPKPMAPQLAPPKIPEGERVDFDDIHRKRMEKDLLELQTLIEAHFEQRKKEEEELIGLKDRIERRRSERAEQQRVRAEKERDRQTRIAEERQRKEDEEAKKRADDEAKKKKVLSNMGANFGGFLAKAEQRRGKRLTGREIKRKTLAERRSPLGIENLREDGLRQRAQEMWNWIYQLESEKFDFMDQMKRQKYEIIVLLNRISHAQKFKKGHGKGKVGGRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMG7</accession>
    <name>A0A3B4CMG7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADP-ribosylation factor-like 14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMG7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000012535"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036553.2">
        <property type="protein sequence ID" value="ENSPNAP00000012535.2"/>
        <property type="gene ID" value="ENSPNAG00000018301.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176999"/>
    <dbReference type="OMA" id="VEINAMF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012535.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6191" checksum="5ADDDA36FB433255" modified="2024-11-27" version="2">MGLKGSKLPEARVLLLGLDGAGKSTLLYKLKYNERFQTVPTVGFNVEMFDAKSDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CPY8</accession>
    <name>A0A3B4CPY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 26a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017570005.1">
        <property type="nucleotide sequence ID" value="XM_017714516.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CPY8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013423"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036915.2">
        <property type="protein sequence ID" value="ENSPNAP00000013423.1"/>
        <property type="gene ID" value="ENSPNAG00000019271.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437429"/>
    <dbReference type="CTD" id="100136874"/>
    <dbReference type="GeneTree" id="ENSGT00390000014794"/>
    <dbReference type="OMA" id="HHGTQYC"/>
    <dbReference type="OrthoDB" id="10042902at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019169">
        <property type="entry name" value="Transmembrane_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22168">
        <property type="entry name" value="TMEM26 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22168:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09772">
        <property type="entry name" value="Tmem26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="35613" checksum="784AB47E270E1476" modified="2018-12-05" version="1">MLIKFICAVVTRGLFILISLIGVWRVTRVKENNLYWFLTILYLPLVFEMILTLRRRKGKDYKWFSPSIFLFLLSIIPTIWILELHHQQNKSSSAKCGKLDSPESIKNIFNSMRNTTNGTEAYKFFKDSAKFLSSVCANDWILALHQILLILLIVGKWLMPIGEGVTRDQLSQLLLIFVGTAADILEFTSETLSDVKDNSPQLVYIILAVWTWSMLQFPFHLSVVNSKAGDECESQPDTLLNRHSTDFWAVVETLFIQDGPFLLVRLTVMIHFNIIHQMLVFFTIKNLLVVTLNVYRMWVIFHDYRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQ20</accession>
    <name>A0A3B4CQ20_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RETREG1-3/ARL6IP-like N-terminal reticulon-homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017552890.1">
        <property type="nucleotide sequence ID" value="XM_017697401.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQ20"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013450"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021032.2">
        <property type="protein sequence ID" value="ENSPNAP00000013450.1"/>
        <property type="gene ID" value="ENSPNAG00000019316.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427343"/>
    <dbReference type="CTD" id="23204"/>
    <dbReference type="GeneTree" id="ENSGT00940000154937"/>
    <dbReference type="OMA" id="WTGQKEK"/>
    <dbReference type="OrthoDB" id="6416122at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006613">
        <property type="term" value="P:cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22559">
        <property type="entry name" value="Arl6IP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052114">
        <property type="entry name" value="ER_autophagy_membrane_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057282">
        <property type="entry name" value="RETREG1-3-like_RHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952:SF0">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952">
        <property type="entry name" value="ADP-RIBOSYLATION-LIKE FACTOR 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24456">
        <property type="entry name" value="RHD_RETREG1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="RETREG1-3/ARL6IP-like N-terminal reticulon-homology" evidence="6">
        <location>
            <begin position="26"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="203" mass="23297" checksum="41A39198B788630E" modified="2018-12-05" version="1">MAEGDNKSSNQLAQETAQLEEQLQGWGEVILAGDQFLRWEKPWFPGALMGATTVLFMLIYYLDPSVLTGLSCTVMILCLADYLVPILAPRIFGSNKWTTEQQQRFHQICGNLVKTQRRVLGWWKRLFALKEEKPKMYFLSVISSLVIVAWIGQQVHNLFLTYLIVSFLLLLPGLNQHGIITKYAGMAKREINKLLKHKEKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CS73</accession>
    <name>A0A3B4CS73_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3456 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the canopy family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CS73"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013369"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020948.2">
        <property type="protein sequence ID" value="ENSPNAP00000013369.1"/>
        <property type="gene ID" value="ENSPNAG00000019217.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161119"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070121">
        <property type="term" value="P:Kupffer's vesicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030917">
        <property type="term" value="P:midbrain-hindbrain boundary development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040036">
        <property type="term" value="P:regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042415">
        <property type="entry name" value="CNPY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021852">
        <property type="entry name" value="DUF3456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13341:SF4">
        <property type="entry name" value="CANOPY FGF SIGNALING REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13341">
        <property type="entry name" value="MIR-INTERACTING SAPOSIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11938">
        <property type="entry name" value="DUF3456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3456" evidence="2">
        <location>
            <begin position="99"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="253" mass="28244" checksum="EAE53F8858A60E0F" modified="2025-10-08" version="2">MVADLQSATPLAAAPVTHPCWGRARWSWQRHRPARKWPVIFLQRSCLSCFSNTARTKTGPGYLGCRLRSSVSARSRRSRFRAEKFPLLTCRCLPSRSASACVAIADELSYSISQIDPKKTVNVGGFRLNPDGSMTEKTVPLARSETHLSELLDGVCNNMSDYALYEDPDTKEHSYKRFAPRSSDGGNFPDFKNFKFAGPEGSDSLKFACETIVEELEEDIISLFTREEQHVALKLCSEVSGHCKSSVFQHGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTU0</accession>
    <name>A0A3B4CTU0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTU0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037462.2">
        <property type="protein sequence ID" value="ENSPNAP00000014768.2"/>
        <property type="gene ID" value="ENSPNAG00000020775.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183040"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07660">
        <property type="entry name" value="BAR_Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000003">
        <property type="entry name" value="arfaptin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF3">
        <property type="entry name" value="ARFAPTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="8">
        <location>
            <begin position="174"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="281"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014768.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="394" mass="44224" checksum="34019120927A014D" modified="2024-11-27" version="2">MTDSIMSKAATMEIPINSNGDSRTLGEDDGLEQARQVQWGLDEKVGRPPGQRDLQQVMVSGPNLNETSIVSGGYGGTAEGIIPTSSIKDLRMKCRGISVAPSQSAASRIASQSDDFPGIPGSSMHHSSSSSMMAEEAARGVAVEKFDMMKKWGLNTYKCTKQLISERFGRGSRTVDLELEAQIEVLRDTKRKYESVLRLARALTNHFYSMVQTQHALGDTFADLSQKSPELRDEFGYNAETQKLLYKNGETLLGAINFFVSSINTLVNKTMEDTLMTIKMYENARLEFDAYRADLEELNMGPRDAVTMARIEAAQQQYQIHKDKYERLRSDVTIKLKFLEENKVKVMHKQLLLFHNAISAYFAGNQQQLEQTLKQFNIKLKPPGADKPSWLEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CX40</accession>
    <name>A0A3B4CX40_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_037399642.1">
        <property type="nucleotide sequence ID" value="XM_037543745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CX40"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016667"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025225.2">
        <property type="protein sequence ID" value="ENSPNAP00000016667.1"/>
        <property type="gene ID" value="ENSPNAG00000022833.2"/>
    </dbReference>
    <dbReference type="GeneID" id="119264789"/>
    <dbReference type="GeneTree" id="ENSGT00410000026495"/>
    <dbReference type="OMA" id="TAKMILM"/>
    <dbReference type="OrthoDB" id="9030481at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41693">
        <property type="entry name" value="HEME-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41693:SF1">
        <property type="entry name" value="SI:CH211-243A20.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017428658" evidence="1">
        <location>
            <begin position="26"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="191" mass="21933" checksum="D1ECC7FC1268BC14" modified="2018-12-05" version="1">MAVPMATRTVTTLLVLVTFLAQSLAISNELDYGTWNYREGAENVNVANVRSVTRVLDAWGKRIFNEIKTLLHSHPSALLPDYSRVRPLSESLNDLFREVSHLQRRITDLNHRLATLEPLLRRHGYRKEGEEGGEGAEPAPQSMRGAGYSLAKYPQRYRVRVVHPFNRVVRTRRVKVYKDGIGRVKVREDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ55</accession>
    <name>A0A3B4CZ55_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cell division cycle associated 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CDCA5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sororin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552626.1">
        <property type="nucleotide sequence ID" value="XM_017697137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ55"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016640"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038207.2">
        <property type="protein sequence ID" value="ENSPNAP00000016640.1"/>
        <property type="gene ID" value="ENSPNAG00000022811.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108427167"/>
    <dbReference type="CTD" id="113130"/>
    <dbReference type="GeneTree" id="ENSGT00390000010028"/>
    <dbReference type="OMA" id="KKVQQID"/>
    <dbReference type="OrthoDB" id="9949198at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007080">
        <property type="term" value="P:mitotic metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031536">
        <property type="term" value="P:positive regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018605">
        <property type="entry name" value="Sororin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057261">
        <property type="entry name" value="Sororin-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057337">
        <property type="entry name" value="Sororin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31092">
        <property type="entry name" value="SORORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31092:SF2">
        <property type="entry name" value="SORORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25220">
        <property type="entry name" value="Sororin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09666">
        <property type="entry name" value="Sororin_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sororin-like middle region" evidence="10">
        <location>
            <begin position="81"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Sororin C-terminal region" evidence="11">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="85"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29276" checksum="1E9A2053797B1BF1" modified="2018-12-05" version="1">MSGRTPRTPRPASTGQTSKNNSQSRPTDAGSLPRRRSARLSTNEENLPPKVEAPHVAVKRSITVRKIAPRKTQVSSEPNKENVERPSSATRKRSQVSSPPQTETSAPKPTVLSPIQASSTPPHQPREPEQDPVWSNKVRRSYSRLSMGDRSFESPKPQSASSPTRRETLFGFERLQTPEVIRKADVSRGALQASSLLCGGSFNLSAADDIVSNASEPDLNIPGVCVMKKNPRRKRVQQIKMSELDLLAAKMNAEFDEAESFELVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D159</accession>
    <name>A0A3B4D159_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D159"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017273"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038465.2">
        <property type="protein sequence ID" value="ENSPNAP00000017273.2"/>
        <property type="gene ID" value="ENSPNAG00000023502.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240385"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017273.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="98" mass="10801" checksum="AC34FA9270ADED56" modified="2024-11-27" version="2">MASSSSLLSEDQLLCSICLDVFTDPVSTPCGHILCRVCLKEGWDSSSCCQCPVCKEEFSRRPDLHLNTFISGLGVFENFECGLCFNLQGLSTLQKTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D1U1</accession>
    <name>A0A3B4D1U1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Meteorin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the meteorin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D1U1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018302"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027438.2">
        <property type="protein sequence ID" value="ENSPNAP00000018302.2"/>
        <property type="gene ID" value="ENSPNAG00000024658.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001390"/>
    <dbReference type="OMA" id="TMGFRYE"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051998">
        <property type="entry name" value="Meteorin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28593">
        <property type="entry name" value="METEORIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28593:SF4">
        <property type="entry name" value="METEORIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018302.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29563" checksum="6B516B2B2324F285" modified="2024-11-27" version="2">MHSSPSLSSCLCACAVSGLARDPLSGPVQQVSLRCSTGSVRWLFPRLALRLLLKPNVASARPAALCIKASRASRGAAVYAERAGELQLLVEDGELSEQVHCVRTDGARGAAIFLQANPQSDFRRRAVSFRYELLQEKSIGSKAACRPCSDSEILQAICTSDFVVRGSIRSVSHHPERQTSVIEVEEARVYRQRSGIFEREPIMSGHWHGHIHTPLQCHVKAGAGQFLFTGAEHFGEAWLSCAPRFKDFEELYYLARAAQHITCEFPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2R9</accession>
    <name>A0A3B4D2R9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017580464.1">
        <property type="nucleotide sequence ID" value="XM_017724975.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2R9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026812.2">
        <property type="protein sequence ID" value="ENSPNAP00000017845.1"/>
        <property type="gene ID" value="ENSPNAG00000024162.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444023"/>
    <dbReference type="GeneTree" id="ENSGT00450000040324"/>
    <dbReference type="OMA" id="MQYEIPP"/>
    <dbReference type="OrthoDB" id="8730579at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098">
        <property type="entry name" value="AGAP001331-PA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098:SF3">
        <property type="entry name" value="MYB-RELATED TRANSCRIPTION FACTOR, PARTNER OF PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="83"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="412" mass="45244" checksum="CEDA3C6F4FA4D278" modified="2018-12-05" version="1">MWPKRATAGLAAEVETLRTFWIFWTQILRINPSFRGEPPPRPSVCAPRCLTVALWEIRRAGRVRMSSSSLYLSQENLVRYKKRKANFTFSEVHILLDEVRKNRHIVVGKFNSGVPSEVKRRKWAEITERVNEIGECDREVGEIIKKWSDLKCDTKRKIAALHSGSIPHSALAQAELSPTECIVESILELDKKPWEAALPSRSRSRSTDQDGGGIDDDDDGGFLGMSSVHSSPGLGVETRTMPPPVGGATGAVEAQHDALNAADPDSRLMDSDDDHHDVLPSSSLSSVNNSYAEDDGTLTGTNIAHVSSSTNHAPSQQGLSTMAAESDSAREQLAQNASLSVQEQHTTNALLATVSRSLELLAESVQQLAETQQEFVRESLQLQRETVQVLREFATGALTLLHEKVNGKPPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D4I9</accession>
    <name>A0A3B4D4I9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D4I9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019267"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039372.2">
        <property type="protein sequence ID" value="ENSPNAP00000019267.1"/>
        <property type="gene ID" value="ENSPNAG00000025796.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168125"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256:SF65">
        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11900" checksum="10A1357FC0784503" modified="2018-12-05" version="1">LIYSLMFITVGVLGQTVEQPELSWIKSQNKRARISCIVTGLQSDNYVHWYQQKDGEALKRILYVNKGGTSPVYNDNVREAKDFTLELLGASCCLWKNESWSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D523</accession>
    <name>A0A3B4D523_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NIDO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D523"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018558"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027834.2">
        <property type="protein sequence ID" value="ENSPNAP00000018558.2"/>
        <property type="gene ID" value="ENSPNAG00000005798.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164679"/>
    <dbReference type="OMA" id="TISYNQY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052749">
        <property type="entry name" value="Alpha-tectorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160">
        <property type="entry name" value="ALPHA-TECTORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46160:SF9">
        <property type="entry name" value="PROTEIN PRY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIDO" evidence="1">
        <location>
            <begin position="89"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="1">
        <location>
            <begin position="297"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018558.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="411" mass="46072" checksum="3B4769026B3A3F82" modified="2024-11-27" version="2">MHNLFNSLTVAPPPALFYPFGSGVGDTLNPRSDDGSSPAINLQSPFTFFRRTYTKLYVNNNGHLTFDQPWDSYTPTQFNAYSGRDIIAPLWTDIDNRGAGTISYNQYSNGNVLSQATRDINQHFPQFIFTASWVFVATWDKVAYYSYSGTVTFQVVLVSDSENRSYVLMNYGSIPPSPQVWMAGYKTENNTYNFTIQASNTSSLSSTTNVGIPGRWVFRVDGTASAPSTFFFPMLPGAIQHAVTVDGGSRLIQLDYPFFYFGKPHSQLYLSMDGFLAFEPLYTDAYNPTLNKDIIAPLWTDIDSYTRGNISYEQATNGSLIELATNEMNSMFSGLNFSASWVFVGTWEKMEFEPDTGVRVTFQVVLVSDSKNRSYVLMNYGSIPPDPLPWMVRGACSSLFLLISQCFMILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8K0</accession>
    <name>A0A3B4D8K0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Frizzled-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">FZD5</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D8K0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019284"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028969.2">
        <property type="protein sequence ID" value="ENSPNAP00000019284.2"/>
        <property type="gene ID" value="ENSPNAG00000025811.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161191"/>
    <dbReference type="OMA" id="QLNCAQP"/>
    <dbReference type="OrthoDB" id="10053709at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07456">
        <property type="entry name" value="CRD_FZ5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000004">
        <property type="entry name" value="Frizzled class receptor 8a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000053">
        <property type="entry name" value="Frizzled class receptor 8a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF136">
        <property type="entry name" value="FRIZZLED-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043725028" description="Frizzled-5" evidence="20">
        <location>
            <begin position="29"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="310"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="402"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="21">
        <location>
            <begin position="30"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="22">
        <location>
            <begin position="234"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="35"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="43"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="80"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019284.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="581" mass="64880" checksum="EBB2BC5CF7A7C0BA" modified="2024-11-27" version="2">MDGYPPSSGLAFGLLALVLSRLSRLTLAASKDIVCEPITVPMCKGIGYNLTYMPNQFNHDTQEEVGLEVHQFWPLVRIRCSPDLLFFLCSMYTPICLPDYKKPLPPCRSVCERAKRGCSPLMIQYGFEWPERMSCEQLPVLGDPDRLCMDQNSSETTTLSPSFPKPTPKGSRHRAMSKSAVPQKCDRECRCREPLVPIKKESHPLYNRVQTGSQPNCALPCHQPYFSQDERAFTAFWIGLWSVLCFVSTLTTVATFLIDMERFKYPERPIIFLSACYLFVSLGYIIRLLAGHERVACGGLGDQRHILYDTTGPALCTLVFLLIYFFGMASSIWWVVLSFTWFLAAGMKWGNEAIAGYSQYFHLAAWLVPSVKSIAVLALSSVDGDPVAGICYVGNQSLESLRGFVLAPLVVYLFSGSLFLLAGFVSLFRIRSVIKQGGTKTDKLEKLMIRIGLFTVLYTVPATIVVACLVYEQHYRPEWERALGCSCATERQQLGLGPDYAVFMLKYFMCLVVGITSGVWIWSGKTLESWRRFVARCFPCRARKPPPSASSMYSEASTALTARAGSAPPGSYHKPAPPSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D8U3</accession>
    <name>A0A3B4D8U3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-dimethyl-L-lysine(36) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:42032"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000019374"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039444.2">
        <property type="protein sequence ID" value="ENSPNAP00000019374.1"/>
        <property type="gene ID" value="ENSPNAG00000025937.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155484"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21784">
        <property type="entry name" value="CTD_KDM2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22181">
        <property type="entry name" value="F-box_FBXL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15555">
        <property type="entry name" value="PHD_KDM2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000005">
        <property type="entry name" value="lysine-specific demethylase 2A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041070">
        <property type="entry name" value="JHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17811">
        <property type="entry name" value="JHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16866">
        <property type="entry name" value="PHD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjC" evidence="23">
        <location>
            <begin position="208"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="22">
        <location>
            <begin position="588"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="21">
        <location>
            <begin position="643"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="430"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="709"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="827"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="430"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="709"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="720"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="846"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="876"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="910"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="994"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1044"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1059"/>
            <end position="1068"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1345" mass="152285" checksum="150C9B109808C7FF" modified="2018-12-05" version="1">MDELLYSCLFKYLVAASRTTHILLCYRSCTNECTANGYRWWHFVVSSRGCSLITFETRVGPAMEESQPRYSKRLRTGTRRRYQDDGISDDEIEGKRTFDIEEKLQSSFFNSDLVKIMDGEDFTFEYIQREGLRDPIIFPKTDGLGIQMPDEGFSVSDVKLFVGSRRMIDVMDVNTQKGIEMSMSQWRRYYETPPSEREKLYNVISLEFSHTKLEHLVKRPASVDMIDWVDNMWPRHLKERQRDSTNAIIDMQYPKVQKYCLMSVKGCFTDFHIDFGGTSVWYHILRGAKVFWLIPPTPQNLELYENWVLSGKQGDIFLGDKATTCQRIELKQGYTFMIPSGWIHAVYTPEDTLVFGGNFLHSFNIPMQLNIYNIEDRTRVPSKFRYPFYYEMCWYVLERYLYCLTNTSHLTPEFQKHSLGIGLKKEDYINPNKLDGHTEDKQKEEEGEGQEEEENDVNKDIKEENEEEAPSPPACPGVKVHLTPLELEGLWQLLHKLEELPAHKKCVPTGIRNAPALLSDIRAVLEEHTNDDPKLSYTGKPIVTWPKRPSWYRPPTPPTSLLYRSRAPGSGPMLAPVPRPVKPPSSISALRRRRVRCKRCVACLRAECGDCTYCRDMRKFGGPGRLKKTCILRQCLAPGLPLSAVCAICKEGNQEAEGSDATQTLMECSECAQITHPECIKVPGEGVINKDLPSCWECPKCVQEKNRESSSSSSEKEAESSVTTGSPSSGPPVKRAYREGIGVGGLRWGLRKGRPPLSPSSLPLTRSRRQPPPSQRLLLQHQQSRKRVSVLELQHRKRIKLERNKILQSLLRQRSLERAGLVRAALSRARSHGRGLPACRGRGVRLRGGGRGGALRGRTEREEGGADSNEEEGQEKEERRENGWGGKENRPQRHGGRANEEENGEGPQNGERKRERDEAAEHVQSDSSVVLGDDVRGQRSCVTVTLRPSKGRRDPSTIVPKLEANVSPRTLPQSPRELYGKSLLRPPLRNGVRSSDKANLHTESSHTQAVRSHTDRTDLHSPTLTRGTSKHSHMGRNLRCNSGESHHIGKKPRVDRAKPPTSSSLSRPKSSEQNGASEPGWERLVWVSVFRYLTRTELCVCMAVCKNWYKWGCDKRLWTRISLSRCQSISPQALSGIIKRQPVTLDLSWANISKKQLTWLVNRLPGLKDLVLSGCSWVSISALSSPSCPLLRSLDLRWADGVKDAQIRELLSPPGSNNRSQLRNMQCLRLCGLDVTEATLRLIIRHMPQLTRLELSHCPLTDNALNLLTAVGSSTRNTLTHLNLGGCSRLTDHCLPYLQRLSCLSVLDLRGCKGVSRQACENFISELSVTALYCLSDDKLIQRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DDX0</accession>
    <name>A0A3B4DDX0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase Hakai</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Casitas B-lineage lymphoma-transforming sequence-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase Hakai</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CBLL1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the Hakai family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017572338.1">
        <property type="nucleotide sequence ID" value="XM_017716849.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DDX0"/>
    <dbReference type="SMR" id="A0A3B4DDX0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000032190.2">
        <property type="protein sequence ID" value="ENSPNAP00000021144.1"/>
        <property type="gene ID" value="ENSPNAG00000004310.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438797"/>
    <dbReference type="CTD" id="79872"/>
    <dbReference type="GeneTree" id="ENSGT00510000047522"/>
    <dbReference type="OMA" id="HLPPKQH"/>
    <dbReference type="OrthoDB" id="547746at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16508">
        <property type="entry name" value="RING-HC_HAKAI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000140">
        <property type="entry name" value="E3 ubiquitin-protein ligase Hakai isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2210:FF:000001">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase hakai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040380">
        <property type="entry name" value="HAKAI-like_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040383">
        <property type="entry name" value="HAKAI/CBLL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041042">
        <property type="entry name" value="Znf_Hakai"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13480:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HAKAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13480">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18408">
        <property type="entry name" value="zf_Hakai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Hakai C2H2 zinc finger" evidence="18">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="171"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="272"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Pro residues" evidence="17">
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            <begin position="379"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="465" mass="51288" checksum="BAA369185402EF5B" modified="2018-12-05" version="1">MDPIDSDMQGTEGALGGPDVRRRIPIKLLPKQARKGFAYKQEERFECGAKAGDAFAGQRRFPQQFYWDYKLNLIGEKDETPVHFCDKCGLPIKTYGRMIPCKHVFCYECALLHERKGDKMCPGLTLYSCTDPVQRIEQCQRGSLFMCSIVQGCKRTYLSQRDLQAHINHRHMRASKTSSGRVDALHLPSSSEVSDRFRVPPPHLPKPHVLIPPPLPHAGHDTYSQPPPASHDDHRAPQGQPAADMGPPRSLAPETFRIATVTTRKHSNLITVPIQDDSTSPAPRDQLPQPVNAAPPHHHPGDYPGQPVVSHPHHMMAPPQQRYGPPPPPPPITHPMQHPAQGSGTHMVFNQAPPPPLSSVPPPITPPPGHLMGQLPPYMNHPPPGPPPQHGGPPVNAPPPHHYNPQFPEDKGTLSPPFNQPGGLSPGMWPAPRGPPPPRMQGPPPQGQMPGPHHPDQGRYRPYYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DE21</accession>
    <name>A0A3B4DE21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenosine deaminase RNA specific B2 (inactive)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ADARB2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017560487.1">
        <property type="nucleotide sequence ID" value="XM_017704998.2"/>
    </dbReference>
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    <dbReference type="STRING" id="42514.ENSPNAP00000022667"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034904.2">
        <property type="protein sequence ID" value="ENSPNAP00000022667.1"/>
        <property type="gene ID" value="ENSPNAG00000030072.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431678"/>
    <dbReference type="CTD" id="105"/>
    <dbReference type="GeneTree" id="ENSGT00940000157252"/>
    <dbReference type="OrthoDB" id="10268011at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="DSRM_RED2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenosine deaminase RNA-specific B2 (inactive)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="A_deamin"/>
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    <dbReference type="InterPro" id="IPR044460">
        <property type="entry name" value="ADAR3_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF17">
        <property type="entry name" value="DOUBLE-STRANDED RNA-SPECIFIC EDITASE B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
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    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="130"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="289"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="A to I editase" evidence="4">
        <location>
            <begin position="410"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
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        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="743" mass="82814" checksum="225CD8D00EDEB61A" modified="2018-12-05" version="1">MASVLASSSPGGAVRCKFKRRRRRRAKRKEKVSSMLSALIVPFKYMSPGPGSTEDEDTLSTSSAEVKENRNFGNLEDCTIVPSDRQPLFLSDSSRGSTSSLKRKRPLEEDNNGRPCRLRLCYRKLPWSDAPKNALVQLNELRPGLQYRTVSQTGPVHAPLFSIAVEVNGLTFEGTGPTKKKAKMRAAELALRSFVQFPNASQAHLAMGGLSDPSADFTSDQADFPETLFKGFEPDGCRSAESELLSSALRLRHTLDLMVVQARQGNPAPEAPPPSPVVLLNNLRPGLRYACLSQSNQGRGRSFIMAVRVDGRIFEGSGRSKKLAKRRAALSALQALFNIRQTPEARAAHRPSRIKCPHLPQDFADAVFHLVREKYSELIGCSSLAHTCQKGLAGIVMTRGLDVRQAEVVALSTGTKCINGEYMSDQGLVVNDCHAEVTARRALLRFLYSHLELHLSKRQDEQQHSIFIQHRSSFKLRENILFHMFISTSPCGDARINSPYELTNDFSVHSGRHLVKKFHSHLRTKIESGEGTLPVRCRAPVQTWDGVLQGERLITMSCTDKIARWNVLGLQGALLSHFVEPVYLTSLTVGSLRHTGHLSRTLNQRLERLGPLPPCYRRNQPLLSSLSSSDWWQQAKAPCYSVNWTAGDDQLEVINASTGKRRDCGAPSRLCKHALFTRWTRLYRKLVVCAPGSRDVPLMYCEAKQAAGTYQTAKEQWVRALQENGLGTWVRKPPEQEQFLLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DG50</accession>
    <name>A0A3B4DG50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Neuroepithelial cell transforming 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NET1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017567681.1">
        <property type="nucleotide sequence ID" value="XM_017712192.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DG50"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000022418"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034595.2">
        <property type="protein sequence ID" value="ENSPNAP00000022418.1"/>
        <property type="gene ID" value="ENSPNAG00000029793.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436002"/>
    <dbReference type="CTD" id="10276"/>
    <dbReference type="GeneTree" id="ENSGT00940000155849"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rho guanine nucleotide exchange factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000010">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006:SF4">
        <property type="entry name" value="NEUROEPITHELIAL CELL-TRANSFORMING GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="6">
        <location>
            <begin position="178"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="390"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="533"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="67719" checksum="900BBD66CA079830" modified="2018-12-05" version="1">MEETDEPQTTTLPECTNERPKRKASWRRKSAVADAEPESPGSNDRRRPPLRRGSSFTFRTPGPQWDFSLKRKRREKDDDAVSLCSFDFKEPSTKRVRPLGRVTSLANLISPVKNGAVRRFGQTLQASFRGDGRSPSVPQHKACSKAAAPTPPKRRNSTLWSETLDVHQKGTFSTKEIKRQEAIFELTRGEQDLIEDLQLARKAYHDPMLKLSIMSEEELTAIFGDLDAYIPLHEDLLAQLAKATGPDGTVGEIGEIVVNWLPRLNAYRAYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLREILRHTPPEHPDTTSLEQAVSIIQGVLADINMKKGESECQYYIDKLEYLDDRQRDPRIEQCKSLLCHGELRNKSGTKLHVFLFTEVLVLTRPVTRNDRHCFQVYRQPIPVQDLVVEDLQDGDVRMGGSFRGAFSNADKAKNIFRVRFQDPAQGQSHTLQVNDIFHKQQWLNCLRSAMSVIQSEASAASPDVRSKRRSSTVSAVVHMEETDENQPQSAAPSSPESDEPPSPTPSATSTLSTSSTSSSNSTSPPRKSKKDKRVLCSLGKRKETMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DI85</accession>
    <name>A0A3B4DI85_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PPM1H</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DI85"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013285.2">
        <property type="protein sequence ID" value="ENSPNAP00000023195.2"/>
        <property type="gene ID" value="ENSPNAG00000007731.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165017"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF838">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="115"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023195.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="512" mass="56262" checksum="B5B22F6778D02956" modified="2024-11-27" version="2">MLTRVKTAVASFVGGIMAGAPDGEQLSGAELPHKFPYMRPAFLGLSPDEIECSADHIARPILILKETKRLPWATGYAEVINAGKSALNEDQACCEVIVVKKRPGGSSTPSKTPTAKRRSSLPNGEGLGLRENSESEGLVFHYWALFDGHAGSGAAVMASRLLQQHIIEQLQDVLDVLRNLASLPPTVLGEEPNHQATPGSNRALTRAASLRGAAGAPGSPCTPPPRFFTEKKIQHESLVIGALENAFKEMDAQIEREKAVYSISGGCTALVVAFVLGKLYVANAGDSRAIIIRNGEIIPVSSEFTPESERQRLQFLGYMHPHLLGGEFTHLEFPRRVQRNEVGKKMLYRDFTMTGWAYKTIEDEDLRFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSCCPEVKVYNLTQYEHGADDVLVMGTDGLWDVLSNEEVAESVTSFLANCDPDDLHRYTMAAQDLVMRARGVLRDRGWRITNDRLGSGDDISVYIIPLMYGNRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJ49</accession>
    <name>A0A3B4DJ49_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Adhesion G protein-coupled receptor E3-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DJ49"/>
    <dbReference type="Ensembl" id="ENSPNAT00000013875.2">
        <property type="protein sequence ID" value="ENSPNAP00000022954.1"/>
        <property type="gene ID" value="ENSPNAG00000007504.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163334"/>
    <dbReference type="OMA" id="CFILTIN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000054">
        <property type="entry name" value="Adhesion G protein-coupled receptor E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF469">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="325"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="14">
        <location>
            <begin position="2"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="170"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
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    <sequence length="454" mass="51773" checksum="F1391E907A6A931D" modified="2018-12-05" version="1">MQMVNISLNDLEIRVFAIGPRAFLKENPMLSINNTQMDIDLIGISQNNNGSAAVAFMSYTSMVDILSPKLFNPTKKTEKIMMSTVLSATLPKTTNTQLPTPVNFTIKHIEDLPPNSNLFCVYWNHSEWIVDGCYLLQTNISHSVCSCFHLSTFALIVQVNPPEKDSDPVMDLINIIAVAVGLLFLCLTLLTFALCQWNPRVTNTALTNLCISLLLAHLLFLLTQTFLKYIQPRRLLCAVLAGVLHFCFLSAFVWMFIQAVMLFVSVKNLKKIRSKQKEVLSWKYLIVIGYSIPLMIVGVSVGLFSNGYGSEKCWLKADKDFLWSFLGPVCFILAANTIVFISIMVIIISILKTMKHETLKIKQAKSDKQYIKRVMLKTVIQFYIIGCSWILGFFTDYNKVLEVVFLFFNSQQGTFIFFIHCALNQEVRQLYMKWWQSFSAACRPHTTKTWIVQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DJP9</accession>
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                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
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                <person name="Winkler S."/>
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                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
    <sequence length="2828" mass="307731" checksum="7BFDA413C6A1BD5B" modified="2018-12-05" version="1">MEGREFAAPAHLLSERGALVHRAASRISSTGHGSVQHAGPFPPGKYYPSHLPMAAHSGSGLMGNSSASFMGTFLASSLGSPPSHPPHPSRPPSSPSSPPYRGGPHSSASQIWFPHSHEAAPGYPRFSGSLAHTFLPISHLDHHANSGVLYGQHRFYDTQKENFYLRSLPSQPPLISANHGIPPMSRTGPGHPQGSCSRDTGSGMNLHKGLKEASIDRGAVSGTKDKDRPSSKQESKDRHPHSHHPQPQHHHPHQPQHHSQHPITMEDVNGRSLERHKASVLMEYKDHTQSMNKPLSACLLNGKIQNGDSGAGTGAGAGSKGSMVSCGAEGMGRGAGGTANAQVRHMGNTNTGRCTKEAASGEMRISEQPHSDCLERGQALHQSLSYSVPPPLPMSSATGGGHPTSFHCLQLHPSHPHHPHHTHHPHHSHHTHHHPDFFCPPPAPITNPSLHEKGIGNSGSREPKVTGPTFVPSVGHLGEKVGGPFQLGNPECQGVGGGGNGSKDKSMEKAGAGGGHPGSWHRKQQEQQQQQQQQQAPPQKQHPYRKAEKAPDWMHSNHHHLQQQQQQQQQGHAQQHQAVRSRSADCINSMDPDVYRPALAQEPKTSHQLSHPSNVNPQPYRDCSHTGPPPNSSPLAGKTMAQQGPGGGGGSCSMQRDGQKVARIRHQQHGRAGSDAELNQTSGTQEMKRKMEMSPYGYSNSGQHHSHQQPGVPPWAMHPHRIHPEEDQRKAYMESVSGVANGRQQPQQHQLAGMGPPPPLPALPAPAQTQQEALDSQGEGSAMKNLLKYSNQQQPLLLSQKSPFGGLGNLKTGNNSSNCTLQTNKPALPSRKGPSNENERSDCGGRSRDLGEAPHVEGEVRQPPVGIAVAVARQREPTCRPPDNHPSSRQGRAHSAMKGAPRSMYPSDSPGDEERKRMSGEHIGHPCLDRERDTFIRDNKERVEFARIHPSNSCHGDLTSHIMVPGGNSLQSGQLGDPTAHTHSTHHHWMPRTGSPSLWMTGHSYGISHTALHQNLPPGFSAAMAAPLQPVLPLPQDPSAQLVVLPSEPASHPGTHHLDVMEQPGLWPPVYRARGPPSHMQHPAVYSRSQFLRQQELYALQQHQQLQHQQHPSHQQPPQQHRVAHSIEQQHRNNHNQMQKKPEEQSMELEDILSEPRTPKPAKSYGYGASNQTSSPPRASAVHLSPCCQSPCMHPKSTASTPCPAASPAAAAPQSPAISPAPSQLPKAPEPQDKRAEGQPPQDYPQSMEPDLPPEYTYPAITMGYRGGPSPQDVRLAEPAELETIPLEPVEHAPAPLSSLEEGLECHAIVRPIPESQLPMEEKNEEDIVEEHAGGVLQQREEPDPVEAVTCCVPGDEEQVQEQATLLICPPAEASACEAAHCPITQTEEQVHEDDPIMNCQEEMAEVLPVQDDDDGVPELEAQRAQRTPDPEHPCVPDIPAEQQPNRTTDVVGEEIAEEAQVEDCVQILSTLPSPVGATLCPQTIVPSYWSLELLIAAAFCGDCPPPSPPPSTPPQGCSGQFYPSTHGMALLSELADLELQQNRHSAGNSEGEEQLIFDLQSLATLAAARALELGSKENSLAEAEKQYPAKRILNLRRKCNWTPRHEPVCPVKGAMETMDGQELAMRVRLAELQRRYKEKQKELAKLQRKHDHQKEETPRSPARRGPGRPRKRKSTPGLGPVAPPEPQKKVKSAGAVLSLLADESGGGGGGGSGDSAKKRKLSNRGFGRLGAAQMKMQCCQKSSLHEHHVGQLKPKPSGNRAAVRDRDNSACSSAAAHIYTSQRSPKTESRLPSTAANESEGQSDTGSGDSGAQDSWKGRGEMMMLRKGKRKNAGALNRASARLQQPRARILRARGQEVIDDGETSALDSESSEQEEEEEEGSENSDEGRTVKSQPSRELPSSNIFKDPSPSSVVKLEANQKAKNKRERQELYGSQLHSGTEGEVKVKKKTPCRPVPVTVAKPPGVRRKPGRPRLQEKPWTGHYRKPTGLLSFSTTSERLRRATRKSSMLRGAVSKRKGCWSGVAQSPQSDDNDCKRRTRFRQSKGRAVSRLLESFAADDGFRLGEDSSFSDEDEGGNSSSHSRNPPAPPNCVLTKELLVDGLKVLICKEDELLYAARLRTLDVPDIYSIVIDGERGNRPRIYSLEQLLQEAVLDVRPETEAVLTEGTRVCAYWSERSRCLYPGYVRRGGASEEGKPGGVMVEFDDGDRGRISLHNIRLLPPGYQVQCAEPSPVLVSSGRSDRVFSAQDERNSQMNKTSEKAASREEARAQEKPARRPGRPKGSGLKRFISDSIAKNSTSPLTWPSVDAPRKRAPVNLFQLNGSAPKKALRNKEADMFPHAPVSMTTPAKGIFSSSSFEVDSFSSIANGYSSFCSQASGTPLGGRSSPYGQKRRSDDPDAPRGRKNEFLVKLDHEGVTSPKNKSSKALLLLGGSGFGSKSIGAPPRPEAYSHPVLLVKDSRKGDSSRAELLPIQRKPSPTLRMGEYGDLGLSCHRDCHSSYSDEEEEEEDRRRRRGGGSLLPSASSGMRTAGRFLSRLSVSSSSSGSSSSSSSGSLSSSSLCSSDNDSSYSSEDEESSTLLLQSCLTPHHSLLQPPKPPTGPPQHSFVAKAMAVSNAKGGNANHSAGNKPLKRKECPSSPSKPSKDLIKRQKLLPDQGRPKVSSFLPARQLWRWSGSPTQRRGLKGKARKLFYKAIVRGRDMVRVGDCAVFLSAGRPHLPYIGRIESFWESWTSSMVVKVKWFYHPEETKLGKRHSDGKHALYQSCHEDENDVQTISHKCQVVSREEYERLSRNRKPGSSLQDLYYLAGTYDPTSGQLLTADGVSILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQA4</accession>
    <name>A0A3B4DQA4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DQA4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007139.2">
        <property type="protein sequence ID" value="ENSPNAP00000025630.2"/>
        <property type="gene ID" value="ENSPNAG00000037163.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000069547"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF42">
        <property type="entry name" value="CYTOKINE RECEPTOR COMMON SUBUNIT GAMMA ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="109"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025630.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="206" mass="23941" checksum="32F04691F8A6E9AD" modified="2024-11-27" version="2">MRRTIRCVRFLLTTVSEPTESDLKCFNDYDAVMKCHMISDKLHCPAYKLSVFHEINRTYSCSFEHFPPKTCECKFQVPGFVLTETFSATLYRGEKFVFTKTIKTGESIKPKPPTIVSVNLTENGNFLITWDTNYNEPFSSSLSTKLTYGIKGSKVNVSEHHFTKILSRLTLQPNSDYVVRVRTEFNNNSIFSDYSQPYEFRTRKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DR24</accession>
    <name>A0A3B4DR24_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/arginine-rich splicing factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Splicing component, 35 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Splicing factor SC35</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Splicing factor, arginine/serine-rich 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558099.1">
        <property type="nucleotide sequence ID" value="XM_017702610.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017558113.1">
        <property type="nucleotide sequence ID" value="XM_017702624.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017558119.1">
        <property type="nucleotide sequence ID" value="XM_017702630.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DR24"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025409"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007692.2">
        <property type="protein sequence ID" value="ENSPNAP00000025409.1"/>
        <property type="gene ID" value="ENSPNAG00000010477.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430244"/>
    <dbReference type="CTD" id="406691"/>
    <dbReference type="GeneTree" id="ENSGT00940000154883"/>
    <dbReference type="OMA" id="PLIRCDV"/>
    <dbReference type="OrthoDB" id="8093034at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12311">
        <property type="entry name" value="RRM_SRSF2_SRSF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000504">
        <property type="entry name" value="Serine/arginine-rich splicing factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051106">
        <property type="entry name" value="RNA-bind/splicing_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48028">
        <property type="entry name" value="GLYCINE-RICH RNA-BINDING PROTEIN RZ1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48028:SF4">
        <property type="entry name" value="SC35-LIKE SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="14"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="91"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="115"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="220" mass="25566" checksum="90CD35DF7CDEC4BD" modified="2018-12-05" version="1">MSYGRPPPDVEGMTSLKVDNLTYRTSPETLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAILDGRELRVQMARYGRPPDSLYGRRGGPPRRYGGYGRRSRSRSPRRRRHSRSRSRSRSRSRSRSRYSRSRSRSRSYSRSRSRSKSRTPRRSKSKSASRSRSRSKSKSRSRSRSPRSNRGSKSRSRSRSRAKSPEGNGPAQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DUY4</accession>
    <name>A0A3B4DUY4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DUY4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026891"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003970.2">
        <property type="protein sequence ID" value="ENSPNAP00000026891.2"/>
        <property type="gene ID" value="ENSPNAG00000012299.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="38"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="143"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026891.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="312" mass="34258" checksum="FDC7371561B4B09E" modified="2024-11-27" version="2">YCTSICFSTLMKCLFKVLRATVNEDIISLERLKFIHLGDKINLTCSFSSTDASAAAWFKETRGEKPLLFATAFRSTPVAFHNGFNKNGRYRAIRERSSFILNILDAEPSDSATYYCAVVDYSNLGLGACTVLVLKGIFSVLQPPVSDPVELGGDTTLQCSILTDASAGDHSVYWFRHGSGESHPGIIYTHGNRSDECKKSSETDSPTQSCVYKLPKRNLSLSDAGTYYCAVLMCGEIIFGSGTKLDFEGKSRYFQFPKSRQRVLGLPRGLLPGGLACDTSQGRSSGSTPNSFQPLVFAISFFRSLPKAHDHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DVH0</accession>
    <name>A0A3B4DVH0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 46</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CFAP46</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="42514.ENSPNAP00000027071"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003509.2">
        <property type="protein sequence ID" value="ENSPNAP00000027071.2"/>
        <property type="gene ID" value="ENSPNAG00000012504.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00570000079216"/>
    <dbReference type="OMA" id="EEFWYNS"/>
    <dbReference type="OrthoDB" id="68437at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039586">
        <property type="entry name" value="CFAP46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057466">
        <property type="entry name" value="CFAP46_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15977">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15977:SF15">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25439">
        <property type="entry name" value="TPR_CFAP46_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="810"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1358"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2256"/>
            <end position="2279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2330"/>
            <end position="2355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2261"/>
            <end position="2272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027071.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2603" mass="289846" checksum="D5E716A23786F1A5" modified="2024-11-27" version="2">MDLEIRHCLIKTQGKNDPVALKKAYAAIKDVVSGKTTSDAKCLLPELCVLCAEQALRLGCKEITEDCLMMYFESKPLPNQFLCRAYFCQAQLNSSSSVCTVEDMDKAVMYYLKAIEISKDNPRYHFLVFNASLLYFQTVRVFLRPGQRRHLVSSLTQVLSALEVVHEPDYAWRAELMLLLVECLVDAGKYKEAATFAKVTSDFIELHKPEMYPRIFSIQVRYNLIDASKTLKKTSPKLLVIYKMQKLKHMAEVSEGQNKSTELMEIFLLLTQSSQPQSPASHRSSHDLDESSPIPLADRTELLLELAFVSLQLKEHKIAEDCLKELEATDAATIAQRIMKECVECELALKKLNGHIEDYTKSSVEARLAIVGRLDTLLQRAIKEGDGQVTQCVCASQWNSCLPLLQTNLRRSIKRPLLALAHALEDINSMLLDMRCQVHAELAAIEEEEQRLEPALKHLHTALGLDEKGQYQQHLSSSLHLLQLHTSIYTTPTRPEDQAAMLIQQAKEGSGCEPAKKWHPMLITAGIALAPDSFQMVLDADSAVKVSGSSPQGQGHLEQLAAKAQQHMTCVQRVEGQLASLERGTDDRERMRLWASLVKAARKQELWDVCRTACRFCLLYDDGRWKNKRELMNEIKPVEGKHSDHGVAAVGLNQGGERDLLRLLSEVRFICAEATIHKLRSEGVELNGSPVLPIEGVARPPEDDHHWALYSDWIRELSAYATSNFLRGAELGAEIQEEWVVVNAAVYLWNYNSQLLATRGHGTLLATFSRLVELLKQTGHAGEVVLLVFLCDAVAKGIIQPWYETSGQIKQEQDSGKGGMQQQADKAKKGGGKISEKSGSTHFLTLEAATVQDIKKALEMCDYALRLCNGNEETVPIMVRKQLISTWVKTKRLLQQQIGQKLDTDDESKNEAVAAMSRVLVGVEMLLCNTNPRLMEFTVPSLGTLAQMASDCKWSDPVVELYVWFQLAHFAHQSQDHDLLMTCTQNALQLEKTAIHKAKVTACSLYSVRTVKEMLSSSACLRGLSMLHKSVGNSASYTAALGMLQSSISFAEQAGSWSLCGTAAKHYWNACLPLLTSPEQRRQLKEPLEHIVKALTNTYPRTGTEKREKETQTEPLTSALDTDNKAEDDLAVRAAMYNVLFHIYTDSGSWKSGLKILDRAIMEMPHSPQRLLLYKQRVLAKAKLGESIVLDMQRFKEEGEQACALMWHRVALCAKDTQQQLACYQNAISTLKSASSQWQKVDFLLEFGEWLYYSNFPVVDAQLQIQSAIDILLTATPNTSQSPETSGRPEGVIPVSYLSELREVWRLDGLVRAHTLLAFMESRVSPLHQQYMLLAYSSVLQIWQVSMESAQEVIKEERKNPAVIAPPTSAASTKKDKEKEKEKEKGKKSKESPLVEKRLRGSVSDIFLPSSPEEWAHFECPEEVRHAFRHDSGPYSINTHSIRVQSRTLFYLDVLVKELESISLTPLTLVPLHLAEVIAHDLSLSKSHSDLYRLRIVKTCCDLGLESSSPFRESLLSFACVPEDEQMVCRNAIALQRERNGYESESKIKTDELLPPHSGSTAAVDSVSRKRLGWCTVQELWLDKAALCLSMGLYQSARSLLAEAHLVAKGLGEKMSFARSYHLLAVLASQEQQHGQALALLKQAEEIGGDEDFWFHLTQSLLNTTANCAGEDMYIQVCQIVDRAISLLRSALEQRPNRAPVLHFYIASLQAKGAVLCRSTLRPACADRVKTVERLKSICDTLKHSAAALLQHGYRTPAAETTLEQANSLRMLAMLTSVKEEKQQHLLDALVLMQQAVSLQEEVLSHCLKLLPTECGRYSLPAVRVCVDFRLALVNLALLMLEMQCEEELLKVRLRSWKSSIERAVEDYILSNTDLNALEQDWLTVTQSLGQMALTQLSVINSLSLDCIETKARSIAMLGRCLRLIALQRDPLCTIPLWDGPVMVENREERKANEGDDDDEEEACMGRVDGSTIQNGESTAKSVELQTNRRAVQQKLAQASETLAQALSLALQHNFPHVLSQVCVDLIECYSQYDPAVCGQYLALLQSCVCCADMSSILHAACSDPGHSQLAALLGLQKTLQSSPQVLLSAIDHSLHSLSKGYQHLTINPNHLSLLGEMPPNLKILLLQHSQDGSVLYGAFYEKTKATESQKGKSMQIAAGGLACSRVAKARVHPSVLHQLQDQVQAFRHLAAQALLADSRNHICAIPEHCTQLDDSTDRELGLHLQAIVKEMNDYLDPVLSQFDFSCFSQRPPSISIGESARSKDKEERGTADKALPVGSPAEQGECVVLLADRMLLEMPLEALAVLQGDGISSVSRDFSLQVFHTRLQREQPVESDHKKETKGGKPAKGKGDQSKAIKVVPVNRILPLNTLPVDTHNFKYVVDPQNDGGDCEWSSPAERMRKTLEMYSQQFTPLWDGIIGSEHVCSPADLEPLLANCSSFIFYGTERFLSHISPARLVTLNLTECQMAILFDLVQNNTSMLRQSQQDVQKSKALLALEAPLQCVYLLTLCGVHSVMLNQWSSSAKTNAQNMEAIMENLLKKGLLSGQSVHTLRRSASVTERDVNALGQRVAMCPIDVDSVLGCGSPSSLNFIIYGLPNLVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DW07</accession>
    <name>A0A3B4DW07_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017565807.2">
        <property type="nucleotide sequence ID" value="XM_017710318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DW07"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027159"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003296.2">
        <property type="protein sequence ID" value="ENSPNAP00000027159.2"/>
        <property type="gene ID" value="ENSPNAG00000002308.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434883"/>
    <dbReference type="CTD" id="80222"/>
    <dbReference type="GeneTree" id="ENSGT00940000159348"/>
    <dbReference type="OMA" id="PILEQTC"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF42">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="14">
        <location>
            <begin position="56"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="315"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027159.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="688" mass="77753" checksum="9D61545FD5B4FAC6" modified="2024-11-27" version="2">MVSALRARWLLQPGPFGSAWRSCRELHPSAGQLERLRLFETLKVRKSEQTKAAPGRDLSVRLADGKTVKALSGVTSPLQIARNIRVKGAVVGRVNGELWELARPLEADCELQLLGFDSLEGRRVVWQTGACVLGGVMETHYGAQVCREGVSDTGLYADYRLDNSTLSLEEVEERCREMADLKLPITKLELNLQELGELFQDVLRLQLAEEQMQGPTISIYRCGQSVGVCSGPLLPHTGFLRSFKMLQVSPVTLSGSTGLSGLLRMMGVSFPGEKEREQWVQEQEEARKRDHRRIGKDQELFFFNDISPGSCFFLPKGAHIYNTLTDFIKSEYSRRGFSEVVTPTLYSTSLWERSGHWEHYSENMFTVTCEPHTFALKPMNCPAHCVMYEQRVRSWRELPLRWADFGALHRNEHSGALGGLTRVRRFCQDDAHIFCTPDQLEEEITACLDFVRSVYRVFGFTFHCLLSTRPSSYLGEAALWDSAEQQLEKSLQQFGERWELNPGDGAFYGPKIDIQIRDAIGRQHQCATIQLDFQLPIRFNLQYVGPDGGMYRPVMVHRAVLGSLERMIAILAENFGGKWPLWLSPAQVMVLPVGGDSEHYGREVVHRLKEAGFMADIDDDHGATLNRKVRSAQLAQYNYIFVVGEKERGSGTVSVRTRGGKQLGQRSLEDVMTSLMELRGSRSNRDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXT3</accession>
    <name>A0A3B4DXT3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TRIP11</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017573715.1">
        <property type="nucleotide sequence ID" value="XM_017718226.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000027866"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001428.2">
        <property type="protein sequence ID" value="ENSPNAP00000027866.1"/>
        <property type="gene ID" value="ENSPNAG00000013436.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439689"/>
    <dbReference type="CTD" id="9321"/>
    <dbReference type="GeneTree" id="ENSGT00710000106769"/>
    <dbReference type="OMA" id="RHIAQIS"/>
    <dbReference type="OrthoDB" id="425925at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000237">
        <property type="entry name" value="GRIP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921">
        <property type="entry name" value="MYOSIN HEAVY CHAIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18921:SF2">
        <property type="entry name" value="THYROID RECEPTOR-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50913">
        <property type="entry name" value="GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GRIP" evidence="6">
        <location>
            <begin position="1779"/>
            <end position="1828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1900"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="58"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="187"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="633"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1017"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1098"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1211"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1351"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1530"/>
            <end position="1772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1902"/>
            <end position="1918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1986" mass="223793" checksum="E0744E441FA7392D" modified="2018-12-05" version="1">MSWLGGLSSGLGQSLGQVGGSLSSFTGQISNFTKDILLEGTEEVGDAATELQVSNSKLAELEGSFATQKSEYERLRRINAELEEKLEAAEIQVKQQSLEYRTLLQQKEVEISHLKARQNTLQDEVQKLRSSSQDITSSTSGPAVLPVTTGATTTSSSSYLSRASSSHHGFHGDEVDLSDVLWSQQEINRLSSEVHRLEADVAHWRRVAQASKVPGAENGDQGEILKLQRTIKELREALGREVDEHQHELAALQDAHRQKLSDITKRHREELAEYEERIEELEEQIQSGGAATVTAPSSPQDSSRLEELQATIHSLLEEAGLHKAKHDELMASLEESEHLRAALQREKEEAKAENAELLQNYSRLQSSVSELQTRVQEQEGKAMFKAQQDNEIQALRKALAAAEKEISRLKSSDAEPKADVEHADILELNTIIGTLRQEKEDIEKEKLALVEKVKLAEEREHLIQTEDLQQEVSDLKTQVDQREQSLKQAHLDMDTLTAELEELDRQNQEATQHLITLKDQLAAQKSQAEAEVTRLKSELSDSHNQKEALHQELEAQKQKLSQSAFTLNDLHMGKQQLESTVKELRDKLSKSQELGKEARLEASDLKKLLQEKEAHLSAVHEELSHAGEQGTEVESQRKHLAEKDKEIQDLRKEVAELKVSCEKRSSEDFDLKIENRKLKEESVQALEKVENLDRQLRDSQTSLNRVLLEKDTRIEALKLEKSQLEGELNQLEKRLSEQAKQYQGTIEDLTRARSMDASALQMEHERAVKLNQEKDLMIAELKREIEQMASDHKDTSEMLDITVAGQKQLTELLQEKDTFAASLKAQAADAQRELEASISQAKQEGDALRKSVEDKDKQLGAMKEENSHLKEEIDRLRDQQSRPQLMSEPKTLDIITELETEVAQLKAARDQLQEEAGALRKASEEQRESLLQCQHSLQMQQSELEQARSRHEQSTLNYERLISAKDEEISRLESEVESFSMQRQSQLQTVDILQEDKTRSLSGENGNEKHDLSKVEIERLVKGIKEKETEIIQLNEKNLSLTQQLDQLVVSRDELGKLSQMVLQKDLEIQALHARVSGGHGQEVLLLQQQIQAYAVEREQVLAVLNEKTRENSQLRSEYHRIMDIVAAKEAALLKLQQENQRLSTMSDPSGSQEMFRETIQNLSRIIREKDIEIDALTQKCQTLVTVLQSSSGDSGNGGQGGVSSNQFEELLQERDTLKQQVKKMDEWKQQVITTVKNMQHESAQLQEELLKLQGQVSADSDCSSKLSVDYTRLIQSYEQKERRLGSLSQELAHVQQTISQLSSTKDVLLGKLDSFTQAPEVSTPSIQVAEHFQVSVASPSLRGAAPPIQGESLRQELEALQRALAEKESMIRTLQEINHRLSNSASLSESEQRGHAEELRQARERIDTLQRSLREKDLLIKTKGDQLSQVSENLRNRENDNEVLKQAVTNLKERALILELDVKKLKEENEAVAVKSREKETEFRALQETNMQVSLLLREREFEWKAVSEKAAAMEKLLKDREQGKSGELNQLLNEVRSMQEKAIAFQQERDQVMMALKQKQMETSALQSELQHTKDKEQRQKLELERLRNHLLEIEDSYTREALAAEDRESELRRRVAMLEEKLASSCSAVESASQQASLQVESLQEQLNGVVRQRDEALLQLHAAQEQTNQYAVSLANLQMVLEQFQQEEKAMYSAELEKHKKEKEEWRRKAEALEDRASALQLNLDEANTALESASRLTDQLDLKEEQIEDLKKQVEVRQEMLDDTQQKLTNLLNSTEGKVDKILMKNLFLGYFHTPLNKRSEVLRLMGSVLGLEQDEVEKILDDEGKKGVTGWVSGWLGGSRAVQSVPNTPQRPTHTQNLNGSFSEMFVRFLEVESSPAAPAPKLPVYDIKPLSAPPSGRSTAATNAAGTAGPSKRSGESNPFLAPRSAAIPLLTPGAPGGGGPASGHLLMKPISDALPSFTPMPVSAEASAGAVLKDLLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZZ5</accession>
    <name>A0A3B4DZZ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear FMR1 interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZZ5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029028"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015773.2">
        <property type="protein sequence ID" value="ENSPNAP00000029028.2"/>
        <property type="gene ID" value="ENSPNAG00000014891.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108435816"/>
    <dbReference type="GeneTree" id="ENSGT00940000166439"/>
    <dbReference type="OMA" id="NEQKGNR"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032747">
        <property type="entry name" value="NUFIP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28333:SF2">
        <property type="entry name" value="FMR1-INTERACTING PROTEIN NUFIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28333">
        <property type="entry name" value="NUCLEAR FRAGILE X MENTAL RETARDATION-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15293">
        <property type="entry name" value="NUFIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029028.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="56433" checksum="977156DF6E372BAE" modified="2024-11-27" version="2">MEGNKTSGLQQCEVKHLDEKDPVLSLNGEVTLSSGYITNGYSTKAAADNDGSGSERGYTTPKKRRARRSSMKGTENVNRDKEKAVQQGIMSYSKQDLETSGQEAAGKVVNSRPNCTRTCLKTEVHTGVRRTGVQEMTPVGEVQCKNSDSRASGPISKKTDDKVSKTKLTSSVTSKEDSWTLFKPPPVFPVDNSSAKIVPKISYASKVKENLNKATQASAEVALAQASLKLSQVPMSAVKTSASFTNGPVSGEANGCPSVGTLFNSAASTAPSASSNPGEENVASTLDTSCSSSACPSAPEPRKSSLFVYPHKPLNMQTVLPSARHTDDPTAQTNQKALGDIFQNQWGLSFINEPNAGPEGTGASQLTVSGLEVADITFQGESLQFFNQPGLDPTSGVSLSAPKEVEKRTSGTDAALKACSTSCAASEDPPQVQPESQEEQMLENRSAEVSCKDLDTEPAVSQVNSLVGPSKAQNYIKDHDRANSWGLFDLRTAVLYHTKEFEYLVSLRKQDPKRVVYYEETLDGPDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E2X1</accession>
    <name>A0A3B4E2X1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017551765.1">
        <property type="nucleotide sequence ID" value="XM_017696276.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E2X1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030952"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036452.2">
        <property type="protein sequence ID" value="ENSPNAP00000030952.1"/>
        <property type="gene ID" value="ENSPNAG00000018001.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426637"/>
    <dbReference type="GeneTree" id="ENSGT00940000157079"/>
    <dbReference type="OMA" id="HTHFCSS"/>
    <dbReference type="OrthoDB" id="6078042at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000005">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF36">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEURL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NHR" evidence="8">
        <location>
            <begin position="35"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="261"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34376" checksum="43D26F570A9D1559" modified="2018-12-05" version="1">MQPSPQLVKKNKTKMGIQVQDSHGCCGCGSGCLGPLTFHSEAKGQLVTLSEDGRRATRDTTSFRHGLIFSSRPLRIMEKVHIRIERSVRAWHGAMRVGLTSVAPGSTTIPSLAIPELTNSPGYWAIPVPEHQCLPRSELIFWVSHCGTLHVKTDDGQKHSLKTRVDTHKPMWAMIDVYGQTNAVLLLGSIKKCLFTTRRSCPVPTVEPTDDNCGYSEIPIKTVERMMGQKSLGEPGNQENLSLPCSQNNSQNTSNESMEECVVCYSDIANVMLICGHRCVCSQCALRVFSEFGSCPLCRHHLHPDHCYKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E351</accession>
    <name>A0A3B4E351_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RAB11FIP1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000031037"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036507.2">
        <property type="protein sequence ID" value="ENSPNAP00000031037.1"/>
        <property type="gene ID" value="ENSPNAG00000018143.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165511"/>
    <dbReference type="OMA" id="HTRWDHI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045055">
        <property type="term" value="P:regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08682">
        <property type="entry name" value="C2_Rab11-FIP_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000070">
        <property type="entry name" value="rab11 family-interacting protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037245">
        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037789">
        <property type="entry name" value="FIP_classI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15746:SF22">
        <property type="entry name" value="RAB11 FAMILY-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15746">
        <property type="entry name" value="RAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="FIP-RBD" evidence="8">
        <location>
            <begin position="1293"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="314"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="754"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="804"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1002"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1119"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1211"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="244"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="317"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="364"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="577"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="805"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="827"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1119"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1155"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1241"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1268"/>
            <end position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1379" mass="153183" checksum="DFB10E4A72C33F87" modified="2018-12-05" version="1">MSLAEQSQQWYPTSVQVTVLEARNLRLKGKNGTNDAYAIMQVAKDKFSTSVAEKSVAPAWKEEATFDLPLFHRGNADRCTLCIIIMHRALVGLDKLLGQAIINLVDLQENKARKKTDWYKLVDKNGKADKERGEVLLDIQFMRNNMTASMFDLSMQDKPRSRISKLKDKVRGKKKDGLSDSASAIVPSTVNQVLTDSEGEDEANPNSPKGKKSSKLKSLFAPKSNLHRGVSQSMSTLGTLPEKNSSLSSSRSSGLNGESPDVKKKFKFLGHKRTSSTDSKVSLGPFSLLNRSKQNIPEQNNLCINGSHVYAEEQEPKPSFGSSLSLSSSAKGSVEDLRKYHERNASDVSADSFKGLSIPTYKPEPVEKALHIPQHPQEDEEKKHKKTEGRLQEVLERQEEQEKIRQQEREEKRRRQEEEERKIRLEEEQRKRLEEEQRQRKRREEEAKRVEEQRRQEESRVAERLTSIFGLGKKKEEPSPPDVEDPKLMNVPASTNPFEEIPLSPDSTNPFLGESPAKAQKEERSTFTPTPPASAFPGRTAKVSAVKPRLALSPKPETDNTDPLVSPVTSDIEPTVPGSSLSPKQSPCSDPFESFAMFSEIHSSLAPPKPQRTFAESPRGSMENLSGADYTASMSEKKHRALQPPSYPGGSSKNGNHHPENEVHSPTSQQGQRPPLPLPDYDSLFPKKRHGVMTDTRWEHIIAEVNQRKMDSHLKNIEEEMSVDAPNEPVPNKSEIIKERSLITNQHQKQYLEASSVSTTRMEPKQALIPPKSTTSATARQVLEVNSKQVLTPEPVYATVEKSNHFASRASTPKVQQDPQVKEKRTFSTAVSAVAQPSETKEKPTPASRAVKTSNSLENSDAKALRSDSAPKVQQEAQVKEKRTFNAAVNAVSQPSKMKEKRTLATRTVDKFNSLQNSSSDDDTTELPSAKPRRKVAEKEPVILVQPEKPTPLSLTLPEKAQVERKKDSMTQMSSWENKGLEGRMQAPVNVSNITRIAKNENVPKEQPVADASKGPEKEQIDSDPFPNDKLISLDPWAIPEQDTHQDDLFTSGLKKATKSNDLGLTPEDFDNFFGSTESKNNTHEFFGSVASKDEMDPFFDSVTSKGKKDSFFESVAVKDETHSKESHKNSSPLLQGRYSQRKKLAPQPPVNLEKTVKEKRTLDKPAPQFMPDADDVELTVMSVAGQSNSETTIAASKADLWGMDSFAPPSQALSSSATPELGSGGMSGGKNMLRAWVSPSEIQSVSSQTAGGGALTSRRPHPVKPLSSTESQSPSSISVGKDLKAPIIQEVAEKSKTAESGPYTQLTQEELITLVVKQQTELSKKDAKILELEDYIDNLLVRVIEEKPSILLALNSKIPYVCPYDRHHTITHYQELLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E6Z4</accession>
    <name>A0A3B4E6Z4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD repeat-containing protein 91</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">WDR91</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat WDR91 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E6Z4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000031081"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019741.2">
        <property type="protein sequence ID" value="ENSPNAP00000031081.2"/>
        <property type="gene ID" value="ENSPNAG00000004862.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001566"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141039">
        <property type="term" value="F:phosphatidylinositol 3-kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056327">
        <property type="entry name" value="ARMC9_CTLH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039724">
        <property type="entry name" value="WDR91"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13083">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13083:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23138">
        <property type="entry name" value="CTLH_Armc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="ARMC9 CTLH-like" evidence="8">
        <location>
            <begin position="47"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="554"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="596"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000031081.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="743" mass="83391" checksum="7782348DB5C53984" modified="2024-11-27" version="2">MASAVERTDDVVREYLIYRGFTSTLKHLDVEIKADKEKGFRVDKIIDQLQQFIQGYDLAGLKEYWSYLDRRLFCRLEDVYRPTVNKLRTSLFRYYLVHTVQNQEKTQEFFQKQAVELQGQVEWRDWFVLPFIPAPEQNPAFSPYFSRQWADTFLVSLHNFLSVLFQCISLMFSPQPVLLSFDAEVQRTTSIQEENEQLRQILFALQGESKPKKSEEVVHHKLPAYVQHMDRLGDTELDLVTSQRTANPSTPSRNFFSTFLPQGRRICMCKSNAHFRRITEGWITGSCVDSEKISLNSVFDLQAVKAKDPTASKDSKMTVTVEPGTGHSRQKRHMDHEKERKELFSKHPQQSSEKKAESSDTETQPECETSDVPSNQARVGGEVGGAGAGPEQPFIKLSQEEYGEHHSSIMHCRVDCSGRRVASLDVDGVVKVWSFNPIMQTKATIMSKSPLLSLEWATKPDRLLLLGSGVGTVRLYDTEAKKNLYEMLIDEMCFCRILSLACSPSGTSFVCSAAANMSRPGSITETVPRASLPVSGQLLLWDTKTVKQQLQFALEPGPVAVNCAAFNHNGNLLVTGAADGRIRLFDMQRYESVMSWKAHDGEVYSVEFSYDENTVFSIGEDGKFIQWNIHRSGVKQSEYSLSQDAVGPFVLSGYSGYKQVQVPHGRLFAFDSEGQHVLTCSSTGGLIYRLNKGESGLESVLSLGGHKAPVVTVDWCSAVDCGTCLTASMDGKIKLSTLLAQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E9P2</accession>
    <name>A0A3B4E9P2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FNDC7</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E9P2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022562.2">
        <property type="protein sequence ID" value="ENSPNAP00000032540.2"/>
        <property type="gene ID" value="ENSPNAG00000020532.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004674"/>
    <dbReference type="OMA" id="CGHTYLM"/>
    <dbReference type="OrthoDB" id="9927686at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47135">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47135:SF1">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043601931" description="Fibronectin type-III domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="111"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="201"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="370"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="455"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="541"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032540.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="816" mass="86480" checksum="728A7FBB7A70A543" modified="2024-11-27" version="2">MDLCGIKLWMFLLGMISQNAVQGSSLTISIYTATSKSAVLRWNSYPGSSSYRVTAFLRNSVQPFVFSSFSQNTLMGSINSLSPNTNYTFQVEALDGSMTVLTQSSLDGSTAPDVPTIKTASSKQSQNMTVEFVEVPGATSYIIRAETSDGSFFSEMTVLGTPGTIVGLQPYTDYTLSVMSVNSAGRSQPSEFVQAKTVLASPQLTSSSPSNSSIVVLWAPVPHAVLYSLSIIREGSFLQSPINTTNTSVTINSLEAGTIYCIKVNAWSPESVPGDDVTVCQITRPSTPQSVVLQTVNGMTTLSVSWATVRGADRYTVISSSGLNCTSNSSTCMLTPLTCGQIQYISVTAMNQAGPSLPSDPQQFITFPCPPQPLRVDEIAAGNCSVVWSSVAYVDYYTAFIKRDDGTEQTCNSTGTSCNFNCHCGYSYIMSVFANNQAGSSPSGPVLNQTTLPCCPGNMSVSSVSWESVMISWMAVRGADVYETRAVDAKGELVCNDTSPVCVLSDLTCNTRYSLLVAACNDPRGCNRTCSPQTYQTAPCMPEIVQVTRSNSSSINITWTSTNSAANYTVSVIGSVGNPLTCQSGGTSCTVTGLACGTSYRINAIASTAAGLSMPSYSVPFETAPCCPQNLSVLQVTQAMSNVSWSEARGVQSFIASLTSPRGHAQCHTAQTTCLMGCITCGTNYTVSLQAISSTGSTAECTYHGFSTSECCPSGIRLFRRANNTLRVQWRSSSPLSNYTAVVTGSTSSHSCSPLETGQNTCDVSDITCGDVYSVVVAPVNPDGTVVQFCSSRMYSVFCSGSGVGTVLYRGRRSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EB90</accession>
    <name>A0A3B4EB90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger and BTB domain-containing protein 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Zinc finger protein 151</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ZBTB17</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017564471.1">
        <property type="nucleotide sequence ID" value="XM_017708982.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017564477.1">
        <property type="nucleotide sequence ID" value="XM_017708988.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017564492.1">
        <property type="nucleotide sequence ID" value="XM_017709003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EB90"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000032569"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022575.2">
        <property type="protein sequence ID" value="ENSPNAP00000032569.1"/>
        <property type="gene ID" value="ENSPNAG00000020602.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434065"/>
    <dbReference type="CTD" id="7709"/>
    <dbReference type="GeneTree" id="ENSGT00940000159957"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18206">
        <property type="entry name" value="BTB_POZ_ZBTB17_MIZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001485">
        <property type="entry name" value="Krueppel-related zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000325">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002210">
        <property type="entry name" value="Zinc finger and BTB domain containing 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000831">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000048">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001022">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001032">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000225">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 17 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000346">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 17 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001325">
        <property type="entry name" value="zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF52">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="17">
        <location>
            <begin position="24"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="458"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="542"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="570"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="598"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="626"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="654"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="777"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="119"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="840"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="212"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="283"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="856"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000032569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="883" mass="97060" checksum="039C3B0FB3D3D038" modified="2018-12-05" version="1">MDFPWHSGKVLEQLNHQRQLGLLCDCTFVVDGVDFKAHKAVLAACSAYFHTLFLDQKDVVHLDISNAAGLGQVLEFMYTAKLTLNPQNVEDVLAVATFLQMQEIVNACSAYKSLAGPSASNSGQVEGHQSLVKKDDVASVEGEARDVSAENGVPEADEAPSSSETTESQQATCSTTERQSTGAQQSGSRVGRPLKLGQCARQKKAEVPVTKTDTEESATKEVMEYQDDPSDADYTPKFSQRPSARRSYISTRRSKSKYAAEILSSQNDASSEEGISKPATEENMTEEQEEEEEPGGEEQALEEEDGQMEAEDEGTSGSVDGEEGKCVRSGAMAERSESRAYGSVTHKCEDCGKKFTHTGNFKRHMRIHTGEKPFTCRDCNKAFSDPAACKAHEKTHSPLKPYCCSTCGKSYRQISLLNLHRKRHTGEARYSCDDCGKLFTTSGNLKRHQLVHTGEKPYHCEYCERAFSDPTAKMRHLETHDTDKGHKCSQCDRKFNQLGNLKAHMKIHIADGPLKCKECGKQFTTSGNLKRHLRVHSGEKPFVCMHCQRAFADPGALQRHVRIHTGEKPCLCLICGKGFTQASSLIAHVRQHTGEKPYVCDRCGKRFVQSSQLANHIRHHDNIRPHKCHTCNKAFVNVGDLSKHIIIHTGEKPFLCDKCGRGFNRVDNLRSHVKTVHQGKAGMKRLVVADDDGEDKLLTASASASELDDNEVNIVTVTTDDIVTLATEALAASAVAQLTVVPVAASVSADETEALKAEITKAVEKVQEADPNTQILYACDSCGEKFLDASSLAQHVRIHTAQALVMFQADSDFYQQYAADGTWQATEQVIQGGELLFRTREGEEEAGDGSQGEIAQTEEGDDQEEPQTDDGTETVPICETQTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EHX4</accession>
    <name>A0A3B4EHX4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit f, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase F chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EHX4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030038.2">
        <property type="protein sequence ID" value="ENSPNAP00000036097.2"/>
        <property type="gene ID" value="ENSPNAG00000005345.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167489"/>
    <dbReference type="OrthoDB" id="8921675at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019344">
        <property type="entry name" value="F1F0-ATPsyn_F_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13080">
        <property type="entry name" value="ATP SYNTHASE F CHAIN, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13080:SF13">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10206">
        <property type="entry name" value="WRW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036097.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="55481" checksum="9CFE62818FE8C5C6" modified="2024-11-27" version="2">MSLEPCPYCGKPFKRLKSHLPHCKMSPGSKTTKNNKAPAESLTTSPKDTPPKEKKSKVNVFDKKLNDSSFQTISKISAKPKVSMTAEKVNMDELTVTKALTSERRNPATVKPKIKWLAKREQEMAKQAILLTPKGSKLISDSLQEKDNCLVETSQCQVKGALQTEGQNQKMLTGAKLTTLVTTKKFTAPDVLHVTWTTGSQAERSMPKCKAKGNILEEIQITPKALTKDHASTPTVKERADFSFFQTKTCVWDHFKHGLYARRSGIMSLLHPTAETHEASISNCKNTTVFLHKAHIENNQNSVTKASSAPLLASAQTSVKRPAEDLLTLVLESKTVVKRTPEMAAGYDGALFSPFRNPEISSNEDLWAKCESTRSRPQSQGPVTEQRLGDVRLGELAAWLGARTPKSPREAMTMLSRGWQWYYRKYIDVQKGGIGGITMLIAGYCVLSYIWSYPHLNRNNSSVYTSDLSRIPLFILWITQSIHPSIHFLSRFSVRVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EIA3</accession>
    <name>A0A3B4EIA3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">GYG2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Glycogenin participates in the glycogen biosynthetic process along with glycogen synthase and glycogen branching enzyme. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Self-glucosylating initiator of glycogen synthesis. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23360"/>
            <dbReference type="Rhea" id="RHEA-COMP:14604"/>
            <dbReference type="Rhea" id="RHEA-COMP:14605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140573"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56560"/>
            <dbReference type="Rhea" id="RHEA-COMP:14606"/>
            <dbReference type="Rhea" id="RHEA-COMP:14607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140574"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:56561"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017570577.1">
        <property type="nucleotide sequence ID" value="XM_017715088.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017570578.1">
        <property type="nucleotide sequence ID" value="XM_017715089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EIA3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035029"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027497.2">
        <property type="protein sequence ID" value="ENSPNAP00000035029.1"/>
        <property type="gene ID" value="ENSPNAG00000024690.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437758"/>
    <dbReference type="CTD" id="8908"/>
    <dbReference type="GeneTree" id="ENSGT00940000161628"/>
    <dbReference type="OMA" id="PAFHKYG"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000092">
        <property type="entry name" value="Glycogenin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="261"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="319"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="397" mass="45019" checksum="905733B1C76E3A82" modified="2018-12-05" version="1">MTEAHQAFVTLATTDVYCKGSLVVGKRLRKHGTTRKLVVMISPNISRETHVSLEDVFDEIIVVDVLDSRDSAHLSWLRRPDLGVTFTKIHCWTLTHYTKCVFLDADTLVLGNVDELFDREELSAAADPGWPDCFNSGVFVFRPCLDTYLRLLEHADLHGSFDGGDQGLLNSFFSDWATKDINKHLPFIYNLSASALYTYLPAFHKYGHQARIIHFLGATKPWNFRYNQHPSSGVDPLDFSRNLEHYVNLWWEEYNSQTDQQRKQPLAALQTAPQPQSQESQSSEEETLSQPHGDLTLNVPSSCCQLEKMMGNERILASSDLSDAEESDCSEFQGSSSDQEEEDVGTESAEKLTVSEPVAAAVETEVERRRQWEEGRADYLGKDAFENIQKRLDQFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJ25</accession>
    <name>A0A3B4EJ25_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">AARS</name>
        <name evidence="16" type="synonym">AARS2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. Interacts with ANKRD16; the interaction is direct.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJ25"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030965.2">
        <property type="protein sequence ID" value="ENSPNAP00000036437.2"/>
        <property type="gene ID" value="ENSPNAG00000027249.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158246"/>
    <dbReference type="OMA" id="NCLEIWN"/>
    <dbReference type="OrthoDB" id="2423964at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF8">
        <property type="entry name" value="ALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="15">
        <location>
            <begin position="33"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="633"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="637"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="748"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="752"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036437.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="993" mass="109559" checksum="8D70EF578B5998BB" modified="2024-11-27" version="2">MAACVRALRGCARLPVSASSRRCSALSRARGQYTSQKVRRTFIEFFTEQHRHRAVPSAPVRPRGDPSLLFVNAGMNQFKPILLGCADPRSEMASYQRVVNSQKCVRAGGKHNDLDDVGRDVYHHTFFEMLGNWSFGDYFKVEACAMAWHLLTEVYEIPAERLYVSYFSGDAASGLPADEETRHIWLSLGLRPDHVLPFGMKDNFWEMGETGPCGPCTEIHYDHVGTRNAAALVNADSPDVVEIWNLVFMQYNREGDGGLRALPQCSVDTGMGLERLLTVLQGKRSNYDTDLFTPLLSAIHQCSRAPAYSGRTEQTDAGRVDMAYRVVADHVRTLSVCIADGVYPGMTGAELVLRRILRRAVRFSTEVLHAPEGALASLVPTVAHILGDAYPELHAETGRIMEIINVNEAQFLSSLKQGRRVIDRTLSKMDRDAVNFPAAVAWSLHRNLGFPLDLIDLMLEERNMAVDKHGVEQLAAEQEQLQSQSEGGAEERGLHMDLQSLAELQSRGVPHTNDSPKYSYSLQHNGTYVFSPCRASVLALYCNQSLVSEVSEGQRCGMVLDQTCFYAEQGGQMHDQGYFTKDGLQDVLFPVECVRLAGGYVVHEITAAETLRTGDQIQLHVDETQRLACMVKHTATHVLNFALRELLGSGVVQRGSHVTADRLRFDFSYKASLNVSQLQEVERMVQDIIHQNDVVHMQEVPLARANQIAGLRTVDEVYPDPVRVVSVGVAASELLSNQKHKHTSVELCCGTHLLQTGGIGDFVIVSERQMVKGISRIIAVTGEDAKQAREAGQVLLQEVEALTARIATDHTPSLATAQRLSKEVGLLTDAVESTPIPQWQRRDLQTRLKALQRSSNTTIRKLEMKEAAAKAKELLNRHSNKAVLVDTLETDSISVVMKTVNQLSERAPDSLIMLLSYLQPSGRVLCACQVPKGQAAVSASEWALAVCSRLGGNAGGSATVAKGVGTATDVTDLLEMMHWAEEFAHNKRQQTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJV7</accession>
    <name>A0A3B4EJV7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017544765.1">
        <property type="nucleotide sequence ID" value="XM_017689276.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJV7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036737"/>
    <dbReference type="Ensembl" id="ENSPNAT00000031734.2">
        <property type="protein sequence ID" value="ENSPNAP00000036737.1"/>
        <property type="gene ID" value="ENSPNAG00000027829.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108416249"/>
    <dbReference type="CTD" id="560091"/>
    <dbReference type="GeneTree" id="ENSGT01030000234624"/>
    <dbReference type="OMA" id="CIPVEDK"/>
    <dbReference type="OrthoDB" id="6079678at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000027">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000031">
        <property type="entry name" value="Cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000123">
        <property type="entry name" value="Protocadherin beta 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014868">
        <property type="entry name" value="Cadherin_pro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009122">
        <property type="entry name" value="Desmosomal_cadherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF78">
        <property type="entry name" value="CADHERIN-LIKE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08758">
        <property type="entry name" value="Cadherin_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01818">
        <property type="entry name" value="DESMOCADHERN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01820">
        <property type="entry name" value="DESMOCOLLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01055">
        <property type="entry name" value="Cadherin_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 14 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017203383" description="Cadherin domain-containing protein" evidence="18">
        <location>
            <begin position="23"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="669"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="126"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="234"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="342"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="455"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="890" mass="98030" checksum="3B6C0C4A82F8A571" modified="2018-12-05" version="1">MSRCSLFYISLLVLVLRYSVESCVQRHVQAQVPEKLQAGYVVSKVNLDHCGFRQLYLVSNDPDFAVQTDGTILTADVITVPDDGKSFWVLVQDQRGHKWKVDVNLSPIDQVSQKSSSAVLRRAKRRWSPLPFNIRENDKGPFPKDVERIASDSSVNYTIKYSIEGPGVTTTPINLFSVDSINGMLRVNFAVDREEYPQFIFTARAFDRYTNQEKDLPLPITVNVQDENDNAPQFSSPLIFSVEEQSNPGTVVGQVNATDRDEPNTPHTSIRYSLLNGTDLFAINYDSGLITTRTGTLDRETQDRYFVRVEIKDMGGAPNGLSNQGLASIFLTDINDNPPTFKQSLYTAQVKENQANVLVLRIPVDDKDLKGTPNWKAVYEITKGNESGNFLIRTDPATNEGLLYVAKPLDREKQELMKLELMARNEAPLVRSSSSWKKVPIELTVTNEDEGPEFSAPILRLKVKENVPNGTLIGTYTAIDPETKSSKGIKYYKVTDPGSWINVMETTGDLKVANTIDRESPLVYNNTYNITVKAVDESQKTGTGTVLILIEDVNDNVPIIPKPDLVLCSRDGQRSSILVNAVDYDESPYSGPFTFELSKESEGKWKIKDPKPGTSVVLEQVVDMPNGVYKVPLLVKDLQGVGEEQIVSVHVCECVKEGECAAQRISSRLGVWAVLAMLLALLLLLLLCLLFVLVCSTKGEKLRMTDEYDGTGGMLLKSNTEAPGEEVKDALLVTPGSGIDGGGAMEHQTVVGAAGGALGQQAIHAGSMYQGGTQGYMTNQSMYSTGQYGGGFYGNSSYNKFSDMSILDTWRTNELYLDNKLVYFGEEEDGRFAADLLKSYGYEGVGSPAGSVGCCSVLSNQDSLDFLDSLGPKFRTLADVCMSKTERGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EK63</accession>
    <name>A0A3B4EK63_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">L-type lectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017569594.1">
        <property type="nucleotide sequence ID" value="XM_017714105.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EK63"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000036280"/>
    <dbReference type="Ensembl" id="ENSPNAT00000030602.2">
        <property type="protein sequence ID" value="ENSPNAP00000036280.1"/>
        <property type="gene ID" value="ENSPNAG00000027001.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437182"/>
    <dbReference type="CTD" id="403038"/>
    <dbReference type="GeneTree" id="ENSGT00940000155596"/>
    <dbReference type="OMA" id="GCSIDYR"/>
    <dbReference type="OrthoDB" id="270293at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000017">
        <property type="entry name" value="Vesicular integral-membrane protein VIP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF20">
        <property type="entry name" value="VIP36-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017183685" description="L-type lectin-like domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="303"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="14">
        <location>
            <begin position="29"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000036280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="339" mass="39256" checksum="5488A321AD18061E" modified="2018-12-05" version="1">MARMMVATYLMFIAYLHSSLSDDGHEMEEFLKREYSLTKPYQAIGVSGSSHWELMGDAMVTTDQVRLTPDMQSKQGAVWSRMPCHLKDWELQVHFKIHGQGKKNLNGDGLAVWYTKERMQKGPVFGNKDFFTGLGVFVDTYPNEEKHIEAQKKRYTPRTQRIFPYVLAMVGNGSITYDHERDGRPTEIGGCNAMVRNLKHDTFLFIRYVRRRLTVMIDIDGQHEWRDCLDIPGVRLPMGYYFGASAITGDLSDNHDLISLKLYQLTVLRSKQEEEDEEEVTIPSVDNMELHRNYYEGEGMSGIAIFFTVLFSMLGLFLLIVVGLVVYSHWNESRRKRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ER22</accession>
    <name>A0A3B4ER22_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017548365.1">
        <property type="nucleotide sequence ID" value="XM_017692876.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ER22"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041198.2">
        <property type="protein sequence ID" value="ENSPNAP00000037744.1"/>
        <property type="gene ID" value="ENSPNAG00000029764.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424690"/>
    <dbReference type="GeneTree" id="ENSGT00950000182933"/>
    <dbReference type="OMA" id="CLLPFDR"/>
    <dbReference type="OrthoDB" id="9948609at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047123">
        <property type="entry name" value="MYADM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068:SF3">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER MYADM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="10"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="283" mass="30984" checksum="EF85EB7D8E10BB5C" modified="2018-12-05" version="1">MPVTFGELSTLTTPLSVTRFWALFFGCLTFSLAAVVDTSSKEYFNTLHTFCMATWCFFFILTFFIVAVNFIQFHSLLPLSWKNLTATVAALGMLMTMTATLAFPWILVGQSKAKPREVAASVGSFLTSLAYIVELQLIRTQGSQQQRGYMASVPGLLKVLQVFGSCVALVLIVPQKTEEDWRISISATVYSLSLAASLGTVLVMVGDCAGRCPVPFDRLLAGFSTLGVLLYMMATVICITRVLSPIHDIESFVLMSETVIASITLLAYTVDLAFSVKLLCDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ERC3</accession>
    <name>A0A3B4ERC3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyl-CoA-binding domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Acyl-CoA binding protein which acts as the peroxisome receptor for pexophagy but is dispensable for aggrephagy and nonselective autophagy. Binds medium- and long-chain acyl-CoA esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG37 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037393113.1">
        <property type="nucleotide sequence ID" value="XM_037537216.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393114.1">
        <property type="nucleotide sequence ID" value="XM_037537217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ERC3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037844"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034724.2">
        <property type="protein sequence ID" value="ENSPNAP00000037844.2"/>
        <property type="gene ID" value="ENSPNAG00000029918.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431881"/>
    <dbReference type="CTD" id="553674"/>
    <dbReference type="GeneTree" id="ENSGT00940000156350"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00435">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016347">
        <property type="entry name" value="ACBD5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF6">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002412">
        <property type="entry name" value="MA_DBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="ACB" evidence="16">
        <location>
            <begin position="9"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="165"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="288"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>an acyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>an acyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>an acyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>an acyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR002412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR002412-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037844.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="511" mass="56963" checksum="D265EE4F42C6CCE9" modified="2024-11-27" version="2">MELDTKPIHEQRFDAAVKVIQSLPTNGSFQPSNQMMLKFYSYYKQATQGPCNIPRPGFWDPVGKAKWDAWHSLGDMPEEEAMTAYVDDLKLILESMPMTDEVEQLLQVLGPFYELVDEKKKISEVSDLTTARLERFAKQLEGFGNMITAPSKSVTKSIIRTMEMNGTLDSYPTKKGDAPKNNIQEIKEQEDRGDEDEKEEDDDDSDDDDEEEEEEEEEEPEQVKKVPAPKKKATNGKAKGVPNGTIGHKVEIMTNGTHSSKSALNGEHLAEEEDAVTQETVIQHVNGFTKDDDEDVSSLHHVASDSDGEVYCDSVDQFGVEEESQGRLSSLREPQPTGWDPKDLPGVQESVQHGGEDWKTSGGGPQRQRLSMDRPGSSVVRRGRASRSPSSGFGTRGPLQGGGGDGERWGTDGAVSGDLNEQIVTALARLQEDMQSVLERLHTLEALTASQARSAVLPSEYLLPPANRAQKKPCWWPFDVSPGTVAFAIVWPFVVQWLIRLYFQRRRRKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ILF5</accession>
    <name>A0AAR2ILF5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ILF5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086350.1">
        <property type="protein sequence ID" value="ENSPNAP00000038839.1"/>
        <property type="gene ID" value="ENSPNAG00000014535.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160082"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="94"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="436" mass="49672" checksum="4FE2683F350CDBFB" modified="2024-11-27" version="1">MQEGSKCVCLYQERRYLSGWRGFLSSMGISLPPPLCRLGASQRMIQDKGPRIPEHLLSSMPGPEKLRMEWSLPGFITMFLPEFPHRTIPGHEHFQVLGYIAKGSFGPILKVKDKSKEKTYAVKVIPKSEILRLGVLDQSKEEVIVQRQVRHPFVHDLQDCWQTQRHLYIMCDYCSTGDLYTYWVMIGQFGEDAVKVFAAELGSALGFLHDFGIIHRDVKMENILLTDQGHLRLADFGLSRRLERGGRAFTICGTIQYMAPEVLSGGPYSHAADWWSLGILLFALATGKFPVPPEPDHCSMLRKVRCFPYEMPRTFSPPFALLLTELLCKTPTRRLRTLERFKRQTFFRGTPFDSQLLQKTPMELILELKTRPDRPAKARRGLTLEPFPNFDCDFLVDSTSTPAQEMSPTLANIELVEALIQGHVSQGNVPQQEVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IPV1</accession>
    <name>A0AAR2IPV1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">POGLUT3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[EGF-like domain protein] + UDP-alpha-D-glucose = 3-O-(beta-D-glucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58116"/>
            <dbReference type="Rhea" id="RHEA-COMP:14610"/>
            <dbReference type="Rhea" id="RHEA-COMP:16010"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140576"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-(beta-D-xylosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62016"/>
            <dbReference type="Rhea" id="RHEA-COMP:16010"/>
            <dbReference type="Rhea" id="RHEA-COMP:16011"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KDELC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037401599.1">
        <property type="nucleotide sequence ID" value="XM_037545702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IPV1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060924.1">
        <property type="protein sequence ID" value="ENSPNAP00000040052.1"/>
        <property type="gene ID" value="ENSPNAG00000008547.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413990"/>
    <dbReference type="GeneTree" id="ENSGT00940000159028"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046527">
        <property type="term" value="F:glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000419">
        <property type="entry name" value="KDEL (Lys-Asp-Glu-Leu) containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF18">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043961227" description="Glycosyl transferase CAP10 domain-containing protein" evidence="11">
        <location>
            <begin position="20"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="10">
        <location>
            <begin position="26"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="12">
        <location>
            <begin position="231"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="514" mass="58648" checksum="430D0FFDF1A22529" modified="2024-11-27" version="1">MAVGLIALAALILFNNVDSGLFQTHKDDVSPEQCLVWGPGLEPRSVLPVRHFYIQAVSESGQVISTSPGKDSFRVRITSLLQKEHVRIHVPPPLDRGDGSFLVRFRMYGSSVHGLKVEVFYKDKPVAKSPYTLKGVVYHEYCDCPEADASAWQSVMECPSEEPQIQQDFSSFPSINLRQLLEEVPKRFSSRGGLIHYTVINNQVYRRCLGRYTDFKMFSDEMLLSLTRKVKLPDVEFYINVGDWPLETRKGDAVPGPVPIISWCGSTETRDIILPTYDITHSTLETMRGVTNDLLSVQGNTGPAWVNKTGRAFFRGRDSREERLNLVSMSKKNPDLLDAGITAFFFFREREKDLGKAPLVGFFDFFKHKYQVNVDGTVAAYRFPYLMLGNSLVLKQDSPYYEHFYTHLRPGQHYVPVKRTLSDLIQKIKWAKENDAEAEAIAKAGQAVARDVLQPHRLYCYYYTVLETYAARQIGQPSVHPDMELVPQPSDHSAVCDCERRMEPQEFRQKREEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IQY4</accession>
    <name>A0AAR2IQY4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AMOP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isthmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017542157.2">
        <property type="nucleotide sequence ID" value="XM_017686668.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IQY4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000061404.1">
        <property type="protein sequence ID" value="ENSPNAP00000040444.1"/>
        <property type="gene ID" value="ENSPNAG00000033513.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413947"/>
    <dbReference type="CTD" id="100535376"/>
    <dbReference type="GeneTree" id="ENSGT00940000163359"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000033">
        <property type="entry name" value="Isthmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051867">
        <property type="entry name" value="Angio_Inhib/Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239">
        <property type="entry name" value="ISTHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239:SF28">
        <property type="entry name" value="ISTHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043636029" description="AMOP domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="8">
        <location>
            <begin position="282"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000040444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="457" mass="52259" checksum="3CF346477A54F4AA" modified="2024-11-27" version="1">MRTRGTLLHWTAFLTLVLLSVQAFPALTPKAGEAKPGSSAALRTGGQAGSVDVKRTLAELQQQNQLKTRSPSRRHRRRWSQLRSEGVLPKPAPEEEEPFILDLKNFPDLANADLGSQNPNIQVTIEVVDDPQMEVEMDLAKEGRNDWSLSSEQWLDHKKLFWPLFWEYHDSSEESPGQASLEENGEDLNYDGEDSLLSGVGVDWGRRWKGWDSFENYEYEEEEWSDWSPCSVSCGNGNQKRIRSCGYACTATESRTCEQQRCPDDLNAVTELIPHEMNDTDAFDTDMDSCEKWLNCKNDFLQKYLHQVLTELPSCPCVYPSEVVYSAVNIFDEKLRKTYRWRDASGPKERLDIYKPSAKFCVRSMLSFDSTTLAAQHCCYDDHMKLITRGKGAGAPNLISTEFSPELHYKVDVLPWILCKGDWSRFHSVRPPNNGLQCAENPQEDVYMNELEEAREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITL0</accession>
    <name>A0AAR2ITL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2ITL0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000058998.1">
        <property type="protein sequence ID" value="ENSPNAP00000041379.1"/>
        <property type="gene ID" value="ENSPNAG00000030783.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234537"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057667">
        <property type="entry name" value="HTH_SB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25787">
        <property type="entry name" value="HTH_SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sleeping Beauty transposase HTH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="2">
        <location>
            <begin position="71"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31686" checksum="B495BDCDB9EAA18A" modified="2024-11-27" version="1">MVKTKELSKDTRNKIVDPHQAGKTESAIGKQLGVKKSTVGAIIRKWKTFKTTANLPRSGAPRKISARGVKMITRTVSRNPRTTRGDLVNDLPRAGTNVTKATVSNTLRRQELRSYSARRVPLLKPVHIWAHLKFAREHLDVPEEYWDNVLWSDETKVELFGTNTTRRVWRRVNAELHPKNTIPTVKHGGGNIMLWGGFSWLRKKHFKVLEWPSQSPDLNPIENLWRELKVRVACRQPQNITALEEICMEEWANITATVCANLVKTYTKRLTSVIANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IUC4</accession>
    <name>A0AAR2IUC4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IUC4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045664.1">
        <property type="protein sequence ID" value="ENSPNAP00000043275.1"/>
        <property type="gene ID" value="ENSPNAG00000024016.2"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
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        <property type="entry name" value="LDL_receptor-like_sf"/>
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        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
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        <property type="entry name" value="NOTCH1_EGF-like"/>
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        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
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        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
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        <property type="entry name" value="LDLRECEPTOR"/>
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        <property type="entry name" value="ASX_HYDROXYL"/>
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    </dbReference>
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        <property type="entry name" value="LDLRA_1"/>
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        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="20" id="KW-0168">Coated pit</keyword>
    <keyword evidence="17 23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 23" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6" id="KW-0964">Secreted</keyword>
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        <location>
            <begin position="810"/>
            <end position="832"/>
        </location>
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        </location>
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        <location>
            <begin position="750"/>
            <end position="786"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="883" mass="97208" checksum="AEC90A6DAA06D6F9" modified="2024-11-27" version="1">QDKPAATFPHYAAACDSRNFQCGNGKCITLRWMCDGTDDCGDGTDELPAACLSKTCRPTEFSCGGRLNQCVPNSWKCDGNADCENGADEAGCGSRQCLSGEFRCANGQCVSRAFVCDDESDCDDGSDEAQCLPSTCGSSSFRCNNSACLPRLWACDGDMDCPDGSDEWPQNCGVRPSVPPSRTRPCSILEFHCGSGECIHSSWRCDREADCLDRSDEKNCSLSTCRPDEFQCGDGSCIHGSRQCNQVYDCKDMTDELGCVNASHCEAPSKFKCRSGECISIDKVCNKQRDCRDWSDEPLRECDSNECLYNNGGCSHICNDLKIGYECLCPSGFRLVDKKRCEDIDECANPDTCSQICVNQLGSYKCECEEGYQLDPATKACKAIGTIAYLFFTNRHEVRKMTLDRSEYTRVISRLKNVVALDVNIATKEIFWCDLSLKKIYRAPMDSASDSSQHSVVIGSGVEAPEGIATDWVHGNIYWTDSIRGTISVATANGKKHKTLFAKDLAKPRAIVVDPIKNFVYWTDWGTPAKIERGGLNGVDRSALVTDNIIWPNGITLDLLNQRLYWVDSKLHTLSSIDVQGGSRRTLIIDHGRLAHPLSVTVFEEKVFWTDVSNNAILSANRVTGEDITKVAEHLSSPEDIVLFHNLKQPRAVNWCMLGNGGCEFLCLAAPQVSRFSPKYTCACPDNMMLDKDMRKCVPGKINNVMLKSVSGFVQDTLSADNHLSIHHVNSSLCAVFLFRTAVPSTLPQVQPKTSATAQPQNPTTTVPPTTKASALSTTTPMPVLHTQPKEGVTETLSTNTPSEVSTQTIALYIVLSLGMLCLLGIGGVLLWRTWKLKNTNSIHFDNPVYQKTTEDKVHICRTFNSDGYSYPPRQIVSLEDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IUY4</accession>
    <name>A0AAR2IUY4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IUY4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059459.1">
        <property type="protein sequence ID" value="ENSPNAP00000043548.1"/>
        <property type="gene ID" value="ENSPNAG00000035002.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000044642"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000063">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF9">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="186"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="403" mass="45737" checksum="E4F678B010677B39" modified="2024-11-27" version="1">MRLMRYKPEAVYVPGKTLVVADALSRSPQIHTEEKTDTHSVVEHYIATVMQSIPASQSRMDSIKAATTTDCELQTVIRYIRNGWPEYRDKVPLDVRAYVKVKNELSETNGLVIRGCRIVIPKSLRAEILKKIHDGHQGLTKCRDRANSSVWWPGLSAELKETVMQCHTCQELRRTQQKEPLISTPLPGRPWKRIALDLCEYNKNDYLIVSDYYSRFLEILHLPSTTSAQVIQRLKTTFARFGIPDEVVSDNGPQFSSAEFKELAQQMDFKHLTSSPHHPQGNGHAERAVQTAKRILKQEDPLLALMCHRSTPCTTTGVSPAELLMGRKIRTTLPILEKNLQPKWPNRKAVMQKDAVEKAKQAFYYNRRHGARLLPPLRPGGAVVTKLDQEKTWVTPAVVSAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IY53</accession>
    <name>A0AAR2IY53_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Multiple C2 and transmembrane domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MCTP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2IY53"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049688.1">
        <property type="protein sequence ID" value="ENSPNAP00000042974.1"/>
        <property type="gene ID" value="ENSPNAG00000001256.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156031"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04042">
        <property type="entry name" value="C2A_MCTP_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08376">
        <property type="entry name" value="C2B_MCTP_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08377">
        <property type="entry name" value="C2C_MCTP_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000050">
        <property type="entry name" value="Multiple C2 and transmembrane domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000068">
        <property type="entry name" value="multiple C2 and transmembrane domain-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000019">
        <property type="entry name" value="Multiple C2 and transmembrane domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013583">
        <property type="entry name" value="MCTP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45911:SF3">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08372">
        <property type="entry name" value="PRT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="540"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="645"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="33"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="182"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="332"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000042974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="724" mass="83044" checksum="4E95D6632BFE0969" modified="2024-11-27" version="1">IHAVSSNIDNTAWKAGETNICSGCNLIIMVIVSVADDGWATSVPGMYKLDILLKRGHNLAIRDRGGTSDPYVKFKLGKKEVFRSKVIYKNLNPVWDERFCLPIDNLHEPLYIKVFDYDFGCHDDFMGSAYLYLDSLELRRFLDVRLDLLDSQCPMQDLGFLELAIMLSPKDGWDREVVRQQQQSQRLSEVHKKSQLWRSMVSISLIEGRDLIPMDQNGLSDPYVKFKLGNEKYKSKTMSKTLNPQWREHFDLHVFDEDGGVLEISVWDKDTGRRDDFMGRCALDLSALSREQTHKLELDLEDGQGQLMLLVTLTASAAVSISDLSIHLLDDPHECQQILNKYSLLRSFCNLSDIGIVQVKILRAEGLMAADVTGKSDPFCVIELCNDRLQTHTVYKNLNPEWNKVFTLNVKDIHSVLEVSVYDEDRDKNADFLGKVAIPLLNIHNGEQKAYILKNKEMTGPTKGVIFLEADVIYNSVKASLRTFETPEQKYIGEESKVSKQLLLQNFNRVKRCIMFLINMGSYINSCFEWESPRRSICAFLAFVLVVWNLELYMVPLSLLLFLGWNYFLLASGKETREGSVVTMEDLLESEDEECEKDEKDSDKKGFMDKLYAIQDVCISVQTALDEVASYGERIKNIFNWTVPFLSWLAIAALCVASLLVYLIPLRYIVLVWGINKFTKKLRNPYLIDNNELLDFLSRVPSDVQVVQYKELKTEHRQSPNKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J080</accession>
    <name>A0AAR2J080_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J080"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081300.1">
        <property type="protein sequence ID" value="ENSPNAP00000043241.1"/>
        <property type="gene ID" value="ENSPNAG00000002222.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153654"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23801">
        <property type="entry name" value="UBCc_UBE2L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000011">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="11"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="163" mass="18826" checksum="88A1588CD9E2E169" modified="2024-11-27" version="1">ADLDNPLRDALYITGKSYELDEIRKSGMKNFRNIQVDESNILTWQGLIVPDNPPYDKGAFRIEIIFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFFKNAEEFTKKHGEKRPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0L9</accession>
    <name>A0AAR2J0L9_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0L9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066545.1">
        <property type="protein sequence ID" value="ENSPNAP00000043376.1"/>
        <property type="gene ID" value="ENSPNAG00000031840.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000202153"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="71" mass="7795" checksum="F2CD8360EFCA137F" modified="2024-11-27" version="1">MLNLLFASFKFDIFENMDPSCVVSMVQAGGGGVMVEMFSWHPLGLLIPINHRLNAAAYLTIVADHLLSQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J1W7</accession>
    <name>A0AAR2J1W7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Jupiter microtubule associated homolog 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">JPT2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JUPITER family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541885.1">
        <property type="nucleotide sequence ID" value="XM_017686396.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J1W7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000077871.1">
        <property type="protein sequence ID" value="ENSPNAP00000044254.1"/>
        <property type="gene ID" value="ENSPNAG00000030972.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000007652"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033335">
        <property type="entry name" value="JUPITER"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="JUPITER MICROTUBULE ASSOCIATED HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17054">
        <property type="entry name" value="JUPITER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000044254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="21435" checksum="54A3D113E643A113" modified="2024-11-27" version="1">MTSTNMFQGLEDGSKPSSRVLRPPGGGSSNIFGGCDDTSASRRPNKMASTVFAPPEEPQGGPKRSNPPGGKSSGIFGEPQAPSTQAKPIPPGGVSNIFGGPQSNPPVKSHPNKPKDNIGLGAAVAPDPPAPQQKVIEEVKAVPVTPEATPRKEEPAAAPAPTATATPEAESSPIQPSEKDHEPRLGPRPRSHNRVLNPPGGKSSVVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J6S3</accession>
    <name>A0AAR2J6S3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="6">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037403266.1">
        <property type="nucleotide sequence ID" value="XM_037547369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J6S3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066448.1">
        <property type="protein sequence ID" value="ENSPNAP00000047635.1"/>
        <property type="gene ID" value="ENSPNAG00000008519.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433263"/>
    <dbReference type="CTD" id="83776"/>
    <dbReference type="GeneTree" id="ENSGT00940000166058"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000004">
        <property type="entry name" value="Cryptochrome-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF9">
        <property type="entry name" value="CRYPTOCHROME CIRCADIAN CLOCK 5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="9">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="485"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="248"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="288"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="386"/>
            <end position="388"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="319"/>
        </location>
    </feature>
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        <location>
            <position position="373"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
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            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="521" mass="60058" checksum="8DACCAF1772E7DE0" modified="2024-11-27" version="1">MKHKCIHWFRKGLRLHDNPALKAALHDCEHIYPIFILDPWFPKNAQVGINRWRFLVEALKDLDSSLKKLKSRLFVVRGVPRDVFPKLFKEWKVTRLTFEVDTEPYSQIRDKEVMDLAKQHGVEVITKISHTLYNIERIIEENNGKPPLTYVRFQTVVKAIGTPKRPVPAPTREDMKGILTPSSDKHDENYGIPTLKELGQDSSTLGPDLYPGGEQESLRRLEEYMQRTGWVCSFEKPQTSPNALSPSTTVLSPYVKFGCLSARTFWWGLAEVYQGRKHSAPPVSLHGQLLWREFFYTAAVGIPNFSKMEGNPNCVQVDWDSNPEHLAAWKEARTGFPFIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGQKVFEELLLDADWSLNAGNWQWLSASAFFHQYFRVYSPIAFGKKTDKNGDYIKKFLPVLKKFPPEYIYEPWKAPKSVQQQAGCIVGKDYPRPIVDHDVVSKRNIQRMKAAYAKRHGDPSSSQTESKGQKRKSASIKEMFTKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JGN4</accession>
    <name>A0AAR2JGN4_PYGNA</name>
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        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="primary">EFHD2</name>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSPNAT00000080972.1">
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    </dbReference>
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        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EFHD1/2"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="84"/>
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        </location>
    </feature>
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        <location>
            <begin position="27"/>
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        </location>
    </feature>
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        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSPNAP00000051208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="232" mass="26530" checksum="F5B35F3DCFF1FA4A" modified="2024-11-27" version="1">MATDELSMKLNRRLKIEEGGQDPVALDEAHENGAHDKSSTASADSELEAKLMRRGELNEGVGEHQQPSMKVFNPYTEFKEFSRKQIKDMEKMFKQYDSEKDNFIDLMELKLMMEKLGAPQTHLGLKNMIKEVDEDLDGKLSFREFLLIFRKAAAGELAEDSGLHVLARLSEIDVSTEGVKGAKTFFEAKVQAINESNRFEAEIRHEQEEKKKQAEEKKQRQAAFKELQSAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JJA5</accession>
    <name>A0AAR2JJA5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoinositide phospholipase C</fullName>
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        </recommendedName>
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    <gene>
        <name evidence="10" type="primary">PLCL2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JJA5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067116.1">
        <property type="protein sequence ID" value="ENSPNAP00000052118.1"/>
        <property type="gene ID" value="ENSPNAG00000029597.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439292"/>
    <dbReference type="GeneTree" id="ENSGT00940000155660"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032228">
        <property type="term" value="P:regulation of synaptic transmission, GABAergic"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_PLC_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08597">
        <property type="entry name" value="PI-PLCc_PRIP_metazoa"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF84">
        <property type="entry name" value="INACTIVE PHOSPHOLIPASE C-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="110"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="9">
        <location>
            <begin position="587"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="703"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1066"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1069"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1097"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1106" mass="124119" checksum="92D7424CD245554A" modified="2024-11-27" version="1">MAEFTENCGLLSPTAGEKAGEEDGKTQREVSVSNGDCGGLSADVVGSAGADRQGLEAATTSVVLDPKSGIPRRTSIIKDGSRQRKERKKTVSFSSMPTEKKISSASDCINAMVDGSELRKVRSNSRVYHRYFLLDADMQSLRWEPSKKESDKAKIDVKSIKEVRTGKNTDTFRTNGIYDQISEDCAFSVIYGETYESLDLVANTADVANIWVTGLRYLISYGKLTLNMIESTQNSLRSSWLGDLFDEDDVSGNKSLSLCAAVQSIKKLNPGLKNVKIELKLKELHKAKDKMGCDVTKEEFIEVYHDLCTRPEIYFLLVQFSSNKEFLDSKDLMIFLEAEQGMAQVSEETSLEVIKQYEPSKEGQLKACLSLDGFTNYLMSNDCHIFDPEQKTVCQDMNQPISHYYINSSHNTYLIEDQFRGPSDITGYIRALKMGCRSVELDVWDGPDNEPVIYTGHTMTSHIVFRSVIDIINKYAFVASEFPLILCLENHCSLKQQRVMFQHLKKILGDKIHSDPPRPEDNYLPSPNDLKGKILLKGKKLPNTCNSSEGEVTDEDEGAEMSQHMSIDGGEQQTVAQPKKFQLSKDLSDLVTLCKSIQFKDFPTAFQNQKHWELCSFNEVFASRCTTDYPGEFVNYNKKFLTRVYPSPMRIDSSNMNPQDFWKCGCQIVAMNYQTPGLMMDLNIGWFRQNGNCGYVLRPAIMREQVSYFSANTKDSVPGVSPQLLHIKIISGQNFPKPKGSGAKGDVVDPYVYVEIHGIPADCAEQRTKTVNQNGDNPLFDESFEFQINLPELAMVRFVVLDDDYIGDEFIGQYTIPFECLQPGFRHIPLQSLTGELLPHAWLFVHVAITNRRGGGKPHKRGLSVRKGKRNREYASMRILLIKALDEVFKTATQPLREATDLRENMQNAIVSFKELCGLSAVANLKQCILALSSRLLGADNNPVVLFNLKDQYPVVEAQGLLPDVLKKVVTAYETMIQTSRTLLENSESVYERILQVQKAAMEFHENLHDMAVKEGLKGRKLHKSVESFTWNITILKGQADLLKHARAEVLENLKQVHYAALTCNLAKGGPSTTPSTGSDSRPRRSLEAIPEKAVGEEDISDEEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JLD5</accession>
    <name>A0AAR2JLD5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570807.1">
        <property type="nucleotide sequence ID" value="XM_017715318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JLD5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080066.1">
        <property type="protein sequence ID" value="ENSPNAP00000052838.1"/>
        <property type="gene ID" value="ENSPNAG00000031864.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108437894"/>
    <dbReference type="GeneTree" id="ENSGT00940000163453"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000247">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000490">
        <property type="entry name" value="Zinc finger protein 605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF151">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="344" mass="40008" checksum="C0DC2B0845F60CBD" modified="2024-11-27" version="1">MLDVMWENGMTSSHRSSCGLQCPFLYTVLPPTYLQMEYVTLLLSREIKCENMESIISFGTQQTSSGAFYSNTPHGHLQESSGKEKTHHCLECGKSFTQRHHLQVHQRIHTGEKPHRCSECGKSFQKQNHLRIHLRVHTGEKPFECSECRKSFAVRSSLRRHQRVHTGEKPYQCSECGKSFSERSNLQTHERIHTGEKPYYCSECGKSFTTLNHLQLHQRIHTGERPYRCSDCGKSFTDGCNLKAHQRIHTGERPYNCSECGRSFTDRCNLKAHQRVHTGEKPYHCSECGKSFRQLNGLQKHQRIHTGEKPYYCSDCGRSFRYSLKRHKCTERERTKPQFSQMTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JQ11</accession>
    <name>A0AAR2JQ11_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JQ11"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055878.1">
        <property type="protein sequence ID" value="ENSPNAP00000051936.1"/>
        <property type="gene ID" value="ENSPNAG00000035797.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000278045"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="70" mass="8052" checksum="D38BB07231EC1492" modified="2024-11-27" version="1">MCLMSYVLYRCTSRFAVVSLSLGSRIEKTVVRNRFIETTPLIAITNKHITIDLGTGLVVSPKDYFTKYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JRD6</accession>
    <name>A0AAR2JRD6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JRD6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000062872.1">
        <property type="protein sequence ID" value="ENSPNAP00000054578.1"/>
        <property type="gene ID" value="ENSPNAG00000009913.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063460"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000133">
        <property type="entry name" value="FYN-binding protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043443">
        <property type="entry name" value="FYB1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029294">
        <property type="entry name" value="hSH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16830:SF12">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16830">
        <property type="entry name" value="SH2 CONTAINING ADAPTOR PRAM-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14603">
        <property type="entry name" value="hSH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="405"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="60"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="361"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="329"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="361"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="621" mass="68579" checksum="76CB100F5FB4AC17" modified="2024-11-27" version="1">MQRPFACLTSLFCSVPLTLWILALYLQDNKSDVKAIMARFQAGGTSMEVGAGGRPKVAVHPTLSGPTVPSKKPVLETSLSGSAAANSSTPKPSYLKNTVSTKSAPEARESLKPKALVSRFENTNTNTNEDSKPPFAKPLKPKLPDSSQDTEQKPQFPKAPLHKPTASSTAPDSKSPFPKPPPVTSKPSWTLKAENNGSSPNTSVTPKMPVSKPKPSAISSLLQQTDETKDADPLKKPFTSAKQSSFRAAQSVFNKSEEAAKDEAKSTSSKESPASNPTIAPKPSYCRKPSGSSIQSVNDDPSAPKKKPLPNILALGSPPSKPNRPPRVNLEKFKKAAESSNEGERRVFNSLLHMLRMSAMEAKEQDKKREKEEKKRVEQGKKDQKERERKEQEARKKFKLTGPLQVIHKVKARVDCKGGKNDLSIKQGEPIDILRITENPEGRWLARTQDGSYGYVKTESVEIDFDKLRNQGLSLPSHMDQDDGEVYDDVHIYSCIVVAGLNFFPSLPKLKAKAEAKTDDPKKQKKFEKEEKEFRKKFKFEGEIQVLYQVTAALSKKGSGKELAVQGGEVLDVIHKPEHDKLICRNKEGKCEDIDIYDDIGDGRSSKYLCFCRYSELDITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JWF9</accession>
    <name>A0AAR2JWF9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">EIF4A2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JWF9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051897.1">
        <property type="protein sequence ID" value="ENSPNAP00000056340.1"/>
        <property type="gene ID" value="ENSPNAG00000028563.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153783"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18046">
        <property type="entry name" value="DEADc_EIF4AII_EIF4AI_DDX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044728">
        <property type="entry name" value="EIF4A_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="84"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="266"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="427" mass="48166" checksum="B4493E31D738C1FD" modified="2024-11-27" version="1">LRASAPHSRHQTANTNPKRCKCIWGGWVGGGRAVCISPAFLLSVQSNWHEIVDSFDDMNLCEALLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQLDPELRATQALVLAPTRELAQQIQKVVMALGDYMGATCHACIGGTNVRNEVAKLQAEAPHIVVGTPGRVFDMLSRKFLSSKNIKMFVLDEADEMLSRGFKDQIYEIFQKLPSSTQVVLLSATMPVEVLEVTTKFMREPVRILVKKDELTLEGIRQFYINVEKEEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSALHGDMDQKDRDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTTVEEMPMNVADLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JY57</accession>
    <name>A0AAR2JY57_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TXNRD3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JY57"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059640.1">
        <property type="protein sequence ID" value="ENSPNAP00000056983.1"/>
        <property type="gene ID" value="ENSPNAG00000037247.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287919"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="291"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="357"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="440"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="499"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="529"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="562"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="594"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="624"/>
            <end position="644"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="650"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="681"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="705"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="738"/>
            <end position="765"/>
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        <location>
            <begin position="771"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="802"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="935"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="937"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="953"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="967" mass="96750" checksum="F7960412BE5D630B" modified="2024-11-27" version="1">PADPAHAPAAGPERGRTGETGVAVLYTVLTGGVLVLYTVLIGGVAVLYTVLIGGVAVLYTLLTGGVAVLYTVLTAGVAVLYTLLTGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTVLTGLTGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTGLIGGVAVLYTGLTGGVAVLYTVLIGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTGLTGGVAVLYTGLTGGVAVLYTVLTGGVAVLYTALIGGVAVLYTALTGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTVLIGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTVLIGGVAVLYTVLTDGVAVLYTVLIGGVAVLYTVLIGGVAVLYTVLTGGVAVLYTTGLIGGVAVLYTVLTGRVAVLYTVLTGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTVLIGGVAVLYTVLIGGVAVLYTGLIGGVAVLYTALTGGTGLTGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTGLTGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTVLIGGLAVPYTALTGGVAVLYTVLIGGVAVLYTVLTGGVAVLYTGLIGGLAVLYTVLTGGVAVLYTVLTGGVAVLDTVLTGGVAVLYTVLTDGVAVLYTVLIGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTVLTGGVAVLYTVLIGGVAVLYTVLIGGVAVLYTVLTGGVAVLYTVLTGGVAVPTFSIKPSQKSRGCYCSTDSLSTLSLQKKLWSLKASGRTALKYHTVLHITDAGAAQKIYVKKKITRFTQFYPGSTARSGKQSRLRLFNEQRLQQISLIKYNQRALRHVCFITEIKQVQNNKSEQKKEKGKNKDGVSLTKGTTSSLSIQLISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JZR0</accession>
    <name>A0AAR2JZR0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Macrophage-capping protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Actin regulatory protein CAP-G</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Interacts with NUP62. Interacts with NUTF2 and RAN; involved in CAPG nuclear import.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the villin/gelsolin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JZR0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057981.1">
        <property type="protein sequence ID" value="ENSPNAP00000055356.1"/>
        <property type="gene ID" value="ENSPNAG00000005816.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164612"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11289">
        <property type="entry name" value="gelsolin_S2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000002">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000004">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000005">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000040">
        <property type="entry name" value="macrophage-capping protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000037">
        <property type="entry name" value="macrophage-capping protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF27">
        <property type="entry name" value="SCINDERIN LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0117">Actin capping</keyword>
    <keyword evidence="13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="77"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="193"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="309"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="452"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="572"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="20">
        <location>
            <begin position="674"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000055356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="772" mass="85532" checksum="26233044288EE166" modified="2024-11-27" version="1">MAAGSFSLTARIQPCINMLVLLLSSVDDVSYFYCVFFITYIRVKYSRSPFIIMVTHKEFEAAGKEPGLMIWRIENMDLKPVPKNVYGSFYTGDAYLLLYTTKAPSYCIHMWLGDECSQDESGVAAIFSSQLDDYFGGKPVQFREVQNNESLTFLGYFKSGIQYKKGGVASGFHHVVTNEANVKRLLHVKGRRVIRATEVDFSWASFNKGDCFIIDLGKDIFHWRGSECNRFETVKASQVAIDIRDNERSGRAKLHMVEEGAEPQEIINVLGPITAIAPATPDDEKVDTSNKKKGVLYMISDATGSMKTSVVAQSSPFKQAMLSPDECYILDNGGDNNVFVWKGPNASVGERKAAMKVAQDFIKEKNYSSKTQIQVIPGGGETTLFKQFFSDWKDKDQTTGPSKAYTIGQIAVVKQIPFDASTLHSNKAMAAQHNMVDNGSGKVQIWRVEEGERVPVDPASYGQFYGGDCYIILYSYKQGSREQHLIYTWQGLKSTQDELAASAFLTVQLDDSMGGAPVQVRVTQGQEPPHLMSIFKGKPMIIHSGGTSRKGGQTQAGSTRLFHVRTSTAKATRAVEVQPSASKLNTNDVFVLKTPDSLFVWKGVGASAEELDAAKHVVTFLGGKPTDVAEGKEPAGFWTALGGKKEYQSSKTLQKTIKPPRLFGCSNKTGRLVVEEVPGDFTQLDLATDDVMLLDTWDQIFLWIGKDANEVEKTGAPKLAKEYVDSDPSGRRDIPITTIKQGAEPPTFTGWFQAWDAQMWDTDPLERMRAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K0S0</accession>
    <name>A0AAR2K0S0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Receptor-type tyrosine-protein phosphatase U</fullName>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Receptor-type protein-tyrosine phosphatase psi</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="RefSeq" id="XP_037396031.1">
        <property type="nucleotide sequence ID" value="XM_037540134.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000068121.1">
        <property type="protein sequence ID" value="ENSPNAP00000056102.1"/>
        <property type="gene ID" value="ENSPNAG00000021275.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411063"/>
    <dbReference type="CTD" id="563584"/>
    <dbReference type="GeneTree" id="ENSGT00940000157151"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000019">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000009">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000048">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000019">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000022">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000014">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF10">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE U-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 24" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043770242" description="Receptor-type tyrosine-protein phosphatase U" evidence="24">
        <location>
            <begin position="18"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="27">
        <location>
            <begin position="25"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="288"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="386"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="28">
        <location>
            <begin position="495"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="25">
        <location>
            <begin position="922"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="26">
        <location>
            <begin position="1073"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="25">
        <location>
            <begin position="1185"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="26">
        <location>
            <begin position="1364"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="640"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="842"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="842"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="864"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00083361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1455" mass="163603" checksum="A624160DBEFEC780" modified="2024-11-27" version="1">MMHALTLLLVLVLGVGGDNLEGPTAGCTFEEDPDVSLCEYRQGQEDDFDWQLVRTTSWPYVPSDLTRGSYMLVNSSQHGSGQRAQLLLRPLSENDTHCVQFSYFLYSRDGHSPGGLNVYVRVNGGPQGNSVWNISGSNGQEWHQVELAVSTFWPSEYQVVFEATVSAEQKGYIGLDDIVLLNYPCYKVPHFTRLGDVEVNAGQNATFQCVATGRAAKTDPFTMERRNGVLMTPHSLTSLVGERRLVAWFQVGGAQRGEQDLYRCITQSSYGAAVSNFAELIVRVPPTPIAPPQLLRSGPTYLIIQLNTNSIVGDGPVIRREIVYRASHSPWSETHGVNALTYKVWHLEPDTEYRISVLLTRPGEGGTGPPGPPLVSRTKCAEPMRPLRGLTASEIQSRQLALQWEPLGYNLTRCHTYSLSLCYRYSTATGGGAGGNNATVRECLSVERNTSRFTLRDLPPFRLVHVRLALSNPEGKKESREVTFQTEEDIPGGIAPESLTFTPLDDMIFLKWEEPVEPNGLITQYEISYQSIESFDPSVNVPGPRRTVSKLKNETYHMFSGLHPGTTYLVSVRARTAKGFGQTALTEITTNISAPAFDYDDIPSPLSESESTITVLLRPALGRGAPVSTYHVVVVEEDGSRQVKRRELGHQDCFPSPSSQGEAQGRSGGVPPHYYTAELPPSSLPEATPFTVGDNHTYNGYWNTPLDPSKNYLIYFQAASNFRGETRINCIRIARKAACKDHKHALEISQHAEDMGLILGACAGGLVVLILLLGAVVIIVKKGRDFYSYPYYPRKKVNMNKAAMTYRQEKNRKLGSLDCSTADQSTLQQDERTTHTFMDTHSCSARTEQRSSVNESSSLLGGSPRRHCRRKSSPYHTGQLHPAVRVADLLQHINQMKTSEGYGFKQEYESFFDGWDVTKKKDKTKGRHDTLLDHDRHRVKLHSLLADPNSDYVNANYIDGYQRSNHFIATQGPKQDMIYDFWRMVWQENCYSIVMITKLVEVGRMKCCKYWPDDSELYGDIKITLLKTETLAEYTVRTFAMERRGYPAKHEVCQFHFTSWPEHGVPYHATGLLAFLRRVKASTPPDAGPVVVHCSMGAGRTGCYIVLDVMLDMAECEGVVDIYNCVKTLCSRRINMIQTEEQYVFIHDAILEACLCGETAIPVSEFALTYKDMLRVDTQSNTSQLREEFQTLNSVTPHLDVEECSVSLLPRNREKNRSMDVLPPDRALAFLVSTESDGNNYINAALMDSFLRPAAFVVTPHPLPTTTGDFWRLVFDYGCTSIVMLNQLNQSNSAWPCLQYWPETGMQQFGPMTVELLSRSTDDDVITRLFRVNNITRLQEGHLVVRHFQFLRWSAYRDIPDSKKAFLTLLAQVQKWQRECGEGRTVVHCLNGGGRSGTFCACTMIMEMVRHHSMVDVFYAVKTLRNSKPNMVETMEQYRFCYDLALEYLDCLEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1P2</accession>
    <name>A0AAR2K1P2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB/POZ domain-containing protein 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1P2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066590.1">
        <property type="protein sequence ID" value="ENSPNAP00000056021.1"/>
        <property type="gene ID" value="ENSPNAG00000012920.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158298"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048512">
        <property type="term" value="P:circadian behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14822">
        <property type="entry name" value="BACK_BTBD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18287">
        <property type="entry name" value="BTB_POZ_BTBD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000005">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000038">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000051">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000042">
        <property type="entry name" value="BTB/POZ domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052407">
        <property type="entry name" value="BTB_POZ_domain_cont_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034091">
        <property type="entry name" value="BTBD9_BACK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46306:SF1">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="108"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="636"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="686" mass="76646" checksum="CAE9036FABAD2357" modified="2024-11-27" version="1">MGWVTAFQCHQDCLLGGKTETPNINMTEFCIITVGIRVWMNSLTENASCGKMLAKLIGKHKHSDGTAAAAVMSSSSHPLRPLGSVSEIDHVDLLSEQLGALIPGEEYSDVTFVVEQKRFPAHRVILAARCHYFRALLYGGMKESQPQAEVCLEETRAEAFSMLLGYIYTGRVSLSAAREEVLLDFLGLAHRYGLQLLEDSTSEFLRTILNTHNVCLVFDVATLYGLSALSSACCAYMDRHAPEVLASDGFLSLSKAALLTVVCRDSFAASEKEIFQALCRWCRQNGEAEATQEVMSMVRLPLMTLTEMLNVVRPSGLVSPDDLLDAIKLRSESRDMDLNYRGMLIPEENIATMKYGAQVVKGELKSALLDGDTQNYDLDHGFSRHPIEEDGRAGIQVKLGQAFIINHIRILLWDRDSRSYSYYIEVSMDELDWVRVVDHSKFLCRSWQNLYFPPRVCRYVRIVGTHNTVNKVFHLVAFECMFTQHPFTLEKGLLVATENVATVSACASVIEGVSRSRNALLNGDTRNYDWDSGYTCHQLGSGAIVIQLAQPYIIDSLRLLLWDCDERSYSYYIEVSTDQQHWIKVVDRTKVACRSWQTLKFDKHAASFVRIVGTHNTANEVFHCVHFECPAQLDTEVKEGSPGNSWPEAGTSTQQLRPPRPARSHSLLPSQTPSSSSSSSSSPAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1V5</accession>
    <name>A0AAR2K1V5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase III subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC7 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1V5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070498.1">
        <property type="protein sequence ID" value="ENSPNAP00000056424.1"/>
        <property type="gene ID" value="ENSPNAG00000031174.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000288914"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024661">
        <property type="entry name" value="RNA_pol_III_Rpc31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="137"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22383" checksum="A24255C0104DB570" modified="2024-11-27" version="1">MAGRGGKGRGIAAFTFNIEALGLSRASMPETQRGPQPMFPVSLEFKPVPLKAGEDEDYMLALKQEIRGKMKGLPFHIKPPSGKNGEWCALVDLITVMLLLLLLLLLLLLLLLFRHFIPKQINFCCIWSVLKELEKRGDDKSDEEREEKKKGEEEEEEEEVGEYDEELEEENDYIANYFEDGDDYGAGSDDNMDEATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K2B6</accession>
    <name>A0AAR2K2B6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ankyrin repeat and IBR domain-containing protein 1</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="RefSeq" id="XP_037392904.1">
        <property type="nucleotide sequence ID" value="XM_037537007.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037392905.1">
        <property type="nucleotide sequence ID" value="XM_037537008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K2B6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076943.1">
        <property type="protein sequence ID" value="ENSPNAP00000056584.1"/>
        <property type="gene ID" value="ENSPNAG00000011136.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411961"/>
    <dbReference type="CTD" id="561277"/>
    <dbReference type="GeneTree" id="ENSGT00940000157621"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20361">
        <property type="entry name" value="Rcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16774">
        <property type="entry name" value="RING-HC_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000003">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000040">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000129">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047564">
        <property type="entry name" value="Rcat_RBR_ANKIB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047563">
        <property type="entry name" value="RING-HC_RBR_ANKIB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="330"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="334"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="772"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="969"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1013"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1033"/>
            <end position="1047"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1066" mass="119858" checksum="A93E383833FB1F59" modified="2024-11-27" version="1">MGNTATKFRKALINGDEVLAYQLYEGNPQFKESLDPNTSYGEPYQHNTPLHYAARHAMTRILRSFLFSKDGNPNKRNVHNETALHMLCMGPHILLPEGALQPRLARPYEDERRRADCLQMILKWTGAKLDRGEYESADVNATDNKKNTPLHYAAASGMKTCVELLVTRNADLFAENENKETPCDCAEKQHHKELALSLESQMVFSTDPNAEDIEAEYAALDRREFYEGLRVQDLRRLKDMLIVETADMLQAPLFTAEALLRAHDWDREKLLEAWMSNAEDCCQRSGVLMPTPPPRGYNAWDTLPSPRTPRTTRSSITSPDEISLSPADDDRSLCGICMCTASMFEEPVDIPCGHVFCRTCWESFLNLKIQEGEAHNIFCPAYDCFQLVPVEVIESVVSREMDKRYLQFDIKAFVENNPAIRWCPRAGCDRAVRLAGQGPGASASDPLSFPLLQAPAVDCGKGHLFCWECRGEAHEPCDCQTWKMWLQKVSDMKPEELAGVSEAYEDAANCLWLLTNSKPCANCKSPIQKNEGCNHMQCAKCKYDFCWICLEEWKKHSSSTGGYYRCTRYEVIQQVEEQSKEMTVEAEKKHKSYQELDRFMHYYTRFKNHEHSYELEDRLLKTAKDKMEQLSKVLSGREGGPPDTTFIEDAVHELLKTRRILKCSYPYGFFLEPKSTKKEIFELMQTDLEMVTEDLAQKVNRPYLRTPRHKIIRAACLVQQKRQEFLASVARGVAPNDSPEAPRRSFAGGTWDWEYLGFASPEEYAEFQYRRRHRQRRRGDMTSLRSNTPDSDDHNPGTLDSEQGGCSQRPGLPMPHSSLDEDDPNILLAIQLSLQESGLTAGAGGGMVDAQELLAHEASMGAIGTSLPSRLDPLLHGVDVPRAALSSSELLEVGDSLKKLGNMSGQNVPQRFNNLGNPNPVGVGVPFPPLPGQMETTDMDPADNANLLGNIMAWFHDMNPQNISLVPPVAPSDSEFSTQAPEQPVANPFQQWPPEGDREVCSVEPVRPTQLDLVSVDVSSAPPLVPQSSEEQETSHPCHSETPKCDSDPEQPSSSSSEWEGQVHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K6D7</accession>
    <name>A0AAR2K6D7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pleckstrin homology domain containing, family G (with RhoGef domain) member 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K6D7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000041851.1">
        <property type="protein sequence ID" value="ENSPNAP00000058054.1"/>
        <property type="gene ID" value="ENSPNAG00000035406.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161250"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040181">
        <property type="entry name" value="PKHG5/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217:SF10">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 6 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13217">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="208"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="527"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="595"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000058054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="887" mass="101206" checksum="20E3C20F5B082AEC" modified="2024-11-27" version="1">MKVTPTQLFSNLPNILNAHKLFWQEVMYPMLKNVRLTGQPFDPLQLEPGCLQFAERFPDYVEYCLEEERNVEFTRRQLETNSHFHAYLMWVETHPQCMRMRLGDMQAKPHQRITKYPLLLKAILKTTQDPHTQHALRCMLAGVGEFLDSINDHLQFKDDELALSVHSQKIEGYEVQGFNEEIDKYIQEFCHFNLKSPIRGIGPKVIRKLLLEETLKVKGRKDSKLEVVVLLFTDVLLLTKTQKKSEKHKVVRPPLSLERIRCAELKDGYSFVLVEVSDLGCAISVYSVSAPSTESCTAWVSAIHQAQEALETLRKAQNQSKPKMPSDQVELEESETLPFPSTQSTEDRKEQPEAQAEFTNDSSQIAPGLKLTANHLESREDPQVGSKVQNSHSQAARASSTSKQMQNRDKEFTDEQTQDSEEKQRSESRHQEVDDVLSSVINERRVTWSHITTKSSDTSNEGLKTNKSNDPHPLLVGGLMENTTPSQISSSKTVLQSGHKPAEPAINNAKHRAFTSVERAKQQNLNDQSQESDSCQESLQSGEEGEVLSESSRFSRKLKSPRLRRKRPMNSQSYAPTQGSRRMSDGAESVRLLCDRNSPSNSDSDGNNSLRRPSNSSSEPQDAHLVLKLASLKRISLDLHAFSEPELFNKMPQNSLKIPQMKSQRSASTPDVYLNTQSFPLCIPNRLCPSPPPGLDPQLHPSPLQGLLDRAKERDRERGLAKRDGKPTEKNVNTLQNRIYISNSPSSSHNEGERETAGEEQDLFRQQGKPYLQIECSVDGPENERRYSPVTPLRVSVDWPGWCFDDEELREFWKPYDGTIDWWDHAVTTEELQQTLRHEDGEYSEVDNGLHSWILHDHFSLQGWMERKMRGGQMIVRAFFVLYSMYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K6H5</accession>
    <name>A0AAR2K6H5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">28S rRNA (uridine-N(3))-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Centromere protein 32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Kinetochore-associated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">SPOUT domain-containing methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SPOUT1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uridine in 28S rRNA + S-adenosyl-L-methionine = N(3)-methyluridine in 28S rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:83635"/>
            <dbReference type="Rhea" id="RHEA-COMP:20178"/>
            <dbReference type="Rhea" id="RHEA-COMP:20181"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:83636"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with INCA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580710.1">
        <property type="nucleotide sequence ID" value="XM_017725221.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K6H5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000059401.1">
        <property type="protein sequence ID" value="ENSPNAP00000057711.1"/>
        <property type="gene ID" value="ENSPNAG00000016265.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444198"/>
    <dbReference type="CTD" id="51490"/>
    <dbReference type="GeneTree" id="ENSGT00390000016537"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031616">
        <property type="term" value="C:spindle pole centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051661">
        <property type="term" value="P:maintenance of centrosome location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035196">
        <property type="term" value="P:miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000170">
        <property type="entry name" value="SPOUT domain containing methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="380" mass="42824" checksum="407A14ED90650E3B" modified="2024-11-27" version="1">MSEFPARKKQKPSSGEAQERVDWRKRKAEKKEQKRKWKEAKLIEQLEKQNQAKAEEEKMQQQCQEEEEPKAEGRLYTVSIALPGSVLDNAQSPELRTYLAGQIARACAVFCVDEVVIFDEQGEDTRTVQGEFKGVGKKGQACVQLARILQYLECPQYLRKAFFPKHSDLEFAGVLNPLDSPHHMRIDEEMEFREGVVLDRPCKARKGSFVNCGMRKEVQIDKQLQPGLRVTVRLLHKNQKPESRVHCGVVVAPHVPRTEGGLYWGYSVRLASCLSAVMTECPFKDGYDLTIGTSERGSDADPTPLPPFRHMLVVFGGLKGLEESVATDPNLDVTDPSVLFDLYLNTCPGQGSRTIRTEEAILITLSSLRPKIATSDHNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K7Y1</accession>
    <name>A0AAR2K7Y1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Versican core protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Chondroitin sulfate proteoglycan core protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Large fibroblast proteoglycan</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">PG-M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">VCAN</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Interphotoreceptor matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aggrecan/versican proteoglycan family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000048262.1">
        <property type="protein sequence ID" value="ENSPNAP00000058206.1"/>
        <property type="gene ID" value="ENSPNAG00000008733.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156102"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033165">
        <property type="term" value="C:interphotoreceptor matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
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        <property type="entry name" value="CTDL_fold"/>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig/MHC_CS"/>
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        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
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        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
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        <property type="entry name" value="VERSICAN CORE PROTEIN"/>
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    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 27" id="KW-0732">Signal</keyword>
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            <begin position="256"/>
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            <begin position="1114"/>
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    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="512"/>
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    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="734"/>
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    </feature>
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        <location>
            <begin position="517"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="672"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
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        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="24">
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="10">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
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    <evidence type="ECO:0000256" key="24">
        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50041"/>
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    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
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    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
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    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1329" mass="147982" checksum="E5CF4E51B195CE3C" modified="2024-11-27" version="1">MEMLVGIKHIFWFVSLCSAVTHEDSLQWMRVEKSPPVWGFLAEKVVLPCHFSTLSTSVPLPNSALTTTITLNHLRIKWTKLEGNTEATVLVAQNGVIKIGPDYRGRVSVPSHPEDVGDASLTVVKLRASDAGVYRCEVMHGIEDTQDTVILDVFGVVFHYRASTSRYNFNFERAKQACKDAGATIATVEQLTAAYEDGFEQCDAGWLADQTVRYPITRPREGCHGDKIGKPGIRTYGRRDPTETYDVYCYVDKLKGEVFYPPISTKFTLQEAKKECEKFGAVLASPGQLHAAWREGLDRCDFGWLSDGSARYPISVPRMQCGRGLLGVRTMYRFINQTEYPLPTQKLGAFCFKGQDPTIDPTEILQTSLPTVAIASMASTTQFIDYDVDNFSQSINQVHAVPDRRVPHLTQLHPLPTMKSISPQLDITADISSTEAGIRGETDISERVTQQMTVVQEPSISKQDTNKLAVVYKKPKEGSTKTIAIKALADETTISMTEETLVTAESAIRIREEEGKTANTEDFSTQPVFESPLSGTEGNRSASAPPINVIVIDMNNNVSADHIAGIIGEGIPLDSNPVLTFINGKHELNLKAETEETLEARGDQFETASPIVSEEKLEEIEVITSSAVVTELSSPESNLHDTVTTSEIPQILTSKILLDETSVYDSISKTSTFDEGHPKDDELPSPVDSAYNALPKTASYSQEEVVTDESEIPEFKFPTKTPFMEYAITTSHSTFHRERQSYPDDYEGSTSSAEDGSAQYQYSLKGSKPSPPPAFTLSSLLSTVSSKPIDSTPLSQTVTMISPNTEGKMFSISKENAMPGWVESDTKTEVDDVKGTEIPLSVPESEMVKSIVFSITEYENHSEQPTETFSRETTAQLHFIHQIAASENTFTDAAIRKQFEGHTRSETVSKLITQSPPTTPVSEDYTSEVTVLTASFSKFSTEQVLISTPEHAQKHVKVVTHHFTTEESLTSPVQATTAMPFEEDLDDTGSLSIFAVPPHKKEVTKVEPHAGAHNVTTVVEHTVDLPDFTLCAVNICANGGTCFFNGKSNICLCVPGFTGDHCENDIDECQSNPCRNGATCIDGLNSFTCVCLPSYSGALCEQDTETCDFGWHKFQSHCYKYFTHRRTWDAAERECRLHGAHLASVLSHEEQLFVNRLGHDYQWIGLNDKMFENDFHWTDGSAMQYENWRQGQPDSFFSTGEDCVVMIWHEAGQWNDVPCNYHLTFICKKGTVACSQPPIVKDAHIFGSIKPRYEINSLVRYHCKDGFIQRHVPTIRCRTDGHWDKPKIICIQSSTYQKTYSQYYQANNKERYNGWVQQNWGEKGEQTRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KHT4</accession>
    <name>A0AAR2KHT4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with GGCX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CREC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KHT4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000065203.1">
        <property type="protein sequence ID" value="ENSPNAP00000063888.1"/>
        <property type="gene ID" value="ENSPNAG00000025643.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222360"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000090">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000109">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000110">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF87">
        <property type="entry name" value="CALUMENIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043792775" description="EF-hand domain-containing protein" evidence="18">
        <location>
            <begin position="20"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="265"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="316" mass="37467" checksum="BFB395A5AA4FD532" modified="2024-11-27" version="1">MELWPLLLCASLCVVHIYSKPMEKKDRVHHDAPLSDKEHDDDDNFDYDHDAFLGQEEAKTFDQLTPEESKERLGIIVDKIDEDQSGYVTVEELKKWIKHAQKRWIYEDVDRQWQSHDLNGDSFVSWEEYKNATYGYILDESDPEDGFNYRQMMFRDERRFKMADKNGDLQADKEEFTAFLHPEEYDYMKNIVVLETMEDIDKNGDGFIDLEEYIGDMYSQNGDPTEPEWVRTEREQFTEFRDKNKDGRMDKEETKDWILPSDYDHAEAEAKHLLYESDADKDGQLTKQEIVNKYDLFVGSQATDFGEALVRHEELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJ45</accession>
    <name>A0AAR2KJ45_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Latent transforming growth factor beta binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LTBP2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the LTBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSPNAT00000045410.1">
        <property type="protein sequence ID" value="ENSPNAP00000062479.1"/>
        <property type="gene ID" value="ENSPNAG00000010267.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155823"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000068">
        <property type="entry name" value="Latent transforming growth factor beta binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000205">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000019">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000046">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1 isoform x2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000001">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000056">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000115">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000024">
        <property type="entry name" value="Putative latent-transforming growth factor beta-binding protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000273">
        <property type="entry name" value="Putative latent-transforming growth factor beta-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF4">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036312">
        <property type="entry name" value="Fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043378081" description="Latent transforming growth factor beta binding protein 2" evidence="13">
        <location>
            <begin position="22"/>
            <end position="1862"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="84"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="307"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="15">
        <location>
            <begin position="458"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="528"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="15">
        <location>
            <begin position="578"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="832"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="875"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="955"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="996"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="1205"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="1244"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="1327"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="15">
        <location>
            <begin position="1439"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="1559"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="15">
        <location>
            <begin position="1616"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="1805"/>
            <end position="1849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="129"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="405"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="666"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1672"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="188"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="666"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="683"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000062479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1862" mass="202831" checksum="893A614447084386" modified="2024-11-27" version="1">MERLWFLLLVSLSFSLTAASGQLLVRSPRQLRMVGRGRSSAKQSPPDHQESKTTHPRKLVGPHVCGGQQCCSGWAIAPGTNRCIKPDCQPPCQNRGSCSRPHTCVCRSGFQGERCEEVAPEQVYIRTGTSRVLTPVRPGSESSQRQPGRRRPTERQAIDPVRTQTPKPPTTRVPHSPITQVRRGSPDISPQQTGTSRTVKRYPSSSGPITSNALPSSNGNGFSSHGYSHTQHRSSSSSQLHDTHSNSVSAAGANLTTGMDRIKIVFTPMVCRRTCNGGRCYNSCERGDKTTIYSKSQGPEPKSQGFRLFFCQLPCLNGGRCIGQNECWCPSNSTGKFCHLPAPTPSKPTPSRKANSNGVQNGSSHSMYTLPLSNQQASMLPSLVNVHVQHPPEAEVQIHQVARVKPGQTADGAHSVQQRSQPGNSNGNGSGHYTVHSYGQNGGHSHHGPHHRLNGYVGRCFQETIDGQCGKPLPGLTKQEDCCGSVGASWGLNKCTKCPSKPAYAVIANGQVECPKGYKRMNSTHCQDINECLMPGICKHAECLNTKGSYRCTCKPGYMLDPARSHCVSDKAVSMEKASCYRSVSAGSCGLPLSLKITKQICCCSRVGKAWGSHCERCPLPNTDLFKEICPAGHGYTYSRSDIQITLRELDEEEISTVESFLSQIPTYSQPEQTPPETSDPLPQLPISPQPPYYHPQAPTRPQPPKTPQPGQPPRTAQVPHHPYTDPQHHVHVSPSLPEDETLDVQPTFTASPPSYPDVVEVTSHMPTQLKPESDGLDTAPINVATQATDIDKCSLTPTICGPGQCVSVQTGYTCYCDPGYKLNGLQTTCIDVNECEDDPCGGKGHCVNTYGSYTCQCFSGFSLLMTQNKKTCQDVNECAIPNKCPNGRCINTEGSFTCECKSGFTQNWRGQCEDVDECRSPSVCPGGICVNTQGSFQCQACGPGFRPAGGRCVDINECQTAGACANGRCVNTEGSFICTCNHGYQTTLRNDSCQDVNECLVPDICPHGTCVNLPGSHRCNCNTGYQTSPDGKACEDVDECAHPSLCVEGVCVNTVGYFTCTQCEVGYTLSEDRQRCEDIDECLSLSTCPNGICLNSDGSFSCVDCPTGYSVSADGERCDDIDECALPNACPQGKCTNTEGSYTCTICGPGYRISDDGSHCEDIDECQSPEVCPQAGCMNTEGSYSCMTCGPGYKLSSDRRTCEDVNECESMAMCLHGDCINIAGSYHCDCHIGFELRDADCRDVDECGVAGTCTNGQCMNTDGSYQCLCESGYEFSEDTKECEDLDECKSYSDVCGTWNCENTLGSYRCFMGCQSGFYGEENKDCDIDECEDETICGKHGFCENTDGSYRCQCDRGYTSSPERQICVDVNECETHSDLCGEALCENAEGSFLCICPNENEEFDPVTSQCSQHSTDLPDSAGPVFQAPPPVASDEGQRKECYYNLNDANLCENVLSRNVTKQECCCTVGAGWGDNCEIHPCPIKGHEEYDWLCPHGSGILTVISSVQGFSQLQLRDADECEMFGPEICKNGHCSNFYSTYSCFCRSGYYYDHIRMECVDYDECKEENLCEHGLCVNTPGSYNCFCSPPLVLDSTQRHCISINITDGAHEFHNVHVDICWQELKADRMCSHPMLDTRTTYTECCCVSGVAWGSQCALCPGQTSSEFAELCNQPQRGSDSLRERPGFEYGPDASPDGPYWDSYGHLGGDPYYIPEGPLNGPIFPDPSSDYSSREAVQVPVHRTRENQPPRRVDPYMGHNEGFEGLQAEECGILNGCENGRCVRVREGYTCDCFDGYELDLSKMACIDINECKDISDKVELCKNGVCSNTEGSYTCTCLPGFLATSKPHECIAEDQASAPHTEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KJQ5</accession>
    <name>A0AAR2KJQ5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017560208.1">
        <property type="nucleotide sequence ID" value="XM_017704719.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017560209.1">
        <property type="nucleotide sequence ID" value="XM_017704720.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037388037.1">
        <property type="nucleotide sequence ID" value="XM_037532140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KJQ5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055310.1">
        <property type="protein sequence ID" value="ENSPNAP00000064510.1"/>
        <property type="gene ID" value="ENSPNAG00000030331.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108431513"/>
    <dbReference type="CTD" id="100008047"/>
    <dbReference type="GeneTree" id="ENSGT00940000157869"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18394">
        <property type="entry name" value="BTB_POZ_KCTD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF10">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="12"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000064510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="237" mass="27738" checksum="AFB7311292C27361" modified="2024-11-27" version="1">MENGDWGYMMTDPVTLNVGGQIYTTSLSTLQRYPDSMLGAMFRGDFPTARDTQGNYFIDRDGPLFRYILNFLRTSELTLPYDFKETELLRKEADFYQIEPLIQCLSDTKPLYPLDTFEQVVELSSTRKLSKYSNPVAVIITQLTITTKVQTLLEGISNNFTRWNKHMMDTRDFQVSFTFGPCDYHQEVSLRVHLVEYISKQGFTIRNTRVHHMSERANENTVEHHWTFCRLARKVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KSC1</accession>
    <name>A0AAR2KSC1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U2 snRNP-associated SURP domain containing</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">U2SURP</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP DOMAIN-CONTAINING"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="196"/>
            <end position="277"/>
        </location>
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    <feature type="domain" description="CID" evidence="5">
        <location>
            <begin position="432"/>
            <end position="577"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="141"/>
            <end position="201"/>
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            <begin position="690"/>
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        <location>
            <begin position="762"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="695"/>
            <end position="714"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="716"/>
            <end position="727"/>
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            <begin position="860"/>
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            <dbReference type="ARBA" id="ARBA00022884"/>
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    <sequence length="936" mass="107060" checksum="25552EADBD38BC53" modified="2025-10-08" version="2">MSDALLESKLKAFSIGKMAVAKRTLSKKEQDELKKKEDEKAAAEIYEEFLAAFEGGEGRVKTFVRGGIANASKEEAASDDKRGKLYKPKSRILEAKSFLPLETPPQFLAFDKRNASKKGEKEKKKSNLELFKEELKQIQEERDERHRLKGRVSRFEPVSAMEGRRSSDGSSRRNRPSSVLDDSAPGSHDVGDPSTTNLYLGNINPQMNEEMLCQEFGRYGPLASVKIMWPRTDEERARERNCGFVAFMTRRDAERALKHLNGKMIMNFEMKLGWGKGVPIPPHPIYIPPSMMEHTLPPPPSGLPFNAQPKERLKNPNAPMPPPPKNKEEFEKTLSQAIVKVVIPTERNLLCLIHRMIEFVVREGPMFEAMIMNREINNPLYRQVSVMLLKSLFVWVYKWFITVVIPLKEKIWVPIDFQCLLIFEPSHFNPVSRCRERDKLEEVLRGLTPRRSDVAEAMLFCLTHADAAEEIVECIAESLSILKTPLPKKIARLYLVSDVLYNSSAKVSNASYYRKFFEAKLCQIFSDLNATFKTIQGHLQSEHFKQRVMSCFRAWEDWAVYPDPFLIKLQNIFLGLVSLDPEKETTTEVLPEQPTEQVEDIDGAPIVEEELDGAPLEDVDGTPIDGAPLDGAPMMDDLDGVPIKGTEDELDGVPLDGPKPAFKMAPSKWEEVDGAALEAQAVTTSKWEIFDLPEESEKTNESRKTHETEVKETLKSVYSTEQPSHSQPIKEEYQDSKSAKLSEMSEEKRAKLREIELKVMKFQDELESGKRPKKSGQSIQEQVELYRDKLLQREKEKEVENKEKEKEKEKKEKDKAEVMHKEKEKEESTSNRKEKKRRHSPSPSPTRSSSSRRGRSPSPRSERSDRSYTKDSSRSSYKDSPRDSSRKSSKRSPSPPVTPKRSRRSRSRTPKKSSKKSRSRSRSPHRSHKKSKKSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KV83</accession>
    <name>A0AAR2KV83_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="14">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-specific succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="14">A-SCS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Succinyl-CoA synthetase beta-A chain</fullName>
            <shortName evidence="14">SCS-betaA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 18" type="primary">SUCLA2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 14">ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the succinate/malate CoA ligase beta subunit family. ATP-specific subunit beta subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2KV83"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073508.1">
        <property type="protein sequence ID" value="ENSPNAP00000066389.1"/>
        <property type="gene ID" value="ENSPNAG00000018855.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010170"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000001">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000040">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03220">
        <property type="entry name" value="Succ_CoA_betaA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034723">
        <property type="entry name" value="Succ_CoA_betaA_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF1">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 14" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp fold succinyl-CoA synthetase-type" evidence="17">
        <location>
            <begin position="60"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="16">
        <location>
            <begin position="334"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="393"/>
            <end position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="14">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="14">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000066389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="476" mass="51484" checksum="7BDCF35B7D8E3C91" modified="2024-11-27" version="1">SSAVGVFKHLCVTAGLRIVHRPKISSQQRPVLAGTSGLFGNHGNQLSPQATLQQQRNLSLHEYMSIGLLREAGISVPAGTVASTPEEAYAAAKQLGSKDLVVKAQVLAGGRGKGTFEGGLKGGVKIVYSPEEARDISSQMIGRKLYTKQTGEAGRICNQVFICERRYPRREYYFAITMERSYQGPVLIGSSQGGVNIEDVAAENPDAIVKEPIDIVEGIKKDQAVNVAKKMGFPDSLVNEAAEMMMKLYDVFIKYDASMVEINPMVEDSSGVGMQTHFIIHINQAHYINFDSNAAYRQQKVFSMRDWSQEDTRDRQAAKADLNYIGLDGTIGCLVNGAGLAMATMDIIKLHGGTPANFLDVGGGATAHQVTEAFKLITSDRKVQAILVNIFGGIMRCDVIAQGIIMAVSDLDLKIPIVVRLQGTRVDDAKALIAASPLKILACDDLDEAAKMVVKLSEIVSLAKEAQVDVKFQLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KWS4</accession>
    <name>A0AAR2KWS4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NTSR1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017556449.1">
        <property type="nucleotide sequence ID" value="XM_017700960.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KWS4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047872.1">
        <property type="protein sequence ID" value="ENSPNAP00000068798.1"/>
        <property type="gene ID" value="ENSPNAG00000036591.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108429297"/>
    <dbReference type="CTD" id="4923"/>
    <dbReference type="GeneTree" id="ENSGT01120000271823"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016492">
        <property type="term" value="F:G protein-coupled neurotensin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15355">
        <property type="entry name" value="7tmA_NTSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003985">
        <property type="entry name" value="NT1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003984">
        <property type="entry name" value="NT_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF9">
        <property type="entry name" value="NEUROTENSIN RECEPTOR TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01479">
        <property type="entry name" value="NEUROTENSINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01480">
        <property type="entry name" value="NEUROTENSN1R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="64"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000068798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="394" mass="44277" checksum="0FCA26C13F54F601" modified="2024-11-27" version="1">MDANGTLNFSKLRALTQQLLANHSAPFHANATAPQEDLDVNTDVYSKALVTVIYVLLFAVGCLGNSITLYTLLTKKSLQNLQSTVHYHLASLAVSDLLILVLSMPIELYNFIWVHHPWAFGEAVCKGYYFLRDGCSYATAFNIASLSVERYMAICHPFKAKSAMSRSRTKKLISGMWAAAFLLATPMLFTMGERTVDGEPICTTIVSTATAKSVLQVNAFLSFIAPMVLISVLNGIIASQLLRMFREAAQDNRICVVGGNATMLSVAVEPNRAQSLRHGVMVLRAVVIAFVVCWLPYHGRRLMYCYVTDWTDTLYDFYHYFYMVTNVLFYVSSAVNPVLYNLVSANYRQIFFSTLSYFCLPCRRKKQTRMLTRHSISICSNQTFSTNIVKETVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L5Z4</accession>
    <name>A0AAR2L5Z4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L5Z4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000047042.1">
        <property type="protein sequence ID" value="ENSPNAP00000069796.1"/>
        <property type="gene ID" value="ENSPNAG00000032164.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272338"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="132"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="236" mass="27188" checksum="A900E6B46A8A51C1" modified="2024-11-27" version="1">MQYFAIKCSFSCSYNEFLYSSYVSGAFGQDWSVKYTQTRKCALKGFTVYMSGDYRYPSHLEGVKRFWAINPGRKVEPVDLTTDPEYSGRTEYFEGRNKLFYLKLMNVTERDEHMYSIRITTNIESQRYLFYPGVTLNVTGLRVESPERVSEGNTTRLTCTTTCPLPDRPTYIWYKNGRRVDRNDEETNVLSLRPTRIEDTGSYSCAVRGYEGLPSPAAALSVRCEYLLSAGQIYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LEB9</accession>
    <name>A0AAR2LEB9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">P4HA2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LEB9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060041.1">
        <property type="protein sequence ID" value="ENSPNAP00000074943.1"/>
        <property type="gene ID" value="ENSPNAG00000014592.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157695"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000006">
        <property type="entry name" value="Prolyl 4-hydroxylase subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000001">
        <property type="entry name" value="Prolyl 4-hydroxylase subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059068">
        <property type="entry name" value="TPR_P4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23558">
        <property type="entry name" value="TPR_P4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="9" id="KW-0847">Vitamin C</keyword>
    <feature type="repeat" description="TPR" evidence="14">
        <location>
            <begin position="232"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="15">
        <location>
            <begin position="439"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="562" mass="65242" checksum="560091D08D1E4676" modified="2024-11-27" version="1">MYFSSHQLDSGLKQNSKDFLKYTQFKEKILFIWYQTWLRCFLFESFWLETFFIRMTDLIFIEREVVQSLKEYIQAEESKLAAVKRWANKLEVLTHASTSDPEGFLAHPVNAYKLMKRLNTEWSELESLVLNDPSHGFISNISVHRQYFPDEEDEKGAAKALMRLQDTYNLDSESFSKGKLPGVRYNAFLTVDDCFDMGKTAYNEQDYYHAVLWMQQALRQMDAGEEAKASKADILDYLSYSVYQMGDLQRATELTRRLVSIDPGHQRAGGNLRYFERLLAKEIREVARNKQDPAKEKPIQLGTYERPRDYLPEREIYEALCRGEGVKMTPKRRSRLFCRYQDGNRNPRLLLKPMKEEDEWDNPHIVRYLEALSNEEIEKIKELAKPRLARATVRDPKTGVLTTAHYRVSKSAWLEGDEDPVIDRVNQRIEDVTGLTVDTAELLQVANYGVGGQYEPHFDFSRKDEPDAFKSLGTGNRVATYLNYMSDVEAGGATVFPDFGAVIWPRKGTAVFWYNLFRSGEGDYRTRHAACPVLVGSKWVSNKWIHERGQEFRRPCGLTEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LIX5</accession>
    <name>A0AAR2LIX5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centrobin, centriole duplication and spindle assembly protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LIX5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048464.1">
        <property type="protein sequence ID" value="ENSPNAP00000074291.1"/>
        <property type="gene ID" value="ENSPNAG00000036286.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00610000086191"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051299">
        <property type="term" value="P:centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902410">
        <property type="term" value="P:mitotic cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038923">
        <property type="entry name" value="Centrobin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34439">
        <property type="entry name" value="CENTROBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34439:SF1">
        <property type="entry name" value="CENTROBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="349"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="757"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="841"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1053"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="541"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="766"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="808"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="841"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1064"/>
            <end position="1074"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1083" mass="120737" checksum="EF91501F96E935E4" modified="2024-11-27" version="1">MLSAPHYPCTSVITRLSLCVPQCEMSVETADVLSDVEPLPVSSPASPSPPLFPLSSSFPSLTRSWPSSPLSSSRQVTARLYSSLQRSKEQELYSTGGYTARRPLVSTDHSAKARQVSFKPSSPELGSLAVATGSVPSDRLNESQQADLSLEADSSRDAASGAEETESEAELMTGEMDLKLQSSVNSSAKLSSGRRHIEEMENVRSHLQTMLRSVPTATDTDDLLRPASQHLQDDSYKSDTTSHLLSAALSLGGVEELFPRYSRLRAGVNPAPLPSVSELQVIRESLDRERARRKHSEQQVLVLQNKLLALQQQLALAVSADRKKDIMIEQLDKTLEKVVEGWRRHEQEKSEGVRRLQEEKEAAESARDKHREALASFEKSLSEAAEALDKEQKRSEELQNVNKQQERELAELRGCVEELRVRVEERRSEAEEERAKSEKLQTLAVTLQTELDQQNQQSRHSLSQLQQDITRLTQQLDTERERVGQEVQLREEAQSTVRQLQQELEETRRERDTARVDRALDQARFEAQRSQWEVELRLCVEQQVTERLANIQQENSTATAKLREQHRKQLLDLSARQERELSAQMEEFRVQLEEKDEKQQQLALHFNNKMAALQEEMVSMEASKRRLETQREELVSRLQGMMRSHWTEALRLLNNQEQADGMFSPMSLWDGSKPHSSPHDPDSSICSTANTLASAAPRAVVLHLSRERERGMKGEEEVRVERGRGESDFGLLDHSHVFCPLEPVLDDTDLTAVGGSNLTGLWDRPGGGEKQREAGRERTEREGGRTGIRGQAVEMGPTVRSHGESPGQALNQTSNHFRNQESYSDSDRIRSQFEQCVNRQANQNPDQSARLNQVHASSERLTSQSYSSEKGAGRCDVSVEKYSGAGMKAPPMKEQSLSIKERAPPTGTSGSSLGEDRQNELQYYISKLLDRSPGDPLEEQPVEQRKAETLPVNPGVNSQWDGFSSGPRALVNSLLTRAPAPPQAVNPPSSTHMPCGPQVTPSVLRGQPDPQLEQLAGLLRLALPQTPTDQQLQQLLGSLISSVSLRPALLPVPPREDAAVDHNPTGQGQRSTSGDEMGHSLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LLA1</accession>
    <name>A0AAR2LLA1_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ABCB7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LLA1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000043229.1">
        <property type="protein sequence ID" value="ENSPNAP00000077330.1"/>
        <property type="gene ID" value="ENSPNAG00000036670.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000285477"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47239">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47239:SF1">
        <property type="entry name" value="EXTENSIN-3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="522" mass="54190" checksum="FA83AA007A24DFFB" modified="2024-11-27" version="1">SSSSSSSSSSSSKDPRNPEKTVATSRNATVNARLSATSRNATVNARLSVTSRNATVNAHLSATSRNATVNARLSATVTSRNATVNARLSVTSRNATVNARLSATSRNATVNARLSATATSRNATVNARLSATSRNATVNARLSATSRNATVNARLSVTSRNATVNARLSATSRNATVNARLSATSRNATVNARLSATSRNATVNARLSVTSRNATVNARLSATSRNATVNARLSATSRNATVNARLSATSRNATVNARLSATSRNVTVNARLSAMSRNATVNARLSVTSRNATVNARLSVTSRNATVNARLSATSRNATVNARLSATSRNATVNARLSATSRNVTVNARLSATSRNATVNARLSATSRNATVNARLSATSTNATVNARLSVTSRNATVNARLSATSRNATVNARLSATSRNATVNARLSVTSRNATVNARLSASSRNATVNARLSATSRNATVNARLSASSRNATVNARLSVTSRNATVNARLSATSRNATVNARLSVTSLHSCDLLPWKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LN60</accession>
    <name>A0AAR2LN60_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TBX22</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LN60"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070658.1">
        <property type="protein sequence ID" value="ENSPNAP00000077988.1"/>
        <property type="gene ID" value="ENSPNAG00000001458.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161206"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000001">
        <property type="entry name" value="T-box transcription factor TBX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF116">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="94"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="504" mass="55521" checksum="97B529768749BD0D" modified="2024-11-27" version="1">MQGLSSRAHAFSVEALVGKSCKRLKVETVKEEKLQTIPITDRDPDTGGEEGEGERDCSYSGDVRPNRETARDGAGPGTTTEEPCDPEPQVRVDLQGSDLWRRFHEIGTEMIITKAGRRMFPSIRVKVRNLDPTEQYHIAMDIMPVDSKRYRYVYHSSQWMVAGNTDHSCITPRLYVHPDSPSSGETWMRQVISFDRVKLTNNEMDDKGHIILQSMHKYKPRVHVIVREPGPEPVLLTSLPARGVHTFTFPETQFTTVTAYQNQQITKLKIDRNPFAKGFRDPGRNRGVLDGILESYPWRSPFGLDLKPFSLELQGGSSGSSGSCGTISPLKSLLPHSSSPASHPFTLSSPDSPLHGLSVPLCYKVTPPNHPFASRAYCSLGPDRLRGFPALRPLTDFPLLTPVHGKKAGSCRGQCLQAPTAIGPCLLSIQNLQASRSSGAHQAQQNALASSLAPPYGLYSYSLPLSPHFSTITNPLKAADSVTLSPSENILHQTAWDSPINHCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LT93</accession>
    <name>A0AAR2LT93_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CAMK4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LT93"/>
    <dbReference type="Ensembl" id="ENSPNAT00000074054.1">
        <property type="protein sequence ID" value="ENSPNAP00000077531.1"/>
        <property type="gene ID" value="ENSPNAG00000007564.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160006"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14085">
        <property type="entry name" value="STKc_CaMKIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000279">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="44"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000077531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="390" mass="43552" checksum="EDB8A3238487E691" modified="2024-11-27" version="1">VKESFVISCILSQCNIITDVKMGVACSPEYWIDGSKKETLADFYELESELGRGATSVVFRCRQKGTQKPYAVKMLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPSEISLVLELVTGGELFDRVVEKGYYSERDAADAIKQVLEAVAYLHENGVVHRDLKPENLLYATSAPDAPLKIADFGLSKIMDDQATMKTVCGTPGYCGELVNTAPFTVGHLQNFLFWFCLSPPAPEILRGCAYGPEVDMWSVGVITYILLCGFEPFFDDRGDQYMFKRILNCEYEFVSPWWDNVSLNAKDLVKKLIVQDPKKRLTTLQALQHPWVTGKAANFTHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSAGGHGNTDGNKTNCNHSSMQQGELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LVK3</accession>
    <name>A0AAR2LVK3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TEAD4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LVK3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057613.1">
        <property type="protein sequence ID" value="ENSPNAP00000078769.1"/>
        <property type="gene ID" value="ENSPNAG00000024468.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182956"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000001">
        <property type="entry name" value="Transcriptional enhancer factor TEF-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016361">
        <property type="entry name" value="TEF_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002603">
        <property type="entry name" value="TEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00554">
        <property type="entry name" value="TEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="8">
        <location>
            <begin position="30"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="7">
        <location>
            <begin position="30"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000078769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="427" mass="48173" checksum="B7E5879CAE9D6280" modified="2024-11-27" version="1">MRGVAPCWVSAPTPGGASEELNEALDKPLENDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKAREIQVKLKDQAAKDKALQNMATMSSAQIISATAFQNKMALQGLSRPAYPAAGFWHGALPGQPAPTEDIKPFSQQNYVMQASGPPITGYESASGLSMSPSAPPWQGRSIASSKLRMLEFSAFLEQPQDPENFNKHLFVHIGQSNPTYSDPYLEAVDIRQIYDKFPEKKGGLKELFEKGPPNAFFLVKFWADLSMNLQDDSSFFYGVSSQYESSDNMIITSSTKVCSFGKQVVEKVETEYARFENGRYVFRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTLETLLCVAYVFEVSTSEHGAQHHIYRLVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M1L6</accession>
    <name>A0AAR2M1L6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thymidine phosphorylase</fullName>
            <shortName evidence="5">TP</shortName>
            <ecNumber evidence="5">2.4.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">TdRPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TYMP</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>thymidine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + thymine</text>
            <dbReference type="Rhea" id="RHEA:16037"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:17821"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; dTMP biosynthesis via salvage pathway; dTMP from thymine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.4" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2M1L6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000055164.1">
        <property type="protein sequence ID" value="ENSPNAP00000080807.1"/>
        <property type="gene ID" value="ENSPNAG00000010241.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009250"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009032">
        <property type="term" value="F:thymidine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006213">
        <property type="term" value="P:pyrimidine nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000003">
        <property type="entry name" value="Pyrimidine-nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="entry name" value="Pyrimidine nucleoside phosphorylase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018090">
        <property type="entry name" value="Pyrmidine_PPas_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004490">
        <property type="entry name" value="PRK05820.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02644">
        <property type="entry name" value="Y_phosphoryl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="THYMIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515:SF0">
        <property type="entry name" value="THYMIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000478">
        <property type="entry name" value="TP_PyNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="Pyrimidine nucleoside phosphorylase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyrimidine nucleoside phosphorylase C-terminal" evidence="7">
        <location>
            <begin position="364"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000080807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="473" mass="51135" checksum="984E5D11CB94E4DF" modified="2024-11-27" version="1">TSNHPVMDHAISFQEMIRRKRDGGQLSSEEICAFIQGVKLANIQECQIGAMLMAIWQKGMVNEETLALTKEMMMSGEVFHWPEEWAELMVDKHSTGGVGDKISLPLAPALAACGCKVPMISGHGLAHTGGTLDKLESIPGFNIHQSVEQVRQILQDVGCCIVGQTETLVPADRVLYALRDATATVDSLPLITGSIISKKGAEGLYALLLDVKFGRAALYKDLNSARTLAQSLVNAGNSLGTRTGAVLSRMDTPIGRCVGNALEVCEALECLKGRGPDDLKELVTFLGGCLLWMSHRSPSLENGQRMIAEVLTNGAALHKFQAMLEAQGVSTQIAHSLCSQTTDYFKHLRRAEYQTELQASDDGTVVDIDGMVIATVLHKLGAGRTRAGQSINHSVGVELLADLGQQIQRGQSWIRIHHDCPVLSPEQHSDLQGSLLIGGRDSYQTRARVAEFIQPPDTRLTPQDPSEPDGSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2M322</accession>
    <name>A0AAR2M322_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Magnesium dependent phosphatase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2M322"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050094.1">
        <property type="protein sequence ID" value="ENSPNAP00000081444.1"/>
        <property type="gene ID" value="ENSPNAG00000025067.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165797"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="217" mass="23278" checksum="3CEFE3D836817A55" modified="2025-10-08" version="2">MQPLSLSLSLSPSLSLCLSLPLSHTHSLCLSLTLSPSLSLSLALSLSPLTLSLSLSLSPSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSGQVRDSSLCPLHLFDDTVEILRSLQTQGIPIGIASRTGDTDGANQLLSLFDLEQYVSFREIYPGSKVAHFSRLQAASGVPYTDMIFFDDEERNITDVSRLGVHCVLVDDGVTSSLVNKELQQFLMKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-07-06" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A158V3X4</accession>
    <name>A0A158V3X4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cocaine- and amphetamine-regulated transcript protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2014-02" db="EMBL/GenBank/DDBJ databases">
            <title>Appetite regulators in Characiforme fish.</title>
            <authorList>
                <person name="Volkoff H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Brain</tissue>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CART family.</text>
    </comment>
    <dbReference type="EMBL" id="KJ415178">
        <property type="protein sequence ID" value="AII30207.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017545137.1">
        <property type="nucleotide sequence ID" value="XM_017689648.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A158V3X4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000033232"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023909.2">
        <property type="protein sequence ID" value="ENSPNAP00000033232.1"/>
        <property type="gene ID" value="ENSPNAG00000021749.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108416756"/>
    <dbReference type="CTD" id="550232"/>
    <dbReference type="GeneTree" id="ENSGT00390000018319"/>
    <dbReference type="OMA" id="CNCPRGA"/>
    <dbReference type="OrthoDB" id="9936511at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008343">
        <property type="term" value="P:adult feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032099">
        <property type="term" value="P:negative regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22741">
        <property type="entry name" value="CART_CTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.30:FF:000001">
        <property type="entry name" value="Cocaine-and amphetamine-regulated transcript protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.30">
        <property type="entry name" value="CART, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009106">
        <property type="entry name" value="CART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036722">
        <property type="entry name" value="CART_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655">
        <property type="entry name" value="COCAINE AND AMPHETAMINE REGULATED TRANSCRIPT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655:SF5">
        <property type="entry name" value="COCAINE- AND AMPHETAMINE-REGULATED TRANSCRIPT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06373">
        <property type="entry name" value="CART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64546">
        <property type="entry name" value="Satiety factor CART (cocaine and amphetamine regulated transcript)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="6" id="KW-0529">Neurotransmitter</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044549322" description="Cocaine- and amphetamine-regulated transcript protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AII30207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000033232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="116" mass="13018" checksum="0C88EA056AE89CB0" modified="2016-07-06" version="1">MESSSVRGALLLLLLLSASAQETDTTDNELELDTRAIRDFYPKDPNLTSEKQLLGALHEVLEKLQTKRIPPWEKKFGQVPMCDVGEQCAVRKGSRIGKMCDCPRGAFCNFFLLKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNP4</accession>
    <name>A0A3B4BNP4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017542679.1">
        <property type="nucleotide sequence ID" value="XM_017687190.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNP4"/>
    <dbReference type="SMR" id="A0A3B4BNP4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000049"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014519.2">
        <property type="protein sequence ID" value="ENSPNAP00000000049.1"/>
        <property type="gene ID" value="ENSPNAG00000004943.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414339"/>
    <dbReference type="CTD" id="7322"/>
    <dbReference type="GeneTree" id="ENSGT00940000153169"/>
    <dbReference type="OMA" id="VHFTTRI"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000101">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="147" mass="16744" checksum="240799E1CDC9FACE" modified="2018-12-05" version="1">MALKRIHKELHDLGRDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNV8</accession>
    <name>A0A3B4BNV8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AMOP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isthmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540106.1">
        <property type="nucleotide sequence ID" value="XM_017684617.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNV8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001437"/>
    <dbReference type="Ensembl" id="ENSPNAT00000012157.2">
        <property type="protein sequence ID" value="ENSPNAP00000001437.1"/>
        <property type="gene ID" value="ENSPNAG00000008163.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412548"/>
    <dbReference type="CTD" id="553163"/>
    <dbReference type="GeneTree" id="ENSGT00940000163359"/>
    <dbReference type="OMA" id="HKAHGHP"/>
    <dbReference type="OrthoDB" id="9930623at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051867">
        <property type="entry name" value="Angio_Inhib/Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239">
        <property type="entry name" value="ISTHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10239:SF31">
        <property type="entry name" value="ISTHMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017357028" description="AMOP domain-containing protein" evidence="7">
        <location>
            <begin position="26"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="8">
        <location>
            <begin position="313"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="488" mass="54820" checksum="7785F2792D7AE0F1" modified="2018-12-05" version="1">MFGASKNVCVPLSVAFVVSLELVLCFPTRQNKSATARFRHGAAAEAHIQTSSSGISEGHDLEQNQVQSVLTGSYQLQQQQYHRWSQQHSLGVLPQPEPEEESKPFILDLKNFPDLASADTGSQNPNIQVTIEVVDDPQMEVEMDLVKERNSDWPISSSSPSPVVDWLGGKKLFWPLFWGYTDVDSGEDSTDQGVKEVVEDEKFEEEEEENYTSDFDSQEPLPSGLGGIDGELESQWNLGWDFTHRNTEKDEDNWSAWGPCSVTCGHGNQTRTRSCGDFCMSTESRSCYLNPCPDDLNPISSVFPFEMENGTEPFGTDIDSCEKWLNCKSEFLQRYLQQVLMELPNCPCSYPTTASHSALNLPDEGHKQTFHWRDASNPKERLDIYKPSARSCLRSALSAQGTTLAAQHCCYDSQGHLITRGKGAGTPNLISTEFSPELHFKVDILPWILCKGDWSRFHAVRPPNNGLNCPENPRQDVFMNELEEAREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BNY5</accession>
    <name>A0A3B4BNY5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BNY5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000149"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014345.2">
        <property type="protein sequence ID" value="ENSPNAP00000000149.2"/>
        <property type="gene ID" value="ENSPNAG00000007302.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230358"/>
    <dbReference type="OMA" id="SHTVMCK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043814535" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000149.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="120" mass="13351" checksum="E563395E15692864" modified="2024-11-27" version="2">MKITLSLLLILLSFSSVKCESMESLETGVQLKPGETLSLSCRGSGFTFSSYDMNWIIQQAGKPLEWIGYISNLAYSTNYAKSYQGRAEITRDNSKSMAYLKLSGVTVEDSAVYYCARDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BP54</accession>
    <name>A0A3B4BP54_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BP54"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000000261"/>
    <dbReference type="Ensembl" id="ENSPNAT00000014167.2">
        <property type="protein sequence ID" value="ENSPNAP00000000261.2"/>
        <property type="gene ID" value="ENSPNAG00000007391.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163342"/>
    <dbReference type="OMA" id="ITDNDDC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="40"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000000261.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="298" mass="34866" checksum="16A2EB8D20A31EDB" modified="2024-11-27" version="2">DPIHDFSWFSRPEVFDFEGISMIHYFTDNWENIQNFKARPDDILIATYPKAGTTWVSYVLDLLYFSKLGPERQNSLPIFLRVPFLDGAFPGLPTGVELADMLPTTPRLIKTHLPVQLVPKSFWEQNCRVIYTARNAKDNAVSYYHFNRMNMGQPDPGDWSTFLQTFMDGKTVFGPWYDHVCGYWEKKQTHSNIHYMFFEDMVEDTGREVERLCSFLGSSTSAEEKERITKATQFDAMKQNKMTNYSTVPVMDSKISPFMRKGKVGDWKNHFTVAQNEQFDEHYKQKMKSTTLQFRTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BST5</accession>
    <name>A0A3B4BST5_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TIMELESS</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560256.2">
        <property type="nucleotide sequence ID" value="XM_017704767.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017560258.1">
        <property type="nucleotide sequence ID" value="XM_017704769.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017560259.1">
        <property type="nucleotide sequence ID" value="XM_017704770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BST5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001615"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011918.2">
        <property type="protein sequence ID" value="ENSPNAP00000001581.1"/>
        <property type="gene ID" value="ENSPNAG00000008298.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431541"/>
    <dbReference type="CTD" id="8914"/>
    <dbReference type="GeneTree" id="ENSGT00390000015124"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007725">
        <property type="entry name" value="TIMELESS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26019">
        <property type="entry name" value="HTH_TIMELESS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05029">
        <property type="entry name" value="TIMELESS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="527"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="941"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1110"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="527"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="972"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1114"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1163"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1217"/>
            <end position="1230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1250" mass="144333" checksum="4D683384ED207BBD" modified="2018-12-05" version="1">MDLYMMNCELLATCSALGYLEGETYHKEPDCLESVKDLIRYLRHEDDSRDIRQQLGAAQILQNDLLPIITQHGQDKPLFDACIRLMVNLTQPALLCFGKIPDDPAFRHHFLQVVSYLQAYKEAFANEKVFTVLSETLYNLLQLDWEQRQEEDNLLIERILLLVRNVLHVPADPYEEKNVDDDASVHDKLLWAIHMSGMDDLLKFLASAQSEQQWSMHVLEVISLMFREQTPELLVRAGQVRSAQEKQKDSNELEALRQKELAEKHSRTMQRGTRHSRFRGSYVVQGLKSISEKDVIFHQGLHNFKNYSHDAGKATRRVPKRKQVAQDTKSQRRSVLNVRLFLREFCADFLDNCYNRLMYLVKESLIREHAQQHDETYYLWALSFFMAFNRGNGCKAELVSETLSVRTFHFIEKNITNYYEMMLTDKKEATSWSRRMHLALKAYQELLLTVNEMDRSKDENIRQSASVIKSNIFYLMEYREIFLTLLRKFDEKMQPRSFLQDLVESTHLFLRMLERFCKGRSNLLVQKKKVKRKKQKKKSSSHTENTPEVLEETWTIVSQELRTSGFELSNALTEGVVPFDAASEIPVAEQRTEAMIRIQDALFARTGPEALGLLRAAREVWPEGDVFGSAEVQPEEELELLKQILFAELPRPAHTEATMDEDEEVEELEEEELESVRVSETEFNFLDFIKRFANPSIIRPYLLLLRSYASNTPHTNHCIVRMLHRVAVDLKMDALLFQLSVFYLFNKVLTDPAAAVYKELVTFAKYILNRFFALATRNNKAFIELLFWKNVGAVREMTEGYRKEGEDGMKKVKWTPEEEEELRQLYEEHKDSGVPDIVETLLPLLMNTNRTRRQVVTQMVSMGLVDSVKDLKKEKKGTCIVLWTEDQEQELQMLFEEYKDSDDVLGNILKRLTAKRSRARVVDKILSLGLVSERRDLYKKRRRNAPGKSTGMSEEDFFASVSGAPEENTMDRDEEEDDDLEEDKDDSEEERDVVRPAQGKKGRRRESAVERNVNVGGVVQDLRREGLSGPVLWLQNCLNRAADDREEDGVSHPMPLVPLTEENEDAMENKSFKRLLHKIGIRPPADEQESFWRIPATMSIYQLRTMASSLNPPEESEVGGEGESESGVAVNLEAEQQDEGSDGQELRAQAIRALLLSRQRKRSSADKKKDPSVEEESPESEAQSAGAGIDRKTSVKRNRVLDSDDERDDDSPPKMNVDTEHQDGSGEEAHSAPAKRRRQRVLSDDEDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BSW0</accession>
    <name>A0A3B4BSW0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cilia and flagella associated protein 73</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BSW0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000001605"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011876.2">
        <property type="protein sequence ID" value="ENSPNAP00000001605.2"/>
        <property type="gene ID" value="ENSPNAG00000008265.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193995"/>
    <dbReference type="OMA" id="SIEMLYI"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051147">
        <property type="entry name" value="CFAP_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025252">
        <property type="entry name" value="DUF4200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21683">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 42 LIKE-2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13863">
        <property type="entry name" value="DUF4200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4200" evidence="3">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000001605.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="277" mass="32012" checksum="89EBA78EB0633630" modified="2024-11-27" version="2">TFLTNCCLKCVYVCLCVPLQEFEVKKESLRQRREELKIKEEKLKDSLLKFDKYLKENDAKKTRGLKKAEAERAVVREREREERQLQRNIAALLAKKEQLQGRVNRNRVYCSFLDDVLKASKKFEDVGQLIGRFDALVCTREQLLKRQSEVESERETEGVELRRYVSERGSALLHYNNGLSQLQTELDTILSQALRWESAWNHIQATAAKETLLLGQIKVVTLNLYHLTGVVAGGAEGVDVDDTLEQLDKIQLYIQDRADIVRDLRSDTDNRSTSDHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BUS1</accession>
    <name>A0A3B4BUS1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cyclosome subunit 16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ANAPC16</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the APC16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017559044.1">
        <property type="nucleotide sequence ID" value="XM_017703555.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BUS1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002255"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010750.2">
        <property type="protein sequence ID" value="ENSPNAP00000002255.1"/>
        <property type="gene ID" value="ENSPNAG00000008680.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430819"/>
    <dbReference type="CTD" id="119504"/>
    <dbReference type="GeneTree" id="ENSGT00390000018109"/>
    <dbReference type="OMA" id="GASRRCH"/>
    <dbReference type="OrthoDB" id="6374621at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029641">
        <property type="entry name" value="APC16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31564">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31564:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17256">
        <property type="entry name" value="ANAPC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="108" mass="11637" checksum="83CD55EF32FEB5F2" modified="2018-12-05" version="1">MAASSSSSSAGGVSGSSVTGSGFSASELIPPRKVLYTYPKGAGEMMEDGSDRFLCESVFSYQVASTLKQVKHDQQVSRMEKLASLVEELEADEWKYKPIEQLLGFTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BVS7</accession>
    <name>A0A3B4BVS7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clustered mitochondria protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria. Specifically binds mRNAs of nuclear-encoded mitochondrial proteins in the cytoplasm and regulates transport or translation of these transcripts close to mitochondria, playing a role in mitochondrial biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CLU family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550377.1">
        <property type="nucleotide sequence ID" value="XM_017694888.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000002605"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010149.2">
        <property type="protein sequence ID" value="ENSPNAP00000002605.2"/>
        <property type="gene ID" value="ENSPNAG00000009024.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425870"/>
    <dbReference type="CTD" id="562666"/>
    <dbReference type="GeneTree" id="ENSGT00390000012485"/>
    <dbReference type="OMA" id="HPVWDKD"/>
    <dbReference type="OrthoDB" id="1414216at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000088">
        <property type="entry name" value="Clustered mitochondria (CluA/CLU1) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2280.10:FF:000001">
        <property type="entry name" value="Clustered mitochondria (CluA/CLU1) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03013">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028275">
        <property type="entry name" value="CLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF10">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="6">
        <location>
            <begin position="381"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="685"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1390"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="744"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="766"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1396"/>
            <end position="1417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002605.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1417" mass="157517" checksum="6499BBFFC1D331F9" modified="2024-11-27" version="2">MVSKTDDIPASVPNCTAVDLAEDGETPDSAESDRTALKKPCPCEHSASAVVMNGDAGHDQAEEAESKQDSSGDADPGDDGNEQEVIVIQDTGFTVKIQAPGTEPFDLQVSPQEMVQEIHQVLMDREDTCHRTCFSLQLDGNVLDNFAELKSIEGLQEGSLLKVVEEPYTVREARIHVRHIRDLLKSLDPSDAYNGVDCNSLSFLSVFTDGDLGDSGKRKKKGSELEQIDCTPPEHILPGSKERPLVPLQPQNKDWKPLQCLKVLTMSGWNPPPGNRKMHGDLMYLSVVTVEERNVSITASTRGFYLNQSTTYTFNPKPANPSFLSHSLVELLSQISPAFKKNFTALQKKRVQRHPFERIATPFQVYSWTAPQIDHAMDCVRAEDAYTSRLGYEEHIPGQTRDWNEELQTTRELPRKNLPERLLRERAIFKVHSDFAAAATRGAMAVIDGNVMAINPGEETRMQMFIWNNIFFSLGFDVRDHYKELGGDAAAHAAPTNDLNGVRAYGAVDVEGLYTLGTVVVDYRGYRVTAQSIIPGILEREQEQSVIYGSIDFGKTVVSHPKYLELLEKTSRPLKVQRHCVLNEKDTAVELCSSVECKGIIGNDGRHYILDLLRTFPPDLNFLPVEGEELSPESQKQGFPRQHRHRLACLRQELIEAFVEHRYLLFMKMAALQLMQQKANKESKTAALPESISPESVSDSKPTAAPEASEKASEAATPAFDSPTSSESTLTPEDTAESEAALGQETTSESQETSARDSSAVPPVTANGTHEPTIPESQNGGCDSPLEGKEADEAIPELAQAKELAESLAADDGSGIDPKSREVVLNACKAVGSISNTSFDIRFNPDIFSPGVRFPEDSAEEIQKQKQLLKDAAAFLVSCQIPSLIKDCLDHTALPMDGATLTEALHQRGINVRYLGNVLEFVDKMPAKPQLEHVYRIGITELITRCTKHIFKTYLQGVELSALSAAVSHFLNCFLSSFPDAVAHLPADELVSRKKNKRRRNRVPGGGDNTAWASLTPSELWKNIASEALSYYHFTLQCESADQAVEKYGLQKITLLREISVKTGIQILIKEYNFDSRHKPAFTEEDILNIFPVVKHVNPKASDAFHFFQSGQAKVQQGFLKEGCELINEALNLFNNVYGAMHVEICACLRLLARLNYIIGDHPEALSNQQKAVLMSERVLGIEHPNTIQEYMHLALYCFANGQLSTALKLLYRARYLMLLVCGEDHPEMALLDSNIGLVLHGVMEYDLSLRFLENALSINSKYHGPRSLKVALSNHLVARVYESKAEFRSALQHEKEGYTIYKNQVGEAHEKTKESSEYLKYLTQQAVALQRTMNEIYKNGSNASIMPLKFTAPSMASVLEQLNIINGIIFIPLSQKDLENLKAEVQRRQQLQDSGKAEEHQEDSQLVLDDKLPVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZG5</accession>
    <name>A0A3B4BZG5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 16">Amyloid-beta A4 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">APP</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G(O).</text>
    </comment>
    <comment type="function">
        <text evidence="16">Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 16">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZG5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003874"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007961.2">
        <property type="protein sequence ID" value="ENSPNAP00000003874.1"/>
        <property type="gene ID" value="ENSPNAG00000010344.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429850"/>
    <dbReference type="CTD" id="58083"/>
    <dbReference type="GeneTree" id="ENSGT00530000063252"/>
    <dbReference type="OMA" id="THRVQKC"/>
    <dbReference type="OrthoDB" id="6147836at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005798">
        <property type="term" value="C:Golgi-associated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030546">
        <property type="term" value="F:signaling receptor activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22607">
        <property type="entry name" value="Kunitz_ABPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.140:FF:000001">
        <property type="entry name" value="Amyloid beta (A4) protein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013803">
        <property type="entry name" value="Amyloid_glyco_Abeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF7">
        <property type="entry name" value="AMYLOID-BETA PRECURSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03494">
        <property type="entry name" value="Beta-APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00203">
        <property type="entry name" value="AMYLOIDA4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00204">
        <property type="entry name" value="BETAAMYLOID"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0034">Amyloid</keyword>
    <keyword evidence="16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0186">Copper</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017354395" description="Amyloid-beta A4 protein" evidence="19">
        <location>
            <begin position="19"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="695"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="21">
        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="20">
        <location>
            <begin position="308"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="22">
        <location>
            <begin position="372"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="15">
        <location>
            <begin position="29"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="15">
        <location>
            <begin position="132"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="193"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="625"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="397"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="238"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="74"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="134"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="764" mass="86199" checksum="5F03D8C02DEEBE67" modified="2018-12-05" version="1">MGDRGVFLLLMAVASAMALEVPSDEGAGLLAEPQVAMFCGKLNMHINVQNGKWELDPSGTKSCIGTKEGILQYCQEVYPELQITNVVEANQPVSIQNWCKKGRKQCRSHMHIVVPYRCLVGEFVSDALLVPDKCKFLHQERMDICESHLHWHTVAKESCGDHTMNLHDYGMLLPCGIDRFRGVEFVCCPAEAEREADSDDMEEDDSDVWWGGAEADYSDTSMAREAGPAEQAEAEPVAAEDDEDVEDQEEETLDNDQDGDGDEEEEEDEEDDEMIDERSDNEQTTSVAMTTTTTTTTESVEEVVREVCWANAETGPCRAMLPRWYFVKEEGHCAPFIYGGCGGNRNNFESEEYCLSVCSSVLPTTSSSSPDAVDHYLETPADENEHAHFQKAKESLEAKHRERMSQVMREWEEAERQSKNLPRADKKAVIQHFQEKVEALEQEAASERQQLVETHMARVEALLNDRRRIALESFLTALQADQPRPRHVFSLLKKYVRAEQKDRQHTLKHFEHVRVVDPKKAAQIRPQVLTHLRVIEERMNQSLGLLYKVPGVVEDIQDQVELLQREQQEMSAQLASLQSDVRVSYGNDALMPDLPDSTASLDMLPSEDSQRLGLDGLGFIHPESFNQPNTQNHVEPVDARPIPDRGLPTRPVSGLKPEDVPELKMEAEERHSAGYEVHHQKLVFFAEDVSSNKGAIIGLMVGGVVIATVIVITLVMLRKKQYTSIHHGVIEVDAAITPEERHLSKMQQNGYENPTYKFFEQMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BZU1</accession>
    <name>A0A3B4BZU1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Palmitoyltransferase</fullName>
            <ecNumber evidence="12">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CXCR5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4BZU1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004145"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007511.2">
        <property type="protein sequence ID" value="ENSPNAP00000004145.2"/>
        <property type="gene ID" value="ENSPNAG00000010586.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156558"/>
    <dbReference type="OMA" id="SLYGISM"/>
    <dbReference type="OrthoDB" id="430659at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF475">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="183"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004145.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="366" mass="41245" checksum="F15DB6E636A82AD4" modified="2024-11-27" version="2">MNIIELIQRDSKNEDFLSRLLSVLSERLGLQCSPLGSVRIELSILPPMLLLPVLLQVAALHCVLGVVVLTALPGLVLWYYYMTHRKKRRTLFFLSLALFSLAYMYYLFITEIVPRGDVSYQQVVTATTGMILMLISLVRTKQGPGYVQADNRHNNITSTNQSKLCNQDSACCNGVSQSDHAVENKRKWCSICRLVRPSRAGHCRICGVCVLRMDHHCVWINCCVGQDNHRHFLLTLLLFLLTSLYGISMVLRSVCPRQSVFTALLYCPGVYNQYSTALCFTCVWYSCIITGGLLHLLILQIINISCNVTEREVQLALRNGTGRRRFCGLLVETSTPSHGFMHNWTHFLTMHTAEGAQAPLTLTDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C0S3</accession>
    <name>A0A3B4C0S3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sulfotransferase</fullName>
            <ecNumber evidence="15">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HS3ST2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017562953.1">
        <property type="nucleotide sequence ID" value="XM_017707464.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C0S3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004269"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007288.2">
        <property type="protein sequence ID" value="ENSPNAP00000004269.1"/>
        <property type="gene ID" value="ENSPNAG00000010709.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433116"/>
    <dbReference type="CTD" id="9956"/>
    <dbReference type="GeneTree" id="ENSGT00940000160498"/>
    <dbReference type="OMA" id="FCRSTIM"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000194">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF10">
        <property type="entry name" value="HEPARAN SULFATE GLUCOSAMINE 3-O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="18">
        <location>
            <begin position="135"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="82"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="12">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="143"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="349"/>
            <end position="353"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="386" mass="43825" checksum="C7CB38FF215299BB" modified="2018-12-05" version="1">MAYRVLSSRIVPPSNKLSRRFLFVFTLSLSFTYLCYSVLFCSGTVMPRLHGFEEHGCARLESAGAKKLLQKAPFCAAESAQSGPNAALPRSEAASPARNQSGRRAAAQSARTEAARSKAGNGTSAQRYGNKKLPNALIVGVKKGGTRAVLEFIRIHPDVRAVGTEPHFFDRNYDKGLDWYRGLMPRTLDSQITMEKTPSYFVTREAPRRIASMSRETKLIVVVRNPVTRAISDYTQTLSKKPDIPTFEELAFKNRSQGVVDTSWNAIRIGMYILHLENWLQYFRLSQIHFVSGERLITDPAGELGRVQDFLGLKRIITDKHFYFNRTKGFPCLKKPESSSLPRCLGKSKGRTHVQIDQEVIEQLRDFYRPFNVKFYEMVGHDFRWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5W8</accession>
    <name>A0A3B4C5W8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5W8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007103"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002409.2">
        <property type="protein sequence ID" value="ENSPNAP00000007103.2"/>
        <property type="gene ID" value="ENSPNAG00000012999.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="OMA" id="GSSNIGX"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="2">
        <location>
            <begin position="25"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="1">
        <location>
            <begin position="35"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007103.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13793" checksum="0AA56751C4ABF70F" modified="2024-11-27" version="2">LDYTDSLICYFLYLSSVSHQEVQSPEFLSVEAGKTVSISCTGTSGVGSDMSWYLLRPGEAPKLLIYGGDALESGVPERFSGSYSGLNFQLTISGVQPEDAGDYYFYYCQSQHNTGTSTNKVHVFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C814</accession>
    <name>A0A3B4C814_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cullin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C814"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006584"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003300.2">
        <property type="protein sequence ID" value="ENSPNAP00000006584.2"/>
        <property type="gene ID" value="ENSPNAG00000002328.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154774"/>
    <dbReference type="OMA" id="IREWDRY"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000161">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000007">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000011">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000019">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000003">
        <property type="entry name" value="Cullin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000023">
        <property type="entry name" value="cullin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1030.10:FF:000001">
        <property type="entry name" value="Putative Cullin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1030.10">
        <property type="entry name" value="Ring Box Chain A, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="11">
        <location>
            <begin position="426"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006584.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="785" mass="91035" checksum="42725F191ACCDC85" modified="2024-11-27" version="2">MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGPGVPPSKPSKKAPTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRECLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLSVYKEYFETQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRITDGLGELKKLLETHIYNQGLAAVEKCGEAALNDPKMYVQTILDVHKKYNALVMSAFNNDAGFVAALDKVFTMLLFQACGRFINNNAVTKMVQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYHLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDVYTVQQLTDSTQIKIDILVQVLQILLKSKLLVLEDENANVDEVEFKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKMLKHQQLLAEVLNQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C844</accession>
    <name>A0A3B4C844_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">palmitoyl-protein hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017580840.1">
        <property type="nucleotide sequence ID" value="XM_017725351.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C844"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000737.2">
        <property type="protein sequence ID" value="ENSPNAP00000008082.2"/>
        <property type="gene ID" value="ENSPNAG00000032238.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108444271"/>
    <dbReference type="CTD" id="793871"/>
    <dbReference type="GeneTree" id="ENSGT00730000111100"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070646">
        <property type="term" value="P:protein modification by small protein removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF7">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="6">
        <location>
            <begin position="8"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008082.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="169" mass="18615" checksum="1FA87DA209AE2694" modified="2024-11-27" version="2">MDNQASTYSVKLYIYDLSKGLARQLSPMMLGKQLEGIWHTAIVVHGSEFFYGVQGIASCPPGGTLLGEPNAIVDLGNTEVNLDLFTEYLSSLGESTYRADSYQLFEHNCNSFSNEVAQFLTGRKIPSYITDLPSEVLSTPFGQALRPLIDSINIYPPGNNAMNGYYGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8L5</accession>
    <name>A0A3B4C8L5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="11">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8L5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002145.2">
        <property type="protein sequence ID" value="ENSPNAP00000007251.2"/>
        <property type="gene ID" value="ENSPNAG00000013175.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183148"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052576">
        <property type="term" value="P:carbohydrate storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04300">
        <property type="entry name" value="GT35_Glycogen_Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000005">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000153">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000197">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF11">
        <property type="entry name" value="ALPHA-1,4 GLUCAN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007251.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="910" mass="104728" checksum="21BEF561C17341C0" modified="2024-11-27" version="2">MTLQLIHFKSKWQIVFLGAIPEEVRCNIIHLHKDGETERKATGNCHHHINTFLLLAPPLWTHTQISGTMSKPLSDHDKRKQISVRGLAGVENVTDLKTNFNRHLHFTLVKDRNVATRRDYYFALANTVRDHLVGRWIRTQQHYYEKDPKRVYYLSLEFYMGRTLQNTMVNLALENACDEAVYQLGLDMEELEDIEEDAGLGNGGLGRLAACFLDSMASLGLAAYGYGIRYEFGIFNQKIVNGWQVEEADDWLRYGNPWEKARPEYMRPVHFYGRTEHHPDGVKWVDTQVVLALPYDTPVPGYRNNVVNTMRLWSAKAPCEFNLKDFNVGGYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKVSKFGSREIARTDFNKLPEKVAIQLNDTHPALAIPELMRVLVDEEKLPWDKAWDITVRTCAYTNHTVLPEALERWPIDLFQNLLPRHLEIIYEINRRHLERVASLFPGDTDRMRRMSLIEEGGIKRVNMAHLCIVGAHAVNGVARIHSDILKATVFKDFYEMDPHKFQNKTNGITPRRWLVMCNPGLAEVIAERIGEDFIRDLDQLKKLLKFVNDEAFIRDVAKVKQENKLKFATHLEEHYKVKINPNSMFDIQVKRIHEYKRQLLNCLHIITFYNRIKKEPNKQWSPRTIMIGGKAAPGYHTAKMIIRLITAIGEVVNNDPVVGDRLKVIFLENYRVTLAEKAVPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEDNIFIFGMRVEDVEAMDKKGYNASEYYNRIPELKQAIDQIAGGFFSPKQPDLFKDIVNLLMHHDRFKVFADYEDYIKCQEKVSALYKKPKEWVKKVIYNIAGSGKFSSDRTISQYAREIWGVEPSLEKIAAPDDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8W1</accession>
    <name>A0A3B4C8W1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8W1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008362"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000263.2">
        <property type="protein sequence ID" value="ENSPNAP00000008362.1"/>
        <property type="gene ID" value="ENSPNAG00000013961.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165300"/>
    <dbReference type="OMA" id="VPAVHPN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021575">
        <property type="term" value="P:hindbrain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000029">
        <property type="entry name" value="Achaete-scute family bHLH transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF133">
        <property type="entry name" value="ACHAETE-SCUTE HOMOLOG 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="73"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="190" mass="21169" checksum="613C0F82C023B015" modified="2018-12-05" version="1">SLSSLSLSLSVSLFSLLSLSIFVSLSSLSLSPLSLLSLSVKRPPRSSSPELLRCKRRLTFSGLGYSLPQQQPVAVARRNERERNRVKQVNMGFQTLRQHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAISAAFRCGVPSPSLSHSYSAEPESPHSSYSSDDGSYEQLSSEEQELLDFTTWFDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CB30</accession>
    <name>A0A3B4CB30_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CB30"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000076.2">
        <property type="protein sequence ID" value="ENSPNAP00000008470.2"/>
        <property type="gene ID" value="ENSPNAG00000000146.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222294"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="148"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="241"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="327"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008470.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="414" mass="45546" checksum="A91017EC2067DF44" modified="2024-11-27" version="2">MKIWKHFDENVIMLKILQAAVMRSLRTAPPLPLVFLLMTAGAFGAEGSVKYNQQEICALKGSTVFMNGTFTHPEGLTVTKTFWVIDPIPGVEPTDLSKDPDYSSRVGFLTDEQKRFSLKLSDVMKKDEHWFCFRIITNEEKARWTGVPGVQLRVTELHVEAPEEVTEGQTADLTCKTTCSLTDPKFIWYKNGRPLTTKTIKNNQLHLQTVSSEDTGSYSCAVGGSQHLSSTAHSLRVRYPPKRVSVSISPSGEIVEGSSVTLTCRSDGNPPVKIFSWFKGSTSVGEGKTYSIPNIRSEDSGEYTCQSQNDHGERRSTAVQINVLYPPKRVSVSISPSGEIVEGSSVTLTCSSDGNPPVKNYTWYKEGGTSPVGSGHSYSIINITADHTGLYYCEAQNDHGALNGTVMVTVKSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CB32</accession>
    <name>A0A3B4CB32_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Platelet-derived growth factor (PDGF) family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">VEGFD</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017550149.1">
        <property type="nucleotide sequence ID" value="XM_017694660.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CB32"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000007639"/>
    <dbReference type="Ensembl" id="ENSPNAT00000001500.2">
        <property type="protein sequence ID" value="ENSPNAP00000007639.1"/>
        <property type="gene ID" value="ENSPNAG00000013389.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108425747"/>
    <dbReference type="CTD" id="2277"/>
    <dbReference type="GeneTree" id="ENSGT00940000159726"/>
    <dbReference type="OMA" id="IDMLWDN"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005172">
        <property type="term" value="F:vascular endothelial growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008015">
        <property type="term" value="P:blood circulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050930">
        <property type="term" value="P:induction of positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001946">
        <property type="term" value="P:lymphangiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060754">
        <property type="term" value="P:positive regulation of mast cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038084">
        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050507">
        <property type="entry name" value="PDGF/VEGF_growth_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025:SF11">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 3" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017241694" description="Platelet-derived growth factor (PDGF) family profile domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="5">
        <location>
            <begin position="99"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000007639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="294" mass="33497" checksum="AE9BB47E1B3DDEF5" modified="2018-12-05" version="1">MRTLLSIGLHTLLLLYVRLMLAKDTLASVRINQQKWERDIRSAGSLDELLMLTDFPDWKLWRCRLKLKHIDSTSLPEFRSASAGSHRSTRFADNSYSLEILKAIDEEWQKIQCMPRETCVDVAKELGTTTAMFFKPPCVSVYRCNGCCNKEGVTCRNTSTTYVHKTLLSVIPFEYGPEPILVKVANHTECKCQEPQLIRRHAHPHSSGCSPIRQLGDSRRLCDRGLIWDCMEGRCVPYPSSNDEISPTTKAEDCEIDVERCECIPKETTPKQQSPLCPSNYTMCAAKSRQFNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CD84</accession>
    <name>A0A3B4CD84_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leiomodin-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for proper contractility of visceral smooth muscle cells. Mediates nucleation of actin filaments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017540676.1">
        <property type="nucleotide sequence ID" value="XM_017685187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CD84"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009493"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035336.2">
        <property type="protein sequence ID" value="ENSPNAP00000009493.1"/>
        <property type="gene ID" value="ENSPNAG00000014980.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412940"/>
    <dbReference type="CTD" id="795335"/>
    <dbReference type="GeneTree" id="ENSGT00940000159825"/>
    <dbReference type="OMA" id="ELKMDMM"/>
    <dbReference type="OrthoDB" id="2163268at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000083">
        <property type="entry name" value="Leiomodin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF5">
        <property type="entry name" value="LEIOMODIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WH2" evidence="10">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="552"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="163"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="592"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="616"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="690" mass="78774" checksum="EF51B6D4C07551EA" modified="2018-12-05" version="1">MSRRKVRGLTRTGRQVSEDPDLDNLLSNLSPEEMEELQKEVSTVPDPDPSEIIVVDQTDLKRSTPHKKESSSSNREDESRMCLQRNLSTEGEPRRESRKQEYLRKMGLSQEKNVPKTDTRDVGLRRQASISTPIAPHERESKMENKTTKTSEELKNVRARHFRREEENAKNVEVTEKEQNDDCKFKERRETKDSSRKTKELISKLQDKKEDPKEKDRKEESRKRESVDSKTRDLISRLQEKQEKEERKERERRDEVRKKEDSKTKSLVSKLEEKQNQVVEIKTDEKKTEEREKTDKRPELVKSNDKNRDAIKNNVRKDTDSTDKENEKEKMDKAIVKGKEELKMSSVDKGKEEAKQQSDFQNINESSKEEKVGNCVAEKNTKTKSKEEEEEEESVSMFAEDLERVQCNNPGMTELNVNNSEVIKVKTLIQFAKALSNNTHIKTFALANCRADDHVAYAIAETLRNNKTITSINLDSNLLTSKGIMALVQALQHNSTLTELRFHNQRHICGGKTEMEMTKILKENTTLLKLGYHFELAGPRMTMTNILSRNMDRQRQKRLQEQKQAQAQGNGEKKDTLEVPKPGFTKGSPRSSPKPSPTPSPVPSPKLMPKKGSGGPLPPPPPPGGLPPPPPPIMDGDFLKNSLTPVSRRKLDDRTGGRGGSLNSRDQLLASIRGANIKQLKKVALPKLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CDY4</accession>
    <name>A0A3B4CDY4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CDY4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010152"/>
    <dbReference type="Ensembl" id="ENSPNAT00000035594.2">
        <property type="protein sequence ID" value="ENSPNAP00000010152.2"/>
        <property type="gene ID" value="ENSPNAG00000030512.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182932"/>
    <dbReference type="OMA" id="THEHIWP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050769">
        <property type="entry name" value="NAT_camello-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947:SF58">
        <property type="entry name" value="8B (PUTATIVE,_PSEUDO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947">
        <property type="entry name" value="GNAT FAMILY N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="60"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010152.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="216" mass="24280" checksum="896219A12E1D6BDC" modified="2025-10-08" version="3">MAAFRESEIPSDDEAVMAIFQNGTQEHIIPSFKYAISQPLHITVTLCFFTVGYILGGGSVVLALLAGGLWIGLLYYCCYEFYAGYVRSKLNTDMRDIAGFYLSSPDNCFWVAEAEINGQPQIMGIVAVEGKELPDSGQKYGELFRMNVLSTSRRTGLASRLCETVIDFCKERRFSKIVLHVTFTQTAAIAFYRKMGFALVHDLLFKVNNTMFNPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEY8</accession>
    <name>A0A3B4CEY8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEY8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015824.2">
        <property type="protein sequence ID" value="ENSPNAP00000009436.2"/>
        <property type="gene ID" value="ENSPNAG00000014922.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272237"/>
    <dbReference type="OMA" id="QITMKIL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd08321">
        <property type="entry name" value="Pyrin_ASC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="101" mass="11918" checksum="38E70C98406B36BA" modified="2024-11-27" version="2">MENIRRQLLDILEELLSDDLKKFQWHLTNGVEGFRHIPKSLMENVNRFNTVDTMVERYPQNGAVEITLKILNQMNQNELAEKLRTNLRESNAESWSRLSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CF40</accession>
    <name>A0A3B4CF40_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">POF1B helix-loop-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">POF1B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017559815.2">
        <property type="nucleotide sequence ID" value="XM_017704326.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CF40"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016736.2">
        <property type="protein sequence ID" value="ENSPNAP00000010103.2"/>
        <property type="gene ID" value="ENSPNAG00000015661.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431286"/>
    <dbReference type="GeneTree" id="ENSGT00390000000141"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003382">
        <property type="term" value="P:epithelial cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="POF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056240">
        <property type="entry name" value="POF1B_HlH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22546">
        <property type="entry name" value="PREMATURE OVARIAN FAILURE, 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22546:SF0">
        <property type="entry name" value="PROTEIN POF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24617">
        <property type="entry name" value="POF1B_HlH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="187"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010103.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="526" mass="59953" checksum="9AD722594DCB2B5E" modified="2024-11-27" version="2">MTLPSAQSTATLRTVSVASEPAVATVLSPGQFNINGASTLRTVSLAPGSPLQSVQYVNGTVDGNLLQGNVRYLVPVQRSAESYVVLNQTPAMVNPVYLQNMQNVQTVQRVSVSSVEETDSLQRLNINGKASSVFSQTSSPTKSPEPPEVVESTDVRMQSLNVDLKNELKSEVKTEVVVEPLDKLDTRFFGELLAEVYRKNCDIHSCISEHVAKIRGRKHLLDPTINYKVDKEEIEALIPKALSELTKQQIRYLLQTRLTADKTMRLLLTTFSSLREELVHLQDDLRRLESEKEELERDLTFKAEQTHQYDRLLDAVRENNRQLQNSLKETTTTQRTLESQLMISRSTDSSKDFRIKELQGSKRALEQENELLRKKLEGQCNTSTLNDKTKELSKHYEQMLSALREEKEKELKSMRSQLVKIQTEYTTVHSSDKSLQMRITELLTSLEQRESVIRHQEEEIRRLQQEKSDSSKSITKTVITKRYRNQYPILGLLSDDYQVTSPVKEAKTIVIERTGEMIKQEIITTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CIV5</accession>
    <name>A0A3B4CIV5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">von Willebrand factor A domain-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">VWA8</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Exhibits ATPase activity in vitro.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000011927"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036314.2">
        <property type="protein sequence ID" value="ENSPNAP00000011927.2"/>
        <property type="gene ID" value="ENSPNAG00000017819.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006601"/>
    <dbReference type="OMA" id="GTHIVHP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030903">
        <property type="term" value="P:notochord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000587">
        <property type="entry name" value="von Willebrand factor A domain containing 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000663">
        <property type="entry name" value="von Willebrand factor A domain containing 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039891">
        <property type="entry name" value="VWA8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21610">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21610:SF9">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="1709"/>
            <end position="1891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1539"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1539"/>
            <end position="1551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011927.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1900" mass="212196" checksum="C21F8F2CB58C43A8" modified="2024-11-27" version="2">MHARILFKGTVAAVAARRIRTILGPVAAKEGWKYSAREVKLLNTASGDTVKIGDVSFQLKAPRNPELVPVNHIPESMPQAVAQHLRWIMQKDLLGQDVFLIGPPGPLRRSIAMQYLELTKREVEYVALSRDTTETDLKQRREIRSGTAFYIDQLCVQCAVRAATEGRILVLEGLEKAERNVLPVLNNLLENREMQLEDGRFLMSAQRYDKLLQEHTKEELDLWKIVRVSEDFRVIALGLPVPRYKGNPLDPPLRSRFQARDVYYLPFKDQLDLLYNIGQNVPAERVSQLLSCATTLCSQESANLGLPDFPVDNLLPAVKVLDLFPMLSAQQLIQRLYPYEAMLGKEGRNAVEGVLSRFELLDGKRQSSPRAVVHVEPGHDTNTGQATVTLNIADKNITFQVPAGARPPRPPNSSPAFISTPSHARQLAEMMQSHLVKDMCLIGAKGCGKSVIAREFAEMLGYNTEQIMLYQDMTARDLLQQRYTLPNGDTAWRASPLVSAAQEGKMVFLDGIHRVNLGTLAVLSRLLHDRELALYDGTRLLRWDRYQSMKEELQLTDSELQDRCIFPIHPSFRVIALAEPPVLGSSSQQWLNPELLTMFFFHTVKPLTKSEEAAVIHGMTPNVPNEAVEQLLHLTHTLRRTNDPTAQSLASSLSTRQLLRICRHLSQYPEESIAHAVNKACLSRFLPSLARSSLQKNLADCSIEEHADLAAEQKQYHTSVVRDGVLTIGKVSAPVYSPDEKMKVPDVLFYENAQHMMVMEDMLKDFLLGEHLLLVGNQGVGKNKIVDRFLHLMNRPREYLQLHRDTTVQTLTLQPSVRDGIIVYEDSPLVKAVKMGHILVIDEADKAPTNVTCILKTLVESGEMILADGRRIVSDPLEAVARPNVLAMHPDFRMIVLANRPGFPFLGNDFFGALGDIFSCHAVDNPKPHAEFAMLKQYGPDVPDAVLQKLVAAFGELRAMADQGNITYPYSTREVVNIVKHLQTFPEEGLANVVRNVFDFDSYNKDMREVLIAVLHKHGIPIGAKSTSVHLAKELPLPDGKMTGYWSINQGGSPRRKLLCPTESHRIDIKGPVFLRVQGYPLERHESRSMSFTEEHAHWQIPMNEVNIVCDVTTANDVIFVATCNPVALYSMREQADTLHCIELYDVFPRTISGVWQPFITVAALGSPLQGQVVLHEEQSNTVLHVDLVTGAVRRLVLSPGKDEETERKTSSWWNSKDSQAGYRMCREFAHKNWLLFYKADGNQLDVLDVLEGQVHTISLPINLKSVFLVAEDRWLLTESKTDRKFLLTKPMHMGAEESGVCQLHTISEEAVSTGFGNRTVMEPAAPQEVSSEQLPNENLSTALGQKIVSPNRLVCDTHTYASVVVGFPDLLSLNEVYSFKRTSPLTEGRGPDMFFGSSRSTGPAKRVNCVSLVGANQVVRALPPTQVPLADIYPKDITPPTTAAYLEVTDLNSKKVKYIPVPRTTSMSPYTVWISKVSDTDVVMAPLGTGGVVTVDMGGYVRLWETGLDHLQRSLLEWRNMIGSEDGRPVQITIQRDSGHDVTSPKHGKIDATNAPHVGGNQWAGGTGGRDTAGLGGKGGPYRLDAGHKVYQVSQAEKDAVPEEVKRAAREMAEKAFKQRLKEIEMSEYDATTYERFSGAVRRQVQSLRIILDSLQAKGKERQWLKNQALGELDDAKIIDGLTGEKAIYKRRGELEPELGTPQQKPKRLRLLADVSGSMYRFNGVDGRLERSMEAVCMVMEALESYEHKFKYDIVGHSGDGFDIELVRCEKVPKNNKQRLKVLKTMLAHSQFCMSGDYTLEGTEHAVMELAREEADEHFVIVLSDANLERYGISPDRFARALTSNPQVHAFAIFIGSLGDQADRLQRTLPAGRSFVAMDTKQIPQILQQIFTSTMLSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CMS1</accession>
    <name>A0A3B4CMS1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">RNF215</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZNRF3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017540570.1">
        <property type="nucleotide sequence ID" value="XM_017685081.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CMS1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011854"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036282.2">
        <property type="protein sequence ID" value="ENSPNAP00000011854.2"/>
        <property type="gene ID" value="ENSPNAG00000017539.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412854"/>
    <dbReference type="CTD" id="200312"/>
    <dbReference type="GeneTree" id="ENSGT00940000159671"/>
    <dbReference type="OMA" id="GAQAYPK"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16670">
        <property type="entry name" value="RING-H2_RNF215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000031">
        <property type="entry name" value="RING finger protein 215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040700">
        <property type="entry name" value="ZNRF-3_ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051073">
        <property type="entry name" value="ZNRF3_Arkadia_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16200">
        <property type="entry name" value="RING ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18212">
        <property type="entry name" value="ZNRF_3_ecto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043825887" description="RING-type E3 ubiquitin transferase" evidence="19">
        <location>
            <begin position="28"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="321"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="57"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011854.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="370" mass="41340" checksum="9985DAA4F9737BD9" modified="2024-11-27" version="2">MGASGGCQRCVSLLLAACLWLSGAVWAEQVALVEVFVQQDSVRILQGQLLQDSLDANPAQTHHKKDHKEPDLQGDLRLIRDEDEGNEVISRDDEDDDGDGAEEKKKNLWIGVVPVEMEESSTVSNGNQESFAAAVVNKMKRALVLGASALIILVLNQNTVREMDLSQALSKPVIIIQTSENVTRLIGALLRGLHATAKITYKTFLQNNLGATLTLWSSCGRSRGGLYGEWQGVICTGETNSPVQKYLQQLWNTVVLVALILSTGVIVHARWQYHYNQFNDDLQLRPKQDLLKRLSALKTRTYRQPKAWSDPTHGQPETDSCAVCLEQYNNNQCLRVLPCLHEFHRDCVDPWLLLQQTCPLCKRSVLGNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CRI8</accession>
    <name>A0A3B4CRI8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017575159.1">
        <property type="nucleotide sequence ID" value="XM_017719670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CRI8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013371"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020944.2">
        <property type="protein sequence ID" value="ENSPNAP00000013371.1"/>
        <property type="gene ID" value="ENSPNAG00000019204.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440664"/>
    <dbReference type="CTD" id="557905"/>
    <dbReference type="GeneTree" id="ENSGT00500000044852"/>
    <dbReference type="OMA" id="VTLCKAP"/>
    <dbReference type="OrthoDB" id="10057496at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF91">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 33B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="503" mass="57055" checksum="B07172B9A859246E" modified="2018-12-05" version="1">MVLITGDCPRGGLPVVQDQNGSTDGQVGACRAFAESSVQRSNGNEEHSSGDDEYFDDDDDDVYQEFEEFDFSQLPDSKSIASDDSFYPPDESITYHRSPLAESPEPLTFFKACCNNNAIIVKIMIRQGVTEEEVREVDKNKRTGLIVACYQGYVDVVIALTQCPYVNVNWQDNEGNTALITAAQAGHIMITNYLLNYFSGLDIERRNCHGFTAMMKAAMQGRADCVRALMMSGADIEARDYGRKLTPREWALFTGRHETARVILRLMAQPCAEQFCDSFSMEWPALRELVEQAQEPKSCWRTISERACAAFVFRTKTEPLEEGVLDYMVRMTTALASPLVAIACHTVCPGSPPCVGKRRPPVPDILRKQRLDELRRLGPERLSNYKRLFQNSRVLLVPKKRERRASLQPPALHDMALASTVALRRSSLLPLHMMRRSSVRPGMVVPKVRLCKAPPPTYVPERIRHRGDSQYLQVPKWKYKALKEERKQMEQGNKLKFPVVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTH7</accession>
    <name>A0A3B4CTH7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="14">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Long-chain-fatty-acid--CoA ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SLC27A6</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017546804.1">
        <property type="nucleotide sequence ID" value="XM_017691315.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTH7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014056"/>
    <dbReference type="Ensembl" id="ENSPNAT00000021847.2">
        <property type="protein sequence ID" value="ENSPNAP00000014056.1"/>
        <property type="gene ID" value="ENSPNAG00000019955.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423774"/>
    <dbReference type="CTD" id="28965"/>
    <dbReference type="GeneTree" id="ENSGT00940000159700"/>
    <dbReference type="OMA" id="VWRQFLD"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000002">
        <property type="entry name" value="Long-chain fatty acid transport protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000005">
        <property type="entry name" value="Solute carrier family 27 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006134">
        <property type="entry name" value="PRK08279.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF10">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="18">
        <location>
            <begin position="62"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="622" mass="70583" checksum="7CE557EE57EA926B" modified="2018-12-05" version="1">MFFSVTWATALLAGIVSVYIAQRLCFPYFWKDVFYLLKVIRYGARLEFYKRTKIVTVLDRFVQQARRIPDKPFLIVEGVSYTYRQIDERSNRLARVFEQQGSVKRGDTVAMLMSNEPDFVSVWFGLSKLGCALAFLNTNIRSRSLLHCFNSCGAKLLVVGADLLDTLKEILPSLHEDNISVWAMKHEVSHPGVYSLLDKIEQASHEPMPAELRSTTSLKTPLLYIFTSGTTGLPKAAVITHLQSLKAAGGFWAYGGTAEDVIYTPLPLYHSAASLVGIGGTIELGATCVLKKKFSASQFWNDCRKYDVTIFQYIGELCRYLCNQPKAENEKDHKVYMGVGNGLRQDVWREFHERFGKIRMCELYGSTEGNLCFMNHIGKIGAVGRSNFFYKLLFKYDLVKYDMLKEEPVKDEQGFCLRVKRGEMGLLLSKISSQSPFFGYAGSKQLTENKVLRNVFVKGDAYFNTGDLMVEDEENFISFKDRVGDTFRWKGENVATTEVTEVLGLPDFIQEANVYGVEVPAHEGRAGMAAIIVRPSFEFDGKKLFEAVLTHLPTYARPLFIRLQESMEMTGTFKQQKFRLVKSGFNPSTISDPLYFLDYTEKSYVPLTDAIYESIRAGQRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CTW3</accession>
    <name>A0A3B4CTW3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">CTTN</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell projection</location>
            <location evidence="10">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
            <location evidence="9">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <location evidence="11">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CTW3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015532"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023762.2">
        <property type="protein sequence ID" value="ENSPNAP00000015532.2"/>
        <property type="gene ID" value="ENSPNAG00000021569.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440597"/>
    <dbReference type="GeneTree" id="ENSGT00940000158565"/>
    <dbReference type="OMA" id="CAKTWEY"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11959">
        <property type="entry name" value="SH3_Cortactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000087">
        <property type="entry name" value="Src substrate cortactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035716">
        <property type="entry name" value="Cortactin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003134">
        <property type="entry name" value="Hs1_Cortactin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF15">
        <property type="entry name" value="SRC SUBSTRATE CORTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02218">
        <property type="entry name" value="HS1_rep"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51090">
        <property type="entry name" value="CORTACTIN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="19" id="KW-0965">Cell junction</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="25" id="KW-0966">Cell projection</keyword>
    <keyword evidence="23" id="KW-0168">Coated pit</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 26" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="20" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="SH3" evidence="28">
        <location>
            <begin position="479"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="318"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="27">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="351"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015532.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="537" mass="60284" checksum="7AA796D6C6F119DA" modified="2024-11-27" version="2">MWKAAAGRSVSVTVDEGADDWETDPDFENDVTEKEQRWGAKTVEGSGHQEHINIHHLREKVSCEHSELKQKELANMPKASHGYGGKFGVQQDRMDKSAVGHEYQSKLSKHCSQTDTSKGFGGKYGVQTDRVDQSAVGFEYVGKTEKHASQKDYATGFGGRYGVQADRVDESAVGFDYQGKTEKHESQKDYTKGFGGKFGVEMDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKSAVGWDHQEKLQLHESQKDYKRGFGGKYGVEVEKQDQCALGFDHKESLAKHESQQDYSKGFGGKYGVQKDRMDKSAGTFEEVEKPSAAYQKTRPVEAAGSGTGNIKARFENLAKQKEEEDKKRAEEERARRQAKELQEQEEARRKIEESAKARAPSPQPSPTTTTAPEYKEAHSEIEEKEDNVYEVEPVSAPASKPIYDEPVQEDLYQNQSQVLEHEQDGQYQAADTQSYDYGEDLGVTAVALYDYQAAGDDEISFDPDDIITNIEMIDEGWWRGVCRGAYGLFPANYVEVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVF8</accession>
    <name>A0A3B4CVF8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Asparagine-linked glycosylation protein 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ALG5</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017572604.1">
        <property type="nucleotide sequence ID" value="XM_017717115.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVF8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014509"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037349.2">
        <property type="protein sequence ID" value="ENSPNAP00000014509.1"/>
        <property type="gene ID" value="ENSPNAG00000020464.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438965"/>
    <dbReference type="CTD" id="29880"/>
    <dbReference type="GeneTree" id="ENSGT00940000153481"/>
    <dbReference type="OMA" id="HMVNTDA"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000068">
        <property type="entry name" value="ALG5, dolichyl-phosphate beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="16">
        <location>
            <begin position="75"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="331" mass="37164" checksum="9BFD7F49DC3B7099" modified="2018-12-05" version="1">MELELGLGLGLGLCQLLQAAAVLALLLLLLVIVIAHATAGMVDQTRAEQEKHFLTASGNKEPFPSLFDPHSRELSVVVPSYNEESRLPVMMEEAMEYLEKRQKKNPSFTYEVIIVDDGSKDRTTEVALGYTKKYGADKVRVLTLKKNRGKGGAVRMGTLSSRGRLILMADADGATKFGDIEKVEAGLENLSPKPENMAISCGSRAHLEKDSVAQRSLFRTILMYGFHFLVWFFCVKGIKDTQCGFKLFTREAALRTFSSLHVERWAFDVELLYVAQCLKIPIAEVAVNWTEIEGSKLVPFWSWLQMGRDLVSIRLRYITGAWRLESPRKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZ81</accession>
    <name>A0A3B4CZ81_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 22">Dihydropyrimidine dehydrogenase [NADP(+)]</fullName>
            <shortName evidence="22">DHPDHase</shortName>
            <shortName evidence="22">DPD</shortName>
            <ecNumber evidence="5 22">1.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19 22">Dihydrothymine dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 22">Dihydrouracil dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>5,6-dihydrothymine + NADP(+) = thymine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17821"/>
            <dbReference type="ChEBI" id="CHEBI:27468"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:58286"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>5,6-dihydrouracil + NADP(+) = uracil + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15901"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:18095"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 22">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="22">Binds 4 [4Fe-4S] clusters. Contains approximately 16 iron atoms per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 22">Amino-acid biosynthesis; beta-alanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the dihydropyrimidine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.2" evidence="5 22"/>
    <dbReference type="RefSeq" id="XP_017555870.2">
        <property type="nucleotide sequence ID" value="XM_017700381.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZ81"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015859"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024177.2">
        <property type="protein sequence ID" value="ENSPNAP00000015859.2"/>
        <property type="gene ID" value="ENSPNAG00000021996.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428973"/>
    <dbReference type="CTD" id="118225829"/>
    <dbReference type="GeneTree" id="ENSGT00500000044896"/>
    <dbReference type="OMA" id="ISLWVRE"/>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00131"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017113">
        <property type="term" value="F:dihydropyrimidine dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002058">
        <property type="term" value="F:uracil binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019483">
        <property type="term" value="P:beta-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006212">
        <property type="term" value="P:uracil catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02940">
        <property type="entry name" value="DHPD_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000007">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000023">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000056">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000061">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43073:SF2">
        <property type="entry name" value="DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 22" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 22" id="KW-0274">FAD</keyword>
    <keyword evidence="8 22" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 22" id="KW-0288">FMN</keyword>
    <keyword evidence="16 22" id="KW-0408">Iron</keyword>
    <keyword evidence="17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 22" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="23">
        <location>
            <begin position="945"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="23">
        <location>
            <begin position="978"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU364041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015859.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1026" mass="112036" checksum="56C78C078FF07B23" modified="2024-11-27" version="2">MARMLSKDLPDIESILALNPRVKSHATLYSTAAKKAEKKHWKRNAEKGCDSCVKLENNFDDIKHTTLSERGALREAMRCLKCADAPCQKSCPTNLNIKAFITSIANKNYYGAARAILSDNPLGLTCGMVCPTSDLCVGGCNLFASEEGPINIGGLQQFATEVFSRMGIPQVRNPELPPPEQMPESYHSKIALIGCGPASISCASFLARLGYDNITIFEKQKYVGGLSSSEIPQFRLPYEVVQFELDLMKDMGVKVMCEKRLGQDGMTLLSLKEEGYKAVFIGIGLPQANRHRIFEGLSMEQGFYTSKDFLPLVAKASKPGMCGCGWTLPRLYGSVIILGAGDTAFDCATSALRCGAQRVYVVFRRGFTDIRAVPEEMELAKEEKCEFLPFLSPREVLLKEGRVHSMEFCRTEQTATGEWVEDQEQITRLKADFIISAFGSVLSDPKVKEAMAPIRLNSWGLPELNPETMQCSEPWVFAGGDVAGLANTTVESVNDGKQASWHIHRYLQAIHGHTFSSHPQLPLFYSPIDAVDISVQMCGIKFPNPFGLASAPPTTSASMIRRAFEQGWAFALTKTFSLDKDLVTNVSPRIVRGTTSGPMYGPAQGSFLNIELISEKTAAYWCQSVTELKVDFPHNIIISSIMCSYNKDDWTKLAKMAEASGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVKIPFFAKLTPNVTNIVDIAKAAHEGGADGVTATNTVSGLMGLKADGSPWPGIGRDKRTTYGGVSGNAIRPIALRAVSAIARALPGFPILATGGIDSAESGLQFLHAGASVLQVCSAVQNQDFTVIEDYCVGLKTLLYLKSIEELQDWDGQSPPTIRHQQGKPVPTLEELVGKRLPNFGPYLQEKKMAIANYKLKLANQSDNTVHPGGSRINTPSKPVPTVQEVIARALKHIGAYQELNNQEQVQALIDEEMCINCGKCYMTCNDSGYQAITFDPETHLPVVQDSCTGCTLCLSVCPIIDCIRMVARTTPYVPQRGLPQAIMPVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CZB3</accession>
    <name>A0A3B4CZB3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017577693.1">
        <property type="nucleotide sequence ID" value="XM_017722204.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CZB3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017427"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026226.2">
        <property type="protein sequence ID" value="ENSPNAP00000017427.1"/>
        <property type="gene ID" value="ENSPNAG00000023726.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108442247"/>
    <dbReference type="CTD" id="406765"/>
    <dbReference type="GeneTree" id="ENSGT00940000153544"/>
    <dbReference type="OMA" id="MLVHHAL"/>
    <dbReference type="OrthoDB" id="3638488at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21782">
        <property type="entry name" value="MobB_NDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05628">
        <property type="entry name" value="STKc_NDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000057">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000086">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000118">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="89"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
        <location>
            <begin position="383"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="467" mass="54191" checksum="4E4947D7DC00B800" modified="2018-12-05" version="1">MAMTGQTSCSSMSNHTKERVTLAKVTLENFYSNLISQHEEREMRQQKLEKVMDQEGLADEEKRIRRSQHARKETEFLRLKRTRLGLDDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQIGHIRAERDILVEADSLWVVKMFYSFQDKMNLYLIMEFLPGGDMMTLLMKKDTLTEEATQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAFSTVGTPDYIAPEVFMQNGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKVMNWRETLTFPPEVPISEKAKDLILRFCCESENRIGASGVEEIKSNPFFEGVDYDHIRERPAAIPIEIKSIDDTSNFDEFPDSDILQPTSTVVSNHSEVDLKNKDWVFINYTYKRFEGLTARGAIPSCMKSGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5Q2</accession>
    <name>A0A3B4D5Q2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="33">Dynamin-2</fullName>
            <ecNumber evidence="8">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="39 40">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="39 40" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="39" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="39" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Phagocytic cup</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Cell projection</location>
            <location evidence="32">Uropodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Cytoplasmic vesicle</location>
            <location evidence="30">Phagosome membrane</location>
            <topology evidence="30">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <location evidence="7">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="29">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Synapse</location>
            <location evidence="28">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="34">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5Q2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018244"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027352.2">
        <property type="protein sequence ID" value="ENSPNAP00000018244.1"/>
        <property type="gene ID" value="ENSPNAG00000024717.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155214"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001891">
        <property type="term" value="C:phagocytic cup"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001931">
        <property type="term" value="C:uropod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01256">
        <property type="entry name" value="PH_dynamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000019">
        <property type="entry name" value="Dynamin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000014">
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            <begin position="768"/>
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        </source>
    </evidence>
    <sequence length="855" mass="96213" checksum="C8318C3BCA14064C" modified="2018-12-05" version="1">MGNRGMEELIPLVNKLQDAFSAIGQNANLDLPQIAVVGGQSAGKSSVLENFVGKDFLPRGSGIVTRRPLVLQLINCPTEYAEFLHCKGKKFTDFDEVRQEIEAETDRITGQNKGISPVPINLRVYSPNVLNLTLVDLPGMTKVPVGDQPADIESQIRDMIMQFVTKENCLLLAVSPANSDLANSDALKIAKEVDPQGLRTIGVITKLDLMDEGTDAREILENKLLPLRRGYIGVVNRSQKDIDGKKDITAAIAAERKFFLTHPSYRHLAERMGTPYLQKALNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPSRKTKALLQMVQQFAVDFEKRIEGSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRKEISYAIKNIHGIRTGLFTPDMAFETIVKRQIAKIKEPCQKCVDLVISELVNTVRQCTKKLAQYPMLREEMERIVTQHIRDRESRTKTQVMLLIDIELAYMNTNHEDFIGFANAQQRSSQMSKKKAAGNQDEIMVIRKGWLTINNIGIMKGGAKEYWFVLTAETLSWYKDDEEKEKKYMLPVDNLKLRDIEKSFMSSKHIFALFNTEQRNVYKDYRQLELACETQDEVDSWKASFLRAGVYPERITQSEASEENGSESFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIHADLLAQLYSCGDQNTLMEESAEQAQHRDEMLRMYHALKEALNIIGDISTTTVSTALPPPVDDSWLQVQGGPSGRRSPMSSPTPQRRAPPGPPRPGGRTAPGPPAAGAPPVPSRPGASPDPFSGPPPQVPSRPNRAPPGVPSRPNKGSPSHGESPHPSFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5Y3</accession>
    <name>A0A3B4D5Y3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KPNA3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5Y3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018536"/>
    <dbReference type="Ensembl" id="ENSPNAT00000027907.2">
        <property type="protein sequence ID" value="ENSPNAP00000018536.1"/>
        <property type="gene ID" value="ENSPNAG00000024925.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244891"/>
    <dbReference type="OMA" id="TVELRKX"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000004">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000009">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="15">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="114"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="12">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="521" mass="57311" checksum="32301E3BA0D46397" modified="2018-12-05" version="1">MAENGALENHRIRSFKNKGRDAEAMRRQRNEVSVELRKNKRDEHLLKKRNVPQSESLDDSDVDSDLKGQNVTLETILQNASSNNNVVQLSAVQAARKLLSSDRNPPIDDLVRSGILPVLVKCLDRDDHPSLQFEAAWALTNIASGTSAQTQAVVQSNAVPLFLKLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGAVKPLLSFICPTVPLSFLRNVTWVIVNLCRNKDPPPPMETVQEILPALCVLIYHTDINILVDTVWAVSYLTDGGNEQIQMVIESGVVPFLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQAVLNCDVLAHFPTLLTHPKDKINKEAVWFLSNITAGNQQQVQAVIDAGLIPMVIHQLTKGDFGTQKEAAWAISNLTISGKKEQVEYLIQQGVVAPLCGLLEVRDTQVVQVVLDGLKNILLMAGEQASAVTVIIEECGGLEKIENLQQHENEYIYKLAFEIIDMYFSGDDIDEDSSLVPEATQGGTFTFSTSGDLQTDDFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D929</accession>
    <name>A0A3B4D929_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Solute carrier family 43 member 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-isoleucine(in) = L-isoleucine(out)</text>
            <dbReference type="Rhea" id="RHEA:70943"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-leucine(in) = L-leucine(out)</text>
            <dbReference type="Rhea" id="RHEA:73011"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-methionine(in) = L-methionine(out)</text>
            <dbReference type="Rhea" id="RHEA:70939"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-phenylalanine(in) = L-phenylalanine(out)</text>
            <dbReference type="Rhea" id="RHEA:27950"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547571.1">
        <property type="nucleotide sequence ID" value="XM_017692082.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D929"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029577.2">
        <property type="protein sequence ID" value="ENSPNAP00000019616.1"/>
        <property type="gene ID" value="ENSPNAG00000026246.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424209"/>
    <dbReference type="CTD" id="334395"/>
    <dbReference type="GeneTree" id="ENSGT00940000153576"/>
    <dbReference type="OMA" id="RMSFDAF"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072015">
        <property type="term" value="P:podocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000274">
        <property type="entry name" value="Solute carrier family 43 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20766:SF0">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20766">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="520"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="520"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000019616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="60765" checksum="645B4882F4C6A2FF" modified="2018-12-05" version="1">MKGMAPSLTQAYRRRWWMACTAVLENLCFSAVLLGWGSLLIMLKKERFYSHLCTKNETGVPDVSHTNTSQEGLSCVEQEEVLNLGFTIGSFLLSAATLPLGILMDRYGPRPLRLTGSACFALSCAMMSVSAYNPEVLSALIFLALSLNGFGGICLTFTSLTLPNMFGSLSSTVMSLMIGSYASSAVTFPGVKVIYDAGVSFTVIMWVWAGLATLVLLNCFLNWPVESFPTPQELEEMQKMRSGDDAQEQMNHSTDKLTEATQNTTNTIPFRQSVFSPIFLWSLVTMGMSQLRIIFFMGAMNKMLEFLVTHGKSHPSGELVTDAKEKVSFYSSIFGTLQLLCLVTCPLIGYIMDWKMKECEEETAPAKRDQQCSSACKPNRDRKIQKLTNAMRAFTFTNLLLVLFGITSLIDNLPLQILTFILHTMVRGFIHSCCGGLYAAVYPSNHFGTLTGLQSMISAVFALLQQPLFIAMVGHWEGDPYWINVGLLIFSLAGFLLPGYLLYYRKHLIQERAAQDKATGQTAAESQVLNQTDTKGINHHSNGHIPQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DB47</accession>
    <name>A0A3B4DB47_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GPR27</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DB47"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000020179"/>
    <dbReference type="Ensembl" id="ENSPNAT00000039880.2">
        <property type="protein sequence ID" value="ENSPNAP00000020179.2"/>
        <property type="gene ID" value="ENSPNAG00000005514.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00890000139436"/>
    <dbReference type="OMA" id="CIFEHRH"/>
    <dbReference type="OrthoDB" id="8898476at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051509">
        <property type="entry name" value="GPCR_Orphan/Phoenixin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19268">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19268:SF8">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 27-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="46"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020179.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="365" mass="38040" checksum="028028FE6A821685" modified="2024-11-27" version="2">IAGGGEGGSAALLADKTDDPGGPPLPLQDSVQLAALVAMLCASVAGNALLCALVLKDSALHRAPYYLLLDLCAADAARAALGFPVAAATATAGALLAALFCFHVAFVLLAASVTRYLAIAHHRFYAKRVTPCACAALVAASWALSAAAALPPALEQSAGAFPRDPEHCALGHAPARAGDALGLALTLACVVGATHAVYAKILCFVYGHRKMKPAQPVPAVSHNWTFHGPGATGQAAANWIAGFGRGPTPPALVGVRQAPRDAGRRLLVLDEFKTEKRIGKMFYILTLAFLLLWAPYVAACYARVFAEGGAVPHVYLTAAVWLTFAQAAANPIICFAFNKELRVRLRACFPCCVTTQTPMEPYCVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DC21</accession>
    <name>A0A3B4DC21_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Purkinje cell protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCP4 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSPNAP00000021957.1"/>
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        <property type="component" value="Chromosome 18"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calmodulin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Calmodulin_Regulator_PCP4-like"/>
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        <property type="entry name" value="CALMODULIN REGULATOR PROTEIN PCP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15359">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="42"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038017"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7022" checksum="2EE088E84CF963BC" modified="2018-12-05" version="1">MSERQGPGASTGNSKPLGDQDASKKDVPADDFDIDMDAPETEKAAVAIQNQFRKYQKKKHDVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DD66</accession>
    <name>A0A3B4DD66_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSPNAG00000028006.2"/>
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    <dbReference type="OMA" id="FCRTGMN"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021006.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="192" mass="21518" checksum="49538C27613B3548" modified="2024-11-27" version="2">MTGASWSEHKYNLLKLAGQETEQEPRDGEPPKDAQVNGDIIRVGTSDSLLDDKVIRTEVEKEIPMCVSLPIEVISHDQTFPFLNTTLADLGIEESAVKERLVWVDSKRTQVRSKSGKLKEKEVTVLEVRVKAQPPGQAQCQEVLYSTESHTDRAYCRSGVDILPWINTQPGEEQEDTFDDCSPSVGVQFEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DE18</accession>
    <name>A0A3B4DE18_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017572283.1">
        <property type="nucleotide sequence ID" value="XM_017716794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DE18"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021735"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033315.2">
        <property type="protein sequence ID" value="ENSPNAP00000021735.1"/>
        <property type="gene ID" value="ENSPNAG00000028873.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438767"/>
    <dbReference type="GeneTree" id="ENSGT00940000164108"/>
    <dbReference type="OMA" id="ADSATHQ"/>
    <dbReference type="OrthoDB" id="6151507at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14694">
        <property type="entry name" value="bZIP_NFIL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047229">
        <property type="entry name" value="NFIL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047106">
        <property type="entry name" value="NFIL3-like_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284:SF6">
        <property type="entry name" value="HYPOTHETICAL LOC799271-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284">
        <property type="entry name" value="NUCLEAR FACTOR INTERLEUKIN-3-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="175"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="346" mass="38518" checksum="313AFAF92361E51E" modified="2018-12-05" version="1">MSTFRNPGHPAEMCLPTSLADMTGEEDESISARRLVPLPGPSLLARRLLNLHSYRGSLIPSRRRKREMTPAEKKDALYWDKRRKNNEAAKRSREKRRFNDLMLEGQLLALSEENAQLRAELLSLQYHMGLGRGADIAHALHSNAPFQCPTQPHLQSSLWGLSAAIPPTDLYQCWPQSSSCSASPPTNSSQILSQKNPQRNGTVPPHAPSMDQPAEQSTQGSRSSEADSATHQQVSSTNDPTNEQESSSSNSPSSPSSLTTQPTQNWLLTGLKHPTNQHNNLLLQWGSSCLRPSPLHACWPQSFSLPFRDSDESHNGINSFWNINGKFSMLSAEISQLRRYLRPENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DKP9</accession>
    <name>A0A3B4DKP9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DKP9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024952"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008832.2">
        <property type="protein sequence ID" value="ENSPNAP00000024952.2"/>
        <property type="gene ID" value="ENSPNAG00000031071.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182977"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF87">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="52"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024952.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="264" mass="29230" checksum="C077FB3176943421" modified="2024-11-27" version="2">MAVPPISTRAVCTQVAKLAVLFTTGLVEVRLAGLANRWLTGCSEYPLNRGAVKRGGSLTFPCFYDEKYKSNHKYWCKGQDWLLCKIVAYTNTSGRTSVTDHPTQNMFTVELNSLQDSDSGYYWCAVEISDGSDDGDYVYLTVSSGKTPSSKITSTTAVTPVSATEKTSINETTSGLITNRGLQLIKSRNKKNEYVCFVLLVSETMTKNTETTTAELCCAFTPQYNMFLLFPFSRTTAPPAVTASEKENDTSKSGKIQYKYVKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DS81</accession>
    <name>A0A3B4DS81_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037391525.1">
        <property type="nucleotide sequence ID" value="XM_037535628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DS81"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006312.2">
        <property type="protein sequence ID" value="ENSPNAP00000025956.2"/>
        <property type="gene ID" value="ENSPNAG00000011250.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413082"/>
    <dbReference type="GeneTree" id="ENSGT00940000162297"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16269">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000422">
        <property type="entry name" value="Guanylate-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1000.10">
        <property type="entry name" value="Guanylate-binding protein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037684">
        <property type="entry name" value="GBP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="8">
        <location>
            <begin position="39"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="476"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025956.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="585" mass="66910" checksum="BA73FD627EBC5E4C" modified="2024-11-27" version="2">MSCSKSKIDMPHPVCLITNTEDGGLLICQEGIDILDQIIQPVVVVAVVGPYRTGKSYLMNRLAGKRKGFALGSTIESKTKGIWMWCVPHPHIEDHTLVLLDTEGLGDVDKGDEKHDVWIFCLAVLLSTTLVYNSLGTIDNTALEKLQYVTKLTEHIEVKSRTEDGESMELMRVFPSFVWTVRDFTLDLELDGKEITADEYLENALMLKKGDTVTPNVVNYNKTRRSLLDLFAVRKCFVFDRPGNAKTMKQIEKLADDDLDESFVEQSMAFCTYIYTNSKSKTMRGGRSVTGRMLATLAQTYVKAISSGQVPSLDNAVESLAQIHNSRAVQEAVEFYRCEMTKRVHFPTETQQELSDIHAGVEKEAISIFIKGSFNDQNQEYQKDLVKCLSKEYEELVKQNMEESRKVCRSIITRVFGSLEEEVKKGIYMRSGGYIEYCSALERAVQQYRSEKGHGLMVEQVLTNYLAENNNVGENILAADRSLSEAEREIEEERREKEALEQKNRQLEEQKAIQEKLFLDEKNSLRENIRQLTVKKEEEERRSREEIMRLQQAIHQGRRSPWNSFTSFLVNDVIHPATSWISKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DV41</accession>
    <name>A0A3B4DV41_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thromboxane A2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Prostanoid TP receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PTGFR</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DV41"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027338"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002825.2">
        <property type="protein sequence ID" value="ENSPNAP00000027338.2"/>
        <property type="gene ID" value="ENSPNAG00000012814.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234559"/>
    <dbReference type="OMA" id="ILGHRNY"/>
    <dbReference type="OrthoDB" id="8949984at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004958">
        <property type="term" value="F:prostaglandin F receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004960">
        <property type="term" value="F:thromboxane receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Thromboxane A2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Thbox_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF4">
        <property type="entry name" value="PROSTAGLANDIN F2-ALPHA RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00429">
        <property type="entry name" value="THROMBOXANER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="193"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="37"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017628"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027338.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="347" mass="39328" checksum="AAFE13A8FA9081BE" modified="2024-11-27" version="2">PWKTGTSSYPISTTVKKYESRDVSVTTVVNMTVGIVSNTLAFFIVLKAYRRFRLKSKASFLLFASGLVLTDLLGHVMNGSLALYVYASDKDWESFDPKQILCGFFGVCMTFFGLSPLLLGTIMAVERCMGVTRPLFHSMALTSNRINRLLALTWLLALLVALLPVLVQRPYQVQGSCSWCFFRMVGPRDWLDILLPMIFSVLGLLSLLVSFVCNSVTGFTLLWSRLHREHDYHRHHRSTIHFEMVCQLLAIMIVSCVCWGPLLVTIIIITLQRQDAHGHLLLTVRLATWNQILDPWVYILLRKAVLKRLLLLAQRCHSSRPKHAFGWRFSTLGSSVETSSTVNPLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXP7</accession>
    <name>A0A3B4DXP7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DDE-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXP7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000027831"/>
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        <property type="protein sequence ID" value="ENSPNAP00000027831.2"/>
        <property type="gene ID" value="ENSPNAG00000005134.2"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF26">
        <property type="entry name" value="TIGGER TRANSPOSABLE ELEMENT-DERIVED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE-1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000027831.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="113" mass="12794" checksum="309FD83FEC656169" modified="2025-10-08" version="3">MEVYVLLVLDNAPEHPAHLDDCHPNATVVYLPPNTTALLQPMDQGVIASFKAYYLRRTIAMALGATEKNKDLTLKDFWKSYNIRDSVKNIADSWDEVKQTNMNGVWKNCVPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DXX1</accession>
    <name>A0A3B4DXX1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HDAC4</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DXX1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000391.2">
        <property type="protein sequence ID" value="ENSPNAP00000028275.2"/>
        <property type="gene ID" value="ENSPNAG00000013918.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157440"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10162">
        <property type="entry name" value="ClassIIa_HDAC4_Gln-rich-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000002">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046949">
        <property type="entry name" value="HDAC4/5/7/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF13">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037911">
        <property type="entry name" value="HDAC_II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="18">
        <location>
            <begin position="69"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="17">
        <location>
            <begin position="655"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="211"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="488"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="140"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="264"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="777"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="647"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="649"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="655"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="725"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Contributes to catalysis" evidence="15">
        <location>
            <position position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
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            <dbReference type="ARBA" id="ARBA00012111"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF00850"/>
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        <source>
            <dbReference type="Pfam" id="PF12203"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
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    <sequence length="1058" mass="117002" checksum="692311BF2A0AC092" modified="2024-11-27" version="2">NTPHARQIHHVHNGLPCKEQPLELLKPSGLNHIPTVDVSAALPIPVPPAAIPMDLRVDQQQHMSRASVDTGQCEQQLQQELLALKQKQQVQRQLLIAEFQRQHEQLSRQHEAQLQEHIKHQQELLALKHQQELLEHQRKLERHRREQELEKQQREQKLQQLKNKERGQESAVASTEVKMRLQEFVLNKKKALAQRSLNHCMPSDPRYWYGKTQHSSLDQSSPPQTGISTYNHPVLGLYDSKNDFPLRKTASEPNLKLRSRLKQKVTERRSSPLLRRRDGPIATAKKRSLDMAESACNSAPGSGPSSPNNSSNNITNENGITGSISNSSVEPSLAHRLAGREGPISQLSLYTSPSLPNITLGLPATGSSSVASSQQEGERLAMAGLQHGLPITTPFITGAHLPSYLATSALDREGGSTHNPLLQHMALLEQSASQNPLSGLSALPLQSPSIHKLRQHRPLGRTQSAPLPQNAQALQQLVVQQQHQQFLEKHKQQFQQQQLHMSKMMAKPSEPSRQHESHPEETEEELREHQAIIDPAEGLPRGVTIKQEPPDELEEELQQRERQAEQELLFRQQALLLEQQRIHQLRNYQASMEAAGLSVTFPGHRPLSRAQSSPASATFPIVVQEPPAKPRFTTGLVYDTLMQKHQCMCGNTNTHPEHAGRIQSIWSRLQETGLRGKCECIRGRKATLEELQTVHSEAHVLLYGTNPLRQKLDSSVTPMFVRLPCGGIGVDSDTIWNEVHSSSAARLAVGSVVELVFKVASGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAIAAKLLQQRLNVSKILIVDWDVHHGNGTQQAFYSDPNILYLSLHRYDDGNFFPGSGAPDEVGSGSGLGFNVNMAFTGGLDPPMGDAEYLAAFRTVVMPIANEFAPDVVLVSSGFDAVEGHPPPLGGYKLTAKCFGYLTKQLMGLAGGRLVLALEGGHDLTAICDASEACVSALLGNELEPLSDEVLQQRPSLNAVRSIEKVIEAHSKYWRCLQRSASTLAYSLVEAQKCETEEAETVTAMASLSVAAKHMGKRSEEEPMEEEPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZ96</accession>
    <name>A0A3B4DZ96_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZ96"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015244.2">
        <property type="protein sequence ID" value="ENSPNAP00000028778.2"/>
        <property type="gene ID" value="ENSPNAG00000003180.2"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="entry name" value="ALN"/>
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    <dbReference type="Pfam" id="PF17696">
        <property type="entry name" value="ALN"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000028778.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="74" mass="8495" checksum="5935ECF63601B87B" modified="2024-11-27" version="2">MGILKICGMSLKVLKGHKRNSSTQLGKRKLCCAEMAGVEKQGAVSLPKHSYWFDFWVFVLFDIAFFLFMYFVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E0T9</accession>
    <name>A0A3B4E0T9_PYGNA</name>
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        <recommendedName>
            <fullName evidence="13">Zinc finger and BTB domain containing 21</fullName>
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        <name evidence="11" type="primary">ZBTB21</name>
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        <name type="scientific">Pygocentrus nattereri</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_017682523.2"/>
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    <dbReference type="STRING" id="42514.ENSPNAP00000029325"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016328.2">
        <property type="protein sequence ID" value="ENSPNAP00000029325.1"/>
        <property type="gene ID" value="ENSPNAG00000015335.2"/>
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    <dbReference type="CTD" id="49854"/>
    <dbReference type="GeneTree" id="ENSGT00940000161028"/>
    <dbReference type="OMA" id="PMDIIRI"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="zinc finger and BTB domain-containing protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000065">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041011">
        <property type="entry name" value="Znf_C2H2_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF15">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18450">
        <property type="entry name" value="zf_C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="49"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="506"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="621"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="730"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="861"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="889"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="1027"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="798"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="913"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1052" mass="118301" checksum="316FE3D50DF8BDE4" modified="2018-12-05" version="1">MDVSESRGGETRSARTRAEMESLVHYSSPGHAISILGVLNEQRLRGHLCDVVLVVGENRYQAHRSILAASSEYFQSLFSRRDSENRAVIHLDFCEPDAFEIVLNYIYSSSLFVDKCSLAVVQELGYSLGIPFLTNIMSTRPQVSYCVSRKRLSFSEEDDGVYPNRSVIVRQSGMGATHRGTFSNLQGKAPKHTDTTSNETPPAQAFREQTHLSSGYVEPNEMGTNVPPVSTIANAQSEDGDSKPIISSLPSILKGHEYRSPSGRPQLTSSVSFSESVQLAGLHSDHTDDKTGVEKVEPMYDSKPVSGSQHGLHNQTIDRSGPLIKSLLRRSLSMDSPVPVFSPVLELKDSQSREQSVVKLAMTKTSGSPSPESKHQVSKHMPPLILRSKQHNALDKEGTQGAEVQVKTEPSSPLADPSEIIRITVGDNLPVSFKDFQTNFDEGPRYFSPSKRKMKLDGTSNPCKRSRVLNEHQFMHDDGTSGETPHNSNVDESNQEPGELRPSKMFKCWSCLKVFRSNAGLYRHVNMYHNPEKPYACDICHKRFHTNFKVWTHCQTQHGVVQNPAPSSSSYSVLDEKFQRKLIDIVREREIKKALIMKLRRNKQGLQAQPFAKKNRRSRSYGCPYCGKMFFFQSHFKLHMKVHTAERASIDAQDDDDLHERQDQAGHVKENQNKDVFPCRLCNQKLLSLAELEDHERACRYATVCPYCGLRFSNTLVKKDHEAHCKYKKLTCLECMRTFKSSFSIWRHQVEVHNQNMMNVREQLTQGHQDVNREAGDALRSIPPPSKESMARADLNREEAAYSDSSAPATFDSEDSSSYVPEDLSTGPSELRVKEEPQEAAVTDGEQTTAKPAPLEEAGVWPCEKCGKLFSSHKDLERHQELLCHIKPFICHICHKAFRTNFRLWSHYQSHMSSSEESGTRQVDRQPSSPSTPSPPLTIAIQDPPASRASPPKVTQAAVTSSEEESRRSKSPVSKPNRLEQEKSENGSGQHALGRSDSMDKPVASQESDTLFYHAPTLSALTFKRQYMCKLCHRTFKTAFSLWSHEQSHSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E239</accession>
    <name>A0A3B4E239_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E239"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029810"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017273.2">
        <property type="protein sequence ID" value="ENSPNAP00000029810.2"/>
        <property type="gene ID" value="ENSPNAG00000016145.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000198840"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029810.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="99" mass="10521" checksum="2EA56ADC8DD962F4" modified="2024-11-27" version="2">MTSSGGGIMQRRKGGGTFWSCKTPQTEFFGVSGCRDLKCKNCLWQGHLAKDCKNPRRCKSCGGEGHLAHSCPRQEATYAMVLAGAGGGSVAPQTVGRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E3Q8</accession>
    <name>A0A3B4E3Q8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4E3Q8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018274.2">
        <property type="protein sequence ID" value="ENSPNAP00000030328.2"/>
        <property type="gene ID" value="ENSPNAG00000014598.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287506"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052309">
        <property type="entry name" value="C-type_Lectin_Domain_Fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46490:SF6">
        <property type="entry name" value="ASIALOGLYCOPROTEIN RECEPTOR 1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46490">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 12 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="6">
        <location>
            <begin position="76"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030328.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="181" mass="21196" checksum="47BC7BA4F9C73323" modified="2024-11-27" version="2">PHAQSTYYLSGGSFSALINTTMRRYYRLTAVCLGLLCVILLAAITVLWIKFNNLTIDRDQLQTNNDNMKNQKQRCFRDSFYYISTEKKSWSESRKDCKERGADLVIINKQVRERERERERERYTWIGLRDVDTEGVWKWLSSAHDADCAVTGYKYTGSERVSTWADYSCDRPAVGICEKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E6N8</accession>
    <name>A0A3B4E6N8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sarcoglycan, delta (dystrophin-associated glycoprotein)</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sarcoglycan beta/delta/gamma/zeta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4E6N8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000030976"/>
    <dbReference type="Ensembl" id="ENSPNAT00000019544.2">
        <property type="protein sequence ID" value="ENSPNAP00000030976.1"/>
        <property type="gene ID" value="ENSPNAG00000018054.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158509"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048738">
        <property type="term" value="P:cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048742">
        <property type="term" value="P:regulation of skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014732">
        <property type="term" value="P:skeletal muscle atrophy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007525">
        <property type="term" value="P:somatic muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048798">
        <property type="term" value="P:swim bladder inflation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006875">
        <property type="entry name" value="Sarcoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039972">
        <property type="entry name" value="Sarcoglycan_gamma/delta/zeta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12939:SF6">
        <property type="entry name" value="DELTA-SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12939">
        <property type="entry name" value="SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04790">
        <property type="entry name" value="Sarcoglycan_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000030976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="306" mass="33988" checksum="1657AA2C3395A8A3" modified="2018-12-05" version="1">MPGRALAGHPRPEWRGKRMTQEQCTHRNNVQSTEKPHVYKVGIYGWRKRCLYFFVLLLMILILVNLALTIWILKVMNFTIDGMGHLRITERGLKLEGDSEFLQPLYAKEIQSRPGSPLFLQSSKNVSINILNGKNQLVSQLIAGSNGVHARGKMLEVKSSTGKLLFSADDREVVVGAERLRVMGAEGAVFSNSVETPHVRAEPFKELRLESPTRALYMEAPKGVQIQAEAGDIQATCRSDLRLESKDGEITLDARRIKLLRLPEGKASPSGTRQTVYEVCVCPNGKLFLSQAGTGSTCQISNNICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EFV8</accession>
    <name>A0A3B4EFV8_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LRRTM4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Postsynaptic cell membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the LRRTM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EFV8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000034179"/>
    <dbReference type="Ensembl" id="ENSPNAT00000025754.2">
        <property type="protein sequence ID" value="ENSPNAP00000034179.2"/>
        <property type="gene ID" value="ENSPNAG00000023293.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154909"/>
    <dbReference type="OMA" id="TWASVQK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000005">
        <property type="entry name" value="leucine-rich repeat transmembrane neuronal protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF120">
        <property type="entry name" value="LEUCINE-RICH REPEAT TRANSMEMBRANE NEURONAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043736218" evidence="14">
        <location>
            <begin position="28"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="390"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="583"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="615"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000034179.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="635" mass="70905" checksum="94017BD567C68BB3" modified="2024-11-27" version="2">MGSAMRRRWFVVPLLVQAWLSANFCYGERPCPRSCRCDGKIVYCESSAFRDVPKNLSGGCQGLSLRYNSLASLRAGQFVGLNQLIWLYLDHNYIANVDARAFQGIRRLKELILSSNKITLLHNTTFHLVPNLRNLDLSYNKLQALQPGQFQGLRKLLSLHMRSNSLKNLPSRLFQDCRNLEFLDLGYNRLRSITRNAMSGLLKLTELHLEHNQFSKINFSNFPKLYNLRALYLQWNRIRTMSEGLTWTWTSLQKLDLSGNDLQEVEPEVYQCMPNLQTLNLDSNKLVNVSQEAVSAWITLTMISLAGNMWDCGPAICPLVNWLINFRGNKETPMICASPKEVQGEKVMDAVEASGVCRVTLVTPSVVSSPSTLSSTPALNAALPFVPTLQSGGSRKGQGTSLQHPTPSRPPQSDVSPPEQDFEPVSFHKIVAGSVALFLSVTMILLVIYVSWKRYPSSVKQLQENSSSARKRRKKSRETERTLSTPLQEYYVDYKPSSSEATDVLVNGTGPCTYTISGSRECEVPHQMSALTFYRYEQPVVDYCQAHQPLRLNMSYEGPPQGLEGLDELVPRDRSTIQTVALPAVPPSGSGTLGPCSPPTIRAPGEGPSIPYPSTERSISYPTTERPTSTLTFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EGU5</accession>
    <name>A0A3B4EGU5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DIRP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LIN9</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4EGU5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035742"/>
    <dbReference type="Ensembl" id="ENSPNAT00000029183.2">
        <property type="protein sequence ID" value="ENSPNAP00000035742.1"/>
        <property type="gene ID" value="ENSPNAG00000025955.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003188"/>
    <dbReference type="OMA" id="WVENEIS"/>
    <dbReference type="OrthoDB" id="2339771at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033471">
        <property type="entry name" value="DIRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010561">
        <property type="entry name" value="LIN-9/ALY1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045831">
        <property type="entry name" value="LIN9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21689">
        <property type="entry name" value="LIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21689:SF2">
        <property type="entry name" value="PROTEIN LIN-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06584">
        <property type="entry name" value="DIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19438">
        <property type="entry name" value="LIN9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01135">
        <property type="entry name" value="DIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DIRP" evidence="5">
        <location>
            <begin position="113"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="331"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="521" mass="59242" checksum="66131930F6739EE0" modified="2018-12-05" version="1">MAEVESLLDERLSAKALVSLREASLASWSSPRQKESSMGKAVCSKRNFLLNEDDEDEEERLSNSKLRKKNRVTTTPQKFSALLDRKSTQELGLRLRNLLKLPKAHKWCIYEWFYSNIDRALFEGDNDFCLCLNESFPYLKTRKLTRVEWSTIRRLMGKPRRCSSAFLEEERAALNHRRQKIRFLQQRRTFTDISDCSDLPDHIPLPLVIGTKVTARLRGSLDGLFTGQIDAVDTSTSTYRVTFERSGSGTHTIPDYEVFSSEPPETMPLTAFAEKQRSIQNKVPQTPTQMPCSTSAEFMSPWGKLSGTEDDTLGGFPVKFLVQVTRLSKILSIKKDHIKQLREMNTEAEKQKSYSEPLKLEFQKKYASIVLDVEQLNKALNKVLNEVQQYSFKLLPDQCPFANDPSTALRRRCEGEAQDMVRQANKLPSGQRYVHSQNLTQLITNLTTLLLQIKSLAEGDDLNSFEFKSLTESLNDIKSSLDSSNVRCFQNNVEINVAHIQSGLCQTGNLHAFTANCTGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EJY6</accession>
    <name>A0A3B4EJY6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">poly(ADP-ribose) glycohydrolase</fullName>
            <ecNumber evidence="2">3.2.1.143</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the poly(ADP-ribose) glycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.143" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4EJY6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000035521"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028666.2">
        <property type="protein sequence ID" value="ENSPNAP00000035521.2"/>
        <property type="gene ID" value="ENSPNAG00000003932.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003652"/>
    <dbReference type="OMA" id="FVKCIPK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004649">
        <property type="term" value="F:poly(ADP-ribose) glycohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990966">
        <property type="term" value="P:ATP generation from poly-ADP-D-ribose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046372">
        <property type="entry name" value="PARG_cat_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048362">
        <property type="entry name" value="PARG_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007724">
        <property type="entry name" value="Poly_GlycHdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837">
        <property type="entry name" value="POLY ADP-RIBOSE GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12837:SF8">
        <property type="entry name" value="POLY(ADP-RIBOSE) GLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05028">
        <property type="entry name" value="PARG_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20811">
        <property type="entry name" value="PARG_cat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARG helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="PARG catalytic Macro" evidence="6">
        <location>
            <begin position="353"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR607724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000035521.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="610" mass="69620" checksum="B7487E06466A937E" modified="2024-11-27" version="2">MLLTSSPVTVPRRLSRRVHTLSLNLFFQQGWLREMPFTFLKIGRSKNEQMAQRNNSSQMSECLNHMDSAGEGGEGSGRRPPQEPSTKRRHSQSSSAENTSFDFGENQEDTYPIGRLKKDPDCHMQLDSLSGGPHHTVLIDTKEFYDRKLTPYKGQHVWDSNHVKLPPGLPKSNAEARWRAVQRALHNLTMSKPSVGDVEMAIKSYNHSHQMQWPFDSLYTYFKCLNKKEKNFSSVISKMASLALQLPDLIKHPIPMLKQHRSHAITMSQVQISCLLANAFFCTFPHRNATKPGSEYSNYPTINFSSLFGNTCKRKTEKLRCLFHYFSTVTEEGTKPDGLVTFERICIPLNELPTWKEQQETLSNLHVTANGTIEKEGSGMLQVDFASKFVGGGVLGRGLVQEEIRFLLSPELIVARLFTEKLAENECLKITGVQTYSVCSGYSDSFEWLCPHKDDTERDEWKRRYCQIVALDALSFKDPREQYTEQNLKRELNKAYVGFRGDPNIPSDYTPAIATGNWGCGAFNGDPRLKALIQMMAAAVAKRDLAFFTFRNKNQAQELQKMHKLLKAHQVTVGQLYLLLKKYCDDYSHYQHKKNVFEFVREMASPSSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4EQB4</accession>
    <name>A0A3B4EQB4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017541204.1">
        <property type="nucleotide sequence ID" value="XM_017685715.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4EQB4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037474"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040939.2">
        <property type="protein sequence ID" value="ENSPNAP00000037474.1"/>
        <property type="gene ID" value="ENSPNAG00000006228.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413274"/>
    <dbReference type="GeneTree" id="ENSGT01150000287001"/>
    <dbReference type="OMA" id="YYKIALF"/>
    <dbReference type="OrthoDB" id="9927220at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF14">
        <property type="entry name" value="SUCCINATE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="49"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="326" mass="37197" checksum="C4A89ABB1E7248CB" modified="2018-12-05" version="1">MQLHFTEQPSQDKISNSTMASNCTDVIPQLWKYYLSPMYALEFAIGSVSNLLVILGYIFCLPSWKSTNVYLFNLALSDLIFLCTLPHLSYTYAHGLSKIIPALCVANRYILHVNLYSSILFMVWVSVDRLLVIRHPLRNHFLLTCKGSLLVSLLTWVWVNIQVIPLIAFVIQDLELSEWTKCNDFGSLSETKGTLIYSLVLTVTGYVIPLLALFLSSLKMVSLLLTQEQVLGTSFQRPLKIVRAAAIMFLVLYTPLHVMRNVRIASQLSEATLSLCSKVYIEVVYIVTRPVAFAHSAVNPIFYFLMTDRFKELLQDKLRQIRRMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4ER54</accession>
    <name>A0A3B4ER54_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin 13b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017564148.1">
        <property type="nucleotide sequence ID" value="XM_017708659.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4ER54"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000037779"/>
    <dbReference type="Ensembl" id="ENSPNAT00000034617.2">
        <property type="protein sequence ID" value="ENSPNAP00000037779.1"/>
        <property type="gene ID" value="ENSPNAG00000029807.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433833"/>
    <dbReference type="CTD" id="449797"/>
    <dbReference type="GeneTree" id="ENSGT00940000157010"/>
    <dbReference type="OMA" id="CPPCAPI"/>
    <dbReference type="OrthoDB" id="5845060at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF203">
        <property type="entry name" value="TETRASPANIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000037779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22404" checksum="BB1728BD9D9A90E5" modified="2018-12-05" version="1">MSCSGFTCSKNCLCAINLLYVMVSMLMIGIAAWGKWFGLVSSFQVVGGIIGVGVFLFFVALAGLIGAIKHHQVLLFFYMIILFLVFVVQFSVSSACLAINKEQQNHLLEVGWNNSRSTQRDVEKSLDCCGFSHVDVNETCAAACFSHTKCDTCAAKIQEHAGEVLRFVGGIGLFFSFTEILGVWLTYRYRNQKDPRANPSAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IIP3</accession>
    <name>A0AAR2IIP3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP-binding cassette, sub-family C (CFTR/MRP), member 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ABCC9</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037398583.1">
        <property type="nucleotide sequence ID" value="XM_037542686.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSPNAT00000083669.1">
        <property type="protein sequence ID" value="ENSPNAP00000039485.1"/>
        <property type="gene ID" value="ENSPNAG00000003774.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108439416"/>
    <dbReference type="GeneTree" id="ENSGT00940000156680"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008281">
        <property type="term" value="F:sulfonylurea receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18591">
        <property type="entry name" value="ABC_6TM_SUR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18602">
        <property type="entry name" value="ABC_6TM_SUR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03290">
        <property type="entry name" value="ABCC_SUR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000005">
        <property type="entry name" value="ATP-binding cassette, sub-family C (CFTR/MRP), member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000006">
        <property type="entry name" value="ATP-binding cassette, sub-family C (CFTR/MRP), member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000197">
        <property type="entry name" value="ATP-binding cassette, sub-family C (CFTR/MRP), member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000394">
        <property type="entry name" value="ATP-binding cassette, sub-family C (CFTR/MRP), member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000388">
        <property type="entry name" value="ABCC8/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047080">
        <property type="entry name" value="ABCC9_ATP-bd_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF173">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01092">
        <property type="entry name" value="SULFNYLUREAR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="452"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="537"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="577"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1065"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1154"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1255"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="307"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="674"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="1021"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1344"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="961"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="965"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000039485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1581" mass="178478" checksum="C70DB7729E9DD058" modified="2024-11-27" version="1">MSLSFCGNDVNNSYSVEKGVLNNGCFVDALNLVPHVFLLFITFPILFIGWGSQSSKVQIHHNTWLHFPGHNLRWILTFTLLFVHVCEIAEGIVSNTWMKSNHLHLFLPAFMGFIAATTSVVYYHNIETANFPKLLLALFIYWVLAFITKIIKLWKFAEVGLGVEHLRFCITALLVVLYGLLMAVEVNVIIVRKYVFFANPQRVKPPEDLQDLGVRFLQPFVNLLSKATYWWMNPLIIGAHKRPIELKKIGKLPIAMRSLTNYLRLKDAYEEQRQNAEDLEKSPSIWRAMYRAFGRPILLSSTFRYMADLLGFAGPLCISGIVERLENNTEVVRMNKTEDLPFGVFFMSSTQLLQNTYVLAVLLFLALVLQRTFLQASYYVTIETGINLRGALLAMIYNKILRLSTSNMSMGEMTLGQINNLVAIETNQLMWFLFLCPNLWAMPVQIIMGVILLYYLLGFSALIGALVIVLLAPIQYLIATKLADTQKSTLDYSTDRLKKTTEILKGIKLLKLYAWENIFCDSVLETRGKELTSLRTFALYTSMSIFMNAAIPIAAVLATFVTHAYISQSRLSPAEAFASLALFHILVTPLFLLSTVVRFAVKALVSVQKLGEFLQSEEIGDDSWRNGDMPVSLESCKKHPGARNRKQPLRYQMDNYEQPLRRQLRPTETEDVAVKVNNGYFTWGSNMSTLSDINIRIPTGQLTMIVGQVGCGKSSLLLAMLGEMQTHSGKVYWSKLPDSEMLPDYLSEYDSDFSHEERSKNRYSVAYAAQKSWLLNATVEDNITFGSPFNKQRYKAVIDACSLQPDIDLLPFGDQTEIGERGINLSGGQRQRICVARALYQNTNIVFLDDPFSALDIHLSDHLMQEGILKFLQDDKRTVVLVTHKLQYLIHADWIIAMKDGSVLREGTLKDIQTHDVELYEHWKTLMNRQDQELEKDTEIESQTTLERKTLRRAFYSREAKNQIDDEDEEEEEEEDEDDNLSTTTSRRSKIPWKVCWCYLSSGGFLLVFLMVTSKLAKHSVMVAIDYWLAAWTSSAPNEQNSSFSLDANATNISETIRNAEHRSYVPVFVILCGAGIFLCLITSLSVEFLGLSAATNLHHNLLNKIIHAPLRFFDITPLGQILNRFSADTNIIDQHIPPTLESLTRSTLLCLSAIGIITFVTPVFVTALVPLAVAFYFIQKYFRVASKDLQDLDDSTQLPLLCHFSETAEGLTTIRAFRHEARFKQRMLELTDTNNTAYLFLSAANRWLEVRTDYLGAVIVLTAAVAAICSFYGLQSGLVGLGLTYALTVTNYLNWVVRNLADLEVQMAAVKKVNSFLTTESENYEGSIDVSQVPEDWPQHGEIKIQDLCVRYDPMLKPVLKHVNAFINPGQKVGICGRTGSGKSSLSLAFFNMVDVFEGKIIIDGIDICKLPLQTLRSRLSIILQDPVLFSGSIRFNLDPERTCSDDRLWEALEIAQLKNMVKTLPGGLDAVITEGGENFSVGQRQLFCLARAFVRKSSILIMDEATASIDMATENILQKVVMTAFADRTVVTIAHRVHTILTADLVIVMKRGNILEYGKPETLMEQEDGMFASFVKADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IJY6</accession>
    <name>A0AAR2IJY6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RALB</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2IJY6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067076.1">
        <property type="protein sequence ID" value="ENSPNAP00000038309.1"/>
        <property type="gene ID" value="ENSPNAG00000033454.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108426081"/>
    <dbReference type="CTD" id="445255"/>
    <dbReference type="GeneTree" id="ENSGT00940000155984"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04139">
        <property type="entry name" value="RalA_RalB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000203">
        <property type="entry name" value="Putative ras-related protein ral-a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000038309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="207" mass="23440" checksum="29DEC24A7ED13A02" modified="2024-11-27" version="1">MAANKGKNQSSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITEQESFTATAEFREQILRVKAEEDKIPLLLVGNKSDLEDRRQVSVDDARGKAEEWGVQYVETSAKTRANVDKVFFDLMREVRAKKMSENKDKNGKGKNKKNKKSFKERCCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2INH3</accession>
    <name>A0AAR2INH3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2INH3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066244.1">
        <property type="protein sequence ID" value="ENSPNAP00000041248.1"/>
        <property type="gene ID" value="ENSPNAG00000035057.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162556"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF107">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="15"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000041248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="141" mass="15581" checksum="2A4FC97A129D767F" modified="2024-11-27" version="1">MELKSLAACDKNQSVSLLALVLLGRRNSGKTSAGNTILGRMEFETGVQTRQSVKKHCWVDGARVAVVDTPGWSAFSSANTKHVRQEVLRSETLSSVGHWVFLLVIPVDAFSSRDCQAVEENLSILGYDVWKHTMVLFTWGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2INW3</accession>
    <name>A0AAR2INW3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">pre-mRNA 3' end processing protein WDR33</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">WD repeat-containing protein 33</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">WD repeat-containing protein of 146 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">WDR33</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the cleavage and polyadenylation specificity factor (CPSF) module of the pre-mRNA 3'-end processing complex. Interacts with CPSF3/CPSF73.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the WD repeat WDR33 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037386727.1">
        <property type="nucleotide sequence ID" value="XM_037530830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2INW3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000078225.1">
        <property type="protein sequence ID" value="ENSPNAP00000041383.1"/>
        <property type="gene ID" value="ENSPNAG00000002007.2"/>
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    <dbReference type="GeneID" id="108440863"/>
    <dbReference type="GeneTree" id="ENSGT00730000111130"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000069">
        <property type="entry name" value="WD repeat domain 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000077">
        <property type="entry name" value="WD repeat domain 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000085">
        <property type="entry name" value="WD repeat domain 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5 18" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="157"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="198"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
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            <begin position="240"/>
            <end position="266"/>
        </location>
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    <feature type="repeat" description="WD" evidence="18">
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        </location>
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        <location>
            <begin position="327"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="371"/>
            <end position="402"/>
        </location>
    </feature>
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            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="566"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="521"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="566"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="580"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="623"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="693"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="761"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="839"/>
            <end position="861"/>
        </location>
    </feature>
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        <location>
            <begin position="895"/>
            <end position="914"/>
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        <location>
            <begin position="922"/>
            <end position="935"/>
        </location>
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        <location>
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        <location>
            <begin position="1013"/>
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        <location>
            <begin position="1079"/>
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        </location>
    </feature>
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            <begin position="1093"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="1129"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022574"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1139" mass="123836" checksum="C8D19DD8AA868F36" modified="2024-11-27" version="1">MATDIGSPPRFFHMPRFQHQAPRQLFYKRPDFAQQQAMQQLTFDGKRMRKAVNRKTIDYNPSVIRHLENRLWQRDHRDLRAIQPDAGCFNDLVPPVGMLNNPMNAVTTKFVRTSTNKVKCPVFVVRWTPEGRRLVTGASSGEFTLWNGLTFNFETILQAHDSPVRAMTWSHNDMWMLTADHGGYVKYWQSNMNNVKMFQAHKEAIREASFSPTDNKFATCSDDGTVRIWDFLRCHEERILRGHGADVKCVDWHPTKGLVVSGSKDSQQPIKFWDPKTGQSLATLHAHKNTVMEVKWNLNGNWLLTASRDHLCKLFDIRNLKEELQVFRGHKKEATAVAWHPVHEGLFASGGSDGSLLFWHAGVEKEVGGMEMAHEGMIWSLAWHPLGHILCSGSNDHTSKFWTRNRPGDKMRDRYNLNLLPGMSEDGVEYDDVEPNSLAAIPGMGIPEQLKAAMEQEQTNKEAAPEAEMSIPGLDWGMDEVMQKDQKKPPTKKVPYAKPIPAQFQQAWAENKVPAMAPGEMPKERKDEKVDGKKKTQAEIEQEMAALQYTNPMLLEQLKIERMAQLQEQGVPQSASQPGAMPPFPGQGGPMPPPPQGFPQAMPPEQMPSHNMPPMGPGGMPGPFIPPGQIGPPGPPPHQGPPQGMMGHPDLHGPPRHPGPHRNMGPQAPHGMPPGPRGMQGPPQGGMPPGPGVPAGGMMGPRGQGPPQGGMMHQDMRGPAPHGNMMGPQGPMQGGMMGPPPRTHGMGNMHGMGPPPGGMHGPPNNMQGPPGNMQGPPLPYMQQGKPPHMADGNRGQFNQGQNSGPQSMMHGMGQQGPGGKDGPRGPPNHHMGPPQDRQGPGGPGGQGQGSDGGGQYWGEEGGFQENWRRGPGGPGQDYHGDPRGHRGGSGGQWENQERFSSHDGEYGGHDRYDGYEGPGEEFDQRQRRPPDDSYRRGGPSSRGSRGGYQEEYGGDESFDSQDEMSQGWGNGGGGRGRRGGPPRGGGVGRKGLLPTPDDYGPHYEGGRNQEGWEGGRDPGHDGHWDSQRSDHSMHDGQSPASRERSSSLQGMDMASLPPRKRPWHDGPGTGDMDSPSGGPDERGAGRPPREDGGYGPPSRGGRGGWGRGGPPNAGGPGGNPGPNTRRGGARGALRGGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2ITC3</accession>
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        <recommendedName>
            <fullName evidence="4">Actinodin3</fullName>
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        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <begin position="187"/>
            <end position="228"/>
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            <begin position="70"/>
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            <begin position="218"/>
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            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28201" checksum="87075165ED07A141" modified="2024-11-27" version="1">PRRAGDPKWYHKDPNFQSYYRYYSSIGHTEGLYEVDKIRMLYQQMRHLENTYGPEASKYQNTLGLRLPKSTPPPPTTKPPPPPTIPPPAPALLPHSQVDVTYLCNPKDPLCKPQIVYLPTGAVPVLCDPRYHPACKLTAPDTPVPPPAKKPIAHQPPPPIIVKGMEYDCDPYWDPDCLIDNPPRPVKVRSPVLAQPTKAPVVQKVEKEHEEDGDSPAPEDKSTLFHDPYDYRRDLYDPYLYANPADDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IUJ4</accession>
    <name>A0AAR2IUJ4_PYGNA</name>
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        <recommendedName>
            <fullName evidence="4">BtpA family membrane complex biogenesis protein</fullName>
        </recommendedName>
    </protein>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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            <taxon>Characoidei</taxon>
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        </lineage>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
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                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BtpA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_037390537.1">
        <property type="nucleotide sequence ID" value="XM_037534640.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSPNAT00000075222.1">
        <property type="protein sequence ID" value="ENSPNAP00000043345.1"/>
        <property type="gene ID" value="ENSPNAG00000032746.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108413343"/>
    <dbReference type="GeneTree" id="ENSGT00390000006020"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="CDD" id="cd04722">
        <property type="entry name" value="TIM_phosphate_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005137">
        <property type="entry name" value="BtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00259">
        <property type="entry name" value="thylakoid_BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381:SF3">
        <property type="entry name" value="SGC REGION PROTEIN SGCQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381">
        <property type="entry name" value="ZGC:162297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03437">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005956">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="268" mass="29057" checksum="E5F66A3ACDBF7BEC" modified="2024-11-27" version="1">MKFVSLFRRLKSNVIGMIHVGALPGSPLNRAPPPAIVEAACREAEEYTQAGVDGLIIENMHDVPYVFDVGPEVCACMTAVCTAVRHLCPSLPIGVQILSAANRPALATALASGLDFIRAEGFVFSHVADEGLLDACAGELLRYRRNIGAEHVQIFTDIKKKHSAHALTADVSIAETARAAEFFLSDGLIITGSATGVQADPTELKEVAKSVKLPVLIGSGVTYDNVEHYLDANALIIGSHFKKDGRWENDVDPDRVKRFMEKMHQLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IW17</accession>
    <name>A0AAR2IW17_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSPNAT00000067647.1">
        <property type="protein sequence ID" value="ENSPNAP00000043928.1"/>
        <property type="gene ID" value="ENSPNAG00000033262.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111007"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026202">
        <property type="entry name" value="GOLGB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18887">
        <property type="entry name" value="GOLGI-ASSOCIATED PROTEIN GCP360-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18887:SF4">
        <property type="entry name" value="GOLGIN SUBFAMILY B MEMBER 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="2459"/>
            <end position="2479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="32"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="192"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="382"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="486"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1550"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1655"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1750"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1883"/>
            <end position="2213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2339"/>
            <end position="2387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2480" mass="282665" checksum="C02C0C48CD360460" modified="2024-11-27" version="1">MNKSVFLSQLLKALKQKLHEKEEVLLGKAQVIDVLQGEVDSRDQQIKDLTERLRLLHSEKENVQSKMEAERHIMRTQVRDLMEKHQEELKRVAEKHESEMSEKEQVLLAQLEAVRDSSAHLAAPESSPEVRPADHSLTQRVAELEAQAKLKTDEANKSEAKFLKMKAWSKSRIRQLEDELKKAQSGSLTPDVASLRGRVNELEEEREEMLCKLEQYDELKAKNDVLETKLVVYEEQQRKLQADLEQVTKRAASQASESGSVDDAQSQVLEWQDMVTMVVEAEAVRDQAREEKNALVLRMSHIEEEREALANRQQELEEEEELVQARGLRPQKGKKPGDPAPRSLQEDFEFDRRSSFPDPRSPSGSTTPMEGENMGGGLRSVVEELELERNQLQEQILGLEERCQDLEDRLQLQARIESLQNESERLQAQLASLRAQQSRDAEKHQLLVTSLNEQLKGLSSTQECLESSLLEKEHTLAKTSEKLELIDGLKDALREKEEQHKEVSDRLFQTEHSLAEVSKNCSTFEKQCSEMKATVAELTQKLGLLKEKTQKQEATIESLQSDLDQTSDELDKLNTTHLEERAQLIHDLQSCEREIDNLKDLITEKDKEISAFFGNMAEYDAEIHELKQEVKQKEEELVRTERALTKAEQEARIINESQSSDQQALNAKIAELMEQLRVAESDLNKASQEKETNVNEINDLTRQVEEDKSTIKDLRLEMQKLSTNQRSHLSECEAQISSLKEQVLVATQKLQESEDVLSQLKETKSSNEKLKEQMLDKEQTYEKEIKSLKEERNKLLAEVMKYSSELQSLSSQLQAQVECQEQVKLGVQEKLEAISSLEQRLTKVEEEKQALNTELKIRDDTAEKLEKELSEKTERASELEGRLQSLKSESSSLQKAFEENSQELKTQKQLLLEVQENFDMVQNENSSLQTQVKSLTEENKSFKQEIEVKVQLLSQISQERDRLLSKISSLEIQHVEYEKSIEELTKEKEELNVRASELNNILEQNKQSLSEDLLGKTGECNNLRKLLEESEGTVTRLQNQIEALNTQVDELNCRIAEKEKTVGEQSAQIDAQQNGVLQLQETLTLLQEQGATLKSGLLEKDMLLQDKVSQFTSLQSELDQQKDLLSKLHGECDSLRDECTRLGRSLQENEAALSEKTLECQTHLDELSKKNESVASLSGQLEKVGELKVRLETENADMKKSLEDLLTNSSKASDEMTQRQAEIVGLHSHIQALSGQNQQIRAMYEQKEKELEGIGKVLSEMDQKVASTLEKNENLASQISTLTEQNGHLQKEKTDMIESLAEVAEEKTSLHEKISGLEMQHSENRKIIDALLKDKEGLSSRLEELSKVSEQNRQSFDESLLEKTNDCASLSKRLSESEETVKLLKGQAETSVSQFNDRLIEKEQLILDQSKQIEGYQSQLAQLQETLSLLQEQGSALKSGLMEKDAFLQQQSKECTSLQSDLDHQRELYANLQTENETLKGECLRLHQVLKEKESALEEKILECNSHTDELSRRNESVVSLSSELGAMTENIVRLESDLKRSLESHLAESAMLREELTLKQAEIVNLQDNIQALNEANNTMKAKVSKRLEEVAALQSELANRDLNVASLTQQLSTLSSNTEALQLELQQREESFRQQEAFLIQLQARSVEGEDQISQKMETIAELQREAQNLQKALQEKDLLLLKKDQEITLLSEKIAAESEASKVRMNSDMDVISKLQGELQIFLEKNDCLTGSVAEKDVLLRQEMDKYLSLKTSADNLEDTVSQLNSEVQRLSSEAAKLRTVLSEKEQVILDIGSSSSAEVTSLNETLKAKDLENENLRKQLTDLQESVSVLNTSLSAESVEVTRLKAALEESRCTMVDQNKALQDFQRKTDEAALFKDQFVESTELVSQLQGQVQELREESSRLSTAIEQKQSALINLQDKFASQAEELQETKTLLSQKSEEILNLNQAISEREEKIHAAENSISTLKQEALMLQNEIQELRVLNANISKQKDDSLASHQINADSLTVEIERLKAQHLQVAAQVNSLTENLEQRELALHAINNQYAAQVKHTEHVVSEMQKLNNLNSRLQEEYHLTKQELDKVNSERVLKEEEVSKLVLEKEELSRNHNYQIHRLQEQLQSSSMSEVVEKMKAETEQLQVQRKEVVNLRSQNTELVELKQKYDDQQRELQQAKAHKLQCERDISGYEAELAELRSERTKLQYECQALREASKVSAALTGKDMSAEQLLQLQTERAQLQSHMQHCLVEIQQRDLGFQQLNMKLQQAVEEKAAVSAQFRAVSQTLRETQLSLSELQNRCYWLENQHPQAQQDSVCVEVAPGAPQEKTSSVADLDGLDIKELRSRLAEAELQLDSAQQNVSQLSGRLEEERARREAAEEALGLAEQRVTSMEPSPSRSSQRDFSIQLETDAEWEALILDPNQHLLMRKMKSGVHSCRRWLRGRSLYCSKMLTSRAKSRYLFMAYLLTLHILVFMCFAGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2IZ25</accession>
    <name>A0AAR2IZ25_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like-conjugating enzyme ATG3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Autophagy-related protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ATG3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATG3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568399.1">
        <property type="nucleotide sequence ID" value="XM_017712910.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2IZ25"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069958.1">
        <property type="protein sequence ID" value="ENSPNAP00000043299.1"/>
        <property type="gene ID" value="ENSPNAG00000030134.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108436427"/>
    <dbReference type="CTD" id="64422"/>
    <dbReference type="GeneTree" id="ENSGT00390000010308"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.50:FF:000001">
        <property type="entry name" value="Autophagy-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007135">
        <property type="entry name" value="Atg3/Atg10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12866:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03987">
        <property type="entry name" value="Autophagy_act_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="137"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000043299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="315" mass="35588" checksum="C87FC790DD08A98A" modified="2024-11-27" version="1">MQNVINSVKGTALGVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKWASGEEAKVKPYLPKDKQFLLTRNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNSGVTGMTEAVREISLDNKDNINMNVNVGSGNGDDDDDDEGEAADMEEYEESGLLETDEATLDTSKIVDTAKCKVDAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEERQPLTVDQMYEDISQDHVKKTVTIENHPHLPPPAMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J0W1</accession>
    <name>A0AAR2J0W1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">cAMP-dependent protein kinase catalytic subunit alpha</fullName>
            <ecNumber evidence="7">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J0W1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069539.1">
        <property type="protein sequence ID" value="ENSPNAP00000045605.1"/>
        <property type="gene ID" value="ENSPNAG00000032258.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163111"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14209">
        <property type="entry name" value="STKc_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044109">
        <property type="entry name" value="STKc_PKA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF82">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17 25 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0114">cAMP</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="22" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0519">Myristate</keyword>
    <keyword evidence="15 25 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 26" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="74"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="28">
        <location>
            <begin position="329"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00020608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="381" mass="44240" checksum="AC5DA1CBA52916FA" modified="2024-11-27" version="1">MSKPSISVLDNSDGKATRRHSSGMFDNIRSIIRSFFFFSFFLHFLVKEFLAKAKEDFLKKWENPAQNTASLDQFERLKTLGTGSFGRVMLVKHKETGQHFAMKILDKQKVVKLKQIEHTLNEKRILQAVSFPFLVRLEHSFKDNTNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKGHKWFATTDWIAIYQRKVEAPFIPKCKGPGDTSNFDDYEEEEIRVSFTEKCGKEFAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J4N1</accession>
    <name>A0AAR2J4N1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">X-prolyl aminopeptidase (aminopeptidase P) 1, soluble</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">XPNPEP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J4N1"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060496.1">
        <property type="protein sequence ID" value="ENSPNAP00000045204.1"/>
        <property type="gene ID" value="ENSPNAG00000018159.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157716"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000001">
        <property type="entry name" value="Putative xaa-Pro aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000004">
        <property type="entry name" value="xaa-Pro aminopeptidase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="5">
        <location>
            <begin position="42"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="4">
        <location>
            <begin position="377"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="6">
        <location>
            <begin position="578"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000045204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="71726" checksum="18199461A41040BB" modified="2024-11-27" version="1">PLLARNPDPIQNNLRSERLLTVDVCADAAMSPKITVELLRQLRQVMKSSKYITEPIQAYIIPSGDAHQSEYIAPCDCRREFVCGFNGSAGTAIVTEQHAAMWTDGRYFLQASQQMDNNWTLMKMGLKETPSQEDWLISVLPENSKVGVDPWIIAADQWKAMAKALSGAGHSLVAVQDNLIDAVWEDRPVRPSTQLSALGLKYTGLSWQDKIRQLRGKMAERKISWFVVTALDEIACAYQKASFCVEWSDHDPPLRPLLSCQLAGIERLFLDEKRVVDPVVREHLELDSASKPELKVQCLPYESIYTELQAVSSALGPKEKVWISDKASCALTQAIPKIHRSPIPYTPLCLAKAVKNPTEIQGMRMAHIHHCHCQFCTVTEISAADKAEELRSQQKDFVGLSFPTISSVGPNGAIIHYRPLPETNRTLSLNEVYLIDSGAQYMDGTTDVTRTVHFGTPSDYEKECFTYVLKGHIAVSAAVFPNGTKGHLLDSFARAALWDSGLDYLHGTGHGVGCFLNVHEGPCGISYKTFADEPLEAGMILSDEPGYYEDGSFGIRLENVVLVVPTKPKYNYRNRGSLTFEPLTLVPIQQKMINAELLTQKERDWLNHYHRKCRETIGAELERQGRKEALDWLIQETQPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J605</accession>
    <name>A0AAR2J605_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="10">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">USP46</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with WDR48.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase C19 family. USP12/USP46 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2J605"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048099.1">
        <property type="protein sequence ID" value="ENSPNAP00000047355.1"/>
        <property type="gene ID" value="ENSPNAG00000007706.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153284"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000003">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF860">
        <property type="entry name" value="UBIQUITIN SPECIFIC PEPTIDASE 12-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="40"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="380" mass="43653" checksum="F3A37F2A3B7941C3" modified="2024-11-27" version="1">RCMLMLTKGNFHECLLQGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKVQPRRKESLLTCLADLFNSIATQKKKVGVIPPKKFISRLRKENELFDNYMQQDAHEFLNYLLNTIADLLQEEKSQERQQNGKLVQNGGGGGSGSSTGDAESDEKTQQTWVHEIFQGTLTNETRCLNCEAVSSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEQCRSKQEAQKRMRVKKLPMILALHLKRFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J834</accession>
    <name>A0AAR2J834_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transposase Tc1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2J834"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066093.1">
        <property type="protein sequence ID" value="ENSPNAP00000048105.1"/>
        <property type="gene ID" value="ENSPNAG00000031835.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286900"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055247">
        <property type="entry name" value="InsJ-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46068">
        <property type="entry name" value="PROTEIN CBG27172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46068:SF1">
        <property type="entry name" value="TRANSPOSASE IS30-LIKE HTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13518">
        <property type="entry name" value="HTH_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Insertion element IS150 protein InsJ-like helix-turn-helix" evidence="2">
        <location>
            <begin position="11"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="1">
        <location>
            <begin position="67"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="30168" checksum="4FF3ABE5C9033655" modified="2024-11-27" version="1">GSPLCEQLHEQIVQQVKNNVSQSAIAGNLGISSSTVHNIIKRFRESGEISASKRQGRKPTLNARDLRSLRRHCIKNRHHSVKDIPTWAQEHFRKPLSVNTVRRSIYKCKLKLCHAKRKPHINNTQKRRRLLWAGAHLRWTDAKWKSVLWSDESTFQIGFGNHGRCVLRAKEEKDCLDYQRKVQKPASLMVWGCVSAHGMGNLHICEGTINAERYIQVLEQHMLPSKQRLFQGHPAYFSKTMPSHILHMLQQRGFIVKECGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2J9R8</accession>
    <name>A0AAR2J9R8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2J9R8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000051263.1">
        <property type="protein sequence ID" value="ENSPNAP00000048783.1"/>
        <property type="gene ID" value="ENSPNAG00000031448.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163991"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043860053" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="623"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="667"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="712"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="747"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="779"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="553"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="822" mass="91485" checksum="AB287C11DF2E50B5" modified="2024-11-27" version="1">MIFVSMLWITGHSPTACKADPVNCRLWAEPRLPDLYIDGDFVIGGIFSMHYYMRSDMSFRELRFVRAMEFAIHEINNRTDLLPGITLGYQIHDSCAEVPMTIKAAFQLANGMEPFFNDTDSCSKSAAAAVPALVGETASTPSMSMARILGLFGIPQVIKSLTKLYNIKVPTSAALARMVKHFGWTWIGAVRSDSDYGNNGMASFLKAAQQEGICVEYSESYYRTQPRSKLERVANVIRTSTARVIVAFLASGDLRFLLEELAKQPPPPMQWIGSESWIIETELLRFNMCAGAIGFGIPRSVIPGFREFLLDLSPAQALKSPLLTEFWESSFSCSLKGSSEGARECDGSEDIRALQNPYTDTSQLRATNLVYKATYAIAHAIHGVICNDTQCDKSAKFTPWQIFQELKRVNFTTKNGYQVSFDSSGDPVAIYELINWQFMKDGSLDFVAVGHYDASRPRGQEFSMSRAISWMGRQTEVPRSVCSESCPAGTRKAVQKGKPVCCFDCIQCAEGEISNKTGYSLNCVPCPPEFWPNTQRDSCLPKPVEFLSWDDTLSIILIVFSISGAFIAVCVTAVFYKHRTSPIVRANNSELSFLLLFSLTLCFLCSLTFIGQPSEWSCMLRHIAFGITFVLCISCVLGKTIVVLMAFRATLPGSNAMKWFGPPQQRLSVLTFTLIQVLICVIWLTTSPPFPFKNLKHYKERIILECGLGSAIGFWAVLGYIGFLALVCFILAFLARKLPDNFNEAKFITFSMLIFCAVWITFIPSYVSSPGKFTVAVEVFAILASSFSLFICIFAPKCFVIVFRPEQNTKKHLMGKVPSKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JAT8</accession>
    <name>A0AAR2JAT8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Very low-density lipoprotein receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">VLDLR</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="21">Homooligomer. Binds to the extracellular matrix protein Reelin/RELN. Interacts with LRP8. Interacts with LDLRAP1. Interacts with SNX17. Interacts with DAB1. Interacts with PCSK9. Interacts with PAFAH1B3 and PAFAH1B2, the catalytic complex of (PAF-AH (I)) heterotetrameric enzyme; these interactions may modulate the Reelin pathway. Interacts with STX5; this interaction mediates VLDLR translocation from the endoplasmic reticulum to the plasma membrane. Interacts with CLU.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Clathrin-coated pit</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JAT8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064278.1">
        <property type="protein sequence ID" value="ENSPNAP00000046951.1"/>
        <property type="gene ID" value="ENSPNAG00000026791.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155460"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000052">
        <property type="entry name" value="low-density lipoprotein receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000002">
        <property type="entry name" value="low-density lipoprotein receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000025">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000038">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000043">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000046">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000049">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000051">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000053">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000057">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
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    <sequence length="962" mass="105733" checksum="AD203A08EAEF8FBE" modified="2024-11-27" version="1">MLKDVAGSRSLSTASPDSVTCAQCEPAFICKGQRAPVANLPILVFSGKCQVSCTVFSCEHNRRLWMSGPHTILMESVSSRLCRHMHICGLLEVIVQGSGSAPPLPPCTKAEVVVLLLGCCPPPHLLCIISAPFTLVISGSRTECEPSQFQCGNGRCIPSVWQCDGDEDCSDGSDESTCVRKTCAEADFVCRNGQCIPKRWHCDGEPDCEDGSDESTEVCHTRTCRMNEFSCGVGSTQCIPVFWKCDGEKDCDNGEDEINCGNITCAPLEFTCSSGRCISRNFVCNSEDDCGDGSDEQDCAPSSCGPTEFQCGNATCIPGGWVCDDDVDCQDQSDESPQRCGRNPTPPAKCSSSEIQCASGECIHRKWRCDGDADCKDGSDEKNCPARACRPDQFKCDDGSCIHGSRQCNGIRDCADGTDEVNCKNLTQCNGPDKFKCRSGECIEMSKVCNKARDCPDWSDEPIKECNLNECLLNNGGCSHLCRDMVIGYECGCTPGLQLIDRKTCGDINECLNPGICSQICINLKGGYKCECHSGYQMDPTTGVCKAVGKEPCLIFTNRRDIRKLGLERREYTQIVEQLRNTVALDADFTQQRIFWADLGQKAIFSTVLDKRDDIGTYTKVIDNVQMPVGIAVDWIYKNIYWSDLGSKTIAVANFNGTKRKVLFAGDLKEPASIAVDPLSGFLYWSDWGEPAKIEKSGMNGIDRQVLVETDIQWPNGITLDLIKSRLYWVDSKLHTLCSVDFNGDNRRKVLQSQDYLAHPFALTVFEDRVFWTDGESEAIYGANKFTGSDVTMLANNLNEPQDIIVYHELIQLSGTNWCNEKILNGGCSYMCLPAPQINKHSPKYTCVCPEGQELSSDGLRCRPALYSEGNISTSIHEVKSAKGSAAAWAILPVLLLAMAATGGYLMWRNWQLKNKKSMNFDNPVYLKTTEEDLNIDISRHSASVGHTYPAISVVNTEDDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JCJ7</accession>
    <name>A0AAR2JCJ7_PYGNA</name>
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        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017547255.1">
        <property type="nucleotide sequence ID" value="XM_017691766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JCJ7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080299.1">
        <property type="protein sequence ID" value="ENSPNAP00000047561.1"/>
        <property type="gene ID" value="ENSPNAG00000037906.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108424024"/>
    <dbReference type="CTD" id="81589"/>
    <dbReference type="GeneTree" id="ENSGT00940000165067"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01385">
        <property type="entry name" value="CLAUDIN14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000047561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="210" mass="22679" checksum="AB3FDF405204142A" modified="2024-11-27" version="1">MALQVLGITLSMIGLAGTIIICALPMWKVTAFIGTNIVVAQVFWEGLWMTCVYERTGQMQCKLYDALLDLDPSLQASRGLVVTTMALACLAFIIFLLGADCTNCLSNPRAKARIVVASGITFMLSGLTTVVPVSWTADAIIRDFHNPIVHEALKREMGAAIYLGWVTAGFLFIGGAVLCTSCPAERAHHSSRYTSAKSGTQSSYAFKNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JEE0</accession>
    <name>A0AAR2JEE0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Paraneoplastic antigen Ma-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JEE0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000070392.1">
        <property type="protein sequence ID" value="ENSPNAP00000048574.1"/>
        <property type="gene ID" value="ENSPNAG00000030112.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000205106"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048271">
        <property type="entry name" value="PNMA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF51">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN MA1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20846">
        <property type="entry name" value="PNMA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="94" mass="10540" checksum="8ECAD51763935321" modified="2024-11-27" version="1">DSKTVHIMSLLRNWCKWEGLDPEHAIMVHDVSEDAEVCDIEEALHTIKALGPVRVRGRMFDTKTQRLVALCECSEKVNTHAIPMDVPSTKGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JFB8</accession>
    <name>A0AAR2JFB8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">FACT complex subunit SSRP1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the SSRP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JFB8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000080486.1">
        <property type="protein sequence ID" value="ENSPNAP00000048541.1"/>
        <property type="gene ID" value="ENSPNAG00000026211.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157117"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21994">
        <property type="entry name" value="HMG-box_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.220:FF:000001">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000098">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000119">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="15">
        <location>
            <begin position="529"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="12">
        <location>
            <begin position="529"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="457"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="465"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000048541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="693" mass="78318" checksum="8B5A36D51BADE66A" modified="2024-11-27" version="1">MGDTLEFNEIYQEVKGCWNDGRLRFSKQSVVYKSNKTGKVDSIPAPDLSVAQWRRVCLGHGIKLATSNGHIYKYDGFKDSDFEKISAFFKANYKVELAEKDMSVKGWNWGTAKFSGPLLSFDINDSPAFEIPLASVSQCATGKNEVTVEFHQNDDAEVSLMEMRFYVPPTTGDEGTDPVEVFSQNVLSRADVIQATGDAVCIFKELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMSEEEVERRFEGKLNKNMSGSLYEMVSRVMKSLVNRKITVPGNFQGHSGAQCITCSYKASSGLLYPLERGFIYVHKPPVHLRFEEISCVNFARGTTTTRSFDFEIETKQGSQYTFSSIEREEYGKLFDFVNAKKLSIKNRGLREDMKGTDGYSDSDEDEHDAYLERMKEEGKIREEGDESEGDSGEYDSNASASDSEAEEGDSEDEGKKKKVEKKPKKAPKEKKERKPRKEKKVKDAGAPKRPMSAYMLWLNSSRERIKAENPGISITEISKKAGEMWKRLDKSRKEVCHGAHLGLGFEIYLACPSSLPSARQLSSWRCVWGCVIVLENCQVGQFPKGGAHGLLQNVTVHVRTIVCLKELQLPSAGSTHAVPDHDAATTMLDCRQETVVLVLLTRAPLHMLDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JFC5</accession>
    <name>A0AAR2JFC5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disks large homolog 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2JFC5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083957.1">
        <property type="protein sequence ID" value="ENSPNAP00000050645.1"/>
        <property type="gene ID" value="ENSPNAG00000011540.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164867"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12031">
        <property type="entry name" value="SH3_DLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001402">
        <property type="entry name" value="Discs, large homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.470:FF:000001">
        <property type="entry name" value="Disks large 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000008">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000058">
        <property type="entry name" value="Disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000049">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.470">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019590">
        <property type="entry name" value="DLG1_PEST_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015143">
        <property type="entry name" value="L27_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF5">
        <property type="entry name" value="DISKS LARGE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09058">
        <property type="entry name" value="L27_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10608">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01277">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="20">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="283"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="378"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="527"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="642"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="18">
        <location>
            <begin position="775"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="124"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="492"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="718"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="742"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="965" mass="107014" checksum="C2DAA4C8B13F468B" modified="2024-11-27" version="1">MPVRKKDAQRALLLLEEYRTKLSNAEDRQLRNSIQRVIDIFQSNLFQALIDIQEFYEVTLLDDQKCGDSVRVIDMMPPVNLWDFSSIQSTTVTSDTLPSLSTSIEDSPLLNEILHVLAKAKTSPSELDQKYRHHDEDTSPQEQSSPQLTDEAPGPELVQVAEKNISQIENVHGYVAHAHISPMKVESLECIFDGPPTVGSEDSPPPTPASLYPQSPMTVQQTDAAPSSAPIIPVIPISPVPAEAAVIPPPTSQANPPPVVVNTESLDSAPYVNGTEADYEYEEITLERGNSGLGFSIAGGTDNPHIGEDPSIYITKVIPGGAAAQDGRLRVNDVILRVNEVDVRDVTHSKAVEALKEAGSLVRLYVRRRKSVSEKVMEIKLVKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKLLAVNSACLEEVTHEHAVTALKNTTDVVYLKVAKPNSVFMNDSFAPPDITNSYSQHMENHISPPNFLSQPVPPASSGRYSPTPKTTHGEDDMTREPRKVVLHRGATGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRLVSVNGVDLRSATHEQAAATLKNAGQTVTIVAHYRPEEYRRFEAKIHDLREQMMNSSISSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVVNASDDEWWQARQVTPEGEVEEMGVIPSKRRVEKKERARLKTVKFNSKSRDKGDNQDDVLSKGQKHVTSNASDSESSYRGQEDYVLSYEPVCQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVVSREQMERDIQEHKFIEAGQYNNHLYGTSVQSVREVAEKGKHCILDVSGNAIKRLQVAQLYPIAIFIKPKSVENIMEMNRRLTEEQGRKTYDRAIKLEQEFTEHFTAIVQGDTLEEIYDQVKQIIEEQSGPYIWVLSKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JG02</accession>
    <name>A0AAR2JG02_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066429.1">
        <property type="protein sequence ID" value="ENSPNAP00000050875.1"/>
        <property type="gene ID" value="ENSPNAG00000005824.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154766"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13240">
        <property type="entry name" value="PH1_Kalirin_Trio_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13241">
        <property type="entry name" value="PH2_Kalirin_Trio_p63RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11852">
        <property type="entry name" value="SH3_Kalirin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11853">
        <property type="entry name" value="SH3_Kalirin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide exchange factor DBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000152">
        <property type="entry name" value="kalirin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000038">
        <property type="entry name" value="kalirin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000040">
        <property type="entry name" value="kalirin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000368">
        <property type="entry name" value="kalirin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000034">
        <property type="entry name" value="kalirin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000003">
        <property type="entry name" value="kalirin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000024">
        <property type="entry name" value="Kalirin RhoGEF kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000023">
        <property type="entry name" value="Kalirin RhoGEF kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000032">
        <property type="entry name" value="Kalirin RhoGEF kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000040">
        <property type="entry name" value="Kalirin RhoGEF kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000008">
        <property type="entry name" value="rho guanine nucleotide exchange factor 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000015">
        <property type="entry name" value="triple functional domain protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047054">
        <property type="entry name" value="Kalirin_TRIO_PH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028570">
        <property type="entry name" value="Kalirin_TRIO_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047053">
        <property type="entry name" value="Kalirin_TRIO_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058918">
        <property type="entry name" value="KALRN/TRIO-like_spectrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF106">
        <property type="entry name" value="TRIO, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16609">
        <property type="entry name" value="SH3-RhoG_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23587">
        <property type="entry name" value="SH3_KALRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23323">
        <property type="entry name" value="Spectrin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 18" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="26">
        <location>
            <begin position="56"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="24">
        <location>
            <begin position="1294"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="23">
        <location>
            <begin position="1481"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="22">
        <location>
            <begin position="1657"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="24">
        <location>
            <begin position="1971"/>
            <end position="2147"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="22">
        <location>
            <begin position="2534"/>
            <end position="2599"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="27">
        <location>
            <begin position="2668"/>
            <end position="2758"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="2777"/>
            <end position="3031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1603"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1752"/>
            <end position="1909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1926"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="2294"/>
            <end position="2383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="2465"/>
            <end position="2535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="2604"/>
            <end position="2643"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="756"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1630"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="1796"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1808"/>
            <end position="1821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1839"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1894"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1938"/>
            <end position="1956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="2294"/>
            <end position="2307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="2308"/>
            <end position="2326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="2327"/>
            <end position="2348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="2519"/>
            <end position="2535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="2626"/>
            <end position="2643"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="2806"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000050875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="3079" mass="347637" checksum="5DFF2D2F05F4D105" modified="2024-11-27" version="1">MNLHCGAVRDVANLSSRWQGSMLSLVDQHRWQYAQTGTTLLQLPGFRRNEEMKAMEVLPILKEKVAFLSGGRDKRGGPVLTFPSRTNHDRIRQEDLRRLIAYLAGIPSEDVCKHGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETTMVSLEGLSKVVDPSQLTPDFEGSLDYNHEEWIEIRVAFEDFTGNARHLLARLEEMQDTVTRKDFPQDLEGARRMIEEHAALKKKVMKAPVEEVDSEGQRLLQRIQSSESYANRTVPVGPGQRDGQSQPNADTQGLLPRISALLEQLHSTRQNLHQAWHVRKLQLDQCFQLRLFEQDAEKMFDWIMHNKGLFLAGYTEIGNNHSHAMELQTQHNHFAMNCMNVYVNINRIMSVGNRLLEAGHYASQQIKQISGQLEQEWKAFAAALDERSTLLEMSATFHQKCDQYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGLYEHITTAYSEVSQDGKALLDKLQRPLTPGSADSLTASANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKLRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRVQDFVRRVEQRKVLLDMSVAFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKAPHNSSINHIESVLQQLDEAQAQMEELFQERKIKLELFLQLRIFERDAIDIISDLDSWNEELSQQMSDFDTEDLTLAEQRLQHHADKALTMNNLTFDVIHQGQELLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDVAAEQHRRHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAETLLQANHYDMDMIRDCAENVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNCETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYMHRNSVNMPGMLSHVKAPEQQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYLSTHTSTGSSIHHTQELLKEHEDFHITAKQTKERVKLLIQLADGFCDKGHAHAAEIKKWVTAVDKRYRDFSLRMDKYRTSLEKALGISSDSNKASKELQLDIIPASAPGSEVKLRDAAHELNEEKRKSARRKDFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKEHIIFGNMQDLYEFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVNYCKNKPDSTQLILEHAGSYFDEIQQRHRLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKKANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSNGRSKYIYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTVHLKGALKEPIHIPKPSTTKHKGRRDGEDLDSQGDGSSQPDTISLASRTSQNTLDSDKLSGGCELTVVIHDFMGGNSNELTVRRGQTVEVLERLHDKPDWCLVRTTDRSPAQEGLVPCAMLCIAHSRSSMEMEGIFNHKDTLSVCSNDAILPGSSATLQPGHVMGSQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSRDVRKSADVGSQKDSDDSAATPQDETIEERVRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADSVPLPPPMAIQQHSLLQPDPQDDKTSSRISVRPSSSETPSAAELVSAIEELVKSKMALEDRPSSLSVEQGDSSSPSFNPSDNSLLSSSSPVDEMEERKTGFLKRRHYVLLELVETERDYVRDLGLVVEGYMTRMKEDGVPDDMKGKDKIVFGNVHQIYDWHRDFFLGELEKCLEDPDRLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDLKQRLGHRLQITDLLIKPVQRIMKYQLLLKDFLKYSKKAGLDSLDLEKAVEVMCVVPKRCNDMMNVGRLQGFDGKIVAQGRLLLQDTFMVLDSDGSLLGRMKERRVFLFEQIVIFSEPLDKKRGFSMPGFLYKNSIKVSCLGLEDSADGDPCKFTLTSRMSNGSIEAFILHSSHPGVRQVWTLQISQILESQRNFLNALTSPIEYQRNHVGGSASAPGGPAVSGSGGQGGGQSIASGVGGVVPAGSGSRSRPSRIPQPSRLPQPLRHHSPALGPGACDPDGPDKLSGRPNYSLSSCEHPSPVWRAQSHSTRISMQNCSPTKFYIPRAAVPPLALVKPRPSTISPMATPMATPAFKDCLPPCSPGPKSGGCITPSSAASFWSSMPGSPASRPDSFTFPGDACDTLGRQNQNQSHRHSTHSKDADRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTQDYVALKEDEINVCQGEVVQILASNQQNMFLVFRAATDQSPAAEGWIPGYVLGHTSASIPDSAEGTIKKSSSWHTSLRIRKKSEKREKEGRKEGKLENGYRKSRDGLANKVSVKLLNPNYIYDVPPEMLVPLSDVTCDKGECVTLRCKVCGRPKATITWKGPEENMLTNNGHFSIAYSETGEATLRIVGVASEDDGVYTCIATNDVGSVTTSASLRVLSNDGIRVMWKDNFESFYTEVAELGRGRFAVVKRCDQKGSKRTAAVKLVNKKLMKRDQVTQELSILKRLQHPHIVCLQDTFETPSSYALVLEMADQGRLLDYIVSWGNLTEEKVAFYLRDILEALHYLHNCRIAHLDVKPENLLVDQSSSQPMVKLTDFGDAVQLNSAHYVHPLLGSPEFAAPELVLGDPVSLTSDLWSLGVVTYVILSGASPFLDESVEETCLNICRLDFSFPEDYFQGVSQAARDFVCLLLRAEPSKRPAAAACLQETWLRPGGGRRSAECIDTSRLISFIDRRKHQNDLRPLGGIRPFLHSRLQPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JL97</accession>
    <name>A0AAR2JL97_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Si:ch73-242m19.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JL97"/>
    <dbReference type="Ensembl" id="ENSPNAT00000050405.1">
        <property type="protein sequence ID" value="ENSPNAP00000052803.1"/>
        <property type="gene ID" value="ENSPNAG00000031582.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165946"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040401">
        <property type="entry name" value="CCDC162"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33331:SF13">
        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING 162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33331">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000052803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1076" mass="122721" checksum="60D38152ACB2AAC1" modified="2024-11-27" version="1">MNTFLQYNSTEAIDKKWGRRSPNMALRKESNWIPHIQVKPKRDPWQQKQMSKLEELKCVDEFLQLHYRSCEESDVQRVTEILKQHAVFACEPESMGLLAMTSRLTAASAASNIWRSIYNIAQETSGKVSDVSEQERWKKAKKKNLSYSYSSSAQLLGLDQDLEDGSKDPVASRGAYVSLLYLRYLRIRELKRTCLSMLNYLRSVERRLTVDTAGLQVVGGVQVSSAEESGWMNAARGCSGSTGGPGSQHYVYNTPADYKVHCTEFMEFPEVENLSDFYSTEGQYIHVQDQRGLYVVYDAAMMDLKELENTLLLIASHYICRSREMSSGSLKSAETPKSWAGMDVDRVAVLLDIWTCETAFLEHKIELLNCYFEAYQHVIDLEEQFSLAQVITDVMHRRPRLDLGTEYFVQGYKQEVVCLQSHQQLIKLVLNTQIDKQRQYLERIWRGKQSRSCLLEYGFPLNYVPKQLVSVGGSSPTLKSVYLLELHPSLCLASDVYRTLEQAHVELCELHGVRSASQRTRLEHTILKQALLHWYTMATPGASYSQQLQRDLFSEEFIEDPVMVREVSYSVLRSAEEHERKQGRERQIFTVEIFSKLLELVTLRHRIVESASETEHLSQLYKSLTQEMGFDEFHLYMRPVQFEFAAKKEIPKQPPLFISTHFQDSSCVDRYCPSSLPLGIQELDENQIGKFSFRSEEAVLNLMNQSNLENLQVVLACQVTQKNALLGALKQALVCYWTENSVKSPQDQECVTLRASKGASHTRGRLADAFVSIQLEKVSPRDEMLNSFIKKKELMSSLISNPDEVAKIKRKLILDFCHKFSQVMSQCCLRGQIIALCYSLTSLLDKLPDIGQTHFVVGQANESRSEKGFQPDPRIFQPRPRQLLSADGKTLLNLWFIPHFSEVLIMFKTLEEKACCQALQHTLTIISALHDIVFYLVSLASLGNPKSSFGCSGELKLTADWGGSESIRAELWDIQQQMDSLTEPHSPEVVGRLLQLRREVLFLRFDMAVRCMIREAFLSSGNINAYQSVSDNMSHALSILSDSLKRDVSQFPLPQPLEPFSTEVGAHVHFSNIKVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JM83</accession>
    <name>A0AAR2JM83_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CHST8</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JM83"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044203.1">
        <property type="protein sequence ID" value="ENSPNAP00000051397.1"/>
        <property type="gene ID" value="ENSPNAG00000032653.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159100"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF7">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000051397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="331" mass="38520" checksum="83CBBA83948497CD" modified="2024-11-27" version="1">MLFIVYIYIQASVLPAKLQAQITKNHRKLLLKSSSLLNQQLIIAPAGQAKNSIKKLIKVQQSRRRLMREVCAKYRSSSIAKVVTHQHVSRIYVEDTHKLLYCEVPKAGCSNWKRVLMVLQGQAVSTGEIQHEQVHYGNHLKRLDSFDRQGIAHRLKTYTKVLFLREPFERLVSAFRDKFESPNSYYHPVFGKPIISKYRSNASKLALRTGEGVTFKEFVQYLLDVHRPVGMDIHWEPVVQLCSPCLIDYDFIGKFETIEEEANLLLRRIGAPANLTFPSFKDRNPNAARTSARITQEYFSQLNLTERQRTYDLYFMDYLMFNYSKPFADLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JQJ6</accession>
    <name>A0AAR2JQJ6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 51b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017548994.1">
        <property type="nucleotide sequence ID" value="XM_017693505.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017548996.1">
        <property type="nucleotide sequence ID" value="XM_017693507.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2JQJ6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000083088.1">
        <property type="protein sequence ID" value="ENSPNAP00000054305.1"/>
        <property type="gene ID" value="ENSPNAG00000031710.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108425100"/>
    <dbReference type="CTD" id="777622"/>
    <dbReference type="GeneTree" id="ENSGT00390000009278"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029265">
        <property type="entry name" value="TMEM51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16015">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16015:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15345">
        <property type="entry name" value="TMEM51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000054305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="27436" checksum="778A2392D6FE3BEA" modified="2024-11-27" version="1">MSYTGDNPPSSNTTSSSNSASQYAAAALGVGLVALGIVMVVWTVVPAGSVGNSSRPGQGLGSTSNTSVAAFVMLGVGVAMLLLALCLGVRNKRREQRQQQTNGNAQIDQSQPEERQAEHPEHYAVPSYEEVVGSEQYPIRQFTARQDSTTQLPAYEELIETPQSGLEGPESAHTQPTQTQNGTDDSNPTHTHPPGSNHRTGRPGLKLLPVKVRRIKSEKLGRKVSSSSTQVAVSNIEPLTPPPQYDDSPPELPHLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JRI4</accession>
    <name>A0AAR2JRI4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein lipase</fullName>
            <ecNumber evidence="5">3.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">LPL</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
            <location evidence="3">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 26">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.34" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2JRI4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000076458.1">
        <property type="protein sequence ID" value="ENSPNAP00000052799.1"/>
        <property type="gene ID" value="ENSPNAG00000025045.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157178"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042627">
        <property type="term" value="C:chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034185">
        <property type="term" value="F:apolipoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034372">
        <property type="term" value="P:very-low-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000031">
        <property type="entry name" value="Lipoprotein lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002330">
        <property type="entry name" value="Lipo_Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF3">
        <property type="entry name" value="LIPOPROTEIN LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00822">
        <property type="entry name" value="LIPOLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0162">Chylomicron</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0944">Nitration</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="13 28" id="KW-0732">Signal</keyword>
    <keyword evidence="22" id="KW-0850">VLDL</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043781380" description="Lipoprotein lipase" evidence="28">
        <location>
            <begin position="19"/>
            <end position="526"/>
        </location>
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        <location>
            <begin position="346"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="494"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="23">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="23">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="23">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00018617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022963"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023313"/>
        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
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            <dbReference type="RuleBase" id="RU004262"/>
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            <dbReference type="PROSITE" id="PS50095"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="526" mass="59218" checksum="A2D551701340D7E5" modified="2024-11-27" version="1">MGKESIWLLVIGFSLISCDPLSNSTQAPSSSNTTDWIEDYSNIQSKFSLRHVEFPEEDVCYLVPGEIGTVLDCNFKPDAQTFLVIHGWSVAGLFESWIHKLVSALFEREPTANVIVVDWLDRASHHYPTSAENTRLVGRDVAKLINWLEGLGYPLEKLHVLGYSLGAHVAGIAGNLSNNKVNRITGLDPAGPTFEHADTLKRLSPDDAKFVDVLHTNTRGSPDLSIGIQRTVGHVDIYPNGGTMQPGCSLQNTMKMIATYGLYNMDQLVKCSHERSVHLFIDSLVNQDQQSLAYRCSSKEAFYKGLCLSCRKNRCNKLGYNVNKVRTTRSAKMYLKTREMMPYKVFHFQIKLHLFSQKNMALENQPVKISLFGTRDQRDDIPVVVPSMITNTTISFLVTSDVDVGELLILKVQWETDSYLPSFFSTNEFNIRKMRIKSGETQAKVIFRPKDVEFEDLIQGGDAVVFVKSKEHEQSKREARSHRLKMHGSFFKEGVNESTTDVKKEESGMQSTEKPVEVTTKKATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2JVJ1</accession>
    <name>A0AAR2JVJ1_PYGNA</name>
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        <recommendedName>
            <fullName evidence="8">Nucleosome assembly protein 1-like 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="primary">NAP1L1</name>
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    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Characoidei</taxon>
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        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2 4">Belongs to the nucleosome assembly protein (NAP) family.</text>
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        <property type="project" value="InterPro"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="46520" checksum="3797E4B3E8BA863A" modified="2024-11-27" version="1">MIESCVHHCTFETGVFLDSEEVEIKLQLCLKCLSQLCSRQLTVQMMQNPQILAALQERLDGLVGSPSGYMESLPKVVKRRVNALKNLQVKCAHIEAKFYEEVHELERKYAALYQPLFDKRSDIIKAVYEPTDEECEWKQEEEEELTEMKEKAKLEEEKKDEEKEDPKGIPEFWLTVFKNVDLLSEMLQEHDEPILKHLQDIKVKFSDAGQPMSFTLEFHFEPNDFFTNTVLTKTYKMRSEPDESDPFSFDGPEIMGCTGCTIDWTKGKNVTLKTIKKKQKHKGRGTVRTVTKTVPNDSFFNFFSPPEVPESGELDEDSEAVLAADFEIGHFIRERIVPRAVLYFTGEAIEDDDDDYDEEGEEADDEEGEEEADEENDPDYEPKVQILPSQGLFQTDFNIQNAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K039</accession>
    <name>A0AAR2K039_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
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        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
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    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K039"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086565.1">
        <property type="protein sequence ID" value="ENSPNAP00000057678.1"/>
        <property type="gene ID" value="ENSPNAG00000035277.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177182"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000057678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="97" mass="9446" checksum="08F6F1A247ADCD37" modified="2025-10-08" version="2">MNSRKDKEECEHVCPPISLCWLFANPGVGCLLSSGVGCLLSSGVGCLLSSGVGCLLSSGVGCLLSSGVGCLLSSGVGCLLSSGVGCLLSSGVGCLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K1N0</accession>
    <name>A0AAR2K1N0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2K1N0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000045271.1">
        <property type="protein sequence ID" value="ENSPNAP00000056402.1"/>
        <property type="gene ID" value="ENSPNAG00000004831.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286927"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="364"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="785" mass="88876" checksum="59E8C64FD640BB2A" modified="2024-11-27" version="1">MLRQKKKSGSEERSGSPVSSCVSLKSDQSMMEPSNFQGGSDPQERATERSGSPVSSCVSLKSDQSMMEPSSFQGGSVPQESGSEERSGSPVSSCVSLKSDQSMMEPSNFQGGSAPQERATERSGSPVSSCVSLKSDQSMMEPSSFQGGSVPQESKGKTSASSESSHQPQKKRYSPPDQSCRGQPGSDSTVTGDKLQKKIKDVFDDLKKKIIVLVEHELESYMRILRKENTKYYRNTKNIRWELRHEVLNMTLYYLEEMQHKDLVEALQNALIGIIWHPLRKGMREKCLHVYEGIAKQGESTSLNNIYTELYITEGGAGQVNKEHEVRLIEKNNVTRHTNQMEQEVQITCMDMFKPLPEEDKPIRTVLTQGVAGIGKSICVQKFILDWAEGIKYQDIQLIFPLPFRELNLKKKERHSLMDIIYLFFPETKGLRLSDQNNVLFIFDGLDECQLPLAFQKNEFLNNVSTSAPLDAVLMNLIKGNLLPSALIWITTRPAATSMIPAECIDRVTEVRGFNDEQKEEYFRKRISDQHLASKIIDHIKESRCLHIMCHIPVFCWISATVLQRILEETDSEETPKTLTQMYTCFLIYQTIQGNVKYTGKNALDIPWDKEGMHSLGKLAFQHLEESNLIFYREDLDACGIDPSKISVYSGLCTQETVRFQDTIFSFVHLSIQEFLAALFAYISIRNNQKNVLDHQSESQESKKTEVIDFLKTAVDKALKSDHGHLDLFLCFLLGLSLESNEKLIRGLLTQKGSRSDCQKDIVEYINQSLRKIHLQRDQSISSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2K205</accession>
    <name>A0AAR2K205_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">BAX</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2K205"/>
    <dbReference type="Ensembl" id="ENSPNAT00000049019.1">
        <property type="protein sequence ID" value="ENSPNAP00000056469.1"/>
        <property type="gene ID" value="ENSPNAG00000031205.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000172289"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599:SF6">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="1">
        <location>
            <begin position="58"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000056469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="397" mass="45495" checksum="9BF32781AEDE0262" modified="2024-11-27" version="1">METHEWLTETAKDGTVWREEQIGRPLHHSPIECNPMDGEPTAFARREVSSRLQSFLCLISLQMLRTIQEWTVQHARQTQHGNWFMDLPELMAFIAILILRGIVRLPAVHDAWSATLGVPSISKIMARNRFQDIMRHLRFDDKDTRIERVANDRFAAVSDVWGSFVANCIASYNPGRHITIDEQLFPTKTRCCFLQYIATKPDKFGIKFWVACDLKSKYVCNIIPYLGKDSSRPMGEKLSEYVVMKLMEPFMDKGRTVTTDNFFTSLSLARRLLGRKTTLLGTVNKIHRELPDSAKKTLDREEFSTQVFSTSGATLTVYAPKRRKTVCILSSMHSMVETGDTQKKKPNTVTDYNSLQCGVDVMDQMVRKYTVRSGTRRWPVAVFYNLIDISALNAHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KEP6</accession>
    <name>A0AAR2KEP6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KEP6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086566.1">
        <property type="protein sequence ID" value="ENSPNAP00000060944.1"/>
        <property type="gene ID" value="ENSPNAG00000026669.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160877"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000003">
        <property type="entry name" value="alpha-1-antitrypsin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000001">
        <property type="entry name" value="Serine protease inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF363">
        <property type="entry name" value="SERINE (OR CYSTEINE) PROTEINASE INHIBITOR, CLADE A (ALPHA-1 ANTIPROTEINASE, ANTITRYPSIN), MEMBER 1, LIKE PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Serpin" evidence="6">
        <location>
            <begin position="121"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000060944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="435" mass="49580" checksum="9CFE728331FD99E5" modified="2024-11-27" version="1">RCPAESSLHVSSLISLNNNNKHIKYITLNLHYINTSTCSIVQIQYKCVSLCPGDKMCGKISFCLLVSLLLTVSLAAPHDHEDHSADHDKHLHHGKDEPHPSHDGDDDFSRRLAPHNADFAFSLYKKLAAHPEYQGKNIFFSPLSISMALSMLALGAKGETHSQIFSTLGYSALTPDQVNEGYEHLLHMLGHSQDTMLLEAGSAVASREGFKPVDKFLKDAQHYYQGEAFSVDFSKPEVAVQEVNKYIAKKTKDMITDVVKSLDPDTVMMLINFIYFRGKWKKPFDIKYTKKAEFHVDENTSVTVDMMNKESYYEYYRDWKNFSSIIMVPYKGNTSMMIVLPDEGKMQELEKNINKEHLKQGCISFVYIRFYYEWVEKMCSEDIYIKINNTEEQRALVISRPGCCILFPIHGRSSYSLNGQSFGSYAPTATRCFLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KIR6</accession>
    <name>A0AAR2KIR6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein bL21m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">MRPL21</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KIR6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000066888.1">
        <property type="protein sequence ID" value="ENSPNAP00000061986.1"/>
        <property type="gene ID" value="ENSPNAG00000003269.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005535"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="bL21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="bL21-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="L21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000061986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="181" mass="21152" checksum="F6E1F8DCAFEB6FAB" modified="2024-11-27" version="1">TSKSSLTESSYMKCKCSTFSPIWVLSYVPETSLSRPPWSDLPLLNKEEEQRRHQVVVQKVNTLIESREYGRLFAVVHFASRQWKVTNEDLILIENHIEAECGDRIVLEKVLLVGGEDFTLIGKPLLSRDLVRVEATVIEKTESWPMVHMRFWKRHRFQKKRIKRLFNLTNGEIPPPHLTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KLH7</accession>
    <name>A0AAR2KLH7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Beta-galactosidase</fullName>
            <ecNumber evidence="5 16">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GLB1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. May form higher multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="5 16"/>
    <dbReference type="RefSeq" id="XP_017539285.1">
        <property type="nucleotide sequence ID" value="XM_017683796.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KLH7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048756.1">
        <property type="protein sequence ID" value="ENSPNAP00000063319.1"/>
        <property type="gene ID" value="ENSPNAG00000010957.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108411970"/>
    <dbReference type="CTD" id="2720"/>
    <dbReference type="GeneTree" id="ENSGT00950000182942"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042340">
        <property type="term" value="P:keratan sulfate proteoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000021">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000115">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000017">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026283">
        <property type="entry name" value="B-gal_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048912">
        <property type="entry name" value="BetaGal1-like_ABD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21317">
        <property type="entry name" value="BetaGal_ABD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006336">
        <property type="entry name" value="B-gal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043882568" description="Beta-galactosidase" evidence="18">
        <location>
            <begin position="22"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="19">
        <location>
            <begin position="36"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase 1-like first all-beta" evidence="20">
        <location>
            <begin position="398"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase galactose-binding" evidence="21">
        <location>
            <begin position="540"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="653" mass="72898" checksum="E42EDBEDBAD6BFA4" modified="2024-11-27" version="1">MGFPRAAAGLLFLAVIPVSLATSSSNFTVDYKNNCFLKDGQPFRYISGSIHYSRIPQVYWKDRLLKMYMAGLNAIQTYIPWNFHEPIPGRYDFSGDRNLTHFLQLCKDLDLLVILRPGPYICGEWDMGGLPSWLLKKKDIVLRSSDADYIAAVDRWMGKLLPMIKPYLYQNGGPIITVQVENEYGSYFACDFDYLRHLVKLFRSHLGEEVVLFSTDGAGVGYLKCGSLQGVYATVDFGPGGNVTAAFEAQRHAEPHGPLVNSEFYTGWLDHWGSPHSVTSTAVLVKSLNEILEIDANVNLYMFIGGTNFEYWNGANTPYAPQPTSYDYDAPLTEAGDLTEKYFAIRDVIKKYRKIPEGAVPPSTPKFAYGQVTMKKLQTVSESLDILSFSGPVKSPYPLTFEDLNQAFGFMLYQITLPVDCPKPTPLSSPLNGVHDRAYVTIDGVAAGVLERDKTISINITGKIGSRLDILVENMGRVNYGKDINDFKGLVSNLTLGADTLSNWTVYSLSIDEAVKKGLFWKNSSPRTPDNAPTTTLSPPAFYAGSFNIPDDIPDLPQDTYIQFPDWRKGQVWINGFNLGRYWPSRGPQITLFVPEYLLSTRAPNNITVLELEASPCSPGPCVVEFISTPIINATTRYTKPFMREPFSKQDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KM41</accession>
    <name>A0AAR2KM41_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TUBB1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017563917.1">
        <property type="nucleotide sequence ID" value="XM_017708428.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KM41"/>
    <dbReference type="Ensembl" id="ENSPNAT00000068755.1">
        <property type="protein sequence ID" value="ENSPNAP00000063529.1"/>
        <property type="gene ID" value="ENSPNAG00000004952.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433695"/>
    <dbReference type="CTD" id="81027"/>
    <dbReference type="GeneTree" id="ENSGT00940000159809"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000006">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="469" mass="52770" checksum="51171915F94DD81E" modified="2024-11-27" version="1">MREIVHLQIGQCGNQIGSKFWEVISDEHGVNAVGAYEGDMDLQLERVNVYFNEAHGGKYVPRALLVDLEPGTMDAVRGSRIGQLFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRNEAESCDCMQGFQFVHSLGGGTGSGMGTLIINKIREEYPDRIMNSFSIMPSPKVSDTVVEPYNATLSIHQLIENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGVFRGRMSTKEVDEQMLAIQQKNSNYFVDWIPHNVKVAVCDIPPRGLKMASTFIGNNTAIQEIFRRVGEQFSMMFRRKAFLHWYTGEGMDEMEFTEAENNLNDLVSEYQQYQDATADQEWEAEEEVTEVEGPSSTVTSTRVEMATETVTETSVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KMX0</accession>
    <name>A0AAR2KMX0_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KMX0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000081078.1">
        <property type="protein sequence ID" value="ENSPNAP00000065678.1"/>
        <property type="gene ID" value="ENSPNAG00000036877.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000264939"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="362" mass="39793" checksum="9B10F20FDF07CBC9" modified="2024-11-27" version="1">LVLTLTGHSIRNTYLVLTLTGHSIRNTYLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTSFVLALTGHFIRNTYLVLTLTGHSIRNTYLVNTYLVLTLTGHSIRNTSLLLTLTGHSIRNTYLVLTLTGHSIRNTYLVLPLTGHFIRNTCLVLPLTGHFIRNTYLVLTLTGHSIRNTYLVLPLTGHFIRNTCLVLPLTGHSIRNTSLVLTLTGHSIRNTSLVLTLTGHSIRNTYLYIYICI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KMX7</accession>
    <name>A0AAR2KMX7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endothelin-like toxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EDN2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endothelin/sarafotoxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017540205.2">
        <property type="nucleotide sequence ID" value="XM_017684716.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2KMX7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000067680.1">
        <property type="protein sequence ID" value="ENSPNAP00000065655.1"/>
        <property type="gene ID" value="ENSPNAG00000000257.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108412621"/>
    <dbReference type="GeneTree" id="ENSGT00950000183053"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031708">
        <property type="term" value="F:endothelin B receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003100">
        <property type="term" value="P:regulation of systemic arterial blood pressure by endothelin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014826">
        <property type="term" value="P:vein smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020475">
        <property type="entry name" value="Endothelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019764">
        <property type="entry name" value="Endothelin_toxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001928">
        <property type="entry name" value="Endothln-like_toxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13874">
        <property type="entry name" value="ENDOTHELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13874:SF9">
        <property type="entry name" value="ENDOTHELIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00322">
        <property type="entry name" value="Endothelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00365">
        <property type="entry name" value="ENDOTHELIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00272">
        <property type="entry name" value="END"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00270">
        <property type="entry name" value="ENDOTHELIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0838">Vasoactive</keyword>
    <keyword evidence="5" id="KW-0839">Vasoconstrictor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043636069" description="Endothelin-like toxin domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Endothelin-like toxin" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Endothelin-like toxin" evidence="7">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000065655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="165" mass="18157" checksum="8118A0CFAE55D51A" modified="2024-11-27" version="1">MALSLHTVLLVSLGVWVLMEEGSGAPISQTSGSSSVAKRVRTKRCSCNNWMDKECIYFCHLDIIWVNTPSKITPYGLGSPLSRRRRSAARCQCANPSDATCSSFCYTSSMDPSLVVVSPLDREVDKAGKDLLTYFRQVAKANLIAAEHSAPPRKKPFQTNKSRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KNT8</accession>
    <name>A0AAR2KNT8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cysteine-rich protein 2-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ADA2A-containing complex subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">CRP2-binding partner</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lysine acetyltransferase 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KAT14</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. May function as a scaffold for the ATAC complex to promote ATAC complex stability. Has also weak histone acetyltransferase activity toward histone H4. Required for the normal progression through G1 and G2/M phases of the cell cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with the LIM 1 domain of CSRP2. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. In the complex, it probably interacts directly with KAT2A, MBIP and WDR5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KNT8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000046247.1">
        <property type="protein sequence ID" value="ENSPNAP00000063781.1"/>
        <property type="gene ID" value="ENSPNAG00000027352.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001146"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000013">
        <property type="entry name" value="cysteine-rich protein 2-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.980.20:FF:000004">
        <property type="entry name" value="Cysteine-rich protein 2-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF26">
        <property type="entry name" value="CYSTEINE-RICH PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="650"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="260"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="422"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="329"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000063781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="794" mass="89213" checksum="1AABA1B1F691AB0E" modified="2024-11-27" version="1">MEVIGTPEFSDLDEGGMDSGDQRGLPGQDEEAMCTSASEGLEEGEVEGETLLIVESEDQASVDLSHDQSGDSLNSDVGEDGEGSWNEDMSFYCDKCHKWIPSAQLRGEQPSYLKGDNFFKFICSDCSEDGKESFERMRLTWQQVVMLAMYNLSLEGTGRQGYFRWKEDICAFISRHWTFLLGTRKKTSTWWSTVAGCLSVGSPTFFRSGAQEFGEPGWWKLVQNRPPTLRPDGEKTSTAALKAKAVSKPCMDPTITVEGLRKRGGRNPVESAMQLKEKRSRTQEAKDIRRAQKEAAGFLDRSTSSTPVKMGNRGRRPDLQLEKGEVIDFSSLSSSDRTPLTSPSPSPSPDFSAPGTPASHSATPSLLSEADLIPDVMPPQALFHDDEELDGEGVIDPGIEYVPPPSMPLAAGALYVRKKQQRPAELIKQEPESEDEERGREVEEGGQDEGMRSRASAGSERRKEKGLEKAESGPRYTLLGLYEERMLLRRLESCPQALAVTPQAKRFRRKLLVRQAKRERGLPLLDADQAVSAALSLVGGMYGAQAGVATREAGKYRTTSQDLRILDRFQVTASSRKGFHQSNVSFWHRLMGSDVCADQGIKSPYTSRTLKPYIRRDYETKPLKMSLLAEIRAYPHRNDSSWVPEPDAPIDYCYVRPNHIPSVNSMCHDMFWPGVDLSECLQYPDFSVVVLYKKVVIGFGFMVPDVKYNEAYISFLLVHPEWRRAGIATFMIYHLIQTCMGKDVTLHVSASNPAMLLYQKFGFKTEEYILDFYDKYYPVDSKECRHAFFLRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2KSE8</accession>
    <name>A0AAR2KSE8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Cholinergic receptor, nicotinic, beta polypeptide 3b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2KSE8"/>
    <dbReference type="Ensembl" id="ENSPNAT00000073069.1">
        <property type="protein sequence ID" value="ENSPNAP00000067200.1"/>
        <property type="gene ID" value="ENSPNAG00000007860.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156892"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000025">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 3 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000005">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="44"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="254"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000067200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="54862" checksum="D187989F89A6595C" modified="2024-11-27" version="1">MTFFLQRLWVSALTLTLWFLCPLFTSLHVFVAAGEFVSLAEREDALLRDLFQGYQRWVRPTQHSNNTVKVRFGLKISQLVDVDEKNQLMTTNVWLWQEWIDYKLRWNPEDYGGITFIRVPSESIWLPDIVLYENADGRFEGSLMTKAIIKYNGAVTWTPPASYKSACTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLVLVNHQVDRSDFFDNGEWEILSATGMKGNRRDGTLSYPFITYSFILKRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKVSLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSATYHPMSPWVRSLFLQRLPHLLCMRGHTDRYHYPELEPHSPELKPRIRKAPPGPEGEGQALVNMLEKATCSVRYISRHIRKEHFIREVVQDWKFVAQVLDRIFLWVFLTVSVLGTILIFTPALKMFLRTPPPSSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L158</accession>
    <name>A0AAR2L158_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prepronociceptin a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the opioid neuropeptide precursor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017570570.2">
        <property type="nucleotide sequence ID" value="XM_017715081.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L158"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052212.1">
        <property type="protein sequence ID" value="ENSPNAP00000070255.1"/>
        <property type="gene ID" value="ENSPNAG00000033879.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108437751"/>
    <dbReference type="CTD" id="100529086"/>
    <dbReference type="GeneTree" id="ENSGT00950000183149"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031628">
        <property type="term" value="F:opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007600">
        <property type="term" value="P:sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006024">
        <property type="entry name" value="Opioid_neupept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438:SF2">
        <property type="entry name" value="PREPRONOCICEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438">
        <property type="entry name" value="PROENKEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01160">
        <property type="entry name" value="Opiods_neuropep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01028">
        <property type="entry name" value="OPIOIDPRCRSR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043826448" description="Prepronociceptin a" evidence="6">
        <location>
            <begin position="23"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="130"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26926" checksum="774A7CBB9F768D4F" modified="2024-11-27" version="1">MMKTPLWTLLLLGLCVPGRSDCQRDCLSCGQILPKDYAFDALVCLVECGKGFLGLNWEICRTAMEEGPLEPLSVEGAILKRAKEEVESVLPLDQGDGGLFYSGGLQRFDHAARALGLDDLGRESQSTQLNAVYQSQQSQSAEEAEEEPEGGRELQSDQEGAPVNLAKRFGGFLKSKYGYRKFMDPGRSLQKRYGGFIGVRKSARKWNNQKRFSEFLKQYLGMTTRASEFNSVSTDITQQSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4J0</accession>
    <name>A0AAR2L4J0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Shisa N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017558368.1">
        <property type="nucleotide sequence ID" value="XM_017702879.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L4J0"/>
    <dbReference type="Ensembl" id="ENSPNAT00000082331.1">
        <property type="protein sequence ID" value="ENSPNAP00000069296.1"/>
        <property type="gene ID" value="ENSPNAG00000036955.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430410"/>
    <dbReference type="GeneTree" id="ENSGT00940000157443"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395:SF25">
        <property type="entry name" value="ABLIM_ANCHOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395">
        <property type="entry name" value="SHISA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043557568" description="Shisa N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="8">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="132"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="136"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000069296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="263" mass="28964" checksum="82BD5F08AFAAADD4" modified="2024-11-27" version="1">MKELGVAVLLVSLAVTLLVHVSGEYCHGWRDTRAQWREGFHCPEQIDKREAVLCCGHCDLRYCCGDSRARLDQGTCADHSRGQETHINEHTAELPVYVPFLIVVCVFVVFVLLGSMVAVCFCLSQKPLPPARPSHSSVQESPPLPSEPQEILPLTASTGTSCGSSSSLYPLNNASQSQTSQLHPKLEKQQQNAPPPSFHLRPNPAFHRTMTLPTMLHPLMFPTAMLNPYIMPTQGLRPYRSVPSPCPPITSTGTIQRSSEVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L4W6</accession>
    <name>A0AAR2L4W6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HNRNPH3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017571368.1">
        <property type="nucleotide sequence ID" value="XM_017715879.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017571369.1">
        <property type="nucleotide sequence ID" value="XM_017715880.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017571370.1">
        <property type="nucleotide sequence ID" value="XM_017715881.2"/>
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    <dbReference type="AlphaFoldDB" id="A0AAR2L4W6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000064323.1">
        <property type="protein sequence ID" value="ENSPNAP00000071648.1"/>
        <property type="gene ID" value="ENSPNAG00000000466.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438202"/>
    <dbReference type="CTD" id="3189"/>
    <dbReference type="GeneTree" id="ENSGT00940000157720"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd12729">
        <property type="entry name" value="RRM1_hnRNPH_hnRNPH2_hnRNPF"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd12732">
        <property type="entry name" value="RRM2_hnRNPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000071">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein H isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000031">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein h3 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000131">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein h3 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034970">
        <property type="entry name" value="hnRNPH3_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="8"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="108"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="291"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="397" mass="43710" checksum="20CFD11595351C97" modified="2024-11-27" version="1">MSHNDEGYVVRIRGLPWSCTQEEVASFFSDCDIVGKVNGVCFTFSKEGRPSGEAFVELKTAEDFKKALAKDRKYMGHRYIEVFKSNRSEMDWVLKRCGPTDYDSCSGCMLRLRGLPFGCSKEEIVQFFAGLKIVPNGITLPMDYQGRSTGEAFVQFASKEIAEKALGKHKERIGHRYIEIFKSSRNEIRAYYEVPRRMMGQRPSPYDRPMMGRGGFFGPGPARGGAVMDQMRSGGGYSGGYSGFDNYNGFNNYCFGNGMFEERARGDRGRGGMGGHGYSTGEPGSGFHSGHFVHMRGLPFRATEADVANFFAPLNPIRVHIDVGPNGKPTGEADVEFGSHEDAVSAMSKDKNHMQHRYIELFLNSTSSGASEMGRGGGFYGNSGGMGSRGSGLRGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L8E6</accession>
    <name>A0AAR2L8E6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">BMP and activin membrane-bound inhibitor homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 10">Negatively regulates TGF-beta signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the BAMBI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557968.2">
        <property type="nucleotide sequence ID" value="XM_017702479.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L8E6"/>
    <dbReference type="Ensembl" id="ENSPNAT00000069082.1">
        <property type="protein sequence ID" value="ENSPNAP00000070646.1"/>
        <property type="gene ID" value="ENSPNAG00000030796.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108430169"/>
    <dbReference type="CTD" id="431736"/>
    <dbReference type="GeneTree" id="ENSGT00940000154101"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23576">
        <property type="entry name" value="TFP_LU_ECD_BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000018">
        <property type="entry name" value="BMP and activin membrane-bound inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009345">
        <property type="entry name" value="BAMBI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045806">
        <property type="entry name" value="BAMBI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045807">
        <property type="entry name" value="BAMBI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15505:SF1">
        <property type="entry name" value="BMP AND ACTIVIN MEMBRANE-BOUND INHIBITOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15505">
        <property type="entry name" value="RIIA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06211">
        <property type="entry name" value="BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19337">
        <property type="entry name" value="BAMBI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037456">
        <property type="entry name" value="BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043355591" description="BMP and activin membrane-bound inhibitor homolog" evidence="12">
        <location>
            <begin position="27"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor N-terminal" evidence="13">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor C-terminal" evidence="14">
        <location>
            <begin position="120"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="256" mass="28695" checksum="19EC430830059CF2" modified="2024-11-27" version="1">MERHPSLVSLWLQLELCAMAVLLTRGEIRCYCDAPHCVATGYMCKSELNACFTRILDPQSSKSPLSHGCYDPLLNSGTVCRTESTYDGPATLECCHEDMCNYRGLQELTYSRGEGLDRRGRPQAESGRHLVARVQEIPSAKEVWFRAAVIAVPIAGGLILVLLVMLAVRMLRSESRRLRQQRSEMLSRLHYSLHGQQLAKGRGTAKLDLECMVPLGGHENCCLTCDKIRQSDLSSQRLLSLVHWGRYSGRGKLEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L8H2</accession>
    <name>A0AAR2L8H2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017539975.1">
        <property type="nucleotide sequence ID" value="XM_017684486.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2L8H2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000079450.1">
        <property type="protein sequence ID" value="ENSPNAP00000070994.1"/>
        <property type="gene ID" value="ENSPNAG00000032970.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108412464"/>
    <dbReference type="CTD" id="100538217"/>
    <dbReference type="GeneTree" id="ENSGT01150000286911"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Pyrin" evidence="6">
        <location>
            <begin position="156"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000070994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="772" mass="88817" checksum="C03D255FDE853159" modified="2024-11-27" version="1">MSADGNDSDLEVERIMERPPSSYGSMRSDDREDEDEELDDRPQPTAHVSLGPLTRPSVIRLTRSDSPETGITALTQGQQSSVHKEGAFISQQGREQQMLRGTEDTFEEYGLRDLRRRAEFQMRMEVTSMEPQQPPQNLHPPPEGVGLQSGVVHITLTLSFVFKAMQQSLSKLNPAELVWFKRSLCSQYKNKFELSQLEECDVLDVVDRLLERRSKGEAVHLTIRTLQDINKRPIADELETVCKRAVVQYHLRNSHIRRYYNLYEGTCRPGQQRFVSDVYAEPPMFIGGDWDINTEHEVHRPNFPSNQGTPIRASDIFRPMEGDMRVRTVLMTGVPAIGLTVAVQKFIMDWTDEHANQDLQFVFSLPGRELHLVKDTEQNFLEMLTSFYPETKNVDFLGNEDCPILFIIDALELCRQPLNFKGNPDISDLMEKACADALLTSLIKGTLLPHARIWITCHRVAASKIPFQYVSRFIDLKGFTDAKKDEYFTKRTKDPALGVRVLEHIKKSPALYNICQLPLLCWIVAFIYERRFHSPDYTEHLPAITTFYTQYMIVQTNRKIERYVGTGLEASRWKDKDKDFLTCMGKLALKMVLEERDVFYENEVSDLGLDLDDVTNRGGISTEVRRACGYTRGEQGLSFVHYSMQEFMAAMYTYVTFRVKGKNVFEQQIKNKVAKLMKDRPVVDLYKLAVDRALASRSGHLDMFLRFLFGFVTPGTEAHLRGYLLPQYHPPPKGMDDVVKYVNKKIKENASPERCRNLGWCLIELEEGKNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2L8P9</accession>
    <name>A0AAR2L8P9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Retinoic acid receptor alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Heterodimer; with an rxr molecule. Binds DNA preferentially as a rar/rxr heterodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2L8P9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000057712.1">
        <property type="protein sequence ID" value="ENSPNAP00000071079.1"/>
        <property type="gene ID" value="ENSPNAG00000011944.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165576"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035259">
        <property type="term" value="F:nuclear glucocorticoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071376">
        <property type="term" value="P:cellular response to corticotropin-releasing hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048384">
        <property type="term" value="P:retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06964">
        <property type="entry name" value="NR_DBD_RAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06937">
        <property type="entry name" value="NR_LBD_RAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000001">
        <property type="entry name" value="Retinoic acid receptor beta isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000004">
        <property type="entry name" value="Retinoic acid receptor beta isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047159">
        <property type="entry name" value="NR_DBD_RAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047158">
        <property type="entry name" value="NR_LBD_RAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003078">
        <property type="entry name" value="Retinoic_acid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085:SF8">
        <property type="entry name" value="RETINOIC ACID RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01292">
        <property type="entry name" value="RETNOICACIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="15">
        <location>
            <begin position="82"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="16">
        <location>
            <begin position="181"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="413"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="426"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000071079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="50316" checksum="D23F399959592EB7" modified="2024-11-27" version="1">SNSDSESCSFTGMNCPHLNSFPVPHFSYFFPHVLGSLSPPALPTLPISGYSTPSPATIETQSTSSEEIVPSPPSPPPPPRVYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMVYTCHREKSCIINKVTRNRCQYCRLQKCLEVGMCKESVRNDRNKKKKEEKKTTECSESLTLSPDTEQMIDRVRKAHQDTFPSLCQLGKYTTSNSSERRVSLDVDLWDKFSELSTKCIIKTVEFAKQLPGFTTLSIADQITLLKAACLDILILRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLLCGDRQDLEEADKVDVLQEPLLEALKLYVRKRRPHKPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLESSGGGVSSRPAGAPPGSCSPSLSPSSAQSSPPTHSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LAY9</accession>
    <name>A0AAR2LAY9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ITPR-interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LAY9"/>
    <dbReference type="Ensembl" id="ENSPNAT00000048250.1">
        <property type="protein sequence ID" value="ENSPNAP00000073705.1"/>
        <property type="gene ID" value="ENSPNAG00000034189.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158532"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029325">
        <property type="entry name" value="ITPR-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043444">
        <property type="entry name" value="TESPA1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469:SF11">
        <property type="entry name" value="PROTEIN ITPRID2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17469">
        <property type="entry name" value="SPERM SPECIFIC ANTIGEN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14722">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01257">
        <property type="entry name" value="KRAP_IP3R_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ITPR-interacting" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000073705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="115" mass="12766" checksum="BBB2C01898F4303A" modified="2024-11-27" version="1">TVGGIPDGCIVSELLDLYEEDPEEILYNLGFGREEPDIASKIPSRFFNSPSSAKGIDIKVYLGAQMQRMELDNPNYALTSKLWVKRLSLLIKVCKCVCVSVCVCVCVCVRVCPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LFH4</accession>
    <name>A0AAR2LFH4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transmembrane protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TMEM43</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM43 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LFH4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000052473.1">
        <property type="protein sequence ID" value="ENSPNAP00000075353.1"/>
        <property type="gene ID" value="ENSPNAG00000021935.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009671"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012430">
        <property type="entry name" value="TMEM43_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07787">
        <property type="entry name" value="TMEM43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="44981" checksum="81B154904EDAEDDB" modified="2024-11-27" version="1">MSVPFSGTEGRDQHSRIHSRPNPGFLERLGETMGGMMVGVGLFFVSFYVLFTNEGRAIRTAASLDEGLSQVLTLRPDMYVDPQNDNRLVHLSAPLRTSQPLYDPNYKVAVQAVKLKRNVEMYQWVEYQDSREYKEGGETKTETTYTYNTEWKSEVINSRNFDQEIGHTNPSAMAVESVTVVAPDVWVGYFFLSKGLIDQINNFQTLHLGALPVTDPFLTVHEDYFYHTANPRRPEVGDVRVSFSYAGLSGEGSFPGPPQTVSVVAMQKEERLKPFKTRSGDTLEILYLEELTAEEVFAKEHKLNDMKTWALRAGGWFLMFLGISLTTRIIYTLVDWVPLLRDLVSVGLKLFALCVSCCLSLLTIAAGWLLYRPLLAGLIAALALVPILIARSRAPDKKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LGS4</accession>
    <name>A0AAR2LGS4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin polymerization-promoting protein family member 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPPP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LGS4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000086948.1">
        <property type="protein sequence ID" value="ENSPNAP00000075730.1"/>
        <property type="gene ID" value="ENSPNAG00000013951.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153875"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032273">
        <property type="term" value="P:positive regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000057">
        <property type="entry name" value="Tubulin polymerization-promoting protein family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008907">
        <property type="entry name" value="TPP/p25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932">
        <property type="entry name" value="P25 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932:SF16">
        <property type="entry name" value="TUBULIN POLYMERIZATION-PROMOTING PROTEIN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05517">
        <property type="entry name" value="p25-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000075730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="215" mass="24412" checksum="E952B36D180F6E33" modified="2024-11-27" version="1">MKPSSDYYYHYDYEPYINFIQYIIILHFPLFFLCRIAKMAESTDVDELLNAFKRFAVHGDTKASGKEMNGKNWAKLCKDCKVIDGKNITSTDVDIVFTKVKAKSSRVMTYEEFQKALEELAPKKFKGQSKEEALQSIYKLIEGKEPTNVGVTKVAKTKAVDRLTDTSKYTGSHKERFDESGKGKGKSGREEVVENTGYVAAYKNAGTYDEKTKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LJG5</accession>
    <name>A0AAR2LJG5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dehydrogenase/reductase (SDR family) member 13a, duplicate 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017552357.2">
        <property type="nucleotide sequence ID" value="XM_017696868.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAR2LJG5"/>
    <dbReference type="Ensembl" id="ENSPNAT00000053187.1">
        <property type="protein sequence ID" value="ENSPNAP00000074817.1"/>
        <property type="gene ID" value="ENSPNAG00000033695.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108427000"/>
    <dbReference type="CTD" id="492783"/>
    <dbReference type="GeneTree" id="ENSGT00940000155599"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF50">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE (SDR FAMILY) MEMBER 13A, DUPLICATE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000074817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="34961" checksum="B052768E3A5427E4" modified="2024-11-27" version="1">MSALLMILVSALTAYVLIYYNVFRGSRCRSRATLKGRTAVVTGSNTGIGKATALDLAKRGARVILACRNQEKAEAAVYDIRRESGNAAVLFMHLDLASLKSVRSFAETFLKTEPRLDLLINNAGMMGPGRTEDGFGMAFGVNHLGHFLLTLLLLDRLKASGNSRIINVSALLHRLGSVDFNTLNTHKALVTGQSSWHCFRAYCHSKLCNVLFTRELANHLEGTSVTCYSLHPGVIATDFVRNMSLWVRLLLLLPFSKLFFLDSEGGAQTTLHCALQDGLEPLSGRYFSSCGLQEVSTAGRDDALARKLWEVSERLCGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LXA2</accession>
    <name>A0AAR2LXA2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Shisa N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SHISAL2A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0AAR2LXA2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000060427.1">
        <property type="protein sequence ID" value="ENSPNAP00000081213.1"/>
        <property type="gene ID" value="ENSPNAG00000032223.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161304"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395:SF3">
        <property type="entry name" value="PROTEIN SHISA-LIKE-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395">
        <property type="entry name" value="SHISA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000081213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="159" mass="17431" checksum="A8BB36461760805B" modified="2024-11-27" version="1">FSQSSYLLSVCSGYYTPEKAFVRGFTCPKPGGDQRAAFCCGFNDIKYCCEDPNSFFPYEYGYMWWLSVGALVGLSIAAVVLLAFLITVCVLCYLFIATKPSRLDSGLPLSAPGHLSLTGLISHIPARCSAVGTSETTLHLFNFQSKQPLSARYSFFRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAR2LZ43</accession>
    <name>A0AAR2LZ43_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">AKT1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0AAR2LZ43"/>
    <dbReference type="Ensembl" id="ENSPNAT00000044957.1">
        <property type="protein sequence ID" value="ENSPNAP00000079932.1"/>
        <property type="gene ID" value="ENSPNAG00000024901.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158752"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01241">
        <property type="entry name" value="PH_PKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05571">
        <property type="entry name" value="STKc_PKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000033">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000027">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000838">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039026">
        <property type="entry name" value="PH_PKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="5"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="145"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="404"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000079932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="486" mass="56468" checksum="3D9C38A6805B0E84" modified="2024-11-27" version="1">MGEVVIVKEGWLHKRGEYIKTWRPRYFLLKSDGTFIGYKERPQDVDQLETPLNNFSVAQCQLMKTERPKPNTFIIRCLQWTTVIERTFHVETPEEREEWTKAIQAVADGLQKQEDERMEASFVPLDTDTEMSLAKPRLRVTMHDFEYLKLLGKGTFGKVILVKERATGKYYAMKILKKDVIVAKDEVAHTLTENRVLQGSKHPFLTGLKHSFQTNDRLCFVMEYANGGELFFHLSRDRVFSEERARFYGAEIVSALDYLHSEKKVVYRDLKLENLMLDKDGHIKITDFGLCKEGITEGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLSPEAKALLAGLLQKDPKQRLGGGPEDAKEIMQHKFFSGIEWKDVYEKKLTPPFKPQVTSETDTRYFDVEFTGQTITITPPGQGTDHSPLQYMDEHSEFSNYSNLHHILISRISLRYIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BRJ3</accession>
    <name>A0A3B4BRJ3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TAAR1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4BRJ3"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010632.2">
        <property type="protein sequence ID" value="ENSPNAP00000002327.2"/>
        <property type="gene ID" value="ENSPNAG00000031967.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244823"/>
    <dbReference type="OMA" id="CICPYFF"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="21"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002327.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="314" mass="35220" checksum="C0238D22A2A0356E" modified="2024-11-27" version="2">PKPANSKEQNNKRCRPVQTCGSIKWIRNCLLTSLLTQLHTPTNLLILSLAVADLLVGLFVMPVNIMQLIDSCWYLGEMACTVSLVVNFLSGSGSLCSLVLIAVDRYIAVTDPLLYSMKITVCKTVLLIIIGWSFFLLYILMCLYSNDHLLSSQIFTTCYGECVIYVKYSWVLVDLVVCFLAPCSVILVLYSIIFKVAQHQAKAVRAVMTDASNKHGAQSSNVSETKAAKTLSITVFVYLACYIPYYTGSLTDSNLTSSSMVWTVFIWLIYMTSSVNPLIYAIFYPWFRASVKYVLTCKIFVYPSSRLNLFPKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BV02</accession>
    <name>A0A3B4BV02_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec1 family domain containing 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SCFD2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017543459.2">
        <property type="nucleotide sequence ID" value="XM_017687970.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BV02"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008679.2">
        <property type="protein sequence ID" value="ENSPNAP00000003468.2"/>
        <property type="gene ID" value="ENSPNAG00000009871.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415019"/>
    <dbReference type="GeneTree" id="ENSGT00390000011192"/>
    <dbReference type="OMA" id="FHEYESL"/>
    <dbReference type="OrthoDB" id="549905at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003468.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="689" mass="75238" checksum="EE1DE943F57C512B" modified="2024-11-27" version="2">MSPTDSFASFPAQVWEKVLSKVKKAVVFMDEPCSESLHWSAGGAARLFGAGARNVKRFSSFEAGADDEPKAVFVVSTLLKGRTADVIKDIISLSRFQYCVVFTAVPHSLHLASGSSSSSSAESEGSPVFARFEEKLCEWMGDMNYTAEVAYAPVAFARLSPQLLLAPAFADLFPLLESDLQSVNEKRPEKRRFLSVADVDAAALPVELQVKMKAFASALDALLEFSSVREESFAVGPLSRLLAAELASHPQAKNRRKTAQNKASVVFIDRTLDLTGAVGHHGDNLVEKILSVLPPLPGHVTDVQVDMWELTHLRRTSEVQGVLAPGCLAQNQSATARLLWEAMLVAKQKEAVMEVRRQLVEAASKENLPIKMSLGRVTAEQLSSYVQLFRGSWGAVESHCGVLQLGLATAHTLRHPALTHWDACLAFERLLLQALGDSGLPDVLKQLLHLVKSSGSDTGVDPGSGDLSVDELLVLLVYVYSLAQETKSRSHDEDQVEKLEAELVGALTLLLTQQTTLSPLLRDITGVSSLEELTMERAHTALEHVFEILRGIGHARDHLKQLHSVYSAGDGVHQATYRPFLKQVLEEIFNPNRPECPDIEHTSGGLTDLLKTGFSMFMKVNRPHPSDHPLLFLVLVGGVTPSELRLVREVVSSHKAGTQVLVVSTRLLRPADVPHLLFNTNRLSPDIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BWQ2</accession>
    <name>A0A3B4BWQ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017543646.1">
        <property type="nucleotide sequence ID" value="XM_017688157.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BWQ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000002919"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009617.2">
        <property type="protein sequence ID" value="ENSPNAP00000002919.1"/>
        <property type="gene ID" value="ENSPNAG00000004696.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108415158"/>
    <dbReference type="CTD" id="113189"/>
    <dbReference type="GeneTree" id="ENSGT00940000162640"/>
    <dbReference type="OMA" id="SQKNMPH"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050655">
        <property type="term" value="P:dermatan sulfate proteoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF33">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000002919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="363" mass="42553" checksum="C2E0EB2323A2B959" modified="2018-12-05" version="1">MPPRKPESGLKRVSVTRSRSMLSSGRHSAVLPSVLTFAVIVASGGLLLLIEKGMLNRVDTPSPLANGRRPDFFYMKQREPAVDVESQFLQEIRNRTIRAMCGQRNTPRSVWSLSPLQRKTLLQHILVNDEHRFLYCYVPKVACSNWKRVLKVLSGALAHVDVKGRMDHRADLVFLSNFSPEEIRYRLQHYFKFTFVREPMARLLSAYRNKFGEIEEYQRKYGAEIVRRYRKGHVKDRRMVAGDDVTFTEFVRYLLDEDAERMNEHWMPMHNLCQPCAVEYDFIGSYEQLNSDAAYVLEHVGAPSHVRFPERQTWYKPVTKETLHYYLCSVPQKLLRELLQKYILDFSFFGYSLPNVTTEYCRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BX36</accession>
    <name>A0A3B4BX36_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017542823.1">
        <property type="nucleotide sequence ID" value="XM_017687334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BX36"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004188"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007438.2">
        <property type="protein sequence ID" value="ENSPNAP00000004188.1"/>
        <property type="gene ID" value="ENSPNAG00000010655.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414462"/>
    <dbReference type="CTD" id="336168"/>
    <dbReference type="GeneTree" id="ENSGT00940000154662"/>
    <dbReference type="OMA" id="LHIEICV"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03700">
        <property type="entry name" value="EF2_snRNP_like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000010">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000006">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000003">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000058">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF28">
        <property type="entry name" value="ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="17"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="861" mass="95849" checksum="88222E0F6780976C" modified="2018-12-05" version="1">MVNFTVDQIREIMDKKSNIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLYYELSENDLAFIKQCKDGCGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLETDELYQTFQRIVENVNVIISTYGEGEHGPMGNIMVDPVVGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEKKKAEVSSAERSKKVEEMMKKLWGDKYFDPSNGKFSKTATNAEGKKLPRTFCQLVLDPIFKVFDAIMNFKKEESQKLIDKLNVKLDVEDKEKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQRYRCELLYEGPGDDESAMGIKNCDPKAPLMMYVSKMVPTSDKGRFYAFGRVFSGVVATGQKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTYEQAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIVAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVSDDSDQVCLSKSPNKHNRLYMKARPLPDGLAEDVDKGDVTARQELKQRARYLAEKYEWEVAEARKIWCFGPDGTGPNILVDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRAVRFDIHDVTLHADAIHRGGGQIIPTARRVLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPIFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPYDANSKPSQVVAETRKRKGLKEGIPALDNFLDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BX90</accession>
    <name>A0A3B4BX90_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BX90"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003171"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009177.2">
        <property type="protein sequence ID" value="ENSPNAP00000003171.2"/>
        <property type="gene ID" value="ENSPNAG00000009623.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164411"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051832">
        <property type="entry name" value="mTOR-Rac_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829">
        <property type="entry name" value="DEP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829:SF7">
        <property type="entry name" value="DEP DOMAIN-CONTAINING MTOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="1">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="1">
        <location>
            <begin position="150"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003171.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="365" mass="41839" checksum="A43843A770E245B7" modified="2024-11-27" version="2">MEGISSVSISTQVMSRQHKAEAMIAGEQLRLRLHFGKLIKDRRVHLRTYPNCFVAQELVDWLTVHKEVPDRDTAVKVMQRLMDHDIIHHVCDKWPKFKDAKLLYRFRKDDGTFPFSIEEKVFMRGQSLYEHLLSSRECLLQVREEKSMHYKHCFAGFQLIDWLLQNGEAESRSEGVELSRMLLEHGIIQHVSLKHHFFDSGLLYQFCVNFRRRRRLAQLIEVERDGEVMIPMEDQADSPFILRTTPPPGRNTNFLSGNVVAQLIDLQLVHICTQFLKHLTTECKVPGWTSIYTAPGVRYNPKSVLKRDVTCEELLSPGAPYIKKVLTILGDTLGWGFVVRGKSPCYVQAVDPGSPAAAAGIKVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BXB7</accession>
    <name>A0A3B4BXB7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CECR2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="42514.ENSPNAP00000003196"/>
    <dbReference type="Ensembl" id="ENSPNAT00000009135.2">
        <property type="protein sequence ID" value="ENSPNAP00000003196.2"/>
        <property type="gene ID" value="ENSPNAG00000009599.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166757"/>
    <dbReference type="OMA" id="GHTMHPM"/>
    <dbReference type="OrthoDB" id="303107at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090537">
        <property type="term" value="C:CERF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029614">
        <property type="entry name" value="CECR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47092">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47092:SF1">
        <property type="entry name" value="CHROMATIN REMODELING REGULATOR CECR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="5">
        <location>
            <begin position="415"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="167"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="505"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="782"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1491"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="310"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="788"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="840"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="888"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="917"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="963"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="977"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1021"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1086"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1121"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1140"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1258"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1324"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1350"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1372"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1415"/>
            <end position="1430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003196.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1514" mass="168446" checksum="251FE79C72040C50" modified="2024-11-27" version="2">MSQGCRVSVEEVQSWWEVPAIAHFCSLFRAAFNLPDFEIEELEQALLKQDRDFLSDLVCCLLQGCYQRRDITPQAFRSYLEDIINYRWELEEGKPNPLKECSFEDLPVRTQVELLHRLCDYRLDAADVFDLLKGLDADSLRVEPLGQDGTGALYWYFYGTRMYKEEPVRRTSSERGGTPESKIPEKKRRGRPPKKKPGEMSPTEMYTAEENGVEAPQLVKERERGAWSLICDTEEQWTRLADSIKDKSSPQDRHLYRIITQNFLPEISSMVEHREREQQQKLLNPPVRNSQRLSERFVSREDDQLNVVSEADVKQREEDLERQILLAEQRREEERLLQEEREREELERAKAAEERARRRKQREERAWLLSQGKELPPELLHLEPHSPVRRSRRTKELYEIDDDYAALYKVLEALKAHKDAWPFMEPVDESYAPNYHEIIQTPMDLSTIERKLNEGEYIVKDEFVADVKLMFENCLEYNGEESEYTIMAESLERCFNRALLKHFPSEDGDTDEEFHVTKEERDRKEKKKGKSSRQSGPESLIRATEQVQKRKTPGSGKGGKVPQESSSVLAPPPPAFPYANGPSHPQNIHPGQPHLGNDPRAAMYHPGQQRAPGPPPSGMYGQHLMLDPRYSYPGQRPFMAGGPGEHISHYNMQGPPVNGPHLGPRYPVGSEARPVQPQHQQHTYIGPTHGPSLGPRPVALQPGGFCTPPSEGNMYPSAQHPDGQPMHTVGNSYPRPGVPMHNSYTSYGPPPGPYGAWPNHQGQLPRGPMMQEQTMGNQHPYGHGMMRPPRPAGYPPSNGPYRMPAVNSPGPVGHRPPLTPQDSRPHMGSMMDSPEMIALQQLSASSSRRNISYQPSPLTASAPESQHPSSMGKSSVDNRTVDGAAKGPEVELADKRLSEDSIGPPHPGPAERLEQPSVGQQSPTAPEWSRSPQKKTSAAVSSALLSPQKNSLEPKEESYGEASPQIPSNSSQPSFQNGLNSESQSQRSQDLSPLKIPLKSPEKLPLHPSQQMPLAAPPPFSQNGPSQLPQNISHQVLHSPPQQVPQGMSPQYPHNGHVPNVPHQQMPQGGSVRGPQSGPVHGMPQTVPQVSQLGAQQPASLTPLPPSHPVSHPLAQPSPADRGDQRPSEPTREPGGGSGNSNPDAKTAPANTNSHYKQQPFSPESKAQLSRPELPHNQTPASHAIGPASNTVAQYTGGMGGPAPSQYGQYMGRAVSHSVRLPYPNQTPPSQSMSPHLNPAQYPPYHQQGSAYQYHMGPQHPQAHPQYQQQHQYYPQHQQNSRHGFPATEWQRPQYPPRHPMMPNAYPPMAGTSVNGRQRENTMSPLGSEGSGGSLMSPSSLPDGVHCGSAENQSCSSPAKHSQMEEGSDQPESPKELLDLDSHNAAARRRASAQPQHNFPYNPQVMHPNMQQGAAPPPHAMPGGPYPSRPQRPSGHFPAQHPHPHLMEALQRPQQLPYSPGQSRMPMYRHPHGAGHFQGMMMPQRDMGPEHLFHPGQNMMNAAAPSGPGSKQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BY46</accession>
    <name>A0A3B4BY46_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568344.1">
        <property type="nucleotide sequence ID" value="XM_017712855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BY46"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004508"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006899.2">
        <property type="protein sequence ID" value="ENSPNAP00000004508.1"/>
        <property type="gene ID" value="ENSPNAG00000010941.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436398"/>
    <dbReference type="CTD" id="368619"/>
    <dbReference type="GeneTree" id="ENSGT00940000155232"/>
    <dbReference type="OMA" id="RSARNTW"/>
    <dbReference type="OrthoDB" id="9936647at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
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    <sequence length="231" mass="24956" checksum="80D32CCB3689DD5D" modified="2018-12-05" version="1">MTNMVTEIVAFLLTISGWVLISSTLPTDHWKVSSVDGTVITTATFWSNLWKTCVTDSTGVSNCKDFPSMLALDAYIQVCRGLMISAVCLGFFGAILALVGMKCTKIGGSETTKARITCLSGLHFILSGVCSLTTCSLYAHRITSEFFDPLFVAQKFELGAALFIGWAGSVLCILGGFIFCFSMSEGFSMREYSYNGATSFLPTQNKKSPGPHKDAADQSSSARQFGRNAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BY60</accession>
    <name>A0A3B4BY60_PYGNA</name>
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        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">WNK2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
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            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
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            <dbReference type="ChEBI" id="CHEBI:30013"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSPNAG00000009876.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157161"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003409.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="2588" mass="277981" checksum="3177B6EC57D4E06B" modified="2024-11-27" version="2">MASGHGESVRRRFIRTNLWFSESEDQGPESPESATVRDQPRSAGRCDAPGDEEPEPETQEPQQEQEQEQERKDDKDDDEEARGKASSAAGKAGSEEEAEAEVKAVCTSPGGRFLKFDIELGRGSFKSVYKGLDTDTWVEVAWCELQDRKLSKAERQRFKEEAEMLKGLQHPNIVRFYDFWESAVKGKKCIVLVTELMTSGTLKTYLKRFKVMKTKVLRSWCRQILKGLHFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGVKPASYSKVSVPEIKEIIGECIRHHWEERYSIKDLLNHAFFAEDTGVRVELAEEDDGKKTSIALRLWVEDQKKLKGKYKDSGAIEFTFDLEREVPDAVAQEMVESGFFLECDMKLVSKSIRDRVALIKWHRERVVTGGEGQAEVKSAKTQMQNLLKVPPSGIPKGGTPLPQAEPEEPEGEQVTLFCSVPTITSSTVTSDITVSSTVISEALGNQHSVPYQTLQESLSTSQRLLSPPAQLLAQLQQGAQPHLTPLHQEAQKQLAAQVHPGPQQQPLAQQESLQQPVAHLGAPQQPTTQLQQPSALLCQEAQPQSTIHMHHGIQQQPAVQLLQGIQPQLGVQPQPSAQLHHGIQQQPAAQLHQGAQKQPIAQLHQGAYQQSTPQIHQGAFLQSSVQLHQSLYQSSPPRHGSVPCESQDSLLSIENLIATRRGSTSFIDTLQRRAHLHTHLKPLNHLPCSYPKQAPAQRDTVREQVSPGPRSVSPVQKDTLPIRSDSSVEAELSAHGFDSYANSDLTPQTQAIPSTLGNCPAPSCHPHFCHGCLALLLTTRPGSGGSLDHSPVHLNQALVSGVNPPCLVHSVHPSEVRTPASPNHMRVSSPRNCPPSAFHIYVPSPKVQNGSSPIHQAAPSAVHITPPSPKHENHSCTEKTPVQPLLPTSSPSLKYRTASSHILIPSPVTSPGFPIRMSSSSPADLCSPLSPSSSGVSLTAPRSVGDSVDLFHLDRCLHHIINRRTSSPVLMDRTACQPGSSHGDPTAYSISVPQDNIQGKGSSPTTPHRAPGATLSVHGIKVGQVLMPTAALAQTMSAPATPAPAAPLAQEFHLYLHPAAFLSQTLSTLQPVVESSSEAQAHPQRLLQSAASMLSSVPPPQPQQSVHATPLLQPLQISTQFPSSYPVLPTGGTPVGSEAMPYSPVPYSSPYPSGGPPVPSPYYSSGSVHAPLSLAPIQTMPSIPGAGTPQTPVSAPLGLPAPIPLLGMALPPSLIPPQQQQVYSPAPLPDGTLLQPQPTQLSLPFPQIGQPPSSLPQQEPCISSPSAQDDTLAHVHADRTCVQSPPQREDLEPKFTAASCALVDRIQQIVPESPALPLQPVGTQTAAESGAVPSSTQQILDAASVFIPPAPDHSLEEPIPMPLPSYTCDSVNSDAASGKEMSDSYDGTTSGGKGDGKPRKHHRKSARTRSRQEKTSKPKLSMLNVCDTGDKMVECQLETHNHKMVTFKFDLDGDAPEEIATYMVENGFILPIEKEIFIDQLKDIVDKAEDMLNEEVEGERPAALRDTPLQGQAPGGVGDEGARGQRDGAAQPVYQQNVLHTGKRWFIICPVDEPPVVSQDASSGGATAVQTALTSTLTDGTAPGQPAESTLAQPLEGSTPSASVSIDSEGYATAPPSGDSDAHGFCSPLSLTTTDPLSLAPLSPVPPAPLAAALSASHPEDAAASTAAQLRAQSVTALRSSLTVDEPSSVKLGSLSPIHAAQQVADLACAASVAESVPCCPLVMPLALDVSAGIQQPSPLTPPLAQEAPSAREQLQSISERGQHPVVMQQPFSAVGGAKAPSLPQSPAPSQHPHAPNESDGEGRGRVGFVDSTIKTLDEKLRNLLYQEYVPMYPSGSAAETPGSGTEYIQSPPGPESATGESGTSTPSLIGEGLIRAGEQLPQIPERVDSLSALSDSAVGVTVSKRQAMPHSTSCSGSRSRFKMMPGPPEAQKKQRSWSSTASPAHPGSCFGERSAYTEPSMPVSTIGRFSVVSAEDEVTRRKHSSRYSAPPDFYLDAPPSLSKRGSLPRAHTSVPVDVHAARFMSSDSGAESSPAKMAPPTPSRLGRSERRGSDFMKRAVAFLRRSGRSSSVQSSDSPSRQGGVYMSSDNDSEMEDSDIKRELQRLREKHMKEISELQAHQRDEIELLYMRLGKAPPPGLSHTSTVPPAGRRRRASKHKLRAGKLLSPLVQQLRSVKKTSESSKAGDVAKYSEPSLSLNGSPARASVVSDSRSRSGTSTLPSSVSEPVQTQQPCSLKGSLSSDNIYAGLHGEGPGAYSRPGQGWPHYPQAAERVTYKSSKPRARFLSGPLSLSIWSTLKRLCLGKERGSRSGAPAASNQPQMPPGSTPPPHQPVVGLAQTQTNNSNNKTDLFPVELHRLVGTWVQEAQSPTRARPLSQRPRRATRTRTRSTGGMPPAITATSRLPLSWPRIDSHTSSLTADHLLGFQDGFLMHGSPFGEVLRSPLYIARWPALTAPLSRDVLTFSTVSPPWMAPLSPPEPPSSQNRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BY75</accession>
    <name>A0A3B4BY75_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Plasmalemma vesicle associated protein b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BY75"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006661.2">
        <property type="protein sequence ID" value="ENSPNAP00000004647.2"/>
        <property type="gene ID" value="ENSPNAG00000001528.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006166"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002693">
        <property type="term" value="P:positive regulation of cellular extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043114">
        <property type="term" value="P:regulation of vascular permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009538">
        <property type="entry name" value="PV-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21687">
        <property type="entry name" value="PLASMALEMMA VESICLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21687:SF5">
        <property type="entry name" value="PLASMALEMMA VESICLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06637">
        <property type="entry name" value="PV-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="474"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="165"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004647.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="60122" checksum="67903E48C5CB198E" modified="2024-11-27" version="2">MYNSGFSQAKMGLTAKNMHRPKDRSCGYYMRIILFFSSLIQSLIIVSLVLFLIYGEPQQTVEEKRILELEQSYSKLSLENVALRAKEKNLTQQLNITLNAKKLSEKDMTNLRRLANTSATAITSLNKKVVRTKKNTPRLPCALPGNDKYLEHRFRQAEELNRLLRDNFTEQMHITKHELENAQKDRIRYQLEAIELRRDKGFLEERINRYEKKCKEDFVHSLIGIPNVTREFLRRVDELFSKHISFQLSCDKQSIQLENIRANCSSLSTEVENKLQTYLDQVGSQVTVTIGANAKCMVENKRLNEDATWCMQNRTGIMEENKKVLKQTQQNHDKEVEQFLLEVTKLKGQNKLLENQKSLKEIEIKMLSDKVKDLNTSLTNCGKVGEKSVFLLHFFFFRFCFHLVGSYSLQVVFFFFSFQFPGGVSSSFPAQFAQNRFGGSSPGSNGMGLFSSTFGTGGGSSTAYGTSTYAKPPASSGGWPGMGLGTGQGMSRPDATGSNPAGASPFGAGKFTPDISLGKTNPILTWADSRVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYD1</accession>
    <name>A0A3B4BYD1_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lysine (K)-specific methyltransferase 2E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">KMT2E</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYD1"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003474"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008656.2">
        <property type="protein sequence ID" value="ENSPNAP00000003474.2"/>
        <property type="gene ID" value="ENSPNAG00000010029.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157862"/>
    <dbReference type="OMA" id="GYFFKPY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15550">
        <property type="entry name" value="PHD_MLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19182">
        <property type="entry name" value="SET_KMT2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000150">
        <property type="entry name" value="Inactive histone-lysine N-methyltransferase 2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044434">
        <property type="entry name" value="KMT2E_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462:SF2">
        <property type="entry name" value="INACTIVE HISTONE-LYSINE N-METHYLTRANSFERASE 2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="94"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="298"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="189"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="440"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="563"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="802"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="896"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1009"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1077"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1193"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="480"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="591"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="692"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="814"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="840"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="896"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="930"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1212"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1264"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1308"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1322"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1370"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1408"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1419"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1441"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1472"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1493"/>
            <end position="1510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003474.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1544" mass="169019" checksum="AA5F60D1084C089D" modified="2024-11-27" version="2">DVCFSEPESVEASPVVVEKSSYPHQIYSSSHHSHSYIGLPYADHNYGARPPPTPPASPPPSMLIRPGEGVFVSDEASRGTTLSTSEDGSYGADITRCICGFTHDDGYMICCDKCSVWQHIDCMGIDRQHIPETYLCERCQPRSLDRERAILLQTRKRENMSDGDTSATESGDEVPLELYTAFQHTPTSITLTTGRLSNKQADKKRKKSGDKEPAAPARAKKAFREGSRKSNRVKGSAPECEPMEPPSLWENKVKSWMERYEEASSNQYSEDVRLLLRVKEARDGKTLAYNTHTAAFKPPVESHVQKNKRILKAVRDLAADSLIIEYRGKFMLRQQFEANGYFFKRPYPFVLFYSKFDGLEMCVDARNFGNEARFIRRSCTPNAEVRHVIEDGMLHLYIYSLRSIPKGSEITIGFDYDYGSCKYKVDCACVRGNQDCPVLKHNLEPTENLGSGTRRRGRKDKEPMVRDDSGQNQNLTLDCDGPKGKTSNDSKQRKLSPLRVSISNNQDPELIEDLEEKTSVSNEVEMESEENIAERRKKMTREERKMEAILQAFARMEKREKRREQALERIGTKGEPLLETVKEEPGQKPAKVNRNKQRKSFSRNRTHIGQQRRRTRTASACSDLPPGSPGDALEPLAGETQDGETPTAPEADAQPTSAPEASPPHSGSPAPTCRSGQKYPKTKKHLVSEWMGVEKQERVSRTPEPVPERPLRISSDPEVLATQLNSLPGLSCSPHVYSTPKHYVRFSSPFLANRKTPPTSGSCKKVKEDTFIAKHVLFFAELPTPLKKRRLCPLDACMSETSTPYASPCATPTRAESTDTPGTPQLLNTPPRPRPDDASTEPSTPTHPNSNTLHTAPQEVRIPNKYTKLSLYQLLDPNRRPPSLLASPCVRPQVSENVPQETRQVPAGSQSPDPTLPETHDMAADEGMEAESSTETPTTSEPPASSYHPWMKSPERGGLSFSPVNSNLRDLTPSHTLEMGAFRPEAATSGSFSEGVPYYPCTEEGGAMTFTRSLSGDGTEGGGTAQNPPQKKKVSLLEYRKRQREARRSGSKVECGSPVSTVPPVDVFPIAVETAPETTAPLAPRTPQPSEDPDGPPQGEREGGEGQWTSSTSVEQARERGYHRALLLSDHRKDTGQGVYLLYVFALFHIGFPVPCLPKHKCFVILTCAVHSHSHETNLVSFFSPQFCSPGPSAVPQSSFRSSKEEECDPQSRPPAQPQPPSVQQPASKPPLSKPAPLTPSKLHCSTSAAPQGHYPTPTPLLPSPKSQPQGSPYRGQRAFLSQQPQSQPPQPQASSGSTTFPQYNPQNAPPPPPPPPPAPPTSATYFQPQPSPTTAPFAGLKTAVSTPFPPGSQPLLQPHHTLHFQSNAAPPPPPPPPPPHPQPGPTLLHVNLQAPPIQQHQLLLSSAPPPPPPPPPQGQSTQPSLTQQTNGSTLLSVKQAPPPPPLPPPPPAPSSGAPHPFQNMAGFQPTLIHQSAASNPTVPPSTYQQTVLPPPPPPPPQQTPPPQSPSNPNAPQITSGNRGPPPPSSAAFHNTGYMGTGWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4BYI0</accession>
    <name>A0A3B4BYI0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017540994.1">
        <property type="nucleotide sequence ID" value="XM_017685505.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4BYI0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000003630"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008393.2">
        <property type="protein sequence ID" value="ENSPNAP00000003630.1"/>
        <property type="gene ID" value="ENSPNAG00000010043.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413139"/>
    <dbReference type="CTD" id="565375"/>
    <dbReference type="GeneTree" id="ENSGT00940000155979"/>
    <dbReference type="OMA" id="CQELPYF"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000045">
        <property type="entry name" value="Potassium voltage-gated channel subfamily S member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF39">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY S MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="15"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000003630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="497" mass="56479" checksum="5F1316B33FB01852" modified="2018-12-05" version="1">MVYGQILHHRGPDDSFINLNVGGYKQKVARVVLQRFPHTRLGQLLCCRSEAAILELCDDYSAVEREYYFDRNPRFFCYVLNFYHTGKIHLMEELCVFSFSQELEYWGIKELHLDSCCSNKFQEQKENAGERDWNEDEPQNESFDSSMEELSALDKDLEKFEGTWCSEKRKQLWLRLENPAYSRSAKMLAIVSLSVVLISIVAMCIHSMPEFHKVDANEKPLEDPVLNIFETICIIWFTFEFIIRLAVTPCLRKFLSNTLNIIDFVSIVPFYATLAFEKVDPEESEELENVGKVVQILRLMRIFRILKLARHSVGLRSLGATLRHSYHEVGLLLLFLSVGISIFSVLIYFVEKEEDESELQTIPVGWWWATISMTTVGYGDTYPVTLAGKLIGTLCIICGLLVVALPITIIFNKFSKYYQRQKALVEADQFGFEGKGPIPGIPFFNMKDLYAQGVNSLLASLSSRSSVASGGDATDASSIQDIETVCPAGLVETGEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C152</accession>
    <name>A0A3B4C152_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GJB3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4C152"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000004561"/>
    <dbReference type="Ensembl" id="ENSPNAT00000006881.2">
        <property type="protein sequence ID" value="ENSPNAP00000004561.2"/>
        <property type="gene ID" value="ENSPNAG00000010955.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234513"/>
    <dbReference type="OMA" id="CPNACYN"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF65">
        <property type="entry name" value="GAP JUNCTION BETA-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="11">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="12">
        <location>
            <begin position="140"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000004561.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="269" mass="31757" checksum="49AE3CDE27240BB6" modified="2024-11-27" version="2">MDWKTLESLLSGVNKYSTGFGRIWLSLVFVFRVMVFVVAAERVWSDDTKEFDCDTKRPGCPNACYNYFFPIIHTRLWALQLIFVTCPSFLVVMHVWYREDRERKYRLTYGEGAKLYDNPGQKHGGLWWTYLFSLFFKTAIEIGFLYLLHLIYNNFDMPRSVVCDIEPCRQVTCYIARPTEKRVFTYFMVGASAICIILNVCEIFYLIAMRIFTNRICAHLRLNNSKMNIKCLFIPAVQRTPSATLHSHVERSGPPQRYYFERCTSLKYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C351</accession>
    <name>A0A3B4C351_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NCCRP1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017569384.1">
        <property type="nucleotide sequence ID" value="XM_017713895.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C351"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006193"/>
    <dbReference type="Ensembl" id="ENSPNAT00000003994.2">
        <property type="protein sequence ID" value="ENSPNAP00000006193.1"/>
        <property type="gene ID" value="ENSPNAG00000012248.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108437057"/>
    <dbReference type="CTD" id="342897"/>
    <dbReference type="GeneTree" id="ENSGT00940000161313"/>
    <dbReference type="OMA" id="DGDFSGW"/>
    <dbReference type="OrthoDB" id="1107553at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000012">
        <property type="entry name" value="F-box only protein 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="F-box_only"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
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        <property type="entry name" value="F-BOX ONLY PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125">
        <property type="entry name" value="F-BOX ONLY PROTEIN 6-LIKE PROTEIN"/>
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        <property type="entry name" value="FBA"/>
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    </dbReference>
    <dbReference type="SMART" id="SM01198">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51114">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBA" evidence="2">
        <location>
            <begin position="51"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="236" mass="26586" checksum="2CD503F6899BCAC9" modified="2018-12-05" version="1">MADEWKQKCDQLWQLGANGVPTPDGVDWKAVYEKKPLGRNLLKNPAPHGVSHSSAPPEREVTGIPPSPDQPPQFEPTGDFSGWSTSTEVLPYDTSGIPPGVVVCYLPQFRWFSMEQHIDLKAEGLWDELLDTFQPDIAVEDWYEESQLHASIYELNVKLLAADGQTVIKEHTCSPTENMENYSHDWKQVSHVFSGYGPGVRYICFRHKLKNQFMVEFFGTLVTDSSVVIKSTRSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C5R4</accession>
    <name>A0A3B4C5R4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560919.1">
        <property type="nucleotide sequence ID" value="XM_017705430.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C5R4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000005899"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004493.2">
        <property type="protein sequence ID" value="ENSPNAP00000005899.1"/>
        <property type="gene ID" value="ENSPNAG00000002050.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431935"/>
    <dbReference type="CTD" id="100004428"/>
    <dbReference type="GeneTree" id="ENSGT00940000159318"/>
    <dbReference type="OMA" id="KQPQLMI"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000211">
        <property type="entry name" value="vasorin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017208403" description="EGF-like domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="578"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="415"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="470"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="381"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000005899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="74826" checksum="57571F3DACE5DB48" modified="2018-12-05" version="1">MKPFLVLLQLLLLFLLLPLYAWASNCPKDCTCSNQDTILCFQRRGITIPSEVPMSTKNLYVFQNGIEALYQEDFIGLENLEMLDLSQNKLTKLLGDVFKPLSSLRNLDLSSNQIIHISEDSFAGLELLERLYLYNNAIKSIHSAAFDGLEQLLELKLQGNQLTTIPALKMPHLLLLDLRFNRIPSLGPSDLQTPKLESLKLAGLGLSTLNEEFLSILKNIHELDISNNQLSAFPPALREARELVILSLAGNPMGPLKWEDIENLTELQELDISNLSLQTLPESMTQLFPHLKRLTVAENPFNCLCTLAWFPRWLHNQEIVLGRTEETRCHFPPRNAGKVLQRLGHHDFGCPTTTTITTSTVKTTTLAPSLITSLQITTHVIPPLKPSDDPSTETDINLPPPVPASPSSSTDPDLRMSFCTSNICLNGGTCWLDNQGHVECTCPRGLSGRNCENTENTPLFEDFATATVTVVPEITAQIVTSSSILLDLHRYIEVRPFIRGVKLTYRNLSGLDKRPKQLNIPASYPEYTLRGLHPNSTYSVCASPLGEPSDIDSVCTEAQTPSHQPPSAQVEDPKLTTMLVPAMAILLLLVLVAVAVGVACYMHRKKAHLHLDCNPSQLELEGVKAGLDTGALPQKQQEIMSSPSAVQNGGLDYDVPLMQDYCTANNNINTLKPSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C760</accession>
    <name>A0A3B4C760_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="10">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">G6PD</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:15842"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4C760"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000006960"/>
    <dbReference type="Ensembl" id="ENSPNAT00000002651.2">
        <property type="protein sequence ID" value="ENSPNAP00000006960.1"/>
        <property type="gene ID" value="ENSPNAG00000002455.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063435"/>
    <dbReference type="OMA" id="TLIYDCL"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000013">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 10" id="KW-0521">NADP</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="37"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="214"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000006960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="526" mass="60555" checksum="354AAADC12A782BE" modified="2018-12-05" version="1">MSNVRNPLSRTRSEVFRQLRRELYDDDEFRQVPHVFVIMGASGDLAKKKIYPTLWWLFKDRLLPEETYFVGFARSRLTLDDIRAACLPYMKVKEEDSERLSAFFSRNSYLAGRYTDGESFTQLQCHLDSLAPGTAANRLFYLALPPTVYNDVTKNIHQHCLSDEGWNRVIVEKPFGRDLQSSEELSAHLSSLFNEEQIYRIDHYLGKEMVQNLMVLRFGNRIFGPIWNRESVACVVLTFKEPFGTHGRGGYFDDFGIIRDVMQNHLLQMLTLVAMEKPTSTDSDDVRDEKVKVLKCIAPVALSDVVLGQYAGNPEGEGEAKLGYLDDPTVPEGSCTATFATAVLYVHNERWEGVPFILRCGKALNERKAEVRLQFTDVPGDIFNSQCQRNELVVRVQPDEAIYAKMMTKKPGMYFSTEETELDLTYRSRYRDVKLPDAYERLILDVFSGSQMHFVRSDELKEAWRIFTPLLHQIEAERVQPMLYTYGSRGPKEADELLKRVGFHYGGTYKLHALSRGMLPFRSEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4C8T3</accession>
    <name>A0A3B4C8T3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TBC1 domain family, member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TBC1D2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4C8T3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008063"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000763.2">
        <property type="protein sequence ID" value="ENSPNAP00000008063.2"/>
        <property type="gene ID" value="ENSPNAG00000013753.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159937"/>
    <dbReference type="OMA" id="RWEFCNT"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01265">
        <property type="entry name" value="PH_TBC1D2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000014">
        <property type="entry name" value="Putative TBC1 domain family member 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000248">
        <property type="entry name" value="TBC1 domain family member 2A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000018">
        <property type="entry name" value="TBC1 domain family member 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="28"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="481"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="760"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="218"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="760"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008063.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="784" mass="91105" checksum="AC86A0170D0C4DDB" modified="2024-11-27" version="2">RLEEPNGSGDKGSNDAAVGLRAAEHISSTKLCGYLHKQGGPLKAWKYRWFTYEEKKCQLFYYRTAQDLNPLGKVELCNATFSYPLQGEEGTFHIQTPERIFILKAANRDAKMYWLQQLQLRRWQHRDQLSADSSLPTSENLMQNGQQLLDTCPANFLPTVMIPPGLVGEEAANLPVQTNPLNELVWLLHKALEAAQLEKRACAQFLAAEGEQERLEMLRHGERCAADLRDQLEQLRRENDSLQRSLIERDAHVAELQDSVKLLMEKNQAKQEVILKLSEQVAACIADPRRNISTECISTQTFRQLTQELDSLKDDIEAYKTQNKFLNSEIYQLTRLWQNSSEQEKSLMVKCAYLEASNCQMESRYLGVLRKLQENKELTAAQRDTVRTLIEDALQENLKDVIKFNSVREYDEYGFKLVPDYEVEEMKLLSKIQALEIRSNNLLSQDVGDRSVLARWTQYLGGRSPDDLSSSPELKVLLRWGIPREYRRRVWRWMVRTVTRSLQERHPNLFQQLCEKSRNTPHPASRQIQLDLHRTLTTNQHFCSPSSPALEQLHRVLLAFAWQNPTVGYCQGLNRLAALALLVLQNEEDAFWCLVAIVQFLMPQDYYTKTLLGSQADQRVFKEFMAEKLPRLVAHLEEHNVDVSLITFNWFMVVFVESLPSDILLKVWDAFIFEGTKVIFRYALALFKYKEDDILKIHDSVEIYQYLRFFTKTVTDSRKLTSIAFEDLNPFPMKLLRNRRTLHLQRLQAELRELEKLQKEYASESTQRKDKDLDLIASEDDDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CD65</accession>
    <name>A0A3B4CD65_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017569243.1">
        <property type="nucleotide sequence ID" value="XM_017713754.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CD65"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008394"/>
    <dbReference type="Ensembl" id="ENSPNAT00000000211.2">
        <property type="protein sequence ID" value="ENSPNAP00000008394.1"/>
        <property type="gene ID" value="ENSPNAG00000003005.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436970"/>
    <dbReference type="CTD" id="794171"/>
    <dbReference type="GeneTree" id="ENSGT00390000012364"/>
    <dbReference type="OMA" id="VSQCAVN"/>
    <dbReference type="OrthoDB" id="166746at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21740">
        <property type="entry name" value="C2_II_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21551">
        <property type="entry name" value="VEFS-box_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21750">
        <property type="entry name" value="ZnB-Zn_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019135">
        <property type="entry name" value="Polycomb_protein_VEFS-Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057540">
        <property type="entry name" value="Znf_SUZ12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF0">
        <property type="entry name" value="POLYCOMB PROTEIN SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09733">
        <property type="entry name" value="VEFS-Box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23320">
        <property type="entry name" value="Zn_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Polycomb protein SUZ12-like zinc finger" evidence="10">
        <location>
            <begin position="390"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Polycomb protein VEFS-Box" evidence="9">
        <location>
            <begin position="516"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="325"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="652"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="652"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="675" mass="76992" checksum="AD2C94567E308BA6" modified="2018-12-05" version="1">MAPQKHGSDHHGAGCRSGSGKAAGNHTNSGLMNAAKRPKLEQIQADHELFLQAFEKPTQIYRFLRTRNLMAPIFLHRTLTYMSHRNSRTNGKRKCFNVDDLLFKVERTKMEQESHNLASHLQLTFTGFFHKIDKNSQNSENEENSVSLEVLLIKVCHKKRKDVSCPIKQVPTGKKQVPLNPDSSQMKVGSCPTIVVSSQEFEASNSHMVKSYSLLFRVTCPGGREINGLSSRETNVIEEVPSRKKRYSSHRDDGETTFVAQMTVFDKNRRLQLLDGEYEVSMQEMEECPIGKKRATWETILDGKWVPPFDTFSHGPTLQFTLRWTGDTSERTTAPVARPLATRNSDTSGGESHRPSAPKPPQTVAVKDPVSTDLPVRREQVHVEPRQKLRVYYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLSHSRFIFNYVPHPKGARIDVSVNECYDGSYVGNPQDIHCQPGFAFSRNGPVKRTSVTHILVCRPKRPKPSLSEFLESEEGQLEQQRTYVSGHNRLYFHSDSCMPLRPQEMDVDSEDERDPEWLREKTTMQIEEFTDVNEGEKEVMKLWNLHVMKHGFIADNQMNQACMLFVEECGSIIMEKNLCRNFLLHLVSMHDFGLVSTVTIDKAMALLRQGQQWTENNCSASNSPGQASKVAEPGKARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CD79</accession>
    <name>A0A3B4CD79_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017543167.1">
        <property type="nucleotide sequence ID" value="XM_017687678.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CD79"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009488"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015868.2">
        <property type="protein sequence ID" value="ENSPNAP00000009488.1"/>
        <property type="gene ID" value="ENSPNAG00000003337.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108414774"/>
    <dbReference type="GeneTree" id="ENSGT00990000204590"/>
    <dbReference type="OMA" id="YHYLARE"/>
    <dbReference type="OrthoDB" id="3437960at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF5">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 44 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="58"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="579"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="622" mass="68926" checksum="7E7BFA7E445B7253" modified="2018-12-05" version="1">MNAHVDAGGQAQRPGPVSGRARSDHLRMEPTSESITNFSHGFTLLRELNLQRRMGQFCDCVLQLRIHPGRLFLAHKSVLAAFSPVFASLLPRHGALVDLNFPCLTPETLDSLLDYIYTGALPPSSQEESVLSAAFHLQMEQLQQVLRWRRKALAEMTHTVQDKLNQKRPLVERQSLDGTTLAPPSYYPYGSDPPESSSPSCEVVPVICCVKTAGHNKFQLQTHCQDRSSTFEHTGTVSDADKQAHHGCPQTTHGIEKASLMDQLFEPAQSKQDKLLVVLSESSLNELPGVVTEKVGSQFSNSSNSGEFQSTLPENASQTPAVLRTISEDLPSETECSQTSNNSCPQFSRCDSNGDSRCVSEMDSIQSFNPFDSGKGEHLAEILSDTFNSNLYSTNGHILKDRPHLCSQSATETCLESQTPLCLENSRSTDMTHERRALDSNVWPLGILTTEKVAVGEQPQRTGHAESEELSKMRKDYVIAYQGQLYNQCLLERCHSKADSSDSDSDRAFPVSSVEDKDSTTVLETIDITEISLSASRPFRSPGERSLTHPFQCSMCERAFSQRGSLNRHMRSHLGVRPYSCPRCSMTFSRQYRVTEHMRVHQRCFEGPTGGHLRTTAAGGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CEM9</accession>
    <name>A0A3B4CEM9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Connexin 28.1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CEM9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000008934"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015129.2">
        <property type="protein sequence ID" value="ENSPNAP00000008934.2"/>
        <property type="gene ID" value="ENSPNAG00000003148.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286930"/>
    <dbReference type="OMA" id="AGVDKVW"/>
    <dbReference type="OrthoDB" id="9993956at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF109">
        <property type="entry name" value="CONNEXIN 28.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="7">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="8">
        <location>
            <begin position="151"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000008934.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29356" checksum="C8A0A211E19BD944" modified="2024-11-27" version="2">MGDWGFLSKLLDKVQSHSTSIGKVWLTVLLIFRIMVLGAGVDKVWGDEQSGMVCNIKTPGCKNVCYNKAFPISHTRFWVLQILFVSLPTLIYLGYVLNVIHKENKMRQKLQNQAEKRGLKLPKYTDDNGKLRYKGNLLCVYVTCILVTILFETAFIVGQYYLYGFMMKPRLECSGDPCPRHTVECFMSRPTEKTIFILFMLVVGCVSLALNIAEIFYLIGRAAVYRKHPPIIPNGHSCLLSVASFNFPLFLDKYSSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CF98</accession>
    <name>A0A3B4CF98_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein lin-28 homolog A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LIN28A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the lin-28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CF98"/>
    <dbReference type="Ensembl" id="ENSPNAT00000016738.2">
        <property type="protein sequence ID" value="ENSPNAP00000010153.2"/>
        <property type="gene ID" value="ENSPNAG00000015739.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153295"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000007">
        <property type="entry name" value="Protein lin-28 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000087">
        <property type="entry name" value="Protein lin-28 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051373">
        <property type="entry name" value="Lin-28_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054081">
        <property type="entry name" value="Lin-28A-like_Znf-CCHC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109">
        <property type="entry name" value="PROTEIN LIN-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109:SF2">
        <property type="entry name" value="PROTEIN LIN-28 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21890">
        <property type="entry name" value="Lin-28A-like_zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CSD" evidence="19">
        <location>
            <begin position="25"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="18">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010153.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="196" mass="21590" checksum="5627A010E978987B" modified="2024-11-27" version="2">MGSISHQDFQGEEEQSTEEDSQSYHGTGICKWFNVRMGFGFLSMNTREGEPLDAPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSSKGLESVRVTGPGGVQCVGSEKRPKGKSTQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSVAHMVANCPIKAQQSSSGSQGKPSPLKGEEEDQSHFPVPSETTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CFL9</accession>
    <name>A0A3B4CFL9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protection of telomeres protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">POT1-like telomere end-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">POT1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the telombin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CFL9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000010762"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017512.2">
        <property type="protein sequence ID" value="ENSPNAP00000010762.2"/>
        <property type="gene ID" value="ENSPNAG00000016527.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018285"/>
    <dbReference type="OMA" id="NHVHLAK"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000783">
        <property type="term" value="C:nuclear telomere cap complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098505">
        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04497">
        <property type="entry name" value="hPOT1_OB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20374">
        <property type="entry name" value="Pot1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000119">
        <property type="entry name" value="Protection of telomeres 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000138">
        <property type="entry name" value="Protection of telomeres 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028389">
        <property type="entry name" value="POT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048953">
        <property type="entry name" value="POT1_C_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032042">
        <property type="entry name" value="POT1PC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011564">
        <property type="entry name" value="Telomer_end-bd_POT1/Cdc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513">
        <property type="entry name" value="PROTECTION OF TELOMERES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513:SF0">
        <property type="entry name" value="PROTECTION OF TELOMERES PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02765">
        <property type="entry name" value="POT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21375">
        <property type="entry name" value="POT1_C_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16686">
        <property type="entry name" value="POT1PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00976">
        <property type="entry name" value="Telo_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomeric single stranded DNA binding POT1/Cdc13" evidence="10">
        <location>
            <begin position="156"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00084040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000010762.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="778" mass="87590" checksum="1C09F051F510B551" modified="2024-11-27" version="2">MPVYAVKDVIDLDTKAPPHLQRISILDIKLTSDCVHRSVKGKVVQKGPVVSCKQRDCLLKAVIQDPKKSSASQHASINVIFFGTLAKDFSRSVSQGDMVLLAGFTISKSPTEQTDRLHPCHLEMSGPDACLSKMLYYIYSIFLWVSRCPLLHRNIYVPLNELKPRRIVNVYGVVTFFKQPFPTKGTDYCSTLKITDQSNAKVGCTIFSERLEDHPKIFRIGDVIRLHRVKTQMFNGSMTLLTSLGWSTVTFDGMLDSPVEPRTSSRTFHFDEADKRVVEALRQWASSQSLLASEPTVPLSSVQPKMYFDLTCQLLAKACMDSRCMLLKVWDGTKCEHPLINVTVAPDALEGEPTNSQDRENLTANILVYDNHVEVARTLKPGMFLRLYNLHAVPQRAPGQPHDVPCHLGFHLHGGTSYGRGLCSIPSDNPDLLTLKRVLESQAEKLASEKEVNDGTLLEVWYTPPEAIGKGLLTFFPFVYSGLTCGHTLQQRSLAHVKRCTPPAFHHVCAQVKSYKPQNLYQCLKLFCPQCTTIKEVPDDEFIAQVFQEALRGSCQPCSEHWAVTSSVDSIQLDRAITMHVSCDMVLENTQSQLLFLQGVTLDETCVISAAHKNVVPVRSEERRMRLLDLSAPFLFCERKRYYGCKQCSQSSFVEPEVSGVEVWDEHNVAKALGVQLMQYVLLLKFELEDETGTVEALLWEDAERFFHASSADIAASQEMQDKVQTIMDRLQPPGSSMAERPWLELCLIAYTVEEDGRTQVCYQITNTETREDLWARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-11-27" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CG28</accession>
    <name>A0A3B4CG28_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glucagon receptor a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017557107.1">
        <property type="nucleotide sequence ID" value="XM_017701618.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CG28"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000009409"/>
    <dbReference type="Ensembl" id="ENSPNAT00000015766.2">
        <property type="protein sequence ID" value="ENSPNAP00000009409.1"/>
        <property type="gene ID" value="ENSPNAG00000014896.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429682"/>
    <dbReference type="CTD" id="562973"/>
    <dbReference type="GeneTree" id="ENSGT00940000165036"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016519">
        <property type="term" value="F:gastric inhibitory peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004967">
        <property type="term" value="F:glucagon receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15267">
        <property type="entry name" value="7tmB1_GCGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000133">
        <property type="entry name" value="Glucagon receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001749">
        <property type="entry name" value="GPCR_2_GIP_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF29">
        <property type="entry name" value="GLUCAGON RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01129">
        <property type="entry name" value="GIPRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043343891" description="Glucagon receptor a" evidence="13">
        <location>
            <begin position="23"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="382"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="14">
        <location>
            <begin position="38"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="131"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="486"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000009409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="520" mass="59501" checksum="ED34D3EE18818BD2" modified="2024-11-27" version="1">MSQLFILLSLLILSSCIQASSALSVKTLMARWMMYKDECLQNNSRDPPVTGLVCNRTFDKYACWPDGLPNTTVSVPCPWYLPWHRTVQHGLVYLECDADGQWVKKKNTSECEPNDPDQRDMQHYGRILSKFRAMYTVGYSLSLGALVLALGILVAFRKLHCMRNNIHMNLFASFILRASSILIKDALLERPSFNIDPGITNDMEMEWLVKNETAVGCRIAMVMMQYSIIANSYWLLVEGIYLHNLLAVTVLTEGNYFSIYLCIGWGAPLIVVLPWIIVKYLYENEECWEQNINMEYWWIIRSPILLAVLINFFIFIHIIKILVSKLRAHQMRYSDYKFRLAKSTLTLIPLLGIHQVLFSFVTDESTSNGAVSLRLTKLFIDLFFNSFQGLLVAILYCFVNKEVQSEILKRWKRWKLGKDIEEEYRHTYSNTAQVTNSMLVHASAKPRLPDIPRLSSPLSSPEEKHLLAVGCPNGLDLHLRVPLTSLPHEGATNSGPPEDPELAHRVQCYKAPQGNDESNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CI77</accession>
    <name>A0A3B4CI77_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GLI1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017560684.1">
        <property type="nucleotide sequence ID" value="XM_017705195.2"/>
    </dbReference>
    <dbReference type="STRING" id="42514.ENSPNAP00000011707"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018766.2">
        <property type="protein sequence ID" value="ENSPNAP00000011707.1"/>
        <property type="gene ID" value="ENSPNAG00000017380.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108431813"/>
    <dbReference type="CTD" id="2735"/>
    <dbReference type="GeneTree" id="ENSGT00940000160235"/>
    <dbReference type="OMA" id="QTAIMQH"/>
    <dbReference type="OrthoDB" id="3214149at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035301">
        <property type="term" value="C:Hedgehog signaling complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021560">
        <property type="term" value="P:abducens nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021742">
        <property type="term" value="P:abducens nucleus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048855">
        <property type="term" value="P:adenohypophysis morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021561">
        <property type="term" value="P:facial nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021754">
        <property type="term" value="P:facial nucleus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021508">
        <property type="term" value="P:floor plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021563">
        <property type="term" value="P:glossopharyngeal nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042694">
        <property type="term" value="P:muscle cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021532">
        <property type="term" value="P:neural tube patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042476">
        <property type="term" value="P:odontogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045880">
        <property type="term" value="P:positive regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031290">
        <property type="term" value="P:retinal ganglion cell axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021731">
        <property type="term" value="P:trigeminal motor nucleus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021559">
        <property type="term" value="P:trigeminal nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021564">
        <property type="term" value="P:vagus nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021521">
        <property type="term" value="P:ventral spinal cord interneuron specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000036">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000048">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000068">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN GLI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
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            <begin position="306"/>
            <end position="338"/>
        </location>
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            <end position="371"/>
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            <begin position="372"/>
            <end position="401"/>
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            <begin position="402"/>
            <end position="432"/>
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        <location>
            <begin position="433"/>
            <end position="463"/>
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            <begin position="74"/>
            <end position="130"/>
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            <begin position="205"/>
            <end position="247"/>
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            <end position="284"/>
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            <begin position="1090"/>
            <end position="1130"/>
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        <location>
            <begin position="92"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
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            <dbReference type="PROSITE" id="PS50157"/>
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    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011707.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1454" mass="156728" checksum="B34438AADFDE7891" modified="2018-12-05" version="1">MPVDMQPHQGLYHYNTTVHQPSKGLAPSVRSPYSEVSPVCVAPPCGQLPQSRAMYNPSLTPGACMDPYMRPPQGLPPHGMLGHRSMPPPEGSSGTPYCSQSNMMSAHHGFPLNQSASEHMGSGDGSRFSTPRSILKLSKKRALSISPLSDASVDLQTVIRTSPNSLVAFVNSRCGQNAASSYGHLSVGAMSPSMGYSPSMNYQSRPQGNMYGTPLGHPPGPVGPPTRLPPHNPRLHAPPKHGHLKTEPVLGSVMDGINVKSLEEHSEGDVASPSSTGTQDPLLGVLEGRDDLDKEEKPEPEAIYETNCHWESCSKEFDTQEQLVHHINNEHIHGEKKEFVCHWKDCSREQRPFKAQYMLVVHMRRHTGEKPHKCTFEGCNKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHITKKHRGDTGPRPPGSALTPAGQGSELLVEKEEKTREDCKLLAPENAMKSQPSPGGQSSCSSERSPLGSANNNDSGVEMNLNAAGSLEDLTALEEGVGNSATEPNGGMGSGGMGMSAQALKRLENLKIDKLKQIRRQTPPSRGAGNKLPALSVSGEMIGMCGPPPLLSNRRVMELSTPELGGGGIGGMAMPCPPNERRGSGTSSLSSAYTVSRRSSMASPYLSSRRSSEVSQVGTGGHCQSVLGNDVGGDPLSPESNRRTGLCQSASGLPGLPSLTPAQQYSLKAKYAAATGGPPPTPLPNMEQPGTPCRRGGFMTEYQGQPLPPFLHQGSARRHSSNAEYGTGIIFPHQAPGNNARRASDPVRSGGDPQSLPKVQRFNSLSNMAIMGRRNALQHCGSDANLTRHMYSPRPPSITENVMMEAMAMEPQGNPTEGREQGSIMQGGDRAYMGYQQSQHHAHPGNQLSPGHEGIGCMDPSFQSQLPGQYQGQRGDMGAGGAMGQNDMANTLLQQAEYSMSTCQLSPSGPHYPSLGQPNDGPGPWRQNTQIQNPHVGVQAGMQFQDTGMQSQQYPQGIYDLNADHNHQRVTIKPEQFHPAMGGSSACQNAKPLQQHRQPSAMELCQQGYPPQTQEQGLMGKSSNPSCDYHHGQIKAQAGQQQSQAGTFPSGGSLSLGCAGSVLTEGRRSQTPMLQVKEMMVRNYVQSQQALLWEQHQEQQAADSSLSGKPDSIEMGRQPAMMQHSPQSQQPNPNLYHNSYQGYTNQNILSPPAHSRVSSSVPVKEQMGGMQGSCYGHDMVPHPPQGRKPLSRQNSLSQQASGAYLGSPPNLSPSHSTASPRRAVRLPPVQQQQQHSENFYYSGQIHMHHNVDKPLEHQDGSCLPQQHLSGIDPSTKTASLAYPESTPMSNTLENLDLENAQIDFASIIDDPDPSSYSPVNPVVGHQPSSSQASSRLTTPQNSITLPSGLSNMAIGDMTSMLTSLAGENKYLNTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CLZ0</accession>
    <name>A0A3B4CLZ0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VWFC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CHRDL2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CLZ0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000011569"/>
    <dbReference type="Ensembl" id="ENSPNAT00000018638.2">
        <property type="protein sequence ID" value="ENSPNAP00000011569.1"/>
        <property type="gene ID" value="ENSPNAG00000017261.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166571"/>
    <dbReference type="OMA" id="HGKRYAP"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045717">
        <property type="entry name" value="CHRDL1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045716">
        <property type="entry name" value="CHRDL_1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46303:SF3">
        <property type="entry name" value="CHORDIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46303">
        <property type="entry name" value="VWFC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19548">
        <property type="entry name" value="CHRDL_1_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23334">
        <property type="entry name" value="VWC2L_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
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    </dbReference>
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        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017325033" description="VWFC domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="8">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
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            <begin position="255"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000011569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="460" mass="51657" checksum="F4A081749F0901E4" modified="2018-12-05" version="1">MSYLTHLFILAALRWLVAAETEAGQSKKVPAVFCTFKEKIYNPGDSWHPYLEPFGFMFCMRCACTESGHVKCNTIKCPVLRCENPVTDSQQCCPRCADEHRTPAGLRAPLKTCRYNGTVFQTGETFTNHDLFPSRQSNQCVMCTCSNGNIFCALKTCQPITCSSPVSVPDTCCLVCKEGPTDINSASYGDGGQQLNRGVRHSLDQCAGEQVRGRAVRATPSTLRGSLRGLNLHTLHLKGAAETTVKILLQRKHQRACVYSGKTYSHGDVWHPVLGKVLECILCTCRDGLQECRRITCPTQYPCKHPMKIEGKCCKICPEIKAESNRTECYLGHDNNSLLVYKVEPSSAAHTEDTVRTIAIERQGATEIEVQVWKTVEGVLHLMETGDVQKKDLIEHPENYVLLTTLDEDTWRKFKEEEDKERDVNRSRTCEDGIKEVWVRTETAWCSLHLLMAEILLSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CNY7</accession>
    <name>A0A3B4CNY7_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017555779.1">
        <property type="nucleotide sequence ID" value="XM_017700290.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393186.1">
        <property type="nucleotide sequence ID" value="XM_037537289.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037393187.1">
        <property type="nucleotide sequence ID" value="XM_037537290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CNY7"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013767"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037056.2">
        <property type="protein sequence ID" value="ENSPNAP00000013767.1"/>
        <property type="gene ID" value="ENSPNAG00000019638.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108428911"/>
    <dbReference type="GeneTree" id="ENSGT00940000165838"/>
    <dbReference type="OMA" id="HISGCEL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061668">
        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF28">
        <property type="entry name" value="SI:CH211-120K19.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="239" mass="26945" checksum="85D7C60D79381290" modified="2018-12-05" version="1">MNRAWAIFRSYPYMSNVVGYTTLFASADFIQQSMLGGAQGRNVQLSEEASEQQASVTHREDITVAGQSEKAHLKESESCKNTSGEKHSSVKEKSIHSIDWAQTARVALVGLCFHSNFNYHWLRALERMFPGGGTKRISVKVFLDQLIAAPVTISAFYIGLSTLERQQDPFEDWKKKFWTSYKTGIVYWSTMQAVNFSLVPPVARTVFVGGISLGWTVFLCHFRQQSNESVEPVEPPKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQD2</accession>
    <name>A0A3B4CQD2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 220</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017563591.2">
        <property type="nucleotide sequence ID" value="XM_017708102.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQD2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000036649.2">
        <property type="protein sequence ID" value="ENSPNAP00000012759.2"/>
        <property type="gene ID" value="ENSPNAG00000006421.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108433493"/>
    <dbReference type="GeneTree" id="ENSGT00940000168279"/>
    <dbReference type="OMA" id="WAPALWR"/>
    <dbReference type="OrthoDB" id="9924288at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029377">
        <property type="entry name" value="TMEM220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34262">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34262:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15071">
        <property type="entry name" value="TMEM220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000012759.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="18877" checksum="1159685BE51B33B8" modified="2024-11-27" version="2">MAKNRTTGFILPVWRLCNLLMSAFFALATYVQINDPDAGLWMVGYAVPAGLCLLITCQPQITETRPWRRTADLHVMMATGFGAMLGWTLYKESVTHIFHQEEGREFCGLMLTVLWLLLCRHSGRGGVGVLRLCTAVGITVFPFVAWIYYYINKELRADWPSHCTTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CQY9</accession>
    <name>A0A3B4CQY9_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RGS20</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017575142.1">
        <property type="nucleotide sequence ID" value="XM_017719653.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CQY9"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000013191"/>
    <dbReference type="Ensembl" id="ENSPNAT00000020708.2">
        <property type="protein sequence ID" value="ENSPNAP00000013191.1"/>
        <property type="gene ID" value="ENSPNAG00000019032.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108440656"/>
    <dbReference type="CTD" id="8601"/>
    <dbReference type="GeneTree" id="ENSGT00940000159123"/>
    <dbReference type="OrthoDB" id="10266999at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF277">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="168"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000013191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="294" mass="33388" checksum="6EFCE455DEA92C96" modified="2018-12-05" version="1">MPANRVVIQWEVSSVVQCVNHTHSFMCGRLQRLLFACRHGEGYLAPGSYESDTEHHGNGPEDPISFQPMGSERMEMRKRQLSGQQEAVAGVTVTAPAQQGQAGQGQRASNACCFCWCCCCSCSCLTVRNEDREERNRRASCDVKAEGTTDCEESPKPTVDEVRLWGQSFDKLMLCPAGRSAFRQFLRTEFSEENMLFWLACEEFSKETNKSAIEEKARLIYEDYISILSPKEVSLDSRVREVINRNMLEPTSHTFDDAQLQIYTLMQRDSYPRFINSPVYKTLLKSVSEQSPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CT50</accession>
    <name>A0A3B4CT50_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <shortName evidence="9">HIB-CoA hydrolase</shortName>
            <shortName evidence="9">HIBYL-CoA-H</shortName>
            <ecNumber evidence="9">3.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">3-hydroxyisobutyryl-coenzyme A hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HIBCH</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017569007.1">
        <property type="nucleotide sequence ID" value="XM_017713518.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CT50"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000014565"/>
    <dbReference type="Ensembl" id="ENSPNAT00000022500.2">
        <property type="protein sequence ID" value="ENSPNAP00000014565.1"/>
        <property type="gene ID" value="ENSPNAG00000020494.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436811"/>
    <dbReference type="CTD" id="26275"/>
    <dbReference type="GeneTree" id="ENSGT00890000139491"/>
    <dbReference type="OMA" id="EVFTMEY"/>
    <dbReference type="OrthoDB" id="1737613at2759"/>
    <dbReference type="UniPathway" id="UPA00362"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000026">
        <property type="entry name" value="3-hydroxyisobutyryl-CoA hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004127">
        <property type="entry name" value="PRK05617.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="10">
        <location>
            <begin position="43"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000014565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="382" mass="42107" checksum="6F217289FA64A970" modified="2018-12-05" version="1">MALSALKTALSLRSLYRLQKIQAHMMSSNTGKDVLLERVGNAGVITLNRPKILNALSLDMIRQIYPQLKKWEKDSETDIVIIKGTGDRAFCAGGDIRSLADAGKVGDPLTQDLFREEYTLNNAIGTFKKPYVALISGITMGAGFGLSVHGQFRVATEKTLFAMPETAIGLFPDVGGGYFLPRLQGKLGLFLALTGFRLRGRDVQRAGVATHFVESEKLGALEKDLVELKSPSVSDVAQVLNTYQGQSTLDAEKPFALQQHTGAIDRLFKASSVEEILENLQKDGSAFAQKQLETLAKMSPTSLKLTFRQMQMGAGMSLQEVFVMEYRLSQACMRGSDFYEGVRAVIIDKDQNPKWKPSTLAEVTEQLVEESFRSLGDRDLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CUJ2</accession>
    <name>A0A3B4CUJ2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_037397950.1">
        <property type="nucleotide sequence ID" value="XM_037542053.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397951.1">
        <property type="nucleotide sequence ID" value="XM_037542054.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037397952.1">
        <property type="nucleotide sequence ID" value="XM_037542055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CUJ2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015013"/>
    <dbReference type="Ensembl" id="ENSPNAT00000023071.2">
        <property type="protein sequence ID" value="ENSPNAP00000015013.2"/>
        <property type="gene ID" value="ENSPNAG00000021034.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430618"/>
    <dbReference type="GeneTree" id="ENSGT00940000156528"/>
    <dbReference type="OMA" id="ARRQTMW"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000768">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000705">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF3">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="13">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="426"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="95"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="300"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="372"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="387"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015013.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="700" mass="77971" checksum="9100A16A192CE1D3" modified="2024-11-27" version="2">MFRKKKKKRPEISAPKNFEHRVHTSFDAKRGVFVGLPTQWQSLIENLRRPKPMVDPSRITPVELKPKKTIVRGSFVGHGDYISAMLSDMSRLSVTSSNSLRKSSPSTRKRAQSLGRLGEVIEGDTYQYEDLEQGDPEADAQAKWRDQSRNVHSESGTPYMGMKKSITLQPNGVLPRAKSTYEVSVSSLEGRPVPPALPAPPPPPIPVPPRPVYVGGDLGSPNERLMMRREFPIVLSHNPRPISCFYSPTMPMQHAQGESGGLPPDLRTTDRLLVHAQNSPGRPYSSYDLKVQADTALRPQPGFFHSGASSPLVSATRPHRTVRSSSSYTIGLSPNISFRPAGPDPFLRHSGCPNPGLYPRQDSPSQPRPSPTGSLATSPPGTCSPAFRPPQHPSPRPPPDPPKVTHEQFKAALQMVVDKGDPRSYLENFVKIGEGSTGVVCIAREKHSGRLVAVKMMDLRRQQRRELLFNEVVIMRDYQHKNVVEMFKSALVEEELWVIMEYLQGGALTNIVSETRLSEEQIATVCEAVLQALAYLHSQGVIHRDIKSDSILLSLDGRIKLSDFGFCAQISKDIPKRKSLVGTPYWMAPEVISKSPYGTEVDVWSMGIMVVEMVDGEPPYFSETPVAAMKRLRDEPAPTIRNIQQVSPVLKDFLDRMLTRDPLERASATDLLEHPFLLQSSSPQCLVPLVEQYRKRMSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVW2</accession>
    <name>A0A3B4CVW2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVW2"/>
    <dbReference type="Ensembl" id="ENSPNAT00000037760.2">
        <property type="protein sequence ID" value="ENSPNAP00000015500.2"/>
        <property type="gene ID" value="ENSPNAG00000020805.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182969"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
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        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
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        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF367">
        <property type="entry name" value="KERATIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="4">
        <location>
            <begin position="84"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="196"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="288"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015500.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="438" mass="48290" checksum="727E1B0D9FF15715" modified="2024-11-27" version="2">PSPEGLKGTRLLCSPLIPLQSEQKGTESLAAPCPLLSASSIQLGSRRAGSVCGGAGGSGVRISTASSVVSGFNMADTLDVSANEKATMQNLNDRLAAYLEKVRSLEKANADLELQIHQFLESKASPKAWDYSAFYISTDVFISILQIQNAIAVNGGICLGIDNAKLAADDFRVKYENELAMRQSVEADIAGLRKVLDGLTLVRLDLEMQIEGLREELICLKKNHEEELLAMRSQMGGHINVEVDATPQDDLSVVLAGIRKHYEAVAAKNRKDLEAWYQTKIFSLTETLQTSRSEITEVKHTLQSLEIELQSQLSMKASLETTLTEIQSRYTRMLAGYQVQVTSLEEQLAQLRADLESQSLEYQMLLDVKTRLELEIAEYRRLLDGETTTTTTDSPTITESVNHVGSCNQINVLHNIKDLKSPVEKLQNTVPHLVTWAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CVW5</accession>
    <name>A0A3B4CVW5_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GPR82</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CVW5"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016247"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024689.2">
        <property type="protein sequence ID" value="ENSPNAP00000016247.1"/>
        <property type="gene ID" value="ENSPNAG00000022378.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282516"/>
    <dbReference type="OMA" id="QKNTCIG"/>
    <dbReference type="OrthoDB" id="9946711at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042804">
        <property type="entry name" value="GPR82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47392">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 82-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47392:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 82-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
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        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="325"/>
        </location>
    </feature>
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        <location>
            <begin position="343"/>
            <end position="381"/>
        </location>
    </feature>
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        <location>
            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="381" mass="41967" checksum="0F53672A4106DAFE" modified="2018-12-05" version="1">MAVCLQLTTSNSSMCLCQTFTTHLFLPILYSAMFLTGLAGNVLSLWIFMAKISGKSPTHIYLINLGISNLLLCITMPFIAVYYALGTSWSSRSLMCRLAINVLTPVLHTNIGVGMIILAWLALSRCATLVQHSHGPRPSKCIKILPKVFFSRLRQTPFALGVCMGTWAIVATASIPSVVVYSMKETEVVEDGDKQTCYSVAVEVGGSGSQAFAVIGIVIFFLCLLVVLCAYMAVIRHFYRSRKSTVISDRQRVYARVFRNIVVIQIVLVVCLLPYHIYKAIFINLAQTDSGGNGTECHSLSTYVEIKNVLLCLAALRCSTDPIMYFLLDKTFKKHTLNLIRGRSNTQDSHSSRTECGQSAPYNNSSPKGAATHDTHSHSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CW31</accession>
    <name>A0A3B4CW31_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4CW31"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000016307"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038077.2">
        <property type="protein sequence ID" value="ENSPNAP00000016307.2"/>
        <property type="gene ID" value="ENSPNAG00000022462.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156607"/>
    <dbReference type="OMA" id="HQQHMQN"/>
    <dbReference type="OrthoDB" id="6846267at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007416">
        <property type="term" value="P:synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000001">
        <property type="entry name" value="Neuroligin 3 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000460">
        <property type="entry name" value="Nlgn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01090">
        <property type="entry name" value="NEUROLIGIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043915737" description="Carboxylesterase type B domain-containing protein" evidence="15">
        <location>
            <begin position="40"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="16">
        <location>
            <begin position="42"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="646"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="734"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000016307.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="861" mass="96803" checksum="17C730A7E0E83CAC" modified="2024-11-27" version="2">MSRQGGTGWMPSTMVQPFFTLPRISKIVVVLVWLAMAQAQQHPIVTTNYGKLRGLKTALPNEILGPVEQYLGIPYALPPTGERRFQPPEPPMSWPGIRNATQFAAVCPQFLEDRFLLNDMLPVWFTANLDTVVTYVQDQSEDCLYLNIYVPTEDGANTYNQGDEFTNNEGGEDEDIHDENGLRPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVVTLNYRLGVLGKSSVWFNVMLVLDWFLSTGDQAAKGNYGLLDQIQALRWIKENIQAFKGDPKRVTIFGSGAGASCVSLLTLSHYSEDLFQKAIIQSGTALSSWAVNYQPAKYTRMLAEKVGCNMLDTIDLVECLQKKNHKELIEQYITQAKYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGFKFVDGIVDSEDGVSANDFDFAVSDFVDHLYGYPEGKDTLRETIKFMYTDWADKENPETRRKTLVALFTDHQWVAPAVATADLHAQYGSPTYFYAFYHHCQSEMKPSWADSAHGDEVPYVFGIPMIGPTDLFNCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVAFWLELVPHLHNINDLFQYVSTTTKIPPQDTTPFPYTKRLGKTWPSTTRHPGVPPPNPKQTTDQRKGSDLGDDSAVVIENKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHESNRRGPSPQRNSAPANAAANANDIARLQNEELMSLQMKQQQQLEHEHHHECESLQAHDTLRLACPPDYTLTLRRSPDDIPLMTPSTITMIPNSLAGMQPLHNFNTFGGNQNSTSLPHGHSTTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4CXD4</accession>
    <name>A0A3B4CXD4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Terminal nucleotidyltransferase 5B</fullName>
            <ecNumber evidence="4">2.7.7.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Non-canonical poly(A) polymerase FAM46B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:11333"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TENT family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017579586.1">
        <property type="nucleotide sequence ID" value="XM_017724097.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4CXD4"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000015993"/>
    <dbReference type="Ensembl" id="ENSPNAT00000024353.2">
        <property type="protein sequence ID" value="ENSPNAP00000015993.1"/>
        <property type="gene ID" value="ENSPNAG00000022093.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108443429"/>
    <dbReference type="CTD" id="777768"/>
    <dbReference type="GeneTree" id="ENSGT00940000165028"/>
    <dbReference type="OMA" id="RYDCLMT"/>
    <dbReference type="OrthoDB" id="10065073at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012937">
        <property type="entry name" value="TET5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12974">
        <property type="entry name" value="PRION-LIKE- Q/N-RICH -DOMAIN-BEARING PROTEIN PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12974:SF46">
        <property type="entry name" value="TERMINAL NUCLEOTIDYLTRANSFERASE 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07984">
        <property type="entry name" value="NTP_transf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01153">
        <property type="entry name" value="DUF1693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000015993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="393" mass="44868" checksum="2BB921B935F2FC77" modified="2018-12-05" version="1">MSPGDASEQSRRFCVLSWEQVQRLDSILGETVPIHGRGNFPTLSVQPRQIVQVVRARLEERGVTVRDVRLNGSAASHVLHQDTGLGYKDLDLIFGVSLSDDQAFHLVKDVVLDSLLDFLPEGVSKERITALTLKEAYVQKLVKVCNDTDRWSLISLSNNTGKNVELKFVDSLRRQFEFSVDSFQIGLDSLLLFDRCSETPMSESFHPTVLGESVYGDFEEALSHLCEKIIATRSPEEIRGGGLLKYCHLLVRGFQPRSETEMKSLQRYMCSRFFIDFSDISEQQRKLEAYLQNHFAGMEHKRYDCLVTLYHVVNESTVCLMGHERRQTLNLISMLALRVLAEQNAIPTVTNVTCYYQPAPYVRDINFNNYYIAHVQPLIHTCSNSYQTWLPCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D266</accession>
    <name>A0A3B4D266_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NR5A1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D266"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018427"/>
    <dbReference type="Ensembl" id="ENSPNAT00000038953.2">
        <property type="protein sequence ID" value="ENSPNAP00000018427.2"/>
        <property type="gene ID" value="ENSPNAG00000024839.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153391"/>
    <dbReference type="OMA" id="YPYPEVY"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06944">
        <property type="entry name" value="NR_LBD_Ftz-F1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000011">
        <property type="entry name" value="Nuclear receptor subfamily 5, group A, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF48">
        <property type="entry name" value="FF1D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002530">
        <property type="entry name" value="Nuc_orph_FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="235"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="360"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR002530-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018427.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="482" mass="54000" checksum="D23BD449E25D2D1B" modified="2024-11-27" version="2">MEYGYDADLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKRYTCAESQDCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRASGLKMETTPLLQLPVQTDYSFGSMLPSLPPVSTPKHALSTTPSSTATAADYSLYTPGCMPVPAHGPLSPQYPYTSFPSRAIKSELPDHYASASSPDSALGFTYSEHTQTGSPSAPPAGLPVPALVLEFVRCEQDELQVRSKISAHLGHLQQEQSSRSAKAEPLSTFGLMCHVADQTLFSIVEWARSCTFFKELKVGDQMKLLHNCWSELLVLDYISRQVHHGKQDSILLITGQEVELGSLLAQAGVTLAELVQGGQELVQHLQELQLDRREIACFKYLILFNPDVKLLENQVYVESVYEQVSGALLEYTLCVYPQHVERFSQLLLRLPELRALSVQAEDYLCYKHLSGEVPCNNLLIEMLHAKRACV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D283</accession>
    <name>A0A3B4D283_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc finger CCHC-type and RNA-binding motif-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">U11/U12 small nuclear ribonucleoprotein 31 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RTF2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D283"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017633"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026518.2">
        <property type="protein sequence ID" value="ENSPNAP00000017633.2"/>
        <property type="gene ID" value="ENSPNAG00000023955.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111061"/>
    <dbReference type="OMA" id="AHYFNDE"/>
    <dbReference type="OrthoDB" id="267048at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000233">
        <property type="entry name" value="Zinc finger CCHC-type and RNA-binding motif-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000009">
        <property type="entry name" value="Zinc finger CCHC-type and RNA-binding motif-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044598">
        <property type="entry name" value="ZCRB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46259">
        <property type="entry name" value="ZINC FINGER CCHC-TYPE AND RNA-BINDING MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46259:SF1">
        <property type="entry name" value="ZINC FINGER CCHC-TYPE AND RNA-BINDING MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="64"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="14">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="174"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000017633.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="272" mass="30706" checksum="B675F7E4C3C3577A" modified="2024-11-27" version="2">MVSRACILNVRFPVLLWRGSNVCVDADDPPALSGVHVILCARFGLKLQLFVRCNMSGGLAPSKSTVYVSNLPFSLTNNDLHKLCTKYGKVVKVTVVKDKQTRMSKGVAFVLFLDRESAHNCARSLNNKQLFSRTVKASIAVDNGRAAEFIRRRNYSDKSRCYECGEDGHLSYSCPKNLLGEREPPPKKEKKKKKKIKHTEEVEPEESEDEGEDPAIDSLSQAIAFQQARMEEEERRRKRATEDSSQASTSEDSHKPRIKKSTYFSDEEELSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D2N3</accession>
    <name>A0A3B4D2N3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Delta-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Putative Notch ligand involved in the mediation of Notch signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Membrane</location>
            <topology evidence="1 18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D2N3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000017778"/>
    <dbReference type="Ensembl" id="ENSPNAT00000026785.2">
        <property type="protein sequence ID" value="ENSPNAP00000017778.2"/>
        <property type="gene ID" value="ENSPNAG00000005853.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164418"/>
    <dbReference type="OMA" id="VLCRCQA"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021536">
        <property type="term" value="P:diencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000368">
        <property type="entry name" value="Delta-like 3 (Drosophila), isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000064">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.140:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3510:FF:000004">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
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    <sequence length="600" mass="66160" checksum="5BCB4138E199AA61" modified="2024-11-27" version="2">MEMILMCMFELKVHAFSTSRRFCRRPRDCNIFFRICLKHSEDVISAEPPCTYGTGQTGVMRAEHSAIANSAAIRVPFHFKWPGTFSLVIEAWNAESPKEHPDYTENQNNLISRLATRRRLAIGEDWSQDVHFGEQSELRYSYHVFCDEFYYGDACSDYCRPRDDTLGHYTCDENGNKQCLEGWQGDYCSDPICSADCSDRHGYCESPGECKCRLGWQGLSCSECVRHPGCLHGTCTQPWQCVCKEGWGGLFCNQDLNYCTNHRPCANGATCTNTGQGSYTCTCRPGYGGTNCELEINECDCNPCKNGGSCNDLENDYSCTCPQGFYGKNCEIVAMTCADDPCFNGGTCEERFTGGYVCRCPPAYTGSNCEKKLDRCSHKPCANGGDCVDVGSSALCRCRPGFSGPLCEVNVDDCLHSPCQNSGTCVDGVNDYTCSCTLGFTGKNCSVRAHACLTHPCLHGGTCYTHFSGPVCQCVPGFMGPSCEFPVLERGSSPSTVAASCVLGVLAVCLGICVGLVVLRRRRQRLRRRQLCDSVFNDLETVNNFDRQQQYPYERDFLTRVVSQAKPSNTEARLSCPLAPAHTLPAGRDFLWSAGGVGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D451</accession>
    <name>A0A3B4D451_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">SLIT and NTRK-like family, member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SLITRK4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLITRK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4D451"/>
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    <dbReference type="Ensembl" id="ENSPNAT00000027481.2">
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        <property type="gene ID" value="ENSPNAG00000024697.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161248"/>
    <dbReference type="OMA" id="DICKTRC"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SLIT and NTRK-like family, member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="LRRNT"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4-RELATED"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043904634" description="SLIT and NTRK-like family, member 2" evidence="11">
        <location>
            <begin position="35"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="636"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="12">
        <location>
            <begin position="41"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="13">
        <location>
            <begin position="229"/>
            <end position="277"/>
        </location>
    </feature>
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        <location>
            <begin position="355"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="13">
        <location>
            <begin position="545"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="278"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="595"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="753"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="608"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="786"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018325.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="803" mass="89876" checksum="7737E8F95D467873" modified="2024-11-27" version="2">MPGGVSALRAVKMLNNILLLSVLTVSGFSSRGESRKTSKDICRNRCSCEEKESMLNINCENKGFTSVSAFQPPQNKVSQLFLNGNFLTKLNPNEFANYGNVTSLHLGNNGLQEIKTGAFNGLRNLKRLHLNNNHLEIIKEDTFSGLESLEYLQADYNYISAIEAGAFNKLNKLKVLILNDNLLLSLPNNIFRFVMLTHLDLRGNRLKTLPFAGVLEHIGGIMEIQLEENPWNCTCDLIPLKAWLDTIAVFVGDIVCETPFRLHGKDVTQLIKQDLCPRRGPASEAGHRAVQPPPDAHHRAPSPTVRARVTPSRAPKASRPPKMRHRPTPRVTPGRDRHVFGPIMVYQTRSPVPMSCPSVCVCTAQNPDSGLNVNCQERKLHNISELQPKPSYPKKLHLTGNYLQTIFRMDLAEYSSLELLHLGNNRIAIIQDGAFENLTSLRRLYLNGNYIESLSQSLFAGLQSLQYLYLEYNIIKDILPNTFNSLHNLQLLFLNNNLLRSLPDNVFGGTMLTRLNLRNNHFSYLPVRGVLDQLSAFIQIDLQENPWDCTCDIVALKNWMELSSTSVVVNEITCDSPSKHAGRLLRSLRNDAICPDTGEAPPTKAPTVISSSTGETPFSVATPTDRVPETHPEVPLSVLILGLLVVFILSVCFGAGLFVFVLKRRKGVDSVPSGANNLDMSSFQVQYGSYTTEPSVNKSENRVYNYIPPPVGQMCQNPIYMQKESEQVTYYRNLKELSFSTLDPKKEELARSPGYTKYLGSGPHEHRQWQKWQGKNLEEEEETSRGTKTQKGNPLPSGNTRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4D5W3</accession>
    <name>A0A3B4D5W3_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 164</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4D5W3"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000018905"/>
    <dbReference type="Ensembl" id="ENSPNAT00000028392.2">
        <property type="protein sequence ID" value="ENSPNAP00000018905.1"/>
        <property type="gene ID" value="ENSPNAG00000025415.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108429438"/>
    <dbReference type="GeneTree" id="ENSGT00390000007430"/>
    <dbReference type="OrthoDB" id="17328at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026508">
        <property type="entry name" value="TMEM164"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20948">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20948:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14808">
        <property type="entry name" value="TMEM164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000018905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="35499" checksum="773C76FCB6880F28" modified="2018-12-05" version="1">MFRQEVSVLSFIPSCSYLFQYEKRNMSRYNIYSLLDWVYGGVDPKFEGNGGPECAAFITPQQRLCESLIIVVLSVVEVVAALRKINIAKQHREAVKDGRNNKEDSLGKNLLLVALCMTFGVEVGFKFATKTVIYLLNPCHVVTMVQIFLLACPPGKTAMVIFRLQVHMLNGALLALLFPVVNTRLLPFEKEIYYIQHSMLYLVPIYLFRKGGVYKPEPLSDMWWAVLSTGILFLYHFVFLQGLGMVTEVNLNNMLCPAVSDPFYGPWYRVWATGHQTLLTLVHGKALTILSHHTAPALRHLLDAARLRTKKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DAK7</accession>
    <name>A0A3B4DAK7_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DAK7"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040049.2">
        <property type="protein sequence ID" value="ENSPNAP00000020478.2"/>
        <property type="gene ID" value="ENSPNAG00000006438.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222294"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="89"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000020478.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="188" mass="19962" checksum="3654A291485DBC81" modified="2024-11-27" version="2">MKLSPSVSVSISPSGVILEGSSVTLTCSSDGNPPVEYNWIKGTSSVGKGETYTMKKISSVNSGEYKCRSSNEHGEKLSEALTLNVLYPPKSVSVSISPSSETAEGSSVNLTCSSDANPPVQNYTWFKEGGSSPVGSGHSYMAPQRGSYYCEAQNEHGSQKSAAVTVKGVIDGKHTSCYKQRVKYSEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DCD4</accession>
    <name>A0A3B4DCD4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ZDHHC6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B4DCD4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040378.2">
        <property type="protein sequence ID" value="ENSPNAP00000021140.2"/>
        <property type="gene ID" value="ENSPNAG00000028163.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155642"/>
    <dbReference type="OMA" id="GCIHAAI"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="96"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021140.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="419" mass="48279" checksum="18B43D3443D6BCF5" modified="2024-11-27" version="2">MNILSAIIVFENLHEVRRLCHWGPIIALTVIAVCSSMAILDSIIWYWPLDTTGGSINFIMLINWTVLILYNYFNAMFVGPGYIPLGWKPENQQDTMYLQFCRVCQGYKAPRSHHCRKCNRCVMKMDHHCPWINNCCGHLNHAYFTTFLLLAPLGCTHAAFIFIMTMYTQLYDRISFGWSSVKIDMSAARRIHHPIMPFSVASFAATLFALGLALGTTIAVGMLFFIQIKVIMRNKTSIEVWIEEKAKDRIQYYQTGEEFIFPYDLGSRWENFKQVFTWSGTPEGDGIEWPVNEKCHQHTLTIEQLKQKADKRVRSVQYRVVENYNGACCPLRKGFNTFLRTPCTEEPRIQLSKGETIFATRGTNGIVYICYLLNMFVAFFVSALLAAVRVRGWFPRRCVEKCLYDSANNTSASEEKKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DE73</accession>
    <name>A0A3B4DE73_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DE73"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021753"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040762.2">
        <property type="protein sequence ID" value="ENSPNAP00000021753.1"/>
        <property type="gene ID" value="ENSPNAG00000028946.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167100"/>
    <dbReference type="OMA" id="KPRWKQS"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000002">
        <property type="entry name" value="Matrix metallopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF316">
        <property type="entry name" value="STROMELYSIN-2 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="23">
        <location>
            <begin position="126"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="357"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="21">
        <location>
            <begin position="405"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="20">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="17">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="19">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="313"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="498" mass="55859" checksum="FE7B979E2CE238FB" modified="2018-12-05" version="1">MTAGHISSSAPLKEKQEMENMGLLTLTLAVCLVLCDGAPASVTQETVSTEDQEAAEAYLKHFYSDSSTSSVSGRRVSIQDFETTLKQMQEFFGLEVTGKLDTNTIKVIKKPRCGVSDVAEFGHFPGKPKWEKKTITYRITEYTPDLSQRDVDAVITQAFKLYSDVIPLDFKQVYSGTADIMILFKARYHGDFHPFDGPSGVLAHAMSPGPDVGGDTHFDDDEQWTLSAAGVNLLLVAAHEFGHALGLDHSRDPSALMYPTYQYVNTNGYRLPLDDKRGVQALYGVRESPYPQPQPEPNPQPKPEPGPNPPEPCKRDLVFDAATSIRGELYFFKNEYYWKKTSSSIQQIRIKSTWPAINSVDAAYEFKSKGVSYFFKGQQYWGVKGPVSLPGYPKPISQFGFPSSVTKIDAAVYVKSTGRTLFFVGSNYWSFNERTGKMDRGFPKPIHHDFPGIGSRVDAAFENYGYLYFSDGARQTEYEYKKGRVRRVLLNYGWLNCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DES8</accession>
    <name>A0A3B4DES8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SLAIN motif-containing protein-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SLAIN motif-containing family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017541219.2">
        <property type="nucleotide sequence ID" value="XM_017685730.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DES8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021576"/>
    <dbReference type="Ensembl" id="ENSPNAT00000033215.2">
        <property type="protein sequence ID" value="ENSPNAP00000021576.2"/>
        <property type="gene ID" value="ENSPNAG00000006450.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108413285"/>
    <dbReference type="GeneTree" id="ENSGT00390000017860"/>
    <dbReference type="OMA" id="NTEPGSC"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026179">
        <property type="entry name" value="Slain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22406">
        <property type="entry name" value="NASCENT POLYPEPTIDE-ASSOCIATED COMPLEX SUBUNIT ALPHA, MUSCLE-SPECIFIC FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22406:SF5">
        <property type="entry name" value="SLAIN MOTIF-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15301">
        <property type="entry name" value="SLAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="345"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="465"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="525"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="468"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021576.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="65478" checksum="254664DCB7850F15" modified="2024-11-27" version="2">MVVPDSGNVVQQADNGSTSGLMASAEPGFEVEQDMAEVELEEVRKLQELVRRLEVQNQSLRNRGNKHHLRGTNSNINERTLCEEITSSAVRPEGGGGLTGRGFERSPPPDSSSSEDVSPLPEAARPEDEEDEPGRFLSLPCGAGLEQTQGQPQASPSPDSYDSETLGGSDAGVDQSALDEVDVLDLEDCVETEDEDSWLYVSPKKQVLAEQGPESPLKWCRKVLDHPSPETEVACRTLISRLDQTSRWRNVYCSPSQSASGSTAEAGSSGAPLLSPGYHKSTNKSLLTCGSSGYVGMHSALSSQSSIDSELSTSDDSISMGYKLQDLTDVQIMARLQEESLRQDYASSSASASRRSSTASLQSLRRGTHSDQEFDSYSLEDGEDDCSSLPQRLHRYSPSPRASPRCLSPSTLAEYSRLAAPRTRTPRRSLQGSGPELFKFAKSEEELRHSMPNLAPRTSLRSLEAVRNSRSMEANLQSSGNRMSRLPQSPTGASSSRMRGSGQSPLSLRAPMKALSPVGSMAAIRQNAKGPPGTQGSPAAGARRVQSPGPANGGAYAACRTAAIGPKQTPGRGMAPAAPSSRGRLAQAPRSRSLGMTKTCGPIADDSWKDGCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DEZ6</accession>
    <name>A0A3B4DEZ6_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FBXO41</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017564969.1">
        <property type="nucleotide sequence ID" value="XM_017709480.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037388759.1">
        <property type="nucleotide sequence ID" value="XM_037532862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DEZ6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000021646"/>
    <dbReference type="Ensembl" id="ENSPNAT00000040691.2">
        <property type="protein sequence ID" value="ENSPNAP00000021646.1"/>
        <property type="gene ID" value="ENSPNAG00000028757.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108434399"/>
    <dbReference type="CTD" id="150726"/>
    <dbReference type="GeneTree" id="ENSGT00530000063713"/>
    <dbReference type="OMA" id="CGTTPNC"/>
    <dbReference type="OrthoDB" id="6482165at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="CDD" id="cd22109">
        <property type="entry name" value="F-box_FBXO41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057038">
        <property type="entry name" value="FBX41/ZN365_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052283">
        <property type="entry name" value="GenomicStab_NeuMorph_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15739:SF4">
        <property type="entry name" value="F-BOX ONLY PROTEIN 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15739">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23165">
        <property type="entry name" value="zf-C2H2_FBX41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBX41/ZN365 C2H2-type zinc finger" evidence="5">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="487"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="298"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="399"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000021646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="805" mass="88911" checksum="3577EEE0C1B19C07" modified="2018-12-05" version="1">MASLDLPYRCPRCGEHKRFRSLSSLRAHLEYNHTYETLYVLSKSNSVCDAAALLPLVADSALAAESRFPYRELPCSSDESSSSAARYLPNVEFPLGEILVKKAMSSADAGNANNAVAVAANVAASAVEAAYEEGLARLKARAFERLELDERLEKLSEEVEQKIAARVGRLQAELERKSSELEKAKVESERLSQEKQELEDKASELSRQVDVSVEMLAGLKQDLANKEQELTHKQQEVAQIDQFLQETAAREASAKVRLQQFIEELLDRADRAEKQLQIISSCGTTPNGSLGCCSLQGSKANGRQRNSSISGSRGMYQVSDRRSSPSTGASGRVKSVSQGSAVGYDSDSVDLHALEGSLEAQYYHAQCRLGESGAYERGPVSGGAPRNWGLRKQAIQNWQRRPYRNSTEGEEGDVSDVGSRTTESEAELWEQERRASADSQQHGGHRGGYRLGTGRSEAGYKPCRHEKSPSKSNEVISPEVLKMRAALFCIFTYLDTKTLLRAAEVCRDWKFVARHPAVWTRVLLENARISSKFLCTLSQWCTQTHSLILQNLKPRQRGKKETKEEYLKSTRGCLEEGLEALLKATGGNLLILKVSHCPNLLTDRSLWLASCYCRALQAVTYRSATDPVGQEVIWALGAGCRDIISLQVAPLHPCQQPARFSNRCLQTIGRCWPHLRALGVGGAGCGIQGLASLARNCMRLQVLELDHVSEINQEVAAEVCREGLKGLEMLVLTSTPVTPKALLHFNSVCRNLKSIVVQIGIEDYFEDPNSPEARKLFDEMVNKLQALKKRPGFSKILHVKADGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DG72</accession>
    <name>A0A3B4DG72_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein phosphatase 1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PPP1R12C</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">PP1 comprises a catalytic subunit, and one or several targeting or regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017548471.1">
        <property type="nucleotide sequence ID" value="XM_017692982.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DG72"/>
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        <property type="protein sequence ID" value="ENSPNAP00000022500.1"/>
        <property type="gene ID" value="ENSPNAG00000029893.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424765"/>
    <dbReference type="CTD" id="54776"/>
    <dbReference type="GeneTree" id="ENSGT00940000161425"/>
    <dbReference type="OMA" id="AWGPDTQ"/>
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        <property type="component" value="Chromosome 30"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0019208">
        <property type="term" value="F:phosphatase regulator activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Phosphatase 1 regulatory subunit 12A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 12A"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017401">
        <property type="entry name" value="MYPT1/MYPT2/Mbs85"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051226">
        <property type="entry name" value="PP1_Regulatory_Subunit"/>
    </dbReference>
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        <property type="entry name" value="PRKG1_interact"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 12"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 12C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="PRKG1_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PP1_12ABC_vert"/>
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    </dbReference>
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        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="241"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="274"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="cGMP-dependent protein kinase interacting" evidence="9">
        <location>
            <begin position="600"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="343"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="605"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="343"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="366"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="396"/>
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        </location>
    </feature>
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            <begin position="419"/>
            <end position="437"/>
        </location>
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        <location>
            <begin position="460"/>
            <end position="477"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="8">
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            <begin position="478"/>
            <end position="488"/>
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        <location>
            <begin position="507"/>
            <end position="518"/>
        </location>
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        <location>
            <begin position="578"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00023043"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000022500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="700" mass="77827" checksum="A9EC7A108AC0C03B" modified="2018-12-05" version="1">MGEAARAQRREQLKRWSGSCTDREPTVPRRRWRGEQEEEEGDDDEGGRCRDGNGPVREKKAKRSRRVRFERTAEFLAVCASGDTEEAQAMLKEAWQKSNGELVSGANVVNCANADGITALHQACIDGNMDVVSFLLSQGANVNQVDNEGWTPLHVAASCGNREITEYLLKQGASLCLVNCDGDSPLDIAEDEAMEALLQEHTQKQGVDLEAAKRLEEELIMRDARSWLTDGLPADLQHPRTGATPLHVAAAKGYLEAIKLLCQCGLDVSAKDWDGWTPLHAASHWGQREACSILAEQLCDMEAHSNGGQTPFDVADESLVALLEELLLRQANWRTEQAINDKQNAQAAGNGNAANKRRRSSVCRMSSREKMSVQDQSKERGVSGGLELSEERESSPESSTVSSPDTESVTTSTVSPADKTPEEKEAEEKEQERESRTARVPPTLQRRDSAGESPSSASGDGRKFQAPVRDEESESQRKARSRLMRQSRRSTQGVTLTDLKEAERTVSRNTEPQSNLQPVSPVVTLTPAERDTDPVKQTGTAEGDKLGMRDRRRGRKERRPTGIPSLPGETEDFDVNENVLTDSTSSGPQAEDRKLSLSLDFRKLYLEVLKENATLREKLQETQLQLSESKVELERLRQNQENGCERPALLELERFEKKALQRKASELEDELKILVDLRADNQRLKDENAALIRVISKLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DL38</accession>
    <name>A0A3B4DL38_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trafficking protein particle complex subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TRAPPC13</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPPC13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017580996.1">
        <property type="nucleotide sequence ID" value="XM_017725507.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DL38"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025102"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008452.2">
        <property type="protein sequence ID" value="ENSPNAP00000025102.1"/>
        <property type="gene ID" value="ENSPNAG00000010044.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108444356"/>
    <dbReference type="CTD" id="80006"/>
    <dbReference type="GeneTree" id="ENSGT00390000015280"/>
    <dbReference type="OrthoDB" id="10250284at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010378">
        <property type="entry name" value="TRAPPC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055428">
        <property type="entry name" value="TRAPPC13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055429">
        <property type="entry name" value="TRAPPC13_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055427">
        <property type="entry name" value="TRAPPC13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23643">
        <property type="entry name" value="TRAPPC13_C"/>
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    </dbReference>
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        <property type="entry name" value="TRAPPC13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRAPPC13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 N-terminal" evidence="4">
        <location>
            <begin position="23"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 middle" evidence="6">
        <location>
            <begin position="185"/>
            <end position="315"/>
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            <begin position="328"/>
            <end position="422"/>
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    </feature>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00021500"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF06159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="431" mass="48265" checksum="D4C1A229D0D80C01" modified="2018-12-05" version="1">MKAKVHSGLSQHKMDVNQAKQEHLLALKVMRLTKPTLFTNMPVTCEDRDLPGDLFGRLMREDPSTIKGAETLMLGEMLTLPQNFGNIFLGETFSSYISVHNDSNQVVKDILVKADLQTSSQRLNLSASNSAVAELKPECCIDDVIHHEVKEIGTHILVCAVSYTTQSGDKLYFRKFFKFQVLKPLDVKTKFYNAESDLSSVTDEVFLEAQIQNITTSPMFMEKVSLEPSMMYNVTELNTVASGKECSESTFGKMLYLQPMDTRQYLYCLKPKPEFAEKAGVIKGVTVIGKLDIVWKTNLGERGRLQTSQLQRMAPGYGDVRLSLEFIPDTVNLEEPFDITCKITNCSERTMDLLLEMCNTRSVHWCGVSGRQLGKLSPSGSLAIPLKLLSSVQGLQSISGLRLTDTFLKRTYEYDDIAQVCVVCPYLSPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DL41</accession>
    <name>A0A3B4DL41_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exo-alpha-sialidase</fullName>
            <ecNumber evidence="3">3.2.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NEU4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of alpha-(2-&gt;3)-, alpha-(2-&gt;6)-, alpha-(2-&gt;8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.</text>
            <dbReference type="EC" id="3.2.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 33 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.18" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017547241.1">
        <property type="nucleotide sequence ID" value="XM_017691752.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DL41"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025107"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008440.2">
        <property type="protein sequence ID" value="ENSPNAP00000025107.1"/>
        <property type="gene ID" value="ENSPNAG00000010012.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108424011"/>
    <dbReference type="CTD" id="129807"/>
    <dbReference type="GeneTree" id="ENSGT00950000182944"/>
    <dbReference type="OMA" id="HIDCREC"/>
    <dbReference type="OrthoDB" id="2739686at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004308">
        <property type="term" value="F:exo-alpha-sialidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011040">
        <property type="entry name" value="Sialidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026856">
        <property type="entry name" value="Sialidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628">
        <property type="entry name" value="SIALIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628:SF22">
        <property type="entry name" value="SIALIDASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13088">
        <property type="entry name" value="BNR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sialidase" evidence="7">
        <location>
            <begin position="60"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="452" mass="50615" checksum="1F6504C49385F030" modified="2018-12-05" version="1">MTCCWTMGSPSYFPARSVLFRKEASGVTYRVPALIFLPRSSYFLAFCEERLSPADSQAHLLVMRKGTFYRNCVEWEDMRVLSTACLKGHRSMNPCPVYDAFTGTLFLFFIAVLGHTSEAYQLVTGKNVTRLCYVFSDDHGETWSPAVDLTQKVIGDTIKEWATFALGPGHGIQLKSGRLLIPAYAYHIDCKECFNKVCTTTPHSFCFHSDTHGRVWRFGEAVPGPECVECQMVSVDEEDGTNILYCNARSTLGCRVQALSFDDGAMFQDGQLVQKLVEPRNGCHGSVVGFPAPLLVLQNSPKLLRWIASTVGPPSATSVSIKDPSQNFLTPTWVVYAHPTGPGRHDLGLYLNIRPRDPDSWTGPWVIYEGPSAYSDLAYLELQSDGTPPVTVFACLFENGTRTAYDEISFCIFTLFELIDNIPRNKPHLSGVMSRNRTRKKRRRMCDLCRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DL76</accession>
    <name>A0A3B4DL76_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RASAL3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017571238.2">
        <property type="nucleotide sequence ID" value="XM_017715749.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DL76"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000024208"/>
    <dbReference type="Ensembl" id="ENSPNAT00000010706.2">
        <property type="protein sequence ID" value="ENSPNAP00000024208.1"/>
        <property type="gene ID" value="ENSPNAG00000005291.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108438147"/>
    <dbReference type="GeneTree" id="ENSGT00940000165433"/>
    <dbReference type="OMA" id="TWGSRSQ"/>
    <dbReference type="OrthoDB" id="5572587at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04013">
        <property type="entry name" value="C2_SynGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05136">
        <property type="entry name" value="RasGAP_DAB2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057606">
        <property type="entry name" value="SynGAP1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF96">
        <property type="entry name" value="RAS PROTEIN ACTIVATOR LIKE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25321">
        <property type="entry name" value="PH_RASGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="569"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="860"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="887"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="985"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="948"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000024208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="1114" mass="124295" checksum="F470011ED5D08186" modified="2018-12-05" version="1">MGINKEPEIEQTSPPHEEEAAAQRNCPAEGEVEQPEDGNNPLSTYKWHTGAKSEASSQGTFERLEKGAANKWSKMQNWRKALSEDSGDKSTTAAMVKGGESPTKSEKPVSSKKNPFRRALSEPPGALLSSVLSPSTSASSEASGETAQKGKLRKYLHQVSQRLKRPRLQPRNNTEQDVSPVEPAPAALEPTQLSWSPPQDIPVWDINYCVLQDGQIIIPREEEPLMRMRNRASSCLSSLSLQNLSESHTNLECTPDHVAPVSAPNSKAHSGGVKAKIKRRLQGGAQKKQLSQTGRSGSRESLSIPVGIAESLDLSADRTTVIRPVHSSILGEKYCFEVINSENNHCFGCSSAAERDRWIENLRRTAQPNKDNCERTENSLSLWVYEAKDIPPKRRYYCEIHLDGTLFARTSSRAVGKSSQASDGTTSGGAGGSGNQGGCQLFWGELFELDNLPPVSQVTLHLFRDDDPKKKRHSKDDSVLHPLGSVALSLAEIKGRAFQEKWYSLVPYKTPVSGGMKDQLGSQASLRVKARFQNLLVLPIEKYKEFAEYVTVGYVGMCSSLEPLLNVKDKEELAGALVHVLQSIGKAKEFLIHLGSAEVQRLGENKALIFRENTLATKAIDEYMKLVGQKYLIDTLGDIIAQLYASSESCEVDPQKCSSSDLPVNQQHLKKTCSEVVQQITEMHRSFPVELNEIFSSWLSDCEEQGREEIGHRLITASLFLRFLCPAILSPSLFGLTQPYPEPNTLRTLTLTAKVVQNLANFTPFGEKEEYMLFMNDFLEQHWELMRGFLRMVSNPDSELDMARFDGYVDLPLRLAVLHGLLVDIISPMAQDTIDSLQPLPSILNQITDHLGPDAPRITCSSFSLGQPKPTYVPPRDLAKYSPLNKSLQHLPTDSKPSHSRDRQRKQVTRTQSVPTRKHFHPLKRQTSSEELQKNQPEPEEGAGPQMAISSPQDNTRAKGSSIPWVILNKEPNQTKTEQEEMSLLDRHVQELSELRQRVEEALDTELDVAKRLEEYIAQSQGQNAQLQAEVQELRNLLATREEQLASATFRLGVIEEEREEDERKLTIALAAAERMNVLEEQFAGLLNDMQKLNMAHNDDHLTQDRRNTVSGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLA2</accession>
    <name>A0A3B4DLA2_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558322.1">
        <property type="nucleotide sequence ID" value="XM_017702833.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLA2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000025172"/>
    <dbReference type="Ensembl" id="ENSPNAT00000008315.2">
        <property type="protein sequence ID" value="ENSPNAP00000025172.1"/>
        <property type="gene ID" value="ENSPNAG00000001364.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430370"/>
    <dbReference type="CTD" id="100884131"/>
    <dbReference type="GeneTree" id="ENSGT01150000286935"/>
    <dbReference type="OMA" id="CYAHIAY"/>
    <dbReference type="OrthoDB" id="9996086at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15336">
        <property type="entry name" value="7tmA_Melanopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000083">
        <property type="entry name" value="Melanopsin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 14" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="12 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="77"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="382"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="581" mass="63826" checksum="697A050C6D8A2094" modified="2018-12-05" version="1">MSTALSTPQPDSSSTAGAPWNHSGASAYKSAALPERATSVAMVQDSQYPFPTVDVPDHAHYTIGSVILVIGITGMVGNFLVMYAFCKSRSLRTPSNMFIINLAITDFLMCITQTPIFFITSMHKRWIFGEKGCELYAFCGALFGMCSMITLMVIAVDRYFVITRPLASIGKMSHKRALIILVIAWLYTMCWSLPPFFGWSAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFVFVFFIPLFVIMYCYFCIFRAIRNTTSAVGKISAEGGTTKDSIKKFHRMKSEWKMAKVALIVILLYVISWSPYSCVALTAFAGYADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYRSALAKYIPCLGVLLCVPRRDRFTSSSFMSTRRSTITSQSSEIRHTSKTRLSSVSDSESGLTDTEADLSSATSRSASRQVSYDIRKELTDYRQSSSLKVKAKGQNSGGFDRNCSQSPLEPGLNRTTCAYISTVADKDDISMTDVSQSNHLSPTPHVISEQAEHKNLSALIPSIVVTSESSHALKSDGASGHPSKCLPGASTVTSVTMEPFGLDNRKISQPDSITNTAVPLDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLK8</accession>
    <name>A0A3B4DLK8_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Family with sequence similarity 189 member A1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FAM189A1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ENTREP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017558204.1">
        <property type="nucleotide sequence ID" value="XM_017702715.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLK8"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023814"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011810.2">
        <property type="protein sequence ID" value="ENSPNAP00000023814.1"/>
        <property type="gene ID" value="ENSPNAG00000008286.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108430299"/>
    <dbReference type="CTD" id="436705"/>
    <dbReference type="GeneTree" id="ENSGT00530000063335"/>
    <dbReference type="OrthoDB" id="10036151at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030431">
        <property type="entry name" value="ENTREP1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615:SF6">
        <property type="entry name" value="PROTEIN ENTREP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615">
        <property type="entry name" value="PROTEIN FAM189A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="366"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="541"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="697"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
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        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="583"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="79879" checksum="CCE5AF42F8160E43" modified="2018-12-05" version="1">MPVGSVPRARSLSPVSLARSLSRLREFRTRTRIMLSLGVAQMVLGSLILAVSFAALALTTSPRVRHSCPFWAGFSVLLSGLIGVVSWKRPLSLVITFFMLLSAVCVMLNLAGAILSCQNAQLVNSLEDCQLIKFDSDGVCVCCELQHQSSSCNNLGETLKLNPLRDCNTIRLRLKELLFSVCALNVISTIVCALATAMCCMQMVSTDILQMFMPHRARALSADCMTPHGTILHQTLDFDEFIPPIPPPPYYPPEYTCTPVLEGQRGLHLDFPHSPFSAIYGVPINSPGTLYPSELPPPYESVVGQTPASQVTTSLEQQATESSMCERNTTAGLSTQASVDSASLMVSELADIPDRSCSSEDLCSLEVQGSDSSPYGTLHTAPTDGSCTSLEHSVRSSMRREARSPACLDHSEPSIAQDSATRSRENMDALKRDDTPASVHSRDRASTKLTLPVPPATEPQPCASSSLACPSPSPSGRPKGTRLCFSVWSPQPSSTSAHSSSTTSERPRTLRARRYRKLARIVRSTSDPVSCNSITRSESCGCTSVGNQPQQESPQTPSDQACMELSGGTVSQSVKPSSRGAKKQKDGGKVDLRLKALHPTSTERPHSLADLKTYKDTKILVARFLEHSSCSLPPEVQQVVNSIKYVIKSDEKHMEEAIFSASILDQVMSQSRRILSSPRKHAHEDLHLQSCGALSSLSPSLRRPQHRGTGQSAPAASPGHQAPYRPQSLVSVCRETIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DLN2</accession>
    <name>A0A3B4DLN2_PYGNA</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MCOLN3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B4DLN2"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000023839"/>
    <dbReference type="Ensembl" id="ENSPNAT00000011631.2">
        <property type="protein sequence ID" value="ENSPNAP00000023839.1"/>
        <property type="gene ID" value="ENSPNAG00000008347.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108441545"/>
    <dbReference type="CTD" id="100001113"/>
    <dbReference type="GeneTree" id="ENSGT00950000183036"/>
    <dbReference type="OrthoDB" id="263481at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072345">
        <property type="term" value="F:NAADP-sensitive calcium-release channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000033">
        <property type="entry name" value="Mucolipin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049134">
        <property type="entry name" value="MCLN_ECD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039031">
        <property type="entry name" value="Mucolipin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12127">
        <property type="entry name" value="MUCOLIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12127:SF5">
        <property type="entry name" value="MUCOLIPIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCLN_ECD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="391"/>
            <end position="414"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Mucolipin extracytosolic" evidence="15">
        <location>
            <begin position="111"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="14">
        <location>
            <begin position="394"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
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    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21381"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000023839.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="586" mass="67970" checksum="2E7BAE4F1E8508DC" modified="2018-12-05" version="1">MEDFGWVTDCEMQFVIQHSSDQSSAMDDPDAPYLAFNDLNSNGGSMWSDILARQEQREVESFRRKLKYFFMNPCEKYRARGRKPWKLMLQIIKIAVITIQLVSFGLSNQMVVTFKEENLMAFKHLFLKDYTHHSSNSYAVYTQAEVYSHLSYIVDRFLRLRNITVGNHAYEKDGHYFTPLLMCQQFYHNGSILPGNETFEIDAQVEEECLEIYPVQLPTTMEGADLSNFTLQFKRLLEVNVYFTLKAINLQTVQHHELPDCYDFSITIRFNNRAHSGQIKVDLDNDVDINECRDWKVTGASPKNIYYTVLFDVVIILTCLASLVLCTRSVSTGVQLQFEYRAFFLRHFGKLVSWSDRMEFINGWYILIVISDILTITGSVLKIAIQLKALTSYDICSILLGTGTMLVWIGVLRYMGYFKKYNILIITLRAAFPNVIRFCCCAAMIYLGYCFCGWIVLGPYHDKFRSLNTVTECLFSLINGDDMFATFKNMQQKSYLVWLFSRVYLYTFVSLFIYMVLSLFITLITDTYETIKQQQSDGEPVSELQAFISECKDHPSSGCYRVEGRSSTCLFCCVRRPNDLQERLEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DQ27</accession>
    <name>A0A3B4DQ27_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Polycystic kidney disease 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polycystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="42514.ENSPNAP00000025583"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007258.2">
        <property type="protein sequence ID" value="ENSPNAP00000025583.2"/>
        <property type="gene ID" value="ENSPNAG00000010826.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167780"/>
    <dbReference type="OMA" id="PWRQSYL"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="CDD" id="cd01752">
        <property type="entry name" value="PLAT_polycystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000012">
        <property type="entry name" value="polycystin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000434">
        <property type="entry name" value="PC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002859">
        <property type="entry name" value="PKD/REJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042060">
        <property type="entry name" value="PLAT_polycystin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014010">
        <property type="entry name" value="REJ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730">
        <property type="entry name" value="POLYCYSTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730:SF2">
        <property type="entry name" value="POLYCYSTIN-1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PKD"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02010">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00500">
        <property type="entry name" value="POLYCYSTIN1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51111">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2423"/>
            <end position="2443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2628"/>
            <end position="2648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2675"/>
            <end position="2699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2706"/>
            <end position="2725"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="11">
        <location>
            <begin position="606"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="11">
        <location>
            <begin position="714"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="11">
        <location>
            <begin position="787"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="11">
        <location>
            <begin position="979"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="11">
        <location>
            <begin position="1229"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="11">
        <location>
            <begin position="1570"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="domain" description="REJ" evidence="13">
        <location>
            <begin position="1636"/>
            <end position="1979"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="12">
        <location>
            <begin position="2466"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="3023"/>
            <end position="3099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="3127"/>
            <end position="3170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="3035"/>
            <end position="3047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="3076"/>
            <end position="3099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="3143"/>
            <end position="3155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025583.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3170" mass="350428" checksum="72BC347B904B5C06" modified="2024-11-27" version="2">LTGEIVRKLELSISYGYAFGSVSILGAPIKGAGHYRSPPPHYGPKYSFPLVIIPFLLSQATAVRCFNCFSPRGCSYFWISAFSEFFSVFLLMFNRLINTLFIKSHPPDEDSCPKRSKIDQDGLHCYWISASASTWLEARDLCQRWKGGDIAVVDKPSVQDFIDKSFQALGLVKLTYIKSLSLSLSLSLSLSLSLSLSLCLCFSGCSSLLNPFSCCSEEPMCNTTGVCISGLYWCHLLESCLPVTSPCSPYHSSAAGLVFPLPPRYTAVPPFYHMMADVSLKILPASEPVHLRVTVQHSRKAGEFLHCVSSLNSAWRQSYLYLPGPELGGWVEVGVSVPTDGGQWVDNVVCDLQLIYEDSMLVYGVPPVLSSTQSDLRIETTTPTTHLVKPQTPVSGLHVLYPVLDKENQIHLPVNVRTIIIVKILSGHNATSAWSAPVFRTGIPFQLSCPPDMSEIQAVCERDTPDVWFSYIYIKLSAQGEQTLNITASNTLNTQMLSVKLQAHIPVTGLNVQPQGFHRVLVDIPQVLLFLFIIISTYSTCVNHTFSAPLERSNTSLDQTAIQIYLQDSVLHTYTTPGDYTLRIEAENKYDHIKKVVPIKVRSPLTRVLISSMPTVPNVNQSILFEASSLPSSYGILYLWNFGDGSQELKGPYKVSHAFKAAGVYNVSVCSNNTLSQIIAWMSLEVVETISGVHLSYNGPSELNSVTEIYGRVSTGTSLRWTFELGDGSVFKDLSKSSISHIYKSVGNYTAQVTVSNAVSKVRQSINVEIYQLAINGIWPSGCIVSGEEVILQAVVTGNVSSLTFHWSFGDGTLLSVKTGTSTIVHTFSDPRNYLIDLKVYSHVGSVHYQATVCVEALITDLNLHSALNAVAVDEEICFDALVRPSGEMYQFQWYNSSSSDSPVSGKSHHCFFFALEGMHEIMLITHNQVSRKRAKATIFVQRPVLKLSIKHEDNSDMLTVNQSYSFWTEPYQNNGVVEWDFGDGSQMKEGQNQSHTFTSAGRFHVTASVFNAVSQQFVEADVEVQVPISNIVIHTNQPYAEVGQETVFTALCNVVDNVKYFWRVDPLFPSKLGTSEYRYVFPRAGIFEVKVVAQNLVSKMETSASIEIVERIQGVQITNQILKSMRYIPTKETITLTAFVKNGSNLTYNWLIHQDGFNRTVGDGEHFKLFTNNSGNVSVKLTVANVLGREHKDVTLRAVECVSGVNISTPAYVIAKGKPVKITVSVKTGTDLQYIWFLDSDYSPVTTDVPFVLHVFKAIGIFKLRVSVANVLGSVDSTKQLTVQEDISEIDFKVDGKSHPFFVTSNSLLQLHGSVRKGNDLQWVWTVALQGRNVISLGNNQTVSYSFMDVGGHRVTLNASNDISCQSVSHIVTVEDTIQGLNLRVSEMVICENDAVTFTPAISQGSGVSFLLEFAGGNSSLRVLENFTISSLPIGNHTVKAIAENHVSTLSATVAVQVVERVRGLHFCCTTVLEALIPSSFKASVITGSQVTYQWSFQLNGFNTSQETGQNVLYTPFINGSLSVTVKASNAAFCSQSVTETVVVQWPVKKVTLLNSPDGPFIDHPVTFFALADSGSDLMFQWDFGDSDDVVKITKSNTVDHKYKVKGRFVVHVTVFNNVSQVSAQLAVVVSKLECTQPRVSLIQDQPKILKATPNYFEASVELSGCVSYKTIYLWEIYHDPNCTEKKVSLDSSVDVATPLLYLPKQILEVGDYCLKFSARFQRTPLHHQQTTKISVVYSPLVPIIKGGSHRMWSSQNDLILDGTESYDPDSAAHDDLLQLQWDFKVVCVTVYKVSHELICAHNSSSLILPRHILEPGSVYQFTLTVKKDGRPSVSTKQYVKIIPVAVKCVSCSLHFSSYVSHSHPIALAGHCALCNGTFKWTAENSLGDVLHLNEVTTTTGDRAPILVIRPDVLMAGSEYIFTLNISQSITGLWGSASIALKLNHPPQGGSCTLSPDHSVQLLQDMVSFSCSGWLDEDRECAQMIYSLQELFVGSKENTKMCKDGFFKNIIGKTVVIMSVILLCRNLEVVLPVNDQGTTEWLKKKSQSELLALLQQGNPQDVIQYSTALISQLNQVGGLDFEDRMQMRGNVTQALASLSVSSLQDATQISSALAHSTCYVDDGFSVSQYVLFPLGNSMTAAQKSKDILGANYHYKHQDTSETAVSALQQVGELMRSLMWSRMPGEEALSLKAPQISAVGIRGDPASNLLCTEESSHCQFYIPPALSSKLSKERKEVLQVLLVMKTERHPFVAAADPPISTTLAAMEFGTPQGQPITIANLTSDSAIHVTLHKQDMDMSPRVNVTLPSEGSVNFIVKAVEMYPNAGLFITFNFSLFQAQPQSLLYVTLNSSLSGAEVCVSVCVFSSLCQFFNVEQRRWSTDGLNVLSSSSPNTADCVTQHLTLFGASLFIHPDAIVLLPPVNVVVGIVCGVLLLIHLLVGLIAHKLDHLENLRLSCIPLCGQAGRYHYRVLIKTGWQRGAGTSAHVGIRLYGLNKSGSRHLQKEGAFQRNGLDDFQVETDANLGEIWKICIWHDNTGLDPSWYLQHVVVWDMQMDNMFFFVVDDWLSVENERNSGMVQKVVLATCPQELQQFGRMLRAQLLFGLREHHLWLSLWERPAHSSFSRAQRVTSCALLLHLYLAAGAVWYGAVSTKSGSFVVITPGVARSEPGMMLPSWVLAVVYLLVAVLLGTCLSIVGLYGSKFSSSVVLMWLTSVLSAFFTSALLLEPLKICVRALYLAAVVKPVDPEVEDRLAQETAVRRMVEKQGTKVRPPCGYGLLQAKEEARKVRMVQALMRVSKPHNKDSLSAEFLYSSVEGFRTIWQTNQSLLGLLRGRRVLHELCERHPVLGPLYCLSTFGVGFWLLGRLCGAVLVHTYRTFQAEMYRPSMEPQDYEMVEFLIKRLKLWMGLSKAKEFRHRVKFEGMESPPSRSSRCSQFSSLSVANSPISPSIPSSPAGLRLISSASSLASECSTLSDSPDLQQYVDRLLPSVDSLLAGFDRVNQLTDDVLNIELQLQKASCRIAEKRKKHKDPQPQPQNLKSEVSQLDESPQHMELKPPLSSQQGIHRIIPRRRGTHSESSIMGPSTHVNAHPGNQSWQQGQTVAELDIRNLPRLRAWNSGSCHSADVIQRSPKTQNPAAILVRPRSEEGDRSERSKGVPIKRRAWHPEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DRF4</accession>
    <name>A0A3B4DRF4_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_017551099.1">
        <property type="nucleotide sequence ID" value="XM_017695610.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DRF4"/>
    <dbReference type="Ensembl" id="ENSPNAT00000007368.2">
        <property type="protein sequence ID" value="ENSPNAP00000025544.1"/>
        <property type="gene ID" value="ENSPNAG00000010661.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108426257"/>
    <dbReference type="GeneTree" id="ENSGT00800000125320"/>
    <dbReference type="OMA" id="RTYYIHA"/>
    <dbReference type="OrthoDB" id="2157866at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF27">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="16"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000025544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="122" mass="13289" checksum="637A4262544E9FB1" modified="2018-12-05" version="1">MAVVSATSNGAIEDAEGSKAGWLSKKTQFTHRWRPAWFQIQQSKLLYGANEKTAAKTISLIGARVEAQEADGCFGWTITPKNSKRTFFLRASSAEEQQAWMVAVCAAQIKSGEHAPNACVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DTL0</accession>
    <name>A0A3B4DTL0_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Asteroid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FAM120B</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the constitutive coactivator of PPAR-gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017546176.1">
        <property type="nucleotide sequence ID" value="XM_017690687.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017546177.1">
        <property type="nucleotide sequence ID" value="XM_017690688.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_037394654.1">
        <property type="nucleotide sequence ID" value="XM_037538757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DTL0"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000026803"/>
    <dbReference type="Ensembl" id="ENSPNAT00000004208.2">
        <property type="protein sequence ID" value="ENSPNAP00000026803.1"/>
        <property type="gene ID" value="ENSPNAG00000012134.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108423323"/>
    <dbReference type="CTD" id="84498"/>
    <dbReference type="GeneTree" id="ENSGT00530000063168"/>
    <dbReference type="OMA" id="MPWEVFD"/>
    <dbReference type="OrthoDB" id="25987at2759"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18672">
        <property type="entry name" value="PIN_FAM120B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026784">
        <property type="entry name" value="Coact_PPARg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15976">
        <property type="entry name" value="CONSTITUTIVE COACTIVATOR OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15976:SF17">
        <property type="entry name" value="CONSTITUTIVE COACTIVATOR OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="608"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="633"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000026803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="687" mass="78362" checksum="6B99A0F67C2BE2FC" modified="2018-12-05" version="1">MGVKGLQYFMEACCPETCLPVDLRQMAAAHAKAHPDSTATVVVDAMACLRYWYQCHAWVHGGQWKEYMHLLEEFVGAFTAAGLRLVFFFDGTIEEQKRAEWVKRRLRVNQDIARVFQYVKNHHQQPDREMFCLPSGLATFSRFALKSLGQETWCSVREVDYEIAQYALSNNCMCILGQDTDFVIYDTVSYLSIAKLRLDNMTTVQFCREKLCHILKLQKSDLPLLACILGNDVVPEQQLQYVRKNALTSYGKKHQGDKVHAVADFISRLHLNVDGTLGFSLVHLSKADKEVTEKGMQSYLLPGQTSPWLDCSLPPPEPVCVMQKYMNADILQAAKEKHVQAECFMIYNVLHDGVVDCSNTLEDEEEVELPPQAILYQPVREHIYSILLPALPDSYDHTFTVKEWFVFPGNPLKEPIKVGPRLLNLPERAPDLATLWFGNDSEVKGVRLSTFLAVFDLQDFTEDLNHLDSPLVAVICFVTYVALQAKHLSLEDIDAYLSQAVCIRYKSYSELQQTRVPEVDPRAVQLGSLLLRGLTYIIAANSACGFPFPMSELMPWKTFDGLLFHSKYLQAHSGCSKEELLEGNPAWTTLFLSLRELVLGACRRRGSTIPSQPRRVQHGRPMDGKPHRASGPQPFSTSDQYYRQQTRGPPPCMQGYVHRPRSRHPNRRRYHLAPRWPQYQNQRPGFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4DZM6</accession>
    <name>A0A3B4DZM6_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytokine-inducible SH2-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CISH</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017568130.1">
        <property type="nucleotide sequence ID" value="XM_017712641.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B4DZM6"/>
    <dbReference type="STRING" id="42514.ENSPNAP00000029862"/>
    <dbReference type="Ensembl" id="ENSPNAT00000017362.2">
        <property type="protein sequence ID" value="ENSPNAP00000029862.1"/>
        <property type="gene ID" value="ENSPNAG00000016230.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108436260"/>
    <dbReference type="CTD" id="767678"/>
    <dbReference type="GeneTree" id="ENSGT00940000157392"/>
    <dbReference type="OMA" id="QYSCAQF"/>
    <dbReference type="OrthoDB" id="10063348at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP001501920">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045647">
        <property type="term" value="P:negative regulation of erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045620">
        <property type="term" value="P:negative regulation of lymphocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046426">
        <property type="term" value="P:negative regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd10718">
        <property type="entry name" value="SH2_CIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000042">
        <property type="entry name" value="Cytokine-inducible SH2-containing protein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000002">
        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035887">
        <property type="entry name" value="CIS_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF9">
        <property type="entry name" value="CYTOKINE-INDUCIBLE SH2-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="63"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="175"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSPNAP00000029862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001501920"/>
        </source>
    </evidence>
    <sequence length="224" mass="24942" checksum="C867C6848ED5DBFC" modified="2018-12-05" version="1">MILCVQGPRALLPDSQTQVVPLGVSVISSSSPRCLHNATKIWDPSKDLRAIASTFCYLDASGWYWGAITAGEAHSVLQGALEGTFLVRDSSHPLYMLTLSVKTGRGPTNVRIEYSQGCFRLDSSSPARTRLLSFPDVPSLVQHYVGSRRGKEVSEEDEKDSKVTPVPKECAVLLKLKRPLHRPQAFPSLQHLTRLAINRYTTCPENLPLPRPILQYLQDYPFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B4E004</accession>
    <name>A0A3B4E004_PYGNA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 15">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">HACD3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Pygocentrus nattereri</name>
        <name type="common">Red-bellied piranha</name>
        <dbReference type="NCBI Taxonomy" id="42514"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Characiformes</taxon>
            <taxon>Characoidei</taxon>
            <taxon>Pygocentrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2020-10" db="EMBL/GenBank/DDBJ databases">
            <title>Pygocentrus nattereri (red-bellied piranha) genome, fPygNat1, primary haplotype.</title>
            <authorList>
                <person name="Myers G."/>
                <person name="Meyer A."/>
                <person name="Karagic N."/>
                <person name="Pippel M."/>
                <person name="Winkler S."/>
                <person name="Tracey A."/>
                <person name="Wood J."/>
                <person name="Formenti G."/>
                <person name="Howe K."/>
                <person name="Fedrigo O."/>
                <person name="Jarvis E.D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [L
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